LOCUS       DBNGSG010000001         5057 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3905552, whole genome shotgun sequence.
ACCESSION   DBNGSG010000001 DBNGSG010000000
VERSION     DBNGSG010000001.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5057)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5057)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5057
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3905552"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="AC158Q_00005"
     CDS             complement(<1..918)
                     /locus_tag="AC158Q_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3338179.1"
                     /translation="MQQGFNFELGETNDILRETVSAFAQREIAPRAAGIDRANEFPRD
                     LWPRLGELGLLGITVEEEFGGAGMGYLAHIVAMEEVSRGSAAVGLSYGAHSNLCVNQI
                     RRNGNAPQKQRYLPKLISGEHVGALAMSEAGAGSDVVGMRLRAEKRGGRYVLNGTKMW
                     ITNGPDADVLVVYAKTEPDAGARGITAFLVEKGMKGFSTAQKLDKLGMRGSDTAELVF
                     QDCEVPEENVLGRPNEGVKVLMSGLDYERAVLAGGPLGLMQACMDVVLPYVHERKQFG
                     EPIGSFQLVQGKIADMYTTMNASRAYVYAV"
     gene            complement(1080..1484)
                     /locus_tag="AC158Q_00010"
     CDS             complement(1080..1484)
                     /locus_tag="AC158Q_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR3338180.1"
                     /translation="MITTYTISELAREFGVTTRTIRFYEDQGLISPKREGTARVFSSR
                     DRVRLKLALRGKRLGFSIAEIRELFELYDVSRDEKKQLEEFLSRLDRRRAHLEQQRED
                     IEVMLNEINFFANQCRRLLKVKADDGSTKEAA"
     gene            1573..3378
                     /locus_tag="AC158Q_00015"
     CDS             1573..3378
                     /locus_tag="AC158Q_00015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHA/PHB synthase family protein"
                     /protein_id="HIR3338181.1"
                     /translation="MTEKQQPEQRKFDPAQQAKLYADIAEKSGRLVTQFLERSRNGGV
                     PGLNDELGLAQAFFQAWSKVLSDPFKLAEAQLRFFQDTAALWQSSLMKLMGQDTKAVA
                     EPHQGDRRFKHDDWRENFLYDYIKQSYLIAARHLHQTVGKVEGLDEQTAKKVDFYTRQ
                     YIDALSPSNFLLTNPEVLRETVASGGQNLVKGLNNLLEDVARGGGDQVRTRMTDESAF
                     KLGVNIANTPGKVVFQNELIQLIQYYPANGPVYRRPLLIIPPWINKYYILDLREKNSY
                     IKWAVEQGHTVFVISWVNPDEKLAHKSFEDYLTEGMLAALGAIEKATGEREVNAVGYC
                     LGGTLLACTLGYLAGKKDGRIVSATFLASLIDFEEGGELEVFIDEQQVAALEKRMKKR
                     GYLEGSEMAHTFNMLRANDLIWSFVINNYLLGRDPFPFDLLYWNSDSTRMPATMHSYY
                     LRNMYLKNLLAKPGGIALGGVPIDLGRVETPLYFASTQEDHIAPWRSTYAGAKLFPGA
                     VRFVLGGSGHIAGIVNPPTANKYSYWTNPRFADNPEQWLKGASQHPGSWWTDWAGCVA
                     QHGGDKVPQRVPGKGRLKAIEDAPGSYVRMRVDKG"
     gene            3419..4387
                     /locus_tag="AC158Q_00020"
     CDS             3419..4387
                     /locus_tag="AC158Q_00020"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3338182.1"
                     /translation="MKSKCLLTTAALMAALAGNLMAQTFPVKPVRIIVPFAPGGNVDL
                     VARGVGQRLAEVIGQQVIVDNRPGASALLGTHLVAKSAPDGYTLLAMANTFAVVPSIM
                     SNPGYDPMKDFAGVSQTCLVPQVLVVNTALPVRSLKQLVALAKARPGEMSYASAGVGG
                     TGHMAAALFGSHAGLKMLHVPYKGNAQAIIDVISGQVTMMFDQVSTSTPHIRAGKLRG
                     LAVTSLKRSPLLPDLPTIDESGVRGFEDITFNGLLAPAGTPREVLVRLNAAVAKAVAV
                     PALRQRFIEHGVELTASSSPEAFMDYIRAEFVKKAKLAREVGIRIE"
     gene            4471..>5057
                     /locus_tag="AC158Q_00025"
     CDS             4471..>5057
                     /locus_tag="AC158Q_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017335568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3338183.1"
                     /translation="MQVAGRTFLVTGGASGLGAATARRLAAAGGNTVIADTNAQQGDK
                     LALELGARASFSQADVTDEAQVTKAVAAARKQFGALHGAVNCAGIAPGERVLGKSGPH
                     RLDSFRRAIEVNLVGTFNVLRLAAQAMAENQPDAEGERGIIINTTSIAAYDGQIGQAA
                     YSASKAGVAGLTLPAARELARAGIRVMAIAPGIFET"
BASE COUNT          948 a         1616 c         1622 g          871 t
ORIGIN      
        1 caccgcgtac acgtaggcgc gggaggcgtt catggtggtg tacatgtcgg ctatcttgcc
       61 ctgcaccaat tgaaagctgc cgatcggctc gccgaactgc ttgcgctcgt gcacgtaggg
      121 cagcaccacg tccatgcacg cctgcatcag cccgagcggg ccgccggcca gcaccgcccg
      181 ctcatagtcg aggccgctca tcagcacctt cacgccctcg ttgggccggc ccagcacgtt
      241 ctcctccggc acctcgcagt cctgaaacac cagctcggcg gtgtcggagc cgcgcatgcc
      301 gagcttgtcg agcttctgcg cggtggagaa acccttcatc cccttctcga ccaggaacgc
      361 ggtgatgccg cgcgcgcccg cgtccggctc ggttttggcg tagacgacga gcacgtcggc
      421 atcgggcccg ttggtgatcc acatcttggt gccgttcagc acgtagcggc cgccgcgctt
      481 ttcggcgcgc agccgcatgc cgacgacgtc ggaaccggcg ccggcttcgc tcatcgccag
      541 cgcgccgacg tgctcgcccg aaatgagctt cggcaggtag cgctgcttct gcggggcgtt
      601 gccgttgcgg cggatctggt tcacgcacag attggaatgc gcgccgtagg aaagcccgac
      661 tgcagcggag ccgcggctca cttcctccat cgcgacgata tgggcgaggt agcccatgcc
      721 tgcgccgccg aattcttcct cgacggtgat gcccagcagg ccgagttcgc ccagccgggg
      781 ccacaagtcg cgcgggaatt cattggcgcg gtcgatcccg gccgcgcgcg gcgcgatctc
      841 gcgctgggcg aaagcggaaa cggtttcgcg cagaatgtcg ttggtttcgc ccagctcgaa
      901 attgaaaccc tgctgcatgg gactattcct tggcctaccc gcccattgcg tgcctaaaac
      961 ggcggaaaga ccgcgccatt agggggggcc gcatcggtag tgtaaacgcc agtgttaatg
     1021 gggtttcctg tattaccttt acgttaacgt aaactatgct tgcgctgcga caatcaagcc
     1081 taggcagcct ccttcgtgct gccgtcgtcg gccttgactt tcaacagcct gcggcactga
     1141 ttggcaaaga agttgatctc gttcaacatg acctcgatat cctcgcgttg ttgctcgaga
     1201 tgggcgcgcc gccgatccag cctggagagg aactcctcca gttgcttctt ctcgtcacgg
     1261 gagacgtcgt acagctcgaa cagctcgcgg atctcggcga tggaaaagcc caggcgcttg
     1321 ccgcgcagcg ccagcttcaa tcgcacccga tcgcggctgc tgaagacccg ggcagtgcct
     1381 tcgcgcttgg ggctgatcag gccctggtcc tcgtagaagc ggatggtgcg tgtggtcacg
     1441 ccaaactccc tggcgagttc ggatattgta taagtcgtga tcattgtgct acgcagcaac
     1501 gccatgtaat atagtcgaaa tccagactac acaacgttta cgtaaacgtc aatagcgctt
     1561 gaaagtgtcc caatgaccga aaagcaacag ccagaacaac gcaagttcga ccctgcccag
     1621 caggcgaagc tgtacgccga tatcgccgag aaaagcgggc ggctggtcac gcagttcctg
     1681 gagcgcagcc ggaatggcgg cgtgcccgga ttgaacgatg agcttggact cgcgcaggcg
     1741 tttttccagg cgtggtcgaa agtgctgtcg gacccgttca agctcgccga agcgcaactc
     1801 agatttttcc aggacactgc cgccctgtgg caaagctccc tgatgaagct gatggggcag
     1861 gacacaaaag ccgtcgccga gccgcaccag ggcgatcgcc ggttcaagca cgatgactgg
     1921 cgggagaatt tcctctacga ctacatcaag caatcgtacc tgatcgccgc gcggcatctg
     1981 caccagacgg tgggcaaggt cgaggggctc gacgagcaaa ccgcgaaaaa ggtggatttc
     2041 tacacgcggc agtacatcga cgcgctgtcc cccagcaact ttttgcttac caacccggaa
     2101 gtgctgcgcg agacggtggc gagcggcggc cagaacctgg tgaagggtct gaacaacctg
     2161 ctcgaggacg tggcgcgcgg cggcggcgac caggtgagga cgcgcatgac ggacgagagc
     2221 gctttcaagc tcggcgtcaa catcgccaac acacccggga aagtcgtgtt ccagaacgaa
     2281 ctcatccagc tgatccagta ttacccggca aacggcccgg tttaccggcg gccgctgctg
     2341 atcatcccgc cctggatcaa caagtactac atactcgacc tgcgcgagaa gaactcgtac
     2401 atcaaatggg cggtcgagca ggggcacacg gtattcgtga tctcctgggt caacccggac
     2461 gagaaactcg cccacaagag tttcgaggac tatctgaccg agggcatgct cgcggcgctg
     2521 ggcgcgatcg aaaaagccac cggcgagcgc gaggtgaacg ccgtcggcta ttgcctcggc
     2581 ggcacgctgc tcgcgtgcac gctcggctat ctcgcgggga aaaaagacgg gcgcatcgtg
     2641 tccgcgacct tcctcgcgtc cctgatcgat ttcgaggaag gcggcgagct tgaggtgttc
     2701 atcgacgagc agcaggtggc ggcgctcgaa aaacgcatga agaagcgcgg ctatctcgag
     2761 ggttccgaga tggcgcacac gttcaatatg ctgcgcgcga acgacctcat ctggtcgttc
     2821 gtgatcaaca actacctgct gggccgcgac ccgttcccct tcgacctcct ttactggaac
     2881 tcggattcca cgcgcatgcc ggcgacgatg cacagctact acctgcgcaa catgtacctc
     2941 aagaacctgc tggcgaagcc gggcggcatt gcgctcggcg gcgtgccgat cgacctcggg
     3001 cgggtggaga caccgctcta cttcgcttcc acgcaggagg accacatcgc gccctggcgc
     3061 tcgacctatg ccggcgcgaa gctcttcccc ggagcggtgc gcttcgtgct cggcggctcc
     3121 ggccatatcg ccggcatcgt caacccgccg accgcgaaca agtactccta ctggaccaat
     3181 cccaggttcg ccgacaaccc ggaacagtgg ttgaagggcg catcgcagca cccgggctcg
     3241 tggtggaccg actgggccgg ctgtgtggcg cagcacggcg gcgacaaggt gccgcagcgc
     3301 gtccccggca agggcaggct gaaagccatc gaggacgccc cgggctccta cgttagaatg
     3361 cgcgtggaca aaggctgaac cgtcgcggct ccggtgcgct ttttcatagg gaggggggat
     3421 gaaaagtaag tgcctactaa ccacggccgc actgatggcc gcactggcgg gaaacctgat
     3481 ggcgcagacc ttcccggtca agccggtgcg gatcatcgtg cccttcgcgc cggggggaaa
     3541 cgtggacctg gttgcgcgcg gtgtcggaca gcggcttgcc gaagtcatcg ggcagcaggt
     3601 gatcgtcgac aaccggccgg gcgccagcgc gctgctcggc acccatctcg tcgccaagtc
     3661 ggcgcccgac ggctacacgc tgctcgcgat ggcgaatacg ttcgcggtgg tcccatcgat
     3721 catgtcgaat cccggctacg accccatgaa ggactttgcc ggtgtctcgc agacctgcct
     3781 ggtgccgcag gtgctggtgg tgaacaccgc gcttcccgtg cgttccctga agcagctggt
     3841 cgcgctcgcg aaggcgcggc caggcgagat gtcgtacgcc tcggcgggcg tcggcggcac
     3901 cggccacatg gcggccgcgc tgttcggcag ccacgcggga ttgaaaatgc tgcacgttcc
     3961 ctacaagggc aatgcccagg cgatcatcga cgtcatcagc gggcaggtga cgatgatgtt
     4021 cgaccaggtg agcacgtcga cgccccatat cagggccggg aaactgcggg gactcgcggt
     4081 cacgagcctc aagcgctcgc cgctgctgcc cgacctgccg accatcgacg aatccggcgt
     4141 gcggggcttc gaggacatca cgttcaacgg tctccttgcg cccgccggca cgccccgcga
     4201 ggtcctggtg cgcctcaatg cggccgtcgc gaaagccgtc gccgtcccgg cgctgcgcca
     4261 gcgcttcatc gagcatggcg tcgagctgac cgctagcagc tcgccggaag cattcatgga
     4321 ttacatccgg gccgagttcg tgaagaaggc gaagctcgcg cgcgaagtcg gcatccgcat
     4381 cgagtaacag cgtgacgggt gacgagtgac gagtgaccgg ttttcgattc acgaatcacg
     4441 aatcacgatt cacgccgttg cggccaggcc gtgcaggtag ccggccgcac tttcctcgtc
     4501 accggcggcg cttcgggcct gggcgcggcg acggcgcgca ggctggcggc ggccggcggc
     4561 aacaccgtca ttgccgatac caacgcgcag cagggagaca agctcgcgct cgagctggga
     4621 gctcgcgcga gcttttctca agcggacgtg accgatgaag cccaggttac gaaagccgtt
     4681 gccgcagcgc gaaagcagtt cggcgccctg catggcgcgg tgaattgcgc ggggatcgct
     4741 cccggggagc gggtactggg caagagcggg ccgcatcgcc tcgacagttt tcgccgcgcg
     4801 atcgaggtga acctggtcgg caccttcaac gtgctgcgcc tcgcggcaca ggcgatggcg
     4861 gaaaatcagc ccgatgccga aggcgagcgc ggcatcatca tcaacaccac ctccattgcc
     4921 gcttacgacg ggcagatcgg acaggcggcc tacagcgcgt cgaaggcggg ggtggcggga
     4981 ctgacgctgc cggctgcgcg cgaactcgcg cgcgcgggca ttcgtgtgat ggccatcgcg
     5041 ccgggtatct tcgagac
//
LOCUS       DBNGSG010000002         2768 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3905926, whole genome shotgun sequence.
ACCESSION   DBNGSG010000002 DBNGSG010000000
VERSION     DBNGSG010000002.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3905926"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..950)
                     /locus_tag="AC158Q_00030"
     CDS             complement(93..950)
                     /locus_tag="AC158Q_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3338184.1"
                     /translation="MNSDHILGLGSKGFHRIHYTDWGDPGAGRIVVCAHGLTRTGRDF
                     DDLARALAPDFRVACPDMAGRGRSDWLYAKEDYGYPQYCADMTALIARLTSSGKPAKI
                     YWVGTSMGGIIGMLLASRPNSPIEKLVLNDVGTVIPKAALERIGAYVGQDPRFKTLEE
                     LEAMVRIVCAPFGPLTDAQWRHLTVTGARQHEDGSWGMSYDPGIAAPFRKGPLADVDL
                     WQFWDTITCPTLLLRGAQSDLLLKETAVAMTQRGPRPKLVEFDGIGHAPMLMAGDQIE
                     VVRQFLLGG"
     gene            complement(947..1588)
                     /locus_tag="AC158Q_00035"
     CDS             complement(947..1588)
                     /locus_tag="AC158Q_00035"
                     /inference="COORDINATES: protein motif:HMM:NF046230.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6962 family protein"
                     /protein_id="HIR3338185.1"
                     /translation="MTITEPMTLLTDYVLAGVTGWLGWRLWTTREAFVARRLWSVAFA
                     ALALAALAGGTHHGFTLMLPGTALAALWKMTVLSVGIASFGMLYGSVTATTAGGIRRL
                     LLVLAGAKLALYSLWMLGHDDFIFVIADTGTAMAGIAALHLWSLLRSRDSASRWILGA
                     VGISVLAGAVQAGGYAPHPHFHHNDLYHVIQIVAMILYYKGARLLRDHPATAP"
     gene            complement(1585..2031)
                     /locus_tag="AC158Q_00040"
     CDS             complement(1585..2031)
                     /locus_tag="AC158Q_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338186.1"
                     /translation="MRIGLDRPEHLAPLLWALLALFAARVTGQALVAFLNVRFLPPMQ
                     EWYSGLMPYEYLLPAQIAIIALMTKICLDFTKGRGFFVEPRRFFSVYWLNFGYLYLAA
                     MVLRYPVRMILHPEARWLGGTLPIFFHWVLAVFVIGVGLYHRRHKP"
     gene            complement(2033..>2768)
                     /locus_tag="AC158Q_00045"
     CDS             complement(2033..>2768)
                     /locus_tag="AC158Q_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338187.1"
                     /translation="GDFQYSIDETSDGYLRQGVFSCYRPVADATPMPDVQKELADRDW
                     TDLLYLAHADKAEAFKRYAAYYLSTNGQLYWSDEHQMSVYPDHYHRELDKKLKAKHKA
                     TEAITEIYCERDALERFMADVREDARRNQVNIIYGTIRLIEQDRESFLAWARKPYACV
                     IFNLHVMHTPDGRRHAAGAFRRLIDIGIRHGGSYFPTYHKHATREQVEACYPQFPEFL
                     KLKRKHDPGEVFQSDWYRHYRKMFAA"
BASE COUNT          523 a          848 c          895 g          502 t
ORIGIN      
        1 accgagctct atctcctgat tgccgatgag atcaaggacc ccgccgaacg caagaacttc
       61 acccgcaagg cagtttcgat ctccggcaag cgttagccac cgagcaggaa ttgccggacg
      121 acctcgattt gatcgccggc catcagcatt ggcgcgtgac ctatgccgtc aaattccacc
      181 agcttgggcc gcggcccgcg ctgggtcatg gcgaccgcgg tttccttcaa cagaagatcg
      241 gattgcgcgc cgcgcagcag cagcgtgggg caggtaatgg tgtcccagaa ctgccacagg
      301 tccacgtcgg ccagcgggcc ttttcgaaac ggggcagcga tgcccgggtc gtaactcatg
      361 ccccaggagc cgtcctcgtg ctgccttgcg ccggttacgg tcaaatgccg ccactgggca
      421 tcggtgagcg ggccgaacgg ggcgcaaacg atgcgcacca tggcttccag ctcctcgagc
      481 gtcttgaagc gcgggtcctg accgacgtag gccccgatgc gttccagtgc ggctttgggt
      541 atgaccgtgc caacgtcatt caacaccagc ttctcgatcg gcgagttcgg ccgggaggcg
      601 agcagcatgc ctatgatgcc gcccatggag gtgccgaccc agtagatctt tgcgggcttc
      661 ccgctcgatg tcaggcgcgc aatcagggcc gtcatgtcgg cgcaatactg ggggtagccg
      721 tagtcctcct tggcgtagag ccaatcgctt cggccgcgac cggccatatc ggggcacgcc
      781 acgcggaagt ccggcgcgag cgcccgcgcg aggtcgtcga agtcgcggcc agtgcgggtg
      841 agtccatgcg cgcagacgac gatccgcccg gcgccggggt cgccccagtc cgtgtaatgg
      901 atgcgatgga agcccttgct gccgaggccg aggatgtgat cgctgttcat ggcgccgttg
      961 cggggtgatc gcgtaacagt ctagctcctt tgtagtacag gatcatggca acgatctgga
     1021 tgacgtgata caggtcattg tggtggaagt gcgggtgcgg cgcgtaaccg ccagcttgca
     1081 ccgctcccgc cagcaccgag atgcccacgg cgccgaggat ccaccgcgac gcgctgtcac
     1141 gggagcgcaa cagcgaccag aggtgcaggg cggcaatgcc ggccatcgcc gtcccggtgt
     1201 cggcgatcac gaagatgaag tcatcgtgcc ccagcatcca cagggagtac agcgccagct
     1261 tcgcgcctgc cagcaccagc agcagtctgc gtatgccgcc cgcggtcgtg gcggtgacgg
     1321 atccgtacag catgccgaaa ctcgcgattc ccacgcttag gaccgtcatc ttccacagcg
     1381 cggcgagcgc ggtcccgggc agcatgaggg tgaagccatg gtgggtgccg cctgccagtg
     1441 cagcgagtgc caatgcggcg aacgcgacgg accacagccg ccgggcgaca aacgcttcac
     1501 gcgttgtcca taatcgccag cccagccacc cggttacgcc tgccaggaca taatcggtca
     1561 acagggtcat cggttcggtg atcgtcatgg cttatgtcgc cggtgataga gccccacgcc
     1621 gatgacgaac acggcgagca cccaatggaa gaagatgggc agggtgccgc caagccacct
     1681 tgcctcgggg tgaagaatca tccggaccgg gtagcgcagg accattgccg cgagataaag
     1741 atagccaaag ttgagccagt aaacggaaaa gaagcggcgt ggctcaacga agaagccgcg
     1801 gcccttcgtg aaatccaggc agatcttcgt catcaaggca atgatggcga tctgggctgg
     1861 caggagatac tcatacggca tcagtcccga ataccactcc tgcatcggtg gaaggaaccg
     1921 gacattgaga aatgccacca gcgcctggcc cgttacgcgc gctgcgaaaa gcgccagcag
     1981 tgcccacagc aacggggcga gatgctccgg ccggtcgagc ccgattcgca tctcaggcgg
     2041 cgaacatctt gcggtaatgc cggtaccagt cgctctggaa tacttcgccc gggtcgtgct
     2101 tgcgcttcag cttgaggaac tcggggaact gcgggtagca ggcttcgacc tgttcgcggg
     2161 tcgcatgctt gtggtaagtc ggaaaatagc tgccgccgtg ccgaatgccg atatctatca
     2221 ggcgacggaa tgcgccggcc gcatggcgcc ttccgtccgg cgtgtgcatc acatgcaggt
     2281 tgaagatcac gcaggcatag ggtttcctcg cccacgcaag aaagctttcc ctgtcctgct
     2341 cgatcagccg gatcgtcccg tagatgatgt tgacctggtt gcggcgggcg tcttcccgta
     2401 cgtctgccat gaagcgctcg agcgcatcgc gctcgcaata gatctcggtg atggcctcgg
     2461 tcgccttgtg ttttgccttg agtttcttgt cgagctcgcg gtggtaatgg tcggggtaga
     2521 cgctcatctg gtgctcgtcc gaccagtaga gctggccatt cgtcgagagg tagtaggcgg
     2581 cgtagcgctt gaacgcctcg gccttgtcgg cgtgcgcgag atagagcagg tccgtccagt
     2641 cgcggtcggc cagttctttc tggacgtccg gcatcggtgt cgcgtccgcc accggcctgt
     2701 aacaggaaaa cactccctgc cggaggtagc cgtcggatgt ttcgtcgatg gagtactgga
     2761 agtcgccg
//
LOCUS       DBNGSG010000003         4940 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3226902, whole genome shotgun sequence.
ACCESSION   DBNGSG010000003 DBNGSG010000000
VERSION     DBNGSG010000003.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4940)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4940)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4940
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3226902"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..759
                     /locus_tag="AC158Q_00050"
     CDS             40..759
                     /locus_tag="AC158Q_00050"
                     /inference="COORDINATES: protein motif:HMM:NF012804.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR3338188.1"
                     /translation="MSLIEDLVISYRTLAEEGVIDAYGHVSVRSETNPSRYLMARQLA
                     PELVTEDDILEFDLESNPVDPKGRKFYNERYIHGEIFKARPEVMAVVHNHAPAVVPFS
                     CTSRSTLKPIYHMSAFIGLGVPNWDIRDAQKGSDMLVRTAYLGQSLAKRLGRHPAVLM
                     RGHGSTCVGENLQRAVGRSVYLEQNARMQFQAMMLAGPKGKVVYMDAKEVAANTSWQN
                     YDRSWNLWRTRWLARLKAEKA"
     gene            861..1889
                     /gene="gtdA"
                     /locus_tag="AC158Q_00055"
     CDS             861..1889
                     /gene="gtdA"
                     /locus_tag="AC158Q_00055"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047922 - gentisate 1,2-dioxygenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HIR3338189.1"
                     /translation="MSSAAANPRREQLSGDVARLNMKPLWERVMRLAPGTVAAPVIWR
                     WQQVQPHLMRSAEVITTREAERRVLMLENPALKGSTFATTTLYAGLQVILPGEIARTH
                     RHAPNALRLIVEGTGAYTAVEGERVMMQPGDFVTTPNWAWHDHGNLGSGPVVWLDGLD
                     TAFANLFGAHFREDYPQESQPVSRLEGDSGARFGANLLPVDHRPGGHASPLLTYPYER
                     AREALERLAKGGDPHPSLGHKLRYVNPATGGHVFPTMAVYMQWLPKGFDGRRYRATDG
                     AVFCVVEGGGSVTVGDERWDLAPHDVFAVPSWTHYQFKTDADCVLFSYSDRAAQEALG
                     FWREESQQ"
     gene            2099..2845
                     /locus_tag="AC158Q_00060"
     CDS             2099..2845
                     /locus_tag="AC158Q_00060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - histone deacetylase activity,
                     NAD-dependent [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family NAD-dependent protein deacylase"
                     /protein_id="HIR3338190.1"
                     /translation="MDKLIQFGKLVRSAERGVAFTGAGISTESGIPDFRGPQGVWNTE
                     TPVYYQDFMGDRAARVRAWERGVRMFQRCDSAKPNDGHLVITELQRRGHIASVITQNI
                     DGLHLDAGNENVIELHGTNRFAACQHCWKEWPTSAIVERVEKGDDAPECDACGGPIKA
                     RTISFGQGMPEKEMRLATEATLKADLFVSIGSSLVVEPAASFPRLARQAGARLVIVNA
                     QPTPLDDLADLVIREPIGKTMMALLRQLPG"
     gene            complement(2935..4626)
                     /locus_tag="AC158Q_00065"
     CDS             complement(2935..4626)
                     /locus_tag="AC158Q_00065"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HIR3338191.1"
                     /translation="MAKKRLKATPLPSPVTRHSGKPKRGYSDLHEHLAELKRRGLLLT
                     IDRPIDKDAELHPLVRWQYVGGVEEHERKAFLFTNVVDGLGRKYGFPVVVGAYAGNRE
                     IYCTGMRAKPEEVQPRWDHAIANPLKPRIVTKAPCQDVVIKGAALKGAGRGLDSLPIP
                     VSTPGFDSAPTLTATNVITRDPETGVQNHGTYRAGLKAPDRCVIRMATRVGGAGGYRH
                     YLKHQNRGEKTMPCAIVLGAPPCVAFVAPMKLPLDLDEVGVAGAVAGRPINVVRAKTV
                     DLLVPAEAEIVIEGLIDTEYLEPEGPFGESHGHIAPEEFNMPMRVTAITHRRNAIVAS
                     YLSQVTPSESSAIKRVSYEPMFLTHLRNTLGVRGVKRVTLHERMTALRRIAIVTVEKG
                     TPRTEIWRALTGVSSFKADCGKICIAVNDDIDPDNSDAVFWAIAFRMDPALDLQVLRY
                     RSPGHGPEREHEADREDATLLMDATMKEDLPPLALPKQEYMERARKVWEELGLPKLRP
                     QSPWFGTAAGGADWPQPWDDAAKRGATGRYLENGRISEKQKRKGLKPETRFRPSE"
     gene            complement(4666..>4940)
                     /locus_tag="AC158Q_00070"
     CDS             complement(4666..>4940)
                     /locus_tag="AC158Q_00070"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HIR3338192.1"
                     /translation="GVPTMKETGLPSLEIYTWYGTFAPAGTPAAAVTRINADINALLG
                     QPDIRKLLASQGMVPAGGPPDRLGSLVKSEVPRWKRVVEAAKIKAD"
BASE COUNT          856 a         1626 c         1564 g          894 t
ORIGIN      
        1 cacgaccgga atcaccgctg aatagcgaaa ggcggaaaca tgagcttgat cgaagacctc
       61 gtcatctcct accgaacgct ggcggaggaa ggcgtgatcg acgcctacgg gcacgtcagc
      121 gtgcggtccg agacgaatcc cagccgttac ctgatggcgc ggcagctcgc gcccgagctc
      181 gtgaccgagg acgacatcct cgagttcgac ctcgagagca acccggtcga ccccaagggc
      241 cggaagttct acaacgagcg ctacatccac ggcgagatct tcaaggcgcg gccggaagtc
      301 atggcggtgg tccacaacca cgccccggcg gtggtcccgt tcagctgcac ctcgcgatcc
      361 acgctcaagc cgatttacca catgtcggcc ttcatcggcc tgggcgtgcc aaactgggac
      421 atccgcgacg cccagaaagg cagcgacatg ctggtgcgca cggcgtacct cgggcaatcg
      481 ctcgcgaaaa ggctcggcag gcacccggcc gtgctgatgc gcgggcatgg ctcgacctgc
      541 gtcggcgaaa atctgcagcg cgccgtcggc cgcagcgtct acctcgagca gaacgcccgc
      601 atgcagttcc aggccatgat gctcgccggc ccgaagggca aggtggtgta catggacgcc
      661 aaagaggttg ccgcgaacac gtcgtggcag aactacgacc gctcctggaa cctgtggcgc
      721 acgcggtggc tggcgaggct caaagcagag aaagcgtgac gactagctag cgacgcagtg
      781 acgaacggcc gcttgcctct gatgctatcg gcgttcatcg gcgttcatcg gcggcaaaaa
      841 caggttttca aggaaaatta atgtcatcgg cggcggcgaa cccgaggcgc gagcagctgt
      901 cgggcgacgt ggcgcggctc aatatgaagc cgttatggga gcgcgtcatg cgcctcgcac
      961 ccggcaccgt ggcggcgccg gtaatctggc gctggcagca ggtgcagccc cacctgatgc
     1021 gctctgccga agtgatcacc acccgcgagg ccgagcggcg cgtgctcatg ctcgagaatc
     1081 ccgcgttgaa ggggtccacg ttcgccacca cgacgttgta cgcggggctg caggtcatcc
     1141 tgcccggtga aattgcccgc acgcaccgcc acgcgcccaa tgcgctgcgc ctgatcgtcg
     1201 agggcaccgg tgcctatacc gccgtcgaag gcgagcgggt catgatgcag ccgggcgatt
     1261 tcgtgacgac gcccaactgg gcctggcacg accacggcaa tctcggatcc ggcccggtgg
     1321 tatggctcga cggtctcgat acggcgttcg cgaacctgtt cggcgcgcac tttcgcgagg
     1381 attatccgca ggagagccag ccggtgtcgc ggctggaagg cgattccggc gcgcgattcg
     1441 gcgccaactt gcttcccgtc gatcaccgcc cggggggaca tgcctcgccg ctgctcacct
     1501 acccttacga gcgcgcgcgc gaagccctgg agcggcttgc gaagggcggg gacccccatc
     1561 cttccctcgg ccacaaactg cgctacgtga acccggcgac gggcggccac gtcttcccca
     1621 ccatggcggt atacatgcaa tggctgccga aaggtttcga cgggcgccgc taccgcgcca
     1681 ccgacggcgc cgtgttctgc gtcgtcgagg gaggcgggtc cgtgacggtc ggcgacgagc
     1741 gctgggacct cgcgccgcac gacgtctttg ccgtgccgtc gtggacacat tatcagttca
     1801 aaaccgatgc ggattgcgtg ctgttcagtt attccgatcg cgcggcgcag gaagcgctgg
     1861 gcttctggcg cgaagaatcg cagcaataga caggatttat cagattcaat caaaagcccg
     1921 taaacccgcc aacaatgtca atggacatga ttaacaagat ttacaggatt ggatcaacaa
     1981 aacctaaaca gtttcagagt ctgatgttgt ttgaatcttg ttaatcatgt taatcctgtc
     2041 tattaatggg tttgacgggt tttcctttga ttatcgttaa tcctgtctat tttctgtcgt
     2101 ggataagctg atccaattcg gaaagcttgt ccgctccgcg gagcgtggcg ttgcttttac
     2161 cggcgcgggc atcagcaccg agagcggcat accggacttc cgcgggcccc agggcgtctg
     2221 gaacacggag acgccggttt attaccagga tttcatgggc gaccgcgcgg cgcgcgtgcg
     2281 cgcgtgggag cgcggcgtgc gaatgttcca gcgctgcgac tccgcgaagc ccaacgacgg
     2341 ccacctcgtg atcaccgaac tgcagcggcg cggccatatc gcttcagtca tcacgcagaa
     2401 catcgacggc ctccatctcg acgccggcaa cgaaaacgtg atcgaactgc acggcaccaa
     2461 ccgctttgcc gcatgccagc actgctggaa ggaatggccg acatccgcca tcgtcgagcg
     2521 cgttgagaaa ggcgacgacg cgccggaatg cgacgcctgc ggcggaccga tcaaggcccg
     2581 caccatctcg ttcggccagg gcatgcccga gaaagagatg cggctggcga ccgaggccac
     2641 cctgaaagcc gacctctttg tctcgatcgg ctcgtcgctc gtggtcgaac ccgcggcgtc
     2701 gttcccgcgc ctcgccaggc aggccggggc gcgcctggtg atcgtgaacg cccagccgac
     2761 gcccctcgat gatctcgccg acctcgtgat ccgcgaaccg attggcaaga cgatgatggc
     2821 gctgctgcgg cagttgccgg gctgaacaga ccggacacca gtcacaagtt acaagttaca
     2881 agtttcaaga ttcaagacaa actcctgtaa cttgtacctt gcatcttgta actgttattc
     2941 cgacggcctg aaacgggttt ccggcttgag gcccttgcgc ttctgcttct cgctgatccg
     3001 gccgttctcg aggtagcggc ccgtggcgcc gcgctttgct gcatcatccc agggctgggg
     3061 ccagtccgcg ccgccggcag ccgtgccgaa ccacggcgat tgcgggcgca gcttgggcag
     3121 cccgagctct tcccacacct tgcgcgcccg ttccatgtac tcctgcttgg gcagcgcgag
     3181 cggaggcagg tcttccttca tggtggcgtc catcagcaga gtggcgtcct cgcggtccgc
     3241 ctcgtgctcg cgctcgggcc cgtggcccgg gctgcggtag cgcagcacct gcaggtcgag
     3301 cgccgggtcc atgcggaagg caatcgccca gaacacggcg tcggaattgt ccgggtcgat
     3361 gtcgtcgttc accgcgatgc agatcttgcc gcagtcagcc ttgaagctgg aaacccccgt
     3421 cagcgcccgc cagatctccg tgcgcggcgt gcccttctcc accgtgacga tggcgatgcg
     3481 gcgcagggcc gtcatgcgct cgtgcagcgt cacgcgcttc accccgcgca cgccgagcgt
     3541 attgcgcagg tgcgtgagaa acatgggctc gtacgagacg cgcttgatcg cgctcgactc
     3601 gctcggcgtc acctgggaga ggtaggaggc gacgatggca ttcctgcggt gcgtgatcgc
     3661 cgtgacccgc atcggcatgt tgaattcctc cggcgcgata tggccgtggg actcgccgaa
     3721 ggggccttcc ggctccaggt actcggtgtc gatcagcccc tcgatcacga tctcggcctc
     3781 cgccggaacc agcaggtcca ccgtcttcgc ccgcaccacg ttgatcggcc ggcccgcaac
     3841 cgcgcccgcg acgcccacct cgtccaggtc gagcggcagc ttcatcggcg cgacgaaagc
     3901 gacgcagggc ggcgcgccca gaacgatcgc gcacggcatg gtcttctcgc cgcggttctg
     3961 gtgcttgagg tagtggcggt agccgcccgc cccgcccacg cgcgtcgcca tgcggatcac
     4021 gcagcggtcc ggtgccttga gtccggcacg ataggtgccg tggttctgga cgccggtctc
     4081 cgggtctctg gtgatgacgt tggtcgcggt cagcgtcggc gcgctgtcga atcccggtgt
     4141 cgagaccggg atcggcaggc tgtccagccc cctgcccgcg cctttcaggg ccgcgccctt
     4201 gatcacaacg tcctggcacg gcgctttcgt cacgatgcgc ggcttgagcg ggttggcgat
     4261 ggcgtggtcc cacctgggct gcacttcctc gggcttcgcg cgcatgcccg tgcagtaaat
     4321 ctcgcggttg ccggcataag cgcccaccac cacgggaaac ccgtatttcc gtcccagccc
     4381 gtccacgacg ttcgtaaaca ggaacgcctt gcgctcgtgc tcctcgacgc cgccgacgta
     4441 ctgccagcgc accaacggat gcagctccgc gtccttgtcg atcggccggt cgatcgtgag
     4501 cagcaagccc cgtcgcttca gttccgcgag gtgttcgtga agatccgagt agcctctctt
     4561 cggcttgccg gagtgacggg tgacgggtga cgggagagga gtcgctttaa gtcgtttctt
     4621 ggccatcgaa attcccgtta ttgactctcg tcactcgtca ctgattcaat cagctttgat
     4681 cttcgctgct tccaccacgc gcttccagcg cggcacctcg ctctttacca ggctgccgag
     4741 gcggtcgggc gggccgccgg ccgggaccat cccctggctc gcgagcagct tgcggatgtc
     4801 cggctgcccg agcaacgcat tgatatcggc gttgatgcgg gtcaccgccg ccgcgggcgt
     4861 gccggccggc gcgaacgtgc cgtaccacgt gtatatctcc aggctgggaa ggcccgtctc
     4921 cttcatcgtc ggcacgccgg
//
LOCUS       DBNGSG010000004         2477 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3566512, whole genome shotgun sequence.
ACCESSION   DBNGSG010000004 DBNGSG010000000
VERSION     DBNGSG010000004.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3566512"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="AC158Q_00075"
     CDS             complement(<1..853)
                     /locus_tag="AC158Q_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR3338193.1"
                     /translation="MKPSGSFPLQGVKVIELGTLIAGPFCTRLLAEFGAEVIKVESPG
                     GGDPLRKWRKLHEGTSLWWYAQARNKKSVTLDLKHPEAQDIVRALVKEADIVVENFRP
                     GAMEKWGLGWEVLSKINPRLIMVRLSGFGQTGPYRDRPGFGAIAESMGGLRYVTGYPD
                     RPPVRVGVSIGDSIAALHGVIGALMALHHRNMNGGRGQVVDVALYEAVFNMMESVLPE
                     YGLFGFVRERSGASLPGIVPSNTYVTRDDKYVVIGANADSIFKRMMNAIGRPDLANDP
                     SLAHNDGR"
     gene            1174..1317
                     /locus_tag="AC158Q_00080"
     CDS             1174..1317
                     /locus_tag="AC158Q_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003261220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HIR3338194.1"
                     /translation="MKKFVALLALLMFGALSGCNTMQGLGKDIERGGEKLQNSAKDVQ
                     DGK"
     gene            1343..1597
                     /locus_tag="AC158Q_00085"
     CDS             1343..1597
                     /locus_tag="AC158Q_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HIR3338195.1"
                     /translation="MNWDRVEGNWRQLKGLFKEHWGKITDDQLDIIAGKRERLIGVLQ
                     ESYGLAKDEAERRVKEWEKSEQRAAAFEQALRKNGVSELA"
     gene            complement(2064..>2477)
                     /locus_tag="AC158Q_00090"
     CDS             complement(2064..>2477)
                     /locus_tag="AC158Q_00090"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HIR3338196.1"
                     /translation="EIIDNLEFGLRLTPTEIVAAEVAQGELIRRTARWFEHYDILICP
                     AVSCPPFDVKMRYPETVDGVKLEGYMGWLVLTCAISATSCPVVSLPGGFTTSGLPIGL
                     QLVGPPRSEARLLSMASYVESLLDLQPRTPIDPRP"
BASE COUNT          465 a          754 c          815 g          443 t
ORIGIN      
        1 cccgtccgtc gttgtgggca agcgaggggt cgttcgccag gtcgggcctg ccgatcgcgt
       61 tcatcatgcg cttgaagatg gaatcggcat tggcgccgat gaccacgtac ttgtcgtcgc
      121 gcgtgacgta ggtgttggac ggcacgatgc ccggcagcga cgcgccgctc ctctcgcgca
      181 cgaaaccgaa caggccgtac tccggcagca cgctttccat catgttgaac accgcctcgt
      241 agagtgctac gtcgacgacc tgtccgcgcc cgccgttcat gttgcggtgg tgcagcgcca
      301 tcagcgcgcc gatcaccccg tgcagcgcgg cgatggagtc gccgatcgag acgccgacgc
      361 gcaccggcgg ccggtcgggg tagcccgtga cgtagcgcag cccgcccatg gattcagcga
      421 tggcgccgaa acccggccgg tcgcggtacg gcccggtctg gccgaagccg gagagccgca
      481 ccatgatgag ccgcggattg atcttcgaca ggacttccca gccgagcccc cacttctcca
      541 tcgcgccggg acggaagttc tcgaccacga tgtcggcttc cttcacgagg gcgcgcacga
      601 tgtcctgcgc ttcgggatgc ttcaggtcga gcgtcaccga cttcttgttg cgcgcctgag
      661 cgtaccacca cagcgaagtg ccctcgtgga gcttgcgcca ctttctcagg ggatcgccgc
      721 cgccggggga ctcgaccttg atcacttccg ctccgaactc ggcgagcagg cgcgtgcaga
      781 acggcccggc gatcagcgtg ccgagttcga tcaccttgac accctggagc gggaaggagc
      841 cggatggttt catggcggaa agggattgta gcgattcgcc gcggacctgt tcgctctcgc
      901 gccgggaaaa atcgcgtatt ggtctaccac gcgcgacagg actacgcgcg ccggttcatc
      961 gaaatgtccg ggggatcgcg gcggctgtcg cccgggcacg acgttagacc ccgggcatca
     1021 gtcacggcgg tactgtaacc aattgttttc atggaaatag ccggcctggc acgacctgtg
     1081 cgagtgactg tggcagggtc gtaatcgact ccgcgcggcg tcgtatgtgc cggcgcgcgt
     1141 gacgtacaac acgcttcaag gaaggagctg ccaatgaaaa agttcgtagc gttgcttgcg
     1201 ttgctgatgt tcggcgcgct gtccggctgc aacaccatgc agggtctcgg caaggacatc
     1261 gagcgcggcg gtgagaagct gcagaacagc gcgaaagacg tccaggacgg aaagtaacga
     1321 acattcatac gaggagcacg ggatgaactg ggatagggtc gagggcaact ggcggcaatt
     1381 gaaggggctg ttcaaggagc actggggcaa gattaccgac gaccagctcg acatcattgc
     1441 gggcaagcgc gagcggctga tcggcgtgct gcaggagagt tacggtctcg cgaaagacga
     1501 ggccgaacgc cgggtgaagg aatgggaaaa gagcgagcaa cgcgccgccg ccttcgagca
     1561 ggcgctccgc aagaacggcg tcagtgaact ggcataggaa agccgccagt taccgcagcc
     1621 tggacctgca gccatgccga caccgtgggt gtcgggaaac ggcgacaggg aaagctgccg
     1681 tcgctcaacc agatgccgga ttgcggaatt cagtccgtct ccagctggcg acacttgggc
     1741 ctgggctgcc gcacgcccgg ttttgctcaa catcatgatt gatatcggaa acagcctgct
     1801 ggcacgcccc gtgcatggat ctccggcagg gccctttcgg taacgaacgg acacccaact
     1861 gtgtagttgc agtagttgca tccctcccct gtttaccccg gcatatgccg gggttttttt
     1921 gtactgcagg gatgagggcg gagggatgag ggatgaggga tgaggagtca agcacgagtt
     1981 actttgcctg cccacctccc tgagagtttt ccttcatatt attttggttt cttggcggcc
     2041 ttggcgactt ggcggttcat cttctacggt cgcgggtcga tcggcgtcct gggctgaagg
     2101 tctagcagcg actcgacata tgacgccatc gagagcagcc gcgcctcgct gcgcggcggg
     2161 ccgaccagct gcaggccgat cggcaggccg gacgtggtga accctcccgg cagcgagacc
     2221 accgggcagg aggtggcgct gatggcgcag gtcaggacaa gccagcccat gtagccctcg
     2281 agcttcacgc cgtccaccgt ctccgggtag cgcatcttca cgtcgaacgg cgggcacgag
     2341 accgcagggc aaatcagtat gtcgtagtgc tcgaaccacc gcgccgtgcg ccggatcagc
     2401 tcgccctgcg ccacttcggc agcgacgatc tccgtgggcg tcaacctgag cccgaattcc
     2461 aggttgtcga tgatctc
//
LOCUS       DBNGSG010000005         2536 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2887426, whole genome shotgun sequence.
ACCESSION   DBNGSG010000005 DBNGSG010000000
VERSION     DBNGSG010000005.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2887426"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..1539)
                     /locus_tag="AC158Q_00095"
     CDS             complement(97..1539)
                     /locus_tag="AC158Q_00095"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HIR3338197.1"
                     /translation="MSVFYPRSFPRLLAVGFTLIALPLVFALVNNAISVDRLANRSQN
                     AVYQAAQATQASRRLAQLLGALERTARQILILDDRTLLDDYAANRQRFEQTVAEFARL
                     PLDVEPRIALDEIVSAEAEIFATLSRADVKGAQLKETIERFAGMAERAQSIVASSNVL
                     IDREVTAMRATADQAQRITFWQLLALVPVVTFLVVGFSILITRPFRQVDAAIRRLGAG
                     QLRVPVVVTGPQDLEQLGERLEWMRRELLDLEEQKNRFLRQVSHELKTPLTALREGAE
                     LLSEEVVGKLTAEQREIAEILRHNSVELQKLIEDLLSFGASQFRKVTVDLEPVEIRLI
                     VERVASDQRLATKARGLTLDVAADDIMLSADPEKLRVALDNLISNAIKFSPEGGRIKV
                     AARLVGGALQLDVIDQGPGIAPAERSRIFEPFYQGGHAGAGPVRGTGIGLSVVKEYVF
                     AHGGSVEVVDSQEGAHMRIRVPMTRTEAAA"
     gene            complement(1687..2280)
                     /locus_tag="AC158Q_00100"
     CDS             complement(1687..2280)
                     /locus_tag="AC158Q_00100"
                     /inference="COORDINATES: protein motif:HMM:NF017319.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaC/ttg2D family ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR3338198.1"
                     /translation="MNTIMRVASGLFLAMAVSAQAADTAPDALVKSTVDEVLSVIKQT
                     KDQRALVELAEKKVITHFDFKQMTQLAVGRSWSKANAAQQEALERAFRTLLVRTYTTA
                     LSQSSGQTKVDIKPSPPKQGDAETVVRTLVNEPGRKPFNIDYRMKNGPGGWKVYDVVV
                     ENLSLVTNYRNSFQSEIDRSGIDGLIKAIESKNQKQG"
     gene            complement(2303..>2536)
                     /locus_tag="AC158Q_00105"
     CDS             complement(2303..>2536)
                     /locus_tag="AC158Q_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HIR3338199.1"
                     /translation="TIFRAWAIAGTVILLAGCAGGPQERHGCAQPNYGGAVIGGLAGG
                     LIGAQVGSGSGRTAATAVGAGTGAVIGSQVGCE"
BASE COUNT          440 a          890 c          768 g          438 t
ORIGIN      
        1 cccagggcac gggttcctgg ccatcgagcc cgcgcagggc gccgcacccg gccagcacgc
       61 cgcagccgat gaatacgagc gcggccggcc gcagcatcac gccgccgcct ccgtgcgggt
      121 catgggcacc ctgatccgca tgtgcgcgcc ttcctggctg tccacgacct ccacgctgcc
      181 gccatgggca aacacgtatt ccttcaccac ggagagcccg atgcccgtcc cgcgcaccgg
      241 ccccgcgccg gcatgcccac cctggtaaaa aggctcgaag atgcgcgagc gttccgcggg
      301 cgcaataccc gggccctggt caatcacgtc cagctgaaga gcgcctccga cgaggcgcgc
      361 ggcgaccttg atgcgtccgc cttcggggga gaacttgatg gcattggaaa tcagattgtc
      421 gagcgccacg cgcagtttct ccgggtccgc ggacagcatg atgtcatcgg ccgcgacatc
      481 gagcgtcagg ccgcgagcct tggtggccag tcgctggtcg gaggccacgc gttcgacgat
      541 gagccggatt tccacgggct ccaggtccac ggtcaccttg cgaaactggc tcgcaccgaa
      601 tgacagcaaa tcctcgatca acttctgcaa ttctacactg ttgtggcgca ggatctccgc
      661 aatctcgcgc tgctccgccg tcagctttcc gaccacttcc tccgacagca gttccgcgcc
      721 ttcgcgcagc gcggtgagcg gcgtcttcag ctcgtgcgaa acctgccgca ggaaacggtt
      781 cttctgctcc tccaggtcga gcagctcgcg cctcatccac tccaggcgct cgccgagctg
      841 ctcgaggtcc tgcggcccgg tgaccaccac cgggacccgc agctgcccgg cgccgagccg
      901 gcggatcgcg gcgtccacct gccggaaggg acgcgtgatg agaatggaaa agccgaccac
      961 caggaacgtc accaccggca ccagcgccag cagctgccag aacgtgatgc gctgcgcctg
     1021 gtcggcggtg gcgcgcatcg ccgtgacctc gcggtcgatc aggacgttcg aactcgccac
     1081 gatcgactgg gccctctcgg ccatacccgc gaagcgctcg atcgtctcct tcagctgcgc
     1141 ccccttcaca tcggcccgcg acagtgtcgc gaagatttcg gcttccgcgc tcacgatttc
     1201 gtcgagcgcg attctgggtt ccacatcgag cggcaggcgg gcgaactccg ccaccgtctg
     1261 ctcgaaacgt tgccggttcg ccgcatagtc gtcgagcaga gtgcggtcat cgagtatcag
     1321 gatctggcgg gcggtgcgct cgagcgcgcc gagcagttgc gcaagccgcc ggctcgcctg
     1381 ggtagcctgc gccgcctggt acacggcgtt ctggctgcga ttggcgagcc ggtcgaccga
     1441 gatcgcgttg ttgaccagcg cgaacaccag cggcagcgca atcagcgtga acccgaccgc
     1501 cagcagcctg ggaaacgaac gcggatagaa aacggacatg cagagagatt agcctcccca
     1561 catagaccgg ggcaatgccc aaaagtgccg tgacttagtt cacatggagc ggcaaagact
     1621 tgccaacggc atagtggctc ccacggcgat ccatgacacc ggcccgcgcg cggcgccgcg
     1681 gtcgcgctaa ccctgtttct ggttcttgga ttcgatggcc ttgatcaggc cgtctatgcc
     1741 cgagcggtcg atctcggact ggaacgagtt gcggtaattg gtcacgaggc tgagattctc
     1801 caccaccacg tcatagacct tccagccgcc gggaccattt ttcatgcggt aatcgatatt
     1861 gaagggcttt cggccgggct cgttcaccag cgtccgcacc acggtttccg cgtcgccctg
     1921 cttgggcggc gaaggcttga tatccacctt ggtctggccg ctgctctgag acaacgcagt
     1981 ggtgtaggtg cgcaccagca aggtgcggaa cgcgcgttcg agcgcttcct gctgcgcggc
     2041 gttcgccttc gaccaggagc ggccgaccgc aagctgcgtc atttgcttga aatcgaagtg
     2101 cgtgatgacc ttcttttcgg cgagttccac cagcgcgcgc tggtccttgg tctgcttgat
     2161 gacagagagc acctcatcca ccgtgctctt caccagcgca tcgggcgcgg tgtccgcggc
     2221 ctgtgccgac acggccatcg cgagaaaaag cccgcttgcc acacgcatga tcgtattcat
     2281 gatcgaacct cctgcgataa cttcactcgc agccaacctg gctgccgatc accgcgcccg
     2341 tgcctgcgcc gaccgcagta gccgcggtgc ggcccgaacc gctgccgacc tgcgcaccga
     2401 tcaatccccc agcaaggcct ccaatcaccg cgccgccgta gttcggctgc gcacaaccgt
     2461 gccgctcctg cggcccgccc gcgcaccccg ccagcagaat tacggttccg gcgatggccc
     2521 atgcacggaa tatcgt
//
LOCUS       DBNGSG010000006         1915 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_850305, whole genome shotgun sequence.
ACCESSION   DBNGSG010000006 DBNGSG010000000
VERSION     DBNGSG010000006.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_850305"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="AC158Q_00110"
     CDS             complement(<1..718)
                     /locus_tag="AC158Q_00110"
                     /inference="COORDINATES: protein motif:HMM:NF024138.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR3338200.1"
                     /translation="MSKYAYQAIIPTVRWTAEDNAIDARLLPLLRAVGKTGSLNKAVA
                     ALGFSYRHAWGMVGEMERTLGRSLVVMDRGRGARLSPFAQKLLEADDVATRVIERELA
                     ATVRALGRATPLTLDKPRGMPLVIHASHDYALAELRDRLLARGNPAELHFRGSLECLA
                     SLARRECDVAGFHVPEPSAGNAAFAPYRPLLATRGLRLVRFVNRRQGLIVARGNPKRL
                     LALSDLVAKRVRFVNRQPESG"
     gene            799..1503
                     /locus_tag="AC158Q_00115"
     CDS             799..1503
                     /locus_tag="AC158Q_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3338201.1"
                     /translation="MNEILLAVTEAGRMIARLDSALFEIVLLSLRVSLTAVVLASVIG
                     LPLGAAIAVGRFPGRQALIVMLNALMGLPPVVVGLVVYLMLSRAGPLGEFGILFTPAA
                     MVVAQTILIAPIVAALSRQVIEDAWQEYREQLRSLGAGMTTAALTLLWDTRFSLVTIV
                     LAGFGRAAAEVGAVMIVGGNIDGVTRVMTTAIALETSKGDLPLALGLGMILVTLVLAL
                     NAGAYWIKEAAQRRYG"
     gene            1507..>1915
                     /locus_tag="AC158Q_00120"
     CDS             1507..>1915
                     /locus_tag="AC158Q_00120"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3338202.1"
                     /translation="MLPLALQDVSFAISGKILINRVSMEITAGPRTVILGPNGAGKSV
                     LMRLCHGLFAPTSGSITWRGAANGRRRQAMVFQRPVMLRRSALANVVYALKLAGTSPR
                     ERELRGRDVIDAVGLSGVAERPARVLSGGEQQRL"
BASE COUNT          310 a          610 c          665 g          330 t
ORIGIN      
        1 tccccgattc cggctggcgg ttgacgaaac gaacgcgctt ggcgaccagg tcggagagcg
       61 ccagcagccg cttcgggttg ccgcgggcga cgatcaatcc ctggcgccgg ttgacgaagc
      121 gcaccaggcg caggcctcgc gtggcgagca gcggacggta aggcgcgaac gccgcattgc
      181 ccgctgacgg ctccggcacg tgaaatccgg ccacgtcgca ttcccggcgg gcgaggcttg
      241 cgaggcactc caggctgccg cggaaatgaa gctctgcagg attgccgcgg gccagaagcc
      301 ggtcccgcag ctcggccagc gcgtaatcat ggctcgcgtg gatcaccagc ggcatgccgc
      361 gcggcttatc cagcgtaagc ggggtagcgc gcccgagcgc ccgcacggta gcggccagct
      421 cccgctcgat cacccgggtg gcgacgtcgt cggcttccag cagcttctgc gcgaatgggc
      481 tcaaccgcgc gccgcggccg cggtccatga cgaccagcga gcggcccagc gtccgctcca
      541 tctccccgac catcccccat gcatgacgat aggagaagcc cagcgccgcc acggccttgt
      601 tgagggagcc tgttttaccg accgcgcgca gcagtggcag caggcgcgca tcgatggcgt
      661 tgtcctccgc cgtccagcgg accgttggaa taatcgcttg atatgcatat ttagacatag
      721 gtagaggatc taatgcttat tgaataagct taatttcata agaaaccgat atatgccaag
      781 aaaagcataa cataattcat gaatgagatt ctgctggcgg tcaccgaagc gggccggatg
      841 atcgcgcggc tcgacagcgc gctcttcgaa atcgtcctcc tcagcctgcg ggtgagccta
      901 accgcagtag tgctggccag cgtcattggc ttgcccctcg gggccgcgat agcagtgggg
      961 cgttttccgg gccggcaagc gctgatcgtc atgctgaacg ccctgatggg cctgccgccg
     1021 gtcgtcgtcg gcctggtggt ctacctgatg ctctcgcggg caggaccgct cggcgagttc
     1081 gggatcctgt tcacgcccgc cgcaatggtc gtggcgcaga cgatcctgat cgccccgatc
     1141 gtcgctgcgc tttcgcgcca ggtgatcgag gatgcgtggc aggagtatcg cgagcagttg
     1201 cgctcgctgg gcgcagggat gacgaccgca gccctgacgc tgctctggga tacccggttc
     1261 tcgctggtga cgatcgtgct cgccgggttc gggcgtgccg cggcagaggt gggcgcggtc
     1321 atgatcgtgg gcggcaacat cgacggggtg acgcgcgtga tgaccaccgc catcgcgctc
     1381 gagacgagca aaggcgacct gccgctggcg ctgggtctgg gcatgatcct cgttacgctg
     1441 gtgctcgcgt tgaatgcggg cgcgtactgg atcaaggaag cggcgcaacg ccgctacggg
     1501 taagccgtgc ttccgctcgc cttgcaggac gtgtcattcg cgatctccgg caagatcctc
     1561 atcaaccgcg tttccatgga gatcacggca gggccgcgca ccgtcatcct cgggccgaac
     1621 ggggcgggga agagcgtgct catgcggctg tgccacgggt tgttcgcccc cacgtcgggg
     1681 agtatcacct ggcgcggtgc ggcgaacggg cggcgcaggc aggccatggt attccagcgc
     1741 ccggtgatgc tgcgccgctc ggcgctggcc aacgtggtct atgcgttgaa gctcgcgggc
     1801 acttcgcccc gcgagcgtga gttgcgcgga agggacgtta tcgacgcggt cgggctctcc
     1861 ggcgtcgccg agcggccggc gcgggtgctg tccggcggcg agcagcagcg gctcg
//
LOCUS       DBNGSG010000007         4397 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4416384, whole genome shotgun sequence.
ACCESSION   DBNGSG010000007 DBNGSG010000000
VERSION     DBNGSG010000007.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4397)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4397)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4397
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4416384"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="AC158Q_00125"
     CDS             <1..529
                     /locus_tag="AC158Q_00125"
                     /inference="COORDINATES: protein motif:HMM:NF024860.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HIR3338203.1"
                     /translation="SGRDGAAPREPQSGSCLRLIRDLVPDVKFIHIHRDPVQVAHSLV
                     RKGWIGTMRDGLEIWRQYLAEITGFLAELPDEQQLTVGHAELLTAPDDSALAIAQFLG
                     LAGHEAILGFLDRQRHTPTPFSEPVSDFSRPFGLRFDRAPVSLGPATELAARLGYAET
                     ASGSISRAGPDAPGA"
     gene            complement(715..2451)
                     /locus_tag="AC158Q_00130"
     CDS             complement(715..2451)
                     /locus_tag="AC158Q_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR3338204.1"
                     /translation="MKPSDTTLARTAAASPQASGAPAPRTGGQILIDALKVHGVDIAF
                     GVPGESYLEVLDALHDAREKMQFIICRQEGGAAYMAEAYGKLTGRPGICMVTRGPGAT
                     NAAIGVHVAQQDSTPFILFVGQVGNDFVERDAFQEVDYRRMFGQMTKWVAQIDRADRI
                     PEYVNRAFHTAMSGRPGPVVLALPEDMLTETASVADTAPYKRVAAHPGEGDLRKLREL
                     LAQSKRPFVILGGSGWNAQACEDFRSFAEVNNLPVGTAFRRQDLYDNRLPNFAGDVGI
                     GITPALAAHIKEADLILAVGTRLDEMTTGGYTLIGVPRPKQKFVHVHAGAETLGRVYE
                     SDLPIHSGMPEFAAALRKQQPITSSAWGDWTKAARKDYEAWLEPAPVPGNLDMAKVMV
                     YLRKRLPPETIVANGAGNFAGWVHRYYPYPGFRTEVAPIAGSMGYGAPAGIAAKLVHP
                     ERPVISFCGDGDFLMNGQELATAAQYGVKVVFLVVNNGMYGTIRMHQEREHPGRVSGT
                     DLKNPDFAALARAYGLHGEIVEKTADFEAAFERCWSATTASLIELKIDPEAITTRTTL
                     SAIRANALKNKK"
     gene            2587..3999
                     /locus_tag="AC158Q_00135"
     CDS             2587..3999
                     /locus_tag="AC158Q_00135"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR3338205.1"
                     /translation="MQELWKLSAVEVVGLLRQKKVSPLELIDVAAARIEATNPKINAF
                     VTLCYDRAREHAGRLAKSPPAEVPPHYLYGLPIGVKDGTDVAGVRCTSGSRIYADRIA
                     PASDIVVERLEANGAIVIGKTNLPEFAAGGNTFNDVFGATLNPWDVRTTSSGSSGGSA
                     AALASGQVWFATGGDFGGSIRTPSSYCSTTGLRPSPGMVPRASKQPYNPLSVEGPMAR
                     NVADTALMFDCEAGWHPLDPLSQVGPHPSFSAAAARPKKPLRVAFSTDLGIARAVDRE
                     VRGVCKAAAEKLARDGVAVEEAHPDLADAEKTFLTLRGAVFIGRHAELMKKHRHLYKQ
                     EIIDNLEFGLRLTPTEIVAAEVAQGELIRRTARWFEHYDMLICPAVSCPPFDVKMRYP
                     ETVDGVKLEGYMGWLVLTCAISATSCPVVSLPGGFTASGLPIGLQLVGPPRSEARLLA
                     MASYVESLLDLQPRTPIDPH"
BASE COUNT          741 a         1390 c         1477 g          789 t
ORIGIN      
        1 ctccggccgc gatggcgccg caccgcgcga gccgcaatcg ggctcctgcc tgcgcctcat
       61 ccgcgacctt gtgccggacg tcaaattcat ccatattcac cgggacccgg tccaggtcgc
      121 gcattcgctc gtgcgcaagg gttggatcgg cacgatgcgc gacgggctgg aaatctggcg
      181 ccaatacctt gccgagatca ccggcttcct ggcggaattg ccggacgaac agcaactgac
      241 ggtcgggcat gccgaactgt tgaccgcgcc cgatgactcg gcactggcga tcgcgcaatt
      301 tctcggtctt gccgggcatg aagcgatcct ggggttcctg gacaggcaac ggcacacgcc
      361 gacgcctttc agcgagccgg tgagcgattt ttccagaccg tttggactgc gtttcgatcg
      421 cgcccccgtt tccttgggcc cggcaacgga actggctgcc cggctcggct atgcggaaac
      481 ggcatcaggc tcgatttccc gcgccggccc ggacgcacca ggcgcctaac aacgaccggc
      541 tattttcctg ctgcattctc tgtcacaggc ctgccggcag gtcaggagct tctggatgcc
      601 ggattcggtc aggtatctga tcgtgtcgaa tgagtgactc gtttttcgtt tctcgttgct
      661 cgtggtttga tcctgaaccc tgccgaaaac gagaaacgag gaacgagaaa cgaatcactt
      721 cttgttcttg agggcattcg cccgaatggc cgaaagagtc gtgcgggtgg tgatcgcctc
      781 cgggtcgatc ttcagctcga tgagggaagc ggtcgtcgcg ctccagcagc gctcgaacgc
      841 cgcctcgaag tcggcggtct tctccacgat ctcgccgtgc agcccgtagg cgcgggcaag
      901 cgcggcgaaa tcgggatttt tcagatcggt tccgcttacg cgcccggggt gctcgcgctc
      961 ctggtgcatg cggatggtgc cgtacatgcc gttgttgacg acgaggaaca cgactttcac
     1021 gccgtactgc gcagcagtcg cgagttcctg gccattcatc aggaaatcgc cgtcgccgca
     1081 gaacgagatc accggccgct cgggatgcac cagcttggcg gcgatgccgg ccggtgcccc
     1141 gtagcccatc gagccggcga tcggtgcgac ctcggtgcga aaaccgggat aggggtagta
     1201 gcggtgcacc cagcccgcga agttgccggc accgttggcg acaatggtct cgggcggcag
     1261 ccgcttgcgc aggtagacca tcacctttgc catgtcgaga tttcccggaa ccggggccgg
     1321 ttcgagccac gcctcgtaat ccttgcgcgc ggccttcgtc cagtcacccc atgccgatga
     1381 ggtgatcggc tgctgcttgc gcagggccgc agcgaactcc ggcatgccgg aatggatcgg
     1441 caggtcggac tcgtacaccc gccccagcgt ctcggcgccg gcatgcacgt gcacgaactt
     1501 ctgcttcggg cgcggcacgc cgatcagggt gtagccgccg gtggtcatct cgtccaggcg
     1561 cgtgccgacg gcgagaatga ggtcggcttc cttgatgtgc gcggcgagcg cgggagtgat
     1621 gccgataccg acgtcgccgg cgaagttcgg cagccggttg tcgtagaggt cctggcggcg
     1681 gaaagcggtg ccgaccggca ggttgttcac ttccgcgaaa ctgcgaaaat cctcgcacgc
     1741 ctgggcgttc cagccgctgc cgccgaggat cacgaacggg cgcttcgatt gcgcgagcag
     1801 ttcgcgcagc ttgcgcaggt cgccctcgcc ggggtgcgcg gcaacgcgct tgtagggcgc
     1861 ggtgtcggcg acgctggcgg tctcggtcag catgtcctcc ggcagcgcga gtaccaccgg
     1921 gccgggcctt cccgacatgg cggtgtggaa cgcgcggttc acgtactcgg gaatacggtc
     1981 ggcgcggtcg atttgcgcga cccacttcgt catctggccg aacatgcggc ggtaatcgac
     2041 ttcctggaac gcgtcgcgct cgacgaaatc gttgccgacc tggccgacga agaggatgaa
     2101 cggggtcgag tcctgctgcg ccacgtgcac gccgatcgcg gcattggtcg cgcctgggcc
     2161 gcgggtgacc atgcagatgc cgggccggcc ggtcagcttg ccgtaggcct ccgccatgta
     2221 ggccgcgccg ccttcctggc ggcagatgat gaactgcatc ttctcgcgcg cgtcatgcag
     2281 cgcgtcgagc acctccaggt agctctcgcc gggcacgccg aatgcgatgt ccacgccatg
     2341 gaccttgagc gcatcgatca ggatttgccc accggtgcgc ggagcagggg ccccacttgc
     2401 ttgcggggat gcggccgcgg tgcgggcgag cgtggtatcg gagggtttca tggggaagtc
     2461 ctgtcggaaa cggccgggca aaggcaggcc gcaaatctat cacggatgcg cacgcattat
     2521 ccacgggcgc gtgaatgctt ggcgatacaa tccattttcg gcataccgag aaatccggga
     2581 ggagcgatgc aggaattgtg gaagctgtcg gcggtggagg tcgtggggct gctccggcag
     2641 aagaaggtct cgccgctgga actgatcgac gttgccgcgg cccggatcga ggcgaccaac
     2701 ccgaaaatca acgctttcgt caccttgtgc tacgaccggg cgcgggagca cgccggacgc
     2761 ctggcaaaat cgcctcccgc cgaggtgccg ccgcactact tatacggact gccgatcggg
     2821 gtgaaggacg gcaccgatgt tgcgggcgtg cgctgcacgt ccggttcgcg catctacgcc
     2881 gaccgcatcg cgcccgcgtc ggatatcgtc gtggagcgac tggaggccaa cggcgcaatc
     2941 gtcatcggca agaccaacct gcccgagttc gcggcgggcg gcaacacgtt caacgacgtg
     3001 ttcggcgcga cgctgaaccc gtgggacgtg cgcacgacat cgagcggctc ttccggcggc
     3061 tcggcggcgg cgctggcctc gggacaggtg tggttcgcga ccgggggcga cttcggcggc
     3121 agcatccgca cgccgtcgag ctactgctcg accaccgggc tgcgcccgag cccgggcatg
     3181 gtgccgcgcg cgtcgaagca gccgtacaac ccgctctcgg tcgagggccc gatggcgcgc
     3241 aacgtcgcgg acacggcgct catgttcgat tgcgaagcgg gatggcatcc gctcgatccc
     3301 ctgtcccagg tcggtccgca cccgagcttc tctgcggcgg cggcgcgacc gaagaagccc
     3361 ctgcgcgtcg ccttcagcac cgatctcggc atcgcgcgcg cagtggaccg cgaagtgcgc
     3421 ggcgtatgca aggcggcggc ggagaagctc gcgcgcgacg gcgtggcggt ggaggaggcc
     3481 caccccgatc ttgctgacgc ggagaagacc tttctcacgc tgcgcggcgc ggtgttcatc
     3541 gggcgccacg cggaactgat gaagaagcac cgccacctgt acaagcagga gatcatcgac
     3601 aacctggaat tcgggctcag gttgacgccc acggagatcg tcgctgccga agtggcgcag
     3661 ggcgagctga tccggcgcac ggcacgttgg ttcgagcact acgacatgct gatttgcccc
     3721 gcggtctcgt gcccgccgtt cgacgtgaag atgcgttacc cggagacggt ggacggcgtg
     3781 aagctcgagg gctacatggg ctggcttgtc ctgacctgcg ccatcagcgc cacctcctgc
     3841 ccggtggtct cgctgccggg agggttcacc gcgtccggcc tgccgatcgg cctgcagctg
     3901 gtcggcccgc cgcgcagcga ggcgcggctg ctggccatgg cgtcatatgt cgagtcgctg
     3961 cttgaccttc agcccaggac gccgatcgac ccgcattgat tactgtgttg agtgtttagt
     4021 tttgagtgtt gcgttggaag cgcatttcgc tcatcactta gaactcaaaa ctcaaaactg
     4081 aagtacaaaa aaagaccccg gcatatgccg gggtaaacag gggagggatg caactactgc
     4141 aactacacag ttgggtgtcc gttcgttacc gaaagggccc tgccggagat ccatgcacag
     4201 ggcgtgccag caggctgttt ccgatatcaa tcatgacgtt gagcaaaacc gggcgtgcgg
     4261 cagtccaggt ccaagtgtcg ccagctggag acggactgaa ttccgcaatc cggcatctgg
     4321 ttgagcgatg gcagctttcc ctgtcgccgt ttcccgacac cacggtgtcg gcatggctgc
     4381 aggtccaggc tgcggta
//
LOCUS       DBNGSG010000008         3005 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1020412, whole genome shotgun sequence.
ACCESSION   DBNGSG010000008 DBNGSG010000000
VERSION     DBNGSG010000008.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1020412"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            381..1712
                     /locus_tag="AC158Q_00140"
     CDS             381..1712
                     /locus_tag="AC158Q_00140"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019248572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose dehydrogenase family protein"
                     /protein_id="HIR3338206.1"
                     /translation="MKLSVIGTGYVGLVTGACFAEMGNTVACLDTDSARVEALRAGKV
                     PFYEPGLAELVASNGREGRLRFTSSHAEAIADAQVHFIAVGTPQGENGAADLTHVLEA
                     ARQIGRHALQPCVVVDKSTVPVGTAERVRAVIGEELARRRASVRFEVVSNPEFLKEGA
                     AVADFMRPERVVIGTDSAEAAETLRQLYAPFTRSHERLLVMGVRDAELTKYAANAMLA
                     TRISFMNEIAGLCERLGVDVENVRRGIGSDSRIGHAFIYPGCGYGGSCFPKDMTALIH
                     MAKSSGLAPGLLQAVEARNQSQKQRLYEKIAERFGAGLSGRVIALWGLAFKPGTDDLR
                     EAPALALIERLLEAGAAVHAYDPVAMDNARRSLPRAWQQAGRLEFAATQYDAVAGADA
                     LALVTEWKPFRQPDFDSMKKLMKTPVIFDGRNQYDPGQARAAGFEYFGIGR"
     gene            1768..2493
                     /locus_tag="AC158Q_00145"
     CDS             1768..2493
                     /locus_tag="AC158Q_00145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3338207.1"
                     /translation="MAHPAITLSVVSHGQNALVNQLIGDVARHCAGSVALVLTQNAPD
                     AVPLAAADRLPGLDVVVNERPKGYGANHNAAFARCRTPYFCVANPDIRLPSDPFPPLL
                     QALQDGAVGVVAPLVRSPAGNIEDSARRFPTAGVLLRKLFGARPGPDYPANRGPLAVD
                     WVAGMFMLFRSDSYRALAGFDEAYFLYYEDVDICRRMRLAGKSVIYHPGVEIVHDARR
                     ASRRNPRLALHHLASALRYFSRS"
     gene            complement(2494..>3005)
                     /locus_tag="AC158Q_00150"
     CDS             complement(2494..>3005)
                     /locus_tag="AC158Q_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338208.1"
                     /translation="RYARRFVLAQGAQAIRRCFYSGNLVGEPTAVMFRRADAARGFQE
                     GYRQLMDLEMWFHLLGKGAFACVPEPLCRIRRHAQQATLGHLAAGVVLEDKRRFFREF
                     LPGAGKDAAFGEKLLWDARMAVTVCRTLRAGHAVHPRDVEEVFFRRFFPGLIYPAASL
                     LWRLAHRGA"
BASE COUNT          469 a         1050 c         1063 g          423 t
ORIGIN      
        1 ggacgtgctt ggggatgaac aggccggccg cgttgaacgt gtagcgcttg ccctcgtcca
       61 gcggctcgag catcttctcg atgctggtgg tcccggtctt gccgttgcag aagaaaatga
      121 acttgtggcg gtggctcagt atcatggtcg ggctaacgcg gcggatgtgc cgggccgcgg
      181 accgggggcc cgggcggcct tctcccgccg cggcggccga gtcggggaca gcgcgatgtt
      241 atcaaacttc gccgcgcgcc gccgcccgcc ggccgcgatc ctcgcggcgg accgcggcgg
      301 cccgatgacc ggttccgtga gaaaatgagc ccgcgccggc gccgcgcgcc ggcgcccgct
      361 taccccagga aaaaaaccag atgaaactca gcgtcatcgg caccggctac gtcggcctgg
      421 tcaccggcgc ctgcttcgcg gagatgggca acacggtggc ctgcctcgac acggacagcg
      481 cgagggtgga ggcattgcgc gccggcaagg tgcccttcta cgagccggga ctggcggaac
      541 tcgtggcgag caacgggcgc gaggggcgcc tgcgcttcac cagttcgcac gcggaggcga
      601 tcgcggacgc gcaggtacac ttcatcgcgg tcggcacgcc gcagggcgag aacggcgcgg
      661 cggacctcac ccacgtgctg gaggcggcgc ggcagatcgg ccgccacgcg ctgcagccgt
      721 gcgtggtggt ggacaagtcc acggtcccgg tcggcacggc ggagcgggtg cgcgccgtca
      781 tcggcgagga gctggcgcgg cgccgggcgt cggtgcgctt cgaggtggtg agcaacccgg
      841 aattcctcaa ggaaggcgcc gcggtcgccg atttcatgcg ccccgagcgc gtcgtcatcg
      901 gcaccgattc ggcggaggcc gcggaaaccc tgcgccagct ctacgcgccg ttcacccgca
      961 gccacgagcg cctgctggtg atgggcgtgc gcgacgcgga gctcaccaag tacgccgcca
     1021 acgcgatgct cgcgacgcgc atctccttca tgaacgagat cgccggcctg tgcgagcgcc
     1081 tgggcgtgga cgtcgagaac gtgcggcgcg gcatcggctc ggactcgcgc atcggccacg
     1141 cgttcatcta ccccggctgc ggctacggcg gctcctgctt tccgaaggac atgacggcgt
     1201 tgatccacat ggcgaaatcg agcgggctcg cgccgggact cctgcaggcg gtggaagcgc
     1261 gcaaccagtc gcagaagcag cgtctctacg aaaagatcgc ggagcgcttc ggtgccgggc
     1321 tttcggggcg cgtcatcgcg ctgtggggcc tcgcgttcaa gccgggcacc gacgacctgc
     1381 gcgaggcgcc cgccctcgcg ctgatcgagc ggctgctcga ggcgggcgcg gcggtgcacg
     1441 cctacgaccc ggtggcgatg gacaacgcgc ggcgcagcct gccgcgcgcg tggcagcagg
     1501 cgggcaggct cgaattcgcg gcaacccagt acgacgcggt cgcgggcgcc gacgcgcttg
     1561 cgctggtgac cgagtggaag ccgttccgcc agccggattt cgattcgatg aaaaagctca
     1621 tgaagacgcc ggtcatcttc gacgggcgca accagtacga cccgggccag gcgcgcgccg
     1681 cggggttcga gtacttcggc atcggccggt gacgccggcg cggcgcccgc accgtcgaca
     1741 ggcaacgccc atctgacggc cgccgccgtg gcgcatccag ccatcaccct gtcggtcgtc
     1801 agccacggcc agaacgcgct ggtcaatcaa ctgatcgggg acgtcgcgcg ccattgcgcg
     1861 gggtcggtcg cgctggtgct cacccagaac gcgcccgacg ccgtgccgct ggcggccgcg
     1921 gaccgcctgc cgggcctcga tgtcgtggtg aacgagcgtc caaaaggcta cggcgcgaac
     1981 cacaacgcgg ccttcgcccg ctgccgcacg ccgtatttct gcgtggcgaa cccggacatc
     2041 cgcctgccgt ccgacccgtt cccgccgctg ctgcaggcgc tgcaggacgg ggccgtggga
     2101 gtcgtggcgc cgctggtgcg cagccccgca ggcaacatcg aggacagcgc gcggcgcttt
     2161 ccgacggccg gcgtcctgct gcgcaagctg ttcggcgccc ggccggggcc ggattacccg
     2221 gcgaaccgcg ggccgctcgc ggtcgactgg gtcgcgggaa tgttcatgct ctttcgcagc
     2281 gactcgtacc gggcgctcgc cggcttcgac gaggcgtact tcctctatta cgaggacgtc
     2341 gatatctgcc gccggatgcg cctggccgga aaatcggtga tctaccaccc gggcgttgaa
     2401 atcgtccacg atgcgcggcg cgcgagccgc aggaacccgc gcctcgcgct ccatcacctc
     2461 gccagcgcgc tgcgctactt ttcccggtcc tgatcaggcg ccccggtgcg ccagccgcca
     2521 caaaagggag gcggccgggt aaatcaggcc ggggaagaaa cgccggaaaa aaacttcctc
     2581 cacgtcgcgc gggtggacgg cgtggccggc gcgcagcgtg cggcagacgg ttaccgccat
     2641 gcgcgcgtcc cacagcaact tctcgccgaa cgcggcatcc ttgcccgcgc cgggcaggaa
     2701 ctcgcggaag aaacggcgct tgtcctccag cacgacgccc gctgcgagat gaccaagcgt
     2761 ggcctgctgc gcatgccgcc gtatccggca taggggctcc ggcacacagg cgaacgcgcc
     2821 tttgcccagc agatgaaacc acatctccag gtccatgagc tgccggtaac cctcttgaaa
     2881 tccccgcgcg gcgtcggcgc gccggaacat gaccgcggtg ggctcgccga cgagattgcc
     2941 ggagtagaaa cagcgccgga tcgcctgcgc cccttgcgcc agcacgaagc gccgcgcata
     3001 gcgct
//
LOCUS       DBNGSG010000009         4781 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2378995, whole genome shotgun sequence.
ACCESSION   DBNGSG010000009 DBNGSG010000000
VERSION     DBNGSG010000009.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4781)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4781)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4781
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2378995"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /locus_tag="AC158Q_00155"
     CDS             <1..951
                     /locus_tag="AC158Q_00155"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HIR3338209.1"
                     /translation="FDETLAFLAMHCEVESNQQDAALAPDALARKLADRDGVMCALTD
                     RIDAQLLERCARLKAVANIAVGYNNIDIAACTARGVMATNTPGVLDDSTADLAWALML
                     GAARRLTELERRVRGGEWKGWRLKQWLGVDVHHATLGIFGMGRIGQASARRAAGFEMQ
                     VLYHNRNRVAPEIEKRLNARHVTKDELLKQSDFVVLQVPYSPETHHLIGAPELELMKP
                     SAVLVNSTRGGVVDDAALVAALGNGTIRAAGLDVFENEPGLNPGFLALDNVVLMPHIG
                     SSTEATRRAMAMTAARNLVAALEGGTPPNLLNPGVKGNRA"
     gene            1098..4016
                     /locus_tag="AC158Q_00160"
     CDS             1098..4016
                     /locus_tag="AC158Q_00160"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR3338210.1"
                     /translation="MAGHRTGDRVHAEQVRLLYRLSLPAYVGTLINASIVVFALWGVV
                     SSTLLGAWLCVMFIVTGARFLLYRAYQGVQPPDAEANRWAWRFTVGAGIAGLLWGLAG
                     SALFPASSLPHQFLVIFLIGGMTMVAMVILAPVRQAFLAFMLPAITLLCVTVFLQGTM
                     LHLFMGALIVVYGLLLLSITPIISEMMRGTLRVKFENTALVEKLSDANRELSERVGAQ
                     QRADEGLRQSEQLYRYMFEANPLPMWIRDERTMAILAVNEATLRSYGYSREEFLQLKS
                     TDIIASEDIDRYLETMSRRDPGQTLTSQWRHRRKGGSVMDVETVSHGCELGGRQARLI
                     LVNDVTERKRGERRMRMEQTINRLLADARSVEDALPQVLRAIGESGGWLYGARWEYDK
                     AANLLRCTETWCVDSPAVKEFTAFSRTRMQAPGSGTGPIHQVWASNAPAWIADIARDG
                     GTRRAPEALKAGLRSMFAFPIMLGDAFYGVAEFFGREPGEPEPELIHFVQILGGQIGQ
                     FIARKQAEKNLQFFASHDPLTGLFNRSIFNDRLQQALAQAARFERSLALLFIDLDGFK
                     LVNDSLGHNVGDALLAELATRLRVTLREGDVIGRMGGDEFVVLIEEFAEATQVAEVAQ
                     KVLETVTRPFTLQGRECQVTASIGISIFPGDGKEAQTLMKNADIAMYQVKQRGKNSFR
                     FHSPDMNVHLMERLSLETGLRQAIQHGEFLLLYQPKVGVRDGQVSGVEALMRWQHPTQ
                     GMISPAEFVPVAEDAGLIATMGEWVLRTACRQVRVWREQGLPLLRIAVNLSPRQLAHD
                     GLIQVVREALHHAMVDPGRLELEITEEMALRNPERAARLVAQLKDLGARVEIDDFGTG
                     YSSLSFLRRMPIDSVKIDRSLILDLPRDDNAAAMTRGVVAMAHSLGIAVTAEGVETRE
                     QWEYLHQLGCEEMQGNYFCAPIAPEIVPGIVRQPSAPGRGASVQPLRPRRGEGGTEQQ
                     "
     gene            4202..4630
                     /locus_tag="AC158Q_00165"
     CDS             4202..4630
                     /locus_tag="AC158Q_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3338211.1"
                     /translation="MKTVMQLLQAKGREIHSIGPDARVFDALKLMAERNVGALVVTEG
                     GKLAGIISERDYARKVILLSKSSHDIAVREIMTAKVITVQPGQTVEDCMALMTSKRIR
                     HLPVTDGDRLVGVLSIGDLVKEVIADQEATIKQLESYIHS"
BASE COUNT          880 a         1478 c         1594 g          829 t
ORIGIN      
        1 ttcgacgaaa cgctggcctt tctcgcaatg cactgcgagg tcgaatccaa ccagcaggac
       61 gccgcgctgg ctcccgatgc gctcgcgcgg aagctcgccg accgggatgg cgtgatgtgc
      121 gcgctgaccg accgaatcga cgcgcaactc ctggaacgct gcgcccggtt gaaggcggtg
      181 gcaaacatcg cggtcggcta caacaatatc gatatcgccg cctgcaccgc gcgcggcgtg
      241 atggcgacga ataccccggg cgtgctggac gattccacgg cggacctcgc ctgggcgctc
      301 atgctcggcg cggcgcggcg cctgaccgaa ctcgagcgcc gcgtgcgcgg cggcgaatgg
      361 aagggatggc ggctgaagca gtggctgggc gtggacgtgc accacgccac gctcggcatt
      421 ttcggcatgg ggcgcatcgg gcaggcgagt gcacgccgtg ccgccggttt cgagatgcag
      481 gtgctctacc acaaccgcaa tcgggtcgcc cccgagatcg agaaacggct gaatgccagg
      541 cacgttacca aggatgaact gctgaaacag tccgacttcg tcgtgctgca ggtgccttat
      601 tcgcccgaaa cccaccatct gatcggcgcg cccgaactgg aactcatgaa accaagcgcg
      661 gttctcgtca attccacgcg cgggggtgtg gtggacgacg cggccctcgt cgctgcgctc
      721 ggcaacggca ccatccgcgc ggcgggcctc gacgtgttcg aaaacgagcc cggcctcaac
      781 ccgggttttc tcgcgctcga taacgtggtg ttgatgccgc atatcggcag ttccaccgag
      841 gcaacccgac gcgcgatggc catgactgcc gcccgcaatc tggttgccgc gctggaagga
      901 ggcacaccac ccaaccttct gaatcccggg gtgaaaggaa accgtgcctg accggggata
      961 cggcgcctgt tccccttgag ggattggtcg ggtccaagtc cgaccaatcc cttgtgcttc
     1021 gtgtcacaat tccagattcc acgacggtgc cgggccgtca acaaccataa cccggaaatc
     1081 ccgctcagga cagggaaatg gccgggcata gaacagggga ccgcgtgcac gccgagcagg
     1141 tgcgcctgct ctataggctg tcgctgcccg cgtacgtcgg caccctgatc aacgccagca
     1201 tcgttgtctt cgcgctgtgg ggcgtggtgt cctccaccct gctgggcgcg tggctgtgcg
     1261 tcatgttcat cgtcacgggc gcgcgctttc tgctctatcg cgcctaccag ggcgtccagc
     1321 cgccggacgc cgaggcgaat cgctgggcgt ggcgcttcac cgtcggcgcc ggcatcgccg
     1381 gcctgctgtg gggactggcc ggctcggcgc tgttcccggc gtcttcgctg ccacaccagt
     1441 tcctggtgat cttcctcatc ggcggcatga cgatggtggc aatggtgatt ctcgccccgg
     1501 tgcggcaggc atttcttgcc ttcatgcttc ccgccattac cctcttgtgc gtgactgtgt
     1561 tcctgcaagg cacgatgctg cacctgttca tgggcgcgct gatcgtcgtg tacgggctgc
     1621 tcctattgag catcactccc atcatttccg aaatgatgcg cggcacgctg cgggtgaagt
     1681 tcgagaatac cgcgctggtc gagaaactgt cggatgccaa ccgcgagctg tccgagcgcg
     1741 ttggcgcgca gcagcgcgcc gatgaaggtt tgcgccagag cgagcaactc taccgctaca
     1801 tgttcgaggc caacccgctg ccgatgtgga tacgggacga acgcacgatg gccatcctgg
     1861 cggtgaacga ggcgacgctg cgaagctacg gctattccag ggaggagttc ctgcagctga
     1921 aatcaactga catcatcgcg agcgaggaca tcgaccgcta tctcgaaacg atgagccggc
     1981 gcgatcccgg gcagaccctg acgagccaat ggcgccatcg ccgcaagggc ggctccgtta
     2041 tggatgtcga gaccgtatcc cacggctgcg aactcggcgg gcgccaggcg cgcctgattc
     2101 tcgtgaacga tgttaccgag cgcaagcgtg gtgagcggcg catgcgaatg gaacagacga
     2161 tcaaccggct gctcgcggat gcgcgctccg tggaggacgc gctgccgcag gtattgcgcg
     2221 ccatcggaga atccggtggc tggctgtacg gggcgcgatg ggagtatgac aaggcggcca
     2281 acctgctgcg ttgcacggag acctggtgcg tggattctcc ggccgtgaag gaattcacgg
     2341 ctttctcccg gacccgcatg caggcacccg ggtccggaac gggccccatt catcaggtgt
     2401 gggccagcaa tgcccccgcc tggatagcgg atattgcccg cgacggcggc acgcggcggg
     2461 cgccggaagc gctcaaggcc gggctgcgct cgatgttcgc ttttccgatc atgctgggcg
     2521 atgccttcta cggcgtcgcg gagttcttcg ggcgcgagcc gggcgagccc gagccggagc
     2581 tgatccattt cgtgcaaatt ctgggcggcc agatcgggca gttcatcgcc cgcaagcagg
     2641 cggaaaaaaa cctgcagttc tttgccagcc acgacccgct caccgggctc ttcaatcgca
     2701 gcatattcaa cgaccgcctg cagcaggcgc tggcgcaggc ggcccggttc gagcgcagcc
     2761 tggcgttgct attcatcgat ctcgacggct tcaagctcgt caacgactcg ctgggtcaca
     2821 atgtgggaga cgccctgctc gccgaactgg cgacgagact gcgcgtcacc ctgcgcgaag
     2881 gcgacgtgat cggccgcatg ggcggcgacg agttcgtggt gctgatagag gagttcgcgg
     2941 aagcaaccca ggtcgcggaa gtcgcacaaa aagtgctgga gaccgtgaca cgtccgttca
     3001 cgctgcaggg gcgggaatgc caggtgacgg caagcatcgg catcagtatc ttccccggcg
     3061 atggcaagga agcgcagacg ctaatgaaga atgccgacat cgcaatgtac caggtcaagc
     3121 agcggggcaa gaacagcttc cgcttccatt cgcccgacat gaacgtgcac ctgatggaac
     3181 ggctatcgct ggagacggga ctgcggcagg ccatccagca cggcgaattc ctgctgttgt
     3241 accaacccaa ggtcggcgtc cgtgacgggc aggtgagcgg ggtggaggcc ctgatgcgct
     3301 ggcagcaccc gacacagggc atgatcagcc ctgcggaatt cgtgccggtg gcggaggatg
     3361 cggggctgat cgcgacaatg ggcgaatggg tgctgcgcac ggcatgccgg caggtgcgtg
     3421 tctggcgcga gcagggcctg ccgttgctgc ggatcgctgt caacctgtcc cccaggcagc
     3481 tggcacacga cggcctgatc caggtggtgc gcgaagcgct gcaccacgcg atggtggatc
     3541 cggggcggct cgagctcgag attaccgagg agatggcgct ccgcaacccg gagcgggcgg
     3601 ccaggctcgt cgcgcagttg aaggatctcg gggcgagagt ggagatcgac gacttcggca
     3661 ccggctattc ctcgctgagc tttctcaggc ggatgccgat cgacagcgtc aagatcgacc
     3721 gctccctgat actcgacctg cctcgcgacg acaatgccgc ggcgatgacg cgcggtgtgg
     3781 tcgcgatggc gcacagcctc ggcatcgcgg tgaccgccga aggggtggaa acccgcgagc
     3841 agtgggaata cctgcaccag ctgggatgcg aggagatgca gggtaattac ttctgtgcgc
     3901 cgattgcacc cgaaatcgtg ccgggcatcg tgcggcagcc ttcggcgccc ggccggggtg
     3961 ccagcgtgca gccattgcgc ccgcggcgcg gcgaaggcgg caccgagcag caataaccgg
     4021 ccgaccgccg tctctccgcc agccgccgtt cgcgcatcga cccggaacca gggcgccccg
     4081 cccgccgccg atcttggcga gaaggcctcg taatcgcgcg gtgctttatg gcgcattcga
     4141 atttggctac actggtttgc ctaggcaggt atcgggacgg cgcaagccgg aggagatggc
     4201 catgaagacc gtgatgcagc tgttgcaggc gaagggcagg gagatccatt cgattggccc
     4261 ggacgctcgt gtattcgacg cgttgaagct gatggcggaa aggaatgtcg gcgcgcttgt
     4321 cgtgacggaa ggtggaaagc ttgcgggcat catctcggag cgcgactacg cgcgcaaggt
     4381 gatcctgctc agcaagtcct cgcacgacat cgcagtgcgc gagatcatga ccgcgaaggt
     4441 gatcaccgtt cagcccggcc agaccgtgga ggactgcatg gcactgatga cttcgaagcg
     4501 catccggcac ctgccggtga ccgacggcga ccggctggtt ggcgtgctct cgatcggcga
     4561 cctggtgaag gaagtgatcg ccgaccagga agcgaccatc aagcagctcg agagctacat
     4621 acacagctga accgtcaaga cgccaagtcc gccaagaaaa tccaggcgac agatttaatg
     4681 gcctgataag cacgcgccgg caagtgcagt agacggcgca ggcccttttt ttgattatta
     4741 ttattgtatt tcttggcggc cttggcgtct tggcggttaa t
//
LOCUS       DBNGSG010000010         6036 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3398085, whole genome shotgun sequence.
ACCESSION   DBNGSG010000010 DBNGSG010000000
VERSION     DBNGSG010000010.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6036)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6036)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6036
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3398085"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1866
                     /gene="rpoB"
                     /locus_tag="AC158Q_00170"
     CDS             <1..1866
                     /gene="rpoB"
                     /locus_tag="AC158Q_00170"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006029840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIR3338212.1"
                     /translation="VRVNDEETAPGEVGVDIYNLVKYQRSNQNTNINQSPLVKVGDRL
                     AKGDVVADGASTDMGELALGQNMLVAFMPWNGYNFEDSILISERVVADERFTSIHIEE
                     LSVVARDTKLGPEEITRDISNLSENQLANLDESGIIAIGAEVEAGDVLVGKVTPKGET
                     QLTPEEKLLRAIFGEKASDVKDTSLRVPSGMSGTVIDVQVFTREGIERDKRAEQIIDD
                     ELKRYKKDLADQLRIVENDAFERLERLLIGKTANGGPKKLARGAKISKAYLTDVERHG
                     WFDIRVANDKVAAQLESIKESLAQTKALFDKAYEEKKKKLTQGDELPPGVQKMVKVYL
                     AVKRRLQPGDKMAGRHGNKGVISKITPVEDMPYMADGTPVDIVLNPLGVPSRMNVGQI
                     LETHLGWAAKGLGLKIGEMIKSQAKAAEIRKLLNRIYDNGGRNEDLGSLGDEDVMQLA
                     GNLREGVPFATPVFDGATEKEIKEMLDLAGLPRSGQVTLHDGRTGDPFERPVTVGYMH
                     VLKLHHLVDDKMHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQE
                     MLTVKSDDTEGRRKVYESIVKGDHRIEAGMPESFNVLVKEIRSLAIDIDLDHNRY"
     gene            1945..6036
                     /gene="rpoC"
                     /locus_tag="AC158Q_00175"
                     /pseudo
     CDS             1945..6036
                     /gene="rpoC"
                     /locus_tag="AC158Q_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081256.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
BASE COUNT         1295 a         1793 c         2000 g          948 t
ORIGIN      
        1 gttcgcgtga acgacgagga aaccgcgccg ggcgaggtag gcgtggacat ctacaacctg
       61 gtcaagtacc agcgctccaa ccagaacacc aacatcaacc agagtccgct ggtcaaggtg
      121 ggtgaccgcc tcgccaaggg cgacgtggtg gcggacggcg cgtctaccga catgggcgaa
      181 ctcgcgctcg ggcagaacat gctggtcgcg ttcatgccgt ggaacggtta taacttcgag
      241 gactcgattt tgatttcgga gcgtgttgtc gcggacgagc gcttcacttc gattcacatc
      301 gaggaactct cggttgtggc acgcgacacc aagctgggac ccgaggaaat cacgcgggat
      361 atctcgaacc tgtccgagaa ccagctcgcc aatctcgacg agtcggggat catcgcaatc
      421 ggcgcggaag tcgaagccgg agatgtgctg gtcggcaagg tcacgccgaa aggcgagacg
      481 cagttgacgc ccgaagaaaa actgttgcgc gcgatcttcg gtgagaaggc ttccgacgtg
      541 aaggacacct cgctgcgcgt gccgtccggc atgtccggca ccgtgatcga cgtccaggtg
      601 ttcacccgtg aaggcattga gcgcgacaag cgcgccgaac agatcatcga cgacgagctg
      661 aagcgctaca agaaagatct cgcggaccag ctgcgcatcg tcgagaacga cgctttcgaa
      721 cgtctcgagc ggctgctgat cggcaagacc gccaacggcg gcccgaagaa actcgccagg
      781 ggcgccaaga tcagcaaggc ttatctcacc gacgtcgagc gccacggctg gttcgacatc
      841 cgtgtcgcga acgacaaggt cgcggcgcag ctggagagca tcaaggaaag cctggcgcag
      901 accaaggcgc tgttcgacaa agcctatgag gagaagaaaa agaagctcac ccagggcgac
      961 gagctgcccc cgggcgtgca gaagatggta aaggtctatc tggccgtcaa gcgccggctg
     1021 cagccgggcg acaagatggc gggccgccat ggcaacaagg gcgtgatctc caagatcacg
     1081 ccggtggagg atatgccgta catggcggat ggcacgccgg tggacattgt gctgaacccg
     1141 ctgggcgtgc cgtcacgcat gaacgtggga cagatcctgg agacccacct gggctgggcg
     1201 gccaaggggc tgggcctgaa aatcggcgag atgatcaagt cgcaggcaaa ggcggcggag
     1261 atacgcaagc tgctcaaccg catctacgac aacggcggca gaaacgagga cctgggctcg
     1321 ctcggcgacg aggatgtcat gcaactggcc gggaacctca gggaaggcgt gccgttcgcg
     1381 acgccggtgt tcgatggcgc caccgagaag gaaatcaagg agatgctcga cttggcaggc
     1441 ctgccgcgca gcggccaggt gacgctccat gacgggcgca cgggggaccc cttcgagcgg
     1501 ccggtaacgg tgggttacat gcacgtgctg aaactgcacc atctggtgga cgacaagatg
     1561 cacgcccgct cgaccggacc gtactccctt gtcacccagc agccgctggg cggcaaggcg
     1621 caattcggcg gccagcgctt cggcgagatg gaagtgtggg cgctggaagc ttacggcgcc
     1681 gcctacacgc tgcaggagat gctgaccgtg aagtccgacg acacggaagg gcggcgcaag
     1741 gtctatgagt ccatcgtcaa gggtgaccac cgcatcgagg ccggcatgcc ggaatcgttc
     1801 aacgtgctgg tgaaggaaat ccgctcgctg gcgatcgaca tcgacctcga ccacaaccgt
     1861 tattgattca gtgaggcgca agggcagcac cgaagctcaa acccgaagcc caaccccacg
     1921 gtctcgctta acaaggaacg cgaaatgaaa gcactgctcg atttgttcaa gcaggtcacg
     1981 cagcaagagg aattcgacgc gatcaagatc gggcttgcct cgcccgagaa aatccgctcg
     2041 tggtcgtacg gcgaagtcaa gaagccggag acgatcaatt accggacttt caagccggag
     2101 cgcgacgggc tgttctgcgc caagatcttc gggccggtca aggattacga gtgcttgtgc
     2161 ggcaagtaca agcggctgaa gcaccgcggc gtgatctgcg agaagtgcgg cgtcgaggtg
     2221 acgctgtcca aggtgcggcg cgagcgcatg ggccacatcg agctcgcgag cccggtggcg
     2281 cacatctggt tcctgaaatc gctgccgtcc cgcatgggca tggtgctcga catgacgctg
     2341 cgcgatatcg agcgcgcgct ctatttcgag gcatacgtcg tgatcgatcc gggcatgacg
     2401 ccgctgaagc gcgcgcagct catgaccgag gatgattacc tcgccaaggt cgaggaattc
     2461 ggcgacgact tcaccgcgat gatgggcgcg gaaggcatcc gcgagcttct ccgcagcctg
     2521 gacctcaaga aggaaatcga gaccctgcgc gaggacctgg cggccacgac ctcggatacc
     2581 aagatcaaga aaatcgcgaa gcgtttgaag gtgctggaag cgtttcacaa gtccggcatc
     2641 aagccgggct ggatgatcct cgaagtgctg ccggtgctgc cgccggaact gcggccgctg
     2701 gtgccgctcg acggcggtcg cttcgccact tcggacttga acgatcttta tcgccgcgtg
     2761 atcaaccgca acaaccggtt gaagcgcctg ctcgagctga aagcgcccga gatcatcgtg
     2821 aggaacgaga agcgcatgct gcaggaagcg gtggactcac tgctcgacaa cgggcgccgc
     2881 gggaaggcga tgaccggcgc caacaagcgg ccgctgaagt cgctcgccga catgatcaag
     2941 ggcaagagcg gccgcttccg ccagaacctg ctgggcaagc gcgtggacta ctccggacgc
     3001 tccgtgatcg tggtgggccc gcagctgaag ctgcaccagt gcggcctgcc caagctgatg
     3061 gcgctggaac tgttcaagcc ctacatcttc cacaagctgg aagtgatggg attggccacg
     3121 accatcaagg ccgccaagcg catggtggaa gaccaggaag ccatcgtgtg ggacatcctg
     3181 gaagaggtga tccgggaaca cccggtgatg ctgaaccgcg cgccgacact gcatcgtctc
     3241 ggcatccagg cgttcgaacc ggtgctgatc gagggcaagg cgatccagct gcacccgctg
     3301 gtgtgcgcgg cgttcaacgc cgacttcgac ggcgaccaga tggcggtgca cgtgccgctg
     3361 tcactggaag cgcagatgga ggcgaggacg ctcatgctct cctccaacaa catcctgtcg
     3421 cccgcgaacg gcgagccgat tatcgtgccc tcgcaggaca tcgtgctcgg cctctactac
     3481 gccacgcgcg agaaggtggg cgcgcgcggt gaaggctcgc tcttcaccaa tctttcagaa
     3541 gtttcgcgcg cgcacgacac cggccaggtc gaagtcaatg cccgcatcac ggtgcgtatc
     3601 aaggagcaga ccgttgacgc ccacggcgag aagcgcgaga agctgacgcg ctacgggacc
     3661 accgtcggac gcgcactgct gtccgagata ctcccgcccg gcctgtcttt cgaactcatc
     3721 aacaaggcgt tgaagaagaa ggagatttct cgtctgatca acgcgagctt ccgccgttgc
     3781 ggcttgcggg aaaccgtgat cttcgccgac aagttgatgt atgccggatt tgccatggcg
     3841 accaaggcgg gcctttccat cgcggtggac gacatgctga tccccactca aaagggcgag
     3901 atcatctctg acgccgagaa ggaggttcag gagatcgaga agcagtacac ctcgggcctg
     3961 gtgacccagg gcgagcgcta caacaaggtg gtcgacatct ggggccgcgc cggcgatctc
     4021 gtcgcgaagg caatgatgga acagctcggt gtcgagccgg tagtggactg ggacgccaag
     4081 aagcacgaat gggtgcagcg caaggacaag aaaggcgcgc cggtgatgca ggagtcgctg
     4141 aactccatct acatgatggc ggactccggt gcgcgcggtt ccgcggcgca gatccggcag
     4201 ctcgccggca tgcgcgggtt gatggcgaaa ccggacggct cgatcatcga gactccgatc
     4261 accgcgaact ttcgcgaggg cttgaacgtg ctgcaatact tcatctccac gcacggcgcg
     4321 cgcaaaggcc tggcggatac ggcgctgaag acggcaaact cgggctatct cacccggcgt
     4381 ctggtggacg tgacgcagga tcttgtcgtc accgaggacg actgcggcac cacccagggc
     4441 gtctcgatga aggcgctggt cgaaggcggt gaagtggtcg aaagcctgcg cgagcgcatc
     4501 ctcggccgcg tgtgcgcgac cgatctcgtt aatcccgaga gcggcaaggt aatgtgtcct
     4561 gtaaataccc tgctcgacga ggacgtggtg gatgcgatcg aggccgcggg cattgacgag
     4621 gtcaaggtgc gcacaccgct ctcctgcgac acacgctacg gcctgtgcgc caagtgctac
     4681 gggcgcgacc tgggccgcgg cacgccgatc aacgtcggcg aggcggtcgg cgtgatcgcc
     4741 gcgcagtcga tcggcgagcc gggcactcag ctcaccatgc gcaccttcca catcggagga
     4801 gccgcttcgc ggaccgcggt ggtcagccag atcgagagca agtcgaacgg cgtggtgcgt
     4861 tatagcgcgg gaattcgtta cgttacccac tctcgcggcg aactcgtcgc gatctcgcgc
     4921 aacggcgagg tggtgataca tgatgaatcc ggccgcgagc gcgagcgcca caaggtaccg
     4981 tacggcgcga cgctacttgc gcgcgatggc gaggcggtca aggcggggca cgtgctggcg
     5041 acgtgggatc cgcacacccg tcccatcatc accgagtacg ccgggcgcat caagttcgag
     5101 aacgtcgagg aaggctcgac cgtcatgaag caggtcgacg aagtgaccgg cctgtcgacc
     5161 ctcgtggtcg tggatcccaa gcgccgcggc agcgtacagg cgaagggcct gcgcccgcag
     5221 gtgaagtttc tcgacgaatc cggcaacgag atcaagatgg cgtgatccta tcagtcggtc
     5281 aacatcacgt tccagatcgg ctgcatcatc acggtgcgtg acggccagga cgtgggcgtg
     5341 ggcgaagtgc ttgcgcgcat tccgcaggaa acttcgaaga cgcgcgacat taccggcggc
     5401 ctcccgcgag tggcggagct gttcgaggcg cgctcgccga aggatgcggg ggtgctcgcg
     5461 gaagtcaccg ggacggtgtc gttcggcaag gacacgaagg gcaagcagcg gctggtcatt
     5521 accgatcccg atggcgtcgc gcatgagtac ctgatctcga aagacaagca cgtcatggct
     5581 cacgacggcc aggtcgtgaa caagggagag atcatcgtcg acggcccggc cgatccccac
     5641 gacatcctgc ggctgaaagg cgtcgaggag cttgcacgct acatctgcaa tgaagtgcag
     5701 gacgtctacc ggctgcaggg cgtgaagatc aacgacaagc acatcgaggt aatcgtgcgc
     5761 cagatgctgc ggcgcgtaac catcgcggat cccggcgata cccggttcat caccggcgag
     5821 cagctcgagc gcgccgaggt gctggaagag aacgagaaag tggaggccga gggcaagaag
     5881 ccggcgacct acgagtacat gctgctcggc atcaccaagg cctcgctttc cacggactcg
     5941 ttcatttccg ccgcgtcctt ccaggaaacc acgcgcgtgc tcacggaagc agccatcatg
     6001 ggcaagcgcg acgagctgcg gggcttgaag gagaac
//
LOCUS       DBNGSG010000011         5288 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3568363, whole genome shotgun sequence.
ACCESSION   DBNGSG010000011 DBNGSG010000000
VERSION     DBNGSG010000011.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5288)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5288)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5288
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3568363"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="AC158Q_00180"
     CDS             <1..854
                     /locus_tag="AC158Q_00180"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR3338213.1"
                     /translation="QKVISQIDADGNATVLLNRPEVHNAFDPEMVDALTAALKKLEAD
                     DKVRAIVITGSGRNFCAGADIEHMKRSANFSRAQNLDNARATAMMLRTLYALEKPTIA
                     CVRGAARGGGVGLVSACDIAIAERGATFLLSEVKLGIVPAMISPFVIGGIGRRHAHRY
                     MLSGEAFDSAEAYRIGLLHDICEEPELNAVVGRMLANLHSSGPNAIVAIKKLIPRVAA
                     ARVDEALMETLSERIAQIRTTAEAQEGLSAFLEKRKTAWSTPASKRGKKKPENSRQDA
                     KAAKKDK"
     gene            1002..1922
                     /locus_tag="AC158Q_00185"
     CDS             1002..1922
                     /locus_tag="AC158Q_00185"
                     /EC_number="4.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004419 - hydroxymethylglutaryl-CoA lyase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HIR3338214.1"
                     /translation="MTLPRKVKLVEVGPRDGLQNESAPVSTEVKVELIHRLQDAGLKV
                     IEATAFVSPRWVPQMADNAQVMAAIRRKPGVSYPVLVPNRKGLEGALAAKAGEVVVFG
                     AATESFSKKNTNCTIAEGLARFAEVAKEALAHGLKVRGDISVCLGCPYEGEVRPDAVV
                     RVARELDAMGCYEITIADTIGTGTAGKTRAVFEAVMEHIPAARLAGHFHDTYGQAVSN
                     VYAALECGVPTFDSSVAGLGGCPYAKGATGNVATEDVLYLLDGLGIETGVDMRALIAA
                     GEYICRQLGRPTNSRAAKALSAKAAAKAAA"
     gene            1922..2119
                     /locus_tag="AC158Q_00190"
     CDS             1922..2119
                     /locus_tag="AC158Q_00190"
                     /inference="COORDINATES: protein motif:HMM:NF018630.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HIR3338215.1"
                     /translation="MEEAAIESPCIRECRVDQVTGYCVSCFRTLTEISYWESYTPREQ
                     QRVMALIQARRAEAAARTVEH"
     gene            2139..2693
                     /locus_tag="AC158Q_00195"
     CDS             2139..2693
                     /locus_tag="AC158Q_00195"
                     /inference="COORDINATES: protein motif:HMM:NF022586.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HIR3338216.1"
                     /translation="MPLPNPAPRKLMHTRAIECKGYEREDGLWDIEARLTDTKTVMHT
                     RHHGGRERSPGEPVHDMWVRLTIDLDMKVHDVEAVTDEGPYPHCGHITPNFKALAGLT
                     IGPGWRKGIAERVGGVKGCTHLVELMGPLGTTAFQATNRARLARSAGKPATKKPYQLN
                     SCHVYKDDSPAVLERWPQFYTGPR"
     gene            2748..3143
                     /locus_tag="AC158Q_00200"
     CDS             2748..3143
                     /locus_tag="AC158Q_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338217.1"
                     /translation="MLRKIWRLVEILLMLLGLAAIPVIAMQVRDHGMRKVVEPPWSVQ
                     TLDEFRKWRPQYGNAFRIETRGSAYYLVHGERARALASGPSGYVFDGRGNFVGWIRDT
                     GDDSYLRIVMNEAGRKPIPVSAIPNSVTR"
     gene            complement(3212..3616)
                     /locus_tag="AC158Q_00205"
     CDS             complement(3212..3616)
                     /locus_tag="AC158Q_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338218.1"
                     /translation="MELSEGIRKLGFQRWYERQLLESHFYLVTAFLCLVLVMACLEGF
                     RFHAPGLEPLLRLTVMFAGGAIGVASLNRYFRMLRLALHAANRSVCGKCGTHGILEVT
                     NVRAGARDGEADTICAPTGVRCRKCGHEWTME"
     gene            complement(3775..5004)
                     /locus_tag="AC158Q_00210"
     CDS             complement(3775..5004)
                     /locus_tag="AC158Q_00210"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015112 - nitrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3338219.1"
                     /translation="MSAARLAPVQLTLLMCVAEVLCMTGFATYTTLLPALQKEWGLSN
                     SEAGLISGIYYAGYIAATPVLTSLTDRVDARRIYAFACALSVAGAAGFVLFSQGLWSA
                     LFFQFLIGAGLGGSYMPGLKMLTDQLEGAVQSRALAFYTASFGIGSTFSIFACGWIGG
                     QYGWQTAFAYGATGPVLAVLLVILLMPRGRTHAAGQPAPALLDFRPVLKNRKTLPYVI
                     GYSAHNYELFGQRSWMVAFLVFTASLQPADAPMLIGAATLAAAINMLGPVMSVSGNEL
                     AIRFGRTRVIFTFMTASGLVACVLGHTATLPWVMVFLIMCLHYGLMLGDSAALTSGAV
                     ASAPPDARGATMAVYSFVGFSTACLAPLVFGMVLDFAGGNRSVFAWGLAFASIGIFGV
                     LSPLARWLYLRRYATQD"
BASE COUNT         1049 a         1735 c         1685 g          819 t
ORIGIN      
        1 gccagaaagt catctcccag atcgacgccg acggtaacgc gacggtgctg ctgaaccgtc
       61 cggaagtcca caatgcgttc gaccccgaga tggtggacgc cctgacggcg gcgctgaaga
      121 aactcgaggc ggacgacaag gtgcgcgcga tcgtgatcac cggctcgggc cgcaacttct
      181 gcgccggcgc cgacatcgag cacatgaagc ggagcgcgaa cttctcgcgc gcgcagaacc
      241 tcgataacgc gcgcgccacc gccatgatgc tgcggacgct ctacgcgctc gagaagccga
      301 ccattgcctg cgtgcgcggc gcggcgcgcg gcggcggcgt cggcctggtt tcggcgtgcg
      361 acatcgcgat cgcggagcgc ggcgcgactt tcctcctctc ggaagtgaag ctcggcatcg
      421 tcccggccat gatcagcccg ttcgtgatcg gcgggatcgg ccggcgccac gcgcaccgct
      481 acatgctctc gggcgaggcg ttcgactcgg ccgaggccta ccgcatcggc ctgctgcacg
      541 atatctgcga ggagcccgag ttgaacgccg tcgtcgggcg catgctcgcg aacctccatt
      601 cgagcgggcc caatgcgatc gtggcgatca agaaactgat cccccgggtc gccgccgccc
      661 gcgtcgacga ggcgctcatg gagacgctct cggagcgcat cgcgcaaatc cgcacgaccg
      721 cggaggcgca ggagggcctg tccgccttcc tcgagaagcg caagaccgcg tggtccacgc
      781 ccgcctccaa acggggcaag aaaaaaccgg aaaatagccg ccaagacgcc aaggccgcca
      841 agaaagacaa ataaaattag aaactacctc caacaacctt gccatgacgt aactttgatt
      901 tgcacttggt aattaaataa ccgtgctgaa aattaatcta ttgttcaagt ttttcttggc
      961 ggccttggcg tcttggcggt taatttaaaa cggtctttca catgacattg ccccgcaaag
     1021 taaaactggt ggaagtcggt ccgcgcgacg gtctgcagaa cgaatccgcg ccggtttcga
     1081 ccgaggtcaa ggtcgagttg atccaccggc tgcaggatgc cggactcaag gtgatcgagg
     1141 cgaccgcgtt cgtttctccc aggtgggtgc cgcagatggc cgataacgcg caggtgatgg
     1201 cggccatccg ccgcaagccg ggcgtgtcct atccggtact ggtgccgaac cgcaaaggcc
     1261 tcgagggagc gctcgccgcg aaagccgggg aagtcgtcgt gttcggcgcc gccaccgaga
     1321 gcttctcgaa aaagaatacc aactgcacga tcgccgaggg tctcgcgcgc ttcgccgagg
     1381 tggcgaagga agccctcgcg cacgggctca aggtgcgcgg cgatatctcg gtctgcctcg
     1441 gctgcccgta cgagggcgag gtccggcccg acgcggtggt tcgcgtcgcg cgcgagttgg
     1501 acgccatggg ctgctacgaa atcacgatcg ccgataccat cggtaccggc acggcgggca
     1561 agacccgcgc cgtgttcgag gcggtcatgg agcacattcc cgcggcgcgc ctcgccgggc
     1621 atttccacga cacctacggc caggcggtca gcaacgtcta cgccgcgctc gagtgcggcg
     1681 tgcccacctt cgattcctcg gttgcgggcc tcggcggctg tccctacgcg aagggcgcca
     1741 ccggcaacgt cgcgaccgag gacgtgctct acctcctcga cggcctcggc atcgagaccg
     1801 gggtggacat gcgcgccctc atcgccgcgg gcgagtacat ctgccgccaa ctcggccggc
     1861 ccaccaactc gcgggcggcg aaagcgctct cggcaaaagc ggcggcgaag gcggccgcgt
     1921 gatggaagaa gcggcgatcg aatcaccgtg catcagggaa tgccgcgttg accaggtcac
     1981 gggttattgc gtcagttgtt tccgtactct cacggaaatc tcgtactggg aaagctacac
     2041 gcccagggag cagcaacgcg tgatggcgct gatccaggca cgccgcgccg aggcagccgc
     2101 ccgcactgtc gagcattgaa ctactggagc caccgtccat gccgctgccg aatcccgccc
     2161 cccgcaagtt gatgcatacc cgcgcgatcg agtgcaaggg ctacgagcgc gaggacggcc
     2221 tgtgggacat cgaggcgcgt ctcaccgaca ccaagaccgt gatgcacacg cgccatcacg
     2281 gcgggcgcga gcgcagcccc ggcgagccgg tgcacgacat gtgggtgcgt ctcaccatcg
     2341 acctcgacat gaaggtccac gacgtcgagg cggtaaccga cgagggtcct taccctcatt
     2401 gcggccacat cacgcccaat ttcaaggcgc tcgccggcct caccatcggc cccggctgga
     2461 ggaagggcat cgccgagcgc gtcggcggcg tcaagggctg cacccatctg gtcgagctga
     2521 tgggcccgct cggcaccacc gcgttccagg ctaccaaccg ggcgcgtctc gcccgcagcg
     2581 ccggcaagcc ggcgacgaaa aagccctacc agttgaattc ctgccacgtc tacaaggacg
     2641 actcgcccgc cgtgctcgag cgctggccgc agttctacac cggcccacgc tagttccgcg
     2701 gcggccgcaa caggcgggat ccccgccgca acgcccggga ggcggtcatg cttcggaaaa
     2761 tctggcgtct ggtcgaaata ctgctgatgc tgttgggcct ggccgcgatt ccggtgatcg
     2821 cgatgcaggt gcgggatcac gggatgcgca aggtggtcga accgccctgg agcgtgcaaa
     2881 cgctggatga gttccggaaa tggcgaccgc aatacggaaa cgcgttcagg atcgagacgc
     2941 ggggatcagc ctactacctg gtgcacggcg agcgggcgcg ggcgctggct tccggcccga
     3001 gcggttacgt cttcgacggg cgcggcaact tcgtgggctg gatccgcgac accggggatg
     3061 attcgtacct gcggatcgtc atgaacgagg cgggcaggaa gccgatcccc gtgtccgcga
     3121 tcccgaattc cgtcactcgc tgacagcgcc tcggcctccc tgccccctcc tcactaatcc
     3181 ctcattactc atcattcatc actcatcact gttattccat ggtccactca tgcccgcact
     3241 tgcggcagcg cacgccggtc ggcgcgcaaa tcgtatccgc ttcgccatca cgggcgcctg
     3301 cgcgcacgtt ggtcacttcc aggatgccgt gggtcccgca ttttccgcag accgagcgat
     3361 tggcggcatg caacgccagg cggagcatcc taaaataacg gttcagcgac gccacgccaa
     3421 ttgcgccgcc cgcgaacatc actgtgagcc ggagcaaggg ttccaggccc ggcgcgtgga
     3481 agcggaaccc ctccaggcac gccattacga ggaccaggca aagaaaggcc gtcaccaggt
     3541 agaagtggct ctccaggagc tgccgctcgt accagcgctg gaagccgagc ttgcggatgc
     3601 cctcggatag ctccataggg agggatgagg gatgagggat gagggatgag ggatgaagga
     3661 aaacacctct gcaccgccaa ttcttccccg gaagcttacg cctgcagccg ttccccttcc
     3721 aggacgactt ggcgccttgg cgtcccttcg acatgctcag gacaggcttg gcggctaatc
     3781 ttgtgtcgcg tagcgacgca gatagagcca gcgcgccagc ggcgagagca cgccgaagat
     3841 gccgatgctg gcgaacgcga gcccccaggc gaacacgctg cggttgccgc cggcgaaatc
     3901 gagcaccatg ccgaacacca ggggcgccag gcaggccgtg ctgaaaccca cgaaggaata
     3961 gaccgccatc gtcgcgccgc gcgcatcggg cggcgcggag gcgaccgcgc cggacgtgag
     4021 cgcggcggag tctcccagca tgaggccgta atgcaggcac atgatgagaa acaccatcac
     4081 ccacggcaac gtggcggtgt ggcccagcac gcaggcaacg aggccggagg cagtcatgaa
     4141 agtgaaaatc acgcgcgtgc ggccgaagcg gatggcgagc tcgttgcccg acacgctcat
     4201 caccgggccc agcatgttga tggccgcggc gagcgtcgcc gcaccgatca gcatgggcgc
     4261 atccgccggc tgcagggacg cggtgaagac gagaaacgcc accatccacg agcgctggcc
     4321 gaacagctcg tagttgtgcg ccgaatagcc gatgacgtaa ggcagcgtct tccggttctt
     4381 cagcaccggc cggaaatcga gcagcgcagg cgccggctga cccgcggcat gggtgcgccc
     4441 acgcggcatg aggagaatga ccagcagtac cgccagaacg ggtcccgtcg cgccgtaggc
     4501 gaacgcggtc tgccacccgt attgccctcc gatccagccg caggcaaaga tcgagaaagt
     4561 cgagccgatg ccgaagctcg ccgtgtagaa agcgagggcg cgcgactgca ccgcgccttc
     4621 gagctgatcg gtcagcatct tcaaccccgg catgtagctg ccgcccaatc cggcgccgat
     4681 caggaactga aagaagagcg cgctccacaa tccctgcgag aacagcacga agccggccgc
     4741 gccggcgacc gacagcgcgc aggcgaaagc atagatccgg cgcgcatcca cgcgatcggt
     4801 gaggctggtg aggaccgggg tcgccgcaat gtagccggca tagtagatgc cgctgatcag
     4861 gccggcttcg gagttggaca agccccactc cttctgcagc gccggcagca gcgtggtgta
     4921 ggtggcgaag cccgtcatgc atagcacctc cgccacgcac atcagcagag tgagctggac
     4981 cggcgcgagc cgcgcggcgc tcatctatcg gcccggaacc agatcagcca cagatgaacg
     5041 cagataaacc cagatgaaat caagaagacc gcccaataaa actgaagaga aaaagaaccc
     5101 ttcctgttta ttccatgcct ggcttcagtt cgtccggtgc aatccgattc tatcggcgtg
     5161 catcggcgtt catcggcggt ttcaatgctt ttctcaaatc gcgcccgctt tcttcaacag
     5221 ctccgcgaac gcctccgcca tccggcggta acctatggca ttgggatgga tcgggtcgga
     5281 tttctggt
//
LOCUS       DBNGSG010000012         4297 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_173237, whole genome shotgun sequence.
ACCESSION   DBNGSG010000012 DBNGSG010000000
VERSION     DBNGSG010000012.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4297)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4297)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4297
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_173237"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="AC158Q_00215"
     CDS             <1..398
                     /locus_tag="AC158Q_00215"
                     /inference="COORDINATES: protein motif:HMM:NF025135.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4166 domain-containing protein"
                     /protein_id="HIR3338220.1"
                     /translation="GRFLCWLLNLPKAASDAELVFELDASATNEIWLRHFPGRTMRST
                     MQIADGLLVELIGFIRLWFELSVESERLVMTLARISVFGLPWPGALMPEVWGGERGNA
                     GLLHFEAGARLPAIGLLTSYNGFLVINPQ"
     gene            421..1497
                     /locus_tag="AC158Q_00220"
     CDS             421..1497
                     /locus_tag="AC158Q_00220"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015400.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HIR3338221.1"
                     /translation="MRVVVLGGFGNFGARICRALANDPGIEVVPAGRTDRGDNSARLD
                     LASSDFPERLKTLVPGVVIHCAGPFQGQDYRVAEAAIAAGAHYIDLADGRDFVVRFAG
                     SLDTAARKAGRLAVSGASTLPALSSAVIDVLSKRFRQIEGVQIVIAPGQRAPRGTATI
                     ASVFSYAGRPFQWLSGGVWSNAWGWQELRRVRFAGFGTRWAAACDVPDLELLPERYPG
                     VKAVEFRAALELGIQHFAFWTVAALRRIGVPVPVERWAAQLNRAASWLDAFGSEKGGM
                     LVALAGTRGDSSRARIEWHLTAEASHGPEIPAMAAILLARKLARGELAARGAFPCMGL
                     LTLSEFEAEFAKWRISTVIEETAA"
     gene            1494..1988
                     /locus_tag="AC158Q_00225"
     CDS             1494..1988
                     /locus_tag="AC158Q_00225"
                     /inference="COORDINATES: protein motif:HMM:NF021538.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HIR3338222.1"
                     /translation="MNTALAFWKTAHILSAAILFGTGLGIAFFCWFSYRSAMRSGEIA
                     VLRSALRWTVIADGWITTPAFVFQVVSGIVLMSLMGWPLLSPWSVAAWSLLALVGACW
                     LPVVFIQIGLSREAVRSASVQALPARFHQWFRLWFALGIPAFTTVILIFYLMVAKPLA
                     ISST"
     gene            2018..2455
                     /locus_tag="AC158Q_00230"
     CDS             2018..2455
                     /locus_tag="AC158Q_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003278726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3338223.1"
                     /translation="MPANSADAFEAFFNHAVRLKWDTLLRVNYVEGGGTHPYVGAIST
                     NQGRGWKSGLTMRTRFLSYDPPRQASAEMVEPAGPFALWAASMRFKDHEDGGSDLIYT
                     YSIRLRPRWVGALFDWLAGLLFAWETRRRFKAMENYLRTMKGG"
     gene            2727..2999
                     /locus_tag="AC158Q_00235"
     CDS             2727..2999
                     /locus_tag="AC158Q_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014900293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HIR3338224.1"
                     /translation="MKTRTLPPLRVTPGLRREAESVLADGETLSAFILDSVARNVEAR
                     RAQQAFVARGITSAARAKKSGRYISAEAVMRKLSRRLTAARSKIRG"
     gene            2996..3298
                     /locus_tag="AC158Q_00240"
     CDS             2996..3298
                     /locus_tag="AC158Q_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012338669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIR3338225.1"
                     /translation="MKQYRVRFTPDAEDDLLRLYDFLLEKDLGVAERALEAIRGGIEL
                     LRLSPFSCRKAVADNPLLRELVISFASAGYVALFEIDGPSTVTVLAVRHQREDDYH"
     gene            3395..4159
                     /locus_tag="AC158Q_00245"
     CDS             3395..4159
                     /locus_tag="AC158Q_00245"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR3338226.1"
                     /translation="MPVLESLSFSLSPLELAFCVVALLFAYGLRGSTGFGGALGMAML
                     VVVIPIKVVVPAWTLLSIASSIAILGHDRRRIAWRELLPFLPWCMVGIAIGLYIFTTL
                     DPKTLANSLGVIVFVYAAYALWSTYQPESGWRVPERLLGAVAGTVSGAVGTIFGTMAT
                     PFFVMYLEARKLAKQAFRATMSAMLLALALVRGIGYWAVGEFTQDSLVVFAAAFPVML
                     IGIYAGNRIHLHISELTFKRIVGATLMACSVPLLLK"
BASE COUNT          737 a         1213 c         1411 g          936 t
ORIGIN      
        1 tcggacgatt tctttgctgg ctgttgaatt tgccgaaggc ggcgagcgac gcggagcttg
       61 tattcgagct cgatgcatcc gcgaccaatg agatttggct gcgccatttt ccgggtcgca
      121 ccatgcggtc gacaatgcag attgcggacg gactgttggt cgagctaatc ggctttatca
      181 ggctctggtt tgaattaagc gtggaatccg aaaggctcgt gatgacactc gcgcgcatct
      241 cggtattcgg cctgccgtgg cccggcgcct taatgccgga agtgtggggt ggcgaacgcg
      301 ggaatgcagg gttgctgcat tttgaagcgg gcgctcgtct gccggcgatc ggcctcctga
      361 cttcatataa tggttttctc gttatcaacc ctcaatagta cgtggcggta ctgaacaagc
      421 atgcgggtcg ttgtcctggg tggattcgga aacttcggcg cgcgtatctg ccgggcgctg
      481 gccaatgacc ccggaatcga agtcgttcct gccggccgaa cggatcgagg cgacaacagc
      541 gctcgcctcg atctcgcttc ctctgatttt cccgaaagac tgaagacgct ggtgcctggt
      601 gtggtgattc attgcgcggg gccgttccag ggacaggact accgcgtggc cgaggccgcg
      661 attgcggcgg gtgcgcatta catcgatcta gcggatgggc gggactttgt cgtgcgcttt
      721 gccggaagtc tggatacagc ggcccgcaaa gcggggcggc tggctgtttc gggcgccagc
      781 accctgccgg cgctgtcttc cgccgtgatc gatgtcctgt ccaaacgttt ccgccagatc
      841 gagggggttc agattgtcat tgcccccggc cagcgggcgc ctcgcggcac ggcgacgatt
      901 gcttctgtat tcagctacgc cgggaggcct tttcaatggt taagcggcgg cgtctggtcg
      961 aacgcgtggg gatggcaaga gctgcggcga gtccggttcg ccggtttcgg cacgcggtgg
     1021 gccgctgcgt gcgacgtgcc cgatctcgag ttgctgccgg aacggtatcc tggtgtcaaa
     1081 gcggtggagt ttcgggccgc gctggaattg ggaattcaac acttcgcgtt ttggaccgta
     1141 gctgcgctac gccggatcgg agtgccggtg ccggtggaac gttgggcggc acagttgaac
     1201 agggcggctt cctggctcga cgctttcggc agcgagaagg gcggcatgct cgtggcgctg
     1261 gcgggaaccc ggggcgacag cagcagggcg cgcatcgaat ggcatctcac ggcggaagcg
     1321 agccacggcc cggaaattcc tgccatggcg gcgattctgc tggcgcgcaa gctggcgcgg
     1381 ggagaattag cagcgcgagg cgctttcccg tgcatgggat tgctgacgct gtccgaattc
     1441 gaggcggaat tcgcgaagtg gcgcatttcg acggtcattg aggagacggc ggcgtgaata
     1501 cggcgttagc attctggaaa acagctcata tcctgagcgc cgcgatcctg ttcgggacgg
     1561 gacttggcat cgcgttcttc tgctggttca gctaccgcag tgcgatgcgc tccggagaaa
     1621 ttgccgtcct gcgctccgcg ttgcgatgga cggtcatcgc ggatggctgg atcaccaccc
     1681 ccgcattcgt tttccaggtg gtgagcggga tagtgctcat gagcttgatg ggctggccgc
     1741 tgcttagccc gtggtcagtt gcggcatgga gcttgcttgc tctggtcgga gcctgctggc
     1801 tgccggtggt gttcattcag atcggattaa gccgggaggc tgtacggtcg gcctccgtac
     1861 aggcattgcc ggcgcgtttt catcagtggt tccggctgtg gtttgcactg ggaataccag
     1921 cgttcaccac cgtaatcttg atcttctatc tgatggttgc caaaccgctt gcaatctcca
     1981 gtacatgaat atggcgcacg gaagacttgt tttcataatg ccggcgaaca gcgctgatgc
     2041 gttcgaggca tttttcaatc acgccgtaag actgaagtgg gataccctcc tcagggtgaa
     2101 ctacgtggag ggtggcggca cgcatcccta tgtcggagcc atttccacga accagggccg
     2161 cggctggaaa tcagggctta cgatgcgcac gcgttttctg tcgtacgatc cgccgcgcca
     2221 ggcatcagca gaaatggtcg agccggcggg gccgttcgca ttgtgggcgg catccatgcg
     2281 cttcaaggac cacgaggacg gcgggtcgga tctgatctat acctactcga tccggttgcg
     2341 gccacgatgg gttggcgcgt tgttcgactg gctcgccggt ctgctctttg catgggagac
     2401 gcggcgccgg ttcaaggcca tggagaatta tttgcgaacg atgaaaggcg gataattcaa
     2461 aagagcggtc tcgattgccc gtgccttgtc aattacccat cgccaagacg aagacggcgc
     2521 caggtcttga aatatccctt ctcgaataac gacaggaggc aactattgca gacaacaccc
     2581 agcaaatcgt cgtgaccgat atcaagacac aaggcacaag aaaggcggaa ggatccggtc
     2641 tgagacgaaa gaccggcgtc gggcggggtt ctcccgtctt ctggcgcctg atataatgca
     2701 ttacatttgc aatgcgtgag gtggatatga aaaccaggac gctaccgccc ctgagagtca
     2761 cccccggtct acgccgcgag gcggagtcgg tcctcgcgga cggcgagacg ctgtctgcct
     2821 tcatactcga ttccgtcgcg cgaaatgtcg aggcgcggcg cgcccagcag gcgttcgtcg
     2881 cacgcggtat taccagcgcg gcgcgggcaa agaagtctgg acgctatatt tccgccgaag
     2941 cggtgatgcg aaagctgtcc cgccggctta ccgcggcacg ctcgaagatc cggggatgaa
     3001 gcagtaccgc gtccgtttca ctccggacgc tgaagacgac ctcctcaggc tgtacgattt
     3061 cctgctcgag aaggaccttg gcgtggcgga gcgggcgctg gaagcgatcc gcggcggcat
     3121 cgaattgctt cgcctgtccc cgttctcctg ccgcaaggcg gtggcggaca acccgctgtt
     3181 gcgggagctc gtgatttcct ttgcctcggc gggctacgtg gcgctgttcg agattgatgg
     3241 cccctcgacc gtgaccgtgc tggctgttcg tcatcagcgc gaggacgact atcactgatc
     3301 agctgtggcg taagttcgcg cgctccgcgt gaccgccttg ttttgatact tttcccttgt
     3361 cccttttcac ttttcccttc atcctcgggt tctgatgccc gtcctcgaat cgttgagctt
     3421 ctcgttatcc ccgctcgagc tggctttctg tgtcgtcgcg ctgctgtttg cctacggact
     3481 gcggggcagc accgggttcg gcggcgcgct gggcatggcg atgctggtgg tggtgatccc
     3541 gatcaaggtg gtggtgccgg cgtggacgct gctctccatc gcgtcgtcga tcgcgatact
     3601 ggggcacgat cgcaggcgga tcgcctggcg cgagctcctg cctttcctgc catggtgcat
     3661 ggtgggcatc gccatcggtc tctacatttt cacgacgctc gacccgaaaa cgctcgcgaa
     3721 ctctctgggc gtcatcgtgt tcgtctacgc cgcttacgcg ctgtggtcca cgtaccagcc
     3781 tgagtcgggc tggcgggtgc cggagcgtct gctgggcgcg gtcgcgggca cggtgtccgg
     3841 cgcggtcggc acgatcttcg gaaccatggc gacgccgttt ttcgtgatgt acctggaagc
     3901 gcgcaagctg gcgaagcaag cattccgcgc caccatgtcg gcgatgctgc tcgcgctcgc
     3961 gctggtgcgc ggcatcggct actgggcggt gggcgagttc acgcaggact ctctcgtcgt
     4021 gttcgcggcg gcgtttcccg tcatgctgat cggcatttac gccggcaacc gcatccacct
     4081 ccacatctcc gagctgacct tcaagcgcat cgtcggcgcc accttgatgg cttgcagcgt
     4141 gccgctgctg ctcaagtgag gcgcggcggt aaaggaaaaa ggggaaagga aaaagagaaa
     4201 agggaaaagg aaaaagggtc attggcccga attacgactg ttcccgggtt cttttggcgt
     4261 tgcgcaccag ggtgacgaaa atggaaatca gttgcag
//
LOCUS       DBNGSG010000013         4861 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3908764, whole genome shotgun sequence.
ACCESSION   DBNGSG010000013 DBNGSG010000000
VERSION     DBNGSG010000013.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4861)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3908764"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1278
                     /locus_tag="AC158Q_00250"
     CDS             <1..1278
                     /locus_tag="AC158Q_00250"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HIR3338227.1"
                     /translation="SYFAMNQWLVASLRPPSLKAIIPWEGAADMYRDLAYHGGIFCLG
                     FVGNWFNNHMAHHLFGEPQQHNPDAFHNNWLWQYMRHNLDTGWFDQRRADWGRIEVPM
                     LTAGNWSGMGLHLRGNTEGFVRAASKHKKLRIHAGTHYHPFYSEDGRRDQLRFFDYWL
                     KGEKNGVMDEPPVKLLIRKGGHDNLWRNEREWPLKRTRWTRFYLNPSRVAVKNREAAE
                     GLLATTAPKSKGAVTYSATGMSKAGIASASWTSTVLPGSLPRLGVSFETAPLKEDIEV
                     TGPVVLTLWVASTTEDMDLFATIRNIDRDGRDVWEVGQQQQNVPVAKGWLRASHRALD
                     NTLTRPYRPYHLHDRRQLLKKNEPVRVDVEIWPTCMVFRKGHRIRLDIQPRDGMGSVP
                     YTHYSADYNTGDNTIFTGGGMASHLLLPVIPGK"
     gene            1306..2295
                     /locus_tag="AC158Q_00255"
     CDS             1306..2295
                     /locus_tag="AC158Q_00255"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3338228.1"
                     /translation="MKTRRYLLPVLVAASVLSQSQAFAQARGDDYPSRPVRMIVPFAP
                     GGASDTVGRIIQPAMADLLKQQVVIDNRGGAAGNIGVELAVKANPDGYNFLLGNIGTM
                     AINPNYYTKFPYRPLNDLIPITQIVDVPGSVVVHPSLPAKSVKELISYMKANPGKLNY
                     GAPAPSSANTLETIQFLRATGTDATQVNYKGGAGPATIGLLANEIQLMFVTFSSVVNF
                     SKQGRLRMLGVISPERNPAYPEIPTMREQGLNMVVGSWQGLFVPKGTPQPVVSKLYKV
                     GIEMMKDPRVVKRLGESGITTVTSKSPADFVTFMKAETARFGKVIKDGNIQTQ"
     gene            2390..3658
                     /locus_tag="AC158Q_00260"
     CDS             2390..3658
                     /locus_tag="AC158Q_00260"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR3338229.1"
                     /translation="MANFGIAGLDWQQRVNWERMRNYRLERARERMKAHGLGAMLLMY
                     DENVRYITGTLTPGWNRLKPGLRYAMLCGDDKPVLFEQGDLGFQIERHSPWIPKDHVR
                     YSYAWIKGAAGPASNQQVKKFTNAVKQEMKKANVAGMKLGVDFIDINMLKVFADEKLD
                     WTDGMTAMMEARAVKNVDEQECLRIVGAIGDGAHWEFMKALKPGITENKLTAHIMEYL
                     YSVPGMEDVEDVIVSSGPNTWPNWRNFSDRIVKPGDIVFMDLAALTWNGYKSCYYRTY
                     CAGKEPTKDQKEVYAIALKWLYDSIKAVKVGTTTREIASKWPSAKEAWGYEEEDQAAA
                     NLWGHGLGLAQYDQPVISRIWSLDHPIEIKEGMVFALETQHGRKFEWGVRIEEMLIVH
                     KDKVEIISNFPVEQITVVDPMPGYSTLAGR"
     gene            3698..>4861
                     /locus_tag="AC158Q_00265"
     CDS             3698..>4861
                     /locus_tag="AC158Q_00265"
                     /inference="COORDINATES: protein motif:HMM:NF013491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HIR3338230.1"
                     /translation="MPAVAAMTDYVIPLSASQAGDPASVGPKAANLAALGHAGLPIPD
                     GFCLVADAYRAQVAALGLETTARGVFGSDEGPRARRHALDMKLNLMDKAVPEAISGPL
                     LAARQKLLQRNPGARVVVRSSALVEDRHGSSFAGQFESYLELEDETEFLIAVRACWAA
                     LWSTRALRYMATHDLDPADTAMALLVQPLVRARCAGGALSRTADGDILINATWGLGSA
                     IAQGEVAPDRYVLTRDGELKESITGSMQRQHAASCAHVGRSQIGSSVLAPAPCLDRRQ
                     AAELAGLVRRIDELMGMAVEIEWAMDEAGFKLLQARPLHLQAGAVPDEAWRRHPRING
                     QPAGVGWATGRACVVNCECELIRVAPGDVLVTRMAGPALGQILPRVAGVVAELG"
BASE COUNT          955 a         1567 c         1546 g          793 t
ORIGIN      
        1 tcctatttcg cgatgaacca gtggctggtt gccagcctgc gcccgccttc cctcaaggcg
       61 atcatcccgt gggaaggcgc ggccgacatg tatcgcgacc tcgcctacca cggcggcatc
      121 ttctgcctgg gcttcgtcgg caactggttc aacaaccaca tggcgcacca cctgttcggc
      181 gagccccagc agcacaaccc ggatgccttc cataacaact ggctgtggca gtacatgcgc
      241 cacaacctcg ataccggctg gttcgaccag cgccgggcgg actggggccg catcgaggtg
      301 ccgatgctga cggcgggcaa ctggagcggg atgggcctgc acctgcgcgg caatacggaa
      361 ggattcgtcc gcgccgcgtc gaaacacaag aagctgcgca tccacgccgg cacccattac
      421 caccccttct acagcgagga cggccgccgc gaccagctgc gcttcttcga ctactggctg
      481 aagggcgaga aaaacggcgt gatggacgag ccgccggtga agctcctcat ccgcaagggc
      541 gggcacgata acctatggcg caacgagcgg gaatggccgc tgaagcgcac ccgctggaca
      601 cgcttctacc tgaatccctc ccgggtggcc gtgaagaatc gcgaagcggc cgaggggctg
      661 ctcgccacca cggcgcccaa aagcaagggg gccgtcacct actccgcgac cggcatgtcc
      721 aaggcgggca tcgcctcggc gtcatggact tccaccgtgc tgcccggcag cctgccgcgc
      781 ctcggcgtgt cgttcgagac cgcgccgctc aaggaggaca ttgaggtcac cggcccggtg
      841 gtgttgacgc tgtgggtcgc gagcaccacc gaggacatgg acctgttcgc gacgatccga
      901 aatattgacc gcgacggccg tgacgtctgg gaagtcggac agcaacagca aaacgtgccg
      961 gtggccaagg gctggctgcg cgcctcgcac cgcgctctcg acaataccct cacccgcccc
     1021 taccgcccct accacctgca cgaccgccgg caattgctca agaaaaacga gccggtccgg
     1081 gtggatgtcg aaatctggcc gacctgcatg gtattccgca aggggcaccg cattcgcctc
     1141 gacatccagc cgcgcgatgg gatggggtcg gtcccgtaca cgcactacag cgcggattac
     1201 aatacgggcg acaatacgat cttcaccggg ggcggcatgg catcccacct gctgctgccg
     1261 gtcattcctg gaaaataaac ggccagacct taaagaggaa atcccatgaa aactcgacgc
     1321 tacctgctcc ccgtccttgt cgcggcatcc gtgctgtcgc agtcccaggc ttttgcgcag
     1381 gccaggggtg acgactaccc gtcgcggccg gtgcgcatga tcgttccgtt tgcgccgggc
     1441 ggggcatcgg acaccgtcgg ccgcatcatc cagcccgcga tggcggacct gctcaagcaa
     1501 caggtggtta tcgacaaccg cggcggcgcg gccggtaata tcggcgtgga actcgcggtt
     1561 aaagccaatc ctgacggcta caacttcctg ctcggcaata tcggcaccat ggcgatcaac
     1621 ccgaactact acaccaagtt cccctaccgg ccgttgaatg acctgatacc gatcacccag
     1681 atcgtggacg ttccgggctc ggtcgtcgtg cacccctccc tgcccgccaa gagcgtcaag
     1741 gagctgattt cctatatgaa agccaacccg ggcaagctca actatggggc gcccgcaccg
     1801 agtagcgcga acaccctgga aaccatccag ttccttcgcg ccaccggcac cgatgccaca
     1861 caggtcaact acaagggcgg ggccgggccg gcgaccatcg ggctgctggc caacgagatt
     1921 cagctgatgt tcgttacgtt ttcctcggtg gtgaatttct ccaagcaagg gcgactgcgc
     1981 atgctgggcg tgatttctcc ggaacgcaat cccgcttatc cggaaatccc gacgatgcgc
     2041 gaacaggggt tgaacatggt tgtcggctcc tggcagggtc tcttcgtgcc caagggcacg
     2101 cctcagcccg tggtcagcaa gctctacaag gtcggcatcg aaatgatgaa agacccccgg
     2161 gtcgtcaagc gtctcggcga gagcgggatc accaccgtga ccagcaagtc gccggccgac
     2221 ttcgtcacct tcatgaaggc agagactgcc cgtttcggga aagtcatcaa ggacgggaac
     2281 atccagactc agtaacccct tatcgcgccg tccccctcgc caaggggaag ggatggaacg
     2341 gggtatggtt taattttcaa ttgcagataa acgaagatcg gagaaagtga tggcgaattt
     2401 cggaatagca ggactggact ggcaacagcg cgtcaactgg gaacggatgc gcaactaccg
     2461 gctcgagcgc gcgcgtgagc gcatgaaggc gcacgggctg ggcgccatgc tcctgatgta
     2521 cgacgagaac gtgcgctaca tcacgggcac cctcaccccg ggctggaacc gcctgaagcc
     2581 cggcctgcgc tatgcgatgc tgtgcggcga cgacaagccg gtcctgttcg agcaaggcga
     2641 cctcggtttc cagatcgagc gccattcgcc gtggataccg aaggaccacg tgcgttattc
     2701 ctatgcctgg atcaagggcg cggcggggcc cgcctccaac cagcaggtga agaagttcac
     2761 caacgccgtg aagcaggaga tgaaaaaggc caatgtcgcg ggcatgaagc ttggtgtgga
     2821 tttcatcgac atcaacatgc tgaaggtctt cgcggacgag aagcttgact ggaccgatgg
     2881 catgacggca atgatggagg cgcgcgccgt caagaatgtg gatgagcagg aatgcctgcg
     2941 catcgtcggc gcgatcggcg acggcgcgca ctgggagttc atgaaagcgc tgaagcccgg
     3001 catcaccgag aacaagctca ccgcgcatat catggagtat ctctattcgg tccccggcat
     3061 ggaggacgtc gaggacgtca tcgtctcgtc cggcccgaac acctggccga actggcgcaa
     3121 tttctccgac cgcatcgtca agcccggcga catcgtgttc atggatctcg ccgcgcttac
     3181 ctggaacggc tacaagtcgt gctattaccg cacctactgc gcgggcaaag agccgacgaa
     3241 ggatcagaag gaggtctacg cgatcgcgct caaatggctc tacgactcga tcaaggcggt
     3301 gaaggtcggc accaccaccc gcgagatcgc ctcgaagtgg ccttccgcca aggaggcgtg
     3361 gggctacgag gaagaggacc aggccgcggc caacctatgg ggtcacggcc tcgggctcgc
     3421 ccagtacgac cagccggtca tctcgcgcat ctggtcgctc gaccacccga tcgagatcaa
     3481 ggaaggcatg gtgttcgcgc tcgaaacgca gcacggcagg aaattcgagt ggggcgtgcg
     3541 catcgaggag atgctgatcg tgcacaagga caaggtcgag atcatctcca acttcccggt
     3601 cgaacagatc acggtcgtcg acccgatgcc ggggtactcg accttggccg gccggtaaca
     3661 accctagccg catcgggcat ggagggaatg caactccgtg cccgccgtcg ccgccatgac
     3721 cgattacgtc attccgctta gcgcatcgca agccggagat cctgcgagcg tcggccccaa
     3781 ggccgcgaat ctcgctgcgc tcgggcatgc gggcttgccg ataccggacg gtttttgcct
     3841 ggttgcggat gcctatcgcg cccaggttgc cgcgctggga ctcgaaacca cggcgcgggg
     3901 agtattcggc tcggacgaag gcccgcgggc gcgccgtcat gcgctcgaca tgaaactcaa
     3961 tctcatggac aaagccgtgc ccgaagcgat aagcgggccg ctgctggcgg cacggcagaa
     4021 gctgctgcaa cgcaatcccg gcgcgcgggt cgtagtgcgc tcatccgcct tggtcgagga
     4081 tcgccacggc tcgagctttg ccggccagtt cgaaagctac ctcgagctgg aggatgaaac
     4141 cgagttcctc atcgccgtgc gcgcgtgctg ggccgcgctg tggtcgacgc gtgcgctgcg
     4201 ctacatggcc actcacgatc tcgaccccgc cgacacggcc atggcgctgc tggtgcagcc
     4261 gctagtgcgc gcgcgctgcg ccggcggcgc gctcagccgc acggccgatg gcgacatcct
     4321 tatcaacgcg acgtggggcc tcggctcggc gatcgcacag ggcgaggtgg cgcccgaccg
     4381 ctacgtcctg acccgggacg gagagctcaa ggagtccatc accggctcga tgcagcgcca
     4441 gcacgccgcg agttgcgcgc atgtcggccg ttcgcaaatc ggcagcagcg ttctcgctcc
     4501 cgcgccgtgc ctcgacaggc ggcaggcggc cgagctagcg ggcctggtgc gcaggatcga
     4561 cgaattgatg ggcatggcgg tagagattga atgggcaatg gatgaggcgg gtttcaagct
     4621 gctgcaggca cgaccgctgc acctgcaggc aggcgcggtg ccggacgagg cctggcggcg
     4681 ccacccccgc atcaacggtc agcccgccgg cgtcggctgg gcgaccgggc gcgcatgcgt
     4741 ggtcaactgc gagtgcgagc taatccgcgt ggcgcccggc gatgtgctcg tgacgaggat
     4801 ggccggtccg gcactgggcc agattctgcc gcgcgtcgcc ggcgtggtcg ccgagctcgg
     4861 c
//
LOCUS       DBNGSG010000014         4882 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1871570, whole genome shotgun sequence.
ACCESSION   DBNGSG010000014 DBNGSG010000000
VERSION     DBNGSG010000014.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4882)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4882
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1871570"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..2123)
                     /gene="uvrB"
                     /locus_tag="AC158Q_00270"
     CDS             complement(54..2123)
                     /gene="uvrB"
                     /locus_tag="AC158Q_00270"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HIR3338231.1"
                     /translation="MNRSTPPKAESRDAPELVAFPDSPYRLNPLFPPAGDQPEAIAKI
                     TAGVRDGLAYQTLLGVTGSGKTYTMAHVIGRLGCPALIMAPNKTLAAQLYSEMREFFP
                     ENAVEYFVSYYDYYQPEAYVPSKDLFIEKDSSINEHIEQMRLSATKSLLERRDTIIVC
                     TVSAIYGIGDPVDYHSMILHLREREKLAQRDAIQRLAAMQYERNEIEFRRGVFRVRGE
                     VLDIFPAENSETAVRLTLFDDEVESIHLFDPLTGHVLQRVPRFTVYPSSHYVTPRATV
                     LRAIEAIKEELRGRVDWFQKQNKLVEAQRIEQRTRFDLEMLAEMGFCKGIENYSRHLS
                     GREPGEPPPTLIDYLPHDALMILDESHVTVPQLGGMYRGDRSRKENLVDYGFRLPSAL
                     DNRPLRFDEFEKVTRRTIFVSATPAEYERTHQAQVIEQVVRPTGLVDPAVEVRPATTQ
                     VDDLLSEINDRVKVNERVLVTTLTKRMAEDLTDYLGEHGVKVRYLHSDIDTVERVEII
                     RDLRLGEFDVLVGINLLREGLDLPEVSLVAILDADKEGFLRSERSLIQTIGRAARHIN
                     GAAILYADRVTDSMSRAIGETDRRRRKQVAYNEAHGITPKGVSKRIKDIIEGVYDHDE
                     ARSQLKAAQARERYDSMDEKSLTREVKQIEREMLDAARNLEFERAAQLRDRLKALKER
                     LFIGLAA"
     gene            2293..3498
                     /locus_tag="AC158Q_00275"
     CDS             2293..3498
                     /locus_tag="AC158Q_00275"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HIR3338232.1"
                     /translation="MSQSPLTGIEMAPRDPILGVTETYNADTNPHKVNLGVGVYYDDQ
                     GKVPVLECVRRAEQTLAERPLPRNYLPIDGIPAYDRAVQEVLFGADSPALREQRIVTV
                     QTLGGTGGLKVGADLLRRLNSNAQLWISDPSWENHQALFDYAGFKVQSYPYYDASTHG
                     VRFDAMIGALNKLPAGAIAVLHACCHNPTGVDLSPAQWEQVIEVVNARGLVPFLDIAY
                     QGFAESLDADAAAVRRFAQACPVVFVSSSFSKSLSLYGERVGALSIVTRSADEAIRTL
                     SQLKRVIRTNYSNPPTHGGQAVAMVLTTPELRSLWDKELGQMRDRIKTVRRQLVDKIR
                     GIRSDFDFSFVVRQRGLFSYSGLSRDQVKRLREEYSLYAIDSGRICVAAINSGNIDHV
                     ARGIAGVLV"
     gene            complement(3695..>4882)
                     /locus_tag="AC158Q_00280"
     CDS             complement(3695..>4882)
                     /locus_tag="AC158Q_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338233.1"
                     /translation="PHVAVGGITVGKSADIRVGKVTVTPDLWSLLGDTKVIRSIEIDG
                     LVLTQKAVDKIPAWTTTTAKPGAAAQPPAVRIGSVSLDGAVIKLEKATVGPFDAKLSL
                     AGDGNLESASIISRDGKLKAIIKPHRANYLIDLTAKGWKLPVGPPIHFDELVIKGAAT
                     PKDASLGEIRAKLYGGSVSGKLTAGWGKGIQLKGHAEVNQVEIQPVLQALGKPPTLSG
                     KLTARPVAFSANASGPDQLANALRLGTPFNVQNGVLNGIDIKKAATSLISRDGGKGGE
                     TRFDKLSGHLAMDRGTRRLTQLSVVTGSLSADGNVTVSPKDELSGRMNTRVKAASVTA
                     ATVPLNVSGTVDSPLLFPTGGTIAGAAAGTAVLGPGLGTSVGAKVGQWTEGLFGKKEE
                     KKK"
BASE COUNT          818 a         1568 c         1513 g          983 t
ORIGIN      
        1 acgatctggt ccgctgagcg gcggcaagca gaatcacctg gctctccgca cgctcacgca
       61 gcaagcccga tgaacagccg ttccttcagc gctttcagcc ggtcgcgcag ctgggcggcg
      121 cgttcgaact cgagattgcg cgctgcatcc agcatttctc tttcgatctg cttcacttcc
      181 cgggtgagcg acttctcgtc catcgaatcg taacgttccc gggcctgtgc cgcttttagc
      241 tggctgcgag cctcgtcgtg atcatagacg ccttctatga tgtccttgat gcgcttggag
      301 acacccttcg gcgtgatgcc gtgagcttcg ttataggcca cctgtttacg gcggcggcga
      361 tcggtttcgc cgatggcgcg gctcatggaa tcggtgacgc ggtcggcata gaggatggcg
      421 gcgccgttga tgtggcgcgc cgcccgtccg atggtctgga tcagcgagcg ctccgaacgc
      481 aggaagccct ccttgtcggc gtcgaggatc gccaccagcg acacttcggg caagtccagc
      541 ccttcgcgca acaggttgat gccgaccagc acgtcgaact cgcccaggcg caggtcgcgg
      601 atgatctcca cgcgttccac ggtgtcgatg tcggaatgaa gatagcgcac cttgacgccg
      661 tgctcgccta gatagtcggt caggtcttcg gccatgcgct tggtcagggt cgtcaccagc
      721 acgcgctcgt tgacttttac ccgatcgttg atttcggaca gcagatcgtc gacctgggtc
      781 gtggcggggc gcacctcgac ggccggatcg accagcccgg tggggcgcac cacctgctct
      841 ataacctgcg cctggtgcgt ccgctcgtat tcggccgggg tcgcggaaac gaagatcgtg
      901 cgtcgcgtca ccttctcgaa ctcatcgaac cgcagcggtc gattgtccag cgccgagggc
      961 agccggaagc cgtagtcgac caggttttcc ttgcgtgagc ggtcaccgcg atacatgccg
     1021 ccgagctgcg ggacagtgac gtggctctcg tccaggatca tcagcgcatc gtgcggcagg
     1081 tagtcgatca gtgtcggcgg cggctcgccc ggctcgcgcc cggacagatg ccgcgagtaa
     1141 ttttcgattc ccttgcaaaa ccccatttcg gcgagcatct cgaggtcgaa acgggtgcgc
     1201 tgctctatgc gctgggcttc gaccagcttg ttctgcttct ggaaccagtc tacgcgcccg
     1261 cgcaactcct ccttgatcgc ctcgatcgcc ctcaggacgg ttgcgcgcgg cgtgacatag
     1321 tggctggagg ggtaaacggt gaaccgcggc acgcgctgca gcacgtgtcc cgtcagggga
     1381 tcgaacaggt ggatggactc gacctcgtcg tcgaacaggg tcaggcgcac ggcggtttcc
     1441 gagttctccg ccgggaagat gtccagcact tcgccgcgca cccggaacac gccgcgccgg
     1501 aactcgattt cgttcctctc atactgcatc gcggccaggc gctggatagc gtcgcgctgg
     1561 gcgagctttt cccgttcccg caggtgcagg atcatgctgt ggtagtccac cgggtcgccg
     1621 atgccgtaga tcgccgagac ggtgcagacg atgatggtgt cgcggcgttc gagcagagac
     1681 ttggtggccg aaagccgcat ttgctcgatg tgctcgttga tgctggagtc cttctcgatg
     1741 aagaggtcct tcgagggcac gtaggcttcc ggctggtaat agtcgtaata cgaaacgaaa
     1801 tactcgaccg cgttctccgg gaagaactcg cgcatctcgg agtagagttg tgccgccagc
     1861 gtcttgttcg gcgccatgat cagcgccggg caaccgagcc ggccgatgac gtgagccatg
     1921 gtgtaagtct tgcccgagcc ggtcaccccg agcagggtct gatacgccag cccgtcgcgc
     1981 acgccggcgg tgatcttggc gatcgcctcc ggctggtcac ccgccggggg aaacagcgga
     2041 ttcagccgat acgggctgtc gggaaatgct accagctccg gcgcgtcgcg cgattctgct
     2101 ttggggggcg tggatcggtt catcaactgg atgggcttcg cagccaaacc ttggattttc
     2161 ccatagcagg aggcactgcc gccactttgt cttcggcgcg acctgttcca gttacgtcct
     2221 ggcgcgtctg cgggtaaaat aacgtcccgt tggaagattt ttcatcaatc aaagtccctc
     2281 ctcacccaca aaatgagcca atccccgctc accggcatcg aaatggcgcc acgggacccc
     2341 atcctgggcg tcaccgagac ctataacgcg gataccaacc cccacaaggt gaacctgggc
     2401 gtcggcgtct actatgacga ccagggcaag gtccccgtgc tcgagtgtgt gcggcgcgcc
     2461 gagcagacac tggcggaacg cccgttaccg cggaattacc tgccgatcga cggcataccg
     2521 gcttatgacc gggcggtcca ggaggtgctg ttcggggccg acagcccggc attgcgcgaa
     2581 cagcggatag tgactgtaca gactctcggt ggaaccggcg gactcaaggt cggcgccgac
     2641 ctgttgcgcc ggctgaatag caacgcccag ctctggatca gcgacccgag ctgggaaaac
     2701 caccaggcgc tgttcgatta cgccgggttc aaggtccagt cctaccccta ctacgacgca
     2761 tcgacccacg gagtccggtt cgacgcgatg atcggtgccc tgaacaagct ccctgcaggc
     2821 gcgatcgcgg tgctgcacgc gtgctgccac aacccgaccg gcgtcgacct gtcgccggcg
     2881 caatgggagc aggtcatcga agtcgtcaat gcccgcggcc tcgtgccctt tctcgacatc
     2941 gcctaccaag gtttcgccga aagcctcgat gccgatgccg ccgcggtgcg ccgcttcgcg
     3001 caggcctgcc cggtcgtgtt cgtgtcgagt tcattttcga aatcgctttc tctctacggt
     3061 gaacgggtcg gggcgctctc gatcgtgacc cggagcgcgg acgaagcgat ccggacgttg
     3121 agccagttga agcgcgtgat ccggaccaac tattccaatc ccccgaccca tggaggccag
     3181 gcggtcgcca tggtgctcac cacgccggaa ctgcggtcgc tgtgggacaa ggagttggga
     3241 cagatgcgtg atcgcatcaa gacggtgcgg cgtcagctgg tggacaagat ccgcggcatc
     3301 cgctcggact ttgatttcag tttcgtcgtc aggcagcgcg gtttgttctc ctattccggg
     3361 ctgtcccgcg atcaggtgaa acgtctgcgc gaggaatatt cgctctacgc cattgacagc
     3421 gggcgcatct gcgtcgccgc gatcaattcc ggcaacatcg atcacgtcgc gcgcgggatt
     3481 gccggcgtgc tggtgtgatc accacgcgat gattcgcgtg gtggtgtgag gagtaagggg
     3541 tgaggaaccc cttacgctat ctcctcacgc ctctctcttc ccccagccgc cttggaacgg
     3601 atattcttac tattgatcac ggagcgtcgg ccgcctgcgc acctccgggg tcaaaccccc
     3661 ggtaaacggg ccctctcttc caccctccgg tgcgctactt cttcttttct tccttcttgc
     3721 cgaacaaccc ttccgtccat tgccccactt tcgccccgac cgaggtgccc aatcccggcc
     3781 cgagcaccgc ggtgccggct gcagcgcctg caatcgttcc gccagtgggg aacagcagcg
     3841 gtgagtcgac cgtgccggaa acgttgaggg gcacagttgc tgccgtgacc gaggctgcct
     3901 tgacgcgggt attcatccgg cccgagagct cgtccttggg cgataccgtc acgttgccgt
     3961 cggccgacaa cgaacccgtt acgacgctta actgggtcag gcgccgggtt ccccgatcca
     4021 tggcgagatg gccggatagc ttgtcaaagc gcgtctcccc acccttgccg ccatccctgc
     4081 tgatgagcga cgtggcggcc ttcttgatat cgatgccgtt gagaaccccg ttctgcacgt
     4141 tgaacggcgt ccccaggcgc agcgcgtttg cgagttgatc gggcccggaa gcattggccg
     4201 agaacgcgac cggcctggcg gtcagcttgc cgctcagcgt ggggggcttg ccgagcgctt
     4261 gcagcaccgg ctggatctcg acctggttga cctcggcatg gcccttgagc tgtatcccct
     4321 tcccccagcc cgctgtcagc ttgccggaca cgctgccgcc ataaagcttc gcgcgaatct
     4381 cgccgaggct ggcatccttt ggcgttgccg cgcccttgat cacgagctcg tcgaaatgga
     4441 tcggaggtcc gacaggcaac ttccatccct tggccgtgag gtcaatgagg taattggccc
     4501 ggtgcggctt gatgattgcc ttgagcttgc cgtcgcgact gatgatggaa gcgctctcga
     4561 gattgccgtc acctgcaaga ctcaacttgg cgtcgaacgg tccgacggtg gccttctcca
     4621 gcttaatcac ggcgccgtcg agactgacgc tcccgattct cacggccggc ggctgcgcgg
     4681 ctgcgccggg cttggcggta gtcgtggtcc acgccgggat cttgtctacc gccttctgag
     4741 tgagcaccag cccgtcgatt tcgatgctcc tgatcacctt ggtgtcgccc agcagcgacc
     4801 agagatcggg agtaacggtc actttcccga ccctgatgtc cgcggacttg ccgaccgtga
     4861 tgccgcccac cgcgacgtgc gg
//
LOCUS       DBNGSG010000015         2793 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_343283, whole genome shotgun sequence.
ACCESSION   DBNGSG010000015 DBNGSG010000000
VERSION     DBNGSG010000015.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_343283"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(353..1606)
                     /locus_tag="AC158Q_00285"
     CDS             complement(353..1606)
                     /locus_tag="AC158Q_00285"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3338234.1"
                     /translation="MGHDAGTMPPAPQRNLLPLAVCALGFAQITAWGTSYYCLGVLAK
                     PIAADTGWSHSLVYFGFTVSLLVMGAVSAWAGRAIDRHGARAVMTAGTVIVSAGLYAL
                     SRVTSEGGYLAVWALLGIGMRLCLYDAAFAALVQVVPSRGRLAISYLTLFGAFASTVF
                     WVVGHYLNEAGGWRQTLALFAAINLAVCLPLNWIGLARREPADRPAAATVAAGPAADG
                     PPLEGSRRVVAMALFALVMSLNGFVFGVVTVQLVPLLEAAGLAAAAAVWVASMKGFAQ
                     FGGRVVEIAFARNLHAVTVARIAIGALPVSFFLLFFSGGQLAAIVAFTLLMGASQGVI
                     TIVRGAVPLALFGPVGYGAMLGVLAAPVLVVNAAAPTLFALIVDQWGWPAGQAVLVAV
                     SAASWGAIELMSRWYRRGLAEKRES"
     gene            1681..2541
                     /locus_tag="AC158Q_00290"
     CDS             1681..2541
                     /locus_tag="AC158Q_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3338235.1"
                     /translation="MPVANVRGAAIHYEVYGERGPWVALSPGGRRAIEGVESLSRRMA
                     AAGYRALVHDRRNCGASDVSIEGNESENEIWADDLHELLKQQKALPAWIGGSSSGCRL
                     ALLFALRHSRAARGLLLWRVTGGLAAAKRLAHRYYGQYIPIAQQGGMAAICATGEFAE
                     RIRDRPENRERLMKMNPARFIEVMSGWQRGFLEGADKPVIGATEAQLKVLAVPACIVP
                     GNDLSHLKSAAENLGRLMPDAEVHPLITKHYDLDVAPREEWDAREADLAAIFLDYMKR
                     VEARAAVHVQ"
BASE COUNT          530 a          951 c          915 g          397 t
ORIGIN      
        1 catggcgacc aagatgccct ttgtcggccg cggctgacgc aaagaactct gccacggaac
       61 cacacggaag cacacggaca ttacggaaga tgcaaaccac tcgaccgaac ctgatctacg
      121 acaggtagcg cttgaccaat ccaatcctaa aacgtccgtg aaggcctgga agcggccaag
      181 agtgggcgtt tgcccacttg ttacgtagcg caactgtttc gggttttgaa atttccgtgt
      241 gtttccgtgt gattccgtgg ctaatgactt tcagccgacg ggtaacgaag tgacgcagtg
      301 acgctcccac caccccggcg cttcgcgcca cccctcctcc accaggaggg ggtcacgatt
      361 cacgcttttc tgccagaccg cgccggtacc agcgcgacat cagttcgatc gcgccccacg
      421 atgcggcgga gaccgccacc agcaccgcct ggcccgccgg ccagccccat tggtcgacga
      481 tcaacgcaaa tagcgtgggc gccgcggcgt tgacgaccag cacgggcgcc gcgagcactc
      541 cgagcatggc gccgtagccg accgggccga acagcgcaag cggcaccgcc ccgcgcacga
      601 tcgtgatgac gccctgcgag gcgcccatca gcagcgtgaa ggcgacgatg gcggcgagct
      661 ggcctcccga gaaaaacagc aggaagaagg aaacgggcag cgcgccgatc gcgatgcgcg
      721 ccacggtcac ggcgtggagg ttgcgggcga aggcgatttc caccacgcgc ccgccgaatt
      781 gcgcgaatcc cttcatcgag gcgacccaca cggccgcggc ggcggcgagt cccgcggcct
      841 ccagcagcgg gacgagctgc accgtcacca cgccgaacac gaaaccgttc agcgacatca
      901 ccagcgcgaa cagcgccatc gcgacgacgc gccggcttcc ttcgagcggc gggccgtcgg
      961 cagccggccc cgccgcaacg gtcgccgcgg cgggccggtc cgccggctcc ctgcgcgcaa
     1021 gcccgatcca gttgagcggc aggcataccg cgagattgat cgccgcgaac agcgcgagcg
     1081 tctgcctcca gccgccggct tcgttgaggt agtggccgac cacccaaaat acggtggaag
     1141 cgaatgcgcc gaagagcgtg aggtacgaga tcgcaaggcg cccgcgcgaa ggcacgacct
     1201 gcaccagagc ggcgaacgcc gcgtcgtaga ggcaaaggcg catgccgatc ccgagcagcg
     1261 cccagacggc gaggtagccg ccctcggatg tcacacgcga cagcgcgtag aggcccgccg
     1321 acacgatcac cgttcccgcg gtcatgaccg cgcgcgcgcc atggcggtct atcgcgcgcc
     1381 cggcccacgc cgagaccgcc cccatcacga gcagcgaaac cgtgaacccg aagtagacga
     1441 ggctgtggct ccagccggta tcggcggcaa tcggcttggc cagcacgccg aggcagtagt
     1501 aggacgtgcc ccatgccgtg atttgcgcga agccgagggc gcagaccgcg agcggcagca
     1561 ggtttcgttg cggcgcgggc ggcatggtgc cggcatcatg gcccaactgc ggccgtgaag
     1621 ccagcgacgc cggcggacag tgctatattt ccgcattcga tccgcgtcca gggagcctcg
     1681 atgcccgtcg caaacgtccg cggtgccgca atccattacg aggtttacgg cgagcgcggc
     1741 ccgtgggtgg cgctatcgcc cggcgggcgt cgggcgatcg agggcgtcga gtccctctcg
     1801 cggcgaatgg cggccgccgg ctaccgcgcg ctggtccatg accggcgcaa ttgcggcgcc
     1861 tccgatgtct ccatcgaggg gaacgagtcc gagaatgaga tctgggccga cgatctccat
     1921 gaactgctca agcagcaaaa agccctgccc gcatggatcg gcggcagctc ctcgggatgc
     1981 cggctcgcgc tgctgttcgc cctgcggcac tcccgggccg cgcgcgggct tctgctgtgg
     2041 cgcgtgaccg gcgggctggc cgcggcgaag cggctcgcgc accgctacta cggccagtac
     2101 atcccgatcg cgcagcaagg cggcatggcg gcgatttgcg cgaccgggga attcgcggag
     2161 cgcatccggg accggcccga aaaccgcgag cggctgatga aaatgaaccc ggcgcgcttc
     2221 atcgaggtga tgtcgggctg gcaacgcggc ttcctcgaag gcgccgacaa gcccgtcatc
     2281 ggcgccaccg aagcccagct gaaagtcctc gcggtgccgg cctgcatcgt gccgggcaac
     2341 gatttgagcc atctgaagag tgccgcagag aacctggggc gcctcatgcc ggacgcggag
     2401 gtgcacccgc tcatcacgaa acattacgac ctcgatgtcg cgccgcgcga ggaatgggac
     2461 gcgcgggaag ccgatctcgc cgccatcttt ctcgattaca tgaaacgggt ggaagcccgt
     2521 gcggccgttc atgtgcaatg atccgacgcc gggattccca tcgagcggcg gcagttcatg
     2581 ggttcctgac ttgatctccg aatcactgaa tcgctctatc acttaatcac ttagttagtt
     2641 accgacccca ggggagaccg ctatgactat gagacacggg atatcgatgc tgctgatcac
     2701 ggccgccctc gcctgcgcgg cgccggttca cgcccagctg cagctgggcg ccaacgacac
     2761 ggtggaatcg gtgctgcgcg cccagaagga cag
//
LOCUS       DBNGSG010000016         3602 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2211703, whole genome shotgun sequence.
ACCESSION   DBNGSG010000016 DBNGSG010000000
VERSION     DBNGSG010000016.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2211703"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="AC158Q_00295"
     CDS             complement(<1..499)
                     /locus_tag="AC158Q_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PstC family ABC transporter permease"
                     /protein_id="HIR3338236.1"
                     /translation="MSTISQADGIQVLPDTFVSDRLARRKLRHIKERIIEFVLFLAAL
                     VSVATTVGIVVILLKESLIFFQQVSLWDFLTDTQWTPLFAVAHYGIMPLLSGTLVSST
                     VALLVAIPLGTIIAIYLSEFAPFAVREVAKPFLEMLGGIPTIVYGYFALLFVTPLLQK
                     IYPDLP"
     gene            complement(595..1602)
                     /locus_tag="AC158Q_00300"
     CDS             complement(595..1602)
                     /locus_tag="AC158Q_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR3338237.1"
                     /translation="MNWTGIRTVTAIAAAASILAGFSAPGLAQIIKIDGSSTVFPITE
                     AVAEEFQKSKQGRIKVTVGISGTGGGFKKFCRGETDIQDASRPILRKEMDDCKAAGIT
                     YLEMPVAYDALTVVINPKNSFVKSLTVAELKKMWEPAAQGKVTNWNQINPAFPSSPLK
                     LFGAGADSGTFDYFTEAITGKSKSSRGDFTASEDDNVLVQGVSRDVNSLGFFGFAYYV
                     ENRDKLKAVPIVDKPGGKAVLPSMENVLNGTYQPLARPIFIYVNTAALKRPEIREFVE
                     YYNKHGEKLAREVKYVPLPAKAYSYNLDTMGKMRLGTKFGGENKVGLTIEQLMKMEAR
                     L"
     gene            complement(1775..2011)
                     /locus_tag="AC158Q_00305"
     CDS             complement(1775..2011)
                     /locus_tag="AC158Q_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003262611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HIR3338238.1"
                     /translation="MTDTRFIEELDRKMKEVLARSPAADLEKNLRALLASAFSRLDLV
                     TREEFDVQREVLARTRAKLQELEAKLAELEKRDK"
     gene            2311..3102
                     /locus_tag="AC158Q_00310"
     CDS             2311..3102
                     /locus_tag="AC158Q_00310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HIR3338239.1"
                     /translation="MFKKTAIAGLVAAALGSGLAQAQAPAAPASPHTITGNIGFYSQY
                     IFRGLNQTDGDPALQGGLDYSHTSGFYLGTWASNVSWLRDFGAYTGGASLEWDFYGGM
                     KGTFGKSDFGWDVGLLNYYYPGTVAPGVTKANTLEAYGAVTWKWLSAKYSYSIENTFG
                     VPNSDGTWYLDLSANFPVTDKFSVQAHYGMQEFNGDTAGVSNDSFASYKDWKIGATYA
                     LPKDFTVGAFYTDTDMNATQTAFYTTPAGRFLGKERFTVFISKTF"
     gene            3168..3506
                     /gene="glnK"
                     /locus_tag="AC158Q_00315"
     CDS             3168..3506
                     /gene="glnK"
                     /locus_tag="AC158Q_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HIR3338240.1"
                     /translation="MKLVTAIIKPFKLDEVREALSAIGVTGITVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEAAIKSEILDQVIEAIEKAANTGKIGDGKVFVVDLEQAIRI
                     RTGETGADAL"
BASE COUNT          714 a         1090 c         1072 g          726 t
ORIGIN      
        1 ccggcaggtc gggatagatc ttctgcagca gcggcgtcac gaacagcagc gcgaaataac
       61 cgtaaacgat ggtcggaatg ccgcccagca tctcgagaaa cggtttcgcc acctcgcgca
      121 cggcgaaagg cgcgaactcc gagaggtaga tcgcgataat ggttccgagc ggaatcgcca
      181 cgagcagcgc aactgtcgag ctcactagcg tgcccgataa cagcggcatg atgccgtagt
      241 gcgcgacggc gaagagcggc gtccattgag tatcggtgag aaagtcccac agcgatacct
      301 gctgaaagaa tatcagggac tccttgagca ggatgaccac gatgccaacc gtggtcgcca
      361 ccgataccag ggcggccagg aacagcacga actcgatgat gcgttccttg atgtggcgca
      421 gcttgcggcg cgccagccgg tcgctgacaa aagtatccgg caagacctgt atcccgtcgg
      481 cctgggaaat cgtggacatt attgcagctt gacacgcatt attgcaggtt ttcctgagac
      541 tcgaaaaaac gggaggggtg tgtcacccct cccgtatagc taagcgtctg acgatcaaag
      601 cctggcttcc atcttcatca attgctcgat ggtgaggcca accttgtttt caccgccgaa
      661 tttggtgccg aggcgcattt tgcccatcgt atcgagattg taggaataag ccttggcggg
      721 cagtggaacg tacttcactt ccctggccag tttctcgcca tgcttgttgt agtactcgac
      781 gaactccctg atctccggcc tcttcagcgc ggcggtgttg acatagatga agatgggccg
      841 tgcaagcggc tggtaggtgc cgttcagcac gttctccatc gacggcagca ctgctttgcc
      901 gccaggcttg tccacgatcg gcacggcctt gagcttgtca cggttctcca cgtagtaggc
      961 gaagccgaag aaacccagcg aattgacatc gcgcgacacg ccttgcacca agacgttgtc
     1021 gtcctcactt gccgtgaagt cgccgcggct cgacttggac ttgccggtaa tcgcctcggt
     1081 gaagtagtcg aacgtgccgg agtcggcgcc cgcgccgaag agcttcaggg gcgagctggg
     1141 aaacgccggg ttgatctggt tccagttggt gaccttgccc tgggccgccg gctcccacat
     1201 cttcttgagt tcggctacgg tcaggctttt cacgaagctg tttttcgggt tgatcacgac
     1261 cgtcagcgcg tcgtaggcga ccggcatttc gagataggtg atccccgccg ccttgcagtc
     1321 gtccatctct ttcctgagta tgggccgcga cgcgtcctgg atgtcggtct cgccgcggca
     1381 gaacttcttg aagccgccgc cggtgccgga tatacccacc gtcaccttga tccggccctg
     1441 cttggacttc tggaattcct cggcaacggc ctcggtgatg ggaaaaaccg tgctggaacc
     1501 gtcgatcttg atgatttgag ccagtcccgg tgcggagaag ccggccagaa tcgatgcggc
     1561 ggcggcgatt gccgttactg tgcggatgcc tgtccaattc atatcgtcct ctcgtgtgtt
     1621 tgcccgtgat aagtcccggg ccacgagaat acgcggcagg catgtcgatt ttgttacggc
     1681 cggaacaacg gagcggctaa acgcgggata ggggatacgg gacagggtta ttgttgcctg
     1741 cccgctgcgc gggagcccta tcccctatca ctcatcactt atcacgcttc tcaagctcag
     1801 ccagcttcgc ctccagctcc tggagcttcg cgcgggtgcg cgccagcacc tcgcgctgca
     1861 cgtcgaactc ctcgcgcgtc accaggtcga ggcggctgaa ggcggatgcg aggagcgcgc
     1921 ggaggttctt ctcgaggtcc gccgccgggc tgcgggcgag aacttccttc atcttccggt
     1981 ccagttcttc gatgaatcgg gtatcggtca tggtcatcca cggttgattc gcatcgcagt
     2041 cattttccta caccctcgcc tcccgcgttg ccgggagcgt tgagattaga ggaaccggcc
     2101 gccggcacgt cccgcagcca tggagcacca tctcgcggca caagcatggt gcggctgcat
     2161 ttttctctgc acgattttgg tgcgctcagg cagagacgcc tcctggcaga ttttctatcc
     2221 ttttgtttct gcaacaatat atggctggca tgatccctgc tcattctgcc tccgcaatat
     2281 tgcacattcg ccaactcgaa aggggtcaac atgttcaaga agacagcaat cgcggggctg
     2341 gtcgcggcag cactcggctc gggcctggcc caggcgcagg ctccggcggc gcccgcttcg
     2401 ccgcacacta tcaccggaaa catcggtttc tacagtcagt acatcttccg cgggttgaac
     2461 cagaccgacg gcgatccggc acttcaaggt ggcctggact atagccacac gagcgggttc
     2521 tacctcggca cgtgggcctc gaacgtcagc tggctgcgcg atttcggcgc ctacaccggc
     2581 ggcgccagcc tggaatggga cttctacggc ggcatgaagg gaacgttcgg caagagcgat
     2641 ttcggctggg acgtgggcct gctcaattac tactaccccg gcacggttgc cccgggcgtg
     2701 accaaggcaa acaccctcga ggcctacggc gcggtcacct ggaaatggct ctccgccaag
     2761 tactcgtaca gcatcgagaa cacgttcggc gtgcccaaca gcgacggtac ctggtatctc
     2821 gacttgagcg ccaacttccc ggtcaccgac aagttctcgg tgcaggcgca ttacggcatg
     2881 caggagttca atggcgacac ggcgggagta tcgaacgact ccttcgcgtc ctacaaggac
     2941 tggaagatcg gcgcgaccta tgcgctgccc aaggatttca ccgtcggcgc gttctacacc
     3001 gacaccgaca tgaatgcgac gcagacggcg ttctacacca cgccggccgg ccgctttctg
     3061 ggcaaggaga ggttcaccgt cttcatctcg aagactttct aacgaagcaa cccggggccg
     3121 gccttcatta acttgtctgg ccggccccct ttcaacgggg aaaccgcatg aaactcgtca
     3181 ctgcaatcat caagccgttc aagcttgacg aagtgcggga agcgctgtcc gccatcggcg
     3241 tgaccggcat caccgtcacc gaggtcaagg gcttcgggcg gcagaagggg cacaccgagc
     3301 tctaccgcgg cgccgaatac gtggtggact ttctgcccaa ggtcaagatc gaggccgcga
     3361 tcaagagcga aatcctcgac caggtcatcg aagcgatcga gaaggcggcc aataccggca
     3421 agatcggcga cggcaaggtg ttcgtcgtcg atctggagca agccatccgc atccgcaccg
     3481 gcgaaaccgg tgccgacgcg ctatagaaac agggagaagc caatgaagaa ctttttcagg
     3541 aaactcgtcg cggtgctggt catggtcggc gtcgtgggtc tcaacacggt gccgctcgct
     3601 gc
//
LOCUS       DBNGSG010000017         8076 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4561, whole genome shotgun sequence.
ACCESSION   DBNGSG010000017 DBNGSG010000000
VERSION     DBNGSG010000017.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 8076)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8076)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8076
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4561"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="AC158Q_00320"
     CDS             complement(<1..406)
                     /locus_tag="AC158Q_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902358 - sulfate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HIR3338241.1"
                     /translation="MTPPSPSPASQWSARLFPFLRWWPRVNRATLRADALAGLTGAII
                     VLPQGIAFATLAGLPPQYGLYAAMVPAVVAALFGSSWHLVSGPTNVISIFLFASLSAL
                     AVPNSQEYISLALTLTFLVGAMQLAMGLARMGA"
     gene            405..542
                     /locus_tag="AC158Q_00325"
     CDS             405..542
                     /locus_tag="AC158Q_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338242.1"
                     /translation="MCCGNIPEMHYPAARTTLAMRATMRITAAGFLGIIARSSLGRIK
                     H"
     gene            552..902
                     /locus_tag="AC158Q_00330"
     CDS             552..902
                     /locus_tag="AC158Q_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3338243.1"
                     /translation="MKISRLIPFATAFTLAVAAFPSQAADAGDPAAGKSKNAMCIGCH
                     GIGGYKTVFPEVYHVPKIGGQHAAYIVKALQEYKSGNRSHPSMRAIAASLSDKDMADL
                     AAYYASDTMKSAAK"
     gene            914..1234
                     /locus_tag="AC158Q_00335"
     CDS             914..1234
                     /locus_tag="AC158Q_00335"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3338244.1"
                     /translation="MNKIILAASCLILAASAPALAGDPAAGQRKSQTCVACHGPDGNS
                     QAPDFPKIAGQHYDYLVKVLKDYKSGARKNPLMAPMVANLTARDIEDLAAYYSSQKGL
                     TLKY"
     gene            complement(1308..1943)
                     /gene="nth"
                     /locus_tag="AC158Q_00340"
     CDS             complement(1308..1943)
                     /gene="nth"
                     /locus_tag="AC158Q_00340"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HIR3338245.1"
                     /translation="MTPRQRHELFSRLRAANPAPRTELAYASPFELLVSVILSAQATD
                     KSVNLATAGLFSKANTPSALLGLGRGGLQRHIRSIGLYRTKAKNILATCRLLIEKHGG
                     EVPRTREELEALPGVGRKTANVILNTAFGQPTIAVDTHIFRVSNRTGIAPGRNVAAVE
                     ERLMRTVPEEFRLDAHHWLILHGRYVCKARKPDCSTCLIRDLCEFRHKTPA"
     gene            2088..2591
                     /locus_tag="AC158Q_00345"
     CDS             2088..2591
                     /locus_tag="AC158Q_00345"
                     /inference="COORDINATES: protein motif:HMM:NF019844.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR3338246.1"
                     /translation="MIQATYICALLGLLLVAAPVAAQQVTVERDSPLYAEPRLDAPQV
                     TQLKQGAAGEVTGRQGGWLNLKTPDGTGWLFSFNVRFPAQKAEGGESGAGQALGRVFG
                     PRRSGSVTSSIGIRGIEEEDLRQASFNAGQMKLLEGFAASKQAAAERARATGLAPATL
                     EYLDARK"
     gene            2588..3562
                     /locus_tag="AC158Q_00350"
     CDS             2588..3562
                     /locus_tag="AC158Q_00350"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HIR3338247.1"
                     /translation="MMRSLKCWIAAVTIAAACGHAGAASIDLGRIFGAGKDLATAAGG
                     MDEKEEISVGREVAGRTLGAAPLVADPELQSYVNGVGRWVASQTKRPDLPWHFGVIDS
                     PAVNAFAAPGGYVLLTRGLYEILDSEAQLAGVLGHEIGHIVKRHHITVMQKSALVSAG
                     SQLAQARDRGMLASNLIGTGAEVFARGLDKSAEFEADQIGIVLAARAGYNPYGLVEVL
                     HKLAARGAEDASLSLMFKTHPHPNERLQQLGDALAPHVAALPSGMEPAIRPVGADVPA
                     AAASKPVAVEGARALQQEEAGPAAAQKPAGKGGFGIDPGSVLRGIFGR"
     gene            complement(3715..4125)
                     /locus_tag="AC158Q_00355"
     CDS             complement(3715..4125)
                     /locus_tag="AC158Q_00355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HIR3338248.1"
                     /translation="MAESSTKAPPVAIIDEQACIGCTLCIEACPVDAIVGAAKLMHTV
                     IAAECTGCKWCLAPCPVDCISMVEAREPLTREERRRRAAQYRSRYLARRARLDRERAE
                     HLAAGSGKTALRKKQAMIERAMQRARQRLRNRQD"
     gene            complement(4220..5239)
                     /locus_tag="AC158Q_00360"
     CDS             complement(4220..5239)
                     /locus_tag="AC158Q_00360"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011884670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HIR3338249.1"
                     /translation="MIYSLVRPLLFALEAETAHHFTLDALRVLAGLGLAPSSGAAPPS
                     CARRVIGLDFPNPVGLAAGLDKNGDCIDALARLGFGFIEIGTVTPRPQPGNPRPRLFR
                     LPAARAIINRMGFNSAGVDCLVENVKRARYRGVLGINIGKNADTPIEHAADDYVACLR
                     KVYALASYVTVNISSPNTKHLRELQQDAALDGLLGTLKVEQRRLADAHGKQVPLAVKI
                     APDLDAGQIAAIAGLLRRHRVEAVIATNTTTARDGVVGMANADEAGGLSGAPVTERSS
                     RIVQELSGALQGELPVIGVGGIMSGADARGKIAAGASLVQLYTGLVYRGPELVRECVA
                     ALCAP"
     gene            5410..6477
                     /locus_tag="AC158Q_00365"
     CDS             5410..6477
                     /locus_tag="AC158Q_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR3338250.1"
                     /translation="MVTEKRIEQVIQISAIVLLVVGCLLVLKPFLGAILAAAILCFST
                     WPVYRFIEVRLGGRAWLAALVMTLLILFVLVVPLALIALSFRDDVPQLIENVREVLAH
                     GMPAPPDWVKAIPLVGEQIDAAWRELAESPAALAETLKRFSQPAREVLVQTGLIIAEG
                     ALQFTLIAFVSYFFFRDGAALVGALRNALNRVAGDLTERLLGIVGGTINGVVYGIVGT
                     GLAQGLVALIGFVIAGVPGVIMLASLTFILSIVPAGPPVIWIGATVWLAYHDQPGWAV
                     FMAIWGFVVISGIDNIVKPLLISRGASLPFVLVLLGVFGGILAFGFVGIFLGPTLLAV
                     GFNLTRRWTEARAAEPPPAVG"
     gene            complement(6485..7204)
                     /locus_tag="AC158Q_00370"
     CDS             complement(6485..7204)
                     /locus_tag="AC158Q_00370"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR3338251.1"
                     /translation="MIACHGSRRDFLLRHLGPAFLVAVLLLALETTSIDSAVSNWFFD
                     PVTGAFPLHYNAALEILGHQWPKQLAYVLACCVVATYLLSFVLPALKPQRRLLLFMSL
                     ALTLAPLAVVLLKAASLRHCPWSLREFGGFAQHLSLFDTAPPGLSPGHCFPGGHASAG
                     FCLFAFYFAGLALGNLRLARAGLWGGFVAGMALGMVRVVQGAHFISHNLWSALVCWLV
                     ILALYLAIMGRTTPQPALRAG"
     gene            complement(7201..>8076)
                     /locus_tag="AC158Q_00375"
     CDS             complement(7201..>8076)
                     /locus_tag="AC158Q_00375"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HIR3338252.1"
                     /translation="LNGYPRPTSPELARQHVLSFSNVSACGTSTAVSLPCMFLDVGRA
                     GYDDTLALRREGLLDVLQHAGIKVLWRDNNSGCKGVCDRVPYEDLSHLQVPGLCQNGE
                     CHDEILLHGLQAQIDKLGGDTVIVLHMMGSHGPAYFKRYPPAFEVFTPVCNTVQLDRC
                     SRESIVNAYDNSLLYTDHVLSQTIELLRSNAKRFDTAMLYVSDHGESLGENGLYLHGV
                     PYVLAPREQIEVPMLLWLSDGLRARPGIDAACLEARLREPLAHDPLYHSVLGLTRVQA
                     TGYRPERDLFRRCRT"
BASE COUNT         1420 a         2561 c         2759 g         1336 t
ORIGIN      
        1 gggcccccat gcgcgccagc cccatcgcga gctgcatcgc cccgaccagg aatgtcaggg
       61 tcagcgcgag gctgatgtac tcctggctgt tcggcaccgc gagggcgctc aggctcgcga
      121 agaggaaaat cgagatcacg ttggtcggcc cggagacgag gtgccaggac gatccgaaca
      181 gcgccgcgac gaccgcgggc accatggcgg cgtacaaccc gtattgcggc ggcaggcccg
      241 cgagcgtggc gaacgcgatg ccctggggca gaacaatgat cgcgccggtc agtccggcca
      301 gggcatcggc ccgcagcgtg gcgcggttga cgcggggcca ccagcgcaga aaaggaaaca
      361 gccgtgccga ccactggctt gcgggtgatg gagagggtgg cgtcatgtgc tgcggcaaca
      421 ttcctgaaat gcattatccg gccgcacgca cgacgctggc aatgcgcgcc accatgcgca
      481 tcacagcggc tggatttttg ggtataatcg cgcgcagcag cctgggcaga ataaaacact
      541 aaaagatgac catgaaaatt tcgagactga tcccgttcgc aaccgccttc acccttgccg
      601 tcgcggcttt tccctcccag gccgcggatg ccggtgaccc ggccgcgggc aagagcaaga
      661 atgcgatgtg catcggctgt cacggcatcg gcggctacaa gaccgtgttt cccgaggtct
      721 accatgtgcc caagatcggc ggccagcatg ccgcctatat cgtcaaggcg ctgcaggaat
      781 acaagtcggg caaccgcagc catccctcca tgcgcgcgat cgcggccagc ctctcggaca
      841 aggacatggc cgacctggcg gcctattacg cgtccgacac gatgaagtcg gcggcgaaat
      901 gaggaggaaa cacgtgaaca agatcattct ggccgcttcg tgcctgatcc tggcggcatc
      961 ggcgcctgcc ctggccggtg acccggccgc ggggcagagg aaatcgcaga cgtgcgtggc
     1021 gtgccatggg ccggacggca acagccaggc gccggatttc ccgaagattg cgggccagca
     1081 ctacgattac ctggtgaagg tgctgaaaga ctacaagtcg ggcgcgcgca agaacccgct
     1141 catggcgccg atggtcgcga acctcaccgc gcgcgacatc gaggacctcg cggcgtacta
     1201 ctcgagccag aaagggttga cgctgaagta ctagtggtaa ggggtatggg ataagggagc
     1261 gcggcattgc cgcgcttttt tttcgggtgc caaacccgta tcccctatca cgctggtgtc
     1321 ttatgccgga attcgcacaa gtcgcgaatg aggcaggtcg agcagtcggg ctttctcgcc
     1381 ttgcaaacgt agcgcccgtg caggatcagc cagtggtgcg cgtcgagccg gaattcttcc
     1441 ggcacggtcc tcatcagcct ctcctcgacc gcggccacgt ttctgccggg agcgatgccg
     1501 gtgcggttcg atacccggaa gatatgcgtg tccacggcga tcgtgggctg gccgaacgcg
     1561 gtgttgagga tgacattggc ggtcttgcgc ccgacccccg gcagcgcctc cagttcctcg
     1621 cgtgtccgcg gcacctcgcc gccgtgtttc tcgatgagca gcctgcaggt ggcgaggatg
     1681 ttcttcgcct tggtgcggta gaggccgatg ctcctgatgt gccgctgcag ccccccgcgg
     1741 ccgagcccga gcagggcgga gggcgtgttc gccttgctga acagtcccgc ggtggcgagg
     1801 ttgacgctct tgtcggtggc ctgggccgac aggatgacgg aaaccaggag ctcgaagggc
     1861 gatgcgtagg cgagctcggt ccgcggagcg ggattggccg cgcgcagccg ggagaagagt
     1921 tcgtgccgtt gccggggcgt cataaaggca gttacaagtc acaagtcaca agttgcaagg
     1981 tactacgctc ggcccctggt tggtgaactg gtaacccgaa acctgcaatt tgaaacttgc
     2041 aacttgaaac tgattataat cagcctacct ttccggtccg gtgatcgatg atacaagcca
     2101 cttacatctg tgcgctgctg gggctgttgc tcgtggccgc gccggtggcc gcccagcagg
     2161 tcacggtgga gcgcgactcc ccgctctacg cggagccgcg cctcgacgcg ccccaggtga
     2221 cgcagctgaa acagggtgcg gccggcgagg taaccggcag gcagggcggc tggctgaacc
     2281 tcaagacgcc ggacggaacg gggtggctct tttccttcaa cgtgcgcttt ccggcccaga
     2341 aggcggaagg cggcgagtcg ggtgccggcc aggcgctcgg gcgcgtgttc ggcccgcggc
     2401 gttccggatc ggtcaccagc agcatcggca tccgcggcat cgaggaggaa gacctcaggc
     2461 aggcaagctt caatgccggg cagatgaagc tgctcgaggg ctttgcggcc tcgaagcagg
     2521 cagccgcgga gcgcgcgcgc gcgacggggc tggcgccggc gacgctcgaa tacctcgacg
     2581 cgagaaaatg atgcgctccc tcaagtgctg gattgccgcc gtcacgatcg ctgccgcctg
     2641 cggccatgcg ggcgcggctt cgatcgatct cggccgcatc tttggcgcag gcaaggacct
     2701 ggcgacggcc gccggcggga tggacgagaa ggaagagatc tcggtgggcc gcgaagtggc
     2761 cgggcgcacg ctcggcgcgg ctccgctggt cgcggatccc gagctccagt cctacgtcaa
     2821 cggcgtcggg cgctgggtcg cgtcgcagac caagcgtccc gacctgccct ggcacttcgg
     2881 ggtgatcgac agcccggcgg tgaacgcctt cgccgccccc ggcggctacg tgctgctcac
     2941 gcgcggactc tacgagatcc tcgacagcga ggcgcagctg gcgggggttc tcggacacga
     3001 aatcgggcac atcgtgaagc gtcaccacat cacggtgatg cagaagagcg cgctggtctc
     3061 ggcgggatcc cagctcgcgc aggcgagaga ccgcggcatg ctcgccagca acctgatcgg
     3121 aaccggcgcg gaagtgttcg cgcgcggact ggacaaaagc gccgagttcg aggccgacca
     3181 gatcggcatc gtactcgccg cgcgcgcggg ctacaaccct tacggcctgg tcgaggtgct
     3241 gcacaagctg gcggctcgag gggcggagga cgcttcgctg tccctgatgt tcaagaccca
     3301 cccgcatccc aacgagcgcc tgcagcagct cggtgacgcg ctggcgccgc acgtggcggc
     3361 gctgccctcc ggcatggaac ctgcgatccg gccggtcggc gccgatgtgc ccgcggcggc
     3421 cgcatcgaag ccggtggccg tggaaggcgc gcgggcgctt cagcaggaag aggcgggtcc
     3481 cgcagcggcg cagaagccgg ccggcaaggg cggcttcggc atcgatcccg gcagcgtgct
     3541 gcgcggcatc ttcggccgct gacgcggcaa acaatggaca ggatgaacga catccatcga
     3601 gaagttgcaa acccgccaaa aaccgcaaat agacaggatt cacaagatta acaagattca
     3661 aacagcaaac ccgataattt cgtttttctt tttgatctga tcttgtaaat cctgttaatc
     3721 ctgtctatta cgcagtcgct ggcgggcgcg ttgcatcgcg cgctcgatca tcgcctgctt
     3781 cttgcgaagc gcggtctttc cgctcccggc ggcgaggtgt tccgcgcgct cgcgatcgag
     3841 gcgggcgcgg cgcgccaggt agcgcgacct gtactgggcg gcgcggcgcc tgcgttcttc
     3901 gcgcgtgagc ggctcgcgag cctcgaccat cgagatgcaa tccacgggac agggcgccag
     3961 gcaccacttg cagccggtgc attccgccgc gatcaccgta tgcatgagct tcgcggcccc
     4021 gacgatggcg tccaccgggc aggcttcgat gcacagggtg cagccgatgc aggcttgctc
     4081 atcgatgatg gcgacgggtg gggccttggt ggaactctca gccatgacgg tcacaagtta
     4141 caagttgcaa gttgcaggaa aaccgcggtt tgtgtcttga aacttgtaac ttgaaacttt
     4201 caacccgcgg cgcggcgcgc tatggcgcgc acagcgcggc gacgcattcc cgaactaact
     4261 cggggccgcg gtagacgagc ccggtataga gttggaccaa actcgcgccc gcggcaatct
     4321 tgccgcgggc gtccgcaccg ctcatgatgc cgccgacgcc gatgaccggc aactcgccct
     4381 gcagcgcgcc ggacagttcc tgcacgatcc ggctcgagcg ctccgtgacc ggcgcgccgc
     4441 tcagtccgcc ggcctcgtcc gcattcgcca tgccgacaac gccatcgcgg gccgtggtgg
     4501 tattggttgc gatgacggcc tccacccggt gccgccgcag caggccggct atcgccgcga
     4561 tttggccggc gtccaggtcg ggcgcgattt ttaccgccag cggcacctgt ttcccgtgag
     4621 catcggcgag ccggcgctgc tccactttca gcgtgccgag caggccgtcg agcgccgcgt
     4681 cctgctgcag ctcgcgcagg tgcttcgtgt tgggcgagga aatgttgacc gtcacgtagc
     4741 tcgcgagggc atagaccttg cgcaggcagg cgacgtaatc gtcggcggcg tgctcgatgg
     4801 gcgtatcggc gttcttgccg atattgatgc cgagcacgcc gcggtaacgc gcgcgcttca
     4861 cgttctcgac caggcaatcg acgcccgcgc tgttgaatcc catgcggttg atgatcgcgc
     4921 gcgcggcggg cagccggaac aggcgcgggc gcgggttgcc cggctgcggg cgcggcgtca
     4981 ccgtgccgat ctcgatgaag ccgaatccca ggcgggcgag cgcatcgatg cagtctccgt
     5041 tcttgtcgag gccggccgcg agtccgaccg ggttggggaa gtcgaggccg atgacccggc
     5101 gcgcgcagga cggcggcgcg gcgccggagg agggcgcgag ccccagtccg gcaagcaccc
     5161 gcagcgcgtc gagcgtgaaa tgatgcgcgg tctcggcttc gagcgcgaac agcagcgggc
     5221 gcaccagcga gtaaatcatc gcgctattgt agcgcgatga tttaccagca tgatgagtgt
     5281 cattaaggcg gccgacgcgg tgaattttgc attcgtgaag tcacgtcaga ggccgccttg
     5341 gcggaggccg cctttatttg accgcccgtg cgccgcgtgt tagaacccgc gggcaaggaa
     5401 gcaacgaaca tggtcacgga aaagcgcatc gagcaggtca tccagatctc cgccatcgtc
     5461 ctgctggtgg tgggctgcct gctggtgctg aagccgtttc tcggcgcgat cctcgctgcc
     5521 gcaatcctgt gcttctccac gtggccggtc taccgcttca tcgaagtcag gctgggcggc
     5581 cgcgcctggc tcgccgcgct ggtgatgacg ctgctgatcc tgttcgtgct ggtggtgccg
     5641 ctggcgctga ttgcgctctc cttccgcgac gacgtgccgc aactgatcga gaacgtgcgc
     5701 gaggtgctgg cccacggcat gcccgcgccg ccggactggg tcaaagccat cccgctcgtc
     5761 ggcgagcaaa ttgacgccgc ctggcgcgag ctggcggaaa gtcccgctgc gctggcggag
     5821 acgctcaagc ggttctccca gccagcgcgc gaggtcctgg tgcagaccgg cctcatcatc
     5881 gcggaaggcg ccctgcaatt taccctgatc gcgttcgtca gctacttctt tttccgcgac
     5941 ggcgcggcgc tggtcggagc gctgcgcaac gcgctgaatc gcgttgccgg cgacctgacc
     6001 gagcggctgc tgggtatcgt cggcgggacg atcaacggcg tggtctacgg catcgtcggc
     6061 accgggctcg cgcaggggct cgtggcgctg atcgggtttg tcatcgccgg cgtgccgggg
     6121 gtgatcatgc tggcctcgct caccttcatc ctatccatcg tgcccgccgg gccgcccgtg
     6181 atctggatcg gcgcgacggt gtggctcgcc taccacgacc agccgggctg ggcggtgttc
     6241 atggcgatct ggggattcgt cgtgatcagc ggcatcgaca acatcgtcaa gccgctgctc
     6301 atcagccgcg gcgcgagcct gccattcgtg ctggtgctgc tcggcgtgtt cggcggcata
     6361 cttgccttcg gcttcgtcgg catcttcctc gggcccacgc tgctcgccgt gggcttcaac
     6421 ctcacgcggc gctggaccga ggcgcgtgcg gccgagccgc cgccggcagt gggttgaact
     6481 gagcttaccc cgcgcgcagg gcgggctgcg gcgttgtccg gcccatgatc gccagataga
     6541 gcgccagtat caccaaccag cacaccaggg ccgaccacag gttgtgggag atgaagtgcg
     6601 cgccctgtac gacgcgcacc atgccgagcg ccattcccgc cacgaagccg ccccacagtc
     6661 cggcgcgtgc gagacgcagg ttgccgagtg cgaggccggc aaagtagaac gcgaacaggc
     6721 agaagccggc agaggcgtga ccgcccggaa agcagtgacc gggcgatagg ccgggaggcg
     6781 ccgtgtcgaa cagggagagg tgctgcgcaa aaccgccgaa ttcccgcagg ctccacgggc
     6841 agtgacgcaa gctcgccgcc ttgagcagga ccactgccag cggagcgagt gtcagggcaa
     6901 gcgacatgaa cagcagcagg cggcgctgcg gtttgagtgc cggcagcacg aaactcagca
     6961 ggtacgtcgc gaccacgcaa caggccagga cgtaagccag ctgcttcggc cattggtggc
     7021 cgaggatttc aagcgcggcg ttgtagtgga gaggaaaggc gccggtaacg gggtcgaaaa
     7081 accagttgct taccgctgaa tcaatggaag tcgtttccag ggcgagaagg agaacagcaa
     7141 ccaggaacgc cgggccgaga tgacgcagca gaaaatcccg cctgctgcca tggcaagcaa
     7201 tcaagtccgg caccggcgga acaggtctcg ctccggacga tagcccgttg cctgcaccct
     7261 cgtcaggccc aggaccgaat ggtagagagg gtcgtgcgcg agcggctcgc gcagccgcgc
     7321 ttcaagacag gccgcatcga tgcccgggcg tgcgcgcaag ccgtcagaca gccaaagcag
     7381 catcgggacc tcgatctgct cgcgcggcgc cagcacgtag gggacgccgt gcaagtacag
     7441 tccattctcg cccagggatt cgccgtggtc cgacacgtac agcatcgcgg tatcaaagcg
     7501 ctttgcattg ctccgcagca actcgatcgt ctggctcaat acgtggtcgg tatagagcag
     7561 ggaattgtcg taggcgttca cgatgctctc acgcgaacag cggtcgagct ggaccgtgtt
     7621 gcacaccggt gtgaagacct cgaacgccgg gggatagcgc ttgaagtagg ccgggccgtg
     7681 gctgcccatc atgtgcagca cgatgacggt gtcgccgccc agcttgtcaa tttgagcctg
     7741 cagcccgtgc agcaggatct cgtcgtggca ctcgccgttt tggcacaggc cgggcacctg
     7801 caggtgcgag aggtcctcgt agggcacgcg gtcgcacacg cccttgcacc cggaattgtt
     7861 gtctcgccag agcaccttga tgccggcgtg ctgcagcacg tcgagcagcc cttcgcggcg
     7921 cagtgcgagc gtgtcatcgt agcctgcgcg gccgacatcc agaaacatgc aaggcagcga
     7981 aacggccgtg gaggtgccgc aggcgctgac gttggagaag ctcaacacgt gctggcgcgc
     8041 gagctcgggg ctggtcgggc gcgggtagcc gttgag
//
LOCUS       DBNGSG010000018         6148 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1193096, whole genome shotgun sequence.
ACCESSION   DBNGSG010000018 DBNGSG010000000
VERSION     DBNGSG010000018.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6148)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6148)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6148
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1193096"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /gene="mscL"
                     /locus_tag="AC158Q_00380"
     CDS             complement(<1..275)
                     /gene="mscL"
                     /locus_tag="AC158Q_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HIR3338253.1"
                     /translation="MLKEFKEFAVRGNVVDMAVGIIIGAAFGKIVDSLVKDLIMPPIG
                     LLLGKVDFTNLFFVLKQGAQAGPYLSVEAAQKAGAVTFNYGVFINTL"
     gene            403..1338
                     /locus_tag="AC158Q_00385"
     CDS             403..1338
                     /locus_tag="AC158Q_00385"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008250516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR3338254.1"
                     /translation="MRTLICGSIAYDTIMVFRDRFRNHILPEQLHILNVAFLVPDLRR
                     EFGGCAGNIAYNLSLLGGEPLIMATVGDDYMPYDERLKKLKLPQQHVRRIPGTFTAQA
                     FITTDLDDNQITAFHPGAMNHSHENHVGEAKDVKLGIVAPDGREGMLQHTREFHAAGV
                     PFIFDPGQGLPMFSGDELNGFVDLASYVTVNDYEGQMLQERSGRKLEELAKRVKALVV
                     TLGAKGSLVIANGRSLEIPAVKPAQVVDPTGCGDAFRAGLLYGIAAGLELSVAARLAS
                     LLGALKIASRGGQNHRFTRDEIAERYREAFGSRPW"
     gene            1439..1909
                     /locus_tag="AC158Q_00390"
     CDS             1439..1909
                     /locus_tag="AC158Q_00390"
                     /inference="COORDINATES: protein motif:HMM:NF024879.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein"
                     /protein_id="HIR3338255.1"
                     /translation="MKRFLLVLASAFMLLAGCASAPWDDDGRGHRARPQPEHREYAGV
                     VEGVREVQIDSERTGVAPMVGAVFGGAAGSSVGSGRGAVVGGVVGTVAGAVIGEAAAQ
                     HGSQPGLEVTVRLDEGRVIAVTQPAGETFQPGDPVRVISDGRVARVTKAVTGDR"
     gene            complement(1913..2740)
                     /locus_tag="AC158Q_00395"
     CDS             complement(1913..2740)
                     /locus_tag="AC158Q_00395"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HIR3338256.1"
                     /translation="MATYAIGDVQGCYDQLMRLLEQAGYDERRDMLWFVGDLVNRGPQ
                     SAQVVRFVKGLGERQVTVLGNHDLALLVIAEGIKKPHRGDTFDELLAAPDRDELVDWL
                     RHQKMMHAGGGHAMVHAGLLPQWSLSQAAALAREVEAALQGPDHREFLKHMYGNEPLC
                     WRDDLAGYDRLRVIVNAMSRMRLVTPDGAMEFSHKTGLAGLPSGYLPWYDAPGRATRG
                     ERIYFGHWAALGLMQREDVVCLDTGCVWGRALSALRLEDRRLFQLDCAELAGTASED"
     gene            complement(2844..3392)
                     /locus_tag="AC158Q_00400"
     CDS             complement(2844..3392)
                     /locus_tag="AC158Q_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3338257.1"
                     /translation="MAEQHDEHSSLIKTPKQLIIVVVLAFAVPIVLIVMLTQLITGGT
                     DLGKDNPALSEEAIARRLKPVGEVAVDASQPKPSPAAPVAVAAAPVPGAAIPAAAKGD
                     TGKGKSVYDSACTVCHAAGVAGAPKAGDKAAWAPRLKTGMAALYTSSIKGKSAMPPKG
                     GNLSLSDADVKAAVDYMVGLVK"
     gene            3516..3591
                     /locus_tag="AC158Q_00405"
     tRNA            3516..3591
                     /locus_tag="AC158Q_00405"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3549..3551,aa:Arg,seq:ccg)
     gene            3635..5257
                     /locus_tag="AC158Q_00410"
     CDS             3635..5257
                     /locus_tag="AC158Q_00410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR3338258.1"
                     /translation="MPNPKPVDALIVGGGHNGLTCACYLAGAGYTVKVLERRPVVGGA
                     AVTEEFLPGFRNSAAAYTVSLLHPRVISDLRLAEHGLKIIERPMANFLPLSDSSYLKI
                     GNSLGQTQTEIGRYSARDAERLPDYYAMLERAGSVFREFLLETPPNLGGGARDAFAAL
                     RTAWRVRKSGMAARRDLLDLMAKSAGDLLDGWFESAPIKAAFGFDAVVGNYASPYAPG
                     SAYVLLHHVIGEVNGRRGAWGHAVGGMGAITQAMAKEAARRGVEINVDCEVSKVCVDD
                     RGAQGVQLTDGSMVDARCVVAGIDPKRLFLKLVDERSLDADFVQRVRGYKCGSATFRM
                     NVALSELPDFNCLPGRHLQAHHQSGIIIGPSLAYMEQAYFDARTLGCSRAPIVEMLIP
                     STVDPTLAPPGKHVASLFCQHFAYDLPNGLDWDSIREPAADLVIDTVTRYAPNFKASV
                     LGRRIRSPLDLEREFGLTGGDIFHGALTLDQIWSARPVLGHADYRTPIAGLYLCGAGT
                     HPGGGVSGMPGHNAAREILRDLRKGGNRRKPK"
     gene            5358..5972
                     /locus_tag="AC158Q_00415"
     CDS             5358..5972
                     /locus_tag="AC158Q_00415"
                     /inference="COORDINATES: protein motif:HMM:NF024863.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HIR3338259.1"
                     /translation="MLAVNRFAIALVFAAALVGCGKKVPPLAPVGPNDVIVAFGDSLT
                     YGTGASESESYPAVLAQIINRQVIRSGVPGETTRGGLARLESVIEEHRPVLMIVSLGG
                     NDMLGRVGDAEIKANLRSIIQTIRGRGISVVLFGVPKPALITSAAPFYEELAQEFDIP
                     YEGKIVTDVLYRRDQKSDPIHPNAIGYRRMAEAFAELLKKAGAI"
BASE COUNT         1061 a         2005 c         2028 g         1054 t
ORIGIN      
        1 atcagggtgt tgatgaatac gccgtaattg aaggtgaccg cccccgcctt ctgcgccgct
       61 tcgacggaga ggtaggggcc ggcctgggcg ccttgcttga ggacgaagaa caagttggtg
      121 aaatccactt tgccgagcag caggccgatc ggcggcatga tgagatcttt caccagcgag
      181 tccacgatct tgccgaaggc ggcgccgatg atgatgccga ccgccatgtc cacgacgttg
      241 ccgcgcaccg cgaattcctt gaattccttc agcatgtgat tttcctccag gtacgttggt
      301 cttggcccgg acggaccgtt gcgtcccgca ttatgctatg ccgaaccggc attgtcgcaa
      361 gcggctataa tttcgatgtg attttttcat gatgctcttt tcatgcgcac attgatctgc
      421 ggctcgatcg cctacgacac catcatggtg ttccgcgacc gcttcaggaa ccacatcctg
      481 cccgagcagc tgcacatcct gaacgtcgca ttcctggtgc ctgacctgcg gcgcgagttc
      541 ggaggctgcg ccggcaacat cgcctataac ctctccctgc tgggcggcga gcccctcatc
      601 atggccaccg tcggcgacga ttacatgccg tacgacgagc gcctgaaaaa gctgaagctg
      661 ccgcagcagc acgtgcgccg cattcccggc accttcaccg cgcaggcctt catcaccacc
      721 gacctcgacg acaaccagat cacggcgttt catcccggcg cgatgaacca ctcgcacgag
      781 aaccacgtgg gggaggcgaa ggacgtcaag ctcggcatcg tcgcgcccga cgggcgggaa
      841 ggcatgctgc agcacacgcg cgaatttcac gccgccgggg tgccctttat cttcgacccc
      901 ggccagggcc tgccgatgtt cagcggtgat gagctgaacg gcttcgtcga cctcgcgagc
      961 tatgtgacgg tcaatgacta cgaaggtcag atgctgcagg aacgcagcgg caggaagctg
     1021 gaggaactgg cgaagcgcgt gaaggctctg gtggtaaccc tgggggcgaa aggctcgctg
     1081 gtgatcgcga atggccgtag cctcgagatt cccgccgtga aaccggcgca ggtggtggac
     1141 cccacgggat gcggcgacgc ttttcgcgcc ggcctgctat acggcattgc cgcgggactc
     1201 gaattgtcgg tcgccgcgcg gctcgcatcg ttgctgggcg cgctcaagat cgccagccgc
     1261 ggcggccaga accaccgctt cacccgcgac gagatcgccg agcgctaccg ggaagccttc
     1321 ggctcacggc cctggtgatc aaggcgtgag tcgtgattcg atcggcccag ggtgggggaa
     1381 tgcatgcttg tttcgtaatc gattcaccaa tcaccaatca cgctattgga ttgagggcat
     1441 gaaacggttt ctcctcgtgc tggcgtcggc gttcatgctt ctggccggct gcgccagcgc
     1501 gccgtgggac gacgacgggc gcggccaccg cgcgcgaccg cagccggagc acagggagta
     1561 tgcgggcgtg gtggagggcg tgcgcgaggt ccagatcgac agcgagcgca ccggtgtcgc
     1621 ccccatggtg ggcgcagtgt tcggcggcgc ggccggctcc agcgttggaa gcggccgcgg
     1681 cgcggtcgtg ggcggagtgg tgggtaccgt cgcgggcgcc gtgatcggcg aggcggcggc
     1741 gcagcacggg tcgcagccgg gtctggaggt taccgtccgt ctcgatgaag ggcgcgtcat
     1801 tgccgtcacg caacccgccg gcgaaacctt ccagcccggc gacccggtgc gcgtgatctc
     1861 cgacggccgt gtcgcgcgcg tcaccaaggc ggtgacgggt gacaggtaac ggctagtctt
     1921 cggacgctgt tcccgcgagc tccgcgcaat ccagctgaaa caaccggcga tcttcgagcc
     1981 gcaacgcgct caacgcgcgc ccccacacgc agccggtgtc caggcacacc acgtcctccc
     2041 gttgcatgag gccgagcgcg gcccagtggc cgaagtaaat gcgttcgccg cgggtggccc
     2101 ggcccggcgc gtcgtaccac ggcaggtagc cggaggggag tccggcgagc ccggtcttgt
     2161 ggctgaactc catcgcgccg tcgggggtga ccagccgcat gcgcgacatc gcgttgacga
     2221 tgacccgcag acggtcgtag ccggcaagat cgtcgcgcca gcacaggggc tcgttgccgt
     2281 acatgtgctt caggaactcg cggtggtccg gtccctgcag cgcggcttcc acctcgcgcg
     2341 cgagcgcagc cgcctgcgac aggctccact ggggaagcag gcccgcgtgc accattgcat
     2401 ggccgccgcc ggcatgcatc atcttctgat gacgcagcca gtcgacgagc tcatcccggt
     2461 cgggggcggc aagcagctcg tcgaaggtgt cgccccggtg cggcttcttg atgccctccg
     2521 caataaccag aagcgcaaga tcatggttgc cgagcaccgt gacctgccgc tcgcccaggc
     2581 ctttcacgaa acgcaccacc tgcgcggact gcgggccgcg attgacgagg tcgccgacga
     2641 accacagcat gtcgcgccgc tcgtcgtaac ccgcctgctc gagcaggcgc atgagctggt
     2701 cgtaacagcc ctggacgtcg ccgattgcgt aggttgccat ctggaaacgc gagagtcgta
     2761 attcgtgagt cgtgattcgg gcccaaacaa aaaaggcgca gcgtgcgctg cgccctcact
     2821 catcacccat cacccatcac gcctcattta acgaggccca ccatgtaatc caccgccgct
     2881 ttcacgtcgg cgtcggagag cgacaggttg ccgcccttgg gcggcatggc actcttgccc
     2941 ttgattgagc tcgtatagag cgccgccatg ccggttttca gacgtggcgc ccacgccgcc
     3001 ttgtcgcctg ctttcggcgc gcccgccacg cccgcggcgt ggcatacggt gcaggccgag
     3061 tcgtaaaccg atttgccctt gcccgtatcc cccttggccg cggctggaat tgcagcgccc
     3121 ggcaccggtg ccgctgcgac tgcaaccggc gcggcaggcg acggcttcgg ctggctcgcg
     3181 tcgaccgcca cttcgccgac cggtttcagg cgcctggcga tggcttcctc ggacagcgcc
     3241 gggttgtcct tgccgagatc ggtgccgccg gtgatgagct gcgtcagcat cacgatcaac
     3301 acgatcggca ccgcgaacgc cagcacgacc acgatgatga gctgcttggg tgtcttgatc
     3361 agcgaggaat gttcgtcgtg ttgctcggcc atggcgacgc cttcagcgtg tttcgggaag
     3421 gcgctcatta tagcgtaacg cgccgcaacc ggagcgtaat accttgattt cggggcgata
     3481 gtgcgaatgg cgctatgtta gaattcgttc cgcacgcgcc tgtagctcag aggatagagt
     3541 attggcctcc gaagccaagg gtcgtgggtt cgaatcccgc caggcgcgcc accatcaatc
     3601 aatgagctgg gcgaaatcag cccgctcctg gcccatgccg aatcccaaac cggtcgacgc
     3661 gctcatcgtc gggggcggcc acaacgggct gacctgcgcc tgttacctcg ccggcgcggg
     3721 gtacacggtg aaagtgctcg agcgccgccc ggtggtcggt ggcgcggcag tcacggagga
     3781 gttcctgccg ggcttccgca actccgccgc cgcttatacg gtcagcctgc tgcaccccag
     3841 ggtgatcagc gatctgcggc tcgccgagca cggcctgaag atcatcgagc ggccgatggc
     3901 gaactttctg ccgctctccg acagttcgta cctcaagatc ggcaactcgc tcggccagac
     3961 ccagaccgag atcgggcgat actcggcgcg cgatgccgag cggctgcccg attactacgc
     4021 catgctggag cgcgccggca gcgtgttccg ggagtttctt ctcgagacgc cgccgaacct
     4081 cggcggtggc gcgcgcgatg cgtttgcagc cttgcgcacg gcctggcgcg tgcgcaagtc
     4141 gggaatggcc gcgcgccggg atttgctcga cctgatggcc aagagcgccg gcgacctgct
     4201 cgacggctgg ttcgagtccg ctccgatcaa ggcggccttc ggcttcgacg cggtggtcgg
     4261 caactacgcg agcccgtacg cgccgggctc ggcctacgtg ctgctgcacc acgtgatcgg
     4321 ggaggtgaat ggccgccgcg gcgcatgggg ccatgcggtg ggtggcatgg gcgcgatcac
     4381 gcaggcgatg gcgaaggaag cggcgcggcg cggcgtcgag atcaacgtcg attgcgaagt
     4441 gagcaaggta tgcgtggacg atcgcggggc gcagggcgtg cagctcaccg acggcagcat
     4501 ggtggacgcg cgctgcgtgg tggccggcat cgatccgaag cggctgttcc tgaagcttgt
     4561 cgacgaacgc tcgctcgatg cggacttcgt gcagcgcgtc cgcggctaca agtgcgggtc
     4621 ggcgaccttc cgcatgaacg tcgcgctctc ggagctgccg gatttcaatt gcctgcccgg
     4681 ccggcacctg caggcgcatc accagtcggg catcatcatc ggcccctcgc tcgcctacat
     4741 ggagcaggcc tatttcgatg cgcgcacgct cggctgttcg cgcgcgccca tcgtggaaat
     4801 gctgatcccg tccacggtgg acccgacact cgcgcccccc ggcaagcacg tcgccagcct
     4861 gttctgccag cattttgcct atgatctgcc caacgggctc gactgggact cgatacggga
     4921 acccgcggcc gacctggtga tcgacaccgt cacgcgctac gcgcccaact tcaaggcttc
     4981 cgtgctcggg cgcaggatac ggtcgccgct cgatctcgag cgcgaattcg ggttgaccgg
     5041 cggcgacatt tttcacggcg ccctgacgct cgaccagatc tggagcgcgc gcccggtgct
     5101 gggccacgcc gattaccgca caccgattgc cggtctctac ctgtgcggcg cgggcacgca
     5161 ccccggcggc ggcgtgtcgg gaatgcccgg acacaacgcg gcgcgggaaa ttctgcgcga
     5221 cctgcgaaag ggcggcaacc gcaggaagcc caagtgaggc gaggcctgac gcgctgctag
     5281 aattgcgccc atgcccgcgt ttcctgatcg atcccccgca tgacgatggt gcggcgaaca
     5341 cggctcgcag tgtgcaaatg ctggccgtga accgttttgc catcgcgctg gtcttcgctg
     5401 ccgcgctcgt gggctgcggg aagaaagtgc cgcctctcgc gcccgtaggc ccgaacgacg
     5461 tcatcgtcgc tttcggcgac agcctgacct acggcaccgg cgccagcgag tcggagagct
     5521 atcccgcggt gctggcgcag atcatcaacc gccaggtcat ccgctcgggt gtcccgggcg
     5581 agacgacccg cggagggctg gcgcgcctgg agagcgtgat cgaggagcac cgcccggtcc
     5641 tgatgatcgt gagcctcggc ggcaatgaca tgctgggcag ggtgggcgac gccgagatca
     5701 aggccaacct gcggagcatc atccagacga ttcgcgggcg gggcatctcg gtggtgctgt
     5761 tcggcgtgcc caagcccgca ttgattacca gcgccgcccc gttctacgag gaactggcgc
     5821 aggagttcga cataccctac gagggcaaga tcgtcaccga cgtgctctac cggcgcgacc
     5881 agaaatccga cccgatccat cccaatgcca taggttaccg ccggatggcg gaggcgttcg
     5941 cggagctgtt gaagaaagcg ggcgcgattt gaaaaagcac agtaaccgcc gatgaacgcc
     6001 gatgaacgcc gtaagatgga aaaaagccag ctgagacttc caaggttgct tcgaaaagca
     6061 gtcaccagaa taggaaggca agcgaacggc ggcgggccga atcccgtgga tggcaccctg
     6121 caagtaaatg tgatcggcgt tcatcggc
//
LOCUS       DBNGSG010000019         2516 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3062010, whole genome shotgun sequence.
ACCESSION   DBNGSG010000019 DBNGSG010000000
VERSION     DBNGSG010000019.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3062010"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="AC158Q_00420"
     CDS             <1..333
                     /locus_tag="AC158Q_00420"
                     /inference="COORDINATES: protein motif:HMM:NF016836.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIR3338260.1"
                     /translation="IDSARRRLRYAWILVSMLAALGAFAGASAQDKGARFDDAVITQR
                     VSSALDADPVLRQMHIAVETRDGVVRLTGFVDSTAQIDRAAALARRIEGVSAVRNAIR
                     VINRPSRA"
     gene            366..731
                     /locus_tag="AC158Q_00425"
     CDS             366..731
                     /locus_tag="AC158Q_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338261.1"
                     /translation="MNLPKLPIETKPFLWGAVAGAITLAIVGFTWGGWVTSTTVERLA
                     GARADAATVAALTPICVTQFQASSRAPASLAALKGIQSWEQADYVSKGGWATMPGDNA
                     APSREVAAACAAALIKHAS"
     gene            complement(765..1562)
                     /locus_tag="AC158Q_00430"
     CDS             complement(765..1562)
                     /locus_tag="AC158Q_00430"
                     /EC_number="4.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase family protein"
                     /protein_id="HIR3338262.1"
                     /translation="MATVPNHTLRQLRAGKLAIGLGVNQARTVGIATMAKTAGFDWLF
                     IDGEHSSMDGDDAAQLSTAALGAGVTPIVRVAGFEHWHASRMLDNGAQGIVFPHVDSS
                     QDAQRVANACRYPPIGKRSMGGALAQLGFASMPVGEAARLVNEETLVVVMIESPQGVA
                     NCEEIAAVKGVDALLIGTNDLCFEMGIPGQFNDPRVAEAYRKVIAACRKHGKFPGMGG
                     MYTPELLERHIAMGVQLILSGSDFSLLMQAATARAALVRGFEKKIGV"
     gene            1665..>2516
                     /locus_tag="AC158Q_00435"
     CDS             1665..>2516
                     /locus_tag="AC158Q_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HIR3338263.1"
                     /translation="MKAKVVSVAVLAAAVIGTSAGISHFPVRAASPAAAVQTLPDFAA
                     IAEANKAAVVNITSSAAPKPGADAPEGLENLPEELRRFLPQIPPQPRQGMGSGFIVES
                     DGTILTNAHVVEGADEVRVRLTDRREFKGKVLGADKATDIAVVKIDARDLPVVKLGDP
                     AKIRVGEWVLAIGSPFGFENTVTAGIVSATSRSLPDGTYVPFIQTDAAVNPGNSGGPL
                     FNLKGEVIGINSQIYSRSGGFQGIAFAVPIDVASNVKQQLVAHGSVQRGRLGVSIQEV
                     SQSLAQSF"
BASE COUNT          411 a          851 c          864 g          390 t
ORIGIN      
        1 atcgacagcg cccgcaggcg cctgcgctac gcgtggatcc tggtctcgat gctcgccgcg
       61 ctcggcgcct tcgccggcgc gagcgcgcaa gacaaaggcg cgcgcttcga cgacgcggtc
      121 atcacgcagc gcgttagcag cgcgctcgac gccgatccgg tcctgaggca aatgcatatc
      181 gccgtggaaa cccgcgatgg ggtggtccgt ctcaccggtt tcgtcgactc gacggcccag
      241 atcgaccggg ccgcggcgct cgcgcgccgc atcgagggag taagcgcggt gcgcaatgcg
      301 atccgcgtaa tcaaccggcc gtcgcgcgca tgactggcct gccgtaaaca gatagaggaa
      361 acgccatgaa ccttcccaag ctgccaatcg aaaccaaacc tttcttgtgg ggcgcggtag
      421 cgggcgcgat tacgctcgcc atcgtcggat tcacctgggg cggttgggtg accagcacca
      481 cggtcgagcg ccttgccggc gcccgcgcgg atgcggcgac ggtcgccgcg ctcaccccga
      541 tttgcgtgac acagttccag gcgagctcca gggcgcctgc cagcctcgcg gcgctgaagg
      601 ggatccagag ctgggagcag gccgactacg tgagtaaagg cggctgggcg acgatgcccg
      661 gcgacaacgc cgctccgagc cgcgaggtcg ccgccgcctg cgcagcggca ctgatcaagc
      721 acgcttcgta attccggggt cagaccccaa cgggaaaatc ggggtcagac cccgattttt
      781 ttctcgaagc ctcgcacgag ggccgctcgg gccgtcgccg cctgcatcag gagcgagaag
      841 tccgagccgc tcaggatgag ctgcacgccc atcgcgatgt ggcgctcgag gagctcgggc
      901 gtatacatgc cgcccatgcc ggggaacttg ccgtgcttgc ggcaggcggc gattaccttc
      961 ctgtaggcct cggcgacgcg cgggtcgttg aactggcccg ggatgcccat ctcgaagcac
     1021 aggtcgttgg tgccgatgag gagcgcgtcc acgcctttca ccgcggcgat ctcttcgcag
     1081 ttcgccacgc cctgcggcga ctcgatcatc accacgacga gcgtctcttc gttcacgagc
     1141 cgcgccgcct cgcccaccgg catcgaggcg aagcccagtt gcgcgagcgc gccgcccatg
     1201 gagcgcttgc cgatgggcgg atagcggcag gcgtttgcca cgcgctgcgc gtcctggctc
     1261 gaatcgacat gaggaaagac gatgccctgg gcgccgttgt cgagcatgcg cgaggcgtgc
     1321 cagtgctcga agcccgcgac gcgcacgatc ggcgtcacgc cggcgccgag cgcggcggtg
     1381 gagagctgcg cggcgtcgtc gccgtccatc gagctgtgct cgccgtcgat gaatagccag
     1441 tcgaagcccg cggtctttgc catggtggcg atgccgacgg tgcgcgcctg gttgacgccc
     1501 aggccgatgg cgagcttgcc ggcgcgcagc tggcgcaggg tgtggttcgg aacggtagcc
     1561 atggatgaag cgtagaacaa ttcgttacat cccgggcgtt ccgtttcgga aactcccggg
     1621 ccgcgcgcgg gtctgtcatg caacgccaag gagtcatcaa tcccatgaaa gcaaaagtgg
     1681 tttccgtggc cgtgcttgcc gccgccgtga tcggcacgag tgcgggcatt tcgcattttc
     1741 ccgtgcgcgc cgcgagtccc gccgccgcgg tgcagacgct tccggacttc gccgcgatcg
     1801 ccgaggcgaa caaggcggcg gtggtgaaca tcacttcctc ggcggcgccg aagccgggcg
     1861 cggacgcgcc cgaaggcctc gagaacctgc cggaagagct ccgccgcttc ctgccgcaga
     1921 tcccgccgca gccgcgccag ggcatgggct cgggcttcat cgtcgagtcc gacggcacca
     1981 tcctcaccaa cgcgcacgtc gtcgagggcg cggatgaagt gcgcgtgcgc ctcaccgacc
     2041 ggcgcgagtt caagggcaag gtgctcggcg cggacaaggc gaccgacatc gcggtggtga
     2101 agatcgacgc cagggacctg ccggtggtga agctcggcga cccggcgaag atccgggtgg
     2161 gcgaatgggt gctcgccatc ggctcgccgt tcggcttcga gaacacggtg accgccggca
     2221 tcgtcagcgc cacctcgcgc tcgctgcccg acggcaccta cgtgcctttc atccagaccg
     2281 acgccgcggt gaacccggga aactcgggcg ggccgctgtt caacctgaag ggcgaagtga
     2341 tcggcatcaa ctcgcagatc tactcgcgca gcggcggctt ccagggcatc gcgttcgcgg
     2401 tcccgatcga cgtcgccagc aacgtcaagc agcagctggt ggcgcacggc agcgtgcagc
     2461 gcggccgcct cggcgtgtcg atccaggaag tgtcgcagtc gctggcgcag tccttc
//
LOCUS       DBNGSG010000020         2404 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3401552, whole genome shotgun sequence.
ACCESSION   DBNGSG010000020 DBNGSG010000000
VERSION     DBNGSG010000020.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3401552"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="AC158Q_00440"
     CDS             <1..583
                     /locus_tag="AC158Q_00440"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016693.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3338264.1"
                     /translation="MLREWTSVGLVLPIDAVKSVKGGQAAWENHLLQQVQALPRLAKT
                     LDGARLSPGSVKVIRDYSYNSTQVTGPGFFLIGDAAGFIDPIFSVGIMLAMYSARTSA
                     WAIDRIFRYPDRAAEHQSIYASQLRSRMELSRALALPQYEIGDRATRDAKRGMQFTGT
                     QGRGLIRAASKLTERSLHYHALVEDPAKSPGHS"
     gene            587..1546
                     /locus_tag="AC158Q_00445"
     CDS             587..1546
                     /locus_tag="AC158Q_00445"
                     /inference="COORDINATES: protein motif:HMM:NF015251.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family acyltransferase"
                     /protein_id="HIR3338265.1"
                     /translation="MPTEPETTQKKAVQHGPSRARSGDELRENFFSLLLRSYRIAEKQ
                     GKGLAVYHSCADMVYRLCGERWAQHTIFPEESALVRRFLGEAGLARADADAALQLNLM
                     ANPWAFTWTQVLQELDAPRFASVCSASGEDLLRSALAQGRGVVCAHYHTLFAPLFWTW
                     LEHHGIAPGVLIREWVKSRPPAEAGDPKTQALEGARELKSSLDTLRRGGIAHVMADGY
                     AGNRKAVLPFCNRKRGFETTFSDLALMSGAPILTVVARFGADGRVRIEIGPALSNDPA
                     LDRVARTERLLQQFLAHLHRHWLEHPADISWFQMKRHLALPPA"
     gene            1578..>2404
                     /locus_tag="AC158Q_00450"
     CDS             1578..>2404
                     /locus_tag="AC158Q_00450"
                     /inference="COORDINATES: protein motif:HMM:NF022818.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3179 domain-containing (seleno)protein"
                     /protein_id="HIR3338266.1"
                     /translation="MPHDLLPDPPQRRPRPSGEPGQDIVRRKRRDEQGGLPPWYAPLP
                     APRWVRSEEATHMRPEDPVLCLALNGGAWALPWWVMKNHHVANLELNGKAVLVTLCEM
                     CTSSGAFDPVIDGRRRLFRLEGIYNGTIMPTDDETGSLWTGFTGESFEGPLKGTVMPR
                     LPLVQCTWEEWSGLHPDTLVADGEGEPRDGHGEGHTPGSPALFPGMRGLLSNVDQRLH
                     HYELVLGVFAGGAARCYPLSALALHGPALNDALGGEEIAVFCLPGSWIAIAYSRRVDG
                     "
BASE COUNT          408 a          802 c          814 g          380 t
ORIGIN      
        1 catgctgcgc gaatggacga gcgtgggact ggtcctgccg atcgatgccg tgaaatccgt
       61 gaaaggcggc caggcggcct gggagaatca cctgctgcaa caggtgcaag cgctgccgcg
      121 gctggcaaag acgctggacg gtgcgaggct gagcccgggc tcggtgaaag tcatccgcga
      181 ttattcctac aattcgaccc aggtcaccgg ccccggcttc ttcctcatcg gcgacgcggc
      241 cggcttcatc gacccgatct tttcagtggg aatcatgctc gccatgtatt cggcacggac
      301 gtcggcctgg gccatagacc gcattttccg gtacccggac cgcgccgccg agcatcaatc
      361 catctacgcg agccaattgc gttcgcgcat ggaactgtcg cgcgcgctgg ccctgccgca
      421 atacgagatc ggagaccggg ccacccgcga cgcgaaaaga ggcatgcagt tcacgggcac
      481 gcagggacgg ggcctgatcc gggcggcgtc gaagctgact gaacgatccc tgcattacca
      541 cgcgctggtc gaggatccgg ccaaatcccc gggacactcg taactcttgc ctaccgagcc
      601 cgaaaccacg caaaaaaagg cggtgcagca cgggccgtcc cgcgcaagaa gcggggacga
      661 gctgcgcgag aatttcttca gcctgctcct gcgcagctac cgcatcgccg agaaacaggg
      721 caagggcctg gccgtctacc attcgtgcgc ggacatggtc taccggttgt gcggggagcg
      781 ctgggcgcag cacacgatct ttccggaaga gtccgcgctc gtacgccgtt tcctgggcga
      841 agccggcctg gcgcgagccg atgccgacgc agcgctgcag ctcaacctga tggccaatcc
      901 ctgggccttc acctggaccc aggtcctgca ggaactcgac gcgccgcgct tcgcatccgt
      961 gtgcagcgcc tccggggaag acctgctgcg ctcggccctc gcccagggac gcggcgtggt
     1021 ctgcgcgcac taccacacct tgtttgcgcc gctgttctgg acctggctcg aacaccacgg
     1081 cattgcgccc ggcgtcctga tccgggaatg ggtcaagtcg aggccgccgg cagaagccgg
     1141 cgacccgaag acccaggcgc tggaaggcgc gcgcgagctg aagtcgtccc tggacacctt
     1201 gcggcggggc ggcatcgcgc acgtcatggc cgacggttac gcgggcaatc gcaaggccgt
     1261 gctgcccttc tgcaaccgca agcgcgggtt cgagacgact ttttccgatc ttgcgctgat
     1321 gtcgggagcg ccgattctca cggtcgtcgc gaggttcggc gccgacggac gcgtccggat
     1381 cgaaatcggg ccggcgctga gcaatgaccc ggcgctggac cgggttgccc gcaccgagcg
     1441 gctgctgcaa cagttcctgg cgcacctgca ccggcattgg ctggaacatc ccgccgacat
     1501 atcgtggttc cagatgaaac ggcacctcgc gctgccgccg gcctagcggc cccgcatcac
     1561 gaatcgcgcc cgaaaccatg cctcacgact tgcttcccga cccgccgcaa cgcaggcccc
     1621 ggccgtccgg tgagcccggg caggacatcg tgcggcgcaa gcggcgagac gagcaaggcg
     1681 ggctgccgcc ctggtacgcc ccgttgccgg ctccgcgctg ggtccgtagc gaggaagcaa
     1741 cgcacatgcg cccggaagac cccgtgctgt gcctggcgct gaacggcggc gcctgggcat
     1801 tgccgtggtg ggtcatgaaa aaccatcacg tggcgaacct cgagttgaac ggcaaggcgg
     1861 tgctggtgac actgtgcgag atgtgcacca gctccggcgc gttcgatccg gtcatcgacg
     1921 gacggcgccg cctgttccgg ctggagggca tctacaacgg cacgatcatg ccgacggatg
     1981 atgaaaccgg cagcctgtgg accggattca cgggcgaatc gttcgaggga ccgctgaagg
     2041 gaacggtcat gccgcgcctg ccgctggtcc agtgcacctg ggaagagtgg agcggcctgc
     2101 atcccgacac gctggttgcc gatggcgagg gcgagccgcg cgacggtcac ggtgagggac
     2161 atacgcccgg cagcccggcg ctgtttccgg gcatgcgcgg cctgctgtcg aacgtggacc
     2221 agcgcctgca tcattacgaa ctggtgctcg gtgtgttcgc cggcggcgcg gcccgctgct
     2281 acccgttgtc ggcgctggcc ctgcacgggc ccgcgctgaa cgatgcgctc ggcggcgagg
     2341 agatcgccgt gttctgcctg cccgggagct ggatagccat cgcgtattcg cggcgggtgg
     2401 acgg
//
LOCUS       DBNGSG010000021         3832 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3062534, whole genome shotgun sequence.
ACCESSION   DBNGSG010000021 DBNGSG010000000
VERSION     DBNGSG010000021.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3832)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3062534"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="AC158Q_00455"
     CDS             complement(<1..558)
                     /locus_tag="AC158Q_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338267.1"
                     /translation="MTTGISRTIRAGSPAGRTQAAGAAAALLAAMIGLTGSATGAPAV
                     GDTYSYQLVNGYNKEVRGRVHYQVDRVEADRVTVSVNSDSAEAGRAHTETYTKEGNWL
                     RNLVESHGVPVEYVFATAYPAYVFPLEPGKSWSVRVKATVPILGARRSVRVDAWVIGA
                     ERIRVPAGEFDTLKIRRAVYPGDDSI"
     gene            complement(675..1676)
                     /locus_tag="AC158Q_00460"
     CDS             complement(675..1676)
                     /locus_tag="AC158Q_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338268.1"
                     /translation="MGHQVTRAWLLLLLALVPAGAAAQRSPSPAAAEDRNRATDDFDL
                     LPGAGGSFDFIGTRDSDGFETRRTRIGALVHHNSAYDFSGVAAGTDYYRQDSWSERGY
                     SLWGVLEKRDRAAAAGISAHIGLLHVNGFDHLLADAVWNHRFSAQTGSEVIFQRDVVE
                     TRAALDNGVNQNFLGASVDHAFSNRLTAVALAGAQQFSDDNNRGHLRGWIIYTLAPEY
                     GLAVQLRARGYENSRQGSPFYFNPERYENVDIGLRLNKRFGAWRVRAFLAAGEERIDR
                     GVANPTGFAQLHVDRVLAGGIRIGLRYAYSHAAGEDRGNADGTYSWRYLRLFVVAPF"
     gene            complement(1661..3016)
                     /locus_tag="AC158Q_00465"
     CDS             complement(1661..3016)
                     /locus_tag="AC158Q_00465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3338269.1"
                     /translation="MNPLVSAGPWPSDAETRHQRSDSNAALRFLVAAAAGSAWFGLSL
                     WLALPWIDELGGIVGPALAWFLIFGVALAPGFMNAFVVASLLMIPSVSRNEPQHLPPL
                     TILIAAYNEAANIGATLRSIAAQRYRGAFEVIVIDDGSTDDTAKMTATGGYPWMRLLA
                     QPANRGKAAALNRGLAEARHELIVTLDADSCLHPDALRLLVMEYASGTNDMRAVAGAV
                     FVSNPHASWITRLQHWDYFNGIAAIKRVQGSYGGTLVAQGAFSLYERSALREIGGWPA
                     CVGEDIVVTWAIIKRGGRVGYAPDAACFTRVPETLRGLSHQRIRWGRGMIEAFRYHPD
                     ILVMRRLSAFMASWNLLFPCIDFAFTFGFIPGLVLALFGKYWLVGPATLAVIPSAILM
                     GAVMQRLSAQVFTALDLQIRRDAIGFMGYTLGYNLILQPSSLRGYFAEVLGLPKTWGT
                     K"
     gene            complement(3048..>3832)
                     /locus_tag="AC158Q_00470"
     CDS             complement(3048..>3832)
                     /locus_tag="AC158Q_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HIR3338270.1"
                     /translation="LRMLRDGKVALALSQGDAALAAYEGKGNFAEDGPHLTLRAIGSL
                     YPEPVHVIVRADSPIVSMANLAGRRVAVGPAGSASRTTALRVLEAHGLGAKQLGGMPD
                     LPLGEALVALRAREVDAVIQVIGAPADSVRDALVAIPLRLVPLSERAVTALVASRSGY
                     FAHTIPHGAYSGQRQDVSTIATAAILLAGSDLSDTEVGALTRYVFGERRDFAARGSAQ
                     GTQVSAANARLGLSVPLHVAAAKALAGLAPAAEQVRKTPASK"
BASE COUNT          660 a         1297 c         1253 g          622 t
ORIGIN      
        1 tatggaatcg tctcccggat agacggcacg cctgatcttg agggtgtcga attcacccgc
       61 cggcacgcgg atgcgctcgg ctccgatcac ccaggcatcc acgcgcacgc ttctgcgcgc
      121 gcccaggatc ggcacggttg ccttgacgcg caccgaccag gatttccccg gttccagcgg
      181 aaacacgtag gcggggtagg ccgtggcaaa cacgtactcc accggcacgc cgtggctctc
      241 gaccaggttg cgcagccagt tgccttcctt cgtgtaggtt tcggtgtgcg cccgaccggc
      301 ttcggcacta tcggagttga ccgaaacggt gacgcgatcc gcttccacgc ggtcgacctg
      361 gtaatgcacc cgcccgcgga cttccttgtt gtagccgttc acgagttgat aactgtaggt
      421 gtcgcccacg gcgggcgccc cggtcgcgga gcccgtcagc ccgatcatcg cggcaaggag
      481 cgctgcggcg gcgcccgctg cctgcgtccg accggccggc gagccggcac gaatcgttcg
      541 agagatcccc gtagtcatga tggctgccat ttcgctggct gatgatgggg tgaacaccct
      601 cgcttgcccg acccttccgc tttccagctg cagcccgtct gttgctggtc ggcgcgcgcg
      661 ccttatttcc cggcctagaa cggcgcaacc acgaacaggc gcagatagcg ccagctatag
      721 gtgccgtcag cattgccgcg atcctctccc gcggcatggg agtaggcgta gcgcaagccg
      781 atccggatac ctccggccag gacgcggtcg acatgaagtt gcgcgaagcc cgtcggattg
      841 gcgacgccgc ggtcgattcg ttcctcgccg gcagccagaa acgcgcgcac ccgccacgcc
      901 ccgaagcgct tgttcaatct caggcctatg tccacgttct cataccgctc gggattgaag
      961 tagaacgggc ttccctgccg gctgttttcg tagccccgcg cccgcaattg caccgcgagc
     1021 ccgtattccg gcgcgagcgt ataaatgatc cagccgcgga gatgaccgcg gttgttgtcg
     1081 tcggagaact gctgggcgcc ggccagcgcg acggcggtca gccggttcga aaacgcatga
     1141 tccacgctcg cgcccagaaa attctggttc acgccattgt ccagcgcggc tctcgtctca
     1201 acgacatcgc gctggaatat cacttccgag ccggtttgcg cggaaaaacg gtggttccag
     1261 acggcatccg ccagcaggtg gtcaaaacca ttgacgtgca gcaggccgat gtgcgccgag
     1321 attcccgcgg cggccgcgcg atcccgcttt tccagcacgc cccacagcga gtacccgcgc
     1381 tcggaccacg aatcctgcct gtaatagtcg gtgccggcgg caaccccgga aaaatcataa
     1441 gcggaattgt gatgtaccag cgcgccgata cgcgtgcgcc gggtctcgaa tccgtctgaa
     1501 tcgcgggtgc cgatgaagtc gaaactgccg cccgcaccag gcaacaggtc gaagtcgtct
     1561 gtggcgcggt tccgatcctc ggcggcggcc ggggaaggcg accgctgcgc cgccgcgccg
     1621 gccgggacga gcgccagcag aagcaacagc caggcccggg tcacttggtg ccccatgtct
     1681 tgggcaggcc cagaacttcg gcgaagtagc cgcgcaggct cgagggctgc aggatcaggt
     1741 tgtagcccag ggtgtagccc atgaaaccga tcgcatcgcg tcgtatctgc aggtcgagcg
     1801 cggtgaatac ctgcgcgctc agccgctgca ttactgcccc catcaggatg gccgagggga
     1861 tcacggcgag ggtcgcgggc ccgaccagcc agtacttgcc gaacagcgcc agcaccagcc
     1921 cgggaatgaa gccgaaggta aacgcaaaat cgatgcaggg aaacaggagg ttccagctgg
     1981 ccatgaatgc cgagagccgg cgcatcacga ggatgtccgg gtgatagcgg aaggcttcga
     2041 tcatgccccg gccccatcgt atgcgctggt gcgacagtcc gcgcagcgtt tcgggaacgc
     2101 gcgtgaagca ggcggcgtcc ggtgcgtagc cgacgcgccc cccgcgcttg atgatggccc
     2161 aggtcaccac gatgtcttct ccgacacagg cgggccagcc gccgatttcc cgaagcgcgc
     2221 ttcgctcgta gagcgaaaag gccccctggg ccaccagcgt tccgccatag gagccctgca
     2281 cgcgcttgat ggcggcgatc ccgttgaagt aatcccagtg ctgcaggcgc gtgatccagc
     2341 ttgcgtgggg attgctcacg aacaccgcgc ccgccaccgc gcgcatgtca ttcgtgccgc
     2401 tggcgtattc catgaccagc agacgcagcg catccgggtg caggcaactg tccgcgtcga
     2461 gggtcacgat gagttcatgc cgcgcctcgg ccagcccccg gttcaacgcg gccgccttgc
     2521 cccggttggc aggctgcgcc aacagccgca tccacggata gcctcccgtg gcggtcattt
     2581 tcgcggtgtc atcggtcgag ccgtcgtcga tcacgatgac ctcgaatgcg ccccggtaac
     2641 gctgtgccgc gatcgagcgc agcgtcgcgc cgatgttggc tgcctcgttg taggccgcga
     2701 tcaggatggt gagcggggga aggtgctgcg gctcgttgcg gctgacggat ggaatcatca
     2761 agaggcttgc gaccacgaac gcattcatga atccgggcgc aagcgcaaca ccgaagatga
     2821 ggaaccaggc gagcgcaggc ccgacgattc cgcccagttc atctatccag ggcagggcga
     2881 gccacagcga cagtccgaac caggcgctgc cggcggcggc tgcgaccagg aagcgcagcg
     2941 cggcgttcga atccgaacgc tgatgcctcg tttcggcatc ggaaggccat ggtcccgcgg
     3001 agacaagtgg attcaagatt gctgcccccc tcttaccggg ggacttccta ttttgacgcg
     3061 ggcgtcttcc tgacctgctc ggcggcagga gcgagtcccg ccaatgcctt ggcggcggcg
     3121 acgtgcagcg gcaccgaaag acccagccgc gcgttcgccg cggagacctg cgtgccttgg
     3181 gcgctgccgc gcgccgcgaa atcgcgcctt tccccgaaca cgtagcgtgt gagcgcgccg
     3241 acctcggtgt ccgagagatc ggagccggcg agcaggatcg ccgccgtcgc gatggtgctc
     3301 acgtcttgcc tctgcccgga gtaggcgccg tgcgggatcg tatgggcgaa gtagccgctc
     3361 ctggaagcaa cgagcgctgt gaccgcgcgc tccgagagcg gcaccaggcg cagcggaatg
     3421 gcaaccagcg catcgcgcac gctgtcggcg ggcgcaccga tgacctggat gacggcatcc
     3481 acttcccggg cgcgcagggc gaccagcgct tcgccgagcg ggaggtccgg catcccgccg
     3541 agctgcttcg cgccgagccc gtgcgcctcc agcacgcgca gcgccgtggt ccgcgacgcc
     3601 gaacccgccg gccccaccgc aacgcggcgg cccgcgaggt tcgccattga gacgatcggg
     3661 ctgtctgccc tcacgatgac atgcacgggc tccgggtaaa ggctgccgat ggcgcgcagc
     3721 gtgaggtgcg gcccgtcttc cgcaaaattg cccttgcctt cgtacgccgc gagcgccgcg
     3781 tcgccctgcg acagggcgag cgccaccttg ccgtcgcgca gcatccgcag gt
//
LOCUS       DBNGSG010000022         5049 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3232613, whole genome shotgun sequence.
ACCESSION   DBNGSG010000022 DBNGSG010000000
VERSION     DBNGSG010000022.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5049)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5049)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3232613"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="AC158Q_00475"
     CDS             <1..428
                     /locus_tag="AC158Q_00475"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3338271.1"
                     /translation="WRHIVGIALDGTFFCTRAAVPHMLKNGWGRIVNISGVSHYAPSY
                     SGRAHVSAAKAGVEGFTRALAAEYAKKNITVNCVCPGRIGGDRSASSSDLPDPTLIPP
                     VGHLGVSEDIAGAVHYFCLPTSSFVTGHTMHVNGGQVLL"
     gene            663..2375
                     /locus_tag="AC158Q_00480"
     CDS             663..2375
                     /locus_tag="AC158Q_00480"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HIR3338272.1"
                     /translation="MADKSANVSQPKNYKLIVEKDVKIPLRDGTMLYADILRPDGGAG
                     GERFPAIMNISVYQKDKVWIPPADLEEKANPHMNWETVNPQWWCPRGYACVRIDSRGT
                     GKSPGKSDPSSYQEALDFYDAIEWVAKLPWCSGNIGTLGISYHASSQWRVANLNPPSL
                     KCIMPWEGRADQYRDQAYHGGIFSLGFIGNWWLTHTAHHLLGRPRSYNPDAFQNDMLW
                     NMMRNDLDSEWWRQCSARWERIKVPVYTVGNWGGYSLHLRGNTEGYMNAASRHKKLRI
                     HTGTHFHPFHSEEGRLDQLRWMDHWLKGRDTGILEEPPVKLEIRTGGSHKPYPFRAEN
                     EWPLARTQWTKAYLRIDREQGHEEMAVEGELAQAAPKTAGSVSYPASGMTSAGVASGS
                     SLSTTHGGGGRLGVSFETPAMKEPTEITGPLMMNLWVSSTSEDMDVFVTLRNIGPDGK
                     EVYEVGQHGQPVPLTKGWLRASHRKLDPGKSLPYRPFHPHDERRWLTPGEPVECQIEI
                     WPTCIVLGKGHKLRVDIQPRDGVGSAPYTHYHADYNAGATNTIYSGGDRQSYILLPII
                     PPKK"
     gene            complement(2611..3588)
                     /locus_tag="AC158Q_00485"
     CDS             complement(2611..3588)
                     /locus_tag="AC158Q_00485"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3338273.1"
                     /translation="MRTHKMLAAIAVPAMLAGPAVQAAQTAAYPDKPIRLIIGSAAGS
                     GPDIMARLIADHLFGVWGQRVVVDTRPGVAGIISAEQALRSERDGYTWMMLTSQLFIA
                     TSVYPDLKFSLDKDFDSVSLVGLVPWVVTVNPGLPAKSTSELIALARKSPGKLRYGSG
                     GPGSGEHFATVMFTHLAGIDMLHVPYKGVAGALIDAIANEIQMQFAVYPAAFPHVNSG
                     RLRAIAVSTAKRAPGLPNVPTIADTLPGFVNFGWYSIVAPKGIPAPILAKASAEIVKA
                     ARQPAFGERLKTLGIEIIAGGRKELDDFRRTERKRLTELVKATGISITK"
     gene            3687..>5049
                     /locus_tag="AC158Q_00490"
     CDS             3687..>5049
                     /locus_tag="AC158Q_00490"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HIR3338274.1"
                     /translation="MSDTRSETRDGMRIDWDVPIRMDDGIVLRADVYRPVAAGKYPAL
                     VSYGPYGKGLAFQEGYRTAWEIMARDNPDAVSGTTNQYQNWEVVDPEKWVPDGYACVR
                     VDSRGAGRSPGYLSHNDARENRDFHDCIEWAAAQPWCSGKVGLNGISYYASSQWRAAA
                     LQPPHLAAICVWEGWADYYRDSVRHGGIACTFRKHWQDMQVKTVQHGRGEHGPRSRVT
                     GELVCGPETLSPEELLKNRSDMWESVLKYPLAGPYYQERTADLSKVMVPLLSCANWGG
                     QGLHPRGNFEGYVRAASKQKWLEAHGGSHWAPFYTDYGVKLQKRFFDFFLKGRQNGWD
                     KQPRVTLQVRHPGEKFVQRHENEWPLARTRWTNFYLDPAARSLKLEPPASEGSITYDG
                     LGEGVTFNSAPMDKETEITGPSALKLWVSSATADADIFAVLRVFDPQGKEVVFQGALD
                     PHTP"
BASE COUNT          941 a         1614 c         1681 g          813 t
ORIGIN      
        1 aatggcggca catcgtcggc atcgcgctcg acggcacgtt cttctgcacc cgcgcggcgg
       61 tgccgcacat gctgaagaac ggctggggcc gcatcgtcaa tatcagcggc gtcagccact
      121 acgcgccgag ttacagcggg cgcgcgcatg tttcggcggc gaaagcgggg gtggagggtt
      181 ttacccgcgc gctggccgcg gagtacgcga agaagaacat caccgtgaac tgcgtttgtc
      241 cgggacggat cggcggcgac cgctccgcct cgtcaagcga cctgcccgat cccacgctga
      301 tcccgccggt cggccacctt ggcgtttccg aggacatcgc cggcgcggtg cattattttt
      361 gcctgcccac ctcgagcttc gtcacggggc atacgatgca cgtcaatgga ggacaagtcc
      421 tcctctgagg gaggagtgtc gagaaatggg tgtcgagtga atcaagtgaa taaggaccaa
      481 ggaacctctg cataagtcat cctcgcgaaa gcggggatcc agtaaccatc ggttttgcct
      541 ggattcccgc cttcgcggga atgacgaaaa acgacttttt cagaacttct ctaactgagc
      601 tttacccctc gacacccgac acccaacact cgaatcacga ttcacgcatt cagggagaaa
      661 aaatggccga caagtccgcc aacgtatcgc agccgaaaaa ctacaagctg atcgtcgaga
      721 aggacgtgaa gattccgctg cgggacggga cgatgctgta cgccgacatc ctgcgccccg
      781 atgggggagc cgggggcgag cgctttcccg cgatcatgaa catcagcgtc taccagaagg
      841 acaaggtatg gatcccgccg gcggacctcg aggagaaggc caatccccac atgaactggg
      901 agacggtcaa cccgcagtgg tggtgcccgc gcggctacgc ctgcgtgcgc atcgattcgc
      961 gcggcaccgg caagtcgccc ggcaagtccg atcccagctc gtaccaggag gcgctcgact
     1021 tctacgatgc gatcgagtgg gtcgcgaagc tgccctggtg ctcgggcaac atcggcacgc
     1081 tcggcatctc ctatcacgcg agctcgcagt ggcgcgtagc caatctcaat ccgccgtcgc
     1141 tgaagtgcat catgccgtgg gaagggcgcg ccgaccagta ccgcgaccag gcctatcacg
     1201 gcggcatctt ttcgctcggc ttcatcggca attggtggct cacgcacacc gcgcaccacc
     1261 tgctgggccg cccgcgcagc tacaaccccg acgcgtttca gaacgacatg ctgtggaaca
     1321 tgatgcgcaa cgacctcgat tccgagtggt ggcggcagtg cagcgcgcgc tgggagcgca
     1381 tcaaggtgcc ggtctacacc gtcggcaact ggggcggcta ttcgctgcac ctgcgcggca
     1441 ataccgaggg ttacatgaac gccgcgtccc ggcacaagaa gctgcgcatt cacaccggca
     1501 cccacttcca cccgttccac tcggaggagg gacgccttga ccagctgcgc tggatggatc
     1561 actggctgaa gggcagggac accggcattc tggaggagcc gccggtcaag ctcgagatcc
     1621 gcaccggggg cagccacaag ccgtacccgt tccgcgccga gaacgaatgg ccgctcgcgc
     1681 gcacgcaatg gaccaaggcc tatctccgca tagaccgcga gcagggccat gaggaaatgg
     1741 ctgtcgaagg ggaactcgcg caagcggcgc cgaagaccgc cggaagcgtg agctacccgg
     1801 cgagcggcat gacttccgcg ggcgtggcgt cgggttcgtc cctgtcgacg acccatggcg
     1861 gtggcgggcg gctgggtgtc tccttcgaga ccccggccat gaaggagccg accgagatca
     1921 ccggcccgct gatgatgaac ctgtgggtgt cctccacgtc cgaggacatg gacgtcttcg
     1981 tcacgctgcg caacatcggc cccgacggaa aggaggttta cgaggtcggc cagcacggcc
     2041 agccggtgcc gctcacaaag ggctggctgc gcgcctcgca ccgcaagctc gatcccggga
     2101 agtcgctgcc gtaccgcccg ttccatccgc acgacgagcg ccgctggctc accccgggcg
     2161 agccggtcga gtgccagatc gaaatctggc ccacttgcat cgtgctcgga aaggggcaca
     2221 agctgcgcgt ggacatccag ccgcgcgacg gcgtcgggtc ggcgccgtac acgcactacc
     2281 acgccgacta caacgcgggg gccaccaaca ccatctactc gggcggcgac cggcagtcct
     2341 atatcctgct gccgattatt ccgccgaaga agtagcgcgt cgaggggtgg aagaggggcc
     2401 ccgaacttgt tcggggatct gacccccggt ggcgcgcaag cggccgacgc gatgtgaaag
     2461 aaatttgcgc caagctgtcg gaagccgctg tgatgagtga tgggccgcgt gatgaacggc
     2521 ggaagccgtc cgttcgatca cgcggagcta aaccggcgat cagggcacgg gagccgacgc
     2581 tcccttaacc ctgggcgtcc ggctttgacg tcacttggta atggagatgc cggtggcctt
     2641 caccagctcg gtgaggcgct tgcgctcggt ccggcggaag tcatcgagtt ccttgcggcc
     2701 gccggcgatg atctctatgc ccagggtctt cagccgttcg ccgaacgcgg gctggcgcgc
     2761 cgccttgacg atctcggcgc tcgccttggc gagtatcggc gcggggatgc ccttgggcgc
     2821 cacgatgctg taccagccga agttcacgaa gcctggcaag gtgtccgcga tcgtcgggac
     2881 gttgggcagc cccggcgcgc gcttcgcggt gcttaccgcg atcgcccgta gtctgccgga
     2941 gttgacgtgg gggaacgcgg cggggtagac ggcgaattgc atctggattt cgttggcgat
     3001 cgcgtcgatc agcgcccccg cgacgccctt gtagggcacg tgcagcatgt cgatgccggc
     3061 caggtgtgtg aacatgacgg tagcgaaatg ctcgccgctg ccggggccgc cggagccgta
     3121 gcgcagcttg cccggcgact tcctggcgag cgcaatcagc tcggacgtgg actttgccgg
     3181 gagccccggg ttgaccgtca ccacccacgg caccagcccg acgagggaga ccgagtcgaa
     3241 atccttgtcg agactgaact tgaggtcggg atagaccgag gtggcgatga agagctgcga
     3301 agtcagcatc atccacgtgt agccgtcacg ctccgagcgc agcgcctgct ccgcgctgat
     3361 gatgccagcc acccccggcc gggtatccac gaccactctc tggccccaca caccgaacag
     3421 gtgatcggca atgagcctcg ccatgatgtc cggccccgac cccgccgcgc tgccgatgat
     3481 gagccggatc ggcttgtccg ggtaggcggc ggtctgcgcc gcctgtaccg ccgggcccgc
     3541 gagcatcgcc ggtactgcga ttgctgccag cattttatga gtgcgcatct cgaccctcct
     3601 cgaggtttgc gtgcctaggt tagtggccac ccgccgccag gtcaacttcc gcatttcagg
     3661 caaaatacga agaaccaggg ggggagatga gcgacaccag gagcgagact cgcgacggca
     3721 tgcgcatcga ctgggacgtg ccgatccgga tggacgacgg catcgtgctg cgagcggacg
     3781 tgtaccggcc ggtggcggcg gggaaatacc cggcgctggt gagttacggg ccgtacggca
     3841 aggggctcgc gttccaggag ggctacagga cggcgtggga gatcatggcg cgcgacaatc
     3901 ccgacgccgt ttccggcacc accaaccagt accagaactg ggaagtcgtg gacccggaga
     3961 agtgggtgcc ggacggctac gcctgcgtgc gcgtggattc gcgcggcgcg gggcgctcgc
     4021 cgggttatct cagccacaac gacgcgcgcg agaaccgcga cttccacgac tgcatcgagt
     4081 gggcggcggc gcaaccgtgg tgcagcggga aagtcggcct gaacggcatt tcctactacg
     4141 ccagcagcca gtggcgcgcg gcggcgctgc agccgcctca cctcgcggcg atctgcgtgt
     4201 gggaaggctg ggcggactac taccgcgact cggtccgcca cggcggcatt gcctgcacct
     4261 tccgcaagca ctggcaggac atgcaggtga agacggtgca gcacgggcgc ggcgagcacg
     4321 ggccgaggag ccgcgtgacc ggcgagctgg tgtgcgggcc ggagacgctc tcgccggaag
     4381 agctgctgaa aaaccgctcg gatatgtggg aaagcgtcct caagtacccc ctcgccggac
     4441 cctattacca ggaacgcacg gccgacctgt cgaaggtcat ggtgccgctg ctctcttgcg
     4501 ccaactgggg tgggcagggc ctgcacccgc gcggaaactt cgagggttac gtgcgcgccg
     4561 cgtcgaagca gaagtggctg gaagcgcacg gcggctcgca ctgggcgccg ttctacacag
     4621 actacggcgt caagctgcag aagcggttct tcgatttttt cctgaagggc aggcagaacg
     4681 gctgggacaa gcagccgcgc gtcacgctcc aggtgcgcca tcccggtgaa aaattcgtcc
     4741 agcgccatga gaatgaatgg ccgctcgcgc gtacccggtg gacgaatttt tatcttgatc
     4801 cggcggcgcg cagtctgaag ctcgaaccgc ccgcttccga gggcagcatc acctacgacg
     4861 gacttggcga aggcgtcacg ttcaactctg cgccgatgga taaggagact gaaatcaccg
     4921 gcccgtcggc actgaagctg tgggtctcgt cggccaccgc cgatgcggac atcttcgccg
     4981 tgctgcgcgt gttcgatccg caggggaagg aggtcgtgtt ccagggcgcg ctcgacccgc
     5041 acacgccgg
//
LOCUS       DBNGSG010000023         4878 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_855993, whole genome shotgun sequence.
ACCESSION   DBNGSG010000023 DBNGSG010000000
VERSION     DBNGSG010000023.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4878)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4878
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_855993"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="AC158Q_00495"
     CDS             <1..410
                     /locus_tag="AC158Q_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HIR3338275.1"
                     /translation="AMGEFPEGSEIIPNPYNRIPGFSYRRHYFLPGFPEMAWPMMAWV
                     LDTHYAQLFAPGSVAESSIIVREAGESQLIELMKDCQARYRGIKVFSLPKVQPERYIE
                     LGVRGHPGEVTPAIAALKQGVSDLGFPWSDAPR"
     gene            complement(581..1123)
                     /locus_tag="AC158Q_00500"
     CDS             complement(581..1123)
                     /locus_tag="AC158Q_00500"
                     /inference="COORDINATES: protein motif:HMM:NF020918.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HIR3338276.1"
                     /translation="MYQTPEQLVAINKANLEVAMKFAGVALQGAERILDLQLKAAKTA
                     FADSVENAKTIAAVKDIKELASLKDSLAQPSIEKATAYAKNVYDVTTATQAQFGALVE
                     EQVSEFNKQVVTALDKMVKTAPAGSEVGIAAFKSAIAAVNSGYENISKVAKQFADATQ
                     SNIEAVAKQASNGAKKAKKS"
     gene            1340..1678
                     /locus_tag="AC158Q_00505"
     CDS             1340..1678
                     /locus_tag="AC158Q_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HIR3338277.1"
                     /translation="MTTLFGRIIRREIPADIVYEDELCLAFRDVNPQAPTHVLVIPKK
                     EIARLSDAGADDQALLGHLLLAAGKIARSLGVGDAFRLVVNNGAEAGQSVFHLHLHIL
                     AGRKLRWPPG"
     gene            1709..2680
                     /locus_tag="AC158Q_00510"
     CDS             1709..2680
                     /locus_tag="AC158Q_00510"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3338278.1"
                     /translation="MKNRHCSTVVAGVIGFMLVAGGAFAQQYPVKPVRIVVPTSPGGG
                     NDFVARAAGQRLGDRLGQQFIVDNRPGAGGSIGTTIAAKAAPDGYTLLLGFVGQLAMY
                     PHVEKTEYDSQNDFVGVSLIASSYHVMAVHPSLPVRSVKELIAFAKARPGELYYSSGN
                     IWTPTHLVPELFNAATGTRLVPIHYKGSGPSVVGVLTGETQVIMSSVTAVMPHVRSKR
                     LVALAVTSPARTPVAPEVPTLVELGVRGVEAPSWYAIVAPAATARPIVDKLHGELAAM
                     STQPDFQALFRKQALEPTSKTPEEFAVFLRGEYDKWGKIIKSLRSQK"
     gene            complement(2772..3866)
                     /locus_tag="AC158Q_00515"
     CDS             complement(2772..3866)
                     /locus_tag="AC158Q_00515"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3338279.1"
                     /translation="MKREGKQKELGVAVIGAGRIGTLRARLAARHPAVGFLAIADLDP
                     RRAKALADVTDADFHTGSNFAAIERPEVSAVFVSTPESLHSAPVRRALELGKPVLCEK
                     PIALSLKDADDILETLKSTGGELRIGYSRRYKECFLRAKEQMIHGRLGKVVGGLARVY
                     NSRAQTFAILKRDPHATPVLDVLTYYVDLMCWFLEGNRPVEVVARGQHGIFKEAGYSA
                     HDATWAIVTFADGAVVNFGISYALPAQFPTQGQSDRVELLGTEGTMLIDDDHKEHLLF
                     TEKGVPHPYVPGHSLHMAFLGSNSAGDWALGDFWGSLGNETRAWLDHLVTGNPTAHTT
                     PEQARVNLETTLAIERAVETGKPVRLPLET"
     gene            complement(3863..4489)
                     /locus_tag="AC158Q_00520"
     CDS             complement(3863..4489)
                     /locus_tag="AC158Q_00520"
                     /inference="COORDINATES: protein motif:HMM:NF014664.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3338280.1"
                     /translation="MARIPPASAEQFAQVADHMERWKKTKGYPPNSWLAMARKPRVFR
                     AYRDLHTAVMMDDGEVPKALKFMIACVVSTAAGDPYCAAHNAENAVHIAGVPVGKVEA
                     LPHFRSSTLFTPGERAALELASAAGSCPPRVTDAHFAELGKYYGQDAAAEVVAVISLL
                     GWLNRWNITLATRIEAEALAFAEKHLAPSGWAPGVHAVPPQGRQEVNG"
     gene            complement(4527..>4878)
                     /locus_tag="AC158Q_00525"
     CDS             complement(4527..>4878)
                     /locus_tag="AC158Q_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3338281.1"
                     /translation="LLPQTRSMIGARQLAVMKPGAVLIQASRGGVVNETALAAHLSSG
                     RLGGAAVEVYSVEPPDTDNPLFSLEGEAAHRVLLTPHVAGVTLQASATLFREAWANVE
                     RVLVRGEAPLNRVY"
BASE COUNT          824 a         1535 c         1608 g          911 t
ORIGIN      
        1 tcgccatggg cgagttcccg gagggctccg agatcattcc gaacccctac aaccgcattc
       61 ccggcttcag ctaccggcgc cattacttcc tgccgggctt tcccgaaatg gcctggccga
      121 tgatggcctg ggtgctcgac acccactacg cgcagctatt cgcgccgggc agcgtcgcgg
      181 aatcctcgat catcgtgcgc gaagcgggcg agagccagct gatcgagctg atgaaggact
      241 gccaggcgcg ctaccggggc atcaaggtat tcagcctgcc gaaagtgcag cccgagcgct
      301 acatcgagct cggcgtgcgc ggccatcccg gggaagtcac acctgccatc gcggcgctca
      361 agcagggtgt cagcgatctc ggttttccct ggagcgatgc accccgctag caggctcccg
      421 gccgcggagt gcaagcaaaa agccccggga atcccggggc ttttcttgat gctgcttccc
      481 gcgcgttgaa cggcggcttt ctgtccgttc gaacgcgcgg agttaatccg gcgcttcagg
      541 acacggaagc cgtggcttcc atgcccttaa gcgtccggga ttaacttttc ttcgcctttt
      601 tcgcgccgtt cgacgcctgc ttggcaaccg cttcgatgtt cgactgggtc gcgtccgcga
      661 actgcttggc gactttcgag atgttctcgt aaccggagtt cacggcggcg atggccgact
      721 tgaatgcggc gattccgact tcggagcccg ccggggcggt cttcaccatc ttgtccagcg
      781 cggtcacgac ctgcttgttg aactcggata cctgctcttc gacgagggcg ccgaattgag
      841 cctgggtcgc cgtggtcacg tcatagacgt tcttcgcgta ggcagtcgcc ttctcgatgg
      901 agggttgcgc caggctgtcc ttcagcgagg ccagttcctt gatgtccttc acggcagcga
      961 tggtcttcgc gttctccacc gagtcggcga aggcggtctt ggcggccttc agttggaggt
     1021 cgagaatgcg ctcggcgccc tggagggcta cgccggcaaa cttcattgcg acttcgaggt
     1081 tggccttgtt gatggcaacc agttgttcag gggtttgata catacagttc tccttgaaaa
     1141 tcactacatt aatcaaataa tggtgcaccg cacaaaacgt attatatgct tcaaatctgg
     1201 agagtcaatg ggtactggct aaaaatatat tgcaatgcag taagtccatg aaagcaagcg
     1261 aatactcgcg aaaccctttg acttggggct atccggttta taggatggga aaaattgtta
     1321 tgaatcaagg agaagcgctg tgaccaccct gttcggcaga atcattcgcc gcgagatacc
     1381 ggcagacatc gtctacgagg acgaactctg cctggcgttc cgggacgtca atccccaggc
     1441 gccgacccac gtgctggtca tacccaagaa ggagatcgcc aggttgtccg atgccggtgc
     1501 ggacgatcaa gccctgctcg gacacctcct gctggctgcc ggcaagatcg cgcgcagcct
     1561 cggcgtgggc gacgccttcc gcctcgtggt caacaacggc gcggaagccg ggcaaagcgt
     1621 atttcacctg caccttcaca tcctcgccgg gcgcaagctg cgctggccgc caggttaagt
     1681 cacaatgatg aacgaggagg gataaaccat gaaaaacagg cattgcagta cggtggtcgc
     1741 cggcgtgatc ggtttcatgc tcgtagccgg cggcgcgttc gcgcaacagt atcccgtcaa
     1801 gccggtgagg atcgtggttc cgacttcacc gggcggaggc aacgatttcg tcgcgcgggc
     1861 cgccgggcag cggctcggcg accggcttgg tcagcagttc atcgtggaca atcgcccggg
     1921 cgcgggcggc agcatcggca ccaccatcgc ggcgaaagcg gcgcccgacg gttacacgct
     1981 gctgctcggt ttcgtcggcc agctcgcaat gtacccgcac gtcgagaaaa ccgagtacga
     2041 ctcgcagaat gatttcgtcg gcgtcagcct gatcgcttcc tcgtaccacg tgatggcggt
     2101 gcatccctcg ctgccggtgc gctcggtcaa ggagctgata gcgttcgcga aggcacgccc
     2161 gggcgagctc tattactcct cgggcaacat ctggacccca acccacctcg tgcccgagct
     2221 gtttaacgcg gctacgggca cccgtctggt tcccattcac tacaagggct cgggaccctc
     2281 cgtggtcggg gtgctgacgg gggagaccca ggtaatcatg tcaagcgtga cggccgtgat
     2341 gccccacgtg cgctcgaaac gcctggtggc gctcgctgtc accagccccg cgcgcacgcc
     2401 ggttgcgccg gaggtgccga cgctggtgga gctcggcgta aggggcgtgg aggcgccctc
     2461 atggtacgcg atcgtggcgc ccgcggcgac ggcgaggccg atcgtcgaca agcttcacgg
     2521 cgagcttgcg gcaatgtcca cgcagcccga tttccaggca ctgttccgaa agcaggcgct
     2581 ggaaccgacc tccaagacgc cggaggagtt cgccgtattc ctgagaggcg agtacgacaa
     2641 gtggggcaag atcatcaagt cgctcaggag ccagaagtga gggcgtgatt ggtgacaggg
     2701 gcagcttccg cgcgcagcgc gggtgcccta tcacttgtca cccgtcaccc gtcacctgtc
     2761 acgccttttc ttcaggtttc gagcggcagc cgcaccggct tgccggtctc cacggcgcgc
     2821 tcgatggcga gggtggtttc gaggtttacg cgcgcctgct cgggcgtcgt gtgcgcggtc
     2881 gggttgccgg tgacgaggtg gtcgagccag gcgcgggttt cattgccgag cgagccccag
     2941 aagtccccga gcgcccagtc gccggccgaa ttgctgccga gaaaagccat atggaggctg
     3001 tggcccggca catacgggtg cggcacgcct ttctcggtaa acagcaggtg ctccttgtgg
     3061 tcgtcgtcga tcagcatggt gccctcggtg cccagcagct cgacgcggtc ggattgcccc
     3121 tgggtgggaa actgcgccgg cagcgcgtag ctgatgccga agttcaccac cgcgccgtcg
     3181 gcaaaagtga cgatggccca ggtggcgtcg tgcgcgctgt agccggcttc cttgaaaatg
     3241 ccgtgctgcc cgcgcgccac cacttccacc ggacggttgc cctcgagaaa ccagcacatc
     3301 aggtccacgt agtaggtgag cacgtcgagc accggggtcg cgtgggggtc gcgcttgagg
     3361 atggcgaacg tctgggcacg cgagttgtag acgcgggcga gaccgccgac cacctttccg
     3421 agccggccgt ggatcatctg ctccttggcg cggagaaagc attccttgta gcggcggctg
     3481 tagccgatgc gcagctctcc gcccgtggac ttcagcgtct ccaggatgtc gtcggcgtcc
     3541 ttcaatgaga gcgcgatcgg cttttcgcac aagaccggct tgccgagctc cagcgcacgg
     3601 cgcaccgggg cggagtgcag gctctccggc gtcgatacga acacggcgct gacctccggc
     3661 cgctcgatcg cggcaaagtt gctgccggta tggaagtcgg cgtccgtcac gtccgcgagc
     3721 gctttcgcgc gccggggatc gagatcggca atcgccagga agccgaccgc gggatgccgg
     3781 gccgccagcc gcgcccgcag cgtgccgatg cggccggcgc cgatgacggc gacgcccagt
     3841 tccttttgtt tcccttcccg tttcatccat tcacctcttg ccggccctga gggggaacgg
     3901 cgtgaacgcc gggcgcccag ccggatggcg cgaggtgctt ttcggcgaac gccagtgctt
     3961 cggcctcgat gcgggtggcc agcgtgatgt tccagcgatt gagccagccg agcagcgaga
     4021 tcacggcgac gacctcggcc gctgcgtcct ggccgtagta cttcccgagt tccgcgaagt
     4081 gcgcatccgt cacgcgcggc gggcagctgc cggcggcgct tgcgagttcg agcgcggcgc
     4141 gttcgcccgg cgtaaaaaga gtgctcgatc ggaaatgcgg cagggcttcc actttcccga
     4201 cgggaacccc cgcgatatgg acggcgttct cggcgttgtg agcggcgcaa tacggatcgc
     4261 cggcggcagt gctgacgacg caggcgatca tgaacttgag cgctttcggc acttctccgt
     4321 cgtccatcat tacggcggtg tggaggtcgc gataggcccg gaatacccgt ggcttgcgcg
     4381 ccatggcgag ccagctgttg ggcgggtagc ctttcgtctt cttccagcgt tccatgtgat
     4441 cggcgacctg cgcgaactgc tccgccgacg ccggggggat gcgtgccata tcgcttcctt
     4501 gggaaatgcc gcgccgccgg gcgcggtcag tacacgcggt tcaacggcgc ttcgcccctg
     4561 accaggacgc gctcgacgtt ggcccaggct tcgcggaaga gggtggcgga ggcctggagc
     4621 gtgacgcccg cgacgtgggg cgtgagcagc acgcggtggg cggcctcgcc ttcaagggag
     4681 aacaacgggt tgtcggtatc gggcggctcg accgaataga cctccaccgc ggcgcccccg
     4741 aggcggccgg aactcaggtg ggcggccagc gcggtttcgt tgaccacgcc gccgcgcgag
     4801 gcctgtatca gcaccgcgcc gggtttcatc acggcgagct gccgggcgcc gatcatgctg
     4861 cgggtctgcg gcagcagc
//
LOCUS       DBNGSG010000024         3510 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1874922, whole genome shotgun sequence.
ACCESSION   DBNGSG010000024 DBNGSG010000000
VERSION     DBNGSG010000024.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1874922"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="AC158Q_00530"
     CDS             complement(<1..1017)
                     /locus_tag="AC158Q_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HIR3338282.1"
                     /translation="MEAAHAHHGHGEHAHHPYGIMRWITTTNHKDIGTLYLIFSLTMF
                     FVGGLLALMLRLELFEPGMQFWRPEFFNQLTTMHGLIMVFGAIMPAFVGFANWQIPMM
                     IGAPDMAFARMNNWSFWLLPPAAILLVASFFVPGGATAAGWTIYAPLSTQMGPGMDLA
                     IFALHIMGASSIMGSINIITTILNMRAPGMTLMKMPLFVWTWLITAYLLIAVMPVLAG
                     AVTMLLTDRHFGTNFFNAAGGGDPVMFQHIFWFFGHPEVYIMILPGFGIVSQIVPTFS
                     RKPLFGYSSMVYATASIAILSFIVWAHHMFTVGMPASAQLFFMYATMLIAVPTGVKVF
                     NWVAT"
     gene            complement(1052..2191)
                     /gene="coxB"
                     /locus_tag="AC158Q_00535"
     CDS             complement(1052..2191)
                     /gene="coxB"
                     /locus_tag="AC158Q_00535"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HIR3338283.1"
                     /translation="MISKGLRLFAAAGTGLGAEAALANRYNLQEPQSIIAHQIYDLHS
                     LIMWIILGIFVVVFGAMTYSIVVHRKSVGHKAAQFHEHVGVEIAWTIIPFIILIFMAL
                     PATKTIIAMKDTSSADITIKATGYQWKWGYDYLQEGISFYSSLSTPRDQIDDYERKGT
                     KKNENYLIEVDNPVVVPVGKKIRIITTANDVIHAWWVPALGIKQDAIPGFVRDTWFKA
                     DKPGTYRGQCAELCGKDHGFMPIVVEAVTPEQYASWVSGQKQKIAAAAPDPARQWTLD
                     ELKAQGEKIYSSNCVACHQATGMGVPGTFAALSGSKIVTGPKAAQIDVVLNGVVKNGK
                     PTLMAAFGKQLSDVEIAAVITYTRNNWANKTGDMVTPAEVKAARK"
     gene            complement(2222..2713)
                     /locus_tag="AC158Q_00540"
     CDS             complement(2222..2713)
                     /locus_tag="AC158Q_00540"
                     /inference="COORDINATES: protein motif:HMM:NF021517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HIR3338284.1"
                     /translation="MEFSELAPAAGFTRVARRNNSLSSTGRLLAFGFIFVVSVGIAVA
                     FAIIGAWPILPFAGLEMLVLLLAFRAIERRAADYERIAIDGDRVRVEIREAGQARDHE
                     FNRLWAQVVVSRDGAQLALRSHGREVAIGRHLNDGQRLALARELEHRLRGNWPEAAPH
                     TRT"
     gene            complement(2841..3500)
                     /gene="bioD"
                     /locus_tag="AC158Q_00545"
     CDS             complement(2841..3500)
                     /gene="bioD"
                     /locus_tag="AC158Q_00545"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015602386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HIR3338285.1"
                     /translation="MTGTDTGVGKTLVACGLLRAFAARGMKAAGMKPVACGAIPGAGG
                     LVSDDVEQLIAAGNVAAPREHVNPYCFAPPIAPHIAARQAGATISLDRIEQSFRALAA
                     CAQVVVVEGVGGFRVPLGAGIDTAHLAARLALPVVLVVGVRLGCLNHTLLTVESVAAS
                     GLRLAGWIANHIDPRMDAAEDNVRALEALVAAPLLARIAFDAGSETARIAAQLDTRKL
                     A"
BASE COUNT          640 a         1127 c         1110 g          633 t
ORIGIN      
        1 ggtggcgacc cagttgaaca cctttacccc cgtgggaacc gcgatgagca tcgtggcgta
       61 catgaagaaa agctgggccg aggccggcat gccgacggtg aacatgtggt gcgcccagac
      121 gatgaacgaa aggatcgcga tcgaggccgt cgcgtagacc atcgaggagt agccgaagag
      181 gggcttcctc gagaacgtcg gcacgatctg cgacacgatg ccgaagcccg gcaggatcat
      241 gatgtagacc tcgggatggc cgaagaacca gaagatgtgc tggaacatca ccgggtcgcc
      301 gccgcccgcg gcgttgaaga aattggtgcc gaaatggcgg tcggtgagca gcatcgtgac
      361 cgcgcccgcg agcaccggca tcaccgcgat cagcaggtag gcggtgatga gccaggtcca
      421 cacgaacagc ggcatcttca tcagcgtcat ccccggagcg cgcatgttga ggatggtggt
      481 gatgatgttg atcgagccca tgatcgagga cgcgcccatg atgtgcagcg cgaagatcgc
      541 gaggtccatc cccggcccca tctgggtcga gagcggcgcg tagatggtcc agcccgccgc
      601 ggtcgcgccc cccggcacga agaacgaggc gaccagcagg atcgccgccg gcggcagcag
      661 ccagaacgac cagttgttca tgcgcgcgaa cgccatgtcg ggcgcgccga tcatcatcgg
      721 gatctgccag ttggcgaagc cgacgaaggc cggcatgatc gcgccgaaca ccatgatgag
      781 cccgtgcatg gtggtgagct ggttgaagaa ctctggacgc cagaactgca tgccgggctc
      841 gaacagctcg aggcgcagca tgagcgcgag cagcccgccg acgaagaaca tggtcaggct
      901 gaagatcaag tacagcgtgc cgatgtcctt gtgattggtc gtggtgatcc agcgcatgat
      961 cccgtaggga tggtgcgcgt gctcgccgtg gccgtggtgt gcgtgtgcgg cttccatagc
     1021 gttctccaga taacccttcg tagcccgtcg tttacttccg cgccgccttg acttcggccg
     1081 gcgtgaccat gtccccggtc ttgttggccc agttgttgcg cgtgtaggtg atcactgccg
     1141 cgatctcgac gtcggacagc tgcttgccga atgcggccat cagggtcggc ttgccgttct
     1201 tcaccacgcc gttcagcacg acatcgatct gcgccgcttt cggacctgtc acgatcttgg
     1261 agcccgacag ggcggcgaat gtgcccggca cgcccatgcc ggtcgcctgg tggcaagcga
     1321 cgcaattgct tgaatagatt ttctcgccct gcgctttcag ctcgtcgagc gtccactgcc
     1381 tggcggggtc cggcgccgcg gcggctattt tctgtttctg cccgctcacc cacgaggcgt
     1441 actgttcggg cgtcactgcc tcgaccacga tcggcatgaa gccgtgatcc ttgccgcaca
     1501 gttccgcgca ctgcccgcgg taagtgccgg gcttgtcggc cttgaaccag gtatcgcgca
     1561 cgaatccggg aatggcgtcc tgcttgatcc ccagcgccgg cacccaccag gcgtgaatga
     1621 cgtcgttggc cgtggtgatg atccggattt tcttgccgac cggcaccacc accgggttgt
     1681 ccacctcgat gaggtagttc tcgttcttct tcgtgccttt tctctcgtaa tcatcgatct
     1741 gatcgcgcgg cgtggaaaga ctgctgtaga agctgatgcc ttcctgcagg taatcatagc
     1801 cccacttcca ctggtaaccc gtcgccttga tcgtgatgtc ggcgctggag gtgtccttca
     1861 tcgcgatgat ggtcttggtc gcgggcaggg ccatgaagat gaggatgatg aacggaatga
     1921 tggtccaggc gatctcgacg ccgacatgct cgtgaaattg cgccgccttg tgcccgaccg
     1981 acttgcggtg cacgacgatg gagtaggtca tcgcgccaaa cacgaccacg aagatgccga
     2041 ggatgatcca catgatcagc gaatgcagat cgtagatctg gtgcgcgatg atggactgcg
     2101 gttcctgcag gttgtagcgg ttggccagcg ccgcttccgc gccaaggccg gtccccgcgg
     2161 ccgcaaacaa tcgtaacccc ttgctgatca tgcttccctc gccaatgagc ctgatgcgtt
     2221 gttatgtccg cgtatgcggt gcggcttccg gccagttgcc gcgcagccgg tgctccagct
     2281 cgcgcgccag cgcgagccgc tggccgtcat ttagatgccg gccgatcgcc acttcgcggc
     2341 cgtgggaccg cagcgcaagc tgcgctccgt cgcggctcac caccacctgc gcccagagcc
     2401 ggttgaactc gtgatcccgc gcctgccccg cttcgcggat ttccactctc accctgtcac
     2461 cgtctatcgc gatgcgctcg tagtctgcgg cacgccgctc gatggcgcgg aatgccagca
     2521 acaggaccag catttcgagt ccggcaaacg gcaatatcgg ccatgcgccg ataatcgcaa
     2581 acgcaaccgc gatgccgacc gaaacgacga agatgaagcc aaatgcgaga agcctgccgg
     2641 tggaactgag cgagttgttg cgcctggcga cccgtgtaaa cccggccgcg ggtgcaagtt
     2701 cgctgaattc catacttgcg gtcatttttt ccagcaataa cgtccgcgtc gagcgtgtga
     2761 aatgtatcac aggcctctct gcgaggcaac cgaatggcgg tgcaaaacct cagcctgcgc
     2821 aacgagttgc gctgcgcgag tcacgccagt ttgcgggtat ccagctgcgc ggcaattctc
     2881 gcggtttccg agccggcgtc aaacgcgatc cgtgcgagca agggagcggc gacgagcgcc
     2941 tcgagcgcgc ggacattgtc ttccgccgcg tccatccgcg gatcaatgtg attggcgatc
     3001 cagcccgcga gcctgagtcc gcttgcggcc accgattcca ccgtcagcag cgtgtggttg
     3061 agacaaccga gccgcacacc gaccaccagc accaccggca gcgcgagccg cgcggcgagg
     3121 tgcgcggtgt cgattcccgc gccgagcggc acgcgaaaac cgcccacgcc ctcgaccaca
     3181 accacctgcg cgcacgcggc gagcgcccgg aagctttgct cgatccgatc gagcgagatc
     3241 gtggcgcccg cctgccgcgc cgcgatgtgc ggcgcaatcg gcggcgcaaa acagtacgga
     3301 ttgacgtgtt cccgcggggc ggcaacattg cccgccgcga tcaattgttc gacgtcgtcg
     3361 ctgaccagcc cgcccgcccc gggaatggcg ccgcacgcca cgggcttcat gccggccgct
     3421 ttcatgccgc gcgccgcgaa cgcccgcagc aaaccgcatg cgaccagggt cttgccgacg
     3481 ccggtgtcgg tgccggtcac gaagaagcag
//
LOCUS       DBNGSG010000025         3991 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_7959, whole genome shotgun sequence.
ACCESSION   DBNGSG010000025 DBNGSG010000000
VERSION     DBNGSG010000025.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_7959"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..953
                     /locus_tag="AC158Q_00550"
     CDS             <1..953
                     /locus_tag="AC158Q_00550"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012338582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HIR3338286.1"
                     /translation="QTSLDAGDLDDRRLKRVFDQVSRDVPVGRGQHKEARALTGAAKP
                     FKRDLGRILDKHPQLEKRFPRTQNWVCQMGTLGGGNHFIEVCLDESGQVWVMLHSGSR
                     GIGNAIGTYFIELARRDAERRELRLPDRDLAYFEEGAVHFGDYVEAVGWAQDYAAANR
                     HAMVELVLEALRRHLPAFEVTGEVVNCHHNYVERERHYGADVWVTRKGAIRARAGDLG
                     IIPGSMGARSYIVRGRGSEESFQSCAHGAGRRMSRTQAQKTFTPRDVAEQTLGVVCRK
                     DRGVVDEIPGAYKDIDEVMANQGDLVETVHTLKQVVCVKG"
     gene            1100..1792
                     /locus_tag="AC158Q_00555"
     CDS             1100..1792
                     /locus_tag="AC158Q_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HIR3338287.1"
                     /translation="MEREYFVILEHLLVALAAGGLIGFERSYHGRPAGFRTHTLVCVA
                     SSALMLVTMYQSQWYPEAFAGRVALDPTRMAQGIMTGIGFLGAGVIFKEGLTVRGLTT
                     AASIWITAAIGILAGIGFYFPLIVTTLLTLGTLSVFRWIEAKVPGEFYAHLLVRCPRD
                     GGMNEEELRRFLLDHGFSVANMNYAGRMEPPSFEYQMVIKSKDESRGRGLAGALGKSG
                     KVLEFRMSPTGD"
     gene            1898..2971
                     /locus_tag="AC158Q_00560"
     CDS             1898..2971
                     /locus_tag="AC158Q_00560"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3338288.1"
                     /translation="MACLTAISGLGAKGPACFLLEAGRARLLLDLGYGPAPGQWPDVS
                     GVGRVDALLLSHGHRDHAGALKFLPDIGGPPLHATAAVLARLGRDGKSLPLQGEADVC
                     GIRVRTGRNGHAPGGVWLHFDIEGGFLYTGDYGVESPIYAYDAPPPAATVVLDASYGD
                     YSDSLMDCAARLAPVFERGAALVPVPPDGRGPELAYHVAAAHGILPCLSADLRASIER
                     LAGIEGGSLRPGVADGLKRIAREAPAIAGPRGIILAGRADAAGGEAADLAAQWERERS
                     PEIIFSGYFPPGSPAQRMVDSGRASAIRWNAHPRLADNTALARSVRAKAIVPAFGDAS
                     RYRDAWRKAFAPARVVIEREIEL"
     gene            3165..3728
                     /locus_tag="AC158Q_00565"
     CDS             3165..3728
                     /locus_tag="AC158Q_00565"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR3338289.1"
                     /translation="MSNLPPIPLFARPFYFLRHGETESNRLKTIAGSLDVALNEAGIV
                     QARAAIERVRPLGVTHLASSNLRRARETAAIIARALALPHVVVPELAERSWGELEGRS
                     QVLRVRGAPPPPGAETAEQFITRTQGALAQVKADGVPLIVAHSGTHRVLCRLLGLAET
                     VDAIANCRPVRFAPPALPGAAWTVELL"
BASE COUNT          673 a         1258 c         1349 g          711 t
ORIGIN      
        1 cgcaaacctc gctcgatgcc ggcgatctcg acgaccggag gttgaagcgg gtgttcgacc
       61 aggtctcgcg cgacgtgccg gtggggcgcg ggcagcacaa ggaggcgcgc gcactgaccg
      121 gcgccgcgaa gccgttcaag cgcgaccttg ggcgcatcct ggacaagcac ccgcagctcg
      181 agaagcgctt tccgcgcacg cagaactggg tgtgccagat gggcacgctg ggcggcggca
      241 accatttcat cgaggtgtgt ctcgacgagt cgggccaggt ctgggtgatg ctgcactcgg
      301 gcagccgcgg catcggcaac gccatcggca cctatttcat cgagctggcg cggcgcgacg
      361 ccgagcgccg cgagctgcgg ctgccagacc gcgatctggc ctatttcgag gaaggcgcgg
      421 tgcacttcgg cgattacgtc gaggcggtcg gctgggcgca ggactacgcg gccgccaacc
      481 ggcacgccat ggtagagctc gtgctcgaag ccctgcgccg ccacctgccc gcattcgagg
      541 tcacggggga ggtggtgaat tgccatcaca actacgtcga gcgcgagcgc cattacggcg
      601 ccgacgtgtg ggtgacgcgc aagggcgcca tccgggcacg cgccggcgat ctcggcatca
      661 ttcccggcag catgggtgcg cgttcctaca tcgtccgcgg gcgcggctcg gaggagagct
      721 tccagtcctg cgcccacggc gccggccggc gcatgagccg cacgcaggcg cagaaaacgt
      781 tcacgccgcg cgacgttgcc gagcagacgc taggcgtggt gtgccgcaag gatcgcggtg
      841 tcgtcgacga aatccccggc gcctacaagg acatcgacga ggtcatggcg aaccagggcg
      901 atcttgtcga gacggtgcac acgctgaagc aggtggtttg cgtcaaggga tgaaaaagca
      961 ccaggcgctt tttcatccac agtactgatt acgcgtttcc tgatccgttc tgtccacggt
     1021 tcgtggccca gcactggatc ctgcgtatgg agattacatt tttgttacac tttccatata
     1081 gtcttccagg acggaacgga tggagcgcga atatttcgtc atactcgaac acctgctggt
     1141 tgcgctcgcc gccggcggat tgatcgggtt cgagcgcagc taccacggcc gccccgccgg
     1201 ctttagaacc cacacgctgg tgtgtgttgc ttcgagcgcg ctgatgctgg tgacgatgta
     1261 ccagagtcag tggtacccgg aagcctttgc cggacgggtt gccctcgacc cgacgcgcat
     1321 ggcgcagggc atcatgaccg gcatcggctt tctcggtgct ggggtgattt tcaaggaagg
     1381 gctgactgtc cgggggctaa ccactgccgc ttcgatctgg atcacggccg cgatcggcat
     1441 cctcgccggc atcgggttct attttccgct gatcgtcacg accctgctga ccctcggcac
     1501 gctctcggtg tttcgctgga tcgaggccaa ggtgccgggc gaattctacg cgcatctgct
     1561 ggttcgctgc ccgcgggatg gggggatgaa cgaggaggaa ttgcggcgtt ttctgctcga
     1621 ccatggcttt tcggttgcca acatgaatta cgccggccgc atggagccgc ccagcttcga
     1681 gtaccagatg gtgatcaagt cgaaggacga gtcgcgcggg cgggggctgg ccggggcgct
     1741 gggcaagtcc ggcaaggtcc tggaattccg catgtcgccc accggcgatt gaaatccgtg
     1801 attcgtgagt cgtgatgggt gattcgacag gaatgcaggt ccacgcccaa acgcatcacg
     1861 aatcacccat cacgattcac gcctttccgc acctccaatg gcgtgcctga ccgcgatttc
     1921 ggggctggga gcgaaaggcc ccgcctgttt cctgctcgag gccgggcgtg cgcgcctcct
     1981 gctcgacctt ggttacggac ccgcgcccgg ccagtggccg gacgtgagtg gggtggggcg
     2041 ggtggacgca ctgctcctgt cgcacgggca ccgtgatcac gcgggcgccc tgaaattcct
     2101 gcccgacatc ggcggtcccc cgctgcacgc gacggcggcc gtgctggccc gccttggccg
     2161 cgacggcaag tcgctgccgc tgcagggcga ggcggatgtc tgcggcatca gggtgcgcac
     2221 cggacgcaat ggccatgcgc cgggcggcgt ctggctgcac ttcgatatcg agggcggctt
     2281 cctctacacc ggcgactacg gcgtggaatc cccgatctac gcgtacgacg cgccgccgcc
     2341 cgccgccacc gtcgtcctgg atgcgtctta cggagactat tcggactcgc taatggattg
     2401 cgccgcgcgc ctggcgccgg tcttcgaacg cggagcggcg ctggtgccgg tgccgcccga
     2461 cggccgtggc ccggagctgg cgtaccatgt cgcggccgcg cacggcatcc tgccgtgcct
     2521 cagcgccgat ttgcgcgcgt cgatcgagcg cctggcaggc atcgagggcg ggagcttgcg
     2581 ccccggcgtg gcggacggac tcaagcgcat cgcgcgcgag gcgcccgcga tcgcgggacc
     2641 gcggggcatc attctggccg ggcgcgcgga cgcagccggt ggcgaggcgg cggacctcgc
     2701 ggcccaatgg gagcgcgagc gcagccccga gatcatattc agcggctact tcccgcccgg
     2761 ctccccggcc cagcgcatgg tggactccgg acgggcgagc gccatacgct ggaacgcgca
     2821 cccgcggctt gccgacaaca ccgctctcgc gcgctcggtc agggcaaaag ccatcgtgcc
     2881 ggcattcggc gacgcaagcc gttaccggga cgcgtggcgc aaggcgttcg cgccggcgcg
     2941 cgtggtcatc gagcgcgaga ttgaattgtg aaaagccggg aacagacagg attcactcag
     3001 aggaccgaaa acccgttaac cccctaatag acaggattta catgattaac atgattagaa
     3061 cgaacactgg ttcaaaatca ggttattgtt tttgattgaa tcttgtaaat cctgttaatc
     3121 ctgtctattg atgattttga cgggtttttg aatggtcctg tccattgagt aatttaccgc
     3181 ccattccgct cttcgcgcgg ccgttttatt tcctgcggca cggcgagacg gaaagcaacc
     3241 ggctgaagac gatcgcgggc tcgctcgatg tcgcgctcaa cgaagccggg atcgtccagg
     3301 cgcgtgctgc gatcgagcgg gtacggccgc tgggcgtgac ccacctcgct tcgagcaacc
     3361 tccggcgcgc gcgggagacc gcagcgatca tcgccagggc gctggcgctg ccgcacgtgg
     3421 tggtgccgga gctggcggag cgaagctggg gagagctcga aggcaggtcg caggtcctgc
     3481 gtgtccgcgg cgcgccgccg ccgccgggtg cggagaccgc cgagcagttc atcacccgca
     3541 cgcagggcgc gctggcgcag gtgaaagccg atggcgtgcc gctgatcgtc gcccactccg
     3601 gcacgcaccg cgtgctgtgc cgcctgctcg ggctggcgga aacggttgac gcaatcgcga
     3661 actgccgtcc ggtgcgcttc gcgccgcccg cgctgccggg cgcggcgtgg accgtggaac
     3721 ttctctaacg ccgggacaca aaggatacgg aggatagtga taagaggctt cggtttcctc
     3781 tttgtccttc ttgtcctctg tgtctaagag tccctaacat aatgaaacgc gcgctgcgct
     3841 gtcacgattt tggctcaggg cgcgaagcga accgcagcca atatgtcgat attggcaagg
     3901 cgagcgacaa agccatgggc caaaactcgt gccagcccct gagttgtcgt tggatattca
     3961 aacgcctgcg acggggttgc ggccaaaatc g
//
LOCUS       DBNGSG010000026         4545 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1026901, whole genome shotgun sequence.
ACCESSION   DBNGSG010000026 DBNGSG010000000
VERSION     DBNGSG010000026.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4545)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4545)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4545
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1026901"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..715
                     /gene="rplA"
                     /locus_tag="AC158Q_00570"
     CDS             26..715
                     /gene="rplA"
                     /locus_tag="AC158Q_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011901891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HIR3338290.1"
                     /translation="MASKRYDAIRAKVDRAKAYPVQEALKLVKDGATAKFNESVDVSI
                     NLGIDASKSDQTVRGAVVLPQGTGKSVRIAVFAQGDKAAQAKSAGADLVGFEDLANEI
                     KGGKMDFDVVIATPDAMRVVGQLGQILGPRGLMPNPKVGTVTQDVAGAIKNAKAGQIQ
                     YRADKTGIVQCTIGRASFTVDALSENLKALVDAVNKSRPTGVKGIYLKKVSVSSTMGA
                     GVRVDQASLQP"
     gene            1082..1612
                     /gene="rplJ"
                     /locus_tag="AC158Q_00575"
     CDS             1082..1612
                     /gene="rplJ"
                     /locus_tag="AC158Q_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005431581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HIR3338291.1"
                     /translation="MSLNLEQKQAVVAEVSEQIAKAQAVILAEYRSIPVKDMTELRKK
                     ARGSGVYLRVLKNTLARRAVADTPFKGLTEKMVGPLAYGISSDPVAVAKVLQEFAKEN
                     EKFVIRAGAMPNVMMSAREVADLARMPSRQELLAKLVATMQAPIVKFVRTLNEVPGKF
                     VRTLAAVRDQKEKQAA"
     gene            1681..2061
                     /gene="rplL"
                     /locus_tag="AC158Q_00580"
     CDS             1681..2061
                     /gene="rplL"
                     /locus_tag="AC158Q_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HIR3338292.1"
                     /translation="MAVAKAEILDAIANMTVLELSQLIKDMEDKFGVSAAAAVAVAAP
                     AAGGGAAAVAEEKTEFTVNLTTTGANKVNVIKVVRAVTGLGLKEAKDLVDGAPKAVKE
                     GISKADAEAILKQLVEAGATGEIK"
     gene            2214..>4545
                     /gene="rpoB"
                     /locus_tag="AC158Q_00585"
     CDS             2214..>4545
                     /gene="rpoB"
                     /locus_tag="AC158Q_00585"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006029840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIR3338293.1"
                     /translation="MAYSFTEKKRIRKSFAKREVVLPVPFLLATQLDSYAGFLQAGVA
                     PDARKTEGLKAAFKSIFPIESHSKNARLEFVSFALGEPPFDVKECQQRGLTYAAPLRA
                     KVRLVIMDKEAPKPTAKEVKEQEVYMGEIPLMTTTGSFVINGTERVIVSQLHRSPGVF
                     FEHDRGKTHSSGKLLFSARIIPYRGSWLDFEYDPKDYLYFRVDRRRKMPVTILLKALG
                     YTPEKILAEFFAFDGFLLGKDGVEFELVPERMRGEIARFDINTKGGKAIVAKDKRITV
                     KHVREMEQAGLHKIAVPDEFLIGRVLAQNVVDKGTGEVLANANDEITEELLAKLKEAG
                     VNKIQTIYTNDLDQGPYISQTLRIDETADQLAAQVAIYRMMRPGEPPTEEAVKTLFHR
                     LFFAEETYDLSRVGRMKFNRRVGRTELTGPAKLSAEDIVAVIKILVDLRNGKGEIDDI
                     DHLGNRRVRSVGELAENQFRAGLVRVERAVRERLSQAESENLMPHDLINAKPVSAAIR
                     EFFGSSQLSQFMDQTNPLSEITHKRRVSALGPGGLTRERAGFEVRDVHPTHYGRVCPI
                     ETPEGPNIGLINSLAIYARTNEYGFLETPYRKVKDSRVSDEIHFLSAIEEGQYMIAQA
                     NAALDKQNRFTDELVSCRNRNEFTLSAPDRIEYMDVAPSQAVSVAASLIPFLEHDDAN
                     RALMGSNMQRQAVPCLRPEKPLVGTGLERTVAVDSGTAVQARRGGIVDYVDASRIVVR
                     VNDEETAPGEVGVDIYNLVKYQRSNQNTNINQRPLVK"
BASE COUNT          943 a         1337 c         1501 g          764 t
ORIGIN      
        1 gggcgtggaa gtggagggtc tgtaaatggc ttccaaacgc tatgacgcaa ttcgcgccaa
       61 ggtggatcgc gccaaggcct acccggtgca ggaagcgctg aaactcgtga aggacggcgc
      121 taccgccaag ttcaacgaat cggtggatgt ttccatcaac ctcgggatcg atgcctcgaa
      181 atccgaccag acggtgcgcg gcgccgtggt gctgccgcag ggcaccggca agagcgtgcg
      241 gatcgcggtg ttcgcccagg gcgacaaggc ggcccaggcg aagagcgccg gcgccgacct
      301 cgttggcttc gaggatctcg ccaacgagat caagggcggc aagatggatt tcgatgtcgt
      361 gatcgcgacg cccgatgcga tgcgggtggt gggacagctg ggccagatcc tgggcccgcg
      421 aggcctcatg ccgaacccca aggtgggcac cgtcacgcag gacgtggcag gcgcgatcaa
      481 gaacgcgaag gcgggccaga tccagtaccg cgcggacaag acggggatcg ttcaatgcac
      541 gatcggccgc gcatcgttca cggtggacgc cctgtccgag aatctgaagg cgctggtgga
      601 cgcggtcaac aagtcacggc ccacgggcgt gaaagggatc tacctcaaga aggtatcggt
      661 ctcgagcacg atgggcgcgg gcgtgcgcgt ggaccaggca agcttgcaac cataggagaa
      721 ggaacgaggg gatattcccc ccgcgccccc ctactactgg gaccgtttca gtttcccgga
      781 aacggcctca atgaaaccag gcacccggac cgcggttcgg ggcgggaaag aactttgggc
      841 cgccagaggc gcaccgctgg cgggttgtca aagaccgtag gaacccgtgt gttttaagtg
      901 agggtttaat ttttagggaa cggatcaggg taccaagggt cgaagctgcc cccgggcggt
      961 cgctcaaccc tcgattctca atcctcattc ccgattgtcc tacgcagacg gtgtgcccga
     1021 aacaggttat gcagttgcct cctggatcac cggtcgccgt gttagtgaaa ggagatagac
     1081 cttgagtcta aatctggagc agaagcaggc ggtagtggcc gaggtgagcg aacagattgc
     1141 caaggcccag gccgtgatac tggccgagta ccgcagcatc ccggtgaagg acatgaccga
     1201 attgcgcaag aaggcgcgcg gctcgggcgt gtacctgcgg gtgctgaaaa acaccctggc
     1261 gcgacgcgcg gtcgccgata cgccgttcaa gggactcacc gagaagatgg tggggcccct
     1321 ggcttacggc atttcgagcg atcccgtggc cgtggccaag gtgctgcagg agtttgccaa
     1381 ggaaaacgag aagttcgtga tcagggcggg cgccatgccc aacgtgatga tgtcggccag
     1441 ggaagtggcg gatctcgcgc gcatgccgag ccgtcaggaa ctgctcgcga aactcgtggc
     1501 gaccatgcag gcgccaatcg tcaaattcgt ccgtaccttg aacgaggtgc cgggcaaatt
     1561 tgtgcggaca ctggcggcgg tcagggacca gaaagagaag caggctgctt agtcaggaat
     1621 tgaagcctgc ttgggaccgg aaattcggat aacgcaatct tagatctagg agcaatgaac
     1681 atggctgttg caaaagcaga aattctcgat gcaatcgcga acatgaccgt gctggagctg
     1741 tcccagttga tcaaggacat ggaagacaag ttcggcgttt ccgcagccgc ggcagttgcg
     1801 gtggcggcgc cggcagccgg cggtggcgcg gcggccgtgg ccgaggagaa gaccgagttc
     1861 acggtgaatc tcaccaccac gggggccaac aaggtcaacg tcatcaaggt ggtgcgcgcc
     1921 gtgaccggcc tcggcctgaa ggaagccaag gacttggtgg acggcgctcc caaggcggtc
     1981 aaggaaggga tttccaaggc tgacgcggaa gctatcctca agcagctcgt cgaagccggc
     2041 gcgaccggcg agatcaagta atcgtgatgt ggagacccgg ccggcgggaa gccgccggcc
     2101 cgtgtcgttc tattttgaac ggtccgcgac tttcccagca cgccgtgcgg cacgcgccgc
     2161 agggcggatt gcggccgtcg ctgtttgcct attgattttt acctggagct gatatggcgt
     2221 actccttcac cgagaagaaa cgcatccgca agagcttcgc caagcgtgaa gtcgttttgc
     2281 cggtgccgtt cctgctggcg acccagctcg actcctatgc gggattcctg caggcggggg
     2341 tcgcgccgga tgcccgcaag accgagggtc tgaaggcggc gttcaagagc atcttcccga
     2401 tcgagagcca ctccaagaat gcgcggctgg agttcgtgag tttcgcgctc ggcgagccgc
     2461 cgttcgacgt caaggaatgc cagcagcgcg ggctgaccta cgcggcgccg ttgcgcgcca
     2521 aggtgcgtct cgtgatcatg gacaaggagg cgcccaagcc cacggccaag gaagtgaagg
     2581 agcaggaagt ctacatgggc gagatcccgc tcatgaccac gaccggctcg ttcgtgatca
     2641 acggcaccga gcgcgtgatc gtgtcccagc tgcatcgctc gccgggggtg ttcttcgagc
     2701 acgaccgcgg caagacccac tcctcgggca agctcctgtt ctcggcacgc atcattcctt
     2761 accgcggttc ctggctcgac ttcgaatacg accccaagga ctatctctat ttccgggtcg
     2821 accgccgccg caagatgccg gtgacgatcc tgctgaaggc cctgggctat acgccggaga
     2881 agatcctcgc ggaattcttc gcattcgacg gtttcctgct cggcaaggac ggcgtggaat
     2941 tcgagcttgt acccgagcgc atgcgcgggg aaatcgcgcg cttcgacatc aacaccaaag
     3001 gcggcaaggc catcgttgcc aaggacaagc gcattacggt caagcacgtg cgcgagatgg
     3061 agcaggccgg ccttcacaag atcgcggtgc cggatgagtt cctgatcggc cgcgtgctcg
     3121 cgcagaacgt cgtggacaag ggcacgggag aagtcctcgc caacgccaat gacgagatca
     3181 ccgaggagct gctcgccaag ctgaaagaag cgggcgtcaa caagatccag accatttaca
     3241 ccaacgatct tgaccaggga ccctacatct cgcagaccct gcgcattgac gagactgcgg
     3301 accagctggc tgcgcaggtc gcgatctacc gcatgatgcg tcccggcgag ccgccgacgg
     3361 aagaggcggt aaagacactg ttccatcgct tgttctttgc cgaggaaacc tacgatctct
     3421 cgagggtcgg gcgcatgaaa ttcaatcggc gcgttgggcg caccgagttg accgggccgg
     3481 ccaagctgtc ggccgaggac atcgtcgccg tcatcaagat cctggtggac ctgcggaatg
     3541 gcaagggcga gatcgacgat atcgaccatc ttggcaaccg gcgcgtgcgc tcggtgggtg
     3601 agctcgcgga gaaccagttc cgcgctggac tggtacgggt ggagcgggcg gtcagggagc
     3661 ggctctcgca ggccgagtcc gagaacctga tgccgcatga cctcatcaat gccaagccgg
     3721 tatcggccgc gatccgagag ttcttcggct cctcgcaact ctcgcagttc atggaccaga
     3781 caaacccgct ttcggaaatc acccacaagc ggcgcgtgtc cgcgctggga cccggggggc
     3841 tgacgcgcga gcgtgcaggg ttcgaggtgc gagacgtgca ccccacgcac tacggccgcg
     3901 tttgcccgat cgaaacgccg gaaggcccga acatcggtct catcaattcg cttgcgatct
     3961 acgcgcgcac caacgagtac ggctttctcg agacgccgta ccgcaaggtg aaggacagcc
     4021 gcgtctccga cgagattcat tttctgtcgg cgatcgagga aggccagtac atgattgcgc
     4081 aggcgaacgc ggcgctcgac aagcagaacc gctttaccga cgagctggtc tcgtgccgca
     4141 accgcaatga attcacgctg tctgcgcccg accgtatcga gtacatggac gtggcgccgt
     4201 cgcaggcggt gtcagtcgcg gcgtcgctca taccattcct cgagcatgac gacgccaacc
     4261 gcgctctaat gggatccaac atgcagcgtc aggcagtgcc gtgcctgcgg ccggagaagc
     4321 cgctggtggg cacgggtctc gagcgcaccg tggcggtgga ctcgggcacc gcggtgcagg
     4381 cgcgccgcgg cggcatcgtg gactatgtgg atgcctcgcg catcgtggtt cgcgtgaacg
     4441 acgaggaaac cgcgccgggc gaggtgggcg tggacatcta caacctggtc aagtatcagc
     4501 gctcgaacca gaacaccaac atcaatcagc ggccgctggt caagg
//
LOCUS       DBNGSG010000027         3694 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1366745, whole genome shotgun sequence.
ACCESSION   DBNGSG010000027 DBNGSG010000000
VERSION     DBNGSG010000027.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1366745"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2020)
                     /locus_tag="AC158Q_00590"
     CDS             complement(<1..2020)
                     /locus_tag="AC158Q_00590"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF020037.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3338294.1"
                     /translation="MSAVAPHVKPALSAAVVRYVLILCVSLVAVALFLLAASSANPAS
                     FAERYPLLLVLNATVALMLTALVAYQFITLRRKLKAGVFGAKLTLRLVLLFALMAVLP
                     GALIYGMSVQFLARSIESWFEVRVDKALEGGINLGRNVLDSALKDLTVKADDMALALA
                     TRPTDEHAAALNTLREQAGVQEATLFSERGKVLVFSGNERVGLMPELPGAIALQRIRA
                     RRPYSAVEALPDKGLYLRALVPVTVPSLTDELRVLQLLQPVPRQLAQDAETVQSGYRE
                     YQELTLSRRGLKRLYGVTLTLTLLLALLSAVALAFVLSDRLSAPLGVLVEGTRAVASG
                     DFSQRAAVASSDEIGMLTQSFNAMTLQLAEARALAERNQAALTQAKAYLESLLANLSA
                     GVLAFDEELNLRAANHSAGTILGIDFAPLLGVAAPRWPETAPGVAELGREITAAFADA
                     SATEWEQQAERLLHGRTQLLLLRGTRLPQGAEGGCVVVFDDITHLAEAQRTAAWAEVA
                     RRLAHEIRNPLTPIQLSAERLQVKLAGKLAEADAEVLARSTQTIVNQVAALKNMVDAF
                     SQYARTPEPSVRELDLNGLTREVLTLYESSLGQSVRLDLAAGLPPVMGDAAQLRQVIH
                     NLLQNAQDALADTRDPRITIASSAEGGMVRLAVTDNGCGFPEQVIQRAF"
     gene            complement(2017..2592)
                     /locus_tag="AC158Q_00595"
     CDS             complement(2017..2592)
                     /locus_tag="AC158Q_00595"
                     /inference="COORDINATES: protein motif:HMM:NF025692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HIR3338295.1"
                     /translation="MLSCRRLSQLIRLAALATLLLGMAAAAPARGDGIDIRKAALVAA
                     EEGYFLEAEFDIALTHTLEEALNKGVPLYFTLEFELIRPRWYWLNEKLANTRQQYRLG
                     YNALTRQYRVGVGALYQNFASLSEALAFLSRVRLRDIVEPGALVKGSSYTAALRMRLD
                     GSQLPRPFQISAVGSREWSVGSDWHRWTVTP"
     gene            complement(2570..>3694)
                     /gene="rsmB"
                     /locus_tag="AC158Q_00600"
     CDS             complement(2570..>3694)
                     /gene="rsmB"
                     /locus_tag="AC158Q_00600"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF008149.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HIR3338296.1"
                     /translation="PLNRDPSYLMFEYACHEGNLALPNALNAGRVRERTRAAPHAVVD
                     HAVRGCKGLGLSSAQGLANAVLRNYLRKQSALDARVQRSETARYSHPQWWIDRLRAQY
                     PDRFAAALDADNLHPPLALRVNRRRSSVSGYLELLERHGIAGESRGEAAVLLEKALPA
                     ARIPGLIEGLVSVQDLAAQLAAPLLDLRDGVRMLDACAAPGGKTGHVLELAEVELTAL
                     DSDGGRLERVHANLTRLGLTARVICGDACDPASWWDGAPFERILADVPCSASGVARRH
                     PDIKWLRRETDIARFANAQGRMLDALWQTLASGGKLLYATCSVFHEENREQVERFLER
                     HRDATRLVLPAVDNDIQLPAGQLLPDGRHDGFFYALLQKA"
BASE COUNT          595 a         1276 c         1246 g          577 t
ORIGIN      
        1 cgaacgcgcg ctggatcacc tgctcgggga aaccgcagcc attgtcggtg accgcgagcc
       61 gcaccatgcc gccttcggcg ctgctcgcga tggtgatgcg cgggtcccgc gtatcggcca
      121 gcgcgtcctg cgcgttctgc agcaggttgt gtatcacctg ccgcagctgc gccgcgtcgc
      181 ccatcaccgg cggcagcccg gcggcgagat cgagccgcac gctctggccg agcgaagatt
      241 cgtagagcgt cagcacctcg cgcgtgagcc cgttgagatc cagctcgcgc acggagggct
      301 cgggcgtgcg cgcatactgg ctgaaggcat ccaccatgtt cttgagagcc gcgacctggt
      361 tgacgatggt ctgggtcgag cgcgcgagca cctcggcgtc ggcttccgcg agcttgccgg
      421 cgagctttac ctgcagccgc tccgcggaga gctggatcgg cgtcaacgga ttgcggatct
      481 catgcgcgag ccgccgcgcg acctcggccc aggccgccgt gcgctgggcc tcggcgaggt
      541 gcgtgatgtc gtcgaacacc acgacgcagc cgccctcggc gccctgcggc aggcgggtgc
      601 cgcgcaacag cagcagctgg gtcctgccgt gcagcaggcg ttcggcctgc tgctcccatt
      661 cggtcgcgct ggcgtccgcg aacgccgccg tgatctcccg acccagctcc gcgacgcccg
      721 gggccgtctc cggccagcgc ggcgccgcaa caccgagcag cggcgcgaaa tcgattccga
      781 gtatcgtgcc cgcgctgtgg ttggcggcgc gcagattcaa ttcctcgtcg aaggccagca
      841 cgccggccga caggttggcg agcaggcttt cgagataggc cttcgcctgc gtgagcgccg
      901 cctgattgcg ctcggccagc gcgcgcgctt ccgccagctg cagcgtcatc gcgttgaagg
      961 actgggtcag catgccgatc tcgtcgctcg acgcgaccgc tgctcgctgg ctgaaatcgc
     1021 cgctcgccac cgcgcgcgtg ccttccacca gcacgccgag gggcgccgac aggcggtcgg
     1081 agagcacgaa cgcgagcgcc accgcggaca gcagcgccag cagcagagtc agcgtcagcg
     1141 tgaccccgta gaggcgcttc aatcctcgcc gcgacagggt gagttcctgg tattcgcgat
     1201 aacccgactg caccgtctcg gcatcctggg cgagctgcct gggcaccggc tgcagcagtt
     1261 gcagcacgcg cagctcatcg gtgaggctcg gcacggtaac cggcaccagg gcgcgcaggt
     1321 agaggccttt gtcgggcagc gcctcgaccg cgctgtaggg cctgcgtgcg cgtatccgct
     1381 gcagcgcgat tgcgccggga agctcgggca tcaatcccac gcgctcgttg ccggagaata
     1441 cgagcacctt gccgcgttcg ctgaacaagg tcgcttcctg cacgcccgcc tgctcacgca
     1501 gcgtattgag cgctgccgcg tgctcgtcgg tcgggcgggt cgcgagcgcg agcgccatgt
     1561 catcggcctt gaccgtgagg tccttcaacg cgctgtcgag cacattgcgc cccaggttga
     1621 tgccgccctc gagcgccttg tccacgcgca cttcgaacca ggactcgatc gagcgcgcca
     1681 ggaactggac cgacatgccg tagatcagcg cgcccggcag caccgccatc aacgcaaaca
     1741 gcaacaccag gcgcagcgtc agcttcgcgc cgaacacgcc ggccttcagc ttgcggcgca
     1801 gcgtaatgaa ctgatacgcg acgagcgcgg tcagcatcag cgccaccgtc gcgttcaaca
     1861 ccagcaggag cgggtagcgt tcggcgaacg acgccgggtt ggcgctcgaa gcggcgagca
     1921 ggaacaacgc gaccgcgacg aggctcacgc acaggatcag cacgtagcgc accacggcgg
     1981 cggacagcgc gggtttgacg tgcggggcga cagcgctcat ggcgttaccg tccagcggtg
     2041 ccagtccgaa cccacgctcc actcgcgcga gccgaccgcg ctgatctgga acggccgcgg
     2101 cagttgcgag ccgtccagcc gcatgcgcag cgctgcggta tagctgctgc ccttgacgag
     2161 cgcgccgggt tccacgatat cgcgcagccg cacccggctg aggaacgcca atgcttcgct
     2221 caacgaggcg aaattctgat agagcgcgcc cacgccgacg cgatactggc gggtcaaggc
     2281 gttgtaaccc agccgatact gctgccgcgt gttagcgagc ttctcgttca gccagtacca
     2341 gcgcgggcgg atcagctcga actcgagcgt gaaataaagc ggtactccct tgttgagcgc
     2401 ttcttccagg gtatgggtga gcgcgatgtc gaattcggcc tcgagaaaat agccttcctc
     2461 ggcggccacc agcgccgcct tccggatgtc gatgccgtcg ccgcgcgcgg gcgccgcggc
     2521 ggccatgccg aggagcagcg tcgcgagcgc ggcgagccgg atgagctggc taagccttct
     2581 gcaggagagc atagaagaag ccatcatgcc gtccgtccgg gagcagttgg cccgctggca
     2641 gttgtatgtc gttgtcgacc gcggggagaa cgaggcgcgt cgcgtcccgg tggcgctcca
     2701 gaaaccgctc aacctgttcg cggttttcct cgtgaaacac ggaacaggtt gcgtacagca
     2761 atttaccacc actggcgagt gtctgccaca gcgcgtcgag catgcggccc tgcgcgttcg
     2821 caaagcgcgc aatgtcggtt tcccgccgca gccatttgat gtcgggatgc cgccgcgcga
     2881 cgcccgatgc gctgcaggga acgtcggcga gaatgcgctc gaacggcgct ccgtcccacc
     2941 aggacgccgg atcgcacgcg tcgccgcaga taacgcgcgc cgtcaacccc agtcgcgtca
     3001 ggttcgcgtg cacccgttca agccggccgc catcgctgtc cagggcggta agttccactt
     3061 cggccagctc cagcacgtgg ccggtcttgc caccgggcgc ggcgcatgcg tcaagcatgc
     3121 gcacgccgtc gcgcaggtcg agcagcggtg cggcgagctg cgccgccagg tcctgcaccg
     3181 acacgagccc ttcgatgagc ccgggtatgc gcgctgccgg cagcgctttc tccagcagca
     3241 ccgcggcttc gccgcgggat tcgcccgcga tgccgtggcg ctcgagcagt tccaggtatc
     3301 cggaaacgct cgagcggcgg cggttcacgc gcagcgccag cggtggatgc aggttgtccg
     3361 cgtccagcgc ggcggcgaaa cgatccggat actgggcgcg cagcctgtcg atccaccatt
     3421 gcggatggga atagcgcgcg gtttcgctgc gctgcacgcg ggcatccagc gccgattgct
     3481 tgcgaagata gttgcgcaat accgcgtttg cgagcccctg cgcggagctc aggccgaggc
     3541 ctttgcaccc gcgcacggcg tgatccacta cggcatgcgg cgcggcgcgc gtgcgctcgc
     3601 gcacgcggcc cgcgttcaac gcgttcggca gcgcgagatt tccttcgtgg caggcgtact
     3661 cgaacatcaa gtagctcgga tcgcgattca gcgg
//
LOCUS       DBNGSG010000028         5760 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1366822, whole genome shotgun sequence.
ACCESSION   DBNGSG010000028 DBNGSG010000000
VERSION     DBNGSG010000028.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5760)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5760)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5760
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1366822"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..156
                     /locus_tag="AC158Q_00605"
     CDS             <1..156
                     /locus_tag="AC158Q_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HIR3338297.1"
                     /translation="VLDAQWSQAVARNPFLGLRVKGAKAGDKISVTWTDNKGDKRTDE
                     VAVSAAS"
     gene            204..1016
                     /gene="soxA"
                     /locus_tag="AC158Q_00610"
     CDS             204..1016
                     /gene="soxA"
                     /locus_tag="AC158Q_00610"
                     /inference="COORDINATES: protein motif:HMM:TIGR04484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HIR3338298.1"
                     /translation="MMPRMRSGLAALAVACLGIAGVALAQSGDPLGVGRKMLVEDNPG
                     DLWIDKGKTFYYEKRGPRNVSLEQCDFGMGPGKLEGAAARLPRYFPDTDKVQDLETRL
                     ITCMVRLQGFDEADVVRRAISPGGSNGSDIEALALFITSRSNGMKVNVTLSHQKEYDT
                     YKAGEYLFFRRSGQTDFGCVQCHGEANKRIRLQDLVHMTSKAGAQEVASTWPAYRGAH
                     FVVRTMQWRLTDCFWQMRLPDMKYGSEMAIALTLYLNHQGNGAVIRVPGFKR"
     gene            1031..1666
                     /gene="soxX"
                     /locus_tag="AC158Q_00615"
     CDS             1031..1666
                     /gene="soxX"
                     /locus_tag="AC158Q_00615"
                     /inference="COORDINATES: protein motif:HMM:TIGR04485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HIR3338299.1"
                     /translation="MNNKGLKWVALAAGALIAAGCATFPNPENTRQISEGMVKSGFTA
                     SPAHAKRLEQDRSQQICSKIAGAPLTQAEAAEVVKLARASIQYPASGKLVGDWKVGNK
                     LAHDGAGDRILRGKLEKRKENGGLCQNCHALAPGEINVGNVGPSLTGYGAQRGNSEAV
                     ARLTYERIYNGWAYAPCSNMPRLGANRHLTPEQITHLVAYLLDPASPINQR"
     gene            1755..3476
                     /gene="soxB"
                     /locus_tag="AC158Q_00620"
     CDS             1755..3476
                     /gene="soxB"
                     /locus_tag="AC158Q_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfohydrolase SoxB"
                     /protein_id="HIR3338300.1"
                     /translation="MSLTRREFLQMLAVAASYGAPLDGNRAFAAAGAPGLYELPKSGG
                     NVTLLHFTDCHAQLLPAYFREPDVNIGVHSARGKPPHLVGEALLKHFKIAPKSPLAHA
                     YTHIDFENAARRYGKAGGFAHLCALVKQVRAQRRGALLLDGGDSWQGSATSLWTRGQD
                     MIDASKLLGVDLMTGHWEFTYGAARVREVLKKDFSGGIEFLAQNVRTLDFGDPVFKSH
                     VIREVNGVPVAIIGQAFPYTPIANPRHFVPEWTFGIQEESLQKVVEETRKKGAQAVVL
                     LSHNGMDVDLKLASRVAGIDVILGGHTHDAVPDPVAVSNRGGTTLVTNAGSNGKFLAV
                     LDLDVRGGHVKGHQYRLLPVFSNLIKPDAEMAQLIRRARAPHEKKLAERLAVTEGLLY
                     RRGNFTGTFDQLILDALMEAKGAEIAFSPGFRWGTALLPGQDITLEHVMDQTAITYPQ
                     VTVNELTGEAIKNLLEDVCDNLFNPDPYYQQGGDMVRVGGMRYRCAPLQKIGARISDM
                     TLKGQPVEAGKRYKVASWAPVAEGVAGEPVWDVVAGYLRAKKVVRPLQPGRPALSGVQ
                     GNPGLSS"
     gene            3476..4462
                     /gene="galE"
                     /locus_tag="AC158Q_00625"
     CDS             3476..4462
                     /gene="galE"
                     /locus_tag="AC158Q_00625"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006408420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HIR3338301.1"
                     /translation="MTILVTGGAGYIGSHMVKLLRKRGRRVVVLDNLATGHRDAVKDA
                     EFIEGDVGDTGKVAALLAGHSVKAVIHFAAYSLVAESVSNPKKYYANNVDATLRMLGA
                     MREARVGRIVFSSTAAVYGEPQASPIEESHPREPINPYGECKLVIERTLDDLHAAHGL
                     RSASLRYFNASGADPDGSLGERHDPETHLIPLVLQAASGRRPAVAVYGTDYPTRDGTC
                     VRDYVHVSDLCDAHLLALEWLEGGGGREAFNLGSESGATVMEVIETARHETGAPIDAR
                     IEGRRPGDPAVLVASCEQARRVLGWSPKRSDLATIVQDAWRWERGRTANHAA"
     gene            4486..5475
                     /locus_tag="AC158Q_00630"
     CDS             4486..5475
                     /locus_tag="AC158Q_00630"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016497.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HIR3338302.1"
                     /translation="MNRSRVAAFSAMLLAACATLSGPDYRNPVLDADFPDPAVLRAPD
                     GWFYAYATQGSVGERMLNVQVARSRDLARWEHLGDALPGKPRWAARKQAFWAPHVLYD
                     RQLNRYYMYYAAEPDDSTGKCLGVATAPDPRGPFADSGAPLICGEGLEHIDPMAFDDP
                     RTGTRLLLWGSGGMPIRARELATDRLRFLPGSASVDLVAPDADRPYRALIEGAWMTYR
                     DGWYYLYFSGDRCCTREPRYAVMVARARRPLGPFEHYPGGRGGSGAILEADERWLAPG
                     HNSIASDAAGNDWIVYHAYHARRFPAGDRRRVMLIGRILYRDGWPTVSGIPSS"
     gene            5597..>5760
                     /locus_tag="AC158Q_00635"
     CDS             5597..>5760
                     /locus_tag="AC158Q_00635"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3338303.1"
                     /translation="MLGGAGFIGAHLAEVLLQAGHHVRIFDRPRVDGLALLEDRAGPE
                     IVTGDFLNPE"
BASE COUNT         1034 a         1883 c         1923 g          920 t
ORIGIN      
        1 gtcctcgacg cccagtggag ccaggcggtc gctcgcaatc ccttcctggg gctgcgcgtc
       61 aagggcgcga aggccggaga caagatcagc gtgacctgga cggataacaa gggcgacaag
      121 cgcacggacg aagtggcagt gtccgcggcg tcctgacggc cgccgggcac ggcccggcga
      181 aaataaaaca ggaggagaaa gacatgatgc cgagaatgcg tagcggcctt gccgcgcttg
      241 ccgtcgcttg ccttgggata gcgggggtgg cgctggcgca gtcgggcgac ccgctcgggg
      301 tcgggcgcaa gatgctggtc gaggacaacc cgggcgatct gtggatagac aagggcaaga
      361 cgttctatta cgagaagcgc ggccccagga acgtctcgct ggaacaatgc gacttcggca
      421 tggggcccgg gaagctcgaa ggcgccgccg cccggttgcc gcgctacttc cccgacacgg
      481 acaaagtcca ggacctggag acgaggctga tcacctgcat ggtgcgtttg caggggttcg
      541 atgaagccga tgtcgtcagg agagcgatca gcccgggcgg cagcaacggc tcggatatcg
      601 aggccctggc gctgttcatc acctcccggt cgaacgggat gaaagtgaac gttacgttga
      661 gccaccagaa ggagtacgac acgtacaagg cgggcgagta cctgttcttc cgccgcagcg
      721 gccagacgga cttcgggtgc gtgcaatgcc acggcgaagc caacaagcgc atccgcctgc
      781 aggacctggt ccatatgacc agcaaggcgg gggcgcagga agtcgcgtcg acctggcccg
      841 cttaccgcgg cgcgcatttc gtcgtccgga ccatgcagtg gcggctcacg gactgcttct
      901 ggcagatgcg attgcccgac atgaagtacg ggtcggaaat ggcgattgct ctgaccctgt
      961 atctcaacca tcagggcaac ggggcggtca tccgcgtgcc cggattcaag cggtgaggaa
     1021 ggggaaaacc atgaacaaca aagggctgaa atgggtcgcg ctcgccgccg gcgccttgat
     1081 cgcggcgggc tgcgcaacct tccccaatcc ggagaacacg cgccagattt cggaaggcat
     1141 ggtgaagagc ggcttcaccg ccagtccggc gcacgccaag cgcctcgagc aggaccggtc
     1201 gcagcagatc tgcagcaaga tcgcgggcgc gccgttaaca caggcggaag ccgcggaagt
     1261 ggtaaagctt gcgcgcgcct ccatccagta cccggcgtcc gggaagcttg tcggagactg
     1321 gaaggtcggc aacaagctgg cgcatgatgg cgcgggcgac aggatcctcc gcgggaaact
     1381 cgagaagcgc aaggaaaacg gggggctgtg ccagaactgt cacgccctcg cccccggcga
     1441 gatcaacgtc ggcaacgtgg ggccgagcct gaccggctac ggcgcgcaac gcggcaattc
     1501 ggaagccgtc gccaggctca cctacgagag gatctacaac ggctgggcct atgccccctg
     1561 ctccaacatg ccgcgcctgg gcgccaaccg gcacctgacg ccggagcaga tcacccatct
     1621 cgtggcctac ctgctcgatc cggcctcgcc gattaaccag cgataagacg ggcgcgttcg
     1681 cgccgtctta tctgcgcgcg ttcgagcgga cgatagattc accgctcaac gcgcgcacct
     1741 gatgccagac tggaatgtct cttacacggc gcgaattcct gcaaatgctg gcggtggcgg
     1801 catcttacgg cgccccgctc gatggcaatc gggcatttgc cgcggccggc gcccctggcc
     1861 tctacgagct gccgaaatcc ggcggcaacg tgaccctgct gcatttcacc gactgccatg
     1921 cccagctgct ccccgcctat ttccgcgaac ctgacgtcaa tatcggcgtg cactccgctc
     1981 gcggcaagcc gccccacctc gtcggcgaag ccctgctcaa gcatttcaag attgcgccga
     2041 agtcgccgct ggcccatgcc tatacgcaca tcgacttcga gaatgcggcg cgccgttacg
     2101 gaaaagcggg cgggttcgcc cacctgtgcg ccctggtgaa gcaggtacgc gcccagcggc
     2161 gcggcgcgct gcttctcgac ggcggcgatt cctggcaggg ttcggcgacc tcgctgtgga
     2221 cgcgaggcca ggacatgatc gatgcctcga agctgctcgg cgtcgacctc atgaccgggc
     2281 actgggaatt cacctacggt gcggcgcggg tccgggaagt gctcaaaaag gacttctcgg
     2341 gaggaatcga gttcctcgcg cagaacgtga ggaccctgga tttcggcgac ccggtgttca
     2401 agtcccatgt gatccgcgag gtaaatggcg tgcccgtcgc catcatcggc caggcgtttc
     2461 cctacacgcc gatcgccaac ccgcgccatt tcgtgccgga gtggacgttc ggtatccagg
     2521 aagaatccct gcagaaggtg gtggaggaaa cgcgaaaaaa aggtgcgcag gcggtcgtgc
     2581 tgctctcgca caacggcatg gatgtcgacc tgaagctcgc ctcccgcgtc gccggcatcg
     2641 acgtcatcct gggcgggcac acccacgacg cggtgcccga cccggttgcg gtgagtaacc
     2701 gcggcggcac taccctcgtc accaacgccg gttccaatgg caagtttctc gccgtgctgg
     2761 acctcgacgt gcggggcggg cacgtgaaag gccatcagta ccgcctgctc ccggtatttt
     2821 ccaacctcat caagccggat gcggaaatgg cgcagctgat ccggcgcgcg cgggcgccgc
     2881 acgagaaaaa gctcgcggag cggcttgccg tgaccgaggg attgctctat cgccgcggaa
     2941 atttcaccgg caccttcgac cagctgatcc tggatgccct gatggaggcc aagggcgccg
     3001 aaatcgcgtt ctcgcccggc ttccgctggg gcacggcact gctgccgggc caggacatca
     3061 cgctggagca cgtgatggac cagaccgcga tcacctaccc gcaggtcacg gtgaatgagc
     3121 tgaccggtga ggcaatcaag aacctcctcg aggacgtctg cgacaacctg ttcaatcccg
     3181 acccctacta ccagcagggc ggcgacatgg tgcgcgttgg cggcatgcgc taccggtgtg
     3241 ccccgctgca gaagatcggc gcccggatca gcgacatgac cctcaagggc cagccggtcg
     3301 aggccggcaa gcgctacaag gtggcgagct gggcgccggt tgccgaaggc gtcgccgggg
     3361 agcccgtctg ggacgtggtc gccggctacc tgcgcgcgaa gaaggtggtg cgtccgttgc
     3421 agcccggccg tccggcgctt tcaggcgtgc aaggcaaccc cggcctttcc agctgatgac
     3481 cattcttgtc accggaggcg caggctacat cggctcgcat atggtgaagc tgctccggaa
     3541 gcgcggccgg cgcgtggtcg tgctcgacaa cctcgccacg gggcaccgcg atgccgtaaa
     3601 ggacgccgag ttcatcgaag gcgatgtcgg cgacaccggg aaagtcgcag cgttgctcgc
     3661 tggtcactcg gtcaaggcgg taatccattt cgcggcgtat tccctggtgg cggagtcggt
     3721 ttcgaacccg aagaaatact acgcgaacaa cgtcgatgcc acgttgagga tgctcggcgc
     3781 catgcgcgaa gcgcgcgtcg ggcggatcgt gttctcctcc accgccgcgg tatatgggga
     3841 gccgcaggcc tccccgatcg aagagtccca tccccgggag ccgatcaacc cttacggcga
     3901 gtgcaagctc gtaatcgagc gcacgctcga tgacttgcac gccgcgcatg ggttgcgctc
     3961 cgcgagcctg cgctacttca acgcgtcggg cgccgacccg gatggcagcc tcggggagcg
     4021 ccacgatccc gaaacgcacc tcattcctct cgtgctgcag gccgcttccg gccggcggcc
     4081 ggcggtcgcc gtctacggca ccgattaccc gacacgcgac ggcacctgcg tgcgcgatta
     4141 cgtgcacgtg agcgacctgt gcgacgcgca cctgctcgcg ctcgagtggc tcgagggcgg
     4201 cggcggccgc gaggccttca acctcggcag cgagagcggc gcgacggtca tggaagtgat
     4261 cgagaccgca cggcacgaaa cgggcgcgcc gatcgacgcg cgcattgaag gccgccgtcc
     4321 cggagacccc gccgtgctcg tcgcgagctg cgagcaggct cgccgcgtgc tgggctggag
     4381 cccgaagcgc tccgacctgg cgaccatcgt gcaggacgcg tggcgctggg aacgcggccg
     4441 cacggcaaat cacgccgcct gagatccggc acaggtccgg cccacatgaa ccgttcgcgc
     4501 gtggcggcgt tctccgcaat gctgcttgcg gcatgcgcga cgctcagcgg cccggattac
     4561 cgcaaccccg tgctcgatgc ggacttcccg gaccccgcgg tgctgcgcgc tcccgacggc
     4621 tggttttacg cctacgcgac ccagggcagc gttggcgaac ggatgctcaa cgtccaggtt
     4681 gcgcggtcgc gcgatctcgc gcgctgggaa cacctgggcg acgcgctgcc cggcaagccg
     4741 cgctgggccg cccgcaagca ggcgttctgg gcgccccacg tcctctacga ccggcagctg
     4801 aaccgctact acatgtatta cgcggcggag cccgatgact ccaccggaaa gtgcctgggc
     4861 gtagccaccg cgccggaccc gcgcggtccg ttcgccgact ccggcgcacc gctgatctgt
     4921 ggcgaagggc tcgagcacat cgacccgatg gccttcgatg acccccggac cggcacgcgc
     4981 ctgctgttat ggggctcggg cggcatgcct atccgcgccc gggaactggc cacggaccgg
     5041 ctgcgcttcc tgcccggcag cgcctccgtt gatctggtcg cgccggacgc cgacaggccg
     5101 taccgcgcgc tcatcgaagg cgcgtggatg acctaccggg acgggtggta ctacctctac
     5161 ttctccggcg accgctgctg cacgcgcgag ccgcgctacg ccgtgatggt ggcgcgagcg
     5221 cgccggccgc tcggtccttt cgagcactat ccgggcggcc ggggcggcag cggcgccatc
     5281 ctggaagcgg acgagcgctg gctcgccccc gggcacaaca gcatagccag cgacgcggcc
     5341 ggcaacgact ggatcgtata ccacgcctat catgcgcggc gttttcccgc tggcgatcgc
     5401 cggcgtgtaa tgctgattgg ccgtatcctt tatcgcgatg gatggccaac ggtgtcaggg
     5461 atcccgtcaa gctgagtgcg cgatggagag agtcgggctc ggatatactc gaatgctgac
     5521 gacgaggaga ttcgcctgtc atgcggccgg ataagactgg acatctgctg aaggggaaga
     5581 tgtgacatct gctcttgtgc tgggtggcgc gggttttata ggtgcgcacc tcgccgaagt
     5641 tctcttacag gcaggacatc acgtcaggat ttttgatcgt ccgcgtgttg acggccttgc
     5701 gctgcttgag gatcgcgcgg ggccggaaat tgtcactgga gactttttga accccgaaga
//
LOCUS       DBNGSG010000029         4457 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_857810, whole genome shotgun sequence.
ACCESSION   DBNGSG010000029 DBNGSG010000000
VERSION     DBNGSG010000029.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_857810"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="AC158Q_00640"
     CDS             <1..675
                     /locus_tag="AC158Q_00640"
                     /inference="COORDINATES: protein motif:HMM:NF014874.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate ligase domain-containing protein"
                     /protein_id="HIR3338304.1"
                     /translation="IIEFGLSRPAAVSARYALRWLESEIVVRTPAGEAQAVLKAPGLH
                     NVRNALAASAAAVALRVPAPAIARGLERYAGIKGRLQKKAGLNGATLIDDTYNANPES
                     VRAAIAVLAQAPGEKLLVLGDMGELGPDAPELHAEAGRHAREQGVGRLLTLGEASVHA
                     ARAFGAGARHFARIEDLLAEAGNALAPGVTALIKGSRFMRMERVVNAFALDGEEKGAR
                     REGAQP"
     gene            740..1825
                     /gene="mraY"
                     /locus_tag="AC158Q_00645"
     CDS             740..1825
                     /gene="mraY"
                     /locus_tag="AC158Q_00645"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIR3338305.1"
                     /translation="MLLELAQWLAKDIRAFNVLSYITLRAVLACLTALVISFVIGPAM
                     IRKLAAYKVGQAVRDDGPQTHLVKAGTPTMGGALILASITITALLWADLENRLVWVVL
                     WVMLGFGAIGWVDDYRKVVARNPRGLSARVKFFWQSVIGVTAACFLAFYTSLPAQTQF
                     IVPFFKEVAYPLGAVGFIVMTYFVIVGTSNAVNLTDGLDGLAIMPTVMIGSALGIFAY
                     VAGHAVFSKYLGFPYIPGAGELTVICGAIAGAGLAFLWFNAYPAEVFMGDVGALALGA
                     ALGTIAVIVRQEIVLFIMGGVFVVETLSVMLQVASFKLTGKRVFRMAPLHHHYELKGW
                     KENQVVVRFWIITMMLVLVGLSTLKLR"
     gene            1924..3318
                     /gene="murD"
                     /locus_tag="AC158Q_00650"
     CDS             1924..3318
                     /gene="murD"
                     /locus_tag="AC158Q_00650"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HIR3338306.1"
                     /translation="MDLNGRKVVVLGLGDTGLSMARWLARRKAQVRVADSRAAPPHAQ
                     TLARDLPGVALATGRFSDATLAGADLIAISPGIDRRETPIADAIRRGVPVAGDVELFA
                     QALRTLIPHPSSPIPPKVLAITGSNGKSTVTAMTGEMCRAAGRATVVAGNIGVPVLDA
                     LAAIEEGGAAPDVFVLELSSFQLESTQSLEPDAATVLNITEDHLDRYDGMADYAAAKA
                     RVFAGGGAQILNRDDRLSLAMARPGRVLHTFGIGPPRAESEWGVTPGDARALAQGARR
                     LMAVAELPLVGLHNAANALAAHALAHAIGLPDAPLIDALRKFRGLPHRMRKVAEIAGV
                     SWYDDSKGTNVGATVAALCGMERPAVLIAGGDGKGQDFSPLAPAVKARARAVVLIGRD
                     AALIERAIAGSGVPIERAGDMDDAVARASGAARRGDAVLLSPACASYDMFRDYVQRAE
                     VFAAAVRRLESGGR"
     gene            3321..>4457
                     /gene="ftsW"
                     /locus_tag="AC158Q_00655"
     CDS             3321..>4457
                     /gene="ftsW"
                     /locus_tag="AC158Q_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HIR3338307.1"
                     /translation="MFYTEAKPRVPIAEFDQGLVWTAVLLVGLGLVMVYSASIAIAEG
                     SRFTGNQPSYYLLRHGLFVGAGVMAAVVAFQIPLRLWQLAAPYLFLLGAALLVLVLVP
                     GIGREVNGSQRWISLGFVTMQPSEFMKLAAVLYAADYTARKAAHMASFRKGFLPLFVV
                     MMGVGWLLLREPDFGAFVVITVIAMGILFLGGMNWKLFAGLLSLLLAGFVLLILTSPY
                     RLQRVIGFMDPWADPYGKGYQLSHALIAFGRGEWLGVGLGASVEKLFYLPEAHTDFLL
                     AVIAEELGFVGVAAIIAAFIWIVARAFMIGRRAASLERHFSALAAQGIGLWIGVQAII
                     NIGVNTGMLPTKGLTLPLVSFGGSALVATCCALAVLLRVDWENRQ"
BASE COUNT          642 a         1465 c         1585 g          765 t
ORIGIN      
        1 atcatcgagt tcggactctc ccgtcctgcg gcggtgagcg cgcgctacgc gctgcgctgg
       61 ctggagagcg agattgtggt gcgcaccccg gcgggggagg cccaggccgt tctgaaggcg
      121 ccggggctgc acaacgtgag gaacgcgctc gcggcgagcg ccgccgcggt ggcgctgcgg
      181 gtgccggcgc cggcgatcgc gcgcgggctc gagcgctacg ccggcatcaa gggacggctg
      241 cagaaaaagg ccggactgaa cggcgccacg ctgatcgacg acacctacaa cgccaacccg
      301 gaatccgtgc gcgcggcgat cgccgtgctc gcgcaggcgc cgggagaaaa gctgctggtg
      361 ctgggcgaca tgggggaact gggccccgac gcccccgagt tgcacgccga ggccgggcgc
      421 cacgcgcggg agcagggcgt tggccggctg ctcacgctcg gagaggcgtc tgtccacgcg
      481 gcgcgcgcgt tcggagccgg cgcgcggcat ttcgcgcgaa tcgaggattt gctggccgag
      541 gccggcaacg cgctcgcgcc cggcgtcacg gcgctcatca agggctcgcg cttcatgcgg
      601 atggagcgcg tcgtcaatgc cttcgccctt gatggcgagg agaaaggggc gagaagagag
      661 ggggcccaac cgtgagcctg atctgcccgg acgcctcacg ccttgctcct cactgctcac
      721 tcgcaaacgg agattgcgaa tgctgctaga gctcgcccag tggctcgcaa aggacatccg
      781 tgcgttcaac gtcttgagct acatcacgct gcgtgcggtg ctggcatgcc tcaccgccct
      841 cgtcatctcc ttcgtcatcg ggccggcgat gatccgcaag ctcgccgcct acaaggtcgg
      901 ccaggccgtg cgcgacgacg gaccgcagac ccacctcgtc aaggccggca cgccgaccat
      961 gggcggcgcg ctgatcctgg cgtcgatcac catcacggcg ctgctgtggg ccgacctgga
     1021 gaaccgcctg gtctgggtcg tgctgtgggt gatgctcgga ttcggcgcga tcggctgggt
     1081 ggatgactac cgcaaggtgg tggcgcgcaa ccccagggga ttgtcggccc gggtgaaatt
     1141 cttctggcag tcggtgatcg gcgtcaccgc ggcgtgcttc ctcgcgttct acaccagcct
     1201 gccggcgcag acccagttca tcgtgccgtt cttcaaggaa gtcgcctatc ccctcggcgc
     1261 ggtcggcttc atcgtcatga cctatttcgt gatcgtcggc accagcaacg cggtgaacct
     1321 gaccgacggc ctcgacgggc ttgccatcat gcccacggtg atgatcggca gcgcgctcgg
     1381 cattttcgcc tacgtcgccg gccacgcggt gttctcgaag tacctcggct ttccttacat
     1441 ccccggcgcc ggcgaattga cggtgatctg cggcgcgatc gcgggcgcgg gcctcgcttt
     1501 cctgtggttc aacgcttacc cggcggaagt cttcatgggg gacgtcgggg cgctcgcgct
     1561 cggcgccgcg ctcggcacca ttgccgtgat cgtgcggcag gagatcgtcc ttttcatcat
     1621 gggcggggtg ttcgtggtgg aaacgctgtc ggtgatgctg caggttgcct cgttcaagct
     1681 gacgggcaaa cgcgtcttcc gcatggcgcc gctgcaccat cactacgagc tgaagggctg
     1741 gaaagaaaac caggtcgtcg ttcgcttctg gatcatcacg atgatgctgg ttctggtcgg
     1801 gctgtcgacg ctgaagctac gttaaatgaa accgcagata aacgcagatg aacgcggatc
     1861 gaagatcgtt cggaacctcg atatcggcgt taatcggcgt taatcggcgg ttggatatga
     1921 cttgtggatt tgaacggacg aaaggtggtc gtgctcggac tcggtgacac ggggctgtcg
     1981 atggcgcgct ggctcgcccg ccgcaaggcg caggtgcggg tggcggacag ccgcgccgcg
     2041 cccccgcacg cgcaaacgct cgcgcgcgac ctgccgggcg ttgcgctcgc gacgggcagg
     2101 ttcagcgacg cgacgctcgc gggcgccgac ctgatcgcga tcagccccgg catcgaccgg
     2161 cgcgagacgc cgatcgccga cgcgatcagg cgcggcgtgc cggttgccgg cgacgttgag
     2221 ttgttcgcgc aagcgctgag gaccctcatc cctcatccct catcccccat ccctcccaag
     2281 gtgctggcga tcaccggctc caacggcaag agcaccgtca cggcgatgac gggcgagatg
     2341 tgccgcgcgg cgggacgcgc gacggtggtg gccggcaaca tcggcgtgcc ggtgctcgat
     2401 gcgctggccg cgatcgagga aggcggcgcc gctccggatg tgttcgtgct cgaactctcc
     2461 agcttccagc tcgagagcac gcaaagcctc gagcccgatg ccgcgaccgt cctgaacatc
     2521 accgaggacc acctcgaccg ctacgacggc atggccgatt acgcggccgc caaagcccgc
     2581 gtgttcgcgg gcggcggcgc gcagatactc aaccgcgacg accgcctgag cctcgcgatg
     2641 gcgcgtcccg gccgtgtgct tcacactttt ggcatcgggc cgccgcgcgc agagtccgag
     2701 tggggcgtca cgcccgggga tgcccgcgcg ctggcgcagg gcgcgcgccg cctgatggcc
     2761 gtggccgagc tgccgctcgt gggcctgcac aatgccgcca acgcgctcgc cgcccacgcg
     2821 cttgcgcacg cgatcggcct gcccgacgcg ccgctgatcg acgcgctgcg gaaattccgc
     2881 ggcctgccgc accgcatgcg caaggtggcc gagatcgcgg gcgtgtcctg gtacgacgat
     2941 tccaagggca ccaacgtcgg cgccacggtg gccgcgctct gcggcatgga gcggccggcg
     3001 gtgctgatcg ccggcggcga cggcaagggc caggatttct ccccgctcgc gcccgccgtg
     3061 aaagcgcgcg cgcgcgccgt ggtgctgatc gggcgcgatg ccgcgctgat cgagcgcgcg
     3121 atcgccggca gcggcgtgcc gatcgagcgc gccggcgaca tggacgacgc ggtggcccgg
     3181 gcatccggcg ccgcgcggcg gggcgacgcc gtgctgctgt cgcccgcgtg cgcgagctac
     3241 gacatgttcc gcgattacgt gcagcgcgcg gaagttttcg ccgcggcggt gcggcgtctc
     3301 gagagcgggg ggcgctaggc atgttctaca cggaggccaa gccgcgcgtg ccgatcgccg
     3361 aattcgacca gggcctggtc tggaccgccg tgctgctggt cgggctgggg ctcgtgatgg
     3421 tgtattcggc gtcgatcgcg atcgccgagg gcagccgctt caccggcaat caaccgagct
     3481 attacctgct gcggcacgga ttgttcgtgg gggcgggcgt gatggcggcg gtagtcgcgt
     3541 tccagatacc gctgcgcctg tggcagctgg cggcgccgta cctgttcctg ctgggcgccg
     3601 cgctgctggt gctggtgctg gtgccgggca tcgggcggga ggtgaatggc agccagcgct
     3661 ggatctcgct cggcttcgtg acgatgcagc cctccgagtt catgaagctc gccgcggtgc
     3721 tctacgccgc cgactacacg gcgagaaaag ccgcgcacat ggcgagcttc cgcaagggct
     3781 tcctgccgct gttcgtggtg atgatggggg tcgggtggct gctgctgcgc gagcccgatt
     3841 tcggggcctt cgtcgtgatc accgtgattg ccatgggcat cctgtttctc ggcggcatga
     3901 actggaaact gttcgcgggg ctgctcagcc tgctgctggc cggtttcgtg ttgctgatcc
     3961 tcacttcccc gtaccgcctg cagcgcgtga tcgggttcat ggacccgtgg gccgatccct
     4021 acggcaaggg ctaccagctg tcgcacgcgc tgatcgcgtt cggccggggc gagtggctgg
     4081 gcgtgggact cggcgcaagc gtggaaaagc tgttctatct gcccgaagcg cataccgatt
     4141 tcctgctcgc cgtgattgcc gaggagctcg gcttcgtggg cgtcgccgcc atcatcgccg
     4201 cgttcatctg gatcgttgcg cgcgcgttca tgatcgggcg gcgggccgcg tcgctcgagc
     4261 gccatttctc cgcgctggcg gcacagggca tcggcctgtg gatcggcgtg caggcgatca
     4321 tcaatatcgg cgtcaacacc gggatgctgc cgaccaaggg actgacgctt ccgctcgttt
     4381 ctttcggcgg cagcgcgctg gtcgccacct gctgcgcgct ggcagtactg ctgcgggtgg
     4441 actgggagaa ccgccag
//
LOCUS       DBNGSG010000030         6729 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1367357, whole genome shotgun sequence.
ACCESSION   DBNGSG010000030 DBNGSG010000000
VERSION     DBNGSG010000030.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6729)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6729)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6729
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1367357"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="AC158Q_00660"
     CDS             <1..568
                     /locus_tag="AC158Q_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR3338308.1"
                     /translation="PYSRGVIAMGERDFHPLSANFLLPHWLPSHWANYAEGKRRAGTA
                     ANPADWRIARTIFVADDDKIAERYGKSDANSPYRFYWRQLRIKLTIPKRHIVFKTHEA
                     QDDAELTDDYVVDRLVICGTVNKVVDAILKLREQAGDFGELVYAGMDWVDPALARRSM
                     ELMANEVMPRVNQAIAAAPRATAATHDP"
     gene            630..1616
                     /locus_tag="AC158Q_00665"
     CDS             630..1616
                     /locus_tag="AC158Q_00665"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3338309.1"
                     /translation="MKSRRGSVRPLAVWLALAVSAPVAAPAAAQAFPSRPIRLIVAFA
                     PGGSVDLVSRLVAQKAGETLGQTIVIDNRPGAGGALSAEMAANATPDGYTIHITSASL
                     VVNVALGRKLGYDPVKSYAPVTLLASTQNALAATPALPAKGVRELIELARRQPGKINY
                     GSTGVGTSGHLTAEMFRSKAGIELTHVPYKAIGQTYTDLMSGRVSLFFPTLPGALPHY
                     RAGRIRLLGVAGAKRSAALPDVPTIAESGLPGFEASTWYPILAPAGTPKAAISRLNRD
                     FVAALKAPEIAQRLDEMAVEAVGSTPAQLTAQIKAELVKWAEVVKIAGVRTE"
     gene            complement(1736..2632)
                     /locus_tag="AC158Q_00670"
     CDS             complement(1736..2632)
                     /locus_tag="AC158Q_00670"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3338310.1"
                     /translation="MTPHSEREGAPAFRAPAFSCDSHFHVFGRPGQYPYGKDIRYTPP
                     HAPLEDFLELARHLGMERYVFVQPSAYGRNNDCMLDAMREVGTKNCRGIVDIDQDAPD
                     SEFERLNQIGVRGIRINENPIKPFEPGYSKTMLSRIERLDARCAEIGWMLDFLTPGWL
                     TLELLPVMRKLKSLHTVAHMGMFLAKDGPHQPGFRQFIDYLRGGDKCWVKFTGTYRMS
                     VAPGFADAAPMARALIEAVPDRIIWGSDYPHLSFADKVTSTGLFNLLGQWAPDEATRR
                     KILADNPQRLFGFQQGVEKHVT"
     gene            complement(2659..3456)
                     /locus_tag="AC158Q_00675"
     CDS             complement(2659..3456)
                     /locus_tag="AC158Q_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIR3338311.1"
                     /translation="MSKPPRKRTRLSSVANAMRLLKVFSDDEFEVGVSDLARRLGLAK
                     STVHRLASTLARERILERSPRDGKYRLGLLLFELGSLVRRKMDVSAEARPQLKSLMER
                     TGETVHLAVLDDHSVLYVNRIESRRAIRMGFGLGVRAPLHCTALGKALLAFQPAEFIE
                     ETIAKGLPRRTAATITSPQAFRQELAAIRARGYAIEDEEIEAGLRSIATVVRNDTGNV
                     IASIGIAGPAHRLAKKVLLSYARELMAATDAVSQRLGYQPAPAQKRA"
     gene            3583..5040
                     /locus_tag="AC158Q_00680"
     CDS             3583..5040
                     /locus_tag="AC158Q_00680"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HIR3338312.1"
                     /translation="MTTGNGATFQNDLREFITAVEKRGELARVSNAHWDRELGAVTEV
                     LYRQKVEKSPMLLFDDIPDYPRGYRCAYGMFGSPFRLSLVLGMEPSLSDNRKDMLDHY
                     RKHIRKGFKRIAPKIVKDGPVLENIVRDGNIDMLKFPVPIHHEDDGGRYIGTACGVVT
                     RDPDTGRVNVGTYRVQVKGPDRCSSYISNGKQGRIHRDKYLKAGKPCPVVIIVGTDPV
                     TYLAAGYTMPDSMPEYEWAGGLMDRPMDLIEGELTGLPFPARSEIVMEGEISATETTL
                     EGPFGEWHGYYAGDAQHEPIIHIKRIYHRTNPILTCAASQKPPHSHLFERCFLRSAAL
                     LDALDGAGIPDVKGAWLHQAGAGRTFCVVSIRQRYFGHSTQVGLVASQVASVGYVSRW
                     IVVVDDDIDPTDIHDVVWAMGTRCDPKTRTTVLDHCWSSRLDTLVTDKSMPYNTRMVI
                     DACRPYERLDTFPKVCQSSPELAAKVRARYPELYR"
     gene            5129..>6729
                     /locus_tag="AC158Q_00685"
     CDS             5129..>6729
                     /locus_tag="AC158Q_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HIR3338313.1"
                     /translation="MAEKDDAPKKTGHNLTDHQYGADLGHTHEGDGAFDHDHDHDDFG
                     EDGSPENNPLWIQDHVSLTSVGMDIGSSGTQLIFSRIHLRRLGEDLSSRYCVVSRETL
                     FQSPVALTPYKDQALIDEEKLADIIDEAYAQSGLHPDQIDSGVVILTGEALRRQNAQA
                     ISELLAENGGEFVCAAAGHHLEAMLAAYGSGAARISHDEGKRILNVDIGGGTTKLAVV
                     EGGRIAAMAAVHIGGRLQVVDEQERIVRLDPAGRHLAAKAGYAWNTGDRAPRADMERV
                     AAWMADALIAAIHARPLPHAVEHLYLTDPIAELGRIDGVMFSGGVGEYVYGREARDFG
                     DMGKLLGTAIAQRIAQGALPWPLLPAGECIRATAVGASEYSVQLSGNTIYISSPGELL
                     PRKNLQVLQPPYACGEEIDAAALASAIRGHFTAFDLEEGASEVALAFRWAGAPAYPRI
                     AAFAQGIVAGLPRTIASGKPLYLVLDGDIAQTLGAILREELKLENEILVIDGVTLWDL
                     DYIDLGRIRLPSHTVPVTIKSLLFS"
BASE COUNT         1185 a         2280 c         2220 g         1044 t
ORIGIN      
        1 gccgtactcc cggggcgtga tcgcgatggg cgagcgcgac ttccacccgc tctccgccaa
       61 cttcctgctg ccgcactggc tgccgagcca ctgggcgaac tacgccgagg gcaagcgccg
      121 cgcgggaaca gccgcaaacc cggccgactg gcgcatcgcg cgcacgatct tcgtcgcaga
      181 cgacgacaag atcgccgagc gctacggcaa aagcgacgcc aacagcccct accgcttcta
      241 ctggcggcag ctgcgcatca agctgacgat cccgaagcgt cacatcgtgt tcaagacgca
      301 cgaggcgcag gacgacgccg aactcaccga cgactacgta gtggaccggc tcgtgatctg
      361 cggcaccgtg aacaaggtgg tggacgcgat cctgaaattg cgcgagcagg ccggcgactt
      421 cggtgagctg gtctacgccg gcatggactg ggtggacccg gcgctcgcgc gccgctcgat
      481 ggaactgatg gccaacgagg tcatgccgcg cgtcaaccag gccatcgccg ctgcgccgcg
      541 cgcgaccgcc gcgactcacg acccatgaca cgcggcggtg gactggtgtc atttccccgg
      601 agcgggattt ccgaaatcga ggaggaaaca tgaaatcgag acgcggatcg gtccgcccgc
      661 tggcggtatg gcttgcactg gctgtctccg cgccggtggc cgcgccggcg gccgcccagg
      721 cgttcccgtc gcgcccgatc cgcctgatcg tggccttcgc gccgggcggc agcgtggatc
      781 tcgtctcgcg gctcgtcgcg cagaaggccg gggaaacgct cggccagacc atcgtcatcg
      841 acaaccgccc cggcgcgggc ggcgccctct ccgccgaaat ggcggcgaac gcaacgcccg
      901 acgggtacac gatacatatc accagcgcct cgctggtcgt caacgtggcc ttgggacgca
      961 agctcggtta cgacccggtg aagtcctatg cgccggtcac gttgctcgcc tccacgcaga
     1021 acgcgctggc agccacgccg gccctgccgg cgaagggggt gcgggagctg atcgagctcg
     1081 ccaggcggca gccggggaag atcaattacg gctcgaccgg cgtgggaacc tcggggcacc
     1141 tgaccgcgga aatgttccgc agcaaggcgg gaatcgagct gacccacgtg ccgtacaagg
     1201 cgatcgggca gacatatacc gacctgatgt cgggacgcgt ttcgctgttc tttcccacgc
     1261 tgccgggtgc gctgccgcac taccgggcgg gccggatacg cctgctgggt gtcgccggcg
     1321 caaagcgctc ggcagcgctg cccgacgttc ccacgattgc ggaatccgga ctgccgggct
     1381 tcgaagcgtc cacgtggtac ccgatactcg cgccggccgg cacgccgaag gccgcgatca
     1441 gcagactcaa ccgggacttc gtcgccgccc tgaaggcgcc ggaaatcgcg caacgcctgg
     1501 acgagatggc ggtggaggcg gtcggctcca ctcccgcgca gctgaccgcg cagatcaagg
     1561 ccgagctggt gaagtgggcg gaggtggtga aaatcgccgg cgtgcggacg gaataagcta
     1621 gcaggctatt gaaaaacagc tgtcgctccc gcgaaggcgg gagtccagaa aacctgcctt
     1681 agtacgattc aacgagttac tggattcccg cctccgcggg aatgacgttg ttgctctacg
     1741 taacgtgttt ttcaacaccc tgctggaagc cgaacagccg ctgcgggttg tccgcgagga
     1801 tcttgcggcg cgtcgcctcg tccggcgccc actggccgag caggttgaag agcccggtcg
     1861 aggtcacctt gtcggcgaac gacaggtgcg ggtagtcgct gccccagatg atgcggtcag
     1921 gcaccgcctc gatcagcgcg cgcgccatcg gcgcggcatc cgcgaatccc ggggcgaccg
     1981 acatgcggta ggtgccggtg aatttcaccc agcacttgtc gccgccgcgc aggtaatcga
     2041 tgaattgccg gaaacccggc tggtgcggcc cgtctttcgc cagaaacatc cccatgtgcg
     2101 cgaccgtgtg gagcgatttg agcttcctca tcaccggcag cagctccagc gtaagccagc
     2161 cgggcgtgag gaaatcgagc atccagccga tctcggcaca gcgcgcatcg aggcgctcga
     2221 tgcgggacag catggtcttc gaatagcccg gttcgaacgg cttgatcggg ttctcgttga
     2281 tgcggatgcc gcgcacgcca atctggttca gccgctcgaa ttcggagtcc ggcgcgtcct
     2341 ggtcgatgtc cacgatgccg cggcagtttt tagtgcccac ctcgcgcatg gcgtccagca
     2401 tgcagtcgtt gttgcggccg taggcgctgg gctgcacgaa gacatagcgt tccatcccga
     2461 gatggcgcgc gagctcgagg aagtcttcca gcggcgcgtg cggcggcgtg tagcggatgt
     2521 ccttgccgta gggatactgg ccgggccgcc cgaatacgtg gaaatgactg tcgcaggaaa
     2581 aagcgggcgc tctgaaggcg ggcgcgcctt ccctttctga atggggggtc aaaacaggct
     2641 cccgcaggaa atcgaaaatt acgcccgctt ctgcgctggc gcgggctgat agccaagccg
     2701 ctgggaaacc gcgtcggtcg ccgccatcaa ttcgcgcgcg taggatagca gcactttctt
     2761 cgccaggcga tgcgccggcc cggcgatgcc gatggaggcg atcacgtttc cggtgtcgtt
     2821 ccgcaccacc gtggcgatgc tgcgcagccc ggcctcgatt tcctcgtcct cgatcgcgta
     2881 gccgcgcgcg cggatcgccg ccagttcctg gcggaacgcc tgcgggctgg tgatcgtcgc
     2941 cgccgtgcgg cggggcagcc ctttcgcgat ggtctcctcg atgaactccg caggctggaa
     3001 cgcgagcagc gccttgccga gcgccgtgca atgcagcggc gcgcgcacgc cgaggccgaa
     3061 gcccatgcgg atggcgcggc ggctctcgat gcggttcacg tagagcacgg agtgatcgtc
     3121 gagcacggcg aggtgaaccg tctcgccggt gcgttccatc agcgatttca actgcgggcg
     3181 cgcttccgcg ctgacgtcca tcttgcgccg caccagggag cccagctcga acagcaggag
     3241 cccgaggcgg tattttccgt cgcgcgggct gcgctccagg attctttccc gggcgagggt
     3301 cgaggcgagg cggtgaaccg tgctcttggc cagccccagg cgcctggcga ggtcgctcac
     3361 gccgacctcg aactcgtcgt cggaaaaaac cttgagcagc cgcatcgcgt tggcgaccga
     3421 ggacaggcgc gtgcgcttgc ggggaggttt cgacatgaaa aaacggcacg ggaaattgac
     3481 ggtgaaaata ttgtatgttc aactggcagc gggttgcaac gagtaaaagg aactgcgttc
     3541 cgcatgtaag aacagggcgg cgcgcacgga aacggaggct ccatgacgac cggcaacggc
     3601 gcgaccttcc agaacgacct gcgtgaattc atcaccgccg tcgagaagcg tggcgagctc
     3661 gcccgcgtga gcaacgcgca ctgggaccgc gagctgggcg cggtgaccga ggtgctctac
     3721 cgccagaagg tggagaagtc cccgatgctg ctgttcgacg acatccccga ttatccccgg
     3781 ggctaccgct gcgcttacgg catgttcggc tcgccgttcc ggctgtcgct ggtgctcggc
     3841 atggagccgt cgctctccga caaccgcaag gacatgctcg accactaccg caagcacatc
     3901 aggaagggct tcaagcgcat cgcgccgaag atcgtgaagg acggcccggt gctcgagaac
     3961 atcgtgcgcg acgggaacat cgacatgctg aagttcccgg tgccgatcca ccacgaggac
     4021 gacggcgggc gctacatcgg caccgcgtgc ggcgtcgtca cgcgcgaccc ggacaccggg
     4081 cgcgtcaacg tcggcactta ccgcgtgcaa gtgaaggggc cggaccggtg ctcctcctac
     4141 atctccaacg gcaagcaggg ccgcatccac cgcgacaagt acctgaaggc cggcaagccc
     4201 tgccccgtcg tcatcatcgt cggcaccgat cccgtcacct acctcgccgc cggctacacc
     4261 atgcccgact cgatgccgga gtacgaatgg gccggcgggc tgatggaccg gccgatggac
     4321 ctcatcgagg gcgagctgac cggcctgccc ttccccgcgc gctccgagat cgtcatggaa
     4381 ggcgagattt ccgccaccga gaccacgctc gaggggccgt tcggggaatg gcacgggtat
     4441 tatgccggcg acgcgcagca cgagccgatc atccatatca agcgcatcta tcaccgcacg
     4501 aacccgatcc tcacctgcgc agcgagccag aagccgccgc actcgcatct gttcgaacgc
     4561 tgcttcctgc gctcggccgc gctgctcgac gcgctcgacg gcgccggcat ccccgacgtc
     4621 aagggcgcct ggctgcacca ggccggcgcc ggacgcacgt tctgcgtggt ctctatccgg
     4681 cagcgctact tcgggcactc gacccaggtc gggttggtcg cctcgcaggt ggcttcggtc
     4741 ggctacgtca gccgctggat cgtggtggtg gacgacgaca tcgaccccac cgacatccac
     4801 gacgtggtct gggcgatggg cacgcgctgc gatccgaaaa cgcgcacgac ggtgctcgac
     4861 cactgctggt cgtcgcggct cgacacgctg gtcaccgaca agtcgatgcc ctacaacacg
     4921 cgcatggtga tcgacgcctg ccgcccctac gagcggctcg ataccttccc caaggtgtgc
     4981 cagagctcgc cggagctggc ggcgaaagtc cgcgccaggt atcccgagct gtaccgttaa
     5041 ggagccccgc gtcatccccg cgcaggcggg gatccagaaa aacccgttaa cctagattcc
     5101 cgcctccgcg ggaatgacaa gatagagcat ggctgaaaaa gacgacgcgc cgaagaaaac
     5161 cggtcacaac ctgaccgatc atcagtacgg cgcggacctt ggccacacgc acgagggcga
     5221 cggggcgttc gaccacgacc acgatcacga cgacttcggc gaggacggct cgccggaaaa
     5281 caacccgctg tggatccagg accacgtctc gctgaccagc gtcggcatgg acatcggctc
     5341 ctcgggcacg caactcatct tctcgcgcat ccacctgcgg cgcctgggcg aggacttgtc
     5401 cagccgctac tgcgtggtct cgcgcgagac cctgttccag tcgccggtcg cgctgacccc
     5461 atacaaggac caggcgctca tcgacgagga gaagctcgcc gacatcatcg acgaggccta
     5521 cgcgcagtcg gggctgcacc ccgaccagat cgattccggc gtggtgatcc tcaccggcga
     5581 ggcactgcgc cggcagaacg cgcaggcgat ctcggagctt ctcgccgaga acggcggcga
     5641 attcgtctgc gcggcggccg gccatcacct cgaggccatg ctcgcagcct atggctcggg
     5701 cgctgcgcgc atctcccacg acgagggcaa gcgcattctc aacgtggaca tcggcggcgg
     5761 cacgaccaag ctcgcggtgg tggaaggcgg ccgcatcgcc gccatggccg cggtgcacat
     5821 cggcgggcgg ctgcaggtgg tggacgagca ggagcgcatc gtgcgcctcg atcccgccgg
     5881 gcgccatctt gccgcgaaag cgggctacgc ctggaacacg ggagaccgcg cgccgcgcgc
     5941 ggacatggag cgcgtggcgg cctggatggc cgatgcgctg atcgcggcca tccacgcgcg
     6001 cccgctgccg cacgcggtcg agcatctcta cctcaccgac cccattgccg aactcggtcg
     6061 catcgacggc gtgatgttct cgggcggcgt cggcgagtac gtctacgggc gggaagcgcg
     6121 cgatttcggc gacatgggca agctcctcgg caccgccatc gcgcagcgca tcgcgcaagg
     6181 cgccctgccc tggccgctgc tgccggcggg cgaatgcatc cgtgccactg ccgtcggcgc
     6241 ttccgaatac agcgtgcagc tctccggcaa cacgatttac atttcgagcc cgggcgagct
     6301 cttgccgcgc aagaacctgc aggtgctgca gcccccctat gcctgcggcg aggaaatcga
     6361 cgctgcggcg ctcgcgagcg cgatccgcgg ccacttcacg gccttcgacc tcgaggaagg
     6421 cgcatccgag gtggcgctcg ccttccgctg ggccggcgcg cccgcgtacc cgcgcatcgc
     6481 cgctttcgcg caaggcatcg tcgcgggcct gccgcgcacg atcgccagcg gcaagcccct
     6541 gtacctggtg ctcgacggcg acatcgcgca gacgctcggc gcgatcctgc gcgaggagct
     6601 gaaactcgag aacgagatcc tggtgatcga cggcgtgacg ctgtgggacc tcgactacat
     6661 cgatctcggc cgcatccggc tgccctcgca caccgtgccg gtcacgatca agtcgctgct
     6721 attcagcga
//
LOCUS       DBNGSG010000031         7230 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2216485, whole genome shotgun sequence.
ACCESSION   DBNGSG010000031 DBNGSG010000000
VERSION     DBNGSG010000031.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7230)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7230)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7230
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2216485"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="AC158Q_00690"
     CDS             complement(<1..641)
                     /locus_tag="AC158Q_00690"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR3338314.1"
                     /translation="MRFDLFHEVAVPPQLGLSESDAYAGTLAQIELADRLGYGCAWLV
                     EHHFLRGYSHSSKPELLLAAAAARTNRIRLGHAVIPLPLHHPVHVAERIATLDILSAG
                     RLEVGIGRGFAPREHEVFGADPADSRGRVEEALDILLKSFERKPVTHHGRHYRFEGLD
                     ILPHVTQQPHPPLWTAAVSPDTFEWAAARGLGVLAGPFKPWFMVNHDIKRYRAA"
     gene            complement(684..1742)
                     /locus_tag="AC158Q_00695"
     CDS             complement(684..1742)
                     /locus_tag="AC158Q_00695"
                     /EC_number="2.3.1.-"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF046470.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HIR3338315.1"
                     /translation="MAETWRLIDTGLRPAAQNIALDRALLEARRAGEIPSTLRFLRFA
                     PCALIGCGQSPGQELNLEACRAGGFDIQRRLTGGDVMVLDERQLVWALYLDRHDTGTT
                     DLRTISRRLCHAAAAAVSALGVNAAYRAQNEIEVDGRRISESAGVFDGDALLFQGTLF
                     VDLDPGTLARVSRLLGAAPGAASNRVANLKELLGAKPDISLIRRNLMEAFECEFGVEF
                     GECDLTLSENTRYQAALQEIGPPDWINLVSRPAAELPLLRAQHQTAGGRLHAAIAYDR
                     PTRVIRQTWFATEVSLAPPRVLADLEAALRHVPADRLEQRIERFFAGRAVDMTPLSPA
                     DFIGAVQLALGQLLVDRV"
     gene            complement(1792..2019)
                     /locus_tag="AC158Q_00700"
     CDS             complement(1792..2019)
                     /locus_tag="AC158Q_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003263554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HIR3338316.1"
                     /translation="MHFDKELDARGLNCPLPILRTKKALTDMASGQVLKVLATDPGSV
                     KDFAAFTKQTGNTLLSSEAAGKEFVFFMEKR"
     gene            complement(2125..3462)
                     /locus_tag="AC158Q_00705"
     CDS             complement(2125..3462)
                     /locus_tag="AC158Q_00705"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3338317.1"
                     /translation="MDASRITVPGLPAVLEKVGKYEIIKELGRGATSSVYQAYDPFQR
                     RQVAIKVVFPEALGDKEHGKRYKKLFVTEASLAGKLSHPHIVAIHDAVADEDGSYIVM
                     EYVDGTTLEQYARHDALLPVQRIVEIAYKCARALEYASKQGVIHRDIKPANILLTGES
                     DIKISDFGAALTVAAETTQLSGVGSPAYMSPEQVKELPLTYQTDIFSLGVVMFQLLTG
                     RLPFKGANNYSMIYQIINVDPPRPIQVRPDIPPRVDAIVMRALEKDTTKRYQTWEEFA
                     HDLATALGKEEKSGQGFAESEKFDTLRKLEFFRQFSDVELWEVLRLASWHQRPRDTVL
                     IHENDIGSSFFILVSGEVKVVKQERLLNVLKAGECFGEMAYLGKQKFQRSASIVALSE
                     ISVIEIRAETLSKASDLCRHHFHGAFLELLVDRLTMANLRLSQLLADRNVSVY"
     gene            complement(3657..5000)
                     /gene="chrA"
                     /locus_tag="AC158Q_00710"
     CDS             complement(3657..5000)
                     /gene="chrA"
                     /locus_tag="AC158Q_00710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HIR3338318.1"
                     /translation="MDNANPNEQGRENAVSYGEALRVWFKIGCIGFGGPAGQIALMHR
                     ILVDEKKWVEESRYLHALNFCMLLPGPEAQKLATYVGWLLHGTRGGLAAGILFVAPGA
                     LVMLGVSLLYVLGRGVSVVDGALYGIKAAVLVIVVEALIRIGRRSLKTPLLMSLAGAA
                     FIGIFFFDLPFPLIIAAAAAAGFVVARSSPGLIGLKAGEVAPVAPMPGRWRQAARVTV
                     VGLALWWAPVALAAVMLGPQHVLVDIGTFFSKLAVVSFGGAYALLAYMAQEAVETYRW
                     MTAPEMVDGLGLAETLPGPLIKITQFVGFLGAYRDAAPFSPVAAAIIGSAMTTWVTFV
                     PPMLMIFAGAPFIEQLRSNQRLSGALAAITAAVVGVILNLTVWFVLHVLFTRVEEMQA
                     GPLRWYAIDPATLDIKVLALALFAAVLAFRLHRGLVEMVAIMATLGIAVRLLSGA"
     gene            complement(5161..6594)
                     /locus_tag="AC158Q_00715"
     CDS             complement(5161..6594)
                     /locus_tag="AC158Q_00715"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HIR3338319.1"
                     /translation="MELRAFLIPLLFATPGVLAQGLPELGDAAQSGFTPLQERRLGES
                     IMREIRADRTYYDDAEATDYLAGLGNRLVARSADARQEFDFFLIRDGQINAFALPGGF
                     IGVNTGLLLASQSESELASVMAHEIAHVTQRHIAGMIAQQKQSTVTALASLAAALLLS
                     RVSGDAAQAAFAFGQAGAIQSQLNFTRENERDADRAGLQILDQAGFDPHAMASFFERL
                     QRATRVYEGGAPSYLRTHPLTFERIADIQNRTQTISYRQVPDSIGFQLIRAKLRAQSG
                     APRDAVAFFEESLAERKFLSEAATRYGLAASLMRQKNYARARTEIERLRKLVPANPIV
                     ETLSCEFGLEALGPEKSLPCYREALKTYPAYRALVYGYAGALLQAKQPQDALRFVEAR
                     LQAVTDDHRLYLLQARSNAALDRRLAQHRAQAEAYARLGNLPAAIEQLQIGLKSGDGD
                     FFQLSAAEARLRELRKLDEETRRDLQR"
     gene            6613..7131
                     /gene="moaC"
                     /locus_tag="AC158Q_00720"
     CDS             6613..7131
                     /gene="moaC"
                     /locus_tag="AC158Q_00720"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HIR3338320.1"
                     /translation="MRRSSYSRLARNVKKFTHIDARGRARMVDVADKDETRRIARAGG
                     RIRMLPATLKRIVSGTARKGDVLGIARTAAIQATKRTAELIPLCHPLALTHVGVDFKV
                     SRKDSSVECVATVATIGRTGVEMEALTAVAVGLLTIYDMCKAVDRGMRLERIGLLEKR
                     GGKSGHWVATKS"
BASE COUNT         1289 a         2404 c         2335 g         1202 t
ORIGIN      
        1 gccgcgcggt agcgcttgat gtcgtgattg accatgaacc agggcttgaa cggtcccgcc
       61 agcacgccca gcccgcgggc ggcggcccac tcgaaggtgt cgggactgac cgccgccgtc
      121 cacagaggcg ggtgcggctg ctgcgtcacg tgcggcagga tgtcgagccc ctcgaagcgg
      181 tagtggcggc catggtgcgt gactggcttt ctctcgaagc tctttaacag gatgtcgagg
      241 gcctcctcaa cgcgcccgcg cgaatccgcc gggtccgcgc cgaagacctc gtgctcgcgc
      301 ggcgcgaagc cacggccgat gcccacctcc agccgcccgg ccgacaggat gtccagcgtc
      361 gcgatgcgct cggccacgtg cacggggtga tgcagcggca ggggaatgac ggcgtggccc
      421 agccggatgc ggttggtgcg cgccgccgcc gccgcgagca gcagctcggg cttcgacgaa
      481 tgcgaatagc cgcggaggaa gtgatgctcg accagccagg cgcagccata tcccagccgg
      541 tcggccagct cgatctgggc cagcgtcccg gcgtacgcat cggactcgct cagcccgagc
      601 tgcggcggca cagccacttc atgaaacagg tcgaaacgca tggaacgcgg cggcggccgg
      661 aagcggcgcg cccgcaatga ctctcagacc cggtcgacca ggagttgccc gagtgcgagc
      721 tgcaccgcac cgatgaagtc cgccggactg agcggcgtca tgtccaccgc gcggccggcg
      781 aaaaaacgct cgatcctttg ctccaagcgg tctgccggca catgacgcaa cgcggcctcg
      841 aggtccgcca gcacgcgtgg cggcgcaagt gagacctcgg tcgcaaacca agtctgccgg
      901 atgacacgcg tcgggcggtc ataagcaatc gcagcgtgca gtcgcccgcc ggccgtctga
      961 tgctgcgccc ggagcagcgg taattccgcg gccggccgcg acaccaggtt gatccaatcc
     1021 ggagggccga tctcctgcag cgccgcctgg tagcgggtgt tctcggagag cgtgagatcg
     1081 cattcgccga actcgacgcc gaactcgcat tcgaacgctt ccatcaggtt gcgccgaatc
     1141 aacgagatgt ccggcttcgc gccgagcagt tccttgagat tcgccacgcg attactcgcg
     1201 gcgccgggcg ctgcgccgag aagccgggac acgcgcgcga gtgtcccggg atcgagatcg
     1261 acgaacagag tgccctggaa cagcagtgca tcgccgtcga aaacgccggc actctccgaa
     1321 atcctgcgtc cgtccacctc gatttcattc tgcgcgcggt aggcggcatt cacgccgagc
     1381 gcgctcaccg ccgcggccgc ggcatggcac agccggcgcg aaatcgtccg caggtcggtc
     1441 gttccggtat cgtgccggtc gaggtagagc gcccagacga gctggcgttc gtcgagaacc
     1501 atgacgtcgc cgccggtcag ccggcgctgg atatcgaaac cgccggccct gcaggcctca
     1561 aggttaagtt cctgaccggg actctggccg cagccgatga gcgcacaggg tgcgaagcgc
     1621 aggaacctga gcgtgctcgg aatctccccg gcgcgacggg cttcgagcag cgcccggtcg
     1681 agcgcgatat tctgcgccgc cggccgcagc cccgtgtcga tcaggcgcca ggtctccgcc
     1741 atgctccgtc aaccatcgcg agccgccagc gttgcggccg cactgcccgc actagcgctt
     1801 ttccatgaaa aagacgaatt ccttgccggc cgcctcggac gagagcagcg tattgccggt
     1861 ctgcttggtg aacgcggcaa agtctttcac cgaccccgga tcggtcgcga gaaccttcag
     1921 gacctgcccc gaggccatgt cggtgagcgc tttcttggtg cgcaaaatcg gcagcgggca
     1981 gttcaacccg cgcgcgtcga gttccttgtc gaaatgcata gccatccccg tttcgattga
     2041 ttccgccagt atcgccggaa ctataggttc attccctggc cgccgcaaga taatcactcc
     2101 cggtaaaacc cctccgtaaa tctctcagta aacgctgacg ttgcggtcag cgagcagctg
     2161 ggacagcctc agattggcca tcgtcaggcg gtccaccagc agctcgagaa acgcgccgtg
     2221 gaagtggtga cggcacaggt ccgaggcctt ggacagggtc tcggcgcgga tctcgatcac
     2281 ggaaatctcg gacagcgcca cgatgctcgc cgagcgctgg aacttctgtt tgccaagata
     2341 cgccatctcg ccgaaacatt ccccggcctt cagcacgttc agcagccgct cctgcttcac
     2401 gaccttgacc tcgcctgaca ccaggatgaa gaacgaggaa ccgatgtcat tttcatgaat
     2461 cagcacggtg tcgcgcggac gctggtgcca ggacgcgagg cgcaacactt cccacagctc
     2521 gacgtcgctg aactgccgga aaaattcaag cttgcgtagc gtatcgaatt tctcgctttc
     2581 cgcaaagccc tgccccgact tctcttcctt gccaagcgcc gtggcgagat cgtgcgcaaa
     2641 ctcctcccag gtctgatagc gcttcgtggt gtccttctcg agcgcgcgca tcacgattgc
     2701 atcgacgcgc ggcgggatat cgggccggac ctgtatgggg cgcggcggat cgacgttgat
     2761 gatctggtag atcatgctgt agttgttggc gcccttgaac ggcagccggc cggtcagcag
     2821 ctggaacatc accacgccga gcgaaaagat atcggtctgg taggtgagcg gcagctcctt
     2881 gacctgctcc ggcgacatgt aggcgggcga gccgacgccg gagagctgcg tcgtttccgc
     2941 cgccacggtc agcgcagcgc cgaaatccga tatcttgatg tcggattctc cggtaaggag
     3001 gatgttggca ggcttgatat cgcggtggat cacgccctgc ttcgacgcgt attccagggc
     3061 gcgtgcgcac ttgtaggcga tctcgacgat acgctgcacc ggcagcaggg cgtcatgccg
     3121 cgcgtactgc tcgagcgtgg tgccgtccac gtactccatc acgatgtagc tcccgtcctc
     3181 gtccgccacc gcgtcgtgga tcgccacgat gtggggatgc gagagcttgc cggcgaggga
     3241 cgcctcggtg acgaacagct tcttgtagcg cttgccgtgc tccttgtcgc cgagcgcttc
     3301 gggaaaaacc accttgatgg cgacctggcg gcgctggaac gggtcgtacg cctggtagac
     3361 ggaactggta gcgcctctgc ccagttcctt gatgatctcg tatttcccga ccttttccag
     3421 cacggctggc aagccgggaa cggtgatccg ggatgcatcc atgcagcaag ataccataac
     3481 gattcccccc ggaagccggg cgcaaccggt tgaaatccat cacgcaaaat cgccggcggc
     3541 gaaacggcac cgcaagcccg cccggcgtga atcacgtcac gcctgcgtgc attcccgcgc
     3601 gcggtgcccg cctccgttcg tgaccgggtt ggcgattccc gtgccgttcg ccgccgtcac
     3661 gcgcccgata acagccgcac cgcaattccg agcgtggcca tgatcgccac catctcaacc
     3721 aggccgcggt gcagccggaa cgccagcacc gccgcgaaca gtgcaagcgc aagcaccttg
     3781 atatcgagcg tagcaggatc aatcgcgtac cagcgcagcg gtcccgcctg catttcctcc
     3841 acccgggtga agaggacatg cagcacgaac cagaccgtga ggttgaggat gacgccgact
     3901 accgctgcgg taatggcggc gagcgcgccc gacaggcgtt gattggaccg caactgttcg
     3961 atgaacggcg cgccggcgaa gatcatgagc atcggcggca cgaacgtgac ccacgtggtc
     4021 atcgcggacc cgatgatggc tgcggcgacc ggcgagaacg gggccgcatc gcgataggcc
     4081 ccgagaaaac ccacgaattg cgtgatcttg atgagcgggc ccggcagcgt ctcggccagc
     4141 ccgagcccat cgaccatttc cggcgcggtc atccagcgat aggtctcgac cgcttcctgg
     4201 gccatgtaag caagcagcgc gtaggcgccg ccgaaactca ccacggcaag cttcgagaag
     4261 aacgtgccga tgtcaacgag cacgtgctgc ggaccgagca tcacggcggc gagcgcaacc
     4321 ggcgcccacc acagcgcgag gccgaccacc gtgaccctgg cggcctgacg ccagcgtccg
     4381 ggcatgggcg cgaccggcgc aacctccccg gccttcagcc cgatcagccc gggggacgac
     4441 cgcgcgacca cgaagcccgc ggcggcggcc gcggcaatga tcaggggaaa cggcaggtcg
     4501 aaaaagaaga tgccgatgaa cgccgcgccc gccagggaca tcaacaacgg cgttttcaac
     4561 gagcgtcggc cgatgcgtat cagcgcttcg accacgatca cgagcacggc ggccttgatc
     4621 ccgtacaacg ctccatccac caccgagacg ccgcgcccga gcacgtagag caggctcacg
     4681 cccagcatga cgagcgcacc cggagccacg aacagtatgc cggcggcgag cccgccgcgg
     4741 gtgccgtgca gcagccaacc gacataggtg gcgagcttct gcgcttccgg accgggcagc
     4801 agcatgcaga agttgagcgc gtgcagatat cgcgactcct cgacccattt cttctcgtcg
     4861 accagaatac ggtgcatcag cgcgatctgg cccgccggac caccgaaacc gatgcagccg
     4921 atcttgaacc agacgcgcag cgcttcgcca taggacacgg cgttttcgcg gccttgctcg
     4981 ttcggatttg cgttgtccac gtcacctcct ttgcgtacgc ggtatcgcgc aaaggcagcg
     5041 cttggacgca ggacgtctgg gaatacgggg ggtctgcccg gcccccgcgt gacgggacga
     5101 tgatgcgtcg gccgctcccg agtgtcaaga tcgcgcccat tgcgcggcta cgggcggagg
     5161 ttaccgttgc agatcgcgcc gcgtttcctc gtcgagcttg cgcaattcgc gcagccgcgc
     5221 ctcggccgcc gagagctgga agaaatcgcc gtcgccgctt ttcagcccga tctgcagctg
     5281 ctcgatggcc gcgggcaaat tgcccaggcg cgcataggct tccgcctggg cgcggtgctg
     5341 ggcaaggcgc cggtcaagcg cggcattgct gcgcgcctgc agcaggtaga gccggtgatc
     5401 gtccgtgacc gcctgcagcc gcgcctcgac aaaccgcaat gcgtcctgcg gctgtttcgc
     5461 ctgcagcagc gcgccggcgt atccgtagac cagcgcgcgg taggcgggat aggttttcag
     5521 cgcctcgcgg taacagggca gcgacttttc cggtccaagc gcttcgagcc cgaattcgca
     5581 ggagagcgtt tccacgatcg ggttggccgg caccagcttg cgcagccgtt cgatctcggt
     5641 gcgcgcgcgc gcgtagttct tctgccgcat caggctcgcg gccagcccat aacgggtcgc
     5701 cgcctcgctc aggaacttgc gctcggcgag gctttcctcg aaaaaggcaa ccgcgtcgcg
     5761 cggcgcaccg gactgcgctc tcagcttggc gcggatcagc tgaaagccga tgctgtccgg
     5821 aacctggcgg tagctgatgg tttgtgtccg gttctggatg tcggcgatgc gctcgaaggt
     5881 gagcgggtgc gtgcgaaggt acgacggcgc cccgccttcg tagacgcgcg tcgcgcgctg
     5941 caggcgctcg aagaagcttg ccatggcgtg cggatcgaac cccgcctggt cgaggatctg
     6001 cagcccggcg cggtcggcgt cacgctcgtt ctcgcgcgtg aaattgagct ggctctggat
     6061 cgcgcccgcc tgcccgaaag cgaaggcggc ttgcgcagcg tcgccgctta cgcgcgatag
     6121 caacagggcc gccgccagcg acgccagcgc ggtgacggtg ctttgcttct gctgtgcgat
     6181 catgcccgcg atgtggcgct gggtgacgtg ggcgatctcg tgcgccatta cgctggccag
     6241 ttccgattcg ctttgcgagg cgaggagcag cccggtattg acgccgatga atccgcccgg
     6301 cagggcgaac gcattgatct ggccgtcgcg aatcaggaag aagtcgaatt cctggcgcgc
     6361 gtccgcgctg cgcgcgacga ggcgattgcc cagtccggcg aggtagtccg tcgcctccgc
     6421 gtcgtcgtag taggtgcggt cggcgcggat ctcgcgcatg atgctctcgc ccagccgccg
     6481 ctcctgcagc ggcgtgaagc ccgactgagc cgcgtcgcca agttcgggca acccctgggc
     6541 cagcacgccg ggtgtcgcaa acaacagcgg gatcagaaat gcgcgcagct ccatgatgct
     6601 atgataattc ggatgcgcag gagttcctat tcccgcctgg cccgcaatgt gaagaagttc
     6661 acccatatcg atgcgcgcgg tcgcgcgcgc atggttgatg tggcggacaa ggacgagacg
     6721 cgccgcatcg cccgcgccgg cggccgcatc cgcatgctgc ccgcgacttt gaagcgcatc
     6781 gtttccggga ccgccaggaa aggcgatgtc ctgggcatcg cccgcaccgc cgcgatccag
     6841 gccacgaagc gcactgccga gctgatcccg ctttgccacc cgctcgcgct cacgcatgtc
     6901 ggcgtcgact tcaaggtcag ccgcaaagat tcatcggtgg agtgcgtggc aacagtcgcc
     6961 accatcggac gcaccggagt cgagatggaa gcgctcaccg ccgtcgcggt cgggctgctc
     7021 acgatttacg acatgtgcaa ggcggtcgac cgcggcatgc ggctcgagcg catcggcctt
     7081 ctcgagaaaa ggggcgggaa atcaggtcac tgggtcgcga cgaagagttg acgctcgttg
     7141 gcctgtcatc cctcggcctg aaaccggcct gggcagttcg ctgtcttttc ctcgcagctt
     7201 tttttatggg ccgataaagt ccatccctat
//
LOCUS       DBNGSG010000032         1615 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4084955, whole genome shotgun sequence.
ACCESSION   DBNGSG010000032 DBNGSG010000000
VERSION     DBNGSG010000032.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4084955"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="AC158Q_00725"
     CDS             complement(<1..529)
                     /locus_tag="AC158Q_00725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HIR3338321.1"
                     /translation="MSKTSQREHDLLVEKDVEIPVRDGARLKADIFRPKSGGRFPAIM
                     NIGGYRKDKVWVPPADLEEKANPLMNWEAVNPLWWIPRGYIAIRVDTRGSGKSPGLFD
                     PWSRQEALDFYDCIEWAAKQKWCNGRIGTSGISYFAMNQWLVASLRPPSLKAIIPWEG
                     AADMYRDLAYHGGIFC"
     gene            complement(564..1547)
                     /locus_tag="AC158Q_00730"
     CDS             complement(564..1547)
                     /locus_tag="AC158Q_00730"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3338322.1"
                     /translation="MQINRMARLLLIVTFTAGAAISHAAERFPQRPVRLIIPYAPGGA
                     TDITARQLQAKIADRWGQPVIVDNRPGASGNIALDLAARSAPDGYTLFVGNVSTNAIN
                     ETTFSSLKVKPSRDLTGVTNLIQLPHLWVVNPGIPASSLKELVAHVKKTGAKLNYGSA
                     GVGAYPHLDAVKFLRIAGIEMTHVPYKGGAGQMIPAIMGNEVQFMFINMASSLPHIRT
                     GRIKPLAITTPARRPELPNVLTTAESGFPGVGTNAWNGLFAPAGIPKPLLARIHADVV
                     KVMESPEMKASLEKVFMSVVVNKSPQEYQQFVLKEIKDWAKIVIENNIKVE"
BASE COUNT          282 a          489 c          504 g          340 t
ORIGIN      
        1 ggcagaagat gccgccgtgg taggcgaggt cgcgatacat gtcggccgcg ccttcccacg
       61 ggatgatcgc cttgagggaa ggcgggcgca ggctggcaac cagccactgg ttcatcgcga
      121 aataggaaat gccgctggta ccgatgcgcc cgttgcacca cttctgcttc gctgcccact
      181 cgatgcaatc gtaaaaatcg agcgcctcct ggcgcgacca gggatcgaac agcccggggg
      241 acttgcccga gccccgcgtg tcgacgcgga tcgcgatgta gccgcggggt atccaccaca
      301 ggggattcac cgcttcccag ttcatcaacg gattggcttt ttcctccagg tccgccggcg
      361 gcacccacac cttgtccttg cggtaaccgc cgatgttcat gatcgcgggg aaacggccgc
      421 ccgattttgg acggaagatg tcggccttca ggcgcgcgcc gtcgcgcacc gggatctcga
      481 cgtctttctc aacgagtaga tcgtgctcgc gctgggaagt tttgctcatg attcctgttg
      541 atgaaggacg cggtccgcgt ccttcattcg accttgatgt tgttctcgat gacgatcttg
      601 gcccagtcct tgatttcctt caggacgaat tgctggtatt cctgcgggga cttgttgacc
      661 accaccgaca tgaatacttt ctccagcgag gccttcatct ccgggctctc catcactttg
      721 accacgtcgg catggatgcg ggccagtagc ggcttgggta tgcccgccgg agcgaacagg
      781 ccgttccagg cgttggtccc caccccggga aagcccgatt cagccgtggt caggacattt
      841 ggcagctcgg ggcgccgcgc gggcgtcgtg atcgccagag gcttgatccg gccggtgcga
      901 atgtgcggca gcgacgaggc catgttgatg aacatgaact ggacttcgtt gcccatgatg
      961 gcaggaatca tctgtccggc accgcccttg taaggaacgt gcgtcatctc gattccggcg
     1021 atcctgagaa acttcacggc gtcgagatga gggtacgcgc ccaccccggc ggacccatag
     1081 ttcagttttg caccggtttt cttgacgtgc gccaccagtt ccttgaggct cgaagcggga
     1141 attccgggat tcaccaccca caggtgcggc agctggatca ggttggtgac gcccgtcagg
     1201 tcgcgcgagg gcttcacttt aagcgagctg aaggtggttt cgttgatggc gttggtcgaa
     1261 acgttgccga caaagagcgt gtagccgtcc ggcgcggacc gtgcagcaag atcgagcgca
     1321 atgttgccgg aagcgcccgg acggttgtcc acgatgaccg gctggcccca gcggtcggct
     1381 attttcgcct gcagctggcg cgccgtgata tcggtcgctc cgcccggggc gtagggaatg
     1441 atgaggcgta ccggccgctg cgggaaccgc tctgccgcgt gcgatatggc ggcaccagcc
     1501 gtaaacgtga ctatgagaag cagccttgcc attctgttga tctgcatggt ttcctcctcc
     1561 ggttacgaat gcgttacgcc ccgagtcgtc tgcccaactt tcaattcacg gccgc
//
LOCUS       DBNGSG010000033         3998 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_9747, whole genome shotgun sequence.
ACCESSION   DBNGSG010000033 DBNGSG010000000
VERSION     DBNGSG010000033.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3998)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_9747"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            464..1213
                     /locus_tag="AC158Q_00735"
     CDS             464..1213
                     /locus_tag="AC158Q_00735"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR3338323.1"
                     /translation="MTVPFDLPMFAIAAAVVFGSYVVFGISAFGAALFTVPILSHFLP
                     LDFVLPMCVLLDVSAAFALGVRFSRDADRTELAWMVPFSLAGAIAGVTLLVALPRQAT
                     LAGIGAFLVAYALYSMAQRGATRMVSRAWAPVAGFVGGAFGTLFGIGAPPYAIYLAHR
                     TQDKLGYRATLSNMVIFSVGMRALVFTAGGLMLADRLIGFAMLVPFCLAGLWLGNRLQ
                     ARISRGGLLRVVSGLLLLIGVSLIVRALTVG"
     gene            complement(1422..1850)
                     /locus_tag="AC158Q_00740"
     CDS             complement(1422..1850)
                     /locus_tag="AC158Q_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338324.1"
                     /translation="MEREQTLRILNALANGVHPATGEKFAADGPYQHPDTVRALFQAM
                     RAVEGSGATVAAPAPAPERKPALPESGSGSRWSAEEEQRLAAAFDTGKTVDELARAHG
                     RSRAAIEARLVRLGKMDASAVTTPLRYPPKPAGNTARQGT"
     gene            2060..2656
                     /locus_tag="AC158Q_00745"
     CDS             2060..2656
                     /locus_tag="AC158Q_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338325.1"
                     /translation="MVTLLLARDAKVQAAAVARLALARPDCPDAEKLEAALARAAAAP
                     PGWEEALAGFASDPSPEAWRELMCFAPSEYIYQRTRDAIRRLRKMGVDGDTLFRSASE
                     FGLTPDLIDLVEDGRVTARTIIERAGRSGDARATYIGLAAEAAFLVGDMLGTVRLLRD
                     SIAHENEWCSALPHIFFVRERATVDQGKLLDRAGIPRV"
     gene            complement(2673..3872)
                     /locus_tag="AC158Q_00750"
     CDS             complement(2673..3872)
                     /locus_tag="AC158Q_00750"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing class I SAM-dependent
                     RNA methyltransferase"
                     /protein_id="HIR3338326.1"
                     /translation="MTERLFATCPRGLEQVLADELAALGARDVSPVDGGVAFGGDLAL
                     CYAANLESRVASRVLWQVGCAGYRTEQEIFSAARALAWPRFFDVRRTIRVNVSAIKSP
                     VRSLDFVTLSVKDAVCDAFRDARGSRPDVDTARPDVRIHVFLTRDQATFYLDTSGEAL
                     FKRGWRITTGDAPLKENLAAGILKLTGWKAPTPLFDPMCGSGTFLIEAAMMALDAAPG
                     LNRAFGFEKLENFDGKGWRALQDKAGARRRPLTRLPVHGSDKSGTVLGLARENLAAAG
                     IENAVQLKQMDILDGSPPAEPGIVVMNPPYGERQATEEELAAFYPRLGDALKQRYAGW
                     TAYILTADLRLARLIGLKASKRTPLFNGALECRLFEYRMIAGTMRRSKPLVTGESEDA
                     GADKNSN"
BASE COUNT          625 a         1278 c         1310 g          785 t
ORIGIN      
        1 agctgcgcct gatgatcaag ctccacggca aggaagcgca ggagaaggat ctgtcgacgg
       61 gtatcgagca cctgaacatc gtctgatcct ggacgcgaag cgccggataa gagcctctaa
      121 cctagcaaaa cgcgtgatgc gctggcacga ttttggctca gggcgcgaag caaggcccgc
      181 cacttagcca cctaagcaag ggatttgcga caaagccatg ggccaaaact cgcaccagcc
      241 cacaaggggt tgctgccaga accggcgctg actttgtcgc tcgacttgca aatatcgaca
      301 tattagctgc acctcgcttc gcgtcaccac caattctggc agcaacgcac acgtgttttg
      361 ctaggttaga ggctctaata cgccggttcg agcggaccga taccacccgc tcaaccggcg
      421 taacaaaagc gtcggatgac attccggcgc ttttttattg cacatgaccg ttccgttcga
      481 ccttccgatg ttcgccatcg ccgcggcggt ggtattcggg tcctacgtcg tcttcggcat
      541 ctcggcgttc ggggcggcgc tgttcaccgt gccgatcctg tcgcatttcc tgccgctcga
      601 tttcgtgctg ccgatgtgcg tgctgctcga cgtgtcggcg gcgttcgcgc tcggcgtgcg
      661 cttctcgcgc gacgcggacc ggaccgagct cgcctggatg gtgccgttct cgctcgccgg
      721 cgcgatcgcc ggggtgacgt tgctggtggc gctgccgcgg caggcgaccc tcgcgggcat
      781 cggcgcgttc ctggtcgcgt acgccctcta ctcgatggcg cagcgcgggg cgacccgcat
      841 ggtgagccgc gcctgggcgc cggtggccgg cttcgtgggc ggggcgttcg ggacgctttt
      901 cggaatcggc gcgccgcctt acgccattta ccttgcgcac cgcacgcagg acaagctcgg
      961 ctaccgcgcg acactgtcca acatggtgat cttctccgtc ggcatgcgcg cgctggtgtt
     1021 caccgcgggc gggctcatgc tcgccgaccg gctgatcggc ttcgccatgc tggtgccctt
     1081 ctgcctcgcc ggactgtggc tcggcaaccg cctccaggcg cggatttcgc gcggcggcct
     1141 gctgcgcgtc gtgagcggtt tgctgctgct gatcggcgtg tcgctgatcg tgcgggccct
     1201 gacagtcggt taaatatcag ccgccgatga acgccgattc acgccgatag aatcaacaaa
     1261 aatagacagg atttacagga tttctcagga ttaacaagat taaggcggac gttccaaaga
     1321 gcgttcgatt taaaaatccc attctgcaaa tccggtgtcg attacggtct tgatcttatc
     1381 tgcgtttatc ggcgttcatc ggcggttcca tgctacttat gtcacgtgcc ttggcgcgcc
     1441 gtgttcccgg cgggtttcgg gggatagcgc agcggggtag tgaccgccga ggcgtccatc
     1501 ttgccgagcc gcaccagccg cgcctcgatc gccgcgcggc tcctgccgtg ggcgcgcgcc
     1561 agttcgtcca ccgtcttgcc ggtgtcgaac gcagccgcca gccgctgctc ctcctcggcg
     1621 ctccagcgcg agccggagcc gctttccggc agcgcgggct tgcgctcggg tgcgggagcc
     1681 ggcgccgcca ccgtcgcgcc gctgccctcg accgcgcgca tggcctggaa cagcgcccgc
     1741 acggtgtcgg gatgctggta ggggccgtcg gccgcgaact tctctcccgt cgccgggtga
     1801 acgccgttcg ccaaggcgtt caggatcctg agtgtctgtt ccctctccat ggctctttcc
     1861 tccgtagttg gtatcgcgcg acgctcgtgc ggagcgcgtg gaggctagaa cgcggcgtac
     1921 gtcatggcgg ttgacggcgc taagattgtc atcctggttg tccgtggcgc gttgtaacag
     1981 aggttccatg ggtgtcgtta gagatagtga gcttcgcgaa gccgaacagc gttggctgga
     2041 cgggggcagt gcgctgcccg tggtaacgct gctgctagcg cgcgatgcca aggtgcaggc
     2101 tgcagcggtc gcgcgcctgg ctctggctcg cccggattgc ccggatgcgg agaaactgga
     2161 agcagcgctc gcccgcgcag cggcagcgcc gccgggctgg gaggaggcgc ttgccggttt
     2221 tgccagtgat ccgtccccgg aagcgtggcg agaactcatg tgtttcgcgc cgtccgagta
     2281 catctatcaa aggacacgtg acgcgatacg gcgcctgaga aagatgggcg tggacggcga
     2341 taccctgttt cgcagcgcca gtgaattcgg cctcacgccg gacctgatag atctggtgga
     2401 ggatggtcgc gtgacggcca ggacaatcat cgaacgcgcc ggccgatccg gtgacgctcg
     2461 cgccacttat attggcctgg ctgcggaggc ggcttttctc gtcggagata tgttgggcac
     2521 tgtgcggctg ctccgggatt caattgcgca cgagaatgaa tggtgttccg cattgcccca
     2581 tatcttcttt gtccgggagc gggctacggt tgaccaggga aaattgctcg atcgggcggg
     2641 cataccccgg gtctgattgc ccgagcccgg tattaattac tgttcttgtc cgctcccgcg
     2701 tcttcggact ctcctgtgac aaggggtttg ctgcgcctca tcgtcccggc aatcatccgg
     2761 tactcgaaca gccggcactc cagcgcgccg ttgaatagcg gcgtgcgttt ggaggccttg
     2821 aggccgatca gccgggcgag gcgcaggtcg gcggtgagga tgtaggcggt ccagccggcg
     2881 tagcgctgct tgagcgcatc gccgagcctg gggtagaagg cggccagctc ttcttccgtt
     2941 gcctggcgct cgccgtaagg cgggttcatc accacgatgc cgggctccgc gggcgggctg
     3001 ccgtcgagga tgtccatctg cttgagctga accgcgttct cgattcccgc cgccgcgaga
     3061 ttctcgcgcg cgagaccgag gacggtcccg gacttgtcgc tgccatggac cggcagccgg
     3121 gtcaagggtc tccgccgcgc gccggccttg tcctgcagcg cccgccagcc cttgccgtcg
     3181 aagttctcca gcttctcgaa gccgaacgcg cggttgagcc cgggggcggc atcgagggcc
     3241 atcattgccg cctcgatgag aaaggtcccg ctgccgcaca tggggtcaaa caatggtgtc
     3301 ggtgccttcc aacccgtcag cttgagtatt cccgctgcga ggttttcctt taatggcgca
     3361 tcacccgttg tgatgcgcca tccgcgcttg aacagagctt cacccgaggt atcgagatag
     3421 aaagtggcct ggtcccgtgt caggaagacg tggatgcgca cgtcgggcct cgcagtgtcc
     3481 acgtccgggc ggctgccgcg cgcatcgcgg aacgcatcgc agacggcgtc cttgacgctc
     3541 agcgtgacga agtcgaggct cctgacgggg gacttgatcg ccgaaacgtt gacgcggatc
     3601 gtgcggcgca cgtcgaagaa ccgcggccat gccagcgcgc gcgccgcgct gaaaatctcc
     3661 tgctcggtcc ggtagccggc gcaccctacc tgccacagca cgcggctcgc cacgcggctc
     3721 tccaggttgg cggcatagca cagcgcaagg tcgccgccga aggcgacccc gccatcgacc
     3781 gggctgacat cgcgcgcgcc cagcgccgcc agctcgtccg cgagcacctg ctcgaggccc
     3841 cgcgggcatg tcgcgaataa acgctcggtc aagtgcgata gacgcgaggc gatagacgag
     3901 aggcggattg tccgtctatc gtctctcgcc cggcctctct ccggtcaggc cttggcacgg
     3961 cgcacgggat tgctcagcac cccgatgtcc ttctggat
//
LOCUS       DBNGSG010000034         1231 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3066337, whole genome shotgun sequence.
ACCESSION   DBNGSG010000034 DBNGSG010000000
VERSION     DBNGSG010000034.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3066337"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..788
                     /gene="maiA"
                     /locus_tag="AC158Q_00755"
     CDS             144..788
                     /gene="maiA"
                     /locus_tag="AC158Q_00755"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - L-tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HIR3338327.1"
                     /translation="MKLYGYFRSSASYRVRIALNLKGLSCEQASVHLSRGGGEQFSPA
                     FRRLNAQSLVPVLEDDGLALTQSLAIIEYLEEKHPKPALLPATPEARARVRSLALAIA
                     CEIHPLNNTRVLAYLTGPLGLGNDTRNTWYHHWIATGLQALETRLAAERETGKFCHGG
                     APGLADCCLVPQLANARRFKCDVAPYPTLLAIEKNCLALDAFQRAAPDRQPDAE"
     gene            834..>1231
                     /locus_tag="AC158Q_00760"
     CDS             834..>1231
                     /locus_tag="AC158Q_00760"
                     /inference="COORDINATES: protein motif:HMM:NF015692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HIR3338328.1"
                     /translation="MSGVSPREPHLKEHAIVGCVLGTAVGDALGLALEGLSRQRQPRM
                     FPDLSGYKLLPFGKGLCSDDTEHTCMLAQSLIGVAGLDTDLAARQFASAFGWRLRFWL
                     LGMPAGIGLATLRAIVKLWIGFPARHSGVYS"
BASE COUNT          218 a          403 c          369 g          241 t
ORIGIN      
        1 gacaggatta acaggattca caagattagt actcgcaaaa tccaagaaaa cagtctttgt
       61 ttgaatcctg ttaatcttgt taatcctgtc tattggtggt tttgacgggt tttgaatcat
      121 ttcgttaatc ctgtctattg cccgtgaagc tatacggata cttccgcagc tccgcctcct
      181 accgcgtgcg catcgcgctg aatctcaagg gcctctcctg cgagcaggcg tcggtgcatc
      241 tctcgcgcgg cggcggcgag cagttctcgc cggcgttccg ccggctgaac gcgcaatcgc
      301 tcgtgccggt gctggaggat gacgggctcg cgctgaccca gtcgctggcg atcatcgagt
      361 acctggaaga aaagcatccg aaacctgccc tgctccccgc gacgccggaa gcgcgggcgc
      421 gggtgcgctc gctcgcactg gcgatcgcct gcgaaatcca tccgctcaac aacacgcgcg
      481 tgctggctta cctgacgggc ccgctgggtc tcggcaacga cacccgcaat acgtggtacc
      541 accactggat cgctacgggc ttgcaggcgc tcgagacgcg tctcgccgcc gagcgcgaaa
      601 ccggaaagtt ctgccacggc ggcgcgcctg ggcttgccga ctgttgcctc gtcccccagc
      661 tggccaacgc gcggcgcttc aagtgcgacg tggcgccgta tcccactctg ctcgcgatcg
      721 agaagaactg cctggcgctg gatgcgttcc agcgcgccgc acccgaccgg caacccgacg
      781 ccgaatgaat tcgcggcttg tcaccgcgaa ttcataaacg ataattttat cccatgagtg
      841 gcgtcagtcc acgcgagccg cacctcaagg agcacgcaat tgtcgggtgc gtcctaggta
      901 cagcggtggg cgacgcgctg gggctcgcgc tggaaggctt gagccggcag cgacagccgc
      961 ggatgttccc cgacctctcc ggctacaagc tgctgccgtt cggcaagggc ctgtgctccg
     1021 acgacaccga gcacacctgc atgctcgccc aatcgctgat cggggtcgca gggctggaca
     1081 ccgaccttgc cgcgagacaa ttcgcgtccg ccttcggctg gcggctgcgc ttctggctgc
     1141 tgggaatgcc cgccggcatc ggcctcgcga cattgcgcgc aatcgtgaaa ctgtggatcg
     1201 gttttccagc acgtcacagc ggcgtttact c
//
LOCUS       DBNGSG010000035         5952 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1707953, whole genome shotgun sequence.
ACCESSION   DBNGSG010000035 DBNGSG010000000
VERSION     DBNGSG010000035.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5952)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5952)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5952
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1707953"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="AC158Q_00765"
     CDS             <1..516
                     /locus_tag="AC158Q_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3338329.1"
                     /translation="GWVQGAFTALNPIVQILRPISPIAWIPLAILWFGVGDVSPIFLI
                     FLSSVFPMVVQTITGVHTIERQYLRAAENFGVSRSRLFRQVVIPATLPQIIVGMRISL
                     GVAWLVVVAAEMIALRSGLGYLIIDSRNAGNRYDLVIAGMVIIGLTGLLLDGAMRSLE
                     RLKTVRWRYAR"
     gene            506..1240
                     /locus_tag="AC158Q_00770"
     CDS             506..1240
                     /locus_tag="AC158Q_00770"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3338330.1"
                     /translation="MPAKIAVRNITKSYATDKGPRPVIAGIDFTVDDGEFVALVGPSG
                     CGKTTLLHILAGFEHPDQGQIEIDGVARSRPSPRGIVISQQGSVFPWLTVQQNLMFGL
                     NGHADSRALADHYVDLVGLKSFEKAYPHELSGGMLKRVEVARALAVKPEILYMDEPLG
                     ALDALTGLRMRIELLRILAEERHTSLLVTHNVEEAIHMADRIMVLSTRPSTIQAAFDV
                     PFPHPRDVSRPEAQQLKVAILRELGL"
     gene            complement(1235..2269)
                     /gene="mutY"
                     /locus_tag="AC158Q_00775"
     CDS             complement(1235..2269)
                     /gene="mutY"
                     /locus_tag="AC158Q_00775"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HIR3338331.1"
                     /translation="MSGSFSAKLIAWQKKHGRHDLPWQGTRDPYAIWLSEVMLQQTQV
                     ATVIPYYNRFLARFPDVSALAAAPLDEVLRLWSGLGYYSRARNLHGAAQEIVAAHGGR
                     FPRRYEQVTALPGIGRSTAAAICIFAFGARHAILDGNVKRVLTRLRGIKGYPGEKKVA
                     DRLWQEAERLLPRRGVQAYTQGLMDLGATLCTRGKPRCADCPVHGGCIARRRGWVAAL
                     PASRPRKALPHKRTVMLALIRAGEVLLEKRPAAGIWGGMWCLPEMARKTDLEAYCLKR
                     FGAHVVEIEELATIAHGFTHFSLDIRPLRVKVSALVPQGAEPGVVWLPFEEARGAAIP
                     APVRRVLAQL"
     gene            complement(2811..4694)
                     /locus_tag="AC158Q_00780"
     CDS             complement(2811..4694)
                     /locus_tag="AC158Q_00780"
                     /inference="COORDINATES: protein motif:HMM:NF017021.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HIR3338332.1"
                     /translation="MKILKYALFALAGLVVLAGAVLAYVAATFDPNQYKPQIVQAVKE
                     KTGRTLKLEGDIALSFWPSIGAKIGKASLSERASDREFAAVETVHVSLKLLPLLSRQA
                     VADTVRVKGLRASIVKAKDGRTNVDDFTGPPAAKGAPAAKDARTDFGLDIAGVEIADA
                     AITYTDQGAGTKYQLSKLNFKTGRIAPGVPTAIEFSAHAQGDKPKLDLPVTLKTRLTL
                     DAAKQSLALEDLVLEAKGAAAGLANLSIRLTGNASVDNARQQVRAALAGKLADSSIKA
                     QVETAGFTAPAIRFDVDIDQIDVDRYLPQQAGSGKPAGAGKKPEKESPFDLTGLRSLR
                     ASGLLKVGALKANNVRATNVRMDVKASAGRLEVNPLTAVFYQGSLSSVVTVNAAPATP
                     TFAAKHNMNGINVGAFIKDLAGNDTLEGKGNLTADVTTQGSTVSALKKALGGNAAVKL
                     TDGAVKGIDIAGSIRNARAKLGALRGEQTQAADKSQRTDFSELTATFNIKNGVAHNND
                     LSLKSPLLRVGGEGDINVGEDTINYLVKASIVGTSKGQGGRDTDELKGVTVPVRVTGP
                     LAAPSYKLDFGAMATDTAKQKAMDELQKRLGGGAAEGDATKKDGAKSGDGTRDRLRGI
                     LGR"
     gene            4963..5931
                     /locus_tag="AC158Q_00785"
     CDS             4963..5931
                     /locus_tag="AC158Q_00785"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3338333.1"
                     /translation="MQHGKAIATLAGVIALTCLGTGTAAAQSFPDRPIRIIVPFAAGG
                     PNDILARAVGKNLSEKLGQQVIADNRPGGGTVIGTEIAARAPADGYTLLMASTSHTVN
                     PTLRRSLPFDTLRDFSHVILIAKAPNILVVHPSVPARSVRSLIALSRTKPGEVAYASG
                     GNGTSTHLTGALLSSMAKVKMIHVPYKGGAPAVTALISGEVSWMFGTFLASIPHIRSG
                     RMRVIATGGLKRSTVLPDAPTVAETLPGFESVGWYGVLAPAGTPQAVIAKLNAEINRG
                     LKDLQPILLRDGAETIGGTPGEFAAHFRAEMAKWAALLKEIGLQPS"
BASE COUNT          998 a         2002 c         1868 g         1084 t
ORIGIN      
        1 gggtgggtcc agggcgcgtt tacggcgctg aatccgatcg tgcagatact ccggccgatc
       61 tcacccatcg cctggatccc cctggccatt ctctggttcg gcgtggggga cgtctcgccg
      121 atctttctca ttttcctctc gtcggtgttt cccatggtcg tgcagaccat caccggcgtg
      181 cacaccatcg agcgccagta ccttcgcgcg gcggaaaatt tcggcgtgtc ccggtcccgg
      241 cttttccgcc aggtcgtgat ccccgcgacc ctgccccaga tcatcgtggg catgaggatc
      301 agcctcggcg tggcctggct ggtcgttgtc gcggcggaga tgatcgccct ccgatccggt
      361 ctcggctacc tgatcatcga ttcgcgcaac gcgggcaacc gctacgacct ggtgatcgcc
      421 ggcatggtca tcatcgggtt gaccgggctc ctgctggacg gcgcgatgcg gagcctggaa
      481 agactcaaga cggtcaggtg gcgttatgcc cgctaagatc gcggtcagga acataacaaa
      541 aagctacgcg accgacaaag gcccccggcc ggtcatcgcg ggcatcgatt tcacggtgga
      601 cgacggcgaa ttcgtggccc tggtcgggcc atccggctgc ggcaagacca ccctcctgca
      661 catcctggcg gggttcgagc accccgatca ggggcagatc gagatcgacg gcgtggcgag
      721 gagccggccc agccccaggg gtatcgtcat ctcccagcag ggttccgtct tcccctggct
      781 cacggtgcag cagaacctca tgttcggatt gaacggccac gccgacagcc gcgcgctcgc
      841 cgaccattac gtcgacctgg tgggcctgaa aagcttcgag aaggcctatc cgcacgagct
      901 gtcgggcggg atgctgaaaa gagtggaagt ggcgcgggca cttgccgtga agccggaaat
      961 cctctacatg gatgagccgc tcggggccct ggacgcgctc acgggactgc gcatgcgcat
     1021 cgagctgctg cgcatcctgg cggaggagcg ccataccagc ctgctggtca cgcacaacgt
     1081 ggaggaagcg atacacatgg ccgaccggat catggtcctc tccacccgtc catccacgat
     1141 ccaggcggcc ttcgacgtgc cgttcccgca tccgcgggac gtttcgaggc ccgaggcaca
     1201 gcagctgaaa gtggcgatcc tgcgcgaact cgggctatag ctgcgcgagg acgcgcctca
     1261 ccggcgcggg gatagcggcg ccgcgcgcct cctcgaacgg cagccacacc acgcccggct
     1321 ccgcgccttg cggcaccagc gccgacacct tcacgcgcag cggccggata tcgaggctga
     1381 agtgggtgaa gccatgcgca atcgtcgcga gctcctcgat ctcgacgaca tgcgcgccga
     1441 accgcttgag gcaataagcc tccaggtcgg ttttccgcgc catctccggc aggcaccaca
     1501 tgccgcccca gatgccggcc gcgggacgct tctccagcag gacctcaccc gcgcgaatca
     1561 gcgcgagcat gacggtgcgc ttgtgcggca gcgccttgcg cgggcgcgac gccggcagcg
     1621 cggcgaccca gccgcgccgg cgcgcgatgc agccgccatg caccgggcag tcggcgcagc
     1681 gcggcttgcc gcgcgtgcaa agggtggcgc ccagatccat caggccctgc gtgtaggcct
     1741 ggaccccgcg gcggggcaac agccgttccg cctcctgcca gagacggtcg gcaaccttct
     1801 tctcccccgg gtagcccttg atgccccgca ggcgcgtcag cacgcgcttg acgttgccgt
     1861 ccaggatggc gtgccgcgcg ccgaaagcga atatgcagat ggcggcggcg gtggaacggc
     1921 cgatccccgg cagcgccgtc acctgctcgt agcgccgcgg gaaacgcccg ccatgcgccg
     1981 ccacgatttc ctgtgccgcg ccgtgcaggt tgcgcgcgcg cgagtagtaa ccgagcccgc
     2041 tccataaccg cagcacctcg tcgagcggcg ccgccgcgag cgccgacaca tcggggaagc
     2101 gcgcgaggaa gcgattgtag taaggaatga cggtcgcgac ctgggtctgc tgcagcatga
     2161 cctccgagag ccagatggca taggggtcgc gcgtgccctg ccacggcagg tcgtggcggc
     2221 cgtgcttctt ctgccaggcg atcagcttcg ctgagaaact tccgctcacg tcaaaacctc
     2281 aaattagccg ccaagacgcc aagaacgcca aggaattcca aaatagaaga attaactagt
     2341 attaggtgac agtcgcctgg ttgggaaaag tatataccgg tagcgagaag tcactattag
     2401 tggtgataaa tccccggtaa tagcgaacca tcccgggcga taccggggcg ctctggaatt
     2461 ggagttatgt cccgagcgtt gaacggcgtt ccttcgtccg ttcgaacgcg aggagttaaa
     2521 ccggcgcgaa gcgtccggat ttaactagtc ttaggtaaca gtcatctggc tgggaaaagt
     2581 atataccggt ggcgagaaat cgctattagt ggtgacaatt ccccggcaat agcgaaccgt
     2641 cctgggcaat accgaggtac tctggaattg aaatcagatt ccgcgtgttg agcggcgatc
     2701 gtgtcgtccg atcaaacacg cggagttaaa ccggcgattt gggcacggga gccgcggctc
     2761 ccgcaccctg ggcgtccgga tttaacggta tttggcggtt agtttgaaac tcaacgcccg
     2821 aggatgcccc tcagcctgtc ccgcgtgccg tccccggact tcgcgccgtc tttcttcgtg
     2881 gcatcgccct ccgcggcgcc tccgccgagc cgtttctgca attcgtccat cgccttctgc
     2941 ttggctgtat cggtcgccat cgcgccgaaa tccagcttgt aggacggcgc cgcgagcggg
     3001 ccggtgacgc gcaccggcac ggtgacgccc ttcagttcgt cggtgtcgcg cccgccctgc
     3061 cccttggacg tgcccacgat ggaggccttg accaggtagt tgatcgtgtc ttccccgacg
     3121 ttgatgtcgc cctctccacc gacgcgcaac aacggcgatt tgagcgacag gtcgttgttg
     3181 tgcgcgaccc cgttcttgat gttgaacgtc gcggtgagct ccgaaaaatc ggtcctctgg
     3241 ctcttgtccg ccgcctgggt ctgctcgccg cgcagcgcgc cgagcttcgc cctggcattg
     3301 cggatcgaac cggcgatgtc gatgcccttc accgcgccgt cggtgagctt tacggcggcg
     3361 ttgccgccca acgccttctt gagcgcggag accgtgctcc cctgcgtggt cacgtccgcc
     3421 gtcaggttgc ccttgccctc gagcgtgtcg ttcccggcga ggtccttgat gaacgcgccc
     3481 acattgatgc cgttcatgtt gtgcttcgcg gcaaaggtcg gcgtggcggg cgccgcgttg
     3541 accgtgacca cgctcgacag cgaaccctgg tagaagaccg ccgtcaacgg gttcacttcc
     3601 agccggcccg cgctggcctt gacgtccatc cgcacgttgg ttgccctgac attgttggcc
     3661 ttgagcgctc cgaccttcag caagccggaa gcccgcaggc tcctcaggcc cgtcaggtcg
     3721 aagggagact ctttctccgg ttttttcccg gcgccggcgg gtttcccgct tcctgcctgc
     3781 tgcggcagat agcggtccac gtcgatctgg tcgatgtcca cgtcgaagcg tatcgccggc
     3841 gcggtgaaac cggcggtctc gacctgcgcc ttgatgctgc tgtccgcaag cttgcccgcc
     3901 agcgccgccc ggacctgctg cctggcattg tccacgctcg cgttgcccgt caggcggatc
     3961 gagaggttcg caaggccggc cgccgcgccc ttggcttcga gcaccaggtc ctccagcgcc
     4021 agcgactgct tcgccgcgtc cagcgtgagc cgcgtcttca gcgtgacggg cagatccagc
     4081 ttcggcttgt cgccctgcgc gtgcgcggaa aattcgatgg ccgtcgggac ccccggcgcg
     4141 attctcccgg tcttgaagtt gagcttcgac aactggtact tcgtccccgc tccctggtcc
     4201 gtgtaggtaa tcgcggcatc cgcgatttcc acgcccgcga tatccagccc gaaatccgtc
     4261 ctggcgtctt ttgccgcggg cgcccccttc gcggccggcg ggccggtgaa atcgtccacg
     4321 ttggtcctgc cgtccttcgc cttcacgata ctcgcgcgca gtcccttcac ccggacggta
     4381 tccgcgaccg cctgcctcga cagcagcggc aggagcttga gggagacgtg cacagtctcc
     4441 accgccgcga attccctgtc gctggcgcgc tccgacagcg aggccttccc gattttcgcg
     4501 ccaatgctgg gccagaacga aagcgcaatg tcgccctcga gcttgagcgt gcgcccggtt
     4561 ttctccttta ccgcctgcac gatttgcggc ttgtactggt tggggtcgaa tgtcgcggcg
     4621 acgtaggcca gcaccgctcc ggcgaggacg accaggccgg ccagggcaaa caatgcgtat
     4681 ttgaggattt tcatgggtcg ggctcccggt ttatccccgc gcattgagcg gcggatcaga
     4741 atacgaatca gattacttcg gccacgagta ccgcatcccg ggaccgcccg ttccaatgcg
     4801 cggagtcaaa ctggtgcaaa gcgtccagtt taacaacagt ttgaacacgc tagaattgcg
     4861 gggttccatc ggaaccggat ccggcgcttg agtccgtccg gaggaaacgg gtcccggcgc
     4921 cgcttctccg gcgttgaacc cataatatac gaggaggtaa ccatgcagca tggcaaagct
     4981 atcgcaacgc tggccggcgt cattgcgttg acatgcctgg gaaccggcac ggccgcggcc
     5041 cagagcttcc ccgacaggcc catccgcatt atcgtgccgt ttgccgcggg cggtcccaac
     5101 gacatcctgg cgcgcgccgt cggcaagaac ctctccgaga aactgggcca gcaggtcatc
     5161 gccgacaatc gtcccggcgg cggcacggtg atcggcaccg agatcgcggc gcgcgcgccc
     5221 gccgacggct acacgctgtt gatggcctcg accagccata ccgtgaatcc cacgctgcgc
     5281 cgctccctgc cgttcgacac gctcagggat ttttcgcacg tcatcctgat cgcgaaagcg
     5341 cccaatatcc tggtcgttca tccttccgtg ccggcccggt cggtcagatc cctgatcgca
     5401 ctgtcgcgga cgaagcccgg cgaggtggct tatgcttccg gcggaaacgg cacgtccacg
     5461 cacctgacgg gagcgctgct gagctcgatg gcgaaggtga agatgatcca tgtcccttac
     5521 aagggcggcg cgccggcggt caccgccctc atcagcggcg aggtctcgtg gatgttcgga
     5581 acgtttctcg cgtcgattcc ccacatccgt tccggcagga tgcgcgtcat cgcgaccggc
     5641 ggcctgaagc gctcgacggt cctccccgat gcgccgactg tcgcggaaac gctgcccgga
     5701 ttcgaatcgg tgggatggta cggagtcctc gctccggcgg gcacgccgca ggccgtgatc
     5761 gcgaagctca acgccgagat caaccgcggg ctcaaggacc tgcaaccgat cctgctgcgc
     5821 gacggcgccg agacgatagg cggaacgccg ggcgagtttg ccgcgcactt tcgcgccgaa
     5881 atggccaagt gggccgcgct gctgaaggaa atcggcctgc aaccctcgta gcaatcggcg
     5941 caccgcaccc gc
//
LOCUS       DBNGSG010000036         6865 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2557365, whole genome shotgun sequence.
ACCESSION   DBNGSG010000036 DBNGSG010000000
VERSION     DBNGSG010000036.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6865)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6865)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6865
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2557365"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1634)
                     /locus_tag="AC158Q_00790"
     CDS             complement(<1..1634)
                     /locus_tag="AC158Q_00790"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HIR3338334.1"
                     /translation="MAVDPERIRCIDPQVSPQYPFDEAPRTPERSEPRYAAFSDYAAC
                     IPMRDNVRLAADVNRPSARGMKFPALVTTSVYTRQLQRGVIALGQNEAGISEFWVPRG
                     YAHVIVDVRGSNDSEGHYDLFGAQEQQDLYDIIEWVAAQPWCDGNVGMSGVSYMGRSQ
                     LFAAEQQPPHLKAIFPYDASCDFYRDACFHGGIPTNFTTHWTQFVMNLHMTSGRNPNV
                     ESLKKQLELMYSHQYPFDGPFYRERSAGQRLDKVKIPSYFGCGWYMNELHLKGAFDGY
                     NGTGAIPKRMMVGPRPWPLRPWAGYHYEMLRWYDHWLKGMDTRVMEGAPIRLYIHGDE
                     RWREEKEWPLARTQWKEFYLGGPANGREGKLLDAAGPEQARRYEVDGLAAGANLGEPR
                     LIYRTEPMAKDMELTGPVALYLNASSTAPDVDWLVYLVDEAPDGKHRELCKGWLRSSH
                     RKIDPAKSTQAKPYHPHLEAEPLAPGTTTGFPIEIWPVCNVFKAGHRIRLEIANSDSM
                     VAANGRPHVWIRTRATNTIHEGGSEPSRLVVPVIPR"
     gene            complement(1712..2695)
                     /locus_tag="AC158Q_00795"
     CDS             complement(1712..2695)
                     /locus_tag="AC158Q_00795"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3338335.1"
                     /translation="MDRFLAKACSTLLILSMGGFAAGAAYAQAPAYPTRAIRMVIALA
                     PGGGVDTTGRFIAQKLNEAWGQPVVTDNRPGAGGTIAADLVARASPDGYTLLMTSAGI
                     TITPSLMKLSYDPLKDLLPVTMAVVSPGVLVVHPSLPVKNVKGLVAFAKARPGELFFS
                     SSGQGSAQHLTVELFSQMTGIKMTHVPYKGTAPSILDAVAGRVALTVASVISTRPMFT
                     TGKLRALAVVGPKRTPALPEYPTIAESGVPGFGVENWYALFLPGGTNREIAVKIQGVV
                     SRVLSEPGTTKLLRGQGLDAIGSSQDEFAKVYAGEISKWAKVVKTVGVKAN"
     gene            complement(2940..4166)
                     /locus_tag="AC158Q_00800"
     CDS             complement(2940..4166)
                     /locus_tag="AC158Q_00800"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3338336.1"
                     /translation="MKIFYGWRIVVAGGALQFLQSLLLNQSFGVYLAVLVEDRGWSKT
                     AVAGAAALKSTEVAVLGPVLGWAVDRYGAQGIVRAGIVTFGIGFMLLSQIETLAGFYA
                     AFVVVALGSSMFSNSIVGVAIIQWFEKRRARALSALQFGSAVGGLFVFLVAASIQAFG
                     WRATAFSSGVIAILIGWPLVRVIRSRPEDIGETVDGLPPASKGDTPHAAAKPVRHGLT
                     AREALRTSAFWLIALGHACALLVVSAVNVHAVAHIKEGLGYSLALATLAFTLVTIGQF
                     FGVVAGWVIGDRFQKRLVAAACMLMHAAGLLMLTYATGPVVLAIAALVHGTAWGLRGP
                     FMQAIRADYFGRSSIGMIIGLSSLIIVIGNIAGPILAGAFADWTGNYRMGFTILAVLS
                     GLGSLFFLMAKPPKQT"
     gene            4406..5329
                     /locus_tag="AC158Q_00805"
     CDS             4406..5329
                     /locus_tag="AC158Q_00805"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIR3338337.1"
                     /translation="MKAAFFMKNGGPEVMQYGDVPDPAVKPGEVLVDVHAASVNGADW
                     KVRAGHYAPITTFPYIPGRDFSGVVSALGAGVTDLKAGDAVFGVVEQTSEACYAEKVA
                     IKAAIVAKKPDSLSHVEAASLALIGLTALVSIEDTLKLQSGETILIQGGAGGVAGFGV
                     QVAKHIGARVITTASAANHDYLRSLGADQIIDYRKQDFTKAVSGVDAVFDTVGGEVVK
                     GSFAVLRPGGRAAFIGSGPTAPASPRSDVVSLRPKVGRDRAHLERVVALVAAGAVRVP
                     EIKIYSLSQAAEAHRVSEGRHLRGKLVFKLR"
     gene            complement(5576..6082)
                     /locus_tag="AC158Q_00810"
     CDS             complement(5576..6082)
                     /locus_tag="AC158Q_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HIR3338338.1"
                     /translation="MAVSMYPISVPVFIKHLNGLAGCLKKAQALYAEKKFDEASLLHY
                     RFYPDMFTFARQVQAMADHAKNCTAMLAGMEAPKYEDNEKSLAELIARIEKTIEFLKT
                     VKPAQIDGTEEKPITIKMRDRELNMKGVELVLNRSLPNFYFHATTAYDILRHNGVELG
                     KRDFMGNA"
     gene            complement(6209..6745)
                     /locus_tag="AC158Q_00815"
     CDS             complement(6209..6745)
                     /locus_tag="AC158Q_00815"
                     /inference="COORDINATES: protein motif:HMM:NF014664.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3338339.1"
                     /translation="MTFVQPLRPEEIRDPELRELIRRCEELGVPDARFPRILARAPGY
                     AKALLRALLHSHTEGAVDHRLKEIIRVRLARTAGDPYFASLRSARARGEGLDESRIEA
                     GAGPFESDARFTAAEKWALRYAREMYLNPEKVDAAFYAEGKTHYTEAQIMELGAFIAF
                     HYGMQAFARTLGAGLGNG"
BASE COUNT         1188 a         2214 c         2254 g         1209 t
ORIGIN      
        1 tatctgggta taaccggcac taccaatcgc gatggctcgg agccaccttc atggatggtg
       61 ttggtggccc tggtcctgat ccacacgtgc ggccggccat tggcggcgac catggagtcg
      121 gagttcgcga tctcgagccg gatgcggtgc ccggccttga acacgttgca caccggccag
      181 atctcgatcg ggaacccggt tgtcgttccg ggcgcgagcg gttcggcctc gaggtgcggg
      241 tggtacggct tcgcctgcgt ggatttcgcc gggtcgatct tcctgtgcga cgaccgtagc
      301 cagcccttgc acagctcgcg gtgcttgccg tcgggtgctt cgtccaccag gtacaccagc
      361 cagtcgacgt ccggcgcggt ggatgaggcg ttgaggtaga gcgccaccgg accggtcaac
      421 tccatgtcct tcgccatcgg ctcggtgcgg taaatcagcc gcggctcgcc caggttcgcc
      481 ccggcggcga gcccgtcgac ctcgtagcgc cgcgcctgtt ccggccccgc cgcgtcgagc
      541 agcttgcctt cgcggccatt cgccgggccg ccgaggtaga actccttcca ctgcgtgcgc
      601 gcgagcggcc actccttctc ctcgcgccag cgctcgtcgc cgtggatgta gagccggatc
      661 ggcgcgcctt ccatcactct cgtgtccatg cccttcagcc agtggtcgta ccagcgcagc
      721 atctcgtagt ggtagcccgc ccacggccgt agcggccacg ggcgcggccc caccatcatg
      781 cgcttcggga tcgcgccggt gccgttgtag ccgtcgaacg cgcccttcaa gtgcagctcg
      841 ttcatgtacc agccgcagcc gaagtaggac gggatcttca ccttgtcgag ccgctgcccg
      901 gccgagcgct cccggtagaa cgggccgtcg aacgggtact ggtggctgta catgagttcg
      961 agctgcttct tcaggctctc cacgttcgga ttgcgccccg aggtcatgtg caggttcatg
     1021 acgaactggg tccagtgcgt cgtgaagttg gtcgggatgc cgccgtgaaa gcaggcgtcg
     1081 cggtagaaat cgcacgaggc atcgtagggg aagatcgcct tgaggtgcgg cggctgctgc
     1141 tcggcggcaa agagctggct gcggcccatg taggagacgc cgctcatgcc gacgttgccg
     1201 tcgcaccacg gctgcgcggc gacccattcg atgatgtcgt acaggtcctg ctgctcctgg
     1261 gcgccgaaga ggtcgtagtg gccttccgaa tcgttgctgc cgcgcacgtc cacgatgacg
     1321 tgggcgtagc cgcgcggcac ccagaattcg gaaatgccgg cctcgttctg gccgagcgcg
     1381 atcacgccgc gctggagctg gcgcgtgtag acggaggtcg tcaccagcgc cgggaacttc
     1441 atgcctcgcg ccgagggccg gttcacgtcg gcggcgagcc ggacgttgtc gcgcatcggg
     1501 atacacgcag cgtagtcgga aaaggccgca tagcgcggct cggagcgctc cggcgtccgc
     1561 ggcgcctcgt cgaacgggta ctgcggcgag acctgtggat caatgcagcg aatgcgctcg
     1621 ggatcgacgg ccatggtgaa tggcgtctca ggggggctgt cccccgagac gctggttttt
     1681 atacgatagg cagcgggatt ttccgctcgt tctagttcgc cttcaccccg acggtcttca
     1741 cgacctttgc ccatttcgag atctcgccgg catagacttt ggcgaactcg tcctgcgagc
     1801 ttccgatggc gtcgagaccc tggccgcgca gcagcttggt cgtgcccggt tcggacagca
     1861 cgcgggatac gacgccctgt atcttgaccg cgatttcccg gttggtgccg cccgggagaa
     1921 acaacgcgta ccagttttcg actccgaatc cgggcacgcc cgattcggcg atcgtggggt
     1981 actcgggcag tgccggggtg cgcttggggc ccaccacggc cagcgcgcgc agcttgcccg
     2041 tcgtgaacat cggccgggtc gagatcacgc tcgcgacggt gagggcgacc cgcccggcga
     2101 ctgcgtcgag gatgctcggc gccgtgccct tgtacggcac atgggtcatc ttgatcccgg
     2161 tcatttgcga gaacagctcg acggtcaaat gctgcgccga gccctggccc gaggacgaga
     2221 agaacagctc gcccggcctc gccttggcga aggcgaccag ccccttcacg ttcttcacgg
     2281 gcagggacgg atgcaccacc agcacgccgg gcgaaaccac cgccatcgtc acgggcagca
     2341 ggtccttgag gggatcgtag gacaatttca tgaggctcgg cgtgatggtg attcccgccg
     2401 aggtcattag cagggtatag ccatcggggg acgcgcgcgc caccagatcc gcggcgatcg
     2461 ttccaccggc gccggggcga ttgtcggtca ccaccggctg gccccacgct tcgttcagtt
     2521 tctgggcaat gaaccggccg gtcgtatcga cgcccccgcc cggcgccagc gcgatgacca
     2581 tccgtatcgc ccgggtggga taggcgggcg cttgcgcgta agcggcgccc gccgcgaaac
     2641 cgcccatcga cagaatcagc agggtgctgc aagcctttgc gagaaatcga tccatcgtct
     2701 cctccattat gatgttatgt tgttgtccgg cgtctcaatg cgaagtatac aagcggtcct
     2761 taactagaca accttgcaaa ccggtgaggg atgacgggca agcggttcca cgcaactaac
     2821 ccgcctgctt cgcatccacc cctccttgag aaggagggga gtaaagcggt aacggtcatt
     2881 ttgctaccgg ttctcaggtt ttcgatatcg gtttcctttg tgtcctctgt gtcaaagctt
     2941 tacgtctgct tcggcggttt ggccatcagg aaaaacaacg accccagtcc cgacagcacc
     3001 gccaggatcg taaaacccat ccggtagttc ccggtccagt ccgcgaacgc gccggccagg
     3061 atcggcccgg caatattgcc gatcacgatg atcagggacg acagcccgat gatcatgccg
     3121 atcgagctgc ggccgaagta gtctgcgcgg atcgcctgca tgaagggccc tcgcaggccc
     3181 caggcggtgc catgcaccag cgccgcgatg gccagcacga cgggaccggt ggcgtaggtg
     3241 agcatgagca ggcccgcggc gtgcatcagc atgcaggctg ccgcgaccag gcgcttctgg
     3301 aaacggtcgc cgatcaccca acctgcgacc acgccgaaga actggccgat cgtcaccagg
     3361 gtgaacgcaa gcgtggcgag ggcgagcgaa tagcccaggc cttccttgat gtgggcgacg
     3421 gcatgcacgt tcacggcgga taccaccagc agcgcgcacg cgtggccgag cgcgatcagc
     3481 cagaacgcgc tggtgcgcag cgcctcgcgc gcggtcaagc cgtgccgcac cggctttgcc
     3541 gccgcatgcg gagtatcgcc cttcgacgcg ggcggaaggc cgtccacggt ttcgccgata
     3601 tcctccgggc ggctgcggat cactcgcacc agcggccagc cgatcaggat cgcgatgacc
     3661 cccgagctga aggcggtcgc ccgccagccg aacgcctgga tcgaagcggc gacgaggaac
     3721 acgaacagcc cgccgaccgc gctgccgaac tgcagcgcgg agagcgcgcg ggcgcggcgc
     3781 ttctcgaacc actggatgat cgcgacgccc acgatggagt tgctgaacat gctcgagccc
     3841 agcgcaacga ccacgaacgc cgcgtagaag ccggcgagcg tttcgatctg gctcaagagc
     3901 atgaagccga tgccgaacgt gacgatgccg gcgcggacga tgccctgcgc cccgtagcgg
     3961 tccaccgccc agccgagcac cgggccgagg accgccacct ccgtggattt cagcgcggct
     4021 gcgccggcga cggcggtctt gctccagccg cgatcctcga ccaggaccgc gagatagacg
     4081 ccgaaggact ggttcagcag caggctctgc aggaactgca gcgcgccgcc ggcgaccacg
     4141 atgcgccagc cgtagaagat tttcattgaa gcccccgagg atagcagaag gcggaagtcg
     4201 caaatactgc ggctcaggta aggcgtcgca gataaagcgt ctcaggtaag gcgtctcagg
     4261 tactgcgtct ccgatagagc gtctcaggta aggcgtcgca gaaaaagcgt cttaagtcaa
     4321 tgacgcctct tccgggacgc ttgatctggg acgattcatc tgcgacgctt cgcctgcgac
     4381 gttcaaacca cgaccgggag aggcaatgaa agcagcgttc ttcatgaaaa acggcgggcc
     4441 ggaggtgatg cagtacggcg atgttcccga tcctgccgtc aagcccggcg aagttctggt
     4501 ggacgtccat gcggcgagcg tgaacggggc cgactggaag gtgcgcgcgg gccactatgc
     4561 cccgatcacg accttcccct acattccggg gcgggatttc tccggcgtgg tgagcgcgct
     4621 cggcgccggc gtcaccgacc tcaaggccgg ggacgcggtg ttcggggtgg tcgagcagac
     4681 cagtgaagcc tgctacgcgg agaaagtcgc gatcaaggcg gcgatcgtgg cgaagaagcc
     4741 ggacagcctc tcgcacgtcg aagccgcctc gctggcgctg atcggcctga ccgcgctcgt
     4801 atccatcgag gacacgctga aattgcagtc cggcgagacg atactcatcc agggcggcgc
     4861 cggcggcgtg gccggcttcg gggtgcaggt cgccaagcac atcggggcgc gcgtcatcac
     4921 gaccgcgagc gcggccaacc acgattacct gcgcagcctc ggcgccgacc agatcatcga
     4981 ttaccgcaag caggacttca cgaaggcggt gtcgggcgtg gacgcggtgt tcgacaccgt
     5041 cggcggcgag gtggtcaagg gttcattcgc cgtgctgcgg ccgggcggac gcgctgcgtt
     5101 catcggctcc gggcccacgg cgcccgcttc gccgcgcagc gacgtcgtgt cactgcgccc
     5161 caaggtcggg cgcgaccgcg cccatctcga gcgcgtcgtc gcgctcgtcg ccgcgggcgc
     5221 tgttcgcgtg ccggagatca agatctattc gctgtcgcag gccgccgaag cccaccgcgt
     5281 gagcgaaggg cgtcacctgc gcggcaagct ggtgttcaag cttcgctgaa cgacaaggcg
     5341 aaagtcgaaa ggcgaaagtg tgaaaccgcg cgtaccgcgc gggctttgat caagaacccg
     5401 gcctaacaga ctgttgaaaa acgccgtcac tcccgcgaac gcgggagtcc agggaatcca
     5461 cctgtatatg gatcgacgag tgactggatt tccgccttcg cgggaatgac gttatcgttc
     5521 tgtgcgacac gtttttcaac actctcctaa cagtcgttac ttccggcttt cgccttcagg
     5581 cgttccccat aaaatcccgc ttgccgagct ccacgccgtt gtggcgcagg atgtcgtagg
     5641 cggtggtggc gtgaaaatag aagttgggca gcgagcggtt gagcaccagt tcgaccccct
     5701 tcatgttgag ctcgcggtcg cgcatcttga tggtgatggg tttctcctcg gtgccgtcga
     5761 tctgcgcggg cttcaccgtc ttcaaaaatt cgatcgtctt ctcgatgcgg gcgatcagct
     5821 cggcgaggct cttctcgttg tcctcgtatt tcggcgcttc catcccggcc agcatggcgg
     5881 tgcagttctt cgcgtgatcg gccatggcct ggacctggcg cgcgaaggtg aacatgtcgg
     5941 gatagaagcg gtagtgcagc agcgaggcct cgtcgaattt cttctcggcg tagagcgcct
     6001 gcgctttctt caggcagccg gcgaggccgt tcaagtgctt gatgaatacg ggcacggaaa
     6061 tggggtacat cgagacagcc atgggtggtc tccgaagggt tggtcagggg ggctattcta
     6121 actaaaggca caaggtacaa ggcacaaggc gcaaggcaca aggcacaagg tacaaggtac
     6181 aaggcgaaag ggtctatcta atcacccgtc acccattccc cagcccggcg cccagcgtgc
     6241 gcgcgaacgc ctgcatgccg tagtggaaag cgatgaaggc gccgagctcc atgatctgcg
     6301 cctcggtgta gtgcgtcttg ccctcggcgt agaaggcggc atccaccttc tcggggttca
     6361 gatacatctc gcgcgcgtat cgcagggccc atttctcggc ggcggtgaag cgcgcgtcgc
     6421 tctcgaatgg gcccgcgccc gcttcaatcc ggctctcatc caggccttcc ccacgcgcgc
     6481 gcgccgagcg caggctggcg aagtacgggt cgcccgcggt ccgagcgagc cgcacgcgga
     6541 tgatttcctt gagccggtgg tccacggcgc cctcggtgtg cgagtggagc agggcgcgca
     6601 gcagcgcctt cgcgtacccc ggcgcgcgcg cgaggatgcg gggaaagcgg gcgtcgggca
     6661 cgcccagctc ctcgcagcgc cggatcagct cccgcagttc cgggtcgcgg atctcttcgg
     6721 gcctcagcgg ctggacgaac gtcatcgccc gcctcctatt cccttttcac tttccccttt
     6781 tcccttttca agggacacgg gcgctccgcc gtagatgcgc gcggactcct cctggctgac
     6841 gaggcgaccg tcgggcgaaa acatc
//
LOCUS       DBNGSG010000037         4405 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2727376, whole genome shotgun sequence.
ACCESSION   DBNGSG010000037 DBNGSG010000000
VERSION     DBNGSG010000037.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4405)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2727376"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..1872)
                     /locus_tag="AC158Q_00820"
     CDS             complement(103..1872)
                     /locus_tag="AC158Q_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HIR3338340.1"
                     /translation="MSGPPKKPQEASHSNFIRTHIDADLASGKYAARTWGGKPGPAKT
                     HEVAPLDPARIRTRFPPEPNGYLHIGHAKSICLNFGLARDYSGACHLRFDDTNPEKEE
                     QEYVDSIIAAVEWLGFSWENAGGSHLYYASDYFDFMYHAAEHLIETGHAYVDSLSAEQ
                     MRALRGTLNETGKDSPYRDRGVEESLKLFRGMRDGRFKEGEHVLRAKIDMASPNINLR
                     DPAIYRIRKAAHHRSGDKWCVYPMYTYAHPIEDALENITHSLCTLEFEDQRPFYDWLL
                     ERLARDGLIQRPPPQQIEFARLNLTYVVLSKRRLIQLVMDGFVNGWDDPRMPTLAGAR
                     RRGYTPEGIRLFAERIGVSKADSWIEYAVLEDCMREHLNEVAPRRMAVLDPVKLVIEN
                     YAEGREELCEVPNHPQKPDWGRRSVPFSRELWIEREDFMETPSKGYFRLFPGNKVRLR
                     YGYVVECIGYERNNEGGLTVRCTYDPDTKSGTPGAEKVKVKGNIHWVSVKHACAAEVR
                     LYDRLFKAPHPGKERDFLEDINPDSRKPVTAQLEPALKDAKAEERFQFERHGYFVADL
                     KDTRPGVPVFNRAVTLRDSWSKA"
     gene            complement(1950..2909)
                     /locus_tag="AC158Q_00825"
     CDS             complement(1950..2909)
                     /locus_tag="AC158Q_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3338341.1"
                     /translation="MGNASRGLFAFATVLLCTGVFAQSYPARPIRIIVPFPPGGTSDI
                     LARLIGPKLTEKWGQSVVVESRPGAAGAIGHELVAKSPPDGYTLVLSDVGPMVIVPVL
                     YPKTPYGLQDFAPITIISYSPHLLCVHPTVPVSTVPQLIALAKKRPGGLNYASSPAGA
                     PYLAGLQFAHRTGIKWEYITGRGGAQSIMDVMIGQADALFNGMLATLPPVKSGKLKLI
                     AVTSEKRHPSLPDTPAVAEFLPGFLTGSWQAVYAPARTPPDVVAALNAEVARVLFTPD
                     MKGRLQAQGADPIGTPSAESAKFLRDELGRFTKLVKETGYKAQ"
     gene            complement(3011..3745)
                     /locus_tag="AC158Q_00830"
     CDS             complement(3011..3745)
                     /locus_tag="AC158Q_00830"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF015534.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HIR3338342.1"
                     /translation="MRSVTPVASRVRKQIIAMGGAALPAELDNLLLVRYFLGQTGRKH
                     PKVCFIGAAGGDSENHRLRFYAGFGRFGCRTSHLPLFARTPCDLESFVLEHDAIFVGG
                     GNTRSMLAVWRDWGLDGHLRTAWERGVVLGGVSAGAICWFEQGLTDSVAGPLTAMDGL
                     GWLPGSCCPHYDSERQRRPAFRRLVAGGSMADGVAADDHAALHYVDATLLRAVSGAPR
                     ARAWSVRKSGNRAIEKALPVKELRAR"
     gene            complement(3742..4107)
                     /locus_tag="AC158Q_00835"
     CDS             complement(3742..4107)
                     /locus_tag="AC158Q_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3338343.1"
                     /translation="MKKILVIEDEADLRANVARFLKAEGYEVLLAENGAAGAEAALAQ
                     MPDLIVCDIAMPEMDGFGVLFTLRENVTTSHIPFIFLTASTRTYDRKWGVELGASDYI
                     TKPFKLEQLLVAIQTRLKG"
BASE COUNT          691 a         1422 c         1462 g          830 t
ORIGIN      
        1 tgagtgacgg gtgacgggtc aaatcacgac tcacgaaccc ccaccctggc cctccccctt
       61 ggcaaggggg agggttgggg agggggtcac gattcacgcc tttcaagcct tgctccagga
      121 atcccgcagc gtcaccgccc ggttgaacac gggcacgccc ggccgcgtgt ccttgaggtc
      181 ggccacgaag tagccgtggc gctcgaactg gaagcgctct tccgcttttg cgtccttaag
      241 cgccggttcg agctgcgccg ttaccggttt cctcgaatcc ggattgatgt cttcgagaaa
      301 gtccctttct tttcccggat gcggggcctt gaacagcctg tcgtagaggc gcacctcggc
      361 ggcgcaggcg tgcttcacgc tcacccagtg gatgttgccc ttcactttca ccttttccgc
      421 gcccggcgtg cccgatttgg tgtcggggtc gtaggtgcag cgcaccgtga ggccgccctc
      481 attattcctt tcgtagccga tgcactcaac cacgtagccg tagcgcaggc gcaccttgtt
      541 gcccggaaag aggcggaagt agcccttgga cggcgtttcc atgaaatcct cgcgctcgat
      601 ccacaactcg cgcgagaacg gcacgcttct cctgccccag tccggctttt gcggatggtt
      661 gggcacttcg cacagctcct cgcggccttc cgcgtagttc tcgatcacga gcttcaccgg
      721 gtcgagcacc gccatgcggc gcggcgccac ttcgttcaaa tgctcgcgca tgcagtcttc
      781 cagaacggcg tactcgatcc acgaatccgc cttcgacaca ccgatgcgct cggcgaagag
      841 gcggatgccc tcgggcgtat agccacggcg ccgcgcgccg gcgagcgtgg gcatgcgcgg
      901 gtcgtcccag ccattcacga agccatccat gaccagttgt atcaatcttc tcttggatag
      961 aacgacatac gtgaggttga ggcgcgcgaa ctcgatctgc tgcggcggcg ggcgctggat
     1021 caggccgtcc cgggccaggc gctcgagcag ccagtcgtag aacgggcgct ggtcctcgaa
     1081 ttccagcgtg cacagcgagt gcgtgatgtt ctccagcgcg tcctcgatcg ggtgcgcgta
     1141 ggtgtacatg ggatagacgc accacttgtc gccgctgcgg tgatgcgcgg ccttgcggat
     1201 gcggtagatc gccgggtcgc gcagattgat attgggcgag gccatgtcga tcttcgcgcg
     1261 cagcacgtgc tcgccttcct tgaaccgccc gtcgcgcatt ccgcggaaca gcttcaggct
     1321 ctcctcgacg ccgcggtcgc ggtaggggct gtccttgccg gtctcgttga gcgtgccgcg
     1381 cagcgcgcgc atctgttcgg cggagaggct gtccacgtag gcgtggccgg tctcgatgag
     1441 gtgctccgcc gcgtggtaca tgaagtcgaa atagtcgctg gcgtagtaca aatgcgagcc
     1501 ccccgcattt tcccagctga aacccagcca ttcgaccgcg gcaatgatgg aatcgacgta
     1561 ttcctgctct tccttctccg ggttggtgtc gtcgaagcgc aggtggcagg cgccgctgta
     1621 atcgcgcgcg agcccgaaat tgaggcagat ggacttcgcg tggccgatgt gaagatagcc
     1681 gttcggctcc ggcgggaagc gcgtgcggat cctggccgga tccagcggcg ccacttcgtg
     1741 tgttttcgcc gggcccggct tcccgcccca ggtgcgcgcg gcatatttcc cgctcgcgag
     1801 gtcggcgtcg atgtgggtgc ggatgaagtt cgagtgcgag gcttcctgcg gtttcttggg
     1861 cgggccggac atgccgctat tttagccgag tgtcctcggg atcagtgatg aagcgacgag
     1921 ccgcgtcact ccatcactgc atcatctcgt cactgcgcct tgtaccccgt ctccttcacc
     1981 agtttcgtga atcggcccag ctcgtcgcgc aggaatttcg cggattcggc ggacggcgtg
     2041 ccgatcgggt cggcaccctg cgcttgcagg cgtcccttca tgtcgggcgt gaacagcacg
     2101 cgcgccactt cggcattcag tgcggcgacg acgtccggcg gcgtgcgcgc cggggcgtac
     2161 acggcttgcc acgagcccgt caggaagccg ggcaggaatt cggcgacggc cggcgtgtcg
     2221 ggcaggctgg ggtggcgctt ttcgctagtg accgcgatca gcttgagctt gcccgacttg
     2281 accggcggca gcgtggcgag catgccgttg aacagcgcgt cggcctgccc gatcatcacg
     2341 tccatgatcg attgcgcgcc gccgcgcccg gtgatgtatt cccacttgat gccggtccgg
     2401 tgcgcgaact ggagccctgc gaggtacggc gcgccggcgg gactggaggc gtagttgagc
     2461 ccgcccgggc gtttcttggc gagcgcgatc agctgcggca ccgtggatac cggcacggtc
     2521 gggtgcacgc acagcaggtg cggcgagtag gagatgatgg tgatcggggc gaaatcctgc
     2581 aagccgtagg gcgtcttcgg gtagaggacg gggacgatta ccatcggacc gacatcgctg
     2641 agaaccaggg tgtagccgtc gggcggggac ttcgcaacca gctcgtggcc gatcgcgccc
     2701 gccgcgcccg ggcggctctc gaccaccacc gattgtcccc atttttcggt gagcttgggg
     2761 ccgatcagcc gcgccaggat gtcggaggtg ccgccgggtg gaaacggcac gatgatgcgg
     2821 atcggcctgg ccggatagct ttgagcgaag acgcccgtgc acagcagcac ggttgcgaaa
     2881 gcgaacaacc cgcgtgaagc gtttcccatg ttgcctccat tgtccgaaac cacacctgaa
     2941 gggaggcttc ctcgtgctcc cttggcaccg gaggccgttt cgctcgtgcc gaaacagccc
     3001 gccggaggac tcaacgcgcg cgcagctctt ttaccggcag cgctttctcg attgcgcgat
     3061 tgcctgattt tctcacgctc caggcccggg cgcgcggcgc gccgctcacc gcccgcaaca
     3121 gggtcgcgtc gacgtagtgc agcgccgcat ggtcgtccgc cgccacgcca tccgccatcg
     3181 agccgccggc aaccagccgc cggaaagccg gccggcgctg ccgctcgctg tcgtagtggg
     3241 ggcagcagct gcccggcagc cagcccaggc cgtccatcgc cgtgagcggg ccggcgacgg
     3301 agtcggtcag gccttgctcg aaccagcaga tcgcgccggc gctgacgccc cccagcacga
     3361 cgccgcgctc ccatgcggtg cgcaggtgcc cgtcgagccc ccagtcgcgc cacacggcga
     3421 gcatgctgcg ggtattgccg ccgccgacga agatggcatc gtgctcgagc acgaaggatt
     3481 cgaggtcgca cggcgtgcgc gcgaacagcg gcaggtgcga cgtcctgcag ccgaagcggc
     3541 cgaagccggc gtagaagcgc aggcggtggt tctcggagtc gccgccggcg gcgccgatga
     3601 aacagacttt cgggtgcttc ctgccggtct gcccgaggaa atagcgcacc aggagcaggt
     3661 tatcgagctc ggcgggaagg gcagcgccgc ccatcgcaat gatttgcttc ctgacgcggg
     3721 acgccaccgg cgtgaccgat ctcaaccctt gagccgtgtc tgaatcgcga cgagcaactg
     3781 ttcgagcttg aacggcttgg tgatgtagtc gctggcgccg agctccaccc cccatttccg
     3841 gtcataggtg cgggtgctgg cggtgaggaa gatgaagggg atgtgtgacg tggtgacgtt
     3901 ctcgcgcagc gtaaacagca ccccgaagcc gtccatttcc ggcatggcga tgtcgcacac
     3961 gatgagatcc ggcatctgcg cgagcgcggc ctccgcgccg gccgcgccgt tttccgcgag
     4021 cagcacctcg tagccctcgg ccttcaggaa gcgcgcgacg ttggcgcgga ggtcggcctc
     4081 gtcttcgatc accagtattt tcttcatgcg atattttacc taacagtgaa gtcgcttcct
     4141 cacgcgtctg cgcagagtgt tgagtgtcga gggtcgagtg tcgagggtcg agtgtcgggt
     4201 gtcgagtgtc gggtgtcgag tgacgggtgt cgagtgaaga tggatggccg tttaccccct
     4261 agtcactcca ttctcgttcc tcgcttcccc ctcgacactc aatcctcgac actcgcccct
     4321 tggctcccgg ccggaagccg gcggcggatc gccgccagca ggtcggcgat cctgaacggc
     4381 ttggtaacgt aatcgttggc gccac
//
LOCUS       DBNGSG010000038         5555 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_11676, whole genome shotgun sequence.
ACCESSION   DBNGSG010000038 DBNGSG010000000
VERSION     DBNGSG010000038.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5555)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5555)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5555
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_11676"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="AC158Q_00840"
     CDS             complement(<1..254)
                     /locus_tag="AC158Q_00840"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HIR3338344.1"
                     /translation="MSEATKGRPLAGKGIVVTRPTQQAQALARLIEGAGGRPVLFPVI
                     EIRDVEDLRPFTQLIDRLDEFDYAIFISPNSVERSMKLINA"
     gene            complement(320..1285)
                     /gene="hemC"
                     /locus_tag="AC158Q_00845"
     CDS             complement(320..1285)
                     /gene="hemC"
                     /locus_tag="AC158Q_00845"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008351000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HIR3338345.1"
                     /translation="MTAAGSTESAPGRIVIASRESALALWQARHIQSQLAALYPGAGV
                     EILGMTTEGDRRLDASLANMGGKGLFVKELEDALADGRADIAVHSVKDVPMELPRGFV
                     LAAIGERADPRDAFVSSRYAGLDALPAGARVGTSSLRRECQLRARHPRLRIEPLRGNV
                     PTRLKKLDDGNYEAVIVAAAGLKRLGLEDRITLLLAPSESLPAPGQGALGIECLADRT
                     DLIGQLAPLAHAATARCVEAERSFSRALSGNCNLPLGAYAEMEGKRIRLRGFVGAPDG
                     SRMVNGEQDGPADEPEALGAALAARLKAQGATGILAELERQSAAR"
     gene            complement(1362..1913)
                     /locus_tag="AC158Q_00850"
     CDS             complement(1362..1913)
                     /locus_tag="AC158Q_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338346.1"
                     /translation="MSFLIFAAAHGADQPYGLDDIALGASYARLAAALDFRYIHSAIA
                     AQKETKSAKPDLGRRGYGCARREDPFADIACVSHDERVDGVETREVRLHFLDGILQQF
                     SITAEIRQYEAVMQALRERHGAPQRMEPAPAGGYPASSWRNADSSIIAYGGKDLVFVS
                     FELAGYGAAVKRKQSGHQPQVCR"
     gene            complement(1975..2733)
                     /locus_tag="AC158Q_00855"
     CDS             complement(1975..2733)
                     /locus_tag="AC158Q_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIR3338347.1"
                     /translation="MNDERSLRVMIVDDEAPARLRLRDLLGECGGEPPLIVAGEAASG
                     PAALDLLSRSAVDVVLLDIRMPEMDGIEVALHLQQLERPPSVVFATAYDDYAIRAFEV
                     HAVDYLLKPIRLERLREALARARAAPPRPQALRELARAPRTHLCAQERGRIHLIPIAD
                     VIYLKAELKYVSVRTTTREYLIEESLTRLEQEFGDRFVRVHRNCLVARAAIRGFERSE
                     EGGETHWEVVLAGADERIAVSRRQQYIVREFGKA"
     gene            complement(2730..3749)
                     /locus_tag="AC158Q_00860"
     CDS             complement(2730..3749)
                     /locus_tag="AC158Q_00860"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF018305.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3338348.1"
                     /translation="MARSINQNAYTGGLPNFRNLGILLRIGVLVNVFAFAVAIVKSHD
                     LGALWQQTIQVASLVEPLLILSLLVLAALNGGLRRMPYWLGAAAVLVLELALTTGLYV
                     AGRTLLGPDPAPLERYWLLVALTTGALLVYFDARGRALSPALTEARLQALQSRIRPHF
                     LFNSINAVLSLIRQEPRRAEAALEDMAGLFRMLMEDNRELAPLARELELCRQYLNLEQ
                     LRLGERLKVEWRTERMPRDAMVPLLVLQPLIENAVYHGVEPRVEPGVVSIDIYATDDL
                     LHAVLRNPYQEKTGPHSGNRMALANIRERLQLHFDAEATLTTRVADGIYQVHIVMPYI
                     KGREP"
     gene            3805..5199
                     /gene="argH"
                     /locus_tag="AC158Q_00865"
     CDS             3805..5199
                     /gene="argH"
                     /locus_tag="AC158Q_00865"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HIR3338349.1"
                     /translation="MSVNDKSRNQPWSGRFGEPATELVQRFTASVSFDRRLAEFDIEA
                     SIAHARMLRAVEIISKNDLAEIEKGLTQIRAEIREDRFPWSEQHEDVHFNIERRLTDL
                     VGEPGKRLHTARSRNDQVATDVRLYLRAAIDQAVATIKSVQRALLDLADAHAETVMPG
                     YTHLQVAQPVSYGHHLLAYFEMLARDAQRLADCRKRLNRLPLGSAALAGTSFPIDRQM
                     VAKELGFDGVCENSIDAVSDRDFAIEFCACAALVMTHLSRFCEELVLWMNPRFGFVAL
                     ADRFCTGSSIMPQKKNPDVAELIRGKTGRVNGHLVALLTIMKGQPLAYNRDNQEDKEP
                     LFDTVDTLTMSLAVFAELLPGIQVNREAMEAAARQGHATATDLAEYLVKKGVPFREAH
                     EAVAQAVRYAESRGCELADLKVSELQRFSGAIGKDALAVLTLEGSLMSRTHLGGTAPV
                     RVKAAIAKARKSLA"
     gene            5331..>5555
                     /locus_tag="AC158Q_00870"
     CDS             5331..>5555
                     /locus_tag="AC158Q_00870"
                     /inference="COORDINATES: protein motif:HMM:NF015818.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HIR3338350.1"
                     /translation="MSPWKDIERAIREASGAPFAIESRAGVGGGCINECHVIRGGGHA
                     YFVKLNVPGKTEMFSAEAAGLNEIAGTRTVR"
BASE COUNT          949 a         1869 c         1807 g          930 t
ORIGIN      
        1 gcgttgatca gtttcatcga gcgctcgacc gaattggggc tgatgaagat cgcgtagtcg
       61 aactcatcga gccggtcgat caactgcgtg aacggacgca ggtcctcgac atcgcggatc
      121 tcgatcaccg gaaacaggac cggacggccg cccgcgccct cgatcaggcg ggcaagcgcc
      181 tgcgcctgct gcgtcggccg cgtcaccacg atgcctttcc ccgcaagcgg cctgcccttc
      241 gtcgcttcac tcacgagaat ctcccgccgc ctgctgaagg gcgtgaatgg tgaatggtgt
      301 tcagcttgcg tctcccgctt tatcgggcgg cggattgccg ctccagttcg gccaggatgc
      361 cggtggcgcc ctgcgccttc aggcgcgccg cgagcgcggc gccgagcgct tccggctcgt
      421 ccgccgggcc atcctgctcg ccgttcacca tgcgcgagcc gtcgggcgca ccgacgaatc
      481 cgcgcagccg gatccgtttc ccttccattt ccgcgtaagc cccgagcggg aggttgcagt
      541 tgcccgagag cgcgcgcgag aaggaccgtt ccgcttcaac gcaacgcgcc gtggcggcat
      601 gggcgagggg cgcaagctgg ccgatcaggt cggtgcgatc cgccagacac tcgatgccga
      661 gtgcgccctg tcccggtgcc ggcaggctct cggagggcgc gagcagcagc gtaatgcggt
      721 cctccagccc cagccgcttc aatcccgcgg cggcgactat cacggcttcg tagttgccat
      781 cgtcgagttt cttcaaccgg gtcgggacgt tgccgcgcag tggttcgatt ctcagccgcg
      841 gatggcgcgc gcgcagctgg cattcgcgcc gcaggctgga ggtgccaacc cgggcgcccg
      901 ccggcagggc gtcgaggccg gcatagcggc tggaaacaaa tgcgtcgcgc gggtccgcgc
      961 gttcgccgat cgcggcaagg acgaagccgc gcggcagctc catcggcacg tctttcaccg
     1021 agtgcaccgc gatatcggcg cgcccgtcgg ccagcgcatc ctccagttcc ttgacgaaca
     1081 gtcccttgcc gcccatgttc gcgagcgagg catcgagacg gcggtcgcct tcggtcgtca
     1141 tgcccagtat ctcgacgccg gccccggggt acaacgcggc aagctgcgac tggatatggc
     1201 gcgcctgcca cagcgcgagc gcgctctcgc gcgaggcgat cacgatccgc cccggtgcgc
     1261 tttcagttga accggctgcc gtcatgcggt tggagaatac aggaatcgcg aattgtgcgg
     1321 cgcatcgccc atcgcccatt attcatcacg ccttggtgcg atcagcggca gacctgcggc
     1381 tgatggccgc tctgcttgcg cttcaccgcc gcgccgtagc ccgccagctc gaacgatacg
     1441 aacaccaggt ccttgccgcc gtaggcgatg atgctcgaat cggcattgcg ccagctggaa
     1501 gccggatagc cgccggccgg cgccggttcc atccgctggg gggcgccgtg ccgctcgcgc
     1561 aacgcctgca tcaccgcttc gtactgccgg atttcggcgg tgatcgagaa ctgctgcagg
     1621 ataccgtcga ggaaatggag acggacctcc cgcgtctcga ccccatcgac ccgttcgtcg
     1681 tgcgacacgc aggcgatatc ggcgaacggg tcttcgcggc gcgcgcaacc gtaaccgcgc
     1741 cgcccgagat cgggtttggc cgacttagtc tccttttgcg cggcgatcgc cgagtggatg
     1801 tagcggaagt cgagtgcggc ggcgagtctt gcataacttg cgcccagagc aatatcatcg
     1861 agcccgtacg gctgatccgc tccgtgcgcc gccgcaaata taagaaacga caaacagacg
     1921 gcctcgactt tgaggacttg attcttgtag atcatccctt tattcggaaa agcgtcaggc
     1981 ttttccgaat tcgcgcacga tgtactgctg gcgccggctg acggcgatgc gctcgtcggc
     2041 gccggcgagg accacctccc agtgcgtctc accgccctcc tccgagcgct cgaacccgcg
     2101 gatcgccgcg cgcgcgacca ggcagttgcg atgcacccgc acaaaacggt ctccgaattc
     2161 ctgctcgagc cgggtgagcg actcctcgat caggtattcg cgggtggtgg tgcgcaccga
     2221 cacgtacttg agctcagcct tgaggtagat cacgtccgca atcgggatca ggtggatcct
     2281 gccgcgctcc tgggcgcaga ggtgtgtgcg cggggcgcgt gccagttcgc gcagcgcttg
     2341 cggacgcggc ggcgccgcgc gcgcgcgcgc cagcgcttcc ctgagacgct ccagccgtat
     2401 cggcttcaac aggtagtcca ccgcgtgcac ctcgaacgcc cggatcgcgt agtcgtcgta
     2461 agcggtggcg aacaccaccg acggcggacg ctcgagttgc tgcaggtgca gcgccacctc
     2521 gatgccgtcc atttccggca tgcgaatgtc gagcagcacc acgtccacgg ccgaccgcga
     2581 cagcagatcg agcgccgccg gcccgctcgc cgcctcgccg gcgacgatga gggggggttc
     2641 gccgccgcat tcgccgagga ggtcgcgcag gcgcagccgc gccggcgctt cgtcatccac
     2701 gatcattacc cgcaaggacc gctcgtcatt catggctcgc ggcccttgat gtacggcatc
     2761 acaatgtgca cttggtagat gccatcagcg actctcgtcg tgagggtggc ttccgcgtcg
     2821 aaatgcagct gcaggcgctc gcggatattg gcaagcgcca tgcggttgcc ggaatggggg
     2881 ccggtttttt cctggtacgg attacgcagg accgcgtgca acaggtcgtc ggtggcgtag
     2941 atgtcgatgc tgaccacgcc gggctcgacc cgcggctcga cgccatgata gaccgcgttc
     3001 tcgatcagcg gctgcagcac gagcaacggc accatggcat cgcgcggcat ccgctccgtg
     3061 cgccattcga ctttcagccg ctcgccaagg cggagctgct ccaggttgag gtactggcgg
     3121 cacagttcca gctcgcgtgc gagcggcgcg agttcccggt tgtcttccat cagcatgcga
     3181 aacagcccgg ccatgtcctc gagcgcggct tccgcgcggc ggggctcctg gcggatcagc
     3241 gagagcaccg cgttgatgct gttgaacagg aagtgcggtc ggatgcggga ctgcagcgcc
     3301 tgcagccgcg cttccgtcag cgccggcgac agcgcccggc cgcgcgcgtc gaagtagacg
     3361 agcagcgccc ccgtggtcaa tgcgacgagc agccagtagc gctccagcgg cgcgggatcc
     3421 ggccccagca gggtccggcc cgcaacgtag agccccgtcg tcagcgcgag ttcgagcacg
     3481 agcacggcgg ccgcgccgag ccagtacggc atgcgccgca acccgccgtt cagcgcagcg
     3541 agcacgagca gggagagtat gagcagcggc tcgaccagcg aggcgacctg gatggtctgc
     3601 tgccacagcg ctcccaggtc gtggctcttc acgatcgcca cggcgaacgc gaacacgttt
     3661 acgagcacac cgatgcgcag caggatgccg agattgcgaa agttcggcag gccgccggta
     3721 taggcgtttt gatttatact tcttgccatg cgcgccttgc gctcctaaca cgatgtttca
     3781 tctggtcaaa ttctaacagg ccgcatgtcc gtcaacgaca aaagcagaaa ccagccgtgg
     3841 tcgggacggt tcggcgagcc ggcgaccgag ctggtgcagc gtttcaccgc ttccgtctcc
     3901 ttcgaccggc ggctcgccga gttcgacatc gaggcctcga tcgcccacgc gcgcatgctg
     3961 cgcgcggtgg aaatcatcag caagaacgac ctcgcggaaa tcgagaaggg cctcacgcag
     4021 atccgcgccg agatccgcga ggaccgtttc ccctggtcgg agcaacacga ggacgtgcac
     4081 ttcaacatcg agcggcgcct gaccgacctg gtcggcgagc ccggcaagcg cctgcacacc
     4141 gcccgctcgc gcaacgacca ggtcgcgacc gacgtgcgcc tctacctgcg cgccgcgatc
     4201 gaccaggcgg ttgccaccat caagtccgtg cagcgggcac tgctcgacct cgcggatgcg
     4261 catgccgaaa cagtgatgcc gggttacacg cacttgcagg tggcgcagcc ggtgtcctat
     4321 ggccaccacc tgctggcgta cttcgagatg ctcgcccgcg acgcgcaacg gctcgccgac
     4381 tgccgcaagc ggctgaaccg cctgccgctc ggatcggcgg cgctcgccgg cacctcgttc
     4441 ccgatcgacc gccagatggt cgccaaggag ctgggtttcg acggcgtgtg cgagaactcg
     4501 atcgacgccg tgtccgatcg cgacttcgcc atcgaattct gcgcctgcgc cgcgctggtg
     4561 atgacacatc tgtcgcgctt ctgcgaggag ctcgtcctct ggatgaatcc gcgcttcggc
     4621 ttcgtcgcgc tcgcagaccg cttctgcacc ggctcctcga tcatgccgca gaagaaaaac
     4681 ccggacgtgg cggagttgat ccgcggcaag accgggcgcg tgaacggcca tctggtcgcg
     4741 ctgctgacga tcatgaaggg ccagccgctc gcctacaacc gcgacaatca ggaagacaag
     4801 gaaccgctgt tcgacacggt ggatacactt accatgagcc tcgctgtgtt cgcggaattg
     4861 ctgccgggca tccaggtcaa ccgcgaagcg atggaggccg ccgcacgcca gggacacgcc
     4921 accgccaccg atctcgccga atacctggtg aaaaaaggcg tgccgttccg cgaagcgcac
     4981 gaggcggtgg cacaggccgt gcgctacgcc gagtcgcgcg gttgcgagct ggccgacctc
     5041 aaggtctccg agctgcagcg cttctcgggc gcgatcggca aggacgcgct cgccgtgctg
     5101 acgctcgagg gttcgttgat gagccgcacc catctcggcg gcaccgcccc ggtgcgcgtg
     5161 aaggcggcga tcgccaaggc gcggaaatcg ctcgcttgaa atttagccgc cgatgaaagc
     5221 cgataaacgc cgatgagaat ctagaacaag gacggcttac ggcagtccgg gttcttgtcc
     5281 gagcgggcgc cgcatcggcg tccatcggcg gttccatcca ggttttttgc atgagcccat
     5341 ggaaggacat cgagcgcgcg atccgcgaag cgtcaggcgc gccctttgct atcgagtccc
     5401 gtgcaggagt cgggggcgga tgcatcaacg aatgccatgt catccgcggc ggcgggcatg
     5461 cgtacttcgt caagctcaac gttccaggga aaaccgaaat gttttccgct gaagcggccg
     5521 ggttgaacga aattgcgggc acgaggacag tccgt
//
LOCUS       DBNGSG010000039         3548 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1879427, whole genome shotgun sequence.
ACCESSION   DBNGSG010000039 DBNGSG010000000
VERSION     DBNGSG010000039.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1879427"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /gene="ssrA"
                     /locus_tag="AC158Q_00875"
     tmRNA           <1..348
                     /gene="ssrA"
                     /locus_tag="AC158Q_00875"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(624..827)
                     /locus_tag="AC158Q_00880"
     CDS             complement(624..827)
                     /locus_tag="AC158Q_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015004218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR3338351.1"
                     /translation="MATGTVKWFNDAKGFGFITPDDGGEEVFAHFSEIQGSGHKSLKE
                     KQRVSYEVTRGQKGLKAAAIKPL"
     gene            complement(1028..1687)
                     /locus_tag="AC158Q_00885"
     CDS             complement(1028..1687)
                     /locus_tag="AC158Q_00885"
                     /inference="COORDINATES: protein motif:HMM:NF022337.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HIR3338352.1"
                     /translation="MEVNVRLVVPVARMIAWLLFLLIVGSIVAYCVWDYRKKAAERAA
                     ASMERFSQMFEAKAIAAPAPAPESPSKAAPVTGARAIPGAPPAAVTVLHGRERFLGKA
                     ETLLYYLLKTGIPGHEVFANVTLAAVIEPSGGRFEQEQQLRHLSQYRLDFVVCDKSMR
                     VVAAVELEALGGADAAGAQLFKSDNLKAAGVRLIRINPKALPRREELRAQVCGPDAAG
                     A"
     gene            complement(1645..1950)
                     /locus_tag="AC158Q_00890"
     CDS             complement(1645..1950)
                     /locus_tag="AC158Q_00890"
                     /inference="COORDINATES: protein motif:HMM:NF025363.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK family protein"
                     /protein_id="HIR3338353.1"
                     /translation="MSGIQDQLIEIARRKERLIARTEAQRAAIGESFQQLQGPIGVVD
                     RGLEIARFLRGHPLLVAAVVAATIAFRRRGVVAVVGSLLTAWRLWKSMSAWSSRLLA"
     gene            complement(1947..2339)
                     /locus_tag="AC158Q_00895"
     CDS             complement(1947..2339)
                     /locus_tag="AC158Q_00895"
                     /inference="COORDINATES: protein motif:HMM:NF018980.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HIR3338354.1"
                     /translation="MSESAPGEAGAGIVQSLRSLAATLVALLRTRAELLATEIEEERV
                     RLLQLLVWAAAALFFLGVGVLLLVILYVAAFWDSYRIPAIVALAVIFLAAGAGMAIGV
                     RNRMYARPRLFSASLDELARDKDQLTPR"
     gene            complement(2352..2666)
                     /locus_tag="AC158Q_00900"
     CDS             complement(2352..2666)
                     /locus_tag="AC158Q_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ElaB/YgaM/YqjD family protein"
                     /protein_id="HIR3338355.1"
                     /translation="MTAPDTTATKEKLAQDLKIVISDAEELLRATASQAGEKAAAARE
                     KIQDSLRNAKLKLAEVEDILMDRGKQAARATDEYVHDHPWRAVGIAAGVGLIIGMLIS
                     RR"
     gene            complement(2809..3387)
                     /locus_tag="AC158Q_00905"
     CDS             complement(2809..3387)
                     /locus_tag="AC158Q_00905"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HIR3338356.1"
                     /translation="MRSVCVFCGSNTGTGGSYATAARELARAVAARRLRLVYGGGSIG
                     LMGALGEAALAAGGHVIGVTPRQLVEREVVHAGLTELRVVETMHERKALMAELSDGFI
                     ALPGGLGTLDELFEMLTWTQLGIHRKPCALLDVEGYFGKLAAFLDHAVVERFIRAEHR
                     AMLITGRNPETLLDQLASIRLPEISKWMDRKS"
BASE COUNT          662 a         1194 c         1076 g          616 t
ORIGIN      
        1 cgcgggtcac gaagcagcgc agtgcatacc gaggaccagt tccctcgtaa aaccactgga
       61 aacttcatag acgccaacga cgagcgttac gctctcgccg cctaacaccg gcgagacgct
      121 gcactagcct gctgatgggc taggccggct tcggccggtg cagcgccatt cacatcagcc
      181 tctctgcgcg gtctggtcgc ttggccgtgt ggatccgtcg aagcgactgg ctgagcgtca
      241 gcctgccggc cggcgggcgc gaggcgagat ccaaacgacc tggctaagca tgtagtcctg
      301 gctgtgtagg gcttgcggac gcgggttcaa ttcccgccgc ctccaccatc acgcggaaaa
      361 agggctaccc cgtcgggggc ggcccttttc gtttgatgag gccgcgggga ttgcagccgt
      421 gtccggcacg gacgcggggg cgagtcggac acgggacagt gcctgcgcac tgtctcgtgc
      481 ctacgagccg gctcgcacct gccggtgcga gcgcggcctg caaggccaat tcccgccgcc
      541 tctaccacca ataaaaaagg ggctcgttcg agcccctttt cttttgacgg ccgcggcact
      601 gagccgcggc tgtcgcccgg aaattacagc ggcttgatcg cggctgcctt caggcccttc
      661 tgtccgcgcg tgacttcgta gctgacgcgc tgcttctctt tcagcgactt gtggccgctg
      721 ccctgaatct cggaaaaatg cgcgaatact tcctcgccgc cgtcatccgg ggtaatgaac
      781 ccgaaaccct tcgcgtcgtt aaaccacttg acggtacccg ttgccataca actctcctat
      841 acgaaaatga atgaacgcgg actcatgccc gcactgcgca tacgaatcaa gaagtggttt
      901 tgagaacgag acactccacc cgaacgggag aaggactacg gatcagcaat actccgcttg
      961 atacgactgc ggccgaacaa tacccacatt ccggaaaaat ggcaagtcac gccgctactt
     1021 ccgcgcgtca ggctccggcc gcatccgggc cgcagacctg ggcgcgtaat tcctcccgcc
     1081 gcggcaacgc tttcgggttg atccggatca ggcgcacacc ggccgctttc aggttatcgg
     1141 atttgaaaag ctgcgccccc gccgcatccg caccgcccag tgcttccagt tcgacggccg
     1201 ctacgactcg catgctcttg tcgcatacca cgaagtcgag ccggtactgg gacaggtgcc
     1261 gcaactgctg ttcctgctcg aacctgccgc ccgatggctc gatcaccgcc gcgagcgtca
     1321 cattcgcaaa cacttcatgg ccagggatcc cggtcttgag caggtagtag agcaatgtct
     1381 cggccttgcc gaggaaacgc tccctgccgt gcagcacggt gaccgcggca ggcggtgcgc
     1441 ccggaatggc gcgcgctccc gtcaccgggg cggccttgga aggcgattcc ggcgctggcg
     1501 ccggcgcggc gattgctttt gcctcgaaca tctgcgagaa gcgctccatg ctggcggcgg
     1561 ccctctccgc ggcttttttg cgatagtccc aaacgcagta ggcgacgatc gaacccacga
     1621 tcagcaggaa tagaagccag gcgatcatgc gagcaaccgg gacgaccagg cggacattga
     1681 cttccacaag cgccacgccg tgagcaggct gccgaccacg gcaaccaccc cacggcgacg
     1741 gaaggctatc gtagcggcaa cgacggcggc aacgagtaat ggatgcccgc gcagaaatcg
     1801 cgcaatttcc agcccgcggt cgacgacgcc gatcggcccc tgcagttgct gaaaactttc
     1861 gccgattgcc gcgcgctgcg cctcggtgcg cgcgatcagc cgctccttgc gccgtgcgat
     1921 ctcgatcaac tggtcctgga taccgctcat cggggtgtca gctggtcctt gtccctggcc
     1981 agctcgtcga ggctggcgga aaacaaccgg gggcgggcat acatgcggtt acgcacgcct
     2041 atggccatgc cggcgcccgc cgccagaaaa atgaccgcga gcgcaacgat ggccgggatg
     2101 cggtaactgt cccagaaagc agccacgtac aggatgacca gcagcagcac gccgactccg
     2161 aggaagaaca gtgccgcggc cgcccagacc agcagctgca ggagccgtac gcgctcttcc
     2221 tctatttcgg tcgcaagcaa ctcggcgcgg gtccgcagga gcgcaaccag cgttgccgcc
     2281 aggctgcgca acgactgcac gatgccggcg ccggcttccc ctggagcgga ttctgacacg
     2341 gcctgaccgt actagcgccg gctgatcagc atgccgatga tgaggccgac gccggcggca
     2401 atgcccaccg cccgccacgg atgatcgtga acatactcgt cggtggcgcg cgcggcctgc
     2461 ttgccccggt ccatgaggat atcctcgact tcggcgagct tgagcttggc attgcgcagg
     2521 ctgtcctgga ttttctcgcg cgcagcagcg gccttctcgc ccgcctgact ggcggtggcg
     2581 cgcagtaatt cctcggcgtc ggaaatcacg atcttcaggt cctgggccag cttttccttg
     2641 gtggcagtgg tgtcgggggc ggtcatggca tcctcttggc agggggagtt gatacagggg
     2701 caaaccagag gcgataaggc taccgacttt cccctgcgaa ctcaaccgcc tcggtgcgca
     2761 cccggcaccg ccggcccgca cggatgtcgt caggcggagt cgatggcatc aggacttgcg
     2821 gtccatccac ttgctgattt ccggcaagcg aatcgaggcc agctggtcga gcagcgtctc
     2881 gggattgcgc ccggtgatca gcatggcgcg gtgttccgcc cggatgaaac gctccaccac
     2941 cgcgtggtcg agaaatgccg ccagctttcc gaaatatccc tcgacatcga gcagcgcgca
     3001 aggcttgcga tggatcccga gctgggtcca ggtcagcatc tcgaacagtt cgtccagcgt
     3061 gcccagtccg cccggcaagg cgataaatcc gtcggacagc tccgccatca gcgccttgcg
     3121 ttcatgcatg gtctcgacga cgcgcagctc ggtaagccct gcatgcacca cttctcgttc
     3181 cacgagttgc cgcggcgtga cgccgattac gtggccgcct gcggccagtg ccgcctcgcc
     3241 gagcgcgccc atcaatccga tgctgccccc gccgtaaacc agccggagac ggcgggccgc
     3301 caccgcccgc gccagctcgc gcgccgcagt ggcataagac ccgccggtcc ctgtgttcga
     3361 accgcaaaaa acgcaaacgc tgcgcaccct gccccctggt gaaaagcctt tattctatgc
     3421 cccgccaact cggatcccga tccggcgatt tttatatgct gctggtatag aatccctgga
     3481 acaagccgct ccggctgcaa accaaccgga aacctgccgg agactgaggg ggcatcatgg
     3541 gaatagcg
//
LOCUS       DBNGSG010000040         3849 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3747533, whole genome shotgun sequence.
ACCESSION   DBNGSG010000040 DBNGSG010000000
VERSION     DBNGSG010000040.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3747533"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..333
                     /locus_tag="AC158Q_00910"
     CDS             145..333
                     /locus_tag="AC158Q_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338357.1"
                     /translation="MKKLLAALFAGLFAVTLSSPAIAQGKKETAKMEKKKGAEKKTDG
                     ATKSGDKKKTGKKEAPKK"
     gene            431..895
                     /locus_tag="AC158Q_00915"
     CDS             431..895
                     /locus_tag="AC158Q_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR3338358.1"
                     /translation="MPSNKDIWKPSVTVAAVVERDGRFLLVEEDTAHGRLFNQPAGHL
                     DPGESLIRAVERETLEETAYAFKPTGLLGIYQYHSGADDVTYIRFAFTGEITGHDAGR
                     ALDTGIVRAAWIAPDEIRREAGRHRSPLVMRCIDDYLAGRRHPLAVLHHHPA"
     gene            901..2076
                     /gene="mnmA"
                     /locus_tag="AC158Q_00920"
     CDS             901..2076
                     /gene="mnmA"
                     /locus_tag="AC158Q_00920"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010091371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HIR3338359.1"
                     /translation="MAGKRVIVGMSGGVDSSVAALLLKQQGCQVSGLFMKNWEDDDTD
                     EYCSSREDLIDAVAAAERIGIEIDVVNFSAEYKSRVFNDFLREYQAGRTPNPDVLCNA
                     EIKFRAFLDHALGLGADVIATGHYAQVREVDGLHQLLMAEDGTKDQSNFLYRLNQSQL
                     ARTLFPLGKLYKRDVREIARREGLPNHDKKDSTGICFIGERPFREFLSRYLPDNPGEI
                     RTPEGRLVGRHQGLSFYTIGQRQGLGIGGNRNGGGEPWYVAAKDLRGNRLTAVQGHDH
                     PALLSDRLTALDLNWISGAPPRCHWVYGAKTRYRQKNAPCTLVAASAERCVVEFAEPQ
                     WAVTPGQSVVIYESRVCLGGGIIESGERTREAESRQEECPPGTVDPGDEGGGMRAEE"
     gene            2336..3043
                     /locus_tag="AC158Q_00925"
                     /pseudo
     CDS             2336..3043
                     /locus_tag="AC158Q_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012675.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
     gene            complement(3053..>3849)
                     /locus_tag="AC158Q_00930"
     CDS             complement(3053..>3849)
                     /locus_tag="AC158Q_00930"
                     /inference="COORDINATES: protein motif:HMM:NF024260.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR3338360.1"
                     /translation="DGRLILQVAGEPPLELRGGAIVLLPRNDAHTLASQEGLKPIGGD
                     QLPHEDTGVGPIRIRYGGGGERTHIVCGLLGTDSRNHPLLDTLPPTLVLDLKGDPACE
                     WIARSFRYAAGELAAVRAGSGIALSKLSELLFVEAVRRYLDTLPADRRGWLAGLRDPV
                     VARALALLHTHLARGWTTEALAQEVGASRSAFAERFTALMGLPPMRYLTGWRMQVATE
                     RLRDTRRSVAQIAAEVGYESEIAFARAFKREFSASPAQWRKQPLKS"
BASE COUNT          727 a         1268 c         1267 g          587 t
ORIGIN      
        1 aacccctgcc tttttttatg gaccggcgcg taccggtcat gacattgcca tagacagtga
       61 tttggactag gctggctccg cgagaaaggg gctccggggc tcgcacgccg gagacgatcg
      121 atgaacccgt agggaggaaa taagatgaaa aagctgctcg ctgcattatt cgccggcctg
      181 ttcgccgtga ccctgtcatc gcccgcgatc gcccagggca agaaggaaac cgcgaagatg
      241 gaaaagaaga agggcgcgga gaagaagacc gatggcgcga cgaagagcgg ggacaagaaa
      301 aagaccggca agaaggaagc cccgaagaag taacgggatc gttccgcggc aaagggcagg
      361 gatcgaaccc tgcccttttt gtttgcgccg tcactgcctg tgcggcgccg cgcggcgata
      421 aaatagcgcc gtgccgtcca acaaggacat ctggaaaccg agcgtcacgg tcgccgccgt
      481 ggtcgagcgc gatggccgct ttctcctggt cgaggaagac accgcgcacg ggcgcctgtt
      541 caaccagccg gccggacacc tcgacccggg cgaatccctg atccgcgccg ttgaacggga
      601 aacgctggaa gagaccgctt atgctttcaa accgaccggc ttgctgggga tttaccagta
      661 tcactccggc gcggatgacg tgacttacat ccgttttgcg ttcaccggcg agatcaccgg
      721 gcacgacgcc ggccgcgccc tcgataccgg catcgtgcgc gccgcctgga tcgccccgga
      781 tgaaatccgg cgcgaagccg ggcgccatcg ctccccgctg gtcatgcgct gcatcgacga
      841 ttacctcgcc ggccggcgcc acccgctcgc cgtcctgcac catcaccccg cctgaacccc
      901 atggcaggca agcgcgtcat cgtcggcatg tcgggcggag tggactcgtc ggtggccgcg
      961 ctgctgctca agcagcaggg ctgccaggtc tcgggcctgt tcatgaagaa ctgggaggat
     1021 gacgataccg acgaatactg ctcctcgcgc gaggacctga tcgacgccgt cgcggcggcc
     1081 gagcggatcg gcatcgaaat cgatgtcgtg aatttctcgg cggaatacaa gtcgcgcgtg
     1141 ttcaacgatt ttctccgcga gtaccaggcc ggccgcacgc ccaatcccga cgtgctgtgc
     1201 aacgccgaga tcaagttcag ggccttcctc gaccacgcac tgggactcgg cgccgacgtc
     1261 attgccaccg gccactacgc gcaggtgcgc gaagtcgacg gcctccacca gctcctgatg
     1321 gccgaggacg ggaccaagga ccagagcaac ttcctctacc gcctgaacca gtcgcagctc
     1381 gcgaggacgc tgtttccgct cggcaagctc tacaagcgcg acgtgcgcga aatcgcccgg
     1441 cgcgaaggtc tgcccaacca cgacaagaag gactccaccg ggatctgctt catcggcgag
     1501 aggccgttcc gcgagttcct ctcccgctac ctgccggata acccgggcga gatccgcacc
     1561 ccggaaggcc ggctcgtcgg gcgccaccag ggcctgtcgt tctacaccat cggccagcgc
     1621 caggggctcg gcatcggcgg caaccgcaac ggcggcggcg agccctggta cgtcgccgcc
     1681 aaggacctgc gcgggaaccg gctgaccgcc gtgcaggggc acgatcaccc ggcgctgctc
     1741 tccgaccgcc tgacggcgct ggacctcaac tggatcagcg gggcgcctcc gcgttgccac
     1801 tgggtgtacg gcgcgaagac gcgctatcgc cagaaaaacg cgccttgcac gctggtggcg
     1861 gcgagcgccg agcgctgcgt ggtcgaattc gccgagccgc aatgggcggt cacccccggc
     1921 cagtcggtgg tgatctacga gagccgggta tgcctgggcg ggggcatcat cgaatccggc
     1981 gagcggacgc gcgaagcgga atcgcggcag gaggaatgcc cgccgggcac ggtcgatccg
     2041 ggggacgagg gcggagggat gagggcggag gaatagtcaa aatagcgcaa gccgtttcct
     2101 catccatccg gcccgacacg ctgcatcgtg ccgggcgttc gccggctcga acgtgagcag
     2161 gcacgttatt cgaaaccccg gctcgctcat tcatccccga ttcatatcgc cttgacgctt
     2221 gccggaagcg gagagtagtc ttgattgaac actgatcgag gtccgcaata tgggcgccat
     2281 ggacgaaaag ttcatcctgt ttctccagcc tgggtgaagc agctgtcttc gagcgaaaga
     2341 gtttctttcg aaaaagaaga tagaccacac cgtcatcgac gttgccaatg atcccggcgg
     2401 gcgggaaaag ctcctcgctc tcggcgttcg caacgtgccg gtcgtgtcga agggcgggaa
     2461 ctacgtcttc gggcagaacc tcgaggacgt ggccgagttc gtcgggctcc agggcaccgg
     2521 gcacaagccg ctgccgccgg aggaactcat caggaaatgg gttctcgtgc tgcgcgccct
     2581 gcaacgctac gcgcgccaga tgccggccga gcgcatcaac gagaacgtca tcgagaaccg
     2641 gcagcgctcg atccggctgc tcgcgcatca cgcgttccgc atcgccgagg cgtacctcga
     2701 gtccgtgatc gacggcaagg aatacgcggt tcagcacgcc aacgtgccgc cgaaagacgg
     2761 cacgcacctg accggcgagg agatcgccgc ctacggcgag ggcgtgatcg ggcgcctgca
     2821 gcaatggtgg gaccaggtcc cggacaggac gctttcctcc aggatgaaga cctattacgg
     2881 catgcagccg atggcgatag tgttcgagcg ctcgacctgg cactcggcgc agcacgcccg
     2941 tcagttgatc cacgtgctcg agcgtttcgg catcgagccc gacggacggc tcacgccgca
     3001 ggacctggcc ggactgccgt tgcccgagcg gctgttcgag taatccggcc catcacgatt
     3061 tcagcggctg cttccgccac tgcgccggcg aggcggagaa ctcgcgcttg aacgcgcgcg
     3121 cgaaggcgat ttccgactcg taacccacct ccgcggcaat ctgcgcgacc gagcggcgcg
     3181 tgtcgcgcag gcgttctgtg gctacctgca tgcgccagcc cgtgagatat cgcatcggcg
     3241 gcaggcccat cagggccgta aagcgctccg cgaacgccga gcgtgaagca ccgacttcct
     3301 gcgcgagcgc ctcggtggtc caaccgcgcg ccaggtgggt gtgcaggagg gcgagcgcgc
     3361 gcgccacgac cgggtcgcgc agtccggcga gccagccgcg gcggtccgcg ggcagcgtgt
     3421 cgaggtagcg ccgaacggcc tcgacgaaca gcagctcgga cagcttggaa agcgcgatgc
     3481 ccgagcccgc gcggacggcg gcgagttcac cggcagcgta gcgaaacgat ctcgcgatcc
     3541 actcacacgc cgggtccccc ttgagatcga gcaccagcgt cgggggcagt gtgtcgagga
     3601 gcggatggtt ccgcgaatca gtgccaagca gaccgcagac gatgtgggtc cgctcgccgc
     3661 cgccgccata gcgaatgcgg atcggaccca ccccggtatc ctcgtgcggc agttggtcgc
     3721 cgccgatcgg cttcaacccc tcctggctgg cgagcgtgtg ggcatcgtta cgcggcagca
     3781 gcacgatggc gcccccgcgc agctccaggg gaggctcgcc ggcgacttgc aggatcagcc
     3841 tgccgtcca
//
LOCUS       DBNGSG010000041         7800 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2898543, whole genome shotgun sequence.
ACCESSION   DBNGSG010000041 DBNGSG010000000
VERSION     DBNGSG010000041.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7800)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7800)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7800
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2898543"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /locus_tag="AC158Q_00935"
     CDS             <1..881
                     /locus_tag="AC158Q_00935"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020039.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR3338361.1"
                     /translation="LETPSAERLATGFVFTEGPLWHPGGFYYFVDIRQNKLYRITPGE
                     KAEVVRETAGGNGTTFDLKGNLILCEGDGRRVTRMGADGRIETVADNYKGGRFNRPND
                     VVCRSDGSLYFTDPDKRVPYGERQIPAPAGENNLWDGARVYRVAADGGLSVLAHCEYP
                     NGLALSPDERTLYVANTRSTMYVHAIELDAAGVMARRRIFADMNEGAAPGIPDGLKVD
                     SQGRVYCTGPGGIWVFTPDGKRVGIIRFPEQAVNFAFGGPDLKTLFCCAHTSVYTLRV
                     KVAGNPHPWYRLRKQG"
     gene            complement(966..1235)
                     /locus_tag="AC158Q_00940"
     CDS             complement(966..1235)
                     /locus_tag="AC158Q_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338362.1"
                     /translation="MRAKALHWITPLALAGLGLLAAGVYFFFFTRVDSYGRVIGLTFA
                     ASGLVAIVVKCALLVLLQRKMGWIWAAELALVAALFIYARVNGLP"
     gene            complement(1353..3320)
                     /locus_tag="AC158Q_00945"
     CDS             complement(1353..3320)
                     /locus_tag="AC158Q_00945"
                     /inference="COORDINATES: protein motif:HMM:NF016906.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HIR3338363.1"
                     /translation="MNTRSFSAALHYLAARLRRAPLLCLASVLAQPAWAATYYVATNG
                     NDGNSGSSVTQPFRTIQRGMNAVVPGDTVLVRGGTYREQVEATRGGTADKPVPVSAYP
                     GEIPVIKGSDVVTGWVRDGTVVWKKTGWPHNSQQVFADFNAKPKKSLQQIGMPSQFYT
                     SWEYPSPVGTGRASMTAGSFYYDPASAALYVRLADGSDPNRRVMEASVRRRLFIMHQP
                     HIHLKGFRFRHSSLSAFAQQGAAVELSSHSSIENCDIQYVDFAGLSMGYLQDGAHAHN
                     CVISNNGNSGINAAGSTNFWVTSVTLMHNNSRNFNVNWHAGGFKGAAKAYGTIENSEI
                     ANNNGAGIWFDYANGSWPIVIRNNYVHDNGPSEGAIFFEVSNNGLIYNNVVANNRKRG
                     IYLSAANNTRVYNNTIYGTSERAGIEVGGMPRGSSTLTNNSVQNNIVSHGSSAYDLYI
                     VPANGTTIAGNASDYNNFFRPAGAINLRYSATYQNLAGWVAATGMDAHSLSANPGFVA
                     PGASASGYAVTTGSPVIDAGANLVSVVPDDYLKVFRPSGAAADIGAFEFDLSAVVPAA
                     TADTIPPMVTINSAVATTTTSLTISASATDNVGVTGMQLYIGGVLKTSSMNGQVSYAW
                     NNPTPGSYELRITADDAAQNTGSATATLTIQ"
     gene            3964..4254
                     /locus_tag="AC158Q_00950"
     CDS             3964..4254
                     /locus_tag="AC158Q_00950"
                     /inference="COORDINATES: protein motif:HMM:NF044585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6812 domain-containing protein"
                     /protein_id="HIR3338364.1"
                     /translation="MDGQESRTKVVILTGSYRIKGYIDLLPGARVTDYILGAKNFIVV
                     TEAEIWSIGGNGLVLSAQFVDVSRDRIEVITTTDRQKSLAAVADLEHVAAVS"
     gene            complement(4316..4795)
                     /locus_tag="AC158Q_00955"
     CDS             complement(4316..4795)
                     /locus_tag="AC158Q_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338365.1"
                     /translation="MKDELSRLLKAHGAKRRQEKDVEEAEEKGHQVFQALAVDTIEKV
                     IAPALEALSQELKGHGHEADVSLRVGTEAYPSAHLSFRIINRDDPLDPASASRLSFTT
                     TPSQDRFEVRTEIWGREGKETGLINTGNPETRRIAEIDAGWVTAQGLSFVGCVLDRA"
     gene            5053..5238
                     /locus_tag="AC158Q_00960"
     CDS             5053..5238
                     /locus_tag="AC158Q_00960"
                     /inference="COORDINATES: protein motif:HMM:NF016473.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIG1 domain-containing protein"
                     /protein_id="HIR3338366.1"
                     /translation="MSMITAMILFALLATIGSLAAGVTSMATNAEVGHLRSEQWMGWR
                     VAFQAVALLFILIALLT"
     gene            complement(5297..5893)
                     /locus_tag="AC158Q_00965"
     CDS             complement(5297..5893)
                     /locus_tag="AC158Q_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3338367.1"
                     /translation="MTPANRLKQAFRILVLAASPLVVLAAAPGAETAAVQATEREVIP
                     GAETMTPAERDAYRRRMEAAATPEEKAKIRAQYAGAAQRPAAARPLIGNPERGATLHR
                     GCFGCHGIERYVAPVTRATASFFDSVLRASGLSDLPPSEPARFKGRIRSLDALRDAVQ
                     RRNDFLNPKMTPQEIEDVVAYLNSTYYKFPELAARAPQ"
     gene            complement(6089..7579)
                     /locus_tag="AC158Q_00970"
     CDS             complement(6089..7579)
                     /locus_tag="AC158Q_00970"
                     /inference="COORDINATES: protein motif:HMM:NF012432.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HIR3338368.1"
                     /translation="MGDSPPPSSQVSGAMPVAPRTGALDINEVFRGGGRSVEFLDEIN
                     KASIVMLDETGIVPHPVAAAIARGIMTVIELDRGTQPRRSADYLDYEPRLAAVAGADA
                     SRLHTGRSRQDIASAIARMNLRDGLLQELEALSAAREKLLAVAGRHTETIIPAYTHGV
                     QAQPTTFAHYLHAFAAALGREVERLQQAYARVNRNPLGAAALATSSFPLDRPRLAALL
                     GFEGLVENAYDANHLAPVDSALEVAGALAIAAVQMGQLAQDIHAQYAGPEPWFLLAEG
                     ELTGVSSIMPQKRNPAALEQLRAQASIMLGEMHTVFLVAHNNRTGMFDYRMYHPVPHE
                     RPLQVFRLFRQVVNGIVVNRERALAEVRADYSTTTEIADALAQRANVPFRAGHHFASK
                     LTDYGRGNGLKLQAIPYAEAARLYQEQTKQPFPLSEAGFAEVISAEYMVHGRKGLGGP
                     QPAEVKRMLSAECDKVAAEFAWLEARAGHLASAGAALERAFAALAS"
     gene            complement(7612..>7800)
                     /locus_tag="AC158Q_00975"
     CDS             complement(7612..>7800)
                     /locus_tag="AC158Q_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3338369.1"
                     /translation="ELAQDDGGKLVIREKLTQQDIASRIGASREMVSKILNDLATGGY
                     LSIARKRITLHKTPPRHW"
BASE COUNT         1206 a         2526 c         2690 g         1378 t
ORIGIN      
        1 tcctggagac cccctcggcc gagcgcctgg cgaccggctt cgtctttacc gaaggcccgc
       61 tgtggcatcc cggcggattt tattacttcg tggacatccg ccagaacaag ctgtaccgca
      121 tcacgccggg cgagaaggcg gaagtcgtcc gggaaaccgc cggcggcaac ggcacgacct
      181 tcgacctcaa gggcaacctg atcctgtgcg aaggcgacgg ccggcgcgtc acgcgcatgg
      241 gcgccgacgg caggatcgag acggtggccg acaactacaa gggcgggcgt ttcaaccgcc
      301 ccaacgacgt ggtgtgccgc tcggacggca gcctgtattt caccgacccc gacaagcgcg
      361 tgccctacgg cgagcgccag ataccggctc ccgccggcga gaacaacctg tgggacggcg
      421 cgcgcgtcta ccgcgtggcg gccgacggcg gcttgagcgt gctcgcccac tgcgagtatc
      481 ccaacgggct cgcgctgtcg ccggacgagc gcacgctcta cgtcgcgaac acccgctcga
      541 cgatgtacgt ccacgcgatc gagctcgacg cggccggcgt catggcgcgg cggcgcatct
      601 tcgccgacat gaacgagggc gcggcgcccg gcattcccga cgggctgaag gtggacagcc
      661 agggccgcgt ctactgcacc ggtccgggcg gcatctgggt gttcacgccg gacggcaagc
      721 gcgtcggcat catccggttc cccgagcagg cggtgaactt cgcattcggc ggcccggacc
      781 tgaagacgct gttctgctgc gcccacacgt ccgtctacac gctgcgggtc aaggtggcgg
      841 ggaacccgca tccctggtac cggctgagga agcagggatg agggatgagg gatgagggat
      901 gagggcggag cgattaaggc aaatggatca ggttttactc atcacccatc acccatcacg
      961 gattttcagg gcaggccgtt cacgcgcgcg tagatgaaca gcgcggcgac cagcgcgagc
     1021 tcggcggccc agatccatcc catttttcgt tgcagcagca ccagcagcgc gcacttgaca
     1081 acgatggcca cgagcccgct cgcggcgaag gtaaggccga ttacccgccc gtagctgtcg
     1141 acccgcgtga aaaagaagaa atagacgcct gccgccagca gacccagccc ggccagggcg
     1201 agcggggtaa tccagtggag tgctttcgcg cgcaagaagc gtagtttacc ggccctgcgg
     1261 tcgagggacg agggaggagg gatgagggag gagggatgat caccggctcc gccggtgttt
     1321 ctcgtcaccc gtcacccgtc actcgtcact cgtcactgta tggtcagcgt ggccgtcgcg
     1381 ctgcccgtgt tctgcgcggc gtcgtccgca gtgattcgga gctcgtagga tccgggcgtg
     1441 gggttgttcc aggcgtacga cacctgcccg ttcatggaag aagtcttcag gacgccgccg
     1501 atgtagagct gcatgccggt cacgccgacg ttgtccgtgg ccgaggcgga aatcgtgagc
     1561 gacgtggtcg tggtggccac tgcgctgttg atcgtgacca tgggcgggat cgtgtcggcg
     1621 gtcgcggcgg gaactaccgc cgacaggtcg aactcgaacg cgccgatatc ggccgcggct
     1681 ccggacggcc ggaacacctt gagatagtcg tccgggacca cgctgacgag attcgcgccg
     1741 gcgtcgatga ccgggctgcc cgtcgtgacc gcgtagcccg atgccgaggc gccgggcgcg
     1801 acgaaccccg gattggcgct gaggctgtgc gcgtccatcc cggtggcggc cacccagccc
     1861 gcaagattct ggtacgtggc gctgtagcgg aggttgatcg cgccggcggg ccggaagaag
     1921 ttgttgtagt cgctcgcgtt gccggcgatg gtggtgccgt tggcgggcac gatgtagagg
     1981 tcgtacgcgc tgctgccgtg gctgacgata ttgttctgga ccgagttgtt cgtcagcgtg
     2041 gacgagccgc gcggcatgcc gccgacctcg atgccggcgc gctcgctggt gccgtagatc
     2101 gtgttgttgt atacccgcgt gttgttggcg gcggagagat agatcccgcg cttgcggttg
     2161 ttcgccacca cgttgttgta gatgagcccg ttgttgctta cctcgaagaa gatcgccccc
     2221 tcggacgggc cgttgtcgtg cacgtagttg ttgcggatga cgatcggcca gctgccgttg
     2281 gcgtagtcga accagatccc ggcgccgttg ttgttggcga tctcgctgtt ctcgatggtg
     2341 ccgtaggcct tggcggcgcc cttgaacccg ccggcgtgcc agttcacgtt gaagttgcgg
     2401 gagttgttgt gcatcagcgt gacgctggtc acccagaagt tcgtgcttcc cgcggcgttg
     2461 atgccggaat tgccgttgtt gctgatgacg cagttgtgcg cgtgcgcgcc gtcctgcagg
     2521 tagcccatgc tgaggccggc aaaatccacg tactggatgt cgcagttctc gatcgaggag
     2581 tgggagctca actccaccgc cgcgccctgc tgcgcgaacg ccgagaggct cgaatggcgg
     2641 aaccggaagc ccttcaggtg gatgtggggc tggtgcatga tgaacaggcg cctgcgcacg
     2701 ctcgcctcca tcacgcgccg gttggggtcg gagccgtccg cgaggcgcac gtacagcgct
     2761 gccgacgcgg ggtcgtagta gaagctgccc gccgtcatgc tcgcgcggcc ggtcccgacc
     2821 gggctgggat actcccagga ggtgtagaac tggctcggca tgccgatctg ctgcagcgat
     2881 ttcttcggct tggcattgaa gtccgcgaac acctgctggc tgttgtgggg ccagccggtc
     2941 ttcttccaga ccacggtgcc gtctctgacc cagccggtga cgacatccga tcccttgatg
     3001 acggggattt ccccgggata ggcggagacg ggaaccggct tgtccgcggt gccgccccgc
     3061 gtggcctcga cctgctcgcg gtaggtgccg ccgcgcacca gcacggtatc gccggggacg
     3121 acggcgttca tcccgcgctg tatggtgcgg aacggctgcg tgacggagga gccgctgttg
     3181 ccgtcattgc cgttcgtcgc gacgtagtag gtcgcggccc aggccggctg cgccaggacg
     3241 gacgccaggc acagcagcgg agcacgccgc aaccgcgccg caagatagtg aagcgctgca
     3301 ctgaagctac gagtattcat cgcggttccc ccgtgaaatc atgagccctg tcgggccctc
     3361 acgccggaac tgcggcacta cgctcgtgcg tcaatccgcg gcggatggct cccgcgcgcc
     3421 gcgatcgacg tagttatcgt tgttgctgct ccaacccgaa acggatcgtc ccctgtcgtg
     3481 gcgtaacacc acagaccggc aatttccacc ttgcggtgga cttgcctcga ccaaacgtgc
     3541 tggtaagtgg aaaactgccc gttacttttc ttggcgacac atcgccgcga attccgcggc
     3601 tgcgataccg ccggcgggca ggccggcgcc ttgatcccgg ttcgtatcgt atgcccgcgg
     3661 tgaccgcaga aagaggaacg atgacacgaa tctccatgac cgtctgctgg cggctcccgg
     3721 cgtgttgcgc gggagataca tcgctcgcgc ccgcggcgaa cctgttggcc acgtttcgcg
     3781 ggcgctgttg ccgtgaggat ggggtccggc ccgggagcag ctggcccgcg gccggccgcc
     3841 ggatccgcgc gcggcgcgaa ccgacgccat ttggcgacac ccggggcggc gagcgtgagt
     3901 tcatcggtga aaactagcgc gccgtgcgcg agtccaaccc caggaatccc cccggaggtt
     3961 tacatggacg gacaggaaag ccgcacgaaa gtggtcatcc tcaccggcag ctaccgcatc
     4021 aagggctata tcgacctgct gccgggcgcg cgggtgacgg attacatact gggcgcgaag
     4081 aacttcatcg tggtgacgga agccgagatc tggtccatcg gcggcaacgg cctggtcctc
     4141 agcgcgcaat tcgtcgacgt gagccgcgac cggatcgagg tgatcaccac caccgaccgg
     4201 cagaagtcgc tggcggcggt cgccgatctg gaacacgtgg cggcagtgag ttaggcgggt
     4261 ctccccggcg acgggtgacc gggcgccccg ggccgcgcgg ggttacccgg cccggctatg
     4321 cccgatccag cacgcagccg acgaaggaca gtccctgcgc cgtgacccac ccggcgtcga
     4381 tctcggcgat gcgccgggtc tccggattac ccgtattgat caggcccgtt tccttgccct
     4441 cgcgtcccca gatctcggtc cggacttcga accggtcctg ggagggcgtc gtggtgaagg
     4501 aaagccgcga agcgctggcc gggtccagcg ggtcgtcccg attgatgatg cgaaaggaaa
     4561 gatgcgcgct cgggtaggcc tccgtgccga cgcgcaggga cacgtccgct tcatgcccgt
     4621 ggcctttcaa ttcctggctc agcgcttcca gcgcgggcgc gatcaccttc tcgatggtgt
     4681 ccaccgccag ggcctggaac acctggtggc ctttttcctc ggcctcttcg acgtccttct
     4741 cctgccgccg tttcgcgccg tgggccttca acagcctgct caactcgtcc ttcatggcgg
     4801 cccctgggtt tgactgcgac cgggcattat ccggcatatc ggtactggat gcccttgaga
     4861 cagggtttat atcagcgagt tgccttgtta tggggcccta gatgaggatt ccggcccctg
     4921 gcgctgacgt gccagcggct tccttttccg cgcccgccgc caccttgggg gcacggatcc
     4981 ttgatattga tcaaggtgcc cggcgcaaat gagcctagat tggatagcgc ttgacctcgg
     5041 aaggagggcg tcatgagcat gattaccgcg atgattctgt ttgccctgct tgccacgatc
     5101 ggttcgctgg cagccggcgt cacgtcgatg gccacgaatg ccgaagtcgg ccatctgcgc
     5161 agcgagcaat ggatgggctg gcgggtcgct ttccaggcag tggcgctgct gttcatcctg
     5221 atcgcgctgc tgacctagcc gcgacccggg ccgcggaaac cgcaacggcg acaagcgccg
     5281 gcgcggcgtc cgcacactat tgcggcgcgc gtgcggccag ttccgggaac ttgtagtacg
     5341 tcgagttgag atacgccacg acgtcttcga tttcctgggg cgtcatcttc ggattgagaa
     5401 aatcattgcg ccgctgcacg gcgtcgcgca gcgcgtcgag cgaccggatc ctgcccttga
     5461 accgggcggg ctccgagggc ggcaggtccg acagcccgct cgccctcaac acggaatcga
     5521 aaaaagaggc ggtcgcgcgc gtgacgggag cgacataccg ttcgatgcca tggcagccga
     5581 agcagccccg gtgcagcgtg gccccccttt caggatttcc gatgagcggg cgggcagccg
     5641 cgggcctttg cgccgcgccg gcatactggg cgcggatctt cgctttttcc tcgggggtgg
     5701 cggcggcctc catgcggcga cggtaggcgt cgcgctcggc gggcgtcatc gtctccgcgc
     5761 ccgggatgac ctcccgttcg gttgcctgga cggccgcagt ctcggcgccc ggcgctgccg
     5821 caagcactac cagcgggctc gcggccagca ccaaaatcct gaatgcctgc ttcaaccggt
     5881 tggctggcgt catggttcac ctcgccgctg tctccgggct cgcgtccgat cggggccggg
     5941 tcgcgacttc atcggcgaaa cggactcgac gcgccttgcc cactcgtgga aaaagtatgg
     6001 gttccgggcg ctgcaggcgt gttgatatag gtcaaacgac cgcaacgtcc ggcgggcgct
     6061 tgccggggcg gcgggagggg cgccgcgttc agctggccag tgcggcgaag gcccgctcga
     6121 gcgcggcgcc ggcgctcgcg agatgtccgg cgcgggcctc gagccacgcg aattccgcgg
     6181 cgaccttgtc acactcggcg gacagcatgc gcttcacctc ggcgggttgc gggccgccga
     6241 gccccttgcg gccgtgcacc atgtattccg cgctgatcac ttccgcgaag ccggcctcgg
     6301 agagcgggaa cggctgtttc gtctgctcct ggtatagccg ggcggcctcc gcgtacggga
     6361 tagcctgcag cttgagcccg ttcccgcggc cgtagtcggt cagcttcgac gcgaaatggt
     6421 ggccggcgcg gaacggcacg ttggcccgct gcgcgagcgc atcggcgatc tcggtcgtcg
     6481 tggaatagtc cgcccgcact tcggcgagcg cgcgctcccg gttgaccacg atgccgttca
     6541 cgacctgccg gaacagcctg aacacctgca gcggccgctc gtgcgggacg gggtggtaca
     6601 tgcggtagtc gaacatgccg gtgcggttgt tgtgggcgac gaggaacacg gtgtgcatct
     6661 cgcccagcat gatgctcgcc tgcgcccgca actgctcgag cgcggcggga ttccttttct
     6721 gcggcatgat gctggagacg cccgtcaatt cgccctccgc cagcaggaac cacggctcgg
     6781 gcccggcgta ctgcgcgtgg atgtcctgcg cgagctgtcc catctggacc gcggcgatcg
     6841 ccagcgcgcc cgcgacctcg agcgcgctgt ccaccggcgc gaggtggttc gcgtcgtagg
     6901 cattctcgac gagcccctcg aagcccagca gcgccgcgag acgcgggcgg tcgagcggaa
     6961 agctcgacgt ggcgagggcg gcggcgccca ggggattgcg gttcacgcgc gcgtacgcct
     7021 gctgcagccg ctcgacctca cgccccagcg cggcggcaaa cgcgtgcagg taatgcgcga
     7081 atgtggtggg ctgcgcctgc acgccgtgcg tgtaggcggg aatgatggtc tccgtgtgcc
     7141 ggcccgccac ggcgagcagc ttctcgcggg cggccgacag cgcttcgagc tcctgcagca
     7201 gcccgtcgcg caggttcatg cgcgcgatgg cggaggcgat gtcctggcgg cttctgccgg
     7261 tgtgcaggcg ggaggcgtcc gcgcccgcca cggcggcgag cctcggctcg tagtccaggt
     7321 agtcggcgga gcgccgcggc tgcgtcccgc ggtcgagctc gatgaccgtc atgatgcccc
     7381 tcgcgatcgc ggccgcgacc gggtggggca cgatcccggt ctcgtcgagc atgacgatgg
     7441 acgccttgtt gatttcgtcg aggaactcga ccgagcgacc gccgccgcgg aacacctcgt
     7501 tgatgtccag cgcgccggtg cgcggcgcga caggcattgc gccggaaacc tgcgaactcg
     7561 ggggagggga gtcgcccatc gggcatttcc tttcgtgtgt ctgcaaacgc cttaccagtg
     7621 ccgcggcggc gtcttatgta gggtaatgcg cttccgggcg atgctgagat aaccccccgt
     7681 cgcgaggtcg ttgagtatct tgctgaccat ttcgcgcgac gcacctatcc ggctggcgat
     7741 gtcctgctgg gtgagcttct cccggatgac gagcttgccg ccatcgtcct gcgccagttc
//
LOCUS       DBNGSG010000042         3900 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_691772, whole genome shotgun sequence.
ACCESSION   DBNGSG010000042 DBNGSG010000000
VERSION     DBNGSG010000042.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3900)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_691772"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="AC158Q_00980"
     CDS             <1..438
                     /locus_tag="AC158Q_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR3338370.1"
                     /translation="TPDERQIINHHIEVTIQMLEALPWPRHLKNVPEYAGGHHERMDG
                     KGYPRGLTREQMSIQARCMGIADIFEALTAKDRPYKKGKTLTESLTILGKFKLNGHVD
                     PDLFDVFMWEKVFEKYARQFLDPDQIDEVDVTRIPGYVPPPSH"
     gene            complement(451..762)
                     /locus_tag="AC158Q_00985"
     CDS             complement(451..762)
                     /locus_tag="AC158Q_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338371.1"
                     /translation="MAWFGSVSGSQVDNFAKSLVQELARHYPPAYEQGEGKADRKVFS
                     VLDSLFGKARDFRNLHNLGVYKKARLANTFRWEMQELGYSAKFTEFATEGLVVSMTQK
                     G"
     gene            884..3892
                     /locus_tag="AC158Q_00990"
     CDS             884..3892
                     /locus_tag="AC158Q_00990"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013713.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermine/spermidine synthase domain-containing
                     protein"
                     /protein_id="HIR3338372.1"
                     /translation="MSGRQSADFVRGNPANCINSMTTPFARAWLLAIFTVSGFSGLIY
                     ESIWSHYLKLFLGHAAYAQALVLAIFMGGMALGAWICSLKSDRWPHVLLIYAGVESVL
                     GLLAFLFHPVFLASTEFGYDTAMPALSGSPLAVSAFKWTLAALLILPQSTLLGMTFPL
                     MSAGILRAHPDRQGETISMLYFTNSLGAAAGVLASGFFLVRAVGLPGTMLTAGLLNCG
                     IALAVWLIGRNSPQSVMPRTDERSPNAARPARLLLMVSLLTGAASFMYEVGWIRMLSM
                     VLSSSTHAFELMLSAFILGLAFGGLWLRRRIDAIRFPETFLGGVQIAMGLLALATLLV
                     YERTFELMHILLDTVARNGPGYTAFNFGSHLIALLIMFPAAFCAGMTLPLITSTLLRS
                     GSGERAIGAVYAANTVGAIVGVFVAVHFAMPILGLKGLITLGAAIDIALGLVLLWRYA
                     LTPIALRGAAALGACAIVATLAWVELDSLKMASGVYRHGGVTPQGQAEVLFHRDGKTA
                     TVNLLRAGGKLSITTNGKSDAMIDLSPQAPASDDEPVMILTGALPLALHPDARTAAVI
                     GIGSGISSHVLLSSSTLRAVDTIEIEPAMVEAARGFGSRNDLVFSDPRSRIYIEDAKI
                     HFSTHGKRYDIIVSEPSNPWVSGVASLFTDEFYQRMTRHLEKDGLFVQWIQLYEIDTR
                     LVASIIKALARNFSHYEVYTADDHNLIIAARRDLSLPPPSGAVFHQARLAAELERLDV
                     RNAADLDVFRLGSERVLQPFFNRYPIAPNSDFFPVLDQNAAKARFLGAWAREIIDLGN
                     MPIPAIEFLGGVPRRATPVAPRTWLRRSELTHAAQQGRDFLLSGATHHLEGLPVPLRS
                     DFALTRLALRECHPNLHGVSIDQLFAVAGALIPQLPRDAGNSVWEALKASRCTRGPQA
                     RLITLFSAIDARNGEAMAHIAESELRSGDPAMKRRRDFFLAAAIVGRLSNGDRARAAA
                     LWNEFADGMTSERGNILPELLQGHIFAPTH"
BASE COUNT          707 a         1212 c         1143 g          838 t
ORIGIN      
        1 accccggatg agcggcagat catcaaccac cacatcgaag tcacgatcca gatgctggag
       61 gccctgccgt ggcccaggca cctgaagaac gtgcccgagt atgccggcgg ccaccatgag
      121 cgcatggatg gcaagggcta cccccgggga ctcacccgcg agcagatgtc gatccaggcg
      181 cgctgcatgg gcattgccga catctttgag gcactgaccg ccaaggaccg cccgtacaag
      241 aaaggcaaga cgctcacgga gtcgctcacc atactgggaa agttcaaact gaacgggcac
      301 gtcgacccgg atcttttcga cgtcttcatg tgggaaaagg tcttcgagaa gtacgcccgg
      361 cagttcctcg accccgacca gatcgacgag gtggacgtga ccaggatccc cggctacgtt
      421 ccaccgccgt cgcattgacg gcgaacggaa tcagcccttc tgcgtcatgc tcacgaccag
      481 gccttcggtt gcgaactcgg tgaatttggc gctgtagccc aattcctgca tctcccagcg
      541 aaacgtgttc gccaatcgcg cttttttgta gacgccgaga ttgtgcagat tcctgaaatc
      601 ccgcgccttg ccgaatagac tgtccagcac ggagaacacc ttccggtccg cttttccttc
      661 cccctgctcg tacgcagggg ggtaatgccg cgcaagctcc tgcacgagcg acttggcgaa
      721 gttgtccacc tggctgccgg aaacagaacc aaaccatgcc atcgttcatt cccctcactg
      781 cctttccaga tcacatcttg cattgcaggc agtgcatcgt caatcgcccg cacggggatt
      841 tttccaggga ttcaatgaat agacatcacc cgggacggca tgggtgtcgg gccgccagtc
      901 ggctgacttt gtccgcggca atcctgcaaa ctgcatcaac tcgatgacta cgccctttgc
      961 ccgtgcctgg ctgctggcga tattcacggt gtccggcttt tccgggctga tctatgaatc
     1021 catctggagc cattatctca agctctttct cggtcacgcg gcgtacgcgc aggccctggt
     1081 ccttgcgata ttcatgggcg gcatggcgct cggcgcctgg atctgcagcc tgaaatctga
     1141 tcgatggccc catgtgctcc tgatctacgc cggagtggaa tcggtgctgg gattgttggc
     1201 attcctgttt cacccggttt tcctggcctc gaccgaattc ggctatgaca cagcgatgcc
     1261 ggcgctgtcc ggctcgccac tcgcagtcag cgcgttcaag tggacgcttg ccgccctgct
     1321 cattctcccc cagtcgacgc tgctcggaat gacgtttccg ctgatgagcg cgggcatcct
     1381 gcgcgcccat ccagatcgcc agggggaaac aatctcgatg ctgtacttca cgaacagcct
     1441 cggtgcggcc gccggcgttc ttgccagcgg attctttctc gttcgagccg tgggtctgcc
     1501 cggcacgatg cttaccgcgg gcctgctcaa ttgcggtatc gctttggcgg tgtggctgat
     1561 tggaagaaat tccccgcaat ccgtaatgcc gcggactgat gaacggtcac cgaatgcagc
     1621 gcgtcccgcg cgcttgctgc tgatggtatc gcttctcacc ggcgcggcat ccttcatgta
     1681 cgaggtggga tggatccgga tgctcagcat ggtgctgagc agttcgacgc acgccttcga
     1741 actgatgttg agcgcgttca ttctcggcct tgctttcggg ggcctgtggt tgaggcggcg
     1801 catagacgca attcgctttc ctgaaacatt cctgggtggc gtgcagatcg cgatgggcct
     1861 gctcgctctc gcgacgctcc tggtctacga gcggacattc gaattgatgc atatcctgct
     1921 ggataccgtc gcgcgcaacg ggccgggata taccgcattc aactttggca gccacctcat
     1981 agcgctgctc atcatgttcc cggcggcgtt ttgcgccggc atgactctgc cgctgatcac
     2041 ctcaaccctg ctgcgcagcg gcagcggcga gcgggcgata ggagccgtct acgcggcgaa
     2101 tacggtcggc gccatcgtgg gggtatttgt cgcggtgcac tttgccatgc ccattctggg
     2161 cctcaaggga ctgatcacgc tgggcgcggc tatagacatc gcattagggc ttgtcctttt
     2221 gtggcgctac gcgctcacgc ccattgcgct gcgcggcgcc gctgcgctgg gagcctgtgc
     2281 catcgtggcg acactggcct gggtggaact cgattcgttg aagatggcct ccggcgtcta
     2341 ccggcacggg ggggtgaccc cgcagggtca ggcggaggtg ctttttcatc gtgatggcaa
     2401 gacggccacc gtcaatctcc tgcgagccgg cggaaaactg tctatcacca ccaacggcaa
     2461 gtcggacgcc atgattgatc tgagcccgca agcccccgcc tccgacgatg aacccgtcat
     2521 gattcttacc ggggccttgc cgcttgctct tcacccggac gcgcgcacgg cagccgtaat
     2581 cggcatcgga tcgggcattt ccagccatgt attgctttcg tcaagcactc tgcgggcagt
     2641 cgataccatc gaaatcgagc cggccatggt ggaggctgcg cggggcttcg ggtcccgcaa
     2701 tgacctggtg ttcagcgatc cacgtagtcg tatttacatc gaggacgcga aaatccactt
     2761 ctccacccat ggcaagcgct acgacatcat tgtgtccgaa ccctccaatc cctgggtcag
     2821 cggtgtcgcg agcctgttca cagacgagtt ctaccaacga atgacccgtc acctggaaaa
     2881 agatggatta ttcgtgcagt ggattcagct ctacgagatc gatacccggc tcgtcgcctc
     2941 gatcatcaag gcgctggcgc gcaatttttc ccattacgag gtctacaccg ctgacgatca
     3001 caatttgatc atcgctgccc gtcgggatct gtcattgccg ccaccgtcgg gcgccgtgtt
     3061 ccatcaggcc cgccttgcgg ccgaacttga gcgactcgat gtccgtaatg cggccgatct
     3121 ggatgtattc aggctgggtt cggagcgtgt tctgcagccg ttcttcaatc gttatccgat
     3181 tgcaccgaac tcggattttt ttccggtact tgatcaaaat gcggcaaaag cccgttttct
     3241 cggcgcatgg gcgcgggaaa tcatcgatct cgggaatatg cctattccgg cgatcgaatt
     3301 tctcggtggc gtgccacggc gcgccacgcc ggttgcgcca cgaacctggc tgcggcgcag
     3361 cgagctcact cacgcggcgc agcaaggaag ggacttcctc ttaagcggag ctacgcatca
     3421 tctcgaagga ctgcccgtgc ccttgagaag cgatttcgcg ctgacgcgtc ttgctctgcg
     3481 tgaatgccat ccgaacctgc acggcgtttc catcgatcag ttgtttgcgg tcgcgggtgc
     3541 attgatcccg cagctgccgc gggacgcggg caacagtgtg tgggaggcgc tcaaggcatc
     3601 acgctgcacg cggggtccgc aggcaaggtt gatcacgctc ttctcggcca tcgatgcgcg
     3661 caacggtgaa gccatggcgc atatcgccga gtcggaactc aggagcgggg accccgctat
     3721 gaagcgacgt cgcgactttt ttctggcggc ggccattgtc ggccggcttt cgaacgggga
     3781 ccgcgcccgc gcagccgcgt tgtggaatga atttgcggac ggcatgacca gcgagcgcgg
     3841 aaatattctc cccgagctcc tgcaagggca tatatttgcc cctacacact gaggcaaccc
//
LOCUS       DBNGSG010000043         4765 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2560114, whole genome shotgun sequence.
ACCESSION   DBNGSG010000043 DBNGSG010000000
VERSION     DBNGSG010000043.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2560114"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1904
                     /locus_tag="AC158Q_00995"
     CDS             <1..1904
                     /locus_tag="AC158Q_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR3338373.1"
                     /translation="VRFGQEIAGVMELGMIGRGEHAEIITFVGKTVYSELSGNLIDLC
                     PVGALTSKPFRYTARTWELTRKPSVSPHCGLGSNLTVQVKQNRVMRVLPRENEAVNEC
                     WLSDKDRFSYEGLNSDQRLAKPMLKQGGAWKEVEWQVALDYVANELKRIKDAHGAASI
                     GMLATPQQTVEELYLLGKLAREFSSGNVDFRLRQSDFSADGGLAGAPWLGMAVAEVSQ
                     LDRVLVVGSTLRKDHPLIAHRLRQAAKRATQLNFINPFDDELLMRVANKAVVAPADMP
                     GLLAQVLRSAAEQKGAPVPEAVRGVTVSDAAQRIAASLAGGKNAAIFLGNLAQHHPQA
                     AQLHALAQALAEATGARFGFLGEAANSVGGYLAGAVPAAGGKNAVQMLKGPLKAYLLL
                     GVEAELDTHDPKQAVAAMKSAELVVAMSPYQHHAVEYAHVLLPIAPFTETAGTFVSTE
                     GRVQGFSGVVRPLGETRPAWKVLRVLGNLLGLAGFDHDSADDVKREALGNGEVGGRLG
                     NQWKQAKIGAVQPAQGGGLQRIAEVPVYAADAIVRRARALQLTHDAVPAVAWMNRALF
                     EKLGLREGDGVRVRQGGGEAVLAAGVDDKLPAECMRIAAARPETAALGALFGTITAER
                     VPARGKVAV"
     gene            1952..3028
                     /gene="nuoH"
                     /locus_tag="AC158Q_01000"
     CDS             1952..3028
                     /gene="nuoH"
                     /locus_tag="AC158Q_01000"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HIR3338374.1"
                     /translation="MLAAIVVWIVGQETAPAVLYVIKILLLILCIVVPLMLCVAYLTL
                     WERKVIGWMQIRIGPNRVTFFGIPWLGGLAQPIADGLKLLVKEIIVPAGANKLMFVLA
                     PIMAITPALAAWAVVPFSPELILSDIDAGLLYVMAITSMGVYGIIIAGWASNSKYAFL
                     GAMRSAAQMVSYELAMGFALVCVLMAAQSLNLGEIVKAQDGAWGLANWFWIPLFPIFL
                     VYIIAGVAETNRAPFDVAEGESEIVAGFHVEYSGMAFAVFFLAEYANMILISTLASIM
                     FLGGWLSPFPQSWGIIGAPGFWWLIVKLFLVVSSFLWFRATFPRYRYDQLMRLGWKVF
                     LPLTIAYIVVLGAWMQTPLWIWWK"
     gene            3028..3519
                     /gene="nuoI"
                     /locus_tag="AC158Q_01005"
     CDS             3028..3519
                     /gene="nuoI"
                     /locus_tag="AC158Q_01005"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HIR3338375.1"
                     /translation="MVERVRDFLKTFLLVELLQGMMLTGRYLFARKITVQFPEEKTPQ
                     SPRFRGLHALRRYPNGEERCIACKLCEAVCPALAITIESEQRDDGTRRTTRYDIDLTK
                     CIFCGFCEESCPVDSIVETRILEYHGEVRGDLIYTKPMLLAVGDRYEAEIAKDREDDA
                     AFR"
     gene            3613..4227
                     /locus_tag="AC158Q_01010"
     CDS             3613..4227
                     /locus_tag="AC158Q_01010"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HIR3338376.1"
                     /translation="MSFPEIVFYFFAAILVLSALGVITARNPVHAALLLVLAFCTSAG
                     LWMQLQAEFLAITLVLVYVGAVMVLFLFVVMMLDINLTRLREGFWNYLPLGGLIALIL
                     VAEMWFTLEYLSGFAEAPAPPPLPAGYSNTKELGRLIYTEYVYPFEIAAVILLVAIVS
                     AIAITMRRRKDSKYVDPAKQVAVRRADRVRIVQMPAEKKEGGGG"
     gene            4224..4529
                     /gene="nuoK"
                     /locus_tag="AC158Q_01015"
     CDS             4224..4529
                     /gene="nuoK"
                     /locus_tag="AC158Q_01015"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003261941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HIR3338377.1"
                     /translation="MISLSHYLVLGAVLFAIAVIGIFLNRKNMIVLLMAIELMLLAVN
                     MNFVAFSHFLDDPSGQVFVFFILTVAAAESAIGLAILVALFRNLNTINVEDLDSLKG"
BASE COUNT          780 a         1462 c         1607 g          916 t
ORIGIN      
        1 gcgtgcgctt cggccaggag atcgcgggcg tgatggagct cgggatgatc ggtcgcggcg
       61 agcacgcgga gatcatcacg ttcgtcggca agaccgtgta ctccgagctc tccggcaact
      121 tgatagacct ctgcccggtc ggggcgctca cttccaagcc tttccgttac accgcccgta
      181 cctgggagct gacgcgcaag ccctcggtct ccccgcactg cggcctgggg tccaacctga
      241 cggtccaggt gaagcagaac cgcgtaatga gggtgctgcc gcgcgagaac gaggcggtca
      301 acgaatgctg gctgtcggac aaggaccgct tttcctacga aggcctcaat tccgatcagc
      361 ggcttgcgaa acccatgctg aagcagggcg gcgcctggaa agaagtggag tggcaggttg
      421 ccctcgacta cgtggcgaac gagctgaagc gcatcaagga tgcccatggt gccgcgagca
      481 ttggcatgct ggcgacgcct cagcagacgg tggaggagtt gtacctgtta ggaaaactcg
      541 ctcgcgagtt ttcctcgggt aacgtcgatt tccgtctgcg ccagtccgac ttcagcgccg
      601 acggcgggct tgccggtgcg ccgtggctcg gcatggcggt ggcggaggtg agccagcttg
      661 atcgcgtgct ggtggtcggc tccacgctga ggaaggatca cccgctgatc gcccatcgtt
      721 tacggcaggc cgcgaagcgc gccacccagc tcaatttcat caatccgttc gacgacgagc
      781 tgctgatgcg ggtggcgaac aaggcggtcg tcgcgcccgc ggacatgccc ggcctgctcg
      841 cgcaggtgct caggtccgcc gcggagcaga agggcgctcc ggttcctgag gccgtgcgcg
      901 gcgtaacggt atccgatgcg gcgcagcgca tcgcggcgag cctcgccggc ggcaagaacg
      961 cggcgatttt tctcggtaac ctcgcgcagc accacccgca ggccgcgcag ctgcacgcgc
     1021 tggcgcaggc actggcagag gcgacgggtg cccgtttcgg cttcctcggg gaagccgcca
     1081 acagtgtcgg cggctacctc gccggcgcgg ttccggccgc gggcggcaag aacgccgtgc
     1141 agatgctgaa agggccgtta aaagcttacc tgctgctggg cgtcgaagcg gagctggaca
     1201 cccatgaccc gaagcaggcg gttgcggcaa tgaaatccgc cgagctggtg gtggcgatga
     1261 gcccgtacca gcaccacgcc gtcgaatatg cccacgtgct gctgccgatc gcgccgttta
     1321 ccgagaccgc cggcaccttc gtcagcaccg agggcagggt gcagggcttc agcggcgtgg
     1381 tgcggccgct gggggaaacc cgccccgcgt ggaaagtgct gcgcgtgctg ggcaacctgc
     1441 tggggctggc cggtttcgat catgacagcg cagacgacgt gaaacgcgag gcgctcggca
     1501 atggcgaggt gggcggcagg ctcggcaatc aatggaagca ggcgaagatc ggcgcggtcc
     1561 agcccgcgca gggcggcggg ctgcagcgca tagccgaagt gccggtctat gcggccgacg
     1621 ccatcgtgcg ccgcgcccgg gcgctgcagc tcacgcatga cgcggttcct gccgttgcct
     1681 ggatgaaccg ggcgctcttt gagaagctgg gactgcgcga aggcgacggc gtgcgggtgc
     1741 gccagggcgg cggcgaggcg gtgcttgcgg ccggcgtcga tgacaaattg cctgcggagt
     1801 gcatgcggat cgcggcagcg cggcccgaga ccgcagcgct gggcgccctg ttcggaacga
     1861 tcacggccga acgagtgccc gcgcgcggga aggtggcggt gtgagcacgg tactgcaacc
     1921 cttcatcagc atcggcaact ggctgtgggg cctgctggct gcgatcgtgg tatggatcgt
     1981 ggggcaggag accgcgccgg cggtgctcta cgtgatcaag atactgctcc tgatcctgtg
     2041 catcgtcgtg ccgctgatgc tgtgcgtggc ctacctcacg ctgtgggagc gcaaggtcat
     2101 cggctggatg cagatccgca tcgggccgaa ccgcgtgacc ttcttcggca taccctggct
     2161 cggcggcctt gcgcagccga tcgcggacgg attgaagctg ctggtgaagg aaatcatcgt
     2221 ccccgccggc gccaacaagc tcatgttcgt cctcgcgccg atcatggcga tcacgccggc
     2281 gcttgccgcg tgggccgtgg tgccgttcag ccccgagctt atcctgtccg acatcgacgc
     2341 cggtttgctc tacgtgatgg ccatcacctc gatgggagtc tacggcatca tcatcgcggg
     2401 ctgggcctcg aattccaagt acgcgttcct cggcgcgatg cgttctgcgg cgcagatggt
     2461 gtcctacgag ctggcgatgg ggtttgcgct ggtgtgcgtg ctgatggcgg cgcaaagcct
     2521 caatctcggc gagatcgtca aggcccagga cggcgcctgg ggcctggcca actggttctg
     2581 gatcccgctg ttcccgattt tcctcgtcta catcatcgcc ggcgtggcgg agaccaatcg
     2641 cgcgccgttc gacgtggccg agggtgagtc cgaaatcgtc gcgggcttcc acgtcgagta
     2701 ctcgggcatg gcgttcgcgg tgttcttcct cgccgagtac gcgaacatga tcctgatctc
     2761 gacgctcgcg tccatcatgt tccttggcgg ctggctctcg ccgtttccgc agtcgtgggg
     2821 aattatcggc gctcccggct tctggtggct catcgtgaag ctgttcctgg tggtgtcgag
     2881 cttcctgtgg tttcgcgcga cgttcccgcg ctaccgctac gaccagctga tgcggctcgg
     2941 ctggaaagtg tttctgccgc tcaccatcgc ctatatcgtg gtgctgggcg cctggatgca
     3001 gactccgctg tggatatggt ggaaataatg gtcgagcgcg tccgcgattt cctgaagacc
     3061 ttcctgctcg tcgagctgct gcagggcatg atgctgaccg gccgctacct attcgcgcgc
     3121 aagatcacgg tgcagtttcc ggaggagaag acgccgcaaa gcccgcgttt tcgcgggctg
     3181 cacgcgctgc ggcgctaccc caacggcgaa gagcgctgca tcgcctgcaa gttgtgcgag
     3241 gcggtgtgcc cggcgctcgc catcaccatc gagtccgagc agcgcgacga cggcacgcgc
     3301 cgcaccacgc gctacgacat cgatctcacc aagtgcatct tctgcggttt ctgcgaggag
     3361 tcgtgcccgg tggactcgat cgtggagacc cgcatcctcg agtaccacgg cgaggtgcgg
     3421 ggggatctca tctataccaa gccgatgctg ctcgcggtcg gggaccgcta cgaagccgag
     3481 atcgcgaagg accgcgagga cgatgcggcc ttcagatgag ccgcaaaggc gcggagccgc
     3541 aagggatttt aacgaaatga atcatgaata ttttcttggc gcctttgcgt ctctgcggct
     3601 gaatctgcct tcatgagctt tccggaaatc gttttctact tcttcgccgc gatcctggtg
     3661 ctctcggcgc tcggcgtgat taccgcgcgc aacccggtgc acgcggcgct gctgctggtg
     3721 ctggcgttct gcacctccgc gggactgtgg atgcagctgc aggccgagtt cctcgccatc
     3781 acgctggtgc tggtctacgt cggcgccgtg atggtgctgt tcctgttcgt ggtgatgatg
     3841 ctcgacatca acctgacccg gctgcgggag gggttctgga actatctgcc gctgggcggc
     3901 ctgatcgcgc tgatactggt ggcggagatg tggttcacgc tcgagtatct ttcgggtttc
     3961 gcggaggcgc cggcgccccc gcccctgccg gcgggctaca gcaacaccaa ggagctcggc
     4021 cggctgatct ataccgaata cgtctacccg ttcgagattg ccgcggtgat cctgctggtt
     4081 gccatcgtct cggcaatcgc gatcaccatg cgccggcgca aggactcgaa gtacgtcgac
     4141 ccggcgaaac aggtcgccgt gcgccgagcg gaccgcgtgc gcatcgtgca aatgccggcg
     4201 gagaagaagg aagggggagg cgggtgatct cgctatcgca ctatctggtg ctgggcgcgg
     4261 tcctgttcgc gatcgcggtc atcggcatct ttctcaaccg gaaaaacatg atcgtgctgc
     4321 tgatggcgat cgagctgatg ctgctcgccg tcaacatgaa cttcgtcgca ttctcgcact
     4381 tcctcgacga cccttcgggg caggtcttcg tgttcttcat cctgaccgtc gccgcggcgg
     4441 agtccgcgat cgggctggcg atcctggtgg cgctgttccg caacctgaat acgatcaatg
     4501 tcgaggattt ggatagcctg aaaggatgaa gcacagtgtt tagttttgaa tttttagtgt
     4561 tgagttaacg gctggccgac gatcgcgtac tgaaaatgat aaaactcaac actcaacact
     4621 caacactcaa aactggcgcc gagcgatgac acggatgcaa ttgctatacc ttatcgtgcc
     4681 gctcgctccc ctcgtcggcg cgatcgtggc cggcctgttc ggccgcgcga tcgggcgcgc
     4741 gggcgcgcac tgggtcacga tcgtg
//
LOCUS       DBNGSG010000044         2193 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2900186, whole genome shotgun sequence.
ACCESSION   DBNGSG010000044 DBNGSG010000000
VERSION     DBNGSG010000044.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2900186"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1977)
                     /locus_tag="AC158Q_01020"
     CDS             complement(<1..1977)
                     /locus_tag="AC158Q_01020"
                     /EC_number="2.3.1.-"
                     /EC_number="6.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0034081 - polyketide synthase complex
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030639 - polyketide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HIR3338378.1"
                     /translation="MRQKRVAIVGMSFRLPGSTRESFWPALLAGKNLVTEVGPERWSQ
                     DTFQHPKRSHPGTSYTFAAGNIGDISGFDAAFFGISPREAAQMDPQQRLLLELTWEAF
                     ENAGIKPSTVRGSACGVYVGVASLDYSLRFTDDLAAVDSSFATGNTASIAANRISYVF
                     DLHGPSLSVDTACSSSLVAFHLACRSIISGECSQAITGGVSLHMHPYGFVVFSKASML
                     SRRGICNVFDAAGDGYVRSEGGALFMLKDYDQALADGDRIIAVVANSAVNSDGRKSGL
                     TVPSARAQAELLKHAYDEAGIAASEIDYIEAHGTGTAVGDPIETHALGEALGQSRDAA
                     TPLLIGSVKSNLGHLETAAGAAGLVKALYCIQHRTVPATIHLNEPNPNIRFEEWNLKV
                     PTEPTPLKGGGKLIIGVNSFGFGGSNAHVILTSPESRPRRRTRQAPSMPLPVVLTGKS
                     AAALKAAARDLALFLDEQKKASLYDVAYTAAFHREWHDQRAVFMAGSTKTLAASLKKF
                     ADDAAPEAEVETGTVLPPPADVAFVYSGNGSQWEGMGRRLIAEEPVFRDAVRAIDEVF
                     RPLAGFSLEDELSGRNGSGRYEYTEIAQPALFAVQVGVTEVLRARGLAPAAVAGHSVG
                     EVAAAWACGALTLEQAVRVIYHRSRLQSTTKGMGQ"
     gene            complement(1991..>2193)
                     /locus_tag="AC158Q_01025"
     CDS             complement(1991..>2193)
                     /locus_tag="AC158Q_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338379.1"
                     /translation="AEDGRLYSFKLGRIHCAGVAVEYGPGRSFRGLDNKQVITMVMEN
                     FLSAGGSGGFRAGRRGACRGAD"
BASE COUNT          340 a          745 c          741 g          367 t
ORIGIN      
        1 ctgccccatg cccttggtcg tgctctgcag ccggctgcgg tgatagatca cgcgcaccgc
       61 ctgctcgagc gtgagcgccc cgcacgccca ggcggcggca acctcgccga cgctgtgccc
      121 ggccaccgcg gcgggcgcca ggccgcgcgc ccgcaatact tcggtcacgc cgacctggac
      181 ggcgaacagc gccggctggg cgatctcggt gtattcgtag cgcccggatc cgttcctgcc
      241 cgagagttcg tcctcgaggg aaaacccggc caacggccgg aacacttcat cgatggcccg
      301 caccgcgtcc ctgaacaccg gctcctcggc gatcaggcgc cgccccatgc cttcccattg
      361 cgagccgttg cccgagtaaa cgaacgcgac atccgccggg ggcggcagca ccgtgccggt
      421 ttccacctcg gcctcgggcg cggcatcgtc cgcgaatttt ttcagcgacg cggcgagcgt
      481 tttcgtggag cccgccatga aaacggcccg ctggtcgtgc cattcgcgat ggaaggcggc
      541 ggtataggcc acgtcgtaga gcgaggcctt cttctgctca tccaggaaca gcgcgagatc
      601 gcgcgcggct gctttcagcg ccgccgcgct tttccccgtg agcacgacgg gcagcggcat
      661 cgagggagcc tgccgggtcc tgcggcgcgg ccggctctcg gggctcgtga ggatgacgtg
      721 ggcgttcgat ccgccgaagc cgaaagagtt gacgccgatg atcagcttgc cgccgccctt
      781 gagcggcgtg ggctcggtgg gaaccttgag gttccattcc tcgaagcgga tgttcgggtt
      841 gggttcgttc agatgtatgg tcgccggcac cgtcctgtgc tgtatgcagt agagcgcctt
      901 caccaggccc gccgcgccgg ccgcggtttc gaggtggccg aggttgctct tgaccgagcc
      961 gatcagcagg ggcgtggcgg catcgcggga ctggcccagc gcttccccca gcgcgtgcgt
     1021 ttcgatcggg tcgcccaccg ccgtgccggt gccgtgcgcc tcgatatagt cgatctcgga
     1081 cgcggcgatc ccggcttcgt cgtaggcgtg cttcagcagc tccgcctggg cccgggcgct
     1141 cggcacggtc aggccggact tgcgcccgtc gctgttgacc gccgaattcg cgacgacggc
     1201 gatgatccgg tccccgtccg ccagcgcctg gtcgtagtcc ttgagcatga acagggcgcc
     1261 gccctccgag cgcacgtaac cgtcgccggc agcgtcgaac acgttgcaga tcccccgcct
     1321 cgacagcatc gacgccttcg aaaagacgac gaacccgtag gggtgcatgt gcaggctcac
     1381 gcccccggtg atggcctgcg agcactcgcc cgagatgatg gagcggcagg ccaggtggaa
     1441 cgcgaccagc gaggacgagc aggcggtgtc caccgacagg ctggggccgt gcaggtcgaa
     1501 tacgtaggag atccggttgg ccgcgatgct ggcggtgttg ccggtggcga acgacgaatc
     1561 gaccgccgcg aggtcgtcgg tgaaccgcag cgagtaatcg aggctggcaa cgccgacgta
     1621 tacgccgcac gcgctgccgc gcaccgtgga aggcttgatg cccgcgttct cgaacgcctc
     1681 ccaggtcagc tcgagcagca ggcgctgttg cgggtccatc tgcgccgctt cccgcggcga
     1741 aatgccgaag aacgccgcgt cgaagccgga aatgtccccg atgttgccgg cggcgaaggt
     1801 atagctggtg ccggggtgcg agcgcttggg gtgctggaaa gtgtcctgcg accagcgctc
     1861 ggggccgacc tcggtgacga gattttttcc cgccagcagg gcgggccaga agctctcgcg
     1921 ggtggaaccg ggcagccgga acgacatgcc cacgatggcg acgcgttttt gtctcatttg
     1981 cctggccggg tcaatcggcg ccgcggcacg cgccgcggcg acccgcccgg aatccgccgc
     2041 tgccccctgc ggacaaaaaa ttctccatca ccatcgtgat gacctgtttg ttatcgagtc
     2101 cccggaagct tcggcccggc ccgtactcaa ctgcaacgcc cgcgcaatga atgcggccta
     2161 gtttgaaact atacaaccgg ccgtcttctg cta
//
LOCUS       DBNGSG010000045         2710 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3239608, whole genome shotgun sequence.
ACCESSION   DBNGSG010000045 DBNGSG010000000
VERSION     DBNGSG010000045.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3239608"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /gene="gshA"
                     /locus_tag="AC158Q_01030"
     CDS             <1..960
                     /gene="gshA"
                     /locus_tag="AC158Q_01030"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HIR3338380.1"
                     /translation="SILRQAGIQVRIGTLLADITGPTEVEIEGGGRLKLEPLERRGNR
                     LAVNGFDPCVVLLNNDLSSGTPEILMNLEQAVLPPLHAGWTVRRKSNHFAAYRQVAEE
                     FAGLLGIDPWFINPYFEKCGKINFQEKKGEECLEGYVAEILDDIRAKYKEYGITQDPF
                     VIVKADAGTYGMGVMTVKDPTEVRELNRKQRNKMAVVKEGLEVREVMVQEGIYTFESV
                     DDAAAEPVVYMIDHFVVGGFYRVHTARGQDQNLNAPGMQFKPLAFAEPCSSPNPDDPG
                     CPPNRFYAYGVIARLALLAAAIELEQTEAALEAGAAPRIAAAS"
     gene            957..1925
                     /gene="gshB"
                     /locus_tag="AC158Q_01035"
     CDS             957..1925
                     /gene="gshB"
                     /locus_tag="AC158Q_01035"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HIR3338381.1"
                     /translation="MKLAFVVDPLDSFKIQKDTTFAIMREAAGRGHALFAMEQDDLSW
                     HGGTVTGNSRELHLTGEKPRWYRAGEPAATPLQAFDAVLMRKDPPFDMEYVYSTYLLE
                     LAELHGARVINRPRAIRDWNEKMGITRFPQFAPPTMVTRRDRLIHEFLAEHRDVILKP
                     LDGMGGASVFRVRQDDPNVNVIIETVTHHGRRTIMAQRYLPEIRDGDKRVLLIGGKPV
                     PHCLARVPRPGETRGNLAAGATGVARELTTRDREIAEALGPVLHKEGLLLVGLDVIGD
                     CLTEVNVTSPTCFQEITEQSGFNVAGMMLDALEREIGRPAARKMKQ"
     gene            2046..2468
                     /locus_tag="AC158Q_01040"
     CDS             2046..2468
                     /locus_tag="AC158Q_01040"
                     /inference="COORDINATES: protein motif:HMM:NF015567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIR3338382.1"
                     /translation="MVGILIISHGTLGEALIHCASHVLNKRPPRLKQLGITAQDDPLQ
                     LLPQARKLVKDLDEGDGVLILSDMYGGSPSNIAAKLLVPGKIEGVAGVNLPMLIRVLT
                     YRDKSLQTILTKAVSGGCDGVVRIPAPALVVNNAATGS"
     gene            2449..>2710
                     /locus_tag="AC158Q_01045"
     CDS             2449..>2710
                     /locus_tag="AC158Q_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010101417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-carbohydrate
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR3338383.1"
                     /translation="MLQREVEIINKLGLHARASAKLTQAAGRFKASVWVGRNGRRVNA
                     KSIMGVMMLAAAMGSKIVVETDGPDEEQAMDAVTRLIADRFEE"
BASE COUNT          502 a          929 c          861 g          418 t
ORIGIN      
        1 tcgatactgc ggcaggccgg catccaggtc cgcatcggca cgctgctcgc ggacatcacc
       61 gggccgaccg aggtagagat cgaaggcggc gggcgcctca agctcgagcc gctggagcgc
      121 cgcggcaacc ggctggcggt gaacgggttc gatccctgcg tggtgctcct caacaacgat
      181 ctctcctcgg gcacgccgga aatcctgatg aacctcgagc aggcggtgct gccgccgctg
      241 cacgccggct ggaccgtgcg ccgcaaatcg aaccatttcg cggcctaccg ccaggtggcc
      301 gaggagttcg ccgggctgct cggcatcgat ccctggttca tcaaccccta tttcgagaag
      361 tgcggcaaga tcaacttcca ggaaaaaaag ggcgaggaat gcctcgaggg ctacgtcgcc
      421 gagatcctcg atgacatccg cgccaagtac aaggaatacg gcatcaccca ggaccccttc
      481 gtcatcgtca aggccgacgc cggcacctac ggcatgggcg tcatgacggt gaaggacccc
      541 accgaagtgc gggagctcaa ccgcaagcag cgcaacaaga tggcggtggt gaaggaaggt
      601 ctcgaggtgc gcgaagtgat ggtgcaggag ggcatctata ccttcgagag cgtggacgac
      661 gcggcggcgg agccggtggt ctacatgatc gaccatttcg tggtgggcgg cttctaccgc
      721 gtgcacaccg cgcgcggcca ggaccagaac ctcaacgcgc ccggcatgca gttcaagccg
      781 ctcgccttcg ccgagccctg ttcctcgccc aacccggacg acccgggctg cccgcccaat
      841 cgtttttacg cttacggcgt catcgcccgg ctcgcgctgc tcgccgccgc gatcgagctc
      901 gagcagaccg aggccgcgct cgaggccggc gccgcgccgc gcatcgccgc cgcttcatga
      961 agctcgcttt cgtcgtcgat ccgctcgaca gcttcaaaat ccagaaggac accactttcg
     1021 ccatcatgcg cgaggcggcc gggcgcggcc acgcgctctt tgccatggag caggacgacc
     1081 tgtcctggca cggtggcacg gttaccggca actcgagaga actgcacctg accggcgaga
     1141 agccgcgctg gtaccgcgcc ggcgagccgg ccgccacgcc gctccaggcg ttcgacgcgg
     1201 tgctgatgcg caaggacccg ccgttcgaca tggagtacgt ctacagcacc taccttctcg
     1261 agctggcgga attacacggc gccagggtca tcaaccgccc gcgcgcgatc cgcgactgga
     1321 acgagaagat gggcatcacg cgctttccgc aattcgcgcc gcccaccatg gtgacgcggc
     1381 gcgaccggtt gatccacgaa ttcctcgccg agcaccgcga cgtgatcctg aagccgctcg
     1441 acggcatggg cggcgcgtcg gtcttccgcg tgcgccagga tgatcccaac gtcaacgtca
     1501 tcatcgagac cgtcacgcat cacggccggc gcaccatcat ggcacagcgc tacctcccgg
     1561 aaatccgcga cggcgacaag cgcgtgctgt tgatcggcgg caaacccgtg ccccattgcc
     1621 tggcccgcgt cccccggccg ggagagacgc gcggcaatct cgcggccggc gccaccggcg
     1681 tcgcgcgcga gctgactacg cgcgaccggg agatcgccga agcgctcggt cccgtcctcc
     1741 acaaggaggg cctgctcctc gtcgggctcg acgtgatcgg cgactgtctc accgaggtca
     1801 acgtcaccag ccccacctgt ttccaggaaa tcaccgagca atccggcttc aacgtcgccg
     1861 gcatgatgct cgatgcgctg gagcgggaaa tcggccgccc ggccgccagg aaaatgaagc
     1921 agtagttcgc agcactccat cacgcagcag gaaagggtgc ctggccggcc gctgtttcgg
     1981 ctgcctggtg cggcgcgata gcgtaaaata ctgcagttat atttcagggg cttacccccc
     2041 gttttatggt tggcatactg atcatcagcc acggcacgct cggcgaagcc ctgatccatt
     2101 gcgcgagcca cgtgctgaac aagcgccccc cccgtctcaa gcagctcggc atcaccgccc
     2161 aggacgatcc cctccagctg ctgccgcagg cgcgcaagct ggtgaaggat ctggacgagg
     2221 gcgacggcgt gctgatcctc tcggacatgt acggcggctc gccttccaat atcgcggcca
     2281 agctgctcgt ccccggcaag atcgaaggcg tggcgggagt caacctcccg atgttgatcc
     2341 gggtcctgac ttaccgcgac aagtccctcc agaccatcct caccaaggcg gtgagcggcg
     2401 ggtgcgacgg cgtggtgcga attcccgccc cggcgcttgt cgtcaacaat gctgcaacgg
     2461 gaagttgaga tcatcaacaa gctcgggctg catgcccggg catcggccaa gctgacgcag
     2521 gccgcgggcc gcttcaaggc cagcgtctgg gtgggccgca acggccgccg cgtcaacgcc
     2581 aagagcatca tgggggtcat gatgctggcc gcggccatgg gctcgaagat cgtggtcgag
     2641 accgacgggc ccgacgagga gcaggcgatg gacgccgtca cccggctcat cgccgatcgc
     2701 ttcgaggaaa
//
LOCUS       DBNGSG010000046         5972 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2560710, whole genome shotgun sequence.
ACCESSION   DBNGSG010000046 DBNGSG010000000
VERSION     DBNGSG010000046.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5972)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5972)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5972
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2560710"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="AC158Q_01050"
     CDS             <1..529
                     /locus_tag="AC158Q_01050"
                     /inference="COORDINATES: protein motif:HMM:NF020419.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HIR3338384.1"
                     /translation="PSSRLGRQLAEAAGALNAIGARFALIGGLALAPYKVVRATQDID
                     FLVETEKADDIDAALTKLGYQRLHRGTDAANYTRGDERLDLLYASRPVARQLLDGAGE
                     LDTGLGKLRVVSAEGIIGFKLQALINDPRRTQDLEDIRALVRANRAELDMNEVREYFR
                     LFERESLLDEILREA"
     gene            507..686
                     /locus_tag="AC158Q_01055"
     CDS             507..686
                     /locus_tag="AC158Q_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338385.1"
                     /translation="MKSSAKPEVPFAADGGLRPAPVRENDPYRALDELMAAVEALCPV
                     WPARTEPTITRKMLL"
     gene            complement(781..2436)
                     /locus_tag="AC158Q_01060"
     CDS             complement(781..2436)
                     /locus_tag="AC158Q_01060"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR3338386.1"
                     /translation="MKVYDAVANAFVKEGGTTVFGLLGDGQLTWWAAMAKHPGVRLID
                     AREEGAGLAMAEGWARASGKVGVCSVTHGPGLARLSLSLIAAARSHTPVVVHTATTSW
                     NDERETQYLNQDRFVAAAECGYIEVMKPDYAEEAVRQAFYRARRESRPVVLSIPMDVQ
                     GKECESEGEDYVPSTALGGDQQRIRPARDRVQEAVKMISAAKRPVIVLGRGAKDPQAA
                     EVVERLGKRIGALIATSFYAKGTLNDSDWYAGVSGLFSTRAAMTLFQEADCVIGVGAG
                     LNIKTLGGGYTFPDARIVHIDVQPHLLLGTDRGADCYVQGDATETLGEIEALLAKQGV
                     QRDGFRTAATKNALRGSFRDPGEFEIEPGTVDIREVLTVLDDRLPSSVGLVTGSAHNF
                     TFPVWHMQKPRALQVSVTSFTGVGQVIGNAIGAAYASKDPVCAIDCDGSALQNIQELD
                     TAGRLGVKLLYCIVNDEAYGAEYHKLRAKGLNGNLSAVPTPDFGALARVFGCRGRQAR
                     TLDEVASGIDEFMAVDGPMVLDVRISRNVISIPYRRLHFGQDV"
     gene            complement(2959..3150)
                     /locus_tag="AC158Q_01065"
     CDS             complement(2959..3150)
                     /locus_tag="AC158Q_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338387.1"
                     /translation="METGFVNPVYMILVALIGAGVALVIPGLQPFVQGEPASTWHWIE
                     LGAGTALIVACVAWYVRWK"
     gene            complement(3198..3719)
                     /locus_tag="AC158Q_01070"
     CDS             complement(3198..3719)
                     /locus_tag="AC158Q_01070"
                     /inference="COORDINATES: protein motif:HMM:NF015444.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HIR3338388.1"
                     /translation="MAAKRMVAGGARAGEIVVVRLAYGELLLESIEEICRKRRIRDGV
                     ILTGFGSLTDIAVSGAVGASFPPRKFYRRTRPRGVEILAMAGVIANYHVHCHLVLSDR
                     NRAFGGHLERGCRILSLAEIALMRVTGIRLARLVDATTGQKLLQSVRAYPPVSGAGKE
                     SLHMAASRIGKRR"
     gene            4147..5517
                     /locus_tag="AC158Q_01075"
     CDS             4147..5517
                     /locus_tag="AC158Q_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HIR3338389.1"
                     /translation="MSERFDAVVIGAGQAGPALCARLDREGLKTALIERRRLGGTCVN
                     DGCIPTKTLVGSARAIHMARRGSEYGFAAGEIRVDMAAVKRRKDGVVRQSQEGLAAWI
                     GGMKNVTLIEGHARFTAPRTLSVNGRELAADKVFLNVGGRALVPDIPGVKDVPFLTNS
                     SIMEVDRVPGHLIIVGGSYIGLEFAQMYRRFGSKVTVVEKMPKLLPREDDDVAAEIRA
                     ILEREGVAIHTGAGDMAVNKKGEAIEVRLASGKSVEGSHLLLAVGRVPNTRDLGLDQA
                     GVELDARGYIQVDDRCRTSAEGVWAVGDANGRGAFTHTSWNDHEIVAANLFDNDPRSI
                     SDRIPCYALFIDPPLGRIGMNEAEAGASGRQLLAAKMPMARVGRAREAGETQGFMKVL
                     VDAGSKELLGAAILGLNGDEIVHSLLDVMYAKKPYTTIQRAVHIHPTVTELVPTLLGM
                     LKPVAG"
BASE COUNT          989 a         2075 c         1842 g         1066 t
ORIGIN      
        1 gccatcatcc cgcctcggca gacagcttgc agaggccgcc ggcgcattaa acgcgatcgg
       61 cgcccggttc gcccttatcg gcggtcttgc cctggccccg tacaaggtgg tgcgtgccac
      121 ccaggatatc gacttcctgg tcgagacgga gaaggccgac gacatcgatg cggcgctgac
      181 aaagctgggc taccagcgcc tgcaccgggg aacggatgcg gccaattaca cgcgaggtga
      241 tgagcggctg gacctgctct acgccagccg gccggtagca aggcagctcc tcgacggtgc
      301 cggcgaactg gacaccggcc tcggcaagct gcgcgtggtc agcgcggaag gaatcatcgg
      361 cttcaagctc caggcgctaa tcaatgatcc gcgccgcacg caggatcttg aagatatacg
      421 ggccctcgtc cgggcgaatc gcgccgaact ggacatgaac gaggttcgag agtactttcg
      481 gctcttcgaa cgcgagtcgt tgctcgatga aatcctccgc gaagcctgaa gtgccgttcg
      541 ccgccgatgg cggcctgaga cccgcccccg tgcgcgagaa cgacccgtac cgcgcgctcg
      601 acgaactcat ggcggcggtg gaggccctgt gtccggtctg gcccgcgcga accgaaccga
      661 cgattacccg caagatgctg ctttagccgt tgcgccagcc gtcgttcccg tgccgcgggc
      721 caggcaccca tccttgtgcc ttgtgccttg tgccttgtgc ctcgtgcctt gtgccttcgg
      781 ctagacgtcc tgcccgaagt gcagccgccg gtacggaatg ctgatcacgt tccgtgaaat
      841 cctcacgtcg agcaccatcg gcccgtcgac cgccatgaac tcgtcgatgc ccgaggccac
      901 ttcgtcgagc gtgcgcgcct ggcggccgcg gcagccgaac acgcgcgcga gcgccccgaa
      961 gtccggcgtc ggcaccgccg acaggttgcc gttcaggccc ttcgcgcgca gcttgtggta
     1021 ctcggcaccg taggcttcgt cgttcacgat gcagtagagc agcttcaccc ccagccgccc
     1081 ggcggtgtcg agctcctgga tgttctgcag cgcgctgccg tcgcagtcga tcgcgcagac
     1141 cgggtccttc gacgcgtacg cggcgccgat cgcgttgccg atcacctggc ccacgccggt
     1201 gaaggaagtg acgctcacct gcagcgcccg cggcttctgc atgtgccaca ccgggaacgt
     1261 gaaattgtgc gccgatccgg tcaccagccc gacgctggac ggcagcctgt catcgagcac
     1321 cgtgagcacc tcgcgtatgt ccaccgtgcc gggctcgatc tcgaactcgc ccgggtcgcg
     1381 gaacgagccg cgcagcgcgt tcttcgtcgc ggcagtgcgg aacccgtcgc gctggacacc
     1441 ctgtttcgcg agcagggcct cgatctcccc gagcgtctcc gtcgcatcgc cctgcacgta
     1501 gcagtcggcg ccgcggtcgg tgccgagcag gaggtgcggc tggacgtcga tgtgcacgat
     1561 gcgggcgtcg ggaaaggtgt agccgccgcc cagcgtcttg atattgagcc ccgcgcctac
     1621 cccgatcacg caatcggcct cctggaacag cgtcatggcc gcgcgggtgg agaacagccc
     1681 ggagacgccg gcgtaccagt cggagtcgtt caacgtgccc ttggcgtaga aactcgtcgc
     1741 gatcagcgcg ccgatgcgtt tgccgagccg ctccacgacc tccgcggcct gcggatcctt
     1801 agcgccccgg cccagcacga tcaccggccg cttggctgcg gaaatcattt tcaccgcctc
     1861 ctgcacgcga tcgcgcgcgg gacggatccg ctgctgatcg ccgcccagcg ccgtggacgg
     1921 cacgtagtcc tccccttcgc tctcgcattc cttcccctgc acgtccatcg ggatggacaa
     1981 cacgaccggc cgcgactccc ggcgcgcgcg gtagaacgcc tggcgcaccg cctcttccgc
     2041 gtagtcgggc ttcatcacct cgatgtaacc gcactcggcg gcagcgacga agcggtcctg
     2101 gttgaggtac tgggtctcgc gctcgtcgtt ccagctcgtc gtagcggtgt ggaccaccac
     2161 cggcgtgtgc gagcgcgcgg ccgcgatgag cgacagggag agccgcgcga gccccggccc
     2221 gtgcgtcacg ctgcacacgc cgaccttgcc cgaggcgcgc gcccagccct ccgccatcgc
     2281 caggccggcg ccctcctcgc gcgcatcgat cagccgcacc cccgggtgct tcgccatcgc
     2341 cgcccaccac gtgagctggc catcgcccag caggccgaag acggtcgtcc cgccctcctt
     2401 gacgaatgcg ttggcaacgg cgtcgtagac tttcatgcgt tctcctgtgg cgggtgatga
     2461 gtgacgagaa acggtctaaa taatagccac ggaatcacac agaacgacac ggaccataac
     2521 gcgacgcctt ggagtccccg tcatcattca ggaatatctg cgtggttccg tgtgtttccg
     2581 tggctgaaat ggtttttgag atgacttcga gagacggaat tttactctat ggcgtcgcgg
     2641 cgccagccac gttgaagctt cgacacggag gacacggagg aaaacttttc gcggagcccg
     2701 gttctacgtt ttccgattca cggggtttag cagtttgttg aaatttcccg ttcgcgctga
     2761 gcgtagcgcc acaggcgcga agtcgaagcg tcccacctaa ttgattgcag gccattcata
     2821 tttcgactcc gcttcgctac gctcaatacg aacggccttt tacgcttttt tcaacaaact
     2881 gttagggcac tttccccttt ttccttttcc cttttgcctt gtgccttgtg ccttaagagc
     2941 gcagcccctc cccccttctc acttccaccg cacataccac gcgacgcaag ccacgatcag
     3001 cgccgtgccg gcccccagct cgatccagtg ccaggtcgaa gcgggctcgc cctggacgaa
     3061 cggctgcagc cccgggatca ccagcgccac gcccgcgccg atgagcgcga cgaggatcat
     3121 gtaaaccggg ttgacgaagc cggtttccac gtcgatgcca gttggccctt tgtgcctctt
     3181 gcctgcggtc gccatcgtca gcggcgcttg ccgatcctgg aggcggccat gtgaaggctt
     3241 tctttccccg ccccgctgac cggggggtac gcccggacgc tttgcagcag cttctgcccg
     3301 gtcgtcgcgt ccaccagccg cgcgagcctg atcccggtga cacgcatgag cgcgatctcg
     3361 gcgaggctca gtatccggca cccgcgctcg aggtggccgc cgaacgcgcg gttgcgatcc
     3421 gacagcacga ggtggcagtg aacgtggtaa ttcgcgatga cgccggccat ggccaggatc
     3481 tcgacgccgc gcggacgcgt gcgccggtag aactttcgcg gcggaaacga tgccccgacc
     3541 gcgccgctca ccgcgatatc ggtgaggctg ccgaagccgg tcaggatgac cccatcgcgg
     3601 atgcggcgct tccggcagat ctcctcgatc gattccagga gaagctcgcc gtatgcgagc
     3661 cgcacgacga cgatctcgcc tgcgcgcgcc ccgcctgcca ccattcgctt tgccgccatc
     3721 gccgtaggtc ttgttcgagt ccggaattcc gggtagcggt gatcttacac tgctgtcatt
     3781 tccctctgcg tcgcagcaga attcagaata acccgggaac tccaccctga ccccatatac
     3841 cgacgttcca gaaaggggtg actcgtttct cgtttctcgt ttctcgtttc tcgtttctcg
     3901 tttctcgttt ctcgtgagtc gtgagtcgag cctatcgccc tagcccctca tccctcatcc
     3961 ctcatccctt atccctcatc cctcatccct catccctcat cccttgtgcc tttcgacttt
     4021 cgacttccgc cttgggtctt ccggggggcc tgccctgcaa gattcggcgg gtgacaagct
     4081 atcatgagcg ggaagcgtcc gggagggcaa gcgctcttcc aggcaaaatc cggcaaggag
     4141 gtttccatgt cagagcgatt cgacgccgtc gtcatcggag cgggccaggc gggcccggcc
     4201 ttgtgcgcgc gactggaccg cgaggggctg aagaccgcgc tcatcgagcg ccggcgcctg
     4261 ggcggcacct gcgtcaacga cggctgcatc ccgaccaaga cgctggtcgg cagcgcgcgc
     4321 gccatccaca tggcgcgccg cgggtccgaa tacggtttcg ccgcgggcga gatccgcgtc
     4381 gatatggccg cggtcaagcg gcgcaaggac ggcgtggtcc ggcaatcgca ggaaggcctc
     4441 gccgcctgga tcggcggcat gaagaacgtc accctcatcg agggccacgc gcgcttcacc
     4501 gcgccgcgaa ccctgtccgt gaacggcagg gagctggcgg cggacaaggt gttcctcaac
     4561 gtcggcgggc gcgcgctcgt tcccgacatc ccgggcgtga aggacgtgcc ctttctcacc
     4621 aacagctcga tcatggaggt ggaccgggtg cccgggcacc tcatcatcgt cggcggcagc
     4681 tacatcgggc tggaattcgc gcagatgtac cggcgcttcg gctcgaaggt caccgtggtg
     4741 gagaaaatgc ccaagctcct gccgcgcgag gacgacgacg tcgccgccga gatccgcgcc
     4801 atcctcgagc gcgagggcgt cgccattcac accggcgcag gcgacatggc ggttaacaag
     4861 aagggcgagg ctatcgaagt ccgtctcgcc agcggcaaga gcgtcgaagg cagccacctc
     4921 ctgctcgccg tcgggcgcgt gccgaacacg cgggacctgg ggctggacca ggcgggcgtc
     4981 gagctcgatg cgcgcggcta catccaggtg gacgaccggt gccgcaccag cgccgagggc
     5041 gtctgggcgg tgggcgacgc caacggccgc ggcgccttca cgcacacttc ctggaacgac
     5101 cacgaaatcg tcgccgccaa cctcttcgac aacgatccgc gcagcatcag cgaccggatt
     5161 ccctgctacg ccctcttcat cgacccgccg ctcggccgca tcggcatgaa cgaggccgaa
     5221 gccggggcga gcgggcgcca gctcctcgcc gcgaagatgc cgatggcccg cgtcggccgc
     5281 gcccgcgagg ccggcgagac ccagggcttc atgaaagtgc tggtggacgc cggctcgaag
     5341 gaactgctcg gtgccgcgat cctgggcttg aacggcgacg agatcgtgca ttcgctgctg
     5401 gacgtgatgt acgcgaagaa gccctacacc accatccagc gcgcggtcca tatccatccc
     5461 accgtgacgg aactggtgcc gacgctcttg ggaatgctga aaccggttgc ggggtgagcc
     5521 ccactgcggt ttcaggtact gcgtctcagg tactacgtct caggtaccgc gtctcaggta
     5581 ctacgtctca ggtactgcga tgtttaacct gtctaggacg cctcatctgg gacgcttcat
     5641 ctgcgacgcc tcatctgcga cgcttcacct gcgacgcgtt atctgggacg cttcacctgg
     5701 gacgcttcat ctgcgacgcc cctcttccgc cttccgactt ccgactttcg ccttcaggcg
     5761 aagccctcgt ccgcaaccag cgaccgccca gtcggagacg ggcggaggga aagctttacc
     5821 cctctctcct caccgggcct caggcaggat aatccagctc ccgctggtgc cggatgccca
     5881 ggatccagac ctcgccgcgt tcaacggaaa accagtacag ggcgacgtag ccggtgctgc
     5941 cgtacgagat cacgagctcg cgcagcccgc cc
//
LOCUS       DBNGSG010000047         3541 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4089564, whole genome shotgun sequence.
ACCESSION   DBNGSG010000047 DBNGSG010000000
VERSION     DBNGSG010000047.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4089564"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="AC158Q_01080"
     CDS             complement(<1..518)
                     /locus_tag="AC158Q_01080"
                     /inference="COORDINATES: protein motif:HMM:NF016494.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxD N-terminal domain-containing protein"
                     /protein_id="HIR3338390.1"
                     /translation="MTRSKPESYSLSGIRERFGGEIAGDPATEICQVATLESATPACI
                     AFFANERYLPQLKTTRAGAVIVGESARHATRLPRIVCTNPYAYFARVSALLNPDRPVK
                     PGVHASAVVDATATIAADAEIGPCAVVARNAKIGTGCVIGAGCHVGDGTVIGSGSRLY
                     PNVTVYHDCLIG"
     gene            complement(588..1091)
                     /locus_tag="AC158Q_01085"
     CDS             complement(588..1091)
                     /locus_tag="AC158Q_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH/Skp family outer membrane protein"
                     /protein_id="HIR3338391.1"
                     /translation="MNPTYATLAALLLASVVTTAGAAEIKIGFVDVERIHRESAPAER
                     ASKQLEKEFAPRAQELQRRESQIKTLQSQTEKDAVTMSETERRTREQELSRLGVEYQR
                     MQREYREDLNMRRNQEIAALLERANRVIKQIADAEKFDLILQDAVYRNPRLDITDKVL
                     KALSEGK"
     gene            complement(1149..3416)
                     /gene="bamA"
                     /locus_tag="AC158Q_01090"
     CDS             complement(1149..3416)
                     /gene="bamA"
                     /locus_tag="AC158Q_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HIR3338392.1"
                     /translation="MRWFRAVWLLLALHAAAASAIEPFVIKDIRVEGIQRTEAGTVFS
                     YLPVKVGDTMTDERASQAIRALYSTGFFRDVGLEREGDVLVVVVQERPSIAQIDFSGM
                     HEFNKDQLTNGMRQIGLAEGRIFDRGLLERAEQDLKRQYLSRGYYAVSINTTVTPLER
                     NRVSLNFIVDEGQVAKIRQISIIGAKVFRESVLLDQFVLRTPGMLTWFSKHDQYSRQK
                     LSADLESLRSYYLNRGYLEFSIDSTQVSITPDKQDIYITISITEGQQYRVSEIKVAGE
                     PLMPEEVVRKLIRLTPGEVFSRERLTESTKLIADRLGNDGYAFANVNAVPELDKVKQQ
                     AAFTFFIDPGRRVYVRRVNVTGNNRTRDEVIRREMRQLEGGWYSADKVTLSKQRVDRL
                     GYFTEVNLETPAVAGATDQIDVNVAVVEKPTGLVLFGAGFGSGEGLVLSGSIQQNNIF
                     GSGKHIALGLNTSKINTTYSLSYTDPYFTVDGVSQGFDVYYRVLSPIDFNLARYETKT
                     IGAALRLGIPVSEIDTIQYGFGMENVDIVTFPDSPLFYLDYVNTFGPSNSNVYLSASW
                     VRDGRDSLIYPTKGSLHKASGELGLPVADLQYYKTTYQYQRYFPLTRHLTFMVNGEGG
                     YGDGYDDKPLPFFKNFFAGGVNSVRGYKSYTIGPKDSSGQPRGGSVKLVGNAELFFPF
                     PGLENDRSVRMGTFVDMGMVGDKFESSDMRFSTGLSLLWVSPLGPLKISVAKALRAQP
                     GDRKEVFQFTVGGAF"
BASE COUNT          666 a         1081 c         1042 g          752 t
ORIGIN      
        1 tcgccgatca gacaatcgtg gtagacggtg acattggggt acagtctcga tcccgaaccg
       61 attacggtgc cgtccccgac atggcagccc gcgccgatga cgcagccagt gccgatcttt
      121 gcgttacgtg ccactaccgc gcaagggcca atttcggcat ctgccgcgat ggttgccgtt
      181 gcgtcgacta ccgcgctggc gtgcaccccg ggcttcaccg gacgatccgg gttgagcaat
      241 gccgacacgc gagcgaagta ggcgtaaggg ttcgtgcaca cgatacgagg cagccgcgtg
      301 gcatggcgtg ccgattcgcc gacgatcacg gcgcctgcgc gcgtggtctt cagttgcggc
      361 aggtaacgct cattggcaaa gaatgcgata caagcggggg tggcgctctc cagcgtggcg
      421 acctggcaga tctcggttgc gggatcgccc gcgatttccc ccccgaaacg ctccctgatt
      481 ccgctcaggc tgtaggattc tggtttgctg cgtgtcatga ccgcgaggct taaaattccg
      541 tgagtcgcac gagggcgcgg cgccattgcc tcgctgttag cctccgctta tttgccttcg
      601 gagagcgcct tgagcacctt gtccgtaatg tcgaggcgcg ggttgcggta aaccgcgtct
      661 tgcaggatca ggtcgaactt ctcggcgtcc gctatctgct tgatgacgcg atttgcgcgc
      721 tccagtaatg cggctatttc ctggttccgg cgcatattaa ggtcctctcg atactcgcgc
      781 tgcatgcgct ggtattccac gcccaggcgg gaaagctcct gttccctggt ccgacgctcg
      841 gtctcgctca tggtcaccgc gtccttctcg gtctgggact gcagcgtctt gatctgggac
      901 tcgcggcgct gcagctcctg tgcgcgcggg gcaaactctt tctccagttg tttgctcgcg
      961 cgctccgcgg gagccgactc gcgatgaatt cgctccacgt ccacgaagcc gatcttgatc
     1021 tcggccgccc ccgcggtcgt gacgacggaa gccagcagca atgcggctaa tgtcgcgtat
     1081 gttgggttca agacgctctc cttactcaac cgttggcatg cccatgatgc gggcaactcg
     1141 ccggaaggtc aaaatgcccc gccgactgta aactgaaaaa cttccttgcg gtcgccgggt
     1201 tgcgcccgca atgccttggc gacgctgatt ttgagcggcc ccagtggcga tacccataat
     1261 agcgacaatc cggtagaaaa acgcatatcg ctgctttcga acttgtcgcc gaccatgccc
     1321 atgtccacga aagtgcccat tctcaccgag cggtcgttct ccagtcccgg gaacgggaag
     1381 aacagctcgg cgttgccgac cagcttgacg ctgccccccc gcggctgacc gctgctgtcc
     1441 ttggggccta tagtataaga cttgtagccg cgcaccgagt tgacgccgcc ggcaaagaaa
     1501 ttcttgaaaa atggcaaagg cttgtcgtca tagccatcgc catatccgcc ctccccgttc
     1561 accatgaagg taaggtggcg cgtcagcggg aaatagcgct ggtactgata ggtagtcttg
     1621 tagtactgca agtcggcgac cgggagcccc agttccccgc tggccttgtg cagggagccc
     1681 ttggtggggt aaatcaggct gtcgcgcccg tcccggaccc agctcgcgga caggtacacg
     1741 ttgctgttgc tgggcccgaa cgtattaacg taatccagat aaaacagcgg gctgtccgga
     1801 aacgtgacga tatcgacatt ttccatccca aacccgtact ggatggtatc gatttccgat
     1861 acggggatgc ccagcctcaa cgcggcccct atcgttttgg tctcgtaacg cgcaaggttg
     1921 aagtcgatag ggctcagcac acggtagtac acgtcgaagc cctggctcac tccgtccacg
     1981 gtgaaatacg ggtccgtata agacagggag taggttgtgt tgattttgct cgtgttcaac
     2041 cccagtgcaa tatgcttgcc gctgccgaat atgttgttct gctgtatcga gcccgacagc
     2101 accagcccct ctccgctgcc gaagcccgct ccaaacagca ccaggccggt gggtttttcc
     2161 acgaccgcaa cgttgacatc gatctggtcg gtcgcccccg ccacggccgg cgtctcgaga
     2221 ttgacttcgg tgaagtaccc gagccggtcg acccgctgct ttgacagagt gactttatcc
     2281 gccgagtacc acccgccctc gagctggcgc atctcgcggc gtatcacctc gtcgcgcgtg
     2341 cggttgttgc cggtcacgtt cacccggcgc acatagacac ggcggccggg atcgatgaaa
     2401 aaggtgaaag cagcctgctg cttcaccttg tccagttccg gcacggcatt gacgttggca
     2461 aatgcgtagc cgtcattgcc aagccggtcg gcaataagct tggttgactc ggtgaggcgt
     2521 tcgcgcgaga aaacttcgcc gggcgtgagg cggatcaact tgcgcaccac ttcctcgggc
     2581 atgagtggtt cgcccgccac tttgatttcc gaaaccctgt actgctgtcc ctcggtgatg
     2641 ctgatcgtga tatagatatc ctgcttgtcc ggcgtgatcg acacctgcgt ggaatcaatg
     2701 ctgaattcga ggtagccccg gttgaggtaa tacgatcgca gcgactccag gtccgcggaa
     2761 agcttctggc gcgagtattg gtcatgcttg ctgaaccagg tgagcatgcc cggcgtgcgc
     2821 agcacgaatt ggtccagcaa tacgctttcg cggaaaacct tggcgccgat gatgctgatc
     2881 tggcggattt tcgcgacttg gccttcatcc acgatgaagt tgagcgaaac gcggttgcgc
     2941 tccagcggcg tcacggtcgt attgatgctg accgcataat acccgcggct caggtactgg
     3001 cgcttcaaat cctgttcggc gcgttcgagc aggccgcggt cgaaaatgcg gccttcggca
     3061 aggccgatct ggcgcatgcc gttggtcagt tgatccttgt tgaactcatg catgccggaa
     3121 aaatcgatct gggcgatcga tggccgttcc tgcaccacaa ccacgagcac atcaccttcc
     3181 cgctccaggc cgacgtcgcg gaagaaacct gtcgagtaca gcgcccgtat cgcctgggac
     3241 gccctctcat cggtcatggt gtcgccgacc ttgaccggaa gatagctgaa gaccgtgccc
     3301 gcttcggtac gctgtatgcc ttctacgcgt atatctttga tgacgaatgg ctcgatggct
     3361 gaagccgccg ccgcatgaag cgcgagcagc agccagacgg cgcggaacca gcgcatgaat
     3421 ctcagccgct caccagaagg cgggttatat cgttgtagag ggcgaacgcc atcagagtga
     3481 acagcagcgc cattccgacg tgctgtccga tctcgacgac ccgctccgag atcggacgac
     3541 c
//
LOCUS       DBNGSG010000048         5228 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1712389, whole genome shotgun sequence.
ACCESSION   DBNGSG010000048 DBNGSG010000000
VERSION     DBNGSG010000048.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5228)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1712389"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="AC158Q_01095"
     CDS             <1..408
                     /locus_tag="AC158Q_01095"
                     /inference="COORDINATES: protein motif:HMM:NF025052.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop nucleotide/nucleoside kinase family
                     protein"
                     /protein_id="HIR3338393.1"
                     /translation="GRGIYSTKATAATYRRLGVLAQGILDAGYTVVVDAAFLRRAERE
                     AFRAIAERAGAPFLILDFQAPLDTLRARVAARAVRADDASEADLSVLDRQVALREPLT
                     PAEMAAAVAVDGTRPASPAMWRPVIRRLRRATS"
     gene            complement(651..1910)
                     /locus_tag="AC158Q_01100"
     CDS             complement(651..1910)
                     /locus_tag="AC158Q_01100"
                     /inference="COORDINATES: protein motif:HMM:NF024592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HIR3338394.1"
                     /translation="MLASSEALGISVKVLTSLAIGLLLGLQREQTPSAKAGLRTFALV
                     ALFGTVSALIADATASAWVPAAGLVLVGLMIIAAYHQENGKTEADSGTTTVIALLLCY
                     GLGVMVWHGYSQLAAAIGIVATALLHFKTELHGFSERLSREEVASILQFAVLSFVVLP
                     LLPDEGYGPYGVLNPYHIWLMVVLVSGVSLVGYLALRVLGAGRSLPLVGVFGGMVSST
                     ATTLVYARQGRVHPAMAAGGAVIIAIANLVVLARLAVICLVVAPGTIPTMLPVLAGGF
                     VLGTVPMALRLRRMLHQPDVEMPPLSNPTQLRVALGFGAIYAVVLIGSAWLSGRAGAE
                     GLYAMALVSGFVDIDAITLSSLSLYNGGKAGAAVVATAIGIGYATNVVLKLGVLLVVG
                     GRGMLKDCLPTLLAPVAGIAAGLLLFV"
     gene            complement(1944..2402)
                     /locus_tag="AC158Q_01105"
     CDS             complement(1944..2402)
                     /locus_tag="AC158Q_01105"
                     /inference="COORDINATES: protein motif:HMM:NF013936.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HIR3338395.1"
                     /translation="MKFEIPRPLKSEHEELHAMLVRATREDGALGAAARAVARLMEAH
                     FAKEEQFALPPISLLPRLAAGEVTSDMEEILVLSDRLRNKLGEMLDDHKQIVKALEVL
                     LAEARAQNKVEYVEFAHQLMRHAQTEELVMYPAAILAGRYIRIKLGITES"
     gene            2689..3849
                     /locus_tag="AC158Q_01110"
     CDS             2689..3849
                     /locus_tag="AC158Q_01110"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HIR3338396.1"
                     /translation="MSDSALPCWNLRGRSLQPIVQGGMGIGISAHRLAGNVASLGALG
                     TISSVELRHHHSDLLEQAQGCRDQDALDRLNLTALDREIRMALAIAGGRGAIAVNVMK
                     AVSAHAQYVRQSCESGAHAIVMGAGLPLDLPDMTRDFPEVALIPILSDARGIGIVLKK
                     WLRKNRLPDAIVIEHPRYAGGHLGATRTGEINDPRFDFEPVLEGAFKLMRELGIERAR
                     IPLIPAGGINSHGQLRALLELGAAAAQVGTPFAVTEEGDAHPAFKKVLAEAQPEDIVT
                     IMSVAGLPARAVLTPWLRNYLRHEKGLQANAKADPRRCVPGIKCLAVCGLRDGIASMG
                     QFCIDTRLAYALKGDVKKGLFFRGSEKVPFGAAIRPVAELMRYFLTGAMPAH"
     gene            3964..5199
                     /locus_tag="AC158Q_01115"
     CDS             3964..5199
                     /locus_tag="AC158Q_01115"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015112 - nitrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3338397.1"
                     /translation="MHSTAPGARAPTADAMGRDGRITVGLVVLCQVAHFLTFAAIPLL
                     LPLIRDDLGIGFTEAGMLSAAGMLSYAAAQIPAGYLADRYGPQRLFFIGLLMWSTLCV
                     AFGFTHAFWPALTVLFVAGAFRALHFAPGLALLAAWFPPQRRATAMSLFLLGGAAGSV
                     LLSLVGPYLVARQGWRPTFIAFALLGLAAALIFGALAKDRSRPVKTSLPAASELLGMA
                     RFPILWVCSWLQFVRFAAVMGFNFWLPSFLITDRGFSLPAAGLIMAMSAALSAPSNTL
                     GAYVSDRLRNPPLVIGGALAVLACAAALLPAVGSVPALIAVIAVYSVFLGFYFGPLFL
                     VPVEVLGSRVAGTTIGFSNLFANLGGFACIYALGAIRDLDGSFAWGFTGISGICLAGV
                     ALSVALARMRTRALARAVA"
BASE COUNT          801 a         1816 c         1683 g          928 t
ORIGIN      
        1 gggcgcggca tctactccac gaaagcgacc gccgccacgt atcgccgcct gggcgtgctg
       61 gcgcagggca tcctcgacgc cggctatacc gtggtcgtcg atgccgcgtt tctcaggcgc
      121 gcggaacggg aggctttccg ggcaatcgcg gaacgagccg gcgcgccgtt tctgatcctc
      181 gatttccagg cgccgctcga cacactgcgc gcccgggtcg cggcgcgcgc ggtccgtgcg
      241 gacgatgctt ccgaagccga tctctccgtt ctcgatcgcc aggtcgcctt gcgcgagccg
      301 ctgacgccgg cggagatggc ggcggcggtt gcagtggacg gcacccgccc cgcttcgccg
      361 gcgatgtggc gcccggtgat tcggcgcctg cgccgcgcaa ccagttagag ctactgaacc
      421 gccaaggccg ccaagagaaa ctatatataa gtggatatgc ccacgcgtat gcgtatatct
      481 gcatcttatg gagttatatg tgccggtaca tggattcaga acggctccgg tgctgtctac
      541 tacaagttaa tctgaattct gtatttcttg gcggccttgg cgtcttggcg gttaattgtt
      601 gttcttgaat cgcttggcgg tcttggcggt tcgatttttg tattggtttt tcagacaaac
      661 aacagcaagc ccgccgcgat gccggccacc ggcgcgagca aagtgggcag gcagtccttg
      721 agcatcccgc gcccgccgac caccagcagc acaccgagct tgagcacgac gttcgtggca
      781 tagccgatcc cgatggctgt ggccacgacg gccgcgcccg ccttcccgcc gttgtagagg
      841 ctcaagctgg acagtgtgat cgcgtcgata tccacgaagc ccgacaccag tgccatggcg
      901 tagagcccct cggctccggc gcgcccggac agccaggccg agccgatcag cacgaccgcg
      961 tagatcgccc cgaagcccag ggccacgcgc aattgcgtcg ggttgctgag cggcggcatc
     1021 tccacgtccg gctggtgcag catcctgcgc agccgcagcg ccatcggcac ggtaccgagc
     1081 acgaaccccc ccgcgagtac gggcagcatc gtcgggatag tgccgggcgc gacgaccaag
     1141 cagatcaccg ccaggcgcgc cagcaccacc aggttggcaa tcgcgatgat gacggcgccg
     1201 cccgccgcca tcgcgggatg cacgcggccc tggcgcgcgt acaccagtgt cgtcgccgta
     1261 ctcgacacca tgccgccaaa aacaccgacg agcgggaggc tcctgcctgc gccgagcacg
     1321 cgcagcgcga gatagccgac gaggctcacg ccggacacca gcaccaccat cagccatatg
     1381 tggtacgggt tgagcacgcc gtaagggccg tacccctcgt cgggcagcaa cggcaggacg
     1441 acgaacgaca gcaccgcgaa ctggaggatg ctcgccacct cctcgcgcga cagccgttcg
     1501 ctgaagccgt gaagctcggt cttgaagtgc agcagcgcag tcgcgacgat gccgatcgcc
     1561 gccgcgagct ggctgtagcc gtgccatacc atgacgccga gcccgtagca cagcaagagg
     1621 gcgatcaccg tcgtcgtacc cgagtccgcc tcggtcttcc cgttctcctg gtggtaggcg
     1681 gcgatgatca tcagccccac caggactagg ccggcggcgg gaacccaggc gctggcggtc
     1741 gcgtcggcga tcagcgcgga aacggtgccg aacagcgcga ccagcgcgaa cgtccgcagg
     1801 ccggccttgg cgctcggcgt ctgctcgcgc tgcaagccca gcagcaggcc gatcgcgaga
     1861 ctggtgagga ctttgacgga gatgccaagc gcctccgacg atgcaagcac gccatccatt
     1921 gcggcgccgc gaccgcggag cggtcagctc tccgtgatgc ccagcttgat acggatgtaa
     1981 cggccggcga ggatcgcggc cgggtacatc accaattctt ctgtctgagc atggcgcatc
     2041 aactgatggg cgaattccac gtattcgacc ttgttctgcg cgcgggcctc cgcaagcagt
     2101 acctcgagtg cttttacgat ctgcttgtga tcgtccagca tctcgccgag cttgttccgg
     2161 agccggtcgc tcagcaccag gatctcctcc atgtcggagg tgacctcgcc cgccgccagc
     2221 cgcggcagca acgaaatcgg cggcagcgcg aactgttctt ccttcgcgaa gtgcgcctcc
     2281 atcagcctgg cgaccgcgcg cgcggcggcg cccagcgccc cgtcttccct ggtggcgcgc
     2341 accagcatcg catgcaactc ctcgtgctcg cttttcagcg gtctcgggat ttcgaatttc
     2401 accggcgctc tcctgccagg cgtaacgttt ggggccgggc ggaccgcgcg cctggatagc
     2461 agcccgccaa ctcaccttgg ggaattatcc cgtcccccgg cttacgcaac ctgatccaga
     2521 tcaagcctca aatgccgccc gggcaccttc cctggccagc gcgaccgccg ccgccagcag
     2581 atcaggcgct attggttgat ttatatcaaa aaccctcgcg ggcccgttcg gcatactttg
     2641 ttcattgcac actgcccgca agccgcgatt gcggcgcgtc catccatcat gtccgattcc
     2701 gctctgcctt gctggaacct gcgcggccgt tcgctgcaac cgatcgtgca gggcggcatg
     2761 ggcatcggca tctcggcgca ccggctcgcc ggcaatgtcg ccagcctggg cgcgctcggc
     2821 acgatctcca gcgtggaact gcggcatcac cattccgacc tcctcgagca agcccaaggc
     2881 tgccgcgacc aggacgcgct cgaccggctg aacctgaccg cgctcgaccg ggaaattcgc
     2941 atggcgcttg cgatcgcggg aggacgcggc gcaatcgcgg tgaacgtgat gaaggcggtt
     3001 tccgcgcacg cgcagtacgt ccggcaatcc tgcgagagcg gcgcacacgc catcgtgatg
     3061 ggcgcgggcc tcccgctcga cctgccggac atgaccaggg attttccgga ggtcgcgctg
     3121 atcccgatcc tgtcggatgc gcgcggcatc ggcatcgtgc tcaagaaatg gctgcgcaag
     3181 aaccgcctgc cggacgcgat cgtgatcgag catccgcgct atgccggcgg gcacctcggg
     3241 gcgacgcgca ccggggaaat caacgatccg cgcttcgact tcgagccggt gctggagggt
     3301 gctttcaagc tgatgcgcga gctggggatc gagcgtgccc gcatcccgct gattcccgcc
     3361 ggcggcatca acagtcacgg ccagctccgc gcactcctcg aactcggcgc ggcggccgcc
     3421 caggtcggca cccccttcgc ggtcaccgag gaaggcgatg cccaccccgc gttcaagaaa
     3481 gtgttggccg aggcgcagcc cgaggacatc gtgaccatca tgagcgtcgc gggacttccc
     3541 gcccgtgccg tgctgacgcc gtggttgaga aactacctgc ggcacgagaa aggattgcag
     3601 gccaatgcca aggccgatcc ccggcgttgc gtgccgggca tcaagtgcct cgccgtgtgc
     3661 ggcctgcgcg acggcatcgc ctcgatgggc cagttctgca tcgacacgag gcttgcctac
     3721 gcgctgaagg gcgacgtcaa gaaggggctg ttcttccgcg gctccgagaa agtgccgttc
     3781 ggcgccgcga tccggccggt ggcggagctg atgcgctatt tcctgaccgg cgccatgccg
     3841 gcgcattgac gctgctcatg cgggatccgt cgccgctgcc ccatgacgca cgcaaggatg
     3901 ccccctgccg ttagcttgat gtaacgcaaa ccccggcgcg ccacaacacg ttattttcgg
     3961 gtcatgcact ccaccgcgcc gggagcgcgc gcgcctactg ccgacgccat gggccgcgac
     4021 gggcgcatca ccgtcgggct ggtggtgctg tgccaggtcg cgcacttcct caccttcgcc
     4081 gcgatcccgc tgctgctgcc gctgatccgg gacgacctgg gaatcggttt taccgaagcc
     4141 ggcatgctgt cggccgcggg catgctgagc tacgcggctg cgcagatacc ggcaggctac
     4201 ctcgccgacc gctacggccc gcagcgcctg tttttcattg gcctgctcat gtggtcgacg
     4261 ctgtgcgtgg ccttcgggtt cacgcatgcg ttctggcccg cgctgaccgt cctgttcgtc
     4321 gccggcgcct ttcgcgcgct ccatttcgcg ccgggcctcg cgttgctggc ggcgtggttt
     4381 ccccctcagc gccgtgccac cgccatgagc ctgttcctgc tgggcggcgc ggcaggcagc
     4441 gtcctgctgt cgctcgtcgg tccgtatctc gtggcgcgcc agggatggcg cccgacgttc
     4501 atcgctttcg cgctgctcgg cctggccgcg gcgctcatct tcggcgcgct tgcgaaggac
     4561 cggtcacgcc cggtaaagac cagccttccc gcggcgtcgg aattgctggg gatggcccgt
     4621 ttcccgatcc tgtgggtctg cagctggctg cagttcgtgc gcttcgccgc ggtcatgggc
     4681 ttcaacttct ggctgccgtc gttcctgatc accgaccgcg gtttctcgct gccggccgcc
     4741 ggcctgatca tggcgatgag cgcggcgctc tcggcgccct cgaacacgct cggcgcctac
     4801 gtgtccgacc gcctgcgcaa cccgccgctc gtgatcggcg gcgcgctcgc cgttctcgcg
     4861 tgcgcggccg cgctgctgcc ggcggtcggg tccgttccgg cgctgatcgc ggtcatcgcc
     4921 gtctattcgg tgtttctcgg cttttacttc ggcccgcttt tcctcgtgcc ggtggaagtg
     4981 ctgggaagcc gcgtcgcggg caccacgatc gggttctcca acctgttcgc caatctcggc
     5041 ggcttcgcct gcatctacgc gctgggcgcg atcagggacc tggatggatc attcgcgtgg
     5101 ggattcaccg gtataagcgg gatctgcctc gcgggggtgg cgctgtccgt ggcccttgcc
     5161 cggatgcgga cgcgcgcgct ggcccgggcg gttgcatgac aactgacggc ccgggtcacg
     5221 acttgccc
//
LOCUS       DBNGSG010000049         2406 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3239920, whole genome shotgun sequence.
ACCESSION   DBNGSG010000049 DBNGSG010000000
VERSION     DBNGSG010000049.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3239920"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1329)
                     /locus_tag="AC158Q_01120"
     CDS             complement(<1..1329)
                     /locus_tag="AC158Q_01120"
                     /inference="COORDINATES: protein motif:HMM:NF015168.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HIR3338398.1"
                     /translation="MPNRLASQTSPYLQQHADNPVDWHPWDEEALALARAQDRPILLS
                     IGYSACHWCHVMAHESFEDPEVAAEMNRHYINIKVDREERPDLDQIYQTAHAMLTQRS
                     GGWPLTLFLMPDGTPFFGGTYFPKRARHGMLGFLDLLPRIAEAYREKREEIARQNTAL
                     IEALTRTLPAPGGGREPKRTPLDAAVREFAQAFDDAHGGIGHAPKFPHPFELAFCLRR
                     HALDGGEPALAIPRLTLAKMAEGGLYDQLGGGFCRYSVDETWSIPHFEKMLYDNAALT
                     ALYADAWLVTQAPLFRTVAAQTAAWVIRDMQSPEGGYYSSLDADSEHEEGKFYVWKPD
                     EVRSHLTADEYAVVAPHYGLDGAPNFEGHYWHLRVVKPLGAVAAALGVPPAECERRLD
                     AARAKLLAVRVRRVWPGRDEKILTSWNAMMVRGMARAARVFGEPGWLASAR"
     gene            complement(1395..>2406)
                     /locus_tag="AC158Q_01125"
     CDS             complement(1395..>2406)
                     /locus_tag="AC158Q_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PA2778 family cysteine peptidase"
                     /protein_id="HIR3338399.1"
                     /translation="HRHSRVDQDLPVHETGAVSDVVGRLPAIPVIAGVLFSAVLSLAG
                     CATPQLTALPAQLPATVPPRIELSAVPFYPQEDFQCGPAALATVLADAGVAVSPEALV
                     PQVYLPAREGSLQIELLAAARRHGLAAYPLAPRLADLLQEVAGGAPVIVLQNRAFSFA
                     PLWHYAVVIGYDLPREEIVLRSGTTRRLVMTISNFERTWARSGYWSMMALQPQRLPVT
                     AAEDRYVASVVALERVAPGAAQRAYQTALERWPDNLTARIGLGNTAHAQGDFAAAETA
                     YREATRRHPQAADAWNNLAQALLDLRRRDEALLAARRAVDIGGPSLVEYQSTLKAAVD
                     AH"
BASE COUNT          361 a          817 c          858 g          370 t
ORIGIN      
        1 ccgggccgag gcaagccacc cgggctcgcc gaacacgcgc gcggcgcgcg ccatgccgcg
       61 caccatcatc gcgttccagc tcgtcagtat cttctcgtcg cggcccggcc atacgcgcct
      121 cacgcgcacg gcgaggagct tcgcccgcgc cgcgtcgagc cgccgctcgc actccgccgg
      181 cggcacgccc agtgcggctg ccacggcgcc gagcggcttc accacccgca ggtgccaata
      241 atggccttcg aaattgggcg cgccgtcgag accgtagtgc ggcgcgacca cggcgtattc
      301 gtccgcggtg aggtggctcc tcacctcgtc gggcttccag acgtagaact tgccctcctc
      361 gtgctcggag tcggcgtcga gcgaggagta gtagccgccc tccggggatt gcatgtcgcg
      421 gatgacccag gcggcggttt gcgccgccac cgtcctgaac agcggcgctt gcgtcacgag
      481 ccaggcgtcg gcgtagagcg cggtgagcgc cgcgttgtcg tagagcatct tctcgaaatg
      541 cgggatgctc caggtctcgt ccacgctgta gcggcagaaa ccgccgccca gctggtcgta
      601 gaggccgccc tcggccatct tcgcgagagt cagccgcgga atcgcgaggg ccggttcgcc
      661 gccgtccagc gcgtgacggc gcaggcagaa cgcgagctcg aacgggtgcg gaaatttcgg
      721 cgcgtgcccg atgcccccgt gcgcgtcgtc gaacgcctgc gcgaactcgc gcaccgccgc
      781 atcgagcggg gtgcgtttcg gctcgcgtcc gccgcccggc gccggcagcg tgcgcgtgag
      841 cgcctcgatg agcgccgtgt tctgccgcgc gatctcctcg cgcttctcgc ggtaggcctc
      901 cgcgatgcgc ggcaggaggt cgaggaaacc cagcatgccg tggcgcgcgc gcttggggaa
      961 ataggtgccg ccgaagaacg gcgtgccatc gggcatcagg aacaaagtga gcggccagcc
     1021 gccgcttctc tgtgtcagca tggcgtgcgc ggtctgatag atctggtcga ggtcggggcg
     1081 ttcctcgcgg tcgaccttga tgttgatgta atgccggttc atctccgccg ccacttccgg
     1141 gtcctcgaag gactcgtggg ccatgacgtg gcaccagtgg caggccgagt agccgatcga
     1201 gagcaggatg ggccggtcct gggcgcgagc gagcgcgagt gcctcctcgt cccagggatg
     1261 ccagtccacg ggattgtccg cgtgctgctg caggtaaggg gaagtctggg aggcgaggcg
     1321 gttgggcatg atggcggcgc gcaaccggat tctgtcacga tgcgcacacc ggcgcacccc
     1381 cgcgccggcg ctgctcaatg cgcgtcgaca gccgctttca gcgtcgactg gtattcgacg
     1441 aggctcgggc cgccgatgtc gaccgcgcgc cgcgcggcga gcagcgcctc gtcccggcgg
     1501 cgcaggtcga gcagagcctg ggcgagattg ttccaggcgt cggctgcttg cggatggcgc
     1561 cgggtcgctt cgcggtaagc cgtttcggca gccgcgaaat cgccctgcgc atgggccgtg
     1621 ttgcccagac cgatgcgcgc ggtcaggtta tccggccagc gctcgagcgc tgtttgatag
     1681 gcgcgctgcg ccgcgccggg cgcaacccgt tccagggcaa cgaccgaggc gacgtaacgg
     1741 tcttcggctg cggtcaccgg cagccgttgc ggctgcagcg ccatcatgga ccagtacccg
     1801 ctgcgtgccc aggtgcgctc gaagttgctg atcgtcatga ccagccgccg cgtcgtgccg
     1861 gagcgcagca cgatctcttc gcgcggcagg tcgtaaccga tgaccacggc gtaatgccag
     1921 agcggcgcga agctgaaggc gcggttttgc agcacgatga ccggcgcgcc gccggccact
     1981 tcctggagga ggtccgccag ccgcggggcg agagggtaag ccgccagtcc gtggcggcgt
     2041 gccgccgcca gcaactctat ctgcagcgat ccttcccgcg cgggcagata aacctgcggg
     2101 accagcgcct cgggcgatac cgccacccct gcgtcggcca gcacggttgc cagcgcggcc
     2161 gggccgcact ggaaatcttc ctgcgggtag aacggaaccg cggacagctc gatgcgtggc
     2221 ggcaccgttg ccggcagctg cgcgggcagc gcggtaagct gtggtgtcgc gcagccggcg
     2281 agcgacagga cggcggaaaa caaaaccccc gcgatgaccg ggattgccgg caggcggccc
     2341 acgacgtcgc ttaccgcacc ggtctcgtga acgggaagat cttggtcaac ccgagaatgt
     2401 cggtga
//
LOCUS       DBNGSG010000050         1711 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_693864, whole genome shotgun sequence.
ACCESSION   DBNGSG010000050 DBNGSG010000000
VERSION     DBNGSG010000050.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_693864"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="AC158Q_01130"
     CDS             complement(<1..283)
                     /locus_tag="AC158Q_01130"
                     /inference="COORDINATES: protein motif:HMM:NF021856.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2437 domain-containing protein"
                     /protein_id="HIR3338400.1"
                     /translation="MKFGRFDQQGRIFYGAVESDQVTELEGSIFERYKATSKKYPLSA
                     LKTLIPCVPPTFYCAGLNYAAHVEWANQRKGTNTKLPEKADVGYRANNAL"
     gene            complement(331..687)
                     /locus_tag="AC158Q_01135"
     CDS             complement(331..687)
                     /locus_tag="AC158Q_01135"
                     /inference="COORDINATES: protein motif:HMM:NF020376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HIR3338401.1"
                     /translation="MILVIVAGTPPSWGAEAVIVMQRSPLAGFRHYDGREVWREMKPG
                     DRLQLVREPDNPYDANAVRVEWRGVKLGYVPRGDNASVSRQMGRGVMLEARVAALREN
                     RNHTVRIEFEIVAPLQ"
     gene            complement(751..1164)
                     /gene="hemJ"
                     /locus_tag="AC158Q_01140"
     CDS             complement(751..1164)
                     /gene="hemJ"
                     /locus_tag="AC158Q_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HIR3338402.1"
                     /translation="MLWIKSFHLIFLVAWFAGLFYLPRLFVYHAMAEDAASRERFKVM
                     ERKLYYGIMAPGAVLTIAFGLWLWLGYGVSGGWLHAKLALVAVLVAYHLWCGKLLGDF
                     RHDRNTRSHVWYRWFNEFPTVILIAVVILTVVKPF"
     gene            complement(1229..>1711)
                     /locus_tag="AC158Q_01145"
     CDS             complement(1229..>1711)
                     /locus_tag="AC158Q_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018418194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3338403.1"
                     /translation="IMNAPVTLDDVVLGEAVWAASKSFLSGMAVLLVAWALGVVQSAL
                     ALWAIPILFLTGLAFSCLGLIMTALSPSYDFFMYYFTLVITPMMLVCGVFFPVSQLPG
                     PFQAVTQFLPLTHAVELVRPLMSGAVPSHIALHVAVLAAYAAAGFYIALVLTRRRLLT
                     "
BASE COUNT          350 a          535 c          550 g          276 t
ORIGIN      
        1 ccagggcatt gttcgcgcgg tagccgacgt cggctttttc cggaagcttg gtgttggtgc
       61 ccttgcgctg gttggcccac tcgacgtgcg cggcgtagtt caacccggcg cagtagaagg
      121 tgggcggcac gcagggaatc agtgtcttca gggcggacag cggatatttc ttcgacgtag
      181 ccttgtaacg ctcgaagatg gagccttcga gctcggtcac ctggtcactt tcgacggcgc
      241 cgtagaaaat acggccctgc tgatcgaaac gtccaaattt catcgatact cccggaacgg
      301 cgtgaatagt gaacggtgaa ttggaaccgg ttactgcagc ggtgccacta tctcgaactc
      361 gatgcgcacg gtgtggtttc ggttctcgcg cagggccgcc acgcgtgctt cgagcatgac
      421 accgcggccc atctgccgcg acaccgaggc gttgtccccg cgcggcacgt agcccagctt
      481 cacgccgcgc cactcgacgc gaaccgcgtt ggcatcatac ggattatccg gttcccgcac
      541 cagttgcagg cggtcgcccg gcttcatttc ccgccacacc tcccggccgt cgtaatgccg
      601 gaagccggca agcggcgaac gctgcatcac gatgaccgcc tccgcgcccc atgacggcgg
      661 agtgccggcc acgatgacca gaatcatcgc ggctcgcatt ttcgtgccca ggctcatata
      721 aggtttaccg gcgtcgggcc ggtaaacctc ctaaaacggc ttgacgacgg tcaggatgac
      781 aaccgcgatc aggatgacgg tcggaaactc gttgaaccag cggtaccaga cgtggctgcg
      841 ggtgttgcgg tcgtgcctga aatcacccag gagcttgccg caccacaggt gataggcgac
      901 gaggacggcg accagcgcga gcttggcgtg cagccagccg ccgctcacgc catagcccag
      961 ccacagccac aggccgaaag cgatggtcag caccgcgccc ggcgccatga tgccgtaata
     1021 gagcttgcgc tccatcacct tgaagcgttc gcggctcgcc gcgtcctccg ccatggcgtg
     1081 gtatacgaac agccgcggca ggtagaacag gcccgcgaac caggccacca ggaagatgag
     1141 atggaaggat tttatccaga gcatgtctga tttgagtggc gagtgtcgag tggcgagggt
     1201 cgagtggcga gtggcgagtg gcaccatcct acgtcaggag gcggcggcgc gtcagcacca
     1261 gcgcgatata gaaaccggcc gctgcatagg cggccagcac ggcgacgtgc agggcgatgt
     1321 gggagggcac ggcgccgctc atcaacggac gcaccagctc caccgcgtgg gtgaggggca
     1381 ggaattgggt aaccgcctgg aacgggcctg gcagctgcga caccgggaag aagacgccgc
     1441 acaccagcat catgggggtg atcaccagag tgaagtagta catgaagaag tcgtaactgg
     1501 gagaaagcgc ggtcatgatg agccccaggc acgagaaggc gagccccgtc aggaacagga
     1561 tcgggatcgc ccacagcgcc agcgccgact gcacgacccc cagcgcccac gccaccagca
     1621 gcacggccat gccggaaaga aaactcttgc tggctgccca taccgcctcg cccagcacca
     1681 cgtcgtcgag cgtcaccggg gcgttcatga t
//
LOCUS       DBNGSG010000051         6070 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1883000, whole genome shotgun sequence.
ACCESSION   DBNGSG010000051 DBNGSG010000000
VERSION     DBNGSG010000051.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6070)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6070)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6070
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1883000"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1400)
                     /locus_tag="AC158Q_01150"
     CDS             complement(<1..1400)
                     /locus_tag="AC158Q_01150"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3338404.1"
                     /translation="MKTPLEVLKLYPEHDYTLRGAFASRARNDPRRPFMQFAGRTWSW
                     AEFGAAAERAACLFAARGVRQGDRVAVMGRNHEGHVLALFALARLGAVMVPVNPEFGV
                     KEARYVLHHAGVSGVITAGETLDTARRACEGLAAAPWFLLLDAARDGAPNFLEETGRA
                     PRCALPDGATGDATCLIVYTSGTTGFPKGAMHSQRSFVTCGEAFIQRMYLQPDERIMV
                     LLPLFHVNAQFYSVAGTLAAGCSMVVVPRFSASTFWDTAVETGATEVNIIEAIGTILR
                     NRPRSEFRPAHRIRRLYGARQPVMDTFRDEFGIPHLIGGFGMTEIPGVTSTPFEGAQK
                     PGSMGPVGRHPDPARPWAQCRVVDDEGRDVPANEAGELVVKTPIIMQGYFRDPEQTRA
                     AFRDGWFLTGDLVRRDEEGWFYFVARKKDIIRRRGENISGPELDRVIGEHPAVHEAAA
                     IAVASELGEDEILVAVT"
     gene            complement(1533..1952)
                     /locus_tag="AC158Q_01155"
     CDS             complement(1533..1952)
                     /locus_tag="AC158Q_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3338405.1"
                     /translation="MRRALMKDPLFRKGLKIRKAVVGKEFVERRLAAADAIAWPFEEL
                     TTKFAWGAVWTRPALSRKTRSFLNLAALTAMNMRHELKTHIRAALRNGLTRKEVGEAL
                     LHCAVYCGFPKSLDALRLMQEVFSEMDAAAGRGKKRR"
     gene            complement(1991..2869)
                     /locus_tag="AC158Q_01160"
     CDS             complement(1991..2869)
                     /locus_tag="AC158Q_01160"
                     /inference="COORDINATES: protein motif:HMM:NF025091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HIR3338406.1"
                     /translation="MNHTEQRKRLRAVFAGTKCLSPASVYDPLSARVAESVGYELGML
                     AGSVASNTTLAAPDLIVLTLTEFADQVRRIMRASNLSLIVDADHGYGNALNVMRTVEE
                     LEHAGVSGMSIEDTVLPTRFGQTEGADELITLDEMVGKLKAAVGARRDPSTVILGRTA
                     ALKVEGTEGTVKRAKAFAACGVDAIFVVGVESVDQVRAVHEAARLPIMVGSAPASIKR
                     EDFAAAGGRVLLQGHQPIPAAVKALKEVYAHLFSGGAPAELKDKLAPAGDMDKLVNAA
                     AYKTWQRDYLGVKSKG"
     gene            complement(3046..4485)
                     /locus_tag="AC158Q_01165"
     CDS             complement(3046..4485)
                     /locus_tag="AC158Q_01165"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019543 - propionate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate
                     dehydratase/aconitate hydratase"
                     /protein_id="HIR3338407.1"
                     /translation="MSQPTNTRPDPDKVLVQLADYVDRYQVRSELALETARLALIDTL
                     GCGLEALSYPGCTSLLGPVVPGTVVPNGARVPGTPYELDPERATFNIGALNRWLDWND
                     AFYGATVIHPSDTFCGLLAVADWLSRTRVAQRKKPLLVRDVLESAVKTYEIMGGLALE
                     NGFTAAGLDHTILVKIATAPVVTKLLGGTHEEIVNALSQAWVDGHALATFRRKPNTGP
                     RKSWAAGDAASRGVRLALMALKGEIGCPSALTAKTWGFYHVLFRDKPFRFQRPLGAYV
                     MENTLFKIPYPTAFHGQTGVEAAIKLHPLVKDRLDDIKRVEARCHNSTMVILDKSGPL
                     HNFADRDHCMQYMMSIGLIFGTMTAEHYYDHIAADPRIDRLRAKFHLAESKQYEREYH
                     DPKKRTNANSIQVFFKDGTKTPLSEVLYPLGHRKRRQEGTPVLLSKFETAVGRVFAAK
                     RRDAIVSACLDPKRLAAMPVNEFMDLLAV"
     gene            4564..5823
                     /locus_tag="AC158Q_01170"
     CDS             4564..5823
                     /locus_tag="AC158Q_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-dependent oxidoreductase"
                     /protein_id="HIR3338408.1"
                     /translation="MKTRNDILIIGAGIGGLTLALTLHRAGMPCRVYEAAPEIKGLGL
                     GINVLPHATKELAALGLEPALAGIAVTTREALFYNRYGQLVYSEPCGRHAGYDTPQFS
                     IHRGDLQAVLLEACRARIGADRIVTGWRCARAEQDDSGVTVHFTAAESGEALPPQLGA
                     AAVSCDGIHSVLRKQFYPDEGDPIYSGVNMWRGATWWKPFLTGESFVRAGWLESGKMI
                     IYPIRNRLDAHGRQLISWVGEFYTPKYKRRDWNRAGELKDFIHVFEDWKFDWLDVPGM
                     MRNAETILEYPMVDQEPLPRWSHGRITLLGDAAHPMVPRGSNGAGQAILDAVALAECL
                     NECLKRHMDPVEALKQYEARRLEATASIVRMNRKNPPDAILREVFLRTGDKPFQNIDD
                     VISREELAALTHGYARVAGYDKESLKA"
     gene            5835..>6070
                     /locus_tag="AC158Q_01175"
     CDS             5835..>6070
                     /locus_tag="AC158Q_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HIR3338409.1"
                     /translation="MSTTPAKNPKRREFYDRISRHDMAPLWEVLGALVPPTPATPCLP
                     ALWKYRDVRSYIMEAGELISAAEAERRVLVLENPG"
BASE COUNT         1027 a         2038 c         1966 g         1039 t
ORIGIN      
        1 gtcacggcga cgaggatctc gtcctcgccc agctcgctcg ccaccgcgat ggccgccgct
       61 tcgtgcaccg cgggatgctc gccgatcacg cggtcgagct cgggccccga gatgttctcg
      121 ccgcggcgcc ggatgatgtc tttcttgcgc gcgacgaaat agaaccagcc ctcctcgtcg
      181 cggcgcacca ggtccccggt caggaaccag ccgtcgcgaa acgccgcccg ggtctgctcc
      241 gggtcgcgga aatagccctg catgatgatc ggggttttca ctacgagctc acccgcctcg
      301 ttcgccggca cgtcgcgccc ctcgtcatct accacgcggc actgtgccca gggccgcgcg
      361 ggatccgggt ggcgtccgac cgggcccatg ctgcccggct tctgcgcccc ttcgaagggc
      421 gtggaggtaa cgcccggaat ttcggtcatg ccgaagccgc cgatcaggtg cgggatgccg
      481 aactcgtcgc gaaaggtgtc catgaccggc tggcgggcgc cgtacagccg gcgtatgcgg
      541 tgcgcgggac ggaactcgct gcgcggacgg ttcctgagga tggtgccgat cgcctcgatg
      601 atgttgacct cggtcgcgcc ggtctcgacg gcggtgtccc agaaggtgga cgcggagaac
      661 cgtggcacga cgaccatcga gcaaccggcc gcgagagtgc ctgcgaccga atagaactgc
      721 gcgttcacgt gaaacagcgg cagcagcacc atgatgcgct cgtcaggctg caggtacatg
      781 cgctgtatga aggcctcgcc gcaggtcacg aagctgcgct ggctgtgcat cgcgcccttg
      841 ggaaagccgg tcgtgcccga ggtgtagacg atcaggcagg tggcatctcc ggtagcgccg
      901 tccggcaggg cgcatcgcgg cgcgcgcccg gtctcttcca ggaaattcgg cgcgccgtcg
      961 cgagccgcat cgagcagcag aaaccacggc gccgccgcca gcccctcgca cgcccggcgc
     1021 gccgtatcga gcgtctcgcc cgccgtgatc acgccgctca ccccggcgtg gtgcagcacg
     1081 tagcgcgctt ctttcacgcc gaactcggga ttgaccggca ccatgacggc gcccaggcgc
     1141 gccagcgcga acagcgcgag gacatgcccc tcgtggtttc gccccatcac cgccacgcga
     1201 tcgccctgcc tcacgccgcg cgcggcgaac aggcaagccg ccctctccgc cgccgcgccg
     1261 aattccgccc agctccaggt tcttccggcg aactgcatga acggccgccg cggatcattc
     1321 cgtgcgcggc ttgcaaacgc gcccctcagc gtgtagtcgt gctccgggta gagcttcagg
     1381 acttcgagag gtgttttcaa gcgtgactct tgtctggctg gttcagtgca gcttcgttgt
     1441 cgcgtgttga actgcgagta atcgtcagtt ccaacacgcg gagttaaacc ggcgctctgc
     1501 gtccggattt aacggcactt ggcggctaat ttctaacgac gtttcttgcc gcggccggcc
     1561 gcggcgtcca tctcggagaa gacttcctgc atcagccgca gcgcatccag cgacttggga
     1621 aaaccgcagt agacggcgca gtgcagcagc gcttccccga cttccttgcg cgtgaggccg
     1681 tttctcagcg ccgcgcgaat gtgggtcttc aactcgtgcc gcatgttcat cgcggtgagc
     1741 gcggcgaggt tgaggaagct gcgcgtcttg cgcgacagcg cggggcgcgt ccagaccgcg
     1801 ccccaggcaa atttcgtcgt gagctcctcg aacggccagg caatcgcgtc ggcggcggca
     1861 agccggcgct cgacgaactc cttgccgacc accgccttgc ggattttcag gcctttcctg
     1921 aacaggggat ccttcatcaa cgctcgacgc acggtattct ccttactcct catccctcat
     1981 ccctccgccc tcatcccttg ctttttacgc cgagatagtc gcgctgccag gtcttgtacg
     2041 ccgcggcgtt gacgagcttg tccatgtccc ccgccggcgc gagcttgtcc ttgagttccg
     2101 ccggcgcgcc gccgctgaat agatgcgcgt agacctcctt cagcgccttc acggccgccg
     2161 ggatcggctg gtgcccctgc aacaggaccc gcccgccggc tgccgcgaag tcctcgcgct
     2221 tgatcgaggc cggcgccgac ccgaccatga tcggaagcct ggccgcttcg tgcacggccc
     2281 tcacctggtc caccgactcc accccaacca cgaagatcgc gtccacgccg caagcggcaa
     2341 aagctttcgc ccgcttcact gttccctccg tgccctccac cttgagcgcg gcggtgcgcc
     2401 cgagtatgac ggtcgagggg tcgcgccgcg cgccgaccgc ggccttgagc ttcccgacca
     2461 tctcgtcgag cgttatcagc tcgtcggcgc cttcggtctg gccgaagcgg gtcggcagca
     2521 ccgtgtcttc gatgctcatg cccgaaacgc ccgcgtgctc cagctcctcc accgtgcgca
     2581 tgacgttcag cgcgttgccg tagccgtggt cggcatcgac gatgagggaa agattggatg
     2641 cccgcatgat gcgccgcacc tggtcggcga actcggtcag cgtcagcacg atgagatcgg
     2701 gcgccgccag cgtcgtgttc gacgcgaccg acccggccag catgccgagc tcgtagccga
     2761 ccgattcggc gacgcgcgcc gacaggggat cgtaaacgct cgcgggggac aggcacttcg
     2821 tcccggcgaa taccgcgcgc agcctcttgc gctgttccgt gtgattcatg ctttctccca
     2881 ggaaacgaat gaatggacag aattaacaga atgattcaaa aacccgccaa aaccatcaat
     2941 agacaggatt tacaagattt acaggattag atcaaaaaaa caaaacagaa aagccggtat
     3001 ttgttttctg aaccttgtta atcctgttaa tcctgtctat tgcacttaaa cagcaagcaa
     3061 atccatgaac tcgttcaccg gcatcgccgc gagccgcttc gggtcgaggc aggccgagac
     3121 gatcgcgtcg cgccgcttcg cggcgaacac gcggccgacc gcagtctcga acttgctcaa
     3181 cagcaccggc gtgccctcct gccggcgctt gcggtggccg agcggataga gcacctccga
     3241 cagcggcgtc ttcgtgccgt ccttgaagaa gacctggatc gaattcgcgt tggtgcgctt
     3301 cttcgggtcg tgatactcgc gctcgtactg cttcgactcg gcgaggtgga acttggcgcg
     3361 cagcctgtcg atgcgcgggt cggccgcgat gtggtcgtag tagtgctcgg ccgtcatcgt
     3421 gccgaagatc aggccgatgg acatcatgta ctgcatgcag tggtcgcggt cggcgaagtt
     3481 gtgcagcggg ccggacttgt cgaggatcac catcgtcgag ttgtggcagc gcgcttccac
     3541 gcgcttgatg tcgtccagcc ggtccttcac cagcgggtgc agcttgatcg ccgcctccac
     3601 gccggtctgg ccgtgaaaag ctgtcggata cggtatcttg aacagggtgt tctccatgac
     3661 gtaggcgccg agcgggcgct ggaaccggaa cggcttgtcc ctgaacagca cgtggtagaa
     3721 gccccacgtc ttcgccgtca gcgccgaggg gcagccgatc tcccctttca gcgccatcag
     3781 cgcaaggcgc accccgcggc tcgccgcgtc gcccgccgcc cacgacttgc gcgggccggt
     3841 attgggcttg cggcggaagg tcgcgagcgc gtgcccgtcc acccacgcct gcgagagggc
     3901 attgacgatt tcctcatgcg tgccgccgag cagtttcgtc acgaccgggg ccgtcgcgat
     3961 cttcaccagg atggtgtggt cgagcccggc cgcggtgaac ccgttctcga gcgcgagccc
     4021 gcccatgatc tcgtaggtct tgaccgcgga ctcgagcacg tcgcgcacca gcagcggctt
     4081 ctttcgttgc gccacgcgcg tgcgcgacag ccagtccgcc accgcgagga ggccgcagaa
     4141 ggtgtcggac ggatgaatta cggtcgcgcc gtagaacgcg tcgttccagt cgagccagcg
     4201 gttgagagcc ccgatattga acgtcgcgcg ctcggggtcg agctcatagg gcgtgcccgg
     4261 cacgcgggcg ccgttgggaa cgacggttcc cgggacgacg gggccaagca ggctggtgca
     4321 cccggggtag gacagcgcct cgaggccgca gccgagcgtg tcgatcagcg cgagccgcgc
     4381 ggtttcgagg gcaagctcgc tccggacctg gtagcggtcg acgtaatcgg caagctggac
     4441 gaggacttta tcgggatcgg gacgggtgtt ggtgggctgg ctcatgacgg tgatcgatgt
     4501 aagagaagcc gaagtatagg gcaattcacg agcgccaacc cgagggtaaa atgggtccag
     4561 atcgtgaaaa cgcggaacga cattctcatc atcggcgccg gcatcggcgg gctgacgctc
     4621 gcgctgactt tgcaccgggc cgggatgccg tgccgcgtct acgaggccgc gccggagatc
     4681 aagggactcg gcctcggcat caatgtactg ccgcatgcga cgaaggaact cgccgcgctc
     4741 ggactggagc ccgcgctggc gggcattgcg gtgacgacgc gtgaagcgct gttctacaac
     4801 cgctacggcc agctggtcta cagcgagccg tgcggccgcc acgctggcta cgacactccg
     4861 cagttctcga ttcatcgcgg cgacctgcag gcggtgctcc tggaagcgtg cagggcacgc
     4921 atcggcgcgg accgcatcgt caccggctgg cgttgcgcgc gcgccgagca ggacgacagc
     4981 ggcgtgacag tgcatttcac cgccgccgaa tccggcgagg cgctgccgcc gcagctcggg
     5041 gccgccgccg tcagctgcga cggcatccac tcggtgctgc gcaagcagtt ctacccggac
     5101 gaaggcgacc cgatctactc gggcgtcaac atgtggcgcg gcgcgacctg gtggaagcct
     5161 ttcctcaccg gcgagagctt cgtgcgcgcc ggctggctgg aatcggggaa gatgatcatc
     5221 tacccgatcc gcaaccggct cgacgctcac ggccggcaac tcatcagctg ggtgggcgag
     5281 ttctacaccc cgaaatacaa gcggcgggac tggaaccgcg ccggcgagct gaaggacttc
     5341 atccacgtgt tcgaggactg gaaattcgac tggctcgacg tgcccggcat gatgcgcaac
     5401 gccgagacga tcctcgagta ccccatggtg gaccaggagc cgttgccgcg ctggagccac
     5461 ggccgcatca cgctgctggg cgacgcggcg catcccatgg tcccgcgcgg ttccaacggc
     5521 gcgggtcagg cgatcctcga cgcggtggcg ctggccgagt gtctgaatga gtgcctgaag
     5581 cggcacatgg acccggtcga ggcactgaag cagtacgagg cgcgccggct cgaggcgacg
     5641 gccagcatcg tgaggatgaa ccgcaagaat ccgcccgacg ccatcctgcg ggaggttttt
     5701 ctccggaccg gcgacaagcc gttccagaac atcgacgacg tcatcagccg cgaggagctc
     5761 gccgccctca cccatggcta cgcgcgggtg gcgggctacg acaaggaatc cctcaaggca
     5821 taatggcggc tgccatgtcc accacccccg caaagaaccc caagcgccgc gagttctacg
     5881 atcgcatctc ccggcatgac atggcgccgc tgtgggaagt gctcggcgcg ctggtgccgc
     5941 cgacccccgc cacgccctgc ctgcccgcgt tgtggaaata ccgtgatgtg cgctcgtaca
     6001 tcatggaagc gggtgagctg atctcggccg cggaagccga gcggcgcgtg ctggtgctcg
     6061 agaacccagg
//
LOCUS       DBNGSG010000052         4596 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1543526, whole genome shotgun sequence.
ACCESSION   DBNGSG010000052 DBNGSG010000000
VERSION     DBNGSG010000052.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4596)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4596
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1543526"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..1639)
                     /locus_tag="AC158Q_01180"
     CDS             complement(29..1639)
                     /locus_tag="AC158Q_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006053058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3338410.1"
                     /translation="MLRITEVRLPLDHPEDAIRAAILRRLGIRADELEGYTIFRRAVD
                     ARKPAIVFTYTLDVEVSNEPALLARLKDDRRITRTPNTAYQFVAQAPAALESRPVVIG
                     TGPCGLFAGLLLAQMGFRPIILDRGKIVRERTQDTWGLWRKSVLNPESNVQFGEGGAG
                     TFSDGKLYSQVKDPRHLGRKVLTEFVEAGAPPEILYVAKPHIGTFRLVTMVESLRETI
                     QSLGGEIRFQCKVTDVEIEGERVRGVGLENGERIAADHVVLAVGHSARDTFQMLHDRG
                     VHIEAKPFSIGLRIEHPQSLIDRARYGRNAGHPLLGAADYKLVYHCGNGRSVYSFCMC
                     PGGTVVAAASEPGRVVTNGMSQYSRNERNANSGIVVGITPADYPGHPLAGIAFQRHWE
                     ERAYEAGGSNYCAPAQRVGDFLAGRPSTAQGTVVPSYQPGVRWTDLSTCIPGYAVAAI
                     REALPAFERQIRGYAMEDAVLTGVETRTSSPISIRRGTDYQSVNTRGLYPAGEGAGYA
                     GGILSAAIDGIEVAEAVALDIVGETRLR"
     gene            1818..2600
                     /locus_tag="AC158Q_01185"
     CDS             1818..2600
                     /locus_tag="AC158Q_01185"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009769869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HIR3338411.1"
                     /translation="MLKGKTALVTGSTSGIGLGIAEAFAKEGASIVLNGLGRADEIEA
                     LRAKIAKDHKVAVRYDGADMTRPNTIESMMRKAVAEFGAVDILVNNAGIQHVAPIDEF
                     PVEKWNAIIAINLVASFHTIRLALPAMKKRKWGRIINIASAHALVASPFKSAYVAAKH
                     GVAGLTKTVALEVAEQGITVNAICPGYVRTPLVEKQIPDTAKARGITEEQVVKDVLLA
                     AQSTKQFVTVGQVASLARYLASDDAASITGAILSVDGGWTAH"
     gene            3162..>4596
                     /locus_tag="AC158Q_01190"
     CDS             3162..>4596
                     /locus_tag="AC158Q_01190"
                     /inference="COORDINATES: protein motif:HMM:NF014398.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HIR3338412.1"
                     /translation="MDSASKSRSRILAATGWAVLVTGLLLISATVIPADDAKKGAPPT
                     ITDSTDDPAVWGKTFPLHYELYKKSTDMTRTKHGGSEAMPHKPTPGDPRSKVSQSKVE
                     VDAGLRAIWQGYAFATDFREERGHAYMLEDQQYTQRQVVAKQPGTCLNCHASMYVAYK
                     KAGNDDIVKGFEKVNSLPYAEAAKLVKHPVACIDCHDSATFALRVTRPAFVEGMRAFK
                     ASQGVPNYDVNKHAKPNEMRAYVCGQCHVEYYFKGPEKRLVYPWAKGLKVDQIAAYYD
                     EIGFRDWTHKDTGAPALKAQHPEFEMWNQGVHGRSGVTCTDCHMPMTTYKGQRLTDHW
                     VRSPVLNLKDACVSCHKKHDDKITEAQLKERVEDIQDRHWALREKAMAAVVGLIDDLK
                     AAKAAGRGDADLRTARYLQRRAQFYLDFVEAENSTGFHAPQEGARILGESIDFARQGQ
                     VALRDKKFKPTIPVVDIPPPPAVPAPAK"
BASE COUNT          885 a         1455 c         1479 g          777 t
ORIGIN      
        1 gtatctgcga cgtggtatct gcgacgcctt atctgagacg agtctcgccg acaatgtcga
       61 gcgcgacggc ctccgcgact tcgatgccgt cgattgccgc ggaaagaatt ccccccgcgt
      121 agcccgcgcc ttcgcccgcg ggatagaggc cgcgcgtgtt gacgctctgg tagtcagtgc
      181 cgcgccggat gctgatggga gatgacgtgc gcgtctcgac gccggtcagc accgcatcct
      241 ccatcgcgta gccgcggatc tgccgctcga aggcgggcag ggcctcgcgt atcgccgcaa
      301 ccgcgtaacc ggggatgcag gtggagagat cggtccagcg cacgcccggc tggtaggacg
      361 gaaccaccgt cccctgcgcc gtcgaagggc gtcccgcgag aaaatcgccg acgcgctgcg
      421 cgggcgcgca gtaattgctg ccgcccgctt cgtaggcgcg ctcctcccag tgccgctgga
      481 aggcgatgcc ggcgagcggg tgcccgggat agtcggcggg cgtgatgccg acgacgatgc
      541 cgctgttggc gttcctctcg ttgcgcgagt actggctcat gccgttggtg acgacgcgcc
      601 ccggctccga ggccgccgca accaccgtac cgccgggaca catgcagaag ctgtagaccg
      661 agcggccgtt gccgcagtgg tatacaagct tgtagtcggc ggcgccgagc agggggtggc
      721 cggcgttcct gccgtagcgc gcgcggtcga tgagcgactg cgggtgctcg atgcgcaagc
      781 cgatggagaa gggcttcgcc tcgatgtgca cgccgcggtc gtgcagcatc tggaaggtgt
      841 cgcgcgcgct gtggccgacg gccagcacga cgtgatcggc ggcgatgcgc tcgccgtttt
      901 ccagtcccac gccgcgcacg cgctctccct cgatctcgac atccgtgacc ttgcattgaa
      961 accggatttc gccgccgagc gactggatgg tctcgcgcag gctctcgacc atggtcacca
     1021 gccggaacgt gccgatgtgc ggcttggcga cgtagagtat ttccggcggc gcgccggcct
     1081 ccacgaattc cgtgagcacc ttgcgcccga ggtggcgcgg gtccttcacc tggctgtaga
     1141 gcttgccgtc ggaaaaggtg ccggcgccgc cctcgccgaa ctgcacattg gattcggggt
     1201 tgagcaccga ttttcgccac agcccccagg tgtcctgggt gcgttcgcgc acgattttgc
     1261 cgcggtcgag gatgatgggg cggaaaccca tttgcgcgag cagcaggccg gcaaacagcc
     1321 cgcaggggcc ggtgccgatc accaccggcc gcgactcgag cgcggccggc gcctgcgcga
     1381 cgaactggta ggcggtattc ggcgttcggg tgatgcgccg gtcatccttc agccgcgcga
     1441 gcagagccgg ctcgttgctg acttctacgt ccagcgtgta agtgaacacg atggccggct
     1501 tgcgggcgtc cacggcccgg cggaagatgg tgtagccttc gagttcatcc gcgcgaatgc
     1561 ccagccgcct caggatagcg gcacggatgg cgtcctcggg atggtcgagc ggaagcctga
     1621 cttcggtgat gcgcagcatg ggcggtagtt atcctgccag ccgcaagtat aacgaaaggt
     1681 acaagtttca agttacaagt ttcaagataa accaggattc ccaagcgggg tttcttgcaa
     1741 cttgcgactt gtaacttata acttgtaact tgtaacttgt aacttgagac ttgcaactcg
     1801 cgaaagcgga ggctggaatg ttgaagggga aaaccgcgct ggtgaccggg tccaccagcg
     1861 gcatcgggct gggaatcgcg gaggcgttcg cgaaggaggg ggccagcatc gtgctgaacg
     1921 gtctggggcg cgccgacgag atcgaggcgc tgcgcgcgaa gatagccaaa gaccacaagg
     1981 tcgcggtgcg ctacgacggc gccgacatga cgcgccccaa caccatcgag tcgatgatgc
     2041 ggaaagcggt cgccgaattc ggtgcggtgg acatcctcgt caacaacgcc ggcatccagc
     2101 acgtcgcgcc gatcgacgaa ttcccggtgg agaagtggaa cgcgatcatc gcgatcaacc
     2161 tggtggcgtc gttccacacc atacgcctcg cgctgccggc aatgaagaag cgcaagtggg
     2221 ggcgcatcat caatatcgcc tcggcccatg ccctcgtcgc gtcgcccttc aagtcggcct
     2281 acgtcgccgc caagcacggc gtcgccgggc tcaccaagac cgtcgcgctg gaggtcgcgg
     2341 agcagggcat caccgtcaac gcgatctgcc cgggctacgt gcgcacgccg ctcgtcgaga
     2401 agcagatccc ggacacggcg aaggcgcgcg gcatcaccga ggagcaggtg gtgaaggacg
     2461 tgctgctcgc cgcgcagtcc accaagcagt tcgtcacggt tgggcaagtc gcctcgctcg
     2521 cccgctacct cgccagtgac gacgcggcgt ccatcaccgg cgccatcctg tcggtggatg
     2581 gtggatggac agcacactga agtcggaagg cgaaagtcga aaggcgaaaa cgcaaatcat
     2641 gaaaagaacg ttgattggcg ggtgttactt tcgactttcg actttcgcct tctgtccgag
     2701 acgtgaatgt tgtccctgac gatggggtag atctggttgt tccagtgctt gccgcagaat
     2761 acgttgcaac ttgatctgac ggacctggtc tgaacaagcc cgctatagag cgtttttgaa
     2821 gggacgctaa cggccaattg cagtcaatcg attagccaaa ccactcgctc cttcaccttc
     2881 ccgggcgacg cccgaattgt tcggttttcg cgcgccgccg ataaggaatt gtttatccca
     2941 catgatgaag gtcttgaccc aggagtaaac aatctggccc agatggatac catcgccgcc
     3001 gtcgtgctct ccgaggccct cgcgccgcgc tagtctcgag aacggaagcc ctcctcgccc
     3061 aatgcgcggt tccgggttac tattccatgg cgccgcgcga aagcagctct cgtcacttga
     3121 actcggttcg gccggccaac gttggccttg gggagagaaa catggacagt gcatcgaaat
     3181 cccgctcgcg catcctggcg gccacggggt gggcggtcct cgtcaccggg ctcctgctga
     3241 tctccgcgac ggtgattccc gccgacgacg cgaagaaagg cgccccccca acgatcaccg
     3301 acagcaccga cgatccggcc gtctggggga aaacgttccc cctgcactac gagctctaca
     3361 agaagtccac cgacatgaca cgcacgaagc atggcggcag cgaggcgatg ccgcacaagc
     3421 caacccccgg cgatccgcgc tcgaaggtct cacaatccaa ggttgaggtg gatgcgggcc
     3481 tgagggcaat ctggcaaggc tatgcgttcg ccacggattt ccgcgaggag cgtggtcacg
     3541 cctacatgct cgaagaccag cagtatacgc agcgccaggt ggtcgcgaaa cagcccggga
     3601 catgcctgaa ctgccacgcc tctatgtacg tcgcgtataa gaaggccggc aacgacgaca
     3661 tcgtcaaggg cttcgagaag gtgaactccc tgccttacgc cgaggcggcg aagctggtga
     3721 agcatccggt cgcctgtatt gactgccacg acagcgcgac gttcgcgctt cgagtcacgc
     3781 gccccgcgtt cgtggagggc atgcgcgcgt tcaaggcctc gcagggcgtg ccgaactacg
     3841 acgtgaacaa gcatgccaag cccaacgaga tgcgtgccta cgtgtgcggc caatgccacg
     3901 tcgagtacta cttcaagggc cccgagaaac ggctcgtgta tccctgggcg aaaggtctca
     3961 aggtcgatca gatcgctgcc tactacgacg agatcggctt tcgcgactgg actcacaagg
     4021 acaccggcgc cccggcgctg aaagcgcaac acccggagtt cgaaatgtgg aaccagggcg
     4081 tgcacggtcg ctccggcgtc acgtgcaccg actgccacat gccgatgacg acctacaagg
     4141 gccagcgcct caccgaccat tgggtgcgca gcccagtcct caacctcaag gacgcgtgcg
     4201 tcagctgcca caagaaacat gacgataaaa tcaccgaggc gcaactcaaa gagcgcgtgg
     4261 aggatatcca ggatcggcat tgggcattgc gcgagaaagc catggccgcc gtggtgggac
     4321 tgatcgacga cctcaaggcg gcgaaggccg ccggacgcgg cgatgcggac ctgcgcacgg
     4381 cccgctacct gcagcgccgc gcgcagttct acctcgactt tgtagaagcg gaaaactcca
     4441 ccggcttcca cgcgcctcag gaaggggcgc gcatcctcgg cgaatcgatc gacttcgcgc
     4501 gccagggcca ggtcgccttg cgcgacaaga aattcaagcc gacgataccc gtggtcgaca
     4561 tccctccgcc tccggcggtg ccggcgccgg cgaaga
//
LOCUS       DBNGSG010000053         4662 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4600276, whole genome shotgun sequence.
ACCESSION   DBNGSG010000053 DBNGSG010000000
VERSION     DBNGSG010000053.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4662)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4662)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4662
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4600276"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..1284)
                     /locus_tag="AC158Q_01195"
     CDS             complement(61..1284)
                     /locus_tag="AC158Q_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR3338413.1"
                     /translation="MAGPLSHIRVLDLSRVLAGPWCGQNLADLGAEVIKIERPKTGDD
                     SRAFGPPWLKDRDGKDTRESAYFASANRGKKSVTVDLSKPEGQEIVRGLARQSDVLIE
                     NYKVGDLARYGLGYDDLSRPNPRLVYASVTGFGQTGPYRERPGYDFMIQGMGGVMSIT
                     GERDDRPGGGPQRVGIPIADIMTGMYATIAICAAIAHRERSGAGQHLDLALLDTQVGI
                     LANQGMNYLATGVAPGRIGNAHPNIVPYQPFRTRDGDVILACGNDKLFNKFCEVAGCG
                     ELVKDPRFATNSKRVENREAITALLAEIFARRTTRDWCDALERAGVPNGPINDLKQVF
                     DEPQVAARGMKIELEHAVAGKVPLIASPMKFSDTALEFKNPPPVLGQHTDEVLRGALR
                     MDDGAIAKLRADGII"
     gene            complement(1475..2284)
                     /locus_tag="AC158Q_01200"
     CDS             complement(1475..2284)
                     /locus_tag="AC158Q_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR3338414.1"
                     /translation="MNADNIKTGTPAVLTERDGAIATVTLSNPGKLNALTVSMWQELA
                     RAMDALSADDSLRCIVLRGAGSEAFAAGADIAEFASARDNARQGKRYHREYVHGALKA
                     VGECRHPTVAMIHGPCVGGGLEIACMCDLRISGVSGRFGVPISRLGFPIAYDELAAVL
                     PLTGRAVALEILVEGRVWEAEEAAAKGLLTRVLPDARVGEEAYACARRIAEGAPLANR
                     WHKQFVRRLAPRPAPLTEAEIEENFAYFATEDYRIGMQAFAEKRKPRFKGR"
     gene            2606..3568
                     /locus_tag="AC158Q_01205"
     CDS             2606..3568
                     /locus_tag="AC158Q_01205"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3338415.1"
                     /translation="MIARWTAAIALSLAALSGHAQDYPTRMINITVPNPPGGMNQIHA
                     QPLSAVIEQITKQPAPIVNRPGGTAGIGTAYVANQPPDGYNILVTTANLHLAIEKDKL
                     YGIKSPYTLEQIAPLALMSADPLIMVIHPSMPVKNVKELVALAKSKPNEIVFSSSGPY
                     GITHTPTAMFLHAAGIKMRHLPTTGGGPALTQALGGHSQVTGGGPATTYPLVASGKLR
                     AVASWGVKPHPVFPDTPTFQSMGYKNVEAYLWVGLFTRTGIPEPVFNKMREMIAKVTA
                     DPAFKKALEAVKVVPDYRDAPEFRKFFEADHKRMAAAIKNIGKL"
     gene            3738..>4662
                     /locus_tag="AC158Q_01210"
     CDS             3738..>4662
                     /locus_tag="AC158Q_01210"
                     /inference="COORDINATES: protein motif:HMM:NF024771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR3338416.1"
                     /translation="MKIKRIEPIAVSLPMVKPLKMAFEEVKSAENVLVRLETDEGAVG
                     WGGAGAAPTMTGETVASMAAAIRYLAPRLEGMALDDITAVMARAGNYLFGNQSAKSVI
                     EIALHDALGRARGKPAHDLLGEKRRDRVAVLRMVGTGKGTAADIEDAQHGRAEGYVAF
                     KNKVGVADPREDAERTRAICEALSGNGTRLICADANQGWTPDQAVAYVRAVEDTTLAF
                     FEQPVDGHDVEGMAKVARASRVKIGCDEGLHSLEDLKRHHETGAAAGFSLKTIKLGGM
                     RPVMDAGVLCDRLGLKVNLASKMAETGICTAA"
BASE COUNT          791 a         1601 c         1516 g          754 t
ORIGIN      
        1 aactgccgaa agggtcagga aaagcgtgac ggctggcggc tggcggctgg cggctggcgg
       61 ctagatgata ccgtccgccc gcagcttcgc gatggctcca tcgtccatgc gcagcgcgcc
      121 gcgcagcacc tcgtcggtgt gctgtcccag gaccggcggc gggttcttga actcgagcgc
      181 ggtgtccgag aacttcatcg ggctcgcgat cagcggcact ttgcccgcga ccgcgtgctc
      241 gagctcgatc ttcatgccgc gcgcggcgac ctggggctcg tcgaacacct gcttcaggtc
      301 gttgatcggg ccgttgggca cgcccgcccg ctcgagcgcg tcgcaccaat ccctggtggt
      361 gcgccgcgcg aaaatctccg cgagcagggc ggtgatcgcc tcgcggtttt ccacccgctt
      421 cgaattggtc gcgaagcgcg ggtctttgac gagttccccg cagccggcga cctcgcagaa
      481 cttgttgaag agtttgtcgt tgccgcaggc gaggatcacg tcgccgtccc gggtccggaa
      541 cggctggtac ggcacgatat tcgggtgcgc gttgccgatg cgtcccgggg caacgccggt
      601 cgcgaggtag ttcatgccct ggttggcgag gatgccgacc tgggtgtcga gcagcgcgag
      661 gtcgaggtgc tggcccgcgc cgcttctctc gcggtgcgcg atcgccgcgc agatcgcgat
      721 cgttgcgtac atgccggtca tgatgtcggc gatcgggatg cccacgcgct gcggcccgcc
      781 gcccggccga tcatcgcgct cgccggtgat gctcatcacc ccgcccatgc cctggatcat
      841 gaagtcgtag ccggggcgct cgcggtacgg cccggtctgg ccgaacccgg tcaccgaggc
      901 atagaccagc cgcgggttgg gcctgctcag gtcatcgtag cccaggccgt agcgcgcgag
      961 gtcccccacc ttgtagttct cgatcaggac gtccgactgc cgcgcgaggc cgcgcacgat
     1021 ctcctggcct tcgggcttcg agaggtcgac ggtcaccgac ttcttgccgc gattggcgga
     1081 ggcgaagtag gccgactccc gggtgtcctt gccgtccctg tccttcagcc acggcgggcc
     1141 gaaggcgcgc gagtcgtcgc cggttttcgg ccgctcgatc ttgatcacct cggcgccgag
     1201 atcagcgagg ttctgcccgc accacggccc ggcgagcacg cgcgacaggt ccaggactct
     1261 gatatgcgaa agcggtccgg ccatcgttct accttcttta tcgaaaatcg tttcggatac
     1321 tgcgtctcag gtactgcgtc tcagatactg cgtctttacg catcatctgc gacgccttat
     1381 ctgcgacgcc ttatctgcaa cgcattatct gcgacgcctc atccgggacg ccctatctgc
     1441 gacgcttcat ccgggacgcc gcgcccgcgg cgtcctagcg ccccttgaag cgcggcttgc
     1501 gcttctccgc aaacgcctgc atgccgatgc ggtagtcctc ggtggcgaag taggcgaagt
     1561 tctcctcgat ctcggcctca gtgagcggag cgggccgcgg cgcgagccgg cgcacgaact
     1621 gcttgtgcca gcggttggcg agcggcgcgc cttccgcaat gcggcgcgcg caggcatagg
     1681 cttcttcccc tactctggcg tcgggcaaga cgcgcgtcaa caatcctttc gcggccgctt
     1741 cctccgcctc ccacacccgg ccttccacca ggatctccag cgccaccgcg cgcccggtga
     1801 gcggcagcac ggccgccagc tcgtcgtagg cgatcggaaa gcccaggcgg ctgatcggca
     1861 cgccgaaacg ccccgagacg ccggaaatcc gcaggtcgca catgcacgcg atctccaggc
     1921 cgccgcccac gcacgggccg tgaatcatcg ccaccgtcgg atggcggcac tcgccgacgg
     1981 ctttcagcgc gccgtgcacg tattcgcggt gatagcgctt gccctgccgc gcattgtcgc
     2041 gcgcgctggc gaactcggcg atgtcggcgc ccgcggcgaa tgcctcgctt cccgccccgc
     2101 gcagcacgat gcagcgcagc gaatcgtccg ccgacagcgc gtccatcgcg cgcgccagct
     2161 cctgccacat ggacacggtc agcgcgttca gcttgcccgg gttgctcagc gtcaccgtcg
     2221 caatagcgcc gtcgcgctcg gtcaggactg cgggagtgcc ggtcttgatg ttatctgcgt
     2281 tcatcggcgt tcatcccgtt ccaatgacca ccaccaatta ctatttatat tgacagtccg
     2341 gacaccccgg gaagacggac attggaacgg gatttattgg cggctgcaat tctagccgat
     2401 cgccgtgcgc aggcacgtca acgcgaccag cgtcccgcca gccgcgctca cgcccgcgaa
     2461 atgaccgggc cggtccgggg ccagacacgc tccggacttc atattaccgt cgaattcttg
     2521 ccaaaccacg tgatgtcggg ttaacgttcc acgggttgga cccgtgcggc cgtgtggggc
     2581 cgcgtcacaa cgaggaggga aaatcatgat cgccagatgg acggcagcaa tcgccctgtc
     2641 gctcgccgcg ctaagcggcc acgcgcagga ctacccgaca cgcatgatca acatcaccgt
     2701 gccgaacccg ccgggcggaa tgaaccagat tcacgcgcag ccgctctccg cggtgatcga
     2761 gcagatcacc aagcagccgg cgcctatcgt caaccggccc gggggcaccg ccgggatcgg
     2821 caccgcctac gtcgccaacc agccgcccga cggctacaac atcctggtga cgacggcgaa
     2881 cttgcacctg gcgatcgaga aggacaagtt gtacggcatc aagtcgcctt acacactcga
     2941 gcagatcgcg ccgctggccc tgatgagcgc cgatcccctg atcatggtca tccatccttc
     3001 gatgcccgtg aagaacgtga aagagctggt cgcgctcgcc aagtcgaagc cgaacgagat
     3061 tgtcttctcg tcctcgggcc cctacggcat cacgcatacc cccacggcga tgttcctcca
     3121 tgccgcgggg atcaagatgc ggcatctgcc gaccaccggc ggcggccccg cgctgaccca
     3181 ggcgctcggc gggcactcgc aggtaaccgg cggcggcccg gcgacgactt atccgctcgt
     3241 cgcgtcgggg aagctgcgcg ccgtcgccag ctggggcgtc aagccccacc cggtgttccc
     3301 cgacaccccg acgttccagt cgatgggcta caagaacgtg gaagcgtacc tctgggtcgg
     3361 gctgttcacg aggaccggga ttcccgagcc ggtgttcaac aagatgcgcg agatgatcgc
     3421 caaggtaacc gccgacccgg cgttcaagaa agcgctcgag gcggtcaagg tcgtgcctga
     3481 ttaccgcgac gcgccggagt tcaggaagtt cttcgaggcc gatcacaagc gcatggcggc
     3541 agcgatcaag aatatcggca agctttagcg caaaggcgtc gcagatacta cgtcgcaggt
     3601 gaggcgtccc agataaggcg tcgcagaaga agcgtcgcag atagggggtc gcagatgatg
     3661 cgtatagacg cagtacctga gacgcgctac ctgagacgca ctatctggga cgttttacct
     3721 gagacgcctt taccaacatg aaaatcaagc gcatcgagcc catcgcggtc agcctgccga
     3781 tggtgaagcc gctcaagatg gcgttcgagg aggtgaagag cgccgagaac gtgctggtgc
     3841 ggctcgaaac cgacgagggc gccgtcggct ggggcggcgc gggcgccgcc cccaccatga
     3901 ccggcgagac ggtggcgagc atggcggcgg ccatccgcta cctcgcgccc cggctcgagg
     3961 gcatggcgct cgacgacatc acggccgtca tggcgcgcgc cggcaactat ctcttcggca
     4021 accagtccgc caagtcggtg atcgaaatcg cgctgcacga cgcgctcggc cgcgccaggg
     4081 gcaagcccgc ccacgacctg ctcggcgaga agcggcgcga ccgggtggca gtcctgcgca
     4141 tggtcggcac cggcaagggc acggccgccg acatcgagga cgcgcagcac ggccgggccg
     4201 aaggctacgt cgccttcaag aacaaggtcg gcgtcgccga cccgcgcgag gacgccgagc
     4261 gcacccgcgc catctgcgag gcgctttccg gcaacggcac ccggttgatc tgcgccgacg
     4321 ccaaccaggg ctggacgccc gaccaggcgg tcgcctacgt gcgcgcggtc gaggacacga
     4381 cgctcgcctt cttcgagcag ccggtggacg ggcacgacgt cgagggcatg gcgaaagtgg
     4441 cgcgcgcgag ccgcgtcaag atcggctgcg acgaggggct gcattccctc gaggatctca
     4501 agcgccacca tgagaccggc gccgccgccg ggttcagcct gaagaccatc aagctgggcg
     4561 gcatgcggcc cgtcatggac gcgggcgtgc tgtgcgaccg gctcgggctg aaggtcaacc
     4621 tcgcctccaa gatggcggaa accggcatct gcaccgcggc gc
//
LOCUS       DBNGSG010000054         8147 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_355092, whole genome shotgun sequence.
ACCESSION   DBNGSG010000054 DBNGSG010000000
VERSION     DBNGSG010000054.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 8147)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8147)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8147
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_355092"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            318..1508
                     /locus_tag="AC158Q_01215"
     CDS             318..1508
                     /locus_tag="AC158Q_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014896040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HIR3338417.1"
                     /translation="MQPLAISRYSIVNCLGAGARAIAEALRDRRSGLRLCDFETVALD
                     TWIGRVPDLESLPVLPGLSAYDCRNNRLAQLGLELDGFSGAVAAAVRRYGAERIGVFM
                     GTSTSGILETELAYRRRDPRTGALPGDYRYTGTHNTFSLADFVRCYLGLRGPAFVVSS
                     ACSSSAKVFGNAARMIAAGICDAAVVGGVDTLCLTTLHGFQSLELTSRGPCRPFDEER
                     DGISIGEGAGFALIERTAQANAGRSLLLGVGESCDAHHMSTPHPEGLGAKLAVQGALD
                     SAGLAPADIDYINLHGTATRTNDAAEDRAVCELFGTGTACSSTKGATGHLLGAAGITE
                     AVISLIAIEQGLLPGSANTRRLDPALRSHYLLDNREARVRRVLTNSFGFGGTNCSLVL
                     GTAD"
     gene            1508..2305
                     /locus_tag="AC158Q_01220"
     CDS             1508..2305
                     /locus_tag="AC158Q_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014618004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HIR3338418.1"
                     /translation="MRAYVEGVGLCGPGLGGWEASRALLAGDAPYQPGPTAVTASELL
                     PAAERRRVGMPVKLALAAGREALAAAARDAAGMATVFASSSGDCDNVHHMLEALAAPE
                     RQISPTRFHNSVHNAAAGYWSIATRCRAPSTSLACRDASFAAGLLEAACQVAGDGAAV
                     ALIAYDHPYPEPLHAVRTIPAAFGIALVLAPDATDRSIAALDIAFVPGETAATPVAVP
                     GLEALGNDVPAARSLPLLTLLARGSPGTVDLEYVSGTHLRVVVAPCA"
     gene            2296..2772
                     /locus_tag="AC158Q_01225"
     CDS             2296..2772
                     /locus_tag="AC158Q_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006481514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog family protein"
                     /protein_id="HIR3338419.1"
                     /translation="MRLSRAEIAGMIPHAGAMCLLDGVVSWDENRIRCVSRSHLDTGN
                     PLRAGGRLPAVCGLEYAAQAMAVHGGLAGGARARPRAGYLASVRNMVCRRERLDDLEA
                     DLVVEAERVMGDEDRVIYEFRLWVGDAEVLSGRAAVVLDVNSIKPLMNGDRSTSNP"
     gene            2945..3664
                     /gene="fabG"
                     /locus_tag="AC158Q_01230"
     CDS             2945..3664
                     /gene="fabG"
                     /locus_tag="AC158Q_01230"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HIR3338420.1"
                     /translation="MKRALVTGGSGSIGAAICRRLAADGLHVTVHANSRLKEAQQLAG
                     EIASAGGNAQAVAFDATDRAQTGAALEKLLAGGAIQVVVNNAGIHDDAVMPAMKPEQW
                     SRVIDVSLNGFFNVTQPLLLPMMATRWGRIVNVTSVAGLTGNRGQVNYAAAKAGLHGA
                     TKSLAIELASRGITVNAVAPGIIATAMSRDAFPRETIERLVPMKRAGTPEEVAGLVGF
                     LASDAAGYISGQIISVNGAMI"
     gene            complement(3852..4634)
                     /locus_tag="AC158Q_01235"
     CDS             complement(3852..4634)
                     /locus_tag="AC158Q_01235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3338421.1"
                     /translation="MSASSSSHLVLIPSYNTGGKVLQTVWEAKRAWQPVWVVVDGSND
                     GTLEALQALARDDPDIRVLALPRNQGKGAAILHGLREAAALGYTHVLAMDADGQHPET
                     HIREFMDASLANPDAQILGVPVFDHTAPRERVHARKIVNWWANLETLWEGIGDSLFGF
                     RVYPVGPLLEIMERQPWMRGYDFDSEAAIRLCWRGIYPINIAVPVRYFRPEEGGVSHF
                     NYLRDNVILFCMHLRLLVESLLRMPFLLQRRLNGIKPANGQD"
     gene            complement(4640..5518)
                     /locus_tag="AC158Q_01240"
     CDS             complement(4640..5518)
                     /locus_tag="AC158Q_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HIR3338422.1"
                     /translation="MPKNVNGAMRSALYSRAVALFHAVLVCAGAAACARAEQLPLWEA
                     GMGAAALSFPDYRGSDERQFWLLPFPYLVYRGEFLQADERRMRGLFFKTDRLEVDVSF
                     NGSVPVDSSKNDARRGMPDLDATLEIGPALNVLLMQSDDRKTRVELRLPVRAVLASDF
                     SYVRHVGWIFQPNLSVDVRDPLGNAGWNLGLLAGPLVTDRRYNRYFYAVEPAFATATR
                     PAYSPGGGYAGTQVIAALSKRYRQFWVGGFVKWDTINGAAFADSPLVRDRQGVAAGFS
                     IAWILGESKTLVETTR"
     gene            complement(5503..6582)
                     /locus_tag="AC158Q_01245"
     CDS             complement(5503..6582)
                     /locus_tag="AC158Q_01245"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR3338423.1"
                     /translation="MAPTENHFGKDSRTAYDALSEAQRIAFAPIVFQACRVLRNSGVL
                     ELVRQSGAAGLSLNEVAAGIGLPRYGIKVLLESGAGIGLFCLNDGRYALTKLGYFMLS
                     DPMTRINMDVVHEVCYRGMFDLDRAVREGKPVGLKTLGSWKTFYEGLSSLPSRARESW
                     LAFDHYYSDMAFPAALSLVFADHPGRLLDVGGNTGKWAVQCAQFDPDVRVTIADLPQQ
                     LTLARETIRENRLDDRVDFLAIDVLDESRPFPEGYDAIWMSQFLDCFSENEIVSILRR
                     AARSLPASGHLYILELFWDRQLNETAAFCLQQTSLYFTCIANGNSQMYHSADMLRCLE
                     ESGLELAEQRDQVGLYHTLLKCRKT"
     gene            complement(6600..7922)
                     /locus_tag="AC158Q_01250"
     CDS             complement(6600..7922)
                     /locus_tag="AC158Q_01250"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3338424.1"
                     /translation="MSDTAQTTESRCDVLVIGGGPAGSTIAALLAERGRDVVLLEKER
                     HPRFHIGESLLPLNMPLFDRLGVGEEIRAIGMPKYGAEFVSQWHDEPVHVDFANAWDC
                     VSPSTYQVRRSEFDQILFRNAARKGAKTVEGCRVTGVDFHDDGALVTARRDDGMEQRW
                     QARFVADASGRDTFLANRLGIKQRNKKNNTAALFGHFSGAKRLPGKIEGNISLFWFEH
                     GWFWFIPLSDGATSIGAVCSPQYMKSRKTDPRQFFLDTIALCPVLAERLRNATLVSPV
                     TATGNYSYMAERTGGRNYLLLGDAFAFVDPVFSTGVLLAMESGLAGADTIETCLDRPG
                     DASSALRRFDDRTRHGLRVFSWFIYRVMTPSLRFLLMNPTQRFHMQPAILSLLAGDVF
                     RGTPVGLRLFVFKFFYYLFSVLALRKSLMAWRERKRAVRESGAGTAAT"
BASE COUNT         1485 a         2549 c         2666 g         1447 t
ORIGIN      
        1 gcgcggccga gtggacgcgc ctgctggaag cgctcggctt ttcagtcgcc gtcgagccga
       61 tgagcgcggg aacaccgttt gccaacgtgc tctacatcgc caaaaaaata gacaggatta
      121 acaagatttg acaggattta caaatttaac aaacactcga tatgagctcc gattcgacgt
      181 cttgtcgcgc tgatcagcct tgtcaatcct gtcaaggtgc ataacgagac ttacgggaac
      241 gagatcaata atgtttgcct tgcttctacc ctgttaatct tgtgaaatct tgttaatcct
      301 gtccattttt tgatctcgtg caaccgctcg ccatcagccg ctactcgatc gtgaattgcc
      361 tcggcgccgg ggcgcgcgcg atcgcagagg ctttgcgcga caggcgatcg ggcctgaggc
      421 tctgcgattt cgagaccgtt gcgctcgata cctggatcgg gcgggttcct gatctggaat
      481 cgctgccggt gttgcccggc ctgtctgcct atgactgccg caacaaccgg ctcgcgcaac
      541 tggggctcga gctggacggc ttttccggtg ccgttgccgc cgcggtgagg cgctacggcg
      601 cggagcggat cggcgtgttc atgggcacca gcacgtcggg gatactcgag acggagctgg
      661 cttatcgccg ccgtgatccg aggacgggcg cactgccggg ggactatcgc tacaccggga
      721 cacacaatac cttctcgctg gcggatttcg tgcggtgcta cctcggcctg cgcggcccgg
      781 ccttcgtcgt gtcctccgcg tgttcctcca gcgccaaggt gttcggcaat gcggcacgca
      841 tgatcgccgc cgggatctgc gatgcggcgg tggtgggtgg cgtggacaca ctgtgcctca
      901 ccacgctcca tggctttcaa tcgctggaac tgacgtcgcg cgggccctgc cgcccgttcg
      961 acgaggagcg ggacggcatt tccataggcg agggcgccgg ctttgccctg atcgagcgca
     1021 ccgcgcaggc gaatgccggc cgctcgctgc tgctgggggt gggggagtcc tgcgacgccc
     1081 accacatgtc cacgccccat cccgaggggc tgggcgcgaa actcgccgtg cagggcgcgc
     1141 tggacagtgc ggggctcgcg ccggcggaca tcgattacat caatctccac ggtaccgcaa
     1201 cgcgcaccaa cgacgcggcc gaggaccgcg cggtctgcga actgttcggc acgggcacgg
     1261 cgtgcagctc gaccaagggc gcgaccggcc acctgctcgg ggcggccggg atcacggaag
     1321 cggtcatatc tctgatcgcg atcgagcagg gcctgctgcc gggcagcgcg aacacgcgcc
     1381 gtctcgaccc cgcattgcgc agccactacc tgctcgacaa ccgagaagcg cgcgtgcgcc
     1441 gggtgctcac caactccttc gggttcgggg gcaccaactg cagcctcgtg ctgggaacgg
     1501 cggattgatg cgcgcctatg tcgaaggggt ggggctgtgc gggccgggcc tgggcggatg
     1561 ggaagccagc cgggcactgc tcgcgggcga cgcgccgtac caacccgggc ccactgccgt
     1621 tacggcgagc gagctgctgc ccgccgcaga gcggcgccgc gtcggcatgc cggtcaagct
     1681 cgccctggca gcggggcgcg aagctcttgc cgccgccgcc cgcgacgccg caggaatggc
     1741 gaccgtgttc gcttcatcga gcggcgattg cgacaacgtg caccacatgc tcgaagcgct
     1801 tgccgcgccg gaacgccaga tctcaccgac ccgttttcac aattccgtgc ataacgcggc
     1861 cgccgggtac tggagcattg cgacccggtg tcgggccccg tccaccagcc tcgcatgccg
     1921 cgatgcgagc tttgccgcgg gcctgctgga agccgcgtgc caggtggcgg gcgacggcgc
     1981 agcggtcgcg ctgatcgcct acgatcaccc gtaccccgag cctttgcacg ccgtgcggac
     2041 catccccgcg gctttcggca tcgcgctggt gctggccccg gatgcgacgg accgcagcat
     2101 tgccgcattg gatatcgcgt tcgttcccgg ggaaacggcc gcaaccccgg tggcggttcc
     2161 cgggctcgaa gcgctcggga acgatgtccc cgcggcgcgc agcctgccgc tgctgacgct
     2221 gctggcgcgc ggctcccccg ggacagtgga tctcgagtac gtttccggga cgcaccttcg
     2281 tgtggtggtg gccccatgcg cctgagccgg gcggagatcg ccggaatgat cccccacgcc
     2341 ggggccatgt gcctgctgga tggggtcgtg agctgggacg aaaaccggat acgctgcgtg
     2401 agccgcagcc acctcgacac cggcaacccg ctgcgcgccg gcgggcggtt gccggcggtg
     2461 tgcggtctcg aatacgctgc gcaggcgatg gccgtgcacg gcgggctggc cggcggggcc
     2521 cgcgccaggc cgcgcgccgg ctatctggcg agcgtgagga acatggtgtg ccggcgcgag
     2581 cggctggacg acctcgaagc tgacctggtg gtcgaggccg aacgggttat gggtgacgag
     2641 gatcgcgtga tctacgagtt caggctgtgg gtgggagacg cggaagtgct gagcggacgc
     2701 gcggcggtgg tgctcgacgt taattccata aagccgctga tgaacggcga tcgatcaact
     2761 tcaaacccat gaaaccgccg atgaacgccg atcaacgccg ataaattcag gacaaaagta
     2821 tgcacgtcgg gagtaacaaa gccatgccgc tggttgtcag aagggctttt cttgtatttt
     2881 ccgcgtcgaa gctttgttcg gttcctgatc ggcgtgtatc ggcgttcatc ggcggcggat
     2941 tgaaatgaaa cgggcgctcg tcaccggcgg cagcggcagc atcggcgcgg ccatctgccg
     3001 gcggcttgcc gccgatggcc tgcacgtcac ggtgcatgcc aacagccgcc tgaaggaggc
     3061 gcagcaactg gctggcgaga tcgcctccgc cggcggaaac gcgcaggccg tggctttcga
     3121 cgcgaccgac cgcgctcaga ccggcgccgc gctggaaaaa ctgctggcgg gcggggctat
     3181 ccaggtcgtg gtcaataacg cggggataca tgacgacgcc gtaatgccgg cgatgaagcc
     3241 ggagcaatgg tcacgcgtga tcgacgtgtc gctgaacggc ttcttcaacg tgacgcaacc
     3301 gctgctcctg ccgatgatgg ccacgcgctg gggccgcatc gtcaacgtga cctcggtcgc
     3361 gggactgacc ggcaaccgcg gccaggtcaa ttacgcggcg gccaaggccg gactgcacgg
     3421 cgcgaccaag tcgctcgcga tcgagctcgc cagccgcggc atcaccgtga acgccgtcgc
     3481 gcctggcatc atcgccacgg ccatgagccg ggatgcgttt ccccgggaga cgatcgagcg
     3541 gctggtgccg atgaagcgcg ccgggacgcc ggaagaagtg gcgggcctgg tgggatttct
     3601 cgcttcggac gcggccggct atatctcggg gcagatcatc tccgtcaacg gcgcgatgat
     3661 ctgaaccacg atgaatggac aggattgacg gaatcgaaaa cccgtcagaa tccttaatag
     3721 acaggattaa caagattaac aggattaaga aaagatccga actgcagttt ggtttttgtc
     3781 ttcgatccaa tcttgtgaat cctgttaatc atgtccattt acggggttga cgggtttgcg
     3841 atgtatcgct gttaatcctg tccgttcgcg ggttttatgc cgttcagccg gcgttgcagc
     3901 aggaacggca tccgcagcag cgactccacg agcagccgca ggtgcatgca gaacaggatc
     3961 acgttgtcgc gcaggtaatt gaaatgcgaa acaccgcctt cttcggggcg gaaatacctg
     4021 accggcacgg cgatgttgat cggatagatg ccgcgccagc acaggcggat ggcggcctcg
     4081 gagtcgaaat cgtagccccg catccagggc tgccgttcca tgatctcgag cagcggcccg
     4141 accggataga cgcggaaacc gaagagcgaa tcgccgatcc cttcccagag ggtttccagg
     4201 ttcgcccacc agttgacgat tttccgggcg tgcacgcgct ccctcggcgc ggtgtgatcg
     4261 aacaccggca cgcccagtat ctgcgcgtcg ggatttgcca gggaggcgtc catgaactcg
     4321 cggatatgcg tctccgggtg ctgtccgtcg gcgtccatcg ccagcacgtg cgtatagccg
     4381 agcgccgcgg cttcgcgcag gccgtgcagg atcgcggcgc cttttccctg gttgcgcggc
     4441 agcgccagga ccctgatatc cggatcgtca cgcgccagcg cctgcagcgc ttccagcgtg
     4501 ccgtcgttgc tgccgtccac gaccacccac accggctgcc aggcgcgctt cgcttcccac
     4561 acggtctgca ggaccttgcc accggtgttg tagctcggga tgagcacgag gtgcgaggag
     4621 gatgaagcgc tcatgttttt tatcgtgtcg tttcaaccag cgttttggat tcgccgagta
     4681 tccaggcgat ggaaaagccc gccgccacgc cctgcctgtc cctgaccagc gggctgtccg
     4741 cgaatgccgc gccgtttatc gtatcccatt ttacgaagcc gccgacccag aattgccggt
     4801 agcgcttgct caacgcggcg atcacctggg tgccggcata ccctcctccc gggctgtagg
     4861 cggggcgggt ggcggtagcg aacgccggtt cgactgcgta gaaataccgg ttgtagcgcc
     4921 ggtcggtaac cagcgggccg gcgagcagac cgaggttcca gcccgcgttg cccagcgggt
     4981 cccggacgtc gacgctcagg ttcggctgga agatccagcc cacgtgcctg acgtacgaaa
     5041 aatcagaggc gagcacggcg cgcaccggca ggcgcagctc cacgcgggtc ttgcgatcgt
     5101 ccgattgcat cagcaggaca ttcagcgccg ggccgatctc cagggtcgcg tcgagatcgg
     5161 gcattccccg ccgggcgtcg ttcttgctgc tgtcgaccgg caccgagccg ttgaaactga
     5221 cgtcgacctc gagccggtcg gtcttgaaaa acagcccgcg catccggcgc tcgtcggcct
     5281 gcaggaattc gccgcggtag acgaggtagg ggaatggcag cagccagaat tggcgctcgt
     5341 ccgagccgcg ataatccggg aagctcaacg cagcggcgcc catgccggct tcccacaggg
     5401 ggagctgttc cgcgcgggcg caggccgccg cgcccgcgca cacgaggaca gcatggaaca
     5461 gcgcaaccgc tcttgagtag agcgcggatc gcatcgcgcc attcacgttt ttcggcattt
     5521 gagcaaggtg tggtacaggc ctacctgatc gcgctgttcc gccaactcca gaccgctctc
     5581 ctccaggcag cgcaacatgt cggccgaatg atacatttgg ctgttgccgt tggcgatgca
     5641 ggtgaaatac agcgaggtct gttgcaggca gaaggcggcg gtttcgttga gctggcggtc
     5701 ccagaacagt tcgagaatat acaaatggcc gctggccggc agggaccggg cagcgcgccg
     5761 cagtatggaa acgatctcgt tctccgaaaa acaatccagg aactggctca tccagatggc
     5821 gtcgtaacct tccggaaacg ggcgggattc gtccagcacg tcgattgcga ggaaatccac
     5881 ccggtcatcc agccggttct cgcggatcgt ttcccgggca agggtcaact gttgcggcaa
     5941 atcggcaatg gtgactctca cgtccggatc gaattgcgcg cactggacag cccactttcc
     6001 ggtattgccg ccgacgtcca gcagccgccc cggatgatcg gcaaacacca gcgatagcgc
     6061 ggcaggaaac gccatgtcgg agtagtagtg atcaaaagcg agccagctct cccgcgcccg
     6121 ggacggcagg gaggacaggc cttcgtaaaa agttttccag ctccccaggg ttttcagtcc
     6181 gacgggcttg ccttcccgga ccgccctgtc cagatcgaac atgccgcgat agcacacttc
     6241 atgcaccaca tccatgttga tgcgcgtcat ggggtcgcta agcatgaaat agcccagttt
     6301 ggtcagcgca taacggccat cgttgaggca gaacagcccg atccccgcgc ccgattcaag
     6361 caggaccttg atcccgtagc gcggcaagcc gattccggcg gcgacttcat tcagcgacag
     6421 ccctgcggcg ccgctttgcc ggaccaattc cagaacaccg gagttgcgaa ggacgcggca
     6481 ggcttgaaac acgatcggcg caaaggctat tcgctgcgct tcggacaggg catcgtaggc
     6541 cgtcctcgaa tccttgccga agtgattctc cgtaggggcc atcacgaaca gtcatgaatt
     6601 caggtagcgg ctgtcccggc gccggattcc cgcacggcgc gcttgcgctc cctccatgcc
     6661 atcaggcttt tcctgagggc gagcacgctg aagaggtaat agaaaaactt gaacacgaac
     6721 agcctcaacc cgaccggcgt gccgcggaag acatcgcccg cgagcaatga aagtatggcg
     6781 ggctgcatgt ggaagcgctg ggtcgggttc atcagaagaa agcgcagcga gggcgtcatc
     6841 acccggtaga tgaaccagga gaacacccgc agcccgtggc gcgtcctgtc gtcgaagcgc
     6901 cggagcgcgc tgctcgcgtc gccggggcga tcgagacagg tctcgatcgt gtcggcgccg
     6961 gcgagcccgc tttccatggc aagcaggacg ccggtcgaga acaccgggtc cacgaaagca
     7021 aaggcgtcgc ccagcaggag gtagttccgc ccccctgtgc gctcggccat gtacgaatag
     7081 ttgccggtgg cggtgacggg agagaccagg gtggcgttgc gcagccgctc cgccagcacg
     7141 gggcagagcg cgatggtatc caggaaaaat tgccggggat cggttttgcg cgacttcatg
     7201 tactggggcg agcacaccgc gccgatgctg gtggcgccgt cggacagcgg gatgaaccag
     7261 aaccagccgt gctcgaacca gaacaggctg atgttgccct cgattttccc cggcagccgc
     7321 ttcgccccgg aaaagtggcc gaacagggcc gcggtgttgt tcttcttgtt gcgctgcttg
     7381 atgccgaggc ggttggcgag gaaggtgtcc cgtccggaag cgtcggccac gaaccgggcc
     7441 tgccatcgtt gttccatgcc gtcgtcccgt cgcgcggtca ccaaggcgcc gtcgtcatgg
     7501 aaatcgacgc cggtgacgcg gcagccttcg acggtctttg ctcccttgcg ggccgcgttg
     7561 cggaacagga tctggtcgaa ctccgagcgg cgcacctggt aggtggaggg agacacgcag
     7621 tcccaggcat tcgcgaagtc gacgtggacc ggctcgtcat gccactggga gacgaactcg
     7681 gcgccgtact tgggcatgcc gattgcccgg atttcctcgc ccaccccgag ccggtcgaag
     7741 agcggcatgt tgagcggtag cagggactcg ccgatgtgga agcgcgggtg ccgctccttt
     7801 tccagcagga cgacgtcgcg gccgcgttcc gcgagcaggg cggcgatcgt ggagccggcg
     7861 gggccgccgc caatcaccag cacgtcgcag cgggattccg tcgtttgtgc ggtatcggac
     7921 atcgtgaagc attatagaga aatccacccg tgcccggcgg gtaaaaaacc ttaaaagctc
     7981 tgacacaaag gacacgaagg acacaaagga aatctataca aagaacaaag aatcgatctt
     8041 ttagggttac gggatgcgca ataccgcagc ctccccattt attctggtgc ccctgactcc
     8101 aggattttcg tattaattat tttgtattcc tttgtgtcct tcgtgtc
//
LOCUS       DBNGSG010000055         2331 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4091953, whole genome shotgun sequence.
ACCESSION   DBNGSG010000055 DBNGSG010000000
VERSION     DBNGSG010000055.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4091953"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="AC158Q_01255"
     CDS             <1..228
                     /locus_tag="AC158Q_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR3338425.1"
                     /translation="PDVAPTVFVKGARYDKLIEAFGGVGVHATTPAELRKAMEEAVRS
                     RKPTLINAVIDETAGTESGRITSLNPSAKKK"
     gene            245..1516
                     /gene="frc"
                     /locus_tag="AC158Q_01260"
     CDS             245..1516
                     /gene="frc"
                     /locus_tag="AC158Q_01260"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HIR3338426.1"
                     /translation="MEALKGIRILDMTHVQAGPTCSQLLAWMGADVIKFENPQGDATR
                     GQLRDVPNADSLYFTMLNCNKRSITVNMKSAEGKKVFVDLLKKCDLIMENFGPGVLDR
                     FGFSWEKIHELNPKIVMGSIKGFGSTGPYSDFKAYENVAQAMGGAMSTTGVPEGPPFV
                     TGAQIGDSGTGLHLAIGLLAALRQAERTGQGQYVEVAMMDGVMNLCRVKFRDHQRLTR
                     QALAEYSVPTYQGMKDVPRAGNDSGGGQLGNAIHCKPHGANDWLYVVVQEAVWTALAN
                     RIGPDVGVPDLANDPRVATIAERRKNQNLVWTLLSKFAEKHTKRELMGILNPLDVPCG
                     PIMSTEDLATDEHVRGRDMWVELDHPQRGKWWNVGMPIKLSASPAVIKRSPTLGEHTE
                     EVLKEVLGYSEEQISKLKSAGAFSAPPKKAA"
     gene            1612..>2331
                     /locus_tag="AC158Q_01265"
     CDS             1612..>2331
                     /locus_tag="AC158Q_01265"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIR3338427.1"
                     /translation="MRIAIIGQQDYGKAVLEAFVARKDEVAGVFCAPEKPGAKADPLR
                     AAAEERGLKVFQFKSLREAEAHGALKSLGVDIGIMAYVLQFAPDTLVKIPRLGTIQYH
                     PSLLPKYRGPSSINWPIIRGDTRTGLTIFRPTEGLDEGPVVLQKECEIGPDETIGDVY
                     FNKLFPMGVKAMLEAADLVAAGKHKEVVQDESKASYEGWCREAEARVHWAHHVDFVYN
                     IIRGCNPAPGAWTTLNGKKLQI"
BASE COUNT          487 a          725 c          755 g          364 t
ORIGIN      
        1 cccgacgtgg cacccaccgt gttcgtcaag ggtgcgcgct atgacaagct gatcgaggct
       61 tttggtggcg tgggcgtgca cgccacgacc ccggccgaat tgcgcaaggc aatggaagaa
      121 gccgtccgct cgcgcaagcc cacgctcatc aatgccgtca tcgatgagac cgcgggcacc
      181 gaaagtggtc gcatcacgag cctgaatccg agcgcgaaaa agaaataggt ttttggagat
      241 cagaatggaa gcactcaagg gaatccgcat cctcgacatg acgcacgtgc aggcgggccc
      301 gacctgctcg cagctgctcg cctggatggg cgcggacgtg atcaagttcg agaatccgca
      361 gggcgacgcg acgcgcgggc agctgcgcga cgtgcccaac gcggacagcc tctatttcac
      421 gatgctcaac tgcaacaagc gctcgatcac ggtcaacatg aagagcgccg agggcaagaa
      481 ggtgttcgtc gatctcctga agaagtgcga cctcatcatg gagaatttcg gccccggcgt
      541 actggaccga ttcggtttca gctgggagaa gatccatgag cttaacccga agatcgtaat
      601 gggctcgatc aagggctttg gatccaccgg cccgtactcg gacttcaagg cctacgaaaa
      661 cgtggcgcag gcgatgggcg gggccatgag cacgaccggc gtgcccgagg gtccgccatt
      721 cgtcaccggc gcgcagatcg gcgattcggg taccggcctg cacctggcaa tcgggctgct
      781 ggcggcgctg cggcaggccg agcgcaccgg ccagggccag tacgtcgagg tcgcgatgat
      841 ggatggtgtg atgaacctgt gccgcgtgaa gttccgcgac caccagcggc tcacgcgcca
      901 ggcactcgcc gaatactcgg ttcccacgta tcagggcatg aaggacgtgc cgcgtgcagg
      961 taatgactcg ggcggcggcc agctcggcaa tgcgatccac tgcaaaccgc acggtgccaa
     1021 cgattggctc tacgtggtcg tgcaggaggc ggtctggacc gcgctcgcca accggatcgg
     1081 tcccgacgtg ggcgtaccgg acctcgcgaa cgacccgcgc gtggcgacca tcgccgagcg
     1141 ccgcaagaac cagaacctgg tatggacgct gctcagcaag tttgccgaga agcacaccaa
     1201 gcgcgaactc atggggatac tgaacccgct cgacgttccc tgcggcccga tcatgtccac
     1261 cgaagacctc gcgaccgatg agcacgtgcg cggccgcgac atgtgggtcg agctcgacca
     1321 cccgcagcgc ggcaaatggt ggaacgtcgg catgccgatc aagctgtccg catcaccggc
     1381 tgttatcaag cgctcgccga cgctcgggga acacaccgaa gaggttctga aggaagtcct
     1441 cggctacagc gaagaacaga tttcgaagct gaagagcgca ggcgcgtttt cggcgccacc
     1501 caagaaagcc gcttgagagt agcgcagccg cgcccgctca gttgccggcg cgttcgaacg
     1561 gacacggggt gccgttcaac gcgccgtttc gcttaacgac ggagacgacc aatgagaatt
     1621 gcgatcatcg ggcaacagga ctacggcaag gcagtactgg aagcgttcgt ggcgcgcaag
     1681 gacgaggtcg cgggcgtgtt ctgtgcgccg gagaagccgg gcgccaaggc cgatcccctg
     1741 cgggctgcag cggaggagag gggtctcaag gtcttccagt tcaagtcgct gcgcgaggcc
     1801 gaggcgcacg gggccctgaa aagcctcgga gtcgacatcg gcatcatggc ctacgtcctg
     1861 caattcgcgc ccgacaccct ggtgaaaatc ccccgcctag ggaccatcca gtatcacccg
     1921 tcgctgctgc ccaagtatcg cggcccttct tcgatcaact ggccgatcat ccgcggcgac
     1981 acgcgcaccg gcctcaccat cttccgccct accgaggggc ttgatgaggg gccggtcgtg
     2041 ctgcagaagg aatgcgaaat aggccccgat gagaccatcg gcgacgttta tttcaacaag
     2101 ctgttcccaa tgggcgtgaa agcgatgctc gaggccgccg acctcgtggc cgccggcaag
     2161 cacaaggaag tcgtgcagga cgagtcgaag gcgagctacg agggctggtg ccgcgaagcc
     2221 gaggcgcgcg tgcactgggc gcatcacgtc gatttcgtct acaacatcat ccgcggctgc
     2281 aaccccgcgc ccggcgcgtg gactacgttg aacggcaaga aactgcagat c
//
LOCUS       DBNGSG010000056         4532 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_186084, whole genome shotgun sequence.
ACCESSION   DBNGSG010000056 DBNGSG010000000
VERSION     DBNGSG010000056.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4532)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4532)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4532
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_186084"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /locus_tag="AC158Q_01270"
     CDS             complement(<1..1151)
                     /locus_tag="AC158Q_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HIR3338428.1"
                     /translation="MKRGQKWSLGLGIAAAVVGAAYLAVARWLPSDEELAARLSAEAE
                     ERLGVKVTIGSAHWTLLPRPAVVIRNFRTQQAQPVVIGQLTAHPGAGALLDRKLALRR
                     IAIRDAVFPRDSVRAFRGKPVAGKPGADGSLSLDHFVFANVTWISYSGIPVVYDGEVA
                     FDADWRPRRAELRRPGASPPFTLTAKREGDADRWQARIHVGGGTLHGGVELKSAANGA
                     MHLSGRLAPRDIELASALATFNRKSPVSGKGSGQTVLSADGKSVGELVRSLHTRTQFS
                     VDAATILRLDLDKAVSTLGKERDGQTALQELTGQLDTQNTEEGMRATYTGLKARSGQY
                     SATGEVTIYRRQLEASGALHLEDGGVGIPFTVSGPVAKPRLSVPPGAFA"
     gene            1498..1851
                     /locus_tag="AC158Q_01275"
     CDS             1498..1851
                     /locus_tag="AC158Q_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIR3338429.1"
                     /translation="MKLVRFIGSAKNDLSAFPRSARLRAGHELFLLQVGRDPDDWKPM
                     SSVGPGACEIRIRNPAGAYRVIYVARFEDAVYVLHAFQKKSPKTSRSDLKLAQLRYRD
                     ARDLAKGIQSGQKSD"
     gene            1832..2116
                     /locus_tag="AC158Q_01280"
     CDS             1832..2116
                     /locus_tag="AC158Q_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3338430.1"
                     /translation="MARKVTESSGNIFLDMGFPPHEAEVMLLRAKLAEALRVWMEREK
                     LTQAEAAKRLGITQPRVSEIVRGKVDLLSLDYLVGLCAKARIPVGLRLAA"
     gene            complement(2185..3717)
                     /locus_tag="AC158Q_01285"
     CDS             complement(2185..3717)
                     /locus_tag="AC158Q_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HIR3338431.1"
                     /translation="MNHDLPDMTASPVLDPALAPGYAELTRVHSRLYRYSHVASIVGW
                     DRNAMMPPKGSEARAAAEAELQALIHRTRTDPRLAEWLRAAEAEPLDDIARANLREIR
                     RDWRDANALPESLVEAKTLAGARCEHAWRSQRPANDWRGFLGNFREVVRLAREEARLL
                     AEDTGLPRYDALMDRFEPGVRAADIDRLFGDVREWLPGLVRRAQAKQGGEKVIVPQGP
                     FPVAAQRALNTEVMTLLGFDFAAGRLDESAHPFSGGVPEDVRLTTRYHEGDFAGSLLG
                     TIHETGHARYEQNLPRAWLGQPVGMARSYGVHESQSLSFEMQLARGRAFAGLLAPLLT
                     RHFGAQPAFEPDNLHRLFTRVKPGLIRVDADEVTYPAHVILRYEIERALIEGEIEAAD
                     IPALWDEKMQALLGLGTRGNDRDGCLQDVHWAEGAFGYFPSYTLGAMYAAQWFAAMRR
                     AAPDLDARIAAGDLAPVFEWLRANIWSQACLWETPELVRRASGEALAPRHFRAPLEAR
                     YL"
     gene            complement(3842..>4532)
                     /locus_tag="AC158Q_01290"
     CDS             complement(3842..>4532)
                     /locus_tag="AC158Q_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338432.1"
                     /translation="LAERSYHQSMMMTDGSLSAFTSRGIPLVLLILTALILLTPAVKL
                     LYRRLAARREGAAAHATSRAPVRAANRLHFEPLIVPVLVLAAAIGMLAWCYQYDGRTR
                     MVPALTAWTLIGFGVLDLVAATGTRIGDYIKAFFTGSLAAPEDDEGKPQPMARILRAC
                     AWVIGYLLALALAGFHLVTPLYVFFYVLRQGRLSVRRALIAAIAAEGFIAFTFEWAME
                     FNLYRGVLFGG"
BASE COUNT          736 a         1529 c         1524 g          743 t
ORIGIN      
        1 ctcgcaaagg cgcccggcgg caccgagagc ctgggcttcg cgaccggacc gctcacggta
       61 aacgggatcc caaccccgcc gtcctcgaga tgcagcgcgc cgctcgcttc gagctggcgg
      121 cggtagatcg tgacctcgcc ggtggcggag tactggcccg agcgcgcctt gagaccggtg
      181 taggtcgcgc gcatgccctc ctccgtgttc tgcgtgtcca gctgacccgt cagctcctgc
      241 aacgcggtct ggccgtcgcg ttccttgccg agagtgctga cggccttgtc caggtcgagg
      301 cgcaggatgg ttgccgcgtc gacgctgaac tgcgtgcgcg tgtgcagcga gcgcaccagc
      361 tcgcccaccg atttgccgtc ggccgacaac accgtctggc ccgagccctt gccgcttacg
      421 ggcgacttcc ggttgaaagt cgccaacgcg ctcgccagct cgatgtcgcg cggggccagc
      481 cggccgctga ggtgcatggc gccgtttgcg gcggacttca attcgacgcc gccgtgcagc
      541 gtgccgccgc ccacatgaat gcgcgcctgc cagcggtcgg catcgccctc acgcttcgcc
      601 gtcagcgtga acggggggct cgcgccgggg cggcgcaact cggcgcggcg gggacgccag
      661 tccgcgtcga aggcgacctc gccgtcatag accacgggaa tgccgctgta ggaaatccac
      721 gtcacgttgg caaatacgaa gtggtccagc gacaggctgc catccgcgcc gggcttgccc
      781 gcgaccggtt ttccgcgaaa ggcgcgcacg gaatcgcgcg gaaagacggc gtcccggatg
      841 gcgatgcgcc ggagcgcgag cttgcggtcc agcagggcgc cggccccggg gtgcgcggtg
      901 agctgcccga tcaccacggg ttgcgcctgc tgcgtgcgaa aattcctgat cactacggcg
      961 ggccggggca gcagcgtcca gtgcgcggag ccgatggtga ccttcacgcc cagccgttcc
     1021 tcggcttcgg cgctcaggcg ggcggcgagt tcttcgtcgg aaggcagcca ccgcgccacg
     1081 gcaagatacg ccgcgcccac cacggcggcc gcaatcccca ggccgagtga ccacttttgc
     1141 ccgcgcttca tggataaata ctcttatgac ccgcacgccg gcgttgattg caattcggcg
     1201 ccatcatgca cataaggaac agaccacaaa ggagcgccgg agtgccgagc tgccgcaatg
     1261 ccgtttagtg tggcccgccc cctggaaatg cgctgtacgc cagcgcacag aacgggctgc
     1321 gacccgtcac ccgccttccc ttgaacggcg tgatacgtga atcgggattc gggattcggc
     1381 ccttttgcct tctgccttcc gcctcggact gcctccttcg tcaacggcca tcaggccaat
     1441 tccgttaacg ggaacaggca atgcttgaca ataatacgaa cattcgtata atcccgcatg
     1501 aagctcgtgc gtttcatcgg cagcgcgaag aacgaccttt ccgcattccc gagatcggcg
     1561 cgattgcgag cgggacacga attgttcctg ctacaggtcg ggcgcgaccc cgacgactgg
     1621 aagccaatgt cttcggtagg gccgggcgcc tgcgagatca ggattcgaaa cccggcagga
     1681 gcgtaccggg tgatttacgt cgccagattc gaagacgccg tatatgtcct gcatgcgttt
     1741 cagaaaaaat ccccgaagac gtcacgttcc gatttgaaat tggcgcaact gcggtaccgc
     1801 gatgcgcgcg atctcgctaa aggaattcag agtggccaga aaagtgactg aatccagcgg
     1861 caatatcttt ctggatatgg gcttcccgcc tcacgaggcc gaggtgatgc tgctgcgcgc
     1921 gaagctcgca gaggctctgc gcgtctggat ggaacgtgag aagctgacac aggccgaagc
     1981 tgcaaagcgc ctgggcatca ctcagcctcg cgtatcggaa atcgtgcgcg gcaaggtgga
     2041 tctgctttcc ctggactacc tggtgggact gtgcgccaag gcgcgcatcc cggttggcct
     2101 gcgccttgcc gcctgaagca gggcgcaagc cgaaagtaga tcccgccagg cagaaccctt
     2161 ttgccttgtg ccttgtgcct tcggctaaag gtatcgcgcc tccaggggcg cgcgaaagtg
     2221 ccgcggggcc agcgcttcgc cgctggcgcg gcgcaccagc tccggcgttt cccagaggca
     2281 cgcctgggac cagatgttgg cgcgcagcca ctcgaacacc ggcgcgaggt cgcccgccgc
     2341 gatgcgcgcg tcgaggtccg gcgccgcgcg ccgcatggcg gcgaaccact gcgccgcgta
     2401 catcgcgccc agcgtgtagc tggggaaata gccgaacgcg ccttccgccc agtgcacgtc
     2461 ctgcaggcag ccgtcccggt cgttgccgcg cgtgccgagc cccagcaggg cctgcatctt
     2521 ttcgtcccac agcgcgggga tgtcggcggc ctcgatttcg ccctcgatca gcgcgcgctc
     2581 gatctcgtag cgcaggatca cgtgggccgg gtaggtcacc tcgtcggcat ccacgcggat
     2641 caggcccggc ttcacgcgcg tgaacaggcg gtggagattg tccggctcga acgccggctg
     2701 agcgccgaaa tgccgggtga gcagcggcgc gagcaggccg gcgaacgcgc ggccgcgcgc
     2761 gagctgcatt tcgaacgaca ggctctggct ctcgtgcacg ccgtacgagc gcgccatgcc
     2821 gaccggctgg cccagccagg cgcgcggcag gttctgctcg tagcgcgcgt gcccggtctc
     2881 gtggatggtg cccagcaggc tgcccgcgaa atcgccctcg tgatagcgcg tggtgaggcg
     2941 cacgtcttcc ggcacgccgc cgctgaacgg atgcgcgctc tcgtcgagcc gtccggccgc
     3001 gaaatcgaaa ccgagcagcg tcatcacctc ggtgttgagc gcgcgctggg ccgccaccgg
     3061 aaaaggcccc tgcgggacga tcactttttc gccgccctgc ttcgcctgcg cgcgcctgac
     3121 caggcccggc agccattccc tgacgtcgcc gaagaggcgg tcgatgtcgg ccgcgcgcac
     3181 gcccggctcg aagcggtcca tcaacgcgtc gtagcgcggg agccccgtgt cctcggcgag
     3241 caggcgcgct tcctcgcgcg cgagccgcac cacttcgcgg aaattcccga gaaacccgcg
     3301 ccagtcgttg gccgggcgct ggctgcgcca ggcgtgctcg cagcgggcgc ccgccagcgt
     3361 cttcgcctcg accagcgatt ccggcagggc gttggcgtcg cgccagtcgc ggcggatctc
     3421 gcgaaggttg gcgcgcgcga tatcgtcgag cggctccgcc tccgccgcgc gcagccattc
     3481 cgcgagccgc ggatcggtgc gcgtgcggtg tatcaacgct tgcagctccg cctcggccgc
     3541 cgcgcgcgcc tcgctgccct tcggcggcat catcgcgttg cggtcccagc cgacgatgga
     3601 cgcgacatgg ctgtagcggt acaggcggga atggacgcgg gtcagctcgg cgtagccggg
     3661 cgccagagcg gggtcgagca cgggcgaagc cgtcatgtcg ggaagatcgt gattcatgga
     3721 cggcgtgatt cgtgaatcgt gattggtgat tggtgattgg tgattggtga ttggtgattc
     3781 gacacttttg ccttgtgcct cgtgccttgt gacttccgcc tttcgccttt cgccttcagt
     3841 ctcatccccc gaacagcacg ccgcggtaga gattgaactc catcgcccac tcgaaggtga
     3901 acgcgatgaa gccctccgcc gcgatcgcgg cgatcagggc ccgcctgacg gacagcctgc
     3961 cctgccggag aacgtagaag aatacgtaaa gcggcgtcac caggtggaac ccggcgagcg
     4021 cgagggccag gaggtagccg atcacccagg cgcaggccct caggatccgg gccatcggct
     4081 ggggcttgcc ttcgtcgtcc tccggcgcgg ccagcgatcc ggtgaagaac gccttgatgt
     4141 agtcgccgat ccgcgtgccg gtcgccgcca cgagatccag cacgccgaac ccgatcagcg
     4201 tccaggccgt cagcgccggc accattcggg tgcgcccgtc gtactgatag caccacgcga
     4261 gcatgccgat ggcggcggcg aggaccagca ccggcacgat gagcggctcg aaatgcagcc
     4321 tgttcgccgc tctcaccggc gcccgtgagg tcgcatgcgc ggctgcgccc tcccggcggg
     4381 cggccagccg gcggtagagc agcttcacgg cgggcgtgag aaggatgagc gccgtaagga
     4441 tcaggagcac gagcgggatg ccgcggctcg tgaatgcgga gaggctgccg tccgtcatca
     4501 tcatggactg gtgatagctg cgctcggcga gc
//
LOCUS       DBNGSG010000057        10609 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_697015, whole genome shotgun sequence.
ACCESSION   DBNGSG010000057 DBNGSG010000000
VERSION     DBNGSG010000057.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 10609)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10609)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10609
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_697015"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="AC158Q_01295"
     CDS             complement(<1..319)
                     /locus_tag="AC158Q_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HIR3338433.1"
                     /translation="MTILHGMLGLLLASASLVVHAQANSIEAINVSPATGGKVVVRVT
                     LKQAPASPPAGFTVNNPPRIAFDFPNTTSALGRSTQDVGEGDLRSINVVQAGDRTRLV
                     LNLS"
     gene            complement(359..877)
                     /locus_tag="AC158Q_01300"
     CDS             complement(359..877)
                     /locus_tag="AC158Q_01300"
                     /inference="COORDINATES: protein motif:HMM:NF016254.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HIR3338434.1"
                     /translation="MKRRYPLVIAVACFALISCSSEQHSDLRQFVKDSDSMPRGRIPP
                     LPEVKPYEPFEYAAFDLIDPFVPRKIEAPKLANVPGALQPPKDHRKGPLEAFPLENLK
                     MVGTLQQQKNFYALIKTPDNNLYRVKPGDFVGQNFGRIIDINESAVKLKELIQDSGSE
                     WKEEERTLLLQE"
     gene            complement(874..1512)
                     /locus_tag="AC158Q_01305"
     CDS             complement(874..1512)
                     /locus_tag="AC158Q_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus inner membrane component PilO"
                     /protein_id="HIR3338435.1"
                     /translation="MAMTVNELQRYLDPKRIGSAPILVKLGVLLVVLVVIIGASWYFD
                     WDDQINRINSEAKKEEGLRKTFLTKKSEAINLPLYQKQLEDIEKQFGALLKQLPGKSE
                     MDALLTDINQAGLGRGLQFELFKPAASETRRDFYAELPITIKISGNYHDIGAFASDIS
                     KLSRIVTLNDIGLTAVKGGVLSLDATAKTFRYLDEDEVAQQRKAAAPKGRKK"
     gene            complement(1517..2098)
                     /locus_tag="AC158Q_01310"
     CDS             complement(1517..2098)
                     /locus_tag="AC158Q_01310"
                     /inference="COORDINATES: protein motif:HMM:NF016992.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HIR3338436.1"
                     /translation="MIRVNLLPHREIRRKAQQQQFFIMLGTVVAIGAGIWFGVHFYLE
                     DQFDNQVSRNKYLQEEIVKLDKQIAEIQKLKDQTAALLARKRVVETLQGTRSEVVHLL
                     DQLVRQLPDGVYLKSIKQAGTRVTIAGLTQSQARVSTLMRNLEASPHLENPGLIEIKG
                     AQQGSLRINEFLMNINITRARPESDTPKPAAKR"
     gene            complement(2095..3195)
                     /locus_tag="AC158Q_01315"
     CDS             complement(2095..3195)
                     /locus_tag="AC158Q_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HIR3338437.1"
                     /translation="MLKGKFNLDALEGLFKAKMPPLIGADISSSAIKMVEIESAGKGM
                     YRIERYAIEPLPKDSVMEGNINNLDAVSDAVKRCHKRLGSNIKNIALALPNAAVISKK
                     ILVPAGQTEDDLALQVETEANQYIPFALEEVNLDFQVLGPAPNSPDDVEVLIAASRKD
                     KIIDRVAVAEAAGLKATVMDVDVFAAQTAFELIESQFTDKGKDQNIAIVDVGASVMAV
                     NVLRNDQSIYMREQPFGGNQLTQEIQNRFGLSPEEAEAAKRAGGLPENYETDVLQPFM
                     DMLGLEVARALQFFFTSTQHNQINHIVLSGGCAAIPGVDEAVGKRVQVNTIVGNPFIN
                     MQVSSKIRAKTLTQDAPSLMVACGLAMRRFDP"
     gene            3425..5749
                     /locus_tag="AC158Q_01320"
     CDS             3425..5749
                     /locus_tag="AC158Q_01320"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HIR3338438.1"
                     /translation="MNIRWWAFPVALLAAGAVGAALLLAFAAAIVYPTLPSLGALTDY
                     QPKIPLRVYSADGQLIGEFGEERRAVVKMQEVPRPMIRAILAAEDERFYQHGGVDYVG
                     VARAALSNFISGGVRQGASTITMQVARNFFLSKERTLTRKFNEMLLAFKIEANLSKDQ
                     ILELYINQIYLGQRAHGFAAAAQIYFGKSLSELTLAESAMLAGLPKAPSRYNPIANPK
                     RAKLRQQYVLRRMRELQLVSAEEFAAADQQPVVVKKEINEFPVRAEYFAEMARQALYE
                     RYKDDTYTKGLRVYTTLLTSHQKAANASLRLGVLEYDRRHGYRGTEGYVELPAKLNDE
                     TLEDALQEFADSEDLYAAIVVEASPKLVRAYRKGGEWVEIKEDGLKFAARMLGEKANP
                     GQRIRRGAIIRVQKDAKGRWQVAQLPAVEASIVALDPRDGAVRALVGGFDFNRSQYNH
                     AIQALRQPGSSFKPFVYSAALEKGFTAATVINDAPLTFTATQTGSETWEPKNFDSKFE
                     GPMRLRTALVKSKNLVSVRILQAISPQYAQDYITRFGFDPKMHPPYLTMALGAGSVTP
                     MQQAAAYAVFANGGYRIAPYFIERVEDARGNVLMMAKPAAVGAGAERVIDARNAFIMT
                     SIMRDVVRMGTAARAARLGRSDLAGKTGTSNEFVDAWFCGYSSDLVAIAWIGFDTPQT
                     LGRNETGAQAALPIWMGYMGAVLKGASERPLVQPNGVIAMRVHPDTGLRVADAKEGIV
                     DYFYQEFLPPEQETLVGGVMGSDRPREEVRNQLF"
     gene            5820..6359
                     /locus_tag="AC158Q_01325"
     CDS             5820..6359
                     /locus_tag="AC158Q_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338439.1"
                     /translation="MAEDGIEDYALAKRKAARQAGTPDTRELPTNEEIDAALRTYQQI
                     YHRDEHRDRLRTLRAIALRAMRELAQFNPYLTGSVLSGNAGKYADINLHLYTDSAKAV
                     EFYLIERKIPYRTAQSRLYCGAEPFTAPVYTVDDDGTEIELTVLTPRELRGALRTSLE
                     GKGIERAKLQAVEVLLAET"
     gene            complement(6360..7367)
                     /gene="hemB"
                     /locus_tag="AC158Q_01330"
     CDS             complement(6360..7367)
                     /gene="hemB"
                     /locus_tag="AC158Q_01330"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011402567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HIR3338440.1"
                     /translation="MRIHGGFPQRRMRRMRRDEFSRRLQREHRLTTDDLIYPVFVIEG
                     KNRAEPVSSMPGVRRFSVDRLLAHAEQCVELGIPALAIFPMIAAELKTPDGREAANAK
                     GLVPRAVAALKKRFPELGVITDAALDPYTTHGQDGVVDRQGYVMNDETITVLEKQALV
                     CAAAGVDVVAPSDMMDGRVGAIRAALDKQKFIHTRILAYSAKYASGFYGPFRDAVGSA
                     KSLGRGNKLTYQMDCANSDEALWEAGLDLDEGADMLMIKPGLPYLDIVRRVKDELRAP
                     TFVYHVSGEYSMLKAACARGWLDERACVMEVLTAFKRAGADGVLTYYAFDVAGWLKAG
                     G"
     gene            7717..8352
                     /locus_tag="AC158Q_01335"
     CDS             7717..8352
                     /locus_tag="AC158Q_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3338441.1"
                     /translation="MNYRWPVMLLAGTLFTPAAMAQGPAKADLPRAQQIVTQVCAACH
                     AADGNSTLAVNPVLAGQHAEYTQKQLLNFKSQGGKPAERPNAVMGGMVANLSTDDMRN
                     LAAYFENQKPKPRAARDRELVKLGQTIYRGGILSKNVAACTACHGPNGMGVPAQYPRI
                     AGQFSEYVAVQLKAFRSGERANDPNRAMRLVAEKLSDKEIEAVSEYISGLR"
     gene            complement(8420..9703)
                     /gene="hemL"
                     /locus_tag="AC158Q_01340"
     CDS             complement(8420..9703)
                     /gene="hemL"
                     /locus_tag="AC158Q_01340"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HIR3338442.1"
                     /translation="MKRSQKLFQRAQLVIPAGVNSPVRAFRSVGGTPPFIQRGKGALV
                     WDADGRAYIDYVGSWGPLVVGHAHPDVVKAVQHAAERGLSFGAPTEQEVEMAELLCRL
                     VPSIEQVRLVSSGTEATMSAIRLARGFTGRSKIVKFEGCYHGHADSLLVKAGSGALTF
                     GQPSSAGVPEAAAADTLVLDYNSDVALEHAFERDGAGIAAVIVEPVAGNMNLVAPRPG
                     FLESLRRLCTRYGTVLIFDEVMTGFRVALGGAQALYGVEPDLTTLGKVIGGGLPVGAF
                     GGRREIMQAIAPLGPVYQAGTLSGNPVAVAAGLATLKLIQSPDFHDRLAANARALCEG
                     LVDCAQKYKIAFSAASVGGMFGVFFRQTAPSSYAEVMQCDKEMFNRFFHAMLVEGIYL
                     APSAYEAGFVSSAHGAAEIERTLTAAEKVFKEMSA"
     gene            complement(9707..10333)
                     /gene="thiE"
                     /locus_tag="AC158Q_01345"
     CDS             complement(9707..10333)
                     /gene="thiE"
                     /locus_tag="AC158Q_01345"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HIR3338443.1"
                     /translation="MKRGISGLYAITPDSSDTTDLLAKVEAALAGGARLLQYRNKTAG
                     PALRLIQARALLALCREYQVPLIINDHLDLALALDAHGLHLGSEDGLLAEARASLAAG
                     KLLGASCYRSIETALDAQRVGADYVAFGGFFPSSVKPSATRTPLALLHEAKQRLSVPV
                     VAIGGITPENARPLVAAGADGVAVISALFGAPDVRAAAQRFDALFPEA"
     gene            complement(10337..>10609)
                     /locus_tag="AC158Q_01350"
     CDS             complement(10337..>10609)
                     /locus_tag="AC158Q_01350"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:NF020132.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HIR3338444.1"
                     /translation="VNTLYGQNGVVRSDSWRRLPGTYHGSGCTLASAIAATIANGLEV
                     SDAVKDAQEYTWQTLKAAFRPGMGQHIPDRLFWARDEEPSGGGREE"
BASE COUNT         2017 a         3219 c         3261 g         2112 t
ORIGIN      
        1 gcgacaggtt gagcacgaga cgggtacggt cgccggcctg aaccacgttg atgctgcgca
       61 agtcgccctc gcccacatcc tgggtgctgc ggccgagcgc gctcgtggtg ttggggaagt
      121 cgaacgcaat gcgcggcggg ttgttcaccg tgaagccggc gggggggctc gcgggcgcct
      181 gtttcagcgt caccctgacg accactttgc ccccggtcgc gggagagaca ttgatggcct
      241 cgatcgagtt tgcctgggcg tggacgacca gactcgcgct cgcgagcagc agccccaaca
      301 tcccatgcaa aatggtcatg acaaaactgg ttgtcggctt catttccctt gcctcctgtt
      361 attcctgcaa cagcagcgtg cgttcttctt ccttccactc gctgccgctg tcctgaatca
      421 gttctttcag cttgacggcg gattcgttga tatcgatgat gcgaccgaag ttttgcccta
      481 cgaaatcgcc tggtttgacg cggtacaggt tgttgtcagg tgttttgatc agcgcgtaga
      541 aattcttttg ctgctgcaga gtacctacca tcttgaggtt ttccagcggg aacgcctcaa
      601 gcggcccctt gcggtggtcc tttggcggtt gcagcgcgcc ggggacgttg gcgagcttgg
      661 gggcttctat tttccgcggc acaaacggat cgatgagatc gaacgcggcg tattcgaacg
      721 gctcgtacgg cttcacttcc ggcaacggcg gaatgcgacc acgcggcatg ctgtccgaat
      781 cctttacgaa ctgccgcaaa tccgaatgct gctcgctgct gcacgatatg agcgcaaagc
      841 aggccaccgc gataacgaga ggataacgac gcttcatttc ttcctgcctt tcggagccgc
      901 ggcttttctt tgctgcgcga cttcatcctc atcgagataa cggaaggtct tggcagtcgc
      961 gtcgagcgac agcaccccgc ctttaacggc ggtaagcccg atgtcgttca gagtgacgat
     1021 ccgggagagc ttgctgatgt cgctggcgaa ggcgccgatg tcatggtaat tgccgctgat
     1081 cttgattgtg atcggtagct cggcgtagaa atccctgcgc gtttcagagg ccgcaggctt
     1141 gaacagctcg aactgcagac cacgccccag gccggcttgg ttgatatcgg tcagcagcgc
     1201 atccatctcg gatttgcccg gcaattgctt gagcagcgcg ccgaactgtt tctcgatgtc
     1261 ttccagctgc ttctggtaga gcggcagatt gatggcttcg ctcttcttgg tcaggaaagt
     1321 cttgcgcagg ccctcttctt tcttggcctc gctgttgatt cgattgatct ggtcgtccca
     1381 gtcgaagtac cagctcgcgc cgatgattac caccagcacg accagcaaca ctccgagctt
     1441 gaccaggatc ggcgcgctgc cgatgcgctt gggatcgaga tatcgttgta gttcgttgac
     1501 ggtcatcgcc atggcatcac ctcttcgccg ccggcttcgg ggtgtcgctt tcgggccggg
     1561 cgcgcgtaat attgatgttc atgaggaatt cgttgatgcg caggctgccc tgctgcgccc
     1621 ccttgatctc gatcagcccg gggttttcca gatggggcga ggcttccagg ttgcgcatca
     1681 gcgtggacac gcgtgcctgg gactgcgtga ggccggcgat tgtgacccgg gtgccggcct
     1741 gcttgatgct cttcaggtag acgccatcgg gcaattgccg caccagctga tcgagaagat
     1801 ggactacttc cgaacgggtg ccctgcagcg tctctaccac ccgcttgcgt gcgagcagcg
     1861 ccgcggtctg gtccttcagc ttctgaatct ccgcaatctg cttgtccagc ttgacaatct
     1921 cttcctggag atacttgttg cggctcactt ggttgtcgaa ctggtcttcc aggtagaaat
     1981 gcacgccaaa ccagatgccg gcaccgatag ccaccaccgt gccgagcatg atgaagaact
     2041 gctgctgttg tgccttgcgc cgaatctcgc gatgcggcag taggttgacg cgaatcatgg
     2101 gtcgaacctc cgcatggcca gcccacacgc caccatgagc gaaggtgcgt cctgcgtcag
     2161 ggtcttcgcc cgtatcttcg aggaaacctg catgttgata aacgggttgc ctacaatcgt
     2221 attgacctgc acgcgtttac ccacggcctc atctacgccc gggatcgccg cacatccgcc
     2281 cgagagcacg atgtggttga tctgattgtg ctgggtcgag gtgaagaaga actgaagtgc
     2341 ccgggccact tccaggccaa gcatatccat aaacggctgg agcacgtcgg tttcgtaatt
     2401 ctccggcaac ccgccggcgc gcttggcagc ttccgcctct tccgggctca acccgaagcg
     2461 gttctggatc tcctgggtga gctggttgcc gccgaacggc tgttcgcgca tgtaaatcga
     2521 ctgatcgtta cgcagcacgt tgactgccat gacggaggcg cccacgtcga caatcgcgat
     2581 attctggtcc ttgcccttgt cggtaaactg cgattcgatc agttcgaagg cagtctgcgc
     2641 ggcgaatacg tcgacatcca tcaccgtcgc cttcagtccc gccgcctcgg ctacggcgac
     2701 ccggtcaatg atcttgtcct tgcgggaagc agcaatcagc acttcgacgt cgtccgggct
     2761 gttgggggcg ggccccagca cctggaaatc gagattgact tcctcgagag cgaacggaat
     2821 gtactgattt gcttcggttt caacctgcaa cgcaaggtca tcctcggtct gcccggctgg
     2881 caccaggatc ttcttgctga tcacggctgc attgggcagc gcaagcgcga tgttcttgat
     2941 attgctgcct aaccgcttat ggcaccgctt taccgcgtca cttaccgcgt caaggttgtt
     3001 gatgttgcct tccatcaccg agtccttggg cagcggctcg atggcgtagc gctcgatgcg
     3061 atacatgcct ttgccagcgc tctcgatctc caccatcttg atcgccgagg aactgatgtc
     3121 ggccccgatc aacggcggca tcttggcctt gaacaggcct tcgagggcat cgaggttaaa
     3181 ttttcctttg agcataagtt tatggggaga ctatcccaca gtttacatta gatgttagca
     3241 acaactccct tggatgtaaa gcattttgtc ttcttttaaa tttgaaggga accccgtcta
     3301 taatggaggg gcactaaata tagcttactc cgcagcaagt tgctgcgacg gggttcacgc
     3361 atgggctgtt aggtgccctt cttgttgtaa tgtcgcaaca aacatatact aaggcactga
     3421 tttaatgaac attcgctggt gggccttccc tgtagccctg ttggccgccg gtgctgtggg
     3481 cgcggctttg ttacttgcgt ttgctgccgc tattgtttat cccacgctgc cttcactggg
     3541 ggcgctcacc gattatcagc cgaagattcc gttgcgcgtc tacagcgccg acggacagtt
     3601 gatcggcgaa tttggcgagg agcgccgtgc cgtggtgaaa atgcaggaag ttcctcgacc
     3661 catgatacgc gccatcctgg cggccgaaga tgaacgtttc taccaacacg gcggggtgga
     3721 ctacgtaggc gtggcaaggg ccgccctgtc caatttcatt tctggtggcg tgcgccaggg
     3781 cgcctccact atcaccatgc aggtcgcccg caatttcttt ctttccaaag agaggacatt
     3841 gacgcgcaag ttcaacgaaa tgctgctcgc tttcaagatc gaggccaatc tcagcaagga
     3901 ccagatcctt gagctctata tcaaccagat ctaccttggc cagcgcgcgc acggctttgc
     3961 cgccgccgcg caaatttatt ttggcaagag cctttccgaa ttgactcttg ccgaatccgc
     4021 gatgcttgca ggactcccca aggccccttc gcgttacaac ccgattgcca atcccaaacg
     4081 agccaagttg cggcagcaat acgtgttgcg gcgaatgcgc gaattgcagc tcgtttcggc
     4141 agaagaattt gctgccgcgg accaacagcc ggttgtggtg aagaaggaaa tcaatgagtt
     4201 cccggttcgc gcggaatatt tcgccgagat ggcgcggcag gcactctacg aacgttacaa
     4261 ggacgacact tacaccaagg gccttcgggt gtacaccacc ttgttgacat cgcaccagaa
     4321 ggcggccaat gcgtcgctgc gcctcggcgt cctggagtat gaccggcgtc acggctaccg
     4381 cggcaccgaa gggtatgtcg aattgccggc aaagctgaac gacgaaacac tggaggatgc
     4441 gctgcaggaa ttcgctgaca gcgaagatct ttacgccgcc atcgtcgttg aggccagtcc
     4501 caagctggtg cgggcttacc gcaagggcgg agagtgggtc gagatcaagg aagacgggct
     4561 caagttcgcg gcccgcatgc tgggcgagaa ggcaaacccc gggcaacgca tacgccgcgg
     4621 cgccatcatc cgcgtgcaga aagatgcgaa agggcgctgg caggttgcac agttgcccgc
     4681 ggttgaggct tcgatcgtcg cgctcgatcc gcgtgacggt gcggtgcggg cattggtcgg
     4741 gggctttgat ttcaaccgca gccagtacaa ccatgccatt caggcgctga gacagccggg
     4801 ctccagcttc aagccctttg tctactcggc ggcgcttgag aaaggcttca ccgctgcaac
     4861 cgtcatcaac gacgcgccac tcacgtttac agcgacgcag accggatcgg aaacttggga
     4921 accgaagaac tttgacagca agttcgaagg gccgatgcgg ctgcgcactg cgctcgtcaa
     4981 gtccaaaaac ctggtttcgg tgcgcatcct gcaggcaatc tccccgcagt acgcgcagga
     5041 ttacatcacg cgctttggat tcgatccaaa aatgcacccg ccgtatctca ctatggcgct
     5101 cggcgcgggc agtgtgacgc cgatgcagca ggccgccgcc tacgcggttt tcgcgaacgg
     5161 cggttaccgc attgcgccgt atttcatcga gcgcgtggaa gacgcgcgcg gcaatgtgct
     5221 gatgatggcg aagccagcgg cggtaggcgc gggggcggag cgcgtcatcg atgcacgcaa
     5281 cgctttcatc atgaccagca tcatgcgcga cgtggtgcgt atgggcactg ccgcgcgggc
     5341 agcaaggctg gggcgcagtg atctcgccgg caagaccggc accagcaacg agttcgtgga
     5401 cgcgtggttt tgcggctaca gttccgattt ggtggcgatt gcctggattg gcttcgacac
     5461 gccgcagacg ctcgggcgca acgaaaccgg cgcacaggcg gccctgccaa tctggatggg
     5521 atacatgggc gcagtattga agggggcatc ggaacggccg ctcgtgcagc ccaacggggt
     5581 gattgccatg cgggtccacc cggacaccgg attgcgcgtt gccgacgcca aggagggaat
     5641 tgtcgactat ttctaccagg aattcctgcc gcccgagcag gagacactgg ttggcggcgt
     5701 gatgggaagc gaccgcccgc gggaagaggt acgcaatcag ttgttctgag ccggccatgc
     5761 ccagggagac aggtcgcaac gaccggcgca gccacatcgc tcaccttgcg gcgcggctga
     5821 tggccgagga cggcatcgag gattatgcgc tcgccaagcg caaagccgcg cgtcaggccg
     5881 gcacaccgga tacccgcgag ctgccaacca atgaggaaat cgacgcggcg ctcaggacct
     5941 atcagcagat ttaccaccgg gatgagcacc gtgatcggtt gcggacgctg cgggcgatcg
     6001 cgctgcgggc gatgcgggaa ctcgcgcagt tcaatccgta cctgaccgga tcggtcttga
     6061 gcggcaatgc cgggaagtat gccgacatca accttcactt gtacaccgac agtgcaaagg
     6121 cggtcgagtt ctacctcatt gagcgcaaaa tcccgtaccg gacggcgcag agcaggctat
     6181 attgcggcgc cgagccgttc accgcgccgg tatacaccgt cgacgatgac ggcactgaaa
     6241 tcgagttgac cgtgctcaca ccacgcgaat tgcgcggagc gctgcgcacc agcctcgagg
     6301 gaaagggcat cgagcgcgcc aaactacagg ctgtggaagt cctgctcgcg gaaacctgat
     6361 cagccgccgg ctttcagcca gccggccaca tcgaacgcat agtaggtaag aacgccgtcg
     6421 gcgcccgcgc gcttgaacgc ggtgagaact tccatcacgc acgcgcgctc gtcgagccag
     6481 ccacgcgcgc aagcggcctt caacatcgag tactcgccgc tcacgtggta gacgaatgtg
     6541 ggcgccctga gctcgtcctt cacgcggcgc acgatatcga ggtaaggcag gccgggcttg
     6601 atcatcaaca tatccgcgcc ttcgtcgaga tcgaggccgg cttcccacaa cgcctcgtcg
     6661 ctgttggcgc agtccatctg gtaagtgagc ttgttgccgc gccccagact ttttgccgag
     6721 cccaccgcgt cgcgaaacgg cccgtagaag ccggaggcgt acttggccga ataggcgagg
     6781 atgcgcgtgt gtatgaattt ctgcttgtcg agcgcggcgc ggattgcgcc aacgcgtccg
     6841 tccatcatgt ccgaaggcgc caccacgtcg acgcccgccg cggcgcagac cagcgcctgc
     6901 ttttccagca cggtgatcgt ttcgtcgttc atcacgtagc cctgacggtc gactacgccg
     6961 tcctggccgt gcgtggtata ggggtcaagc gcggcgtccg tgatcacgcc cagctccggg
     7021 aagcgcttct tcagcgcggc taccgcgcgc ggcaccagtc ctttggcgtt ggcggcctcg
     7081 cggccatcgg gggtcttgag ctcggcggcg atcatgggga agattgccaa tgccgggatt
     7141 ccaagttcta cgcactgctc ggcgtgcgcc agcaggcggt ccacgctaaa gcgtcggaca
     7201 ccgggcatgg acgacaccgg ctcggcgcgg ttcttccctt cgattacgaa taccgggtag
     7261 atgagatcat ccgtagtgag ccggtgctcg cgctgcagcc gccgcgagaa ctcgtcgcgc
     7321 cgcatccggc gcatgcgccg ttgcgggaag ccgccgtgga ttcgcatcgt attaaccttt
     7381 tgaacgaatt tgttattttc gggcggggaa ctatcgatca ttataatcgt cttaatctac
     7441 agacgatgtt gagtcgtctc ccgcttctcc tccctgagcg ggcgccttaa tcctagttaa
     7501 ggcttttgcc cccggtttta ctccccttta accgggggtt ttttatgtac agaccggacg
     7561 atcaggcgcc gcagttcaca tcgccggagc cgcccagcag agcggcgaaa atcatctagg
     7621 ttattgttat cgcgcctgaa actggcgctc ggcgaagcgc ttactataca atacgcgttc
     7681 cgcataaata acaatgacgt tagagaggaa atccgcatga attaccgctg gcccgtcatg
     7741 ctgcttgccg gaacgctgtt caccccggct gcaatggcgc aaggtcccgc caaggcggac
     7801 cttcccaggg cgcagcagat cgtgacgcag gtatgtgcgg cctgtcatgc ggcagacggc
     7861 aacagtacat tggccgtcaa cccggtgctc gccgggcagc atgccgagta cacccaaaag
     7921 cagctcctga acttcaagtc gcagggcggc aagccggccg agcggccgaa tgccgtaatg
     7981 ggcggcatgg tggcgaatct ctcgaccgat gacatgcgca atctggccgc ctatttcgag
     8041 aatcagaagc ccaagccgcg ggcagcgcgc gaccgggaat tggtcaagct cggtcagact
     8101 atctaccgcg gcggcatact gtcgaagaac gtggcggcat gtactgcctg ccatggtccg
     8161 aatggtatgg gcgtgccggc ccagtatccc cggatcgccg ggcaattttc cgagtacgtt
     8221 gcggtacagt tgaaggcgtt tcggtcggga gagcgagcca acgatcccaa tcgtgcaatg
     8281 cgcttggtgg ccgagaagct ctccgacaag gagatcgaag cagtctcgga atatatctcc
     8341 ggcctgcgct gatccgggcg gcgactgaca aggggcgaac ccctttttgg tcgcgatgga
     8401 cagtaaggcc gagacaccat caggcgctca tctccttaaa taccttttct gcggcggtga
     8461 gcgtccgctc aatctcggct gcaccatgcg ccgaagaaac aaagccggcc tcgtatgccg
     8521 agggggcgag gtagatgcct tcgacgagca tcgcgtggaa aaaccggtta aacatctcct
     8581 tgtcgcattg catgacctcg gcgtagctgg agggggccgt ctggcggaaa aacacgccga
     8641 acatgccgcc gacgctcgcg gccgaaaagg cgatcttgta tttctgcgcg caatcgacca
     8701 ggccctcgca cagcgcgcgc gcattggccg ccagcctgtc gtggaagtcc ggcgactgga
     8761 tgagcttcaa tgtggcgagc cccgctgcga ccgcgaccgg gttgccggat agcgtgccgg
     8821 cctgatagac cgggccgagg ggagcgatgg cctgcataat ttcgcgtcgt ccgccgaagg
     8881 cgccgaccgg caagccgccg ccaatcactt tgccaagcgt ggtgagatcc ggttccacgc
     8941 catagagcgc ctgagcgccg ccgagtgcca cacggaaacc ggtcatcact tcgtcaaaga
     9001 tcagcactgt gccgtaacgg gtgcagagcc tgcgcagcga ttctaggaaa cctgggcgcg
     9061 gcgccaccag gttcatgtta ccggcaaccg gttccacgat cactgccgcg atgcccgcgc
     9121 cgtcgcgttc gaaggcgtgc tccagcgcaa cgtcactgtt gtaatcgagc accagggtat
     9181 cggccgcggc agcctccggc acgcccgccg aggaaggctg gccgaaagtg agcgcccccg
     9241 atccggcctt cacgagaagc gagtcggcat ggccgtgata acacccttcg aacttgacga
     9301 tcttgctgcg gccggtaaaa ccacgagcaa ggcgtatcgc gctcatcgtc gcctccgtgc
     9361 ctgaactgac caggcgcacc tgctcgatcg aaggcaccag ccggcacagg agttccgcca
     9421 tctcgacttc ctgttcggta ggcgcgccga atgagaggcc ccgctcggca gcgtgctgga
     9481 ccgctttcac cacgtcggga tgcgcgtgcc ccacgacgag cggcccccat gaaccgacgt
     9541 agtcgatgta ggcgcggccg tcggcgtccc ataccagcgc gcccttgccg cgctggatga
     9601 agggcggcgt gccgccgacc gagcggaatg cccgcaccgg cgagttgacg ccggcaggta
     9661 tgacgagttg cgcgcgctgg aacagtttct ggctgcgttt catttctcat gcctcgggaa
     9721 aaagggcgtc gaaacgttgc gccgcagcgc ggacatcggg tgcaccgaac agcgccgaaa
     9781 tcaccgccac gccatcggcg ccggcggcga ccagcggtcg tgcgttttcg ggcgtgatgc
     9841 cgccgatcgc aaccaccggc accgaaagcc gttgcttggc ctcgtgcagc agggcgagcg
     9901 gggtgcgcgt cgcgctgggc ttgacggacg aggggaagaa tccgccgaat gccacgtaat
     9961 ccgcgccgac gcgctgggcg tccagtgcag tttcgatact gcgatagcag gacgcgccga
    10021 gtagcttccc ggcggcgagg ctggcacgcg cttccgcgag caggccgtcc tcggagccca
    10081 ggtgcaggcc atgcgcatcg agcgcgagcg cgagatcgag atgatcgttg atgatgagcg
    10141 ggacctggta ttcccggcac agcgcgagca gcgctcgggc ctgtatcagc cgtaacgccg
    10201 ggcccgcggt cttgttgcgg tattgcagca ggcgcgcacc tccggccagc gccgcttcaa
    10261 ccttggcgag cagatcggtg gtgtcgctgc tatcaggcgt gatggcgtaa aggccggaaa
    10321 tcccgcgctt cacgcgctac tcctcgcgac cgccgcctga cggttcctca tcacgcgccc
    10381 agaacaggcg gtccggaatg tgctggccca tgccgggacg gaacgccgct ttaagcgttt
    10441 gccaggtgta ttcctgggca tccttcaccg cgtcgctgac ctcgagccca ttggcgatgg
    10501 tggcggcgat ggcggacgcg agcgtgcagc ccgagccatg gtaggtgccg ggcaggcgcc
    10561 gccaggagtc gctgcgcacc acgccgttct ggccgtaaag ggtgttgac
//
LOCUS       DBNGSG010000058         4798 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2394902, whole genome shotgun sequence.
ACCESSION   DBNGSG010000058 DBNGSG010000000
VERSION     DBNGSG010000058.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4798)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2394902"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /gene="selD"
                     /locus_tag="AC158Q_01355"
     CDS             complement(<1..452)
                     /gene="selD"
                     /locus_tag="AC158Q_01355"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(393..395),aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HIR3338445.1"
                     /translation="MNTPSDTRIRLTQLSHGGGUGCKLTPALLSEVLAGSPAGRVFPD
                     LLVGNESSDDAAVYRINDRQALVATTDFFTPIVDDPYDFGRIAATNAISDVYAMGAKP
                     FLALALLGFPLGKLPVEAVQRIVAGGHDACTKAGVPIGGGHSIDSPEP"
     gene            476..1609
                     /gene="mnmH"
                     /locus_tag="AC158Q_01360"
     CDS             476..1609
                     /gene="mnmH"
                     /locus_tag="AC158Q_01360"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HIR3338446.1"
                     /translation="MHATHNPGRGEKQRVATGIAVRGPALVTVAQLSEFDEIIDARSE
                     GEFAEDHVPGAINCPVLDNDERARVGTIYKQRSSFEAKKIGAALVSANIARHLQARFL
                     DRPREWRPLVYCWRGGSRSDAFAHVLHQVGWRAGRIDGGYRAYRRAVVDQLVELPGRF
                     RWRVICGLTGAGKSRLLRALQEAGGQTLDLEALAAHRGSVLGNLPGEPQPSQKRFESR
                     VWKALTGFDASRPVYVEAESRKIGALRVPEALIERMWAAECVVLESPVEARVELLKQE
                     YAHFFDQVADLNAKLECLTPLHGHVVIEKWKTLARAGNWSELTADLLVRHYDPAYTRA
                     IVKHYPALDRAPRLSLERADEGEFSALARRFLESESDRDRVKA"
     gene            1665..2543
                     /locus_tag="AC158Q_01365"
     CDS             1665..2543
                     /locus_tag="AC158Q_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR3338447.1"
                     /translation="MKLALRIEVATLRGTREAAPRLAALLKEYGAGATFLFSLGPDRT
                     GRALGSLPRVPKWSCYGPAALLSGTLLPGPDIGSRCADEMRAVRSQGFEAGILAYDRA
                     DWVRHAARAGETWTQTAMQLARDRYEDVFQAHALTHGAPGWQMNRFAYRYTQRLGFRH
                     CSDTRGTGPFIPVLNAEIAACPQLPTTLPTLDELLAGGLNPEEAVRRLLELARKPPTG
                     GHVHTLRAEVEGTVFEPVLRALLEGWRGLGIELVSLREYASALDLAQLPRRVIVEQPV
                     PGGEGAVSAQGKEFLT"
     gene            2812..3273
                     /locus_tag="AC158Q_01370"
     CDS             2812..3273
                     /locus_tag="AC158Q_01370"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR3338448.1"
                     /translation="MLDQPAPDFELPATGGKIVRLARLKGSPLVLYFYPKDNTPGCTT
                     EGQNFRDLHPEFQKLGCAIFGVSRDSLKSHENFKAKMAFPFDLLSDTEETACKLYDVM
                     KMKNMYGKKVRGIERSTFAIDAKGTVRGMWRGVKVPGHVQEVLEFVKTLKV"
     gene            3370..>4798
                     /locus_tag="AC158Q_01375"
     CDS             3370..>4798
                     /locus_tag="AC158Q_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008164313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIR3338449.1"
                     /translation="MNQRKAAAAPTQSSAHSSVVARLPAAIKLFVLDTNVLMHDPTSL
                     FRFEEHDLYVPMATLEELDNNKKGMSEVSRNARQASRYLEEMLAHWEDGISEGIALGA
                     HGDKAATGRLFLQTEAITGELPARLASGKADNQILAVVKYLQELHPKRQVILVSKDIN
                     MRIKARALGLAAEDYFNDKVLEDTDLLYTGVRSLPEDFWERHGKEMESWQQHGHTYYR
                     VHGPLVSQLLVNEFVYLEADRPFHAQVKEVSGKTAILVTLRDHAGQRHNVWGITARNR
                     EQNFALNMLMNPQIDFVTLLGQAGTGKTLLTLAAGLMQTLEQKTYTEIIMTRVTVPVG
                     EDIGFLPGTEEEKMNPWMGALEDNLDVLNKPSDEAGEWGRAATRDLIRSRIKVKSLNF
                     MRGRTFINKYLIIDEAQNLTPKQMKTLITRAGPGTKVVCLGNISQIDTPYLTEGSSGI
                     TYVVDRMKGWAHSAHVTLARGERSRL"
BASE COUNT          934 a         1596 c         1495 g          773 t
ORIGIN      
        1 atcggctccg gcgagtcgat cgaatgcccg ccgccgatcg gcacgcccgc cttcgtgcac
       61 gcatcgtgcc cgccggccac gatgcgctgc accgcctcca cgggcagctt gccgagcggg
      121 aagccgagca gcgccagcgc gagaaacggt ttcgcgccca tggcatagac atcggagatc
      181 gcgttggtcg ccgcgatgcg cccgaaatcg tacggatcgt ccacgatggg cgtgaaaaaa
      241 tcagtcgtgg cgaccagcgc ctggcgatca ttgatgcgat agaccgcagc gtcgtcgctc
      301 gactcgttgc caaccagcag gtccggaaaa acccggccgg cgggcgagcc cgcaagcacc
      361 tcgctcagca acgctggcgt gagcttgcag cctcagccgc cgccgtggga gagctgggtg
      421 agccggatgc gcgtgtctga tggtgtgttc atgatttacc gtaaaatagc cgccgatgca
      481 cgcaacccac aacccgggcc ggggcgaaaa acagcgcgtt gcgacaggga ttgccgtgcg
      541 tggacccgcg cttgttaccg tagcacaact gtccgagttt gacgagatta tcgacgcccg
      601 ctccgagggc gagttcgccg aagatcacgt acccggcgcg atcaattgtc ccgtgctcga
      661 caacgacgag cgcgcgcgcg tcggcacgat ctacaagcag cgctcttcct tcgaagccaa
      721 gaaaatcggc gcggcgctcg tcagcgccaa catcgcgcgc catcttcagg cacgcttcct
      781 cgatcgcccg cgcgagtggc gcccgctggt ctactgctgg cgcggaggca gccgcagcga
      841 cgccttcgcg cacgtgctgc accaggtcgg atggcgcgcg ggccgcatcg acggcggcta
      901 ccgtgcctac cgccgcgccg tcgtggacca gctcgtcgaa ttgcccgggc ggtttcgctg
      961 gcgggtcatt tgcgggttga ccggagcggg aaagagccgc ctgttacggg cgctgcagga
     1021 agcgggcggg cagacactcg atctcgaggc gctggcggcc caccgcggct cggtgctggg
     1081 caatctcccc ggcgagccgc agccgtcgca aaaacggttc gaaagccggg tctggaaagc
     1141 gctcaccggc ttcgacgcat cgcggccggt ctacgtcgag gcggagagca ggaagatcgg
     1201 cgcgctgcgc gtgccggaag ccctgatcga gcggatgtgg gcggccgaat gcgtggttct
     1261 ggaatccccg gtcgaagccc gcgtcgagct gctcaagcag gagtacgccc actttttcga
     1321 ccaggtcgcg gacctgaacg cgaaactgga gtgcctgacg ccgctgcacg gccacgtggt
     1381 catcgagaaa tggaaaacgc ttgcccgcgc gggaaactgg agcgagctca ccgccgacct
     1441 gctcgtccgt cactacgacc ccgcttacac ccgcgccatc gtgaagcact atcccgcgct
     1501 cgaccgcgcg ccccggctga gcctcgagcg cgcggatgaa ggcgagtttt cggcgctcgc
     1561 ccggcgcttc ctcgaatcgg aatctgaccg tgatcgggtg aaagcatgac tcgcgtgaga
     1621 ctcttcgcct ttcactcatc actcatcatt catcattcat cactgtgaaa ttggccctgc
     1681 gcatcgaggt ggcaacgctg cgcggtacgc gcgaggccgc gccgcggctc gcggctctcc
     1741 tcaaggaata cggtgcgggc gccacgtttc tgttcagtct cggcccggac cgcaccgggc
     1801 gcgcgctggg cagcctgccg cgcgtgccga aatggagttg ttacggaccc gcggcgctgc
     1861 tgtcgggaac gctgctgccg ggacccgaca tcggctcgcg ctgcgcggac gaaatgcgcg
     1921 ccgtgcgctc gcagggattt gaagccggca tcctggccta cgaccgcgcg gactgggtgc
     1981 gccacgccgc gcgggcgggc gagacatgga cccagacggc gatgcaactg gcccgcgatc
     2041 gctacgagga cgtgttccag gcgcacgcgc ttacccacgg cgcccccgga tggcagatga
     2101 accgattcgc ctatcgttac acccagcggc tcggtttccg gcactgctcc gatacccgcg
     2161 gcaccgggcc tttcattccc gtgctgaacg ctgaaatcgc tgcctgcccg caactgccga
     2221 ccacgctgcc cacgctcgac gagttgctcg ccggcggcct caatccggag gaagcggtgc
     2281 ggcgcctgct ggaactcgcc cgcaagccgc cgaccggcgg tcacgtgcac acgctgcgcg
     2341 ccgaagtcga aggcacggtg ttcgagccgg ttctgcgggc gctgctcgaa ggctggcgcg
     2401 gcctcggcat cgaactcgtt tcgctgcgcg aatacgcttc ggcgctcgat ttggcccaac
     2461 tgccgcgccg ggtcatcgtc gagcaacccg tgccgggcgg cgagggcgcc gtttcggcgc
     2521 agggcaagga attcctgacg taaaaatgga caggattaag gaacctccgc ataagtcatc
     2581 cccgcgcagg cggggatcca gtaacgagtt gtttaccctg gattcccgcc ttagcaggaa
     2641 tgacgaaaaa cgacttttgc agagcatcct taacaagatt tacaggatta ctgcctgaac
     2701 attgtctgtt taatcctgta aatcccgttg attgccctcg ctgcttgcgt gggaagcctg
     2761 attcggtcaa cataactgca ggccatcgcg agacgaggtc cggcccaccg aatgctagac
     2821 cagccagcac ccgatttcga gttgcccgcc accggcggca agattgtccg cctggcccgc
     2881 ctcaaaggct ctcccctcgt cctttacttc taccccaagg acaacacgcc cggctgcacc
     2941 accgagggcc agaatttccg cgatctccac ccggaattcc agaagctggg gtgcgcgatc
     3001 ttcggagtgt cgcgcgacag cctgaagtcg cacgagaatt tcaaggccaa gatggccttc
     3061 cccttcgatc tgctgtccga caccgaagag accgcctgca agctctacga tgtcatgaag
     3121 atgaaaaaca tgtatggcaa gaaggtacgg ggcatcgaga ggagcacgtt cgccatcgac
     3181 gccaagggaa cggtgcgcgg catgtggcgc ggcgtgaaag tccccggcca tgtccaggaa
     3241 gtgctcgaat tcgtcaagac actcaaagtg tgaggtttca agttcccagt ttcaagttac
     3301 atacggcggc aggactctca acttgaaacc tgaaactcga aacttgaaac tcagtagcca
     3361 gggaggttca tgaaccaacg caaggccgca gccgcaccca cgcagtcctc cgcgcactcg
     3421 tcagtcgttg cccgtctacc cgccgccatc aagctcttcg tgctcgacac caacgtgctg
     3481 atgcacgatc ccaccagcct gttccgcttc gaagagcacg acctctacgt cccgatggcc
     3541 accctggagg agctcgacaa caacaagaag ggcatgtcgg aagtgtcgcg caacgcgcgc
     3601 caggcgagcc gctacctcga ggaaatgctc gcccactggg aagacggcat atcggagggc
     3661 atcgcgctcg gggcgcacgg cgacaaggcc gccaccggcc gcctgttcct gcagaccgag
     3721 gccatcaccg gagagctgcc ggcgcggctc gccagcggca aggccgacaa ccagatcctc
     3781 gcggtggtca agtatctgca ggagctgcat cccaagcgcc aggtgatcct ggtgtcgaaa
     3841 gacatcaaca tgcgcatcaa ggcgcgcgcg ctggggctcg cggccgagga ttactttaac
     3901 gacaaggtgc tggaggacac cgacctcctc tacaccgggg tgcgctcgct gcccgaggat
     3961 ttctgggaac gtcatggcaa ggagatggaa tcctggcaac agcacggaca tacctattac
     4021 cgcgtgcacg gcccgctggt ctcgcagctg ctggtgaacg agttcgtcta cctcgaggcg
     4081 gaccgcccgt tccacgccca ggtcaaggag gtgagcggca agaccgccat cctcgtgacg
     4141 ctgcgcgatc acgccggcca gcgccacaac gtctggggca tcacggcgcg caaccgcgag
     4201 cagaatttcg ccctcaacat gctgatgaat ccgcagatcg acttcgtgac gctgctaggc
     4261 caggccggca ccggcaagac gctcctcacg ctcgccgccg ggctgatgca gacgcttgag
     4321 caaaagacct acaccgaaat catcatgacg cgggtcacgg tgcccgtcgg cgaggacatc
     4381 ggcttcctgc ccggcaccga ggaggagaag atgaacccgt ggatgggcgc gctcgaggac
     4441 aacctcgacg tcctcaacaa gccctccgac gaggccggcg aatggggccg cgcagccacc
     4501 cgcgacctga tccgctcgcg catcaaggtg aaatcgctca atttcatgcg cgggcgcacc
     4561 ttcatcaaca agtacctgat catcgacgag gcgcagaatc tgacgccgaa acagatgaag
     4621 acgctgatca cgcgcgccgg gcccggcacc aaggtggtgt gcctcggcaa catctcgcag
     4681 atcgacacgc cctacctcac cgaagggagc tccggcatca cctacgtcgt cgaccgcatg
     4741 aagggatggg cgcacagcgc gcacgtcacg ctggcgcgcg gcgaacgctc gcggctgg
//
LOCUS       DBNGSG010000059         1794 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3583627, whole genome shotgun sequence.
ACCESSION   DBNGSG010000059 DBNGSG010000000
VERSION     DBNGSG010000059.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3583627"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..>1794)
                     /locus_tag="AC158Q_01380"
     CDS             complement(81..>1794)
                     /locus_tag="AC158Q_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HIR3338450.1"
                     /translation="VLAGPTIIAEANATTVIEPQWQAQVTPLNHLVLTRSVARPHRAA
                     VGTQAEPVMLEIFNNLFMAIAEQMGVTLANTAYSVNIKERLDFSCALFDPVGGLIANA
                     PHLPVHLGAMGESVRVVATENAGRIRPGDAYTLNNPYHGGTHLPDLTVITPVFDAAGK
                     DILFYVACRGHHADIGGITPGSMPPDSRHLEEEGVLIDNFKLVSEGRFRESETRALLA
                     GARYPARNPEQNLADLRAMVAANQKGVTEIARAVEHYGLDVVQAYMRHVQDNAEESVR
                     RVIDVLKVGNGDPAVPSRDGEFAYEMDNGAVIRVKITVDRASRGATIDFTGTSGQLDS
                     NFNAPKAVTMSAVIYVFRTLVNDDIPLNAGCLKPIRVIVPEGCMLNPRYPAAVVAGNV
                     ETSQCVTDALYGALGVMGASQGTMNNFTFGNDRYQYYETVCGGSGAGEGFDGADCVHT
                     HMTNTRLTDPEVLEWRYPVLIEEFAVRRGSGGGGRWRGGDGAIRRIKFLEPMTAAILS
                     GHRRLPPYGMAGGKPGQTGHNRVRHSGGTMVELKGCDRVEMGAGDVFIIETPGGGGYG
                     PPGG"
BASE COUNT          264 a          590 c          622 g          318 t
ORIGIN      
        1 tcccttctcc actcgggaga aggggaaggg atgaggggat ggcgggcacc cctcctcagg
       61 caggagggga gttaccgaga tcatcccccg ggcgggccgt agccgccgcc gcccggcgtc
      121 tcgatgatga agacatcgcc cgcgcccatc tcgacgcggt cgcatccttt cagctcgacc
      181 atcgtgccgc ctgaatgccg cacccggttg tgcccggttt ggccgggctt gccgcccgcc
      241 atgccgtagg gcggcaggcg ccggtggccg gagaggatcg cggcggtcat cggctcgagg
      301 aacttgatgc ggcggatggc gccgtcgccg ccgcgccagc gcccgccgcc gccgctgccg
      361 cgccgcaccg cgaactcctc gatcagcacc gggtagcgcc attccagcac ctccgggtcg
      421 gtcaaccggg tgttggtcat gtgagtgtgg acacagtccg cgccgtcgaa accctcgccg
      481 gcgcccgaac ccccgcagac cgtctcgtag tactggtagc ggtcgttgcc gaaggtgaag
      541 ttgttcatcg tgccttggga ggcgcccatg acgccgagcg ccccgtagag cgcgtcggtc
      601 acgcactggg aggtctcgac attgcccgcc accaccgccg ccgggtagcg gggattcagc
      661 atgcagccct cggggacgat gacccggatc ggcttcaggc acccggcgtt gagcgggatg
      721 tcgtcgttca ccagcgtgcg gaagacgtag atcacggccg acattgtgac cgccttcggc
      781 gcgttgaaat tgctgtcgag ctggccggag gtgccggtga aatcgatcgt ggcgccgcgg
      841 cttgcccggt ccacggtgat ttttacgcgg atcaccgcgc cgttatccat ctcgtaggcg
      901 aattccccat cccgggatgg aacggcgggg tccccgttcc ccacctttag cacgtcgatc
      961 acccgccgca ccgattcctc ggcgttgtcc tggacgtggc gcatgtaggc ctgcaccacg
     1021 tcgagcccgt agtgctccac cgcgcgggcg atttcggtca cacccttctg gttggccgcg
     1081 accatggccc tcaagtccgc gaggttctgc tccgggttgc gcgccgggta gcgcgcgccg
     1141 gcgaggaggg cgcgcgtctc gctttcgcgg aagcggcctt ccgataccag cttgaagttg
     1201 tcgatcagca cgccttcctc ttcaagatgg cggctgtcgg gcggcatcga gccgggcgtg
     1261 atgccgccga tgtcggcgtg gtgcccgcgg cacgccacgt agaacaggat gtctttgccg
     1321 gcggcgtcga aaaccggcgt gatcaccgtg agatcgggca ggtgcgtgcc accgtgatag
     1381 gggttgttca gcgtgtaggc gtcgccgggg cgtatcctgc cggcgttctc cgtcgcgacg
     1441 acgcgcacgc tctcgcccat ggcgccgagg tgaacgggga ggtgcggtgc gttggcgatc
     1501 aggccaccca cggggtcgaa cagcgcgcag gaaaaatcga gccgctcctt gatgttgacc
     1561 gaataggcgg tattggcgag cgtcacgccc atctgctcgg cgatcgccat gaagagattg
     1621 ttgaagattt cgagcatgac cggctctgcc tgcgtgccga ctgccgcccg gtgcggccgc
     1681 gcgacgcttc gagtcagcac caggtgattg agcggggtca cctgggcctg ccattgcggt
     1741 tcgatcacgg tcgtggcgtt cgcctccgcg atgatggtgg gaccggcgag gacg
//
LOCUS       DBNGSG010000060         3757 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_867416, whole genome shotgun sequence.
ACCESSION   DBNGSG010000060 DBNGSG010000000
VERSION     DBNGSG010000060.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_867416"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="AC158Q_01385"
     CDS             <1..536
                     /locus_tag="AC158Q_01385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3338451.1"
                     /translation="HLGLMAFAELCRRHPGARLTVIGRGPDEAWLRGLADDLGLAHAV
                     TWIPHLERAAVMQNYLHHDAFLFPSLHDSSGNVVLEALSSGVPVVCLDSGGPAVLVDP
                     SCGFKVRPGEPQQVVEDLAGALHALAKNPALRRAMGRAAASRAHEHFSWTRQVSRMEQ
                     LYFSVSPGPHPVAERAG"
     gene            557..2236
                     /locus_tag="AC158Q_01390"
     CDS             557..2236
                     /locus_tag="AC158Q_01390"
                     /inference="COORDINATES: protein motif:HMM:NF024627.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HIR3338452.1"
                     /translation="MNNLLSLLVLVIALAGGQAFAAIRYVDPTNPAAQDAGEGDVKRP
                     YRSLAYAVKRLQPGDTLNIAPGTYRESLVFPDMSWSGAPTSVQPVSKGAEVLIKGSDV
                     VGGWERVEPGVFVKRAWTVNSQQVFVDGKPLQQIGGTILNGYPDKPGHPMKNLHQGQG
                     GIWPGRVPGGLKEMTEDSFYYDAGAKSLYVKIAPAGALEGRTVEVSVRPYLVLGKGVK
                     ALNLSNLRFQHANTTAVSQSGAISLAGDRILLEGIEVSHVDGNGLDITGDENTVRASR
                     ANHCGQVGMKLRGGSAKLIDSETSFNNTRGFNTWWEAGGAKFVGHGGLHDSEIRGHRA
                     IGNNGDGIWFDWMNSNNKVHESVSAYNAGFGIHYEASQKGQIYNNRVFGNKLRGIYLP
                     HSSGSIVAHNLVAKNGMEGVVVVDEGRSKNKPELRPRGNRVIGNILAWNNRSAVVLPA
                     GLLENVSDYNLYLSGREPPGFSLGWASKASPVRKGLEAWRAASRQDMNSWSESFEIPK
                     ELLLALEKRQPDADWSGVMKLASRFSVLAADAAGGKPTAATSPQLTPGPRP"
     gene            2233..3408
                     /locus_tag="AC158Q_01395"
     CDS             2233..3408
                     /locus_tag="AC158Q_01395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3338453.1"
                     /translation="MSAPRVLVVHNRYQLPGGEDAVVEAEVGLLRAHGHEVATYVRDN
                     HELRDIKALDAFTQTLWSRRTSREIARLVAEFRPDVIHAHNTFALVSASVYWAAARAA
                     IPVVQTLHNFRLLCVQAMFLLEGRVCEDCLGRVPWRGVVRKCYRNSGLQSAALATMLG
                     MHRALGTYRDKVARYIALTDFCRAKFIEGGLPASRIVVKPNFVDVGHHPETAARRGGL
                     FVGRLAPEKGIDVLIEALRELPNVTFDVIGDGPERRKVESHPQLNSLGWLAPAEVHER
                     MRRAAYLVMPSIWYESFPRTLVEAYANGLPVVASHLGALAELVDHGRTGLLFEPGSAT
                     DLARHLAWAEAFPAKMRMMGERAREVYENRFTPGRNYEQLMAIYEDVIAGEKLRAVV"
     gene            3462..>3757
                     /locus_tag="AC158Q_01400"
     CDS             3462..>3757
                     /locus_tag="AC158Q_01400"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008194 - UDP-glycosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HIR3338454.1"
                     /translation="MAHGHDRVGASVLGSHIDAVGWGDCLDRIFAWASKRESRYVCMC
                     NVHSVVTARRDREFGEVLNGADLAPPDGAPVAWRLRALGHAGQQRISGPDLMWK"
BASE COUNT          644 a         1220 c         1319 g          574 t
ORIGIN      
        1 tgcacctcgg cttgatggca ttcgcggaat tgtgcaggcg tcaccccggc gcgcgcctga
       61 ccgtcatcgg ccgcggcccg gacgaggcgt ggttgcgcgg gctcgcggac gaccttggcc
      121 tcgcccatgc cgtgacctgg ataccgcacc tcgaacgcgc ggcggtcatg cagaactatc
      181 tgcaccatga cgcgttcctg ttcccgagcc tgcacgactc cagcggcaac gtggtgctgg
      241 aagcgctttc cagcggggtg cccgtcgtgt gcctcgattc cggcgggccc gccgtgctgg
      301 tggatccttc ctgcggattc aaggtccgcc cgggcgagcc gcagcaggtg gtggaggacc
      361 tggcgggtgc gctccatgcg cttgcgaaaa atcccgcgct gaggcgcgcg atggggcgag
      421 cggcggcgag ccgggcgcac gaacactttt cgtggacgcg ccaggtttcg cgcatggagc
      481 agctgtactt ctccgtcagt cccggtccgc atccggtcgc ggagcgcgcg ggctgacgaa
      541 tggaggcgaa tctgcgatga ataacctctt gagcctgctg gtgctcgtga tcgccctggc
      601 aggcggacag gcctttgccg cgatacgtta cgtcgatccc acgaatcccg cggcacagga
      661 cgcgggcgaa ggggacgtga agcgccccta ccgctcgctc gcctacgccg tgaagcggct
      721 gcagcccgga gacacgctga atatcgcgcc cgggacgtac cgggagtcgc ttgtttttcc
      781 ggacatgagc tggtcgggag cgcccaccag cgtgcagccc gtgagcaagg gagcggaagt
      841 gctcatcaag gggtccgacg tggtcggcgg ctgggagcgg gtggagcccg gcgttttcgt
      901 caagcgcgcg tggaccgtca acagccagca ggtcttcgtg gatggcaagc cgctccagca
      961 gatcggcgga accatattga acggctatcc cgacaagccc ggtcacccga tgaagaacct
     1021 gcaccagggc cagggcggaa tctggccggg acgcgtgccg ggcggcctca aggaaatgac
     1081 ggaagacagc ttttactacg acgccggcgc aaagagcctg tacgtgaaaa tcgcgccggc
     1141 cggcgcgctc gagggccgca ccgtcgaagt cagcgtgcgg ccgtacctcg tgctgggcaa
     1201 gggcgtgaag gcgctcaatc tctccaacct gcgcttccag cacgcgaaca cgaccgccgt
     1261 gagccagtcc ggcgcgatat cgcttgccgg cgaccgcatc ctgctcgaag gcatcgaggt
     1321 gagccacgtg gacggcaacg ggctcgatat cacgggcgac gagaacaccg tcagggcgag
     1381 ccgggcgaac cactgcgggc aggtcggaat gaagctgcgg gggggcagcg cgaagctgat
     1441 cgacagcgag accagcttca acaatacgcg cggattcaac acgtggtggg aagccggcgg
     1501 cgcgaagttc gtcggtcacg gcgggctgca cgactccgag atacgcggcc accgcgcgat
     1561 cggcaacaac ggcgacggca tctggttcga ctggatgaac agcaacaaca aggtccacga
     1621 gagcgtgtcg gcgtacaacg cggggttcgg catccattac gaggcgagcc agaagggcca
     1681 gatctacaac aaccgcgtgt tcggcaacaa gctgcgcggg atctacctgc cgcacagctc
     1741 cgggtccatc gtcgcgcaca acctcgtcgc gaagaacggc atggagggcg tcgtcgtcgt
     1801 cgacgagggg cgcagcaaga acaagccgga actgcgcccg cgcggcaacc gcgtcatcgg
     1861 caacatcctc gcctggaaca accggtccgc cgtcgtcctg ccggcagggc tgctcgagaa
     1921 cgtctccgac tacaacctct atctgagcgg ccgcgagccg ccggggtttt cgctgggctg
     1981 ggcgtcgaag gcgagccccg tgagaaaagg cctcgaggcc tggcgcgcgg cgtcccggca
     2041 ggacatgaac tcgtggagcg agtccttcga gattccgaag gaactgctgc tcgcgctcga
     2101 gaaacgccag cccgacgccg actggtcggg cgtgatgaag ctcgcgtcga gattcagcgt
     2161 gctggccgcc gacgcggcgg gtggaaagcc gacggccgcc acttcgccgc aactgacgcc
     2221 ggggcccagg ccatgagcgc gccgagggtc ctcgtcgtgc acaaccgcta ccagcttccc
     2281 ggcggggagg atgccgtcgt cgaggccgag gtcgggctgc tgcgcgccca tggccacgaa
     2341 gtcgcgacct acgtgcgcga caaccacgaa ttgcgcgaca tcaaggcgct cgatgccttt
     2401 acgcagacgc tgtggtcgcg ccgcacgtcg cgcgagatcg cccggctcgt ggccgaattc
     2461 cgcccggacg tcattcatgc ccacaatacc tttgccctgg tatcggcctc ggtctactgg
     2521 gcggccgccc gcgccgcgat cccggtggtg cagacgctgc acaatttccg cctcctgtgc
     2581 gtgcaggcga tgttcctgct cgaaggccgg gtgtgcgagg actgcctggg gcgcgttccc
     2641 tggcggggcg tcgtgcgcaa gtgctaccgc aactcggggc tgcagtcggc ggcgcttgcg
     2701 acgatgctgg gcatgcatcg cgccctcggc acctatcgcg acaaggtggc gcgctacatc
     2761 gcgctcacgg atttctgccg ggccaagttc atagagggcg ggctccccgc gtcgcgcatc
     2821 gtggtcaaac ccaatttcgt ggacgtcggg catcacccgg aaacagcggc ccgccgcggc
     2881 ggcctcttcg tcgggcggct ggcgccggaa aagggcatcg acgtgctgat cgaggcgctg
     2941 cgcgagctgc ccaacgtcac gttcgacgtg atcggcgacg gccccgagcg ccgcaaggtc
     3001 gagtcccacc cgcagttgaa ttcgctgggg tggctcgcgc ccgccgaggt ccacgagcgc
     3061 atgcgccggg cggcgtacct ggtcatgccg agcatctggt acgaaagctt tccccgcacg
     3121 ctggtcgagg cgtacgccaa cgggctgccg gtggtcgcca gccacctcgg cgccctggcg
     3181 gagctggtgg atcacggccg caccggcctg ctgttcgagc cgggatcggc gaccgatctg
     3241 gcgcgccacc tggcatgggc ggaggcgttc cccgcgaaga tgcggatgat gggggagcgc
     3301 gcgcgcgagg tctacgagaa ccgctttacg cccggcagga attacgagca gttgatggcg
     3361 atctacgagg acgtcatcgc cggggagaag ctgagggccg tggtctagtg tcccgaagat
     3421 gacggattca tcaccgaact agcgactcag gataccagcg catggcgcac ggacacgaca
     3481 gggtcggggc gagcgtgctc ggatcgcata tcgatgcggt cggctgggga gactgcctgg
     3541 accggatctt cgcctgggcc tcgaagcggg aatcgcgcta cgtctgcatg tgcaacgtgc
     3601 attccgtcgt gaccgcccgc cgtgaccggg aattcggcga ggtgttgaac ggcgccgacc
     3661 tggccccgcc cgacggggcg ccggtggcct ggcgcctgcg cgcgctgggc catgccggac
     3721 agcagcgcat cagcggcccg gacctgatgt ggaaatg
//
LOCUS       DBNGSG010000061         3759 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1547032, whole genome shotgun sequence.
ACCESSION   DBNGSG010000061 DBNGSG010000000
VERSION     DBNGSG010000061.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1547032"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..1060
                     /locus_tag="AC158Q_01405"
     CDS             134..1060
                     /locus_tag="AC158Q_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3338455.1"
                     /translation="MPPVISISKLGKTYASGLRALDSVDLEIRKGEIFALLGPNGAGK
                     TTLINIICGIVTPTSGTVRVDGHEIRRDYRATRSKIGLVPQELHTDMFETPWGTVRFS
                     RGLFGRAPGPAQLEKVLRSLSLWEKRHDKIMTLSGGMKRRVLIAKALAHEPEILFLDE
                     PTAGVDVELRRDMWGLVHALRASGVTIILTTHYIEEAEEMADRIGVISKGSLILVEEK
                     AALMKKLGKKQLTLQLQDPLAEIPAGLGEWRLVLKGGGTELEYTFDAGETRAGIASLL
                     RRMGELGIAYKDLHTSQSSLEDIFVSLVSEKS"
     gene            1057..1830
                     /locus_tag="AC158Q_01410"
     CDS             1057..1830
                     /locus_tag="AC158Q_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014251249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3338456.1"
                     /translation="MNPGFNRHAVLAIFRFELARFRRTLWQSLVTPVLTTSLYFIVFG
                     AAIGPSMGEVGGVPYGAFIVPGLIMLSLFTESLNNASFGIYLPRFTGTIYEVLSAPVS
                     PIEMVLGYVGAAATKSVMLGLIILATAGLFVPIQIAHPLWMIAFLLLTAATFSLFGFI
                     IGIWANGFEQLQFIPMLVITPLTFLGGAFYSIDMLPSAWRTFSLFNPVVYLISGFRWS
                     FYGKSDVGVAVSLAATLGFFLACLVVVAWIFRTGYRLKN"
     gene            1886..2320
                     /locus_tag="AC158Q_01415"
     CDS             1886..2320
                     /locus_tag="AC158Q_01415"
                     /inference="COORDINATES: protein motif:HMM:NF019928.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3338457.1"
                     /translation="MDTAPYIEVRVAQRFSVPPEQVFDAWVDPAAAGKWLFATAWRPM
                     SRVAIDARAGGTFRLEERPGGKGVVQAGRYLEFDRPRRLVFTLLDGNGRRDTPRVSVG
                     IVALEAGCELTLVHEGVLPPDANRVEGRWLGMLHGLATLLER"
     gene            2488..2922
                     /locus_tag="AC158Q_01420"
     CDS             2488..2922
                     /locus_tag="AC158Q_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338458.1"
                     /translation="MRQEIGIALAMFFGGMPLAATPAEPDAETRAAARLIASVEQPLA
                     KRDLKGYCAAVYGTPDYAKFVANACQAGIKKKVKKPEDCSDANLKQEIGRIIGSCVAM
                     PPDQFDLTVKRWSEVRGKFLVEMTAKGIDGEKTLKEERAKLR"
     gene            3023..3487
                     /locus_tag="AC158Q_01425"
     CDS             3023..3487
                     /locus_tag="AC158Q_01425"
                     /inference="COORDINATES: protein motif:HMM:NF017999.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HIR3338459.1"
                     /translation="MIEVNERIEVPSTPRTVWDLLSNPQAVVECVPGAALGDRHEDGS
                     YDASMVVKFGPAKVTFRARFTLELDEAAMAGQVASKARDNQGGTRVKTAMNFKVVEGA
                     APGSAAILIDAQVEISGKLASLVESGANLVVKHMTREFSERLAARVAGAAAA"
     gene            3597..>3759
                     /locus_tag="AC158Q_01430"
     CDS             3597..>3759
                     /locus_tag="AC158Q_01430"
                     /inference="COORDINATES: protein motif:HMM:NF014795.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR3338460.1"
                     /translation="MKVYQRLAHAFVAEGATNMFGIMGDGNMYWMHELDKLGGVKMHE
                     VRHEGAGLGM"
BASE COUNT          709 a         1151 c         1248 g          651 t
ORIGIN      
        1 cgcgccaaga caggatcgtg ataagtaaca agtaacaagt aacaagtgac atgtgacagg
       61 tgactagttt tccggcgaac gccgggagcc ctgttcccga cttccgactt tcgactttct
      121 acttgtaatt gacatgccgc cggtcatctc gatctccaag ctgggcaaga cctacgcctc
      181 gggcctgcga gcgctggata gcgtcgacct cgagatccgg aaaggcgaga tcttcgcgct
      241 gctggggccc aacggcgcgg gcaagaccac gctgatcaac atcatctgcg ggatcgtcac
      301 cccgacatcg ggaacggtgc gggtcgacgg ccacgaaatc cggcgcgact accgcgccac
      361 ccgctcgaaa atcggcctgg tcccgcagga gctgcacacc gacatgttcg aaacgccgtg
      421 ggggacggtc cgcttcagcc gcgggttgtt cgggcgcgca ccgggcccgg cgcaactcga
      481 gaaggtgctg cggagtctct cgctgtggga aaagcgccac gacaagatca tgacgctgtc
      541 gggcggcatg aagcggcgcg tgctgatcgc caaggcgctc gcgcacgagc cggagatcct
      601 gttcctcgac gaaccgacgg cgggcgtgga cgtggagttg cgccgcgaca tgtggggcct
      661 ggtgcacgcc ctgcgcgcga gcggcgtcac catcatcctg accacgcact acatcgagga
      721 ggcggaggaa atggcggacc gcatcggcgt catcagcaag ggcagcctga tcttggtcga
      781 ggagaaggcg gcattaatga agaagctcgg caagaagcag ctcaccctcc agctgcagga
      841 cccgctggcg gagattcccg ccgggctggg tgaatggcgc ctcgtcttga agggcggcgg
      901 caccgagctg gaatacacct tcgacgccgg ggagacgcgc gccggcatcg cgtcgctgct
      961 gcgccgcatg ggcgagcttg gcatcgccta caaggacctc cataccagcc agagctcgct
     1021 cgaggacatt ttcgtcagcc tggtgagcga aaaatcatga acccgggctt caaccgtcac
     1081 gccgttctgg cgatattcag gttcgagctg gcgcggttcc ggcgcacgct gtggcagagc
     1141 ctggtgacgc cggtgctcac cacgtcgctg tacttcatcg tcttcggcgc cgccatcggc
     1201 ccgagcatgg gcgaagtggg aggcgtgccc tacggcgcgt tcatcgtccc cgggctgatc
     1261 atgctgtcgc ttttcacgga gagcctcaac aatgcctcct tcggcatcta ccttccgaga
     1321 ttcaccggca ccatctacga agtgctctcc gcgccggtat cgccgatcga gatggtcctt
     1381 ggctacgtcg gagcggcggc gaccaagtcg gtcatgctcg ggctgatcat actcgccacg
     1441 gcgggcctct tcgtgccgat ccagattgcg caccccttgt ggatgatcgc gttcctgttg
     1501 ctgacggcgg cgacattcag tctattcggc ttcatcatcg gcatctgggc gaacggcttc
     1561 gagcagctcc agttcatccc catgctggtg atcaccccgc tgaccttcct cggcggcgcc
     1621 ttctactcga tcgacatgct cccgtccgcg tggcgcacgt tcagcctctt caacccggtg
     1681 gtctacctga tcagcggctt ccgctggagc ttctatggaa aatcggatgt cggagtcgcg
     1741 gtgagtctgg cggctaccct cggctttttc ctcgcctgcc tggtcgtggt ggcctggatt
     1801 ttccggaccg ggtaccggct gaagaactga aggacgatcc ccgcagtgcg ggagactcat
     1861 cacccatcac tcatcactta ctggcatgga taccgcaccg tatattgaag tccgcgtcgc
     1921 gcaacgcttc agcgtgccgc cggaacaggt tttcgacgcc tgggtcgatc ccgctgccgc
     1981 gggtaaatgg ctgttcgcga cggcgtggcg cccgatgtcc cgggtcgcga tcgatgcgcg
     2041 cgccggtggc acgttccgcc tcgaggaacg gcccggaggg aagggtgtcg tccaggcggg
     2101 caggtacctc gaattcgaca ggccccggcg cctcgtcttc accttgctgg acgggaatgg
     2161 ccggcgggac acgccccgcg tgagcgtcgg catcgttgcg ctggaggcgg gatgcgagct
     2221 caccctggtc cacgagggcg tgctcccgcc cgatgcaaac cgcgtcgagg gccgctggct
     2281 cggcatgctc cacgggctgg cgacgctgct ggaacggtaa cgaagcgagg gtttgaaggc
     2341 gcactgcggg agccgcgtca cccatcacgg gcgcagcgct tccccgggcc cggcattggt
     2401 accagtagaa tacgaattcc tccctatgga acgataggcc gggaaagaga tgtcttacgt
     2461 caccccctgc gggtgcgagg agttcgcatg aggcaggaaa tcggaattgc gctggccatg
     2521 ttcttcggcg gcatgccgct tgccgcgaca ccggcggagc cggacgccga gacgcgcgcg
     2581 gcagcccggc tgatcgcaag cgtggaacag ccgctggcga agcgcgacct gaagggctat
     2641 tgcgcggcgg tgtacggcac gccggattac gccaagttcg tcgcgaatgc ctgccaggcc
     2701 ggcatcaaga aaaaagtcaa gaagccggaa gactgctcgg atgcgaacct caagcaggaa
     2761 atcggaagga ttatcggcag ctgcgtcgcg atgccgcccg atcagttcga tttgacggtg
     2821 aaacgctgga gcgaggtgcg cgggaaattc ctcgtggaaa tgacggccaa gggcattgac
     2881 ggcgagaaga ccctgaagga agaacgcgcg aagctgcgct gaccggcgct ggttggtcat
     2941 atcttggact ccttgctccc tgcactgagt aacgggatac tgtggccccg tcacccatca
     3001 ccgcagcaaa tcggattcca gcatgatcga agtcaacgag cggatcgaag tgccgtcgac
     3061 gccgcggacg gtatgggacc tgctgtcgaa cccgcaagcc gtggtcgagt gcgtgccggg
     3121 cgcagcgctg ggggaccggc acgaggatgg gtcttacgac gcgtcgatgg tcgtcaagtt
     3181 cgggcccgcc aaggtcacgt tccgcgcgcg cttcacgctg gagctggacg aggccgcgat
     3241 ggccggccag gtcgcctcga aggcccgcga caaccagggc ggcacgcgcg taaagaccgc
     3301 catgaatttc aaggtcgtgg aaggagcggc gccgggctcg gccgccatac tgatcgacgc
     3361 ccaggtggag atttccggca agctcgcttc gctggtggaa agcggagcga atctggtggt
     3421 gaagcacatg accagggagt tctcggagcg gctcgcagcc agggtcgcgg gcgcggccgc
     3481 tgcctgatga cggaagtcga aagccgcgag tcggaaggcg aaagtaaaag cggacaagtc
     3541 gtatttgatc tgtatccatc actcatcacc catcacgcga gcaagggaga ggaagcatga
     3601 aggtctatca gcggctggcc cacgcattcg tcgcggaagg cgcgacgaac atgttcggca
     3661 tcatgggcga cggcaacatg tactggatgc acgagctcga caagttgggc ggcgtcaaga
     3721 tgcacgaagt gcgccacgag ggcgccgggc tgggcatgg
//
LOCUS       DBNGSG010000062         7755 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2395985, whole genome shotgun sequence.
ACCESSION   DBNGSG010000062 DBNGSG010000000
VERSION     DBNGSG010000062.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7755)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7755)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7755
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2395985"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="AC158Q_01435"
     CDS             <1..325
                     /locus_tag="AC158Q_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338461.1"
                     /translation="AIEHYFVSGAQPLRIGGKFRNNSSFEWESIKISAHIYLDGKYAA
                     RCGANSVTLDANSTLYQVRARTEHHFAAICHDFGLDRPYSDVTYKLLVEGNRKGEQAP
                     KTERK"
     gene            804..1718
                     /locus_tag="AC158Q_01440"
     CDS             804..1718
                     /locus_tag="AC158Q_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HIR3338462.1"
                     /translation="MERWNLGNMGLGSTVVAALLLPGGILLLAWVLYRRYGSRLAGRW
                     VALAALIAALQGCATQLAIRPAPGPDDQDAASTRQVLLFQSSSIAPKNNDAPLLPEGL
                     RQGDIILTSDPGFVSASIQLMTLAPVSHAAIYIGDGKVVEAVRPAVRVRALDEVMAEG
                     TVVLAFRHPELSTEQARNISNYALQKTGTGFNYFGVTLHVPFSITRRLCELPLLPSPL
                     RDACLRGIGALHYLAAAETQTFCSQLVLDAYRQAGVPITDADPRLISPADILHMREGD
                     VSSVRIHKSLRYVGHLKYVEPTVIALQQ"
     gene            complement(1782..3020)
                     /locus_tag="AC158Q_01445"
     CDS             complement(1782..3020)
                     /locus_tag="AC158Q_01445"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3338463.1"
                     /translation="MYRSRRKLIAAACGAAALGGCSFSFEHGLLNPCRGESPNDPAIE
                     AAVLAAWEGIDATLVWDCHVHLVGEGDSGSGIWLHPAMRSVMRPLLYAQRLLYRNASC
                     SGVEPGPVDDRYVERLQRLIEELPPGAKAVLLALDWHHTEDGEPLPAATAIRIPGEYA
                     MSIAARHRGRFEWAASIHPYRRDCIAALRAAKANGACAVKWLPATMGIDPGSGRCDAF
                     YAEIARLGLPLITHAGTESAVRFGEQQHLGNPLRLRRPLEHGVRVVVAHCATLGEDRD
                     LDRGEDGPYVPSFELFARLMDDARYRENLYGDLSAVAQMNRAGRFLKRLLERRDWHAR
                     LLNGSDYPLPGVMPIHSLNLLVQLEVIEPAIAPVLAGIREYNPLLFDFVLKRHLRAGA
                     ARFPAGVFETRPFFQVSGVS"
     gene            3243..3437
                     /locus_tag="AC158Q_01450"
     CDS             3243..3437
                     /locus_tag="AC158Q_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338464.1"
                     /translation="MGTRSEQRRARIPAPHEDKTTGLAEIAFEEEDETPCSEYVVMSQ
                     ERDLPALEEYLREHGSRTIH"
     gene            3479..3916
                     /locus_tag="AC158Q_01455"
     CDS             3479..3916
                     /locus_tag="AC158Q_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338465.1"
                     /translation="MVRTILIAGCLVFTGCMTAPQHRAAVNDDPADRLTVGRVQKEIR
                     VGMSGADVAAILGSPNIVSTDEQRREVWIYDRIATESVYSSSYGGISALILGLGGNVA
                     GGAAPGASYSAGASAASQRTFTVIIKYDHERKVRDFAYHTSRF"
     gene            3921..4454
                     /locus_tag="AC158Q_01460"
     CDS             3921..4454
                     /locus_tag="AC158Q_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338466.1"
                     /translation="MRRSHLIAAAVLAVAATGCVHLPDSALALSPGTLERRQIETRRF
                     DGIAEADLFAACAGVLQDLGFSVESSDSRLGLLTASKQRGAVNAGQIAGAIVIGLLTG
                     AMIPTDRDQTIRATVIVFPAPGEKKGNYFVRIGLQRAVRTTDPGVTRREWLKDPALYQ
                     GFFAKLSQSIFLEAHKI"
     gene            4451..4882
                     /locus_tag="AC158Q_01465"
     CDS             4451..4882
                     /locus_tag="AC158Q_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338467.1"
                     /translation="MNPRIARLTVLAAAFAALGGCALTGSQREVLATDQSQVAQRAYQ
                     TRAFDTTDQEQTLRAIIATLQDFGFIIDRADPVLGIVSGTKMDGYHMRITVSVRPRGT
                     TQVLVRANAQHQIKAVTDPKPYQNFFVALERSMFLKAHEVE"
     gene            4928..5479
                     /locus_tag="AC158Q_01470"
     CDS             4928..5479
                     /locus_tag="AC158Q_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338468.1"
                     /translation="MKIRNPVPSVFSHSTLNRVISARLALALAAPAVALSGCYVMPVG
                     QDSSGNPYYAYSPLPIPPVVSSQGAARGAHPAAVPGGPIAATLGVKLYPINDLANQTG
                     VLTGQVTNMMNGQGRFAFNYQGETLVGEATRVANDERRGIASAYGGRGTFARCEYQMS
                     TPLLGAGTCTFSNGAQYQLHVGS"
     gene            5595..6209
                     /locus_tag="AC158Q_01475"
     CDS             5595..6209
                     /locus_tag="AC158Q_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338469.1"
                     /translation="MMFSNQLAGVGREKRTAGTWLVALALAFGSALMQGCATPASSVN
                     MVAKVDTRAPREALLKEGITVRNVTGGSDTNPLWMSKVSNDDFKKALEDSLQSARLHN
                     GGVPARYQLDATLLKLDQPMFGLDLTVTCSTQYDLLETGSGNRVFTRTITTPFTARVS
                     DSFLAIERLRIANEGAVRENIQHFLREILDLDFARTATATPPAR"
     gene            6252..6698
                     /locus_tag="AC158Q_01480"
     CDS             6252..6698
                     /locus_tag="AC158Q_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338470.1"
                     /translation="MKANRLLALGAALLPAVTGCASITGSTMQPMTVETRDKGGKEVT
                     GAACDLNNNKGKWSVTTPGSVVITRSNDNLLVVCQKEGHETGNASVVSTVKGALFGNI
                     IFGGGIGAIIDHASGSAYEYPQLIPIEMGSVRVIQAPREPETPQQP"
     gene            6960..>7755
                     /locus_tag="AC158Q_01485"
     CDS             6960..>7755
                     /locus_tag="AC158Q_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR3338471.1"
                     /translation="METSVARQVGGAGGAAISADALIETLRQLAAELHPSRASIPSSL
                     DARLEQDYGFDSLGRVELFLRIERRFGTSLTETVMASAETPRDLLRAMLAASPHQVHP
                     AALERTTALASESQTPDDAATLPEVLEWHVRKHPSRPHIVLQDDEERERVVTYAELDQ
                     AARAVAAGLIERGLEPGKAVAIMLPTGVEYFYSFFGILLAGGIPVPIYPPARPSQIED
                     HLRRHAGILSNALAAALITVPQAKPLALLLKPRVDSLREVVTPEELM"
BASE COUNT         1484 a         2550 c         2411 g         1310 t
ORIGIN      
        1 agcgatcgaa cactacttcg taagcggtgc tcaaccactg aggattggcg gtaagttcag
       61 gaataattcc agcttcgaat gggaaagcat caagatcagt gctcatatct atctcgacgg
      121 gaaatacgcc gcgcgatgcg gcgcgaattc tgtaaccctt gatgcaaaca gcaccctata
      181 tcaagtgcgc gctcgcactg aacatcactt tgcagccata tgccatgact tcggcttgga
      241 caggccgtat tccgatgtga cgtacaagct cttggtcgaa gggaacagaa aaggtgagca
      301 agctccaaaa acggaaagaa agtaaccacc gttcgattga ttgcaggtag cgagccgcca
      361 ggcatccacc cagggggtgt cgcagccatt ggatagtgct gggcgctaac actcgtcaaa
      421 gctctactcc tctggcgcaa tcttgtaatg cgcacagcac gggaaatcgc cgccgaggta
      481 ttcctgcttg gcagctgcct gcagtgcgcg cttttttact tgacgatctt cggttggaga
      541 ccgcttttgc aactggaggt gtacgcactc gctgtcctaa tcgcttgcac cgattgcgtc
      601 gtattcatcc tgttggcctt gggaactgcc gatcccgcca tagcggccaa gtcctcctct
      661 taacggactg ttttaaataa gaattatcca tacgttctgc cccgcaactg taagtcatcc
      721 gtaacccacc cgtaagccat tcggatgcgg ctcccccgca tcctgccccc agtgaaaccg
      781 cgcactgatc aagggagggg gacatggaac ggtggaacct cgggaacatg ggtctgggca
      841 gtaccgtcgt cgcagcgctg ctattgccgg gcggaatcct gttgctcgcc tgggtgcttt
      901 atcgccgata cgggtcccgg cttgcgggac gctgggttgc acttgcagcg ctcatcgccg
      961 ccttgcaggg ctgtgcgacg caactcgcca tccgcccggc gcccggaccg gacgatcagg
     1021 atgccgcgtc cacgcgtcag gtcctgttgt tccagagttc cagcatcgcg cccaaaaaca
     1081 acgatgctcc gctgcttccg gagggcttgc gtcagggcga catcatactg acctcggacc
     1141 cgggctttgt ctccgccagc attcaactga tgactttggc gccggtgagt cacgccgcga
     1201 tctacattgg cgacgggaag gtcgtggagg cggtgcgccc ggcggtgcgg gtccgggcgc
     1261 ttgacgaagt gatggccgaa gggaccgtgg tactcgcgtt ccggcatccc gaactcagca
     1321 cggagcaggc ccggaatatc agcaattacg cgctgcagaa gacgggcacc ggtttcaact
     1381 acttcggcgt gacgctgcac gtgccatttt cgattacgcg ccggttgtgc gaactgcccc
     1441 tcttgccctc gccgctccgg gacgcgtgcc tcaggggaat cggggcgctt cactacctgg
     1501 ccgccgcgga aacccagact ttttgttcgc agctggtgct ggatgcctat cggcaagccg
     1561 gggtgcccat caccgacgcg gacccgcgcc tgatcagccc ggccgacatc ctgcacatgc
     1621 gcgaaggcga tgtgtcctcg gtcaggatcc acaagtcgct gcgctacgtt ggtcatctga
     1681 agtacgtgga gccgacggtc atcgccctgc agcaatgaca cggctgcgca tacaccacca
     1741 gcttgctggt acagcactgg tttccggatg cgttccaggg gtcaggacac tcccgatacc
     1801 tgaaagaaag gccgtgtttc gaatacccct gccggaaagc gtgctgctcc cgcgcgcagg
     1861 tggcgtttga gcacgaagtc gaacagcagg ggattgtatt cacgaattcc ggccagtacc
     1921 ggcgcgatcg caggctcgat aacctcgagc tgaaccagca ggttcaatga atggatcggc
     1981 atgacaccgg ggagcggata atcggagccg ttgagcagcc gcgcgtgcca atcccggcgt
     2041 tccagcagtc ttttcagaaa ccggccggca cggttcatct gcgcgacggc cgacaagtcg
     2101 ccgtagaggt tctcccggta gcgcgcgtcg tccatcagcc gcgcaaacaa ctcgaagctc
     2161 ggaacgtaag ggccgtcttc cccgcggtcg agatcgcggt cttcacccag ggttgcgcaa
     2221 tgcgcgacca cgacgcgcac gccgtgctcc agggggcggc gcagccggag gggattgccc
     2281 agatgctgct gttcgccgaa tcgcaccgcg ctttccgtgc cggcatgggt gatgagcggg
     2341 aggccgagtc ttgcgatttc ggcgtagaac gcgtcgcacc ggccggagcc ggggtctatc
     2401 cccatcgtcg ctggcagcca tttgaccgcg cacgcgccgt ttgccttcgc cgcgcgcagg
     2461 gcagcgatgc agtcgcgccg ataaggatgg atggaggcgg cccattcgaa gcgcccccga
     2521 tgccgcgctg cgatgctcat cgcatactcg ccgggaatgc ggattgcggt agccgccgga
     2581 agcggttcgc cgtcctcggt atggtgccag tccagtgcca gcagaacggc tttcgcgccc
     2641 ggcggcagtt cctcgatcag gcgttgcaag cgctcgacat atcgatcgtc aacggggcca
     2701 ggctccacgc ccgagcaact ggcgttgcga tagagcagcc gctgcgcgta caagaggggg
     2761 cgcatgacgc tccgcatcgc agggtggagc cagatcccgc ttccggaatc gccctcgcct
     2821 acgagatgga cgtgacagtc ccagaccagc gtcgcgtcga taccctccca cgccgccagc
     2881 acggcggcct cgatggcggg atcgttcggt gattccccgc ggcagggatt caacaggcca
     2941 tgctcgaacg agaagctgca gccgcccagc gcggcggccc cacatgcagc tgcgattagt
     3001 ttccggcgtg atcggtacat ggcatggcgg cggctcttat gttaaccccc gccaacgccg
     3061 ggtgcgtacc gcacaagtct tataaaacac atatatcaca ttgatatata tgaagaatac
     3121 atgggcggca tgcgtaagcc atttgtaacc catcggtaag cgatgtggat gcgccccggc
     3181 agcatcctgt gcgccatgcc gcaaggaacc cgagcgcggc tctacacaaa tggaggtgtg
     3241 acatgggaac cagaagcgaa cagcgccgcg cccgaatccc ggcgccgcac gaggataaga
     3301 cgacaggtct cgccgaaatc gccttcgagg aagaggacga aactccctgc agcgagtacg
     3361 tcgtcatgag ccaggaacgc gacctccccg cactcgagga atacctgcgg gagcacggct
     3421 cgcgcacgat tcattgatcg tccacgaaac cgcaacccgc ccaccggagg ccatccatat
     3481 ggtacgaacc atcctgatcg cgggctgcct cgtgttcacg ggctgcatga ccgcgcccca
     3541 gcaccgcgcg gcggtgaatg acgatccggc cgaccgcctg acagtcgggc gcgtccagaa
     3601 ggaaatccga gtcggcatga gcggcgcgga cgtcgcggcg atcctgggat cgccgaacat
     3661 cgtgagcacg gatgagcagc gccgcgaggt ctggatctac gaccggatcg ccaccgagtc
     3721 ggtttattcc agcagctacg gcggcatcag cgcgctcatc ctcggcttgg gcgggaacgt
     3781 ggccggcggc gcggctccgg gcgcttcgta cagcgccggc gccagcgcgg catcccagcg
     3841 cactttcacc gtgatcatca aatacgatca cgagcggaag gtgcgcgatt ttgcctatca
     3901 cacatcgcgc ttctagggcc gtgcgccgct cccatctcat cgccgccgcc gtgctggccg
     3961 tcgccgcaac gggctgcgtg cacctgcccg acagcgcgct ggccctgagc ccgggcacgc
     4021 tcgaacggcg ccagatcgaa acgcggcgct tcgacggcat cgccgaggcg gacctgttcg
     4081 ccgcctgcgc cggggtgctc caggacctgg ggttcagcgt cgagtcatcg gacagccggc
     4141 tcggcctgct gaccgcctcg aagcagcgcg gcgcagtcaa cgccggccag attgcggggg
     4201 ccatcgtcat cggcctgctg accggcgcga tgataccgac ggacagggac cagaccattc
     4261 gcgccaccgt gatcgtgttt ccggccccgg gagagaagaa aggcaactac ttcgtccgca
     4321 tcggcctgca gcgcgccgtg cgcacgaccg atcccggggt cacccgcagg gaatggctga
     4381 aggaccccgc gctgtaccag ggcttcttcg cgaagctgtc gcaatccatc ttcctggagg
     4441 cacacaagat atgaaccccc gcatagccag attgacggtc ctggccgccg cgttcgccgc
     4501 tctcggcggg tgcgcgctca ccggttcgca gcgagaggtc ctggcaaccg accagagcca
     4561 ggttgcgcag cgcgcctacc agacgcgcgc tttcgacact accgaccagg agcagacgct
     4621 gcgcgccatc atcgccacct tgcaggattt cggtttcatc atcgaccggg ccgacccggt
     4681 gctgggcatc gtgagcggca ccaagatgga tggctatcac atgcgcatca cggtatcggt
     4741 caggccgcgg ggaacgaccc aggtgctggt gagggcgaat gcgcagcacc agatcaaggc
     4801 ggtgaccgat cccaagccgt accagaactt cttcgtcgcc ctggaacggt cgatgttcct
     4861 gaaggcccac gaagtcgaat gagtgaccga agcaaagcgt cttattaacc cagtgaaagg
     4921 agaccctatg aagatccgca atcccgttcc gtcagtgttt tcgcacagca ctttgaaccg
     4981 cgtcatctcc gcgaggctgg cgctggcgct ggccgcgccg gccgtggcgc tctccggctg
     5041 ctacgtcatg ccggtcgggc aggattccag cggcaacccg tactatgctt actcgccgct
     5101 gccgattccg cccgtcgtgt cctcgcaggg cgccgcccgc ggcgcgcacc ccgccgcggt
     5161 cccaggcggg ccgattgcgg cgacgctcgg ggtcaagctc taccccatca acgatctcgc
     5221 caaccagacc ggcgtgctga ccggacaggt gacgaacatg atgaacggcc agggccgctt
     5281 cgctttcaac taccagggcg agacgctggt cggtgaagcc acgcgcgttg ccaacgacga
     5341 gcggcgcggc attgcgagcg catacggcgg ccgcggcacg ttcgcgcgct gcgagtacca
     5401 gatgagcacc ccgttgctgg gcgccggcac ctgcacgttc tccaacggcg cgcagtacca
     5461 gctgcacgtc ggcagctgag cgtagcgcct gcacgcccgg gcaggaccgg gaaacctgag
     5521 caggccacag ccctcctggc cggccgggtt gacgacgcgg ggggcacatg acaatccatg
     5581 caaggagaac cgacatgatg ttttcaaacc aactggcagg cgtgggacgc gagaagcgga
     5641 ccgctggaac gtggctcgtt gcactcgccc tggctttcgg atcggccctg atgcagggct
     5701 gcgccacgcc cgcctccagc gtcaacatgg tggccaaggt agatacccgc gccccgcggg
     5761 aggcattgct gaaggaagga atcaccgtcc gcaacgtcac tggcgggtcc gacaccaacc
     5821 cgttgtggat gtccaaggta tcgaacgacg atttcaagaa ggcgctcgag gattccctcc
     5881 agtccgcccg gctgcacaac ggaggcgtgc cggcgcgcta ccagctggat gcgactctgc
     5941 tgaagctcga ccagccgatg ttcggcctcg acctcacggt gacctgctcc acgcagtatg
     6001 acctgctcga gaccggatcc gggaaccggg tattcaccag gaccatcacc acgccgttta
     6061 ccgcgcgcgt cagcgactcg tttctcgcca tcgagcggct gcgcatcgcg aacgagggcg
     6121 cggtgcgcga aaacatccag catttcctgc gggagattct cgacctcgat ttcgccagga
     6181 cagcaacggc aaccccgccg gcacgctgat cgtgccttga gggcgataac catcacagaa
     6241 gggaggcctg catgaaggcg aatagactgc ttgcgctggg cgcggccctg ttaccggccg
     6301 tcacgggctg cgcatcgatc accggatcga ccatgcaacc catgaccgtt gaaacccgcg
     6361 acaaaggcgg caaggaagtt accggcgcgg cgtgcgacct caacaacaat aaaggcaagt
     6421 ggtcggtgac gacgccgggg tccgtcgtca tcacccgctc caacgacaac ctgctggtcg
     6481 tatgccagaa ggaaggccac gaaaccggaa acgcgtcggt ggtttccacg gtcaagggcg
     6541 ccctgttcgg caacatcatc ttcggcggcg gcatcggcgc gatcatcgat cacgcgtccg
     6601 gctcggctta cgagtacccc cagctgatcc cgattgaaat gggatcggtg cgcgtcatcc
     6661 aggcgccgcg ggagccggaa acgccgcagc aaccgtgatc gccggcggag ccggatcgct
     6721 tcgcctgccc ttgcggcggg aggaagcgtt ccggaatcgg agtgccaggc tgaatctccc
     6781 ttgcatcatc atgcagagac cctcgactat caataacggc agcaagacga ccgagagttt
     6841 caacgaaccc ggggtaggtc aatttccttg cgcagcggac acgctaatgc cccgcgcaca
     6901 gcagcaggct ccacctcgtg ctaacatcgt ttcagtttcc cggacaggga attacacaag
     6961 tggaaacgtc cgtcgcaagg caggtaggag gggccggcgg cgccgcgatc agcgccgatg
     7021 cgctgattga aaccctgcgg cagctcgccg cggaactgca tccttcccgc gcgtccatcc
     7081 cctcctcgct cgacgcccgc ctcgagcagg actacggttt cgacagcctc gggcgcgtgg
     7141 agctgttcct gcgcatcgag cggcgcttcg gaacgagcct gaccgaaacc gtcatggcga
     7201 gcgcggaaac gccgcgcgat ctcctgcgtg cgatgctcgc ggcgagcccg caccaggtgc
     7261 atcccgccgc gctcgagcgc acgaccgcgc tggcgtcgga atcgcagaca ccggacgacg
     7321 ccgcgacgct gcctgaagtg ctcgaatggc acgttcgcaa gcatccctcc cgcccgcaca
     7381 tcgtgctgca ggacgacgaa gagcgcgagc gcgtcgtcac ctatgccgag ctcgaccagg
     7441 ccgcgcgcgc cgttgccgcc ggcttgatcg agcgcgggct cgagccgggc aaagcggtgg
     7501 cgatcatgct gccgaccggc gtcgagtact tctacagctt cttcggcatc ctgctcgcag
     7561 gcggcatccc ggtcccgatc tacccgcccg cgcgcccctc gcagatcgag gaccacctgc
     7621 ggcgccatgc ggggatactc tccaacgccc tcgcggcagc gctcatcacc gtgccgcagg
     7681 cgaagccgct ggcactgctc ctcaagccac gcgtggattc gctgcgcgaa gtggtgacgc
     7741 cggaggagct gatgc
//
LOCUS       DBNGSG010000063         7420 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2396237, whole genome shotgun sequence.
ACCESSION   DBNGSG010000063 DBNGSG010000000
VERSION     DBNGSG010000063.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7420)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7420)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7420
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2396237"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="AC158Q_01490"
     CDS             <1..237
                     /locus_tag="AC158Q_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HIR3338472.1"
                     /translation="WLVPHQANIRIIHASAKKLGMSMEKVVVTVDRHANTSAASIPLA
                     LDEAVRDGRIRSGQHILMEGVGGGFTWGAVLVKW"
     gene            275..1207
                     /gene="fabD"
                     /locus_tag="AC158Q_01495"
     CDS             275..1207
                     /gene="fabD"
                     /locus_tag="AC158Q_01495"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HIR3338473.1"
                     /translation="MKFAFVFPGQGSQSVGMMQPFAESKAARGVFAEASDVLDHDLWK
                     LVAEGPAEELNSTVNTQPVMLTAGYAAYRAWRDAGGAEPAMVAGHSLGEYTALVVGGA
                     LSFRDALPLVRFRAQAMQAAVPFGTGAMAAILGLEDDTVQAACGEAAQGQVVEPVNFN
                     APSQVVIAGHKAAVERGATAAKAKGARRAVMLPVSAPFHSSLLKPAADKLSDYLGKIN
                     VVAPRIPVINNVDVAMESDPQRIKDALARQACKPVRWVEIVRCMAGAGITHVVECGPG
                     KVLAGLTRRIDGNLQGLSITDPQSLGHALETLKS"
     gene            1204..1947
                     /gene="fabG"
                     /locus_tag="AC158Q_01500"
     CDS             1204..1947
                     /gene="fabG"
                     /locus_tag="AC158Q_01500"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HIR3338474.1"
                     /translation="MSFTGKVALVTGTSRGIGQAIALELGRRGAVIAGTSTTQAGADS
                     IQQNFSAAGIKGAGIVLNVNDAQQTEAAVAGIQKRFGDIAILVNNAGVTRDNLLVRMK
                     DEEWDEIMATDLKSVYRLSKLLMRSMMKARYGRIINITSVVGVTGNAGQTNYAAAKAG
                     IIGFSKSLAREVGSRNITVNCIAPGFIDTDMTRALSEQQREALVGQIPLGRLGRPEDV
                     AAAVGFLASAGADYMTGCTLHVNGGMYME"
     gene            2094..2333
                     /gene="acpP"
                     /locus_tag="AC158Q_01505"
     CDS             2094..2333
                     /gene="acpP"
                     /locus_tag="AC158Q_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR3338475.1"
                     /translation="MENIEQRVKKIVAEQLGVNEADVKNESSFVNDLGADSLDTVELV
                     MALEEEFETEIPDEEAEKIATVQQAIDYIKSHSKS"
     gene            2358..3596
                     /gene="fabF"
                     /locus_tag="AC158Q_01510"
     CDS             2358..3596
                     /gene="fabF"
                     /locus_tag="AC158Q_01510"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009913173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HIR3338476.1"
                     /translation="MSRRRVVVSGLGIVSPVGTGVPEAWKNILAGQSGITRITRFDPA
                     AFSSQIAGEVKGFDPAQYLSAKEARRFDTFIHYGLAGAIEAIRDSGLDFEREQREQVG
                     VCIGSGIGGLPLIEETHNAMLAGGPRKITPFFVPGSIINLISGQLSIMYGLKGPNLAV
                     VTACTTANHSIGEAGRLIEYGDADVMIAGGSEACVSPLGVGGFCAARALSGRNNDPAA
                     ASRPWDKDRDGFVLGEGAGILVLEEYEHARKRGAKIYCELAGYGMSADAHHITAPCED
                     GEGAVRCMSNALRNAGVNRDQVDYVNAHGTSTPLGDVAETVAVKSCFGNHAKKLAVSS
                     TKSMTGHLLGAAGGIEAVFSALAIRDQVAPPTINLVNQDPQCDLDFVPNTARKMKITV
                     ALSNSFGFGGTNGSLVFRKL"
     gene            3624..4436
                     /gene="pabC"
                     /locus_tag="AC158Q_01515"
     CDS             3624..4436
                     /gene="pabC"
                     /locus_tag="AC158Q_01515"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: protein motif:HMM:NF004761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HIR3338477.1"
                     /translation="MILVNGAAADSVAVTDRGLAYGDGVFRTLLSRGGRPKWWSWHYR
                     KLEHDCAALAITCPPEAALAADVALAASPSGDEVVKIMVTRGSGARGYAPPQPSSSTR
                     IVMTAPPPEYPDEFSRSGIRVHWCRTRLSSQPQLAGIKHLNRLENVMARAEWSDPAIA
                     EGLMLDQGGNVIGGTMTNLFIVEGDRMLTPELTQCGVAGVTRELVLQAARSRGVSCEE
                     AHITQERLLEAREAFLVNSVIGVWQIRECAGRSWTAGAHCGRVREWFDVEGH"
     gene            4420..5433
                     /gene="mltG"
                     /locus_tag="AC158Q_01520"
     CDS             4420..5433
                     /gene="mltG"
                     /locus_tag="AC158Q_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003264681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HIR3338478.1"
                     /translation="MSKVIERVLRVLFTTLLAGVVIFCGAFAYYATNPLEIETLPLQF
                     SLKAGSGLRSSAQQMARAGVLKHPERFVAMARLLGEASHIKPGIYEIDQPLTPYELLR
                     KITEGDLTQHAITFVEGWTFRQMRRALDEHPHVRHDSSGLEDVEILRRLKAQEASPEG
                     LFFPDTYHFNSGSSDLLVLRRAYRLMRSHLDAQWANRTPGLPLATPYEALILASIVEK
                     ETGKPVDRPLVAAVFVNRLRKGMLLQADPTVIYGMGQNFDGNIRKRDLTTDTPYNTYT
                     RTGLPPTPIAMPGLASLIATLHPPASDVIYFVSKGDGTSHFSRTLGEHERAVTKYQRS
                     GRR"
     gene            5430..6047
                     /gene="tmk"
                     /locus_tag="AC158Q_01525"
     CDS             5430..6047
                     /gene="tmk"
                     /locus_tag="AC158Q_01525"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012213208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HIR3338479.1"
                     /translation="MVRGKFITLEGIDGAGKSTHLRWIARFLRRNGVKVKLTREPGGT
                     AAGEKLRRLLLGGREPLHPETETLLVFAARREHLDKVILPALRAGQWVLCDRFTDATY
                     AYQSAGSGVDWAKVGVLERWVQGALQPDLTLLFDVSPDVGRRRAGRRKGSDRFEREKG
                     AYYQRVRKAYLKRAKGSPRRVRVINANRTMDDIHKQLEKLLSTNW"
     gene            6050..7069
                     /gene="holB"
                     /locus_tag="AC158Q_01530"
     CDS             6050..7069
                     /gene="holB"
                     /locus_tag="AC158Q_01530"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HIR3338480.1"
                     /translation="MVYPWHKSEFVSLLKRRNQLPHALLLRGPCGIGKLAYAEALAHA
                     LLCENPAPDGSACAQCTACGWMGQGSHPDFRRLEPEVLAGQKEADQQGGEKKEKAGTQ
                     IPVEQVRGIADFINISSHRGGVKLVLIHPAESLNASAANALLKNLEEPPPRTCFLLVA
                     HRWHQLLPTIKSRCQQIVLNPPSADEACGWLRQQGAGYPELALAQAGGAPLLAVGFDE
                     EYWQQRAWFLQAISSKGLDALAVAGELRDHAPAVVVNWMQKWSYDLVCHKVTGKVRYN
                     PDFATPISVAADRIELLEAVRFLRRVVRLQRIVLHPLNTRLFFEELLLIYAALLRDRP
                     LGKAA"
     gene            7091..>7420
                     /locus_tag="AC158Q_01535"
     CDS             7091..>7420
                     /locus_tag="AC158Q_01535"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR3338481.1"
                     /translation="MLVDSHCHLNFPELAAKVDDALALMRENGVTHALCVSVTLEEFP
                     QVRALTERYPHIFGSVGVHPDYPDVPAVGEAELIALARHPKIVAIGETGLDYFRIKGD
                     CEWQRERF"
BASE COUNT         1453 a         2219 c         2403 g         1345 t
ORIGIN      
        1 tggctggttc cgcatcaagc caacatccgc atcatccatg cctcggccaa gaaactgggc
       61 atgagcatgg aaaaagtggt cgttacggtg gatcgccacg cgaatacttc ggccgcctcg
      121 attccgctag ccctggacga ggcggtgcgg gacgggcgca tccggtccgg ccagcacata
      181 ctgatggaag gcgtcggcgg agggttcacg tggggtgcgg ttctggttaa atggtagttt
      241 ccggtcacca accgccaacc gcccgccgcg catcatgaag ttcgcttttg tctttccggg
      301 tcagggctcc cagtcggtcg ggatgatgca gcccttcgct gaatccaagg ccgcgcgcgg
      361 agtattcgcc gaagcttccg atgtgctcga ccatgacctg tggaaactgg ttgcggaagg
      421 gccggctgaa gagctcaatt ccaccgtcaa cacgcagcca gtgatgctca cggcgggcta
      481 tgcggcctac cgtgcctggc gcgatgcggg aggggcggag cccgcaatgg tcgcgggcca
      541 cagcctgggc gagtacaccg ccctggttgt tggcggcgcg ttgtcgttcc gcgacgcgct
      601 gccgctcgtg cgtttccgtg cccaggcgat gcaggcggcc gtgccgttcg gcaccggtgc
      661 aatggccgcg atcctcgggc tggaggacga tacggttcaa gccgcgtgcg gcgaggcggc
      721 acagggacag gtagtcgagc cggtcaactt caacgcgccc agccaggtcg taattgccgg
      781 gcacaaggca gcagtggagc gcggcgccac ggcggcaaag gccaaaggcg ccaggcgtgc
      841 tgtgatgctt ccggtcagcg cgccatttca ttcgtcgctg ctcaagccag cggcggataa
      901 attgtccgac tacctgggca aaattaatgt agtggcgccg cgcatcccgg tgatcaacaa
      961 cgtggacgtg gcgatggaaa gcgacccgca gcgcatcaag gatgccctgg cacgccaggc
     1021 atgcaaaccg gtgcgctggg tagaaattgt gcggtgcatg gccggcgccg ggatcactca
     1081 cgtcgtggaa tgcgggccgg gcaaggttct ggctgggctg accaggcgta tcgatggcaa
     1141 ccttcagggc ctgtcgatta ccgatccgca atccttgggc catgccctgg agacgctcaa
     1201 gtcatgagtt tcaccggaaa agtcgcactg gtcaccggga cctcacgcgg tatcggccag
     1261 gctattgccc tggaactggg caggcgaggc gccgtgatcg ccggcacgtc gactacccaa
     1321 gccggtgccg acagcatcca gcagaacttt tcggcggccg gcatcaaggg agccggcatc
     1381 gtactcaacg tcaacgatgc gcagcagacc gaagcggccg tggccgggat acaaaagcgg
     1441 ttcggcgata tcgcgatcct ggtcaataat gccggggtca cgcgcgacaa cctgctggtg
     1501 cgcatgaagg acgaggaatg ggacgagatc atggcaaccg atctcaagtc agtttaccgg
     1561 ctctccaagc tgctgatgcg ttccatgatg aaggcgcggt acggtcgaat catcaacatc
     1621 acctcggtgg tcggcgtcac tggaaacgcc ggccagacca actacgccgc ggccaaggct
     1681 ggcatcatcg gtttttcgaa atcgctggcg cgcgaggtcg gcagccgcaa tatcaccgtt
     1741 aattgcatcg cgccgggttt tatcgacacg gatatgacgc gggcattgag cgagcaacag
     1801 cgcgaagccc tggtcgggca aataccgctg ggccggctgg gccggccgga ggatgttgcg
     1861 gccgccgtgg gtttcctggc ttccgcggga gccgattaca tgaccgggtg cacgctgcac
     1921 gtcaacggcg gcatgtatat ggaataatga tgacagttat ctgaatttaa aagacttttt
     1981 tgatgagcga atctggtaaa atagcgccct gtttattacc aaacactctg gcctgaacgc
     2041 gtcgtcgggg tcggacacga gcattaacag cctttcgagg aaaggaaatc tgaatggaaa
     2101 atatcgagca acgcgtcaag aagattgttg ctgagcagct gggcgtcaac gaagcggacg
     2161 tcaagaacga gtcgtccttc gtcaacgacc ttggcgcgga ttcactcgac accgtggaac
     2221 tggtaatggc gctcgaggag gagttcgaga ccgagattcc cgacgaggaa gcggaaaaga
     2281 tcgccaccgt tcagcaggcc atcgactaca tcaagagcca ctcgaaatcc tgatctctcc
     2341 ccctgtccgg agcaaatttg tccaggcgca gagttgtcgt cagcggtctt ggtatcgtat
     2401 ccccggtcgg taccggggtg cctgaagcct ggaaaaacat actcgccggc caatccggca
     2461 ttacccgcat cacccgtttc gatccggctg ccttcagctc gcagatcgcg ggagaggtga
     2521 agggtttcga ccccgcgcag tatctgtccg ccaaggaggc gcgccgcttc gacacgttca
     2581 tccattacgg cctggcggga gcaatcgagg cgatcaggga ttcggggctc gatttcgagc
     2641 gcgagcagcg cgagcaggtc ggcgtgtgca tcggttccgg gatcggcggt ttgccgctga
     2701 tcgaggaaac gcacaacgcg atgctggcgg gcgggccgcg caagatcacg ccattcttcg
     2761 tgccgggctc gattatcaac ctgatatcgg ggcagctttc gatcatgtac gggctcaagg
     2821 gccctaacct ggcggtggtc acggcgtgca cgaccgccaa tcattccatc ggagaggcgg
     2881 gccgcctgat cgaatatggc gatgctgacg tgatgatcgc gggcggatcc gaagcgtgcg
     2941 tttcaccgct gggtgtcggc gggttctgcg ccgcccgcgc gctgtcgggg cgcaacaatg
     3001 accccgcggc agcgagccgg ccctgggaca aggaccgcga cggctttgtg ctgggcgagg
     3061 gcgccggaat actggtgctc gaagagtacg agcatgcaag aaagcggggc gcgaaaatct
     3121 actgcgaact ggccggctac ggaatgagcg cggacgcgca ccacatcacg gctccctgcg
     3181 aagacggcga gggggcagtg cgctgcatgt ccaatgcatt gagaaatgcc ggcgtcaacc
     3241 gcgatcaggt ggattacgtc aatgcccacg gcacttcgac gccgctgggc gatgtcgccg
     3301 agacggtggc agtcaagagt tgttttggca atcacgcgaa aaaactggcg gtaagctcga
     3361 ccaaatccat gaccggccac ctgctcgggg ccgccggcgg catcgaggcg gtcttctccg
     3421 cgcttgccat tcgcgaccag gtcgcgccgc cgacgataaa tctcgtcaac caggaccccc
     3481 aatgcgacct ggatttcgtg cccaacacgg cgcgcaagat gaagattacg gtagcccttt
     3541 cgaactcttt cggtttcggc ggcaccaacg gcagtctggt tttcaggaaa ctttgatacc
     3601 gcccgctctc cgggcgcatt tccatgatcc tggtgaatgg cgccgccgct gacagcgtcg
     3661 ctgtcaccga tcgcggactg gcttacggcg acggggtgtt tcgcaccttg ctgtcgaggg
     3721 gggggcggcc gaagtggtgg tcatggcatt accgcaagct tgagcacgat tgcgcagcgc
     3781 tcgcgattac ctgccctcca gaggcggcgc tcgccgcgga cgtggccctg gccgcaagcc
     3841 cgtccgggga cgaagtcgtc aagatcatgg tgactcgcgg cagcggtgcc cgcggttacg
     3901 cgcctccgca gccttcatcg tctacgcgca tcgtcatgac ggccccgccg ccggagtatc
     3961 cggatgagtt cagccggtcc gggatcaggg tccattggtg ccggacacgg ctgtcatccc
     4021 aaccgcagct tgccggcatc aagcatctca accggctgga gaacgtgatg gcccgcgccg
     4081 aatggagcga tccggccatt gccgagggtc tgatgctgga ccagggcggc aacgtcatcg
     4141 gcggcaccat gaccaatctg ttcatcgtcg agggcgaccg catgctgacg ccggaactca
     4201 cgcagtgcgg cgtcgcgggc gtgacgcgcg agctagtcct gcaggctgcc aggtcgcggg
     4261 gagtgagctg cgaggaagcg cacatcacac aggaacgcct gctggaggcg cgggaggctt
     4321 ttctggtcaa cagtgtcatc ggcgtatggc agatcaggga gtgcgccggc cgaagctgga
     4381 ctgccggggc tcattgcggg cgcgtgcgag aatggttcga tgtcgaaggt cattgaaaga
     4441 gtcttgcgcg tcctgttcac cacgctgcta gcgggcgtcg tgattttttg cggcgcattc
     4501 gcctactatg cgacgaaccc ccttgaaatc gagactttgc cgctccagtt cagcctcaag
     4561 gcggggagcg gattgcgcag ctcggcacag cagatggcgc gcgcaggcgt cctgaagcat
     4621 ccggagcgat tcgtcgccat ggcacgcctg ctcggagagg ccagccatat caagccgggc
     4681 atctacgaga ttgatcagcc gctcacgcca tacgagttgc tgcgcaagat caccgaaggc
     4741 gatttgacgc agcacgcgat caccttcgtc gagggctgga ccttcaggca gatgcgcaga
     4801 gcgctcgacg aacacccgca tgtgaggcac gacagtagtg gactggagga cgtcgaaatc
     4861 ctgcggcggt tgaaggccca ggaggcttcg ccagaagggt tattttttcc ggatacctac
     4921 catttcaaca gcggcagcag cgatctgctt gtcctgcgcc gcgcctaccg tttaatgcgc
     4981 tcgcatctcg atgcgcaatg ggccaatcgg acgccgggat tgccgctggc cacgccatac
     5041 gaagctctga tcctggcttc catcgtcgaa aaggaaaccg gcaaaccggt cgaccgcccg
     5101 ctggtagctg ccgtgttcgt gaaccggctg cgtaagggca tgctgttgca ggcggatccc
     5161 acggtgatct acgggatggg gcagaatttc gacggcaata tccgcaagcg cgatctcacg
     5221 acggatacgc cctacaacac ttacacccga accggcctcc cgccgacgcc catcgccatg
     5281 cccgggctgg cctcgctgat tgccaccctc catcccccgg ccagcgacgt gatttacttc
     5341 gtatcgaaag gggacggcac ttcccatttc tcccgcacgc tgggagaaca cgagcgcgcc
     5401 gtgacaaaat atcagcgatc gggacggcga tagtgcgcgg aaaattcatc acgctcgaag
     5461 gcatagatgg ggcgggcaaa agcacccacc tgcggtggat cgcccgcttc ctcaggcgta
     5521 acggcgtcaa ggtcaagctg acgcgcgagc ctggcgggac cgcggccggt gaaaaactgc
     5581 gccgattgct gcttggaggc cgggagccgt tgcacccgga aaccgagacg ctgctggtgt
     5641 ttgccgcacg gcgcgaacat ctggacaagg tcattcttcc ggctctcagg gccggccagt
     5701 gggtgctgtg cgaccgtttc accgatgcca cttacgccta tcagtcagcc ggcagcggtg
     5761 tggactgggc caaggtcggg gtgcttgaac ggtgggtgca gggcgccctg cagcccgatc
     5821 tgacactttt gttcgacgtc agcccggatg tcgggcgccg gcgcgccggc cggcgaaagg
     5881 gttctgaccg gttcgagcgc gaaaaaggcg cgtactacca gcgggtccgc aaggcctacc
     5941 tgaaacgggc caaaggctct ccccgccgtg ttcgcgtcat aaacgcaaat cgaaccatgg
     6001 acgatattca taaacaactt gagaaactgc tttcaactaa ttggtaataa tggtttatcc
     6061 atggcataag tcagagttcg tttcactgct gaaacgtcgg aaccagttgc cgcatgcgtt
     6121 actcctgcgt gggccctgcg gtatcggcaa gttggcgtat gcggaagccc ttgcgcatgc
     6181 gctgttgtgc gaaaacccgg cgccggatgg ttcggcctgc gcgcagtgca cggcctgcgg
     6241 atggatgggg cagggcagtc atcccgattt ccggcgcctc gagcccgaag tcctcgccgg
     6301 gcagaaagag gcggaccaac aaggcggcga gaagaaggaa aaggcgggca cccaaatccc
     6361 cgtggagcag gtcagaggca tcgcggattt catcaatatt tccagccatc gcggcggagt
     6421 gaaattggtg ctgatacacc ccgcggaaag cctcaatgcc agtgccgcca acgccctgct
     6481 gaaaaacctg gaagaaccgc cgccgcgcac ctgctttctg ctggtggcgc accgctggca
     6541 ccagctgctg ccgaccatca agagccgctg ccagcagatc gttctgaatc cgccgtctgc
     6601 cgacgaggca tgcggctggc tcaggcaaca gggggcgggt taccctgaac tcgctctggc
     6661 gcaggccggc ggggcgccgt tgctggcggt ggggttcgac gaggaatact ggcagcaacg
     6721 cgcttggttt ctgcaggcga tcagcagcaa ggggctggat gccctggcgg tcgccggcga
     6781 actgcgcgac catgcgccgg ccgtggtcgt gaactggatg cagaagtggt cttacgatct
     6841 ggtctgccac aaggtgacgg gaaaagtccg ctacaacccc gacttcgcca ctcccatttc
     6901 cgttgctgcc gatcgcatcg aactgttgga agccgtgcgc ttcctgcgcc gggtggtgag
     6961 attgcagcgc atcgtcttgc atccgctcaa cacgcgcctg ttcttcgagg agttgctgct
     7021 gatatatgcc gctctgctgc gggaccggcc gctcggcaag gccgcataac cgcgcgcatg
     7081 aggccggaac atgctggttg attcacactg ccatctcaat ttccccgaac tcgccgcgaa
     7141 agtggatgac gcgctggcgc tgatgcgcga gaacggagtc acgcacgcgc tgtgcgtcag
     7201 cgtcaccctg gaggaattcc cgcaggtgag ggcgctgacg gagcgttatc cgcacatctt
     7261 cggctcggtc ggcgttcacc cggactaccc ggacgttccg gcggtcgggg aagccgaact
     7321 gattgcgctg gcacgacacc ctaagatcgt ggccatcgga gaaaccggcc tcgattattt
     7381 ccggatcaag ggcgattgcg agtggcagcg cgagcgtttc
//
LOCUS       DBNGSG010000064         2774 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3245181, whole genome shotgun sequence.
ACCESSION   DBNGSG010000064 DBNGSG010000000
VERSION     DBNGSG010000064.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3245181"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="AC158Q_01540"
     CDS             <1..942
                     /locus_tag="AC158Q_01540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HIR3338482.1"
                     /translation="VFPEAMTGAADPRMTALVASEKAAFDARRQLIDGQAQLLRTQIN
                     EVREEIKGLESQIKFADDYIGYTREELEINERLLKDKFIAYTRILTLKRQLVEKEEKR
                     GEFVALVSQAKQKISERELRIITLYDNYVKEATEELRDVERRIADLEERLRPTEDQLK
                     RQTIIAPIAGEVVGLRVTTVGGVIAPREPLMDIVPIQPRVVVEGRVKVEDIDEVAVGQ
                     RVDVMLSAYKRRTTPKVAGKVTYVSGDSLADPGPNGAPYYLVHIEVDPKSLAEIGNLP
                     LMPGMPITAFIATRERTMLHYLLEPITDTLRRSLRES"
     gene            complement(961..2634)
                     /locus_tag="AC158Q_01545"
     CDS             complement(961..2634)
                     /locus_tag="AC158Q_01545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3338483.1"
                     /translation="MENSNAAIYFEPDGYVTSGERLMGRQAAGEGFLRGFVRHGGVRP
                     LYCCSESRAGAEVFARLCREHGAEEGAARWLRPDRLSALKEAGTLYLPGPALGDMAWR
                     RRRGDARAFSLCGVTHTIASHRAMDSLAGCQTAPVEPWDALVCTSRPVLESVRRLWDA
                     NADYLKQRFSARSVPEPALALIPLGVDCEALAPDPALGQQWRQRLGIGADDVAFLFLG
                     RLSFHAKANPYPMLVALERAAARVTARLHLIQAGWFPNDTIARQFREAARQLCPTVGA
                     IFVDGRKPDVRRGIWHAADVFTSLPDNIQETFGLTPVEAMAAGLPSVVSDWDGYRDTV
                     RDGLDGYRIPTLMPGPPAGEELAVRHEDGRDTYDRYIGYASQFVSVDIAGCAEAYTRL
                     ARDAALRKKLGAAARERARSEFDWSVIIRRYQDLWREQGRVRAAAGAAPARAAPANPL
                     RADPYWLFSSYATTVLDPACIVRLSDGASFERQQALRNSPLIGYARAVHPAEDLCRRL
                     IERLAQEGPCPVSGLVTKLAPAGFSELARAVAWLHKLGLLEVKAAGRAE"
BASE COUNT          460 a          979 c          968 g          367 t
ORIGIN      
        1 gtcttcccgg aggccatgac cggcgcggcc gacccgagga tgaccgctct cgttgcctcg
       61 gaaaaggccg cgttcgacgc gcggcgccag ctgatcgacg ggcaggcgca actgctgcgc
      121 acccagatca acgaggtgcg cgaggaaatc aaggggctcg agagccagat caagttcgcc
      181 gacgactaca tcggttacac ccgggaggag ctcgagatca acgagcgcct gctcaaggac
      241 aagttcatcg cctacacgcg gatcctcacg ctgaagcgcc agctggtgga gaaggaggag
      301 aagcgcggcg agttcgtggc gctggtctcc caggcgaaac agaagatcag cgagcgggag
      361 ctgcgcatca tcacgctcta cgacaactac gtgaaggagg cgaccgagga gctgcgggac
      421 gtcgagcggc ggatcgccga cctggaggag cgcctgcgcc cgaccgagga ccagctgaag
      481 cgccagacca tcatcgcgcc gatcgccggc gaggtggtcg ggctcagggt gaccaccgtc
      541 ggcggcgtca tcgccccgcg cgagccgctg atggacatcg tgccgatcca gccccgcgtc
      601 gtcgtcgagg gcagggtaaa agtggaagac atcgacgaag tggcggtcgg ccagcgcgtg
      661 gacgtgatgc tctccgccta caagcggcgc acgacgccga aggtggccgg gaaagtgacc
      721 tatgtttccg gcgacagcct ggccgatccc ggcccgaatg gcgcgcccta ctacctggtc
      781 cacatcgaag tggacccgaa gtcgctggcc gagatcggca acctgccgct gatgcccggc
      841 atgccgatca ccgcgttcat cgcgacgcgg gagcgcacga tgctgcacta cctgctggag
      901 ccgatcacgg acaccctgcg caggtcgctg cgcgagtcct gacgcgcggc gcgccccgcg
      961 ttactccgcc cgcccggcgg ctttcacttc gagcagcccg agcttgtgca gccaggcgac
     1021 ggcgcgcgcc agttcgctga acccggcggg cgccagtttc gtcaccaggc cggaaacggg
     1081 gcacggcccc tcctgcgcga gccgctcgat caggcgccgg cacaggtctt cggcgggatg
     1141 caccgccctg gcatagccga tcaagggtga attcctgagg gcctgctgcc gctcgaagct
     1201 ggcgccgtcg ctgaggcgca cgatgcacgc cgggtcgaga accgtcgtgg cgtaactgga
     1261 gaacagccag tagggatcgg cgcgcagcgg gttggcgggc gcggcccggg cgggcgcggc
     1321 gcccgcggcg gcgcgcaccc ggccctgctc gcgccacaag tcctggtagc gccggatgat
     1381 caccgaccag tcgaactcag agcgcgcccg ctcgcgcgcc gccgctccca gcttcttgcg
     1441 cagggccgcg tcccgcgcca ggcgcgtgta ggcctcggcg cacccggcga tatccaccga
     1501 tacgaactgg ctggcgtagc cgatatagcg gtcgtaggta tcgcggccgt cctcgtggcg
     1561 gacggcgagc tcctctcccg ccggcggccc cggcatcagg gtgggtatgc ggtagccgtc
     1621 gaggccgtcg cgcacggtgt cgcggtagcc gtcccagtcg cttaccaccg acggcagccc
     1681 cgccgccatc gcttccaccg gcgtcagccc gaacgtctcc tggatgttgt cggggagcga
     1741 agtgaacacg tcggccgcgt gccagatgcc gcggcgcacg tccggcttgc ggccgtccac
     1801 gaaaattgcg cccaccgtgg ggcagagctg ccgggccgcc tcgcggaact ggcgcgcgat
     1861 ggtgtcgttg gggaaccagc ccgcctggat gaggtgcagg cgcgcggtaa cccgcgccgc
     1921 ggcgcgctcc agcgccacga gcatcgggta cggattggcc ttggcatgaa agctcaggcg
     1981 ccccaggaac aggaacgcca cgtcatcggc gccgatgccc aggcgctggc gccactgctg
     2041 tcccagcgcg gggtcggggg cgagcgcctc gcagtccacg cccagcggga tcagcgcgag
     2101 cgcgggctcc ggcaccgacc gggccgagaa gcgctgcttc aggtagtccg cgttggcgtc
     2161 ccacagccgg cgcaccgatt ccagcaccgg ccgtgaagtg cagaccaggg cgtcccacgg
     2221 ctcgaccggc gctgtctggc acccggccag gctgtccatc gcgcggtgcg aggcgatggt
     2281 atgggtcacg ccgcagaggc tgaaggcgcg cgcgtcaccg cgccgcctgc gccacgccat
     2341 gtctcccaaa gcgggtccgg gcaggtagag tgtgcccgct tccttgagcg cggacaggcg
     2401 atccgggcgc agccagcgcg cggcgccctc ctccgctccg tgctcgcggc acaggcgcgc
     2461 gaacacctcc gcgccggcac gcgactcgga gcagcagtac agcggacgga cgccgccgtg
     2521 gcgcacgaag ccgcgcagga agccctcccc cgcggcctgg cggcccatca gccgctcgcc
     2581 cgaggtgacg tagccgtccg gctcgaaata aatcgccgcg ttggagtttt ccatcgacac
     2641 aatcgatccc tcgctgaatc acgcccgcgc catgataggc gcaatccggc gttcccggca
     2701 gccgcaaaaa aagcggccgc tcgaagcggc cgcctgaaac tcaacttccg cggcgccggc
     2761 gtttacgaca ggtg
//
LOCUS       DBNGSG010000065         3109 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1377596, whole genome shotgun sequence.
ACCESSION   DBNGSG010000065 DBNGSG010000000
VERSION     DBNGSG010000065.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1377596"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..1096)
                     /locus_tag="AC158Q_01550"
     CDS             complement(119..1096)
                     /locus_tag="AC158Q_01550"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3338484.1"
                     /translation="MPFQAPIVDFLSVRVVVDSRYERFLPKMNHPQVGIEHVGRIPGR
                     PDMTFACEWGLSLHLASEQNGAKAQYVLDFGYTPEIINRNFELLHIDPAKINGLILSH
                     GHRDHFGGLDGFVRHHRDRMRGDLALYVGGETIFRPKYVKEGGETVLWGAVDRDALAA
                     KQVASVCCPTPRALEGAFTSGYIERTSFEEVTGGSWVEDDHFSEAERKGKLVRDQHPD
                     EHATCYIVKGRGLVVISSCGHSGIVNTVKSSMAAANVDKVHAVIGGFHLGTSPQEYLQ
                     HSLDELKRINPDVILPMHCTGEAFIAMLKEKMPEKVVYSNVGSRFTFGG"
     gene            1829..2206
                     /locus_tag="AC158Q_01555"
     CDS             1829..2206
                     /locus_tag="AC158Q_01555"
                     /inference="COORDINATES: protein motif:HMM:NF024759.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HIR3338485.1"
                     /translation="MDFETIAREIVDSAIRVHTKIGPGMLESAYEACIEYELGKRSFA
                     VKKQVPMPLHYDDLTLDMGFRLDLVVEDAVVVELKSVKQLLPIHTAQLLSYLRAGDYR
                     LGFLLNFNTVHMRDGIKRVVNRL"
     gene            complement(2484..3038)
                     /locus_tag="AC158Q_01560"
     CDS             complement(2484..3038)
                     /locus_tag="AC158Q_01560"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HIR3338486.1"
                     /translation="MNLTLPEKSLFAILLRSRWWVSLLVALGTFGVVRLFLDAGYAFF
                     AAVPFIVIAVMALRRQRRVPSGARLDAAIDALRAMTWEEFARALEQGYRREGYVVKRV
                     EGAADFELERAGRLSLVAAKRWKASRTGVEPLKELAAAGEARGAAECVYVLAGETTRN
                     ALEYAKKTRINWLRGADLVKLAGG"
BASE COUNT          621 a          949 c          956 g          583 t
ORIGIN      
        1 ctgagggcgg gatgagggat gagggatgag ggatgaggga aattaaggca caagtcacaa
       61 ggcacaaggc acaaggcaaa agggtcgaga aacgagcaac gagtaacgag tcactccttc
      121 agccaccgaa ggtaaaccgg ctccccacat tcgagtaaac caccttctcc ggcatcttct
      181 ccttcagcat ggcgatgaac gcctcgccgg tgcagtgcat cggcaggatc acgtccgggt
      241 tgatgcgctt caactcgtcc aggctgtgct ggaggtattc ctgcggcgaa gtgccgaggt
      301 gaaagccgcc gatgacggcg tggaccttgt ccacgttcgc ggccgccatc gagcttttca
      361 cggtgttgac gatgccggag tggccgcagg aggagatcac caccaggccg cggcccttca
      421 cgatgtagca ggtggcgtgc tcgtccgggt gctggtcgcg cacgagcttg cccttcctct
      481 cggcttcgct gaaatggtcg tcctccaccc acgagccgcc ggtcacttcc tcgaacgagg
      541 tgcgctcgat gtagccggag gtgaacgcgc cctcgagcgc gcgcggcgtc gggcaacaga
      601 cggaggcgac ctgcttcgcg gcgagcgcgt cgcggtccac cgcgccccac aggaccgtct
      661 cgccgccctc cttcacgtac ttcgggcgga agatggtctc gccgccgacg tagagcgcga
      721 ggtcgccgcg catgcggtcg cggtgatggc gcacgaagcc gtccaggccg ccgaagtggt
      781 cgcggtggcc gtgtgagagg atcaggccgt tgatcttcgc cgggtcgatg tggagcagct
      841 cgaagttgcg attgatgatc tccggcgtgt agccgaagtc aagcacgtac tgcgccttcg
      901 cgccgttttg ctcggaggcc aggtgcagcg agaggcccca ctcgcaggcg aaggtcatgt
      961 ccgggcggcc ggggatgcgc ccgacgtgct cgattcccac ctgcgggtgg ttcatcttcg
     1021 ggaggaagcg ctcgtagcgc gagtccacca cgacgcggac ggagaggaaa tcgacgatcg
     1081 gagcctgaaa cggcatggaa acccccaagg tttaagggca cttaccaggg cattctacag
     1141 atgtgaggtt ccaatgtggc gccggcgtga caggtcgcgg ggagttcgta ctacggatcg
     1201 caaagagcgc aaaggatttg aagaaaagca cactacggat cacaaagggc gcaaaggacg
     1261 cgaggtagtg cgaactacgg atcgcgaagg aaatgataat aaggtcaaaa gcaaaacccg
     1321 aagggcgcgg aggaaacgcg ccaaacgtgg ccggcaagga ggctcctggt cgcattaccg
     1381 ctgcagaaat gtccctggtt ccaagtcgga tcgccaaggg cgcaggggac gcgaagtaga
     1441 acgcactacg gatcgcgaag gagatgataa taaggtcaaa agcaaaaccc gaagggcgcg
     1501 gaggaaacgc gccaagcgtg gccggcaagg aggcttctgg tcgcattacc tctgcaagtt
     1561 attcctgaat tccgtacccc gaccgagtat ccattaactc acttggcgtt gtcttcgcgt
     1621 ccttagtttt tcgtaccagt ttcctttgcg gcctttgcgt ccttcgtgac ccgtagtttg
     1681 tcttccttcg cgccctccgc gtccttagag acccgtcgtt aatgccttag cggtcttggc
     1741 gttccgtctt gagcccagcg cattccatgt caacccaaga ccatgacgcc cgttaacaga
     1801 tgaaatatgg gttgtgggga tattcttcat ggacttcgag acgattgcgc gggaaatcgt
     1861 tgattcggcg atccgggtgc atacgaagat cgggcccggc atgctcgagt ccgcctatga
     1921 agcctgcatc gaatacgagc tcggaaagcg ctcgtttgcc gtgaaaaaac aggtgcccat
     1981 gccgcttcat tacgacgacc tgaccctcga catggggttc cggctggacc tggtcgtcga
     2041 ggatgcggtc gtcgtggaac taaaatctgt caagcaattg ttgccaatcc atactgcaca
     2101 actgctgagt tatcttcgcg ccggcgacta ccggctgggc ttcctgctca acttcaatac
     2161 cgtccacatg cgcgacggta tcaagcgcgt cgtcaatcgg ttgtgactac cgcactgctg
     2221 agtaattgaa tcaggggcgc aaatgaatac caggcttggc gttgtcttcg cgcccttgct
     2281 gcggttttcg atatctgttt agcgacccgt agtggatacc ccgcgatggc gacccgtcgt
     2341 gaatggcatt taccctaagg cggaagggcc gggacacgag taacgagtaa cgagtaatga
     2401 aggcgggcag gcttctttga aaggctttcc tcttttgtgt cttttgtgtc ggggcttttt
     2461 cggttgtcgc gtatcgccgc ggctcatccc ccggccagct tcacgaggtc cgcgccccgc
     2521 agccaattga tccgcgtctt cttcgcatac tccagggcgt tccgggtcgt ctcgcccgcc
     2581 agcacgtaca cgcactccgc cgcgccccgc gcctcccccg cggccgccag ttccttcaag
     2641 ggctccactc ccgtgcgcga cgccttccac cgcttcgccg ccaccagcga caaccggccc
     2701 gcccgctcca gctcgaagtc ggctgcgcct tccacgcgct tcacgacata gccctcgcgc
     2761 cggtagccct gctccagcgc gcgggcgaac tcctcccacg tcatcgcgcg cagcgcatcg
     2821 atcgccgcgt cgagccgcgc gccgctcggc acgcggcgct gcctccgcag ggccatcacg
     2881 gcgatcacga tgaacggcac cgcggcaaag aaggcatagc ccgcgtccag gaacagccgc
     2941 accacgccga aggtgcccag cgcgacgagc aggctcaccc accaccgcga gcgcagcagg
     3001 atggcaaaca gcgacttctc gggcagggtc agattcattt atccggtgac gggtgacggg
     3061 aatcgcaaac ctggcgatga atgatgatgc gtctcaggtg aagagtccc
//
LOCUS       DBNGSG010000066         8047 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2566292, whole genome shotgun sequence.
ACCESSION   DBNGSG010000066 DBNGSG010000000
VERSION     DBNGSG010000066.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 8047)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8047)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8047
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2566292"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..93)
                     /locus_tag="AC158Q_01565"
                     /pseudo
     CDS             complement(25..93)
                     /locus_tag="AC158Q_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884072.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
     gene            complement(131..1204)
                     /gene="rfbB"
                     /locus_tag="AC158Q_01570"
     CDS             complement(131..1204)
                     /gene="rfbB"
                     /locus_tag="AC158Q_01570"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011402075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HIR3338487.1"
                     /translation="MSSSAILVTGGAGFIGANFVIDWLASEKTPVVNLDLLTYAGNTG
                     NLAGLKDDKQHVFVKGDINDRALVGRLLREHRPRAIVHFAAESHVDRSIHGPADFVQT
                     NVVGTFALLEEARNYWSSLKEPEKPLFRFLHVSTDEVYGSLGPDDSAFTETTPYAPNS
                     PYAASKAASDHLVRAYHHTYGLPTITTNCSNNYGPYQFPEKLIPLMVVNALARKPLPV
                     YGDGSNVRDWLYVGDHCEAVRAALARGRPGETYNIGGSAEKTNVEVVKAICAILDELR
                     PDDAPHERLIAFVKDRPGHDRRYAMDASRIHGELGWKPHERFDTGLRKTVRWYLDHMK
                     WVDGVTSGEYRKWIDLNYAHRAQ"
     gene            1315..2307
                     /gene="waaC"
                     /locus_tag="AC158Q_01575"
     CDS             1315..2307
                     /gene="waaC"
                     /locus_tag="AC158Q_01575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HIR3338488.1"
                     /translation="MPRILLVKTSSLGDVVHNLPVASDIRAAVPGAEIDWAVEESLAA
                     IPRLHPAVSRVLPVAVRRWRSGLLDGAVRAEVRAFIGELKRHPYDAVIDTQGLLKSAW
                     IAWIARGTRHGLDFASAREPLSFFYDRTYAVPWTVHAVERNRRLAAQALGYAVPASLD
                     YGVSAPPVSFVWLPGGRHAVLAHATSARSKLWPEGHWTEFGLRLDERGVRSVLPWGST
                     AERLRAEAIARAIPGAVVAPGLSLADAAALLAGADAVIGVDTGLTHLAAALKVPTIGI
                     YCATDPAATGLHGCVRAVNLGGIGAPPSVLDVLTALERLLPALSNHDSRFTNHE"
     gene            2304..3557
                     /gene="waaA"
                     /locus_tag="AC158Q_01580"
     CDS             2304..3557
                     /gene="waaA"
                     /locus_tag="AC158Q_01580"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HIR3338489.1"
                     /translation="MTRFFYTLLLYLLLPYVLARLVWRARRQRGYLEHVAERFGYYPR
                     DAGGPLIWVHAVSVGETHAAGPLIRALAARYPRHRVLLTHMTPTGRAAGQELFGEKVL
                     RCYLPYDYPGAIARFLHHFRPQAGILMETEIWPNLIHACRGEGIPLHLVNARLSEKSF
                     AGYRRFARLARESLAGLAAVAAQGADDARRLSALGASGVRVTGSVKFDVTPPPAQLDL
                     GRALRRDFGESRPVLLAASTREGEEALLLEVLEQANVPDLLTVIVPRHPQRFDEVGSL
                     LAARGIRFQRRSENTAIAPATRAVLGDTMGEMFAYYGACDVAFVGGSLLPLGGQNLIE
                     ACAVGRPVLIGPSTYNFAEAAELAVRAGAALQVPDAGALAREAGRLLRDPAAAQRMGQ
                     AGLAFCAAHRGATDKVLELITLRDQ"
     gene            complement(3610..4242)
                     /locus_tag="AC158Q_01585"
     CDS             complement(3610..4242)
                     /locus_tag="AC158Q_01585"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HIR3338490.1"
                     /translation="MTSKGHVDHSFREGPSITSKSEIRKQVLARRDALDAGERKTLSE
                     RITARLLALDAYRTAGCVMAYVSFGGEFETAGFIADLLARGRKLVLPRVERGGRALSL
                     RAVTDPGRELEAGVWGIRQPRADLCREVPAPEIAFVLVPGVAFTARCERLGYGGGFYD
                     RFIGGLAPRPALVAAAFGLQVVPELPTSKTDQRVDRVVTEDAEYGVTGDR"
     gene            complement(4264..5592)
                     /locus_tag="AC158Q_01590"
     CDS             complement(4264..5592)
                     /locus_tag="AC158Q_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HIR3338491.1"
                     /translation="MRAFLPISLALSLATATVANGADLLEIYRMAQSSDAQYAAARAT
                     WSAGQEKLPQGRAGLLPSASLSAGTQYNDRELQFRNTTNPPSNAQYNSNSASLSVTQP
                     LYRPQNMAAYEQSKAQVAQADAVFAQASQDLILRVAQAYFDVLLAQDTVELAQTQITA
                     IGQQLEQAKRNFEVGVATITDTHEAQSRYDLTVSLEITARNDLELRKRQLELLVGRAA
                     PGLAPLGKLFSTRPPEPNNSDRWVAIAGENNPLVHISRAGLEFAAREIERNRAGHRPT
                     LDAFATYSDSGTGSGIQGGSGNDTTSRVVGVQLAFPIYQGGLVNSRVREAVANEDRAR
                     QDLENARRTAELTARQTFLGVTNGISQVRALEAALVSSQSSLDSTRLGQEVGVRTQID
                     VLNAQQQLSQTRRDLAQAKYNYILALLRLKSAAGQLSEQDLAMVNAWLDR"
     gene            complement(5610..6263)
                     /locus_tag="AC158Q_01595"
     CDS             complement(5610..6263)
                     /locus_tag="AC158Q_01595"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase
                     family protein"
                     /protein_id="HIR3338492.1"
                     /translation="MNLEQARFNMVEQQIRTWEVLDQRVLDLLFRVHREDYVPQQYRA
                     LAFADMEIPIGHGETMLPPRMEARMLQELALRPGDKVLEVGTGSGYMTALFASLCGHV
                     YSVDLVAEFTRAAAAKLAAHGIANATLDTGDAARGWDRYEPYDAIALTGSVPVLPDAF
                     LKSLRPGGRLIAVVGEPPVMEAQLVTCVAHGAYSTTRLFETCIAPLRNALQPEKFVF"
     gene            6421..6795
                     /locus_tag="AC158Q_01600"
     CDS             6421..6795
                     /locus_tag="AC158Q_01600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR3338493.1"
                     /translation="MQLTNRRIGNAVVLAASGRIDHASADGFREALQPYLAQCKAGGD
                     VVVLDFSGVEYISSVGLRVLMLAAKQAKGQRGAVAVAALQPVVREIFEISKFTLVVPC
                     YAGVRDALTALAPAALAAYGAA"
     gene            6812..7771
                     /locus_tag="AC158Q_01605"
     CDS             6812..7771
                     /locus_tag="AC158Q_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3338494.1"
                     /translation="MLIRIWGSRGSLPVSLGAAALQDKLVKALVAAAGKGLDTEDKAR
                     AFIASGLDFPVAHTYGGNTSCVEIDNGAGEYVLCDLGTGAREFGNHVLDVHGHGKTHT
                     FHVFMSHVHWDHIMGFPFFAHAYLPGNRFRIYGCHDALESAFRRQHSAPSFPVEFDRL
                     AATVEFVQMKPGRTYDVAGMTVTAMRQAHSGDSYGYRFERGGKSVVYSTDSEHKLADP
                     RETEAFVTLFRDADLVIFDAMYSFAESVSVKEDWGHSSNIVGVELCQMANAKRLCLFH
                     HEPISDDERIAGIWRETRRLEEITRSGAPLEIIAAYDGLEIRV"
     gene            7779..>8047
                     /locus_tag="AC158Q_01610"
     CDS             7779..>8047
                     /locus_tag="AC158Q_01610"
                     /inference="COORDINATES: protein motif:HMM:NF017076.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HIR3338495.1"
                     /translation="MGAGPDSIARRWASYVIKRRGRPLGALILALLAVTLAVSGARVI
                     ERVRHVGFDAYQRLLPREREKAPAVIVEIDEKSLAQHGQWPWPRTL"
BASE COUNT         1198 a         2685 c         2755 g         1409 t
ORIGIN      
        1 gtcgtagacc gccagcagct cctacgatac cacctgggtc acgggataga gccgggtccc
       61 gctgccgccc gccaagagta tgccttttct cacgtcccga ctcgctgatt ggttgacgaa
      121 cgcgccccgc tcactgggcc cggtgagcgt agtttagatc aatccacttg cggtactcgc
      181 cgctggtgac tccgtccacc cacttcatgt gatcgaggta ccaccgcacc gttttcctga
      241 gcccggtgtc gaagcgctcg tgcggcttcc acccgagctc gccgtggatc cggctcgcgt
      301 ccatcgcgta gcggcggtcg tgtccgggcc gatccttgac gaaggcgatg agcctttcgt
      361 gcggcgcgtc atccggccgc aattcgtcca gtatcgcgca aatcgctttc acgacctcga
      421 cgttggtttt ttcggccgag ccgccgatgt tgtaggtctc gccggggcgg ccgcgcgcga
      481 gtgcggcccg cacggcctcg caatggtcgc cgacgtagag ccagtcgcgc acgttgcttc
      541 cgtcgccgta aaccggcagc ggcttgcggg cgagcgcgtt gacgaccatc agcggaatca
      601 gcttctcggg aaactggtac gggccgtagt tgttggagca gttggtcgtg atcgtcggca
      661 gcccgtaggt gtggtggtag gcgcgcacca ggtgatccga cgccgccttg gatgcagcgt
      721 acgggctgtt gggcgcgtag ggcgtggttt cggtaaatgc ggaatcgtcc gggccgagcg
      781 aaccatagac ttcgtcggtc gagacatgaa ggaaccggaa aagtggcttt tccggttcct
      841 tgagggaaga ccagtaattt ctcgcttctt cgagcagcgc gaacgtgccc accacgttgg
      901 tctgcacgaa gtcggcaggg ccgtggatcg agcggtccac gtggctttcc gcggcgaagt
      961 gcacgatcgc ccgcggccgg tgctcgcgca gcagccggcc gacgagcgcg cggtcgttga
     1021 tgtcgccctt gacgaaaaca tgctgcttgt cgtccttcaa gccggcgagg ttgccggtgt
     1081 tgccggcgta ggtcagcagg tcgaggttga cgacgggggt tttctcgctg gcgagccagt
     1141 cgatgacgaa attcgcgccg atgaagccgg ccccgccggt gacgagaatt gcgcttgaac
     1201 tcataggccg gccgcgcgcg cgagttggga tttttcccgt cggatgttaa cctgatgcac
     1261 cgtgcccccg acaatcaatt tttgccaatt ataacatata gatagcattg cttaatgcct
     1321 cggatactgc tggttaagac ttcctcgctg ggcgacgtcg ttcacaacct tcccgtggca
     1381 agcgacatcc gcgccgccgt gccgggcgcg gagatcgatt gggcggtgga ggagtcgctt
     1441 gccgccatac cgcggctgca cccggcggtc tcgcgcgtgc tgccggtcgc ggtccggcgc
     1501 tggcgctcgg gacttctgga cggcgccgtg cgcgcggaag ttcgcgcatt catcggcgag
     1561 ttgaagcgcc atccgtacga cgcggtgatc gatacccagg gactgctcaa gagcgcatgg
     1621 attgcctgga tcgcgcgtgg cacgcgccac gggctcgatt tcgcgagcgc gcgcgagccg
     1681 ctgtctttct tctatgaccg cacttacgcc gtgccgtgga cggtgcacgc ggtggagcgc
     1741 aaccggcggc tcgccgcgca ggcgctgggg tatgccgtgc cggcgagcct ggattacggc
     1801 gtgagcgcgc cgccggtaag cttcgtgtgg ctgcccggcg gacgtcatgc cgtgctggca
     1861 cacgcgacca gcgcgcgctc gaagctctgg cccgaggggc actggaccga gttcggcttg
     1921 cggctcgatg aacgcggcgt gcgcagcgtg ttgccctggg gcagcacggc cgagcggttg
     1981 cgcgcggaag cgattgcgcg ggcgatcccg ggggcggtgg tggcgccggg cctgtcgctt
     2041 gcggacgcag cggcgctgct cgcgggcgcg gacgcggtca tcggcgtgga caccggcctc
     2101 acgcatcttg ccgccgcgct caaggtcccg acgatcggca tctactgcgc gaccgacccg
     2161 gcggcgacgg ggctgcatgg ctgcgttcgc gccgtgaatc tcggcggcat cggggcgccg
     2221 ccttcggtgc tcgacgtgct cacggcgctc gaacggctgc tcccggcttt atcgaatcac
     2281 gactcacgat tcacgaatca cgaatgacgc gtttcttcta cacgctgctg ctctacctcc
     2341 tgctgccgta cgtgctcgcg cgcctcgtct ggcgcgcgcg ccgccagcgc ggctatctcg
     2401 agcacgtggc cgagcgattc gggtattacc cgcgggatgc cggcggaccg ctcatctggg
     2461 tgcacgcggt ctcggtgggc gaaacccacg cggccgggcc gctgatccgg gcgctggccg
     2521 cccgctatcc ccggcaccgg gtgctgctca cgcacatgac gccgacgggg cgcgccgcgg
     2581 gacaggagct gttcggcgaa aaagtcctcc gctgctacct gccatacgat tacccggggg
     2641 ccatcgcgag gtttctccat cattttcgtc cgcaggccgg catcctgatg gagaccgaga
     2701 tctggcccaa cctgattcac gcctgccgcg gcgagggcat cccgctgcac ctggtcaacg
     2761 cgcggctgtc cgagaaatcc tttgccggct accgccgctt tgcgcggctc gcgcgcgaaa
     2821 gcctcgcggg gctcgccgcg gtcgcggcgc aaggcgcgga cgacgcacgg cgcctgtctg
     2881 cgctcggcgc atcgggcgtg cgggttaccg gcagcgtcaa gttcgacgtc acgccgccgc
     2941 cggcgcagct cgatctgggc cgggccttgc gccgcgactt cggcgaatcg cgcccggtgc
     3001 tgctggccgc gagcacgcga gagggcgagg aggccctgct gctcgaggtg ctggagcagg
     3061 ccaatgtgcc ggacctgctg accgtcatcg tgccgcgcca tccccagcgc ttcgacgagg
     3121 tggggagcct gctcgcggcg cgcggcatcc gcttccagcg ccgcagcgaa aatacggcga
     3181 tcgcccccgc cacccgggcc gtgctcgggg acaccatggg tgagatgttc gcctactatg
     3241 gcgcgtgcga cgtcgcgttt gtcggcggca gcctgctgcc gctcggcggg cagaacctga
     3301 tcgaggcctg cgcggtgggc aggcccgtgc tgatcggacc ctccacgtac aatttcgcgg
     3361 aagccgccga gctcgcggtg cgggccggcg cggcgctgca ggtgcccgat gccggcgcgt
     3421 tggcgcggga ggccgggcgc ctgctgcgcg accccgcggc ggcgcagcgc atggggcagg
     3481 cgggactggc gttctgcgcg gcgcaccgcg gcgccactga caaggtcctg gagctgatta
     3541 cgctccggga ccaatgatga gtgctgagtg atgagtgacg agtaaagccg ttgatcgtcc
     3601 tgtcaccagt cacctatcac ctgtcacgcc gtattcagca tcctcggtaa cgacccgatc
     3661 gacccgctgg tcggttttcg acgtcggcag ctccggcacc acctgcagcc cgaacgccgc
     3721 ggcgaccagc gcgggacgag gggcaagacc gccaatgaac cggtcgtaga agccgccgcc
     3781 gtagccgagc cgctcgcaac gcgcggtaaa cgccacgccc ggcaccagca cgaacgctat
     3841 ttccggcgcg ggcacttccc ggcacagatc ggcgcgcggc tgccggatgc cccacacgcc
     3901 ggcctcgagt tcacgcccgg gatcggtcac cgcgcgcaga ctgagcgcgc ggccgccgcg
     3961 ttccacccgc ggcagcacca gtttcctgcc ccgcgcaaga agatcggcga tgaagcccgc
     4021 ggtctcgaac tcgccgccaa agcttacgta cgccatgacg cagccggccg tccggtaggc
     4081 gtcgagcgcg agcaggcgcg ccgtaatgcg ctcactcagg gttttgcgct cgccggcatc
     4141 cagcgcatcg cgccgcgcga gcacctgctt gcgaatttcg ctcttgctgg taatggacgg
     4201 gccttcgcga aatgagtgat cgacatgccc tttactcgtc acccgtcacc cgtcactcgt
     4261 cactcagcga tcgagccagg cgttgaccat cgcgagatcc tgctcggaga gctggcccgc
     4321 ggcggacttc agccgcagca acgcgaggat gtaattgtat ttcgcctgcg ccaggtcgcg
     4381 tcgcgtctgc gacagctgct gctgggcatt gagcacgtcg atctgcgtcc tcacgccgac
     4441 ctcctgcccg agccgcgtcg aatcgagcga actctgggag gacacgagcg cggcctccag
     4501 cgccctgacc tgggagatgc cgttggtgac gccgaggaaa gtctggcggg cggtgagctc
     4561 ggcggtgcgc cgggcgtttt ccaggtcctg tcgcgcccgg tcctcgttgg cgaccgcttc
     4621 gcgcacgcgc gagttcacga gcccgccctg gtaaatcggg aaggccagct gcaccccgac
     4681 caccctgctg gtggtgtcgt tgccgctgcc gccctggatg ccggagcccg tgcccgagtc
     4741 cgagtaggtg gcgaaggcgt cgagcgtggg ccggtgcccg gcgcgattgc gctcgatttc
     4801 gcgggccgcg aactccaggc ccgcgcggct gatgtgcacc agcggattgt tctcgcccgc
     4861 gatcgcgacc cagcggtccg agttgttggg ctcgggcggg cgcgtgctga acagcttgcc
     4921 gagcggggcg agcccgggcg ccgcccgccc gaccagcagc tccagctggc gctttctcaa
     4981 ctcgaggtcg ttgcgggcgg tgatctccag cgacacggtg aggtcgtagc gcgattgcgc
     5041 ctcatgagtg tcggtgatgg tcgcgacccc gacctcgaag ttgcgcttcg cctgctccag
     5101 ctgctggccg atcgcggtga tttgcgtctg ggcgagttcc accgtgtcct gggcgagcag
     5161 cacgtcgaag taagcctgcg ccaccctcag gatgaggtcc tgcgaagcct gcgcgaagac
     5221 cgcgtcggcc tgcgccacct gggccttcga ctgctcgtag gcggccatgt tctgcgggcg
     5281 atacaggggc tgcgtgaccg acagcgaggc ggagttggag ttgtactgcg cgttgcttgg
     5341 cggattggtc gtgttgcgga attgcagctc gcggtcattg tattgcgtgc cggccgacaa
     5401 ggaggcggag ggcagtagcc cggcgcggcc ttgcgggagc ttctcctggc cggcgctcca
     5461 cgtcgcgcgc gccgcggcat actgcgcatc ggagctttgc gccatgcggt agatctccag
     5521 caggtcggcg ccgttcgcaa cggttgccgt cgccagcgag agagcgagcg aaatcggtaa
     5581 aaatgcgcgc atcgccattt ccggcttttt caaaacacga acttctcggg ctgcagggcg
     5641 ttcctgagcg gcgcgatgca ggtttcgaac agccgcgtcg tgctgtacgc gccatgggca
     5701 acacaggtga cgagctgcgc ttccatcacg ggcggctcgc ccaccaccgc gatcagccgg
     5761 ccgccgggcc gcaggctttt caggaatgcg tccggcagca ccggaacgga gccggtgagg
     5821 gcgatcgcgt cgtagggctc gtagcggtcc cacccgcgcg cggcgtcccc cgtgtccagc
     5881 gtcgcatttg cgatgccgtg cgcggccagc ttggcggcgg ccgcccgcgt gaattcggcg
     5941 acgaggtcca cgctgtaaac gtgcccgcaa aggctggcga agagcgccgt catgtagccg
     6001 ctgccggtgc ccacttccag gaccttgtcg ccgggacgca gcgcgagctc ctgcagcatg
     6061 cgcgcttcca tcctgggggg gagcatggtt tcgccgtgtc cgatcggaat ctccatgtcg
     6121 gcgaacgcga gtgcgcggta ttgctgcggc acgtaatctt cgcgatgcac gcggaacaga
     6181 agatcgagca cccgctgatc caggacttcc catgtccgga tctgctgttc caccatgttg
     6241 aaacgcgcct gttcgagatt catggtttgc cccgcgcgga gcgttggatt ctaccatcct
     6301 ttgcagcgcc gacaggggcg ggaggcggta gaatcgcgtt gcccgggcgg actttttcca
     6361 gccgatgttc accgattgcc ccgcaccgtt tacactaccc gccagactgc gattgactcc
     6421 atgcaactca ccaaccggcg catcggcaat gccgtggttc ttgcggcgtc ggggcgcatc
     6481 gatcacgcga gcgccgatgg cttcagggaa gctctgcagc cctacctcgc gcagtgcaag
     6541 gcgggcggcg atgtcgtggt gctggatttt tccggggtcg agtacatcag cagcgtcggc
     6601 ttgcgggtgc tgatgctcgc cgccaagcag gccaaggggc aacggggcgc ggtcgcggtg
     6661 gccgcgctgc agccggtagt cagggaaatc ttcgagatca gcaagttcac cctggtggta
     6721 ccgtgttacg cgggggtgcg cgacgcgctc actgcgctcg ctcccgcggc gctcgccgcc
     6781 tacggcgcgg cgtgaccgga agccgccggc catgctgatc cgcatctggg gttcgcgcgg
     6841 ctcgctgccg gtttcgctcg gcgccgcggc gctccaggac aagctcgtca aagcgctggt
     6901 cgcggcggcg ggcaagggcc tcgacaccga ggacaaggcg cgcgccttca tcgcgagcgg
     6961 actcgacttc ccggtcgcgc acacctacgg cggcaacaca tcctgcgtcg agatcgacaa
     7021 cggcgccggc gaatacgtgc tctgcgacct gggaaccggc gcgcgcgaat tcggcaacca
     7081 cgtgctcgac gtgcacggcc acggcaagac gcacaccttc cacgtgttca tgtcccatgt
     7141 gcactgggac cacatcatgg gttttccctt tttcgcgcac gcctatcttc ccggcaaccg
     7201 cttccgaatc tacggctgcc acgatgcgct ggagagcgcg ttccgccggc agcattcggc
     7261 gccctccttc ccggtggagt tcgaccgcct cgcggcaacc gtcgagttcg tgcagatgaa
     7321 gcccggccgc acttacgacg tcgcgggcat gacggtgacc gcaatgcggc aggcgcactc
     7381 cggcgattcc tacggctacc gcttcgagcg cggcggcaag agcgtggtct actcgaccga
     7441 ttccgagcac aagctcgccg acccgcgcga gaccgaggcg ttcgttaccc tgttccgcga
     7501 cgcggacctg gtgattttcg acgccatgta ttcgttcgcc gagtcggttt cggtcaagga
     7561 ggactggggc cactcgagca atatcgtcgg cgtcgagctg tgccagatgg cgaacgcaaa
     7621 acgcctgtgc ctgttccacc acgagccgat ttcggacgac gagcgtatcg ccggcatctg
     7681 gcgcgagacg cggcggctgg aggaaataac ccgctccggc gcgccgctgg agatcatcgc
     7741 ggcctacgac ggcctcgaga tccgggtctg agcgcggcgt gggcgcggga ccggattcca
     7801 ttgcgcggcg ctgggcttcg tacgtcatca agcggcgcgg ccggcccctg ggggcgctca
     7861 tcctggccct gcttgcggtc acgcttgccg tctccggcgc gcgcgtcatc gagcgcgtgc
     7921 ggcacgtcgg gtttgacgcc taccagcgcc tgctgccgcg ggagcgcgag aaggcgcccg
     7981 cggtcatcgt cgagatcgac gagaaaagcc tcgcgcagca cggccagtgg ccgtggccgc
     8041 gcacgct
//
LOCUS       DBNGSG010000067        11443 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3925090, whole genome shotgun sequence.
ACCESSION   DBNGSG010000067 DBNGSG010000000
VERSION     DBNGSG010000067.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 11443)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11443)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11443
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3925090"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="AC158Q_01615"
     CDS             <1..242
                     /locus_tag="AC158Q_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3338496.1"
                     /translation="VAHLALRPGGRLVTYDNVYVEGQHWFARWLIGRDRGKAVRTRAQ
                     YEALACAAFPQVDSAILHDTLRVPYTILIMSCHRR"
     gene            complement(267..899)
                     /locus_tag="AC158Q_01620"
     CDS             complement(267..899)
                     /locus_tag="AC158Q_01620"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HIR3338497.1"
                     /translation="MASSVFVFGAGGHAKVVIDILERMKGVKVAFVAVDGESARGAKF
                     RGYPLIGGRDALLAARRGVKAGIVAIGDNAARARIAEWLAREGFRAASAIHPSAVIAR
                     GAEIGDGTAVMAGAVINTDARIGRHAIVNTGATIDHDCAIGDCTHVAPGCNLCGAVTV
                     GAGTLLGVGTRVIPRVRIGANAVVGAGSTVLTDIPDGARVAGSPCRPVES"
     gene            complement(889..1158)
                     /locus_tag="AC158Q_01625"
     CDS             complement(889..1158)
                     /locus_tag="AC158Q_01625"
                     /inference="COORDINATES: protein motif:HMM:NF014453.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR3338498.1"
                     /translation="MRSARIRHAQAGHVSIASSLLIMLSLSMLITALAVRLAMKPPVL
                     MRQERAGYRGEPFELTKLRTLTAAAGAQVHPLPDEVRLTRSERGQ"
     gene            1229..2701
                     /locus_tag="AC158Q_01630"
     CDS             1229..2701
                     /locus_tag="AC158Q_01630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR3338499.1"
                     /translation="MMEAGLEVVTIASFATLPLTMTAETTSSTGLTRRLWILYAAAVA
                     AFLPTLNFHYVGEEAIFPLVSLEMWHRGEWIRQFLFGQNLQHNPLFNWIIIVLCQLTG
                     WEHMLAVARAVTIAATASSGLVLAWLARRVTGDREFAAFAAIVYLMLADVVLYRGWLA
                     YVDPLFGFLVFASIACLWVACRERRLALLALAVAALTAGFLAKALTAYVFYGIAVLVL
                     ARQRDARRFLLSPASLAIHAAGAALLASWYGVLLSGEGQHSRMFQEIVDKLLPASLPG
                     YLWKLVGYPLETFLKLAPAGLLAAYYGWRRRDAADHAPQAFRIAALIALFNYLPYWLA
                     PQSHSRYLVPLYPFAALICAHLIWRARPVSIAVAVRWFYGVLAVKLVLAVALFPYYQA
                     VYRGANYARTAQEILERTAGHPLYTYNVTASGLSVKAHIDLARPGQPPLEFPPARWDS
                     GFVVAHAPDEKMGRVAAAFRLGGSNLYLLCRGAACREKRE"
     gene            complement(2775..3830)
                     /locus_tag="AC158Q_01635"
     CDS             complement(2775..3830)
                     /locus_tag="AC158Q_01635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012364359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HIR3338500.1"
                     /translation="MTRRIGDVLLATPLMRSVKRAWPEAALDALVFEGTQGVIEANPD
                     IRRVLTVPQKPGVFAHLAFLFRLARRYDVALSPLHGDRPTLYAFIAGRWRAGLVNDTR
                     RESWKKRLLNRWAPFDNLNTHTALMNLALAALAGIEPHRAVVVSWREEDAGRVAALPG
                     GAAAPPIAVLHPYPKFNYKMWHRAGWIETAGWLAARGHRIVLTGGADPAEIAYVADLA
                     RGMPPGTINLAGKLSLAETGCLLARAAVFIGPDTAPTHMAAALGVATVALYGPTDPVK
                     WGPWPGNPVPSAGPWRRCGSQRSGNVALLQGTGACVPCRLEGCERHVGSYSDCLQQLP
                     AARVIAAIEQLLDRPKA"
     gene            complement(3950..4714)
                     /locus_tag="AC158Q_01640"
     CDS             complement(3950..4714)
                     /locus_tag="AC158Q_01640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3338501.1"
                     /translation="MKLSVIVITRDNESTIRRCLESVAWADEIVVVDSGSADRTLEIC
                     REFTGKVHQPADWPGHGPQKNRALDLATGDWVFSLDSDEWITPEVRAEIEAAMAAPGN
                     RVAFMLPRRSSFCGRFMRHSGWWPDYVRRLFRRGAARFSDDRGHDRLIVNGAVGRLAS
                     PIHHETIVSLDQLIDKMNVYSAMTARNLHERGRGASILTALLHGGWAFFRTYFLRAGF
                     LDGREGLMLAIANAENSYYRYAKLALLGKKTVIGDR"
     gene            complement(4789..6006)
                     /locus_tag="AC158Q_01645"
     CDS             complement(4789..6006)
                     /locus_tag="AC158Q_01645"
                     /inference="COORDINATES: protein motif:HMM:NF016800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008754 - O-antigen ligase activity
                     [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR3338502.1"
                     /translation="MGAGIFAALTRTAQWAAVALGFSVPVSVALDNVLLAVVAACWLA
                     TGTWRESWRAARENAVALAALALYALLVLGTLYGEREPGDTVAYLAKYADLLFIPIFA
                     VLLRDPVLRRRALHALAISLVLVLAISYLIAAGMPALKPSLGSQANPVVFKQYLTHGI
                     LMAYGAFLFAEFALAEASRGRRALWAAAALAAAVNVIFMVQGRTGYLLLAVLAVYLGY
                     GWLRWRGLALAATATAAVFAALALVPGPFQQRLGLAGNVAAQTQAAQSARISNAERLE
                     LYRATGEIILDHPLLGVGTGGFPKAYAEKARNGNITASRNPHNEYLLIAAQLGLPGLA
                     ALLCLFWMHWRLAPRLASPLECHLARGLVLAIATGCLFNSLLLDHTEGLLYAWLTGVL
                     FAGLQSGGTENGK"
     gene            complement(5979..7799)
                     /locus_tag="AC158Q_01650"
     CDS             complement(5979..7799)
                     /locus_tag="AC158Q_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HIR3338503.1"
                     /translation="MNLNWRTLLAFAHDVTACAAAWLLAFWLRFNLDIPGYYLELALQ
                     TLPWVIAVHAGLFLGLGLYRGIWRYASLPDLRRIFVAVGVGAVAAPALLLMLQLQTPV
                     PRSVLIMSPILLMMSMGGSRIAYRAWKEGMPGWLLSSDREPVVVIGAQEAAVNLIKDL
                     ARSPQWIVVGVFDDDAAKIGRQLHGVNVLDRLDAIPQWRRRLGFAQAIIAMPEANHVV
                     RRRVVELCNRAGVKVLTVPSFEDLVSGRVTVSQVRHVELDDLLGRDPVALDAAGLRGW
                     LSGRVVMITGAGGSIGTELCRQVARFAPRKLVLFEQNEFALYTVEQEFTARHPQMAVV
                     AAAGDVKDAARAEQVIRAHAPSVIFHAAAYKHVPLMENENAWQAVRNNAFGTRVIARA
                     AIACGVEKFVLVSTDKAVNPTNVMGASKRLAEMICQSLHRPDGTHFIMVRFGNVLGST
                     GSVVPKFREQIARGGPVTVTHPEVTRYFMSIPEAAQLVLQAGLMGGKGEIFVLDMGEA
                     VRIADLARDLIKLSGFAEDDIRITYTGLRPGEKLYEEPLADGESLLPTPHPKLKVARA
                     RAENDGWLPRLESWLLQERVMDDAAVRAELAKWVPEYSPR"
     gene            complement(7864..8847)
                     /locus_tag="AC158Q_01655"
     CDS             complement(7864..8847)
                     /locus_tag="AC158Q_01655"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008342544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HIR3338504.1"
                     /translation="MQPASWAPLTAFAAALLIIWWLARGRLSTLILDHPTARSLHDSP
                     IPRTGGLGLHAGVLLAAGVISPNLPAALGIAFGVLLLISFLDDARSIPVLLRLAVHLA
                     ACALFAAAILLPGHGLLAVAIATLAIAWMANLYNFMDGSDGLAGGMAVIGFSCYGAAA
                     WLAGSTEFALVNFSIAAAAAAFLVFNSHPARIFLGDAGSVPLGFLAAALGLIGWLQGD
                     WTGWFAPLVFSPFIVDASVTLARRLARREKVWQAHRDHYYQRLVRLGWGHRATALAEY
                     CLMLACGGLALAGQALTAEMQAAALAAAALAYVVLIVLIERGWRRRVTGDG"
     gene            complement(8870..9826)
                     /locus_tag="AC158Q_01660"
     CDS             complement(8870..9826)
                     /locus_tag="AC158Q_01660"
                     /EC_number="5.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase family protein"
                     /protein_id="HIR3338505.1"
                     /translation="MAGDNVVLITGADGFVGRMLCEALAAAGRRVKRALRAVQPGLPD
                     AVAVGDIGGETDWRAALEAASAVVHLAARTHVLRETAADPLAEYRRVNVDGTRRLAQQ
                     AVRCGVRRLVFVSSIKVNGEATGRPFTENDAPRPGDAYGISKWEAEQALARVAAETGL
                     EVVVLRPSLVYGPGVKGNFLRLMNLVARRVPLPLASIANRRSLVYAGNLVDAVLKAID
                     APGAAGRTYLISDGEDVSTPDLVRALAQALGVAPRLLPCPPAALMIGATLAGRRAELA
                     RLAGSLQVDAAAARRDLGWRPRFTLSQGLAETARWYYSQSSA"
     gene            10070..>11443
                     /locus_tag="AC158Q_01665"
     CDS             10070..>11443
                     /locus_tag="AC158Q_01665"
                     /inference="COORDINATES: protein motif:HMM:NF024832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HIR3338506.1"
                     /translation="MQGTASVRHNIIANFAARAWSALMTLAFLPLYIRFLGIEAYGLV
                     GVYVSLVALFAILDMGLSTTLTRELARLSSAPDGGREARDLTRTFELIYWGVGILIGA
                     GVAALAPVIARHWLNVEGLPIETVEQSAMIMGLAMAMQWPISLYSGGLMGLQRQVLMN
                     IVRAGASTLQGAGAVLVLWLISPTILAFFSWQIAVSALQAAVIAACLWKSLPAADGSA
                     SFRGKLLAGNWRFAGGMTAIAVLVAVLTQSDKFILSKMLSLEMFGYYSLAAAIASALS
                     YISNPIFSALFPRLSQVVGRGNEAEVALIYHKGCQLMTATVVPVGTIVVLFSEELLTL
                     WMRDATTVGNIHLLVSLLVTGTVLNSLMILPYALQLAYGWTRLVLVVNAVSVVLFVPL
                     LILLADAYGGRGAALAWIALNAAYLVVVIPLMHRRLIRHSMGQWYLGDIGLPGGLCLG
                     IGALTYLV"
BASE COUNT         1869 a         3859 c         3892 g         1823 t
ORIGIN      
        1 aggtcgcgca tctcgcgctg cggccgggcg gacggctcgt cacctacgac aacgtctatg
       61 tcgagggcca gcactggttc gcgcgctggc tgatcggccg cgaccgcggc aaagcggtgc
      121 gcacccgcgc gcagtacgag gcgctcgcgt gcgccgcctt cccgcaggtg gacagtgcga
      181 tcctgcacga cacgctgcgc gtgccctaca ccatcctgat catgagctgc caccggcgat
      241 gagcgcgccg tcatccggcg ccggggtcat gattcgacag ggcggcacgg cgagccggcc
      301 acgcgcgcgc catccgggat atcggtgagg acggtcgagc ccgcgccgac caccgcattg
      361 gcgccgatgc ggacgcgggg aatgacgcgg gtgcccacac cgagcagcgt gcccgcgccc
      421 acggtgactg cgccgcacag attgcatccc ggcgcgacgt gcgtgcaatc gccgatcgcg
      481 cagtcatggt ctatcgtggc gccggtattc acgattgcgt gccgcccgat gcgggcatcg
      541 gtgttgatga cggcgcccgc cattaccgcc gtcccgtccc cgatctcggc gccgcgcgcg
      601 atgacggcgg acgggtggat ggcgctggcg gcgcgaaagc cctcgcgggc gagccactcc
      661 gcgatccggg cgcgcgcggc gttgtcgccg atcgcgacga tgcccgcctt gaccccgcgc
      721 cgggccgcca gcagcgcatc gcgcccgccg atgagcgggt agccgcggaa cttcgcgccg
      781 cgcgcgctct cgccgtcgac cgccacgaac gcgaccttca cgcctttcat gcgctcgagg
      841 atgtcgatca cgaccttggc gtggccgccc gcgccgaaca cgaaaacgct actggccacg
      901 ttcgctccgg gtgagccgca cttcgtccgg cagcggatgc acctgcgcgc cggcggcagc
      961 ggtcagggtg cggagcttcg tgagctcgaa cggctccccc cggtatcccg cgcgctcctg
     1021 gcgcatcagc accggcggtt tcatggcaag gcgcacggcg agcgcggtaa tgagcatcga
     1081 tagcgacaac atgatcagca gcgacgaggc gatagagaca tgacccgctt gagcatgccg
     1141 tattctagcc gatcgcattt gcgtacagga ctgctcacgc cccggtgagc gtgcgcggcg
     1201 ggaaactacg aaacgctcct gtgcggacgt gatggaggcg gggctcgagg tcgttacaat
     1261 agcgtccttc gcaaccctgc cgctgacgat gaccgcagaa acaacgtcct ccacgggcct
     1321 gacgcgcagg ctgtggatac tgtacgcggc ggcagtcgcg gccttcctgc cgacgctcaa
     1381 tttccattac gtcggcgagg aggcgatctt ccccctcgtt tcgctcgaaa tgtggcaccg
     1441 cggcgagtgg atccggcagt tcctgtttgg gcaaaacctg cagcacaacc cgttgttcaa
     1501 ctggatcatc atcgtgctct gccagctgac cggctgggag cacatgctgg cggtggcgcg
     1561 cgcggtcacc atcgccgcca ccgcgtcgtc gggcctggtg ctcgcatggc ttgcgcggcg
     1621 agtcaccggc gaccgcgaat tcgccgcctt tgccgcgatc gtctacctga tgctggcgga
     1681 cgtcgtgctc taccgcggct ggcttgccta tgtcgatccc ctgttcggct tccttgtgtt
     1741 cgcgtccatc gcctgcttgt gggtcgcctg ccgcgagcga cggctggcgc tgctcgcgct
     1801 cgccgtggcg gcgctcaccg cgggattcct cgccaaggcg ctcaccgcct acgtcttcta
     1861 cggcattgcc gtgctggtgc tcgcgcggca gcgcgatgcg cgccgcttcc tgttgagccc
     1921 ggcttcgctg gcaatccatg ccgcgggcgc tgcgctgctg gcgtcctggt acggcgtgct
     1981 gctcagcggc gaagggcagc acagccgcat gttccaggaa atcgtggaca agctgctgcc
     2041 ggccagcctt cccggctacc tgtggaagct cgtcggctat ccgctcgaaa ccttcctcaa
     2101 gctggcgccc gcggggctgc tggctgcgta ttacggctgg cggcgccggg atgccgcgga
     2161 ccacgcgccg caggcgttcc ggatcgccgc cctcatcgcg ctcttcaatt acctgccgta
     2221 ctggctggcg ccgcaaagtc acagccgcta cctggtgccg ctgtatccct ttgccgcgct
     2281 catctgcgcg cacctcatct ggcgcgcgcg cccggtgtcc atcgcggtgg ccgtgcgctg
     2341 gttctacggc gtgctcgcgg tcaagctggt ccttgccgtg gcattgtttc cctactacca
     2401 ggcggtctat cgcggcgcga actatgcccg gaccgcgcag gagatcctcg agcgcaccgc
     2461 gggccacccg ctctacacct acaacgtaac cgcgagcggg ctcagcgtga aagcccatat
     2521 cgacctcgcg cggcccggcc agccgccgct cgagttcccg cccgcgcggt gggacagcgg
     2581 tttcgtcgtc gcgcatgcgc cggacgaaaa aatggggcgt gttgccgccg ccttccggct
     2641 cggtggcagc aacctgtacc tgctgtgccg cggcgctgcc tgccgagaga agcgtgagta
     2701 gtgatgggtg aacagtgaat ggaaaggcgg cagcccgatt gggccccatg caaccgcgca
     2761 aatccaatca ccattcatgc ttttggccga tcgagtagct gctcgatcgc cgcgatgacg
     2821 cgcgcggcgg gcagttgctg caggcagtcg ctgtagctgc cgacgtggcg ctcgcagcct
     2881 tcgagacggc agggcacgca ggcgccggtt ccctgcagca gcgccacgtt gcccgagcgc
     2941 tggctgccgc agcgccgcca cggcccggcg gacggcacgg gattcccggg ccacggcccc
     3001 catttgaccg ggtcggtcgg cccgtagagc gcgaccgtcg cgacgccgag cgccgccgcc
     3061 atgtgggtgg gcgcggtgtc cggcccgatg aaaacggcgg cacgcgcaag caggcagccg
     3121 gtttcggcga gcgagagctt cccggcgaga ttgatcgtgc ccggcggcat gccgcgcgcg
     3181 agatccgcga cgtaggcaat ctccgcggga tcggctccgc cggtgagcac gatgcgatga
     3241 ccgcgcgccg cgagccagcc cgccgtctcg atccagcccg cgcgatgcca catcttgtag
     3301 ttgaatttgg ggtagggatg cagcacggcg attggcggcg cggccgcgcc accgggcagc
     3361 gcggcgacgc gtcccgcgtc ttcctcgcgc caggaaacga cgactgcgcg gtggggctcg
     3421 atgcctgcca gtgcggcgag cgccaggttc atgagcgcgg tgtgggtatt caggttgtcg
     3481 aacggcgccc agcggttgag gagccgcttc ttccaggact ccctgcgcgt gtcattcacc
     3541 agcccggcgc gccagcggcc cgcgatgaaa gcatagagcg tcggccggtc cccgtgcagc
     3601 ggcgagagcg cgacatcgta gcgccgcgcg agacgaaaaa ggaacgcgag gtgcgcgaac
     3661 acgcccggct tctgcggcac ggtgagcacg cgccggatgt cggggttggc ctcgatcacg
     3721 ccctgcgtgc cctcgaacac cagcgcatcg agcgcggcct ccggccaggc ccgtttgacc
     3781 gagcgcatca ggggcgtggc gagcagcacg tcgccgatgc ggcgcgtcac gatgataagg
     3841 acgcttcgcg gaggcgtcct catggggacg aaagggtgat aagtgataag tgataagtga
     3901 taagcagggc cggcaaagcc ggcatcgggg tcctgtcacc tatcacttat cacctatcac
     3961 ctatcacggt cttctttcca agcagcgcga gcttggcgta gcggtagtag gagttctccg
     4021 cgttggcgat ggcgagcatc aggccctcgc ggccgtcgag gaagcccgcg cgcaggaagt
     4081 aagtccggaa gaacgcccac ccgccgtgca acagcgcggt gagaatcgag gcgccgcggc
     4141 cgcgctcgtg caggttgcgc gcggtcatgg cggaatacac gttcatcttg tcgatcagct
     4201 ggtcgaggct cacgatggtc tcgtgatgga tggggctcgc aagccgcccg acggctccgt
     4261 tcacgatcag ccggtcatgt ccccggtcgt cggagaatcg cgccgcgccg cggcggaaca
     4321 ggcgcctgac gtaatccggc caccagccgg aatggcgcat gaagcgcccg cagaagctcg
     4381 agcgccgcgg cagcatgaac gccaccctgt tgccgggcgc ggccatcgcg gcctcgatct
     4441 cggcgcggac ttccggcgtg atccattcgt cggaatcgag ggaaaacacc cagtctccgg
     4501 tcgcgagatc gagagcgcga tttttttgcg ggccgtgccc gggccagtcc gcgggctggt
     4561 gaaccttgcc ggtgaattcg cggcagatct ccagggtgcg gtcggcgctg ccgctatcca
     4621 ccacgacgat ctcgtccgcc cacgccaccg attcgaggca gcggcgtatc gtggactcgt
     4681 tgtcgcgggt gatgacgatg accgagagct tcatggagcg cagtgaatgg tgaatagtga
     4741 atgatgaatg gtaacaacgg cgccgcgcgc gaggctgctt cccattgcct atttcccgtt
     4801 ttctgttccc ccggattgta gtccggcgaa gagcacgccc gtaagccagg catagagcag
     4861 tccctcggtg tggtcgagca gcagcgagtt gaacaggcac ccggttgcga tcgccagcac
     4921 gaggccgcgc gccaggtggc actcgagagg cgaagccagc cgcggcgcaa gccgccagtg
     4981 catccagaac aggcatagca gcgccgccag cccgggcagg ccgagctgcg ccgcgatgag
     5041 gagatattcg ttatgcggat tgcggctcgc ggtgatattg ccgttgcggg ctttttcggc
     5101 atacgccttc gggaagccgc cggtgccgac acccaggagc gggtgatcca ggatgatctc
     5161 gcccgtcgcg cgatacagct cgagcctttc ggcattcgag atgcgcgccg attgggccgc
     5221 ctgggtttgc gccgccacgt ttccggcgag tcccagccgc tgctggaacg gcccgggcac
     5281 cagcgccagc gccgcgaaca ccgcggccgt tgccgttgcc gcaagcgcca gcccgcgcca
     5341 gcgcagccag ccgtatccga ggtaaaccgc gagcaccgcc agcaacaggt aaccggtgcg
     5401 gccctgcacc atgaaaatga cgttgacggc ggcggcgagc gccgccgccg cccatagcgc
     5461 gcggcgtccg cgggaggctt ccgccagggc gaactccgcg aacaggaacg cgccgtacgc
     5521 catcaggata ccgtgcgtca ggtactgctt gaatacgacc gggttggcct gggagccaag
     5581 cgatggcttg agcgcaggca tgcctgccgc gatgaggtaa gagatcgcca ggaccagcac
     5641 gagcgaaatc gccagcgcgt ggagggcgcg ccggcgcagg acagggtcgc gcagcagcac
     5701 ggcgaatatg gggatgaaca gcaggtccgc gtacttggcc aggtaggcca cggtatcgcc
     5761 aggctcgcgc tcgccgtaga gcgtgcccag taccaggagc gcgtatagcg caagcgcggc
     5821 gagcgccacc gcgttttcgc gggccgcgcg ccacgattcc cgccaggtgc cggtcgccag
     5881 ccagcaggcg gccaccaccg ccagcagcac gttgtcgagc gcaaccgaaa ccgggacgga
     5941 aaaccccagc gccaccgcgg cccactgcgc ggtgcgggtc agcgcggcga atattccggc
     6001 acccatttgg ccagctcggc cctgacggcg gcatcgtcca tcacgcgctc ctgcagcaac
     6061 caggattcca ggcgcggcaa ccagccgtcg ttttccgcgc gggcccgggc caccttgagc
     6121 ttcgggtgcg gcgtgggcag caggctctcg ccatcggcga ggggctcctc gtagagtttt
     6181 tcgccggggc gcagcccggt ataggtgatg cggatatcgt cttcggcaaa accggagagc
     6241 ttgatgagat cgcgcgcgag gtcggcgatc ctgaccgcct cgcccatgtc gagcacgaat
     6301 atctcccctt ttccgcccat caatcccgcc tgcagcacga gctgggcggc ttcgggaatc
     6361 gacatgaagt aacgcgtgac ttcggggtgg gtgacggtga cggggccgcc gcgcgcgatc
     6421 tgctcgcgga acttgggcac cacgctgccg gtcgagccca gcacgttgcc gaagcgcacc
     6481 atgatgaaat gcgtgccatc ggggcggtgc agcgactggc agatcatttc ggcgagccgc
     6541 ttgctcgcgc ccatcacgtt cgtgggattg accgccttgt cggtcgagac cagcacgaat
     6601 ttctcgacgc cgcaggcgat cgcggcgcgg gcgatgacgc gggtgccgaa tgcgttgttc
     6661 cgcaccgcct gccacgcgtt ctcgttttcc atcagcggca cgtgcttgta ggccgcggcg
     6721 tgaaaaatca ccgagggggc gtgggcgcga atgacctgct cggcgcgggc ggcgtctttc
     6781 acgtcgcccg ccgcggccac caccgccatc tgcgggtgac gggcggtgaa ctcctgctcc
     6841 acggtgtaga gggcaaactc gttctgctcg aacagcacca gcttgcgcgg ggcgaagcgc
     6901 gcgacctggc ggcagagttc cgtgccgatc gagccgccgg cgccggtaat catcaccacg
     6961 cggccggaga gccagccgcg caggcccgcg gcgtcgagcg cgaccgggtc gcgcccgagc
     7021 aggtcgtcga gctccacgtg gcgcacctgc gagaccgtga cgcggccgct gaccaggtcc
     7081 tcgaacgagg gcaccgtcag caccttcacc ccggcgcggt tgcagagttc caccacccgg
     7141 cggcgcacga cgtggtttgc ctccggcatg gcgatgatcg cctgcgcgaa cccgagacgc
     7201 ctgcgccatt ggggtatggc gtcgagccgg tccagcacgt tcacgccgtg cagctgccgg
     7261 ccgatcttcg ccgcgtcatc gtcgaacacg cccacgacga tccactgcgg gctgcgcgcg
     7321 agatccttga tcaggttgac cgcggcttct tgcgcgccga tgacgacgac cggctcgcgg
     7381 tcgctcgaca acagccagcc cggcatccct tccttccagg cgcggtaagc gatccggctg
     7441 ccgcccatgc tcatcatgag caggatcggg ctcatgatca gcaccgagcg cggcacggga
     7501 gtctgcaact gcagcatgag gagcagcgcc ggcgcggcga ccgctcccac gcccaccgcg
     7561 acgaagatgc gcctcaaatc ggggagactg gcgtaacgcc agatgccgcg gtagagaccg
     7621 aggcccagga acaggcccgc gtgcaccgcg atcacccagg gcagggtttg cagggcgagc
     7681 tccaggtaat agccggggat gtcgaggttg aagcgcagcc agaacgcgag caaccaggcg
     7741 gccgcgcagg cggtcacgtc gtgagcgaaa gcgagcagcg tgcgccagtt gaggttcatc
     7801 ggccgccgcc cgtcaatggc aacccggtaa tgcagttgcc tcttgcttca agcccatcac
     7861 ccatcacccg tcacccgtca ctcgtctgcg ccagccgcgc tcgatcagga cgatcagcac
     7921 cacgtaggcg agagccgcgg ccgcgagcgc ggcggcctgc atctccgccg ttagcgcctg
     7981 tcccgccagc gcgagtccgc cgcacgcgag catcaggcag tactcggcga gggcggttgc
     8041 gcgatggccc cagcccaggc gcaccaggcg ctggtaataa tggtcgcggt gggcctgcca
     8101 cactttttcg cggcgagcga gccggcgcgc gagcgtgacg ctcgcgtcca cgatgaacgg
     8161 cgagaacacc agcggcgcga accacccggt ccagtcgccc tgcagccacc cgatcaatcc
     8221 cagcgcggcc gcgaggaagc cgagcggcac cgaccccgcg tcgcccagga agatgcgcgc
     8281 cggatgggaa ttgaatacca ggaaagccgc cgccgccgcc gcgatgctga aattcaccag
     8341 cgcgaactcg gtgctgccgg cgagccaggc cgccgcgccg tagcaggaga agccgatcac
     8401 cgccatgccg ccggcgagcc cgtccgaacc gtccatgaaa ttgtagagat tggccatcca
     8461 ggcgatggcg agagtggcga tcgcgacggc gagcagcccg tggccgggaa gcagaatggc
     8521 ggcagcgaag agcgcgcagg cggcaaggtg gacggcgagc cgcagcagta ccgggatgct
     8581 gcgcgcgtcg tcgaggaacg aaatcagcag cagcacgccg aaggcgatcc cgagcgcggc
     8641 cgggagattc ggcgagataa cgccggcagc gagcagcacc ccggcatgca gcccgagccc
     8701 cccggtgcgc ggtatcgggg aatcatgcag cgagcgggcg gtgggatgat cgaggatcag
     8761 agtggaaaga cggcccctgg cgagccacca gataatgagc aacgcggcag cgaacgccgt
     8821 cagcggggcc cacgaagcgg gttgcacaat ggcatcggcc tgagagtgct catgcactcg
     8881 actgcgaata ataccatcgg gcggtttcag ccagcccctg cgacagggtg aagcgcggac
     8941 gccatccgag atcgcgccgc gcggccgcag cgtccacctg cagcgagccg gcgaggcgcg
     9001 ccagttccgc gcgccggccg gccagcgttg cgccgatcat cagcgcggcg ggcgggcagg
     9061 gcagcaggcg cggcgcgacg ccgagcgctt gcgcgagcgc ccgcacgaga tcgggcgtgg
     9121 agacgtcctc gccgtcgctg atgagatagg tgcgccccgc ggcccccggt gcgtcgatcg
     9181 ccttgagcac tgcgtcgacg agattgccgg cgtagaccag gctgcggcgg ttggcgatcg
     9241 acgcgagcgg cagcggcacg cggcgcgcaa ccaagttcat caaccgcagg aagttgccct
     9301 tcacgcccgg cccgtagacg agcgacgggc gcaacaccac cacttcgagc ccggtttcgg
     9361 cggcgacgcg ggcgagcgcc tgttccgcct cccatttcga aataccgtag gcgtcaccgg
     9421 gccgcggggc atcgttctcg gtgaacggcc gcccggttgc ctcgccgttc accttgatgc
     9481 tgctcacgaa taccagccgg cgcacgccgc agcgcaccgc ctgctgcgcc agcctgcgcg
     9541 tgccatcgac attgacgcgc cggtattcgg ccagcggatc ggctgcggtc tcgcgcagca
     9601 cgtgggtgcg cgccgcgaga tgcaccacgg cgcttgcggc ttcgagcgcc gcgcgccagt
     9661 cggtctcgcc gccgatatcg cccacggcaa cggcatccgg gaggccgggc tgcacggcgc
     9721 gcagggcgcg cttcacccgg cggccggctg ccgcaagggc ttcgcacagc atccggccga
     9781 cgaatccatc cgccccggtt atcagcacga cgttgtcgcc ggccattcgt tacccgttac
     9841 cgcggacagt gctcaaacag ggaagcatgg gatggactgg taggcgttga gacggcgggt
     9901 aaactggctg cgctgactcc agtgacgtgg ggaagaatag cagatgtttt tcgcatgccg
     9961 tccgcccttc tcatggagag gcccggcgcc tgtgctagcc tctgcgtggt ccggaaaata
    10021 acctcgcttc cgatcgcgtc cgcccagttc gggaactacc gtaaccatca tgcaaggtac
    10081 cgcaagcgtc aggcacaaca tcatcgccaa ctttgcggca agagcctggt cggccctgat
    10141 gaccctggct tttctgcccc tgtatatccg gtttctgggt atcgaagctt atgggctggt
    10201 cggagtctat gtttcgctcg tggcgctgtt cgcgatactc gacatgggac tcagcaccac
    10261 gctcacccgg gaactcgcgc gcctgtcttc cgcgccggac ggggggcggg aagccaggga
    10321 tctgacgcgc acgttcgagc tcatctattg gggcgtcggc atcctgatcg gcgccggcgt
    10381 ggccgcgctg gcgccggtga tcgcgcgtca ctggctgaac gtcgagggcc ttcccattga
    10441 aacggtagag caatcggcca tgatcatggg actggccatg gcgatgcaat ggcccatctc
    10501 gctctacagc ggcggcctga tgggcctcca gcgccaggtc ttgatgaaca tcgtcagggc
    10561 gggagcgtcg acgctgcagg gcgcaggcgc ggtgctggtg ctctggctga tttctccaac
    10621 gatcctggcg ttcttttcct ggcaaatcgc cgtcagcgcg ttgcaggccg cggtcatcgc
    10681 ggcatgcctg tggaaatctt tgcccgcggc ggacggcagc gcctcgttcc gcgggaaatt
    10741 gctcgcgggc aactggcgct tcgccggcgg catgaccgcg atcgcggtac tggttgccgt
    10801 gttgacccaa tccgacaagt tcatattgag caagatgctc tcattggaaa tgttcggcta
    10861 ctattccctg gcggcggcca ttgcgagcgc gctgagctac atttccaatc ccatcttctc
    10921 cgccctgttt ccaagattgt cgcaggtggt tggccggggg aacgaggccg aagtagcgct
    10981 gatctatcac aagggctgcc agttgatgac ggcgacggtg gtgccggtcg ggacgattgt
    11041 cgtgctgttc tccgaggaac tgctgaccct ctggatgcgc gacgcgacca ccgtgggcaa
    11101 catccatctg ctggtcagcc tgcttgtcac cggcaccgtg ctcaattccc tgatgatcct
    11161 cccctacgcc ctccagctgg cctatgggtg gacgcggctg gtgctggttg tgaatgcggt
    11221 ctcggtggtg ctcttcgtgc ccttgctcat tctgctggca gatgcttacg gcgggcgggg
    11281 agccgcgctg gcgtggatcg cgctcaacgc cgcgtacctg gttgtcgtca taccgctcat
    11341 gcaccggcgc ctgatccggc acagcatggg gcagtggtac ctcggggaca tcggactgcc
    11401 gggcggcctt tgcctgggca tcggcgctct gacgtacctg gtc
//
LOCUS       DBNGSG010000068         4687 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3925786, whole genome shotgun sequence.
ACCESSION   DBNGSG010000068 DBNGSG010000000
VERSION     DBNGSG010000068.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4687)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3925786"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="AC158Q_01670"
     CDS             <1..437
                     /locus_tag="AC158Q_01670"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR3338507.1"
                     /translation="ERYDGKGYPRGLKGGEIGLFGGIAGLVDCFDALTHPRPYGETLA
                     PSNALNMLYGWRNAQFDGPLIEQFIHCVGIFPVGALVELYSSEIGIVIAQNPMMRLMP
                     RVMVVLDAKGKPVKPQKIVDRASIKRTLEKGSVPLDTSEFFL"
     gene            571..1797
                     /locus_tag="AC158Q_01675"
     CDS             571..1797
                     /locus_tag="AC158Q_01675"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HIR3338508.1"
                     /translation="MKKQVSVGELQFGVFIAELDRPWTETPFMFQGFVLKDDKQLEVL
                     KQHCKHVFIDSEKGLDLPDRKLQTGPLNTGPKGPSVLSSITNKTRYEELASVDAELPR
                     ARAAQGNTEVAVKDMLDSIRAGKAIDAPRVKEAVTSMTDRVVRNPDAMLLLAKMKEKG
                     ERNLDRAVGVSIYMITFGRFLQLPREQLDLLGMLGLLQDVGKMRVPEEVLNKTGPLTN
                     VEMALCRSHVEHSVAILKESPGLPPELPELAALHHERYGGSGYPKALKGPQIGLFGVI
                     AGLVDCFDALTHPRPFGEALAPSNALSMIYGWRDVQFDGPLVEQFIQCIGIFPVGAIV
                     ELNSGEMGIVIAQNLALRLQPRVMVVLDAKGNRKKPQVILDLAKDPRVNPDMPYRIKR
                     TLEKGSVPIDASEFFI"
     gene            1805..3571
                     /locus_tag="AC158Q_01680"
     CDS             1805..3571
                     /locus_tag="AC158Q_01680"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR3338509.1"
                     /translation="MDAPKAGSATAQEKETGVDLRALVGRLAEEFSADPKTLSRVASV
                     FTEVRDRAPDLFVPAAAALAPALESAAASGGSNVEPVYAALGEWALDAEAEPIGRMRG
                     DCMRFQAAMTRGALRLYAADPERCAETLVALQRLIFLQLALLVSLSAKTQGEGGVVRT
                     LPTPEYAAFMDIFRNTIESHKQEGKQLGLLLIHVSRVEQIDRLLGLQRGEAFMLRVTR
                     RMREGVLRKQDQLGRVSRDQLACLLPRIAGEGVAILAANKILAALETPIPIGDQSFAP
                     DAVIGIAVYPDHGADQQTLVRNGKLAVRAAIGTSERSAVYDASQGENEELKMRHEARL
                     RHALEQGTLDLAFEPQLDLKSGRIGGLECRLRWTDSELGKVPEAQAMETAEAGGLVRE
                     VTWWVFNNALRQSDEFSKAGLQIPISLKVTATSLFQPGFQEFVGRALRTWNVPPSRLV
                     IEIDESALTGPLEQVKETLVRLKAHGLRLGVDGFGAGASSLGNLAQLPLDEMKLATEF
                     TGDMKRAGARGKVVRSLIGLARDLGLRVVAEGVGDAETALALTTLGCDRIQGDYVSPA
                     LAAQEIVAFAAKLAGLSLRPQA"
     gene            3585..>4687
                     /locus_tag="AC158Q_01685"
     CDS             3585..>4687
                     /locus_tag="AC158Q_01685"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0140299 - molecular sensor activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3338510.1"
                     /translation="MQINFETIRGILERRLHQADIEKLLQGLSDSEKTQLFVRITELM
                     RRTTALVDIANRVSDTLSLDVLFPRLMDVVTESLNVERSSLFLYDPDTKELFSRVMQG
                     NAMGEIRFPADHGIAGSVFTSGEGVIIHDTYADMRFNRDVDTRTGYRTRNILCVPIRN
                     KKQEVIGVTQALNKRESQFDTDDQQLLEALSSQAAAALENARLFEKVDRAQREEAVLI
                     DVVSLIASEILLEPLLEKILTAATQLLGADRGSLFLYDPSRNELYSRVAGGVATREIR
                     FPANAGIAGECFSTGAPISIYDAYLDPRFNPEVDRGTGYRTRNILCMPITTKGGSKVG
                     VMEILNKKEGSFTTGDQRRLIALCAQAAVSIENA"
BASE COUNT          868 a         1473 c         1593 g          753 t
ORIGIN      
        1 acgagcgcta cgacggcaag ggctatcccc ggggcctcaa aggcggggaa atcgggctct
       61 tcggcggcat cgcgggtctc gtcgactgct tcgatgcgct gacccacccc cggccctacg
      121 gcgagacgct cgcgccctcg aacgcgctca acatgctcta cggctggcgc aacgcccagt
      181 tcgacggacc gctgatcgag cagttcatcc attgcgtggg catcttcccg gtgggcgccc
      241 tcgtcgagct gtattcgagc gagatcggga tcgtcatcgc gcagaacccg atgatgcggc
      301 tgatgccgcg ggtcatggtg gtgctcgatg cgaagggcaa accggtgaaa ccgcagaaga
      361 tcgtcgaccg ggcgagcatc aagcgcaccc tggaaaaggg cagcgttccg ctggatacct
      421 ccgaattctt cctctgaagg cggggacggc gccggccccc gattcgcgcg cccgggccgt
      481 gcgccgggcg cgcgcttgcg cgcagccccg gcaggacgca gaatcccggg ttcccgctgc
      541 cgggccagcc gcggcggggt tgacaactct atgaaaaagc aggtttctgt aggggagctg
      601 cagttcggcg tgtttatcgc cgagctagac cggccgtgga cggaaacgcc gttcatgttc
      661 caggggttcg tgctcaagga cgacaagcag ctcgaggtgc tgaagcagca ttgcaagcac
      721 gttttcatcg actcggagaa gggcctcgac ctgcccgacc gcaagctgca gaccggcccc
      781 ctcaacaccg ggcccaaggg cccgagcgtc ctctcgagca tcacgaacaa gacccgatac
      841 gaggaactgg caagcgtaga cgcggaattg ccgcgcgcgc gcgcagccca gggcaatacc
      901 gaagtcgcgg tgaaggacat gctcgactcg atcagggcgg gcaaggcgat cgatgccccc
      961 cgggtcaagg aagcggtgac gagcatgacc gaccgcgtcg tgcgcaatcc cgatgcgatg
     1021 ctgctgctcg cgaagatgaa ggaaaaaggc gagcgcaatc tcgaccgcgc ggtcggggtt
     1081 tccatctaca tgatcacgtt cggacgcttc ctgcagttgc cgcgggagca gctcgatctg
     1141 ctcgggatgc tcgggttgct gcaggacgtc ggcaagatgc gcgtgccgga agaggtgctc
     1201 aacaagacgg gaccgctgac caacgtcgaa atggcgttgt gcaggagcca cgtcgagcat
     1261 tccgtcgcga tcctgaagga gagccccggg cttccccccg agcttcccga actggccgcc
     1321 ctgcatcacg agcgctacgg cggcagcggc tatccgaagg cgctgaaggg cccgcagatc
     1381 gggttgttcg gggtcatcgc ggggctggtc gactgcttcg acgcgctcac gcacccgcgg
     1441 ccgtttggag aagccctcgc gccttcgaac gcgctcagca tgatctacgg ctggcgtgac
     1501 gtccagttcg acggcccgct ggtcgagcaa ttcatccagt gcatagggat ttttccggtc
     1561 ggcgccatcg tcgagctgaa ttcgggcgag atgggcatcg tgatcgcgca gaacctggcg
     1621 ctgcggctgc agccgcgcgt catggtagtg ctcgatgcca agggcaaccg gaaaaaaccg
     1681 caggtgatcc tcgatctggc aaaagacccg agggtgaatc cggacatgcc gtaccgcatc
     1741 aagcgcaccc tggaaaaggg cagcgttccg atcgacgcat ccgaattttt tatttgacga
     1801 ccgcatggac gcgcccaagg caggttccgc aaccgcgcag gaaaaggaga cgggcgtcga
     1861 cctgagggcg ctggtcgggc gcctggcgga ggaattcagc gccgacccga agacgctgtc
     1921 ccgggtggcg tcggtattca cggaagtgcg cgatcgcgcg cccgacctgt tcgttcccgc
     1981 tgccgcggcg ctcgctcccg cgctcgagag tgcggcggcc tcgggcggca gcaatgtcga
     2041 accggtctat gccgcgttgg gcgaatgggc gctggacgcc gaagccgagc cgatcgggcg
     2101 catgcgcggg gattgcatgc ggtttcaggc cgcgatgacg cgcggcgcgc tcagattgta
     2161 tgccgcggat ccggagcggt gcgcggagac cctggtggcg ctgcagcggc tcatctttct
     2221 gcaacttgcc ctgctcgtgt cgctttccgc aaaaacccag ggggagggcg gtgtcgtgcg
     2281 gacgctgccg acgccggaat acgcggcgtt catggatatc ttccgcaata cgatcgagag
     2341 ccacaagcag gagggcaagc agcttggcct gctcctcatc cacgtctccc gagtcgagca
     2401 gatcgaccgg ctgctcgggt tgcagagggg ggaagccttc atgctgcgcg tgacccggcg
     2461 catgcgcgag ggcgtgttgc gcaagcagga ccagctcggc cgggtgagcc gcgatcagct
     2521 cgcctgcctg ctgccgcgca tcgcgggcga gggcgtcgcg atactcgcgg cgaacaagat
     2581 actggccgcg ctggagacac cgatcccgat cggcgaccag tcgttcgcgc cggacgcggt
     2641 catcggcatc gcggtgtacc cggaccacgg cgccgaccag caaacgctgg tgcgcaacgg
     2701 caaactcgcg gtgcgcgcgg caatcggcac atccgaacgg tcagccgtgt acgacgcgtc
     2761 gcaaggcgag aacgaggaac tcaagatgcg gcacgaggcg cgcctgcgcc atgccctgga
     2821 gcagggcacg ctcgatcttg cgttcgagcc gcaactggac ctgaaatccg gccggatcgg
     2881 cgggctggaa tgccggctgc gctggacgga cagtgagctg ggcaaggttc ccgaagcgca
     2941 ggccatggaa acggccgagg ccggcggcct ggtgcgcgag gtcacctggt gggtattcaa
     3001 caatgcgctg cgccagagcg acgagttctc gaaagccggg ctgcagatac ccatcagcct
     3061 gaaggtgacc gcaaccagcc tgtttcaacc gggcttccag gaatttgtcg gacgcgccct
     3121 gcgcacctgg aacgttccgc ccagccggct ggtgatcgag atcgatgaga gcgcgctcac
     3181 cggaccgctc gaacaggtaa aggaaacgct cgtgcgcctg aaggcgcatg gattgcgcct
     3241 cggagtcgac ggtttcggcg ccggcgcgtc ctcccttggc aatctggcgc agttgccgct
     3301 cgacgagatg aagctcgcca cggaatttac cggcgacatg aagcgggcgg gcgcccgcgg
     3361 gaaagtcgtg cggtcgctga tcgggctggc gcgcgacctt gggctcaggg tcgtcgcgga
     3421 aggagtcggg gacgcagaga ccgcgctggc gttgacgacg ctcggctgcg accgcatcca
     3481 gggggattac gtgagccccg cgctggcggc ccaggaaatc gtcgcatttg ccgccaagct
     3541 ggccgggctt tcgctccggc cgcaggcctg aggcggtgcc ggccatgcaa atcaatttcg
     3601 aaaccatccg cgggatactg gagcgtcgcc tgcaccaggc ggacatcgaa aagctccttc
     3661 aggggctgtc cgattcggag aagacccagc tattcgtacg gatcaccgaa ctcatgcgcc
     3721 gcaccaccgc gctggtcgat atcgccaacc gggtgtcgga cacgctgtcg ctcgacgtgc
     3781 tgtttccccg cctgatggac gtcgtgaccg agtcgctgaa tgtggagcgc agttcgctgt
     3841 tcctgtacga ccccgacacg aaggaactgt tttcccgggt catgcagggc aacgccatgg
     3901 gggagatccg ctttcccgcc gatcacggga tagccggctc cgtgttcacc agcggcgagg
     3961 gggtgatcat ccacgatacc tatgccgaca tgcgttttaa ccgcgatgtc gacacccgca
     4021 ccgggtaccg gacgagaaac attctttgcg tgccgattcg caacaagaag caggaggtga
     4081 tcggggttac gcaggcgctc aacaagcgcg agagccaatt cgacacggac gaccagcaat
     4141 tgctggaagc gctgtcctcc caggccgccg ccgcgctcga gaacgcccgg ctgttcgaaa
     4201 aagtcgatcg cgcccagcgc gaggaggcgg tgctgatcga cgtggtgagc ctgatcgcct
     4261 cggagatact gctcgagccg ctgctggaga aaatcctgac ggcggccacc cagctgctcg
     4321 gcgcggaccg cggctccctc ttcctgtacg atccgtcgcg caacgagctc tactcgcggg
     4381 tcgcgggcgg cgtggcaacg cgcgagatcc gctttccggc caatgcgggc attgccggcg
     4441 agtgtttcag cacgggggcg cccatcagca tttacgatgc ctatctcgac ccgcggttca
     4501 accccgaggt ggacaggggt accggctacc gcacgcgcaa catcctgtgc atgccgatca
     4561 ccaccaaggg cgggagcaag gtgggggtga tggagattct caacaagaag gaggggtcgt
     4621 tcacgaccgg cgaccagagg cgcctgatcg cgttgtgcgc gcaggcagcg gtgtcgatcg
     4681 agaatgc
//
LOCUS       DBNGSG010000069         3819 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2228472, whole genome shotgun sequence.
ACCESSION   DBNGSG010000069 DBNGSG010000000
VERSION     DBNGSG010000069.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2228472"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..1369)
                     /locus_tag="AC158Q_01690"
     CDS             complement(149..1369)
                     /locus_tag="AC158Q_01690"
                     /inference="COORDINATES: protein motif:HMM:NF013740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR3338511.1"
                     /translation="MKAARSGAAAGTSRPGAAEPEFVSGMSAPVVDPVVVVAPAARYS
                     RVGVAVGVMMVIAFIAALYLARAFVVPLLIGLLASYALSPVVEWLKAVRIPRPAGAAL
                     VLAVLAGGSSWVAFSLSDDAAAMIEKLPEAARKLRQSLSAARASGPTALQNMQEAAKE
                     LEGATAEAAEASGKKKSPAARAIAARDAEPTAWLRDYMLAQSALVITVVAQTPIVLLL
                     AYFLLASGGHFRRKLVGLVGPSLTRKKDAVLILDEIDAQVQRYLLAMLVSNALVAVCT
                     WLAFEALGMEQAGIWGVAAGVLHFIPYLGPALIALASGVAAFLQFGSPILALAVAGVS
                     LLVAGTIGFGFMTWLHSRFASVNAAVLFIALLFFGWLWGIWGLLLGAPLIAIAKVICD
                     RVESLKPAGELLGR"
     gene            complement(1366..1812)
                     /locus_tag="AC158Q_01695"
     CDS             complement(1366..1812)
                     /locus_tag="AC158Q_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338512.1"
                     /translation="MKAQAGKSPYLPLLVTGIAIVLFSTGGIARMMGWIPDAIGYSGG
                     NLAPAQALPVPVADGIPATPRCPECGMIASVREIGTNGEDSGPAATGEAGADNGSGET
                     VTTTRNFEITVLMAGGSNRVLYLSNPANWRPGQRMILIDGANPPRR"
     gene            complement(1832..2155)
                     /locus_tag="AC158Q_01700"
     CDS             complement(1832..2155)
                     /locus_tag="AC158Q_01700"
                     /inference="COORDINATES: protein motif:HMM:NF017742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ElaB/YgaM/YqjD family protein"
                     /protein_id="HIR3338513.1"
                     /translation="MTNHTVDEFNRARSKMSGDIKTVITDTEDLLKAAAGVSGEGFAA
                     ARGKFGEKLESARARLADASRPAVDKARQSAAAVDGYVHGNPWTAIGVAAAAGLLIGF
                     LATRR"
     gene            complement(2304..2756)
                     /locus_tag="AC158Q_01705"
     CDS             complement(2304..2756)
                     /locus_tag="AC158Q_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338514.1"
                     /translation="MRRIGIAVLAVCAGLAGPAAAAGMELLSATRMVIAILADDLFVG
                     EAEGHLGGAGTVAIRSQQHPALTCLGQFTSSSVLGGSGEMRCSDGATATFDFKRLGVF
                     RGHGFGSTSRGPMSFVYGLTAREAGPYLKLPGGKRLTHNGTELELVDL"
     gene            complement(3001..3684)
                     /locus_tag="AC158Q_01710"
     CDS             complement(3001..3684)
                     /locus_tag="AC158Q_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3338515.1"
                     /translation="MAATRMGIVVSIAAALLPVLALAQDSKNQGYLVDTFGNNITTSG
                     TGLCWHTSDWTPARSVDPCDPAARKAEAPAPRVAAVSPPPRKPAPAPARMAPQKLNFS
                     ADALFDFDKAVLKPEGRAMLDDVARELNGALYEVIFVTGHTDRIGSHEYNRKLSERRA
                     YAVSDYLVSRDVAAHRVKAEGRGETQPVTRSGDCTGPVNSKLIACLQPDRRVHVEVTG
                     TKGVTARSR"
BASE COUNT          633 a         1311 c         1210 g          665 t
ORIGIN      
        1 attgcggtaa tgccgagttt gtgcattttc attccttgga gttgacggac catcgagttg
       61 atggaccatc ggtttcaggc gattgtggcc cccggccggg atccggtctg tgcgccggcg
      121 cgcttaaccg cctgcttatt gccggttatc agcgccccag cagctcgccg gcgggcttga
      181 gcgattcgac gcggtcgcag atcaccttgg cgatggcgat gagcggcgcg ccgagcagca
      241 gtccccagat gccccacagc cagccgaaga acagcaacgc gatgaacagc accgcggcgt
      301 tcacgctggc gaaccggcta tgcagccagg tcatgaaccc gaacccgatg gtgccggcca
      361 ccagcagcga cacgcccgcg accgcgagcg ccaggatggg cgagccgaat tgcaggaagg
      421 cggccacgcc gctcgccagc gcgatcagcg ccgggcccag gtaaggaatg aagtgcagca
      481 cgccggcggc gacgccccat atgcccgcct gctccatccc aagcgcctcg aacgcgagcc
      541 aggtgcatac cgccaccagc gcgttcgaca ccagcatggc gagcaggtag cgctgcacct
      601 gcgcgtcgat ttcatcgagg atcagcacgg cgtctttctt gcgggtcagc gacgggccga
      661 caaggcccac cagcttgcgc cggaaatgcc cgcccgacgc gagcaggaaa taggcgagca
      721 gcaacacgat cggcgtctgt gcgacgaccg tgatcaccag cgcggactgc gcgagcatgt
      781 agtcgcgcag ccaggcggtg ggttccgcgt cccgggccgc gatcgcgcgc gcggccggac
      841 ttttcttttt gccggaagcc tcggcggcct cggcagtggc cccttcgagc tccttggcgg
      901 cttcctgcat gttctgcagg gccgtcgggc cgctcgctcg cgcggcgctc aggctctgcc
      961 gcagcttgcg cgcggcctcc ggaagcttct cgatcatcgc cgctgcatcg tcgctcaacg
     1021 agaatgcgac ccaggacgag ccgccggcca gcacggccag caccagcgcc gcccccgcgg
     1081 gacgcgggat ccggaccgcc ttgagccact ccaccaccgg gctcagcgcg taactggcga
     1141 ggagcccgat cagcaacggc acgacgaaag cgcgcgccag atacagcgcg gcgataaacg
     1201 cgatgaccat cattacgccg accgccacac ccacgcgcga ataacgggcc gcgggcgcta
     1261 cgaccaccac cgggtccacg accggagcag acatccctga cacgaactcg ggctcggctg
     1321 cacccggacg cgaggtcccg gctgccgcgc ccgaccgcgc agctttcatc gtctcggcgg
     1381 attcgcgccg tcgatgagga tcatgcgttg cccgggccgc cagttcgccg ggttcgagag
     1441 gtagagcacg cgattcgatc cgcccgccat gagtacggtg atttcgaaat tccgggtggt
     1501 tgtcacggtt tccccgctcc cattgtcggc ccccgcctcg cccgttgccg cgggaccgga
     1561 gtcttcaccg ttcgtcccga tctcccgcac cgacgcgatc atgccgcatt cgggacacct
     1621 cggcgtggca ggaatcccgt ccgccaccgg cacgggtagc gcctgtgcgg gcgcaaggtt
     1681 gccgccggaa tagccgatgg catccgggat ccagcccatc atgcgcgcaa tcccgccggt
     1741 gctgaacagg acgatcgcga tcccggtgac cagcagcggc aggtacggag attttccggc
     1801 ttgcgctttc atgcgatggc tcgatccagt cttaacgccg ggtggccaga aagccgatca
     1861 acaggccggc ggcggcggcg acaccgatcg cggtccacgg attgccgtgc acgtagccgt
     1921 cgaccgcggc ggcactttgc ctggccttgt cgacggccgg ccgcgacgcg tccgccagtc
     1981 tcgcccgggc gctttccagc ttctcgccga atttcccgcg tgcggccgcg aaaccttctc
     2041 cggatactcc ggccgccgcc ttgagcagat cctcggtatc ggtgatgacc gtcttgatat
     2101 cgccggacat cttgctcctg gcccggttga attcatctac tgtgtgattc gtcattgctt
     2161 tctccttggg tttaactgtt tgccgttcat ggtttgccgc acctgttcac gtcctgaatc
     2221 cgacctgccg gttcttgtcc gcgagcaact ggatgagccc ctcgatgtgt cctctgatat
     2281 tgaatgtcat cggctgtctt ctcctacagg tcaaccagtt ccagctccgt cccgttgtgc
     2341 gtgagccgct tgcccccggg cagcttcagg tagggccccg cttcccgagc ggtcagcccg
     2401 tacacgaaac tcatcggtcc ccggctggtg ctgccgaaac cgtggccgcg gaacacgccc
     2461 aggcgcttga aatcaaatgt cgcggtggcg ccgtcactgc aacgcatctc ccccgaaccg
     2521 cccagcacgc tgctggaagt gaactggccg agacaggtga gtgcggggtg ctgctgcgag
     2581 cgaatggcaa cggttcccgc tccgccgaga tggccctcgg cctcgcccac gaacaggtca
     2641 tcggcgagga ttgcaatgac catgcgcgtc gccgacaaca gctccatgcc cgctgctgct
     2701 gccgggccgg ccagcccggc gcagacggca agaaccgcaa tccctattct gcgcattgaa
     2761 gtccgccccg atggaagcgc tcgacagctg aaaactgcgg ccgctaaggc cagttgtcat
     2821 tgcgcgagca tggcccctgc ctcgagggcg cgctgtgcgt tggcgtacgc aagaagaaag
     2881 aaatcatgcc aacgggcgcc agtcacgatg aagcaacccg caaagatcgt gacgggcctg
     2941 gagcccgccg atcgataccc ggaaagcagc cataccccgc tggcagcgga gcggcgctgc
     3001 ttatcgcgac ctcgccgtca cgcctttggt gccggtcact tcgacatgca cccggcgatc
     3061 gggctgcagg caggcgataa gcttgctgtt gaccggcccc gtgcaatcac cggaccgggt
     3121 gaccggctgc gtctcgcccc tgccttccgc cttgacgcga tgggccgcga catcccggct
     3181 caccaggtaa tccgacaccg catacgcgcg gcgctccgaa agcttgcggt tgtactcatg
     3241 gctgccgatg cggtcggtgt gtccggtcac gaagatcact tcgtacagcg cgccgttgag
     3301 ctcgcgcgcc acgtcgtcca gcatcgccct gccttcgggc ttcagtaccg ctttgtcgaa
     3361 atcgaacagc gcgtccgccg agaaattgag tttctgcggc gccatcctgg ctggcgccgg
     3421 cgcgggtttc cgcggcggcg gagagacggc cgcgacccgg ggtgcggggg cttcggcttt
     3481 tctggcggcg gggtcgcagg gatcgaccga gcgcgccggc gtccagtcgc tggtgtgcca
     3541 gcaaaggccc gtgccgctgg tcgtgatgtt gttcccgaac gtatccacca ggtaaccctg
     3601 gtttttggaa tcctgcgcga gcgccaggac tggcagcaac gctgcggcaa tgctgacgac
     3661 tatccccatt cttgttgcgg ccatgatcgt tttccttttg tcggcttcac ccggcgtgct
     3721 cggggcatcg ccaatcgcta cgccattgtc ccgtctcgtc ccgggatccg cagtgcgctg
     3781 gcgtacatag aggggggggg ggggggggcg aaattaagt
//
LOCUS       DBNGSG010000070         1517 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3757524, whole genome shotgun sequence.
ACCESSION   DBNGSG010000070 DBNGSG010000000
VERSION     DBNGSG010000070.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3757524"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..85
                     /locus_tag="AC158Q_01715"
     tRNA            1..85
                     /locus_tag="AC158Q_01715"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Leu,seq:gag)
     gene            126..494
                     /locus_tag="AC158Q_01720"
     CDS             126..494
                     /locus_tag="AC158Q_01720"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HIR3338516.1"
                     /translation="MLEEYFPILLFIFVGIAVGVGPLLAGGIVSRLVGVHRPDSEKLS
                     PYECGFEAFEDARMKFDVRYYLVAILFILFDLEIAFLFPWAVVLQEIGFFGFMSMMVF
                     LGILVVGFIYEWKKGALEWE"
     gene            523..1002
                     /locus_tag="AC158Q_01725"
     CDS             523..1002
                     /locus_tag="AC158Q_01725"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NuoB/complex I 20 kDa subunit family protein"
                     /protein_id="HIR3338517.1"
                     /translation="MGAIETITEKGFVTTTADALINWTRTGSLWPMTFGLACCAVEMM
                     HAGASRYDLDRFGIVFRPSPRQSDVMIVAGTLCNKMAPALRKVYDQMAEPRWVISMGS
                     CANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGIIQLQNKIKRTNTIAR"
     gene            1029..>1517
                     /locus_tag="AC158Q_01730"
     CDS             1029..>1517
                     /locus_tag="AC158Q_01730"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HIR3338518.1"
                     /translation="MTPRAETLIAALQSALGDRLVSVAATLGEVTAVVKPEHLLAAAA
                     TLRDAPGLKFEELIDVCGVDYRDYGDGAWEGRRFAAVYHLLSIALNQRLRVRVFCPDD
                     EFPVVDSVTGIWPSANWYEREAFDLYGIIFTGHADLRRILTDYGFIGHPFRKDFPLSG
                     HVE"
BASE COUNT          263 a          472 c          485 g          297 t
ORIGIN      
        1 gccgacgtgg tggaattggt agacacacca tcttgagggg gtggcggcgc aagccgtgag
       61 agttcgaatc tctccgtcgg caccatagtt gttaaggaac caggggaaca gagaccgagt
      121 tcgacatgct ggaagagtat ttccccatcc tgctgttcat tttcgtcggc atcgccgtcg
      181 gcgtggggcc gctgctggcg ggcggcatcg tcagccggct ggtcggcgtg caccgcccgg
      241 actccgaaaa gctctcgccc tacgagtgcg ggttcgaggc gttcgaggac gcgcgcatga
      301 aattcgacgt acgttactac ctggtcgcga tcctgttcat cctgttcgac ctcgaaatcg
      361 cgtttctctt cccctgggcc gtggtgttgc aagagatcgg ctttttcggc ttcatgtcga
      421 tgatggtatt cctggggata ctcgtggtcg gctttatcta cgagtggaag aagggcgcgc
      481 tggagtggga atgaaagtaa cggcaatcgc gaggtgacct tcatgggtgc gatagaaacc
      541 atcaccgaga aaggcttcgt caccaccacg gcggacgcgc tgatcaactg gacgcgcacc
      601 ggctcgctgt ggccgatgac cttcgggctc gcctgctgcg ccgtggagat gatgcacgcc
      661 ggcgcctcgc gctacgacct cgaccgcttc ggcatcgtgt tccggccgag cccgcgccag
      721 tccgacgtga tgatcgtcgc cggcacgctg tgcaacaaga tggcgccggc gctgcgcaag
      781 gtctacgacc agatggcgga gccgcgctgg gtgatctcga tgggttcctg cgccaacggc
      841 ggcggctatt accactattc ctactcggtc gtgcgcggct gcgaccgcat cgtgccggtg
      901 gacatctacg tgccgggctg cccgcccacg gccgaagccc tgctctacgg catcatccag
      961 ctgcagaaca agatcaagcg aacgaatacg atcgctcgat agaaatctcg ctcagccatc
     1021 gcacgctcat gaccccccgt gcagaaactt tgatcgccgc gctgcagtcc gcgctcggcg
     1081 acaggctcgt cagcgttgcc gccaccctgg gcgaagtaac tgccgtggtc aaaccggaac
     1141 acctgctggc cgccgcggcc acgctgcgcg acgcgccggg gctgaaattc gaggagctga
     1201 tcgacgtgtg cggcgtcgat taccgcgatt acggtgacgg ggcatgggag ggcaggcggt
     1261 tcgccgcggt ctatcacctg ctcagcatcg cgctcaacca gcgtcttcgc gtgcgggtgt
     1321 tctgcccgga tgacgagttc ccggtggtgg actcggtgac cggcatctgg ccttcggcca
     1381 actggtacga acgggaggcg ttcgatctct acggcatcat cttcaccggg cacgccgacc
     1441 tgcgccgcat cctgaccgat tacggcttca tcgggcaccc gttccgcaag gatttccccc
     1501 tgagcgggca tgtcgag
//
LOCUS       DBNGSG010000071        11285 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3758181, whole genome shotgun sequence.
ACCESSION   DBNGSG010000071 DBNGSG010000000
VERSION     DBNGSG010000071.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 11285)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11285)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11285
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3758181"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..978)
                     /locus_tag="AC158Q_01735"
     CDS             complement(58..978)
                     /locus_tag="AC158Q_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HIR3338519.1"
                     /translation="MPGETIAVFTKNRTNPAYEAARLGADRTAARNGARTVHFVPDRP
                     DDIGEQIALVEQAIAARPDAVVFVPVHLSALDASVSRMNAAGIPIVNYLNRLARGRFV
                     SFVGADDYRLGRDIATYLFRHLGGRGDVVMMEGVPGAVTSRERVRGFHDAARECPGIR
                     IVATRAGDYQETTARRLMAELLAELPRIDGVLSANDVMSLGILSALDSAGRSAPVIGV
                     NALPEAIHSIKAGKLLATVDFDALKISCIATEAAIRHLRGEPVPGEIILPANIVDRSN
                     YQPWDKPLEQRECPRWSDVTGNQRSVPPVS"
     gene            complement(1201..2064)
                     /locus_tag="AC158Q_01740"
     CDS             complement(1201..2064)
                     /locus_tag="AC158Q_01740"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012993.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HIR3338520.1"
                     /translation="MSDERRVPGPEVARTLRIACLQTNSGNDLTANLETLTAMAREAA
                     AGGAKFVFSGEYALMMDGSGRSMRDNAQNSSGEPALTALAGLSREIGVWHLVGSLTLM
                     SDDGRMFNRSLLISPEGVVVASYDKIHMFDATLPSGTVIRESSAYRPGERAVIAETPW
                     GKLGMTVCYDLRFPQLYRALAQRGATMLAIPASFQRETGKAHWHVLLRARAIENAAFV
                     IAPALCGDHPGKRMTYGHSLVIDPWGEVLADGGEAPGVVYAELDPAQVEKVRSMLPSL
                     THDRPFAPPTT"
     gene            complement(2078..2683)
                     /locus_tag="AC158Q_01745"
     CDS             complement(2078..2683)
                     /locus_tag="AC158Q_01745"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0042178 - xenobiotic catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HIR3338521.1"
                     /translation="MKLWFNPASPFARKVRIVAREAGVEGRIEEVGIMVSPVKPHAEL
                     ARENPLVKIPALSTELGTLYDSAVICEYLDSLHGGKAMFPKAGAERWRELRLQALGDG
                     ILEAAVLMRYEGAVRPQALQWGDWTAGQLGKVRGGLDALEQECADWGERFAIGEITAA
                     CVLGYLDFRFPDETWRKSHSALEKWYSQAARRPSMKATEPQ"
     gene            2807..3274
                     /locus_tag="AC158Q_01750"
     CDS             2807..3274
                     /locus_tag="AC158Q_01750"
                     /inference="COORDINATES: protein motif:HMM:NF015862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG/HbpS family heme-binding protein"
                     /protein_id="HIR3338522.1"
                     /translation="MSEVTLEQSGRIIDAILARGRELGCRPLSVAVTDPGAHLKAFKK
                     EDDSAMMRFEMAMGKAYAALALGRSSSLVRARNEEKPQFMSYLINASGGKIFPEGGGR
                     LIRDERGEVIGAVGVTGDIETRDEELAAHGIRAAGLKTDEDCAHLGHPVRLKN"
     gene            complement(3329..3880)
                     /locus_tag="AC158Q_01755"
     CDS             complement(3329..3880)
                     /locus_tag="AC158Q_01755"
                     /inference="COORDINATES: protein motif:HMM:NF021515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2242 domain-containing protein"
                     /protein_id="HIR3338523.1"
                     /translation="MCASTSALPVRLLCLHLLAAAVALAGCGATKEVHRKEAFEPSTP
                     FSMKIQGHSKIVCWSVKKAFLSQGYMLDRSGADSPTLTGIKDFQVDDETNVSVRLQTS
                     CADNNDGSTTVFAAASREVSEMQREKQHRSAGVGWATITVPSGSAKILRPVSRETIQD
                     ADFYQRFYSLVKRFAKEEARSAR"
     gene            complement(4017..4730)
                     /locus_tag="AC158Q_01760"
     CDS             complement(4017..4730)
                     /locus_tag="AC158Q_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338524.1"
                     /translation="MGNGEVERAMTLASRDLAAAGIDNPTPQQTEAALMGGTVTNAQG
                     QSTDMQGVLQLRSQGMGWGQIANTIGVHPSQSERGAVNSMRGRDRGQAAGAGPAGTDD
                     RFSRAQASLQSREVDRNPTVRDSARQAGSSAGGTIASARDHSRGASGAAGAQDHDSRS
                     SGALSRSEDRRNDRAPVVARNANRGGANAGAESRAEAGTFSNSGPGNSGFGLNAGASA
                     GAGADGSTGRGNGGGRGKR"
     gene            4738..5388
                     /locus_tag="AC158Q_01765"
     CDS             4738..5388
                     /locus_tag="AC158Q_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338525.1"
                     /translation="MGGVKVAVSPEPVSVTCEPLRRPSRRLVLLDPEDAEVIDPAVPV
                     AAPFVALVVVVLVLMLLLSPTILLLPVIVVPAGRKISISFTAVALALLVLVLPEVALG
                     AVVPVVAGLGAAAAGVGLLVAVGLTLVGLVVPDVVTALGAGTVVPLVAVPEPLTVAAL
                     IVVDPDAFTVPDPLLVALTVVDPDALVIALCPNAGLPRVRLSSAALTAAARVVVGS"
     gene            complement(5930..6006)
                     /locus_tag="AC158Q_01770"
     tRNA            complement(5930..6006)
                     /locus_tag="AC158Q_01770"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5970..5972),aa:Pro,seq:ggg)
     gene            complement(6082..6462)
                     /locus_tag="AC158Q_01775"
     CDS             complement(6082..6462)
                     /locus_tag="AC158Q_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014616894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR3338526.1"
                     /translation="MEASHSSRAPLPPIPAKRYFTIGEVSELCGVKPHVLRYWEQEFT
                     QLKPVKRRGNRRYYQHHEVLLIRRIRELLYDQGFTISGARNRLDELVSEPARAARQSL
                     SSRPNLATVLRKEIKSVIEVLRGQ"
     gene            complement(6446..6748)
                     /locus_tag="AC158Q_01780"
     CDS             complement(6446..6748)
                     /locus_tag="AC158Q_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HIR3338527.1"
                     /translation="MTLTKAELADLLFEKVGFNKREAKDMVESFFEEVRTALENGQGV
                     KLSGFGNFQLRDKPQRPGRNPKTGEEIPITARRVVTFHASQKLKAMVEQNYHGSKS"
     gene            complement(6760..8778)
                     /gene="pheT"
                     /locus_tag="AC158Q_01785"
     CDS             complement(6760..8778)
                     /gene="pheT"
                     /locus_tag="AC158Q_01785"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HIR3338528.1"
                     /translation="MKFSENWLRTFVNPPLATNELAQAVTMSGVEVESIGRDRDDWVL
                     TLKPTPNRGDCLGLAGIAREVAAVTGAPLSQPDIKAARATITDRLGVTLDAPQACPRY
                     CGRLVKGLDARAATPEWMARRLALSGIRPISALVDITHYVMLELGQPLHAFDAARLDG
                     GIRVRHATAGERLTLLNDMVPALTPAYLVIADESKAVALAGIMGGLDTSVSDATRDVF
                     LESAFFNPEAIAGRARLLNFSTDSSYRFERGVDFAGTLAALERATQLVIDICGGSPGP
                     VTEARATLPARDPVRLRLARVERLLGIKLDGIQVGDILRRLRFEFSAAEGEFRVTPPS
                     FRFDIAIEEDLIEELARIHGYDQVPATLPAATPTMLPVPEARRDIAALRALLVARDYQ
                     EIVTYSFVDAAWEADFAGNTAPLALANPIASQMSVMRSTLAGGLVNCLVHNISRKQVR
                     ARLFEVGRCFMTEASGAHHQPVRIGGISYGDARHEQWGLKGRPGDFYDIKGDVEALLA
                     PLAVEFRPAGHPALHPGKSAQILLGGMTAGWIGELHPRWQRKYDLPLPPVLFELDFDA
                     VSRRGLPAYKETSVFQPVRRDIAAEIDEGISYQAILEGLRREQPAIVAEIGLFDIYRG
                     TGVEKGKKSLAFRVLLQDTRKTLTDAEVESAVSQLRRVLQQQFNAKFR"
     gene            complement(8941..9930)
                     /gene="pheS"
                     /locus_tag="AC158Q_01790"
     CDS             complement(8941..9930)
                     /gene="pheS"
                     /locus_tag="AC158Q_01790"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006712692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HIR3338529.1"
                     /translation="MPQDVDAIVKEALAAFAGTDDADGLEQAKARYLGKSGALTQLLK
                     GLSRLPAAERPVFGSRVNAAKERLEAALEEGRARIQARKLEASLAEETLDVTLPGRGL
                     GVGGLHPVTRTLERIEALFRSMGFEVADGPEIETDYYNFTALNQPENHPARSMHDTFY
                     LADGKHLLRTHTSPIQIRYLEAHRPPVKIIAPGRVYRVDSDATHSPMFHQVEGLWIDE
                     HITFSDLKGVLAEFLRKFFERDDLKVRFRPSFFPFTEPSAEIDMSFGDGWLEMGGAGM
                     VHPNVLKNVNIEAEKYQGFAFGLGPDRLAMLRYGVDDLRLFFENDLRFLKQFN"
     gene            complement(9996..10355)
                     /gene="rplT"
                     /locus_tag="AC158Q_01795"
     CDS             complement(9996..10355)
                     /gene="rplT"
                     /locus_tag="AC158Q_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HIR3338530.1"
                     /translation="MPRVKRGVTARAAHKKVLQKAKGFRGRRKNVFRIANEAVMRAGQ
                     YAYRDRRDRKGEFRALWIARINAAAREHGMSYSRFMNGLKKAAVAVDRKVLADIAVLD
                     KPAFQKFVEKAKASLGA"
     gene            complement(10368..10562)
                     /gene="rpmI"
                     /locus_tag="AC158Q_01800"
     CDS             complement(10368..10562)
                     /gene="rpmI"
                     /locus_tag="AC158Q_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HIR3338531.1"
                     /translation="MPKLKTKRGAAKRFRKRAGGSIKRGKAFARHILTKKNTKRKRHL
                     RGSTGVHEADRASVGVMLPY"
     gene            complement(10636..>11285)
                     /gene="infC"
                     /locus_tag="AC158Q_01805"
     CDS             complement(10636..>11285)
                     /gene="infC"
                     /locus_tag="AC158Q_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012838978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HIR3338532.1"
                     /translation="RFFIAQEKEPRINGEFNAPEVRLIGANSEQLGVVSLAAANKLAE
                     EAELDLVEIAPTAVPPVCRIMDYGKYKYREAKKRHEAKLKQKQIIVKEIKFRPGTDEG
                     DYKIKLRNLTRFLEEGDKAKITLRYRGREMAHQELGHRLIERVRGDLEPYSVVEQYPK
                     MEGRQLIMVLAPKKTGKPAAKAPAKAGVPKKKAAVPVKAAVTEATAEAGAGIEKK"
BASE COUNT         1905 a         3682 c         3521 g         2177 t
ORIGIN      
        1 agttgggaat cctggcagcg gcgggaagcg ttctgaccgt tcacacggga aattatatta
       61 ggaaaccggc ggcacggacc gctgatttcc cgtcacgtcg ctccagcgcg ggcattctct
      121 ctgctcgaga ggcttgtccc aaggctgata attcgagcga tcgacgatgt tcgccggcag
      181 gatgatttct ccgggcaccg gctcgccccg caagtggcgg atggcggcct cggtcgcgat
      241 gcagctgatt ttcagcgcgt cgaaatccac ggtggccagc agctttccgg ccttgatcga
      301 atggattgcc tcgggcagcg cattcacgcc gatgaccggg gcgctgcggc ccgcggagtc
      361 gagcgccgag agtatgccca gcgacatgac gtcattggcc gaaagcacgc cgtcaatgcg
      421 cggcaattcc gccagcagtt ccgccatcaa ccgcctggcc gtcgtctcct gatagtctcc
      481 ggcccgggtg gcgacgatgc gtatgcccgg gcactcacgg gcggcatcgt gaaacccccg
      541 cacccgctcc cggctggtga ccgcgccggg tacgccctcc atcatcacca cgtcgcctct
      601 gccgcccagg tgccggaaca ggtaggtggc gatgtcgcgg cccaggcggt aatcgtccgc
      661 gccgacaaag ctgacgaacc ggcccctggc cagccggttg agataattca cgatggggat
      721 accggcggcg ttcatccggc tcaccgaggc gtcgagcgcg ctcaaatgca ccggcacgaa
      781 taccaccgca tcgggacgcg cggcaatcgc ctgctcgacc agcgcaattt gctccccgat
      841 gtcgtccggc cggtccggga cgaaatgaac ggtacgggct ccgttgcggg cggcggtccg
      901 gtcggcaccg aggcgcgcgg cttcgtaagc cggattggtg cggttcttgg tgaagaccgc
      961 gatcgtttcg ccgggcattg tgaaagacaa gcgatggaca ggatttacaa tgtttacagt
     1021 gataaatcga aaacccgtca acaccatcaa ttgacaggat ttacaggatt tacaagatta
     1081 agacacgcac tatcgaaaca ggactttgtt tgaatcctgt taatcttgtt aatcctgtcc
     1141 attaaggcgg ttgacgggtc tacggccttt cgatcagaaa atcctgtcta ttgccgcgct
     1201 ttaagtagtt ggcggggcga aggggcggtc gtgggtgagc gatgggagca tgctgcgcac
     1261 cttctccacc tgcgcgggat caagttcggc gtaaacgact cccggcgcct ccccgccgtc
     1321 agcgagcacc tcgccccacg ggtcgattac gagcgagtgg ccgtaggtca tgcgctttcc
     1381 gggatggtcc ccgcacaggg ctggcgcgat gacgaaggca gcgttttcga tcgcccgcgc
     1441 gcgcagcagg acgtgccagt gagccttgcc cgtttcgcgc tggaaagacg caggtatcgc
     1501 cagcatcgtc gcgccgcgct gggcgagcgc ccggtagagc tgggggaagc gcaaatcgta
     1561 acagacggtc atcccgagtt tcccccaggg cgtctcggcg atcaccgcgc gttcgcccgg
     1621 gcggtaggcg ctcgattcgc gtatcaccgt gccgctcggc agcgtggcgt cgaacatgtg
     1681 aatcttgtcg taggacgcca ctaccacgcc ttccggggaa atcagcagcg agcggttgaa
     1741 catccggccg tcgtcgctca tgagcgtgag cgatccgacc aggtgccaga ccccgatttc
     1801 gcgcgacagc ccggccagag ccgtcagggc gggctccccg gacgagttct gcgcgttatc
     1861 gcgcatgctg cgcccgcttc catccatcat cagggcatat tcccccgaaa aaacgaactt
     1921 tgcgccgcct gcagccgcct cgcgcgccat cgcggtcagc gtctcgaggt tcgcggttaa
     1981 atcgtttccg gaattggtct gcaggcaggc aatacgaagc gtgcgggcca cctcgggacc
     2041 agggacccgt cgttcatcac tcatcactca tcactcatca ctggggttca gtcgccttca
     2101 tcgacggccg ccgggccgcc tgcgaatacc atttctccag cgcggaatgg gatttgcgcc
     2161 aggtttcatc gggaaaacgg aaatccaggt agccgagcac gcaggccgcg gtaatttcgc
     2221 cgatggcgaa acgctcgccc cagtcggcgc actcctgttc cagggcgtcg aggccacctc
     2281 ggaccttgcc gagctgcccg gcggtccaat ccccccactg cagcgcctgc ggcctcaccg
     2341 cgccttcgta gcgcatgagc accgctgcct cgagaatgcc gtcaccgagc gcctgcagcc
     2401 gcaactcacg ccaccgctcc gccccggctt tcgggaacat ggctttcccg ccgtgcaggc
     2461 tgtcgagata ctcgcagatc accgccgagt catacagcgt gcccagttcc gtggacagtg
     2521 ccggtatctt gacgaggggg ttttcacgcg ccagttccgc gtgcggcttg accgggctca
     2581 ccatgatgcc cacctcctcg atgcgcccct ccacccctgc ttcccgggca acgatgcgta
     2641 ccttgcgcgc gaaaggcgat gccggattga accacaattt catacgctgc ccctcgcttt
     2701 ccgtgtcagc gcgacattct ataggaaacc tgcttgaatt ttcgcgagca cgctttacca
     2761 ttgccgttcg gccgccgcgc ggaaaccaag tcaacgggag acagtcatgt ccgaggtcac
     2821 gctggagcag tccgggcgca tcatcgacgc catccttgcc cgcgggcgcg aactcggctg
     2881 ccgccccttg agcgtcgcgg tcaccgatcc cggcgctcac ctcaaggcat tcaagaaaga
     2941 ggatgattcg gcgatgatgc ggttcgagat ggccatgggc aaggcctacg ccgcgctcgc
     3001 gctggggcgc tcctccagcc tggtgcgcgc gcgcaacgag gagaagccgc agttcatgag
     3061 ctacctcatc aatgcctccg gcggaaaaat cttcccggaa ggcggcggcc ggctgatacg
     3121 cgatgaacgc ggcgaagtca ttggcgcggt gggcgtcacc ggcgacatcg agacccggga
     3181 cgaagaattg gccgcgcacg gtattcgcgc cgcaggcctc aagaccgacg aggactgcgc
     3241 gcacctcggc cacccggtgc ggctgaagaa ctagttcagt tgcggggttt cgaataaccg
     3301 cgagcggcgc aagccgccgg gcggaacctc aacgtgcgct tcttgcttct tccttggcaa
     3361 atcgtttgac gagactgtag aagcgctggt agaagtcggc gtcctgaatc gtttcccggc
     3421 tgacgggccg gagaattttc gccgagcccg aaggcaccgt gatcgtggcc cagcctacac
     3481 cggcggagcg gtgttgcttc tcccgctgca tttcactgac ttcccggctg gcggcggcga
     3541 ataccgtggt agatccgtcg ttgttatcgg cgcacgaggt ctgcaaacgg acggagacat
     3601 tggtttcgtc gtccacctga aagtccttta tgcccgtgag ggtgggagag tcggcgcccg
     3661 accggtccag catatagccc tggctcagga acgccttctt gacgctccag cagacgatct
     3721 tgctgtggcc ctggatcttc atactgaatg gcgtgctcgg ttcgaaggcc tccttgcggt
     3781 gaacttcttt cgtcgctccg caacctgcca gcgcaacggc agccgccagg agatgcaagc
     3841 acaggagccg gacaggcaac gcggacgtcg aggcgcacat aatttctcgg tccctatgaa
     3901 agttcttttg aatgtgccag taagcgtgcc ggcgtcccgg gagagcccaa agaaaaaacc
     3961 cgccccggaa acgaagcatc acggggcggg gtccatcaga aatccatgct gcggcgtcag
     4021 cgcttaccgc ggccgccacc gttgccgcgg ccggtgctgc catccgcacc ggcaccggcg
     4081 ctcgcgccgg cgttcaagcc gaatcctgaa ttgcccggtc cgctattgct gaacgtcccg
     4141 gcctcggccc ggctctcggc gccggcattc gcaccgccgc ggttggcgtt gcgcgcgaca
     4201 accggcgcac gatcgtttct ccgatcctcg gaacggctga gcgcgccact cgaacgactg
     4261 tcatggtcct gtgcgcctgc cgcaccgctc gcgccgcggc tgtgatcgcg cgcgctggcg
     4321 atcgtcccgc ctgcggaaga cccggcctgg cgggcgctgt cgcgaacggt cgggttgcga
     4381 tcaacctcgc ggctttggag acttgcttgc gcgcggctga agcggtcatc ggtcccggct
     4441 gggcccgcgc ccgctgcctg gccacggtcg cggccgcgca tcgaattgac cgcaccccgc
     4501 tcggattggg agggatgcac gccaatcgta ttggcgatct ggccccagcc cataccctgg
     4561 ctgcgcaatt gcagcacgcc ttgcatatcg gtgctctggc cctgcgcgtt tgtcaccgtt
     4621 ccgcccatta gcgccgcttc ggtctgctgc ggcgttggat tgtcgatgcc tgcggccgcg
     4681 aggtcacggc tcgcgagcgt catcgcgcgt tcaacctcgc cgtttcccat gtgccccgtg
     4741 ggtggggtaa aggtcgcggt ttcacccgaa ccggtcagcg tgacttgcga gccgctgcgc
     4801 agaccttccc gaaggctggt gctgctcgat ccggaggatg ccgaagtgat cgacccggca
     4861 gttcccgttg ccgctccgtt cgtcgcgctg gtggtggtcg tgttggtatt gatgctgttg
     4921 ttgtcgccga cgatattgtt gttgccggtg atcgtcgtgc ccgcgggcag gaagatatca
     4981 atatcgttta ccgccgtggc gctggcgttg ctcgtgctgg tgctgcccga ggtggcgctg
     5041 ggcgccgtag ttcccgtcgt ggccggactt ggcgccgcgg cagccggggt cggactgctg
     5101 gtggcagtcg ggctcacgct ggttggactg gtcgtgccgg acgtcgtgac ggcgctcggc
     5161 gccggaacgg tcgtgccgct ggtggccgta cccgagccgc tgactgtcgc cgcgttgatc
     5221 gtggtcgacc ccgacgcatt cacggtaccc gacccgctgt tggtcgcatt gaccgtagtc
     5281 gacccggacg cgttggtaat cgcgctttgc ccaaatgcgg ggctcccgag agtgaggctc
     5341 agtagtgccg cgctcaccgc tgctgcaaga gtggtagtcg gaagttgaac cttcatcatc
     5401 aagtagtctc ctttgcgtgt catcaaataa aagggcgttc tgacggcgcg tgagttgcct
     5461 gattgggtcc gacggcgaaa caccggtgaa ccaccgggtg ccgttccgag cgacggcgct
     5521 tatggccgtc acgttcagaa ctgatacgcc cgggaccgct tgcgacaggg tcacgagctt
     5581 cagatgcagc ccgctctcgc cgttgcgagt ctcgcgtgaa ccgacccgcc ttcccacggg
     5641 agctatgcaa ttctttgcgt gccgcctgcg agtggttctg caagccgcgt acccggaaaa
     5701 ataatctcgc agaaaatcag cgactaaatt caggattgca gcgaatggcc cgtcttgctg
     5761 ttacctgacg acgacggata tcttgcacgg aaccctagga cgttgatccg taatgaacaa
     5821 aaaccgtcgc cttacggcaa caacggcaca ggctccagga agcaaccgcg gttcaggttt
     5881 tcgacttcaa aaaaatggcc tgcaagcaat cttgcaggcc atttttgtct ggtcggggcg
     5941 gtgagatttg aactcacgac caccggcacc ccatgccggt acgctaccag gctgcgctac
     6001 gccccgaggc ggcgaattat agcaaaggcc gggaaggggc ccgtcgaaaa aacgcgatga
     6061 tcaatccggc ggccgtgagc gctactggcc gcgcagcact tcgatgacgc tcttgatttc
     6121 cttgcgcagg accgtggcga ggttgggccg ggacgacagc gactgccgcg cggccctggc
     6181 gggctcggaa accagctcgt ccagccggtt gcgcgcgcca ctgatggtga acccctggtc
     6241 gtagagcaat tcccggatgc ggcggatcag cagcacttcg tggtgctggt aatagcgccg
     6301 gttgccgcgc cgcttgaccg gcttcaactg cgtgaattcc tgctcccaat agcgcagcac
     6361 gtgcggcttc acgccgcaca attcgctcac ctcaccgatt gtgaagtagc gcttcgccgg
     6421 aatcggcgga agcggagccc tggagctatg acttgcttcc atgataattc tgctccacca
     6481 tcgccttcag cttctgcgac gcgtggaacg tcacgacgcg gcgcgccgtg atcgggattt
     6541 cctcccccgt cttggggttc ctgcccgggc gttgcggctt gtctcgcagc tggaaattac
     6601 cgaagccgga aagcttcacg ccctgcccgt tctcgagcgc ggtcctgact tcctcgaaga
     6661 acgactcgac catgtccttc gcctcgcgct tgttgaaccc gaccttctcg aacaggaggt
     6721 cggcgagttc ggccttggtt aacgtcatgt tatccccttt caacggaatt tcgcgttgaa
     6781 ttgttgctgc agtacccggc gtagctgtga gacggcggat tcgacttccg catcggtcag
     6841 agtcttgcga gtatcctgta ataacaccct gaaagcaaga ctcttttttc ctttttctac
     6901 acccgttccg cggtagatgt caaacagccc gatttcggcc acgatcgcgg gctgctcccg
     6961 gcgcaggcct tccagtatcg cctgataact gatcccttcg tcgatttcgg ccgcgatgtc
     7021 gcgccgcacc ggctggaata cggatgtctc cttgtaggcc ggcagccccc gacggctgac
     7081 cgcgtcgaaa tcgagctcga acaggaccgg ggggagcggc aaatcatact tccgttgcca
     7141 gcgcggatgc aattccccga tccaacccgc tgtcataccg cccagaagga tctgcgccga
     7201 tttgcccggg tgcaaagccg gatgccctgc cgggcggaat tccacggcaa gcggcgcaag
     7261 caacgcttcg acgtcgccct tgatgtcgta gaagtcgccg ggccgcccct tcagccccca
     7321 ttgctcgtgg cgggcgtcgc cgtaggaaat tccccctatc cgcaccggct ggtggtgcgc
     7381 ccccgatgct tccgtcatga agcagcggcc gacctcgaac aatcgcgcgc ggacctgctt
     7441 gcggctgatg ttgtgaacga ggcagttgac gagtcccccg gccagcgttg aacgcatcac
     7501 gctcatctgg ctggcgatcg ggttggcgag cgccaacggg gccgtattgc cggcgaaatc
     7561 ggcctcccac gccgcgtcca cgaaactgta ggtgacgatt tcctgatagt cgcgggcgac
     7621 gagcagcgcg cgcagcgcgg caatatcccg ccgcgcctcc ggcaccggaa gcatggtcgg
     7681 tgtcgcggcc ggcaaagtgg ccggcacctg gtcatagccg tggatgcgcg ccagttcctc
     7741 gatcaggtcc tcctcgatcg cgatatcgaa acgaaagctg ggcggtgtga cccggaactc
     7801 gccttcggcc gcggaaaatt cgaagcgcag gcggcgcagg atgtcgccga cctggatgcc
     7861 gtccagcttg atgccgagca gccgttcgac gcgcgcgagc cgcaggcgca ccgggtcgcg
     7921 tgccggcaga gtcgcgcgcg cctccgtgac cgggcctggc gagccgccgc atatgtcgat
     7981 gaccagctgg gtggcgcgct cgagcgcggc cagcgtaccg gcgaaatcga cgccgcgctc
     8041 gaagcgatac gaggaatccg tggagaaatt gagcagccgc gccctgcctg cgatcgcctc
     8101 ggggttgaag aacgcgctct ccagaaatac gtcgcgcgtc gcgtcgctga ccgaggtatc
     8161 cagcccgccc atgatgccag ccagcgccac tgccttcgac tcgtcggcta tgaccaggta
     8221 cgcgggcgtg agcgccggca ccatgtcgtt cagcagcgtc agccgctccc ccgccgtcgc
     8281 gtggcgcacg cggatgccgc cgtcgagccg ggcggcatcg aacgcatgca gcggctggcc
     8341 cagttccagc atgacgtagt gggtgatatc caccagcgcg ctgatcgggc ggataccgct
     8401 caacgcgagg cgccgcgcca tccattccgg cgtcgccgcg cgggcatcga gccctttcac
     8461 cagccggccg cagtagcgcg ggcaggcctg cggggcgtcg agcgtgacgc ccaggcggtc
     8521 ggtgatcgtg gcgcgcgccg ctttgatgtc gggctgactc aagggcgcgc cggtcaccgc
     8581 cgccacttcg cgcgcgatgc cggcgagacc caggcaatcc ccgcggttgg gggtgggctt
     8641 cagcgtcagc acccagtcgt cgcgatcgcg cccgatgctt tccacctcga cgccgctcat
     8701 ggtgacggcc tgcgccagct cgttcgtcgc gagcggcgga ttgacgaagg tgcgcagcca
     8761 gttttcggaa aacttcatat caaaatagcc gccaagacgc caagacgcca ggaagatttg
     8821 ataataacga cttcaagcat cacaagatta tttgttcagg aaatcgggag agattgcgta
     8881 aaggctttct cgcactttga gcgatgatta catttcctgg cgttcttggc gtcttggcgg
     8941 ctaattaaat tgcttcagaa aacgcaggtc gttctcgaag aacagccgca aatcgtccac
     9001 gccgtagcgc agcatggcca gccggtcggg acccagcccg aaggcaaagc cctggtactt
     9061 ctcggcttcg atattcacgt tcttcagcac gttggggtga accatgcccg cgcctcccat
     9121 ctcgagccag ccatcgccga aactcatgtc gatctccgcg gagggctcgg taaacgggaa
     9181 aaacgacggt cgaaagcgca ccttgaggtc gtcgcgctcg aaaaacttcc gcaggaactc
     9241 ggcgagcacg cccttcaggt cggaaaacgt gatgtgctcg tcgatccaca ggccctcgac
     9301 ctggtgaaac atgggcgagt gcgtggcatc cgaatccacc cggtagaccc gtcccggcgc
     9361 gatgatcttc accggcgggc ggtgggcttc caggtagcgg atctggatcg gagaggtatg
     9421 cgtgcgcagc aggtgcttgc catcggcgag gtagaaggtg tcgtgcatcg agcgcgccgg
     9481 gtggttctcc ggctggttca gcgccgtgaa gttgtagtaa tcggtctcga tctcgggacc
     9541 atcggccacc tcgaacccca tcgagcgaaa cagcgcctcg attcgctcca gcgtgcgcgt
     9601 caccgggtgc agtcccccga caccgagtcc gcgtcccggc agcgtaacgt ccagcgtctc
     9661 ttcggcaaga ctcgcttcga gcttgcgcgc ctggatgcgg gcgcggccct cctcgagcgc
     9721 cgcctcgagc ctttccttgg cggcattgac gcggctgccg aacacgggac gctccgccgc
     9781 cggcagcctg gacagcccct tcagaagctg ggtcagcgcg ccggacttgc caagatagcg
     9841 cgccttggcc tgctcgagcc cgtcggcatc gtccgtgccc gcgaacgccg cgagcgcttc
     9901 cttcacgatg gcgtcaacgt cctgcggcat gggatggctg ctggaagaaa aaagggaggc
     9961 ccgtcacgcc agcctccctt cgcgcacctg cgatttcagg cgccgagact ggctttggcc
    10021 ttctcgacga acttctggaa cgccggcttg tcgagaacgg cgatatcggc cagcaccttg
    10081 cggtccaccg cgacggcggc cttcttcagc ccgttcatga agcggctgta gctcatgccg
    10141 tgctcgcggg cggcggcgtt gatgcgcgcg atccacaggg cgcgaaactc gcccttgcgg
    10201 tcgcggcggt cgcgataggc gtactgcccg gcccgcatta ccgcttcgtt ggctatgcgg
    10261 aatacgttct tgcggcggcc gcggaaaccc ttggccttct gcaggacttt cttgtgtgct
    10321 gcgcgcgcgg tgacgccgcg tttgactcgt ggcatggcgg tctcccctta gtacggcagc
    10381 atcacgccga cggacgcccg gtcggcctca tgaaccccgg tgctgccgcg caggtggcgc
    10441 ttgcgcttgg tgttcttctt ggtgaggatg tggcgcgcga acgccttgcc gcgcttgatg
    10501 ctgccgccgg cgcgcttcct gaagcgcttg gcggcgccgc gcttggtctt caacttcggc
    10561 atgtctgact cccgttcctg ttttagccta gcgccgggtg cctgccacga cggcaggact
    10621 tcgggaaccc ggcaactact tcttctcgat accggccccg gcttccgccg tggcttcggt
    10681 cactgctgcc ttcacgggga ccgccgcctt cttcttcggc acccctgctt tcgccggcgc
    10741 ctttgcggca ggcttcccgg ttttcttcgg cgccagcacc atgatcagct ggcggccttc
    10801 catcttcgga tactgctcga ctacgctgta gggctcgagg tcgcctctca cccgctcgat
    10861 caggcggtgc cccagctcct ggtgcgccat ctcgcgcccg cggtaacgaa gcgtgatctt
    10921 cgccttgtcg ccctcttcca ggaagcgcgt caggttccgt agcttgatct tgtagtcgcc
    10981 ctcgtcggtg ccgggccgga acttgatttc cttgacgatg atctgtttct gcttgagctt
    11041 cgcctcgtgc cgcttcttcg cctcacggta cttgtatttc ccgtaatcca tgatgcgaca
    11101 taccggcggc accgccgtgg gtgcgatctc gaccagatcg agctcggcct cttcggcgag
    11161 cttgttggcg gcggccaggc tcacaacgcc gagctgctcg ctgttggccc ctatcaaccg
    11221 gacttccggc gcgttgaact cgccgttgat ccgcggttcc ttttcctgtg cgatgaagaa
    11281 cctcc
//
LOCUS       DBNGSG010000072         4240 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3248550, whole genome shotgun sequence.
ACCESSION   DBNGSG010000072 DBNGSG010000000
VERSION     DBNGSG010000072.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4240)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3248550"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3645
                     /locus_tag="AC158Q_01810"
     CDS             <1..3645
                     /locus_tag="AC158Q_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019699762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HIR3338533.1"
                     /translation="YVKGGIVTWEIQQTDYPRTRWDMPNHEPRGCARGASYSWYLYSA
                     NRVKYPMVRGRLLKSWREARLSMAPVEAWASIVESDARRRDYQSVRGLGGFVRSSWDE
                     VNEIVAAANVYTIKKHGPDRVIGFSPIPAMSMVSYAAGSRYLSLIGGVCMSFYDWYCD
                     LPPSSPQVWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGAKTVAV
                     TPDYSEVAKLSDIWMHPKQGTDAAVAMAMGHVILKEFYFDKRSTYFDDYARRYTDLPM
                     LVMLKEHALPGGEKVLVPDRYVRASDFGDQLGQANNPEWKTVAFDASGKVALPNGAIG
                     FRWGPDGRADLGQWNLEAKDARHGNEVNLKLSALEGDHPGKETARVGFPYFGGIAREH
                     FTSSALKGRDGNVSGDVLVRTVPVTRVALGKDGEKREAFVATVFDLQAANYGVARGLP
                     GELAAASYDDDTPYTPAWQERITGVPREQLITVARQFAENAEKTNGKSMVIIGAAMNH
                     WYHSDMNYRGIINMLMMCGCIGKSGGGWAHYVGQEKLRPQTGWTALAFALDWIRPPRQ
                     QNSTSFFYAHTDQWRYEKLGVAEVLSPLADKKRFGGSMIDYNVRAERMGWLPSAPQLK
                     TNPLQVVRDAQAAGMDAKDYAVKALKDGTLKMSCEDPDAPQNWPRNLFVWRSNLLGSS
                     GKGHEYFLKHLLGTSHGVQGKDLGKGDARPEEVVWHDKAPEGKLDLLVTLDFRMSTTC
                     LYSDIVLPTATWYEKNDLNTSDMHPFIHPLSTAVDPAWQSRSDWDIYKGFAKKFSEVC
                     VGHLGVEKELVLTPLMHDTPAELAQPLDVKEWKKGEVDLIPGKTAPQITVVERDYPNV
                     YRRFTALGPLMGKAGNGGKGIGWNTQTEVRQLGELNGLVTGEGATHGMPKIESDIDAC
                     EVVMMLAPETNGHVAVKAWQALGKQTGREHAHLAIHREDEKIRFRDIQAQPRKIISSP
                     TWSGIESETVSYNAGYTNVHELIPWRTLTGRQQFYQDHPWMRAFGEGCVSYRPPVDLK
                     TTAGIHGVKGNGNPEILLNFITPHQKWGIHSTYTDNLMMLTLSRGGPCVWLSEDDAKR
                     AGIVDNDWIELFNLNGAIAARAVVSQRVNPGMVMMYHAQEKIVNTPGSEITGARGGIH
                     NSVTRIVLKPTHMIGGYAQLSYGFNYYGTIGTNRDEFVVVRKMVKIDWLDTPVADELI
                     RPVQAHGESV"
     gene            3642..>4240
                     /locus_tag="AC158Q_01815"
     CDS             3642..>4240
                     /locus_tag="AC158Q_01815"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HIR3338534.1"
                     /translation="MKVRAQISMVLNLDKCIGCHTCSVTCKNVWTSRPGMEYAWFNNV
                     ETKPGIGYPREWENQDKWNGGWVRRADGSIQPRQGAKWKLLMRIFANPSLPEIDDYYE
                     PFTFDYGHLQSAPEMRATPTARPRSLITGKQMDKIVWGPNWEEILGGEFSKRSQDRNF
                     DDIQKDIYGQFESTFMMYLPRLCEHCLNPSCVASCPSGS"
BASE COUNT          862 a         1352 c         1387 g          639 t
ORIGIN      
        1 tacgtcaagg gcggcattgt cacctgggaa atccagcaga ccgactaccc gcgcacgcgc
       61 tgggacatgc ccaaccacga gccgcgcggc tgcgcgcgcg gcgcgagcta cagctggtat
      121 ctctacagcg ccaaccgcgt gaagtatccg atggtgcgcg gccggctgct gaagagctgg
      181 cgcgaggcgc ggctcagcat ggcgccggtc gaggcgtggg cctccatcgt cgagtccgac
      241 gccaggcggc gcgactacca gagtgtgcgc gggctcggcg gcttcgtgcg ctcgagctgg
      301 gacgaggtca acgaaatcgt cgccgcggcc aacgtctaca ccatcaagaa gcacgggccc
      361 gaccgggtca tcggcttttc gcccattccc gccatgtcga tggtgagcta tgccgccggc
      421 agccggtatc tcagcctgat cggcggcgtg tgcatgagct tctacgactg gtactgcgac
      481 ctgccgccgt cgagccccca ggtctggggc gagcagaccg acgtgcccga atcggccgat
      541 tggtacaact ccagctacat catcgcctgg ggctccaacg tgccgcagac acgcacgccg
      601 gatgcgcact tcttcaccga ggtccgctac aagggcgcca agaccgtggc ggtcacgccg
      661 gactactccg aggtcgccaa gctctccgac atctggatgc acccgaagca gggcaccgac
      721 gccgccgtgg ccatggccat ggggcacgtg atactgaaag agttttactt cgataagcgc
      781 agcacctact tcgacgatta cgcgcgccgc tataccgatc tgccgatgct ggtgatgctg
      841 aaggaacacg cgttgccggg cggcgagaag gtcctggtgc ccgatcgtta cgtgcgcgca
      901 tccgattttg gcgaccagct cggccaggca aacaatccgg aatggaagac ggttgccttc
      961 gacgcgtccg gcaaagtggc gctgcccaac ggcgccatcg gattccgctg gggccccgac
     1021 ggccgcgccg acctgggaca gtggaacctc gaagcgaagg atgcgcggca cggcaatgaa
     1081 gtcaatctca agctttcagc gctcgagggt gatcatccgg gcaaggaaac ggccagggtc
     1141 gggttcccgt actttggcgg catcgcccgg gagcatttca cgagcagcgc gctgaagggc
     1201 cgggacggca acgtcagcgg cgacgtgctg gtgcgcacgg tgccggtgac ccgcgtcgcg
     1261 ctgggcaagg acggcgagaa gcgcgaggcg ttcgtggcca ccgtgttcga cctgcaggcg
     1321 gccaattacg gcgtggcgcg cgggctgccc ggcgagttgg ccgcggcgag ttacgacgac
     1381 gacacgccgt acacgccggc ctggcaggag cgcatcactg gcgtgccgcg cgagcagttg
     1441 atcaccgtgg cgcggcagtt cgcggagaac gccgaaaaga cgaacggcaa gtcgatggtg
     1501 atcatcggcg cggcgatgaa tcactggtat cacagcgaca tgaactaccg cggcatcatc
     1561 aacatgctga tgatgtgcgg ctgcatcggc aagagcggcg gcggctgggc gcactacgtc
     1621 ggccaggaaa agctgcggcc gcaaaccggc tggacggcgc tcgccttcgc tctcgactgg
     1681 atacgcccgc cgcgccagca gaacagcacc agcttcttct acgcccacac cgaccagtgg
     1741 cgctacgaga agctgggggt ggcggaggtg ctctccccgc ttgcggacaa gaagcggttc
     1801 ggcggcagca tgatcgacta caacgtgcgc gcagagcgca tgggctggct gccttcggca
     1861 ccgcaattga agaccaaccc gctgcaggtc gtgcgcgatg cgcaggccgc cggcatggat
     1921 gcgaaggact acgcggtcaa ggcgctgaag gacggcaccc tcaagatgag ctgcgaggat
     1981 cccgacgcgc cgcagaactg gccgcgcaac ctgttcgtgt ggcgctccaa cctgctgggc
     2041 tccagcggca aggggcacga gtacttcctc aagcatctgc tgggcaccag ccacggggtg
     2101 cagggcaagg atctcggcaa gggtgatgcc aggcccgagg aagtggtgtg gcacgacaag
     2161 gcgcccgagg gcaagctcga tctcctcgtc acgctcgact tccggatgag caccacgtgc
     2221 ctgtattccg acatcgtact gcccaccgcc acctggtacg agaagaacga cctcaacacc
     2281 agcgacatgc accccttcat ccacccgctg tccacggcgg tggacccggc gtggcagtcg
     2341 cgcagcgact gggatatcta caagggcttt gcgaagaaat tcagcgaagt ctgcgttggc
     2401 catctcggcg tggaaaagga actggtgctg acgccgctga tgcacgacac gccggcggaa
     2461 ctggcccagc ccctcgacgt gaaggaatgg aaaaagggcg aggtggatct gatcccgggc
     2521 aagacggcac cgcagatcac cgtggtcgag cgtgactacc cgaacgtcta caggcgtttc
     2581 accgcgctcg gccccctgat gggcaaagcc ggcaatggcg gcaagggcat cggctggaac
     2641 acgcagaccg aagtcaggca gctcggcgag ttgaacggcc tcgtcacggg cgaaggcgcg
     2701 acccacggca tgccgaagat cgagtccgac atcgacgcct gcgaagtggt gatgatgctg
     2761 gcgcccgaga ccaacggcca tgtcgcggtc aaggcctggc aggcgctggg caagcagacc
     2821 ggccgcgagc acgcgcacct ggcgatccat cgcgaagacg agaaaatccg cttccgtgac
     2881 atccaggcgc agccgcgcaa gatcatttcc agcccgacct ggagcggcat cgagagcgag
     2941 acggtttcgt acaacgccgg ctacaccaac gtgcacgagc tgattccctg gcgcacgctg
     3001 accggccgcc agcagttcta ccaggatcac ccctggatgc gcgcgttcgg cgagggctgc
     3061 gtcagctacc gcccgccggt ggacctgaag accacggccg gcatccacgg agtgaagggt
     3121 aacggcaatc ccgagatcct gctgaacttc atcacgccgc accagaagtg gggcatccac
     3181 tcgacctata ccgacaacct gatgatgctc acgctctccc gcggcgggcc gtgcgtctgg
     3241 ctctccgaag acgacgcgaa acgcgcgggc atcgtggaca acgactggat cgagctgttc
     3301 aacttgaacg gcgcgatcgc ggcgcgcgcg gtggtcagtc agcgcgtcaa ccccggcatg
     3361 gtgatgatgt atcacgcgca ggagaagatc gtgaatacgc cgggttccga gatcacgggc
     3421 gcgcgcggcg gcatccacaa ctcggtaacg cgcatcgtgc tgaaacccac gcacatgatc
     3481 ggcggctacg cgcaattgag ctacggcttc aactactacg gcacgatcgg caccaaccgc
     3541 gacgagttcg tcgtggtgcg caagatggtg aagatcgact ggctggacac gccggtcgcc
     3601 gatgaactga tccggcccgt ccaggcccat ggagaatcgg tatgaaagtc cgcgcccaga
     3661 tcagcatggt gctgaacctg gacaagtgca ttggatgtca cacttgttca gtaacctgca
     3721 agaacgtgtg gaccagccgg cccggcatgg aatacgcctg gttcaacaac gtcgagacca
     3781 agcccggcat cggctatccc agggaatggg aaaaccagga caagtggaac ggcggctggg
     3841 tccgcagggc cgacggctcc atccagccgc ggcagggggc caagtggaag ctgctgatgc
     3901 gcatcttcgc caaccccagc ctgccggaaa tcgatgacta ctacgagccg ttcacgttcg
     3961 actatggcca cctgcaaagc gcaccggaga tgcgcgccac gccgaccgcg cggccgcgca
     4021 gcctgattac cggcaagcag atggacaaga tcgtgtgggg cccgaactgg gaggaaatcc
     4081 tcggcggcga gttcagcaag cgctcccagg acaggaactt cgacgacatc cagaaggata
     4141 tctacggcca gttcgagagt accttcatga tgtacctgcc gcgcctgtgc gagcactgcc
     4201 tgaatccctc gtgcgtcgcg agctgtccct cgggctcgat
//
LOCUS       DBNGSG010000073         7759 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1382133, whole genome shotgun sequence.
ACCESSION   DBNGSG010000073 DBNGSG010000000
VERSION     DBNGSG010000073.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7759)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7759)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7759
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1382133"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(578..889)
                     /locus_tag="AC158Q_01820"
     CDS             complement(578..889)
                     /locus_tag="AC158Q_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338535.1"
                     /translation="MSTFATAGWIVMAVLWLLVAIYVFRLIRQNNSERLVRCPETGAI
                     TLVSISSAAWRDGNGSAPEVRHCALWQDGQDCVQGCLTRQAEMSPGYRVNLQALRPFT
                     K"
     gene            1081..2409
                     /locus_tag="AC158Q_01825"
     CDS             1081..2409
                     /locus_tag="AC158Q_01825"
                     /inference="COORDINATES: protein motif:HMM:NF014774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HIR3338536.1"
                     /translation="MSETFAEALEARTQEVLDRCTRCGRCFEVCPMTAPAGLAGEKPA
                     DVVGGVLDVLRGGEGAPESVRWAQVCSGSGSCIPACPEGVNPRFMLALTRVAVQRRAT
                     AEEQHKKGSAAFANMGRGVRVLSRMQLPAEVLKRFRGESPAEAAPEVVFYTGCNVLKT
                     PHIVLLALDILDALGVTYRVMGGPGDCCGVLQFRAGDLGASGKIAYGTTDRFAATGAG
                     KVLAWCPTCAIQVGEYVLPGRSDKPAYELEAFAVYLAGRLERLRPLMRHRVEKRVGLH
                     EHPGLAGVCESVVAILKAIPGLEYVELEQPQVGYMCNTLQPLPAFKREVHQGILEAAA
                     AAKVDALAGVYHACHRELCSHERDWPFEVVNFLELIGEALGIRREDSFKRLKKMQDAD
                     AILADVMDLVERHGLALEEVRAVIEKDLLGEQPLPLRGAGVERDTASAPG"
     gene            complement(2546..3016)
                     /locus_tag="AC158Q_01830"
     CDS             complement(2546..3016)
                     /locus_tag="AC158Q_01830"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3338537.1"
                     /translation="MEHVETSINVRCPLRAVYDQWTRFEEFPEFIPGVLEVHQIDDTH
                     VHCHAEILGEETELDAEIIDQVPNDHITWKSSSGVPSVGIVRFEPLGPALTRVQLVIA
                     YEPGAATDDVRDALQALSVRIQETVEDFKWFIEDRDRESRGRPRTNIGTGDSDT"
     gene            3426..3803
                     /locus_tag="AC158Q_01835"
     CDS             3426..3803
                     /locus_tag="AC158Q_01835"
                     /inference="COORDINATES: protein motif:HMM:NF033674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NirD/YgiW/YdeI family stress tolerance protein"
                     /protein_id="HIR3338538.1"
                     /translation="MRKLMYGVPIVLLFGVTPAAAQKPVAPITSAQSVAAAKDAQIVK
                     LRGEIVSKQSGSEYVFSDGTGSVVVAIGEKVLKGKALPSGTPVEIVGQVDKGILRQSK
                     VEVQSVTVLAASGPPGEPADSVM"
     gene            complement(3922..4458)
                     /locus_tag="AC158Q_01840"
     CDS             complement(3922..4458)
                     /locus_tag="AC158Q_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3338539.1"
                     /translation="MERIEKIIDVNCPVRMVYDQWTQFEEFPQFMADAYEVRQIDDTH
                     VHWHARIRGKDKEWDAEITEQVPEDRISWKSPSGAPRAGTVRFEPLGSGATRVRLVMA
                     YEPEGPLESIGDAIGVIDSRVQHTVEKFKLYIESRARDTGPWRGEFSGSLRKMRANVI
                     RGGPLGGVGDVWPHCGDA"
     gene            4827..5108
                     /locus_tag="AC158Q_01845"
     CDS             4827..5108
                     /locus_tag="AC158Q_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338540.1"
                     /translation="MELALLAIAALLLGVLAVYAQLGIPRHAASRGASLLTRLALVVT
                     GTALGAVSSTLYPADPVRAVLFFLIGFGVVHFPAACIVFVKHLQRTGGL"
     gene            5216..5992
                     /locus_tag="AC158Q_01850"
     CDS             5216..5992
                     /locus_tag="AC158Q_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338541.1"
                     /translation="MRAMAFLVLAGWLIDAAAANNYAEMRSAAVKRCEAIDPSEYQSG
                     LAFNPEGYRSLYVRSACFQRAAVMFRDESLCAQVRERWSLLWSSWGYSGKRCRALVTE
                     GAAADRKSLEEMKSRYRKAAVTLRDFRIEMNGNGRDFDIIPSFDPGYAHGYTLRLEIV
                     DAGAAGESVPVATSGFHLGGNENIRLFVTQADMRRRFPRFELARPYRVRGTLILEVGN
                     GGQGGMWSDAFIERVFPVSARSKSLVKEIAFGVAGAAPSR"
     gene            complement(5989..6162)
                     /locus_tag="AC158Q_01855"
     CDS             complement(5989..6162)
                     /locus_tag="AC158Q_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338542.1"
                     /translation="MPKDPPKIKRCPECRRLVEAETMRPLSRTRFGGGVRFACPDCFK
                     RVMAMRKAVAGRR"
     gene            6472..6780
                     /locus_tag="AC158Q_01860"
     CDS             6472..6780
                     /locus_tag="AC158Q_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338543.1"
                     /translation="MAADKPKTEDAPEIDLDEVRKLVEALERDLEKVQGGTRDVQVLR
                     DEVATLRNVLDSPVRRHHWVRDGLHGIRDGFENVVDSAIAEGLRAGRYVAEIGRILGL
                     "
     gene            complement(7028..7201)
                     /locus_tag="AC158Q_01865"
     CDS             complement(7028..7201)
                     /locus_tag="AC158Q_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338544.1"
                     /translation="MTKDPSRIKRCPECHRLVEAATMRPLSHTRLGGRVRVACADCFR
                     RVMDLRKAVAARL"
     gene            complement(7333..7689)
                     /locus_tag="AC158Q_01870"
     CDS             complement(7333..7689)
                     /locus_tag="AC158Q_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338545.1"
                     /translation="MSPDLYAKVTGGIWGFILGAGIAMIIGFQVAGWSTSTASQKMSD
                     QAVLASYASICVAQFMSAPNQKAKAREFQGTESYLRSDYIEKGGWDRMPGQEKAAWGV
                     SSACVAAIEAQRTPGA"
BASE COUNT         1372 a         2431 c         2590 g         1366 t
ORIGIN      
        1 caacaggatc aacgagacag cgatgcggac catggcctcc ctcccaggta tcggcagtgc
       61 gggaaagtat ggaccggcag aattaaaggc acaaggcgca agggataagg ggaaggagtg
      121 tcgaggaagg agtgtcgagg gaggaaacga gggacgagga ttgtgtgtcg agtgaacaaa
      181 acgctacaga gctctacccc tcgtcacccg acactcgaca cctgatacac gagcaacgag
      241 caacgagaaa cgagtcactt ctttctgcga aggcaaaagg ctaaaggcaa gaggcaagag
      301 ggaaaaggga aggtgcccca atgttgaaaa acgccgtcac tcccgcgaag gcgggagtcc
      361 agggaatcca cctgtatgcg gaacaacgag taacgggatt cccgcctccg cgggactgac
      421 gttatcgttc tgtgcgacac gtttttcaac gccctcctaa cccccgtgac aagtggcgag
      481 ggtgaggttc cttgaaaggc gttcctacgt gtcctttgtg tcctccgtgt cgaggctttt
      541 ctgtagcttc gccgctccgc cgcccgatgc tttgcagcta cttggtgaag ggtcgcagcg
      601 cttgcaggtt cacccggtag ccgggggaca tttccgcctg gcgcgtgagg cagccctgga
      661 cgcagtcctg accgtcctgc cagagcgcgc aatgccggac ttccggcgcg ctcccgttgc
      721 cgtcacgcca cgcggcggac gaaatgctga ccagcgtgat cgccccggtc tcggggcacc
      781 tcaccaggcg ctcgctattg ttctgccgga tgagcctgaa gacataaatg gcgaccagca
      841 gccagagcac tgccatcacg atccatcctg ccgtggcaaa ggttgacata aatcctctcc
      901 ttttcgtgcc gataccgctt catgatgatc tcgccgggcg tccccggtat tgatctggcg
      961 caaaccctgg gcgaccggac gctcgaaggc gcgttccagc ccttcggtcg ggtaacgcgc
     1021 tgatctgcct cgatcttttt tctggctgtg cggctgccgc aggcggtaaa cttggaaaac
     1081 atgagcgaaa catttgccga agcgctggaa gcccgcacgc aggaggtgct ggaccgttgc
     1141 acgcgctgcg ggcgctgctt cgaggtgtgc ccgatgaccg cgcccgccgg tctcgcgggc
     1201 gagaagccgg ccgacgtggt cggcggggtg ctggacgttc tgcgcggcgg cgaaggcgcg
     1261 ccggagtcgg tgcgctgggc gcaggtgtgc tcgggcagcg gcagctgcat ccccgcgtgc
     1321 ccggagggcg tgaacccgcg gttcatgctc gccttgacgc gcgtcgccgt gcagcggcgc
     1381 gcgactgcgg aagagcagca caagaagggc agcgccgcgt ttgccaacat gggccgcggc
     1441 gtgcgcgtgc tctcgcgcat gcagctcccg gccgaggtgt tgaagcgctt tcgcggcgag
     1501 tccccggcgg aagccgcgcc ggaagtggtg ttctacaccg gctgcaacgt gctgaagacg
     1561 ccgcacatcg tgctgctcgc gctcgacatc ctcgacgcgc tcggcgtgac ctaccgtgtg
     1621 atgggcgggc cgggcgactg ctgcggcgtg ctgcagtttc gcgccggcga cctcggcgcc
     1681 tcgggaaaga tcgcctacgg caccaccgac cgcttcgccg ccaccggcgc gggcaaggtg
     1741 ctcgcgtggt gcccgacgtg cgcgatccag gtcggggaat acgtgttgcc cgggcgcagc
     1801 gataaacccg cctacgagct cgaggccttc gccgtgtacc tcgcgggccg gctcgagcgg
     1861 ctgaggccgc tcatgcgcca ccgcgtcgag aagcgcgtcg ggctgcacga gcacccgggt
     1921 ctcgcgggcg tgtgcgagtc cgtggtcgcg atattgaagg cgatcccggg gctcgagtac
     1981 gtcgagctcg agcagccgca ggtgggctac atgtgcaaca cgctgcagcc gctgcctgcc
     2041 ttcaagcgcg aagtgcacca gggaatactc gaagccgcgg cggcggcgaa ggtggacgcg
     2101 ctcgccggcg tctaccacgc ctgtcaccgc gagctgtgca gccacgagcg ggactggccg
     2161 ttcgaggtgg tgaacttcct cgaactcatc ggcgaggccc tcggcatccg gcgcgaggac
     2221 agcttcaagc gcctgaagaa gatgcaggac gccgacgcga ttctcgcgga cgtgatggat
     2281 ctcgtggagc ggcacgggct cgcgctcgag gaagtgcgcg cggtgatcga gaaggacctc
     2341 ctcggcgagc agccgctgcc gctgcgcggc gccggcgtgg agcgcgacac cgcctccgcg
     2401 ccgggctgac cgcctgaagt cgaaagtcga aagtcgaaag tcgaaagtcg aaagtcgaaa
     2461 gtcgaaaatc gaatgtcgaa agtcggaagg cggaaccgtc accccgtcat ttcgtcatcg
     2521 catcggcgcg ccgatggcgc ggtcctcacg tgtcgctgtc gccggtgccg atgttcgtgc
     2581 gcggccgccc gcgggactcc cggtcgcggt cctcgatgaa ccacttgaag tcctcgacgg
     2641 tttcctggat gcggacgctc agcgcctgaa gggcgtcgcg gacgtcgtcg gtcgcagccc
     2701 cgggctcgta ggcaatcacc agctgcaccc gcgtgagcgc cgggcccagc ggctcgaagc
     2761 gcacgatacc gacgctggga acgcccgacg agcttttcca cgtgatgtgg tcgttgggca
     2821 cctggtcgat gatttccgcg tccagttcgg tttcttcgcc caggatctcg gcatgacaat
     2881 gcacgtgggt gtcgtcgatc tggtgcactt ccagcacgcc cgggatgaac tccggaaatt
     2941 cctcgaaccg tgtccactgg tcgtagacgg cgcgcagcgg gcagcggacg ttgatgctcg
     3001 tttccacatg ttccataggg actcctttca attgatcgtc gtcaggcacc gtgtcatcgc
     3061 ttcggccgtg gcggcgcgct cgatgaatgg cggtttcgat ttctgatcgc gctccccgca
     3121 agcatcggcc gtgccagacc gcttgcgcgc cgaaccgttt taccgtcacg gggcacattc
     3181 gatgtatgac gatgaaaact tcataagttc ccggggagga acagaaagcg gaattcccga
     3241 agacagcgcg actcgcgaat caacctcggc gaatccggtc tcccatcacc ggctgccgcg
     3301 ctcatcattt gcgcccccgt gacggctgcg atcgccaacg ttctgatttc cgcgcggaat
     3361 catttgcggg aactgtattt tcgccgcgcg ttctacccgg atatcacgtg aggaaggaga
     3421 aggacatgcg aaagctgatg tacggcgttc cgatagtgtt gctgtttggc gtcaccccgg
     3481 ccgcggcgca gaaacccgtc gcgccgatta ccagcgcgca aagcgtggcg gccgccaagg
     3541 acgctcaaat cgtgaagctc cgcggcgaga tcgtgagcaa gcagagcggc agcgagtacg
     3601 tattctccga cggcacgggc agcgtggtcg tggcgatcgg cgaaaaagtg ctgaagggca
     3661 aagcgcttcc ctccggcacg ccggtcgaga tcgtcggcca ggtcgacaag ggcatcctga
     3721 ggcagtcgaa ggtggaggtg cagtccgtga cggtgctcgc cgcgagcggc ccgccgggag
     3781 agcccgcgga ttcagtgatg tagtgccggg tggtcggggc gccggccact gcgcttgctt
     3841 cagcggctga tcgctgcgcg accggttccc ggacggcgcg gggaagccgg tcgggcatgg
     3901 ccggcggcgc cggtgcttct cctacgcgtc gccgcagtgg ggccagacgt cgcccacgcc
     3961 gccgaggggg ccgccgcgga tcacgtttgc ccgcattttc cgcaggctgc cgctgaattc
     4021 cccgcgccat ggcccggtgt cccgcgcgcg gctctcgatg tacaacttga atttctcgac
     4081 ggtatgctgc acgcgcgagt cgattacccc gatcgcatcg ccgatgctct cgagcgggcc
     4141 ctcgggctcg taggccatta ccagccgcac gcgggtggcc ccggagccga gcggctcgaa
     4201 gcgcacggtg ccggcgcggg gcgcgccgga cgggctcttc cacgaaatgc ggtcctcggg
     4261 cacctgctcc gtgatctcgg catcccactc cttgtccttg ccccggatcc gggcatgcca
     4321 gtgcacgtgg gtgtcgtcga tctgccgcac ctcgtacgcg tccgccatga actgcgggaa
     4381 ttcctcgaat tgggtccact ggtcgtagac catgcgcacc gggcagttga cgtcgatgat
     4441 tttctcgatc cgttccatga tggctcctcc aagtcgctat cgccggcgtg aacacggccc
     4501 tggcgcgcgg ggcgccacgg ccaatgcgct tcttattgcg gcaacagggt tatcccttca
     4561 ctgaactgca cacgaagttt ttgattgaag caaccaatat gcccgcgcgc ggcgccgggc
     4621 agagtccttg atttcgcgcc aggttatttt ttgacgaagc cgcgcagtgt tgccgccggg
     4681 cgacacgccg ccgcggcatc gggcctgttt tgctcacgcg aaatgaaatt ttctcacccg
     4741 ggctatgcac cgcaagcccg caagaccggc ctggacggga tgaccgggcg cctgaacgga
     4801 cggcggcggg gccctgtcgt accggcatgg agcttgccct gctggcgata gcggcattgc
     4861 tgctgggggt gctggcggtc tacgcgcagc tcgggatccc gcgccatgcg gcgagccgcg
     4921 gcgcgtccct gctcacccgc ctggccctgg tggtgacggg gactgcgctc ggagcggttt
     4981 cgtccacgct ttatcccgct gaccccgtgc gcgccgtgct gttcttcctg atcgggttcg
     5041 gcgtagtcca tttcccggcg gcgtgcatcg tgttcgtcaa gcacctgcag cgcacgggcg
     5101 ggttgtaagg ccgcgcccgg agggggactc ccgggaggaa acagaaaccg cgcgcccatt
     5161 ccccgtcatg gcgtcgccgc ctcgccacgc cgctcggtat gatattcgta tgctcatgcg
     5221 cgcgatggca ttcctggtcc tggccggatg gctgatcgat gcggcggcgg ccaacaacta
     5281 tgccgagatg cggtcggccg cggtcaagcg ctgcgaagcg atcgacccgt cggagtatca
     5341 gagcggcctt gctttcaatc ccgaggggta ccgttcgctg tacgtccgct cggcgtgctt
     5401 ccagcgcgcc gcggtgatgt ttcgcgatga atccctgtgc gcgcaggtca gggagcgctg
     5461 gtcgctcttg tggtcgagct ggggctattc cggcaagcgc tgccgggcgc tcgtgaccga
     5521 aggcgcggcg gcggaccgca aatccctgga agagatgaaa agccggtacc ggaaagccgc
     5581 cgtgaccctg cgcgacttcc gcatcgagat gaacggcaac gggcgcgact tcgacatcat
     5641 tccatcgttc gatcccggct acgcccacgg gtacacgctg cgcctcgaaa tcgtggacgc
     5701 gggggcggca ggagagagcg tgccggtcgc cacgtccggc tttcacctgg gcgggaacga
     5761 gaatatccgc ctgttcgtca cccaggcgga tatgcgcagg cgcttccccc gattcgagct
     5821 tgcgcgcccg taccgcgtcc ggggcacgct gatcctggaa gtcggaaacg gcgggcaagg
     5881 cggaatgtgg agcgacgctt ttatcgagcg cgtcttcccg gtctccgcca ggtcgaaatc
     5941 cctggtgaag gaaattgcgt tcggtgtggc cggcgcggcc ccttcccgtt agcggcgccc
     6001 ggccacggcc ttgcgcatgg ccatgacgcg cttgaagcaa tcggggcagg cgaagcgcac
     6061 gccgccgccg aaccgcgtgc gcgacagggg gcgcatggtc tcagcctcca ccagccgccg
     6121 gcattccggg caccgcttga tcttcggggg gtctttgggc actcgcacgt ttagtaaagg
     6181 aaggcaggca gggctgccaa gcgttcaggg tagcagaacg ggggggcgga agtcgaaggg
     6241 cgggaggatg agggagggag tgtcgaggaa tgagtgtcgg gtgaaaaaac gtactggtgg
     6301 cttttttccc tcgactctcg accctcgacc ctcgaccttc gacactcgtc acccgacacc
     6361 cgtcacccga cacccgattc acgagaaacg agtcacgccg ttcaaaagcg tgccgtttcc
     6421 cagggggcgg gactggtgac agaatagggc caaccccgag gagagccgac agtggcagcg
     6481 gacaagccga agacggaaga cgcacccgag atcgacctgg atgaagtgag aaagctggtg
     6541 gaagccctgg agcgcgacct cgagaaggtg cagggcggga cgcgagatgt ccaggtcctg
     6601 cgggacgagg tggcgacgct caggaacgtg ctcgactccc cggtgcgcag gcaccactgg
     6661 gtgcgcgacg gcctgcacgg gatccgggac ggcttcgaga atgtcgtgga ctcggcgatc
     6721 gccgagggct tgcgcgcggg ccgctacgtc gccgaaatcg ggcggatact gggactctaa
     6781 cttggaaggc aaaaggcaaa aggcaaaagg caaagtgccc tgaatcccgt gaaacgggaa
     6841 acgtgaatcg gaaaacgtag aatcgatcgg gccgttacgt tttacggttc acgattcacg
     6901 aatcacgaga aacgagcaac gcctgtcctg agcatagtcg aagggagaaa cgagccactt
     6961 ctttcggcga agacagaagg ctaacccctg agttgcgcca ggagccgccc gtcaccgggt
     7021 tgctgcgtca gaggcgcgcg gcgacggcct tgcgcaggtc catgacgcgc ctgaagcaat
     7081 cggcgcaggc aacgcggaca cggccgccga gccgcgtgtg cgacaagggt cgcatggtcg
     7141 cagcctccac caggcggtgg cattcggggc accgtttgat ccgggagggg tctttggtca
     7201 ttcgcgcctt ggtccgatcc ggaccgaacg tcagttacaa ggctcaaggt acaaggttca
     7261 agttacaagg ttcaaggtgc tttgcttgta ccttgttccg tgtaccttga tccttgtcat
     7321 tggcatcctt cgtcacgcgc cgggcgtgcg ctgcgcttca atggccgcga cacaggcact
     7381 ggatacgccc caggcggcct tttcctgccc cggcatccgg tcccagccgc ctttttcgat
     7441 gtagtcggag cgcaggtagc tttccgtgcc ctgaaattcc ctggccttgg ccttttggtt
     7501 cggggcactc atgaactggg cgacgcagat cgacgcgtaa cttgccagca ccgcctggtc
     7561 gctcatcttc tgggacgcgg ttgaagtcga ccaaccggcc acctggaagc cgatgatcat
     7621 cgcgatgccc gctccgagaa taaaccccca aattccaccc gttaccttcg cgtacagatc
     7681 tggcgacatg gtcgtacctc ttcaaaaagt ggtgaagtcc gcaggctccc gcgtagtccg
     7741 acgcggaaac cgggaccgg
//
LOCUS       DBNGSG010000074         4415 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2401222, whole genome shotgun sequence.
ACCESSION   DBNGSG010000074 DBNGSG010000000
VERSION     DBNGSG010000074.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4415)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4415
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2401222"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1073
                     /locus_tag="AC158Q_01875"
     CDS             <1..1073
                     /locus_tag="AC158Q_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012897.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HIR3338546.1"
                     /translation="TIERDGQMRSLADIGAAVLANACGPCIGQWRRAKEASAAPNTIV
                     TSYNRNFPARNDGQPTTMNFIASPEIVTAFAVAGRLSFNPLADTLTDAGGKPFKLAPP
                     QPAPEVPVKNFDRGQSAYVAPPADGSRVELKVKPDSGRIQLMQPWPAWDGTDFTDMPV
                     LIKTKGKTTTDHISPAGPWLRYRGHLDKFSDNLLEGGTNAWSGERGKSRNALTGKTGE
                     PVAANARHYQSQKVRWLIVGDANYGEGSSREHAALSPRLLGGVAVIARSFARIHESNL
                     KKQGLLALTFPDPVDYDRIREDDRVSLVGLKDMAPGKPVECRVRHADGKIETLMLNHT
                     FSAPQIEWFRAGSALNLVHKDG"
     gene            complement(1200..1994)
                     /locus_tag="AC158Q_01880"
     CDS             complement(1200..1994)
                     /locus_tag="AC158Q_01880"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3338547.1"
                     /translation="MDLGIKGETALVVGASEGIGYESARGLIEEGARVLICSRSADKL
                     KSAAARLKQATGFDAPWFAADVTRAQDVGKLGAWLGRQAAALDILVSAVGGSRRALFG
                     ELDDAAWIASYEFNVLGTVRVIRAALERLKQAKSGGRIVILGAAGARMPYPHQVVSNV
                     HKAGLIALVKTLGAEFQPFNIRVNSVSPGRTLTSLWTTRAEQLAKERGVATEEIIREF
                     SREIPQQRFGRPEEIAAMVVFLASHKASYVNCQSVLVDGGIARGLV"
     gene            complement(2120..3088)
                     /locus_tag="AC158Q_01885"
     CDS             complement(2120..3088)
                     /locus_tag="AC158Q_01885"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3338548.1"
                     /translation="MKGLSRLATLALVAAVSAPFAALAQNYPAKPVRVIIVFPPGGSN
                     DVTGRIVFQKVTENVGQQFVIENRGGAAGTIGADVVAKSPPDGYTIMVQSATHIANAH
                     LYKKLPYDTLGDFIGITAMARQVGILVVHPSLPVKNTKEFIALARARPGEVVYGSSGN
                     GSFVHLTMALLNTMTNTKMVHVPYKGGGPVNIAIASGEVQAIIATIASVVPNLNSKRV
                     RAIAVTSDKRTTQYPNVPALAETIPGYEFTAWVGAFAPKGTPKPVIDKLNAEIRKALA
                     DPGVSSKLSAQTLDPMPMTPEQFAARLKSDYDKYEKVVKLSGATLD"
     gene            complement(3252..4223)
                     /locus_tag="AC158Q_01890"
     CDS             complement(3252..4223)
                     /locus_tag="AC158Q_01890"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3338549.1"
                     /translation="MKCFLSRVLPCVLAACFFPLTAFAQAYPAKPVRVIVVFPPGGSN
                     DVVARIVFQKMTETMGQQFLIDNRGGGAGSLGATVVAQSPADGYTLMVHSTTHIANAH
                     VYGSKLQYDTLRDFIGITPLARQVAMAVVHPSLPVNSIKELIVLAKKRPGEIVFGSAG
                     HGSYIQLTVELLMSMTGTKMLHVPFKGGGPAGTALVSGETQLQIATIASVFTHVKAGR
                     VRAIGVTSEKRSPQFPDVPAIAETVPGYEFTAWVGAFAPAKTPRPIVDRLNAEFKKVV
                     SDPDVASKLTAMTLDPMHMTVPQFAERLQSDHERYGKMLRALGTKVN"
BASE COUNT          773 a         1499 c         1385 g          758 t
ORIGIN      
        1 cgacgatcga gcgcgacggc cagatgcgct cgctcgccga catcggcgcg gcggtgctgg
       61 cgaacgcctg cggcccctgt atcggtcagt ggcgcagggc gaaggaagcg tccgccgcgc
      121 ccaacaccat cgtcacctcg tacaaccgca atttcccggc acgcaacgac ggccagccga
      181 ccacgatgaa cttcatcgcg agccccgaga tcgtcaccgc gtttgccgtt gccggccgcc
      241 tgtcgttcaa cccgctggcg gacacgctga ccgatgccgg cggcaagccg ttcaagctcg
      301 cgccgccgca gcccgccccc gaggtgccgg tgaagaattt cgaccgcggc cagtctgcct
      361 acgtcgcgcc gccggcggac ggcagccggg tcgagctgaa ggtgaaaccg gacagcgggc
      421 gcatccagtt gatgcagccg tggccggcat gggacggtac cgacttcacc gacatgccgg
      481 tgctgatcaa gaccaaaggc aagaccacga ccgaccacat ctcgccggcc ggaccgtggc
      541 tgcgctaccg cgggcatctc gacaagttca gcgacaacct gctggagggc ggcaccaacg
      601 cctggagcgg cgagcgcggc aagagccgga acgcgctgac cggaaaaacc ggcgaaccgg
      661 tcgccgccaa cgcccgccat taccagtcgc agaaggtgcg ctggctgatc gtcggcgacg
      721 ccaactacgg cgaaggcagc agccgcgaac acgcggcact gtcgccgcgg ctgctgggcg
      781 gcgttgcggt gatcgcccgc agcttcgcgc gcatccacga gtcgaacctg aagaagcagg
      841 gcctgctcgc ccttaccttc ccggacccgg tggactacga ccgcatccgc gaggacgatc
      901 gcgtgagcct cgtgggattg aaggacatgg cgcccggcaa gccggtcgaa tgccgcgtgc
      961 ggcacgccga cggcaaaatc gagacgctga tgctgaacca caccttcagc gcgccgcaga
     1021 tcgaatggtt ccgcgccggt tcggcgctca acctcgtgca caaggacggc tagacgagtc
     1081 ctcgcgcaat gccacccatc cgatccctat cgccggtcgg cacgccggcg atcaggaatc
     1141 tcccctctcc gctcgggaga ggggcggggg tgagggattg ctgaagattg atttcgccat
     1201 caaaccaacc ctctcgcgat acccccatca accagaacgg actggcagtt gacgtagctc
     1261 gccttgtgcg aggcgaggaa caccaccatc gcggcgatct cctcgggccg gccgaagcgc
     1321 tgctgcggta tttcacggga gaactcccga atgatttctt ccgtcgcgac gccgcgctcc
     1381 ttcgccagtt gctccgcgcg cgtcgtccac agggaagtca gcgtgcggcc gggcgagacg
     1441 gaattgacgc ggatattgaa cggctggaac tcggcgccca gcgtcttcac cagcgcgatg
     1501 aggcccgcct tgtgcacgtt cgataccacc tggtgggggt acggcatgcg cgcgccggcg
     1561 gccccgagaa tgacgatgcg cccgccggat ttggcctgct tgagacgttc caaagcggcg
     1621 cgtatcaccc gcaccgttcc gagcacgttg aactcgtagc tcgcgatcca cgcggcatcg
     1681 tcgagctccc cgaacagcgc gcgccggctg ccgcccaccg cgctcaccag gatgtcgagc
     1741 gcggccgcct gccgacccag ccaggccccg agcttcccga catcctgcgc ccgggtgacg
     1801 tccgccgcga accagggcgc gtcaaaaccg gtcgcctgct tcaggcgcgc cgcggcagac
     1861 ttgagcttat ccgcgctgcg cgagcagatg aggacacgcg ccccctcctc gatcagcccc
     1921 ctggcgcttt catagccgat accctcgctc gccccgacga caagcgcggt ctcacccttg
     1981 atgccgagat ccatgcacag agtctatctt gttgacaggc gcgtgatgcg ccgtgaccag
     2041 gacggagcgg aatgcacaaa aagaaaccgg cagtcctgaa actgccggtt tccctgcatg
     2101 ccgcaacccg cggacgccgc tagtccaatg tcgcgcccga aagcttgacc accttctcgt
     2161 acttgtcgta gtccgacttc agtcgcgcgg cgaactgctc cggcgtcatc ggcatcgggt
     2221 ccagcgtctg cgccgagagc ttcgacgaaa cgccgggatc ggccagcgcc ttccggattt
     2281 cagcgttgag cttgtctatg accggcttgg gcgtcccctt cggtgcgaac gcgcccaccc
     2341 aggcggtgaa ctcgtagccg ggaatggtct cggcaagcgc gggcacgttc ggatactgtg
     2401 ttgtccgctt gtccgacgtc acggctatcg cgcgaacccg cttgctgttg aggttgggaa
     2461 ccacggacgc gatcgtggcg atgatcgcct ggacttcgcc ggatgcgatg gcgatattga
     2521 cggggccgcc gcccttgtac ggcacgtgca ccatcttggt gttggtcatc gtgttgagca
     2581 gcgccatcgt caggtgcacg aagctgccat tgccggagga gccgtatacg acctcgcccg
     2641 gccgcgccct ggcgagcgcg atgaactcct tggtgttttt caccggcagc gagggatgca
     2701 cgaccagtat gcccacctgc cgcgccatgg cggtaatgcc gatgaagtcg cccagcgtgt
     2761 catagggcag cttcttgtac aagtgcgcgt tggcgatgtg ggtcgccgac tgcaccatga
     2821 tcgtgtagcc gtccggcggg ctcttggcaa cgacgtccgc gccgatggtg cccgccgcgc
     2881 cgccccggtt ctcgatcacg aattgctgcc ccacgttctc cgtcaccttc tggaacacga
     2941 tacggccggt aacgtcgttg gagccgccgg gtggaaatac gatgatcacc cgcaccggct
     3001 ttgccgggta gttctgggcg agcgcggcga atggcgcgct caccgcggcc acgagagcga
     3061 gcgtcgcgag acgtgacaat cctttcatgc ttcctcctgt agttggtgca ctccggactc
     3121 gaggcggatt gtctcccagc ccccgggatg aagcaagccg cgctgccgcg ggcgccgcgg
     3181 caggcggttg cacggggccg gctgttgccg gccccggccg tctcggcggg cgtctgcccg
     3241 cccgatttcc gctaattcac cttcgtcccg agcgcgcgca gcatcttgcc gtaacgctcg
     3301 tggtccgatt gcaggcgctc ggcgaattgc ggcaccgtca tgtgcatggg atcaagcgtc
     3361 atggcggtga gtttcgaggc aacatcgggg tcactcacca ctttcttgaa ctcggcgttg
     3421 agcctgtcca cgatcggccg cggcgttttc gccggcgcga atgcgccgac ccacgcggta
     3481 aattcgtacc cggggacggt ttccgcaatg gccggcacat cgggaaactg gggactgcgc
     3541 ttctcggaag tcaccccgat cgcgcgcacg cggccggcct ttacatgcgt gaaaaccgaa
     3601 gcgatggtgg caatctggag ttgcgtttcc ccggacacga gcgcggtgcc ggcgggcccg
     3661 ccgcccttga acggcacgtg cagcatcttc gttcccgtca tcgacatcag gagttccacc
     3721 gtcagctgga tgtagctgcc gtggccggcc gacccgaaga cgatctcccc cgggcgcttc
     3781 ttcgccagca cgatcagctc ctttatcgag ttgaccggca gcgacgggtg tacgaccgcc
     3841 atggctacct gcctcgcgag cggcgtgatg ccgatgaaat cgcgcaatgt gtcgtactgc
     3901 agcttgctgc cgtacacatg cgcgttcgcg atgtgggtcg tggaatgcac catcagcgtg
     3961 tagccgtcgg ccgggctttg cgcgaccacg gtggcgccca gggaccccgc tcccccgccg
     4021 cggttgtcga tcaggaattg ctggcccatc gtttcggtca tcttctgaaa cacgatccgc
     4081 gccacgacgt cgttggaacc acccggcgga aacacgacga tcacacggac cggcttcgcc
     4141 gggtaagcct gcgcaaacgc agtcaatggg aaaaaacacg ccgccaggac gcacggcagg
     4201 accctgctga gaaagcactt catatatctc ctccttcgtt gtatgttttg tcgtgcgggc
     4261 gccgcagcct gcgagtggaa ccccgtcacg ccgccattcc accacatgtt ggactgggat
     4321 gtcgagccga cctttcccct cggtaatcaa caaaaggatt acttttcatg gcactaccgc
     4381 tggaaggcgt cagagtactc gatttgagca acatc
//
LOCUS       DBNGSG010000075         8022 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_24483, whole genome shotgun sequence.
ACCESSION   DBNGSG010000075 DBNGSG010000000
VERSION     DBNGSG010000075.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 8022)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8022)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8022
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_24483"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..452)
                     /locus_tag="AC158Q_01895"
     CDS             complement(72..452)
                     /locus_tag="AC158Q_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HIR3338550.1"
                     /translation="MSTFNRCIARLLIVCTLGMGMPVSSMAGIVATDRVHAGVERDQV
                     VNFLERADVRARMQGLGVDPDAARARVDAMSDDEVAALASRIDEMPAGGTDVLGALLL
                     VFIILLVTDILGLTKIFPFTRPVR"
     gene            complement(567..1394)
                     /locus_tag="AC158Q_01900"
     CDS             complement(567..1394)
                     /locus_tag="AC158Q_01900"
                     /inference="COORDINATES: protein motif:HMM:NF012730.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0034702 - monoatomic ion channel complex
                     [Evidence IEA];
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HIR3338551.1"
                     /translation="MKAFAKRIVEARWFEPWMIGLILFNGLLIGLETSKDFVEQYDGW
                     LHLGNDIILGIFIFEVVLKLAAVSPRFGLYFGNGWNLFDFTVVVLSLIPATGEFALVA
                     RLVRVLRVLRLVSTVPQLRLIVATLVRSIPSMGHVILLMSIIFYIYAITGYHLFHEHD
                     AEHWGTLGAALLTLFQMVTLEGWVEVMDTAMEVHPWSWIYFVTFVLIGTFVMLNLFIA
                     VVINNLDASKAAELEELRDPVTHEEVLDELKRTRDALASLQRRLEALPRAGTAQRRG"
     gene            complement(1499..3172)
                     /gene="ilvD"
                     /locus_tag="AC158Q_01905"
     CDS             complement(1499..3172)
                     /gene="ilvD"
                     /locus_tag="AC158Q_01905"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIR3338552.1"
                     /translation="MPLNRRSRLITHGVARSPNRAMLRAVGFGDGDFEKPIVGVANGH
                     STMNPCNAGIQPLVDRAMAALKQAGAMPQVFGVPTITDGIGMGTEAMKYSLVSREVIA
                     DVIETAVNGQAMDGVLVCGGCDKNMPGGLIAMVRMNVPGIYVYAGTIKPGKWKGKDLT
                     VVSAFEAVGAYAAGKMSEEDFIGVEKNACPSVGACGGMYTANTMSSSFEALGMSVLGS
                     SQMASPDPEKADSAAESARVLVNAIKQDIKPRDIVTKKSIENAVALIMATGGSTNAVL
                     HYLAIAHAAGVKWTIDDFERVRKKVPVLCDLKPSGRYVAVDFHRAGGVPQVLKMLLGH
                     GLLHGECLTISGRTMAEELKDVADEPRREQDVIRPWANPMYPHGHLAILKGNLAPEGC
                     VAKITGLKSPKITGPARVFDSENACMDTIMAKKIRAGDVIVIRYEGPKGGPGMQEMLA
                     PTAALIGQGLGESVGLITDGRFSGGTWGMVVGHVAPEAFEGGPIALVREGDSITIDAV
                     KRLIQLNVPSRELAARRKKWRQPRPRYTRGALAKYIKLVSTASKGAVTD"
     gene            3224..4021
                     /gene="lgt"
                     /locus_tag="AC158Q_01910"
     CDS             3224..4021
                     /gene="lgt"
                     /locus_tag="AC158Q_01910"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR3338553.1"
                     /translation="MLIHPQFDPVALQLGPLAVRWYGLMYLVGFALFWAAGRYRIART
                     PGAVWSAKDLEDALFYGILGVILGGRLGYVLFYKFSDYLGEPWRILYVWEGGMSFHGG
                     MLGVILALAWFARSRRQDWLAVTDFIAPLVPLGLAAGRLGNFINAELWGRPASVPWAV
                     VFPNVDGVPRHPSQLYEFALEGVVLFAVLWWFSMRRRPRGAVSGAFLLGYGVLRFGVE
                     FTREPDGFLGLLTLGLTMGQWLSLPMIAAGAAMMVWAYRPKTVDRGS"
     gene            complement(4089..4859)
                     /locus_tag="AC158Q_01915"
     CDS             complement(4089..4859)
                     /locus_tag="AC158Q_01915"
                     /inference="COORDINATES: protein motif:HMM:NF018928.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapC family protein"
                     /protein_id="HIR3338554.1"
                     /translation="MDIRPPYGYQEIVPLTKQHRVLLPEGRKLPLVFRALTALPLSYT
                     EFAVACRDYPIAFIGGEGNFVAMAVLGMENQQNLFVTPDGGWDASVYLPAYVRRYPFC
                     MTRVTVDGREQQERVACVEKRAINDKGEALYDAKGAPLPVWDERKKLLFEYEADLVRS
                     DEMSRTLAGLGLLETFTMQAVPKDGGPLALTGMYRVSEQKLAELAPDKLKELAQKGIL
                     ARVYAHLISLANFGRLLDRRAAQGKPAAAPARESRKPS"
     gene            5072..5147
                     /locus_tag="AC158Q_01920"
     tRNA            5072..5147
                     /locus_tag="AC158Q_01920"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5105..5107,aa:Ala,seq:cgc)
     gene            5255..5881
                     /locus_tag="AC158Q_01925"
     CDS             5255..5881
                     /locus_tag="AC158Q_01925"
                     /inference="COORDINATES: protein motif:HMM:NF013982.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HIR3338555.1"
                     /translation="MFSRLMPREGRFFDLFNAHAAQIVEGGRALARLFADFESSAVYA
                     EQIDVAERNADKITHETITLLHKTFITPIDRDQIHQLITTMDDVLDLVQDTAESIVLY
                     DIRRVSAEAQQLAQICQMTCDRVKAAVGLLANLRDPEAILKTCEEIDRLESDADRVMR
                     SAMSRLFREEPDVKQLIKLRSIYEYLELITDRCEDVANIIEGIVLEHS"
     gene            5895..6902
                     /locus_tag="AC158Q_01930"
     CDS             5895..6902
                     /locus_tag="AC158Q_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011402287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005315 - phosphate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HIR3338556.1"
                     /translation="MEPVPISLGVVIFLVLVALVFDFMNGFHDAANSIATVVSTRVLK
                     PYWAVIWAAFFNFVALFVFELKVAGTVGTGIVDTAIVDHYVVFGALAGAITWNIITWW
                     FGIPSSSSHALVGGIVGAAVAKAGTGALIASGLVKPVIFIILSPLLGFVLGSMLLLAV
                     SWICSRTTPAKVDKWFRRLQLVSASFYSLGHGGNDAQKTIGIIWMLLIAAGVSRPDEH
                     IPYWVVVACYVTIALGTLFGGWRIVKTMGMKITKLKPVDGFCAETGGGVTLFTTAALG
                     IPVSTTHTITGAIVGVGAARSFSAVRWGVAGMIVWAWVFTIPCSALIAAISWWLGRHL
                     F"
     gene            6914..>8022
                     /locus_tag="AC158Q_01935"
     CDS             6914..>8022
                     /locus_tag="AC158Q_01935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3338557.1"
                     /translation="MPAGPSTEHRALLADSVADFATRGTDIARVRKLRGTSGEYDRAV
                     WKKMAELGWLGILVPESHGGLGLGLTEMAIVAKGLARALVPEPLTATAVLAATALAAG
                     GNEALKREELPRLVSGACLSTLAWQEEAGHLDPAAAHTRAVTFEGGFKLNGLKRFIPG
                     AAQADAFLVSAQSENGLALLWLPRDTAGAQLEMDLLADGRGFGALGLRDALVPGGRVV
                     ATGAAAQEALARAFDHGSAIAGAELAGVMERALEMTLDYLRTRVQFGRPIGAFQALQH
                     RAVDLHIHKELANAVLDEALALLDRGPAPAARAALASRIKARCAEAAQIITREAIQLH
                     GAIGFTDEYDAGLYLKRALTLAAWLGHPNWHRRRYA"
BASE COUNT         1308 a         2635 c         2628 g         1451 t
ORIGIN      
        1 gagcgacagg acggcggaaa acaaaacccc cgcgatgacc gggattgccg gcaggcggcc
       61 cacgacgtcg cttaccgcac cggtctcgtg aacgggaaga tcttggtcaa cccgagaatg
      121 tcggtgacga gcagtatgat gaaaacgagc agcagcgcgc ccaggacatc ggtgccgccg
      181 gcgggcattt catcgatacg gctggcaagc gccgcgactt cgtcgtcgct catcgcgtca
      241 acgcgggccc gcgccgcatc ggggtcgacg ccgagcccct gcatcctggc gcgtacgtcg
      301 gcgcgctcca ggaaattcac gacctgatcg cgttcgacgc cggcatgaac gcggtccgtg
      361 gcgacgatgc cggccatgga tgacacgggc attcccattc caagagtaca gacgatgagc
      421 agacgtgcga tgcaccggtt aaaggtgctc atattgttac ctcctgtgac ttggaggagg
      481 caggaccgcc gacggttccc gcaaaggggc gcaaccggaa cgggctaacc gccagaatgt
      541 cgccttgcgg cgacaggaaa atttacctac ccgcggcgct gcgcggtccc ggcacggggc
      601 agcgcttcca gcctgcgctg cagggaggcg agcgcgtcgc gcgtgcgttt cagttcgtcg
      661 agcacttcct cgtgcgtgac cgggtcgcgc agctcctcga gttccgccgc cttgcttgca
      721 tcgaggttgt tgatcaccac cgcgataaac agattcagca tgacgaaagt cccgatcagc
      781 acgaaggtca cgaagtagat ccacgaccag gggtgcactt ccatcgccgt gtccatgacc
      841 tccacccagc cttccagcgt aaccatctgg aacagcgtca gcagggccgc ccccagcgtg
      901 ccccagtgtt ccgcgtcatg ctcgtggaac aggtgatagc cggtaatggc gtagatgtag
      961 aagatgatgc tcatcagcag gatgacgtgg cccatgctgg gaatcgagcg caccagggtg
     1021 gcgacgatca gccgcagctg cggcaccgtg gagaccagcc gcagcacgcg cagcacgcgc
     1081 accagccgcg ccaccagcgc gaactcgccg gtcgcgggaa taagcgacag cacgacgacc
     1141 gtgaaatcga agagattcca gccgttgccg aagtagagac cgaaacgcgg cgagaccgcg
     1201 gcaagcttca gcaccacttc gaagatgaag ataccgagga tgatgtcgtt gcccaggtgc
     1261 agccagccgt cgtattgctc gacgaaatcc ttcgacgtct ccaggccgat cagcaagccg
     1321 ttgaagagga tcaacccgat catccacggc tcgaaccagc gcgcttcgac gatacgcttg
     1381 gcgaaagctt tcatgtcagt gtctgtgtct gcgaaaatgt tggatcgcgt gatgaagggg
     1441 gtgcgggggc aacgggaagg ccccgaggat ctgccggaag gggcccgttc cccgccgctc
     1501 aatcggtgac tgcgcccttg gaggcggtcg acaccagctt gatgtacttc gccagcgccc
     1561 cgcgcgtgta gcgcggcctg ggctgacgcc acttctttct gcgggcggca agttccctgg
     1621 acggcacgtt caactggatc aggcgcttta ccgcgtcgat cgtgatggaa tcgccctcgc
     1681 gcacgagagc aatcgggccg ccctcgaacg cttccggcgc aacgtggccg accaccatgc
     1741 cccaggtgcc gccggaaaac cgaccgtcgg taatgaggcc gacggattcg ccaaggccct
     1801 ggccgatcag cgccgcggtc ggcgccagca tttcctgcat gccggggccg cccttcgggc
     1861 cctcgtagcg gattacgatc acgtcgccag cgcggatctt cttcgccatg atcgtgtcca
     1921 tgcacgcgtt ctcggagtcg aacacgcgcg ccggcccggt gatcttcggc gatttcagcc
     1981 cggtgatctt ggccacgcac ccttccggcg cgaggttgcc cttgaggatg gcgagatggc
     2041 cgtgcgggta catcgggttg gcccacgggc ggatcacgtc ctgctcgcgg cgcggctcgt
     2101 cggccacgtc tttcaactcc tccgccatcg tgcgaccgct gatcgtcagg cattcgccgt
     2161 ggagcaggcc gtgcccgagc agcatcttca gcacctgcgg cacgccgccc gcgcggtgga
     2221 agtccaccgc cacgtagcgc ccggagggct tcaggtcgca cagcaccgga actttcttgc
     2281 gcacgcgctc gaagtcgtcg atcgtccact tcactcccgc ggcatgcgcg atcgcgaggt
     2341 agtgcagcac cgcattggtc gagccgccgg tcgccatgat gagcgcgacc gcgttctcga
     2401 tgctcttctt cgtgacgatg tcgcgcggct tgatgtcctg cttgatggcg ttgaccagca
     2461 cgcgcgccga ctcggccgcg gaatccgcct tctccgggtc gggcgaggcc atctgcgacg
     2521 aacccagtac gctcatgccg agcgcctcga acgaggagga catggtgttg gcggtgtaca
     2581 tgccgccgca cgcgccgaca ctggggcagg cattcttctc gacgccgatg aaatcctcct
     2641 cgctcatctt gccggcggcg taggcgccca ccgcctcgaa cgcggagacc acggtgagat
     2701 ccttgccttt ccatttgccc ggcttgatgg tgccggcata gacgtaaatc ccgggaacgt
     2761 tcatgcgcac catcgcgatc aggccgcccg gcatgttctt gtcgcagccg ccgcacacca
     2821 gcacgccgtc catcgcctgg ccgttgaccg ccgtctcgat gacgtcggca atcacttcgc
     2881 gcgagaccag cgagtacttc atcgcctcgg tgcccatgcc gatgccgtcg gtgatggtcg
     2941 gcaccccgaa cacctgcggc atcgcgccgg cctgcttcag cgccgccatc gctcggtcca
     3001 ccagcggctg aatgccggca ttgcagggat tcatcgtcga atgcccgttg gccacgccga
     3061 cgatgggttt ctcgaaatcg ccgtcgccga agccgaccgc gcgcagcatc gcccggttgg
     3121 gggagcgcgc gacgccgtgg gtgatgagac ggctgcgtcg attgagcggc atgggggata
     3181 ctcgcggcgg ctataatgaa gggcgccatt ttagcgcatc cccatgctga tccatccgca
     3241 attcgatccc gtcgcgctcc agttgggtcc actcgcagtg cgctggtacg gcttgatgta
     3301 cctcgttggc ttcgccctgt tctgggccgc cggccgttac cgcatcgcgc gcacgcccgg
     3361 cgccgtgtgg tcggcaaagg acctcgagga tgcgctgttc tacgggatac tgggcgtgat
     3421 cctcggcggg cggctgggct acgtgctgtt ctacaaattc tccgactatc tgggcgagcc
     3481 gtggcgcatc ctctacgtct gggaaggcgg catgtcgttt cacggcggca tgctgggcgt
     3541 gatcctcgcc ctggcgtggt tcgcgcgcag ccgccggcag gactggctcg cggtgacgga
     3601 cttcatcgcg ccgctcgtcc cgctggggct cgccgcgggg cggctcggca acttcatcaa
     3661 cgcggaactg tggggacggc cggcctcggt gccgtgggcg gtcgtgtttc ccaacgtcga
     3721 cggcgtgccg cgccacccct cgcagctcta cgagttcgcg ctggaagggg tcgtgctgtt
     3781 cgcggtgctg tggtggttct cgatgcgccg gcgcccgcgc ggcgcggtgt cgggcgcgtt
     3841 cctgctggga tacggcgtgc tgcgcttcgg ggtggagttc acgcgcgagc ccgacggttt
     3901 tctcggactg ctcacgctgg ggctcacgat ggggcagtgg ttgtcactgc cgatgattgc
     3961 cgccggggcg gcgatgatgg tttgggccta taggccgaaa accgtggatc gtggatcgtg
     4021 atacgtgggt cgacttggct gatcagttcc aatcgatccg caacccgcga cccgcgtttt
     4081 tcagaatctt aactgggttt cctgctctcg cgtgcgggcg cggcggccgg cttgccctgc
     4141 gcggcgcggc ggtcgagcag ccggccaaaa ttcgcaagtg aaatcaggtg cgcgtagacg
     4201 cgcgccagta tgcccttctg cgcgagctcc ttcagcttgt ccggagcgag ctcggcgagc
     4261 ttctgttcgg acacgcgata catgccggtc agcgcgagcg gcccgccgtc cttgggcacc
     4321 gcctgcatgg tgaaggtctc cagcagcccg agcccggcga gcgtgcgtga catctcgtcg
     4381 ctgcgcacga gatcggcctc gtattcgaac agcagcttct tgcgctcgtc ccacaccggc
     4441 agcggcgcgc ccttggcatc gtagagcgct tcgcccttgt cgttgatcgc gcgcttctcc
     4501 acgcaggcga cgcgttcctg ctgctcgcgc ccgtccaccg tcacgcgggt catgcagaac
     4561 gggtagcggc gcacgtaggc cgggaggtag accgaggcgt cccatccccc gtccggcgtc
     4621 acgaacaagt tctgctggtt ttccatcccc agcaccgcca tcgcgacgaa atttccctcg
     4681 ccgccgatga aggcgatcgg gtagtcgcgg cacgccacgg cgaactcggt ataggagagc
     4741 ggcagcgccg tcagggcgcg aaacacgagc gggagcttgc gcccctcggg cagcagcacg
     4801 cggtgctgct tggtgagcgg gacgatctcc tgataaccat agggcggtcg gatatccatg
     4861 gcaacctcgc aggtgacgcg cgttgagcgg cgagccacag ggggagcgtg tcccccgcgc
     4921 cttgtccgtc cgctcgaacg ctcggagtta aacgggcgtc gcgcgcccgc tttaacggtg
     4981 attatatagt tccgcaaccg gcggccaccg cggccaagat tgtcgtttcc cgccaagccg
     5041 ttgtagaatc ccgccctcgc tgcaaaaccg tggggacgta gctcagctgg gagagcgtcg
     5101 cgttcgcaat gcgaaggtcg ggagttcgat cctcctcgtc tccaccacac atgccgggcc
     5161 ggatttccat ccgacttttt tgttgcccaa gtccggcgcc ggcgacaccg gggcttctgg
     5221 cagaatagcg gcttccgctt cccgaggcca caccatgttc agccgattga tgccgcgtga
     5281 aggccggttc ttcgacctgt tcaacgccca cgcggcgcag atcgtcgagg gcggacgcgc
     5341 gctggcgcgg ctcttcgccg acttcgagtc ttccgcggta tacgcggagc agatagacgt
     5401 cgccgagcgc aacgccgaca agatcacgca cgagaccatc acgctcctgc acaagacgtt
     5461 catcaccccg atcgaccgcg accagatcca ccagctcatc acgaccatgg atgatgtgct
     5521 cgacctcgtc caggacacgg ccgagtcgat cgtgctctac gatatccggc gcgtcagcgc
     5581 cgaggcgcag cagctcgccc agatctgcca gatgacctgc gaccgggtga aggcggccgt
     5641 tggcctgctc gcgaacctgc gcgacccgga ggcgatactc aagacgtgcg aggaaatcga
     5701 ccgcctggag tccgatgccg accgggtgat gcgatccgcg atgtcgcggc tgttccgcga
     5761 ggagcccgat gtcaagcagt tgatcaagct gcgctcgatc tacgagtatc tcgagctgat
     5821 caccgaccgc tgcgaggacg tggccaatat catcgaaggc atcgtgctcg agcactcata
     5881 gccaggctgc cttgatggaa cccgtcccga tcagccttgg cgtcgtcata ttcctcgtgc
     5941 tcgtcgccct cgttttcgat ttcatgaacg ggtttcacga cgcggcgaac tcgatcgcca
     6001 cggtggtttc gacccgcgtg ctcaagccgt actgggcggt gatctgggcg gcgttcttca
     6061 acttcgtggc gttgttcgtg ttcgagctca aggtcgcggg caccgtcggt accggcatcg
     6121 ttgacacggc aatcgtcgat cattacgtgg tgttcggcgc cctcgcggga gcgattacct
     6181 ggaacataat cacctggtgg ttcggcatcc cgtcgagttc ttcgcatgcc ctggtcggcg
     6241 gcatagtcgg cgcggccgtt gccaaggcgg gtaccggagc gctcatagcg agcggcctgg
     6301 tgaaaccggt catcttcatc atcctgtcac cgctgctcgg gttcgtgctc ggttccatgc
     6361 tgctgctggc ggtttcgtgg atctgttcgc gcacgacccc cgcgaaagtc gacaaatggt
     6421 tccggcgcct gcaactcgtc tcggcgtcgt tctacagtct gggacatggc ggcaatgatg
     6481 cgcagaagac catcggcatc atctggatgc tgttgattgc agcgggcgtg tcccgccccg
     6541 acgagcacat tccttactgg gtagtggtcg cgtgctatgt caccatcgca ctcggcacgc
     6601 tgttcggcgg ctggcgtatc gtcaagacga tggggatgaa gatcaccaaa ctcaagccgg
     6661 tggacggctt ttgcgccgag accggcggcg gcgtgacctt gtttacaacg gcggcgctgg
     6721 gtattccggt atcgaccacg cataccatta ccggggcgat tgtcggcgtg ggcgcggccc
     6781 gctcgttctc cgcggtgcgt tggggggtgg ccggcatgat cgtctgggcg tgggtattca
     6841 ccattccctg ctccgccctc atcgcggcga tatcctggtg gctgggacgc cacctgtttt
     6901 gatcgggcgc acgatgccgg ccggcccctc gacagagcac cgcgcgctgc tggccgacag
     6961 cgtggccgat ttcgccacgc gcggcaccga catcgcgcgt gtgcgcaagc ttcgcggcac
     7021 gagcggcgag tacgaccgcg ccgtgtggaa aaagatggcg gaactcggct ggctcggcat
     7081 cctggtgccg gagagccacg gcgggctcgg gctgggtctc accgagatgg cgatcgtcgc
     7141 gaaagggctc gcgcgcgccc tggtccccga gccgctcacc gccacggcgg tgctggcggc
     7201 gaccgcgctc gcggccggcg ggaacgaggc gctgaagcgc gaggagttgc cgcgcctcgt
     7261 ttccggcgca tgcctgtcca cgctggcctg gcaggaagag gcaggtcacc tggaccccgc
     7321 cgcagcgcat acccgggcgg ttacgttcga gggtggtttc aaactgaacg gcctcaagcg
     7381 cttcattccc ggggcggcgc aggccgatgc tttcttggtc agcgcacaat cggaaaacgg
     7441 tcttgcgctg ctgtggctgc cgcgcgacac cgcgggcgcg cagctcgaaa tggatctcct
     7501 cgccgacggc cgcggcttcg gcgcgctcgg cctgcgcgac gccctggtgc ccgggggccg
     7561 cgtggtggca accggcgccg ccgcgcagga agcgcttgcc cgcgcgttcg atcatggcag
     7621 cgcgattgca ggcgccgagc tcgcgggtgt gatggagcgg gcgctcgaaa tgaccctcga
     7681 ctacctcagg acgcgcgtgc agttcggaag accgatcggg gccttccagg cgctgcagca
     7741 ccgcgcggtg gatctccata tacacaagga gctggccaac gccgtgctcg atgaggcgct
     7801 tgccctgctc gaccgcgggc ccgcccccgc cgcgcgcgct gcgctggcga gccgtatcaa
     7861 ggcgcgctgc gcggaggctg cacagatcat tacgcgcgag gcgatccagc tccacggcgc
     7921 gatcggtttc accgacgagt acgacgcggg gctatatttg aagcgcgcgc tcacgctggc
     7981 ggcctggctc gggcacccga actggcatcg ccgccgctac gc
//
LOCUS       DBNGSG010000076         3446 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_873878, whole genome shotgun sequence.
ACCESSION   DBNGSG010000076 DBNGSG010000000
VERSION     DBNGSG010000076.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_873878"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..140
                     /locus_tag="AC158Q_01940"
     CDS             6..140
                     /locus_tag="AC158Q_01940"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF012496.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HIR3338558.1"
                     /translation="MKAWKGAASNVAAAQSALLHRSRMNSLACSGRYSSELERQALAA
                     "
     gene            220..1314
                     /locus_tag="AC158Q_01945"
     CDS             220..1314
                     /locus_tag="AC158Q_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CobW family GTP-binding protein"
                     /protein_id="HIR3338559.1"
                     /translation="MTAAIVDRPPVTLITGYLGSGKTTLLNRLLAGPDMARAAVIINE
                     FGAIGLDHLLVATPAENTVLLANGCICCTVRGDLVDTLRDLGGRRRDGSLPSFDRVLI
                     ETTGLADPVPLLQTLIADEVVARLYRLDGVVTLVDAVHGAGTLDEHAEAVKQAALADR
                     MLIAKCDLAGEKTIAALEGRLARINPGARRLRMVCGDIAPREIFGAALGGADAAGRVA
                     RWLDYEAYAAADTGPGQDHHHPGGSIRAHSFRIDRPVTRAGLMTWLTLLASMRGASLL
                     RVKGLLNVEGAPFAVHAVQTVIDEPVELPRWPDGERRSRLVFITRGLAREDIKRTLAA
                     LEVDAGRRSPRGTLDPQAYARFVAAAQGFR"
     gene            1352..2698
                     /locus_tag="AC158Q_01950"
     CDS             1352..2698
                     /locus_tag="AC158Q_01950"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase family protein"
                     /protein_id="HIR3338560.1"
                     /translation="MEIKAPEPKGPVKYVRAGLLIDGTGKKPVREPVIELEGRRVKRV
                     GTRASLKVPPRAEVIDCGAFTVMPGMIDAHLHTMMFNCLTFHNYRVAQWEITPELQQM
                     YGLFHAQLCFDMGFTTLRDLGLNSSRGLLTAELCAVREAINAGIVEGPRMLIGGFTTI
                     TGSHLDLIQPRAMQRLGFQTADGPWELRKLARTNLLAGCDIVKTCATGGGGTDKEEPD
                     IRNMTQEEINAIVDEAHAFHKVAAVHCFTPQGQRMALEAGADTIEHMVFSEAETTDRI
                     AKAGVWMTPTLLHRTDHAIQTRKDQGTSQFVLNKMKQIQPECFRTFQRMHKAGVNIAM
                     GTDMGYDPEMGTNARELEIYVDLGMKPMDALLTATRNAARALKLDHELGTIEPGKLAD
                     LIVVDGDPSKNIRCLQEKKNIKMVMKEGRVYADRRPGKNKNVVNVKPGEWKIIDYL"
     gene            2770..3252
                     /locus_tag="AC158Q_01955"
     CDS             2770..3252
                     /locus_tag="AC158Q_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3338561.1"
                     /translation="MAARKRALKKAKTRARTKTAGAVKGGNGRYQFNLAQVKDVAAGT
                     GYSTSHGGVVEGERMLVGYIHKERGTGSRMHTHRNEQFNLVVRGTLVGSVNGRKIFAP
                     AGTLIYIPANAPHTLVASPDDEDVIFIAIKDLSQGIIGRAVDGTMAGPHYEKGFGPRD
                     "
BASE COUNT          636 a         1151 c         1153 g          506 t
ORIGIN      
        1 ccgcgctgaa agcgtggaaa ggcgccgcct ccaacgtcgc cgccgcgcag tcggcgctgc
       61 tgcaccgctc gcgcatgaac agccttgcct gctccggccg ctattcgtcc gagctcgagc
      121 gccaggcgct cgcggcttag ggcttcggaa ccgccaagcc cgccaagatt cgatccgcag
      181 agcttcagcg ccgccgcgat ttcacgagct atcgccaaca tgaccgcggc aatagttgat
      241 cgtccgccgg tcaccctcat caccggctac ctgggcagcg gcaagacgac gctgttgaac
      301 cgcctgctcg ccggcccgga catggcgcgg gccgccgtca tcatcaacga gttcggcgcg
      361 atcgggctcg accacctgct ggtcgcgacg cccgccgaga acacggtgct gctcgcaaac
      421 ggctgcatct gctgcaccgt gcgcggcgat ctcgtggaca cgctgcgcga cctcggcggc
      481 cggcgccgcg acggctcgct gccgtccttc gaccgggtgt tgatcgaaac caccgggctc
      541 gccgaccccg ttcctctgct gcagaccctg attgccgacg aggtcgtggc gcgcctctac
      601 cggctggacg gcgtggtgac gctggtggac gcggtgcacg gcgcgggcac tctcgacgag
      661 catgccgaag ccgtcaagca ggccgcgctc gccgaccgca tgctgatcgc caagtgcgat
      721 ctcgccggcg agaagaccat cgccgcactg gaaggcaggc ttgcccgcat caatcccggc
      781 gcgcggcggc tgaggatggt gtgcggcgat atcgccccgc gtgagatctt cggcgcggcg
      841 ctcggcggag cggatgccgc ggggcgtgtc gcccgctggc tcgattacga ggcttacgcg
      901 gcagcagata ccggtcccgg acaggaccat caccatcccg gcgggtccat ccgcgcgcac
      961 agctttcgca tcgaccggcc cgtgacccgt gccgggctga tgacctggct caccctgctt
     1021 gcgtcgatgc gcggcgccag cttgctgcgc gtcaaggggc tgctcaacgt cgaaggcgcg
     1081 ccgtttgccg tgcacgcggt gcaaaccgtg atcgacgagc cggtcgaact tccgcgctgg
     1141 cccgatggcg agcgccgctc gcggcttgtc ttcatcacgc gggggctggc acgcgaagac
     1201 atcaaacgca cgctcgccgc gctcgaggtg gacgcgggcc gccgctcgcc tcggggcacg
     1261 ctcgatcccc aggcttacgc gcgcttcgtc gcggcggcgc agggctttcg ctgatcgctc
     1321 aaatcgctat ccttagagaa aggcataccg catggagatc aaggcgccgg aacccaaggg
     1381 cccggtcaaa tacgtcaggg caggcctgct gatcgacggc accgggaaaa agccggtcag
     1441 ggagccggtg atcgaactgg aggggcgccg cgtgaagcgc gtcgggaccc gcgcgagcct
     1501 caaggtcccg ccgcgcgcgg aggtgatcga ctgcggcgcc ttcaccgtga tgcccggcat
     1561 gatcgacgcc cacctgcaca ccatgatgtt caactgcctg accttccata actaccgcgt
     1621 cgcgcaatgg gagatcacgc cggagctgca gcagatgtac gggctgttcc atgcccagct
     1681 ctgcttcgac atgggcttca ccacgctgcg cgacctcggg ctcaattcga gccgggggct
     1741 gctgaccgcg gagctgtgcg ccgtgcgcga ggcgatcaac gccggcatcg tggaggggcc
     1801 gcgcatgctg atcggcggct tcaccaccat caccggctcg cacctcgatt tgatccagcc
     1861 gcgcgcgatg cagcggctgg gcttccagac cgccgacggg ccgtgggagc tgcgcaagct
     1921 cgcgcgcacc aatctgctcg ccggctgcga catcgtgaag acctgcgcga ccggcggcgg
     1981 cggcaccgac aaggaggagc ccgacatccg caacatgacg caggaggaga tcaacgcgat
     2041 cgtggacgag gcgcacgcct ttcacaaggt cgccgcggtg cactgcttca cgccccaggg
     2101 ccagcgcatg gcgctcgagg cgggcgccga caccatcgag cacatggtgt tctccgaggc
     2161 ggagacgacc gaccgcatcg cgaaggcggg cgtgtggatg acgccgacgc tgcttcaccg
     2221 caccgaccac gcgatccaga cacgcaagga ccagggcacc tcccagttcg tgctgaacaa
     2281 gatgaagcag atccagcccg agtgcttccg caccttccag cgcatgcaca aggccggcgt
     2341 caacatcgcg atgggcaccg acatgggcta cgatccggaa atgggcacca acgcgcgcga
     2401 gctcgagatc tacgtggacc tcggcatgaa gccgatggac gcgctgctca ccgccacgcg
     2461 caacgctgcg cgcgcgctga agctcgacca cgagctgggg acgatcgagc cgggcaagct
     2521 cgccgatctc atcgtggtgg acggcgaccc ctcgaagaac atccgctgcc tgcaggagaa
     2581 gaagaacatc aagatggtga tgaaggaagg ccgggtttac gccgatcgcc ggccgggaaa
     2641 gaacaagaac gtggtcaacg tgaagccggg ggaatggaag atcattgatt acctgtagac
     2701 tgcgtgaatc gtgattcgtg agtcgtgatt cgacccggga acacgcagcc gcttgattgg
     2761 agaatccgca tggcagcgag gaagcgcgcg ctgaagaagg cgaagaccag ggcccgcacc
     2821 aagaccgcgg gtgcggtgaa aggcggcaac ggcagatatc aattcaacct ggcgcaagtg
     2881 aaggacgtcg cggcgggcac cggctactcg acctcgcacg gcggcgtggt cgagggcgag
     2941 cgcatgctgg tgggctatat ccacaaggag cgcggcaccg gctcgcgcat gcacacccac
     3001 cggaacgagc aattcaacct cgtggtgcgc ggcaccctcg tgggatcggt caacggcagg
     3061 aaaatctttg ctccggccgg cacgctgatc tacattcccg ccaacgcccc gcataccctg
     3121 gtggcctcgc cggacgacga ggacgtgatc ttcatcgcga tcaaggacct gtcgcagggc
     3181 attatcgggc gcgcggtcga cggcaccatg gccgggccgc actacgagaa gggcttcggc
     3241 ccgcgggact gaacgccgat agaattcgat catagccggc tgcaatccga agttcgccga
     3301 agcgttcagg ggtgctgggc cgggcaacgc ggagccggcg taaaggtgga gtacttatgc
     3361 ggaggaggca gagatgaaac accgaggatt cacgcaggca ctgttggtcg cagcagcgtt
     3421 caccgccgtg ccggccgtca tggcgc
//
LOCUS       DBNGSG010000077         1965 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3930825, whole genome shotgun sequence.
ACCESSION   DBNGSG010000077 DBNGSG010000000
VERSION     DBNGSG010000077.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3930825"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="AC158Q_01960"
     CDS             complement(<1..659)
                     /locus_tag="AC158Q_01960"
                     /inference="COORDINATES: protein motif:HMM:NF019352.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HIR3338562.1"
                     /translation="MNADERRFGRRRFLQGIAAGSLALAGRPPALSAGLAHAAMPPAG
                     ATAGFKPDIELELNAIAGEIRVLPGNATRLWRFTGRVVRGNPQALTFLDGSRHIPVIH
                     ARRGQKLRITFTNQLPEDSIVHWHGLHIVQRMDGHPMYAIGSGRKFVYEFVVDNRAGT
                     HWFHPHPHERTGLQIYFGLAGLFLVADDEEDAARLPRDEFDLPLVIQDRRFNAANQLV
                     YL"
     gene            complement(666..1337)
                     /locus_tag="AC158Q_01965"
     CDS             complement(666..1337)
                     /locus_tag="AC158Q_01965"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3338563.1"
                     /translation="MNRISWLKALVALSACAGLLAGPSAAQSPHTHRHSFGDAEKWAQ
                     IFDDPKRDAWQKPHEVIQALALKPDAVVADIGSGTGYFSARIANMTPRGRVYGVDTEP
                     DMVKYLSERAKREGLKNVTAVQAKPGDPRLPEKADMVILVDVYHHVENREQYFRQLQN
                     SLKPGGRLAVIDFRMEAPDGPPKSARIAPEQVKAELQRAGYALDREHTFLPNQYFLVF
                     RPGDR"
     gene            complement(1433..1882)
                     /locus_tag="AC158Q_01970"
     CDS             complement(1433..1882)
                     /locus_tag="AC158Q_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338564.1"
                     /translation="MKKSHQFAVGVIASLGLGIAVATAYANPGQMGGGMEHGKGGMQH
                     GAQGGMQHGAQGGMQHGAQSGMQHGGMGGGAGHGAGQQFMTPEERTALQEKMRNARTP
                     EERQQIATAARAEMRKRATEKGITLPEGRGPRTGTTPQAPATTEHAH"
BASE COUNT          294 a          635 c          652 g          384 t
ORIGIN      
        1 aggtagacga gttggttggc ggcattgaat cgccggtcct ggatgaccag cggcaggtcg
       61 aattcgtccc gcggcagccg cgcggcatcc tcctcgtcgt ccgcgacgag gaacagtccg
      121 gccaggccga aatagatctg caggccggtg cgctcgtgcg ggtgcgggtg gaaccagtgg
      181 gtgccggcgc gattgtccac cacgaattcg tagacaaatt tcctgccgct gccgatcgcg
      241 tacatgggat gcccgtccat gcgctgcacg atatgcaggc cgtgccagtg cacgatcgag
      301 tcctcgggaa gctggttcgt gaaggtgatg cgcagctttt gcccgcggcg cgcgtggata
      361 accggaatgt gccgcgagcc gtcgaggaag gtcagcgcct ggggattgcc gcgcaccacg
      421 cggccggtaa agcgccacag gcgagtcgcg ttgccgggca gaacccggat ttcgccagca
      481 atggcattca gctccagctc gatgtccggc ttgaagccgg cagtcgcccc ggccggcggc
      541 atggcggcat gcgccagtcc ggcggacaag gccggtgggc ggcccgccag cgcgaggctc
      601 cctgctgcaa taccctgcag gaaccggcgg cggccgaatc ggcgttcatc ggcgttcatc
      661 ggcggctaac ggtcgcccgg ccggaacacc aggaaatact ggttcggcag gaaggtgtgt
      721 tcccggtcca gcgcgtaacc ggcgcgctgg agctcggctt tcacctgctc gggcgcgatg
      781 cgtgcgctct tcggcgggcc gtcgggagcc tccatgcgga aatcgatgac ggcgaggcgg
      841 ccgccgggtt tcagcgaatt ctgcaactgg cggaaatact gctcgcggtt ctccacgtgg
      901 tggtagacgt cgacgagtat gaccatgtcg gctttttccg gcaggcgcgg atcgcccggc
      961 ttcgcctgta ccgccgtgac gttcttcagt ccctcacgct ttgcgcgctc ggacaggtac
     1021 ttcaccatgt cgggctcggt atccacgccg tagacccggc ccctgggcgt catgttcgcg
     1081 atgcgcgccg agaaataccc ggtgccggat ccgatgtcgg cgaccaccgc gtcgggcttg
     1141 agcgccagcg cctggattac ctcgtgaggc ttctgccacg cgtcgcgctt cgggtcgtca
     1201 aagatctgag cccatttttc agcgtcgccg aaactgtgcc ggtgagtgtg cggggattgt
     1261 gcagctgatg ggcctgcaag caggccggca caggcggaca gtgccaccaa tgccttgagc
     1321 catgaaattc ggttcattgt ttcgtctcct gtgggtgccg cgcctatcag gcatcccgag
     1381 agtatgaaag cgaagcgggg cgggtttccc cgccccggta cggtagctgc gatcagtgcg
     1441 cgtgctcggt cgtggccggc gcctgggggg tggtcccggt acgcggaccg cggccctcgg
     1501 gcagcgtgat gcctttttcc gttgcgcgct tccgcatctc ggcgcgagcc gccgtggcga
     1561 tctgctggcg ttcctcgggc gtcctggcgt tgcgcatttt ctcctgcagc gccgtccgct
     1621 cttccggcgt catgaactgt tgcccggcgc catgtcctgc gccgccgccc ataccgccgt
     1681 gttgcatgcc actctgcgcg ccgtgctgca tgccaccttg ggcgccgtgc tgcatgccac
     1741 cctgcgcgcc gtgctgcatg ccacctttgc cgtgctccat cccgccgccc atctgacccg
     1801 ggttggcgta ggcagtggct accgcaatgc caaggcccaa tgatgcgata acacccactg
     1861 cgaattggtg agactttttc atgactgttt cctttatgaa gattgaatgt cgatacctcg
     1921 atcgcccgtc gccgtctccg aagcctggac gcaacgacga tcgct
//
LOCUS       DBNGSG010000078         4593 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3931026, whole genome shotgun sequence.
ACCESSION   DBNGSG010000078 DBNGSG010000000
VERSION     DBNGSG010000078.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4593)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3931026"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1418)
                     /locus_tag="AC158Q_01975"
     CDS             complement(<1..1418)
                     /locus_tag="AC158Q_01975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR3338565.1"
                     /translation="MDDNVLYTHAEASLSGALGSRSSRPGGAEGDKRIAPVEFPVFGE
                     RQMRNFEASLRPMRIFDRETLKYLAVNDAALKLYGYTRDEFLALNLLDTRHPDEHASV
                     LAMLASQTDYIAHWGGRRHVTKSGDVLEVETITQDILFNGRRARLSLTIDITGRLRMQ
                     ELLWRRQQEFETLAENLPDLVARYDRNRRFLYANSAIERTFGIERGQLIGRTQREAGF
                     PEELAVLYDDSLAGVFATGQPHKVEFRIDCPGGERLFEAQHVAEYDAAGGIGSVLCVA
                     RDITSHKRAERSLLETQQHLQVAVRGGSVGLYDWNVKTGAVYFSPEWKKQLGYEDHEL
                     PNDFRAWESRIHPDDRVRISSATQDYLANPAIDYQQEYRLRHKGGSYRWMLSRASAHL
                     GAGGGVERMFGCHVDITDQKRAEAERLENAVRQRDALVREVHHRIKNSLQGVVGLLGQ
                     KIRKNPAIASEIEETIGQLQSVA"
     gene            complement(1488..>4593)
                     /locus_tag="AC158Q_01980"
     CDS             complement(1488..>4593)
                     /locus_tag="AC158Q_01980"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR3338566.1"
                     /translation="TVSLVRDASGKPDYFIRIVEDITERKHADEAIASERALLRTLVD
                     AVPDRIYVKDREGRFLLQNMANARAHGARSPEELLGKTIFDIMPRAAAERIEAEDRAI
                     LLSETPVIERERSSVDGAGNPRWVAASKVPLRDATGNVIGIVGVNRDITERKISAQRR
                     EVEHAVTVVLAEAPTLEAAMPHLIRTMCDGMGWAYGARWLWNKEENTLVRGEYWSDFV
                     PDFTGVDETPWLKLRFQAPGGLLRRAWFEKEPTWIEDVQREESFNRKETALKFGLHSA
                     YSFPIIAGTEVIGVMEFFGRQPRQPDSMLLQISGSIGRQIGQFVQRKQAESALKESEQ
                     QFRQLAENIPQVFWITDVEQHEAIYVSPAWEEITGRPLDQIHRNARSWLDSVHEEDRA
                     RVKNARKSATGGGYDESFRIVRPDGSVRWVHDRAFPVQDDTGAVYRIAGIAEDVTERK
                     LAETQLMQLAHYDVLTNLPNRVLFYDRLKQALAQARRNQWNVGVLFIDVDRFKNVNDT
                     LGHAMGDKLLQQVSERLTGAVRAGDTVGRLGGDEFSIVLSNLTSAQDANLVAQKIMAG
                     FNEPFKLDSAEIYVTASIGITLYPDDSVEQDTLIKNADAAMYRAKELGRNSFQFYMPE
                     MNTRAQALLSMESSLRRALDRGEFILHYQPKASAATGEITGVEALLRWQSPERGLISP
                     AEFIPVLEETGLIVPVGDWVIKAACAQVKAWQRSGVEPVPVAVNLSARQFQSPKLGAS
                     IVAAIEESGIEPRFLELEITESSLMQNADGAISTLQYLKSCGINVSIDDFGTGYSSLA
                     YLKRFPLDALKIDRSFVRDITSDPDDATITRAVISMAHSLGLKVVAEGVETESQFEFL
                     AENGCDEIQGYYFSRPLTADDCGSWLGEKRRLVRLSQPAEFAPTVLLVDDDDDTLALL
                     KRSLAKDGYQLLTARNAHEALGLLGRHRVDLVISDQNMPGVQGVEFLQRVKRLHPRTM
                     RMMTSGDADVRIVTDAINKGEIFRFLPKGMKEEQLRSDVREAMQARQRFAPERSIPPG
                     SLGK"
BASE COUNT          728 a         1554 c         1402 g          909 t
ORIGIN      
        1 gcgaccgact gcagttgccc aatcgtttcc tcgatctcgg aggcgatcgc gggattcttc
       61 ctgatcttct ggccaagcag tccaacgacg ccctgcaggc tgttcttgat ccggtgatgg
      121 acttcccgca ccagcgcgtc gcgctggcgc acggcgttct ccagccgctc ggcttcagcc
      181 cgtttctggt ccgtgatatc cacgtggcag ccgaacatcc gctctacacc gccgcccgcg
      241 cccagatggg ccgaggccct cgacagcatc cagcgatatg atccgccctt gtgccgaagc
      301 cggtactcct gctggtaatc gatcgccggg tttgccaggt aatcctgcgt cgccgaagaa
      361 atgcgtaccc ggtcatcggg atgtatccgg ctttcccatg ccctgaagtc gttgggcaac
      421 tcgtgatcct cgtagcccag ctgttttttc cactcggggg aaaaatagac cgcccccgtt
      481 ttgacgttcc agtcgtagag cccgaccgac ccgccgcgga cggccacctg gaggtgctgc
      541 tgggtctcga gcagcgagcg ctccgcgcgc ttgtggctcg tgatatcgcg tgcgacgcag
      601 agcacgctgc cgatgcctcc ggctgcgtca tattcggcga cgtgttgcgc ttcgaatagt
      661 ctctcgccgc ctggacaatc tatccggaat tcgaccttgt gcggctgacc agtggcgaag
      721 acgccggcga ggctgtcgtc gtacagcaca gcaagttcct cgggaaagcc ggcttcgcgt
      781 tgcgttctgc cgatgagctg tcctcgctcg atcccgaaag ttctttcaat cgccgagttg
      841 gcgtagagaa atcgccggtt ccggtcgtaa cgcgcgacca ggtcgggcag gttctcggcc
      901 agcgtctcga attcctgctg gcgccgccac agcaattcct gcatgcgcag ccgcccggtg
      961 atgtctatgg tcaggctcag ccgcgccctg cgtccattga acaggatgtc ttgcgtgatg
     1021 gtttcgacct cgagaacgtc gccggatttg gtgacgtgcc gcctgccccc ccagtgggcg
     1081 atgtagtccg tctggctggc gagcatggcg agcacgctcg cgtgctcgtc cggatgacgg
     1141 gtatcgagca ggttgagagc aaggaactcg tcgcgcgtat agccgtacag cttcagggcc
     1201 gcgtcgttga ccgcgagata cttgagcgtt tcccggtcga atatccgcat cgggcgcagg
     1261 ctcgcctcga agttgcgcat ctggcgctcg ccaaatacgg ggaactcgac cggcgcaatc
     1321 cgtttgtctc cttcggcgcc gcccggccgg gatgacctgg agcccagcgc cccggagagc
     1381 gatgcctcgg cgtgcgtgta aaggacgtta tcgtccatgc cctgtccccg cgacgatgac
     1441 ggcttcattc tacaggagcc gcgtccggca gcccgcgcgc cctgccgcta cttccccagc
     1501 gagcccggcg ggatcgaccg ttccggcgcg aagcgttgcc ttgcctgcat cgcctcgcgc
     1561 acgtccgagc gtaactgctc ttccttcatg cccttcggca ggaagcggaa aatctcgccc
     1621 ttgttgatcg cgtcggtcac gatccggacg tcggcgtcgc cgctcgtcat catgcgcatc
     1681 gtcctcggat gcagccgctt cacgcgctgc aggaattcca cgccctgcac gccgggcata
     1741 ttctggtccg agatcaccag gtccacgcga tgtcttccca gcaagcccag cgcctcgtgc
     1801 gcattgcggg cggtgagcaa ctggtatccg tccttggcga gcgaacgctt gagcagggcg
     1861 agcgtgtcgt cgtcgtcgtc caccagcaga acggttggcg cgaactccgc gggctgggac
     1921 agccgcacca ggcggcgctt ctcgccgagc cagctcccgc aatcatccgc cgtcagcggg
     1981 cgcgagaaat aatatccctg gatctcgtcg cagccgtttt ccgccaggaa ttcgaactgc
     2041 gactcggtct ccaccccttc ggccaccact ttcaggccga gactgtgcgc catcgagatt
     2101 accgcccggg tgatggtggc gtcgtcggga tcgctggtga tatcgcgcac gaaagaacgg
     2161 tcgatcttca gcgcgtccag cggaaagcgt ttcaggtagg ccaggctcga atacccggtg
     2221 ccgaaatcgt cgatcgagac gttgatcccg caggacttga ggtactggag cgtgctgatg
     2281 gctccatccg cattctgcat cagcgagctc tcggtgattt cgagttcgag gaagcgcggc
     2341 tcgatgcccg actcctcgat cgccgcgacg atgctcgccc cgagcttggg ggactggaac
     2401 tggcgcgccg acaggttgac cgccaccggc accggctcga cccccgaccg ctgccaggcc
     2461 ttgacctgcg cgcaggcggc cttgatgacc caatcgccca ccgggacgat cagcccggtt
     2521 tcctccagca ccggtatgaa ctcggctggc gatatcagcc cgcgttccgg gctctgccag
     2581 cgcagcaagg cctccacgcc ggtgatttcc cccgtggccg cgctggcctt gggctggtaa
     2641 tgaaggatga actcgccgcg gtcgagcgcc ctgcgcaggc tgctctccat gctgagcagc
     2701 gcctgcgccc gcgtattcat ctccggcatg tagaactgga agctgttgcg gccgagctcc
     2761 ttcgcccggt acatcgccgc atcggcgttc ttgatgagcg tgtcctgctc aacgctgtcg
     2821 tccggataga gcgtgatgcc gatactcgcc gtcacgtaga tttcggcgct gtccagcttg
     2881 aacggctcgt tgaagcccgc catgattttc tgggccacca ggttcgcgtc ctgggcggag
     2941 gtcaggttcg acagcacgat cgagaactcg tcgcctccca ggcggcccac ggtgtcgccc
     3001 gcgcgcaccg ctccggtcag gcgctcggag acctgttgca gcagcttgtc gcccatcgcg
     3061 tggcccagcg tatcgttcac gttcttgaac cggtcgacgt cgatgaacag cacgccgacg
     3121 ttccactggt tgcgcctggc ctgcgcgagc gcctgcttca ggcggtcgta aaacagcacg
     3181 cgattgggca ggttcgtcaa cacgtcgtag tgggcgagct gcatcagctg cgtttccgcc
     3241 agcttgcgct cggtgacgtc ttccgcaatg cccgcaatac ggtacaccgc gccggtgtcg
     3301 tcctgaaccg gaaacgcgcg gtcgtgcacc cagcgcaccg agccgtccgg ccgcacgatc
     3361 cggaaactct cgtcgtaacc gccaccggtc gccgacttcc tggcattctt cacccgcgcg
     3421 cggtcctcct cgtgcacgct gtcgagccag ctgcgcgcgt tcctgtggat ctggtcgagc
     3481 ggccgcccgg tgatctcttc ccatgccggg ctcacgtaaa tcgcctcgtg ctgctcgacg
     3541 tcggtaatcc agaacacctg cgggatgttt tccgccaatt gccggaattg ctgctccgac
     3601 tccttcagcg cagactccgc ctgcttgcgc tggacgaact ggccgatctg gcgcccgatc
     3661 gagccgctga tctggagcag catgctgtcc ggctggcgcg gctggcgtcc gaaaaactcc
     3721 atgacgccga tgacctcggt gccggcgatg atcgggaacg agtacgcgct gtgcaagccg
     3781 aatttgagcg ccgtctcctt gcgattgaac gattcctcgc gctggacatc ctcgatccat
     3841 gtcggctctt tttcgaacca ggcgcggcgc aacaggccgc ccggcgcctg gaatctcagc
     3901 ttgagccagg gtgtttcgtc cacgccagtg aaatccggga cgaaatcgct ccagtattcg
     3961 ccgcgcacga gggtgttctc ctccttgttc cataaccagc gcgcgccgta cgcccagccc
     4021 atgccatcgc acatggtcct gatcaggtgc ggcatcgcgg cttccagcgt cggcgcctcg
     4081 gcaagaacca ccgtcactgc gtgctccact tcgcggcgct gcgcggaaat cttgcgctcg
     4141 gtaatgtccc ggttcactcc aacgatgccg atgacgttgc ctgtggcgtc gcgcagggga
     4201 accttggacg cagcgaccca gcgtggattg ccggcgccgt ccaccgacga gcgctcgcgc
     4261 tcgatgaccg gcgtctcgga aaggaggatc gcgcggtctt cggcttcgat gcgctcggct
     4321 gccgcgcgcg gcatgatatc gaatatcgtc ttgcccagaa gctcctccgg gctcctggcc
     4381 ccatgcgccc tggcattggc catgttctgg agcagaaaac gcccctcccg gtccttgacg
     4441 tagatgcggt cgggcaccgc gtccacgagg gtgcgcagga gcgcacgctc gctcgcgatc
     4501 gcctcatccg cgtgcttgcg ctcggtgatg tcctcgacga tgcggatgaa ataatcgggc
     4561 ttgcccgatg cgtccctcac cagcgaaacg gtc
//
LOCUS       DBNGSG010000079         2059 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2404227, whole genome shotgun sequence.
ACCESSION   DBNGSG010000079 DBNGSG010000000
VERSION     DBNGSG010000079.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2404227"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="AC158Q_01985"
     CDS             complement(<1..316)
                     /locus_tag="AC158Q_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3338567.1"
                     /translation="MKPRITLITLGVDDLEKSLAFYRDGLGLETQGIVGREFEHGAVA
                     FFDLQSGLKLALWPRDSLAHDTKLGKAPRSPTDFSIGHNVGSKAEVDAVMAQAISAGA
                     SIV"
     gene            complement(447..851)
                     /locus_tag="AC158Q_01990"
     CDS             complement(447..851)
                     /locus_tag="AC158Q_01990"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3338568.1"
                     /translation="MARIRHIAIGTRDPEATARFYKEGLGLKEVGRLDSRTAEGYYLT
                     DGHVNVAILKFKSDDPATTEGGTRYTGVHHFGVEVEDMAQARARIEKAGAVHRPYPGT
                     EDMAKRGNIEVKVSGPDGVTIDLSEHGWLGTK"
     gene            complement(969..1937)
                     /locus_tag="AC158Q_01995"
     CDS             complement(969..1937)
                     /locus_tag="AC158Q_01995"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3338569.1"
                     /translation="MVRIAVSLILLAAAALPAGGALAQAYPTRPVRIIVPAAPGDSCD
                     VLSRLIGQKVGERLGHALTADNRVGAGGQVGLLLIAKAAPDGYTIGCGQGGNMVIVPI
                     ANKKVAYDSLRDFVPIQIPVTNFLGLVVHPTVPFKGVKDLVTYGKANPGKLVFGSNGE
                     GAFIHFSVELLRSLGGFTYLHVPYNGVAPIMTDLMSGRIDSTITSFVSVQPHVNAGRL
                     KLLAIGRATRSPKYPDYPTIAETLPGYENSGWFGFIAPAGTPREIVVLLNKEMNRAVM
                     LPDVRAKLDSYGLEIHTESPEFFANRIRSDIEKWGKVARDIGFQPK"
BASE COUNT          327 a          638 c          710 g          384 t
ORIGIN      
        1 tgacgatcga agcgccggcg cttattgcct gtgccatgac ggcatccact tccgccttgc
       61 tgccgacgtt gtgtccgatg gagaagtcgg tcgggctgcg gggcgccttc ccgagcttcg
      121 tgtcgtgcgc gaggctgtcg cgcggccaga gggcgagctt caggccggat tgcaggtcga
      181 agaaggccac ggcgccgtgc tcgaactccc tgccgacgat gccctgcgtc tccaggccca
      241 gcccgtcgcg gtagaacgcg agcgactttt ccaggtcgtc cacgccgaga gtaatcaagg
      301 tgatccgcgg cttcatttcg agtgacgagt gacgaaaacc ggggcgacta acggttaacg
      361 agaaacgagt aacaagaaac gagccacgcc ctttttagcc agggcgctct tactttcgac
      421 tttcgccttc cgactttcgc cttgtgctac ttcgttccca gccacccgtg ctcggagagg
      481 tcgatggtga cgccgtcggg cccggagacc ttgacctcga tgttgccgcg cttcgccatg
      541 tcttccgtcc cggggtaggg acggtgcacg gcgccggcct tctcgatgcg cgcccgcgcc
      601 tgcgccatgt cctcgacttc gacgccgaag tggtggacgc cggtgtaacg ggtgcccccc
      661 tccgtcgtgg cggggtcgtc ggacttgaac ttgaggatgg cgacgttcac gtggccgtcg
      721 gtcaggtaat agccctcggc ggtcctggag tcgaggcgcc cgacctcctt cagcccgagg
      781 ccctccttgt agaaccgcgc cgtggcttcc gggtcgcggg tgccgatcgc aatgtggcgg
      841 atgcgtgcca ttcgagtctc cttgaccatg ccgggctggg gcgacaccgc ctggggcggt
      901 gtcgccctct tccgattttc gaattaattc ctttgtgtcc tttgtgtcct ttgtgtcgag
      961 gcgttgcatc acttcggctg gaagccgatg tcccgcgcga ccttgcccca tttctcgatg
     1021 tcgctgcgga tccggttggc gaagaactcc ggcgattcgg tgtggatctc aaggccgtag
     1081 gagtccagct tcgcgcgcac gtctggcagc atgactgcgc ggttcatttc cttgttgagc
     1141 agcaccacga tctcgcgcgg ggtgccggcg ggcgcgatga agccgaacca gccgctattc
     1201 tcgtagccgg gcagcgtctc cgcgatcgtc gggtaatcgg ggtacttggg cgagcgcgtg
     1261 gcgcggccga tggcgaggag cttcagccgg ccggcgttga cgtggggctg gaccgagacg
     1321 aaggaggtga tcgtcgagtc gatccgcccg ctcatgaggt cggtcatgat gggcgccacg
     1381 ccgttgtagg gcacgtggag ataggtgaag ccccccaggc tgcgcagcag ctcgaccgag
     1441 aagtggatga aggcgccctc gccgttgctg ccgaaaacga gcttcccggg gttggccttg
     1501 ccgtaggtga cgaggtcctt gacgcccttg aagggcacgg tcgggtgcac caccaggccc
     1561 aggaaattgg tcaccgggat ctggatcggc acgaaatccc tgagcgagtc gtaggcgacc
     1621 ttcttgttgg cgatcggcac gatcaccatg ttgccgccct ggccgcagcc gatggtgtag
     1681 ccgtccggcg cggccttggc gatgagcagc aggccgacct ggccgccggc cccgacccgg
     1741 ttgtccgccg tgagcgcatg tcccaggcgc tcgccgacct tctgcccgat cagccgggac
     1801 agcacgtcgc agctgtcccc cggcgccgcg gggacgatga tgcgaaccgg ccgggttgga
     1861 taggcctgcg cgagggcgcc gcccgccggc agcgccgcgg cggccaacag gatcaacgag
     1921 acagcgatgc ggaccatggc cccctccctg gttttgacag tgcgggaaag tatggaccgg
     1981 cagaattaaa ggcacaaggc gcacgggata aggggaagga gggtcgaggc aggagtgtcg
     2041 agggaggaaa cgagggacg
//
LOCUS       DBNGSG010000080        11992 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_536908, whole genome shotgun sequence.
ACCESSION   DBNGSG010000080 DBNGSG010000000
VERSION     DBNGSG010000080.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 11992)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11992)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11992
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_536908"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="AC158Q_02000"
     CDS             complement(<1..258)
                     /locus_tag="AC158Q_02000"
                     /inference="COORDINATES: protein motif:HMM:NF021127.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HIR3338570.1"
                     /translation="MSITLFLTGDVNLMNVTGPSSPFARVTSTLKQADVRFANLECCF
                     YDPPGERSPGDEGFYAPPAAGEALKLAGFDAEGNANNENYGP"
     gene            435..875
                     /locus_tag="AC158Q_02005"
     CDS             435..875
                     /locus_tag="AC158Q_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3338571.1"
                     /translation="MFKNILIPTDGSELSQRAVRTAVELAKLHRARITGIHVIPDYHL
                     LIAYEGAFDPVTEERIEEEAKTRAEGYLAFVRKCAEDASVACDTACETSDHPYDAILK
                     TADSRGCDLIVMTSHGRKGLAAVLLGSETRKVLTHAKIPVLIVR"
     gene            1285..2454
                     /gene="sucC"
                     /locus_tag="AC158Q_02010"
     CDS             1285..2454
                     /gene="sucC"
                     /locus_tag="AC158Q_02010"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HIR3338572.1"
                     /translation="MKIHEYQAKEILRKYGVATPRAIPCFSVDEAVAAAEKLGGSVWV
                     VKAQIHAGGRGKGGGVKVAKSLDEVKGHAGRILGMQLKTHQTGPGGQKVRRLLIEEGA
                     DIKKELYLGMVVDRGSQRVALMASSEGGMDIEEVAARAPEKIHKIIIKDPFKGLADRD
                     AEDIARKIGVPHDSIPQARDLLKALYKAFDDTDASLAEINPLILTGDGRVVALDAKMN
                     FDYNALFRHPEIMALRDLDEEDPAEVEASKFDLNYIQLEGNIGCLVNGAGLAMATMDI
                     IKLYGGAPANFLDVGGGASTEKVTEAFKIMLKNPKLAAILVNIFGGIMKCDVIAQGVV
                     AAAREVKLSVPLVVRLEGTNVDLGKKILAESGLPIISGNNMADAAQKVVAATKGK"
     gene            2542..3420
                     /gene="sucD"
                     /locus_tag="AC158Q_02015"
     CDS             2542..3420
                     /gene="sucD"
                     /locus_tag="AC158Q_02015"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HIR3338573.1"
                     /translation="MSILINKNTRVMTQGITGKTGQFHTKMGKEYANGRNCYVAGVTP
                     KKGGQSYEGIPIFETVKEAKDKTGANVSVIYVPPPYAAAAIDEAVEAGLDLVICITEG
                     IPVRDMIRTHQKMLGKKTVLVGPNCPGVITPDEIKIGIMPGHIHKKGPIGVVSRSGTL
                     TYEAVGQLMEVGLGQSSCVGIGGDPVNGLKHIDVLKLFNDDPQTEAVIMVGEIGGANE
                     EEAARWVKANMKKPVVGFIAGVTAPPGKRMGHAGAIISGGKGTAEEKLAVMEACGIRT
                     TKNPAEMGKLLKSVLK"
     gene            3461..5143
                     /locus_tag="AC158Q_02020"
     CDS             3461..5143
                     /locus_tag="AC158Q_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HIR3338574.1"
                     /translation="MEGGSVKRKHDGNARGKLKGRAVDSRALDEVQALLKDGPRRRDL
                     LIEYLHKIQDRYGHLSAAHVAALAREMKLAQTEVYEVATFYHHFDVIKEGETPPPPLT
                     VRVCESLSCELSGAGELMSGLKRALGAGVRVIPAPCVGRCEQAPVAVVGQNPVARATV
                     EKVKPLADAGKTKCPVSRYVTYSDYRKKGGYRLAAECLAGKRDAESILKTMEDSGLRG
                     LGGAGFPAGRKWRLVRNETAPRLLAINIDEGEPGTFKDRYYIERDPHRFLEGAIIAAW
                     TAQISEAYIYLRDEYAGCREILERELKALQADPPCALPPIYLRRGAGAYICGEESAMI
                     ESIEGKRGMPRLRPPYVAQVGLFGYPTLEHNMETLHWVREILEKGAAWFASHGRNGRK
                     GLRSFSVSGRVRKPGVHLAPAGITVNELIEEYCGGMLPGHTFYAYLPGGASGGILPGS
                     MGDIPLDFDTLQPHGCFIGSAAIIILSDRDKAAAAARNLMKFFADESCGQCTPCRVGT
                     AKALKLMEAPRWDRPLLEELSVAMADASICGLGQAAPNPIRCVVKFFPQEIG"
     gene            5164..7980
                     /gene="fdhF"
                     /locus_tag="AC158Q_02025"
     CDS             5164..7980
                     /gene="fdhF"
                     /locus_tag="AC158Q_02025"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HIR3338575.1"
                     /translation="MTAMTKEELNAMPVEFRLNGKDVVAHGGETIIQTAKRLGVEIPH
                     LCYKEGMRPDGNCRACVVEVKGERVLAASCCRKPVKGMEVITDNERARHSQKMVLELL
                     LADMPKERHTLASELDQWVAKLKVGKPRFAPRHQPKADLSHYGIAVNLDSCIQCTRCL
                     RACREEQVNDVIGYAFRGEHAKIVFDLDDPMGDSTCVACGECVQACPTGALMPARKVG
                     LVKPDKQVHSVCPYCGVGCQLTYAVKDNRILYVDGRDGPSNHGRLCVKGRYGFDYVHH
                     QQRLLRPLIRKPGTPKHADFTMDPSKPLEHFREASWEEALDLAAGKLTSIRDQHGKGA
                     LAGFGSAKGTNEEAYLFQKLVRTGFGSNNVDHCTRLCHASSVAALMEGVASGAVSNQV
                     NDVARAEVIFLIGANPVSNHPVAATWIKNAAKNGAKLIYADPRRSELSRHAHIMLQFK
                     PDTDVALLNAMMHTIVHEGLVNEDFIKSRTIGYEDLKKNVAGYSPEAMAPVCGIPAGT
                     IREVARLFANSKASMILWGMGISQHIHGTDNARCLIALCMVTGQIGRPGTGLHPLRGQ
                     NNVQGASDSGLIPMVFPDYQRVDNAEARARFEKLWGAALDPKPGLTVVEIVHAVLEGK
                     IRGMYVMGENPAMSDPDAHHAREALAKLDHLVVQEIFLTETCYYADVILPATAWPEKD
                     GTVTNTDRMVQLGRRALDAPGEAKEDLWIIQELARRMGLDWNYSGPRDVFNEMRKGMN
                     SIAGITWERLEEESCVTYPCEKEGDPGQPVVFIDKFPTPTGRARLVPVDIIPAAERPD
                     RDFPFVLITGRQLEHWHTGAITRRATVLDAIEPEPTASIHPLDLDAMGAKPGDVVTVE
                     SRRGIISLYARADDGTPRGAVFIPFCFYEAAANLLTNPALDPFGKIPEFKYCAVKVMK
                     GGELTPRSSYGGGQALAQLEKIQR"
     gene            8104..9780
                     /locus_tag="AC158Q_02030"
     CDS             8104..9780
                     /locus_tag="AC158Q_02030"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HIR3338576.1"
                     /translation="MPLSDIEIAQQAKMLRATKIAEKLGIPEEHLVPYGHYKTKVSLD
                     FVDSLARKKNGKLILVTAITPTPAGEGKTTTTVGLGDALNHIGKKAIICLREPSLGPV
                     FGMKGGAAGGGYARVVPMEDINLHFTGDFNAIGLANNLLAAALDNHIHHGNELGIDVR
                     RITWKRVMDMNDRALRDITVSLGGPGNGYPRQDGFDIVVASEVMAIFCLATSLKDLKE
                     RLGNIVIGYTRDQKPVRAMDLKVHGAMTVLLKDALAPNLVQTLENCPAFIHGGPFANI
                     AHGCNSVIATQTALKLADYVVTEAGFGADLGAEKFIDIKCRKSGLRPDAVVIVATVRA
                     LKYHGGVDLKEVNKENLAALEKGITNLERHVNNVRNHYGLPCVVSINRFNFDTPAEID
                     LLKKKMAHHEAPVILATHWADGGKGAAEVARAVVDLIEKVPSDFKFVYEDKLPLWDKI
                     GTIATKIYGASEVTADAKVRGQIKKLQEDGYGHYPVCVAKTQYSFSTDPKLRAAPSGH
                     VINVREVRLAAGAEFVVMVCGDVMTMPGLPKVPSAEKIDLTDDGKVVGLF"
     gene            10112..11494
                     /locus_tag="AC158Q_02035"
     CDS             10112..11494
                     /locus_tag="AC158Q_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HIR3338577.1"
                     /translation="MQYREIERGVQRRALPMAALAAFLLLAALPAAAATGEGPAVLGI
                     PVDFILFALTLLGVALFHHHTLRVALTGLTVIALYKIIFTGFKTGAGFGGFVMHLGHE
                     WVILTNLLGLLVGFALLSNHFEKSHVPQVLPRFLPDDWKGAFVLLVMVWVLSGFLDNI
                     AAALIGGTVAGTVFRHKVHIGYLAAIVAASNAGGAGSVVGDTTTTMMWLDGVSPLDVL
                     HAYVAAAAALLVFGIPASLQQQRYAPIVRHEGRHAPVDWARLAIVALMLVSAIATNVM
                     VNWKFKHLADAFPFIGAAVWIALLVCVPWRKPQWDLVPEAFKGSIFLLSLVLCASMMP
                     VEKLPVASWQSSLGLGFISSVFDNIPLTALALKQGGYDWGVLAYAVGFGGSMIWFGSS
                     AGVALSNMYPQARSVGLWLRHGWHIAIAYVVGFFVMLAILGWEPHEKHKSDAAPAPVT
                     VSSSAPASTC"
     gene            11510..>11992
                     /locus_tag="AC158Q_02040"
     CDS             11510..>11992
                     /locus_tag="AC158Q_02040"
                     /inference="COORDINATES: protein motif:HMM:NF013132.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HIR3338578.1"
                     /translation="MEPMQWLTLIVFGLTILAVITNVIDSTLAALIGVAVMCWLGIMT
                     ETDAFKYVDWNVMAILVGIWIIAGYFGKSGVPNWLSVQALKLSGGRPGLLVVILTLLA
                     GVISMFVDNVVTILMMAPVALPLARALKLPVVPVILMIGFGANFMGTALIIGDLPPQM
                     L"
BASE COUNT         2330 a         3663 c         3947 g         2052 t
ORIGIN      
        1 ggggccgtag ttttcgttgt tggcattgcc ctcggcgtca aaaccggcga gcttgagcgc
       61 ttcgccggcc gcaggcggcg cgtagaagcc ctcgtcaccg ggggaacgct cgcccggcgg
      121 gtcgtagaag cagcactcca ggttggcgaa ccgcacgtcc gcctgcttga gagttgacgt
      181 aacacgggca aagggactgc tcgggccggt aacgttcatc agattgacgt caccggtgag
      241 gaaaagagta atgctcaaga gatctccgac cgtatcgcgg aaaagcgatc atataccagg
      301 cctgcgatcc tccctccagc ccgcggttgg gtcgaccgac ccttgacagt caacgcgggg
      361 agcgacaagc ttcacttgag gtccaagtcg tggaaccagg gccaagctag caccctggcg
      421 cagaggagga agcgatgttc aagaacatcc tgataccgac cgatggttcg gaactctcgc
      481 aacgggcagt gcgcaccgcg gtggagctcg caaagctgca ccgggcgcga atcaccggca
      541 tacacgtcat tcccgactac cacctcctga tcgcatacga gggtgctttc gatccggtca
      601 ccgaggagcg tatcgaggag gaggcgaaga cccgcgcgga aggttatctg gcgttcgtgc
      661 gcaagtgcgc cgaggacgcg agtgtggcgt gcgataccgc atgcgaaacc agtgaccacc
      721 cctatgacgc gatattgaag accgcggatt cgcgcggatg cgacctcatc gtgatgacgt
      781 cgcatgggcg caaaggcctg gctgcggtgc tgctgggcag cgagacgcgc aaggtgctga
      841 cccatgccaa gattccggtg ttgatcgtaa gataaataca agtatatgat taataatgta
      901 taataatgat aatatgtgaa ttattgcaca gcagcagcaa acgcttgcat tagatcccgg
      961 aacgtggaat aatgcgctcg tgctggcgcc tctccggcgt tggcgcactc ctccaaaatc
     1021 ctcctccttt ggcggaacct taagcgcaac cttcaccggt tgcgcttttt tttgcttccg
     1081 actcgcatgg atttcctcct cgcccccaca tggcaccgcg aagcggtcgc ggcgtttcgg
     1141 tttgttatac tgccccgttc caaatcgcgg cgcgcactgc accacttccg ggcggagccg
     1201 gcgttgactt cggtcaaggc cgctgcgcta tggtctgtat agagtcccgg ctgaaaccgc
     1261 ggcaccgaac cacacgggtc acagatgaaa atccacgagt accaggccaa ggaaatcctg
     1321 cgcaagtacg gtgtcgccac gcctcgcgcc atcccgtgct tcagcgttga cgaggccgtg
     1381 gcagccgcgg aaaaactcgg cggaagcgtg tgggtggtga aggcccagat tcatgccggc
     1441 ggccgtggca agggcggcgg cgtcaaggtt gccaaatcgc tggacgaagt gaagggccac
     1501 gccggccgga tcctcggcat gcaactgaag acgcaccaga ccggtcccgg gggccagaag
     1561 gtgcggcggc ttttgatcga ggagggcgcg gacatcaaga aagagctcta cctcggcatg
     1621 gtggtggatc gcggctcgca gcgcgtggcg ctgatggctt ccagcgaagg cggcatggac
     1681 atcgaggaag ttgcggcaag ggcgccggag aagattcaca agatcatcat caaggacccc
     1741 ttcaagggtc tggccgaccg ggacgccgag gatatcgccc gcaagatcgg cgtgccgcat
     1801 gacagcattc cgcaggcgcg ggacctcctg aaggccctgt acaaggcgtt tgacgacacc
     1861 gacgcctcgc tcgcggagat caatccgctg atcctgaccg gcgatggcag ggtcgtggcg
     1921 cttgatgcaa agatgaattt cgactacaac gcgctgttcc gccaccccga gatcatggcg
     1981 ttgcgcgacc tcgacgagga ggacccggcc gaggtcgagg cctccaagtt cgacctcaat
     2041 tacatccagc ttgagggcaa catcggctgc ctggtgaacg gggcgggatt ggccatggcg
     2101 accatggaca tcatcaagct ctacggcggc gcgcccgcca attttctcga cgtaggcggc
     2161 ggggcatcca ccgaaaaggt gacggaagcg ttcaagatca tgttgaagaa tcccaagctg
     2221 gctgcgattc tggtcaacat cttcggcggc atcatgaagt gcgacgtgat cgcgcaaggg
     2281 gtcgtggcgg cggcgcgcga agtgaagtta agcgtaccgc tggtggtgcg gctggaaggc
     2341 accaatgtcg acctgggcaa gaagatactc gccgagtcgg gattgcccat catctccgga
     2401 aacaatatgg ccgatgccgc acaaaaggtg gtcgcggcca ccaagggcaa gtgacctcgt
     2461 gtggccgggc ccgtggatgc gttttcccta tgaaaacagg gggagagtga ccactcactt
     2521 ttggctggaa aaacaagcga tatgagcatc ctgatcaata agaacacccg ggtgatgaca
     2581 cagggcatca ccggcaagac cgggcaattc cataccaaga tgggtaagga atatgccaac
     2641 ggccggaact gctacgttgc cggcgttaca ccgaagaagg gcgggcagag ctacgagggc
     2701 atacccatct tcgaaacggt caaggaagcg aaggacaaga ccggcgccaa cgtttccgtg
     2761 atctacgtac cgcctcctta tgccgccgcg gcgatcgacg aggcggtgga agcggggctt
     2821 gacctcgtca tctgcatcac cgaaggcatc ccggtacgcg acatgatccg cacccaccag
     2881 aaaatgctgg ggaaaaagac ggtcctggtc gggcccaact gcccgggcgt gatcacgccg
     2941 gacgagatca agatcggcat catgcccggc catatccaca agaagggccc gatcggggtg
     3001 gtttcgcgct cgggcacgct gacctatgaa gctgtgggcc agctgatgga ggtgggcctc
     3061 gggcagtctt cctgcgtggg catcggcggc gacccggtga acggcctgaa gcacattgac
     3121 gtgttgaagc tattcaacga cgacccgcag accgaagccg tcatcatggt gggcgagatc
     3181 ggcggagcca atgaggaaga ggcggcgcgc tgggtcaaag ccaatatgaa gaagcccgtc
     3241 gtcggcttca tcgccggcgt gacggcgcct ccgggcaagc gcatgggcca cgcgggcgcc
     3301 attatttccg gcggcaaggg cacggccgag gaaaagctcg ccgtgatgga ggcgtgcggg
     3361 atcaggacaa ccaagaaccc ggctgaaatg ggcaagctgc tcaagtcggt tctgaaataa
     3421 tatccggcgg gccttgcgtg cccgcctgcg aggaggatgg ttggaaggcg gtagcgtcaa
     3481 aaggaaacac gatggcaatg cgcgcggcaa gctcaagggg cgtgcggttg actcccgggc
     3541 actggacgag gtccaggcac tgctgaaaga cgggccgcgg cgccgcgacc tgctgatcga
     3601 gtacctgcac aagatccagg accgctacgg gcatctttca gccgcccacg tggcggcgct
     3661 cgcgcgcgag atgaagctcg cgcagaccga ggtctacgag gtcgccacct tctatcatca
     3721 tttcgacgtc atcaaggaag gcgagacgcc gccgccgcca ctcaccgtgc gggtgtgcga
     3781 atcgctctcc tgcgagctgt caggcgccgg cgagctgatg tccggactca agcgcgcgct
     3841 gggggcgggg gtgcgcgtca ttccggcccc ctgcgtcggc cgctgcgagc aggccccggt
     3901 cgccgtggtg gggcagaacc cggtggcgcg ggcgacggtg gagaaggtga agccgctggc
     3961 cgacgcgggc aagacgaagt gcccggtgtc ccgctacgtc acatactccg attaccggaa
     4021 gaagggcggc taccggcttg ccgccgagtg cctcgccggc aagcgcgatg ccgaaagcat
     4081 cctgaagacg atggaggatt ccggcctgcg cgggctcggc ggcgcaggct ttcccgccgg
     4141 gcgcaagtgg cgcctggtga gaaacgagac ggcaccgcgg ctgctcgcga tcaacatcga
     4201 cgagggtgaa ccgggcactt tcaaggaccg ctattacatc gagcgcgacc cgcatcgctt
     4261 cctggaaggg gcgatcatcg cggcctggac ggcacagatt tccgaagcct atatctacct
     4321 gcgcgacgag tacgcgggct gccgcgagat cctcgagcgc gaattgaaag cgctgcaggc
     4381 cgacccgccg tgcgcgttgc cgccgattta ccttcggcgt ggcgccggcg cctacatctg
     4441 cggcgaggaa tccgcgatga tcgaatcgat cgagggcaag cgcggcatgc cgcggctgcg
     4501 ccccccgtac gtcgcgcagg tgggtctgtt cggctatccc acgctcgagc acaacatgga
     4561 gacgctccac tgggtgcgcg aaatcctgga gaagggcgcc gcgtggttcg cctcccacgg
     4621 ccgcaacggg cgcaagggac tgcgctcgtt ctcggtctcg ggccgggtaa ggaagcccgg
     4681 ggtgcacctg gcgcccgcgg gcatcacggt gaacgaattg atcgaggagt actgcggcgg
     4741 catgctgccg gggcacacct tctacgccta cctgccgggc ggcgcgtcgg gcgggatact
     4801 gccgggctcg atgggcgaca tccccctcga tttcgacacc ttgcagccgc acggctgctt
     4861 catcggctcg gcggccatta ttatcctgtc ggaccgggac aaggctgcgg ccgccgcgcg
     4921 gaacctgatg aagttcttcg ctgacgaatc ctgtgggcag tgcacgccct gccgggtggg
     4981 cacggccaag gcgctgaagc tcatggaagc gccgcgctgg gaccggccgc tgctggagga
     5041 gctttcggtg gcgatggcgg acgcttccat ctgcgggctg ggacaggcgg cgccgaaccc
     5101 gatacgctgt gtggtgaaat tttttccgca ggagatcggc tgaaaccggc cggagacgag
     5161 cacatgactg cgatgacgaa ggaagaattg aacgcgatgc cggtggagtt caggctgaac
     5221 ggcaaggacg tggtagccca cggcggcgag accatcatcc agaccgccaa gcgtctcggc
     5281 gtggagatcc cgcatctctg ctacaaggag ggcatgcgcc cggacggcaa ctgccgggcc
     5341 tgcgtggtcg aggtgaaggg cgagcgcgtg ctggcggctt cgtgctgccg caagccggtg
     5401 aagggcatgg aagtcatcac cgacaacgaa cgcgcgcggc actcgcaaaa gatggtgctg
     5461 gaactgctgc tcgccgacat gccgaaggag cgccatacgc tcgcttccga gctcgatcaa
     5521 tgggtcgcca agctcaaggt cgggaagcct cgcttcgcgc cgcggcacca gccgaaagcc
     5581 gatttgtctc actatggcat cgcggtcaac ctcgattcct gcatccagtg cacgcgctgc
     5641 ctgcgcgcct gccgcgagga gcaggtgaac gacgtcatcg gctacgcctt ccgcggcgag
     5701 cacgccaaga tcgtgttcga cctcgacgac ccgatgggcg attccacgtg cgtcgcctgc
     5761 ggcgagtgcg tgcaggcgtg cccgaccggg gcgctgatgc cggcacgcaa ggtcggcttg
     5821 gtgaaacccg acaagcaggt gcactcggtc tgcccgtact gcggcgtcgg ctgccagctg
     5881 acctatgcgg tcaaggacaa caggattctc tacgtcgacg ggcgcgatgg cccgtcaaac
     5941 cacgggcggc tgtgcgtcaa gggccgctat ggtttcgatt acgtgcatca ccagcagcgg
     6001 ctcttgaggc cgctgatccg caaacccggc acgcccaagc atgccgactt caccatggac
     6061 ccttcgaagc cgctcgagca ctttcgcgaa gcgagctggg aggaagcgct cgatctggcc
     6121 gcgggcaaac tgacgtccat acgcgatcag catggcaagg gcgcgctcgc cggcttcgga
     6181 tcagccaagg gcaccaacga ggaggcgtat ctcttccaga aactggtgcg cacggggttc
     6241 ggctcgaaca acgtggacca ctgcacgcgg ctgtgccacg cctccagcgt tgccgcgctg
     6301 atggaaggcg ttgcctcggg cgcggtgtcg aaccaggtga acgatgtcgc ccgcgcggaa
     6361 gtgatcttcc tcatcggcgc gaacccggta tcgaaccatc cggtcgcggc aacctggatc
     6421 aagaacgcgg ccaagaacgg cgccaagctc atctacgccg acccgcgccg ctcggagctg
     6481 tcacgccacg cgcatatcat gctgcagttc aagcccgaca cggacgtcgc gctgctgaat
     6541 gccatgatgc acaccatcgt gcacgagggc ctggtcaatg aggatttcat caagagccgc
     6601 accattggct acgaggacct gaagaagaac gtcgcgggtt acagccccga ggcgatggcg
     6661 ccggtctgcg gcataccggc agggaccatc agggaagtgg cgcgcctgtt cgccaactcg
     6721 aaggcctcga tgatcctgtg gggcatgggc atttcgcagc acatccacgg caccgataac
     6781 gcgcgctgcc tgatcgcgct gtgcatggtg accggccaga tcgggcgtcc cggcaccggg
     6841 ctgcacccgc tgcgcgggca gaacaacgtg cagggcgcat ccgattccgg cctgatcccg
     6901 atggtgttcc cggactacca gcgcgtcgac aatgccgaag cgcgcgcgcg tttcgagaag
     6961 ctctggggcg ccgccctcga tccgaaaccg gggctgacgg tggtcgagat cgtacacgcg
     7021 gtgctcgaag gcaagatccg cggcatgtac gtcatgggcg agaatccggc gatgtccgac
     7081 ccggacgcgc accacgcgcg cgaggcgctt gccaagctcg accacctggt cgtccaggaa
     7141 atcttcctca ccgagacctg ctattacgcc gatgtgatcc tcccggcgac cgcctggccg
     7201 gagaaggacg gcacggtcac caataccgac cgcatggtgc aacttgggcg cagggcgctc
     7261 gacgcgccgg gcgaagcgaa ggaagatctc tggatcatcc aggagctggc gcggcgcatg
     7321 ggcctcgact ggaattacag cggcccgcgc gatgtcttca acgagatgcg caagggcatg
     7381 aactcgatcg ccggcatcac ctgggagcgc ctggaagaag agagctgcgt gacctatccc
     7441 tgcgagaagg aaggcgaccc ggggcagccg gtggtgttca tcgacaagtt tccgacgccg
     7501 accgggcgcg caaggctggt gccggtagat atcattcccg cggcggaacg gccggaccgc
     7561 gattttccgt tcgtgctcat caccggccgc cagctcgagc actggcatac cggggcaatc
     7621 acgcgccggg caaccgtgct cgacgcgatc gagccggaac cgacagcctc gatacacccg
     7681 ctcgacctcg acgcgatggg cgccaagccc ggcgacgtgg tcacggtgga atcgcggcgc
     7741 ggcatcatct cgctctacgc gcgcgccgac gacggcacgc cgcgcggcgc ggtgttcatc
     7801 ccgttctgct tctacgaggc ggccgcgaac ctgctgacca acccggcgct cgacccgttt
     7861 ggcaagattc ccgagttcaa gtattgcgcg gtgaaggtga tgaagggcgg ggagctgacg
     7921 ccgcgctcta gctacggcgg cggtcaggct ctcgcgcagc tggaaaaaat ccagagatag
     7981 ccgccaagac gccaggacgc ggaggaaccg gcagcacgtt cgtcaggcag cgtcgacttg
     8041 agtgattgcc ccgccgtgtc tgggaccaaa ttttgtagtt gaaatcagga gaggaagaac
     8101 aacatgccat tgtccgacat cgaaatcgcg cagcaggcca agatgttgcg cgccaccaag
     8161 attgccgaga agctcggcat accggaagag catctcgtcc cttacggcca ctacaagacg
     8221 aaggtgtcgc tcgatttcgt ggacagtctt gccaggaaga aaaacggcaa gctcatactg
     8281 gtcacggcga ttacgccgac accggcgggc gagggcaaga cgactaccac cgtcggcctg
     8341 ggcgacgcac tcaaccacat cggcaagaag gcgatcattt gcctgcgcga acccagcctc
     8401 ggcccggtgt tcggaatgaa gggcggtgca gcgggcggag gctacgcgcg ggtcgtgccg
     8461 atggaggata tcaatctgca cttcacgggc gatttcaacg cgatcggact cgccaacaac
     8521 ctgctcgccg ccgcgctcga caaccacatc caccacggca atgagctcgg gattgacgtt
     8581 cggcgcatca cgtggaaacg ggtgatggac atgaacgacc gggcgctgcg cgatatcacg
     8641 gtatcgctcg gcggccccgg caatggttat ccgcggcagg acggcttcga catcgtcgtg
     8701 gcatccgaag tgatggcgat tttctgcctg gcgacctcgc tgaaagacct caaggagcgg
     8761 ctcggcaaca tcgtcatcgg ctacacccgc gaccagaaac cggtgcgcgc catggacctc
     8821 aaggttcacg gcgcgatgac ggtattgctc aaggatgcgc tcgcgcccaa cctggtacag
     8881 acgctggaga actgcccggc tttcatccat ggcggaccgt tcgccaacat cgctcacggc
     8941 tgcaactcgg tgatcgcaac ccagacggcg ctgaagctcg ccgattacgt ggtgacggaa
     9001 gccggcttcg gcgccgacct tggcgcggag aagttcatcg acatcaagtg ccgcaagtcg
     9061 ggactgcggc cggatgccgt ggtgatcgtg gcaacggtac gcgcgctcaa ataccacggt
     9121 ggcgtcgacc tcaaggaggt caacaaggaa aacctcgcgg cattggagaa aggcatcacc
     9181 aacctggaac gccacgtcaa caacgtgcgc aaccactatg ggctgccgtg cgtggtgtcg
     9241 atcaatcgct tcaacttcga cacccccgcg gaaatcgatc tgctcaagaa gaaaatggcc
     9301 catcacgagg cgccggtgat tctggcgact cactgggcgg acggcggcaa gggcgcggcc
     9361 gaagtcgcac gcgccgtggt ggacctgatc gaaaaagtgc cgtcggactt caagttcgtc
     9421 tacgaagaca agctgccgtt gtgggataag atcgggacca tcgctaccaa gatctacggc
     9481 gcgagcgagg tcaccgcgga cgccaaggtg cgcggccaga tcaagaagct gcaggaagac
     9541 ggctacggtc actatcccgt gtgcgtggcg aagacccagt actcgttttc gacggacccg
     9601 aaattgcgcg ccgcgccttc gggccacgtg atcaacgtcc gcgaagtgcg gcttgctgcc
     9661 ggggccgaat tcgtcgtcat ggtgtgtggc gacgtcatga ccatgccggg tctgcccaag
     9721 gtgccatcgg ccgaaaaaat cgatctcacg gatgacggca aggtggtcgg gctgttctga
     9781 ttgcccgtca gtctcggcgg tattggttga cgcgccggaa acggccgtgg taaaaacgct
     9841 cgcgcgttgc gggtttcgac ctgcagcgcc cctccaaagg ggagtagcta cggcggtgcg
     9901 caggagcgcc gctgcggcag ggtcgtcatc acgggatacg gggggaaagc ctctgtgtcc
     9961 tcggctccgc cggcaacccg gaagttgcaa gcgagacctt tgcctggacg agcgccgcca
    10021 tgcctcgtcc gaggtgaagg ctgccctgcg acaagtgcga gaatcgctcc cagcacgcgg
    10081 ccatgtataa cccgtaactg aaggggaacg catgcaatac cgggaaatcg aacgcggtgt
    10141 gcaacgacgg gcgctgccca tggccgcgct ggcggcgttt ctcctgcttg cggctttacc
    10201 ggcagccgct gcgacgggcg aggggccggc cgtgctcggc atacccgtcg atttcattct
    10261 gttcgcgctg accctgctcg gcgtcgccct gttccatcac cacaccctgc gggtggcgct
    10321 gaccgggctc acggtaattg cgctctacaa gatcattttc accgggttca agaccggagc
    10381 gggcttcggc ggcttcgtca tgcacctcgg ccacgaatgg gtgatcctca ccaacctgct
    10441 gggcctgctg gtgggattcg ccctgctgtc gaaccatttc gagaagagcc atgtgccgca
    10501 ggtgctgccg cgtttcctgc ccgacgactg gaagggggcg ttcgtgctgc tggtcatggt
    10561 atgggtgctc tcggggtttc tggacaacat cgccgctgcg ctgattggcg gcaccgtggc
    10621 cggcacggtg ttccggcaca aggtgcacat cggttatctg gccgcgattg tcgccgcgtc
    10681 gaacgcgggc ggcgcgggca gcgttgtagg cgacaccacg accaccatga tgtggctgga
    10741 cggggtgagc ccgctcgacg tgctgcatgc ctacgtggct gcggccgccg ccttgctggt
    10801 gttcgggatt ccggcgtcgc tgcagcagca gcgttatgcg cccatcgtga ggcacgaagg
    10861 gcgtcacgcg ccggtggact gggcgcggct ggccatcgtc gcgctcatgc tcgtttcggc
    10921 gatcgcgacc aacgtgatgg tcaactggaa gttcaagcac ctggcggacg cgtttccgtt
    10981 catcggcgcg gccgtctgga tcgcgctgct cgtctgcgtg ccctggcgca agccccaatg
    11041 ggatctcgtg ccggaagcct tcaagggcag catcttcctg ctctcgctgg tgctgtgcgc
    11101 ctcgatgatg ccggtggaaa agctgcccgt cgcctcctgg cagtcgtcgc tggggctcgg
    11161 cttcatttct tcggtgttcg acaatattcc gctcacggcg ctggcgctga agcagggcgg
    11221 ctatgactgg ggcgttctcg cgtacgcggt gggcttcggc ggatcgatga tctggttcgg
    11281 ctccagcgca ggcgtcgcgc tgtccaatat gtatccgcag gcgcgttcgg tgggattgtg
    11341 gctacgccac gggtggcaca ttgcgatcgc ctacgtggtt ggctttttcg tcatgctggc
    11401 gatcctgggc tgggagccgc acgagaaaca caagagcgac gcggctcctg ctcctgtcac
    11461 ggtgagttcg agtgccccag catcaacctg ctaaaaaaga gactaatcca tggaaccgat
    11521 gcaatggtta acgctgatcg tattcggtct gacgatcctg gccgtaatca ccaatgtcat
    11581 cgacagcacg ctcgccgcgc tgatcggcgt ggcggtcatg tgctggctcg gcatcatgac
    11641 cgagaccgat gctttcaagt acgtggactg gaacgtgatg gcgatcctgg tcggcatctg
    11701 gatcatcgcc ggttacttcg gcaagtccgg cgtgccgaac tggctttccg tccaggcgct
    11761 gaagctgtcc ggcggccggc cgggtctgct ggtcgtcatc ctgacgctgc tggccggcgt
    11821 catctcgatg ttcgtggata acgtggtgac gatcctgatg atggccccgg tcgcgcttcc
    11881 cctcgcgcgc gcattgaagc tgccggtcgt gcccgtcatc ctgatgatcg gctttggcgc
    11941 gaacttcatg ggcaccgcgc tcatcatcgg cgacctgccg ccgcagatgc tg
//
LOCUS       DBNGSG010000081         4605 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_707724, whole genome shotgun sequence.
ACCESSION   DBNGSG010000081 DBNGSG010000000
VERSION     DBNGSG010000081.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4605)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4605
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_707724"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(236..1945)
                     /locus_tag="AC158Q_02045"
     CDS             complement(236..1945)
                     /locus_tag="AC158Q_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HIR3338579.1"
                     /translation="MAEQRKKLRLGELLVQQGLITTDQLSIALAEQRHNNVPIGRLLV
                     RLGFVTESAIRDIMARTIGQESIDLAQVVADPEALKLVNQDFARRNRVLPIAYDSAEQ
                     VLTIATTEIFNVVAMDQLRGMLGAQVEIKTLLAAEAQLGDYIDNFYGYELSVDGILKE
                     IETGEIDYESLQVGGGGDEYTQPMVRLVNALLVDAVKRGASDIHFEPEYAFMRIRYRI
                     DGVLETVRSLHKTYMPGITVRLKVVSNLNIAETRSPQDGRLSLTLNGRPIDFRVSTQP
                     TIYGENIVLRVLDREKSIISLDRMQLSRDSLEKINLMLARPEGILIVTGPTGSGKTTT
                     LYSLLAQVNDETVNIMTLEDPVEYPLTLMRQTSVSEVARMDFADGIRAIMRQDPDIIL
                     VGEVRDKETAEMAFRAAMTGHQVFTTLHTNSALGAFPRLLDIGIQPDIMAGNIIGIIA
                     QRLVRVLCSHCKEAYAPSREERQLLGIAGDQVSYLHRSVGCKQCDNKGFKGRTTVMEI
                     LVMDGDLDELVARRATAKELRLAAFGKGYRSLAEEGLQRVVEGSTSLVEVAREIDLTG
                     RHG"
     gene            complement(2195..3169)
                     /locus_tag="AC158Q_02050"
     CDS             complement(2195..3169)
                     /locus_tag="AC158Q_02050"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3338580.1"
                     /translation="MQAQTASRPATGGASAYVREHPVMVFGTLAGLAAIGFGIYVYLQ
                     IFHPQLFIGRPPITAPMGPLPPLVQAPAPAAPATTVAPPLSSAPLLKEAAMEPAPAAA
                     ARPKPQPAAPVAAREAGPRNAISVSRSTPEPVLNPLHTQAYAALQSGRSEEAQRLYGQ
                     LLDAEPKNVDALLGLAAIASRQGNVEEASRRYLQILELEPRHALAQSGLIALLGGADP
                     LAAESKLRQLIAREPSAHLYFTLGNLYAGQSQWAAAQQAYFQAHHLEPANPDYAYNLA
                     VALEHVSQPKLALGFYRRAAQLAAARGRVNFNVSQAQERIGKLAAQVE"
     gene            complement(3442..>4605)
                     /locus_tag="AC158Q_02055"
     CDS             complement(3442..>4605)
                     /locus_tag="AC158Q_02055"
                     /inference="COORDINATES: protein motif:HMM:NF012485.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338581.1"
                     /translation="ERLALVKQEQELRTKQIEAASRAGQGAPNLAASVISGAPGSGAQ
                     QQASLLPDDVVVNSVSGTITINATERQHQLVQQHIDSIVSAMQRQVLIEATIVEVQLS
                     NNYQGGIDWSRLALNGGFSFIQSLLTGFGTGLNQGGANFFQIGYSNPNTRGGNISATV
                     KLLEEFGNTRVLSSPKLMALNNQTALLKVVDNIVYFEIRADTTTTQGAASTTFNTTAK
                     TVAVGVVMGVTPQINEDGRVSITVRPTISRVLRFVNDPNPSLANPCGIPVPTLGCNIT
                     PINSPVPEIQVREMESVLQVGSGQTVILGGLMQDSVRRDREQIPGADQIGIAGELFRY
                     RDESVTKSELVIFLRPTVVANPSLESDELRFFQRFLPQPEASPPQPEKAGAAR"
BASE COUNT          767 a         1409 c         1537 g          892 t
ORIGIN      
        1 ctccgccgcg cgcgtgccgg ccggccttgt ctatcgcttt gtattcgtaa gaaggcaatg
       61 gaaaacagtg ttgagtgttt agttttgagt gttgagttat agaggcaggt tcggtcgggg
      121 ccaactaaag aaaacagtgt tgagttttga gtgtttagtg ttgagttatc aggcagtgcc
      181 gaggaaggtc gatctaaaac tcaaacttaa cacttaacac tcaacactcg gggcgtcagc
      241 catgacgccc cgtaaggtcg atctcgcgcg cgacttcgac caggctggtg ctgccttcga
      301 ccacccgctg cagcccctcc tcggcgagcg agcggtagcc cttgccgaac gccgccaagc
      361 gcagttcctt cgcggtggcg cggcgcgcca ccagctcgtc gagatcgccg tccatcacca
      421 ggatctccat gacggtggtg cggcccttga aacccttgtt gtcgcactgc ttgcagccga
      481 ccgagcggtg caggtaggaa acctggtctc ccgcgatccc gagcagctgg cgctcctccc
      541 gggacggcgc ataagcttcc ttgcagtggc tgcacagcac ccgcaccagg cgctgcgcga
      601 tgatcccgat gatgttgccc gccatgatgt cgggctggat accgatgtcc agcagccgcg
      661 ggaaggcgcc gagcgcggag ttggtgtgca gcgtggtgaa cacctggtgg ccggtcatcg
      721 ccgcgcggaa cgccatttcc gcggtttcct tgtcgcgcac ctcgcccacc aggatgatgt
      781 ccgggtcctg gcgcatgatg gcgcggatgc cgtcggcgaa gtccatgcgc gcgacctcgc
      841 tcaccgaggt ctggcgcatc agtgtcagcg gatactcgac cgggtcctcc agggtcatga
      901 tgttgacggt ttcgtcgttg acctgcgcga gcagcgaata aagcgtcgtg gtcttgccgc
      961 tgccggtggg gccggtgacg atcagtatgc cctccggccg cgccagcatg aggttgatct
     1021 tctccagcga gtcccgggag agctgcatgc ggtcgagcga gatgatggat ttttcccggt
     1081 cgagcacgcg cagcacgatg ttctcgccgt agatggtggg ctgcgtcgaa acccggaaat
     1141 cgatcggccg cccgttgagc gtcaacgaga gccggccgtc ctgcggcgag cgcgtttccg
     1201 cgatgttcag gttgctcacc accttcagcc gcaccgtgat gccgggcatg taggtcttgt
     1261 gcaggcttct caccgtctcg agcacgccgt cgatgcggta gcggatgcgc atgaaggcgt
     1321 attccggctc gaaatggatg tcggaagcgc cgcgcttcac cgcgtccacc agcagcgcgt
     1381 tgacgaggcg caccatcggc tgggtgtact cgtcgccgcc gccgcccacc tgcaggctct
     1441 cgtagtcgat ctccccggtc tcgatctcct tcaggatgcc gtcgaccgag agctcgtagc
     1501 cgtagaaatt atcgatgtag tcgccgagct gcgcctcggc cgcgagcagc gtcttgatct
     1561 cgacctgcgc gccgagcatg ccgcgcagct ggtccatcgc gaccacgttg aagatctcgg
     1621 tggtggcgat ggtgagcacc tgctcggcgg agtcgtaggc gatcggcagc acgcggttgc
     1681 ggcgcgcgaa atcctggttg acgagcttca gggcctcggg atcggcgacc acctgcgcga
     1741 gatcgatcga ctcctggccg atggtgcgcg ccatgatgtc gcggatggcg ctttccgtga
     1801 cgaagccgag ccgcaccagc aggcggccga tcggcacgtt gttgtggcgc tgctcggcga
     1861 gcgcgatcga gagctgatcg gtggtgatca gcccctgctg caccagcaac tcgcccaggc
     1921 ggagtttctt ccgttgttcg gccattacgc tggaacccgc tcaaaaacga aaatggacag
     1981 gatttacaag attaacaaga ataaaaccgc aatcaatcct gtaaatcctg ataaggaagc
     2041 tctgcaaaaa gtctcgtgaa ctcggggtat ccagttgccc ccatcctacc ttctccctca
     2101 agggggaagg ggatacccgc ctccgcggga atgacgagaa acgacctatg cagaagttcc
     2161 tcgatccttc tattcgggta ttttgaagcg cgttctattc tacctgcgcg gcgagtttcc
     2221 cgatgcgctc ctgggcctgg gagacgttga aattcacgcg gcccctggcg gctgcgagct
     2281 gggcggcgcg ccggtagaag ccgagcgcca gcttcggctg gctgacgtgc tcgagggcga
     2341 ccgcgaggtt gtaggcgtaa tcgggattgg ccggctcgag atgatgggcc tggaagtagg
     2401 cctgctgcgc cgccgcccat tgcgactggc cggcgtacag gttgccgagc gtgaaataga
     2461 gatgcgcgga aggctcgcgc gcgatcagtt gccgcagctt cgactcggcg gcgagggggt
     2521 cggcgccccc gagcagcgcg atcagaccgc tttgcgcgag cgcgtgccgc ggctccagtt
     2581 ccaggatctg caggtaacgc cgcgaagctt cctcgacgtt gccctgccgg gacgcgatgg
     2641 cggcgaggcc gagcagcgcg tcgacgtttt tcggctcggc gtccaggagc tggccataca
     2701 ggcgctgcgc ttcctcggac cggcctgact gcagcgcggc gtacgcctgg gtgtgcagcg
     2761 gattcaaaac cggctccggc gtgctccggc tgaccgaaat agcgttgcgc ggccctgctt
     2821 cccgcgcggc cacgggtgct gctggttgcg gtttcggacg cgctgctgcg gccggcgcgg
     2881 gctccatcgc cgcttctttc aacaaggggg cgctggaaag cgggggcgcg acagtagttg
     2941 cgggcgcggc gggtgcaggc gcctgaacca acggcggaag gggtcccatg ggcgccgtta
     3001 tgggcggccg gccgatgaac aactggggat gaaatatctg caggtacacg taaatgccga
     3061 agccgatggc tgccaggccc gcgagcgttc cgaacaccat gaccgggtgc tcgcgcacgt
     3121 aggcgctcgc gccgcccgtc gccgggcggc tggccgtctg agcctgcatg agcctgctgg
     3181 cttgtgcctg ctcgggttca cgcgcggcgg gtgaggagga tgaggcggca ggtgcggctc
     3241 gtgatggccc ggcggccggg cggagtccgg aatcgggcct gggcgccgca gccgctgacg
     3301 ctgcggcggc catcggctcg agcgacagct cggatcggga tggctccgac gtgggaacgg
     3361 ccggcgcggg ggcgactgcc gattcgttgc gtgcctcccc ccggtccttt gcggcctttt
     3421 ccaaggcctt catcaacagg ctcatcgcgc ggcgccggcc ttctcgggtt gcggcgggga
     3481 ggcctcgggt tgtggcagaa agcgctggaa gaacctgagc tcatcgcttt ccagggaagg
     3541 attggcgacg acggtaggac gcaggaagat gaccagttcg ctcttggtca cgctctcatc
     3601 gcggtaccgg aatagctcac ccgcgatgcc aatctggtcg gcgcccggta tctgttcacg
     3661 atcgcgccgc acgctgtcct gcatcaagcc gcccaggatc acggtctggc cgctgccaac
     3721 ctgcagcacc gactccattt cgcgcacctg gatttccggc accgggctgt tgatcggcgt
     3781 aatgttgcag ccgagggtcg gcacagggat accgcaagga ttggcgagcg agggattggg
     3841 atcgttgacg aagcgcagta cgcgcgagat ggtcggtcgc accgtgatcg agacgcgacc
     3901 gtcttcgttg atctggggcg ttacgcccat gactacgccg accgcgacag tcttcgcggt
     3961 ggtgttgaag gtcgtgctcg ccgcgccctg cgtcgtcgtg gtgtctgccc gaatttcaaa
     4021 atagacgatg ttatcgacca ctttcagcaa cgccgtctgg ttgttcagtg ccatcagctt
     4081 ggggctcgac aacacccggg tgttgccgaa ttcctccagc aacttgacgg tcgcgctgat
     4141 attgccacca cgggtattcg ggttggagta gccgatctgg aagaaattgg cacccccttg
     4201 gtttagcccg gtgccaaaac cggtcagcag gctttggatg aacgaaaaac cgccatttaa
     4261 cgccagccgg ctccagtcaa tgccgccctg gtagttgttc gagagctgta cttccacgat
     4321 cgtcgcttcg atcagtacct ggcgctgcat ggcgctgacg atgctgtcga tgtgctgttg
     4381 caccagctgg tgctgccgtt cggtcgcgtt gatggttatc gtgccggaga ccgaattgac
     4441 caccacatcg tcgggcagca gcgaggcttg ttgctgggcg ccgctgcccg gcgcaccgga
     4501 aatcaccgag gcagccaggt taggcgcccc ctggccggcg cggctggcgg cttcaatctg
     4561 cttggtccgc agttcctgct cctgtttgac cagggcgagg cgctc
//
LOCUS       DBNGSG010000082         9503 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2235916, whole genome shotgun sequence.
ACCESSION   DBNGSG010000082 DBNGSG010000000
VERSION     DBNGSG010000082.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9503)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9503)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9503
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2235916"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..101
                     /locus_tag="AC158Q_02060"
     rRNA            <1..101
                     /locus_tag="AC158Q_02060"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            243..357
                     /gene="rrf"
                     /locus_tag="AC158Q_02065"
     rRNA            243..357
                     /gene="rrf"
                     /locus_tag="AC158Q_02065"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(685..3783)
                     /locus_tag="AC158Q_02070"
     CDS             complement(685..3783)
                     /locus_tag="AC158Q_02070"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HIR3338582.1"
                     /translation="MQPKFPAYAELHCLSNFSFLRGASHPHELVEKAHELGYSALAIT
                     DECSIAGVVRAHEAIKKLPPDASLKLIVGSEIRLDDGLKLVLLAADREGYGNLCSLIT
                     LGRTREEKGSYRLARHDLSGGLPGCLALLVPGVKLDQGQARFVAECFPHRAWIAVELF
                     CGPNDKSQLARLRELARDSGLPLVAAGDVHMHKPSRRELQDVLTAIRLGIPVTQAGHA
                     LYPNAERRLRQLVRLAQLYTPELLSESLEIAARCNFSLDSLRYEYPDEIVPPGHTPAT
                     YLRELTEQGLRERFPHGTEEKVRFQVEKELALIGELKYEPFFLTVYDVVRFARSKNIL
                     CQGRGSAANSTVCYALGITEVNPERMNVLFERFLSRERNEPPDIDVDFEHQRREEVIQ
                     YVYSKYGRDRAAIAATLITYQPRSAVRDVGKALSLPVQVDLLARSLVWWDGSGTLRER
                     LSDSGLDPDSRVIRKFVELVRVLIRFPRHLSQHVGGFVISRGPLSRLVPIENAAMPGR
                     SIIQWDKDDLETLGLLKVDVLALGMLTAIRRAFDFVDGTRLASTQMKEVLEREEDQAV
                     YGMICKADTIGAFQIESRAQMSMLPRLKPTKFYDLVIEVAIVRPGPIQGDMVHPYLKR
                     RKMDPKDIPYEKEALRPVLERTRGVPIFQEQVMHLAMVAADFTPDEADGLRRSMAAWK
                     RKGGLEKYEDKLRKGMAENGYSDAFITRICKQVQGFSDYGFPESHAASFALLAYVSSW
                     LKYHHPAAFLAALLNSQPMGFYSVSALMQDARRHGVEVRPVDVTTSDWDSTLERDDDA
                     PPAVRLGFSLAKGISEESVKRIVTARTKTPFSSIDDLARRCDLSRRDLHGLAAAGSLA
                     SLAGHRRNAHWLAAGERPSGILHEAPINETQPRLAPPSEREDMTADYASLDLTLGRHP
                     LALLRQRLQRMRFATAADLKLLRSGTLARAIGMVTGRQRPQTAKGTIFVTLEDETGYT
                     NVIVWGDLAERQQHALLGSKLLGVEGVMEKEGDVMHLVARQLTDHSLLLGPLGVKSRD
                     FH"
     gene            complement(3789..5234)
                     /locus_tag="AC158Q_02075"
     CDS             complement(3789..5234)
                     /locus_tag="AC158Q_02075"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF013015.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HIR3338583.1"
                     /translation="MLWLALRFPRLPLEIFTRGMAISGSLAVVSSSATNAEIVVCNQE
                     ARRHGIDTGMPVVMAWALASGLRVFTRDAPAEHAALERAAAWALQFTPTVSIATSTEI
                     LLEVEGSLRFFGGLNRLWTEIKRKTGATGYTVNMAGAPTPLAAQLFARAGLPPRIRHG
                     DALRLSLGQLPVSVLELSPALSDLLRDIGIRTLGECLELPRDGLARRAGRELLAQLDR
                     AFGDVPDPRPAFVPPQNFKATLSLPAPVESAEMLLFAARRLIAELCGLLAAIGKGAQR
                     LKFLLLHENGHETRLSLNLVAASRDPAHLTSVLRERLERARLPSPAITMTLESERLQP
                     LSSSNLAFLPDERQQTEALARLIERLRARLGETAVQGLDAVADHRPERAWHACEPGNG
                     DGTWITWRPSARPLWLLNTPRPLTQIRETPQHEGPLTLLTMPERIESGWWDGDDVRRD
                     YFVARDPAESLLWIYREHRANGGWYLHGYFS"
     gene            complement(5257..5934)
                     /gene="imuA"
                     /locus_tag="AC158Q_02080"
     CDS             complement(5257..5934)
                     /gene="imuA"
                     /locus_tag="AC158Q_02080"
                     /inference="COORDINATES: protein motif:HMM:NF033429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion DNA synthesis-associated protein
                     ImuA"
                     /protein_id="HIR3338584.1"
                     /translation="MSLEAVLNHPAIWRGNSCARVAIPSASTGFSELDAVLPGGGWPV
                     AVLTEIYAAHRGIGEMRLLMPVAARLTCAGRWVTLIAPPHIPYAPALAAHGLKLSRLI
                     LVRTERAEERFWACEQALRYQDCGAVLAWIDHAPERALKRLQLAAETGSAIALLFRSA
                     RVIPASPAALRLQIERSRDRTMVRVLKRRGSSLSAPIALDLRDLSPTSASESASASRS
                     RPLEMAS"
     gene            complement(5947..6546)
                     /gene="lexA"
                     /locus_tag="AC158Q_02085"
     CDS             complement(5947..6546)
                     /gene="lexA"
                     /locus_tag="AC158Q_02085"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008159191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HIR3338585.1"
                     /translation="MNELTVRQEQILRLISEQVANSGLPPTRAEICRAMGFRSPNAAE
                     EHLRALARKGAIEMLAGASRGIRLKQQPGIPVVGRVAAGSPILAEQHVEGRYRIDPAL
                     FKPRADYLLRVRGTSMRDAGILDDDLVAVHRTQHFRSGQIVVARIHDEVTVKRVRQRR
                     HMVELNPENPAFETIAIDTRRQPLAIEGVVVGVIRTGKL"
     gene            6796..7224
                     /gene="rimP"
                     /locus_tag="AC158Q_02090"
     CDS             6796..7224
                     /gene="rimP"
                     /locus_tag="AC158Q_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HIR3338586.1"
                     /translation="MDLNGLLETTLGGMGYELVGVERPAKGRLLRVYIDKPGGITVDD
                     CAAASNQVSRVLAVENVAYDRLEVSSPGLDRLLKGERDFARFAGQKVRVKARIAVEGQ
                     RNFVGVVREASAGRLQLEVDGRLLSLEIGNVETARLVPEL"
     gene            7237..8727
                     /gene="nusA"
                     /locus_tag="AC158Q_02095"
     CDS             7237..8727
                     /gene="nusA"
                     /locus_tag="AC158Q_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HIR3338587.1"
                     /translation="MSREILLLVDALGREKNVEKEIVFGALELALASATKKRFQEDVD
                     VRASVERTTGEFSFFRRWQVMNDGDTIEEPARQIALSDARKESPDIQVGAFIEKPLGG
                     LEVGRIGAQTAKQVILQKIRDAEREQILNDFLARKEHLVTGVIKRMERGNAIIESGRL
                     EAVLPRDQMIPKENLRVGDRARAFVWKIDRSARGPQLVLSRTAPEFIIRLFELEVPEL
                     EDGLLEIKAAARDPGSRAKIAVHSNDRRIDPIGTCVGMRGSRVQAVTSELAGERVDIV
                     LWAEDPAQFVINALAPAEVSKIKVDEEAHSMDIVVDEENLAQAIGRTGQNVRLASELT
                     GWELNLMTLEESQKKSEEEGTRVKTMFIEKLDVDEEVAGILVEEGFSTLEEVAYVPIS
                     EMQEIESFDEATVNELRSRARNALLTQAIASEEQVEHDIEDLMKVEGMDNDTARVLAA
                     KGVATQEALADFATDDLVELTGFNEERAKQLIMAARAPWFAEAAAS"
     gene            8741..>9503
                     /locus_tag="AC158Q_02100"
     CDS             8741..>9503
                     /locus_tag="AC158Q_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 associated
                     domain-containing protein"
                     /protein_id="HIR3338588.1"
                     /translation="MAQINVTQFAQQLGLPPALLIEQLQAAGVNKALAEDTPLTEKDK
                     TQLLEHLRKSHGAGEAKQKITLTRRQTTEIKKSDGLGKSRTIQIEVRKKRVLVKRDAA
                     EPDAQSDTVVKPVLDAEQIALREAEARQQAELAARQTEDAENKRRKKEADAVAEAEAK
                     APEEVRPAVAGAGAASVEGTLHKPMVKPEEKAQKAEKKAKKQQVREVVWKDDTVKRRS
                     IKTRGDVTGGLGWRARKARPGHATHTDEGEAQRAFA"
BASE COUNT         1896 a         2783 c         3095 g         1729 t
ORIGIN      
        1 ccctgaaggg tcgcggaaga ccaccgcgtt gataggccgg atgtgtaaga ggagtaatcc
       61 tttgagctaa ccggtactaa ttgcccgtgc ggcttgatcc tataaccgtg agcaaagcgg
      121 ctcaccggtg aatcagggtt tgccaagata caatcgactc attttcaaat ccttttactt
      181 cttccggttt cgagctccgg ccgcaggcta ttggctggtt gccgggtgct caacaagcta
      241 tgtctgacga ccatggcgag gtggaaccac cccttcccat tccgaacagg gccgtgaaac
      301 gactcagcgc cgatgatagt acggtgtatt ccgtgcgaaa gtaggacatc gtcaggcacc
      361 catgaaacgg acaagcccgg cctctgagag gtcgggcttt ttcgtttctg tgggtacacc
      421 cgaagagatg tcctgctttc gggacgacaa cgggccgaca tttgtctttt tgcgcggtag
      481 cgcattccgt tcggtcggct cgttgcggcg taggctaact cttcctcgct aaccgaggaa
      541 aagttaagcc tcgaaggggt ggccggagcc aaagtgggac atcgtcaggc accctcaaaa
      601 gcaaaagccc cgcctcgatc gaggcggggc ttttgctttg gtgtcgtccc ccggacaatt
      661 tcattgttta gcgagggcag gcttttagtg aaaatcccgc gatttcacac ccagtggacc
      721 gagcagcagc gagtgatcgg tcagctgccg tgccaccagg tgcatgacat cgccttcctt
      781 ttccatgacg ccctccacgc caagcagttt cgagccaagc agcgcgtgct gctgccgttc
      841 cgcgaggtca ccccacacga tgacgttcgt gtaaccggtt tcgtcctcga gcgtaacgaa
      901 tatcgttccc ttggcggttt gcgggcgctg tcggccggta accataccga tagcacgggc
      961 caacgtgccg ctacgcaaca gtttcagatc ggctgcagtg gcgaagcgca tgcgttgcaa
     1021 gcgttgtctc agcaatgcca gcggatgccg tcccaacgtc agatccagac ttgcgtaatc
     1081 cgccgtcatg tcttcccttt ccgaaggagg tgcaaggcgg ggttgcgttt cgttgatggg
     1141 tgcttcatgc aggataccgg aaggcctctc accggccgcc aaccaatggg cattccgccg
     1201 gtgcccggcg agcgaagcga gagagcctgc ggcagccagg ccgtgcagat cgcgtcggga
     1261 cagatcgcag cgccgggcga gatcgtcgat gctgctgaaa ggagtcttcg tgcgtgctgt
     1321 gacgatgcgt tttacgcttt cttcggatat gcctttggcc agggaaaatc cgagccgcac
     1381 ggcgggaggc gcgtcgtcgt cgcgttcaag tgtgctatcc cagtcgctcg ttgtcacgtc
     1441 caccgggcgc acttcgacgc catgccggcg cgcgtcctgc atcaaggccg agacggaata
     1501 gaaacccatc ggctggctgt tgagcagggc ggccagaaaa gcggcaggat ggtggtactt
     1561 gagccaggaa gacacgtaag caagcagagc gaagctcgcc gcgtgggatt ccgggaagcc
     1621 atagtcgcta aatccttgaa cttgcttaca aattcttgtt atgaatgcat ccgaatagcc
     1681 gttctccgcc atcccttttc tgagcttgtc ttcatatttt tccagtccgc ctttgcgctt
     1741 ccatgccgcc atggagcgcc gcaagccatc cgcttcatcc ggcgtgaaat cggcggccac
     1801 catggcgagg tgcatgacct gttcctggaa aatgggcacg cctcgcgtgc gctcgagcac
     1861 ggggcgcagc gcctcttttt cgtaggggat gtccttggga tccattttcc tgcgcttcag
     1921 gtagggatga accatgtcgc cctggattgg gccgggacgc acgatagcga cttcgatgac
     1981 gagatcgtag aacttcgtcg gcttgagacg aggcagcatg gacatctgcg cgcgcgattc
     2041 gatctgaaac gcgccgatgg tgtccgcttt gcagatcatc ccgtacaccg cctgatcttc
     2101 ttcccgctcg agaacctcct tcatctgggt ggatgccagg cgggtcccgt ccacgaagtc
     2161 gaacgcgcgc cggattgccg tgagcatccc gagagccagc acgtcgacct tcagcagccc
     2221 gagcgtctcc aagtcgtcct tgtcccattg gatgatgctg cgcccgggca tggcggcgtt
     2281 ctcgatgggg accagccgcg acagcggacc gcgtgaaatg acgaatccgc cgacgtgttg
     2341 cgacaagtgg cgcggaaatc tgatcagcac acggaccagc tcgacgaatt tccggattac
     2401 cctgctgtcg ggatcgaggc cggagtcgct cagacgttcc cgtagcgtgc ctgatccatc
     2461 ccaccacacc aaggacctgg ccaggaggtc tacctggacg ggcaggctca gggcttttcc
     2521 aacgtcgcgc acggcgctgc gcggctggta ggtaatgaga gtggcggcga tcgcggcgcg
     2581 gtcgcggccg tatttgctat aaacgtactg gataacttct tcgcgccgct gatgttcaaa
     2641 atcgacatcg atgtcgggcg gctcgttccg ctcgcgcgag aggaaccgct cgaacagtac
     2701 gttcatgcgc tcgggattca cttccgtaat gccgagtgca tagcagaccg tcgagtttgc
     2761 cgccgagcct cgcccctggc acagaatgtt cttgctcctg gcgaatcgca ccacgtcgta
     2821 tacggtcagg aagaagggct cgtacttcag ctcgccgata agagccagct ccttttccac
     2881 ctggaagcgc actttttctt ccgtcccatg cggaaagcgc tcccgcaggc cctgttcggt
     2941 caattcccgc agataggtcg ccggcgtatg cccgggagga acgatttcat cggggtattc
     3001 gtagcgcagg ctgtcgagcg agaagttgca gcgggcggca atctccaagg attcggagag
     3061 cagctccggc gtatatagct gcgccagtcg caccagctgg cgcaaacgcc gttcggcatt
     3121 gggatagagc gcatgacctg cctgcgtgac gggaatgccc aggcggattg ccgtcagtac
     3181 gtcctgcagt tctcggcgcg acggtttgtg catgtgcaca tcgccggctg ctacgagggg
     3241 cagcccggaa tccctggcca gctctcgcaa ccgtgccaat tgcgacttgt cgttcggccc
     3301 gcaaaacaat tcgacggcga tccatgcccg gtgcggaaag cattcggcca caaagcgtgc
     3361 ctgcccttga tcgagcttta ccccgggcac cagcagcgca aggcaacctg gcagccctcc
     3421 gcttagatca tgacgagcca gtcgatagct gcctttttct tcccgcgtgc ggccgagagt
     3481 gatcagcgag cacaaattgc cgtagccttc gcgatcggcg gccagcagca cgagcttgag
     3541 gccgtcatcg agccggattt cgcttccgac tatgagtttg agcgaagcat caggcggaag
     3601 ctttttgatc gcttcatggg cgcgcacgac gccggcgatc gagcattcgt cggtgatggc
     3661 cagcgcgctg tatcccagct cgtgcgcttt ttcgaccagc tcgtgcggat gggaggcgcc
     3721 gcgcagaaag ctgaagttgg aaaggcagtg cagctccgca taagcgggga atttcggctg
     3781 catggaactc aggaaaagta gccgtgcagg taccagccgc cgtttgcacg atgttcgcgg
     3841 taaatccaga gcaaggactc ggccgggtcg cgggcaacga aataatcccg ccggacgtca
     3901 tcgccgtccc accagcccga ttcgatgcgc tcgggcatgg tgagcagtgt cagcgggccc
     3961 tcgtgttgcg gtgtttcccg gatctgggtc aggggccgtg gcgtattcag caaccagagg
     4021 ggacgagccg atggtcgcca ggttatccat gtgccgtcgc cattgcccgg ttcgcacgca
     4081 tgccaggcgc gttccggccg atgatccgcg acggcgtcga gaccctgcac cgcggtttcc
     4141 ccgagccgtg cgcgcaagcg ctcgatgagc cgggccaaag cttcggtctg ctggcgctca
     4201 tccggcagga acgcgagatt gctcgaagag agcggctgga gcctctcgct ttccagcgtc
     4261 atcgtgatcg ccggggaggg cagcctggcg cgttccaggc gttctcgcaa cacgcttgtc
     4321 aggtgcgcgg ggtcacggct cgccgccacg agattcagcg acagacgagt ctcatggccg
     4381 ttctcatgca acaacaggaa tttcagccgc tgtgctccct tgccgatcgc ggccagcagc
     4441 ccgcacaatt ctgcgatcag gcgccgggcc gcaaacagga gcatctcggc actttccacc
     4501 ggggccggca gcgacagcgt cgccttgaag ttttgcgggg gcacaaaggc tgggcgaggg
     4561 tccgggacat ctccgaatgc gcgatcgagt tgcgcgagta attccctgcc ggcccgccgt
     4621 gccaggccgt cccttggcaa ttcgaggcat tccccgagcg ttcgaatgcc gatgtcccgt
     4681 agcaagtcgc tcaatgcagg agaaagctcg agcacgctta ccggcaactg gccgaggctc
     4741 agccgcaatg catcgccatg acggatccgc ggcggcaggc ctgcgcgtgc gaaaagctgc
     4801 gcggctaggg gagtgggtgc accggccatg ttgaccgtgt agcccgttgc gccagttttc
     4861 cgcttgattt ccgtccacag gcggttcaag ccaccgaaga atctgagcga gccttccact
     4921 tcgagcagga tctcggtgga tgtggcgatg ctgaccgtcg gagtgaattg aagtgcccat
     4981 gcagcagcgc gctccagcgc ggcgtgttcc gccggcgcgt cacgcgtgaa tactcgaagg
     5041 ccagaggcaa gtgcccaggc catcacaacc ggcatgccgg tgtcgatgcc atgccggcgc
     5101 gcttcctggt tgcaaacgac gatttcggca ttggtcgcgg aagaggagac gacggcaagc
     5161 gagcccgata ttgccatccc gcgcgtgaaa atttccaacg gcagccgagg aaaacgaagt
     5221 gcgagccaca gcatgactgc ttccgtcagg ccgactttac gacgccattt ccagtgggcg
     5281 ggaacggctg gccgaagctg actcgctggc tgaggttgga gacaaatccc gcaagtcgag
     5341 tgcgataggc gcggacagac tgctgccgcg gcgcttaagt acccgcacca tggttcgatc
     5401 ccgagagcgc tctatctgca gccgcagcgc ggcgggcgaa gccgggatca cgcgggctga
     5461 acggaacagc agggcgatcg cgctaccggt ttcagccgcc agttgcagac gcttgagcgc
     5521 gcgctcgggt gcatgatcga tccatgcaag gacggcgcca cagtcctgat aacgcagcgc
     5581 ctgctcgcac gcccagaatc gttcctcggc tctctcggtg cgtaccagta tcaggcgcga
     5641 gagcttcagc ccgtgcgcgg cgagcgccgg ggcgtaggga atgtgcggcg gagcaatcag
     5701 tgtgacccag cgcccggcac aggtgaggcg cgcggcgaca ggcatgagga gccgcatttc
     5761 gccgatgccc cggtgcgcgg catagatctc agtcaggacc gccactggcc agccgccacc
     5821 aggaagcacc gcatccaatt ctgaaaagcc ggtagaagcg ctgggaattg ctaccctggc
     5881 gcagctattt ccacgccaga tagcaggatg attgagcaca gcttcgaggg acatacggcg
     5941 gcgctttcag agcttgccgg tgcggataac ccctacgacg acgccttcga tggcgagtgg
     6001 ctggcggcgc gtatctatcg cgatggtttc gaaggcggga ttttccggat tgagctcgac
     6061 catgtgccgg cgctggcgga cgcgcttgac cgtgacttcg tcgtggatgc gcgccaccac
     6121 gatctggccg gagcggaagt gctgggtgcg atgcactgcc accagatcat cgtcgagtat
     6181 tcctgcatcc cgcatgcttg tgccccgcac tcgcagcagg tagtcggcgc gcggcttgaa
     6241 cagggcggga tcgatacggt aacggccctc cacatgctgc tcggcgagga tagggctgcc
     6301 ggcggcgacc cggcccacga ccggtattcc cggctgctgc ttcagccgaa ttccgcggga
     6361 agcgcccgcc agcatctcga tcgcgccctt gcgcgcgagc gcgcgcaagt gttcctcggc
     6421 ggcattaggg gagcgaaagc ccatcgcacg gcaaatctcg gcccgtgttg gtggcaggcc
     6481 cgagttggca acttgctcgc tgatgaggcg caggatttgt tcctggcgga cggtgagttc
     6541 gttcatggtg aatactgtaa gtatatacag tatatggcct gggacaagag cagcaggagc
     6601 gactgcgttg acttgacccg cggctgcggc gcaggagagc ggaaataggc gtgggcgcag
     6661 tcggccgtga ctgggccggt ggtgataact cttggattta agcgcgattt ttgttacact
     6721 cctcaccccc tgattccggg ggaaaacgaa agtgggctgg ccaagcccat tttttgtttg
     6781 tagagcggat tttcgatgga tttgaacggg ttactggaga cgacgttggg cggcatgggt
     6841 tacgaactgg tcggcgtaga gcggcccgcc aagggacggc tgttgcgcgt ttacatcgac
     6901 aagcctggag gcattaccgt agacgactgc gccgcggcga gcaaccaggt gagccgggtg
     6961 ctggcggtgg aaaacgtggc ttacgaccgc ttggaagtgt cgtcgccggg gctggaccgg
     7021 ctgttgaagg gcgaacgcga tttcgcgcgc tttgccgggc agaaagtgcg cgtcaaggcg
     7081 cgcattgcgg tggaggggca gcgcaatttc gttggtgtgg tgcgcgaagc cagcgccggg
     7141 cgcctgcaac tcgaagtgga cggcaggctg ctgtcgctcg agattggtaa cgtggaaacg
     7201 gcgcgcttgg tgccggagtt atagcaggag acagcgatga gccgggaaat tctgttgctg
     7261 gtggacgccc tgggacggga aaagaacgtc gaaaaagaga tcgtgttcgg cgcgcttgaa
     7321 ctcgccctgg cttcagccac caagaagcgt tttcaagagg acgtcgacgt gcgtgcctcg
     7381 gtggagcgca cgacggggga attctcgttc tttcgccgat ggcaggtgat gaacgatgga
     7441 gataccatcg aggaaccggc gcgccagatt gcactgagtg acgcacgcaa ggagagcccc
     7501 gatatccagg tgggggcatt catcgaaaaa ccgctcggtg gcctggaagt cgggcgtatc
     7561 ggcgcgcaga cggcgaaaca ggtcatcctc cagaagatcc gcgacgccga gcgtgagcag
     7621 attctcaacg attttctggc gcgcaaggag catctcgtca ccggggtgat caagcgcatg
     7681 gagcgtggca acgccatcat cgagtcgggg cggctggagg ccgtgctgcc gcgcgaccag
     7741 atgattccca aggaaaacct gcgcgtcggc gaccgcgcgc gggcctttgt ctggaaaatc
     7801 gaccgctcgg ccagggggcc ccagcttgtg ctctcgcgca cggccccgga gttcatcatc
     7861 aggctgtttg agctcgaagt gcctgaactc gaggacggac tgcttgagat caaggcagcg
     7921 gccagggacc ccggctcgcg cgcgaaaatt gctgttcatt ccaatgatcg ccggatcgat
     7981 ccgatcggca cctgcgtcgg catgcgtggc tcgcgcgtcc aggcggtgac ttccgaactc
     8041 gccggcgaac gcgtggacat cgtgctgtgg gcagaggacc cggctcagtt cgtgatcaac
     8101 gccctcgcgc cggcagaggt cagcaagatc aaagtggacg aggaagcgca cagcatggac
     8161 atcgtggtgg acgaggaaaa cctcgcccag gcgatcgggc gtaccggcca gaacgtgcgc
     8221 ctggcgagcg agttgaccgg ctgggaactt aacctcatga cgctggagga gtcccagaag
     8281 aagagcgagg aggagggtac gcgcgtcaag acgatgttca tagaaaaact cgacgttgac
     8341 gaagaggtcg cgggcatcct ggtggaggaa ggcttcagta ccctcgaaga agtcgcctac
     8401 gtcccaatca gcgagatgca ggagattgaa tctttcgacg aagccaccgt gaatgaatta
     8461 aggagccgcg cccgcaatgc gctgctgacc caggcgatag cgagcgagga gcaggtcgag
     8521 cacgatatcg aggacctgat gaaagtcgaa ggcatggaca acgatactgc gcgcgtactc
     8581 gcagccaagg gggtggccac gcaggaggcg cttgccgatt tcgcgacaga cgacctggtg
     8641 gaattgacgg gattcaatga agaacgcgcc aagcagctca tcatggctgc tcgcgccccc
     8701 tggtttgccg aagccgccgc ttcctgagac ccactgaccg atggcgcaga tcaacgtcac
     8761 tcaattcgcc cagcagctgg gattgccgcc ggcgctgctc atcgagcagt tgcaggccgc
     8821 tggcgtgaac aaggccctcg cggaggacac cccgcttacc gagaaggaca agacccagtt
     8881 gctcgagcat ttgcgcaagt cgcatggcgc gggcgaggcg aagcagaaaa taacgctcac
     8941 ccggcgccag accacggaga tcaagaaatc cgatggacta ggcaagtcgc gcaccatcca
     9001 gatcgaggtg cgcaagaagc gggtattggt gaaacgcgac gcggccgagc ccgatgcgca
     9061 gtcagacacg gtggtcaaac ccgtgctcga cgcagagcag atcgcgttgc gcgaagccga
     9121 ggccaggcag caggccgagc ttgcggcgcg gcaaacggag gacgcagaga acaagcggcg
     9181 caagaaggaa gcggacgcgg tagcagaagc ggaagccaag gcgccggagg aagtcagacc
     9241 ggcggtggcg ggcgcagggg ccgcaagcgt ggagggcacg ctgcacaagc cgatggtcaa
     9301 gcccgaggaa aaggcccaga aagccgagaa gaaggccaaa aagcagcagg tcagggaagt
     9361 cgtctggaag gatgacacag tcaagcgccg gagcatcaag acgcgcggcg atgtgaccgg
     9421 tggactgggc tggcgcgcgc gaaaggcccg tcccggccac gcaacccaca cggatgaagg
     9481 cgaggcgcag cgcgcgtttg cgg
//
LOCUS       DBNGSG010000083         4126 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3933603, whole genome shotgun sequence.
ACCESSION   DBNGSG010000083 DBNGSG010000000
VERSION     DBNGSG010000083.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4126)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3933603"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="AC158Q_02105"
     CDS             <1..325
                     /locus_tag="AC158Q_02105"
                     /inference="COORDINATES: protein motif:HMM:NF017015.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR3338589.1"
                     /translation="EGREHGYDARNTKDHPPLADLWRAQQEIDRWYMAWSDAVSDAAL
                     DEKVSFTLIGGNRGVMTRGEILLHVVNHTTYHRGFVGDLFYQVPARPPTTDLPVYLRE
                     QGEAG"
     gene            418..846
                     /locus_tag="AC158Q_02110"
     CDS             418..846
                     /locus_tag="AC158Q_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HIR3338590.1"
                     /translation="MIDWAHFTPWSALAGGVIIGAAAALFLLLNGRIAGISGILGGLL
                     RPAPGDAVWRAAFIGGLIFSPALYALFSGVPDATIQAGYPVLVLAGLLVGAGTRYGSG
                     CTSGHGVCGLSRLSPRSLVATAAFMGAGFATVFAVRHLLA"
     gene            857..1285
                     /locus_tag="AC158Q_02115"
     CDS             857..1285
                     /locus_tag="AC158Q_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HIR3338591.1"
                     /translation="MHSLSAFLIGTLFGAGLIVSGMTDPSKVIGFLDLAGAWDPSLTF
                     VMGGAVIVGLIAFRVARKRTAAFLGGAMHVPAARHIDRRLVLGSLVFGAGWGLAGFCP
                     GPALVALGAGYDKAVVFVLAMLAGMALFEISERFLHGRMK"
     gene            1282..2535
                     /locus_tag="AC158Q_02120"
     CDS             1282..2535
                     /locus_tag="AC158Q_02120"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3338592.1"
                     /translation="MTEATVPAPAERAAQASFAALRHRGFRVYFVTTALAMAADQVEH
                     VISYWVLFEKFQSPALAGFAVLSHWLPFLFFSVYFGAMADRFDSRRIIQIAMLMFMAV
                     SFSWAVLFYTDTIEVWHAMVLLVVHGMAGVLWGPASQVLIHDIVGRENLVSAVRLNAT
                     GRQLGVFLGPGIGGALMLLIGPEAGLIVNVLIYLPLAWWLWRAPYAAEHRRAAATVHS
                     GSFADAVKVLREVSGNRTLISMIVLAGVTSLFVGNAFLAQMPEYAHDLGTEKADFSYA
                     VLLGANAAGAFIGGIILESRGLLRSTPQTAIVLTLLWCLSIAGFAATDSYPVAVTLMF
                     VAGFLSLAYNSMAQALVQLESPAHLRGRLIGLFQMSNNGLRAFSGITVGLVGSLIGIH
                     WSLALSALALLAVTFVLLAFAMRTR"
     gene            2543..3751
                     /locus_tag="AC158Q_02125"
     CDS             2543..3751
                     /locus_tag="AC158Q_02125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3338593.1"
                     /translation="MAVKSQVIVAGAGPVGLLTALVLARQGVPVLVLEAEPGLTVDLR
                     AGTFHPPSLEMMAPFGITDEMLKTAIPVPRWQIRDKRDGVIVEWDVSLIRDLTPYPYR
                     LHLEQHRLTPIIHSKLRECPNAGIRFAHELTGFTQDADKVVVTARTPGGTERFESQWL
                     VGADGGRSTVRKGMGVAFDGYTWPERFVVASTVYDFAPHGYTMNAYVADPVEWSAMFK
                     MPDDGPPGMWRVLWPIPPQEDEAVALSEESIESHMQRFQPKAGRYPIKYRSVYKVHQR
                     VAGDFRAGRALLAGDAAHLNNPIGAFGLNGGIHDAINLAGKLGKVCRGEADASLLDLY
                     VRQRRTVNLEYVQEYSVRNLRRLAARTEEERRKNFDELRRAASTPEGVRDFLLISSMI
                     ASVRRADAIT"
     gene            3890..>4126
                     /locus_tag="AC158Q_02130"
     CDS             3890..>4126
                     /locus_tag="AC158Q_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DODA-type extradiol aromatic ring-opening family
                     dioxygenase"
                     /protein_id="HIR3338594.1"
                     /translation="MARIIGGIGTSHVPTIAMAYDRKKQNDPDWAPLFKGYEPVTKWM
                     ADRKPDVLVFFYNDHATTFLFDHYPTFAVGVGAEY"
BASE COUNT          609 a         1323 c         1447 g          747 t
ORIGIN      
        1 cgagggacgc gagcacggtt acgatgcgcg caacacgaag gaccatccgc cgctcgcgga
       61 cctttggcgc gcgcagcagg agatcgaccg gtggtacatg gcgtggagcg acgcggtctc
      121 ggacgcggcg ctggacgaga aggtgagctt cacgctgatt ggcggcaatc gcggcgtgat
      181 gacgcgcggc gagatcctgc tgcacgtcgt gaaccacacg acctaccacc gcggtttcgt
      241 cggcgacctt ttctaccagg tgccggcgcg gccgcccacc accgaccttc ccgtgtacct
      301 ccgggaacag ggagaggcgg gataagggat gggggatagg ggaattggat ttgtttccgc
      361 gcgagcgcgg gtagcttatc ccttatcccg tctcctctgt atcacgcggt ttcgcagatg
      421 atcgactggg cccatttcac cccctggagc gcgctggccg gcggcgtcat catcggcgcg
      481 gcggcggcgc tgttcctgct gctgaacggc cggatcgccg gcatcagcgg catactgggc
      541 gggctgctgc ggcccgcgcc gggcgacgcc gtgtggcgag ccgcgttcat cggcggcctg
      601 attttttccc cggcgctgta cgcgttgttt tcaggagtgc cggacgcgac gatccaggcg
      661 ggctatccgg tgctggtgct ggccgggctg ctggtgggcg cggggacgcg ctacggatca
      721 ggctgcacca gcgggcacgg tgtctgcggg ttgtcgcggc tttctccccg ttcgctggtc
      781 gcgaccgcgg cgttcatggg ggcggggttc gccacggttt tcgcggttcg ccacctgctg
      841 gcgtgaggcg aaagccatgc acagtttgag cgcatttctt atcggtaccc tgttcggggc
      901 cggcctgatc gtgtcaggca tgaccgatcc gtccaaggtg atcggctttc tcgacctggc
      961 cggggcctgg gatccctcgc tgaccttcgt catgggcggg gcggtgatcg tcggattgat
     1021 cgcatttcgc gtggcacgca agcgcacggc ggccttcctg ggcggagcga tgcacgtgcc
     1081 cgcggcccgg catatcgacc gccggctcgt actcggcagc ctggtgttcg gcgccggctg
     1141 gggactcgcc ggtttctgcc cggggcccgc actggtcgcg ctgggcgccg gatacgacaa
     1201 ggcggtggtg ttcgtgctcg ccatgctggc cgggatggcg ctgttcgaga tctcggagcg
     1261 attcctgcac ggcaggatga agtgaccgag gccaccgttc ccgccccggc cgagcgggcc
     1321 gcgcaggcct cgttcgcggc gttgcgccac cgcggcttcc gcgtctattt cgtgacgacg
     1381 gcgctcgcga tggcggccga ccaggtcgag cacgtcatca gctattgggt gctgttcgag
     1441 aaattccagt cgccggccct cgcggggttc gcggtgctgt cgcactggct gccgttcctc
     1501 ttcttctcgg tctacttcgg ggcgatggcc gaccgcttcg acagccgccg catcatccag
     1561 atcgcgatgc tcatgttcat ggccgtctcg ttctcgtggg cggttctgtt ctacaccgac
     1621 accatcgagg tctggcacgc gatggtgctg ctcgtcgtcc atggcatggc gggcgtgttg
     1681 tgggggccgg cgagccaggt gctgatccac gatatcgtcg ggcgcgagaa tctggtgagc
     1741 gcggtgcggc tgaacgcgac cggccgccag ctgggcgtgt tcctgggccc gggaatcggc
     1801 ggcgcgctca tgctgctgat cggcccggag gccggcctca tcgtgaacgt gctcatctac
     1861 ctgccgctgg cctggtggct gtggcgggcg ccgtacgcgg ccgagcaccg gcgcgccgcc
     1921 gcgacggtcc attccggcag tttcgccgat gccgttaaag tcctgcgcga ggtttccggc
     1981 aaccgcactc tcatctcgat gatcgtgctg gcgggggtga cctcgctgtt cgtcggcaac
     2041 gcgttcctcg cgcagatgcc ggagtacgcc cacgatcttg gcacggagaa ggccgatttc
     2101 tcctacgccg tgctgctcgg cgccaacgcc gccggcgcgt tcatcggcgg catcatcctc
     2161 gagtcgaggg gcttgctgcg ttccacgccc cagaccgcga tcgtcctcac gctgctgtgg
     2221 tgcctgagca tcgccggttt cgccgccacg gacagctacc cggtcgcggt cacgctcatg
     2281 ttcgtcgccg gctttctctc cctcgcgtac aactccatgg cccaggcctt ggtccagctc
     2341 gaatcgccgg cacacctgcg cggccggctg atcgggctgt tccagatgtc caacaacggc
     2401 ctgcgcgcgt tcagcggcat cacggtgggg ctggtcggca gcctgatcgg gatccactgg
     2461 tcgctcgcgc tctccgcgct ggcgctgctg gcggtgacgt tcgtgctgct ggccttcgcg
     2521 atgcgaacac gctgaggcgg gaatggcggt gaaatcgcag gtcatcgttg cgggcgcggg
     2581 accggtcggt ctgctgacgg cgctcgtgct cgccagacag ggcgttcccg tgctggtgct
     2641 cgaagccgag ccgggcctca ccgtggattt gcgcgcgggc acgttccacc cgccctcgct
     2701 cgagatgatg gcgccgttcg gcatcacgga cgagatgctg aagaccgcga tcccggtgcc
     2761 gcgctggcag atccgcgaca agcgggacgg cgtgatcgtc gagtgggacg tctcgctcat
     2821 ccgcgacctg acgccctatc cctaccggct gcacctggag cagcaccggc tgacgccgat
     2881 tatccattcg aaactgcggg aatgcccgaa tgccgggata cgattcgcgc acgagctgac
     2941 cggtttcacg caggacgccg acaaagtggt ggtgacggct agaacgccgg gcgggacgga
     3001 gcgcttcgaa tcgcagtggc tggtcggcgc ggatggcggg cgcagcacgg tgagaaaggg
     3061 catgggcgtg gcattcgacg gctacacctg gccggagcgc ttcgtcgtgg cgagcacggt
     3121 ctacgatttc gccccgcacg gctacaccat gaatgcctac gtcgccgacc cggtggagtg
     3181 gagcgcgatg ttcaagatgc ccgacgacgg cccgcccggg atgtggcgcg tgctgtggcc
     3241 gattccgccg caggaggacg aggcggtggc gctgtcggaa gagagcatcg agagccacat
     3301 gcagcgcttt cagccgaagg cggggcgcta cccgatcaag taccgcagcg tgtacaaggt
     3361 gcaccagcgc gtggcgggcg atttccgcgc cgggcgcgcg ctgctggcgg gagacgccgc
     3421 gcatctcaac aacccgatcg gcgcgttcgg cctgaacggc ggcatccacg acgcgatcaa
     3481 cctggccggg aaactcggca aggtctgccg cggcgaggcc gatgcctcgc tcctcgatct
     3541 ctacgtgcgc cagcgccgca cggtgaacct ggaatacgtg caggaatact cggtgcgcaa
     3601 cctcaggcgg ctggccgcgc ggaccgagga agaacgccgg aagaacttcg acgagctgcg
     3661 ccgggccgcg tccacgccgg agggggtgcg cgacttcctg ctcatctcca gcatgatcgc
     3721 cagcgtgcgc agggcggacg ccatcacgtg agggatgagg gatgagggca gagggatgag
     3781 gagttaaggc gaaaaaaagg gtctaactgc ggatcacgga tcggggatca cgccttctgg
     3841 ccattgaggc atactatccg cgacggcgct tggcatttcc ggggtattca tggcacgaat
     3901 catcggcggc atcggcactt cccacgtccc cacgatcgcg atggcgtacg accggaagaa
     3961 gcagaacgac ccggactggg cgccgctgtt caagggctac gagccggtta cgaagtggat
     4021 ggctgaccgc aagcccgacg tgctcgtgtt cttctacaac gaccacgcga cgacgtttct
     4081 gttcgaccac tatcccacct tcgcggtggg cgtcggcgcc gagtat
//
LOCUS       DBNGSG010000084         3567 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1557050, whole genome shotgun sequence.
ACCESSION   DBNGSG010000084 DBNGSG010000000
VERSION     DBNGSG010000084.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1557050"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="AC158Q_02135"
     CDS             <1..468
                     /locus_tag="AC158Q_02135"
                     /inference="COORDINATES: protein motif:HMM:NF024490.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HIR3338595.1"
                     /translation="IAKMNGLVDRCLIEELYGASEAGVRIDLIVRGICCLRPAVPGLS
                     SRIRVISIVDRYLEHARISYFENGADAEYLLASADWMPRNLDRRVEIAFPVLDPHLQT
                     QIREILEIQLADTVKARRILPDGSSARIRGQGESRLRSQERLYETLATNGFFR"
     gene            737..1759
                     /locus_tag="AC158Q_02140"
     CDS             737..1759
                     /locus_tag="AC158Q_02140"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR3338596.1"
                     /translation="MQFGLFGSAAARRGGAEFDSSEGFRDFIEYNIEAEALGFHSTFV
                     VEHHFTGYGQVSATLNLLTWLGARTRRLRLGTAVIVLPWHNPVLLAEQAATLDLLSGG
                     RLDFGIGKGYRYNEFAGFRVPMEEADARFEECLEVLLKAWTADGPFSHRGKYWEFENI
                     VVEPPPAQKPHPQVWMGAGSERSIRQVAAQGFNLLLGQYASPTDVASHIAAYRSEVEA
                     RGRRFDPMQVGVTRAFFVTDLAEERDAALERRLQNRVRQLKLATRPDGTVHGGPDRAT
                     GDPRTINEKSAMYGSPDDIAKKLEELRDVGVGYVLINGGGSGGGARGRESLRRFARDV
                     MPIFAD"
     gene            complement(1903..2322)
                     /locus_tag="AC158Q_02145"
     CDS             complement(1903..2322)
                     /locus_tag="AC158Q_02145"
                     /inference="COORDINATES: protein motif:HMM:NF013920.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HIR3338597.1"
                     /translation="MAIARPVRAMDPYAEQFWAFTRERELRLQQWAACGKFRWPPGPT
                     CDRCLSDDFAWSALSGVAKVLSWTTFHRGYFPEYPPPHTVVALELAEGPLFISYPVGL
                     EPSELREGMTLEVRWTDAADRFGEYNLPVFGPPAARG"
     gene            complement(2325..>3567)
                     /locus_tag="AC158Q_02150"
     CDS             complement(2325..>3567)
                     /locus_tag="AC158Q_02150"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR3338598.1"
                     /translation="VAPLDNGREEEAAMAVSFDGARLGRLKDTVAVVGVGETDYAKDY
                     RAPGGQAVGKGQARYDSYTLASRALKRALDDAGLRKDDIDGLCTSGTLSGERASELWG
                     LNPRWSGGGDAAQCIIEATLAIDAGLCTTVAMVYGNAQRSMDTAYGGARVTGGAITSY
                     FYYAPWGMTSQGALYALFFQRHKLLYRTTEEQLGAVAVAFRKHACLNDNAVMQKPLTI
                     QDYLSAPYIAEPLRLYDYCLINDGGVAIIVRRADMARDLRQRPIMVSGFGWSEENIDA
                     TQLRPRLKTFYHGAHHDVAAQVYPMAGVTPRDVDVFATYDSFSVHLLASLEGFGFCRE
                     GEAGPFVQGGRIEIGGDLPCNTSGGMLSESYMQSWNHQPELVRQLRGGLDKRQVEGAE
                     VAQYVHDVAGKCKSLIYTKGA"
BASE COUNT          574 a         1199 c         1204 g          590 t
ORIGIN      
        1 atcgcgaaga tgaacggcct cgtagatcgc tgcctgatcg aggagctgta cggcgccagc
       61 gaagcgggcg tgcgtatcga cctgatcgtg cgcggcatct gttgcctgcg ccccgcggtg
      121 cccgggctct cgtcgaggat ccgcgtcatc tccatcgtcg accgttacct cgagcacgcg
      181 cgcatctcct acttcgagaa cggtgcggac gcggaatacc tgctcgcctc cgcggactgg
      241 atgccccgca atctcgaccg tcgcgtcgag atcgcgttcc cggtgctgga tccccacctc
      301 cagacccaga tccgcgagat cctcgagatc cagctcgccg acaccgtgaa ggcccggcgc
      361 atcctccccg acggaagctc cgcacgtatc cgtgggcaag gcgagtctcg gctccgctcg
      421 caggagcgtc tctacgagac gctggcgacg aatgggttct tccggtagcg cggagagggt
      481 cgagcacggc tcgttccctc ctcgcgcggc caagattcag agccttctgt tatcaggagt
      541 atttcttgtg cgtggcggcg cggaaccgca gcggggcacg atgagcagca cgcagccgaa
      601 atcgcgagag cagctggtat gatccggccc tgggagaacg cccgttgagg taggcagccc
      661 tgggcagcgc ggctgtcatg acgcacatcg ccggccaggt cgccacctca ttgcgccgta
      721 atggagggtg agacgaatgc aattcggcct gttcggtagt gcggcggcga gacgcggtgg
      781 tgcggaattc gacagcagcg agggatttcg tgatttcatc gaatacaaca tcgaggctga
      841 agcgcttggc tttcacagca cgttcgtcgt cgagcaccac ttcacgggct acggtcaagt
      901 ttcggccacg ctcaatctcc tgacctggct tggggcgcgc acgcgccggc tgcgtctcgg
      961 caccgcggtc atcgtgctgc catggcacaa cccggtgctg ctggcggaac aggccgcgac
     1021 cctcgatctt ctgtccgggg gccggctcga tttcggtatc ggcaagggct atcggtacaa
     1081 cgagttcgca ggtttccgcg tgccgatgga agaagccgac gcgaggttcg aggagtgcct
     1141 cgaggttctc ctgaaagcgt ggaccgccga cgggcccttt tcccatcggg gcaagtactg
     1201 ggagttcgag aacatcgtgg tcgaaccgcc gccagcacag aaaccgcacc cccaagtctg
     1261 gatgggcgcg ggcagtgagc gctcgatccg ccaggtcgcc gcccaaggct tcaacctgct
     1321 cctcgggcaa tacgcttcgc ccacggacgt ggcctcgcac attgccgcct acagatccga
     1381 ggtggaggct cgtggccgcc gtttcgaccc gatgcaggtc ggggtaaccc gagccttttt
     1441 cgtgaccgac cttgctgagg aacgagacgc ggcactggag cgccggctgc agaatcgggt
     1501 ccgccaactg aagctcgcca cccggcccga cggcacggtc cacggcggcc cggaccgggc
     1561 caccggcgat ccccggacca tcaacgagaa gagcgccatg tacggcagcc ccgacgacat
     1621 cgcgaagaaa ctcgaggagc tgcgcgacgt cggggtgggc tatgtcctga tcaacggcgg
     1681 cggttcgggt ggtggcgctc gcgggcgcga aagcctgcgc cgcttcgccc gcgacgtcat
     1741 gccgatattt gccgactgag agccttcgct cctcgcgtga ggcattgctc gggctgcttc
     1801 atcgcaacca cgagcgggga gggcgacggc gcacgctcaa ttgcgggaac gtcctccaga
     1861 acgtgatcga gccgtgcacg ccagccgtgg ccgccggtgg cctcaaccgc gcgcggcggg
     1921 cggaccgaag accggcaggt tgtactcgcc gaaccggtcg gcggcgtccg tccaccgaac
     1981 ctccaaggtc atgccctccc gcaactcgct cggctcgagg ccgacgggat aggagatgaa
     2041 gagcgggccc tcggccagct cgagcgcgac gaccgtgtgg ggcggcggat actcggggaa
     2101 gtagccgcgg tggaacgtcg tccacgagag caccttggcc acgccgctga gcgccgacca
     2161 cgcgaagtcg tcggagagac agcggtcgca ggtcgggccg gggggccagc ggaacttccc
     2221 gcacgccgcc cattgctgca ggcgcagctc gcgctcccgc gtgaaggccc agaactgctc
     2281 ggcgtagggg tccatcgcgc ggacgggacg ggcgatggcc atcgtcacgc tcccttcgtg
     2341 tagatcaggc tcttgcactt gcccgcgacg tcgtggacgt actgggcgac ctcggcgcct
     2401 tccacctgcc gcttgtcgag accgccccgc agctggcgga ccagctccgg ctggtggttc
     2461 cagctctgca tgtagctctc ggacagcatg ccgccgctgg tgttgcacgg cagatcgccg
     2521 ccgatctcga tgcggcctcc ctgcacgaac gggccagcct cgccctccct gcagaagccg
     2581 aagccctcca gtgaggcgag caggtgcacg ctgaagctgt cgtaggtggc gaacacgtcg
     2641 acgtcgcgcg gggtgacgcc cgccatggga tagacctggg cggccacgtc gtggtgagcg
     2701 ccgtggtaga aggtcttgag ccgcggccgg agctgggtgg cgtcgatgtt ctcctcggac
     2761 catccgaagc cgctcaccat gatcggccgc tgcctgaggt ctctggccat gtccgcgcgg
     2821 cgcacgatga tggccacgcc gccgtcgttg atgaggcagt agtcgtagag ccggagcggc
     2881 tcggcgatat acggggcgct caggtaatcc tggatggtga gcggcttctg catcacggcg
     2941 ttgtcgttca gacaggcgtg cttgcggaag gccaccgcga ccgcgccgag ctgctcctcg
     3001 gtggtgcggt agagcagctt gtggcgctgg aagaagagcg cgtagagcgc gccctgcgac
     3061 gtcatgcccc acggggcgta gtagaagtac gaggtgatgg ctccgccggt cacccgggcg
     3121 ccgccgtagg ccgtgtccat cgagcgctgc gcgttaccgt agaccatcgc gacggtcgtg
     3181 cagagccctg cgtcgatggc aagggtcgcc tcgatgatgc attgagcggc gtcgccgcca
     3241 ccgctccagc gcgggttaag tccccacagc tcgctggccc gctcgccgga gagcgtgccg
     3301 gaggtgcaca ggccgtcgat atcgtccttc cgcaagcccg cgtcgtccag cgcccgcttg
     3361 agggccctcg acgcgagcgt gtacgagtcg taccgggcct gccccttgcc cacggcctgg
     3421 cccccgggtg cccggtagtc cttcgcgtag tccgtctcgc ccacgccgac caccgccacc
     3481 gtgtctttca gtcgcccgag tcgcgccccg tcgaagctca ccgccatcgc tgcttcctcc
     3541 tcccgaccgt tatcgagggg cgccacg
//
LOCUS       DBNGSG010000085         6657 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4443575, whole genome shotgun sequence.
ACCESSION   DBNGSG010000085 DBNGSG010000000
VERSION     DBNGSG010000085.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6657)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6657)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6657
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4443575"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1336
                     /locus_tag="AC158Q_02155"
     CDS             <1..1336
                     /locus_tag="AC158Q_02155"
                     /EC_number="2.7.7.-"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR3338599.1"
                     /translation="KQGSFTIGDQRRLLALCAQAAVSIENAQLFEEVSNSRGYNESIL
                     RSMSNGVITLDAESRVSKINESARRILKRSDDETVGRTVHEIFGDSNRWVMKSLDRVI
                     GTGMIDSTVDANLQMDGNEVVSVNLTAVPLRDMRDDRIGSMLILEDLTREKRLRNTMS
                     RYMSKAVVDQLLDKGDAVLGGTGREVSVLFSDIRGFTALSERLGARETVAMLNEYFTD
                     MVDVVYEHNGILDKYIGDMIMAVFGSVLASEEDADNAVTVGSRMLVALRDLNSRRLTG
                     GHEPIRIGVGISTGYCVAGNIGSPKRLEYTVIGDRVNLAERLQNANKYYGTSVLICQP
                     TAARLKRSVKIRELDLIRVRGINAPVSIFEVLDHHTASSFPNLDVVLPAFKEGLVHYR
                     QRSWDQAAKFFSEALSACPGDRPSRIYLERCETYRTDPPIASWDGVSVVQNI"
     gene            complement(1474..1740)
                     /locus_tag="AC158Q_02160"
     CDS             complement(1474..1740)
                     /locus_tag="AC158Q_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338600.1"
                     /translation="MTTFEQWRSPSSRMTAAGTGHKPRKYDIRVVDQFYAYCQAIAHT
                     EKHRDEARCRSHGAARRSIWTMLVTASFMFYYLMDRVAQAVSLF"
     gene            1932..2393
                     /locus_tag="AC158Q_02165"
     CDS             1932..2393
                     /locus_tag="AC158Q_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338601.1"
                     /translation="MLATAAGCAALITSVWGADDPGARALEQHQLQRQQQQEALQLRM
                     QQQQRAVQSTPADPRQKQAQEKLRADQQLQQQQLHYRQAIEPAAAQPSDDQGARRAKA
                     ERDRLEAQRQGQRQIRQSDWDLQQEVGARNEPGAAVPVPPRQLKIPAGTDR"
     gene            complement(2428..2973)
                     /locus_tag="AC158Q_02170"
     CDS             complement(2428..2973)
                     /locus_tag="AC158Q_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338602.1"
                     /translation="MSRARFRCFALAGLCLWLAAAGTVSQAGAPPGWKEVAIPAGRFK
                     VLMPGAPKTSRQTIRTDIGNVAATRYTVTDNANVTYDVLFNEYPAAGVAKVNPQKLLD
                     SARDGLLYQTKGKMVSEKHITLSGFPGREQIIMSGEGTHYRVRLAWAGNRLYQVMAVS
                     PGKPRPDAELFFDSFQIVGKR"
     gene            complement(2970..3230)
                     /locus_tag="AC158Q_02175"
     CDS             complement(2970..3230)
                     /locus_tag="AC158Q_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338603.1"
                     /translation="MPAIHVSARSLPERIVYGVLLLAVLVLGFFFLVAALIAGAILAG
                     IVLLRFWWLKRRLRKAADEEFITADYTVVERERPAAPRLPPQ"
     gene            complement(3352..4170)
                     /locus_tag="AC158Q_02180"
     CDS             complement(3352..4170)
                     /locus_tag="AC158Q_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HIR3338604.1"
                     /translation="MAQDDSNNMALFCDFENVALGVREARYAEFDVKKVLERLLLKGS
                     IVVKKAYCDWERYKDFKKAMHEAAFEMIEIPHVRMSGKNSADIRMVVDALDLCYTKSH
                     VDTFVIISGDSDFSPLVSKLRENNKTVIGVGVKKSSSDLLVANCDEFIYYDDLVREKQ
                     KRPPRRKPAAASPGAPDAPKAPATDGDKKQEALDLVMETVEALLRERGAEEKIWGSMV
                     KQALKRRQPGFNESYYGFRSFGKLLDEAETRKLLTLEHDEKSGGVIIKSFNTDS"
     gene            complement(4207..4899)
                     /gene="nfi"
                     /locus_tag="AC158Q_02185"
     CDS             complement(4207..4899)
                     /gene="nfi"
                     /locus_tag="AC158Q_02185"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: protein motif:HMM:NF008629.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HIR3338605.1"
                     /translation="MRRIRHTWNLTPRAAIALQHRLRSKVIRTGRPRQVRHVAGTDVG
                     FERGGGITRAAVAVLSFPALELVDHAIVRQPTRFPYVPGLLSFRELPALLAAFDRLRI
                     QPDLLLCDGQGLAHPRRFGLACHMGVTCGIPAIGIAKSRLLGEHGQPMQRRGAWVPLR
                     DKGETIGAVVRSKPYAQPVYVSIGHRVSLPLAVRYAMACTTKFRLPETTRWAHRLASG
                     GDSPEISVGAQE"
     gene            4992..5678
                     /locus_tag="AC158Q_02190"
     CDS             4992..5678
                     /locus_tag="AC158Q_02190"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HIR3338606.1"
                     /translation="MSDDPFADQRRQMLAEIVAEAALTAGYTGKDAFSERVMRAIGAT
                     PRHDFVPVELQPYAYLNRPLPIGYEKTVSQPFIVALMTDLLEPDANDVVLEIGAGAGY
                     QAAVLAPLVKRVYTVDIIEELALGAERRLKRLGYGNVDVRVANGYYGWPEHAPYDKII
                     VTAAADLIPPPLLAQLKPGGRMVIPTGIPDKQGLVLAEKSAAGSLSTREILAVRFSEL
                     EDPGAMAGAA"
     gene            5824..>6657
                     /locus_tag="AC158Q_02195"
     CDS             5824..>6657
                     /locus_tag="AC158Q_02195"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR3338607.1"
                     /translation="MARQTRTMAVMFADISESTSLYQKLGDVAARNIVNACLTLISGS
                     LARFDGRLVKTIGDEAMCVFPSADLGVLAASDMQAQVSANRPGNYPVTIHVGLHYGPV
                     LVEDDDVFGDTVNIAAYLAAVAAAEQILTTEATEQSLSAALKSCVRPVFHAVLKGAQE
                     ESTVFQVLWRTDNADLTDVNLHSSKIIPGDTGSLLIALDEERVRVDQWRPSVAIGRSK
                     DCDLVVADSLASRQHLTIKLVRTHFYLFDHSINGTFVSLESGEEVHVLRRELLLDGAG
                     RI"
BASE COUNT         1204 a         2067 c         2228 g         1158 t
ORIGIN      
        1 gaagcaggga tcgttcacga tcggcgacca gaggcgcctg ctcgcgctgt gcgcgcaggc
       61 ggcggtgtcg atcgagaacg cccagctgtt cgaggaagtg agcaattcgc ggggctacaa
      121 cgaaagcatc ctgcgcagca tgagcaatgg cgtgattacg ctcgacgccg aaagcagggt
      181 cagcaagatc aacgagtcag cgcggagaat cctgaaacgg tcggacgacg aaacggtcgg
      241 caggacggtg cacgagatct tcggcgattc caaccgatgg gtgatgaaaa gcctggaccg
      301 ggttatcgga accgggatga tcgacagcac cgtggacgcc aatctccaga tggacgggaa
      361 cgaggtggtt tcggtcaacc tgactgccgt tccgttgcgc gacatgcggg acgaccggat
      421 cggctccatg ctgatcctgg aggacctgac ccgggagaag cggctgcgaa acaccatgtc
      481 ccgctatatg agcaaggcgg tggtggacca gttgctggac aagggcgatg cggtgctggg
      541 cggcaccggc cgggaagtga gcgtgctgtt ctccgacatc cggggcttca ccgccctgtc
      601 ggagcggctg ggggcgcggg agaccgtggc gatgttgaac gagtacttca ccgacatggt
      661 cgatgtcgtg tacgagcaca acggcatcct cgacaagtac atcggcgaca tgatcatggc
      721 ggtattcggg tccgtgctcg cgagcgagga ggacgcggac aacgccgtga ccgttggcag
      781 caggatgctg gtggcgctgc gcgacctgaa cagccggaga ctgaccggag gccacgaacc
      841 gatccgcatc ggcgtcggaa tcagcactgg ctattgcgtg gcgggcaaca tcggctcgcc
      901 gaagcggctc gagtacaccg tcatcggcga ccgggtcaac ctcgcggagc ggctgcagaa
      961 cgcgaacaag tactatggaa ccagcgtgct gatctgccag ccgacggcgg cccggttgaa
     1021 gcgctcggta aagataagag aactcgatct gatacgggtg cggggcatca acgccccggt
     1081 ttccatcttc gaagtgctcg atcaccacac cgcctcgagc tttcccaatc tcgacgtggt
     1141 gctgcctgcc ttcaaggaag ggcttgtgca ttaccgccag agatcctggg accaggcggc
     1201 gaagttcttc agcgaagcgc tctcggcctg tcccggcgat cgaccgtcgc gcatttacct
     1261 ggaacgctgc gaaacctatc ggacggatcc gcctattgcg agctgggatg gcgtctctgt
     1321 ggtccagaat atctagcgtc ctgccgtagc gcagttacat gaacccgggc tgcccgggga
     1381 gggtacgcgt ggcggcggac ggaggcggga tagggaacgc aagaagggcc gggcgccgtg
     1441 ctcgcggcgc ttttttccgg atgtccggcg cgctcaaaag agcgacacgg cctgggcgac
     1501 ccggtccatc aggtaataga acatgaaact ggcggtgacc agcatcgtcc agatcgagcg
     1561 tctggcggcc ccgtgagacc ggcatcgggc ctcgtcgcgg tgcttctcgg tgtgcgcgat
     1621 cgcctggcag taggcataga actggtccac gacccggatg tcgtacttcc tcggcttgtg
     1681 gccggtcccc gccgcggtca tccggctcga cggggaccgc cattgttcaa atgtcgtcat
     1741 gtctgatcca gctgtacggt cctgccgcgg tcaatttccg agggtctgat ccaacccatt
     1801 gattcaatta tgtccaacag ggctcgcccg gacaggggga tggcggcggc agtcgcttgc
     1861 aagtaccgtt ccacggggaa aaagtcacgt tcggcggagg ggccccggtg attattggta
     1921 agggatcggg attgctggca acggctgcag gctgcgccgc actgatcacc agcgtgtggg
     1981 gcgccgacga tcccggcgcg cgggcgctcg agcagcatca gttgcagcgg caacagcagc
     2041 aggaggcgct gcaactgcgc atgcagcagc agcagcgtgc cgtgcagtca actccggcgg
     2101 acccccggca gaagcaggcg caggaaaagc tgcgggcgga ccagcaattg cagcagcagc
     2161 agctgcatta ccggcaagcg atcgagccgg cagccgcgca gccgtcggat gaccaaggcg
     2221 cgcgccgcgc caaggcggag agggaccggc tcgaggcgca gcgtcagggc cagcggcaga
     2281 tacggcaatc cgactgggac ttgcagcagg aagtcggggc ccggaatgag ccgggcgctg
     2341 ccgttccggt gccgccgcgt caattgaaga tacccgctgg cacggaccgg tagccggccc
     2401 gcgcccggcc aatgcggcgc agcgccacta gcgcttcccg acgatctgga acgagtcaaa
     2461 aaacagttcc gcgtcgggcc gcggcttgcc cggcgacacg gccatgacct ggtagaggcg
     2521 gttccccgcc catgccagcc gcaccctgta gtgcgtgcct tctccgctca tgatgatctg
     2581 ttcacggccg gggaagccgg acagcgtgat gtgcttttcg ctcaccatct tgcccttggt
     2641 ctggtacagg agcccgtcgc gcgcgctatc caggagtttc tggggattta ccttggcgac
     2701 ccctgccgcg gggtactcgt tgaacaggac gtcgtaggtc acgttggcgt tatcggtcac
     2761 ggtgtaacgg gtcgcggcga cattgccgat atcggtacgg atggtctgcc gcgatgtctt
     2821 cggcgcaccg ggcatcagca ccttgaaccg cccggcgggt atcgcgactt ccttccagcc
     2881 gggcggggcg ccggcctggg atacggtgcc cgctgcggca agccacaggc acaggccggc
     2941 gagcgcaaaa cagcggaagc gtgcgcggct cattgcggcg gcaggcgcgg cgccgccggc
     3001 cgttcgcgct cgacgaccgt gtagtcggcc gtgatgaatt cttcatccgc cgccttccgc
     3061 agcctgcgtt tcagccacca gaagcgcagc aggacgatgc ccgccagtat cgcccccgcg
     3121 atgagcgcag ccacgagaaa gaagaacccg agcaccagca ctgccagcag gagcacgcca
     3181 tagacgatgc gctccggcag gctgcgggcg cttacgtgga tggcgggcat ggtgagtaac
     3241 ggagcgattc gtgatgggtg attcgggagt cggtcgtggt gtgaaaacaa gccgcaggcc
     3301 ggcagccgtc ctgactcgaa tcacgactca agattcacga cccacgctgt ctcaggaatc
     3361 ggtattgaag ctcttgatga tgaccccgcc ggatttctcg tcgtgctcga gcgtgagcag
     3421 tttgcgcgtt tcggcttcat cgagcagttt tccgaaggag cggaagccgt aataggactc
     3481 gttgaagccg ggttgccggc gcttgagcgc ctgcttcacc atcgagcccc agattttttc
     3541 ctccgccccg cgctctcgca gcagagcctc gaccgtctcc atcaccaggt cgagcgcctc
     3601 ctgtttcttg tcgccgtcgg tggcaggcgc cttgggcgca tcgggggcac cgggcgaagc
     3661 cgccgcgggc ttgcggcggg gagggcgctt ctgtttctcc cgcaccaggt catcgtagta
     3721 gatgaactcg tcgcagttgg cgaccagcag gtcggaggac gattttttca ccccgacgcc
     3781 gatgacggtc ttgttgtttt cgcgcagctt ggagaccagc ggcgagaaat cggagtcgcc
     3841 gctgatgatc acgaacgtgt cgacgtgcga tttggtgtag cagaggtcga gcgcgtccac
     3901 caccatgcgg atatcggccg aattcttgcc ggacatgcgc acgtgcggga tctcgatcat
     3961 ctcgaacgcc gcctcgtgca tcgccttctt gaagtccttg tagcgctccc agtcgcagta
     4021 ggccttcttg acgacgatgc tgcccttgag cagcaggcgc tccaggactt tcttgacgtc
     4081 gaattccgcg tagcgggctt cgcgcacgcc gagcgcgacg ttctcgaaat cgcagaacag
     4141 cgccatgttg ttgctatcgt cctgtgccat cctcgactcc ggttgtgtcc aaccgctaat
     4201 tatccctcat tcctgggccc ccacgctgat ttccggggaa tccccgcccg aggcgagccg
     4261 gtgcgcccag cgcgtcgtct cgggcagacg gaatttcgtg gtgcatgcca tggcgtagcg
     4321 gacggcgagc ggcaggctca cgcggtggcc gatggaaacg tacaccggct gcgcgtaagg
     4381 tttggagcgc accactgcgc ctatggtttc gcctttgtcc ctgagcggca cccacgcgcc
     4441 gcggcgctgc atcggctgac cgtgctcgcc gagcagccgc gacttggcga tcccgatggc
     4501 aggtatgccg caggtcaccc ccatatgaca ggccaggccg aagcggcgcg ggtgcgcgag
     4561 accctggccg tcgcagagga gcaggtcggg ctgtatgcgc aaccggtcga aagccgcgag
     4621 caacgcgggc agctcgcgaa acgacagcag gcccggcacg tacggaaagc gggtgggctg
     4681 gcgcactatc gcgtgatcca ccagttccag ggccgggaag gacagcacgg caactgcggc
     4741 gcgcgtgatg ccgccgccgc gttcaaatcc gacatcggtt cccgcgacat gccgcacctg
     4801 ccggggccgg ccggtcctga tcaccttcga gcgcagccgg tgctggagcg caattgccgc
     4861 ccgcggcgtg aggttccatg tgtggcggat tcttctcatc cctggcgcca gggcaaggct
     4921 tgctgccgat gaacttcaag tacggattgt gtccaacctc ctgtcacggc cactatagta
     4981 gtctataccg catgagcgac gacccgttcg cggaccagcg ccggcagatg ctggcggaga
     5041 tcgtggccga ggccgcgctc accgccgggt ataccggcaa ggacgccttc agcgagcgcg
     5101 tcatgcgcgc catcggtgcc acgccgcgcc acgatttcgt gccggtcgaa ttgcagccct
     5161 atgcctatct caaccgcccg ctgcccatcg gctacgagaa gacggtatcg cagcctttca
     5221 tcgtcgcgct catgaccgac ctgctggaac cggacgcgaa cgacgtggtg ctggagatcg
     5281 gcgccggcgc cggttatcag gcggcggtgc tggccccgct cgtgaagcgc gtgtataccg
     5341 tggacatcat cgaggaactc gcgctggggg cggagcggcg tctgaagcgg ctcggttacg
     5401 ggaacgtcga cgtccgggtc gcgaacggct actacggctg gccggagcac gcgccctacg
     5461 acaagatcat cgtgacggcc gcggcggacc tcatcccgcc gccgctgctg gctcagctca
     5521 agcccggcgg acgcatggtg attcccaccg gcatacccga caagcagggc ctcgtgctgg
     5581 cggagaagtc cgctgccggc agcctgtcca cgcgcgaaat tcttgccgtc aggttttcag
     5641 agctcgagga ccccggcgcg atggcgggcg ccgcctgaat gaggggatga ggggagggaa
     5701 aggagtgtcg aggaatgagt gtcgagtgaa tggaatgtaa cttcgttttt cccctcgaca
     5761 cccgacaccg gccacttgtc ggtcgctcat gttccatgag cgggcgttat aatcggcgcg
     5821 gttatggcgc gtcagacccg gaccatggcg gtgatgttcg ctgacatcag cgagagcacc
     5881 agtctctacc agaaactcgg cgacgtggcg gcgcgcaata tcgtcaatgc ctgcctgacg
     5941 ctgatctccg gcagcctggc gcgcttcgac ggtcggctgg tgaaaacgat cggggacgag
     6001 gcgatgtgcg tcttccccag tgccgacctc ggggttctcg ctgcgagcga catgcaggcg
     6061 caggtttccg ccaaccggcc cggcaattat ccagtgacga tccacgtcgg gctgcactac
     6121 ggtccggtgc tggtggaaga cgacgacgtg ttcggcgaca cggtgaatat tgccgcctac
     6181 ctcgccgcgg tggccgcggc cgaacagatc ctgaccaccg aggccaccga gcaaagcctt
     6241 tcggcggcgc tgaaatcctg cgtgcgcccg gttttccacg ccgtgctgaa gggcgcccag
     6301 gaggagtcga cggtattcca ggtgctgtgg cgcaccgata acgccgatct gaccgacgtc
     6361 aacctgcact cgagcaagat catcccgggc gacaccggca gcctgttgat agcgctcgac
     6421 gaggaaaggg tgcgggtgga ccagtggcgt cccagcgtcg cgatcgggcg ctccaaggac
     6481 tgcgacctgg tggtcgccga ctcgctcgcc tcgcgccagc acctcaccat caagttggtg
     6541 cggacccatt tttatctgtt cgaccacagc atcaacggca ccttcgtctc gctggaaagc
     6601 ggggaggagg tgcacgtgtt gcggcgggaa ttgctgctcg atggtgccgg ccggatc
//
LOCUS       DBNGSG010000086         2178 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2236826, whole genome shotgun sequence.
ACCESSION   DBNGSG010000086 DBNGSG010000000
VERSION     DBNGSG010000086.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2236826"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="AC158Q_02200"
     CDS             complement(<1..183)
                     /locus_tag="AC158Q_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HIR3338608.1"
                     /translation="MKQFRIAVIPGDGIGKEVMPEGLRVMEAAGRQFGIRWQFDEFDW
                     SCDYYKKHGRMMPEDAL"
     gene            complement(295..522)
                     /gene="rpmE"
                     /locus_tag="AC158Q_02205"
     CDS             complement(295..522)
                     /gene="rpmE"
                     /locus_tag="AC158Q_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HIR3338609.1"
                     /translation="MKPKIHPQYSTITVTCSCGNTWDTRSTMGRDLHVEVCSQCHPFY
                     TGKQKIVDTAGRVEKFRQKYARKPATAAATK"
     gene            complement(766..1230)
                     /locus_tag="AC158Q_02210"
     CDS             complement(766..1230)
                     /locus_tag="AC158Q_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338610.1"
                     /translation="MTRAARVLAGCLLAALGGCASVPQASPERDAEAKQFITHPGVST
                     LYVYRGDFPAGMDADDSVLYVNGRLIGSTLPGTYFRIELRPGEHLLHGYAHDQGRIRV
                     RPPAGEITFVSLSVVAGASHFGQVAPETAKREIARCCVLMENWTPGQRPLLR"
     gene            complement(1227..1649)
                     /locus_tag="AC158Q_02215"
     CDS             complement(1227..1649)
                     /locus_tag="AC158Q_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338611.1"
                     /translation="MFLRALLLSILLAGCAQLPPTPQDIEAKKFLSVPDKAVIYLVRT
                     NPDFNSVPAPVWLGESDMITTHPGTYYRWEVEPGRHRIAGFAVDNGTITLQVEAGKIY
                     FVQHSLRPWISYAMSYFQVVSEPQGRAVVMRGELIGAR"
BASE COUNT          369 a          685 c          719 g          405 t
ORIGIN      
        1 gagggcgtct tccggcatca tgcggccgtg cttcttgtag tagtcgcagc tccagtcgaa
       61 ctcgtcgaac tgccaccgga tgccgaactg gcgcccggcc gcttccatga cgcgcaggcc
      121 ctcgggcatc acctccttgc cgatgccgtc gccggggatg acggcgatgc ggaattgctt
      181 catgtcgacc tcgtgaatcg tgaatcgatt ttcccggcgg gcgccggaaa aacaaagggc
      241 agccgcggct gccctttgga tgttacgtga aaatccggcg cggcgccgcg tcccttactt
      301 cgttgcggcg gcggtggcgg gcttgcgcgc gtatttctgg cggaatttct cgacgcggcc
      361 ggcggtgtcc acgatcttct gcttgccggt gtagaacggg tggcactgcg agcacacttc
      421 gacgtgcagg tcgcggccca tcgtggagcg cgtgtcccac gtgttgccgc agctgcaggt
      481 gacggtgatc gtgctgtact ggggatggat cttcggtttc atgcggcctc cggtcattgg
      541 gtgcgctgtc ctccaaccgc tctgtacttt tcccctctcc gctcgggaga gggggaaggg
      601 gtgagggtcg cgcagcgccg atggtttccg agaaagtcgc gcattatcca caatttagct
      661 gcacatggca agccgggccc gaagtaatgg gtgtcgaggg aaagcgttgt aggttcatcg
      721 ctctcgccct tactcactcg acactctttc ctcgacactc ggccctcagc ggagcagcgg
      781 ccgctgtccc ggcgtccagt tctccatcag cacgcagcag cgcgcgatct cgcgcttggc
      841 ggtttcgggg gcgacctgac cgaagtgcga cgcgccggcg accacgctga gcgacacgaa
      901 agtaatctcg ccggccgggg ggcggacccg gatccggccc tggtcgtggg catagccgtg
      961 cagtaggtgc tcgcccgggc gcagttcgat gcggaagtag gtgccgggca gcgtggagcc
     1021 gatcaggcga ccgttcacgt agagcacgct gtcgtccgca tccatgccgg cgggaaagtc
     1081 gccgcggtag acgtacaagg tggaaacacc aggatgcgtg atgaattgct tcgcctccgc
     1141 atcgcgctcc ggcgacgcct ggggcacgga cgcgcagccg ccgagcgcgg cgagcaggca
     1201 gccggcgagt acccgcgctg cgcgcgtcac cgggcgccga tcaattcgcc gcgcatgacc
     1261 accgcgcggc cctgcggttc ggaaaccacc tggaaatacg acatcgcgta actgatccac
     1321 ggtctcaggc tgtgctggac gaagtaaatc ttgccggcct ccacctgcaa cgtgatggtt
     1381 ccgttatcca cggcgaaccc cgcgatgcga tgccggcccg gctcgacttc ccagcggtag
     1441 taggtcccgg gatgggtcgt gatcatgtcg ctctcgccga gccagaccgg cgccggcacg
     1501 ctgttgaaat ccgggttggt gcgcacgaga tagatcaccg ccttgtccgg cacgctcagg
     1561 aatttcttgg cctcgatatc ctgcggcgtg ggcggcagct gcgcgcagcc cgcgaggagt
     1621 attgataaca acagtgctct caggaacatg ggaccctcca tgagtccacg aaggcgccct
     1681 tagtatagcc ccgctcccgc agccaaagct gacaggttcg taagcaattg ttaccggaat
     1741 aagcgcgcgc tgtcgtgtgt ggggatgcga cccgggagac ggccgcgatt tcgggccgga
     1801 agcaggagag ctgaaacgtc cgccggggat gctaagcgcg attacccccg acgcatggag
     1861 tcaaaaaagt ccgaattgct cttcgtcgcc ctgaccttgt ccaggaggaa ctcggtggct
     1921 tccagcccgt ccggagggtc gcgccgcgac tcgttagcgg aacgcccgtt tgcgggagcg
     1981 gcgcggttac ccgatggctg actacccccg acgcatggaa tcaaaaagtc cgaattgctc
     2041 ttcgtcgccc tgaccttgtc caggaggaat tcggtggctt ccagcccgtc cggagggtcg
     2101 cgccgcgact cgttagcgga acgcccgttt gcgggagcgg cgcggttacc cgatggctga
     2161 ctacccccga cgcatgga
//
LOCUS       DBNGSG010000087         2388 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2576327, whole genome shotgun sequence.
ACCESSION   DBNGSG010000087 DBNGSG010000000
VERSION     DBNGSG010000087.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2576327"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="AC158Q_02220"
     CDS             <1..226
                     /locus_tag="AC158Q_02220"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR3338612.1"
                     /translation="PQYGNMIEIDHGNDLVSRYAHASKRLVKIGDVVLRGAKIAEVGM
                     TGRSTGPHLHFEVRQRGAPANPSQFLRLPG"
     gene            289..>2388
                     /gene="secA"
                     /locus_tag="AC158Q_02225"
     CDS             289..>2388
                     /gene="secA"
                     /locus_tag="AC158Q_02225"
                     /inference="COORDINATES: protein motif:HMM:NF009538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIR3338613.1"
                     /translation="MIQSILKKIFGSRNDRLLKQYQRQVRAINALEPQIQKLSDGALR
                     LKTVEFRKRIEERLAKIPDGAGADGARAAARRQGLEELLPEAFAVVREAGRRTLKMRH
                     FDVQLLGGIALHNGKIAEMRTGEGKTLVATLPAYLNSLTGLGVHIVTVNDYLAQRDAE
                     WMGKIYRFLGLTVGVNLSQMEHDLKQQAFAADITYGTNNEFGFDYLRDNMVYQTSERV
                     QRGLNYAIVDEVDSILIDEARTPLIISGQAEDTTELYVRINQLAPKLARQKDEKAPGD
                     YWVDEKAHQVILSEQGHEHAEQLLEGAGLLTPNSSLYDPANIILMHHLNAALKAHNLF
                     LRDQQYVVQGNEIIIVDEFTGRLMPGRRWSEGLHQAVEAKEGVEIQKENQTLATITFQ
                     NYFRMYRKLAGMTGTADTEAYEFQQIYGLETVLIPTHMAMIREDRMDQVYRTANEKHQ
                     AIINDIKDCYGRGQPTLVGTTSIENSEMLSEMLKKENLPHNVLNAKQHAREAEIVIQA
                     GRPKMVTIATNMAGRGTDIVLGGNPEPEINLVEADATLDEAAKKQRIEEIRFNWQKLH
                     NQVVGAGGLHIVGTERHESRRIDNQLRGRSGRQGDPGSSRFYLSLEDPLLRIFASDRV
                     AAIMERLKMPEGEAIEHRWVTRSIENAQRKVEARNFDIRKHLLEYDDVANDQRKVVYE
                     QRNRLMDVDDISESVANI"
BASE COUNT          513 a          729 c          763 g          383 t
ORIGIN      
        1 ccctcagtat ggtaatatga tcgagatcga tcacggcaat gatctggtct cgcggtatgc
       61 gcacgcatcg aagcgcctgg tgaagattgg ggacgtggtg ttgcgcggcg ccaagatcgc
      121 ggaggtcggc atgaccgggc gttcaaccgg cccgcatctg cacttcgagg tgcggcagcg
      181 tggagcaccg gcaaacccgt cgcagttcct gcgattgccc ggttgatttg acagacagga
      241 atttcgaagg gggggtgggg cgtttcccca cccctttttg tttagctgat gattcaatcc
      301 atactcaaga agatcttcgg cagccgcaat gaccggctgc tcaagcagta tcagcggcag
      361 gtacgcgcga tcaacgcgct cgagccgcag atccagaaac tcagcgacgg cgcgctgcgc
      421 ctgaagacgg tcgagttccg caagcgtatc gaagagcggc tcgcgaagat tccggacggc
      481 gccggcgccg atggcgcgcg cgcggccgcg cggcgccagg ggctcgagga attgctgccg
      541 gaggcgtttg cggttgtgcg cgaggcgggg cggcgcacgc tcaagatgcg ccatttcgac
      601 gtgcagctgt tgggcggcat cgcgctccat aacggcaaga tcgccgagat gcgcaccggg
      661 gaaggcaaga cgctggtggc tacccttccc gcgtatctca actccctcac cggcctgggt
      721 gtgcatatcg tgaccgtcaa cgattacctg gcgcagcgtg atgccgagtg gatgggcaag
      781 atttaccgct tcctggggct cacggtcggc gtcaacctgt cgcagatgga gcacgacctc
      841 aagcaacagg cctttgccgc ggacatcact tacggcacca acaacgagtt cggcttcgac
      901 tacctgcgcg acaacatggt ctatcagacc tcggaacggg tgcagcgcgg gctcaactac
      961 gcgatcgtgg acgaggtgga ctcgatcctg atcgacgagg cgcgcacgcc gctcatcatt
     1021 tccgggcagg ccgaggacac caccgagctc tatgtcagga tcaaccagct tgcgcccaag
     1081 ctcgcgcgcc agaaagacga gaaggcgccc ggtgattact gggtggacga gaaggcgcac
     1141 caggtgatcc tgtccgaaca gggccacgag catgccgagc agttgctcga gggggccggc
     1201 ctgctcacgc ccaactcgag cctctacgat ccggccaaca tcatactcat gcaccacctc
     1261 aacgcggcgt tgaaagcgca caacctgttc ctccgcgacc agcagtacgt ggtgcagggc
     1321 aacgagatca tcatcgtgga cgagttcacc gggcggctga tgcccgggcg gcgctggtcg
     1381 gaaggcctgc accaggcggt cgaggcgaag gaaggcgtgg agatccagaa ggaaaaccag
     1441 accctcgcaa cgattacctt ccagaattac ttccgcatgt atcgcaagct ggccggcatg
     1501 accggcacgg cggacaccga ggcctacgag ttccagcaga tctacgggct ggagaccgtg
     1561 ctgattccca cccacatggc gatgattcgc gaagaccgca tggaccaggt ctatcgcacg
     1621 gcgaacgaga agcaccaggc gatcatcaac gacatcaagg attgctacgg gcgcggccag
     1681 ccgacgctgg tcggcaccac ctcgatcgag aattccgaga tgctctcgga gatgctcaag
     1741 aaggagaatc tgccgcacaa cgtgctcaat gccaagcagc acgcgcgcga ggcggaaatc
     1801 gtgatccagg ccgggcgtcc caagatggtc actatcgcca ccaatatggc gggacgcgga
     1861 accgacatcg tgctcggcgg caatcccgag cccgaaatca acctggtcga agccgacgca
     1921 acgcttgatg aagcggcgaa gaagcagaga atcgaggaga tccgcttcaa ctggcagaaa
     1981 ttgcacaacc aggtggtcgg ggccggcggt ctgcacatcg tcggcaccga gcgccacgag
     2041 tcgcgccgca tcgacaacca gttgcgcggt cgttccggtc gccagggcga cccgggatcg
     2101 agccggttct acctctcgct cgaggacccg ctgctgcgca tcttcgcctc ggaccgggtg
     2161 gccgcgatca tggagcggct gaagatgccg gaaggcgagg cgatcgagca ccgctgggtg
     2221 acgcgctcga tcgagaatgc ccagcgcaag gtcgaggcgc gcaatttcga catccgcaag
     2281 cacctgctcg aatacgacga cgtcgccaac gaccagcgca aggtggtgta cgaacagcgc
     2341 aaccggttga tggatgtcga cgatatttca gagagcgtcg ccaacatc
//
LOCUS       DBNGSG010000088         3376 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3086137, whole genome shotgun sequence.
ACCESSION   DBNGSG010000088 DBNGSG010000000
VERSION     DBNGSG010000088.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3086137"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="AC158Q_02230"
     CDS             <1..468
                     /locus_tag="AC158Q_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HIR3338614.1"
                     /translation="QRREWAHEGELEWRVTELPEHAGTQRWVADLNRFYRNTPALWQL
                     DFSNEGFEWIDANDAGQSVLTFLRKPGGAGGQVLVACNLTPVPRENYFIGVPRAGVWT
                     ETLNSDARVYGGSGWGNLGKVEAAPVGAHGRPYSIVVTLPPLSTIYFESAADG"
     gene            461..2470
                     /locus_tag="AC158Q_02235"
     CDS             461..2470
                     /locus_tag="AC158Q_02235"
                     /EC_number="2.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009313 - oligosaccharide catabolic process
                     [Evidence IEA];
                     GO_process: GO:0030979 - alpha-glucan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HIR3338615.1"
                     /translation="MAETSLPPAAPEGRARAVIDAVLPAVDGGRFAVKRIVGDRLDVE
                     AHCFADGHDALRVLLQWREEEAADWNEVEMRALGNDVWRASFVPGQIGRYRYTVVAWV
                     DHFLTWRTEFAKREDLADLQLAAWVGAGLVEEAARRAARDDAKRLEDWAALLRAGGDI
                     EVIRAVALDEAMADIALRYPDRGWATAWRAAMPLVVDRDRARYSTWYELFPRSTSDTP
                     GRHGTLKDVEKRLPYVAELGFDVLYLPPIHPIGREARKGRNNALEAGPDDVGSPWAIG
                     AAEGGHKAIHSQLGTIGDFRSLVAAAKRHGMEIALDIAYQCAPDHPYVKAHPTWFRWR
                     PDGSVQYAENPPKKYQDIYSFNFESEDWRGLWAELKSVFDFWIGEGVRIFRVDNPHTK
                     PFAFWEWAIAEIRRDHPDVIFLAEAFTRPKVMHRLAKLGYTQSYTYFAWRNSKRELTE
                     YFAELSQGAGREYFRPNVWPNTPDILTDALQFGGRPAFIMRLVLAATLSANYGIYGPA
                     YELMEHLARDLGSEEYLDSEKYQLRHWNLARADSLRPLVSAVNRIRRENPALQSDWRL
                     RFFDVDNDQMIAYGKTTEDLGNAIVCVVSLDPHNVHSGWVTLDLDALGIDPGAQYQMH
                     DLLTGARYRWSGPRNFVRIDPQRVPAHVFHVHRRAHREQDFDYFL"
     gene            2487..>3376
                     /locus_tag="AC158Q_02240"
     CDS             2487..>3376
                     /locus_tag="AC158Q_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIR3338616.1"
                     /translation="MDAPITGSPQVVSAAAVDDPLWYKDAVIYQLHVKAFLDSNGDGL
                     GDFRGLTEKLDYVQELGVNTIWLLPFNPSPLKDDGYDVSDYHDVHPNYGTRADFRHFV
                     REAHRRGLRVITELVVNHSSDQHPWFQAARRAPAGSSKRNFYVWSDTDQKYKGTRIIF
                     TDTETSNWTWDPVAKAYYWHRFFSHQPDLNFDNPNVLRAVVRTMRHWLDMGVDGFRLD
                     AIPYLVEREGTSNENLAETHAVIKKIRAALDARYTGKLLLAEANQWPEDVREYFGESD
                     ECHMAYHFPLMPRMYMAIAQ"
BASE COUNT          598 a         1059 c         1148 g          571 t
ORIGIN      
        1 cagcggcgcg aatgggcgca cgagggcgag ctcgagtggc gggtcactga actgccggag
       61 cacgccggca cgcaacgctg ggtcgccgac ttgaatcgct tctaccgaaa cactccggcg
      121 ctgtggcagt tggatttctc gaacgaagga ttcgagtgga tcgacgcgaa cgacgccggg
      181 cagagcgtgc tgaccttcct gcgcaagccg ggcggcgcgg gcgggcaggt gctggttgcc
      241 tgcaatctga cgccggtgcc gcgcgaaaac tatttcatcg gcgtgccgcg cgcgggcgtc
      301 tggacggaaa cgctcaacag cgatgcccgc gtatacggag gctcgggatg gggcaacctc
      361 gggaaagtcg aagcggcgcc cgtcggggcg cacggccgcc cgtattcgat cgtcgtgacg
      421 ctgccgcccc tgtccaccat ttatttcgag agtgccgccg atggctgaaa cgagtcttcc
      481 gccggcggcg cccgaaggcc gcgcgcgggc ggtgatcgac gccgtgctgc ccgcggttga
      541 tggcgggcgc ttcgccgtca agcgcatcgt gggcgaccgc ctggacgtgg aggcgcactg
      601 cttcgcggac ggccacgacg cgctgcgtgt gctgttgcag tggcgggaag aagaagccgc
      661 ggactggaac gaggtggaaa tgcgggcact gggcaacgat gtgtggcgcg caagctttgt
      721 tcccgggcag atcgggcgct atcgctatac ggtcgtcgca tgggtggatc atttcctgac
      781 ctggcgcacc gagtttgcca agcgcgagga cctcgccgac ctgcagctgg cggcctgggt
      841 cggggcgggc ctggtcgaag aggcggcgcg acgcgcggcc cgtgacgacg cgaagcggct
      901 cgaggactgg gccgcgctgc tgcgcgcagg gggcgacatc gaggtgatac gcgcggtcgc
      961 gctcgatgaa gcgatggcgg atatcgctct gcggtatccg gaccgggggt gggcgaccgc
     1021 ctggcgggcg gccatgcctc tcgtcgtcga ccgcgaccgg gcgcgctatt cgacatggta
     1081 cgagctgttt ccgcgctcga catcggatac gcccggacgc catggcacgc tgaaggacgt
     1141 ggagaagcgt ttgccgtacg tggccgaact ggggttcgac gtgctgtacc tgccgccgat
     1201 tcatccaatc gggcgcgaag cgcgcaaggg ccgcaacaac gcgctcgagg ccggaccgga
     1261 cgatgtcggc agtccctggg caatcggtgc ggctgagggc ggacacaagg ccatccattc
     1321 gcagttgggg accatcgggg attttcgcag cctggtcgcg gccgcgaagc gccacggcat
     1381 ggagatcgcc cttgatatcg cctatcagtg cgcgcccgac catccctacg tgaaggcgca
     1441 tccgacgtgg ttccgctggc gcccggatgg cagcgtgcaa tacgcggaga atccgcccaa
     1501 gaagtaccag gacatctact cgttcaactt cgagtccgag gactggcgcg gcctgtgggc
     1561 ggaactgaaa agcgtgttcg atttctggat cggggagggc gtgcgcatat tccgcgtcga
     1621 caacccacac accaagccgt tcgctttctg ggagtgggcg atcgcggaga tccggcgaga
     1681 ccatcccgat gtgatcttcc tcgccgaggc cttcacgcgg ccgaaggtga tgcatcgcct
     1741 ggcaaagctc ggctacacgc agtcctacac ttatttcgcg tggcgcaatt ccaagcgcga
     1801 gttgacggag tatttcgccg agctgagcca gggggcgggc cgcgaatatt tccgtccgaa
     1861 tgtgtggccc aatacacccg acatcctgac cgacgctttg cagttcggcg gccggccggc
     1921 gttcataatg cggctggtgc tggcggcgac gctttcggcg aactacggca tctacgggcc
     1981 ggcctatgaa ctgatggagc atttggcgcg cgatctcggc agcgaggagt acctcgactc
     2041 cgagaagtac cagttgcgcc actggaacct cgcgcgcgcc gacagcctgc gtccgctggt
     2101 gagcgcggtg aatcgcatcc ggcgcgagaa ccccgcgctg cagtcggact ggcgcttgcg
     2161 ctttttcgac gtcgacaacg accagatgat cgcctacggc aagacgacgg aagacctcgg
     2221 caacgcaatc gtgtgcgtcg tgagcctcga cccgcacaac gtgcactcgg gctgggtcac
     2281 gctcgacctc gatgccctcg gtatcgatcc cggggcgcag tatcagatgc acgatctgct
     2341 gactggcgcg cgttatcgct ggtcggggcc gcggaacttt gtccggatcg atccgcagcg
     2401 cgtccccgcg cacgtcttcc acgtgcaccg gcgcgcgcat cgcgagcagg acttcgatta
     2461 tttcctgtga cggtgaaccg aacgccatgg acgcgcccat aacgggaagc ccgcaggtcg
     2521 tttccgccgc cgcggtggat gatcccctgt ggtacaagga cgcggtcatc tatcagctgc
     2581 acgtcaaggc cttcctcgat tcgaacggcg acgggctggg cgatttccgc gggctcaccg
     2641 agaagctcga ttacgtccag gaactgggcg tgaacacgat ctggctgctg ccgttcaatc
     2701 cctcgccgct caaggatgac ggctacgacg tctcggatta ccacgatgtt caccccaatt
     2761 acggcacccg ggcggacttc cgccacttcg tgcgcgaggc gcaccggcgg gggctgcgcg
     2821 tgatcaccga gttggtggtc aatcacagtt ccgaccagca tccgtggttc caggccgcgc
     2881 gccgggcgcc ggcgggatcg tcgaagcgca acttctacgt gtggagcgac accgaccaga
     2941 aatacaaggg cacccgcatc atcttcaccg ataccgagac ctcgaactgg acctgggacc
     3001 cggtggcgaa agcgtattac tggcatcgct tcttctcgca ccagccggac ctcaacttcg
     3061 acaatccgaa cgtgctcagg gcggtggtac gcacgatgcg ccactggctc gacatgggcg
     3121 tggacggatt ccggctggac gcgattccct acctggtgga gcgcgagggc acgagcaacg
     3181 agaacctggc cgagacccac gcggtgatca agaaaatccg ggcggcgctc gatgcgcgct
     3241 acaccggcaa gctgcttctc gcggaggcca accagtggcc cgaggacgtg cgcgagtact
     3301 tcggcgagag cgacgaatgc cacatggcgt atcacttccc gctgatgccg cgcatgtaca
     3361 tggcgatcgc gcagga
//
LOCUS       DBNGSG010000089         3438 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1897119, whole genome shotgun sequence.
ACCESSION   DBNGSG010000089 DBNGSG010000000
VERSION     DBNGSG010000089.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1897119"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /gene="rpoA"
                     /locus_tag="AC158Q_02245"
     CDS             complement(<1..646)
                     /gene="rpoA"
                     /locus_tag="AC158Q_02245"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HIR3338617.1"
                     /translation="MASSTFLKPRIIDVQNLSPYHARLTMEPFERGYGHTLGNALRRT
                     LLSSLPGYAATEVKIGGVLHEYSTLDGVQEDVVDLLLNLKGIVLKMHNREEALLSLKK
                     SGEGVVTAGDIEPMHDVEIVNPDHVIAHLAAGGKLDMEIKVVQGRGYIAATTRKTNPE
                     EGRTVGSILLDASFSPVRRVSYAVEAARVEQRTDLDKLVIDVETNGAIDPEESVR"
     gene            complement(688..1314)
                     /gene="rpsD"
                     /locus_tag="AC158Q_02250"
     CDS             complement(688..1314)
                     /gene="rpsD"
                     /locus_tag="AC158Q_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HIR3338618.1"
                     /translation="MARNLDAKCRQCRREGEKLFLKGEKCFTDKCAIERRNYPPGQHG
                     QKNTRLSGYGVQLREKQKVRRIYGLLERQFRKIYHDAEQKKGITGENLLQLLESRLDT
                     VAYRMGFGSSRTEARQIVRHNGILVNGRRVNIPSFVCLPGDVIEVAAKAKQQLRVKGA
                     AEAAEGRGFPEWLEVDAKALKGRFKARPQRTELPSTINESLVVELYSK"
     gene            complement(1392..1790)
                     /gene="rpsK"
                     /locus_tag="AC158Q_02255"
     CDS             complement(1392..1790)
                     /gene="rpsK"
                     /locus_tag="AC158Q_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HIR3338619.1"
                     /translation="MAKASTRVRKKVKKNVAEGIAHIHASFNNTIVTITDRQGNTLAW
                     ATCGSNGFKGSRKSTPFAAQVAAGRAGALAQECGVKNLEVHIKGPGPGRESAVRALNA
                     AGFKITQISDVTPVPHNGCRPPKKRRVEDR"
     gene            complement(1804..2181)
                     /gene="rpsM"
                     /locus_tag="AC158Q_02260"
     CDS             complement(1804..2181)
                     /gene="rpsM"
                     /locus_tag="AC158Q_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HIR3338620.1"
                     /translation="MARIGGVNIPNHQHAEIALTAIFGIGRARARMICAAAGVSHAAK
                     VKDLSDSQMDKLREQVSKVTVEGELRREVSMNIKRLIDLGTWRGKRHRLGLPVRGQRT
                     RTNARTRKGPRKAAVKIIRAVTA"
     gene            complement(2228..2341)
                     /gene="rpmJ"
                     /locus_tag="AC158Q_02265"
     CDS             complement(2228..2341)
                     /gene="rpmJ"
                     /locus_tag="AC158Q_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HIR3338621.1"
                     /translation="MKVLASVKRICRNCKIVRRKRVVRVICKNPRHKQRQG"
     gene            complement(2381..2599)
                     /gene="infA"
                     /locus_tag="AC158Q_02270"
     CDS             complement(2381..2599)
                     /gene="infA"
                     /locus_tag="AC158Q_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018149800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HIR3338622.1"
                     /translation="MAKEETIQMQGEIVETLPNATFRVKLENGHVVLGHISGKMRMHY
                     IRILPGDKVTVELTPYDLSRGRIVFRAR"
     gene            complement(2599..>3438)
                     /gene="secY"
                     /locus_tag="AC158Q_02275"
     CDS             complement(2599..>3438)
                     /gene="secY"
                     /locus_tag="AC158Q_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HIR3338623.1"
                     /translation="TMFLMWLGEQITERGIGNGISLIIFAGIAAGFPRAVGGTLELAR
                     TGAFSIPLVLLLLVGAVLVTGLVVFVERGQRKILVNYAKRQVGRKVYGGQSSHLPLKL
                     NMAGVIPPIFASSIILFPATLAGWFGSNEGMNWLRDISGKLSPGQPIYVLLYASAIVF
                     FCFFYTALVFNPKETAENLKKGGAFVPGIRPGDQTAKYIEKIIMRLTLVGAIYVTLVC
                     LLPEFLIVKWNVPFYFGGTSLLIIVVVTMDFMAQVQAYVMSHQYESLLKKANFKGGMF
                     PTR"
BASE COUNT          535 a         1136 c         1066 g          701 t
ORIGIN      
        1 agcgcacgga ctcctcgggg tcgatcgccc cgttggtctc gacgtcgatc accagcttgt
       61 cgaggtcggt gcgctgctcg acgcgcgccg cctcgaccgc gtagctcacg cggcgcaccg
      121 ggctgaacga cgcgtcgagc aggatgctgc cgaccgtacg cccttcctcg ggattggtct
      181 tgcgcgtcgt cgcggcgata tagccgcggc cctgcacgac cttgatctcc atgtcgagct
      241 tgccgccggc ggcgaggtgc gcgatgacgt ggtcgggatt gacgatctcg acgtcgtgca
      301 tcggctcgat gtcgccggcg gtgacgaccc cttcgcccga tttcttgagc gagagcagcg
      361 cctcctcgcg gttgtgcatc ttcagcacga tgcccttcag gttcagaagc aggtcgacca
      421 cgtcctcctg cacgccgtcg agcgtggagt actcgtgcag cacgccgccg atcttgacct
      481 cggtcgcggc atagcccggg agcgaggaga gcagcgtgcg ccggagcgcg ttgcccagcg
      541 tgtgcccgta gccgcgctcg aacggctcca tcgtgagccg ggcgtggtag ggcgagaggt
      601 tctggacatc gatgatgcgg ggcttgagaa aggtgctgct tgccatggtt tggtgacctc
      661 tgcggtttac ttcggttcct gtgcgaatta cttcgaatag agctcgacca ccagcgactc
      721 gttgatggtc gacggaagct ccgtgcgttg cggccgcgcc ttgaatctgc ccttgagcgc
      781 cttggcgtcg acttccagcc attccggaaa gccgcgcccc tccgcggctt ccgccgcgcc
      841 cttcacgcgc agctgctgct tggccttggc cgcgacctcg atcacgtcgc cgggcaggca
      901 gacgaaggac ggaatgttga cgcgccggcc gttcaccagg atgccgttgt ggcgcacgat
      961 ctggcgcgcc tcggtgcggg aggagccgaa acccatgcgg taggcgacgg tgtcgagccg
     1021 gctctccagc agctgcagca ggttctcgcc ggtgatgccc ttcttctgct cggcatcgtg
     1081 atagatcttg cggaattgcc gctcgagcag cccgtagatg cgccgcacct tctgcttctc
     1141 gcgcagctgc acgccgtagc cggagaggcg ggtgttcttc tggccgtgct ggcccggcgg
     1201 ataattgcgg cgctcgatgg cgcacttgtc ggtgaagcat ttctcgccct tgaggaagag
     1261 cttctccccc tcccggcggc actgccggca cttcgcgtcg agattcctgg ccatgttcgt
     1321 ttactacctc ttcatcctat gtaggggtgc acgaggggag tatcccctcg ttcccggctc
     1381 ttacctcttc gtcacctgtc ctcgacgcgc cgcttcttcg gcgggcggca gccgttgtgc
     1441 ggcaccggcg tcacgtcgga tatctgggtg atcttgaagc cggccgcatt gagcgcgcgc
     1501 accgccgact cgcgccccgg tcccgggccc ttgatgtgca cttcgaggtt cttcacgccg
     1561 cactcctggg cgagcgcgcc cgcgcgtccg gccgccacct gtgcggcgaa cggcgtgctc
     1621 ttgcgcgagc ccttgaagcc gttcgagccg cacgtcgccc atgcgagcgt gttgccctgg
     1681 cggtcggtga tggtgacgat ggtgttgttg aaggacgcgt ggatgtgggc gatgccctcc
     1741 gccacgttct tcttgacctt cttgcgtacc cgggtgctgg ctttggccat gtctggatcc
     1801 tcgttaagcg gtgaccgcgc ggatgatctt gaccgcggct ttgcgcgggc ccttgcgcgt
     1861 gcgcgcgttg gtgcgggtgc gctggccgcg caccggcagc cccaggcggt ggcgcttgcc
     1921 gcgccaggtg ccgagatcga tcaggcgctt gatgttcatc gagacttcgc gccgcagctc
     1981 gccctcgacg gtgaccttgg acacctgctc gcgcagcttg tccatctggc tgtccgagag
     2041 atccttgacc ttggcggcat ggctgacgcc ggcggcggcg cagatcatgc gcgcgcgcgc
     2101 gcggccaatg ccgaagatcg cggtcagcgc gatctccgcg tgctgatggt tgggaatatt
     2161 cactccgccg attcgtgcca tgcttctatc ctctcgtcac tcgtcactga tcactcatca
     2221 ctgtcaatca gccctggcgc tgcttgtggc gcgggttctt gcagatcacc cgcactacgc
     2281 gcttgcggcg gacgatcttg caattgcgac aaattctctt caccgatgcc agcactttca
     2341 tctctgtgtc ctcaaatcac gatccgcgaa tcacgcgccg ttaccgggcg cggaacacga
     2401 tgcgcccgcg cgacaggtcg tagggcgtca gctctaccgt caccttgtcc cccggcagga
     2461 tgcggatata gtgcatgcgc atcttgcccg agatgtggcc gagcaccacg tgcccgttct
     2521 ccagcttcac ccgaaatgtc gcgttgggca gggtctccac gatctcgccc tgcatctgta
     2581 tggtctcttc cttggccatc agcgcgtcgg aaacatgcca cccttgaagt tggccttctt
     2641 gagcaggctc tcgtactgat gcgacatgac gtatgcctgc acctgcgcca tgaagtccat
     2701 cgtgaccacg acgatgatca gcaagctggt gccgccgaag tagaacggca cgttccactt
     2761 cacgatcagg aactccggca gcaggcacac cagcgtcacg tagatcgcgc ccactagcgt
     2821 cagccgcatg atgatcttct cgatgtattt cgccgtctgg tcgcccgggc ggattcccgg
     2881 cacgaacgct ccgcctttct tcaggttctc cgccgtttcc ttggggttga acaccagggc
     2941 cgtataaaag aaacagaaga acacgatcgc agacgcgtac aacaacacgt aaatcggctg
     3001 tcccggggac agtttgccgc tgatgtcccg caaccagttc atgccttcgt tgctcccgaa
     3061 ccagccggcc agcgtggccg gaaacaggat aatgctcgag gcaaatatcg gcgggatcac
     3121 tccggccatg ttcaatttca gcggcaggtg cgaactctgc ccgccataga ccttgcggcc
     3181 gacctggcgc ttggcgtagt tgaccaggat tttgcgctgg ccgcgttcga cgaacaccac
     3241 cagcccggtc accagcaccg cacccaccag cagcagcagc accagcggga tcgagaacgc
     3301 gccggtgcgc gccagttcca gcgtgccgcc gactgcacgc ggaaacccgg cggcaatgcc
     3361 cgcgaaaatg atcagcgaaa tgccgttgcc gattccgcgc tcggtgatct gttcgccgag
     3421 ccacatcagg aacatggt
//
LOCUS       DBNGSG010000090         5109 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1897438, whole genome shotgun sequence.
ACCESSION   DBNGSG010000090 DBNGSG010000000
VERSION     DBNGSG010000090.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5109)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5109)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5109
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1897438"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /gene="hisH"
                     /locus_tag="AC158Q_02280"
     CDS             <1..522
                     /gene="hisH"
                     /locus_tag="AC158Q_02280"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HIR3338624.1"
                     /translation="DCVREMDERGLRDAVLEAARVKPFLGICIGLQMLFDRSEEGDTP
                     GLGLLPGRVRRFPAQGMVDAQGARLKVPHMGWNEVRQTAAHALWDGIPQGERFYFVHS
                     YFPEPASRGVTAGEAVYGVPFACAAARDNVFAVQFHPEKSNTAGLRLLANFLAWQPAA
                     PNPRQRTAATPAA"
     gene            537..1289
                     /gene="hisA"
                     /locus_tag="AC158Q_02285"
     CDS             537..1289
                     /gene="hisA"
                     /locus_tag="AC158Q_02285"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HIR3338625.1"
                     /translation="MLIIPAIDLKDGKCVRLKQGAMDKATVFSDDPVAMARQWLKQGA
                     RRLHVIDLNGAAAGRPKNEPAIRAIAAAVGDKLPIQLGGGIRDLDTIEQLIDSGVGYV
                     IIGTAAVKTPGFLHEACTAFPGRVLVALDAKDGKVAVDGWSKMTGHEVLDLAKKFQDY
                     GVEAIIYTDIGRDGMLTGVNIEATVTLARALSVPVIASGGINGLDDVKALCKVEHEGI
                     TGAITGRAIYEGKLDFEKAQKLADQISKKGES"
     gene            1356..2126
                     /gene="hisF"
                     /locus_tag="AC158Q_02290"
     CDS             1356..2126
                     /gene="hisF"
                     /locus_tag="AC158Q_02290"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012362933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HIR3338626.1"
                     /translation="MGLAKRIIPCLDVTAGRVVKGINFVGLRDAGDPVEVARRYNEQG
                     ADELVFLDITASSDGRDLIVSVVEAVAAQVFIPLTVGGGVRAVEDVRRLLLAGADKVS
                     INTAAVQNPRLVKDATARFGSQCIVVAVDARRVPGEGAPRWEVYTHGGRNPTGLDAVE
                     WGRTMEAAGAGEILLTSMDRDGTRNGFDLELTRAFSDALSIPLIASGGVGNLEHLAEG
                     IIQGRADAVLAASIFHYGEYTVRQAKEHMARRGIEVRL"
     gene            2223..2615
                     /gene="hisI"
                     /locus_tag="AC158Q_02295"
     CDS             2223..2615
                     /gene="hisI"
                     /locus_tag="AC158Q_02295"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HIR3338627.1"
                     /translation="MKDDWLDQVKWAQDGLVPAVTQESGSGRVLTVAWMNREALAKTV
                     ETGEAHYWSRSRKKLWRKGEESGHVQQVRAIRLDCDEDVVLLEVEQAGGIACHTGRHS
                     CFYRKLDGGKWIITDPVLKDPGGIYKKA"
     gene            2652..2972
                     /locus_tag="AC158Q_02300"
     CDS             2652..2972
                     /locus_tag="AC158Q_02300"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HIR3338628.1"
                     /translation="MTNAEILKRLGEVLAARRQADPSSSYVASLFAKGEDAILKKLAE
                     EAAETVLAAKGGDKLQIVRETADLWFHSLVLLAWHGLKPEDVLAELRRREGISGIDEK
                     KSRK"
     gene            2997..3227
                     /gene="tatA"
                     /locus_tag="AC158Q_02305"
     CDS             2997..3227
                     /gene="tatA"
                     /locus_tag="AC158Q_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HIR3338629.1"
                     /translation="MGSFSIWHWLIVLLVVILIFGTKKLSNLGKDLGSAVKGFKDGMK
                     PEDDKSAQAKTEIPPTTGQTIEGEAKKETPKA"
     gene            3309..3722
                     /gene="tatB"
                     /locus_tag="AC158Q_02310"
     CDS             3309..3722
                     /gene="tatB"
                     /locus_tag="AC158Q_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - transmembrane protein
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HIR3338630.1"
                     /translation="MFDIGFSELLLIAVVALIVIGPERLPKVARTLGHLFGRMQRYVN
                     DVKADISREMELEELKKMQSSMEETARSMRDSVERGISETEGELNKVAQSAQPKIDPY
                     DDPFADNPPPEAAPASPQGELPLEPGQQPPGQKQA"
     gene            3816..4589
                     /gene="tatC"
                     /locus_tag="AC158Q_02315"
     CDS             3816..4589
                     /gene="tatC"
                     /locus_tag="AC158Q_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HIR3338631.1"
                     /translation="MTEDTFISHLIELRDRLLRALIAVGVIFLILFPFASELYDLLAY
                     PLMRALPEGSKMIATGVITPFLIPVKIAVLMAFAVALPYVLYQAWAFIAPGLYAHERR
                     LVIPLVVTSTLLFFVGVAFCYFIVFGVVFDFIYQIAPKSISVAPDIENYLNFAITLFL
                     AFGATFEVPIVVLILVRMGMVTIEQLRSIRPYFIVGAFILAAIITPPDVISQLLLAIP
                     MCLLYEVGIIAAMLFGRAPPKAAEDGAGDGMGQEPGAKP"
     gene            complement(4615..>5109)
                     /locus_tag="AC158Q_02320"
     CDS             complement(4615..>5109)
                     /locus_tag="AC158Q_02320"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR3338632.1"
                     /translation="AAINPGNSGGALVDADGNLVGVNTAIYSQTGGSMGIGYAIPVST
                     ARQVMEQIIQKGSVTRGWVGVGVQDVTREVAESFKLPVARGVLITQVERSGPADKAGV
                     KLGDILLAVNDTPVADTIGMLNMIAAMQPGQQARLKLTRSQTESELTVTIGRRPPPPA
                     RKGQ"
BASE COUNT          928 a         1552 c         1752 g          877 t
ORIGIN      
        1 gattgcgtgc gcgagatgga tgagcgggga ttgcgagacg cggtgctgga ggcggcgaga
       61 gtcaagcctt tcctggggat ctgcatcggc ctgcagatgc tgtttgaccg cagcgaggaa
      121 ggcgacacgc cgggactggg gctgctgccc gggcgggtgc ggcgtttccc ggcgcagggc
      181 atggtcgatg cgcaaggcgc gcgcctcaaa gtgccgcaca tgggctggaa cgaagtgcgg
      241 cagaccgccg cgcacgcctt gtgggacgga attccgcagg gcgagcgctt ttatttcgtg
      301 cacagttatt tcccggaacc cgcgagccgc ggggttacgg cgggcgaggc cgtctacggc
      361 gtgccgttcg cctgcgccgc ggcgcgcgac aacgttttcg cggtgcaatt ccaccccgaa
      421 aaaagcaaca cggcgggatt gaggctgctc gccaatttcc tggcgtggca gccggccgcg
      481 ccgaacccgc ggcagagaac cgccgccacc cccgccgcct agcgtactag agaaccatgc
      541 tgatcattcc cgcaatcgac ctgaaggacg gcaagtgcgt gcgcctgaag caaggcgcga
      601 tggacaaggc caccgtgttc tccgacgacc ccgtggcgat ggcccggcaa tggctcaagc
      661 agggcgcgcg gcggctgcac gtcatcgatc tgaacggcgc cgccgccgga cgcccgaaga
      721 acgagccggc gatcagggcg atcgcggccg cggtgggcga caagctgccg attcagctgg
      781 gcggcggcat tcgcgacctc gacaccatcg agcagctgat cgattccggc gtgggctacg
      841 tcattatcgg caccgcggcg gtgaagacgc ccggcttcct gcatgaggcg tgcacggcgt
      901 tcccgggacg cgtgctggtc gcgctcgacg ccaaggacgg caaggtcgcg gtggacggct
      961 ggtcgaagat gaccggtcat gaagtcctcg acctcgcgaa aaaattccag gactacggcg
     1021 tggaagcgat catttacacc gacatcggac gcgacggcat gctgaccggc gtcaacatcg
     1081 aggcgacggt gacgctggcg agggcgctct cggtgccggt gatcgcgagc ggcggcatca
     1141 acggtctcga cgacgtcaag gcgttgtgca aggtcgagca cgaaggcatc accggcgcca
     1201 tcaccgggcg cgccatctat gaaggcaagc tggatttcga gaaggcccag aagctggcgg
     1261 accagatctc caagaagggt gaatcgtgaa tcgtgaatag tgaatggaag gtcgaattgg
     1321 cctctgttca ccattcacca ttcaccgttt ctccgatggg cctcgccaag cgcatcattc
     1381 cctgcctcga cgtcaccgcc ggccgcgtgg tgaaggggat caacttcgtc ggcctgcgcg
     1441 acgcgggcga tccggtggag gttgcgcggc gctacaacga acaaggggcg gacgaactcg
     1501 tttttctcga tatcacggcc tccagcgacg ggcgcgatct catcgtgagc gtcgtcgagg
     1561 cggtggccgc ccaggtcttc atcccgctga ccgtgggcgg cggcgtgcgc gcggtggaag
     1621 acgtgcggcg tctgctcctc gccggcgccg acaaggtcag catcaacacc gcggcggtcc
     1681 agaacccgcg gctcgtgaag gacgcgacgg cgcgcttcgg ttcgcaatgt atcgtggtgg
     1741 cggtggacgc gcggcgcgtg cccggagagg gggcgccgcg ctgggaggtg tacacccacg
     1801 gcgggcgcaa tccgaccggg ctcgacgcgg tggagtgggg ccgcacgatg gaggccgccg
     1861 gagccggcga gatcctgttg accagcatgg atcgcgacgg cacccgcaat ggcttcgacc
     1921 tcgagctgac ccgcgcgttt tccgatgccc tctcgattcc gctgatcgcc agcggggggg
     1981 tgggcaatct cgagcacctg gccgaaggca taatccaggg ccgtgcggac gcggtgctgg
     2041 ccgcgagcat ttttcactac ggcgagtaca cggtgcgcca ggccaaggag cacatggcgc
     2101 ggcggggcat cgaagtaaga ctgtagtttc gagttacagg ttgcgagttt cgagttggag
     2161 ttgacagctg ccggtttttc gccgaacttg aaacttcaaa ctggaaactg gaaactgggc
     2221 ggatgaagga tgattggctc gaccaggtga agtgggcgca ggacggcctg gtgccggcgg
     2281 tgacgcagga gtccggcagc gggcgcgtgc tcacggtggc ctggatgaac cgggaggcgc
     2341 tcgccaagac cgtggagacg ggcgaggcgc actactggtc gcgctcgcgc aagaaattat
     2401 ggcgcaaggg cgaggagtcc gggcacgtgc agcaggtgag ggccatccgt ctcgactgcg
     2461 acgaggatgt ggtattgctg gaagtcgagc aggcgggcgg catcgcctgc cataccgggc
     2521 gccacagctg cttctaccgg aagctggacg gtggcaaatg gattatcacc gacccggtgc
     2581 tgaaggatcc cgggggaata tataaaaaag catgaataag cacgaatcag catgttaagc
     2641 atgaacaggc gatgacgaac gcggagatac tgaaacgatt gggtgaagtg ctcgcggcgc
     2701 gcagacaggc cgacccctcg tcgtcctacg tggcgagcct cttcgccaag ggcgaggacg
     2761 cgatcctgaa gaagctcgcg gaggaggccg ccgaaacggt gctggccgcc aagggcggtg
     2821 ataaactgca aatcgtgcgc gaaaccgccg atctgtggtt ccacagcctg gtgctgctcg
     2881 catggcacgg gctgaagccc gaggacgtgc tcgccgaatt gcggcggcgc gaaggcattt
     2941 ccggtattga cgaaaagaaa tcccgcaagt aggcgggatt tcctgaggag agcgaaatgg
     3001 gttcattcag catctggcac tggctgatcg tcctgctggt ggtcatattg attttcggca
     3061 ccaagaagct gagcaacctg ggcaaggacc tcggcagcgc ggtaaaaggc ttcaaggacg
     3121 gcatgaagcc cgaggacgac aagtccgcgc aggccaagac ggagatcccg cccaccaccg
     3181 gccagaccat cgagggcgag gccaagaaag aaacgcccaa ggcgtgaggg gtgaggggtc
     3241 aggcgtgagg cgcaagccgg cagccccccg gtttccgctc ctcacgcctc acgcctcact
     3301 cctcacctat gtttgatatc ggattctccg agctgctgct gatcgcggtc gtggcgctga
     3361 tcgtgatcgg tcccgagcgc ctgcccaagg tcgcgcgcac gctgggccat ctgttcggcc
     3421 gcatgcagcg ttacgtcaac gacgtcaagg ccgacatctc gcgcgagatg gagctggagg
     3481 agctcaagaa gatgcagtcg agcatggagg aaaccgcgcg ctcgatgcgc gattcggtcg
     3541 agcgcggcat ttccgaaacc gaaggcgagt tgaacaaggt cgcgcagtcc gcgcagccga
     3601 aaatcgatcc gtacgacgac ccgtttgccg ataacccgcc gccggaggct gcaccggcgt
     3661 cgccgcaggg cgagctgccg ctggaaccgg gccaacagcc gcccgggcag aaacaggcgt
     3721 gaagaaagtg tcgaggaatg agtgtcgggt gattacaacg tgaaagccgc tccgcccctc
     3781 gacgctcgtc acccgacact cgtcacgcgc cagccatgac ggaagacacc ttcatctccc
     3841 acctgatcga gctgcgcgac cggctgctgc gcgcgctgat tgccgtgggc gtgatattcc
     3901 tgatcctgtt tccctttgcc tccgagctct atgacctgct ggcttacccg ctgatgcgcg
     3961 cgttgccgga ggggtcgaag atgatcgcca cgggcgtgat cacgccgttc ctgatcccgg
     4021 tgaagatcgc cgtgctgatg gcgttcgcgg tggcgcttcc gtacgtgctc taccaggcct
     4081 gggccttcat cgcgccgggc ctctacgcgc acgagaggcg gctggtgata cccctggttg
     4141 tgacgagcac gctgctgttc ttcgtcgggg tggcgttctg ctacttcatc gtattcggcg
     4201 tggttttcga tttcatctac cagatcgcgc cgaagagcat ctcggtcgca cccgatatcg
     4261 agaattacct gaatttcgcg atcaccctgt tcctcgcatt cggggcgacc ttcgaggtgc
     4321 cgatcgtggt gctgatcctg gtccggatgg ggatggtcac gatcgagcag ttgaggagca
     4381 tccgtcccta cttcatcgtc ggggcgttca ttctcgccgc gatcatcacg ccgccggacg
     4441 tgatctcgca gttgttgctc gcgatcccga tgtgcctgct ctacgaggtc ggaatcattg
     4501 cggcgatgct gttcggaagg gcgcctccca aggcagccga agacggggca ggggacggaa
     4561 tggggcagga gcccggcgcc aagccttgac ggatgcgggg ctccgcgccc tttgttactg
     4621 ccctttccgc gccggcggtg ggggtctgcg gccgatggtg accgtgagct cgctctcggt
     4681 ttgactgcgg gtcaacttga gtcgggcctg ctggcccggc tgcatcgcgg cgatcatgtt
     4741 gagcatgccg atggtgtccg ccaccggcgt gtcgttgacc gcgagcagga tatcgccgag
     4801 cttcacgccg gccttgtctg cagggccgct gcgctccacc tgggtgatca gcacgccgcg
     4861 cgccacgggc agcttgaacg attccgcgac ctcccgggtg acgtcctgca cgcccacgcc
     4921 gacccagccg cgcgtcaccg agcccttctg gatgatctgc tccatcacct gccgcgcggt
     4981 gctgaccggg atcgcgtagc cgatgcccat cgagccgccg gtctgggagt agatcgcggt
     5041 gttgacgccg accaggttgc cgtccgcgtc caccagcgct ccgcccgaat tgccgggatt
     5101 gatcgcggc
//
LOCUS       DBNGSG010000091         3944 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3425811, whole genome shotgun sequence.
ACCESSION   DBNGSG010000091 DBNGSG010000000
VERSION     DBNGSG010000091.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3425811"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..1075
                     /locus_tag="AC158Q_02325"
     CDS             107..1075
                     /locus_tag="AC158Q_02325"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HIR3338633.1"
                     /translation="MKTTFLEFEQPIAELEAKIEELRFVQDDSAVDISGEIARLQKKS
                     AALTKEIYGKLSAWQIALVARHPQRPYTLDYVNNLFTGFEELHGDRGFADDPAIVGGL
                     ARFNGETCVVIGHQKGRDTKEKIHRNFGMPKPEGYRKALRLMKLAEKFGVPVFTFVDT
                     PGAYPGVGAEERSQSEAIGRNLYVMAELKVPLICTIIGEGGSGGALAIAVGDAVLMLQ
                     YSTYSVISPEGCASILWKSADYAAEAAETLGITSTRLKALGLIDRIVAEPPGGAHRDP
                     DTMMQNLKKSLQESWRQLKETPLDGLLENRFEKLVGYGKFKEVEQK"
     gene            1158..2540
                     /gene="tilS"
                     /locus_tag="AC158Q_02330"
     CDS             1158..2540
                     /gene="tilS"
                     /locus_tag="AC158Q_02330"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIR3338634.1"
                     /translation="MGGVAALVAAQLKDVIKPSARLVVGLSGGVDSVVLLDCLKRASR
                     ALRFRLAALHVNHQLNPNAARWSAFCRRLCRARGIPFESVTVTVRRGDGPEAAARAAR
                     YDVFTRQDCDFIALAHHRDDQVETVLIQLLRGAGVKGLAAMPFLRREEGRGPRIEGAS
                     PDTRHRTLDTRHSTPGILRPLLDATREEILEYAKQQELKWIEDESNADVQFGRNYLRH
                     EVLPAIARRFPSYRAAIARSARHLAEAADILDAAAAADGAGHLRNGTLAVEGLRRLPA
                     ARARNLLRWFLAGHDVAMPTTERLDEALRQMIGAKQDARVAIGLKGAKLQRYKDCIHV
                     VRAQKLPRPRSRRWRGEKCLELPEFDGTLSLKRSAGRGISLARLEGRAVTVRLRSGGE
                     RLQPDCRRPRRSLKNLLQELGMPPWRRERLPLIYCGERLVWAAGIGVDCAFQAQSAEP
                     ALFPEWLPSG"
     gene            2732..3868
                     /locus_tag="AC158Q_02335"
     CDS             2732..3868
                     /locus_tag="AC158Q_02335"
                     /inference="COORDINATES: protein motif:HMM:NF015445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR3338635.1"
                     /translation="MDRKQLNRIGVLAAGAVFGAALAVTGVSSPAYAQDKPQKVILRF
                     AADFPPPPHPAGLAMKYFADRLPKIIPGSEARLYYAGALYTVPEAFEAMRQGNLEMTW
                     MQIGKAAPVDSWMLSVVGPGILTTVGAVDNLDKTATYQMLVDRMLKNQQVKVFGVGHM
                     SFGMGVGGKQRYAKPGDFTGKKVRSMGPAENPVLASWKANPVVMGFGEVPSALESGVI
                     DGLMTSLGGWNSVREQAPYYTMGGAGAFVGDYYMISASRRWWDRLPAATRTALEGLIK
                     ETIQVQKEYNWCFDKMAYDKYGTKDPSKPGVYWMTPQEVSAMVNAMGDAAVRYVKSKT
                     PKEASPWVDSFVKEGRELSLQNPPGSSWIEKVDCSKHAAKIVIK"
BASE COUNT          736 a         1227 c         1347 g          634 t
ORIGIN      
        1 gctgaaatga cttggattca gcgttttctg ttcggtgcgc ttccgtgtgc ttccgtgtga
       61 ttccgtggct gattgggttt gagctgctgt atcatttggc atggccatga aaacgacttt
      121 cctagaattc gagcagccga tcgccgagct cgaagcaaag atcgaggagc tgcgcttcgt
      181 ccaggacgat tccgcggtgg acatttccgg ggagatcgcg cgcctgcaaa aaaagagcgc
      241 ggcgctgacg aaggaaatct acggcaagct ctcggcctgg cagatcgcgc tggtggcgcg
      301 ccacccgcag cggccctaca cgctcgatta cgtgaacaac ctcttcaccg gcttcgagga
      361 gttgcacggc gaccgcggct tcgcggacga ccctgcaatc gtcggagggc tcgcgcgctt
      421 caacggcgag acctgcgtcg tgatcggcca ccagaagggg cgggacacca aggagaaaat
      481 ccatcgcaac ttcggcatgc ccaagccgga ggggtaccgc aaggcgctgc gtctcatgaa
      541 gctggcggag aaattcggcg tgccggtctt caccttcgtc gacacgccgg gcgcctaccc
      601 cggtgtcggg gcggaggagc gcagccagtc cgaggcgatc ggccgcaacc tctacgtgat
      661 ggccgagctc aaagtgccgc tcatctgcac catcatcggt gaaggcggct ccggcggcgc
      721 cctggctatc gcagtgggcg acgcggtcct catgctgcaa tactcgacct actcggtaat
      781 ctctcccgaa ggctgcgcct ccatcctgtg gaagagcgcg gattacgcgg cggaggcggc
      841 cgagacgctc ggcatcacct cgacgcggct gaaggcgctg ggcctgatcg accggatcgt
      901 ggccgaaccg cccggcggtg cgcaccgcga ccctgacacg atgatgcaga atctcaagaa
      961 atcgctgcag gagtcctggc ggcagctcaa ggagaccccg ctcgacgggc tgctcgagaa
     1021 ccgcttcgag aaactcgtcg gctatggcaa gttcaaggaa gtcgagcaaa agtaaccccc
     1081 cctatattcc cccccttaag aaaggggggg acgcagcagg gggggtcaat cccccccttg
     1141 gcaagagggg gaacaacgtg gggggggtgg ctgcgctcgt cgccgcgcag ctcaaggatg
     1201 tcatcaagcc atcagcccgc ctggtcgtcg gactgtccgg cggcgtcgat tcggtcgtcc
     1261 tgctggattg cctgaaacgc gcttcgcgcg cgctgcgttt ccggctcgcc gcgctgcacg
     1321 tcaatcacca gttgaatccg aacgccgcgc gctggtcggc gttctgccgg cggctgtgcc
     1381 gcgcgcgcgg catcccgttc gaaagcgtga cggtgacggt gcggcggggc gacggtcccg
     1441 aggcggcagc gcgggcagcg cgctacgacg tcttcacgcg ccaggattgc gatttcatcg
     1501 ccctcgcgca ccaccgcgac gaccaggtgg aaacggtcct gatccagctc ttgcggggcg
     1561 cgggcgtgaa ggggctcgcg gccatgccgt tcctgcggag ggaggagggt cgagggccga
     1621 ggatcgaggg ggcaagtccc gacactcgac accggacact cgacacccga cactcgacac
     1681 cgggaatcct ccggccgctg ctcgacgcga cacgggagga aatcctcgag tacgcaaagc
     1741 agcaagaatt gaaatggatc gaggacgaga gcaatgcaga cgtccaattc gggcgcaact
     1801 atctgcggca cgaagtgctg cccgcaatcg cacggcgctt cccgtcgtac cgcgcggcga
     1861 tcgctcgttc cgcacgtcat cttgccgagg cggctgacat actggacgca gccgccgctg
     1921 cggacggggc cggccatctg cgcaacggga cgctggcggt cgaggggctg cgccggctgc
     1981 cggcggcgcg cgcacgtaat ttgttgcgct ggtttctcgc aggccacgac gtggccatgc
     2041 ccacgacgga gcgcctggac gaagcgctgc gccagatgat cggggcgaag caggatgccc
     2101 gggtcgcgat cgggctgaaa ggcgcaaaac tgcagcgcta caaggactgc attcacgtgg
     2161 tgcgggcgca gaagttgccc cggccccgaa gccggcgctg gcggggggaa aaatgcctgg
     2221 aactgcccga gttcgacggc actctcagtc tcaagcgcag cgcaggccgc ggcatcagcc
     2281 tcgcccgact ggaaggccgc gcggtgacgg tcaggctgcg cagcggcggc gagcgcctgc
     2341 agcccgattg ccggcgcccg cggcgcagcc tcaagaacct gctgcaggaa cttggcatgc
     2401 cgccctggag gcgcgagcga ttgccgttga tttattgcgg cgagcggctg gtgtgggccg
     2461 ccggcatcgg cgtggattgc gcgttccagg cgcaaagcgc ggagcccgcg ctattcccgg
     2521 aatggctgcc atcgggatag acgtttgtgg ccagcgcgac ctgcatagat gggctgcgcg
     2581 atagcgggtc atggcgtggc gccctgatcc gcgaacggtg ccctcaccgg gagccttgac
     2641 gctgccaacc cgttgatata cggtgcccag caacaagaag tgaggatcgg ttgaatcagc
     2701 atccgtaact ataaacagga taggaggagt catggatcgt aagcaactga atcgaatcgg
     2761 cgtattggcc gctggcgcgg ttttcggggc ggcactggcc gtcaccgggg tgagctcgcc
     2821 ggcttacgcc caggacaagc cgcagaaagt cattttgcgc ttcgcggctg acttcccgcc
     2881 gccgccgcac ccggcgggac tggccatgaa gtatttcgcc gatcgcttgc cgaagatcat
     2941 ccccggcagc gaggcgcgct tgtattacgc cggcgcgctc tacaccgtcc ccgaggcctt
     3001 cgaggcgatg cgccagggca acctggaaat gacctggatg cagatcggca aggcggcccc
     3061 ggtcgattcc tggatgttgt cggtcgttgg tcccggtatc ctcacgacgg tcggcgcggt
     3121 agacaacctg gacaagaccg cgacctacca gatgctggtc gaccggatgc tgaaaaacca
     3181 gcaggtcaag gtgttcggcg tcgggcacat gagcttcggc atgggcgtgg gcggcaagca
     3241 gcgctacgcc aagcccggcg acttcaccgg caagaaggtg cgcagcatgg ggcccgccga
     3301 gaatcccgtg ctcgcgtcgt ggaaggcaaa tccggtcgtc atggggttcg gcgaggtgcc
     3361 gagcgcgctg gaatccggcg tcatcgacgg gctgatgacc agcctcggcg gctggaacag
     3421 cgtgcgcgag caggcgccgt actacaccat gggcggggcc ggcgcctttg tcggcgatta
     3481 ttacatgatc agtgcgagcc gccgctggtg ggaccgtctt ccggcggcca cccgcaccgc
     3541 gctggaggga ctcatcaagg aaactatcca ggtgcagaag gaatacaact ggtgcttcga
     3601 caagatggcc tacgacaagt acggcaccaa ggacccatcg aagccgggcg tctactggat
     3661 gaccccgcag gaggtctccg ccatggtgaa cgcgatgggc gacgcggcgg tgcgctacgt
     3721 gaagagcaag acgccgaagg aagccagccc atgggtcgac tcgttcgtca aggaaggccg
     3781 cgaactgtcc ctgcagaacc cgcccggctc gtcctggatc gagaaggtcg actgctcgaa
     3841 gcacgccgcc aagatcgtga tcaagtgaag tccccgggcg atcgcaccgc tggctattcg
     3901 gcctgctgag gcggaaaggc ggcccgggtt cgctcgtgga gaag
//
LOCUS       DBNGSG010000092         4104 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_31195, whole genome shotgun sequence.
ACCESSION   DBNGSG010000092 DBNGSG010000000
VERSION     DBNGSG010000092.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4104)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_31195"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..1957)
                     /locus_tag="AC158Q_02340"
     CDS             complement(113..1957)
                     /locus_tag="AC158Q_02340"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HIR3338636.1"
                     /translation="MNQPATAARLAKSSDAPAYVKHGRLKAWVEEIARLTKPDRVYWC
                     DGSQEEYDRLCGEMVATGMLIKLNPQKRPGCFLARSDPDDVARMEERTFVCSKNKEDA
                     GPNNNWLAPDEMRATLGKLFDGCMRGRTMYVIPYSMGPLGSHIAHIGVEITDSPYVVV
                     NMKLMTRIGRKVLDVMGANGYFVPCVHSVGAPLAPGQKDSAWPSNKEQKYIVHYPETK
                     EIWSFGSGYGGNALLGKKCLALRIASIMAKEQGWLAEHMLILGIETPEKKKHYIAAAF
                     PSACGKTNLAMLVPPKVFRDKGWKITTIGEDIAWIKPGADGRLYAINPEAGAFGVAPG
                     TSEFTNPNCLKAVTHDTIFTNVALTPDGDVWWEGLSKEPPGELIDWQGKAWTPGGGRK
                     AAHPNARFTMSCAQIPSVDPDWENPRGVPISVFLFGGRRATAVPLVTESFDWTHGVYL
                     ASTMASETTAAIVGQVGVVRRDPFAMLPFCGYHYGDYFRHWLSIGKSLKHPPRIFGVN
                     WFRQDAQGEFIWPGFGDNMRVLKWTIDRIEGKADAVKTPLGLQPRYEDLEWAGLESFS
                     RARFEELTKVDPTAWRKELEDHTELFEKLKSRMPKELYEQRDRLERAL"
     gene            complement(2126..2827)
                     /locus_tag="AC158Q_02345"
     CDS             complement(2126..2827)
                     /locus_tag="AC158Q_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011545808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HIR3338637.1"
                     /translation="MIDVYSWPTPNGHKIHIMLEETGLRYRVHGVNIREGDQFKPEFL
                     KISPNNRIPAIVDSEGPNGKPISLFETGAILLYLASKTGKFLPADLHARWSCVQWLMW
                     QMGGLGPMMGQANHFRRYSKEKIQYAIDRYSNEANRLTRVLDTRLGEARYTAGEEYSI
                     ADMACFPWLRGAETRGVKLDEYPNVKRWFDEINARPAVQRALKTLAEAQAANTAPHSD
                     KSWDILFGNTQFQKR"
     gene            2951..>4104
                     /locus_tag="AC158Q_02350"
     CDS             2951..>4104
                     /locus_tag="AC158Q_02350"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3338638.1"
                     /translation="MSNSTSWLLPPGTSPDAALILTARGIRAFTDGFVALLLPYYLTL
                     LGYSALEVGVIATATLLGSGLLTLLVGVFAYRFRLHILLTTACLLMLGTGIGFAAVTD
                     FLPLLLISVIGTLNPSSGDVSVFLPLEQTLLSHAATSQHRTALFSRYSLVGQLVGALG
                     ALAAAAPGILAQWDILELKAGIQVMFILYGLSALVALALYRRLASARSQGEEVRAAPL
                     GPSRGVVYRLAALFSLDAFAGGFAVQSLLALWLFLRFDLSVEAAGTIFFWTGILAAAS
                     FLAAPAIARRIGLVNTMVFTHLPANVFCVLVPFMPTLPLALLFLFLRAALSSMDVPAR
                     TSYVMAVVSPAERPAAAGMTAVPRSLAAALSPAFAGYLLTLSPFGWPLVI"
BASE COUNT          618 a         1334 c         1258 g          894 t
ORIGIN      
        1 cgttcgaggc gatcatccag tcgatggaca cccaaaagcc ggtgtccctc gatcgataga
       61 tacatgaaaa aagcgagccg ggtggctcgc ttttatctgg agcgcgctcg ccctagagag
      121 cgcgttccag ccggtcgcgc tgctcgtaga gctctttcgg catgcgcgac ttgagcttct
      181 cgaacagttc cgtatggtcc tccagttcct tgcgccacgc cgtgggatcc accttcgtca
      241 gttcctcgaa gcgcgcgcgg ctgaaactct ccagccccgc ccattccagg tcctcgtagc
      301 gcggctgcag gcccagcggc gttttcaccg cgtcggcctt gccctcgatg cgatcgatcg
      361 tccacttcaa tacgcgcatg ttgtcgccga aacccggcca gatgaattcg ccttgcgcgt
      421 cctgccggaa ccagttgacg ccgaagatcc tgggcgggtg cttgaggctc ttgccgatcg
      481 acagccagtg tctgaagtaa tcgccgtagt ggtagccgca aaacggcagc attgcgaacg
      541 gatcgcgccg caccacgcct acctgcccga cgatcgcggc ggtcgtttcc gatgccatcg
      601 tcgaggccag gtacaccccg tgcgtccagt cgaacgattc tgtcaccagc ggcaccgccg
      661 ttgcacgccg cccgccgaac aggaatacgc tgatcggcac cccccgcggg ttctcccaat
      721 ccgggtccac ggacggaatc tgggcgcagc tcatggtaaa tcgcgcgttg gggtgcgccg
      781 ccttgcgacc cccgccgggc gtccaggcct tgccctgcca gtcgatgagt tccccgggcg
      841 gctccttgct caggccttcc caccacacgt cgccgtccgg cgtcagcgcg acattggtga
      901 atatcgtgtc gtgcgtgacc gctttcaggc aattgggatt ggtgaactcg gaggttcccg
      961 gcgcgacgcc gaaggcgccg gcctcggggt tgatggcgta gaggcggccg tcggctccgg
     1021 gcttgatcca ggcgatgtct tcgccgatgg tcgtgatctt ccagcccttg tccctgaaca
     1081 ccttcggcgg caccagcatc gcgaggttgg tcttgccgca tgcgctcggg aacgccgctg
     1141 cgatgtaatg cttcttcttc tcgggggtct cgatgccgag gatcagcatg tgctcggcga
     1201 gccagccctg ttccttcgcc atgatcgagg cgatgcgcag ggcaaggcac ttcttgccga
     1261 gcagtgcgtt gccgccgtag ccgctgccaa atgaccagat ttccttggtc tctggataat
     1321 ggacgatgta cttctgttcc ttgttgctgg gccaggcgct gtccttctgc cccggggcca
     1381 gcggtgcgcc gacggagtgc acgcacggca cgaagtagcc gttggcgccc atcacgtcga
     1441 gcaccttgcg cccgatgcgc gtcatcaatt tcatgttgac gacgacgtag ggcgagtcgg
     1501 taatctcgac accgatatgg gcgatgtgcg agccgagcgg ccccatgctg taggggatca
     1561 cgtacatcgt acgcccgcgc atgcagccgt cgaacagctt gccgagcgtc gcccgcatct
     1621 cgtccggcgc cagccagttg ttgttggggc ccgcgtcttc cttgttcttg ctgcacacga
     1681 aggtgcgttc ctccatgcgc gcgacgtcgt cgggatcgga acgcgcgagg aagcagccgg
     1741 gccgcttctg cgggttcagc ttgatcagca tgccggtggc gaccatctcg ccgcaaagcc
     1801 ggtcgtattc ctcctgcgag ccgtcgcacc agtacacccg gtccggcttg gtgagtcgcg
     1861 cgatttcctc cacccacgcc ttcagcctgc cgtgcttgac gtaggcaggc gcgtcggagg
     1921 acttggcgag gcgggccgcg gtggcaggtt gattcatata actcctctcc ggtgcgcgga
     1981 attagtcttt taaaaacaaa ggcaaagcac ctatttcagg ccgccttgcc gtttagctgg
     2041 attttacacg aaatccacct cgctctgaat cacgggaacg ctcgtggatt ccggttggtt
     2101 tatgcccttc gagccaacag attcctcatc gtttctggaa ctgcgtattg ccgaacagga
     2161 tgtcccacga cttatcgctg tgtggcgcgg tattggcggc ttgcgcttcg gcaagcgtct
     2221 tcaacgcgcg ctgaacagcg gggcgcgcgt tgatctcgtc gaaccagcgc ttgacgttcg
     2281 ggtattcgtc gagtttgacg cctcgggtct ccgcgccgcg cagccaggga aagcaggcca
     2341 tgtctgcaat cgagtactcc tcccctgcgg tgtagcgcgc ttcgccgagc cgggtgtcga
     2401 ggacccgcgt cagccggttc gcctcattgc tataacggtc gatcgcgtac tggatttttt
     2461 ctttcgagta gcgccgaaaa tggttggcct ggcccatcat gggccccagg ccgcccattt
     2521 gccacatcag ccattgcacg caggaccagc gcgcatgcag gtccgccggc aggaattttc
     2581 cggtcttgga ggcgaggtag agcaggatcg ccccggtttc gaacaaggat atcggcttgc
     2641 cgttcgggcc ctcgctgtcc acgatcgcgg gaatgcggtt gttcgggctt atcttgagga
     2701 attccggctt gaactggtcg ccctcgcgga tattgacgcc gtgaacgcgg taccggagcc
     2761 cggtctcttc cagcatgatg tggatcttgt gtccgttcgg cgtgggccag ctgtagacgt
     2821 cgatcatagg gcctcccgca atatcgtgat gtgtgatgag cgatgggtga cgcgtgatga
     2881 gagaaagcaa atcctaccat catcatgaca gcgccagacg cgcgccgttc ctgttagtct
     2941 tgacacagat atgtcaaatt ccacatcatg gcttctgccc cccggaacct cgcccgatgc
     3001 ggcgctgatc ctcacggcgc gcggcatccg cgcatttacc gacggcttcg tcgcgctgct
     3061 gctgccgtac tacctcacgc tgctgggtta cagcgcgctg gaggtgggcg tgatcgccac
     3121 ggcaacgctt ctcggctccg gactgctgac gttgctcgtg ggcgtattcg cctaccgttt
     3181 ccgattgcac attctgctca ccaccgcgtg cctgctgatg ctcgggaccg gtatcggctt
     3241 tgcggcggtc acggactttc tgcctttgct gctgatctcg gtgatcggga cactcaatcc
     3301 atcgagcggc gatgtcagtg tattcctgcc tctcgaacaa accttgttgt cgcacgccgc
     3361 gacgagccag catcgcaccg cgctgttctc gcgctacagc ctggtggggc aacttgtcgg
     3421 tgcgctgggc gcgctggctg ctgcggctcc cggcattctc gcgcaatggg acatcctcga
     3481 gctcaaggcg gggattcagg tgatgttcat cctgtacgga ctctcggccc tcgtcgcgct
     3541 cgcgctctat cgccgcctcg cctcagcccg ttcgcaggga gaggaagtgc gcgcggcgcc
     3601 gctgggaccc tcgcggggcg tcgtctaccg gctcgcggcg ctgttcagcc tggacgcgtt
     3661 cgctggcggt ttcgccgtgc agtcgctgct tgcgctgtgg ctcttcctcc ggttcgacct
     3721 gtcggtggaa gccgccggta ccatcttttt ctggaccggc atcctggcag ccgcttcctt
     3781 tctcgccgcc ccggcgatcg cgcgtcgcat cgggctcgtc aacaccatgg tgtttaccca
     3841 cctgccggcg aacgtttttt gcgtgctcgt tcccttcatg ccgacgctgc ccctcgcctt
     3901 gctgttcctg ttcctccgag cagcgctttc gtcgatggac gtgccggcgc gcacttccta
     3961 cgtgatggcg gtggtttcac cggcggagcg cccggcggcg gcgggaatga ccgccgtgcc
     4021 gcgcagcctc gcggccgcgc tgagcccggc gttcgcgggc tacctgctca cgctttcgcc
     4081 tttcggctgg ccactggtaa tctg
//
LOCUS       DBNGSG010000093         4033 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_710451, whole genome shotgun sequence.
ACCESSION   DBNGSG010000093 DBNGSG010000000
VERSION     DBNGSG010000093.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4033)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_710451"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1176)
                     /locus_tag="AC158Q_02355"
     CDS             complement(<1..1176)
                     /locus_tag="AC158Q_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR3338639.1"
                     /translation="MLIEFFLKLKEGGVPVSIKEFLTLIEALAKRVAFGSMEEFYYLA
                     RAALVKDEKYYDRYDRVFAAYFKGIVSADGVELLIPEEWLKKLAEKILTEEDKKLIES
                     LGGWDKLMETLKKRLEEQKGRHQGGSKWIGTAGTSPYGAYGYNPEGVRIGQPESRHRR
                     AVKVWDRREYRNLDDTVELGTRNIKVALRRLRKFARIGAPEELDLDDTIRSTAKNAGW
                     LDLKMVPERHNAVKVLLFLDVGGSMDDYIRICEQLFSAARVEFKHLEYFYFHNFLYER
                     VWRDNRRRAAERMATWDVLHTYPHDYKVILVGDATMSPYEIVYPGGSVEHTNEEAGAV
                     WLQRLLAVYPQAVWLNPQPESVWDFHESIRLTRQLMGERMFPLTLEGLDRGMRLLSRA
                     "
     gene            complement(1397..2242)
                     /locus_tag="AC158Q_02360"
     CDS             complement(1397..2242)
                     /locus_tag="AC158Q_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3338640.1"
                     /translation="MKRFEGTDTYIATDDLMMAVNAALTLERPLLVKGEPGTGKTMLA
                     IEIARALGRPLHEWHIKSTTKAQHGLYEYDAVSRLRDSQLGDPRVHDIRNYIVQGMLW
                     KAFTADSPAVLLIDEVDKADIEFPNDLLRELDRMEFYVYETQELVKARHRPLVLITSN
                     NEKELPDAFLRRCFFHYIRFPDHETMERIVAVHHPGIKKQLLQEALEAFFEIREVPGL
                     KKKPSTSELLDWLKLLMAEDISPEALHSQDSHKIIPPLHGALIKNEQDVHLFERLVFM
                     SRRKS"
     gene            2325..3047
                     /locus_tag="AC158Q_02365"
     CDS             2325..3047
                     /locus_tag="AC158Q_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338641.1"
                     /translation="MNGAPGTTRALWPLPLLGGLIPAMAALLALWLSFSLDLIAPCNP
                     FLDGCVSISRAARHDLPNHVFRALVLPAAMLQALTWLLCAGWIRELRGEARGMLRVLP
                     WLGVLAGVFLVLYGTFLGTEGQAYRWMRRFGIIFYFGFTFLCMLIVSGSLWRLANAGA
                     LLVPARLDRWLVALCAITLALGLIQVFTPPLLGSEDLKNRLENILEWYAALCFTLFFL
                     ALAWLWRHARFHARLTNGGSGP"
     gene            complement(3051..3638)
                     /locus_tag="AC158Q_02370"
     CDS             complement(3051..3638)
                     /locus_tag="AC158Q_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013689749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR3338642.1"
                     /translation="MTRNQKNIVAVLLAAGAGRRFGGDKLLHPLEDGVAIAAHAARNL
                     ITAGLEVVAVVRWGDFPLYDMMEQEGCQVTMFQGAARGMGASLAHGIAQARGADGWVV
                     ALADMPRIAPATIRRIAAELEAGALIAAPARKGERGHPVGFGAGLRDELVALDGDQGA
                     RAVIERRRDDLKLFECDDPGILLDIDRKDDLASVG"
     gene            3811..>4033
                     /locus_tag="AC158Q_02375"
     CDS             3811..>4033
                     /locus_tag="AC158Q_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HIR3338643.1"
                     /translation="MPKFCANLTMLWNELDFMDRFAAAAKAGFKGVEYLFPYDHDKGR
                     IAAELKKNGLAQALHNLPAGDWAAGERGIG"
BASE COUNT          690 a         1319 c         1216 g          808 t
ORIGIN      
        1 cgccctgctc agcaggcgca tcccgcggtc gagcccctcc agcgtcaacg ggaacatccg
       61 ctcccccatg agctgacggg tcaggcggat ggattcgtgg aaatcccaga ccgattccgg
      121 ctggggattc agccagacgg cctgtggata gactgcgagc agccgctgca gccacaccgc
      181 gccggcctcc tcgttggtgt gctcgacact gccgccggga tagacgatct cgtaggggct
      241 catggtcgcg tcgcccacga ggatcacctt gtagtcgtgg ggataggtgt gcagcacgtc
      301 ccacgtcgcc atccgctcgg ccgcccgccg gcggttgtcg cgccacaccc gctcgtagag
      361 gaagttgtga aaatagaaat attcgaggtg cttgaactcc acgcgcgccg ccgagaacag
      421 ctgctcgcag atgcggatgt agtcatccat ggaaccgccg acgtcgagga acaacagcac
      481 cttcacggca ttgtgccgct ccgggaccat tttcaaatcc agccagccgg cgttcttcgc
      541 agtcgagcgg atggtgtcgt cgaggtcgag ctcctcgggt gcgccgatgc gcgcgaactt
      601 cctcaaccgc ctcagcgcca ccttaatgtt gcgggtgccg agctcgaccg tgtcgtcgag
      661 gttacggtac tcgcgcctgt cccacacttt caccgcgcgg cggtggcgcg actcgggctg
      721 gccgatgcgc acgccctcgg ggttgtagcc gtaggcgccg taaggactgg tgccggccgt
      781 gccgatccac ttcgagccgc cctggtgacg gcctttctgc tcctcgaggc gctttttcag
      841 cgtctccatg agcttgtccc agccgccgag agactcgatc agcttcttgt cctcttccgt
      901 caggattttt tccgccagct tcttcagcca ctcttccggg atcagcagct cgaccccgtc
      961 cgctgaaaca atgcccttga agtaggcggc gaacacgcgg tcgtagcggt cgtagtactt
     1021 ctcgtcctta acgagcgcgg cgcgcgccag gtaatagaat tcttccatgc tgccgaaggc
     1081 gacgcgcttc gccagcgcct cgatcagggt caggaactcc ttgatcgaga ccgggacgcc
     1141 gccttccttc agcttgagaa aaaactctat taacacctta aaccttaaat tagccgccaa
     1201 gacgccaaga gcgccaagaa attcatcaac aaaaaacaag aacaatgtga ataattgagg
     1261 gtggttgtcc tgaagtggac aacggacagg agccacctca tttcaatcct gcgttccgcc
     1321 ccacttacat tccatgaatt cttagtatat tgatcttctt ggcgtgcttg gcgtcttggc
     1381 ggctattttc cggttttcac gatttccgcc gcgacatgaa gacgagccgc tcgaagagat
     1441 gcacgtcctg ctcgttcttg atgagcgcgc cgtgcagcgg cgggatgatc ttgtggctat
     1501 cctggctgtg cagcgcctcg ggcgaaatgt cctccgccat gaggagcttc agccagtcga
     1561 gcagctccga agtcgaaggc ttcttcttca gtcccggcac ttcgcggatt tcgaagaagg
     1621 cctccagcgc ctcctgcagc agctgcttct tgatgccggg gtgatgcacg gcgacgatac
     1681 gctccatcgt ttcgtggtcg ggaaagcgga tgtagtggaa gaagcagcgc cgcaggaacg
     1741 cgtcgggcag ttccttctcg ttgttgctgg tgatcagcac cagcggccgg tggcgcgcct
     1801 tgaccagttc ctgggtctcg tagacgtaga actccatgcg gtcgagttcg cgcagcagat
     1861 cgttggggaa ctcgatgtcc gccttgtcca cttcatcgat caaaagcacc gccggcgagt
     1921 cggcggtgaa ggccttccac agcatgccct gcacgatgta gttcctgatg tcgtgcacgc
     1981 gcgggtcgcc caattgagag tcgcgcagcc gcgagaccgc gtcgtactcg tagagcccgt
     2041 gctgggcctt ggtggtggac ttgatgtgcc actcatggag cgggcggccg agcgcccggg
     2101 cgatctcgat cgccagcatg gtcttgccgg tgccgggctc gcctttcacc agcagcggcc
     2161 gctccagcgt gagtgcggcg ttcactgcca tcatcaggtc gtcggttgcg atgtaggtgt
     2221 cggtgccctc gaatcgtttc atgtcgttcc cggcgttgat cgtatgaggc gagtctcgca
     2281 tccgtcccca ccaccgtcaa gaaatcgcgc taacatttca gtccatgaat ggcgccccgg
     2341 ggaccacacg agcgctttgg ccgctgccac tgctcggcgg gctgattccg gccatggccg
     2401 ccctgctcgc cctgtggctc tccttctcgc tcgacctcat cgcgccatgc aatcccttcc
     2461 tggacggctg cgtctcgatc agccgcgctg cgcggcacga tctcccgaac cacgtattcc
     2521 gcgcgctggt gctgccggcg gccatgctgc aggcgctgac ctggttgctt tgtgcgggat
     2581 ggatcaggga attgagaggg gaagcgcgcg gcatgctgcg cgtcctgccc tggctgggcg
     2641 tccttgccgg ggtgtttctg gtcctctatg gcaccttcct cggcaccgaa ggacaggctt
     2701 accgctggat gcggcgattc gggatcatct tctatttcgg gttcactttt ctgtgcatgc
     2761 tgatcgtcag cggctcgctc tggcgcctgg ccaatgccgg cgccttgctc gttcccgcgc
     2821 ggctcgatcg atggctggtt gcactgtgcg caatcacgct tgcccttggc cttattcagg
     2881 tattcacgcc ccccctgctg ggcagcgaag acctcaagaa ccgcctggaa aatatcctgg
     2941 aatggtacgc tgccctctgc tttaccctgt tcttcctcgc gcttgcctgg ctgtggcgac
     3001 acgcgcgctt ccatgcgcga ctgacgaacg gggggtccgg gccatgagaa tcagccgacc
     3061 gacgcaaggt cgtccttccg gtcgatgtcg agcaggatgc ccggatcgtc gcactcgaag
     3121 agcttcaggt catcgcgacg ccgttcgatg accgcgcgcg cgccctggtc gccgtcgagc
     3181 gccaccagct catcgcgcag tccggcgcca aagccgacag gatgcccacg ctcccccttg
     3241 cgggccggtg cggcgatcag cgcgcccgcc tccagctccg ccgcgatcct ccggatcgtg
     3301 gcgggcgcga tgcgcggcat gtcggccagc gccacgaccc agccatcagc accgcgcgcc
     3361 tgcgcgatgc cgtgggcaag gctcgcgccc atgccgcggg ctgcgccctg gaacatcgtg
     3421 acctggcagc cttcctgctc catcatgtcg tagaggggaa aatcacccca gcgcacgacc
     3481 gcgaccacct cgagacccgc cgtgatcaga ttgcgcgcgg catgggcggc gatcgccacg
     3541 ccgtcctcca gcgggtgcag cagcttgtcc ccgccgaaac gccttccggc gcccgccgcg
     3601 agcaggaccg cgacaatatt cttctgattc cgagtcatat cgccgccgag tataacggca
     3661 gcgccgcttg cgcccgtgaa acacatgacg ctggtgtcgg cacaagcctc gcgttccaga
     3721 agcaaagcag gggcaactgc gcacacaggc ggggtagttg gtgttactcc ctctataatg
     3781 cggcttcaac ccaggtgaat gaagccgcgt atgccgaaat tctgcgccaa cctcacgatg
     3841 ctctggaacg agctcgactt catggatcgc ttcgccgcgg ccgcgaaagc gggcttcaag
     3901 ggggtcgaat acctgtttcc ctacgatcac gacaaaggcc gtatcgccgc cgaactgaag
     3961 aagaacggac tcgcacaggc gctgcacaac ctgcccgccg gcgattgggc agcgggtgag
     4021 cgcggcatcg gct
//
LOCUS       DBNGSG010000094         3578 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1389801, whole genome shotgun sequence.
ACCESSION   DBNGSG010000094 DBNGSG010000000
VERSION     DBNGSG010000094.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1389801"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1892)
                     /locus_tag="AC158Q_02380"
     CDS             complement(<1..1892)
                     /locus_tag="AC158Q_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010101614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HIR3338644.1"
                     /translation="MQSPLQSVLILLAAAVLVVAVFRALRLPPMLGYLIVGVAIGPHA
                     LGWISETAEVRGLAEIGVVFLMFSIGLEFSLPRLFTMRRIVFGLGTAQVLATLLIVWG
                     IAAALDVEWRAGLILGGVLAMSSTAIVAKMLAERLELNSPHGRQIIGVLLFQDLAVVP
                     LLILVPALAAGTEDLASNLGLAFVKAVVVLSLMLFLGQRLMRGWFHLVARQKSSELFV
                     LNVLLITLGLAYITEIAGLSLALGAFVAGTLISETEYRYQVETDIKPLRDVLLGLFFV
                     TIGMLLDLRVVFEQAQWVFLALVILLALKALLIFGLSLAFGSDSAVALRTGLALASCG
                     EFGFVLLARADGLGLVTGQIMQTVLAAMLLSMLAAPFLVERGGWMARRWSRSDWMHRA
                     MELHNIAVRSMATDQHVVICGYGRSGQNLARFLERESIPFIALDVDPQRIREAATAGE
                     SVVFGDAARREVLVAAGLLRAKLLAITYSDTASALRILGYVQELRPGLPVVVRTVDDS
                     DIDQLKSAGAAEVVAELMEGSLMLASTTLMLAGVPLNRVLRRIRETREQRYSLFRGFF
                     RGITDESDSKDEASQPRLHSLTVAPQSAAIGRTLGELDLARLHVEVTAVRRRNIRGIA
                     PDPTTR"
     gene            2088..3092
                     /locus_tag="AC158Q_02385"
     CDS             2088..3092
                     /locus_tag="AC158Q_02385"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HIR3338645.1"
                     /translation="MKTASGGVRRAPQESAVLAMARDVLEIEAKAVSALIGRLDQGFV
                     QAVELLLGCRGRVVVSGIGKSGHIARKIASTLASTGTPAFYVHPAEASHGDLGMVARD
                     DVFIALSNSGESDELLAIVPLLKRQGAKLVALTGSPQSSLAREADVHLYAGAEKEACP
                     LNLAPTASTTAALALGDALAVALMQAKGFTRDEFAASHPGGPLGRKLLTHVRDVMRRG
                     KEAPGIGHAAMAMEGVLEMSRGRMGMTAVLDDQGRVIGILTDGDLRRALEKGFDLRSA
                     RIAEIMTRGPRTIGEDKLAAEAVEIMERFKVNQLLVVDRDQKLLGALNMHDLFRAKVI
                     "
     gene            3195..>3578
                     /locus_tag="AC158Q_02390"
     CDS             3195..>3578
                     /locus_tag="AC158Q_02390"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KdsC family phosphatase"
                     /protein_id="HIR3338646.1"
                     /translation="MQTIYDRAKNIRVAAFDVDGVLTDGALYYADLGEELKRFSVHDG
                     HGMKMLKDCGIQLAIITSRRSRCVEERARNLGIELLYQGATDKLVAFRELLGRCRIEA
                     TACAYVGDDLVDLPVMKRCGLAVTVP"
BASE COUNT          669 a         1199 c         1188 g          522 t
ORIGIN      
        1 attcgcgtcg tgggatccgg cgcgatgccg cggatattgc gccgccgcac cgcggtcact
       61 tccacgtgca agcgcgcgag gtcgagctcg cccagcgtgc ggccgatcgc cgccgactgc
      121 ggcgcgaccg ttagcgagtg cagccgcggc tgggacgctt cgtccttgct gtcggattcg
      181 tccgtgatgc cgcggaagaa gccgcggaac aggctgtagc gctgctcccg ggtttcccgt
      241 atgcgccgca gcacacggtt caggggaacg ccggccagca tgagcgtggt cgacgccagc
      301 atgaggctgc cctccatcag ttcggcgacg acttccgcgg ccccggcgct cttcagctgg
      361 tcgatgtcgc tgtcgtccac ggtgcgcacc acgaccggca ggcccgggcg caactcctgg
      421 acgtagccca gtattctcag ggcggaggcc gtgtccgaat acgtgatcgc aagcagcttc
      481 gcgcgcagca gcccggccgc caccagcact tcgcgccgcg cggcgtcccc gaacaccacg
      541 ctctcgccgg ccgtcgccgc ctcccggatg cgctgcgggt cgacgtcgag cgcgatgaac
      601 gggatcgact ctctttccag gaagcgcgcc aggttctgcc cgctgcgccc gtagccgcag
      661 atcaccacgt gctggtcggt ggccatggac cgcaccgcga tgttgtgcag ctccatcgcg
      721 cggtgcatcc agtccgaccg gctccagcgg cgcgccatcc acccgccgcg ttcgacgaga
      781 aagggcgccg ccagcatcga gagcagcatg gcggcgagca ccgtctgcat aatctgcccg
      841 gtcaccagcc cgagcccgtc cgcgcgcgcg agcaggacga agccaaactc gccgcaggac
      901 gcgagcgcga gcccggtgcg cagggcgacc gcggaatccg aaccgaacgc gaggctcagg
      961 ccgaaaatga gcagggcctt caacgccagc agaatgacga gcgccagaaa aacccactgc
     1021 gcctgctcga atacgacgcg caggtcgagc agcatgccga tggtgacgaa gaacagcccc
     1081 agcagcacgt cgcgcaacgg cttgatgtcc gtttccacct ggtagcggta ctcggtctcc
     1141 gagatcagcg tgccggcgac gaaggcgccg agcgcgagcg acaggccggc gatctcggtg
     1201 atataggcaa gtcccagcgt gatcagcagc acgttgagca cgaacagctc ggaggatttc
     1261 tgccgcgcca ccaggtgaaa ccagccgcgc atcagccgct ggccgaggaa cagcatcagc
     1321 gacaggacca ccaccgcctt gacgaacgcc aggcccaggt tggaggcgag atcctccgtg
     1381 ccggccgcga gggccggcac caggatcagc agcggcacca ctgccaaatc ctggaacagc
     1441 agcacgccga tgatctggcg tccgtggggc gagttgagct ccaggcgctc cgcgagcatc
     1501 ttggcgacaa tcgccgtgga agacatcgcc agaacaccgc ccagtatcag tcccgcgcgc
     1561 cactcgacgt ccagcgcggc ggcgatgccc cagacgatga gcagggtggc gagcacctgc
     1621 gccgtgccca ggccgaacac gatgcggcgc atggtgaaaa gccggggcag gctgaactcc
     1681 agcccgatgc tgaacatcag gaacaccacg ccgatttcgg ccaggccgcg cacttccgcg
     1741 gtctcggaaa tccatccgag ggcgtggggc ccgatcgcga cgccgacgat caggtagccg
     1801 agcatgggcg gcagccgcaa cgcgcggaac accgcgacga ccagcaccgc ggcggccagc
     1861 aggatcagca cggattgcag cggagattgc ataggtggag caagctttct cgacaacgac
     1921 agttcgaacg gcgcccggga acgggccgca ggaactcccg tcactcgaca ctcgtcacag
     1981 cgtcacgcgt atctcgatga atgatgcctt atcgtccgcg gattgcaagc aaaggaccgg
     2041 gcccgcgggc ggggaaaagt gggtgattct gctatacttt ccgcgcgatg aagacggcat
     2101 ccggcggcgt ccgccgcgca ccccaggaga gcgctgtgct cgccatggcg cgcgatgtgc
     2161 tggagatcga ggcgaaagcc gtttccgccc tgatcggcag gctcgaccag ggcttcgtgc
     2221 aggccgtgga actgctgctg ggctgccgcg ggcgcgtggt ggtgagcggc atcggcaagt
     2281 caggacatat cgcgcgcaag atcgcctcca cgctcgccag caccggcacc ccggcttttt
     2341 acgtccatcc cgcggaggcc agccacggcg atctcgggat ggtcgcgcgc gacgatgtgt
     2401 tcatcgcgct gtccaattcc ggcgagagcg acgaactgct cgccatcgtg ccgctgctga
     2461 agcggcaggg cgccaagctg gtggcgctga ccggcagccc ccaatcctcg ctcgctcgcg
     2521 aggccgatgt ccacctgtat gccggcgcgg agaaggaagc ctgcccgctc aacctcgcgc
     2581 caacggcgag caccaccgcg gcgctcgcgc tgggcgacgc gctggccgtg gctttgatgc
     2641 aggccaaggg cttcacgcgc gacgagttcg cggcctcaca ccccggcggg ccgctgggcc
     2701 gcaagctgct cacccatgtg cgcgacgtga tgcggcgcgg caaggaagcg cccggcatcg
     2761 ggcacgccgc gatggccatg gaaggcgtgc tggagatgtc gcgcggcaga atgggtatga
     2821 ccgcggttct cgacgaccag ggccgcgtca tcggcatcct gaccgacggc gacctgcgcc
     2881 gcgccctgga gaaggggttt gacctgcgct ccgcccgtat cgcggaaatc atgacccgcg
     2941 gtccgcgcac gatcggcgaa gacaagctgg ccgccgaggc ggtggaaatc atggaacgct
     3001 tcaaggtcaa ccagctgctg gtggtcgacc gggaccagaa actgctcggc gcgctgaaca
     3061 tgcacgacct gttccgcgcc aaggtgatct aacaacccta gttacaagtc acaggttgca
     3121 agttacaaga tacaagcatg aatcatcagg cggggtttaa cttgaaactt gtaacttgta
     3181 acttgtaact atttgtgcaa accatctacg accgcgccaa gaatatccgc gtcgccgcgt
     3241 tcgacgtgga cggcgtgctg accgacggcg cgctctatta cgccgatttg ggggaggagc
     3301 tgaagcgctt cagcgtccac gatggccacg gcatgaagat gctgaaggac tgcggtatcc
     3361 agctcgccat catcaccagc cgccgctcgc gctgcgtcga ggagcgcgcg cgcaacctgg
     3421 gcatcgagct gctgtatcag ggcgccacgg acaagctcgt ggcgttccgg gaactgctgg
     3481 gccgctgccg catcgaagcg acggcatgcg cctacgtcgg cgacgatctg gtggatctgc
     3541 cggtgatgaa acgatgcgga ctggcggtga ccgtgccg
//
LOCUS       DBNGSG010000095        11348 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1560368, whole genome shotgun sequence.
ACCESSION   DBNGSG010000095 DBNGSG010000000
VERSION     DBNGSG010000095.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 11348)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11348)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11348
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1560368"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..1466)
                     /locus_tag="AC158Q_02395"
     CDS             complement(78..1466)
                     /locus_tag="AC158Q_02395"
                     /inference="COORDINATES: protein motif:HMM:NF026068.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338647.1"
                     /translation="MSRIWIALLIMMAAAGARGAAPGEEFLAAREAFRTGNAERLEKH
                     AAKLKGHLLEPYVAYWQLSLRIDQAPAEDVRRFFAAYPDSPLTERLRNEWLKALGRRG
                     EWEPFNAELPQLLGDDMEIICYALQSRLIVNADDTLPEARPLWFVARDMPESCTPLFE
                     ALVAGGMLSSDDLWLRVRLALESGQVGLARSVAQWLPAGDRPEAHTLQAIASNPAGYL
                     GKPQAFNLKSRAGREAVMFAVHRLARTSPQLASNHWAKHEERFSPDERAYVWGMLGYL
                     GAMRHERNALAWYERAGDLSDLQLAWKARAALRARNWPEVLAAIDAMTARESSEPSWR
                     YWKARALRAEGRREEAIELLKPLAAEFNFYGQLALEDLGGRIRVPATGYKPGAEAIRA
                     VAGNIGLRRALALYRLNLRAEANREWYWAIRAFDDRQLLAAAELALRNEIYDRAIYTA
                     DRTVALHDFKLR"
     gene            complement(1591..3105)
                     /gene="ilvA"
                     /locus_tag="AC158Q_02400"
     CDS             complement(1591..3105)
                     /gene="ilvA"
                     /locus_tag="AC158Q_02400"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HIR3338648.1"
                     /translation="MKKTDYLERILAARVYDVAIETPLEIAPALSARTANRVLLKRED
                     LQPVFSFKLRGAYNKMAQLPAATLKRGVIAASAGNHAQGVALAAQKLGCRATIVMPVT
                     TPQIKIAAVKARGAAVVLRGDSYDEAYAHAHALGRRRRLAFVHPYNDPDVIAGQGTIA
                     MEILRQHPGDIHAVFVPIGGGGLISGIAAYVKCLRPEIKVIGVEPQDADAMARSLEAG
                     RRVKLAQVGLFADGVAVKEVGKETFRLCRELVDDVIRVDTDAICAAIKDVFEDTRVVL
                     EPAGALAIAGVKAYAARTGVRGRTLVAVASGANMNFDRLRFVAEQAEIGEQREAILAV
                     SIPERPGSFRAFCSLVGARNITEFNYRYADSAEAHVFVGLQVRDRNETRKLVKRLRRR
                     GLKTLDFSDNELAELHIRHTVGGHAPTVDNEILYRFEFPERPGALMKFLTAMSSSWNI
                     SLFHYRNHGADYGRVLVGMQVPPRDKRKFRAFLAKVGYPFNEETRNPAYRLFLG"
     gene            3248..3910
                     /gene="rpiA"
                     /locus_tag="AC158Q_02405"
     CDS             3248..3910
                     /gene="rpiA"
                     /locus_tag="AC158Q_02405"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HIR3338649.1"
                     /translation="MTQDELKQAVAKAAIAHVPDDCVVGVGTGSTANFFIAELGRVKH
                     RIEAAVASSEATAKRLKDLGIRVMELNAVNELPVYVDGADEVTRHLAMVKGGGGALTR
                     EKIVAAVARKFVCIADASKLVDVLGKFPLPVEVIPMARSHVGRELVRLGGHPEWRQNF
                     VTDNGNLILDVHGLTIVNPAELESTLNQITGVVTNGLFARRGADVLLLGGERGVETIE
                     RR"
     gene            4146..5672
                     /gene="ppx"
                     /locus_tag="AC158Q_02410"
     CDS             4146..5672
                     /gene="ppx"
                     /locus_tag="AC158Q_02410"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HIR3338650.1"
                     /translation="MPEFSTLAAVDLGSNSFHCQIGRVVGDQVYPLDALREPVRLGAG
                     LTKDKRIDEDTQERALACLKRFGERLRDFDKHGVRAIGTNTLRVAKNASPFLRKAQAA
                     LGFPIEVVAGREEARLIYLGVAHELPASREKRLVVDIGGGSTEIVIGAGYRPQRLESL
                     FMGCVSYSLRFFENGKITKGSMKQAELAARNELQPIVAKFARGNWQQAVGSSGTVRAL
                     AEVIRQNGFGEEGITSAGLDRLRSHLIKAGDMDKVELAALKPDRVPVFPGGLAILNAV
                     FSELDIDSMAVADSAMRQGVLYDLLGRVHHRDMRDVTVAQFMHRYHVDALQARRVGAL
                     AFNLYRKLTAESGEPDEDVARCIAWASKLHEIGISVAFSGYHKHSAYIIGNADMPGFS
                     RDEQARLGVMVQAHRRSLRKVAKEIDEQDVDWSMVFALRLAALFCRSRSDVALPTLQA
                     RRQGRKFRLVLDPAWLARNPLTVTALHDEIREWDEVGFEFKVPDLEEPEAGGDLALAS
                     "
     gene            complement(5691..7199)
                     /gene="pap"
                     /locus_tag="AC158Q_02415"
     CDS             complement(5691..7199)
                     /gene="pap"
                     /locus_tag="AC158Q_02415"
                     /EC_number="2.7.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043751 - polyphosphate:AMP
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate:AMP phosphotransferase"
                     /protein_id="HIR3338651.1"
                     /translation="MFESAELNHDISKAVYRREEAKLRQALLDAQNDLRQNGRLAVLI
                     LIAGVEGAGKGETVNLLNEWMDPRFIETRAFNLPSDEETERPHMWRFWRALPPRGRIG
                     IFFGAWHTQPIVERVMGRIKAGEYDRRISEIQRIERMLCDEGVLLLKFWFHLSKKQQK
                     KRLKQLAKDPRTSWRVTDLDWMLLDRYDDFAAVCEPFIRRTSTAEAPWMPVPGADPQY
                     RSLAVGRTLLAALRERLDEKPVKRLPDRTPPLLPALDRLNVIRALELDQPMTKDEYGK
                     EFEKWQGRLNRLSRHPRFKKVSVVAVFEGNDAAGKGGAIRRVTRSFDARSYHTVSIAA
                     PSEEERAQPYLWRFWRHVPRRGVMAIFDRSWYGRVLVERVEGFCPEADWMRGYGEINE
                     FEQELVRHDVIVVKFWLAISKDEQHRRFKEREKVGYKRFKITEEDWRNRRKWDEYERA
                     VCDMVDRTSTSVAPWTLVEANNKYYARIKVLKTLCGAIEDALKRVKKKKKGR"
     gene            complement(7220..7672)
                     /locus_tag="AC158Q_02420"
     CDS             complement(7220..7672)
                     /locus_tag="AC158Q_02420"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SixA phosphatase family protein"
                     /protein_id="HIR3338652.1"
                     /translation="MELILWRHADADNAAPGKPDAERALTAKGEKQARRVAVWLRKRL
                     PDDARILVSPARRAQQTARALTRHFETNLEVGTTAGPQSILRVAGWPAGDGAVVVVGH
                     QPTLGQAAALALTGKALEWSVKKGALWWIASRGDGDAIVRAVIAPDLV"
     gene            complement(7731..8645)
                     /locus_tag="AC158Q_02425"
     CDS             complement(7731..8645)
                     /locus_tag="AC158Q_02425"
                     /inference="COORDINATES: protein motif:HMM:NF017085.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HIR3338653.1"
                     /translation="MKRSSPGSGKPVKAGPVPLRKGMPVSGAFHAVMLAGLAHLQANE
                     RGVMTGRGPEHLHQMRVALRRLRSAVEIFGPLLPARVVGRVERDARWLGASLNPARDW
                     DVFVMETLPAFERSRWGRGVAGGIAARCEGPRRRAQDAARRAVRSRRYRSFRRSLPAL
                     IAARDRPIRLDDVAAHAVLRGPVEDFASAVLGRRDGQVNKRGRGLGELNARKLHRLRI
                     AIKKLRYAAVFFAGLYGTKAARDSLKRLARLQDILGRMNDAAMTGRLMAHDSAKDNLQ
                     GWSRARTAALRHELKRAWKQYRSAEKFW"
     gene            complement(8694..10775)
                     /gene="ppk1"
                     /locus_tag="AC158Q_02430"
     CDS             complement(8694..10775)
                     /gene="ppk1"
                     /locus_tag="AC158Q_02430"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009668144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HIR3338654.1"
                     /translation="MNKPRDIPELYLNREIGQLAFNRRVLAQAENRATPLLERLRFLC
                     IVSSNLDEFFEIRVSGLHAEIEAGSPVIGPDRMRADDLFKRVAQEVHQLVAAQYQQLN
                     QEILPGLAAEGVRFLRRGDFTSAQSEWIKAYFFREVMPVLTPIGLDPSHPFPRVLNKS
                     LNFAVELEGKDAYGRNSGIAIVQAPRVLPRVIRLPREISEAEYDFVFLSSVMHAHVSE
                     LFAGMKVVDSYQFRVTRNSELFVDEEEVKDLRTALRGELPHRHFGDEVRLEVADNCPQ
                     QISDYLLAQFNLGPEDLYRADGPVNLVRLMNVPDGVNRPDLKYPVFRPGLPAQLQRGG
                     DLFDSIREADILLHHPFQSFQPVIAFLEQAARDPAVVAIKMTVYRTGAESELMDILIT
                     AARAGKEVTVVLELLARFDEEANINWAQRLEEVGAHVVYGVVGHKTHAKMLMVVRRED
                     ERLKRYVHLATGNYHPRTTKLYTDFGLFTASEEIGTDVSDIFIQLTGLGRASKLKHLW
                     QAPFTLHKQILRAIQNEIQLAQGGAKGRIIAKMNALLEPETIAALYEASKAGVEIDLI
                     VRGVCALKPGIPGVSENIRVCSVVGRFLEHSRVFYFGNGGAEDVYLSSADWMDRNFFR
                     RVEICFPVLDPKLKKRVINEGLKPYLDDNCQAWDMDAEGLYSLRQPRRGKPQAAQENL
                     LDLLARAQLPA"
     gene            complement(10860..11135)
                     /locus_tag="AC158Q_02435"
     CDS             complement(10860..11135)
                     /locus_tag="AC158Q_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HIR3338655.1"
                     /translation="MPRMVNCVKLGREAEGLDFPPYPGGLGKRIFDSVSKEAWKQWLE
                     QQKMLVNENRLNLADRKARDYLAQQMEKHFFGGGADTASGYVPPPGK"
BASE COUNT         1830 a         3840 c         3727 g         1951 t
ORIGIN      
        1 atcagcccgt agacccacgc ctcgtccagt tccagctgcg cggtatgggc ggtgagcacc
       61 tcgcgatagg gtgccagtta gcgcagcttg aaatcgtgca gggccacggt gcggtcggcg
      121 gtatagatgg cgcggtcgta gatctcgttg cgcagcgcga gttccgcggc ggccagcagc
      181 tgccggtcgt cgaacgcgcg aatcgcccag taccattccc ggttcgcctc cgcgcgcagg
      241 ttaaggcggt agagcgcgag cgcgcgccgc aggccgatat tcccggccac ggcgcgtatc
      301 gcttccgcgc ccggcttgta gccggtggcg ggcacccgga tcctgccgcc cagatcctcc
      361 agcgcgagct ggccgtagaa gttgaattcg gcggcgagcg gcttcaacag ctctatcgct
      421 tcctcgcggc gtccctcggc acgcaacgcc cgcgccttcc agtagcgcca cgagggctcc
      481 gaggactccc tggcggtcat cgcgtcaatg gccgccagca cctccggcca gttgcgggcg
      541 cgcagcgcag cgcgcgcctt ccaggcgagc tgcaggtccg aaagatcgcc ggcgcgctcg
      601 taccaggcga gtgcgttgcg ctcgtgccgc atcgcgccca ggtagccgag catcccccag
      661 acgtaggcac gctcgtccgg ggaaaaccgc tcttcgtgct tcgcccagtg attcgacgcg
      721 agctggggcg aggtgcgcgc gagccgatgg acggcgaaca tgacggcttc gcgcccggcc
      781 cggctcttga gattgaacgc ctgcggcttg ccgagatacc ccgccgggtt cgacgcaatg
      841 gcctggagcg tgtgcgcctc gggcctgtcg cccgcgggca gccactgtgc gacgctgcgc
      901 gcgagtccga cctggcccga ctccagcgca agccgcacgc gcagccacag gtcgtcgctg
      961 gacagcatgc cccccgcaac gagcgcttcg aacaggggcg tgcagctctc cggcatgtcg
     1021 cgcgcgacga accacagcgg ccgggcttcg ggcagcgtgt cgtccgcgtt gacgatcagt
     1081 cgcgactgca gcgcgtagca gatgatctcc atgtcgtcgc cgaggagctg cggcagctcg
     1141 gcgttgaacg gctcccattc cccgcgccgg ccgagcgcct tgagccattc attgcgcagc
     1201 cgctcggtga gcgggctgtc cgggtaggcg gcgaaaaatc tgcgcacgtc ctcggccggg
     1261 gcctggtcga ttctcaggct caactgccag taggccacgt acggctcgag cagatgaccc
     1321 tttagtttcg cggcgtgctt ttcgagccgc tcggcgttgc cggtgcggaa agcctcgcgc
     1381 gcggcaagga actcttcgcc gggcgcggcg ccccgcgcgc cggccgcggc catcatgatc
     1441 agcaacgcaa tccagatcct gctcatatcc gacacgaaat cccctgcgta gccgcctaat
     1501 tctagccgtt ttttttattc cccagcccat tagacaacgc gccatgagaa ttggcgttga
     1561 cgcaactcgg ccgggaagcc ggcgcgccga tcagcccagg aacagccgat aggcgggatt
     1621 gcgggtttcc tcgttaaagg gatagccgac cttggcgaga aaagcgcgga acttgcgctt
     1681 gtcgcgcggc ggcacctgca tgccgaccag cacgcgcccg tagtcggcgc cgtggttgcg
     1741 atagtggaac aggctgatgt tccagctgct gctcatggcg gtgaggaact tcatcagcgc
     1801 gccggggcgc tcgggaaact cgaagcggta gagtatttcg ttgtccacgg tcggggcatg
     1861 gccgcccacg gtgtgccgga tatgcagctc ggccagttcg ttgtcgctga aatcgagggt
     1921 cttcagcccg cgccggcgca gcctcttcac cagtttgcgg gtttcgttgc ggtcgcgcac
     1981 ctgcaggccg acgaacacgt gggcctcggc cgagtcggca tagcggtagt tgaactcggt
     2041 gatgttgcgc gcgccgacga gcgagcaaaa agcacggaag ctgcccggcc gctccgggat
     2101 gctcacggcc aggatcgcct cgcgctgctc gccgatctcg gcctgctcgg cgacgaagcg
     2161 caggcgatcg aaattcatgt tggcgccgct ggcgaccgcc accagcgtcc tgccgcgaac
     2221 gccggtgcgc gcggcataag ccttgacgcc ggcgatcgcc agcgccccgg ccggttccag
     2281 gacgacgcgc gtgtcctcga acacgtcctt gatcgcggcg cagatggcat cggtatcgac
     2341 ccggatcacg tcgtccacca gctcgcggca caggcggaaa gtctctttac cgacttcctt
     2401 caccgccacg ccgtcggcga acaaccccac ttgcgccagt ttcacgcgcc gcccggcctc
     2461 gagcgagcga gccattgcgt cggcgtcctg cggctccacg ccgatcacct tgatctcggg
     2521 acgcaggcac ttcacgtacg cggcaatgcc cgagatcaac ccgccgccgc cgatggggac
     2581 gaagacggca tggatgtccc cgggatgctg gcgcaggatc tccatcgcga tcgtgccttg
     2641 cccggcgatc acgtcggggt cgttgtaggg atgcacgaaa gcgagacggc ggcgccgtcc
     2701 cagcgcgtgc gcgtgagcgt aggcctcgtc ataagagtcc ccgcgcagca cgacggccgc
     2761 gccgcgcgcc ttgaccgccg cgatcttgat ctgcggcgtc gtgaccggca tcacgatggt
     2821 ggcgcggcag ccgagcttct gcgccgcgag cgcgacgccc tgcgcgtggt tgccggcgga
     2881 agcggcgatc acgccgcgct tgagggtggc ggccggcagc tgcgccatct tgttgtaggc
     2941 cccgcgcagc ttgaaggaaa acaccggctg caggtcctcg cgcttcaaca gcacgcggtt
     3001 ggcggtgcgc gcggagagcg cgggcgcgat ctccagcggc gtctcgatgg cgacatcgta
     3061 gacgcgcgcg gcaagaatgc gctcgaggta gtcggtcttt ttcatggcgg cggcaaacgg
     3121 agtcaagcgt agcaggaatg gccgcggaat ggaaaatcgt gattcgtgaa gggtgattcg
     3181 tgattggtgt atagtggttt gcgtcctccg aatcacgaat cacccttcac caatcacgct
     3241 gccggcgatg acgcaagacg aactgaagca ggccgtggcg aaagccgcga tcgcccacgt
     3301 ccccgacgat tgcgtggtag gcgtcggcac cggatccacc gcgaatttct tcatcgccga
     3361 gctgggccgg gtgaaacacc gcatcgaggc cgcggtggcg agctccgaag cgacggcaaa
     3421 gcggctgaaa gacctcggca tccgcgtgat ggagctcaat gcggtgaacg agctgccggt
     3481 gtacgtggac ggcgcggacg aggtcacgcg ccacctcgcc atggtgaagg gcggcggcgg
     3541 cgcgctgacg cgggagaaga tcgtggcggc ggtcgccagg aagttcgtgt gcatcgccga
     3601 tgcgtccaag ctcgtggacg tgctcggaaa atttcccctg ccggtcgaag tgatcccgat
     3661 ggcgcgctcc cacgtcgggc gcgagctcgt ccggctcggc ggccacccgg agtggcggca
     3721 gaacttcgtc accgacaacg gcaacctgat tctcgacgtg cacgggctca cgatcgtcaa
     3781 tcccgccgag ctggaaagca cgctcaacca gatcaccggc gtcgtcacca atggactgtt
     3841 cgcgcggcgc ggcgccgacg tgctgctgct cggcggcgag cgcggcgtcg agaccatcga
     3901 gcgccggtaa ttttcgcggc gcgctgcgat cgcgcaacat ccgcttcata ttctcccgcg
     3961 cagctggcgg cgcgccgcaa gattccttat actggcgtcc agagtcgtcg agaggacgcc
     4021 atcacgcaac cgcgcccgtt caacccgttg caataccgcc gcgccctcgc cgccgccgcg
     4081 gcgtctctcc tcttccgcgc gtcgcgccac gtccgcgacc gccgcctcat cccctggtaa
     4141 gcaccatgcc cgagttctcc accctggctg cggtcgatct cggttcgaac agcttccatt
     4201 gccagatcgg gcgcgtcgtc ggagaccagg tctaccccct cgatgcgctg cgcgagccgg
     4261 tgcggctggg ggcgggactg accaaggaca agcgcatcga cgaggacacc caggagcgcg
     4321 cgctcgcctg cctgaagcgc ttcggtgagc ggctgcgcga cttcgacaag cacggcgtgc
     4381 gcgcgatcgg caccaatacg ctgcgggtgg caaagaacgc ttcgcctttc ctgcgcaaag
     4441 cgcaggcggc gctcggcttc ccgatcgagg tggtggcggg ccgcgaggaa gcccgcctga
     4501 tctatctcgg cgtcgcgcat gaactgcccg cctcacgcga gaaacggctg gtggtggaca
     4561 tcggcggggg ctccaccgaa atcgtcatcg gcgcgggcta ccggccgcag cggctggaga
     4621 gcctgttcat gggttgcgtg tcctacagcc tgcgcttctt cgagaacggc aagatcacga
     4681 agggctcgat gaagcaggcc gagctcgccg cccgcaacga gctgcagccg atcgtggcga
     4741 aattcgcgcg cggcaactgg cagcaggcgg tgggatcctc gggcaccgtg cgcgccctgg
     4801 cggaagtcat caggcagaac ggcttcggcg aggaaggtat cacgtcggca gggctggacc
     4861 gcctgcgctc ccatctcatc aaggcggggg acatggacaa ggtggaactg gcggcgctga
     4921 agcccgaccg cgtgccggtg ttccccggtg gtctggcgat cctgaacgcg gtattctccg
     4981 agctcgacat cgattcgatg gcggttgccg acagcgcgat gcgccagggc gtgctctacg
     5041 acctgctggg gcgcgtccac cacagggaca tgcgcgacgt cacggtcgcg cagttcatgc
     5101 accgctacca cgtggacgcc ctgcaggcgc ggcgcgtggg cgcgctcgcc ttcaacctct
     5161 accgcaagct gaccgccgag tcaggcgagc ccgacgagga tgtcgcgcgc tgcatcgcat
     5221 gggcatcgaa gctgcacgag atcggcattt cggtcgcatt cagcggctat cacaagcatt
     5281 ccgcctacat catcggcaac gccgacatgc cggggttctc ccgcgacgag caggcccggc
     5341 tcggagtgat ggtccaggcg caccgccgct cgctcaggaa ggtggcgaaa gagatcgacg
     5401 agcaagacgt ggactggagc atggtcttcg cgctgcggct ggcggcgctg ttctgccgca
     5461 gccgctcgga cgtggcgctg cccacgctgc aggcaaggcg ccagggcagg aaattccgcc
     5521 tggtgctcga cccggcatgg ctcgcccgta atccgctgac ggtgaccgcc ttgcacgacg
     5581 agatccgcga gtgggacgaa gtcggcttcg aattcaaggt ccccgacctc gaggagccgg
     5641 aagcgggcgg cgatctcgcc ctggcgagct aggcgcggcg cgcggcgcct ctacctgccc
     5701 ttctttttct tcttgacgcg cttcagcgcg tcctcgatcg cgccgcacag ggttttcagc
     5761 accttgatgc gggcatagta cttgttgttg gcctcgacca gggtccacgg cgcaacggaa
     5821 gtgctggtgc ggtccaccat gtcgcagacc gcgcgctcgt actcgtccca tttcctgcgg
     5881 ttgcgccagt cctcctcggt aatcttgaag cgcttgtagc cgaccttctc gcgctccttg
     5941 aagcgccggt gctgctcgtc cttgctgatc gccagccaga acttgaccac tatgacatcg
     6001 tgccgcacaa gctcctgttc gaactcgttg atctcgccgt agccgcgcat ccagtcggct
     6061 tccgggcaga agccttccac gcgctcgacc agcacgcggc cataccacga ccggtcgaag
     6121 atggccatga cgccgcgccg cggcacgtgc cgccagaacc gccacaggta cggctgcgcg
     6181 cgctcttcct cgctcggcgc cgcgatcgac acggtatggt agctccgcgc gtcgaacgag
     6241 cgcgtgacgc ggcgaatggc gccgcccttg cctgcggcgt cgttgccttc gaacaccgcc
     6301 accaccgaga ccttcttgaa gcgcgggtgc cgtgacagcc ggttcaaccg accctgccat
     6361 ttttcgaact ctttcccgta ttcgtccttg gtcatcggct ggtcgagctc gagcgccctg
     6421 atgacgttga gccggtcgag ggcgggcagc agcggcgggg tcctgtccgg caggcgcttg
     6481 accggtttct cgtcgagacg ctcgcgcagc gcggccagca gcgtccggcc cacggccagc
     6541 gaccggtatt gcggatcggc gcccggcacc ggcatccacg gcgcttccgc ggtgctggtc
     6601 ctgcgtatga agggctcgca caccgcggcg aagtcgtcgt agcgatccag cagcatccag
     6661 tccaggtcgg tcacgcgcca gctggtcctg ggatctttcg cgagctgttt caggcgcttc
     6721 ttctgctgct tcttcgagag gtggaaccag aacttgagca gcagcacgcc ctcgtcgcaa
     6781 agcattcttt cgatgcgctg gatctcggag atgcggcggt cgtactcgcc cgccttgatg
     6841 cgccccatga cgcgctcgac gatgggctgg gtatgccagg cgccgaagaa gatgccgatc
     6901 cggccccgcg gcggcagcgc ccgccagaag cgccacatgt gggggcgctc ggtttcctcg
     6961 tccgagggca agttgaatgc gcgcgtctcg atgaagcgcg ggtccatcca ttcgttcagc
     7021 aggttgaccg tctcgccctt gcccgcgcct tccacgcccg cgatcaggat cagcacggcg
     7081 aggcgcccgt tctgccgcag gtcgttctgc gcgtcgagca gcgcctggcg cagtttcgcc
     7141 tcctcgcggc ggtagaccgc cttgctgatg tcgtgattga gttcagccga ttcgaacatg
     7201 agccctcccg ggacgccctc taaaccaggt ccggcgcgat caccgcgcgc acgatcgcgt
     7261 caccgtcgcc gcggctcgcg atccaccaca gcgccccttt tttcacgctc cattccagcg
     7321 ctttgccggt gagggccagc gcggccgcct gccccagggt cggctggtgg ccgaccacca
     7381 cgacggcgcc gtcaccggcc ggccagcccg ccaccctcag gatggactgc ggcccggccg
     7441 tggtgccgac ctcgaggttg gtctcgaagt gccgggtgag cgcccgggcc gtctgctgcg
     7501 cgcgcctcgc cgggctcacc aggatgcgcg catcgtcggg cagccgcttt ctgagccaga
     7561 ccgcgacacg ccgcgcctgc ttctcgcctt tggcggtcag ggcgcgttcg gcatcgggct
     7621 tgccgggtgc cgcgttgtcg gcgtcagcgt ggcgccacag gattaattcc ataaggcgtg
     7681 attcgtgggt cgtgggtcgt aattcgcagg caacgaagat ttccggtggt tcaccagaat
     7741 ttttcggcgg agcggtattg cttccacgcg cgcttcagtt catgcctgag tgccgcggtc
     7801 cgggcgcggc tccaaccttg caggttgtcc ttcgcgctgt catgcgccat gagccggccg
     7861 gtcatcgccg cgtcgttcat tcttcccagt atatcctgca gccgcgcaag ccgtttcagg
     7921 gaatcgcgcg ccgctttcgt cccgtacagg ccggcgaaaa atacggcggc atagcggagc
     7981 ttcttgatcg cgatccgcaa ccggtggagc ttgcgggcgt tcagttcgcc cagcccgcgg
     8041 cccctcttgt tcacctggcc atcgcgccgc cccaaaaccg cgctggcgaa atcctccacc
     8101 gggccgcgca ggacggcatg cgccgcaaca tcgtcgagcc ggatcggccg atcccgcgcc
     8161 gcgatcaggg ccggaagcga acgcctgaag ctccggtaac gccgcgaacg aacggcccgc
     8221 cgcgcggcgt cctgggcgcg gcggcgcggc ccttcgcaac gcgcggcgat accgcccgca
     8281 acgccgcgcc cccagcgtga ccgctcgaac gccggcagcg tctccatcac gaacacgtcc
     8341 cagtcgcgcg ccgggttgag gctcgcgcca agccatctgg catcgcgttc cacccggcca
     8401 accacccgcg cgggcaacag gggtccgaag atttcgaccg cggaacgcaa ccgccgcaac
     8461 gcgacccgca tctggtgcag gtgctcgggg ccgcgcccgg tcatcacccc gcgctcgttg
     8521 gcttgcagat gcgcgagacc ggccagcatc acggcatgga atgcgccgct gaccggcatg
     8581 ccctttcgga gcgggaccgg cccggctttc accggcttgc cggaaccggg gctggaacgt
     8641 ttcatgagga ctccgcgaac cggcgcggcg gtgaaccggc tccgtctgcg cacttatgcc
     8701 ggcagctggg cgcgggcgag cagatcgagc aggttctcct gcgccgcctg gggcttgccg
     8761 cggcgcggct ggcgcaggct gtagaggcct tccgcgtcca tgtcccaggc ctggcagttg
     8821 tcatcgagat agggcttcag tccctcgttg atgacgcgct tcttcaattt gggatcgagc
     8881 accgggaagc agatttccac gcgccggaaa aaattgcggt ccatccagtc ggcgctggaa
     8941 aggtacacgt cctcggcgcc gccgttgccg aaatagaaca cgcgcgagtg ctcgaggaag
     9001 cgtccgacca ccgagcacac gcggatgttc tccgacacgc cgggtatgcc gggcttcagc
     9061 gcgcacaccc cgcgcacgat gaggtcgatt tcgacgccgg ccttggaggc ctcgtagagc
     9121 gcggcgatag tctcgggctc gagcaatgcg ttcatcttgg cgatgatgcg gcccttcgcc
     9181 ccgccctggg caagctggat ctcgttctgg atcgcgcgca ggatctgctt gtgcagggtg
     9241 aacggcgcct gccacaggtg cttcagcttc gatgcgcgcc cgaggccggt caactggatg
     9301 aagatgtcgg agacgtcggt cccgatctcc tcgctggcgg tgaagaggcc gaaatccgtg
     9361 tagagcttgg tggtgcgcgg gtggtaattg ccggtggcaa ggtgcacgta gcgcttcagg
     9421 cgctcgtctt cgcgccgcac caccatcagc atcttggcgt gggtcttgtg cccgaccacg
     9481 ccgtagacca cgtgcgcgcc gacttcctcc aggcgctgcg cccagttgat gttggcctcc
     9541 tcgtcgaagc gcgcgagcag ttccagcacc accgtcactt ccttgccggc ccgcgccgcg
     9601 gtgatcagga tatccatcag ctccgattcg gccccggtgc ggtagaccgt catcttgatc
     9661 gcgaccaccg cgggatcgcg cgccgcctgc tcgagaaacg cgatcaccgg ctggaacgac
     9721 tggaacggat ggtgcagcag gatgtccgcc tcgcggatcg agtcaaacag gtcccccccc
     9781 cgctggagct gcgccggcag cccggggcgg aacacggggt acttgagatc cggccggttg
     9841 acgccatccg gcacgttcat cagccgcacc aggttcaccg gcccgtccgc gcggtagagg
     9901 tcttccgggc cgaggttgaa ctgcgcgagc aggtaatcgg agatctgctg cgggcagttg
     9961 tcggcgacct cgagccggac ctcgtcgccg aagtggcggt ggggcagctc gccgcgcagc
    10021 gcggtgcgca ggtccttcac ttcttcctcg tccacgaaca gctcgctgtt tctcgtcacc
    10081 cggaactggt agctgtccac caccttcatg ccggcgaaca gctccgacac gtgcgcgtgc
    10141 atcaccgagg acaggaacac gaaatcgtat tcggcctccg agatttcccg gggcaggcgg
    10201 atcacccgcg gcagcacccg cggcgcctgc acgatggcga taccggaatt gcgcccgtac
    10261 gcatccttgc cttcgagctc gacggcgaag ttcaagctct tgttcaatac gcgcgggaac
    10321 ggatgcgacg ggtcgagccc gatcggcgtc aataccggca tcacctcgcg gaaaaaataa
    10381 gccttgatcc attccgattg cgcgctggtg aaatcgcccc ggcgcaggaa gcgcacgccc
    10441 tcggccgcga gcccgggcag gatttcctgg ttgagctgct ggtactgcgc cgccaccagc
    10501 tggtgcacct cctgcgccac ccgcttgaag agatcgtcgg cgcgcatgcg gtccgggccg
    10561 atgaccgggg acccggcctc gatctcggca tgcaggccgg agacgcggat ctcgaagaat
    10621 tcgtcgagat tggaggagac gatgcacagg aaccgcagcc gctcgagcag cggggtcgcc
    10681 cggttttccg cctgggcgag cacgcgccgg ttgaacgcga gctgcccgat ctcgcggttc
    10741 aggtacagct ccgggatgtc gcgcggcttg ttcatcgcgt cgcgccggcg cggattgcct
    10801 gtgccggtcg cggcacaggc atcggtccgc ggctcggtca gtctggttgt agggcgtgcc
    10861 tatttgccgg gaggtggaac gtaaccggag gcggtatccg cgccgccgcc gaagaaatgc
    10921 ttctccatct gctgcgcgag gtaatcgcgc gccttcctgt ccgcgaggtt gagccggttt
    10981 tcattgacca gcattttctg ttgttccagc cactgcttcc aggcttcctt cgatacgctg
    11041 tcgaaaatcc gcttgccgag cccgcccgga tagggcggaa aatcaaggcc ttccgcctcg
    11101 cggccaagct tcacgcagtt caccattcta ggcatggatc cgccttcaag ccagtggcaa
    11161 tgtatgacat caatattgcg gaaatgttaa gagtccctaa cacaatgaaa cgcgcgatgc
    11221 gctgtcacga ttttggctca gggcgcgaag cgagccgcag ccaatatgtc gatattagca
    11281 aggcgagcga caaagccatg ggccaaaact cgtgccagcc cccatgtttc ccttggatat
    11341 tccgatgc
//
LOCUS       DBNGSG010000096         9669 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1051111, whole genome shotgun sequence.
ACCESSION   DBNGSG010000096 DBNGSG010000000
VERSION     DBNGSG010000096.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9669)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9669)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9669
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1051111"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3008
                     /gene="dnaE"
                     /locus_tag="AC158Q_02440"
     CDS             <1..3008
                     /gene="dnaE"
                     /locus_tag="AC158Q_02440"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR3338656.1"
                     /translation="GRNATVHGAASVPLETYVRRALGLASALKLPVVATHPVQFVKRE
                     DFRAHEARVCIAQGYTLSDQRRPRQHSAEQYFRTQAEMAQAFRDVPQALANSVEIAKR
                     CNLDIELGKTRLPRFQTPGDMSLDDYLRSRAAEGLEHRLPQLYPDAGQRAQQVTRYRE
                     RLDFEIRTIQQMGFSGYFLIVADFINWAKGNGVPVGPGRGSGAGSLVAYSLGITDLDP
                     LRYALLFERFLNPERVSMPDFDIDFCQDGRDRVIEYVKSKYGADSVSQIVTFGTMAAR
                     AVVRDVGRVLDLSYTFCDQLAKLIPFQPGRYITLEDARKEEPQFREREQKEEEVRELV
                     ALAEQLEGLTRNVGMHAGGVLIAPGKLTDFCPLYTPDGSDSVVSQFDMKDVEAVGLVK
                     FDFLGLTTLTILDWTLRFVRQLDPASKVKLEETPLDDPASYRVFAQADTTGVFQFESR
                     GMRDLLARAKPDRFEDIVALVALYRPGPMELIPEFIKRKHGGRVDYPDPRVEPILKPT
                     YGIMVYQEQVMQIAQIIGGYSLGAADLLRRAMGKKLPEEMAQHRSSFVAGAAKNDVGA
                     RRAHELFDLMEKFAGYGFNKSHAAAYALVAYQTAWFKAHHASAFLAANMSAVMNDTDK
                     VKQFVDDTQAHGIVLRAPDINESNYRFEPVDAKTIRYGLGGIKGTGEAAINSILSARK
                     EGGAFRDLFDFCHRVDKRLVNRRVVESLIRAGAFDSINDHRASLLASVGMALESAEQA
                     SRSVNQVSLFGDLTDSTQQPALAGVPRWGAKERLQNEKLALGFYLSGHLFNIYRDEVR
                     GFARTRIGELAALSPGEYAGRTYWVAGVVMGMRIQNTASGRMGVITLADDSDSNEVIF
                     FGEAFDKFRQKIREDELLVVEVQLRARGGGGRMVNGEYEGGGEARASIRAVNALDFNE
                     ARNRFARGVRITCNGASSGGRLRDLLAPYRSGRCPVSVVYSNRGAVCEIDLGESWRVN
                     LHDDLIHSLSEWLSPENVKIVYNESRADQ"
     gene            3146..3823
                     /locus_tag="AC158Q_02445"
     CDS             3146..3823
                     /locus_tag="AC158Q_02445"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3338657.1"
                     /translation="MLSLLLAASPAAAGEAEWKMFYEQSAAHLQQGSLEQAELFAREA
                     LRESELLPGKSRATELSLLRAAHVLRLRGKADEALPLAERAVKISAQLHGANSPDTAV
                     ALQIQAEVQSARRQYSAAEALHRKALSVFQKTYGDRHLSVAVSLHNVGAMLYAQDKNK
                     EAEKYLRRALVVKEQVLKPGNLSIAHTLDQLSIVLAVQGREIEAERYRKRAEGIRRRV
                     QAPKPSA"
     gene            complement(3921..4199)
                     /locus_tag="AC158Q_02450"
     CDS             complement(3921..4199)
                     /locus_tag="AC158Q_02450"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HIR3338658.1"
                     /translation="MERVTRHLIITGRVQGVGYRFYMQRKARELGVAGWVRNRLDGSV
                     EATVQGDMSAVEAMIAWVRRGPPSAVVAEVQVEVGTGEFSGFETLPTE"
     gene            4658..6670
                     /gene="hyfB"
                     /locus_tag="AC158Q_02455"
     CDS             4658..6670
                     /gene="hyfB"
                     /locus_tag="AC158Q_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HIR3338659.1"
                     /translation="MNSVFFQPIEAAVTLSVIWIGLGVIGLAFTRAPRMITHAVFPAG
                     ALVSLLLAATGLWAIGAAPSTAILPAGLPDLPFHVRIDALSGFFLLLLGGVSFGISLF
                     AVGYFRATGGTALGLLNLQYHVFLASMAFVLIADDAYLFMITWESMALSSYFLVTTEH
                     HEEQNRKAGFIYLLVAHIGAISILLCFGVLHGGHGDYTFDALRAANLGGSWATVAFLL
                     AFFGFGAKAGMLPLHVWLPEAHPAAPSPVSALMSGVMLKTAIYGMVRVIYDLIGGIRW
                     EWGLTVLLFGAATSLFGVLFALMQHDLKRLLAYHSVENIGIILIGIGLSMVFIGSGHP
                     VAGALGLIAGLYHTLNHAVFKGLLFLGAGSILKSTGLRNLNEMGGLIRHLPHTALYFL
                     IGALAISALPPLNGFVSEWLTFQASLQAPLLDSSVVRSLVPIVAAVLALSGALTGMCF
                     VKVYGVAFLGQQRHDFVEAPREASGWERAGMLWLALACFALGLFPVFMIGELNKVSLA
                     LISTGLNEEAMAAGWLWLAPTSSAQASYSPVVFLLVITAVLGLTFMAVRAIYHGRLRR
                     VDPWDCGFPAQTPRMQDSADAFGQPIRQIFEPVYRIRREIPRADDTHPVFTQSVEDRH
                     WYAMYLPIARFTEFLSGHVGRLQQGRISVYLLYSFLTLIALLVFAQ"
     gene            6667..7617
                     /locus_tag="AC158Q_02460"
     CDS             6667..7617
                     /locus_tag="AC158Q_02460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010810545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory chain complex I subunit 1 family
                     protein"
                     /protein_id="HIR3338660.1"
                     /translation="MNITGAIVFQVLQSVLVVLIAPLMLGWVNQCRAWLQNRSGAGIW
                     QPYKVLHKLFHKDAVLAHNASPLFRVTPYILFGCMWLAVGIVPVLATSLPFAPAADVI
                     ALVGLFALARVFISLAGMDVGTSFGTLGARREMMIGFLAEPAMLMTLFTASLISQSTS
                     LATIVETLGHRELVIYPSMAFAAVAFLMVLLAENARVPIDNPSTHLELTMIHEAMILE
                     YSARHLALIEWAASLKLTAYVTIGIALFVPWGIAGPGDWQALPLALAALTLKLVAAGA
                     GLALLETVSAKMRIFRAPEFLGTAFMLATLGMLIHFLLER"
     gene            7625..8287
                     /locus_tag="AC158Q_02465"
     CDS             7625..8287
                     /locus_tag="AC158Q_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase"
                     /protein_id="HIR3338661.1"
                     /translation="MNLPLHAQLINLFAALMLLLAFAMLTQRRILSLINLFAAQGFVL
                     VLSTLVVAYFTNQGHLYYSALLTLALKVLLLPWIMHRLIRALNVQWDVETLINIPATM
                     LIGIVLVVFAFNLADPISQFAGTITKSTLGIATACILLAFLMMITRRKAVSQVIGFLA
                     MENGLFFAATSATYGMPMVVELGIALDVLVATLIFGVFFFHIRETFESLDIHHLEKLK
                     ER"
     gene            8287..>9669
                     /locus_tag="AC158Q_02470"
     CDS             8287..>9669
                     /locus_tag="AC158Q_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HIR3338662.1"
                     /translation="MELLLLLGAPFAGGILLGFWGHRNFAPELNSGMSLLTFLAAAAL
                     TVRIIADGPILVWDEMFFVDSFNVFLVALTAFVAFTTSLFSRPYMRIELALGKLSPNR
                     LRLYHSMYQMFTFTMLLVLLTNNIGILWVAMEAATLTTVLLVSLYRTPASLEAAWKYF
                     ILCSVGLAQALFGTILLYFAAEKVLGAGGNALLWTHLYGVKDQLEPTVLGIAFVFLLV
                     GYGTKVGLAPLHNWLPDAHAEGPTPVSAVLSGLLLNVALYAVVRTKVLVEGALGTHFA
                     GYLMMGFGLLSVVVAAFLLSRQKDAKRLFAYSSIEHMGLMTFAFGMGGALATFAGLLH
                     MTVHSLTKSAIFFTVGHAAQKSGTQVMANIRGLIDSNPTIGWGLMLGSLAILGIPPFG
                     VFASEFMILTVTMHEQPWATPFLLVALGVAFAAIFGKVQPMVFGTATAERLPHPPALV
                     PVFVHLAIVLL"
BASE COUNT         1552 a         3123 c         3158 g         1836 t
ORIGIN      
        1 tgggcaggaa cgcgacggtg cacggcgcgg cgagcgtccc gctggagacc tacgtccggc
       61 gcgcgctggg gctcgcctcg gcgttgaagc tgccggtcgt ggcgacgcac ccggtgcagt
      121 tcgtgaagcg cgaggacttc cgggcgcacg aggcgcgcgt ctgcatcgcc cagggctaca
      181 cgctgtcgga ccagcggcgg ccgcgccagc acagcgcgga gcagtacttc cgcacccagg
      241 ccgagatggc gcaggcgttt cgcgacgtgc cgcaggcgct cgccaacagc gtggagatcg
      301 ccaagcgctg caacctggac atcgagctgg gcaagacgcg cctgccgcgg ttccagacgc
      361 cgggggacat gagcctcgac gactacctgc gcagccgcgc ggcggagggg ctggagcacc
      421 ggctgccgca gctctatccc gacgcgggcc agcgcgcgca acaggtgacc cgctatcgcg
      481 agcggctcga tttcgagatc cgcaccatcc agcagatggg gttttccggt tatttcctga
      541 tcgtggccga tttcatcaac tgggcgaagg gcaacggggt cccggtgggg ccgggacgcg
      601 gctccggggc gggatcgctc gtggcgtatt cgctcggcat caccgacctc gacccgctgc
      661 gctacgccct gctgttcgag cgctttctca accccgagcg cgtgtccatg ccggatttcg
      721 acatcgactt ctgccaggac ggtcgcgacc gggtgatcga atacgtgaag agcaagtacg
      781 gcgccgacag cgtttcccag atcgtcacct tcggcaccat ggcggcgcgc gcggtggtgc
      841 gcgacgtcgg gcgcgtgctc gatctctcgt acacgttctg cgaccagctc gccaagctca
      901 tcccgttcca gccgggccgc tacatcacgc tcgaggacgc gcgcaaggag gagccccagt
      961 tcagggagcg cgagcagaag gaggaggagg tgcgcgagct ggtcgcgctc gccgagcagc
     1021 tcgaaggcct gacgcgcaac gtcggcatgc acgcgggcgg cgtgctgatc gcgcccggaa
     1081 agctcaccga tttctgcccg ctctacacgc ccgacggctc cgacagcgtg gtgtcccagt
     1141 tcgacatgaa ggatgtcgag gcggtcgggc tggtcaagtt cgacttcctc ggtctcacca
     1201 ccctcaccat cctcgactgg acgctgcgat tcgtgcgcca gctcgacccc gcttcgaagg
     1261 tgaagctgga agagactccg ctcgatgacc ccgccagtta ccgcgtattc gcgcaggccg
     1321 ataccaccgg cgtcttccag tttgaatccc gcgggatgcg cgacctgctg gcgcgcgcga
     1381 aaccggaccg cttcgaagac atcgtggcgc tggtcgcgct ctaccggccg gggccgatgg
     1441 agctgatccc ggaattcatc aagcgcaagc acggcgggcg cgtcgattac ccggatccgc
     1501 gcgtggagcc gatcctcaag cccacctacg gcatcatggt gtaccaggag caggtgatgc
     1561 agatcgccca gatcatcggc ggctacagcc tgggcgcagc cgacctgctg cggcgcgcga
     1621 tgggcaagaa gctgcccgaa gagatggctc agcatcgcag cagcttcgtg gcgggcgcgg
     1681 cgaaaaacga cgtcggcgcg cgcagggccc acgagctgtt cgacctgatg gagaagttcg
     1741 cgggttacgg cttcaacaag tcgcacgcgg cggcgtacgc gctggtggcg taccagaccg
     1801 cctggttcaa ggcccaccac gcctcggcgt ttctcgccgc caacatgtcg gcggtgatga
     1861 acgacaccga caaggtcaag cagttcgtcg acgacacgca ggcgcacggc atcgtcctgc
     1921 gcgcgcccga catcaatgaa tcgaactacc gtttcgagcc ggtggacgcg aagaccatcc
     1981 gctacgggct gggggggatc aaaggcacgg gcgaggccgc gatcaattcc atcctctcgg
     2041 cgcgtaaaga ggggggggcg ttcagggacc tgttcgattt ttgccaccgc gtggacaagc
     2101 ggctcgtcaa ccgccgggtc gtggagtcgc tgatccgcgc cggcgcattc gactcgatca
     2161 acgatcatcg cgcgagcctg ctggcctcgg tcggcatggc gctggagagc gcggagcagg
     2221 cgagccgctc cgtcaaccag gtgagcctgt tcggggacct cacggacagc acgcagcaac
     2281 ccgctctcgc cggcgtgccg cgctggggcg ccaaggagcg gctgcagaac gagaagctcg
     2341 cgctcggctt ctacctgagc ggacaccttt tcaacatcta tcgagacgag gtgcgcggct
     2401 tcgcccgcac gcggatcggc gaactcgcgg cgctgtcgcc gggcgagtat gcgggtcgca
     2461 cctactgggt cgccggcgtg gtgatgggaa tgcgcatcca gaacaccgcg agcgggcgca
     2521 tgggggtcat cacgctggcc gacgactcgg acagcaacga ggtgatcttc ttcggcgagg
     2581 ccttcgacaa gttccgccag aagatcaggg aggatgagct gctggtggtg gaagtgcagt
     2641 tgcgcgcgcg cggcggcggc gggcgcatgg tcaacggcga gtacgagggc ggcggggagg
     2701 cgcgcgcgag catacgcgcg gtcaatgcgc tcgatttcaa cgaggcccgc aaccgcttcg
     2761 cgcgcggcgt gcggatcacc tgcaacggcg cctcgtcggg cggccggctg cgcgacctgc
     2821 tcgcgcctta ccgcagcggc cgctgtccgg tctcggtcgt ctattcgaac cgcggcgcgg
     2881 tctgcgagat cgacctgggc gagagctggc gcgtcaactt gcacgacgat ctcatccact
     2941 cgctgtccga gtggctgagt cccgagaacg tcaagatcgt ctacaacgaa tcgcgtgctg
     3001 accagtaaat ttattgaccg ccaagaaaat ggaaatgaaa ccgcagccat acgcaggtca
     3061 agtcaaaaac agccagccac agagagcacg gagggtccaa gagtcgattc accaatcacc
     3121 catcaccaat cacgcctttg cagcagtgct gtcgctattg ctcgccgctt cgccggctgc
     3181 cgccggcgaa gcggagtgga agatgttcta cgagcagtcc gccgcgcacc tgcagcaggg
     3241 cagcctggag caggccgagc tgttcgcgcg cgaggcgctg cgcgaatcgg aactcctgcc
     3301 gggcaagtct cgcgcaaccg agctttccct gctgcgcgcg gcgcacgtcc tgaggctgcg
     3361 cggcaaggcg gacgaggccc tgccgcttgc ggagcgtgcc gtgaagatca gcgcgcaact
     3421 gcatggcgcc aacagccccg acacggctgt tgccctgcag atccaggccg aagtgcagtc
     3481 cgcacgcagg cagtattcag cggcggaggc gctccaccgc aaagcgctct ccgtgttcca
     3541 gaagacctat ggcgaccggc atttgagtgt cgccgtgtcg ctgcacaacg tcggcgcgat
     3601 gctctacgcg caggacaaga acaaggaagc cgagaaatac ctgcggcgcg cgctggtggt
     3661 aaaggaacag gtgctgaagc ctgggaacct cagcattgca catacgctcg accagctgtc
     3721 gatcgtgctg gcggtacagg ggcgcgaaat cgaggcggaa cgttaccgga agcgggcgga
     3781 ggggatacgg cggcgcgttc aggcgcccaa gccgagcgcc tgagcgcgaa ggtaaaaggc
     3841 aaaagggaaa aggcaaaagg gaaaaggagc ccggaaaccg cgacaccttt cccttttacc
     3901 ttttcacttt tcccttaagg ttattcggtc ggcagcgtct cgaatccgct gaattctccc
     3961 gtcccgactt cgacctgcac ctcggcgacc acggcgctcg gcgggccgcg ccgtacccag
     4021 gcgatcatcg cctccacggc gctcatgtcg ccctggaccg tcgcttccac gctgccgtcg
     4081 agccggttgc gcacccaacc ggccaccccc agttcgcgcg cctttctctg catgtaaaag
     4141 cgataaccca cgccctggac gcgtcctgtg atgatcaagt gccgggtaac tcgttccatg
     4201 agcggtcatt ccgccggacg ggtgagccgc ccttatcata ggacaagcag ccgcatttgg
     4261 tgaacggcgg ctattgcggc attcccgtac gtgccgccat gcgtctaggc tgccgggcac
     4321 gcgccggtta ccgggcacgt taaataattt attcttgtaa ttcttggcgg ccttggcgtc
     4381 ccttcgactt gctcggggca ggcttggcgg ttcgattttc gttttcccgg cgccctccgc
     4441 ggtgaaaggt ttgcttagag agcgattgag cgcatagaat cgtgcttttg ccgcgcgacg
     4501 cgaaagcaca atggacgcgc ggccgaccgc acatagccga tgcccgctgc caccgctatt
     4561 tcgccggaac atgtcatcgg catcgtgttg ttccggctgg cgaccgggat tgcgagcggc
     4621 acggccgctc agcgcgtgta accgctggta acctcgaatg aattccgttt tcttccaacc
     4681 gatcgaggcg gcggtcacgc tgtcggtgat ctggatcggc ctgggcgtga tcggcctggc
     4741 gttcacgcgc gcgccgcgga tgatcaccca tgccgtattt cctgccggag cgctggtttc
     4801 gctattgctc gcggcgaccg ggttgtgggc gatcggcgcc gcccccagca cggccatcct
     4861 gccggctggc ctgcccgacc tgccgttcca cgtccgcatc gatgccctgt ccgggttctt
     4921 cctgctgctg ctgggcgggg tctcgttcgg tatttcgctg tttgccgtcg gctactttcg
     4981 cgccaccggg ggcacggcgc tcgggctgct gaacctgcag taccacgtgt ttctcgccag
     5041 catggctttc gtgctgatcg ccgacgacgc ctacctcttc atgatcacct gggagtcgat
     5101 ggcgctctcc tcgtacttcc tcgtcaccac cgagcaccac gaggagcaga accgcaaggc
     5161 cggcttcatc tatctgctgg tcgcgcatat cggcgcgatc agcatactgc tgtgcttcgg
     5221 cgtgctgcac ggcggccacg gcgactacac gttcgatgcg ctgcgcgcgg cgaatctggg
     5281 cggatcctgg gcgaccgtgg cgttcctgct ggcatttttc ggtttcggcg ccaaggcggg
     5341 catgctgccg ctgcacgtat ggctgccgga ggcgcatccg gccgcgccgt cgccggtgtc
     5401 cgccctgatg agcggcgtga tgctgaagac ggcgatctac gggatggtac gcgtcatcta
     5461 cgatctgatc ggcggcatcc gctgggagtg ggggctcacc gtcctgctgt tcggtgcggc
     5521 cacctcgctg ttcggcgtgc tgtttgccct gatgcagcac gatttgaaac gcctgcttgc
     5581 gtaccattcc gtcgaaaaca tcggcatcat cctgatcggc atcgggctgt cgatggtgtt
     5641 tatcggcagc ggccacccgg tcgccggcgc gctcggcctg atcgccggtc tttatcacac
     5701 gctcaatcac gcggtattca agggcctgct gttcctcggc gccggttcga tcctgaagtc
     5761 caccggattg aggaacctga acgagatggg cgggctgatc cgccacctgc cgcatacggc
     5821 gctctatttc ctgatcggcg cgctggcgat ttcggcgctg ccgcctttga acggttttgt
     5881 atcggaatgg ctcacgttcc aggcttcgct gcaggcaccg ctgctggaca gcagcgtggt
     5941 gaggagcctg gtgccgatcg tggccgccgt gctcgcgctc tcgggggcgt tgaccggcat
     6001 gtgcttcgtc aaggtctatg gcgtcgcctt tctcggccag cagcgccacg attttgtgga
     6061 ggcgccgcgc gaggcaagcg gctgggaacg cgccggaatg ctgtggcttg cgctcgcttg
     6121 cttcgcgctc gggctgtttc cggtgttcat gatcggcgaa ctcaacaagg tcagccttgc
     6181 cttgatcagc accgggctga acgaggaggc aatggccgcg ggctggctgt ggctcgcgcc
     6241 gacttccagc gcgcaggcga gctacagccc ggtcgtgttc ctgctggtga tcacggcggt
     6301 gctcgggttg acctttatgg cggtgcgcgc catctaccac gggcgccttc gccgcgtcga
     6361 cccgtgggat tgcggcttcc cggcgcaaac gccgcgcatg caggacagcg cggacgcgtt
     6421 tggccagccg atccgccaga tctttgagcc ggtctaccgg atccggcgtg aaattccgcg
     6481 tgccgatgac acgcatccgg tattcaccca atcggtggaa gaccgccact ggtacgcgat
     6541 gtatctgccg atcgcgcgct ttaccgagtt tttgtccggc cacgtcggca ggctgcagca
     6601 ggggcgcatt tcggtttacc tgctgtacag cttcctgacc ttgatcgcgc tgctggtgtt
     6661 cgcgcaatga acattacggg cgcaatcgtt tttcaggtgc tgcaatcggt gctggtggtg
     6721 ctgatcgcgc cgctgatgct gggctgggtc aaccagtgcc gcgcatggct gcagaacagg
     6781 agcggagcgg gaatctggca gccgtacaag gtgctgcaca agctgttcca caaagacgcg
     6841 gtgctcgcgc acaatgcctc gcccctgttt cgcgtcacgc cttatatcct gttcggctgc
     6901 atgtggctcg cggtcggcat cgtgccggtg ctggcgacct cgctgccctt tgcgccggcg
     6961 gccgacgtga tcgcgctggt cggactgttc gcgctggcgc gcgtgttcat ttcgcttgcc
     7021 ggcatggacg tcggcacttc gttcggcacg ctcggcgcgc gccgcgagat gatgatcggg
     7081 ttcctggccg agccggcgat gctgatgacg ctgttcacgg cttcgctcat cagccagtcc
     7141 acctcgctcg cgaccatcgt cgaaacgctg ggccatcgcg agctcgtgat ttatccgagc
     7201 atggcgttcg cggcggtggc gttcctgatg gtgctgctgg cggaaaatgc gcgcgttccg
     7261 atcgacaacc cgtccacgca cctcgagctc accatgatcc acgaagcgat gatcctcgag
     7321 tactcggcgc gccacctggc gctgatcgaa tgggcggcga gcctcaagct gaccgcctac
     7381 gtcacgatcg gcatcgccct tttcgtcccg tgggggatcg ccgggcccgg cgactggcag
     7441 gcgctgccgc tcgcgctggc tgcgctcacg ctgaagctgg tcgcggccgg agccgggctc
     7501 gcgttgctcg agacggtgtc ggccaagatg cgcatcttcc gcgcgcccga gtttctcggc
     7561 accgccttca tgctggccac gctcggcatg ctcatccatt tcctgctgga gcgatagtca
     7621 ggccatgaat ctgccgcttc acgcacagct gatcaacctg ttcgcggcgc tgatgctgct
     7681 gcttgcattc gcgatgctca cgcagcggcg catcctgtcg ctcatcaacc tgtttgccgc
     7741 gcagggcttc gtgctggtgc tctccacgct ggtggtcgcc tacttcacca accagggcca
     7801 cttgtattac tccgcgctgc tgacgctggc gctcaaggtg ctgctgctgc cgtggatcat
     7861 gcaccgcctg atacgggcgt tgaacgtgca atgggacgtc gagaccctga tcaacatccc
     7921 ggcaacgatg ctgatcggca tcgtgctggt ggtgttcgcg ttcaacctcg ccgacccgat
     7981 ttcccagttc gccggcacca tcaccaagag cacgctcggc atcgccaccg cctgcatcct
     8041 gctggccttc ctgatgatga tcacgcgccg caaggcggta tcgcaggtga tcgggttcct
     8101 ggccatggaa aacggcctgt ttttcgcggc aacgtccgcc acctacggca tgccgatggt
     8161 ggtcgagctc ggcatcgccc tcgacgtgct ggtcgccacg ctgattttcg gggtgttctt
     8221 cttccatatc cgcgagacgt tcgagagcct cgacatacac cacctcgaga agctgaaaga
     8281 acgctgatgg aacttctgct gttactgggc gctccgttcg ccggcggcat cctgctcgga
     8341 ttctgggggc atcgcaattt cgcgccggaa ctcaactcgg gcatgagcct gttgacgttc
     8401 ctggcggcgg cggcgctcac ggtgcgcatc atcgccgacg gaccgatcct ggtgtgggac
     8461 gagatgttct tcgtcgactc gttcaacgtg ttcctggtgg cgctcacggc gttcgtcgcc
     8521 ttcaccacct cgttgttcag ccggccgtac atgcgcatcg aactcgccct cggcaagctg
     8581 tcgccgaacc ggctgcggct ctaccacagc atgtaccaga tgttcacctt caccatgctg
     8641 ctcgtgctgc tgaccaacaa catcggcatc ttgtgggtgg cgatggaggc ggccacgctc
     8701 accaccgtgc tgctggtgag cctgtaccgc acgccggcga gcctggaggc ggcgtggaag
     8761 tacttcatcc tgtgcagcgt ggggctggcg caggcattgt tcggcaccat cctgctgtat
     8821 ttcgcggcag aaaaggtgct gggcgcgggc ggcaacgcgc tgctctggac gcatttgtac
     8881 ggggtgaagg atcagctcga gccgaccgtg ctggggatcg cgttcgtgtt cctgctggtc
     8941 ggctatggca ccaaggtcgg gctggcgccg ctgcacaact ggctgcccga cgcgcatgcc
     9001 gaaggcccga cgccggtgtc ggcggtgctg tcggggctgc tgctcaacgt cgcgctgtat
     9061 gcggtcgtgc gcaccaaggt gctggtcgaa ggcgcgctcg gtacccattt cgccggttac
     9121 ctgatgatgg gtttcggcct gctgtcggta gtggtggccg cgttcctgct gtcgcgccag
     9181 aaggacgcca agcgcctgtt cgcctactcg tcaatcgagc acatggggct catgaccttt
     9241 gccttcggca tgggcggggc cctggcgacc tttgccggac tgctgcacat gacggtgcat
     9301 tcgctcacca agtcggcgat ctttttcacc gtcggccacg ccgcgcagaa atccggcacg
     9361 caggtcatgg ccaacatcag ggggctgatc gacagcaacc cgacgatcgg ctggggactg
     9421 atgctcggca gcctggcgat actcggcata ccgccgttcg gcgtgttcgc gagcgagttc
     9481 atgatcctga cggtgacgat gcacgagcag ccgtgggcca cgcctttcct cctggtcgcg
     9541 ctcggcgtgg cattcgccgc gatattcggc aaggtccagc cgatggtgtt cggcaccgcg
     9601 accgccgagc gcctgccgca tccgccggcg ctggtgccgg tgttcgtgca tctcgcgata
     9661 gtgctgttg
//
LOCUS       DBNGSG010000097         4034 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1390218, whole genome shotgun sequence.
ACCESSION   DBNGSG010000097 DBNGSG010000000
VERSION     DBNGSG010000097.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4034)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1390218"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..1026
                     /locus_tag="AC158Q_02475"
     CDS             55..1026
                     /locus_tag="AC158Q_02475"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3338663.1"
                     /translation="MKHRGFTQALLVAAAFTAVPAVMAQAYPSKPVRFLVGFPPGGTN
                     DIVARIVAPKLSEFLGQQVVVENRGGANASIAAELTARAAPDGHTIMVVAPGHATNPA
                     LLKLNFDSLKDFAFITQLAESQNLLVVHPSFPPRNVKELVAFSKKHPAGTINYGSSGT
                     GTTVHLSAELFQFMTGAKWLHIPYKGGGPGVVALLSGEVSLYFGNVPTVIRQARAGKL
                     RALATTGSKRTPAAPDIPTVAEAGVPGYEVTTAYGMAAPAKISRAILDRLHADIVRTL
                     NSPEVRDKLQGLGADPIGNTPEQYTAFVQNEIAKWGKVIKAAGIKGE"
     gene            1083..2021
                     /locus_tag="AC158Q_02480"
     CDS             1083..2021
                     /locus_tag="AC158Q_02480"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3338664.1"
                     /translation="MPFAFCLGIACANAQSYPAKPIRFLIGFPPGGTNDILARALAPK
                     LSESLGQQVVVENRGGASTAIATELAARSAPDGYTIILNAPGHATNPALKKLPFDSVK
                     DFAFITLLAESQNLLTVHRSLPVTSVKSLIAISKQRPGDINYGTSGIGTTPHMSAELF
                     QFMTGVKWVHIPYKGSGLGLTALLSGEVSLYFANIPSVIRHVQAGKLRPIALSGPRRS
                     AAAPGIPTVAESGIPGFAVTSWFGVAAPARTPRPIVDRLNSEIVRALNTPDLRERLLG
                     MGADPVGNSPEQYTAFVQNEIAKWARVIKAAGIKGE"
     gene            2061..3047
                     /locus_tag="AC158Q_02485"
     CDS             2061..3047
                     /locus_tag="AC158Q_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3338665.1"
                     /translation="MKSIRHRSRVTSRALAAALLLAASAAAAQGYPAKPIRIIVPFPA
                     GGIADVFGRVIGQKFTEAWGQPAVVENRPGAGGNIGAEIVAKSPADGYTLVTGSIGTH
                     SVNVSLFSKLPYDPIRDFAPVSLIMEAEGLLVLHPSVPARTVKELIALARARPGQLSY
                     ASAGHGTASHLSGELFKSMARVNMIHVPYKGNVPAITDLLAGQTSLLFATMPTVLPQV
                     QAGRLKALAVTSSARAPAAPQLPTIAEAALPGYSVTNWIGVFAPAGTPRDVVVKLNRE
                     IVRIMQAPDIQKRLANEGAKFTPLTPDEFTAFVKAEIAKWAKVVREAGIRVD"
     gene            3100..>4034
                     /locus_tag="AC158Q_02490"
     CDS             3100..>4034
                     /locus_tag="AC158Q_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HIR3338666.1"
                     /translation="MKQLEEKNAEALAMGGPEKLARRKAEGHLNARERIDYLVDPGTF
                     VESGRYARAIREEVRHKTPADGKVAGFARIAGREVSLVSNDFTVLGASSSVINMKKIR
                     HVKAAAARYGMPLVFLGESSGARMPDRMGAAGRAILAQDPTEYQRLRETPWVSTLMGQ
                     CYGSSTWYAAMSDFVVMRKGSVMAIASPNVTSIAIGKPIDPEELGGWKLLTGSSGLAD
                     LAVDTDEQALDAVKRFLSYMPAHCMEAPPEHPVPAGSNEACRGILEIVPEERNKVYDV
                     RKAISAIVDTGSSFELKERYGRSVATVLARLDGKSV"
BASE COUNT          744 a         1308 c         1344 g          638 t
ORIGIN      
        1 ccgggcaacg cggagccggc gtaaaggtgg agtacttatg cggaggaggc agagatgaaa
       61 caccgaggat tcacgcaggc actgttggtc gcagcagcgt tcaccgccgt gccggccgtc
      121 atggcgcagg cctatcccag caagccggtc cgcttcctgg tcggtttccc cccgggcggc
      181 accaacgaca ttgtggcacg catcgtcgcg cccaagctat ccgaattcct cggccagcag
      241 gtggtggtcg aaaaccgggg cggcgctaac gcctcgatcg ccgccgaact caccgctcgc
      301 gctgcgccgg atgggcatac catcatggtg gttgcgcccg gccacgccac caacccggcg
      361 ctgctcaaac tcaatttcga ctcattgaag gatttcgcat tcatcacgca actggcggaa
      421 tcacagaatc tgctggtggt gcatccgtcg ttcccgccac gaaacgtaaa ggagctggtc
      481 gcgttttcga agaagcaccc ggcgggcacc atcaactacg gctcctccgg gaccggcacc
      541 acggtgcacc tgtccgcgga actgtttcag ttcatgacgg gcgcgaaatg gctgcatatc
      601 ccctacaagg gcggcggacc aggggtcgtc gccctgctct ccggtgaagt ctctctctat
      661 ttcggcaacg tgcccacggt catccggcag gcgcgcgctg gcaagctgcg tgcgctggcc
      721 accaccggat caaaacgcac gccggcggcg cccgacatcc cgacggtggc ggaagccggg
      781 gtgccgggct acgaagtgac cactgcctac ggcatggctg cgccggcaaa gatatcgcgc
      841 gccatcctcg accggctgca tgcggatatc gtgcgcacgc tgaattcgcc cgaggtgcgg
      901 gacaagctgc agggattggg cgccgatccc atcggcaata cgccggaaca gtacaccgct
      961 ttcgtccaga acgagatcgc caagtggggc aaggtgatca aggcggcggg aatcaaaggc
     1021 gaatgatgaa aggcaaggta caaggtagaa gcggccaggt gaaagggttt tccttttgcc
     1081 ttttgccttt tgccttttgc cttgggatag cctgcgcaaa cgcgcagagc tatcccgcca
     1141 agccgatccg tttcctcatc ggctttccgc cgggcggcac caacgacatc ctggcgcgcg
     1201 cgctcgcgcc gaaattgagc gagtccctcg gccagcaggt ggtggtcgag aaccgcggcg
     1261 gcgccagcac ggcgatcgcc accgagctcg cggcgcgctc ggcgccggac ggctacacca
     1321 tcatcctgaa cgcacccggc cacgccacca atccggcgct gaagaaactt cccttcgatt
     1381 cagtgaagga tttcgctttc atcacgctgc tggccgaatc gcagaacctg ctgaccgtgc
     1441 accggtccct gccggtgacc tcggtgaaat ccctgatcgc gatctcgaag cagcggccgg
     1501 gcgatatcaa ctacggcacg tccggcatcg gaaccacgcc ccatatgtcg gccgagctgt
     1561 tccagttcat gacgggcgtg aagtgggtgc acattcccta caagggcagc gggctgggat
     1621 tgaccgcgct gctgtcgggc gaggtgtccc tttatttcgc caacatacct tcggtcatcc
     1681 ggcacgtgca ggcgggcaag ctgcggccca tcgcgttgag cgggcccagg cgctcggcgg
     1741 ccgcacccgg catcccgacg gtggcggaga gcggcatccc cgggttcgca gtcacgtcgt
     1801 ggttcggggt cgccgccccc gcccggacgc cgcgccccat cgtcgaccgg ctgaacagcg
     1861 agatcgtgcg cgcgctcaat acaccggacc tgcgtgagcg cctgctgggc atgggcgccg
     1921 atcccgtcgg caatagcccc gagcagtaca cggcgttcgt gcagaacgaa atcgcgaagt
     1981 gggcccgggt gatcaaggcg gccggcatca agggagagtg accatttgaa ggcgaaaggc
     2041 gaaaggcgaa aggcggaagt atgaaaagca tccgtcaccg gtcacgggtc acaagtcgcg
     2101 cgcttgcggc agcgttgctg ctggcagcaa gcgctgctgc cgcgcagggc tatcccgcca
     2161 agccgatccg gatcatcgtg ccgtttcccg cgggtggcat cgcggacgtg ttcgggcggg
     2221 tgatcgggca gaaattcacc gaagcctggg ggcagccggc ggtggtggaa aaccgccccg
     2281 gtgccggcgg caatatcggc gccgagatcg tcgccaagtc ccccgccgac ggctacacgc
     2341 tggtcacggg cagcatcggt acgcactcgg tcaatgtcag cctgttcagc aagcttccct
     2401 acgacccgat ccgggatttc gcgccggtgt cgctgatcat ggaagccgag ggcctgctgg
     2461 tgctgcaccc gtcggtaccg gccagaacgg tgaaagagct gatcgcgctg gcgcgggcgc
     2521 ggccgggcca gttgagctac gcatccgcgg gccacggcac cgcgagccac ctgtccggcg
     2581 agctgttcaa gtccatggcc agggtgaaca tgattcatgt cccgtacaag ggcaacgtcc
     2641 ccgcgatcac cgatctcctc gcgggccaga cctcgctgct gttcgccacc atgccgacgg
     2701 tgctgccgca ggttcaggca ggccggctga aggcgctcgc ggtgacgagc tccgcgcgcg
     2761 cgccggcggc gccgcaattg cccaccatcg cggaagctgc gctgccgggc tactcggtca
     2821 cgaactggat cggcgtgttc gcgccggccg gcacgccgcg ggacgtcgtc gtgaagctca
     2881 acagggagat cgtgcgcatc atgcaggcgc ccgacatcca gaagcggctc gccaacgaag
     2941 gcgcgaagtt cacgccgctc acgccggatg aattcaccgc gttcgtcaag gcggagatcg
     3001 ccaagtgggc gaaggtcgtg cgagaagccg gcatacgcgt tgattgagag ccgtgatggg
     3061 tgatgagata aaaggcaaac catgccggct agagacttgt tgaaacaact ggaagagaag
     3121 aacgccgagg cgctcgccat gggcgggccg gagaagctcg cgcggcggaa agccgaaggc
     3181 cacctgaacg cgcgcgagcg catcgactac ctggtcgacc ccggcacctt tgtcgaatcc
     3241 gggcgctacg cgcgggcgat ccgcgaggag gtgcggcaca agacgcccgc cgacggcaag
     3301 gtcgcgggct tcgctcgcat cgcggggcga gaagtgtctc tcgtgtccaa cgatttcacc
     3361 gtgctgggcg catcaagcag cgtcatcaac atgaagaaga tccggcacgt gaaggccgcg
     3421 gcggcccgct acggcatgcc gctcgtgttc ctcggcgaat cgtcgggcgc gcgcatgccc
     3481 gatcgcatgg gcgctgcggg ccgtgccatt ctcgcgcagg acccgaccga gtaccagcgg
     3541 ctgcgcgaga cgccgtgggt gtccacgctg atgggccagt gctacggttc ctcgacctgg
     3601 tacgccgcga tgtcggactt cgtcgtgatg cgcaagggct cggtgatggc catcgccagt
     3661 cccaacgtga cctcgatcgc gatcggcaag ccgatcgacc ccgaggaact gggcggctgg
     3721 aagctgctga ccggcagctc gggcctcgcc gacctggcgg tggacaccga cgaacaggcg
     3781 ctcgacgccg tgaaacgatt tctttcctac atgccggcgc actgcatgga agcgccgccc
     3841 gagcacccgg tgccggcggg gtcgaacgag gcgtgccgcg gcattctcga gatcgtgccg
     3901 gaggagcgca acaaggtcta cgacgtgcgc aaggcgatct cggcgatcgt ggacacgggc
     3961 tcgagcttcg agctgaaaga gcgctacggg cgctcggtcg cgacggtgct ggcgcgtctc
     4021 gacggcaaga gcgt
//
LOCUS       DBNGSG010000098         5182 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4617601, whole genome shotgun sequence.
ACCESSION   DBNGSG010000098 DBNGSG010000000
VERSION     DBNGSG010000098.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5182)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5182)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5182
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4617601"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="AC158Q_02495"
     CDS             complement(<1..671)
                     /locus_tag="AC158Q_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338667.1"
                     /translation="MSDTPGLVRSSVKGMLWPGLPSNGVTLAMQYQLEQSQWWSAGQL
                     EQHQLKQLSLLLRHAYRQIPFWRERLEGAGYGPEERPTSQWLSTLPLLDRHDIQTLGD
                     SLLCKEVPRGHGRITKGETSGSTGRPIVHYGTELTQFFWRVYTLRDHLWHRRDLSATL
                     AAIRKNAQEAPRKGWGPATDVAFEAGMGLGFDLKNDIDTQLRWLQRTKPEYLITAAYN
                     LYWLAR"
     gene            796..1668
                     /locus_tag="AC158Q_02500"
     CDS             796..1668
                     /locus_tag="AC158Q_02500"
                     /inference="COORDINATES: protein motif:HMM:NF025033.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3338668.1"
                     /translation="MNSVEEPLAESAPIAREFASRLCYRDPGTGETCSWYHGLWQFLR
                     LLKLVTAPEHHADFYREALGEIANGAGPYRVLVSGAADYSMLAYALAAFRSQAKEPEL
                     SVNDLCETPLALSRWYSDRVGCRINTLRCDVLQLPEESQFDVVCTHSFLGRFPHEKRV
                     QLVEKWRRVLRPGGRVVTVKRIRPGSSGEPTGFSPAEIQAFRDKVLNAAGALRASHAI
                     DPLELAEEARIWASRRRTWPVRSSEEILALFESSGFKVDRLSCSPVRSTRSGLSGPST
                     LGSADYAQIVATRL"
     gene            complement(1697..3058)
                     /locus_tag="AC158Q_02505"
     CDS             complement(1697..3058)
                     /locus_tag="AC158Q_02505"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HIR3338669.1"
                     /translation="MTAPDWQYYSAVPGIGWPAIPGKAPAALLALQFQLERSQWLEPE
                     KLLDFQFRQLEVLLRHAYATVPYYREHWHELYDPRQPLDAGRFSRLPLLTRSDLQGNF
                     DALKSSDVPATHRPVGEARSSGSTGMPVRVLKSGLTRLLWQAALLREHVWHRRDLSGR
                     LAVIRHGVKAGEANTWGTPLRGLAKTGPAVQLPTSVAVGAQLRWLEEQQPDYVLTYPS
                     NLNELLRLSLERGTRLPRLREARTLGEVVPPELRALCREAWGVPVVDAYSAQEVGYFA
                     LQCPDQEHYHVQSESVMLEVVDAQGRACVPGQAGLIVVTPLHNFAMPLVRYVMGDYAE
                     VGERCGCGRGLPVLARIMGRVRNMLVTAAGERYWPSFGTRKLTNIAPILQHQFVQKSH
                     DLIEARLVMAAPLTLEQEDELRQHVLSRLPAGFQMNFVYCQQIPRSAGGKFEDFISEV
                     ATA"
     gene            complement(3166..4362)
                     /locus_tag="AC158Q_02510"
     CDS             complement(3166..4362)
                     /locus_tag="AC158Q_02510"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HIR3338670.1"
                     /translation="MTVRPAALKNPQPAPAPEAEQPQLFDTHPEVRLDFKHVRKALKQ
                     GYTRPFLLVDTGIVRTKVRRFKSAMPRVHPHYAVKANPDPRVLKVLIEEGAGFEIASI
                     AELDTLLELGVPAAEVYYSNPMKSRAYIEYAAAKGVEWFVLDSVEELRKIHNIKRDAK
                     LYVRIEAPNVGSDWPLAGKFGMKPGEVDGVIAEAAALKADLAGVTFHVGSQCRNPENW
                     RVGIQSAKRVFKKMRLAGLNPRLLNLGGGYPVRHVKPIPSIEKIAEVVNYALRDMPQS
                     VRVMAEPGRYFVSDAGYFVCRVVGTATRSGKRWMYWDAGVFGGVIETTEGLRYDVQTD
                     RSGKLIPWNVAGPTCDSVDVCMRDEMYPEDLREGDFIYIANAGAYTTAYASNFNGFPL
                     PEVRVI"
     gene            complement(4883..>5182)
                     /gene="dcd"
                     /locus_tag="AC158Q_02515"
     CDS             complement(4883..>5182)
                     /gene="dcd"
                     /locus_tag="AC158Q_02515"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HIR3338671.1"
                     /translation="ARTVEYFRIPRNVLTICLGKSTYARCGIIVNVTPLEPEWEGYVT
                     LEFSNTTPLPAKIYANEGVAQVIFLEAQADDVCETSYKDRGGKYQGQVGVTPPKV"
BASE COUNT          958 a         1600 c         1666 g          958 t
ORIGIN      
        1 cgcgccagcc agtacaaatt gtaggcagcc gttatcaggt actcgggctt ggtccgctgc
       61 agccagcgca actgcgtgtc gatgtcattc ttgaggtcga atcccaggcc catgccggct
      121 tcgaacgcga catcggtggc aggcccccag cctttcctcg gcgcctcctg ggcgttcttg
      181 cggatcgcgg ccagcgtggc ggacagatca cgccggtgcc acagatgatc gcgtagcgta
      241 tagactcgcc agaagaactg agtaagctcg gtgccgtagt gaacgatggg cctgcctgtc
      301 gagccggagg tttcgccttt cgttatccgg ccgtgcccgc gaggcacttc cttgcagaga
      361 agcgaatctc ccagggtctg gatgtcgtgc cggtcgagca atggcagcgt ggagagccat
      421 tgcgaagtcg gtcgctcctc cggcccgtat cccgcgccct caaggcgctc gcgccagaac
      481 ggtatctgcc gataggcatg gcgcaacagc agggagagtt gcttcaattg atgctgctcg
      541 agctgcccgg ccgaccacca ttggctttgc tcgagctgat attgcatggc cagcgtgacg
      601 ccgtttgaag gcaggcccgg ccacagcatg cccttcacgg aggagcgaac gagaccagga
      661 gtgtccgaca caaatcgatc gtcgtgcaaa gcgcgcagtt tagcatcgcg acgcggcccg
      721 gaggctcgac tcccgctttc ggcgcacgta tgatgcgcag ccagacacta cacgatgcgg
      781 cgtgacgaac aaaccatgaa cagcgtggaa gaaccactgg cggaaagcgc cccgatcgca
      841 cgggaattcg cctcgagact gtgttaccgc gatcccggca ccggcgagac ctgttcgtgg
      901 tatcacgggc tctggcagtt cctgagatta ttgaagcttg tcactgcccc ggaacaccat
      961 gcggatttct atcgtgaagc cctgggcgaa atagcgaacg gcgcaggacc gtaccgggtg
     1021 ctggtttcgg gcgccgccga ttactccatg ctggcgtacg cgcttgcggc gtttcgcagc
     1081 caggcaaaag agcccgaatt gtcagttaac gacctgtgcg aaacacctct tgcactgagc
     1141 cgctggtatt cggaccgcgt cggctgccga atcaacacgc ttcgctgcga cgtgctgcag
     1201 ctcccggagg agagccagtt cgacgtagtg tgcacccact cgttcctggg ccgctttccg
     1261 cacgaaaagc gcgtgcaact cgtggagaaa tggcggcgag tgctgcgtcc cggagggcgg
     1321 gtggtcacgg tgaagcggat caggccggga agcagcgggg aaccaacggg tttttctccc
     1381 gccgaaatcc aggcgtttcg agataaggtt ctgaacgccg ccggcgcctt gcgcgcgtcg
     1441 cacgccatcg accctctcga attggcggag gaagccagga tatgggccag ccgtcgccgc
     1501 acctggccgg tacgttcgag cgaggaaatt ctggcgctat tcgagagctc gggcttcaag
     1561 gtcgatcgcc tttcctgctc ccccgtccgc agcacccggt ccggactcag cggcccatcc
     1621 acgcttggaa gcgccgatta tgcgcaaatc gtggccactc gactgtaact ctcgatgaga
     1681 cgcgccccat cccgcctcat gccgtggcaa cctcggagat gaaatcctcg aatttgccgc
     1741 cggcgctgcg cggaatctgc tggcagtaga cgaaattcat ttgaaagccc gcgggcagcc
     1801 gggacagaac gtgctgccgc agttcatcct cctgttcaag tgtcagtgga gcggccatga
     1861 ccagccgcgc ctcgatcagg tcatggcttt tctgcacgaa ctggtgctgg agtatcgggg
     1921 caatattggt cagttttcga gtgccaaagg acggccagta gcgctccccg gctgcggtta
     1981 ccagcatgtt gcgtaccctg cccatgatac gggccaatac cggaagaccc ctgccgcagc
     2041 cgcaccgctc gccgacttcg gcgtaatcgc ccatcacgta acgcaccaac ggcattgcaa
     2101 agttgtgcag tggagtaacc actatgagtc cggcctgccc ggggacgcac gcccttccct
     2161 gagcatcgac gacctcgagc atcacgctct cggactgcac gtggtaatgc tcctggtcag
     2221 gacactgcaa tgcgaagtag cccacttcct gggccgaata ggcgtccacg accggcacgc
     2281 cccatgcttc ccggcacaat gcgcgcaact ccgggggcac gacctcgccc agggtccgcg
     2341 cttcgcgcaa acggggcagg cgtgttccac gttccaggga gagtctcagg agttcgttga
     2401 ggtttgacgg gtaagtgagc acgtaatccg gttgctgttc ctcgagccag cgcaattggg
     2461 cgcctaccgc tacactggtt ggcagttgca ccgcgggccc ggttttcgca aggccacgca
     2521 ggggggtgcc ccaggtattt gcctctcccg ccttcacgcc atggcgaatg accgcaagcc
     2581 tgccgctcaa atcgcgccgg tgccacacgt gctcacgcag caaggccgcc tgccatagga
     2641 gccgggtcaa gccggacttc aataccctca ccggcatgcc ggtagaaccg gaactgcggg
     2701 cttccccgac aggcctgtgc gttgccggga catccgagct cttcaaggcg tcgaaattgc
     2761 cctgcaagtc gctgcgggtg agcaggggca ggcgggaaaa acgcccggcg tcaagcggct
     2821 ggcgcgggtc gtacaattca tgccagtgct cgcggtaata cggcacggtg gcataggcgt
     2881 ggcgcagcag tacttcgagc tggcggaatt ggaaatccag cagcttttcg ggctcgagcc
     2941 actgggagcg ctcgagctga aactggagcg ccagcagcgc cgcgggcgcc tttccgggaa
     3001 tcgcaggcca gccgatgccg ggcacggccg aataatattg ccagtctggg gcagtcatct
     3061 tcaccggatg attgcaggag cgatcaggga cggtcaaccg cagggttcaa gcccttcaat
     3121 gaaaaagcgg gccgcagccc gcttttttcc ccctgagcgc ccttcctaga taactcgtac
     3181 ttccggcagc ggaaagccgt tgaagttgct ggcgtatgcc gtagtgtagg cacccgcgtt
     3241 ggcaatatag atgaagtcgc cttcccgcag gtcctccgga tacatctcat cgcgcatgca
     3301 cacgtccacc gagtcgcagg tggggccggc gacgttccag gggatcagct tgccgctgcg
     3361 atcggtctgc acgtcgtagc gcaggccctc ggtggtctcg atgacgccgc cgaagacgcc
     3421 cgcgtcccag tacatccagc gcttgcccga gcgggtggcg gtacccacca cccggcagac
     3481 gaaatagccc gcatcggaca cgaagtagcg accgggctcg gccatcacac gcacgctctg
     3541 gggcatgtcg cgcagggcgt agttcaccac ctcggcgatc ttctcgatcg aggggatggg
     3601 cttgacgtgg cgcaccgggt agccgccgcc gagattgagc agccggggat tcaagccggc
     3661 aaggcgcatc ttcttgaata cccgcttggc gctctggatg ccgacccgcc agttctccgg
     3721 attgcggcac tgggagccga cgtggaaggt aactccagcc aggtcggcct tgagggcggc
     3781 cgcctcggcg ataaccccat ccacttcgcc cggcttcatg ccgaacttgc cggcgagcgg
     3841 ccagtcgctg cccacgttgg gggcctcgat gcgcacatag agcttggcgt cgcgcttaat
     3901 gttatggatc ttgcgcaact cctcaacgct gtccagcacg aaccattcga cccccttggc
     3961 cgcggcatac tcgatgtagg cccgcgactt catcgggttg ctgtaataga cctcggcggc
     4021 cggcacgccc agttcgagga gcgtgtcgag ctcggcaatc gaggcgatct cgaagcccgc
     4081 accctcctcg atcagcacct tgagcacgcg gggatcgggg ttggccttga ctgcatagtg
     4141 cgggtggacc cgcggcatgg cggacttgaa gcggcggacc ttggtgcgga cgatgccggt
     4201 gtcgaccagc aggaaggggc gggtatagcc ctgtttaagc gccttgcgga cgtgcttgaa
     4261 gtccaggcgg acttcgggat gggtatcgaa gagttggggt tgttcggctt cgggggcggg
     4321 ggcaggttgc gggttcttga gggcggcagg acgcacggtc atcaatgctc tccttgagac
     4381 ctaggattcc ggtatgtctc gaatcagcac ttcagccgcg aggcggctgt agcttgccat
     4441 cgtcatcgtg cctgccttcg ttccttcgtt tccgtcacaa aaacgcaaaa cttccgcgaa
     4501 ttatagaagc gcggtaccag gaatcaagag ttttttcaag ttttcccgaa aattttccgg
     4561 tcgcgcgtgt cgtgtacgca acggtcgagt agctgtcggt ggcgccggaa agccggcggc
     4621 agagacgagc ggagagaatt attacagtcg ctccgagtga agcggcgtga cgactgacgg
     4681 gtgacgggta acgaggcgcc gaaccgctgg agacggccaa ctgaagccgg cgcgcagggc
     4741 agctcaggtg tgagacgggc gcggttttca agcagcccgg cgaacggcga ggcggccggg
     4801 ttgccgtgcg ttgagcggcc gcgggcctgc tcgaacggcg ggagacaaac cggcgcgtag
     4861 cgcccggctt taccgggctt tatcagactt tgggaggcgt gacgcccacc tgcccctggt
     4921 actttccgcc gcggtccttg taggaggtct cgcagacatc gtctgcctgg gcttccagga
     4981 agatgacctg ggcgaccccc tcgttggcgt agatcttggc cgggagcggc gtagtgttcg
     5041 agaactccag ggtcacgtag ccctcccact ccggctcgag cggggtgacg ttcacgatga
     5101 tcccgcaccg ggcgtaggtg gacttgccga ggcagatggt cagcacgttg cgcgggatgc
     5161 ggaagtactc gaccgtgcgg gc
//
LOCUS       DBNGSG010000099         1834 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4277476, whole genome shotgun sequence.
ACCESSION   DBNGSG010000099 DBNGSG010000000
VERSION     DBNGSG010000099.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4277476"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            297..1442
                     /locus_tag="AC158Q_02520"
     CDS             297..1442
                     /locus_tag="AC158Q_02520"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3338672.1"
                     /translation="MPIYLIVVSCVLIHAGFGGAKVALPLHALDLGVDPLTVGVMMAL
                     WALCPMLIALYVGRLVDRVGPRVPMLAGTVGVVVALLVPYFFPGVAALYVMALVVGTA
                     FQFFFVPVQGITGALGGPEDRARNYSLLAVGFSIASFLGPLIAGFSIDYLGYRAVYLM
                     LAAGPFLAVLLLVLKGKMLPKAAIGGGADGKKGSSFDLLRNSKLRDAIVASGLISIAW
                     DLYLFYFPIYGHSIGLSASAIGTVISTFAAAVFAIRVALPALTRRWSEFQILLYAIGF
                     AGAVFILFPLLRDPYLLAAASFLLGLGCGVGQPMSISLIYSLAPPGRASEGAGLRVTF
                     NHFTHLVVPLAFGGIGTAFGFAPVFFSCSAVLLGGSWFGHHSVKRAK"
     gene            1489..1830
                     /locus_tag="AC158Q_02525"
     CDS             1489..1830
                     /locus_tag="AC158Q_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338673.1"
                     /translation="MLRDGDIRAPAGARQRRSNELISFDLTLLARLSLEIAMRADRPA
                     FTEGDHAMSLKHTRGLSLLLAALFALLAGNVLADTRPAADGVIVAQSPTQDPNTPPDC
                     KKYPQDVRCKK"
BASE COUNT          234 a          618 c          599 g          383 t
ORIGIN      
        1 aaggcgaaag cggcgtccgc aggtttccga tttcgtcctc ctgcagcgtt gttagtcact
       61 cgtcactcgt cactcgtcac ccagcactcg cgccataacc attttgtgga ctaatcctgc
      121 ggtgtgaaac accggtggtt ccgaccgccg tcgattctcc tacggggaat ccggtttctg
      181 gtcctggcgt aatgttcacg atatggaatt ccgggcccgt cggcgcccga tcgcggtaga
      241 atgcgccgtc acgttttccg cacccgcggc gcgtatccgg cctcatttca tcgtccatgc
      301 cgatttacct gatcgttgtg tcctgcgtgc tgatccacgc cggcttcggc ggtgccaagg
      361 tggcgctgcc gctgcacgcg ctcgacctgg gcgtggaccc gctcacggtc ggcgtcatga
      421 tggcgttgtg ggcgctgtgc ccgatgctga tcgcgctcta cgtcggccgg ctggtggacc
      481 gcgtcgggcc gcgggtgccg atgctcgccg gaacggtcgg cgtggtggtc gcgctgctgg
      541 tgccgtattt cttccccggc gtggcggcgc tctacgtgat ggcgctggtg gtgggcaccg
      601 cgttccagtt cttcttcgtc ccggtccagg gcatcaccgg cgcgctcggc ggtcccgagg
      661 accgcgcccg caactactcg ctgctggccg tcggtttttc gatcgcgtcc ttcctggggc
      721 cgctgatcgc cggcttctcg atcgactatc tcggctaccg ggccgtttat ctcatgctgg
      781 cggccggccc gttcctcgcc gttctcctgc tcgtgctcaa ggggaagatg ctgccgaaag
      841 ccgcgatcgg agggggcgcg gacgggaaga agggcagctc gttcgacctg ctgcgcaact
      901 cgaagctgcg cgacgccatc gtcgcgagcg ggctgatctc catcgcctgg gacctgtacc
      961 tgttttattt cccgatctac ggccactcga tcggcctgtc ggcctcggcg atcgggacgg
     1021 tcatcagcac cttcgcggcg gcggtgttcg cgatccgggt agcgctgccg gcgctcaccc
     1081 ggcgctggag cgagttccag atcctgctct acgcaatcgg gtttgccggc gcggtcttca
     1141 tcctgttccc gctgttgcgc gacccctacc tgctggcggc ggcgtcgttc ctgctggggc
     1201 tgggctgcgg cgtggggcag ccgatgtcca tttcgctcat ctacagcctg gcgccgccgg
     1261 ggcgcgcgtc ggagggcgcc gggttgcgcg tgacgttcaa ccatttcacg cacctcgtgg
     1321 tgccgcttgc cttcggcggc atcggcaccg cgttcggctt cgcgccggtg ttcttttcct
     1381 gctcggcagt gctgctcggc ggcagctggt tcggccacca cagcgtgaag cgcgcgaaat
     1441 aagagcgcac ccgcccgcgc ggcttcaggg tccggcgcgc atctttgcgt gttgcgagac
     1501 ggcgacatcc gggcccctgc cggcgcgcgc cagcgcagga gcaacgaatt gatttctttc
     1561 gatttgacgc tcctggcacg gttgtcgctg gagatcgcga tgcgggccga ccggcccgcg
     1621 ttcactgaag gagatcatgc catgagcctg aaacacaccc ggggcctttc cctgttgctg
     1681 gcggcgctat tcgcgttact ggccggcaac gtgctggccg atacccgccc ggcggccgac
     1741 ggcgtaatcg tcgcccagtc ccccacccag gacccgaaca cgccgcccga ctgcaagaaa
     1801 tacccgcagg acgtgcgttg caagaagtaa cggc
//
LOCUS       DBNGSG010000100         3591 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4108374, whole genome shotgun sequence.
ACCESSION   DBNGSG010000100 DBNGSG010000000
VERSION     DBNGSG010000100.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4108374"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1170)
                     /locus_tag="AC158Q_02530"
     CDS             complement(<1..1170)
                     /locus_tag="AC158Q_02530"
                     /inference="COORDINATES: protein motif:HMM:NF025010.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR3338674.1"
                     /translation="MFDFIDKHKKLIQIVFLILIVPPFALFGVDAYFRGMEGGQAVAR
                     VGDHTITQEDFARTLRERQQSIQRTVQGRIDPEMLDNPELRQATLESMIQRRLLIDRA
                     LKSGITVSDSQLQSTISDLATFRDPAGNFSFEIYQQFLRNEGLTPATFEARIRQDMIL
                     QQFSSGYAENGFAPRTVVGRLARLAGEQREVSHATFAPEGYLAQVKLDPGATKQYYDA
                     NTAEFRIPEQARVEYVTLSVNSLMQEMQADPAEVKKYYDSNSRQFGVEESRQAAHILI
                     AVEPGAGEEAKKKARERADAIYNEVRKNPAGFAAAAKKHSQDPGSAAQGGDLGRVTRG
                     TMKDIPEFENALYQLKPGEISPPVETRHGFHVIRAVSVQPAQVKPFEEVRGQIEQE"
     gene            complement(1236..1312)
                     /locus_tag="AC158Q_02535"
     tRNA            complement(1236..1312)
                     /locus_tag="AC158Q_02535"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1276..1278),aa:Asp,seq:gtc)
     gene            complement(1406..1481)
                     /locus_tag="AC158Q_02540"
     tRNA            complement(1406..1481)
                     /locus_tag="AC158Q_02540"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1446..1448),aa:Val,seq:tac)
     gene            complement(1662..1934)
                     /locus_tag="AC158Q_02545"
     CDS             complement(1662..1934)
                     /locus_tag="AC158Q_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR3338675.1"
                     /translation="MNKADLVEVVSRSAGISKSASEKALDGAIDAIKGALKRGQSVTL
                     VGFGTFRVGKRQARSGRNPRTGAEIRIQAAKVPKFSAGKALKDAVN"
     gene            complement(2354..>3591)
                     /locus_tag="AC158Q_02550"
     CDS             complement(2354..>3591)
                     /locus_tag="AC158Q_02550"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008113665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HIR3338676.1"
                     /translation="EIRGHRRTYIGAIPGRIVQALKQAGSVNPVFMLDEIDKISVGIQ
                     GDPAAALLEVLDPEQNHTFVDHYVEVEYDLSDVMFVCTANTLNIPPALLDRMETIRLS
                     GYTEDEKVNIALKHLLPKLMKNHGLKPEELAISESAVRDIVRYYTREAGVRALERELS
                     KICRKVVKTLVSDGKERKVSVSARGLDKYLSVRRYTYGMAEKRNQIGQVSGLAWTEVG
                     GELLTIEAAIMAGKGKMTTTGKLGEVMQESVQAALSVVRARSRRLGIPSDFYQKNDIH
                     IHLPEGATPKDGPSAGIGICTAMVSALTGIPVRADVAMTGEITLRGEVLQIGGLKEKL
                     LAAHRGGIKTVLIPEENVKDLAEIPENVKNRLDIHPVKWIDTVLEMALERKPEPLTDE
                     EVASALPPVAAPAPAAVKH"
BASE COUNT          630 a         1167 c         1062 g          732 t
ORIGIN      
        1 ctcctgctcg atctggccgc gcacttcctc gaacggcttt acctgggcgg gctggaccga
       61 gaccgcccgt atgacgtgaa aaccatgccg ggtctccacc ggaggcgaga tttcgcccgg
      121 cttcaactgg tagagcgcgt tctcgaattc gggaatgtcc ttcatggtgc cacgggtgac
      181 ccgccccagg tcgccgccct gggccgccga accgggatcc tgggaatgct ttttcgcggc
      241 agcagcgaag ccggccgggt tcttccgcac ctcgttgtag atcgcgtccg cgcgctcgcg
      301 tgctttcttc ttcgcttcct cgcctgcccc cggctcgaca gcgatcagga tatgggctgc
      361 ctggcgtgat tcctcaacgc cgaactgcct gctgttggag tcgtagtatt tttttacctc
      421 ggccgggtcg gcctgcatct cctgcatcag cgaattgacc gataacgtga cgtactccac
      481 gcgcgcctgc tcgggtatcc ggaactcggc ggtattcgca tcgtagtatt gcttcgtggc
      541 gcccggatcg agcttgacct gggcaaggta gccctcgggc gcaaacgtgg catgactgac
      601 ttcgcgctgc tcgccggcca gccgggcaag ccgcccgacg accgtgcgcg gcgcgaagcc
      661 gttctcggcg tagccactgg agaattgctg aagaatcatg tcctggcgga tgcgcgcctc
      721 gaacgtggcc ggcgtcaaac cctcgttccg gaggaattgc tggtagatct caaaggagaa
      781 attcccggcc gggtcccgga acgtggccag atcgctgatg gtgctctgca actggctgtc
      841 ggataccgtg attcccgact tgagcgcccg atcgatcagc aagcggcgct ggatcatgct
      901 ctccagcgtg gcctggcgca gttcggggtt atccagcatc tccgggtcga tgcgcccctg
      961 caccgtgcgc tgtatcgatt gctggcgctc acgcagcgtg cgggcgaaat cctcctgcgt
     1021 gatggtatgg tctcccaccc gggcgacggc ctggccgcct tccatgccgc ggaaataggc
     1081 gtccacgccg aacagcgcga atggcggcac gatcaggatc aggaatacga tctggatcag
     1141 cttcttgtgc ttgtcgatga aatcaaacat gtcgcggctt tgtatcccgt taccttaaag
     1201 aaaaaggcga actgcgttcg cctttttctc gaatctggcg gagtggacgg gactcgaacc
     1261 cgcgacctct gccgtgacag ggcagcattc taaccaactg aactaccact ccgaattaca
     1321 gcgcagagtg caacatgatg aatgttgagc ggaaatcccg acccctcatt ctgcactcaa
     1381 cactctgcac tttacactgt aggtctggtg ggtgctgaga ggatcgaact cccgacattc
     1441 gccttgtaag ggcgacgctc taccagctga gctaagcacc cttaaaacga acgctttcgc
     1501 gccgctttaa gtgcgtccaa tcgagcggac cggatgcccc gctccatgca cgcaccggca
     1561 atccgttacc gccggtcccg gcgtcctccg aaatattttg gggcaggccg cgggcaaccc
     1621 cgcgcctacc ccaaattgga aaccggatcg caaccgggcg atcagtttac cgcatctttc
     1681 aatgccttac cagcactgaa cttgggcact tttgcggcct gtatgcgaat ttccgcgccg
     1741 gtgcgcgggt tgcgccccga acgcgcctgg cgcttgccga cccgaaaagt tccgaatccc
     1801 accagcgtga ccgactggcc gcgcttcagg gcgcccttga tcgcgtcgat cgcgccatcg
     1861 agcgcctttt ccgaggcgga cttggatatt cccgctgacc tggagacgac ttccacgaga
     1921 tcggctttgt tcatcatgcc cccccttctt caactgtccg tgacggtaac gacccccccg
     1981 ctgtccaaac cttcacgcga cgattgaaga aggccgagcg gaaaaacttt ttgtggttgg
     2041 tttgccggcg aacgcagctt ttgaaacgcg ctggtgactt tatagcaagc gcgaaaagcc
     2101 ggtgtcaagc aattgcaaaa atattttcac gggatcgtcg cacaaagttg cgcggaatgt
     2161 cacacgactc gacgggcgca ttgaagaagt aaaacgagga gcggcgaata agtgattgag
     2221 tgattgagga tagctccatt gggccggcgc gaacgaggca cggtccgctt ccaaaaaaag
     2281 aaagggcggc atcgccgccc tctcttcccg atcgaccggt caccgatcgc cggtcacgcg
     2341 cccgccttcg caactagtgc ttgacagcgg ccggcgccgg cgccgcaacc ggtggcaacg
     2401 ccgatgcgac ttcctcgtcg gtcagcggct cgggttttct ctcgagcgcc atttcaagca
     2461 cagtgtcgat ccacttgacc gggtggatgt cgagccggtt cttgacgttc tcgggaatct
     2521 cggcgaggtc cttaacgttc tcctcgggga tcagcacggt cttgatgccg ccgcggtgcg
     2581 ccgccagcaa cttctccttg agacccccga tctgcagcac ctcgcccctc agcgtgattt
     2641 cgccggtcat cgcaacatcc gcgcgcaccg ggatgccggt cagcgccgac accatcgcgg
     2701 tgcagatgcc gatgcccgcg ctcggcccgt ccttgggcgt cgcgccctcg ggcaggtgga
     2761 tgtggatgtc gtttttctgg tagaagtccg acgggatgcc cagtcggcgc gaccgggcgc
     2821 gcacgaccga cagcgcggcc tgcaccgact cctgcatcac ctcgcccagc ttgccggtcg
     2881 tggtcatctt gcccttgccg gccatgatcg ccgcctcgat ggtgaggagc tcgcccccca
     2941 cctcggtcca ggcgagcccc gacacctgcc cgatctggtt cctcttctcg gccatgccgt
     3001 aggtatagcg ccgcacgctc aggtacttgt cgaggccgcg cgcgctgacc gacaccttgc
     3061 gctccttgcc gtcgctcacc agggtcttga ccaccttgcg gcagatcttg gagagctccc
     3121 gctcgagcgc ccgcaccccg gcttcccgcg tgtaatagcg gacgatatcg cgcaccgcgc
     3181 tctcggatat ggcgagctcc tcgggcttca gcccatggtt cttcatgagc ttgggcagca
     3241 ggtgcttcag cgcgatgttg accttctcgt cctcggtgta gcccgagagc cggatcgtct
     3301 ccatgcggtc gagcagcgcc ggcgggatgt tgagcgtatt ggccgtgcac acgaacatca
     3361 cgtccgaaag gtcgtactcg acctcgacgt aatggtccac gaaagtgtgg ttctgctcgg
     3421 gatcgagcac ctccagcagc gccgcggccg ggtcgccctg gatgccgacg gagatcttgt
     3481 cgatctcgtc gagcatgaac accgggttga ccgagccggc ctgcttgagc gcctgcacga
     3541 tgcgtcctgg aatggcgccg atatacgtgc gccgatgccc tcggatttcc g
//
LOCUS       DBNGSG010000101         2521 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_33077, whole genome shotgun sequence.
ACCESSION   DBNGSG010000101 DBNGSG010000000
VERSION     DBNGSG010000101.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_33077"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2458)
                     /locus_tag="AC158Q_02555"
     CDS             complement(<1..2458)
                     /locus_tag="AC158Q_02555"
                     /inference="COORDINATES: protein motif:HMM:NF017930.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HIR3338677.1"
                     /translation="MAGAYDAGPESDAKWWAQALARQCRRALDELTFLAPWLLLPPAP
                     GRLSDFPDFGEIPTLRQLARLEAEWLPAIARRLDADATPGESGWLDQLRGAVTEASRR
                     AAERIATLERLALQAGELARMEYDFLYDKARHALAIGYNAGERRRDSSYYDLLASESR
                     LASFVAIAQGQLPQDSWFALGRLLTNTAGAPTLMSWSGSMFEYLMPLLVMPNYENTLL
                     DQTCKAAVKRQIAYGNQRDVPWGMSESGYNAVDVHLNYQYRAFGVPGLGLKRGLAEDL
                     VIAPYASALALMVAPEEACLNLQRLAAEGLEGRFGFYEAIDYTPPRQRRGESSSVVRS
                     YMAHHQGMSLLSLAYLLLGRPMQKRFESDPLFQATLLLLQERIPKVTAFHLHAAELSD
                     IRAIVSGPEMPVRVLASPDTPVPEVQLLSNGRYHVMVTNAGGGYSRWKDLAVTRWRED
                     RTRDNWGSFCYIRDVASGVFWSAAHQPALLRSPAYEAIFSEGRAEFRRSDHDFDAHTE
                     IVVSPEDDIELRRLHVTNRARTRRAIDVTSYAEVVLAPPAADALHPAFSNLFVQTEII
                     RERQAILCTRRPRSLEERPPWMFHLMAAHGADVEEISYETDRMRFIGRGRTVAAPRAM
                     SEPAALSGTEGSVLDPIVAIRYRITLDPEQSATIDMVSGIGETRDAALGLVTKYQDRR
                     LADRVFDLAWTHSSVTLRQINATESDAQLYGRLAGSVIYSNPSLRADAAVLGKNRRGQ
                     SSLWGYAISGDLPIVLLQIGDLANVELVRQLVQAHAYWRLKGLAVDMVIWNEDRAGYR
                     QVLQEQIMGLIAAGIEAHVMD"
BASE COUNT          421 a          837 c          842 g          421 t
ORIGIN      
        1 gatccatcac gtgcgcttcg atgcctgcgg caatcagccc catgatctgt tcctggagca
       61 cttgccggta accggcgcgg tcttcgttcc agatcaccat gtccacggcg agtcccttca
      121 ggcgccagta ggcgtgggcc tgcacgagct ggcgcaccag ctcgacattc gccaggtctc
      181 caatctgcag cagcacgatc ggcaggtcgc cggaaatggc gtagccccac aggctggatt
      241 gcccgcgccg attcttcccg agaacggctg cgtcggcgcg cagcgaggga ttggagtaga
      301 tgacggaacc ggcgaggcgt ccatagagtt gcgcgtcgga ctccgtggcg ttgatctgcc
      361 gcagggtcac cgagctatgg gtccacgcca gatcgaagac gcggtccgcc aggcgccgat
      421 cctggtattt cgtgaccagg cccaaggccg cgtcgcgggt ttcgccgatg ccggagacca
      481 tatcgatcgt tgccgattgc tccggatcga gcgttatgcg atagcggatc gcaacgatcg
      541 gatccagcac cgatccctcg gtgcccgaga gcgccgccgg ctcgctcatc gcacggggcg
      601 cggcgaccgt gcggccgcgg ccgatgaacc gcatgcgatc ggtctcgtag gaaatctcct
      661 cgacgtccgc cccgtgcgcg gccatcaggt gaaacatcca gggcgggcgc tcctcgaggg
      721 agcggggccg gcgcgtgcag aggatggcct gccgctcgcg gatgatctcg gtctggacga
      781 agagattgct gaacgccgga tgcagtgcgt ccgcggccgg gggcgcgaga acgacctccg
      841 cgtaactcgt gacgtcaatc gcccggcgtg tccgggcgcg gttggtgacg tggagccggc
      901 gcagctcgat gtcgtcttcc ggcgaaacca cgatctcggt atgcgcatcg aagtcgtggt
      961 cgctgcgccg gaattcggcg cgcccctccg agaaaatcgc ttcgtaagcc ggcgatcgca
     1021 ggagcgccgg ctgatgggcg gctgaccaga acaccccgct cgccacgtcg cggatgtaac
     1081 agaagctgcc ccagttgtcg cgggtcctgt cttcgcgcca gcgcgtgacg gcgaggtctt
     1141 tccagcggct gtagccgccg ccggcgttcg tgaccatcac gtggtatcgg ccgttcgata
     1201 gcagctgcac ctccggtacc ggggtgtcgg ggctcgcgag cacgcgcacc ggcatctcgg
     1261 ggccgctgac aatcgcgcga atgtcggaca attcggcggc gtgcagatgg aacgcggtca
     1321 ccttgggaat tcgctcctga agcagcagca gggtcgcctg gaacagcgga tccgactcga
     1381 atcgcttctg catcggacgg cccagcagca aataggccag ggagagcagg ctcatgccct
     1441 ggtggtgggc catgtaggac cggaccaccg agctcgattc cccccgccgt tgacgcggcg
     1501 gggtgtagtc gatggcctcg tagaagccga atcttccttc cagcccttca gcggcgagcc
     1561 gctgcagatt caggcacgcc tcctcgggcg ccaccatcag cgcgagcgcc gaagcatacg
     1621 gggcaatgac cagatcctcg gccagcccgc gcttgagccc caggccggga acgccgaagg
     1681 cgcggtactg gtagttgaga tgaacgtcga ccgcgttgta gccggattcc gacatgcccc
     1741 acggcacatc gcgctggttc ccgtacgcga tctgtcgctt cactgccgcc ttgcaggtct
     1801 ggtcgagcag ggtgttttca tagttcggca tcaccaggag cggcatcagg tactcgaaca
     1861 tcgagccgct ccaggacatg agcgtcggtg cgcccgcggt attggtcaga aggcgcccca
     1921 gagcaaacca gctatcctgc ggcagttgtc cctgggcgat cgccacgaaa ctggccaatc
     1981 tcgattccga ggccagcaga tcgtagtagc tcgagtcgcg ccggcgctca ccggcgttgt
     2041 agccgatcgc cagcgcatga cgtgccttgt cgtagaggaa gtcatactcc atccgggcga
     2101 gttctcccgc ctgcagtgcc aggcgttcga gggtcgcaat ccgctcagcg gcgcgccggc
     2161 ttgcttccgt gacggctcct cgcagttgat cgagccaacc gctttccccg ggcgtcgcgt
     2221 ccgcgtcgag ccggcgtgcg attgccggca gccactccgc ctcgagtctc gccagctggc
     2281 gcagcgtcgg gatctcgccg aagtcgggga agtcgctgag cctgccgggc gcaggcggca
     2341 gcaacagcca tggagcgaga aacgtcagct catcgagggc tctgcggcat tgccgggcca
     2401 gcgcctgtgc ccaccacttc gcatcgctct cgggaccggc gtcgtaggcg ccggccactc
     2461 ccgcagcgga cgccgcaagc cggtcaagcc aacgccaagc cgccgcgacg gtggcgggcc
     2521 g
//
LOCUS       DBNGSG010000102         4559 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3428576, whole genome shotgun sequence.
ACCESSION   DBNGSG010000102 DBNGSG010000000
VERSION     DBNGSG010000102.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4559)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3428576"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /gene="proB"
                     /locus_tag="AC158Q_02560"
     CDS             complement(<1..863)
                     /gene="proB"
                     /locus_tag="AC158Q_02560"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HIR3338678.1"
                     /translation="MASVLANAKRLIVKVGSSLVTDQGRGLDLAALSRWAEQISQLRQ
                     AGRQVVLVSSGAIAAGMQRLGWDRRPHAVHELQAAAAVGQMGLIQAYESCFREHQLLT
                     SQILLTHEDLADRKRYLNARSTLRTLLDLGVVPIINENDTVAIDEIRFGDNDTLAALV
                     TNLIEADALVILTDQAGLYDRDPRKDDTATLIAEARAGDPALEAMAGGTGSPIALGGM
                     LTKVLAAKRAAHSGAHTVIASGHEDNVLPRLARGERIGTQLVAERATLAARKQWLADH
                     LQVRGSLTLDSG"
     gene            complement(869..1945)
                     /gene="cgtA"
                     /locus_tag="AC158Q_02565"
     CDS             complement(869..1945)
                     /gene="cgtA"
                     /locus_tag="AC158Q_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010091263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HIR3338679.1"
                     /translation="MKFVDEAIIEVHAGKGGDGAASFRREKFVPRGGPDGGDGGRGGG
                     IYAVADRNINTLIDYRFARIHRARDGENGRGADQYGKSASDVLLRVPVGTVVSDLETG
                     ETIADLGHDGERALLAAGGAGGIGNLHFKSSTNRAPRQFTRGGEGEHRRLKLELKLLA
                     DVGLLGMPNAGKSTLIRALSAARPKVADYPFTTLAPSLGVVRADMNRSFVLADIPGLI
                     EGAAEGAGLGHQFLRHLARTRLLLHLVDIAPLDTGTDTAREAKAIVNELRKYDDTLYR
                     KPRWVVVNKIDMIPVEERARTVRRFLKDLGWKGKSFIISALTGEGCRELSFAVMEHLE
                     RDVESGDQNTAKAKPKRRKTTPAS"
     gene            complement(2013..2270)
                     /gene="rpmA"
                     /locus_tag="AC158Q_02570"
     CDS             complement(2013..2270)
                     /gene="rpmA"
                     /locus_tag="AC158Q_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HIR3338680.1"
                     /translation="MAHKKAGGSSRNGRDSQSKRLGVKRYGGELVSAGSIIVRQRGTQ
                     VHPGANVGMGRDHTLFAKVDGRVQFAQKGPDNHKRVSVVPA"
     gene            complement(2385..2693)
                     /gene="rplU"
                     /locus_tag="AC158Q_02575"
     CDS             complement(2385..2693)
                     /gene="rplU"
                     /locus_tag="AC158Q_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HIR3338681.1"
                     /translation="MYAVVKTGGKQYRVSAGEKLRIEQITAEIGQEIVLDQVLVVADG
                     DALKMGTPLLAGAQVKAKVLAHGRGEKVHIFKMRRRKHYRKSQGHRQNYTEIEILGIA
                     "
     gene            2915..3856
                     /locus_tag="AC158Q_02580"
     CDS             2915..3856
                     /locus_tag="AC158Q_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR3338682.1"
                     /translation="MEADMRAVDGVIRTRLNSGVLLIRQVAEHIIGGGGKRLRPMLLL
                     LSAGAFGYRGSHRHELAAVVEFIHTATLLHDDVVDESGLRRGQATSNALFGNATAVLV
                     GDFVYSRAFQMMVETDNLRVLRVLADATNVIAEGEVMQLMNCHNPDIEEDGYLEVIRC
                     KTAKLFEAATRLGAILGAAPRAQEDAIADYGTRLGTAFQLIDDVLDYSGEQALIGKNL
                     GDDLAEGKPTLPLIYAIKHGTQRQARLVRHAIEHGGRDELGGVIEAIHSTGALDYARE
                     QARQESRGACEALAGLPDSRERDYLLQLADFAVTRSY"
     gene            3917..3993
                     /locus_tag="AC158Q_02585"
     tRNA            3917..3993
                     /locus_tag="AC158Q_02585"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Pro,seq:cgg)
     gene            4030..4266
                     /locus_tag="AC158Q_02590"
     CDS             4030..4266
                     /locus_tag="AC158Q_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HIR3338683.1"
                     /translation="MAKIILLVLGLLAVYWILKSYRRRVDRSNSAPPAAAGEDMVQCA
                     HCGVHLPRSESITTQGHFFCTAEHQRAHQVGRRP"
     gene            4266..>4559
                     /locus_tag="AC158Q_02595"
     CDS             4266..>4559
                     /locus_tag="AC158Q_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338684.1"
                     /translation="MSSAKAERPALWEVDASAPPGEHPEPFWRSLFYFNAYRLFVALL
                     LLASVAIWGPNLWIGSRDLKLFVVANISYLVFGVACFVLISARRRFNLQLTLQV"
BASE COUNT          697 a         1452 c         1514 g          896 t
ORIGIN      
        1 ccggaatcca gcgtcaggct gccgcggacc tggaggtggt cggcgagcca ctgtttgcgt
       61 gccgcgagcg tcgcccgctc cgcgacgagc tgcgtgccga tccgctcgcc gcgcgcgagc
      121 cgcggcagca cgttgtcttc atgtcccgat gcgatcacgg tatgggcgcc gctgtgcgcg
      181 gcgcgcttcg cggcaagcac cttggtcagc atgcctccga gcgcaatcgg gcttccggtg
      241 ccgcccgcca tcgcctccag cgccgggtcg cccgcgcggg cttccgcgat cagggtcgcg
      301 gtgtcgtcct tgcgcgggtc gcggtcgtag aggccggcct ggtcggtgag gatcaccaat
      361 gcgtcggcct cgatcaggtt ggtgaccagg gccgccagcg tgtcgttgtc gccgaagcgg
      421 atctcgtcga tggcgacagt gtcgttttca ttgatgatgg ggacgacacc caggtcgagc
      481 agcgtacgca gggtggagcg ggcattgaga tagcgcttgc ggtccgccag gtcctcgtgc
      541 gtgagcagga tctgcgacgt cagcagctgg tgctcgcgga agcacgactc gtaggcctga
      601 atcaggccca tctggcctac tgcggccgcc gcctgcagtt cgtgcaccgc gtgcgggcgc
      661 ctgtcccagc cgaggcgctg catgcctgcg gcgatggcgc cgctcgaaac cagcacgacc
      721 tggcggccgg cctgccgcaa ttgcgagatt tgctccgccc agcgcgagag cgcggcaagg
      781 tcgagcccgc ggccctggtc ggtgaccagg ctcgagccga ctttcacgat cagtcgcttg
      841 gcgttggcga ggacggaggc catgtgggtc aggatgccgg tgttgttttc ctgcgtttgg
      901 gcttggcctt ggccgtattc tggtccccgg attccacgtc ccgctccaga tgctccatga
      961 ccgcgaaact gagctcgcgg cagccttcgc cggtgagcgc ggagatgata aagcttttgc
     1021 ctttccaccc caagtccttg agaaatcgcc tgaccgtgcg cgcgcgctct tcgacgggaa
     1081 tcatgtcgat cttgttgacc acgacccagc gcggcttgcg gtagagcgta tcgtcgtatt
     1141 tccgcagctc gttgacgatc gcttttgcct cgcgcgcggt gtcggtgccg gtgtctagcg
     1201 gggcgatgtc caccaggtgc agcaggagcc tcgtgcgcgc caggtggcgc aggaactggt
     1261 gtccaagacc cgctccctcg gccgcacctt cgatcaggcc cgggatgtcg gcgagaacga
     1321 aactgcggtt catgtccgcg cgcaccacgc cgaggctggg cgcaagcgtg gtgaaggggt
     1381 agtcggccac cttggggcgc gcggccgaga gcgcccggat cagggtggac ttgccggcat
     1441 tgggcatgcc gagcaggccc acgtctgcga gcagcttcag ctcgagtttc aaccggcgat
     1501 gctcgccttc gcccccgcgc gtgaactggc gcggcgcgcg gttggtgctc gacttgaagt
     1561 gcaggttgcc gatgccgccg gcaccgcccg ccgccagcag cgcgcgctcg ccgtcgtgcc
     1621 cgagatcggc gatcgtttcg cccgtttcca ggtcgctcac caccgttccg accggcacgc
     1681 gcagcaacac gtcggaagcc gatttgccgt actggtcggc gccgcggcca ttttcgccgt
     1741 cccgggcgcg gtggatgcgc gcgaagcggt aatcgatcag cgtattgatg ttgcggtcgg
     1801 ccactgcata aatgccgccg ccgcgcccgc cgtcgccgcc atcggggccg ccgcgcggaa
     1861 cgaatttttc gcgccgaaaa ctggctgcgc catcgccgcc cttgccggca tggacctcga
     1921 tgatggcttc gtcgacaaac ttcattgatt atccgccatg aaaacaaaaa gccctgtcac
     1981 gtgacagggc tttgggcggg cggcgcgtcc gctcaggcgg gaacgacgct cacccgcttg
     2041 tgattgtccg gtcccttttg cgcgaactga acgcgtccgt ccacctttgc aaagagcgtg
     2101 tgatcgcgcc ccatgccgac gttcgcgccg gggtgcacct gggtgccgcg ctggcgcacg
     2161 atgatcgatc cggcgctcac cagctccccg ccgtagcgct tcacgccgag acgcttggat
     2221 tgggagtcgc gcccgtttct cgaactgccg cctgcttttt tatgtgccat gacctgctcc
     2281 tagactgatg tcggggtgca cgaggggagt atcccctcat tcgttccaat ttttctgttc
     2341 gccaaggtag gggtgcacga ggggagtatc ccctcgttct ttacctatgc gatgccgaga
     2401 atctcgatct cggtgtaatt ctggcgatgt ccctggctct tgcgataatg cttgcggcgc
     2461 cgcatcttga agatgtgcac cttctcgccg cggccgtggg ccagcacctt ggccttgacc
     2521 tgggcgcccg ccaggagcgg cgtgcccatt ttgagtgcgt cgccatcggc gaccaccaga
     2581 acttgatcca gcacgatttc ctgcccgatt tcggcggtta tctgctcgat cctgagtttt
     2641 tccccggcac tgacccggta ctgcttgccg ccggtcttga ccaccgcgta catatcgccg
     2701 cccgcctgtt gattgctcgg attgaagagc cgcgcattct acaaaaacgg cgccgtttag
     2761 tcaaagagaa tccatgcggg caagagccgg ctccggtttt gccatcaagg ggttagagcg
     2821 gcggtccggg ccggagcccg ggcggttttt actggtatca tcccccctct tttcatgggg
     2881 cgaagcggtg acgctggagg cgatacgcgg gttcatggag gccgatatgc gcgccgtgga
     2941 cggtgtgatt cgtacccggc tcaattccgg cgtgctgctg atccgccagg tggcggaaca
     3001 cattatcggc ggcggcggca agcgcctgcg ccccatgctg ctgttgctct ccgcgggcgc
     3061 tttcggctac cgcggttcgc accgccacga gcttgccgcg gtggtcgagt tcatccatac
     3121 cgcgacgctg ctgcacgacg acgtcgtcga cgaatccggt ttgcggcgcg gccaggcgac
     3181 atccaatgcg ctattcggca atgccaccgc ggtgctggtg ggcgatttcg tctactcgcg
     3241 cgcgttccag atgatggtgg aaacggacaa cctccgtgtg ctgcgggtgc tggcggacgc
     3301 gaccaacgtg attgccgagg gcgaggtgat gcagttgatg aactgccata atcccgacat
     3361 cgaggaggat ggctatctcg aagtgatccg ctgcaagacg gcgaaactgt tcgaggcggc
     3421 cacgcgtctc ggtgctatcc tgggcgccgc gccgagagcg caggaggacg cgatcgccga
     3481 ttatggcacc cgcctcggca cggcattcca gctgatagac gacgtgctcg actactccgg
     3541 cgagcaggcg ttgatcggca agaacctggg cgacgatcta gcggagggca agcccacgct
     3601 gccgctcatc tacgcaatca agcatggcac gcagcggcag gcgcgcctgg tccggcacgc
     3661 gatcgagcat ggcggtcgcg atgaactggg cggggtgatc gaggcgatcc attccaccgg
     3721 cgcgctcgac tatgcgcgtg agcaggcgcg gcaagaatcg cgcggcgcct gcgaggcgct
     3781 cgcgggtctg ccggattcaa gagagcggga ttatttgcta caattggcgg acttcgcagt
     3841 gacacggagt tactgacgga gttcgaacat tatgaacgag ggcacggagc agcgtgccct
     3901 tgttgtttct acaactcggg gtgtagcgca gcctggtagc gcactgcgtt cgggacgcag
     3961 gtgtcggagg ttcaaatcct ctcaccccga ccattttcct gatagcgtcg cccggtagcg
     4021 gaggtttgat tggccaagat catcctgctg gtgctcgggc tgctcgccgt ttactggata
     4081 ctgaagagct atcgtcggcg cgttgaccgc agcaattcgg cgccgccggc cgcggcgggg
     4141 gaagacatgg tccagtgcgc ccactgcggc gtgcatttgc cgcgcagcga gagcatcacc
     4201 acgcagggcc attttttctg taccgccgag catcagcggg cgcaccaggt ggggcggcgg
     4261 ccgtgatgag ctcggccaag gccgaacgcc ccgcgctgtg ggaggttgac gcatccgcgc
     4321 cccccggcga gcatcccgag ccgttctggc gctcgctgtt ctatttcaac gcttaccgcc
     4381 tgttcgtcgc gctgctgctg ctcgcgagcg tggcgatctg gggaccgaac ctctggatcg
     4441 gctcgcgcga ccttaagctg ttcgtggttg ccaacatctc ctacctggtg ttcggcgtcg
     4501 cctgcttcgt gctgatcagc gcgcggcggc gtttcaacct gcagctgacg cttcaggtg
//
LOCUS       DBNGSG010000103         2517 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3428916, whole genome shotgun sequence.
ACCESSION   DBNGSG010000103 DBNGSG010000000
VERSION     DBNGSG010000103.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3428916"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(262..1947)
                     /locus_tag="AC158Q_02600"
     CDS             complement(262..1947)
                     /locus_tag="AC158Q_02600"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR3338685.1"
                     /translation="MTKVKCGVAAVQALRASGVSKVFGLMGSSTLEIYDALYDAKDMQ
                     YIGVRHENCGMHMADAYGRVTQTPGLFICGQAGPGSANMVLGLMAAKLAYSPVVAITG
                     LASTEHLGRDAFQEIDQNTLFLPVAKRVMTVTRPERIPEFILEAFRIAGSGRFGPVVV
                     NIPRDLLNHSIDVAIPAAGSRAPMAGGAVDRATLERIKAMLLAAKAPVIHAGAGIKWG
                     RGTAGLLKLAEKLQIPFTNSAGHSDVAPADHPLYAGGSGARGNPVASTLMREADFILA
                     LGTRLGFNTTGYKYDNLSPSAKIVQVDIDAVAVGRYFPVEMGIVGDAGAVAGALADIM
                     DAISAAAAPWKARNEKFVSERQALWRRREESGSRTGKPLHPDVIYAELRKVAPANSLF
                     TLDAGTTGLQATDQLPFRAVPSLITPLDCACVGLSYAAGLGAKVAAPERTVITLMGDG
                     GFGMTMGEMMTAVESRINSIAIVMDNGTWGSEIAYQRDFYNGRTIGAHVHSPRFDEVM
                     KLCGGNGYFVTGAGGTADAVNQAMKGGKPAVIHVKVDPEATVSFRTDALVKRS"
     gene            complement(2170..>2517)
                     /locus_tag="AC158Q_02605"
     CDS             complement(2170..>2517)
                     /locus_tag="AC158Q_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338686.1"
                     /translation="QPIQVRRLVYDEAKYSEVVSGVKSRASRPSVLEVRDHFAGYFDE
                     LGLWVQFRDGTSDQEVIEVGNELLTYLNDVLPRNNESFRWILSVERGRDTLDVIVPGD
                     TPRDLNNKLAPIK"
BASE COUNT          464 a          834 c          749 g          470 t
ORIGIN      
        1 actggacaac gcgcaagatc agcggcgagc atgccaccag ctcgtcgacc gtcatcccgc
       61 tgcatccaag gaacaagcat cttcactagc ggcgggctcc gccgcgagag atgagggttc
      121 acactccctg ggcgtcactg atttcctgtt atcgtttcgc gtcattcccg cgtgttgaac
      181 agcgacgatg tcgcctgctc gaacacgcgg agttaaaccg gcgccttgcg tccggattta
      241 acggcacttg gcggtaaatt tctaactacg ttttaccaag gcgtcggtgc ggaagctgac
      301 cgtcgcttcc ggatccacct tgacgtggat caccgcgggc ttgccgcctt tcattgcctg
      361 gttcaccgcg tcggcggtgc cgccggcgcc ggtcacgaaa tagccgttgc cgccgcagag
      421 cttcatcacc tcgtcgaaac gcggcgagtg cacgtgggcg cctatggtgc gcccattata
      481 aaagtcgcgc tggtaggcga tctccgagcc ccaggtgccg ttgtccatca cgatggcgat
      541 gctgttgatg cggctctcca ccgccgtcat catctcgccc atggtcatgc cgaaaccgcc
      601 gtcgcccatc agggtaatca cggtgcgctc cggcgccgcg accttcgcgc ccagccccgc
      661 cgcgtaggac aagccgacgc aggcgcaatc gagcggcgtg atcagggatg gcacggcgcg
      721 gaacggcagc tggtcggtcg cctgcaggcc ggtggtgccc gcgtccaggg tgaaaagcga
      781 gttggccggc gccaccttgc gcagctcggc gtagatcacg tccgggtgca gcggcttgcc
      841 tgtgcgtgat cccgattcct cgcgccgccg ccacagcgcc tggcgctcgc tcacgaattt
      901 ctcgttgcgc gccttccacg gcgcggcggc cgcggaaatc gcgtccatga tgtccgcgag
      961 tgcgcctgca acggcgccgg catcgccgac gatacccatc tcgaccggaa agtagcggcc
     1021 gacggcgacg gcatcgatgt ccacctgcac gatcttcgcc gagggcgaaa ggttgtcgta
     1081 cttgtagccg gtggtgttga agcccagccg cgtgccgagc gcgagaatga agtccgcctc
     1141 gcgcatgagc gtgctcgcaa ccggattgcc cctggcgccg gacccgcccg catatagcgg
     1201 atggtcggcc ggagcgacat cgctgtgccc tgcggaattg gtgaacggaa tctgcagctt
     1261 ttccgcgagc ttcaacaggc ccgccgtgcc gcgcccccat ttgatgccgg cgccggcatg
     1321 aatcaccggc gctttcgcgg cgagcagcat cgccttgatg cgctccagcg tggcgcggtc
     1381 aaccgccccg cccgccatcg gtgcccggct tcccgccgca ggaatggcga cgtcgatact
     1441 gtggttgagc aggtcacgcg gaatattcac caccaccggc ccgaagcggc cggagccggc
     1501 gatgcggaat gcttcgagga tgaattcggg gatgcgctcg ggacgcgtca cggtcatcac
     1561 gcgcttcgcc accggcagaa acagcgtgtt ctggtcgatc tcctggaacg catcgcgccc
     1621 gaggtgctcg gtcgaggcca gcccggtgat ggcgacgacc ggcgagtacg ccagcttcgc
     1681 cgccatgagc cccagcacca tgttcgccga gcccgggccg gcctggccgc agatgaatag
     1741 ccccggcgtc tgcgtcacgc gcccgtaggc atccgccatg tgcatgccgc agttctcgtg
     1801 gcgcacgccg atgtattgca tgtctttcgc gtcgtagagc gcgtcgtaga tctcgagcgt
     1861 gcttgaaccc atcagcccga aaaccttgct cacccccgac gcgcgcagcg cctgcacggc
     1921 ggcaacgccg catttcacct tcgtcatcgt taaaacccca aattaaccgc caagacgcca
     1981 aggcgccaag aaaatcaata caaataattg aactgccaag caccgttaaa gccggacgcc
     2041 acgcgccggt ttaactctgc gcgttcgaac ggacgaatca atcgccgttc aacgcgcaga
     2101 acctccaacg tgtccgcgtg aagtgcgcga cagcgtcact ttcgacgcgg gggttagacg
     2161 gcgacctgtt catttgatcg gtgccaactt gttattgagg tcccgtggtg tgtcgccagg
     2221 aactattaca tccaaagtat cccgtccgcg ctccacggac agtatccaac ggaacgactc
     2281 gttgttacgg ggaagcacgt cattcagata tgtcagcaac tcgtttccaa cttcaatgac
     2341 ttcttgatcg gaagtgccgt ctcggaactg aacccacagc cccagttcat cgaaatatcc
     2401 cgcgaaatga tcgcgaacct caaggacgct cggtcgcgag gcccgggact ttacgcccga
     2461 tacgacctcg ctatatttgg cttcgtcgta cacaagccgt ctaacttgta ttggttg
//
LOCUS       DBNGSG010000104         5963 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2410985, whole genome shotgun sequence.
ACCESSION   DBNGSG010000104 DBNGSG010000000
VERSION     DBNGSG010000104.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5963)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5963)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5963
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2410985"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="AC158Q_02610"
     CDS             complement(<1..284)
                     /locus_tag="AC158Q_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338687.1"
                     /translation="MNSDRGAVRPVRENLVTFCKREGITKAKGKRQKEKGKRKVEGEQ
                     GVCRSLSPFHFSLSPSTKKGGLGRPFLLPFAGYAAASDSRFSETCMIASPA"
     gene            342..869
                     /locus_tag="AC158Q_02615"
     CDS             342..869
                     /locus_tag="AC158Q_02615"
                     /inference="COORDINATES: protein motif:HMM:NF019430.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HIR3338688.1"
                     /translation="MNKLILTTLVSAGLAAATPLSMAQTAGAEGGPQARQSAQRQHHE
                     QRAFRSPSDRVEARLAYLKTALKITDAQLPQWNAYAETRRKQAREATERMQKFRAQMG
                     ERKPGTQPTAVERLERRQAMLAVASTRLNETLAATKPLYAALSPDQQKIADEVLAPRG
                     RGGFQHRGGHGRGRA"
     gene            complement(1199..2662)
                     /locus_tag="AC158Q_02620"
     CDS             complement(1199..2662)
                     /locus_tag="AC158Q_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338689.1"
                     /translation="MTDMNNWMLWPDNTALSVLFVLLAAMVVLYAARAPMHGLIRIAG
                     HALCGPMRLGARWLAAAAGDMKRRNRAVLLAQGRREAGQRVEREFERIGILVTRDLQG
                     YPALQRKLLEEITRVEEDYQKCGEVMPPPPDWVDAVAAVAQIKTGGGEVVRKILTEIR
                     DSIERIHDRAITEYRRAYQTRHGILAGFMPYWRSLDKTLQQVDTKMAEVQESAATVDR
                     QMGKYQEIVAGTDEAESALTNSSFTQFTISVIVLWIALGGALINFKLIALPMSEMVGA
                     GDYLTATLRTSEVAALVIILLEASMGLFLMESMRITHLFPRIANLNDRTRRRMIWISL
                     ALLVMLAGIEATLALMRDMLIADKQALLQSLATAPAAASAEGWIARIPTAGQMILGFI
                     LPFALAFVAIPLESFIHSTRTLLGALAVDFVYALGFMLRVACSLVRLAIRALLRFYDL
                     LIVLPLMAERRLKSLRGREPAAKARPLDAADKYVSPF"
     gene            complement(3020..4081)
                     /locus_tag="AC158Q_02625"
     CDS             complement(3020..4081)
                     /locus_tag="AC158Q_02625"
                     /inference="COORDINATES: protein motif:HMM:NF021778.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HIR3338690.1"
                     /translation="MFAYVGCFTTEKRKAKGRGVAVFRIDPASGAWAFVEACDVVPNP
                     SYICLDRTQRFLYSAHGDSDEVCSYARDPQSGKLRFLNKQKTEGDNSSTVEVDAGNRW
                     VLLSTGPGVALFPRNADGSLGPYTDLIVPEGEPGPLRGEQEHSHPHQASFDLTGRFVV
                     VPDKGLDKVLVYRFDAGRGKLTPADALRSRYASVPRHVAFHPGRPYAYVVMERDSTVF
                     ACHWDTGAGTLRPFQRITTLPTDYVGDNMAAEIAILSSGKFLYASNRGHESIAIYAVD
                     AATGKLEPAGWEPVQGRKPRFFCLDPDATHLYAANQDSHAIVEFRINHEIGKLTPTGQ
                     VIETGSPTCVVFATRQGGH"
     gene            complement(4247..4690)
                     /locus_tag="AC158Q_02630"
     CDS             complement(4247..4690)
                     /locus_tag="AC158Q_02630"
                     /EC_number="5.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HIR3338691.1"
                     /translation="MSIDDNMALVRRLYEEGLNRRDAAAAAAFYAEDAKNHGRTVGRA
                     GMQRVFESLFSVFPDFHYRIEEATAEDDRVVCKVTMTCTHLGLPTLPEVFSGMLKGVA
                     PTGRRVEVLHFHSFRIRGGQIAEHAAVRDDLGMLMQLGLVKRPAG"
     gene            complement(4783..5118)
                     /locus_tag="AC158Q_02635"
     CDS             complement(4783..5118)
                     /locus_tag="AC158Q_02635"
                     /inference="COORDINATES: protein motif:HMM:NF023171.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3301 domain-containing protein"
                     /protein_id="HIR3338692.1"
                     /translation="MSRRHYLFAFMEVLGILALAAIAWFWLDSLKAREAAVRAAREIC
                     IAEGMQFLDDTVAISSLKPVRDADGSLTLLRAYGFEFSDTGNNRLKGSVVMQGHRVAL
                     LNVGLRVLH"
     gene            complement(5161..>5963)
                     /locus_tag="AC158Q_02640"
     CDS             complement(5161..>5963)
                     /locus_tag="AC158Q_02640"
                     /inference="COORDINATES: protein motif:HMM:NF014523.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HIR3338693.1"
                     /translation="PVIAAVVAGYLVFDRVRNLGPEITIRFKDGGGVKIGQTPVKYRG
                     VPVGQVTAVELSEDRQWVAVKARLQRSAASIAQQGSVFWIVRPEVGIGDITGLSTVLT
                     GPEIQALPGTGAPAADFEGLERAPTALEVKGLKIVLRAPRVGSLRSNSPVYYRGVEVG
                     VVQSADLSANAATVDIHVLIRQRYADLVRGGSVFWNVSGVSVSGGLFRGVELKLESMR
                     SLAAGGIAFATPDGKSARAKEGTVFVLHDAPRKEWIAWAPRINIPPEK"
BASE COUNT         1104 a         1949 c         1832 g         1078 t
ORIGIN      
        1 gcgggcgagg cgatcatgca tgtttcggag aaccgggaat ccgaggccgc ggcgtagccg
       61 gcgaacggca acaaaaaagg gcggccgagg ccgccctttt ttgttgaagg ggaaagggaa
      121 aagtgaaaag gagaaagcga tctgcaaacc ccctgttcgc cttccacctt ccttttccct
      181 ttttcctttt gccttttgcc ttttgccttg gtaatcccct cccgcttaca aaacgttaca
      241 agattttccc gaacggggcg gacggcaccc cggtctgaat tcatgagttc ctcgcaaccc
      301 ggggggagcg gatgattccc gaacgaccaa ggagagaaac catgaacaag ttgatcctga
      361 cgactcttgt atccgccggt ctcgcggcgg ccaccccgct gtcgatggcc cagacggcag
      421 gcgcggaagg cgggccgcaa gcccggcagt ccgcgcaacg gcagcaccac gagcagcgcg
      481 cgttccgctc gcccagcgac cgggtcgagg cgcggctcgc ctacctgaag accgccctga
      541 agatcaccga tgcccagctg ccgcaatgga atgcctacgc cgagacccgg cgcaagcagg
      601 cgcgcgaagc gaccgagcgc atgcagaagt tccgggcgca gatgggcgag cgcaagccgg
      661 ggacccagcc cacggcggtc gagcgtcttg agcgcaggca ggccatgctc gctgtcgctt
      721 cgacgcggtt gaatgaaacc ctggcggcca cgaaaccgct ctacgcggcg ctctcgcccg
      781 atcagcaaaa aatcgccgac gaggtgcttg cgccccgcgg gcgcggcggg ttccagcacc
      841 gcggaggcca cggtcgcgga agggcgtgat ccgcaacaaa aagggcggcc gagaccgccc
      901 tttttactga ggtgaaagtc gaaaaagcaa gatatcgtca ttcccgcgaa ggcgggaatc
      961 cagggtcatc aatatgttac tgggtccccg ccttcgcgag gacgacttag cagaagttgc
     1021 aggcagtgtc gtttccgcct tttgcctttt gccttttgcc ttgtgcctta actcctcatc
     1081 cctccgccct catccctcat ccctcattcc tcatccctca tctgtcgccc tagctcctgg
     1141 cgaaaatggc gtcgagccgc tccaggtcgt tgatcacctt ccactcgttc ggcctccgtc
     1201 aaaagggact gacgtacttg tcggcggcat ccagcgggcg cgctttcgcc gccggctcgc
     1261 ggccgcgcag cgatttcagc cgccgctcgg ccatcagcgg cagcacgatc agcagatcgt
     1321 agaagcgcag cagcgcgcgg atcgccagcc gcaccaggct gcaggcaacg cgcagcatga
     1381 atccgagtgc gtagacgaaa tcgaccgcca gcgccccgag gagcgtccgc gtcgagtgaa
     1441 tgaaggattc gagcgggatc gccacgaagg ccagcgcaaa cggcaggatg aagcccagta
     1501 tcatctggcc ggccgtcggg atgcgcgcga tccagccttc ggcggacgcc gccgccggcg
     1561 ccgtggcgag cgattgcagc agggcctgct tgtcggcgat cagcatgtcc cgcatcagcg
     1621 ccagggtcgc ctcgatcccg gccagcatca ccagcagcgc gagcgatatc cagatcatgc
     1681 ggcggcgggt gcggtcgttc aggttggcga tgcgcgggaa gagatgcgtg atgcgcatcg
     1741 attccatcag gaacaggccc atcgacgcct cgagcaggat gatgacgagc gcggccacct
     1801 cggaagtgcg cagcgtcgcg gtgaggtagt cgcccgcgcc gaccatttcc gacatcggca
     1861 gcgcgatcag cttgaaattg atgagcgcgc cgcccagcgc gatccacagg acgatgacag
     1921 aaatcgtgaa ctgggtgaag gaggagttgg tcagggcgga ctccgcctcg tcggtcccgg
     1981 ccacgatttc ctggtatttc cccatctgcc tgtccaccgt ggccgcgctt tcctgcacct
     2041 ccgccatctt cgtgtcgacc tgctgcaggg tcttgtcgag agaccgccag tacggcatga
     2101 atcccgccag gatgccgtgc cgtgtctggt aggcgcggcg atactcggtt atggccctgt
     2161 cgtgaatccg ttcgatcgag tcgcggattt cagtcaggat tttccgcacc acttcaccgc
     2221 cgccggtctt gatctgcgcc accgcggcga ccgcgtcgac ccagtcggga ggcggcggca
     2281 tcacctcgcc gcatttctgg tagtcctcct ccacccgcgt gatttcctcg aggagcttgc
     2341 gctgcagggc gggatagccc tgcagatcgc gcgtgaccag gatgcctatg cgctcgaatt
     2401 cacgctcgac gcgctggccc gcttccctgc ggccctgcgc aagcagaacc gcgcggttgc
     2461 gccgcttcat atcccccgcc gcggcggcga gccagcgcgc gccgagacgc atcggcccgc
     2521 agagcgcatg gccggcgatg cggatgaggc cgtgcatcgg cgcgcgcgcc gcgtagagca
     2581 ccaccatcgc ggccagcaaa acgaacagca cggataacgc ggtattgtcc ggccatagca
     2641 tccagttatt catgtccgtc atggcgacct ccttccactt cccggctgtc ttcgctgctc
     2701 attacgatag atcaaccgaa gaataatttc ctgcaaaatt tgagatcggc aagacttatg
     2761 ccgcaaaatg aaacgcggcg cggggaaaat acaccgaata gtgatgcgaa attgagacac
     2821 tcgattttga aaatgcggcg aggcggattg tcgctgatca acgacaaatt ttattgaacc
     2881 gggaaaacac cgggataaaa caggaaggcg acagacagga ttaagaggac ttacgagaac
     2941 tttgaagtca ggcttattct ctgattcgct acaacagcgt gggtcgtgat tcgactcctt
     3001 ccgccttctg ccctgggttt caatgtccgc cctgccgcgt ggcgaaaacg acgcaagtcg
     3061 ggctgccggt ttcgatcacc tgccccgtcg gcgtgagctt gccaatttcg tggttgatcc
     3121 tgaactccac gatggcgtgg ctatcctggt ttgccgcgta aagatgcgtc gcgtccggat
     3181 cgaggcagaa gaagcgcggc ttcctgccct gcaccggttc ccagccggcg ggctcgagct
     3241 tgcccgtcgc tgcgtccact gcatagatcg cgatgctctc gtgcccgcgg ttggaagcgt
     3301 agaggaactt ccccgaggac aggatggcga tctcggccgc catgttgtcg ccgacgtaat
     3361 cggtcggcag cgtcgtgatg cgctggaaag gcctcagcgt gccggcgccg gtatcccagt
     3421 gacaggcgaa aacggtggag tcccgctcca tcaccacgta ggcgtacggc cggcccgggt
     3481 gaaaagccac gtggcgcggg acggacgcat aacgcgacct cagggcatcg gcgggcgtca
     3541 gcttgccgcg cccggcgtcg aagcggtaca ccagcacctt gtccagcccc ttgtcgggca
     3601 ccaccacgaa ccggccggtc aggtcgaacg atgcctggtg aggatgcgaa tgctcctgct
     3661 ctccgcggag cgggcccggc tcgccttccg gaacgatgag gtcggtatag gggcccagcg
     3721 acccgtcggc gtttctcggg aacagcgcca cgccaggccc ggtggacagc aatacccagc
     3781 ggttgcccgc gtccacttcg acggtcgagg agttgtcccc ctccgtcttc tgcttgttca
     3841 ggaaccggag cttgccgctt tgcgggtcgc gcgcgtagct gcacacttca tcgctgtcgc
     3901 cgtgcgccga atagagaaac cgctgcgtgc ggtcgaggca gatgtacgac gggttcggga
     3961 caacgtcgca ggcctcgaca aacgcccacg cgcccgacgc cgggtcgatc ctgaataccg
     4021 cgacacccct gcccttggct ttccgctttt cggtggtgaa acacccgacg tacgcgaaca
     4081 acgggcgctg actggattgt gattccatgc ggtgacggca ggtaaccgga aatgtctcaa
     4141 ttgatctgac tacttggggc tgcttaaggg aatagtcgaa agtgatagag gtgccctagc
     4201 tgtcttaccc ctgcactttt ccctttttcc ttttcccttt tacctttcag ccagccggcc
     4261 gcttcaccaa cccgagttgc atcaacatcc cgagatcgtc gcgcacggcg gcgtgttccg
     4321 cgatctgccc gccgcgaatg cgaaagctgt ggaaatgaag cacctcgacg cgccttccgg
     4381 tgggcgcaac gcccttcagc atgccgctga agacctcggg taacgtgggc agtccgagat
     4441 gggtgcacgt catggtgacc ttgcacacca cgcggtcgtc ctcggcggtc gcctcttcga
     4501 tgcggtagtg gaaatcggga aatacgctga agagggattc gaacacccgc tgcattcccg
     4561 cgcgaccgac tgtgcggcca tgatttttcg catcttcggc gtagaacgcc gccgccgccg
     4621 cggcatcgcg gcggttcaag ccctcctcgt agagccgccg caccagggcc atgttgtcat
     4681 cgatcgacat aactgaaggg aaaagtaaat aggaaaaagg gattctagcg ccgtgggcct
     4741 tgtgtccagc tccttttccc ttttcccttt ccccttttcc ctttaatgga ggacgcggag
     4801 cccgacattg agcagcgcga cccggtgccc ctgcatgacc acgctgccct tcaggcggtt
     4861 gttccccgta tcgctgaact cgaagccgta agcgcgcagc agggtgaggc tgccgtcggc
     4921 atcgcgcacc ggtttcaggc tggagatcgc cacggtgtcg tcgagaaact gcatgccttc
     4981 ggcaatgcaa atctcgcgcg cggcgcgcac cgccgcttcg cgcgccttca ggctgtccaa
     5041 ccagaaccat gcgatggccg caagcgccag tatgccgaga acttccatga acgcgaagag
     5101 gtaatggcga cgggacaagc ttaacagaac gcgtcccgtc cccggctcgc gccggcgtcg
     5161 tcatttttca ggcggtatgt tgatccgcgg cgcccaggcg atccattcct tcctgggcgc
     5221 gtcgtgcagc acgaacaccg tgccttcctt tgcccgcgcg ctcttgccgt ccggcgtggc
     5281 gaacgcgatg cccccggccg ccagcgatct catcgattcc agcttcagct cgacgccgcg
     5341 gaacaatccc ccgctcacgc tgacgccgct gacgttccag aacaccgagc cgccgcgcac
     5401 caggtccgcg taacgctgcc ggatgaggac gtggatgtcc accgtggccg cattggcact
     5461 gaggtcggcc gattgcacca cgccgacttc gacgccgcgg taatagaccg gcgagttgct
     5521 gcgcagggac ccgacgcggg gagcgcgcag gacgatcttg agccctttca cctcgagcgc
     5581 cgtcggcgcg cgctccaggc cttcgaaatc cgctgcgggc gcgccggtcc ccggcagcgc
     5641 ctggatttcg ggcccggtga gcaccgtgga cagcccggtg atgtcgccga tgcccacttc
     5701 cggccggacg atccagaaca cggagccctg ctgcgcgatg gaggcggccg agcgctggag
     5761 gcgggccttg acggccaccc actgccggtc ttcgctcagc tcgaccgccg tcacctggcc
     5821 gaccggaacg ccgcgatact tgaccggtgt ctggccgatc ttgaccccgc cgccgtcctt
     5881 gaacctgatc gtgatttcgg ggcccaggtt gcgcacgcgg tcgaaaacga gataacccgc
     5941 gacaaccgcg gcgatgacgg gta
//
LOCUS       DBNGSG010000105         9306 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_543496, whole genome shotgun sequence.
ACCESSION   DBNGSG010000105 DBNGSG010000000
VERSION     DBNGSG010000105.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9306)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9306)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9306
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_543496"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="AC158Q_02645"
     CDS             <1..470
                     /locus_tag="AC158Q_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HIR3338694.1"
                     /translation="LGWVEQSALATAMRQELWLYPAVEILHIWGFVVLVGSIAMFDLR
                     LLGLSRGISVRLLSRHLLPWTLGALLVIVPTGLMMFMAHAGEFISNRAFVLKLTLIFA
                     AGINAAAFHLGSFRTVQQWDSGASAPAAAKLHATLSLLIWMGVIACGRLLAYI"
     gene            443..961
                     /locus_tag="AC158Q_02650"
     CDS             443..961
                     /locus_tag="AC158Q_02650"
                     /inference="COORDINATES: protein motif:HMM:NF014639.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HIR3338695.1"
                     /translation="MRTLAGIHLTPRLGYAAGFLVCAGLIGFALYLQYYEYQDPCPLC
                     LLQRTVYMSLMAVFLAAALHGPRRTGAFVYGGLLTATAAIGAAIAARHVWLQHLPPDR
                     VPECGPGLEFTLRKFPLFQALEKVFAGSGQCAEAGWKFLGLTIAGWSLVWFILLGLFC
                     IYLTWPHLRRKT"
     gene            complement(1098..1307)
                     /locus_tag="AC158Q_02655"
     CDS             complement(1098..1307)
                     /locus_tag="AC158Q_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HIR3338696.1"
                     /translation="MIHTADVEGLKLKIYQLHTEHRDLDDVISRLSQSTAQDQLQLQR
                     LKKRKLFLKDQIAMLERQLEPDIPA"
     gene            1484..2728
                     /locus_tag="AC158Q_02660"
     CDS             1484..2728
                     /locus_tag="AC158Q_02660"
                     /inference="COORDINATES: protein motif:HMM:NF012401.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR3338697.1"
                     /translation="MPRREIEGDWVMGWPDRKRATINAGKDSQGRVINGRAPITELEG
                     LMTPTDAYYVVNQLEVPEPVHPDDWVLAITGMVERPMELSLKDLQKLPGRTVRAVTEC
                     AGNDAGFFDYMQRGGKKPSRINQQDMQKRAAMREQNALPSSEEISNESTTTCAMSAGE
                     FTGVPLAEVLKKVGLKPGAAAVRLEGFDRGQPDLAVQYRSVGRADFKLVDPGVINYEK
                     GLPLEKALHADTLLAWSMNGEYLTHIHGAPVRAVTPGWSGNWWVKWLQQIEIMDHVPK
                     CYYQHHYFAYGKSPEDPDREMITSMGVRCIIIDPLDEDSPLERGEHKVRGLAWSGKGG
                     VTRVEVSVDGGKTWRDAHIEEPRERWLWVRWSWLWQVDQPGRYQIMARATDEAGRVQP
                     QIPWNYQRKHFDGIVPTDVTIA"
     gene            2744..3547
                     /locus_tag="AC158Q_02665"
     CDS             2744..3547
                     /locus_tag="AC158Q_02665"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HIR3338698.1"
                     /translation="MHIVVCVKLVPSPEAPFSQFSIDEQARKVVPVAGLQWVMSPFDE
                     QALEAALRVREKHSDARITVVTLGPEFARNALKHGLAMGADDGVLLSDAALQDGDGYA
                     TALALAAAIRKLGGIDLVLTGRQAADWDAGVVGAGIAEMLSTPVISFACDVQVNAAAV
                     RVERVLDDGTEIVEAALPAVVTVSNELGEARKPSLRETMRAARKPVVTWSAAELGVSA
                     HEAGAAGARCVRERVFVPVKDNRCEMVEGATPEAQAAALARRLREAKAI"
     gene            3553..4536
                     /locus_tag="AC158Q_02670"
     CDS             3553..4536
                     /locus_tag="AC158Q_02670"
                     /inference="COORDINATES: protein motif:HMM:NF013200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HIR3338699.1"
                     /translation="MSELLVVVELAQGKPAAVSLELVGLARRLAAQTGGGRVSVLVFS
                     RELAIADLLIARGADRVFTAADPAVAEYNSDISLAYAVKATSAAAATLVLAGHTAQGA
                     DLAPRLAFRLKSVAATGCVSVTADRGVLLFTRSCYGGNARETVSLKTVPAVATVRGGC
                     YDAMPADAARRGDIISLTGEIAVSRIRVIERRRGSGEQARLEDARVIVAGGRGLNGPE
                     GFRLLEELADALGGVVGASRVPCDLGWCSHSMQIGLTGKTVTPDLYIAVGISGASHHL
                     AGCGNAKTIVAVNTDPAAAIFRQAHYGIVGDFQKVVPALATEVRKLGAAQR"
     gene            4707..5171
                     /locus_tag="AC158Q_02675"
     CDS             4707..5171
                     /locus_tag="AC158Q_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014973355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3338700.1"
                     /translation="MAVDIIVNGNTCKVVAEPETPLLYVLRNDLKLKGARFGCGLGLC
                     GACTVLVDGKPVQSCDFPLSAAAGKSITTVEGLAAGGKLHPLQQAFIAEQAAQCGYCA
                     TGIIMAAKALLDANPRPSDADIRTALKGNLCRCGTHQRILKAIRRAAEELAK"
     gene            5168..7288
                     /locus_tag="AC158Q_02680"
     CDS             5168..7288
                     /locus_tag="AC158Q_02680"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3338701.1"
                     /translation="MSESPAPRKLPGNLNTNRRLDRWLRINRDGTVTVFPGKVEIGQG
                     ILTALAQIVAEELDVALERIRLAPTDTTYSPDEGMTSGSQSITDGGVALRYASAEARD
                     LLLQRAAARLGVSLEQLTVADGVVQARSGGAVTYWDLATDDLLAREAPAEIAPKPASS
                     HTIIGTPAPRRDIPAKVSGAPSFVHDLDFPGMLHGRIARPPSYQARLVSLDEAEVRAL
                     PGVVALVRDGSFVGIVAEREEQAIRALTRVKRLARWEEGATLPEPDPRFLLKEATEDE
                     VISEKLDASAAARAAKSVEAEYTKPYIAHAALGPSCAVARCDPDRYTVWTHSQGIYPL
                     RTHLARALGVEERMVTAIHVDGAGCYGHNGADDVALDAALLARALPGRPVRVQWMRED
                     EFQWEPYGPAMVVRLKAGLDATGNIVSWAEDIWSNPHSSRPSGGGVNLIAAWHLAKPL
                     QPSPPRNPPLPGGGSHRNAIPIYEFPNQRVTNRLVKRMPVRVSALRALGATANVFAIE
                     SFMDELAVAAGADPVEFRLRHLKDPRARAVIETVAAKAGWRSSEKGDGARGRGIGFAR
                     YKNGACYLAVIVEVEMEREIAVRRAWAAIDAGLVINPDGLANQTEGGIIQATSWALKE
                     AVQYDRSRILSSNWEDYPILTFQESPQVEVTIVNRPDLPPLGAGEGQQGPTASAIANA
                     IYNAMGVRLRDMPFTRERVMAALA"
     gene            7598..7984
                     /locus_tag="AC158Q_02685"
     CDS             7598..7984
                     /locus_tag="AC158Q_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338702.1"
                     /translation="MPSPVVGQELDLASGRSCRLTPIRNDLIRNYYLLAFPVEQGQPS
                     SAEVTEMLKLGISHAQNLAEELFGDREAFTILYSGYSARREKGWHVHIVLLGNRWRKA
                     WLYAVLAGKNLAQALGLKRDDAPRTR"
     gene            8008..8601
                     /locus_tag="AC158Q_02690"
     CDS             8008..8601
                     /locus_tag="AC158Q_02690"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR3338703.1"
                     /translation="MSAFGFGANEATFRLLSEAYSEKHRHYHTGEHIQACLKHLDVCA
                     AQAEHPKEIEIALWFHDAIYRPLSRNNERKSADWASSFLSANSAPPEVSARVHRLIMV
                     TEHNVAIHTKDESILVDIDLSILGADAKTYDEFEKAVRQEYRMVPGFIYRRKRAEVLR
                     SFLLRPHIYRNEPFLSEREHQARANLSNAVSQLSERA"
     gene            complement(8737..8967)
                     /locus_tag="AC158Q_02695"
     CDS             complement(8737..8967)
                     /locus_tag="AC158Q_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338704.1"
                     /translation="MLIYLDDYRKAKAAKRVDYNSRYDEELLCVNWNPAVSLVALSCY
                     QTPQELSPALPEDSAMVEPAFMDRVYALASQI"
BASE COUNT         1660 a         2899 c         3123 g         1624 t
ORIGIN      
        1 ctctggggtg ggtcgagcaa tcggcgctgg ctacggccat gcgccaagaa ctgtggctct
       61 atcccgcggt cgagatcctg cacatctggg gtttcgtcgt gctggtcggc tcgatcgcga
      121 tgttcgacct gcgcctgctc ggcctctcgc ggggaatttc cgtgcggctg ctgtcgcggc
      181 acctcctgcc gtggaccctg ggggcgctgc tggtcatcgt gccgaccggt ctcatgatgt
      241 tcatggccca tgccggcgag ttcatctcga accgcgcgtt cgtgctgaag ctcacgctga
      301 ttttcgccgc cggcatcaat gccgccgcct ttcacctcgg ctcgttccgc acggtccagc
      361 aatgggacag cggcgcgagc gcgccggcgg cggcgaagct gcacgcgacc ttgtcgctgc
      421 tgatatggat gggggtaatc gcatgcggac gcttgctggc atacatttga ccccgcgtct
      481 cggctacgcg gcgggctttc tcgtctgcgc gggactaatt ggcttcgcgc tctacctgca
      541 gtactacgag tatcaggacc cgtgcccact gtgcctcctg cagcggacgg tgtacatgtc
      601 gctgatggcc gtgttcctag ccgcggcgct gcacgggccg cgccgcaccg gcgctttcgt
      661 ttacggcgga ttgctgaccg cgacggcggc catcggcgcc gcgatcgcgg cccgccacgt
      721 ctggctgcag catctgccgc cggaccgggt gccggagtgc ggccccgggc tggaattcac
      781 gctgcggaag tttcccctgt tccaggcgct cgaaaaggtc tttgccggca gcggacaatg
      841 cgcggaagcc ggctggaaat tcctggggct caccatcgcc ggctggtcgc tggtatggtt
      901 catcctgctg gggctgtttt gcatttatct cacctggccg cacctgaggc gcaagacctg
      961 atcatcggcg acggcaatgc gctgagcttg tcgggcagcg cgtgaaccca caagcgcaga
     1021 atcacgaagt agaaggagca acgtccaggc gttgcatcct gaagtttgat gctcaaaaac
     1081 ctggaatttt cccgccgtca ggcagggatg tcaggttcga gctggcgctc gagcatggcg
     1141 atctggtctt tcagaaagag ttttcgtttc ttgaggcgct gcaactgcag ttgatcctgg
     1201 gcggtacttt gtgacaggcg ggagatgacg tcatcaaggt cgcggtgctc ggtatgcagc
     1261 tgatagatct tcagcttgag accttcgacg tcggcggtat gtatcatggg aactatcctc
     1321 cataccatcg agacggacat ttccagtata gacccaatat tttcaaaaaa cgagcccgct
     1381 tcccgagacg gatgagccgg catggaacga gtcccggtga gggatgtttg aagaagtgct
     1441 agatctttga taatcaccaa tttttttcgt aaaggaatca ggtatgccga gacgcgagat
     1501 cgaaggtgac tgggtgatgg gctggccgga ccgcaagcgc gccacaatca acgccggcaa
     1561 ggactcgcag ggccgcgtca tcaacgggcg ggcgcccatc accgaactgg agggcctcat
     1621 gactcccacc gacgcctact acgtagtcaa tcagctcgaa gtgcccgagc ccgtccaccc
     1681 ggacgattgg gtgctcgcca tcaccggcat ggtcgagcgc ccgatggagt tgtcgcttaa
     1741 ggatctgcag aaactgcccg gccgcaccgt gcgcgctgtc accgagtgcg cgggcaatga
     1801 cgccggtttt ttcgactaca tgcaacgagg cggcaagaaa ccgtcgcgca tcaaccagca
     1861 ggacatgcag aagcgtgccg cgatgcgcga gcaaaacgcg ctgccctcga gcgaggagat
     1921 cagcaacgaa tcgacgacca cgtgcgcgat gagcgctggc gaattcaccg gcgtgccgct
     1981 cgcggaggtc ctgaagaaag tggggctcaa gcccggcgcc gctgccgtac ggctggaagg
     2041 attcgaccgg ggccagccgg acctcgcggt gcagtaccgc tcggtcggcc gcgcggattt
     2101 caagctggtc gacccgggcg tcatcaacta cgaaaaaggc ctgccgctcg aaaaagcgct
     2161 gcacgccgac acactgctcg cctggtcgat gaacggcgag tacctgacgc atatccacgg
     2221 cgcaccggtg cgcgcggtga cgccgggctg gtcgggcaac tggtgggtga agtggctgca
     2281 gcagatcgag atcatggacc acgtgccgaa gtgctactac cagcatcact acttcgcgta
     2341 cggaaagtct cccgaggacc cggacaggga aatgatcact tcaatgggcg tgcgctgcat
     2401 catcatcgac cccctcgacg aggactcgcc tttggaacgc ggcgagcaca aggtgcgcgg
     2461 gcttgcgtgg agcggcaagg gcggcgtgac gcgcgtggaa gtgagcgtgg acggcggcaa
     2521 gacctggcgc gacgcgcaca tcgaggaacc gcgcgagcgc tggctgtggg tgcgctggtc
     2581 ctggttgtgg caagtcgacc agccggggcg ctaccagatc atggcgcgcg ccaccgacga
     2641 ggccggccgc gtgcagccgc agattccatg gaactaccag cgcaagcatt tcgacggcat
     2701 cgtgcccacc gacgtcacca ttgcctgagg gcgcgcgacg cccatgcata tcgtcgtctg
     2761 cgtgaagctg gtgcccagcc ccgaggcccc gttcagccag ttcagcatcg acgaacaggc
     2821 gcggaaagtg gtgcccgtgg cggggctgca atgggtcatg agcccgttcg atgagcaggc
     2881 gctggaggcg gctctgcgcg tccgggagaa gcattcggac gcgcgtatca ccgtggtgac
     2941 gctggggccg gaattcgcgc gcaatgcgct gaagcacggt cttgcgatgg gtgcggacga
     3001 tggtgtgctg ttgtcggacg cggcgctgca ggacggcgac ggctacgcga cggcactggc
     3061 gctcgccgct gcgattcgca aactgggggg catcgacctc gtattgaccg gccgccaggc
     3121 ggccgactgg gatgccggcg tggtcggcgc agggatcgcg gaaatgctga gcaccccggt
     3181 gatttctttc gcctgcgatg tgcaggtcaa cgcggccgcg gtgcgcgtcg agcgcgtgct
     3241 cgacgacggc acggaaatcg tcgaggccgc gctgcccgcg gtggtgacgg tgtcgaatga
     3301 gctgggcgag gcgcgcaagc ccagtctgcg cgaaaccatg cgtgcggcgc gcaagccggt
     3361 cgtgacgtgg tccgccgccg agctgggcgt gtcggcgcac gaggccggcg cggcgggcgc
     3421 ccgctgcgtg cgcgagcgcg tgttcgtgcc ggtcaaggac aaccgttgcg agatggtcga
     3481 aggtgcgacg cccgaagcgc aggctgccgc gctggcgcgg cggttgcgcg aagcgaaagc
     3541 gatataaacg acatgagcga attgctggtc gttgtcgagc tggcgcaggg caagcccgcc
     3601 gccgtgagcc tggagttggt gggcctcgcg cgccgcctcg ccgctcagac agggggcggc
     3661 agggtgtccg tgctggtatt cagccgcgag ctggcaatcg ccgatttgct catcgcgcgc
     3721 ggcgccgacc gggtattcac cgcagccgat cctgctgtgg ccgaatacaa cagcgacatc
     3781 tcgctcgcgt atgccgtcaa ggcgactagc gcggcggccg ccacgctggt gctcgcgggc
     3841 cacacggccc agggtgcgga tcttgcgcca cgcctggcgt ttcgcttgaa gagcgtggcc
     3901 gcaaccggct gcgtctcggt cacggccgac cgcggcgtgc tgctgttcac ccgctcctgc
     3961 tacggcggca atgcgcgcga gaccgtgagc ctcaaaacgg tgcccgcggt ggcgacggtg
     4021 cgcggcggct gttacgacgc gatgccggcg gatgcggcgc gccgtgggga catcatttcg
     4081 ctcacaggcg aaattgctgt ttcgcgaatt cgcgtgatcg agcgccgcag gggcagcggc
     4141 gaacaagccc ggctggagga tgcccgcgtg atcgtggccg gcgggcgcgg attgaacggg
     4201 cccgaaggat tccggctgct cgaggaactg gccgacgcgc tgggcggcgt cgtgggtgcg
     4261 agccgggtgc cgtgcgacct gggctggtgc tctcactcga tgcaaatcgg tttgacgggt
     4321 aagaccgtga caccggacct ctacatcgcg gtgggcattt cgggcgcaag ccaccacctg
     4381 gcgggttgcg gcaacgcaaa gaccatcgtc gccgtcaaca ccgatccggc ggcggcgatt
     4441 ttcaggcagg cgcattatgg catcgtcggc gatttccaga aggtggtgcc ggcgctggcg
     4501 accgaggtga gaaagctggg ggcggcgcaa cgctgatgtc gcgcgaagcc gcatcgttca
     4561 tgattatcct ggcgatttcc ctttcaggtc gtgataatat cgccgcgcct cgagtggatg
     4621 acggaatgct tgccgtacgc ggcggcacac gtccgcgagt gatgaatgcg tagcgattcc
     4681 tgcgaaagag agtaggcctg caaagcatgg cagtagacat tatcgtcaac gggaacacgt
     4741 gcaaggtcgt cgccgagccg gaaacgccgc tgctctacgt gctgcgcaac gatctcaagc
     4801 tgaagggcgc gcgcttcggc tgcgggctcg gcctgtgcgg cgcctgcacc gtgctggtgg
     4861 acggtaaacc ggtccagtcg tgcgatttcc cgctgtccgc cgccgcgggc aaatccatca
     4921 cgacggtcga gggtctggcc gccggcggca agctgcatcc cctgcagcag gcgttcatcg
     4981 ccgagcaggc ggcgcagtgc ggttactgcg cgaccggcat catcatggcc gccaaggcgc
     5041 tgctcgacgc caatccgcgg ccgtcggacg cggacatccg gaccgcgctg aagggcaacc
     5101 tgtgccgctg cggcacgcac cagcgcatcc tgaaggcgat caggcgcgcc gccgaggagc
     5161 tcgcgaaatg agtgaatcgc ccgcgccgcg caagctgccc ggcaatctca acaccaatcg
     5221 ccggctcgac cgctggctga gaatcaaccg cgacggcacg gtcacggtgt tcccgggcaa
     5281 ggtggaaatc ggccagggca tcctcactgc gctcgcccag atcgtcgccg aggagctcga
     5341 cgtggcgctc gagcgcatcc ggctcgcccc caccgatacc acgtacagcc cggacgaagg
     5401 aatgacttcc ggctcccagt cgatcaccga cggcggcgtc gcgctgcgct acgcctcggc
     5461 cgaggcgcgc gacctgctgc tacagcgcgc cgcggcacgt ctcggcgtgt cgctcgagca
     5521 gctcaccgtg gccgacggcg tcgtccaggc ccgctcgggc ggagccgtga cctactggga
     5581 ccttgccacc gacgacctgc tcgcgcgcga ggcacccgcg gagatagcgc ccaagcccgc
     5641 atcttcgcac accattatcg gcacgcctgc gccccggcgc gacattccgg ccaaggtgag
     5701 cggcgcgccc agcttcgtgc acgatctcga ttttccgggc atgctgcatg gacgcatcgc
     5761 gcggccgccg agctaccagg cgcgtctcgt gtcgctggac gaagcagagg tgcgcgcgct
     5821 gccgggcgtg gtggccctgg tgcgcgacgg cagttttgtc ggcatcgtcg ccgagcgcga
     5881 ggagcaggcg atccgcgcgc tcacccgggt gaagcggctc gcgcgctggg aagagggcgc
     5941 cacgcttccg gaacccgacc cgcgcttcct gttgaaggaa gcgacggaag acgaggtgat
     6001 cagcgagaag ctcgatgcct cggccgctgc ccgtgcggcg aagagcgtgg aagcggagta
     6061 cacgaagccg tacatcgcgc atgccgcttt ggggccctcc tgcgcggtgg cgcgttgcga
     6121 cccggaccgt tataccgtgt ggactcacag ccagggcatt tatccgctgc gcactcacct
     6181 cgcccgggcg ctgggggtcg aggagcggat ggtcaccgcg atccacgtcg acggtgccgg
     6241 ctgttacggc cacaacggcg cggacgatgt cgcgctcgac gccgccctgc tggcgcgcgc
     6301 actgccgggc cggccggtgc gggtgcagtg gatgcgcgag gacgagttcc agtgggagcc
     6361 ctacggcccc gcgatggtgg tcaggctcaa ggccggactc gacgcgacgg gcaacatcgt
     6421 cagctgggcc gaggatatct ggagcaaccc gcacagctcg cgccccagcg gcggcggagt
     6481 caacctgatc gcggcatggc acctcgccaa gccgctgcag ccgtcgccgc cgcgcaaccc
     6541 gccgctgccg ggcggcggca gccatcgcaa cgccatcccg atctacgagt ttcccaacca
     6601 gcgggtgacc aaccggctcg tcaagcgcat gccggtgcgg gtgtccgcgc tgcgcgcgct
     6661 gggcgcgacg gccaatgtct ttgccatcga gtcgttcatg gacgagctgg ctgtcgcggc
     6721 tggcgccgac ccggtcgaat tccggctgcg ccatctcaag gatccgcgcg cgcgcgcggt
     6781 gatcgagacc gtcgccgcga aggccggctg gcgcagcagc gagaagggcg acggcgcgcg
     6841 cgggcgcggc atcggttttg cgcgctacaa gaacggcgcc tgctacctgg cggtgatcgt
     6901 ggaagtggag atggagcgcg agatcgcggt ccggcgcgcc tgggcggcaa tcgacgccgg
     6961 cctcgtgatc aatcccgacg ggcttgccaa ccagaccgag ggcggcatca tccaggccac
     7021 gagctgggcg ctcaaggaag cggtgcagta cgatcgcagc cgcatccttt cgagcaactg
     7081 ggaggactac ccgatcctca ctttccagga gtcgccgcag gtggaagtga ccatcgtcaa
     7141 ccggcccgac ttgccgccgc tcggcgcggg cgagggacag caggggccga ccgcctccgc
     7201 gattgcgaac gcgatctaca acgccatggg cgtgaggctg cgcgacatgc ccttcacgcg
     7261 cgagcgcgtt atggccgctc tggcctgaga gataagggcg tgaatcgcga ttcgtgagtc
     7321 gtgactcgac tcccctgctg tctgaggagg agtggcgcga agcgccaggg tgttgggggc
     7381 aagacacttt cgactttcgc cttccgactt tcgacttaag tattacgatc ccttcgcggg
     7441 ggctgtgtga cttcctttgc tcccgatgga cgctggttgc cgactggcgt aacgtatttg
     7501 gccagataga cgtgtggtga cggggcttgg cgtgacagcg gcggacgtca actagcaagt
     7561 caacccaatt accacgcgct ttaaagacgg gtccgaaatg cccagcccag tcgtaggtca
     7621 ggaacttgat ttggcatcgg gcaggtcctg ccgtctgacg ccgattcgca acgatctgat
     7681 ccgtaactac tacctgctcg cattccccgt ggaacagggc cagccttcgt ctgccgaggt
     7741 cactgaaatg ctgaaactgg gcattagcca tgcgcagaat cttgctgaag aactctttgg
     7801 cgatcgcgag gcgttcacga tcttatacag cggctacagc gctcgcaggg agaaagggtg
     7861 gcacgtacat atcgtccttt tgggtaaccg ctggagaaaa gcttggcttt acgcggtctt
     7921 ggccggcaaa aacctcgctc aagcgctggg gctcaaaagg gacgatgccc cacggacccg
     7981 atgaacaggg aaagatggtc aaggcttatg agcgcgtttg gcttcggggc caatgaagcc
     8041 acgtttcgtt tattgagcga agcgtattcg gagaagcaca ggcactatca caccggggaa
     8101 catattcaag cctgcctgaa acaccttgat gtctgtgctg cccaggcgga acacccgaaa
     8161 gaaatcgaga ttgcactttg gtttcacgat gcgatctaca ggcccctttc gcgcaacaac
     8221 gagcgaaaaa gcgccgattg ggcttcaagc ttcttgtcgg ccaactcggc ccctccggaa
     8281 gtgtcagctc gtgttcaccg cttgatcatg gtgaccgagc ataacgtcgc catacatacg
     8341 aaagatgagt cgatcctcgt ggatatagac ctgtctatcc tgggggccga tgcgaagacg
     8401 tatgacgaat tcgaaaaagc cgttcgacag gaatatcgaa tggttcccgg gttcatctac
     8461 cgccggaaga gagcggaagt gctgcgcagc ttcttgctgc gccctcacat ttaccgaaac
     8521 gagccttttc tgtcagagcg ggagcaccag gcgagggcca acctttccaa tgccgtgtcg
     8581 cagttatcag agcgtgcatg acgatgggtt ggcagtggcg ttcggctaaa gtgcgggcct
     8641 tggcatcgga gtcggcgttg tgattccggc gccgacggca cgaaaaaaaa agcggaccgg
     8701 ccacgagggc acggcccgcc tccgggggag acgtttctag atctggctcg cgagggcgta
     8761 gacccggtcc atgaaggcgg gctcgaccat cgcggaatct tccggcagcg ccgggctcag
     8821 ttcctggggg gtctggtagc aggacagcgc aaccaggctc accgcgggat tccagttcac
     8881 gcaaagcaac tcttcgtcgt agcggctgtt gtaatcgacg cgcttggccg ccttcgcctt
     8941 gcggtaatca tccaggtaga tcagcatggc cttctcccga gctgtgttcg tttcctgccc
     9001 ggactgcgtg caggttccat gccaatagaa gggcgcaggt cgcccttata tataattacc
     9061 tgatttgatg ggtattgttt gtttggattg tggggcacgc ccgagctggc ttatgtcgaa
     9121 tgccggacaa tagttccgac gttcgacatt gcgatatcga aatctcgaca gcctccggcc
     9181 accggcgtga aataccgcac aacctataaa actgcgccag cgcggcccga gttgttccgc
     9241 ccggctcgcc caggccccgc cccacaccac ccccccccgg agaagaacct gtccccgccc
     9301 cagtcc
//
LOCUS       DBNGSG010000106         8927 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3940250, whole genome shotgun sequence.
ACCESSION   DBNGSG010000106 DBNGSG010000000
VERSION     DBNGSG010000106.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 8927)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8927)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8927
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3940250"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..1402
                     /gene="purB"
                     /locus_tag="AC158Q_02700"
     CDS             38..1402
                     /gene="purB"
                     /locus_tag="AC158Q_02700"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HIR3338705.1"
                     /translation="MASGVFDDALIRHLWSTDELRAIFSDASRVQKWYDFEAALALEQ
                     AELGIIPKAAAQDIAAQAKVANVDLSSIGAEIRRIKHPLVPALKAVEKLCRDGHGEYI
                     HFGPTTQDVLDTATVLQMKEAHAIYLRDMKAIGRALCKLAEAHKATPMAGRSHGVQAL
                     PITFGHKAAVWLAEMGRNYERLRGLEARTFVGGMVGAVGTQASYGPQAPELERRLMKR
                     LGLGVADINWQPARDRFAEYACVLAIIGQTLGKIANEIINLEHTEVGELYEPFSEGKV
                     GSSTMPHKRNPSSCEAVVGVSRALRYNAALLLEGMIIEHERDGSSWRAEWKALPESCL
                     MAGGVLATMKYVLGGLTVDAPRMRRNLDLLGGFLLSERVMFALSEKVGKQTAHDLVYE
                     ASMHGIEHGIGFEQALMQDPRVAAALPPAELRRVLDPATYVGLAPQIVDSVLAQTRAS
                     GWLD"
     gene            1580..2563
                     /locus_tag="AC158Q_02705"
     CDS             1580..2563
                     /locus_tag="AC158Q_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HIR3338706.1"
                     /translation="MKIAILDDYQNVVRTLRCLSRLAGHDVTIWNDHTKDADVLAQRL
                     KDTEALVTIRERTPIRAPLIERLPKLKLVSQTSVYPHLDVDALTRRGILLCSDLHPGE
                     PSFATAELAWCLIISALRSVPQEVAALKSGRWQSTIGHCLRGRTLGVLGYGRIGAVLA
                     RYARAFEMDVLAWGRARSAERARADGVAFAASREELLERSDVLTLHVRLTPETRGLVT
                     AADLARMKPDSLLVNTSRAGLIEPGALVAALKAGRPGMAAVDVYEEEPMLNPDHPLLK
                     MPNVVCTPHLGYVEREGYERIFSAAFDQILAFVAGKPFNVINPEALQNRDS"
     gene            2739..4940
                     /locus_tag="AC158Q_02710"
     CDS             2739..4940
                     /locus_tag="AC158Q_02710"
                     /inference="COORDINATES: protein motif:HMM:NF024491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HIR3338707.1"
                     /translation="MDLPAQAQDAPRTDAVPGSLFEAGRNCLKAVRAHRAALLVDGED
                     YFAAFMRAAERAQRSLLIVGWDFNSRTRLRWDGNPGGAPAVLGDFLNYLARRRRRLRI
                     HILDWDFPMVFGTDREFPLLYGTTWKPHRRVQLCYDNTHPTIGSHHQKIVVIDDAMAF
                     SGGLDLTCRRWDSRRHNAVDPRRVTDGQPYPPFHDAMMAVDGEAARVLAAIARERWLG
                     ATGKTLTPVSVTEDPWPPGLAADLKDVDVAVSRTVPLTATPEVREVEALYLDMVARAR
                     RYIYIENQYFTSHRIGAALAARLEEPGGPEVVVVSRLLSHGWLEEHTMHVLRTRLVKL
                     LRQSDRGGRFHIYYPHVPGLPDGTCVDVHSKIMVVDDEWLRVGSANLCNRSMGMDTEC
                     DLTVEARGRPETTRAVREFRNGLLGEHLGLDSARVQQEIERSGTLSGAIEALRNDGRS
                     LRPLAELPDWPDTVIGLAEITDPERPVSMDQLILEFSPDSAEQKPARFAWGRALAVAA
                     VIAGLMAMWRYTPLAGLVSAERIIEWAEDFAGRPWAPFALVAAYTPASFVMFPRPLIT
                     LAAVVAFGPWLGAAYAITGILLAAIANYLVGRMMNRARVRRIAGERLNRISEVLRRRG
                     LVAMTAVRLVPIAPFPVVGLVAGAIRVRFSHYVLGTLFGMLPGAIVTTVFGDQLQSAL
                     RDPSRINYWIIAGVVIVFAAGIYAVRRWFHAQFEKQGEQPHTQGVPQPGAA"
     gene            5314..6273
                     /locus_tag="AC158Q_02715"
     CDS             5314..6273
                     /locus_tag="AC158Q_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3338708.1"
                     /translation="MAIAMFAQASFVPGATVAQQYPTKPIRLIVPFAPGGPNDILGRL
                     VGQKLTEQLGQTVVVENRGGAGGTIGLDAAAKSPGDGYTLSMGGSSNMTVAPALYTKL
                     PYDSLKDFTPVILVAHVPYAVGVNPTVPAKNVKELIAIAKRKANYLSYGSSGTGSMSS
                     LSAELFKSLSGTRIVHVPYKGTAPALTDVVSGQIDMMFADLSLIQNLARGGRLRLVAV
                     TGSKRLASAPGVPTIAESGIKGYQIEPWFGVVGPAGVPGAIVSRLNGVIAAGLKSADV
                     TQRLDALGYEAIGGTPEQFAATIRADIPRYAKIVKTAGIKAEL"
     gene            7033..7914
                     /locus_tag="AC158Q_02720"
     CDS             7033..7914
                     /locus_tag="AC158Q_02720"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3338709.1"
                     /translation="MIIPFAPGGASDFIGRILQPKLAEVLGQPVVVDNRAGASGNIGV
                     EHAARATPDGYTFLLGNVSTMGIGPSMFPDFPVRALRDFIAITLVVDVPGALGIHGGL
                     PPNNLKEFIAYAKQHDGKLNYGSAGLASAQGMAFAFFMNRAGIRLQQVPYKGGAGAAT
                     IATLSGEVAATMVTTASLVPHAKSGRIKILAVVAKKRVPALPSTPTMIESGFPELDLG
                     SWQGIFVPLGTPRPIVDQLYKVVTRVVHDPWTGEQYAKASAVQLTSDSPADFAKFMKV
                     QNAFWGKIIKDLGIKTQ"
     gene            complement(8060..8554)
                     /locus_tag="AC158Q_02725"
     CDS             complement(8060..8554)
                     /locus_tag="AC158Q_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020564292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3338710.1"
                     /translation="MKNQAKNTICLWYNGSAEEAAHFYAKTFPDSSVDALHRAPGDFP
                     SGKEGDVLMVEFTVMGIPCLGLNGGPAFKHNEAFSFLVATSDQAETDRYWHAIVGNGG
                     EESECGWCRDKWGISWQITPVALTEAVTDPDRAAAKRAFNAMMEMRKIDIAAIEAARR
                     GGSR"
     gene            complement(8652..>8927)
                     /locus_tag="AC158Q_02730"
     CDS             complement(8652..>8927)
                     /locus_tag="AC158Q_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338711.1"
                     /translation="ARTGDVGRNDAAVEHFAAASKASASCDLVRLALTWRDAGPSSSD
                     KSAIAAIAAGVLPLYHEFLSDYEPRLREFARPDLAESFYKWRQRLSA"
BASE COUNT         1594 a         2856 c         2934 g         1543 t
ORIGIN      
        1 cgaatcacaa ttcatggttc acgcaattgg ggaaacaatg gcttccggcg tattcgacga
       61 cgcattgatc aggcacctgt ggagcaccga cgagctgcgc gcgatattca gcgacgcgag
      121 ccgggtgcag aagtggtatg acttcgaggc ggcgctggcg ctggagcagg cggagctcgg
      181 catcatcccg aaggcggcgg cgcaggacat cgcggcgcag gcgaaggtcg cgaacgtcga
      241 tctctcatcg atcggcgccg agatccgccg catcaagcac ccgctggtgc cggcgctgaa
      301 ggcggtcgag aagctgtgcc gggacggcca cggtgaatac atccatttcg ggccgaccac
      361 ccaagacgtg ctcgacaccg ccaccgtgct gcagatgaag gaagctcacg cgatctatct
      421 gcgcgacatg aaggccatcg gccgggcgct ctgcaagctg gccgaagcgc acaaggcgac
      481 gccgatggcg ggccgctccc acggcgtgca ggcgctgccg atcacgttcg gccacaaggc
      541 ggcggtctgg ctcgccgaaa tggggcgcaa ttacgagcgc ctgcggggcc tggaggcgcg
      601 cactttcgtc ggaggcatgg tgggcgcggt cggaacgcag gcgtcctacg gcccgcaggc
      661 gcccgagctc gagcgccggc tgatgaagcg cctggggctc ggcgtggcgg acatcaactg
      721 gcagccggcg cgcgaccgct tcgcggaata cgcctgcgtg ctggcgatca tcggccagac
      781 gctcggcaag atcgcgaacg agatcatcaa cctagagcac accgaagtag gcgagctcta
      841 cgagccgttc agcgagggca aggtcggttc ctccaccatg ccgcacaagc gcaacccgtc
      901 ttcctgcgag gcggtggtcg gcgtctcccg cgccctgcgc tacaacgcgg cgctcctgct
      961 cgagggcatg atcatcgagc acgagcgcga cggttcgtcc tggcgcgccg agtggaaggc
     1021 gctgcccgag tcctgcctga tggcgggcgg ggtgctcgcc acgatgaagt acgtgctcgg
     1081 cgggctgacg gtggacgcgc cgcgcatgcg caggaacctc gacctgctgg gtggcttcct
     1141 gctctccgag cgcgtcatgt tcgcgctgtc ggaaaaagtc ggcaagcaga ccgcgcatga
     1201 cctggtgtac gaggcttcga tgcacggcat cgaacacggc atcggtttcg agcaggcgct
     1261 gatgcaggac ccgcgcgtgg ccgcggcatt gccgccggcg gagctgcgca gggtgctcga
     1321 ccccgcgacc tacgtcggcc tcgcgccgca gatcgtggac agcgtgcttg cgcaaacgcg
     1381 cgccagcggc tggctcgatt aaaccagtgt tgaattttga gttttgagtg ttgagttgca
     1441 aacaacctgg aaccgggatc actcctaaga aacctctgca acggccgttt ttcgtcattc
     1501 ccgcgcaagc gggaatccag ccggagcggc gtgtctcaag gccgaaactc aacactcaac
     1561 attcaaaact caaaactcga tgaaaatcgc catcctcgac gactaccaga acgtcgttcg
     1621 cacgctgcgg tgcttgtcca ggctcgcggg gcacgacgtc accatctgga acgaccacac
     1681 caaggacgcg gacgtgctgg cgcagcggct gaaggacacc gaggcgctgg taacgatccg
     1741 cgagcgcacg ccgatccgcg cgccattgat cgagcggctg ccgaagctta agctcgtcag
     1801 ccagaccagc gtctacccgc atctcgacgt ggatgcgctg acgcgccgcg gcatcctgct
     1861 gtgctccgac ctgcacccgg gcgagccgtc gttcgccacc gccgagctgg catggtgcct
     1921 gatcatctcc gcgctgcgca gcgtgccgca ggaagtggcg gcgctgaaga gcgggcgctg
     1981 gcagtccacc atcggccact gtctgcgcgg gcgcaccctc ggcgtcctgg gttacgggcg
     2041 catcggagcg gtgctggcgc gttacgcgcg ggccttcgag atggacgtgc tggcgtgggg
     2101 acgagccagg tcggcggagc gggcgcgggc cgacggcgtc gcgttcgccg cttccaggga
     2161 ggagttgctc gagcgctcgg acgtgctgac actgcacgtg cgcctgactc ccgagacgcg
     2221 cggcctcgtg acggcggcgg acctcgcgcg catgaagccc gattcgcttc tcgtcaacac
     2281 cagccgcgcc ggcctgatcg agccgggtgc cctcgtcgcc gccctgaagg cgggacgccc
     2341 cggcatggcc gcagtggacg tgtacgagga ggaacccatg ctgaatccgg accacccgct
     2401 gctcaagatg cccaacgtgg tgtgcacgcc gcacctgggc tatgtcgagc gcgagggcta
     2461 cgagcgcatt ttcagcgcgg cattcgacca gatactggcg ttcgtcgccg gcaaaccgtt
     2521 caacgtgatc aacccggaag cgctgcaaaa ccgtgattcg tgagtcgtga ttcgaccgac
     2581 gggcgttagc agaagagcga ggtttccgca cgcgatttcc gtgaacgata gcggatcggg
     2641 atggcctaat ttcctgagac gctccattct gcccgtcgcc aagcggcgac agggttttat
     2701 tttccccggc gaaacggcgc atcccaagga attcgaacat ggatcttcct gcccaggcgc
     2761 aagatgcgcc gcgaacggac gcggttcccg gcagcctctt cgaggcgggc cggaattgcc
     2821 tcaaggccgt tcgcgcgcac cgcgccgcgc tgctggtcga cggcgaggat tatttcgcgg
     2881 cattcatgcg ggccgccgag cgcgcgcagc ggtcgctact gatcgtcggc tgggacttca
     2941 acagccgcac ccgcctgcgc tgggacggga atcccggcgg cgcgccggcc gtcctcgggg
     3001 acttcctgaa ttaccttgcg cgccgccgcc gccggctgcg catccacatc ctcgactggg
     3061 atttcccgat ggtgttcggc accgaccggg aattcccgct gctctacggg acgacctgga
     3121 aaccgcaccg gcgcgttcag ctctgctacg acaatacgca cccgaccatc gggtcgcatc
     3181 atcagaagat cgtggtgatc gacgatgcca tggcgttttc gggcggcctg gacctgacct
     3241 gcaggcgctg ggactcccgc cgccacaatg ccgttgaccc acggcgcgtc accgacgggc
     3301 aaccgtaccc gccgttccat gacgccatga tggccgtgga cggagaggcc gcgcgcgtgc
     3361 ttgccgcgat tgcgcgcgag cgctggctcg gcgcgacggg caagacgctc actccggtgt
     3421 ccgtcacgga ggacccgtgg ccgcccgggc tcgcggcgga cctgaaggac gtcgacgtag
     3481 ccgtatcgcg cacggtgccg ttgacggcga cgccggaagt gcgcgaggtg gaggcgctgt
     3541 atctggacat ggtcgcgcgg gccaggcgct acatctacat cgaaaaccag tacttcacct
     3601 cgcaccggat cggggcggcg cttgccgccc ggctggagga gcccggcggg ccggaagtcg
     3661 tggtggtgtc gcgcctgctg agccatggct ggctcgagga gcacacgatg cacgtgctgc
     3721 gcacgcggct ggtcaagctg ttgcgccagt cggacagggg cgggcgtttc catatctatt
     3781 acccgcacgt gccgggcctg cccgacggca cctgcgtgga cgttcactcg aagatcatgg
     3841 tggtggatga cgagtggctg cgggtgggct cggcgaatct ctgcaaccgc tcgatgggca
     3901 tggacacgga atgcgacctg acggtggagg ctcgcggcag gcccgaaacc acgcgcgcgg
     3961 tgcgcgaatt ccgcaatggc ctgctgggcg agcacctcgg tctggattcc gcgcgggtgc
     4021 agcaggaaat cgagcgcagc ggcacgctca gcggcgcaat cgaggcgctg cgcaacgacg
     4081 ggcgctcgct gcgcccgctc gcggaattgc cggattggcc cgataccgtg atcggcctcg
     4141 ccgagatcac cgacccggag cggccggtct cgatggacca gctcatcctg gaattctccc
     4201 ccgacagcgc cgagcagaag cccgcgcgtt tcgcgtgggg cagggcgctt gccgtcgcgg
     4261 cggtgattgc cgggctcatg gccatgtggc gctatacgcc gctcgccggg ctggtgagcg
     4321 ccgagcgcat catagagtgg gccgaggatt ttgccggcag gccgtgggcg ccatttgcgc
     4381 tcgtcgcggc ctacaccccc gccagcttcg tcatgtttcc aaggcccctc atcacgctcg
     4441 ccgcggtggt tgctttcggc ccgtggctgg gcgccgccta tgccataacc ggaatactcc
     4501 tcgcggcgat cgccaactat ctggttggcc gcatgatgaa ccgcgcccgg gttcgcagga
     4561 tcgcgggcga gcggttgaat cgcatcagcg aagtcctgcg caggcggggg ctggtcgcca
     4621 tgaccgcggt gcggctcgtc ccgattgcgc cgttcccggt cgtcgggctg gtcgcgggcg
     4681 cgatccgggt caggttcagc cactacgtgc tgggcaccct gttcggcatg ctgcccggcg
     4741 cgatcgtgac cacggtcttc ggcgaccagc tgcaatcggc gctgcgggac ccttcccgga
     4801 tcaactattg gatcattgca ggcgtcgtga tcgtgttcgc ggccggcatc tacgccgtgc
     4861 gccgatggtt ccacgcgcag ttcgagaaac agggcgagca accgcatacc cagggcgttc
     4921 cgcagcccgg cgccgcctga gtttttttct ggtccggcac ggaccttgct cgatatggaa
     4981 ggcggggcgg caagttcccg tgtcctcgcg tcagtggcca tccgctgtgt tcgtggaaac
     5041 ggcttgcgaa tgcgagagtt ataatttgcc ggcctcgggg agattctgat gaccaataag
     5101 acgttgtttg cgcgcgcggc agcttatgcg tgcctggccg tgacggcata cacccatggg
     5161 gtgcacgcgc agaccgcggc gtatccggtc aagccgatcc ggctgattgt tcctttcgcg
     5221 caaggcgggg gccccggatc tcggcgaagg ggttggtttt ggggcggaga cggggccttg
     5281 ccgccgggtt ggtggcacgg cgtcctgcgg tggatggcaa tcgcgatgtt cgcgcaggcg
     5341 tcgtttgtcc cgggagcaac ggttgcgcaa cagtacccga cgaagccgat acggctgatc
     5401 gtcccgttcg cgccaggcgg ccccaatgac attctcggcc gcctggtggg gcagaaactc
     5461 acggaacagc tcgggcagac cgtggtggtg gaaaatcgcg gcggcgccgg cggcaccatc
     5521 ggactggatg ccgcggccaa gtccccgggc gacggctaca ccctgtccat gggcggatcc
     5581 agcaacatga cggtggcgcc cgccctctac acgaagctgc cgtacgactc gctgaaggat
     5641 ttcacgccgg tgatcctggt cgcccatgtg ccgtacgcgg tcggcgtgaa tcccaccgtg
     5701 ccggcgaaga acgtgaagga actgatcgcc attgcgaagc ggaaagcgaa ttacctgagc
     5761 tacggttcct ccggcaccgg cagcatgtcg agcctgtccg ccgagttgtt caaatcgctg
     5821 tccggcaccc ggatcgtgca cgtgccctac aagggcacgg ccccggcgct gaccgatgtc
     5881 gtcagcggcc agatcgacat gatgttcgcg gatctgtcgc tgatccagaa cctggccagg
     5941 ggcggcaggc tgcggctggt ggcggtcacc ggttcgaagc gcctcgcctc ggcgccgggc
     6001 gtgcctacca tcgccgagtc gggcatcaag ggctaccaga tcgagccgtg gttcggggtg
     6061 gtcggccccg ccggcgtgcc gggcgcgatc gtttcccggc tgaacggcgt gatcgcggcg
     6121 ggactcaagt ccgccgacgt gacgcagcgc ctggacgcgc tcggttacga ggccatcggc
     6181 ggcacgccgg agcagttcgc cgcgaccatc agggccgaca ttccccgcta cgcgaagatc
     6241 gtgaagacgg cggggatcaa ggcggagctt tgacgaccgg tgatgagtga taagtcatga
     6301 gtgacggaaa caaacctgaa ccgccaagca tgtcctgagc ccgggcctgt cctgagcctg
     6361 tcgaaggaac gccaaggccg ccaggaaaag cgaaacaata actgaaccgc tgggctgtcg
     6421 agagaaatac gaaatagaaa ggcttaagga acctctgcat aagtcatccc cgcgcaggcg
     6481 gggatcccgt aacgatttgt ttaccctgga ttcccgcctt cgcgggaatg acgaaaaacg
     6541 gcttttgcag agcatcctta acaggatcga aaatcgacag aggcaaggga aaatgaccat
     6601 ggccggctgg aaacagccgg ccgtttcatt ttcaggggga aatttatagg catttgaaat
     6661 aattgcattt gtaatggcgt aatgggcccc gaccctgaga tcgtctctta atgagatcgc
     6721 tctacccagg ccagctgggt tattcttgaa ctgcggctgt ttcctgctca ccgggcaggc
     6781 agtcaaggtc gctgtagcca cgggcaaatc atcacccgca gcctggtcgc ctcctgcgcc
     6841 gggctgagcg gctccgggac cgccgccgcg tcgcgaacat aaaaatagtt gaaaccatcg
     6901 acacatcgag gagaccacac catgcaactc aagcacaacg cgttttgcgc ggcgatcgcc
     6961 gccgcttgca cgtttcacgt cagcgcgccc gcgtggggac aggcagaagc ctttccttca
     7021 cggcctctgc ggatgatcat tcctttcgcg ccgggcggcg cgtcggattt catcggccgc
     7081 attctgcagc ccaagctcgc ggaggtgctc gggcagccgg tggtcgtcga caaccgcgct
     7141 ggcgcttcgg gaaacatcgg agtggagcac gctgcgcgcg cgacaccgga tggctacacg
     7201 ttcctgctcg gcaacgtcag caccatggga atcggcccga gcatgttccc ggatttcccg
     7261 gtgcgcgcgc tgcgggattt catcgccatc acgctggtgg ttgacgtgcc gggcgcactg
     7321 ggcatccacg gcggcctgcc accgaacaac ctgaaggagt tcatcgccta cgccaagcag
     7381 cacgacggca agctcaatta cggctctgca gggttggcca gtgcgcaagg catggcgttc
     7441 gcgttcttca tgaacagggc gggaatcagg ctccagcagg taccgtataa aggcggggcc
     7501 ggcgccgcga cgatcgcgac cctctccggc gaagttgccg ccacgatggt caccaccgct
     7561 tccctggtgc cgcacgccaa gagcggaagg atcaagatcc tcgccgtggt tgcgaagaag
     7621 cgcgtgccgg cccttcccag tacgccgacc atgatcgagt cgggattccc ggaactcgac
     7681 ctcgggtcct ggcagggaat cttcgtgccc ctaggcacgc cgcgcccgat cgtcgaccag
     7741 ctctacaagg tcgtcacccg cgtggttcac gacccctgga ccggggaaca atacgcgaaa
     7801 gcgagcgccg tgcagctcac cagcgattcg ccggcggatt tcgcgaagtt catgaaggtg
     7861 cagaacgcgt tctgggggaa gatcatcaag gatctcggca tcaagaccca gtaggcaagc
     7921 ctattggacc gccagggcac gttaaatccg gacgctgcgc accggtttaa ctccgcgcgt
     7981 tcgagcggac gcatctgcgc cgttcaacgc gcggaaaccg gcttgaggat taacgtttga
     8041 actcgggcta ttgcccacac tacctgctgc caccccggcg tgcagcctcg attgccgcaa
     8101 tgtcgatttt cctcatctcc atcattgcat tgaacgcgcg cttggctgca gcgcggtcgg
     8161 gatcagttac cgcctccgtc aaggccaccg gcgtaatttg ccaggagatt ccccatttgt
     8221 ccctgcacca gccgcactcg ctttcttcac cgccgttgcc aacgattgcg tgccagtaac
     8281 gatccgtttc cgcctggtcg gacgttgcaa ccagaaatga gaacgcctcg ttgtgcttga
     8341 aggcgggacc gccattaagc ccgaggcagg gaatacccat gacagtaaac tcaaccatca
     8401 ggacatcccc ttccttcccg gatggaaagt ctcctggcgc gcggtgcagt gcatcaacgg
     8461 atgaatcagg aaaggttttc gcgtaaaagt gcgcagcctc ttcggcactg ccgttgtacc
     8521 aaaggcaaat cgtgttcttc gcttgattct tcatgtcacc tctccatgca aaggcggcta
     8581 acgttccgct cggccgcgca cttcgccgcg tcggctgccg cgcgctgata gccggctagt
     8641 gagaatttca gtcatgccga tagcctctgc cgccacttat agaacgactc cgcgaggtcg
     8701 ggcctcgcga actctcggag tcgtggttca tagtcggaga ggaactcatg atagagcgga
     8761 agaacgcccg cagcgatcgc cgcaattgcg ctcttgtccg aggaggatgg gccagcatcg
     8821 cgccacgtga gcgccaagcg caccaaatcg caggaagcgc tcgccttgct ggcggcggcg
     8881 aaatgctcga cggccgcatc gtttcggccg acatctccgg ttcgcgc
//
LOCUS       DBNGSG010000107        10888 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_715668, whole genome shotgun sequence.
ACCESSION   DBNGSG010000107 DBNGSG010000000
VERSION     DBNGSG010000107.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 10888)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10888)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10888
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_715668"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="AC158Q_02735"
     CDS             <1..317
                     /locus_tag="AC158Q_02735"
                     /inference="COORDINATES: protein motif:HMM:NF024578.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HIR3338712.1"
                     /translation="LASGRSRAPARPWLGVYSEEIQGHVVVTRVLPESPAAQAGLARG
                     DIILGVGGEAVGRQSEFYQRLWSSGDAGSPVILHVLHKRTVRQLTVRSMDRMAFLRPW
                     QA"
     gene            complement(413..1780)
                     /locus_tag="AC158Q_02740"
     CDS             complement(413..1780)
                     /locus_tag="AC158Q_02740"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004065 - arylsulfatase activity [Evidence
                     IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HIR3338713.1"
                     /translation="MAQHRNPQTPNIVLILADNLGWGELGCYGGGVLRGAPTPRIDKL
                     ATEGLRLTNFNVESDCVPTRSALMTGRHPIRTGALQAVPAGLPQGITPWEITLAQLLS
                     GKGYATAIFGKWHLGDRTGRFPIDRGFDEWYGIPRTTNESMFTSSPGYDPQAVPLPYV
                     MEGRKGGPARNVAVYDLEMRRRIDSDLAARACDFMRRQAAAGKPFFAYVPITQLHYPT
                     LPHRDFAGRTGAGDFADSMAEMDHRVGQVLDEMDALKIAGDSLVIFASDNGPEFRRPW
                     RGTAGPWTGTYHTAMEGGLRAPFMIRWPGRVPPGRVSNEIVHVTDLFTTLARVAGAAL
                     PTDRPIDGVDQLDFFLGSSEKSGREGFVYYIKNELRAVKWRDWKMHLVWEAEPNAGPN
                     HLESPLIFNVIQDPKEETDLHVVSSWVRTPMRRLIHEFQEGLKAHPPIPPGAPDDYAP
                     RGGNA"
     gene            1952..2680
                     /locus_tag="AC158Q_02745"
     CDS             1952..2680
                     /locus_tag="AC158Q_02745"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011549455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR3338714.1"
                     /translation="MRVGLFVTCLVDLMRPRIGFSAIRLLEAAGCDVVVPQGQTCCGQ
                     PAYNSGDRATARSLALKVLDEFEGCEYVVVPSGSCAGMIRAHFPGLFTAEPAELGRCR
                     ELAARTYELTDFLANVAKLQQVPGTFHGTVTYHDSCSGLRELGVKSQPRALLAKVPGL
                     ALKEMNEAETCCGFGGAFAVKFGEISAHIAERKCANIAESGADAVVLGDLGCMLNIEG
                     RLRRRGDMKTKVLHVAEVLAGKIE"
     gene            2864..3790
                     /locus_tag="AC158Q_02750"
     CDS             2864..3790
                     /locus_tag="AC158Q_02750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HIR3338715.1"
                     /translation="MKIAVMGSGGVGGFYGGRLAHTGCDVTFVARGAHLKAMRESGLT
                     IENEAQGNIHLSRVKVVEDPAAIGPVDLVIIAVKLWDTESAARAVKPIVGPDTAVLSL
                     QNGVIKDDILRREFGDRAVMGGVAYVGTNIARPGVIRQIGTLQRLIFGEYDGRRSARA
                     EALLAALLRAGIQAELSGDIRRTLWEKYSFLVGLSATTAAMRVPIGAIRSNPQTRAFL
                     FDILKETVAVGRALGVALPENYAEDRLAFADSVPADMTSSLHHDLERGNPLEVEWLSG
                     GVVQLGKQAGVPTPMNRAVWDILALHARGRKV"
     gene            3807..4901
                     /locus_tag="AC158Q_02755"
     CDS             3807..4901
                     /locus_tag="AC158Q_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HIR3338716.1"
                     /translation="MNAPRLTVRNLRATPVLVPMTYVLGTSAQAVREAPLLLVDLETE
                     EGVTGHAYQFCYLPAAAPAIMILLDEVLRTVAGDRAEPVALWEKLTKRFTLIGVQGVV
                     RMAMAMLDVACWDALAKAAGKPLAAFLGGSPGAVPAYNSCGLGLMAPAAVADEAEQLL
                     AGGFRAVKLRLGYPTPEADLAAVRAVRNRIPDDVALMVDYNQALTVQEAIRRGRALDG
                     EGVYWIEEPVRHDDFTGCAEIARALSTPVQIGENFSQVHDMERALAAQACDYVMPDLE
                     RIGGVTGWRRAGELAGARRIRMSSHLYPEVSAHLLAATPAAHWLEYVDWANAVLAEPL
                     RISDGHAVVSGHPGNGLAWNADAVRRFRMN"
     gene            4919..5773
                     /locus_tag="AC158Q_02760"
     CDS             4919..5773
                     /locus_tag="AC158Q_02760"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3338717.1"
                     /translation="MSNTLKGIVVMAVGVALLTANDAISKYLTESHPVGQVICLRQAA
                     TLLVIVPYVMLVTGWGALRVVSWPGQLTRGLLFVANAALIVLGFSLLPLATVITIMFS
                     SPIFLALLSASLLTERVTLDRWIAIIGGFAGVLIVIRPGATAFDWVLLIPVGAALANA
                     LRDIVTRRLSRTETSVAILFWSTLIVMGAGLVTAPFGWQPVTPTAAAWFVAAGVFNAA
                     AHFLLIEALRLGEAAVISPVRYTSLIWATLIGFLVWGDLPDAWVAAGSAVIIASGVYM
                     IRAEARKK"
     gene            5862..7283
                     /locus_tag="AC158Q_02765"
     CDS             5862..7283
                     /locus_tag="AC158Q_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HIR3338718.1"
                     /translation="MQITSMHFKATAGVKLRNANLQAALKRLQGNFVRGRADRVAELG
                     NYEEIRSAAAAIRDRALASLDLYLQEFERNATARGAVVHWAETREDVNRIVLEIARAH
                     GVKKAVKSKSMVTEECALNDALEAAGVEVVETDLGEYILQLAKEPPSHIVAPVVHKTR
                     DEVSDLFEEKHRTPRKTDIAELTREARERLRGHFITADMGITGANFIVAETGSTLIVT
                     NEGNGRMVTTLPRVHVAITGIEKVVPTLEDVATLMRLLPAHGTGQTISNYISVTTGVK
                     GGSDRDGPEHFHVILLDGGRSGLLGGDLQPMLRCIRCGACMNHCPVYQSVGGHAYGWV
                     YPGPMGSVLTPSYVGLENAPDLPNAATFCNQCGVVCPVKIPLPDLMRKLREQQFERGL
                     KPWTERAGLALWGWAVQRPSTYAMLATLGARVLSWMGGAEKRLRHIPFGAGWTAGRDL
                     PAPEGRTFRALYARSRRGSNDEG"
     gene            7303..8823
                     /locus_tag="AC158Q_02770"
     CDS             7303..8823
                     /locus_tag="AC158Q_02770"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004200059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HIR3338719.1"
                     /translation="MNKTLESRDAAVVPLWIGGRKQPATSKRSGDVTNPATGKVTRRV
                     PFCNAADVDAAVRAATAAFPGWRDTPPLRRARVMQKFLQLLQAGQKELARLVTEEHGK
                     TLPDAMGSVQRGIEVVEFACGIPHLLKGEHSENVGSGVDCHTVRQPVGVCAGVTPFNF
                     PVMVPLWMFPVAIACGNTFVLKPSEKVPSASMRMAELLKEAGLPDGVFNVVHGDKEAV
                     DAILNHPGIHAVSFVGSTPIAKYIYETCAKNAKRVQALGGAKNHAVVLPDADLEFAAD
                     ALIGAAYGSAGERCMAISAVVAVGAAADPLVKLLKKKAEAIAIGPGDKEGVDMGPLVT
                     AQHRDKVAGYIEQGVKEGARLVADGRGHKVPGYEGGNFLGTTLFDHVTPEMKVYQDEI
                     FGPVLVVLREKTLEDAIALVNRNPYANGTAIFTESGGAARRFENEIQVGMVGVNVPIP
                     VPVAFYSFGGWKGSLFGDLHVHGMEGVKFYTRTKVITSRWPHQDTPAPGLNMPTLG"
     gene            8858..9505
                     /locus_tag="AC158Q_02775"
     CDS             8858..9505
                     /locus_tag="AC158Q_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutC/YkgG family protein"
                     /protein_id="HIR3338720.1"
                     /translation="MTARENILARIRARQGKPAAALPGERETVRSHIEAHSASPRPRM
                     DWDTVARFRERALGLASTVEEVASLEAVPAAVASYLKSRSLPVTAVCWPGLAALPWGG
                     AGVEVEARAARDADRVGITGAFCAIADTGTLMTISGRDTPATASLLPETHIAVLGKSR
                     IVRGMEEAWQLARAELGRLPRAVSFISGPSRTADIEQTVTLGAHGPYRVHIILAG"
     gene            9686..>10888
                     /gene="glgB"
                     /locus_tag="AC158Q_02780"
     CDS             9686..>10888
                     /gene="glgB"
                     /locus_tag="AC158Q_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HIR3338721.1"
                     /translation="MHAKKTNVKANQDNGNSRAPGTADAFLTAHDIYLFREGTHARLY
                     EKLGAQCAAAKDGAVVHFAVWAPNARSVSVIGDFNGWDAAAHRLASRPDGSGIWEGAV
                     PGVRKGALYKYRIESHAGGSFEKADPFAFSAELPPSTASRVWTLDYEWGDAAWMKARG
                     ARNALDAPMTLYEVHAGSWRRKGGKFLEYRALARELADYVEKMGFTHVELMPVTEHPF
                     YGSWGYQTTGYFAPTARYGTPQELMYLVDTLHQRGIGVILDWVPSHFPDDPHGLARFD
                     GTHLYEHADPKQGYHPEWKSSIFNYGRNEVQAFLLSSALFWLDRYHFDGLRVDAVASM
                     LYLDYARKDGEWIPNRYGGKENLDAIRFLRMLNEAVYRDHPDTVSIAEESTAWPMVSR
                     PTYLGGLGF"
BASE COUNT         1809 a         3445 c         3775 g         1859 t
ORIGIN      
        1 tgctcgcgag cggccgcagc cgcgcgccgg cacggccctg gctcggcgtc tattccgagg
       61 agatccaggg acacgtggtg gtgacgcgcg tgctgcccga atcacccgct gcgcaggcag
      121 gcctcgcccg cggcgatatc atcctcgggg tggggggcga ggcggtcggg cgccagagcg
      181 agttttatca acgcctgtgg tcgagcggag atgccgggtc gccggtcatc ctgcacgtgc
      241 tgcacaagcg gacggtgcgc cagctcacag tgcgttccat ggaccggatg gctttcctgc
      301 gtccctggca ggcctagctt gaaaggcgcg agtcatgatg cgtgattcga attgatttcc
      361 ctcaccccgg gtccggttga gcgggtgcgg acgagggcgc tcgattgacc aatcaggcat
      421 ttccgcccct gggcgcgtag tcgtccggcg cgccgggggg tatgggcgga tgggctttca
      481 ggccttcctg gaattcgtgg atcaggcgcc gcatcggcgt gcgcacccag ctcgaaacca
      541 cgtgcaggtc ggtttcttcc ttcgggtcct ggatgacgtt gaagatcagc ggtgactcga
      601 ggtggttcgg ccccgcgttg ggttccgctt cccacaccag gtgcattttc cagtcgcgcc
      661 acttgaccgc gcgcagctcg ttcttgatgt agtaaacgaa tccctcccgc ccggattttt
      721 cactgctgcc aagaaaaaaa tcgagctggt ccacgccgtc gatcggacgg tcggtcggaa
      781 gggccgcccc cgcaacgcgc gcgagcgtcg tgaaaagatc cgttacgtgc acgatttcat
      841 tgctcacgcg gcccggtggc acgcgtcctg gccagcggat catgaacggc gcgcgcaggc
      901 ccccttccat cgcggtgtga taggtgccgg tccacgggcc ggcggtgccg cgccacgggc
      961 gccggaattc gggaccgttg tcgctcgcaa agatgaccag ggagtcgccg gcaatcttca
     1021 aggcatccat ttcatccagt acctggccga cgcggtgatc catttccgcc attgaatcgg
     1081 cgaaatcgcc ggcgccggtg cggccggcaa aatcccggtg cggcagcgtg gggtagtgca
     1141 gctgcgtgat gggaacgtag gcaaagaacg gctttccggc agcggcctgc ctgcgcatga
     1201 agtcgcaggc gcgcgcggcg aggtccgagt ctatgcgccg gcgcatctcc aggtcgtaga
     1261 ccgcaacgtt gcgcgcgggc ccgcccttgc ggccttccat cacgtagggc agcggcacgg
     1321 cctgcgggtc gtacccgggc gaactggtga acatgctctc attggtggtg cgcgggatgc
     1381 cgtaccactc gtcaaagccg cggtcgatgg ggaaacgccc cgtgcggtcg ccgaggtgcc
     1441 acttgccgaa gatggccgtc gcgtagccct tgccggacaa caactgcgcg agagtgattt
     1501 cccacggcgt gattccctgt ggcaggccgg cgggcactgc ctgcagcgcg ccggtgcgaa
     1561 tcgggtgccg gcccgtcatc agcgccgagc gcgtcggcac gcagtcgctt tcgacgttga
     1621 agttggtgag ccgcagccct tcggtggcga gtttatcgat acgcggggtc ggcgcgccgc
     1681 gcaggacgcc gccgccgtag cagcccagtt cgccccagcc gagattgtcg gccaggatca
     1741 gaacaatatt cggcgtttgc ggattgcggt gttgcgccat gcggatcttg aggcagggtg
     1801 gctggaccga atcctagcac ggtggccggc gcgagcgccg ctcccggagg cgacgggagc
     1861 aaggcgcatt ttctgcgata atgtcccgca cgccaggtgc ttcgcgcgaa gcgcgggcag
     1921 ccacacccac ttcacgattt cccgaaagtc gatgcgcgtc ggactcttcg tcacctgtct
     1981 tgtcgacctg atgcggcctc ggatcggatt ttctgcgatc cggctgctcg aggcggcggg
     2041 ttgcgacgtc gtcgtaccgc aggggcaaac ctgttgcggc cagcctgcct ataactcggg
     2101 cgatcgggca accgcccgtt cgctggcgct caaggtgctg gacgaatttg agggttgcga
     2161 gtacgtggtg gtcccctccg gctcgtgcgc cggcatgatc cgcgcgcatt tccccggtct
     2221 ttttaccgct gagccggccg aactcggtcg ctgcagggaa ctggccgcgc gcacatacga
     2281 gttgaccgat ttcctggcga atgtcgcgaa actccagcaa gtgccgggaa cattccatgg
     2341 aaccgtgacc tatcatgatt cgtgctcggg tttgcgcgag ctgggcgtga aatcgcagcc
     2401 gcgcgcgctg ctggcgaagg tgccgggact cgcgctgaag gaaatgaacg aagccgagac
     2461 ctgctgcggc ttcggcggcg cctttgccgt caagttcggg gagatatccg cgcatatcgc
     2521 cgagcgcaaa tgcgcgaaca tcgccgagtc cggcgcggac gccgtggtac tgggcgatct
     2581 cggctgcatg ttgaacatcg aaggccggct gcgccggcgc ggcgacatga aaacgaaagt
     2641 cctgcacgtc gcagaagtgc tggcaggaaa aatagaatga accgccaagg ccgccgggaa
     2701 gagcgaatta tttccaggat ccagagccag actatctggc gctggaaagt caaatacaaa
     2761 tatttcccgc ggagtaattg atcttgattc cagagttcat tgtcttgtca ggtttcttgg
     2821 cggccttggc gtcttggcgg ttggataatc tggacaactg caagtgaaga tagcagtcat
     2881 gggctcgggc ggcgtcggcg gtttctacgg cggccgcctg gcgcacacgg ggtgcgacgt
     2941 cacgttcgta gcgcgcggcg cgcacctcaa ggccatgcgg gagagcggcc tgacgatcga
     3001 aaacgaggcg caggggaaca tccacctttc cagggtcaaa gtcgtggagg atcctgccgc
     3061 aatcggcccg gtggatctcg tgatcatcgc ggtgaagctg tgggacaccg aaagtgccgc
     3121 gcgtgccgtg aagcccatcg tgggtccgga caccgccgtc ctgtcgcttc agaacggcgt
     3181 gatcaaggac gacatcctga ggcgcgaatt cggcgaccgg gccgtcatgg ggggcgtcgc
     3241 ctatgtcggg acgaatattg cccgccccgg cgtcatccgc cagatcggca cgctgcaacg
     3301 cctgatattc ggagaatacg acggccggcg ctcggctcgc gccgaagcgc tactcgccgc
     3361 cctgctgcgc gccggcatcc aggccgaatt gtccggcgac atacgcagga cgctgtggga
     3421 aaaatattcc tttctcgtcg ggctgtcggc gacgaccgcc gccatgcggg tgccgatcgg
     3481 cgcgattcgc tccaatccgc agacgcgcgc gttcctgttc gatatcctga aggaaacggt
     3541 ggccgtaggc cgcgcgctcg gcgtcgcgct gccggagaac tacgccgaag accgcctcgc
     3601 cttcgcggac agcgttcccg cggacatgac gtcctccctg caccacgacc tcgagcgcgg
     3661 caatccgctc gaggtcgagt ggctgtccgg cggcgtcgtg cagctgggga agcaggccgg
     3721 cgtgcccacg ccgatgaacc gcgcggtctg ggacatcctc gccttgcatg ctcgaggcag
     3781 gaaggtgtga atcacgcttt tcgagcatga acgcgccacg ccttacggtt cgtaatctcc
     3841 gggcgacgcc ggtgctggtg ccgatgacct acgtcctcgg caccagcgcg caggcggtgc
     3901 gcgaggcgcc gctgctgctg gtggaccttg aaaccgagga gggcgtgacc gggcacgcct
     3961 accagttttg ctatcttccg gccgcggcgc ccgcgatcat gattctgctc gacgaagtgc
     4021 tgcgcacggt agcaggcgat cgcgcggagc cggttgcact ctgggagaag ctcacgaagc
     4081 gcttcacgtt gatcggcgtg cagggcgtcg tgcgcatggc gatggccatg ctcgacgtcg
     4141 cgtgctggga cgcgctcgcg aaggcggccg ggaaaccgct ggcagcgttc ctcggcggct
     4201 cgcccggggc ggtgccggcc tacaacagct gcggcctggg cctgatggcg ccggcggccg
     4261 tggcggacga ggcggaacag ctgctcgccg ggggctttcg cgcggtgaaa ctgcgcctgg
     4321 gttacccgac gccggaagcc gatctcgccg cggtgcgcgc ggtgcgcaac cgcatacccg
     4381 atgacgtggc attgatggtt gattacaacc aggccttgac cgtgcaggag gcgatccggc
     4441 gcggccgcgc gctcgacggc gagggcgtct actggatcga ggagccggtc cgtcacgacg
     4501 atttcaccgg ctgcgccgaa atcgcacgcg cgctttcgac gccggtgcag atcggcgaga
     4561 acttctcgca ggtccacgac atggagcgcg cgctggcggc gcaggcctgc gattacgtga
     4621 tgcccgacct cgaacgcatc ggcggggtca ccggctggcg gcgggcgggt gagctggccg
     4681 gcgcgcggag aatcaggatg tcctcccatt tgtacccgga agtcagcgcg catctcctgg
     4741 cggcgacgcc ggcggcgcac tggctcgaat acgtcgattg ggcgaatgcc gtactcgccg
     4801 agccgttgag gatttccgac ggtcatgcgg tcgtctcggg gcatccgggc aacgggctcg
     4861 cctggaacgc ggacgccgtc cgcagattcc ggatgaactg agcgaccggc cgcacgcggt
     4921 gtccaatacg ctcaaaggca tcgtggtcat ggccgtcggc gttgccctgc tcacggcgaa
     4981 cgacgcgatt tccaagtacc tcacggagtc ccacccggtg gggcaggtga tctgcctgag
     5041 acaggcggcc acgctgctcg tgatcgtgcc gtacgtgatg ctggttaccg gctggggcgc
     5101 gctgcgcgtg gtctcgtggc cgggacaact gacgcggggg ctgctgtttg tcgccaatgc
     5161 cgcgctgatc gtgctgggct tctccctgct gccgctggcg acggtgatca cgatcatgtt
     5221 ctcgagcccg atcttccttg cgctgctctc cgcgtcgctg ctgacggaac gggtcaccct
     5281 cgatcgctgg atcgcaatca tcggcggatt tgccggcgtg ctgatcgtca taaggccggg
     5341 cgctacggcg ttcgactggg tgctgctgat acccgtgggc gcggcgctcg ccaatgcgct
     5401 gagggacatc gtgacgcggc ggctctcccg caccgagact tcggtcgcga tcctgttctg
     5461 gtccacgctg atcgtgatgg gtgcgggact cgtcaccgcg ccgttcggtt ggcaaccggt
     5521 gacgcccacg gccgcggcct ggttcgtggc cgcgggggtc ttcaatgccg cggcgcattt
     5581 tctgctgatc gaggcgctga ggctgggaga agcggcggtc atctccccgg tgcgctacac
     5641 gtccctcatc tgggcgacgc tgatcggctt tctggtgtgg ggcgacctgc ccgatgcctg
     5701 ggtggcggcg gggtccgccg tgatcatcgc gagcggtgtc tacatgatcc gcgccgaggc
     5761 gaggaagaaa taggccacca gcggcggaat cacgcagaag ctgaggcgtt gcccacgtta
     5821 taatccgggc gcgctacggc tttccatccg gctcccggat catgcagatc acttccatgc
     5881 acttcaaggc gaccgcgggc gtgaagctgc ggaacgccaa tctccaggcc gcgctgaaac
     5941 ggctccaggg caacttcgtg cgcggccgcg ctgatcgcgt tgcggagctc ggcaattacg
     6001 aggagatccg ctcggccgcc gcggcgatcc gcgaccgggc gctcgcgagc ctcgacctgt
     6061 acctgcagga gttcgagcgc aatgcgaccg cgcgcggcgc ggtggtgcac tgggcggaga
     6121 cgcgcgaaga cgtgaatcgc attgtcctgg aaatcgcccg cgcccacggc gtgaagaagg
     6181 cggttaagtc aaagtccatg gtgaccgagg aatgcgcgct gaacgacgcg ctcgaggcgg
     6241 cgggcgtcga agtggtcgaa accgacctcg gcgaatacat cctgcagctt gcgaaggaac
     6301 cgccctcgca catcgtcgcg ccggtggtgc acaagacccg cgacgaggta tctgatctct
     6361 tcgaagagaa acaccgcacc ccgcgcaaga cggacatcgc ggaactgacg cgcgaggcgc
     6421 gcgagcggct gcgcggccat ttcatcacgg ccgacatggg cattacgggc gccaacttca
     6481 tcgtggccga gaccggctcc acgctgatcg taaccaacga gggcaacggc cgcatggtga
     6541 cgacgctgcc gcgcgtgcac gtggcgatca ccggcatcga gaaagtggtg ccgacgctcg
     6601 aggacgtggc gacgctgatg cgcctgctgc cggcgcacgg caccggccag accatctcca
     6661 actacatttc cgtcacgacc ggcgtgaaag gcgggagcga tcgcgacggg cccgagcatt
     6721 ttcacgtgat tctcctggac ggcggccgca gcgggctcct cggcggcgac ttgcagccga
     6781 tgctgcgctg catccgctgc ggcgcgtgca tgaaccactg cccggtgtac cagagcgtgg
     6841 gcggccacgc ctacggctgg gtctatcccg ggccgatggg ctcggtgctg acgccgagtt
     6901 acgtggggct ggaaaacgcg cccgacctgc ccaatgccgc caccttctgc aaccagtgcg
     6961 gcgtcgtgtg ccccgtgaag atcccgctgc ccgatctcat gcgcaagctg cgcgagcagc
     7021 agttcgagcg cgggctcaag ccgtggacgg agcgggcagg actcgcgcta tggggttggg
     7081 cggtgcagcg tccctcgacc tatgcgatgc tggccaccct cggcgcgcgc gtgctgtcct
     7141 ggatgggcgg ggccgaaaag cggttgcgcc atattccatt cggcgcaggc tggaccgcgg
     7201 gacgcgatct gccggcgccg gaaggccgca cgttccgcgc gctttacgcg cgttcgcgtc
     7261 gaggctccaa tgatgaagga tgaatgatga atggagtctg tcatgaataa aactcttgag
     7321 tcccgggacg cggcggtcgt gccgctatgg atcggcggca ggaaacagcc ggccacgagc
     7381 aaacgctcgg gcgacgtgac caatcccgcg acgggcaagg tgacccggcg ggtgccgttc
     7441 tgcaacgcgg ccgacgttga tgcggcagtc agggcggcga cggcggcgtt tcccgggtgg
     7501 cgcgacacgc cgccgttgcg gcgcgcgcgc gtgatgcaga aattcctgca gttgctgcag
     7561 gccggccaga aggaactggc gcggctcgtc acggaggagc acggcaagac cctgcccgat
     7621 gcgatgggtt cggtgcagcg cggcatcgag gtggtcgagt tcgcctgcgg cattccgcat
     7681 ttgctgaagg gcgagcactc cgagaacgtc ggttccggcg tggattgtca tacggtgcgc
     7741 cagccggtcg gcgtgtgcgc cggcgtgacg ccgttcaact tcccggtcat ggtgccgctg
     7801 tggatgttcc cggtggcgat cgcctgcggc aatactttcg tgctgaagcc ctcggagaaa
     7861 gtgccctcgg cctcgatgcg catggcggag ctcctcaagg aggccggtct tcccgacggc
     7921 gtgttcaacg tcgtgcacgg cgacaaggag gcggtggacg cgatcctcaa ccaccccggt
     7981 atccacgcgg tttctttcgt gggctccacg ccgatcgcga agtacattta cgagacctgc
     8041 gccaagaacg cgaagcgcgt gcaggcgctg ggcggggcga agaaccacgc ggtggtgctg
     8101 cccgacgccg atctcgagtt tgccgccgac gcgctgatcg gcgcggccta cggctcggcg
     8161 ggcgagcgct gcatggcgat ctcggcggtg gtcgcggtgg gtgcggccgc cgatccactg
     8221 gtcaagctgc tgaaaaagaa agcggaggcg atcgcaatcg gcccgggcga caaggagggc
     8281 gtcgacatgg gcccactcgt gaccgcgcaa caccgtgaca aggtcgcggg ctacatcgag
     8341 cagggcgtga aagagggcgc gcggctggtc gcggacggcc gcggccacaa ggtgcccggc
     8401 tacgagggcg gaaatttcct cggcaccacg ctgttcgacc acgtgacccc ggagatgaag
     8461 gtctaccagg acgaaatctt cgggccggtg ctggtcgtgc tgcgcgagaa gacgctcgag
     8521 gacgcgattg cgcttgtcaa ccgcaacccc tacgccaatg gcacggcgat tttcaccgag
     8581 tcgggcggcg cggcgcggcg cttcgagaac gagatccagg tcggcatggt cggcgtgaac
     8641 gtgccgatcc cggtgccggt ggcgttctat tccttcggcg gctggaaggg ctcgctcttc
     8701 ggcgatttgc acgtgcacgg catggagggc gtcaagttct atacccgcac caaggtcatc
     8761 accagccgct ggccgcatca ggatacgccg gcgccgggct tgaacatgcc gacgctgggc
     8821 taacgccaag gcgaaaggcg aaagtcggaa ggcgaaagtg actgcgcggg aaaacatact
     8881 tgcgcgcata cgcgcgcgac agggcaagcc ggcagcagca ttacctggcg agcgcgagac
     8941 ggtgcgttcg catatcgaag cgcattccgc cagcccgcga ccacgcatgg actgggatac
     9001 ggtcgcgcgt tttcgcgaac gggcgctcgg gctggcgagc acggtggaag aagtggcgtc
     9061 gctcgaggcg gtgccggccg ccgttgcctc ctacctgaaa tcgcgctcgc tgcccgttac
     9121 ggcggtatgc tggcccggac tcgcagcgct tccatggggt ggcgcgggtg tcgaagtcga
     9181 agcgcgcgcg gcgcgcgacg ccgatcgggt agggatcacc ggggcgtttt gcgccatcgc
     9241 cgacaccggc accctgatga cgatatcggg gcgcgacacg ccggccaccg ccagcctgtt
     9301 gccggagacg catattgccg tgctggggaa gtcgcgcatc gttcgcggca tggaagaagc
     9361 gtggcagctc gcgcgcgcgg agctcgggcg gctgccgcgc gccgtcagtt tcatctcggg
     9421 cccatcgcgc accgccgaca tcgagcagac cgtcacgctc ggcgcgcacg ggccctaccg
     9481 cgtgcacatc attctcgcgg gctagtcggg gtggcgctgc ggcgcgtcag gccggtgcgg
     9541 cgcatccgca agccatcgtc atatcgcttc cggatcaacg gcttatggcg gcagttccgc
     9601 gcggaacggg aacttttcat gaccgggtga gacacaccat aatgacgccg gcaggtgcgt
     9661 ttctcccgaa tcgcgtcgag gcgcgatgca cgcgaaaaaa acgaacgtca aagcgaatca
     9721 ggataatggg aactcccgcg cgcctggcac ggcggacgcg ttcctgaccg cgcacgacat
     9781 ctatcttttc agggaaggca cgcacgcgcg cctctacgag aaactcgggg cgcagtgcgc
     9841 ggcagcgaaa gatggcgccg tggtccattt cgcggtttgg gcgccgaatg cccgctccgt
     9901 ttcggtgatc ggtgatttca acggctggga tgctgccgcg caccggctgg catcgcggcc
     9961 cgacggctcg ggcatctggg agggcgccgt tcctggcgtc cgcaaagggg cgctctacaa
    10021 gtaccgtatc gagtcgcacg cgggcggcag cttcgagaag gccgatccgt tcgccttcag
    10081 cgccgagctg ccgccctcca ccgcttctcg cgtgtggacg ctcgactacg aatggggcga
    10141 cgcagcgtgg atgaaggcgc gcggcgcgag gaacgcgctg gacgcgccga tgaccctcta
    10201 cgaggtccac gccggctcat ggcggcgcaa gggcggcaaa ttcctcgagt accgggcgct
    10261 cgcgcgcgag ctcgcggact acgtggagaa gatgggtttc acccacgtgg agctgatgcc
    10321 cgtcaccgag cacccgttct acggctcctg gggctaccag accacaggct atttcgcgcc
    10381 caccgcgcgc tacgggacgc cccaggaact gatgtacctc gtggacaccc tgcaccagcg
    10441 cggcatcggc gtgatactcg actgggtgcc ttcgcacttc cccgacgatc ctcacgggct
    10501 cgcgcgcttc gacggcacgc acctgtacga gcacgccgat ccgaagcagg gttatcaccc
    10561 ggaatggaaa tcgagcatct tcaactacgg gcgcaacgag gtccaggcgt tcctgctgtc
    10621 ttccgcgctg ttctggctcg atcgctacca cttcgacggc ctgcgcgtgg acgcggtcgc
    10681 gtcgatgctg tacctggact acgcgcgcaa ggacggtgag tggatcccga atcgctacgg
    10741 cggcaaggag aacctcgacg ccatccgctt cctgaggatg ctcaacgagg cggtgtaccg
    10801 cgatcatccg gatacggtga gcatcgccga ggagtcgacg gcctggccga tggtttcgcg
    10861 gccaacctac ctgggcggac tgggtttc
//
LOCUS       DBNGSG010000108         6428 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2922657, whole genome shotgun sequence.
ACCESSION   DBNGSG010000108 DBNGSG010000000
VERSION     DBNGSG010000108.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6428)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6428)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6428
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2922657"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(211..477)
                     /locus_tag="AC158Q_02785"
     CDS             complement(211..477)
                     /locus_tag="AC158Q_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HIR3338722.1"
                     /translation="MIDDAGLLEYPCDFPVKILGHTQAGFAQAVLDVVKRHAPDFDGA
                     SMELRASKRRKYLSVTCVIRATSREQLDNLYRELCDHPMVVMVL"
     gene            complement(474..1334)
                     /locus_tag="AC158Q_02790"
     CDS             complement(474..1334)
                     /locus_tag="AC158Q_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HIR3338723.1"
                     /translation="MSDTVYLNGEFLPLEKAQVSVLDRGFIFGDGVYEVIPVYSRRPF
                     RLPEHLARLQHSFDAIRLGNPMTDAEWTRLILELVGRNAAEDQSVYLQVTRGVAKRDH
                     AFPRDSKPTVFMMSGPLVTPTKEQVESGVSCITATDFRWLKCDVKSISLLGNCLLRQS
                     AADAGAAEVVLFRDGYLTEASASNVFVVRDGRLLAPPKNHLILPGITYDFVLELAQAC
                     GLPIELREISEQEVKSADEIWVTSSTKEVLAVTALDGRAVGSGRPGEAFRVVHREYQE
                     YKRKVMRQAA"
     gene            complement(1392..2528)
                     /locus_tag="AC158Q_02795"
     CDS             complement(1392..2528)
                     /locus_tag="AC158Q_02795"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR3338724.1"
                     /translation="MRSLLLLLLSVFPAALPAQQPAFAVPPAPAIAANAYLLLDFHSG
                     QVLAAREPHARVEPASLTKLMTAYVAFTALKQKRIRADQAVPVSERAYRTVGSRMFIE
                     AGKPVTVYELVRGMIVQSANDACIALAEAIAGSEEAFAEMMNQEARRMGLANTHFVNS
                     TGLPHPQHYSTAQDIAALAAAVIRDFPEHYPLYSQKEYRYNGVAQSNRNRLLWTDPTV
                     DGMKSGYTAYAGYCLVTSARRGERRLVAVVLGAASETARAAESQKLLNHGFQAYDGVR
                     LYSSNQPVATFAVYKGSANSIRAGFITGLQLALPRGSGDKLKATIESMEPLLAPIRAG
                     QRVGTLKLTLDDKPYRELPVVALDDVPVAGILGRGWDTLRLLFR"
     gene            2788..3213
                     /locus_tag="AC158Q_02800"
     CDS             2788..3213
                     /locus_tag="AC158Q_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR3338725.1"
                     /translation="MANITRFNAFDDSLDDLFRGFFVRPVSYDAPSGAAQFRVDVSEN
                     DTAYTLRAEIPGVKKDEINVSIDGDTVAISAEVKNEKDVKNGDRVLRSERYYGKVYRA
                     FTLGQAVDEASAAARYADGVLELTLPKKAAVQAKRITIQ"
     gene            3383..4270
                     /gene="argB"
                     /locus_tag="AC158Q_02805"
     CDS             3383..4270
                     /gene="argB"
                     /locus_tag="AC158Q_02805"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HIR3338726.1"
                     /translation="MALTGNTAAEKAKILAEALPYIRRFHGKTIVIKYGGNAMTDDKL
                     KNGFAHDVVLLKLVGINPVVVHGGGPQIEQLLARLGKKGEFVQGMRVTDAETMDVVEM
                     VLGGQVNAEIVNLINQHGGRAVGLTGKDGGLIRAKKMLVRGNGGSEEMIDIGQVGEVD
                     TIDPEIVHHLLTNDFIPVIAPLGVGKKGESYNINADLVAGKMAEVLKAEKLIVLTNTP
                     GVLDKNGNLLTGLSSKKVDELFANGTISGGMLPKISSALDAVKNGVNTCHIIDGRVEH
                     ALLLEVLTDEGVGTLIRRR"
     gene            4293..4937
                     /locus_tag="AC158Q_02810"
     CDS             4293..4937
                     /locus_tag="AC158Q_02810"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HIR3338727.1"
                     /translation="MQFNKVWIFDLDNTLHNATPHIFPHINRAMTAYLQAHLGLTEDA
                     AGELRRHYWKRYGATLLGLMRHHQTDPRHFLWQTHQFPELDRMLVREPQLRGTLTRLP
                     GRKIVFSNSPVHYSSAVLKALGIADLFDDVFSIERTRFRPKPDSFGFLRLCRANRVKP
                     QRCIMVEDSLDNLRTAGRLGMKTVWVTQEARAPGCVDVKVANLMQLPRLLSYLQ"
     gene            4964..5551
                     /gene="slmA"
                     /locus_tag="AC158Q_02815"
     CDS             4964..5551
                     /gene="slmA"
                     /locus_tag="AC158Q_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HIR3338728.1"
                     /translation="MAEKTGERKDQILQTLAQMLENPTGEKVTTAALAARIKVSEAAL
                     YRHFKGKAEMFEGLIEFIEQALFGLINRITSDEKSGVRQVEGIISVLLAFAQKNRGMT
                     RVLIGDALVNEDDRLQQRINQLHERLEAALKQALRFGVSQKEIAQEVDLTAQANLMMS
                     FVTGRWHQFAKSGFRRDPIEMWQKQWRQLMEGVIA"
     gene            5708..6064
                     /locus_tag="AC158Q_02820"
     CDS             5708..6064
                     /locus_tag="AC158Q_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338729.1"
                     /translation="MANITTQQLAEIIIGVARSQQAIIDAVDSQKTGFKGSHLAPALD
                     NAAKSRVTTRPLTLQEFPARVLLQCMGRAGPNVEQLTRDLEALLSGQTVTPAAPAATP
                     AAPATAADGAQSLDMT"
     gene            complement(6156..>6428)
                     /locus_tag="AC158Q_02825"
     CDS             complement(6156..>6428)
                     /locus_tag="AC158Q_02825"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HIR3338730.1"
                     /translation="VFDLRVSGAPCYSCLFPEHGESEDVRCAVMGVFAPLTGVIGSIQ
                     AAEALKVLTGSGETLNGRLLLLDALAMQVRVIRLAKDSACTVCSTV"
BASE COUNT         1266 a         2022 c         2084 g         1056 t
ORIGIN      
        1 cggcgcaatg gctgttatcc cggctgcacg accgccagat cgcggcgcta aaacctggaa
       61 attagccacc aagacgccaa gggcgccaag aaaatctttc aatcagccgc agaggcgcaa
      121 aggcgcagag aagaacggga ataaaaccgc cgatgaacgc agataatcca ttcactattc
      181 accgttcacc attcacgcgg ttcgggtgtt ttagagcacc atgaccacca tcgggtggtc
      241 gcagagttcc cggtagaggt tgtcgagttg ctcgcgcgaa gtggcgcgga tcacgcaggt
      301 gacgctcagg tacttgcggc gcttgctggc cctcagttcc atggaggcgc cgtcgaaatc
      361 cggcgcgtgg cgcttgacga cgtcgagcac ggcttgcgcg aaccccgcct gcgtgtgacc
      421 cagtatcttg accgggaaat cacagggata ttcgagcagc ccggcgtcgt cgatcatgcc
      481 gcttgacgca tcaccttgcg cttgtattcc tggtactccc ggtgcaccac ccggaatgcc
      541 tcgccgggcc ggccggagcc gaccgccctg ccgtcgagcg ccgtgaccgc cagcacttcc
      601 ttggtcgacg aggtcaccca gatctcgtcg gcgctcttca cctcctgctc cgagatttcg
      661 cgcagctcga tgggcaggcc gcacgcttgc gcgagctcga gcacgaagtc gtacgtgatg
      721 ccgggcagga tcaggtggtt tttcggcggc gcgagcaggc ggccgtcccg cacgacgaag
      781 acgttgctcg ccgaagcctc ggtgagatac ccgtcgcgga acagcacgac ttcggccgcg
      841 ccggcatcgg ccgcggactg ccgcagcagg cagttgccca gaagggatat cgacttcacg
      901 tcgcacttga gccagcggaa gtcggtcgcg gttatgcagg acacgccgct ttcgacctgc
      961 tccttcgtgg gggtcaccag gggacccgac atcatgaaca cggtgggttt cgaatccctg
     1021 gggaaggcat ggtcgcgctt cgcgacaccg cgcgtgacct gcaggtagac cgactggtcc
     1081 tcggccgcgt tgcggccgac aagctcaaga atcagccgcg tccattccgc atcggtcatc
     1141 gggttgccta gccgtatcgc gtcgaagctg tgctgcagcc gcgcgaggtg ctcgggcagc
     1201 cggaacgggc ggcgcgagta gacggggatc acttcgtaga cgccgtcacc gaagatgaag
     1261 ccgcgatcaa gcaccgagac ctgcgctttt tcgaggggca ggaattcgcc gttgagatag
     1321 accgtatcgc tcatcctggg cctctggagg tggcggcggg aagggggcta agtctatacc
     1381 ggaaaacccg gctagcggaa cagcagccgc agcgtgtccc agccgcgccc cagtatgccg
     1441 gcgaccggga cgtcgtccag cgccacgacc ggcaattccc ggtacggctt gtcgtccagc
     1501 gtgagcttca acgtgccgac tctctggccc gcgcggatcg gcgcgagcag cggctccatg
     1561 ctctcgatgg tcgccttcag cttgtcgccg cttcccctgg gcagggcgag ttgcaggccg
     1621 gtgatgaaac ccgcccgaat gctgttggcg gaacccttgt acacggcgaa ggtcgcgacc
     1681 ggctggttgc tggaataaag tcgcaccccg tcgtaggcct ggaaaccgtg attgagcagc
     1741 ttctggctct cggccgcgcg cgccgtctcg ctcgccgcgc cgagcacgac tgcgacgagc
     1801 cgccgctcgc cgcgccgcgc cgaggtgacc aggcagtagc cggcgtacgc ggtatagccg
     1861 gacttcatgc cgtccaccgt gggatcggtc cacaacaggc ggttgcggtt cgattgcgcg
     1921 acgccgttgt aacggtattc cttctgggag tagagcgggt agtgctccgg aaaatcgcgg
     1981 atcaccgccg ccgcgagcgc ggcgatgtcc tgcgctgtcg aatagtgctg aggatgcggc
     2041 aggccggtcg aattgacgaa atgagtgttc gcgagcccca tccgcctggc ctcctggttc
     2101 atcatttcag cgaaagcttc ctcgctgccg gcaattgcct cggccagcgc gatgcaggca
     2161 tcgttggcgg actgcacgat catgccccgg accaattcgt atacggtcac cggtttccct
     2221 gcctcgatga acatgcgcga gcccacggtg cggtacgctc gctccgaaac cggcaccgcc
     2281 tggtcggcgc gaatgcgttt ctgcttgaga gccgtgaagg ccacgtaagc cgtcatcagc
     2341 ttggtgagcg aggccggttc cacccgcgcg tgcggttcgc gggcggcaag cacctggccg
     2401 ctgtggaaat cgagcagcag ataggcattg gccgcgatgg cgggcgcggg cgggaccgca
     2461 aatgcgggtt gctgggctgg aagcgctgcg ggaaaaacgg acaacaggag cagcagaagc
     2521 gaacgcatgg ttttcaacga aagcggcgat tataacaaag cgggttccgg tccctcgccg
     2581 tggttgcgtg gcgggggcgc gaacaggagc gcggaagcgg agcggcgccc cgacccggcg
     2641 gccttgacgc attgccacag tgtcacaaaa aattcaggaa aatcagacca ttgttttcag
     2701 acatgcgcgg gtcttgaaaa cccgcgggcc ggctcccata tgtccgttgt cggcgcggac
     2761 agcgccccat ccaacatgga gacgagcatg gcaaacatta cccgtttcaa tgcgtttgat
     2821 gattccctgg acgacctgtt ccgtggcttc tttgtgcgcc cggtgagcta tgacgccccg
     2881 tccggcgccg cgcagttccg cgtggacgtg tccgagaacg acactgccta tacgctgcgc
     2941 gccgagatcc cgggcgtgaa gaaggacgaa atcaacgtgt cgatcgacgg cgacaccgtc
     3001 gcgatcagcg cggaagtgaa aaacgagaag gacgtgaaga acggcgaccg cgtgctgcgc
     3061 tccgagcgct attacggcaa ggtctatcgc gcgttcacgc tcggccaggc ggttgacgaa
     3121 gcctcggctg ccgcccggta tgccgacggc gtgctcgagc tgacgctgcc gaaaaaggcc
     3181 gcggtgcagg cgaagcgcat caccatccaa taaatccgga cagctgacgc tgccggttta
     3241 tttccgcgcg tttgaacgga tggggcaggg cccaccgctc aacgcgcggg acaaggaagc
     3301 gaggcggcgg gccgaaaggc ctgccgtttt tttttgcctt cccgtagaat agccggcgac
     3361 ccacactatt gatggcccga ccatggccct gaccggcaac accgccgcgg aaaaagcgaa
     3421 gatcctcgcc gaggcgctgc cgtacatccg gcgctttcac ggcaagacga tcgtcatcaa
     3481 gtacggcggc aacgcgatga ccgacgacaa gctgaagaac ggcttcgcgc acgacgtcgt
     3541 gctgctcaag ctcgttggca tcaacccggt ggtggtgcat ggcggggggc cgcagatcga
     3601 gcagctgctc gcgcgcctgg gcaagaaagg cgagttcgtc cagggcatgc gggtgacgga
     3661 tgccgagacc atggacgtcg tcgagatggt gctcggcggc caggtcaacg cggaaatcgt
     3721 caatctcatc aaccagcacg gcggccgcgc ggtcgggctg accggcaagg acggggggct
     3781 catccgggcc aagaagatgc tggtgcgcgg caacggcggc tccgaggaga tgatcgacat
     3841 cggccaggtg ggggaagtcg acaccataga tcccgagatc gtgcaccatc tgctcaccaa
     3901 cgatttcatt cccgtgattg cgccgctggg ggtgggcaag aagggcgagt cctacaacat
     3961 caatgccgac ctcgtcgcgg gcaagatggc cgaagtactc aaggccgaga aactgatcgt
     4021 gctcaccaac acgccgggcg tgctcgacaa gaacggcaat cttctcaccg ggctctcctc
     4081 gaagaaggtc gacgagctat tcgccaatgg caccatctcc ggcggcatgc tgcccaagat
     4141 cagctcggcg ctggacgcgg tgaagaacgg cgtcaacacc tgccacatca tcgacgggcg
     4201 cgtcgagcac gcgctgctgc tcgaggtcct taccgacgaa ggcgtcggca cgctcatcag
     4261 gcgccgctga gcgtcggcag gcggggcgga cgttgcagtt caacaaggtc tggatcttcg
     4321 acctcgataa caccctgcac aacgccaccc cgcacatctt cccgcacatc aaccgggcga
     4381 tgacggcgta cctccaggcg cacctgggcc tcaccgaaga cgcggccggc gagctgcggc
     4441 ggcattactg gaagcgctac ggggcgacgc tgctgggtct catgcgccac catcaaaccg
     4501 acccccggca ctttctctgg cagacacacc agtttccgga gctcgaccgc atgctggtgc
     4561 gcgagcctca gctgcggggg acgctcaccc ggcttccggg cagaaagatc gttttctcga
     4621 attcgccggt gcattattca agcgcggtgc tgaaggcgct ggggatcgcg gacctgttcg
     4681 acgacgtgtt ctcgatcgag cgcacgcgtt ttcgccccaa gcccgacagc ttcggcttcc
     4741 tgcgcctgtg ccgcgccaac cgtgtgaagc cgcaacgctg tatcatggtg gaggattctc
     4801 tcgacaacct ccgcaccgcc ggccggctcg gcatgaagac ggtgtgggtg acgcaggagg
     4861 cccgggcgcc gggttgcgtg gacgtgaaag tcgcgaatct catgcagttg ccccggctgc
     4921 tgtcctattt acaataggcg ccggagcccg aagcgaggcc ggaatggcgg aaaaaacagg
     4981 cgagcgcaag gaccagatcc tgcagacgct ggcgcagatg ctggagaacc cgaccggcga
     5041 gaaggtaacc acggccgcgc tcgccgcgcg catcaaggtg tcggaggccg cgctctaccg
     5101 tcatttcaag ggcaaggcgg agatgttcga gggcctgatc gaattcatcg agcaggccct
     5161 gttcgggctc atcaaccgga tcaccagcga cgagaagagc ggcgtgcgcc aggtggaggg
     5221 catcatctcg gtgctgctcg cgttcgcgca gaagaaccgc ggcatgacac gcgtgctgat
     5281 cggcgacgcg ctggtcaacg aggacgaccg gctgcagcag cgcatcaacc agctgcacga
     5341 gcggctggaa gcggcgctca agcaggcgct gcgtttcggt gtgagccaga aggaaatcgc
     5401 gcaggaagtg gacctcaccg cccaggccaa cctgatgatg agtttcgtca ccgggcgctg
     5461 gcaccagttc gcgaaaagcg gcttcaggcg cgacccgatc gagatgtggc aaaagcagtg
     5521 gcgccagctc atggaaggcg tcatcgctta ggggaacggg cgcgatcccc gttttcctaa
     5581 gcgaaggcgc ctgggcaagg aatgaacgag gggatgattt ccctcgtgct cccctacgtc
     5641 gggggcggta gcggcgcggc cgcaaccgcc aaaggggagc agcaacaaac ttaagggaga
     5701 tacaagcatg gccaatatca cgacccaaca gctagccgag atcatcatcg gcgtcgcccg
     5761 ctcgcagcag gcaattatcg atgcggtgga cagtcagaag acgggattca aaggcagcca
     5821 tctcgcgccg gcgctcgaca atgcggcgaa gagccgcgtc accacccggc cgctgacgct
     5881 gcaggaattt ccggcgcgcg tgctgttgca gtgcatgggg cgggccggcc ccaacgtcga
     5941 acagcttacc cgcgatctcg aagcgttatt gagcgggcag acggtaacgc cggcggcgcc
     6001 agcggccacg ccggccgctc ccgccaccgc cgcggacggc gcgcagtcgc tcgacatgac
     6061 ctgaacaaca gtcacaagtt acaagtttca aggtacaagt acgcaacgcg acggaacctc
     6121 gatctacttg aatcttgtga cttgtaactt gtaactcaaa ctgtcgagca gaccgtgcag
     6181 gccgaatctt tggccagcct gatgacgcgc acctgcatcg ccagcgcgtc gagcaacagc
     6241 agccgcccgt tgagcgtctc gcccgatccc gtcagcactt taagcgcttc cgcggcctgg
     6301 atcgagccga taacgcctgt cagcggcgcg aacacgccca tcaccgcgca gcgcacgtcc
     6361 tcggattccc cgtgctccgg gaacaggcag gagtagcagg gcgctccgct cacgcgcagg
     6421 tcgaatac
//
LOCUS       DBNGSG010000109         3343 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1564584, whole genome shotgun sequence.
ACCESSION   DBNGSG010000109 DBNGSG010000000
VERSION     DBNGSG010000109.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1564584"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="AC158Q_02830"
     CDS             complement(<1..992)
                     /locus_tag="AC158Q_02830"
                     /inference="COORDINATES: protein motif:HMM:NF025890.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR3338731.1"
                     /translation="MAKSKSRRIFWIWWGALTLTLGGYLSAGMLMKSAATNPLLAPAR
                     EFFLPGRTTYGHYQIELACESCHKSPFTGREGLQESCERCHGQELKDAVDKHPRSKFT
                     DPRNAELLEKLDATLCVTCHVEHRREITGPMGVTLAGDFCFHCHQDIAKDRPSHRGMA
                     FNTCNSAGCHKFHDNRALYEDFLAKHLGEPALLGKTSLPARNFRAIAADFPGYPADRY
                     PLEPQTVPDAPAQLMKSKDVAADWLATAHARSGVNCSGCHKAEREGEAAWVRHPDHKV
                     CTDCHEQEVKGFLAGKHGMRLAESLRPMTPAEARQPMHANSGHNALGCVSCHAA"
     gene            complement(996..2975)
                     /locus_tag="AC158Q_02835"
     CDS             complement(996..2975)
                     /locus_tag="AC158Q_02835"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3338732.1"
                     /translation="MPAIARAIESLPEAAEPIVVVGTGPVGVRVAQELARLAPRVPVV
                     IYGNEPSAPYNRVRLSSLLAGELNLNALVAGQDLPSGPQLTARLNCGITAIDRERRAV
                     VDAAGRAQPYWRLVLATGSRPLIPDIPGIRQDHVYVFRDLRDAERLCARRMRSRRTAV
                     IGGGLLGLEAARAMQRFNTEVCVVEHAARLMPRQLDDAGAACLASHVRRIGIQTILGD
                     PVRQVLGNGAVSGLRLRSGREIECDTLIVAAGIRPNIELAAAAGLGIGRGVRVNDFLQ
                     TSDPDIYAVGECAEHDGEVWGLIAPGLEQAAIAAHNLAGSRGRYRGSSVATRLKVLDL
                     PMFSMGAVNEYSRLDLASEFVHRDEAAGTYRKIVTERGRLIGAIALGEFPEMSRVQEM
                     ILQGRIVLPWQRWRFAREGTLWPEQEPGAVTEWPASAVVCNCTGVTRGEIARACAAGA
                     CTVEQVSLATRASTVCGSCRPLVAELAGAAGPAEPVRGWKVLLGTSSFALKTALVIAL
                     VIAVPYAATVQVPWQWDLLWRESFWKQASGFTVLGLTVLLVVMSLRKRIRRFTLGDFS
                     LWRLAHVVLGALTLAGLAVHTGGRLGSNLNFMLMASFLGVVALGGVAGGVIALEHRLG
                     AGAARLRRTWTWTHILIAWPIPVLLAFHVLKTYYF"
BASE COUNT          517 a         1159 c         1161 g          506 t
ORIGIN      
        1 tgcgcggcgt ggcagctcac gcatccaagg gcgttgtgac ccgaattcgc gtgcatcggc
       61 tggcgagcct cggctggggt catcggccgc agcgactccg caagccgcat gccgtgcttg
      121 ccggcgagaa accccttcac ttcctgctcg tggcagtcgg tgcacacctt gtgatcggga
      181 tgtcttaccc atgcggcttc gccttcgcgc tcggccttgt ggcagccgct gcagttcacg
      241 ccggagcgcg cgtgcgcggt cgcgagccag tcggcggcga cgtccttcga cttcatcagc
      301 tgcgccggcg cgtcgggcac ggtctgcggc tccaggggat agcggtccgc cgggtagccg
      361 ggaaaatccg cagcgattgc gcggaaattg cgcgcgggca gcgaggtttt tcccagcagc
      421 gcgggctcgc cgagatgctt ggcgaggaag tcctcgtaga gcgcgcggtt gtcgtggaac
      481 ttgtggcagc ccgcggagtt gcaggtattg aacgccatgc cccggtggct cgggcggtcc
      541 ttcgcgatgt cctggtggca gtggaagcag aagtctcccg cgagcgtgac gcccatcggc
      601 cccgtgatct cgcgccggtg ctcgacgtgg caggtgacgc acagcgtcgc gtcgagcttc
      661 tccagcagct cggcgttgcg cgggtcggtg aatttgctgc gcgggtgctt gtccaccgcg
      721 tccttcagct cctggccatg gcagcgctcg cacgactcct gcagcccctc gcggccggtg
      781 aaggggctct tgtggcagga ctcgcaggcg agctcgatct ggtagtgccc gtaagtagtc
      841 ctgcccggca ggaaaaactc gcgcgccggc gcgagcagcg ggttcgtcgc cgcgctcttc
      901 atcaacatcc ccgccgacag gtagccgccc agggtcaagg tcagcgctcc ccaccagatc
      961 cagaagatcc tgcggctctt ggatttggcc atgattcaga agtaataggt cttgagcaca
     1021 tgaaatgcga gcagaaccgg tatcggccag gcgatcagga tgtgcgtcca cgtccaggtg
     1081 cggcgcaggc gcgcggcgcc cgcgcccagg cggtgctcga gcgcgatcac gccgccggcg
     1141 acgccgccca gcgcgacgac gccgaggaaa gaggccatca gcatgaaatt gaggttcgag
     1201 cccagccgcc cgccggtgtg caccgcgagc cccgcgagcg tgagggcgcc cagcacgacg
     1261 tgcgcgaggc gccacagcga aaaatcgccg agcgtgaagc ggcggatgcg cttgcgcagc
     1321 gacatcacga cgagcagcac ggtcagcccc agcacggtga acccgctcgc ctgcttccag
     1381 aagctttcgc gccacagcag atcccactgc cacggcacct gcaccgtggc cgcgtacggc
     1441 acggcgatca cgagcgcgat cacgagcgcg gtcttgagcg cgaagctgct cgtgccgagc
     1501 agcaccttcc agccgcgcac cggttccgcc ggtcccgcgg cgcccgccaa ctcggcgacc
     1561 agcggccggc aggagccgca gaccgtggag gcgcgcgtgg cgagcgacac ctgctccacc
     1621 gtgcacgccc cggctgcgca cgcgcgagcg atctcgccgc gcgtcacgcc cgtgcagttg
     1681 cagacgaccg cgctggcggg ccactcggtg acggcgccgg gttcctgctc gggccacagc
     1741 gtgccttcgc gggcgaaacg ccagcgctgc cacggcagca cgatgcggcc ctgcaggatc
     1801 atctcctgca cgcggctcat ctccgggaat tcgccgagcg cgatggcgcc gatcagccgc
     1861 ccgcgctcgg tcacgatctt gcggtaggtg ccggcggcct cgtcgcgatg cacgaactcg
     1921 ctggcaaggt cgaggcggga gtactcgttg accgcgccca tgctgaacat cggcaggtcc
     1981 agcaccttca ggcgcgtggc gacgctcgag ccgcggtagc ggccgcgcga gccggcgaga
     2041 ttgtgcgccg cgatcgccgc ctgctcgagc cccggcgcaa tcaggcccca cacttcgccg
     2101 tcgtgctcgg cgcactcgcc caccgcgtag atgtcggggt cggaggtctg cagaaaatcg
     2161 ttcacgcgca cgccgcgccc gatcccgagg ccggccgccg ctgcgagctc gatgttgggg
     2221 cggatgcccg ccgcgacgat cagtgtgtcg cactcgatct cgcgcccgct gcgcagccgc
     2281 agccccgaca cggcgccgtt gccgagcacc tggcgcaccg gatcgcccag gatggtctgg
     2341 atgccgatcc tgcgcacgtg actggcaagg caggcggcgc cggcgtcgtc gagctggcgc
     2401 ggcatcagcc gcgcggcgtg ctcgaccacg cacacttccg tgttgaagcg ctgcatcgcg
     2461 cgcgcggcct cgagccccag cagtccgccg ccgatcaccg cggtgcggcg gctgcgcatg
     2521 cgccgcgcgc aaaggcgctc ggcgtcgcgc aggtcgcgaa acacgtaaac gtggtcctgg
     2581 cggatgcccg ggatgtcggg tatgagcggg cgcgagccgg tcgcgagcac cagcctccag
     2641 tacggctggg cgcgaccggc ggcgtcgacc accgcgcggc gctcgcgatc gatggcggtg
     2701 atgccgcagt tcaatcgagc ggtcaactgc gggcccgacg gcaggtcctg gcccgcgacg
     2761 agcgcgttca ggttgagttc gccggccagc agcgaggaca ggcgcacgcg gttgtacggc
     2821 gcggagggct cgttgccgta gatgacgacc ggcacccggg gcgcgagccg cgccagttcc
     2881 tgggcgacgc gcacgccgac cgggccggtg cccacgacga cgatgggctc cgctgcttcg
     2941 ggcaatgact cgatggcgcg cgcgattgcg ggcatggcga taccactccg caatcagcgt
     3001 gccattaccg cagcgtctgt gttttctcct tatataacct tgagttgaat cggagcgccc
     3061 cgcgctggag acctatttca aatgaataag gcgcactgtt gcggtgcaac atggtgtgcg
     3121 ggtgcactct tatggaaggg ggcctcgaaa ccggctgatt cgcgcggcga caccgcgggc
     3181 cgggcgtgca gcagcaaccg gatgacgggc taacagcact cctggtgccg ccgcttcatc
     3241 acgccgagca cgtcgtcggg ctcgcgcttc caccagttgc gcgccgagaa aatctcgacc
     3301 tcgtggaagc cgcgatagcc cgccgcttcc atccacgagc gga
//
LOCUS       DBNGSG010000110         3059 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1904231, whole genome shotgun sequence.
ACCESSION   DBNGSG010000110 DBNGSG010000000
VERSION     DBNGSG010000110.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1904231"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="AC158Q_02840"
     CDS             <1..478
                     /locus_tag="AC158Q_02840"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HIR3338733.1"
                     /translation="IAGELFNHLGKTNIAAVQYKGGGPALVAAISGEVSIAFSGVAGA
                     VGFVQSGRLRALGVTSRKPVAALPGVPPIAESGLPGYEFVAWFVLAAPKGTPREIIVM
                     LNEHFRKAMSFPDTIRRFEADGLDVVGSTPEQAAATMESELKKWAVVIKERGMRAN"
     gene            498..1748
                     /locus_tag="AC158Q_02845"
     CDS             498..1748
                     /locus_tag="AC158Q_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011003934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3338734.1"
                     /translation="MIEDGRGGVRETVDAVYRSESRRVFATLVRLLGDFDLAEEALHE
                     AFRAALEQWPRDGVPANPRAWLVSAGRFKAIDGMRRHARFDPLDEDAVEDLAAPDDPA
                     TWGAEEIEDDRLRLIFTCCHPALPPDAQVALTLREVCGLTTEEIAHAFLAAPPTLAQR
                     IVRAKTKIRAAKIPYQVPAPADLPERLDSMLRVIYLVFNEGYAASSGASLTRHDLSGE
                     AIRIAGLLVDLLPEPEAMGLYALMLLHESRRGARATPAGDLILLEDQDRSLWDRTLIA
                     DGVGLVERALASRRAGPYTLQAAISAVHAEAAGSGTTDWAQIVALYDLLLRMEPSPVV
                     ELNRAVAVAMRDGAAAGLALVEAILARGELADYRLAHAARADLCRRLGRKEEALSSYR
                     RALELTRQEPERRFLERRLAELKA"
     gene            1944..2141
                     /locus_tag="AC158Q_02850"
     CDS             1944..2141
                     /locus_tag="AC158Q_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338735.1"
                     /translation="MEWIMLGLEVTRRLALKMGPYLAVEILLPGGTLFALLLYWYRRK
                     PGPKQISDHAVDSEPARSTMG"
     gene            2194..2619
                     /locus_tag="AC158Q_02855"
     CDS             2194..2619
                     /locus_tag="AC158Q_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HIR3338736.1"
                     /translation="MRFMVIVKATQDSEAGVLPTEELLTAMGRYNEELASAGIMLAGD
                     GLQPSAKGARVRFSGARRTVVDGPFAETKELVAGFWIWKVKSKEEAIEWVKRCPNPMP
                     GDSEIEIRQLFEAEDFGEQFTPELKERAERVYAQVSGSK"
     gene            2643..>3059
                     /locus_tag="AC158Q_02860"
     CDS             2643..>3059
                     /locus_tag="AC158Q_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3338737.1"
                     /translation="MPKVNPVPDWMHTVTPHLVCAGAADAIEFYKKAFNAVEMARVPG
                     PQEKIMHACIRIGQSPVMLVDEFPEWGAFSPKSLKGSPVTIHLQVEDVDAFVARAVAA
                     GAKITMPVADAFWGDRYGKLEDPFGHHWSVATHIRDM"
BASE COUNT          534 a          960 c         1046 g          519 t
ORIGIN      
        1 cattgcgggc gagctgttca atcatctcgg caagacgaac atcgcggcgg ttcagtacaa
       61 gggcggcggt cccgcgctgg ttgcggcgat cagcggcgag gtgagcatcg cgttctcggg
      121 cgtcgccggc gcggtgggct tcgtgcagtc agggcgcctg cgcgcgctcg gtgtgacgag
      181 ccggaagccg gtggcggcgc tgcccggcgt gccgccgatc gccgaatccg gcctgcccgg
      241 ctacgagttc gtcgcgtggt tcgtgctggc cgcgcccaag ggcaccccac gcgaaatcat
      301 cgtcatgctc aacgagcact tccgcaaggc catgagcttt cccgatacca tccggcgctt
      361 cgaggccgat gggctggacg tcgtcggcag cacgccggaa caggcggccg ccaccatgga
      421 atccgagctg aagaaatggg cggtggtgat caaggaaagg ggcatgcggg cgaattagcc
      481 ccgcggcctc gctgccgatg atcgaggacg gccgcggcgg ggtgcgcgaa acggtggatg
      541 ccgtctaccg gtcggaatcg cgccgcgtct tcgccaccct ggtccgcctc ctcggagatt
      601 tcgatctcgc ggaagaagcg ctgcacgaag ccttccgcgc ggcgctcgag cagtggccgc
      661 gcgacggcgt gcccgcgaat ccgcgcgcgt ggctcgtgtc ggccgggcgc ttcaaggcga
      721 tcgacggtat gcgccggcac gcccggttcg accccctcga cgaggacgcc gtcgaggatc
      781 tcgcggcgcc tgacgatccg gcaacgtggg gagccgagga gatcgaggac gaccgcctgc
      841 ggctcatctt cacctgctgc catccggcat tgccgcccga cgcgcaggtg gcgttgacgc
      901 tgcgcgaggt gtgcggtctc acgacggaag agatcgcgca cgccttcttg gccgcgccgc
      961 ccaccctggc gcagcgcatc gtgcgcgcga agaccaagat ccgcgccgcg aagatcccct
     1021 accaggtgcc ggcgccggcg gacctgccgg agcgcctcga tagcatgctg cgcgtaattt
     1081 atttggtgtt caacgagggc tacgccgcgt cctcgggcgc ctcgctcacg cggcacgatc
     1141 tgtcgggcga agcgatccgg attgccgggc tgctggtcga tttgttgccg gagcccgagg
     1201 cgatgggact gtacgcgctg atgctgctgc acgaatcgcg gcgtggcgcg cgcgccacgc
     1261 ccgcgggaga cctgattctc ctggaagatc aggatcgctc gctctgggac cgcacgctga
     1321 tcgccgatgg cgtgggattg gtggaacggg cgctggcatc gcgccgcgcc ggaccgtata
     1381 ccctgcaggc ggcgatctcg gcggtgcatg ccgaggcggc cggttccggc acgaccgatt
     1441 gggcccagat cgtcgccctg tacgacctgc tgttgcggat ggaaccgtcg cccgtggtcg
     1501 agttgaaccg cgccgttgca gtggcgatgc gcgacggggc tgctgccggg ctcgcgctgg
     1561 tggaagcgat cctcgcgcgc ggcgagctcg ccgattaccg ccttgcccac gcggcgcgcg
     1621 cggacctgtg ccggcggctg ggccgaaaag aggaagctct ctcctcctac cgcagggccc
     1681 tcgaactcac ccgccaggaa ccggagcgcc ggttcctcga gcgccgcctg gccgaactga
     1741 aggcgtaagg tgtaaggcgg agggcggagg gcagaaggtc ctgacggcgc tgacgctgtc
     1801 aatgttcccg cgctgcacgg gacatacttt ctcctttcgc ctttcaccca tcgtcttcag
     1861 accgatgtcg atatcacgct tacccactcg accattcagg gaaggcccgg tgttcgggcc
     1921 tcgctgtcta ccgggagtgg tgaatggaat ggatcatgct tgggctggag gtgacgcgcc
     1981 gcctggcgtt gaaaatgggg ccttatcttg ccgtggagat attgctgccc ggcggaacgc
     2041 tgtttgccct gctgctttat tggtaccggc gcaagccggg gccaaaacaa atttccgatc
     2101 acgctgtcga ttcagaaccc gcccgatcga ccatgggata acgacccaag gatgcactaa
     2161 acggatatca atatatttca aggagatacg acaatgcgct tcatggttat cgtgaaagcc
     2221 acccaggact ccgaggccgg agtcctgcct accgaagagc tgctgaccgc aatgggccgg
     2281 tacaacgagg aactggccag tgcaggcatc atgctcgccg gagacggact gcaaccgagc
     2341 gcgaagggcg cgcgtgtgcg attttccggg gccaggcgga ccgtggtcga cggccccttt
     2401 gccgaaacca aggagctcgt cgccggtttc tggatctgga aagtgaagtc gaaggaagaa
     2461 gcgatcgaat gggtcaagcg ctgccccaac cccatgccgg gggattcgga gatcgagatt
     2521 cgccagctgt tcgaggcgga agacttcggc gagcagttca cgccggagct gaaggaacgg
     2581 gccgagcgcg tgtacgccca ggtctccggc agcaagtaat cccgcaacga aaggaaaaat
     2641 ccatgccgaa agtaaatcct gttccggact ggatgcacac ggtgacgccg cacctggttt
     2701 gcgccggcgc cgccgacgcc atcgagttct acaagaaagc attcaacgcg gtggaaatgg
     2761 cacgggtgcc cgggccgcag gagaaaatca tgcatgcgtg catccggatc ggtcagtcac
     2821 ccgtcatgct ggtcgacgaa tttccagaat ggggcgcgtt cagtccgaag tcgctcaagg
     2881 gctcgcccgt cactatccat ctgcaggtcg aggatgtcga cgcgttcgtc gcgcgcgccg
     2941 tcgcggcagg ggcgaagatc acgatgccgg ttgcggacgc gttctggggc gatcgttacg
     3001 gcaagctcga ggatccgttc ggtcatcatt ggtcggtcgc cacgcacatc cgcgacatg
//
LOCUS       DBNGSG010000111         5925 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3263462, whole genome shotgun sequence.
ACCESSION   DBNGSG010000111 DBNGSG010000000
VERSION     DBNGSG010000111.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5925)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5925)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5925
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3263462"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="AC158Q_02865"
     CDS             complement(<1..758)
                     /locus_tag="AC158Q_02865"
                     /inference="COORDINATES: protein motif:HMM:NF014051.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HIR3338738.1"
                     /translation="MSNDPTAQGREPGAAMPRLRDQAFSGARWTSLASVVRIVLQFAQ
                     LAILARLLPPADFGNMAVVLAVIAFAQIFADLGVSNAIITRTSITASTLSSLYWLNIF
                     ASACVALILLVCAPWVASFYGEPVLQPLLSLAGLHLLVTASWQQLRVLAEKELRFETL
                     ARIEIGASIAGLVVAVTMAWAGAGVYALIGGVLAVSATGAGLAWLLLARGWRPQLRLR
                     FGEIRGYLAFGTYMIGNDIANTVSSQIDVLLGAR"
     gene            complement(755..1930)
                     /locus_tag="AC158Q_02870"
     CDS             complement(755..1930)
                     /locus_tag="AC158Q_02870"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: protein motif:HMM:NF019073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008417 - fucosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase family protein"
                     /protein_id="HIR3338739.1"
                     /translation="MNILHLIHDNKFFSFVTEVFGARPGVQNRYVAHVPDPAAALQHI
                     GGVKLWRRVGNRYFFSRQVREDLEWSDCLIVHYAYAPGALMMLRAPARVTVVWSGWGA
                     DYLDLTAAGGQAMHTGETARLLADIRRKRLGRGLREAGRCLLEAAWLRFVDRPLRVAA
                     IARADLFSAPLREDFDLLRAALGSRLRAQYAQLHYASVERTFRPGPEALVGGDILLGN
                     SATPTNNHAEMLHLLARRDLGDRKVIVPLSYGDAYYRDAIAALGRRLLGDHFEPVLDF
                     VPLSDYNTLIARCSTVIMNHRRQQAIGNIGAMLYRGARVFLDDSGAVYRFLRAQGAHV
                     FSTSLLKETGDAVFDLLKPEQRMQNRAVLDVLWSHDTVMKNTAGFVEAAGQCLRARA"
     gene            complement(2024..3127)
                     /locus_tag="AC158Q_02875"
     CDS             complement(2024..3127)
                     /locus_tag="AC158Q_02875"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR3338740.1"
                     /translation="MKPITVTQPFLPPLKEFLPYLEEIWQSKWLTNGGPFHQQLESEL
                     ARHLGVEHIALFTNGTIALVSALQALRISGEVITTPFSFVATAHSLLWNGIKPVFVDI
                     HPDTLTLDPARIEEAITPQTTAIMPVHVYGRPCDVDAMKKVADTYGLKIIYDAAPAFG
                     VTYRKQSLLKFGDLSILSFHATKVFTTFEGGAIVCPDANMKQRIDYLKNFGFADEVTV
                     VAPGINGKMNEVQAALGLLQLKYIDASIASRCAIDARYRQELRGVRGIRVPSLPAESK
                     QNYSYFPVLVGAEYPISRDALYDRLRQNGIHARRYFYPLISEFPMYRGLPSAAPGRLP
                     VASAIARQILCLPIYPALTDEVVTRVAQVIREA"
     gene            3230..3955
                     /locus_tag="AC158Q_02880"
     CDS             3230..3955
                     /locus_tag="AC158Q_02880"
                     /inference="COORDINATES: protein motif:HMM:NF019846.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR3338741.1"
                     /translation="MRHPERWTPTKFVNRRGRWQGSRDTRELALASRLAGDLVAAAYA
                     KHLPAHAAGRLLDLGCGKAPLHGMVRPHVRDSICADWPGSHHGSEHVDVFLDLNRPLP
                     FRDGCFRTVLLSDVLEHIYQPWPLWLEINRVLEAGGKLIANMPFIYWIHETPHDYYRF
                     TEHALARMADDAGFSVIVLEPLGGPAAVAADLLAKQLAPLRWIGRYLAQAVQSIAPRR
                     AGRGAVMTLGYFVVAEKAGAARA"
     gene            3952..4830
                     /locus_tag="AC158Q_02885"
     CDS             3952..4830
                     /locus_tag="AC158Q_02885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3338742.1"
                     /translation="MTPGPAITVYLPTRNRPALLKRAVASVLGQSYPHFEIVIALDRP
                     SEETRSITGELSGAAAHARRVLRFVESDKPGACAARNCALAAAAGELATGLDDDDYLE
                     RDHLATLAGSFDPDRHAFVFTGWRRIHPGKTALRVEIPVDIPAGRLDLDSLLRKNVVG
                     NQVLTLTSRLRDAGGFDESLPAWHDYDLWLRLVKRYGPGWGLPGHSYVVDEVSADERI
                     SGSADRIDGAYRRFLEKHVEYAEPTLQAFLRLTRAAYGLGGLSLADVARVVVSSGTTR
                     ASIDAVYYYLYGLRYA"
     gene            4912..>5925
                     /locus_tag="AC158Q_02890"
     CDS             4912..>5925
                     /locus_tag="AC158Q_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338743.1"
                     /translation="MAGVSLAFSAIIALALTLLRGDVASPFEIPVYRHLILFQDYYLI
                     IPFMAVLMAALVEPVRAAGARLAHWSGSNVWLVAIATTALLGYGSHAAYHAHPLSLDE
                     YAVVFQSRVFAEGKLSGLFPPPLVDWLVPKFFQGRFLRLDMESGAVAAVYWPGFALLL
                     APFSAFGVPWLLNPILGGATVLVMHRLGLALFGNAERAGFVVLLTLASPAITINALSY
                     YSMPAHLLANALFVQLLLPASAGRAFVAGLVGSIALVLHNPVPHMAFALPWIVWLAFQ
                     PSRWKLLGALAAGYLPASLVLGWGWAIFLHNLGSPASLADLATPGGAVSTFQNRLGSV
                     LGWT"
BASE COUNT         1019 a         1953 c         1886 g         1067 t
ORIGIN      
        1 attctggcgc ccagcaacac gtcgatttgc gagctcacgg tattggcgat atcgtttccg
       61 atcatgtagg tgccgaaggc gaggtatccc cgtatttcac cgaatcgaag ccggagctgc
      121 ggtcgccagc cacgcgcaag aagcagccac gccagtccgg cgcccgtggc ggaaactgcc
      181 agcacacctc cgatgagcgc atagacaccg gctcccgccc atgccatcgt gaccgcgacc
      241 accagcccgg cgatgctggc gccgatctcg atcctggcca gcgtctcgaa gcgcaattcc
      301 ttttccgcca acacgcgcag ctgctgccag gacgcggtaa cgagaaggtg caagccagcg
      361 agcgacagca ggggctgcag caccggttca ccatagaacg acgccaccca cggcgcgcaa
      421 accagcagga tgagcgccac gcaggcgctc gcgaaaatgt tcagccagta gaggcttgaa
      481 agcgtcgacg ccgtgatgct ggtgcgggtg atgatggcat tacttactcc caggtccgcg
      541 aagatttgcg cgaacgcgat cacggcaagc accacggcca tgttgccgaa gtcggccggc
      601 ggcagcagcc gcgcgagtat cgcgagctgc gcgaactgca gaacgatacg cacaaccgaa
      661 gcgagcgaag tccagcgcgc accggaaaag gcctggtccc tgagtcgcgg catggcggcg
      721 cccggttccc ggccctgcgc cgtcgggtcg ttgctcacgc ccgcgccctc aggcactgcc
      781 cggccgcttc aacgaatcct gccgtgtttt tcatcacggt gtcgtgcgac cacaggacat
      841 ccagcaccgc ccggttctgc atgcgctgct caggcttcag cagatcgaaa acggcgtcgc
      901 cggtctcctt cagcaacgag gtgctgaaca cgtgagcgcc ctgtgctctg agaaaccggt
      961 agaccgcgcc actgtcgtcg agaaaaaccc tggcaccccg gtacaacatc gcaccgatgt
     1021 tgccgatggc ctgctggcgg cggtgattca tgatgacggt cgagcaacgc gcgatcaggg
     1081 tgttgtagtc cgagaggggg acgaaatcga gaaccggttc gaaatggtcc cccagcagcc
     1141 gccggccgag cgcagcgatg gcgtcacggt aataggcatc gccgtaactc aacggcacga
     1201 tgaccttgcg gtcccctagg tcgcgccgcg caagcagatg aagcatttcg gcatgattgt
     1261 tggtgggcgt ggccgagttg cccagcagga tgtcgccccc gaccagggct tccggcccgg
     1321 gacggaacgt ccgctcgacg ctggcgtagt gcaattgggc atactgtgcc cgcagcctcg
     1381 acccaagcgc cgcgcgcaac aggtcgaagt cttcccgcag cggcgccgag aacaggtcgg
     1441 cccgcgcgat ggcagcaacg cgcagcggtc ggtcgacgaa gcgcagccag gccgcctcca
     1501 acaagcatct ccccgcctcc cgcaagccgc gcccgagccg cttgcgccgg atgtctgcga
     1561 gtagccgggc cgtttccccg gtgtgcattg cctgcccgcc cgcggctgtg aggtcaagat
     1621 agtcggcgcc ccagccgctc cacaccacgg tcacgcgcgc cggggccctc agcatcatga
     1681 gcgcgcccgg cgcgtacgcg tagtgaacga tcaggcaatc ggaccattcc aggtcctcgc
     1741 gcacctgccg cgagaagaaa tagcggttac cgacgcggcg ccaaagcttc acgccgccga
     1801 tatgctggag cgccgctgca ggatccggaa catgcgcgac gtaccggttc tgaacgccag
     1861 gccgcgcgcc aaacacctcg gtcacgaagc tgaaaaattt gttgtcatga atgaggtgca
     1921 ggatgttcat gatctccggc cgcaaccccc gggcaggcgt tgctcgagtc ccggcacgtt
     1981 ccatccccga ctgcgcaact cggtttccgg gtaatgcgac acgtcaggcc tcccggatga
     2041 cctgggccac gcgcgtcacc acttcgtcgg tgagcgcggg gtagatgggc aggcatagta
     2101 tttggcgagc aatggcgctc gccaccggca gcctgcccgg cgccgccgac ggcaagcccc
     2161 gatacatcgg gaactcgctg ataagcggat agaaatagcg gcgcgcgtgg atcccgttct
     2221 ggcgcaggcg gtcgtagagc gcgtcgcggc tgatcgggta ttccgccccg accagaaccg
     2281 ggaaatagga gtaattctgc ttgctctcgg cgggcaacga tgggacccgg atgccgcgca
     2341 cgccgcgcag ctcctgtcgg tatctagcgt cgatagcgca acggctggca atcgaggcat
     2401 cgatgtattt cagctgcagc agcccgagag ccgcctgcac ctcgttcatc ttgccgttga
     2461 tgccgggcgc tacgacggtc acctcgtcgg cgaatccgaa gttcttgaga taatcgattc
     2521 gctgtttcat gttcgcgtcc gggcagacga tggcgccgcc ctcgaaggtc gtgaagacct
     2581 tcgtggcgtg aaagctgagg atggaaaggt cgccgaactt gagcaggctc tgcttgcggt
     2641 aggtcactcc gaacgccggc gccgcatcgt agatgatctt cagcccgtag gtatcggcga
     2701 ccttcttcat tgcgtcgaca tcgcaggggc ggccgtagac gtgcaccggc atgatcgcgg
     2761 tggtctgcgg cgtgatcgcc tcttcgatgc gcgccggatc gagcgtgagc gtgtccggat
     2821 ggatatccac gaagaccggt ttgatgccgt tccacagcag cgaatgcgcg gtcgccacga
     2881 acgagaaggg cgtggtaatg acttcgcccg agatccgcag ggcctgcaac gcgctcacca
     2941 gggcgatcgt gccgttggtg aaaagagcga tgtgctcgac ccccagatgg cgggcgagtt
     3001 ctgactcgag ctgttggtga aaaggaccgc cgttggtaag ccatttgctc tgccagattt
     3061 cctccagata cggcagaaac tccttcagcg gcggcaggaa cggctgggta acggtaatgg
     3121 gcttcatggc tataatggag gtggcgacca gcgcccctgt gtctcgagac ctcttatggt
     3181 accttgggga ggtctggatc agtgagcaat aaatgccggt tgatcaacga tgcgtcatcc
     3241 cgaacgctgg acgccgacca agttcgtgaa ccgccgcggc cgctggcagg gcagccggga
     3301 cacgcgcgaa ctcgctcttg cttcccgcct ggcgggcgac ctggtcgcgg cggcctatgc
     3361 gaaacacctg cccgcccacg ccgcgggacg tctactcgac ctgggttgcg gcaaggcccc
     3421 cctgcacggc atggtacggc cccacgtacg ggacagcatc tgcgccgact ggcccggctc
     3481 gcaccatggc agcgagcatg tcgacgtctt tctcgatctc aaccgaccgc tgcccttccg
     3541 tgacggttgc tttcgcaccg tgctactgtc ggatgtcctt gagcacatct accagccttg
     3601 gccgctctgg ctcgaaatta accgcgtcct cgaagccggc ggcaagctga ttgcaaacat
     3661 gcccttcatc tactggatcc acgaaactcc gcacgactat taccgcttca ccgagcatgc
     3721 gctggccaga atggcggacg acgccggatt cagcgtgatt gtgctcgagc ccctgggcgg
     3781 gcccgccgcc gtggccgcgg atctgctggc gaagcagctt gcaccgctgc gctggatcgg
     3841 tcgctacctg gcgcaagctg tgcagtcgat agcgccacgg cgggccgggc gcggcgccgt
     3901 gatgacgctc ggatacttcg tcgtggccga gaaagccggt gctgcccgcg catgaccccc
     3961 ggtcccgcca ttacggtcta ccttcccacc cgcaaccgcc cggcgctgct gaagcgcgca
     4021 gttgcctcgg tactcggaca gagttacccc catttcgaaa tcgtgatcgc attggaccgc
     4081 ccatcggagg aaacccgatc gataacaggc gaactgtccg gggccgcagc gcatgccaga
     4141 agggttctgc gcttcgtcga gtccgacaag ccgggcgcct gcgcggcgcg caactgcgcg
     4201 cttgcagcag cggccggcga attagccacg ggactggacg acgacgacta cctggagcgc
     4261 gaccacctgg caaccctcgc cggttcgttt gaccccgacc ggcacgcctt cgtcttcacc
     4321 ggttggcgcc ggattcatcc tggcaagact gcgctccgcg tcgaaatacc tgtggacatt
     4381 cccgcaggcc gattggacct cgactcgctg ctgcgcaaga acgtggtggg caaccaggtc
     4441 ctgacgctga cttcacggct gcgcgatgcc ggcggattcg acgaatcgct gcctgcctgg
     4501 cacgactacg acctgtggct gcgcctcgtc aaacgatacg gtccgggatg ggggctgccc
     4561 ggtcacagtt acgtggtcga cgaagtatcg gcggatgagc gtatctccgg aagtgcagac
     4621 cgaatcgacg gagcctaccg ccgatttctc gagaagcatg tggaatacgc cgagccgacg
     4681 ctgcaagcct tcctgcggtt gacccgggcc gcctacgggt tggggggatt aagtctggcg
     4741 gatgtggcgc gggtcgtcgt ctcgtccggt actacacggg caagtatcga tgcggtctac
     4801 tactacttgt acgggctgcg ctatgcctag tgcgaccttg gctgcatccc atcctgtccc
     4861 cggggtacgg ccgtgacggc gttgccacct ggtggagggc agaccctccg ggtggcgggc
     4921 gtcagccttg ccttctctgc catcattgcg ctcgcgctca cactgctgag gggggacgtc
     4981 gcgagcccct tcgaaatccc ggtttaccgc cacctcatcc tgttccagga ctactacctg
     5041 atcatcccat tcatggcggt gctgatggcc gcgttggtag agccggtgcg cgccgccggc
     5101 gcgcgcctgg cccactggag tggctcgaat gtctggctgg tggcgatcgc cacgacggcg
     5161 ttgctgggct acggcagcca tgccgcgtac catgcgcacc cgctttccct cgacgaatac
     5221 gcggtcgtgt tccagagccg cgtgttcgcg gagggtaagc tgagcgggtt gttcccgccg
     5281 ccgctcgtcg actggctggt tccgaagttc ttccagggcc gctttctccg actcgacatg
     5341 gaaagcggcg ccgtcgcggc ggtgtactgg cccggcttcg cgctgctcct cgcgccgttt
     5401 tccgcgttcg gtgtgccctg gctgctgaat cccatactcg gcggcgccac ggtattggtc
     5461 atgcatcgcc tggggctggc gctttttggc aacgcggagc gcgcgggatt cgtggtgctg
     5521 ctcaccctgg cttccccggc catcacgatc aacgcactgt cctactattc aatgccggcg
     5581 caccttcttg ccaatgcgct gttcgtgcag ctgctgctcc ctgcgtccgc gggccgggcg
     5641 tttgtggcag gcctcgttgg atccatcgcg ctggtgctgc acaacccggt gccgcacatg
     5701 gcgttcgccc tgccgtggat agtctggctc gccttccagc cctcccgctg gaagctgctc
     5761 ggcgccctgg cggccggcta tctgcccgcg tcgctggtgc tgggatgggg gtgggccatc
     5821 tttctgcaca acctcggttc cccggcgagc ctggccgacc tcgccacgcc cggcggcgcg
     5881 gtttcgactt tccagaaccg gctgggcagc gtgctcggct ggacc
//
LOCUS       DBNGSG010000112         2182 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_207686, whole genome shotgun sequence.
ACCESSION   DBNGSG010000112 DBNGSG010000000
VERSION     DBNGSG010000112.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_207686"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="AC158Q_02895"
     CDS             <1..308
                     /locus_tag="AC158Q_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338744.1"
                     /translation="LHTVGGRIGAHNIHENEPEPGAAKLENTVASTWFSAGLRVYDIA
                     DPYRPEEIAAFLPETPKGQRGCRISDVFVDDRSIIYAADRACGGLYVLEYIGKRPLN"
     gene            complement(634..1035)
                     /locus_tag="AC158Q_02900"
     CDS             complement(634..1035)
                     /locus_tag="AC158Q_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338745.1"
                     /translation="MLELDVQLKFGELLFALVAAGVIVVGAIFVAVNVVRRRTRPPLY
                     VEAGGVVLSVGEEKLLELVARSAGKDQNLAALARALNADEIAVYSIARELKRKALVVE
                     IADLSGASPRFELSASGNEAALARNYLKFTI"
     gene            complement(1217..1588)
                     /locus_tag="AC158Q_02905"
     CDS             complement(1217..1588)
                     /locus_tag="AC158Q_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338746.1"
                     /translation="MGDPIGTSKPAAGAAELLSIPRYLEELRRVARYRPGPPIGEPLD
                     AAVKKIEKNPAFAQSRLLTRILAALTYQEGEFRRSEVAALDSDTLAMVIMLMDRQAAG
                     TTTREEWVCAVELARAAELAA"
     gene            complement(1595..1864)
                     /locus_tag="AC158Q_02910"
     CDS             complement(1595..1864)
                     /locus_tag="AC158Q_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HIR3338747.1"
                     /translation="MNIMIWLLAGGIAGWIGYTYMQFNEDRGMIVSIIIGMLGGFFGG
                     KVLAPMLGAVAASPADFSLPGVVVALASAAACLALGNLISNRYGI"
     gene            complement(1906..>2182)
                     /locus_tag="AC158Q_02915"
     CDS             complement(1906..>2182)
                     /locus_tag="AC158Q_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DesA/ISL3 alpha bundle tail domain-containing
                     protein"
                     /protein_id="HIR3338748.1"
                     /translation="PDLQTLLAVITHRYDVQAKYAKTLKRIGTGARDAHALQTMHSMR
                     RELVTLWERSPLSKEHLVLRLQDWCLRAEASGIHPLVEFSQKLRCYA"
BASE COUNT          389 a          738 c          670 g          385 t
ORIGIN      
        1 atctccacac ggtcggcggc cgcatcggcg cgcacaacat ccacgagaac gagcccgagc
       61 cgggcgccgc gaagctggag aacacggtgg cctcgacctg gttcagcgcc ggcctgcgcg
      121 tctacgatat cgccgacccg taccgcccgg aggagatcgc cgcgttcctg ccggagacac
      181 ccaagggcca gcgcggctgc cgcatcagcg acgtgttcgt ggacgaccga tccatcatct
      241 acgccgccga ccgcgcgtgc ggcggcctct acgtgctgga atacatcgga aaacgcccgc
      301 tcaactaggc cagcgattta gccgccgatg cgcgccgata agggcgggag aacagcacat
      361 caacgccgcc ggcctttcat ggtctccgtc tcggcctgct gctgacggac attcacgcct
      421 gaccggcccc tgctacgcgg cccggaacgg gcaaggatac agcggggcaa agcccgcttc
      481 gggtcgatcg cagtcgattt cggccacgcc accagcccag gtgaaatatg gacttatctg
      541 tcccgcccgc gtgttgagcg gcaacgtgtc gtccgttcga acacgcggag ttaaaccggc
      601 gcgcagcgtc cggatataac ggcatccggc ggttcaaatc gtgaatttga ggtagttgcg
      661 cgccagcgct gcctcgttgc cgctcgcgga cagctcgaag cgggggctgg cgccggaaag
      721 atcggcgatt tcgacgacca gggctttgcg tttcagctct cgcgcgatgg agtagacggc
      781 aatctcgtcg gcattcagcg cccgcgccag ggcggcaagg ttctggtcct tgccggcgct
      841 gcgcgcgacc agctcgagga gtttttcctc gcccaccgac aaaaccacgc cgcccgcctc
      901 gacgtagagc ggcggccggg tgcgccggcg gacgacgttc acggccacga aaatcgcgcc
      961 gacgacgatg acgcctgccg caaccagcgc gaacagcagc tccccgaatt tcagttgaac
     1021 gtccagttcc agcatggcga gctccccaac ccgtcacgac gacgcgccgc gccagcagcg
     1081 caagcttgtt tttcgatttg ttattgcacc gccattctag acccaatcgt actccgcggt
     1141 tcaattattg ttattttttt cctggcggtc tcggcggttc aattattgtt ttccttcgtg
     1201 tcctggctct taatcactat gcggccagtt ccgcggcccg cgccagctcg acggcgcata
     1261 cccactcctc gcgcgtcgtc gtcccggcgg cctgccggtc catcagcatg atgaccatgg
     1321 cgagcgtgtc cgagtcgagg gctgcgactt ccgagcgccg gaattcgccc tcctggtagg
     1381 tcagcgctgc cagtatgcgg gtcagcaggc gggactgcgc gaacgcgggg ttcttctcga
     1441 tcttcttcac cgcggcatcc agcggctccc cgatcggggg gccgggacga tagcgcgcga
     1501 cacgccgcaa ctcttccagg tagcggggta tcgaaagcag ctcggccgcc ccggccgccg
     1561 gtttcgatgt gccgatggga tctcccacgc cggctcaaat gccgtagcgg ttggaaatca
     1621 ggttgccgag ggcgaggcaa gccgccgcgc tcgcgagcgc gaccaccacc ccgggcaggc
     1681 tgaagtcggc gggactcgcc gcgacggcgc ccagcatcgg cgcgagcact ttcccgccga
     1741 agaacccccc gagcatgccg atgatgatcg agacgatcat gccgcgatct tcgttgaact
     1801 gcatgtacgt atagccgatc caaccggcaa tgccccccgc cagcagccaa atcatgatgt
     1861 tcatttgctt cctcccggtg taatcaacat gaaaaccgtg cggccctagg catagcaccg
     1921 cagcttttgc gaaaactcga ccagggggtg tatgccactc gcctcggcgc gcaggcacca
     1981 gtcctgcaac cggagcacca ggtgctcctt ggataatggg gagcgctccc acagcgtcac
     2041 gagctcgcgg cgcatggaat gcatggtctg aagcgcatgc gcgtcccgcg cgccggttcc
     2101 gatccgcttc agcgttttgg cgtactttgc ctggacatcg tagcgatggg taatcaccgc
     2161 cagcagcgtc tgcaggtcgg gg
//
LOCUS       DBNGSG010000113         3941 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1566356, whole genome shotgun sequence.
ACCESSION   DBNGSG010000113 DBNGSG010000000
VERSION     DBNGSG010000113.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1566356"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="AC158Q_02920"
     CDS             complement(<1..877)
                     /locus_tag="AC158Q_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nudix family hydrolase"
                     /protein_id="HIR3338749.1"
                     /translation="MLGQRPAGKVYAGYWEFPGGKLEQGEAPLAALTRELREELGIEV
                     ERAYPWLTRDYDYAHAAVRLRFFRVVRWSGTLHGRENQRFAWQLPDAISVDPLLPANA
                     PILRALRLPPVYAISNAGELGEREFLARLERALAQGLKFLQVREKEMSGEGLLRFAAE
                     VVRLAHAHGAQVLVNGDAGLAQRAGADGVHLTAARMKRLDRRPVSGLVGASCHDAGEL
                     ARAQALGADFVVLGPVQPTPSHPGATGIGWEPFAGLLKDSRVPVYALGGLKAADLERA
                     WESGAHGISMMRGAWS"
     gene            complement(1009..1893)
                     /locus_tag="AC158Q_02925"
     CDS             complement(1009..1893)
                     /locus_tag="AC158Q_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3338750.1"
                     /translation="MNDAAKLIKQAEGILARLERLLPQEPPPTDWKASTAFRWRKKNG
                     RGHIEPVRHVHRVALRDLQGIDTQKELVEGNTRRFVEGHPANNVLLTGARGTGKSSLI
                     KALLNKYASKGLRLIEVEKQDLVDLPDIVDHIADRPERFVLYCDDLSFEADEPGYKAL
                     KVVLDGSIAAATENCLIYATSNRRHLMPEFMQENLDYKHVGEEIHPSETSEEKVSLSE
                     RFGLWTTFYPFDQDDYLRIVAVWLEHFKAPGARSEAAQRAALQWSLQRGSRSGRVAWQ
                     FARDWAGKARMKAEGGKS"
     gene            complement(1941..3179)
                     /gene="argJ"
                     /locus_tag="AC158Q_02930"
     CDS             complement(1941..3179)
                     /gene="argJ"
                     /locus_tag="AC158Q_02930"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017333474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - L-glutamate N-acetyltransferase
                     activity, acting on acetyl-L-ornithine as donor [Evidence
                     IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HIR3338751.1"
                     /translation="MPVNLKPPDPRSLLPVKGVSLGVTEAGIRKPGRKDLLVMRLEPG
                     ARVAGVFTQNRFCAAPVVVARQHLTMLDPDASIRALVVNTGNANAGTGKEGIAAARQT
                     CNELAKRLGCSSAQVLPFSTGVIMEPLPVDKIAAGLPACLADAREDNWFAAAQAIMTT
                     DTLPKAVSRRVRLGAAEVTVTGIAKGAGMIHPHMATLLGFVATDAKLALPLARAMAAQ
                     AANRSFNCITVDGDTSTNDAFMLVATGAAGTPELSDGKSAEYAALADGVAAVAAELAQ
                     AIIRDGEGATKFITVRVEGGRKEEECRKVAYAIAHSPLVKTAFFASDPNLGRILAAVG
                     YAGIADLAVDMVDLYLDDVLVAKNGGRSPGYHEADGQRVMKQPEITVRVVLNRGAAAA
                     TVWTCDLSHDYVTINAEYRT"
     gene            complement(3286..>3941)
                     /locus_tag="AC158Q_02935"
     CDS             complement(3286..>3941)
                     /locus_tag="AC158Q_02935"
                     /inference="COORDINATES: protein motif:HMM:NF019155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HIR3338752.1"
                     /translation="PDSLEEQWDPLALAKTLEAELRLTAPVAAWLKDEPELERREMIA
                     RAVEAARNLYREKTASVDPQAMRQYERAIMLQSLDTHWREHLSALDHLRQGIHLRGYA
                     QKNPQQEYKREAFELFTMMLESIKAEVTKVLMTVQIRSAEELAAAEELEAPRNVQYQH
                     AEYAGGGDATVEPEAEGDIAVAERPQPFVRPGQKVGRNDPCPCGSGKKYKQCHGRLS"
BASE COUNT          593 a         1374 c         1247 g          727 t
ORIGIN      
        1 agctccaggc cccacgcatc atgctgatgc cgtgtgcgcc ggattcccag gctcgctcca
       61 ggtctgcggc cttgagcccg cccagcgcat agaccggcac ccggctgtcc ttcaacagcc
      121 cggcgaatgg ctcccagccg attccggttg ctccgggatg gctgggcgtc ggctgcacag
      181 gccctagtac gacgaaatcc gctcccagcg cctgcgcgcg cgcaagctcg ccggcgtcat
      241 gacaggaggc gcccacgagg cctgacacgg gtcgccggtc gagtcgcttc atccgggccg
      301 cggtaagatg aacgccgtcg gcaccggcgc gctgcgccag cccggcgtcg ccgttcacca
      361 gcacctgcgc gccgtgggca tgggcaagcc gcacgacctc ggccgcgaaa cgaagcaggc
      421 cttccccgga catttccttc tcgcgcacct gcaggaactt gagcccttgc gcgagggcgc
      481 gctccagccg cgccaggaat tcccgctcgc ccagctcgcc ggcgttactg atggcgtaga
      541 ccggaggcaa ccgcagcgcc cgcaggatcg gggcattggc gggaagcagc gggtcgaccg
      601 agatcgcatc gggcagctgc caggcaaagc gctggttctc gcgcccgtgc agcgtgcccg
      661 accaccggac cacccggaag aaacgcagcc gcacggcggc atgcgcgtag tcgtagtcac
      721 gggtcagcca ggggtaagcg cgctcgacct cgatgccgag ttcctcgcgt aactcgcgcg
      781 tgagcgccgc gagcggcgcc tctccctgct ccaatttgcc gccgggaaat tcccagtagc
      841 cggcatagac cttgcccgcg ggccgctgac ccagcaagaa ccggccgtcc ggccgcgcga
      901 tgaccgctgc aacgacttca acgcgattcg agggctgagg gctgagggtt gagggctgag
      961 gggcgattgt tacttccgac tttcgacttc cggctttcga cttcatccct aggactttcc
     1021 gccttccgcc ttcattcttg ctttccccgc ccagtcgcgg gcgaactgcc acgccacgcg
     1081 gccgctgcgc gagccgcgct gcagggacca ttgcagcgcc gcgcgctgcg ccgcctcgga
     1141 acgggcgccg ggcgccttga aatgctccag ccacacggcg acgatccgca gatagtcgtc
     1201 ctggtcgaag gggtagaacg tggtccacag cccgaagcgc tcggacaacg acaccttctc
     1261 ctccgaggtc tcgctcggat gtatttcctc gccgacgtgc ttgtagtcga ggttctcctg
     1321 catgaactcg ggcatcaggt ggcgccggtt ggaggtggca tagatcaggc aattctcggt
     1381 tgcggccgcg atcgagccgt cgagcaccac cttcaatgcc ttgtagccgg gttcgtcggc
     1441 ctcgaatgaa aggtcgtcgc aatacagcac gaagcgctcg gggcggtcgg cgatgtggtc
     1501 cacgatgtcc ggcagatcca ccaggtcctg cttttccact tcgatcagcc gcaggccctt
     1561 cgaggcgtac ttgttgagca gcgccttgat gagcgaggac ttgccggtgc cgcgcgcgcc
     1621 ggtcagcagt acgttgttgg cggggtgccc ttcgacgaac cgccgcgtgt tcccctcgac
     1681 cagctctttt tgagtgtcta tgccctggag gtcgcgcaat gcgacccgat gcacgtgccg
     1741 caccggctcg atgtggcccc tgccgttctt cttgcgccag cgaaaagcgg tcgaggcctt
     1801 ccagtccgtg ggcggcggct cctgcggcag cagacgttcc agccgcgcaa gtatcccttc
     1861 ggcctgcttg atcaatttgg cagcgtcatt catcgactta ttgtatttcc tggcggcctt
     1921 ggcgtcttgg cagtgaatgc ttacgttctg tattccgcgt tgatcgtgac gtagtcgtgc
     1981 gacaggtcgc aagtccagac cgtcgcggcc gcggcgccgc ggttcaacac cacccgcacc
     2041 gtgatttccg gctgcttcat cactctttgc ccgtccgctt catggtagcc gggactgcgg
     2101 ccgccgttct tcgccaccag cacatcgtcg aggtagagat ccaccatgtc caccgcgaga
     2161 tcagcgatgc cggcatagcc gaccgccgcg agtatccgtc cgaggttggg gtcggaggcg
     2221 aagaacgcgg tcttcaccag cggcgagtgc gcaatggcgt aggccacttt ccggcattcc
     2281 tcttctttgc gtccgccctc gacgcgcacg gtgatgaatt tcgtggcgcc ctcgccgtcg
     2341 cggatgatgg cttgcgcgag ttcggccgcc acggcggcaa cgccgtccgc caacgccgca
     2401 tattcggcgc tttttccgtc actcaattcc ggcgtgcccg cggccccggt cgccaccagc
     2461 atgaaggcgt cattggtcga ggtgtccccg tccaccgtaa tgcagttaaa tgatcggttc
     2521 gccgcctgcg cggccatcgc gcgcgcaagc ggcagcgcca gtttcgcgtc ggtggcgacg
     2581 aatccgagca gggtggccat gtggggatgg atcatgccgg cgcccttggc gatgccggtg
     2641 acggtaacct ccgcggcccc gagcctgaca cggcgggaaa cggccttcgg cagcgtgtcc
     2701 gtcgtcatga tcgcctgcgc cgcggcgaac cagttgtcct cgcgggcatc ggccaggcac
     2761 gcgggcaggc cagcggcgat tttgtccacc ggcagcggtt ccatgatgac gccggtcgag
     2821 aacggcagca cctgcgccga ggagcagccc aggcgcttcg ccagctcatt gcaggtctgg
     2881 cgcgccgccg cgatcccttc cttgccggtt cccgcgttgg cgttgccggt gttcaccacc
     2941 agggcccgta tcgacgcatc gggatcgagc atcgtgagat gctgccgcgc caccaccacc
     3001 ggcgccgcgc agaagcggtt ctgcgtgaac acgcccgcca cgcgcgcccc aggctcaagc
     3061 ctcatcacca gcagatcttt gcggccgggc ttgcggatgc cggcttccgt tacgcccagg
     3121 ctcacgccct tgaccggcag caggctcctc ggatcgggcg gcttcaggtt aaccggcatg
     3181 cgcttgagac atcagcaaac gatgacgccg ggattatagc ggggaaaacc cgccgggccg
     3241 taaacgcagg accggtcact cgtcaccggt cactcgtcac tccctctagc tgagcctgcc
     3301 atgacactgc ttgtacttct ttcccgaacc gcacgggcac gggtcattgc gcccgacctt
     3361 ctggccgggg cggacgaacg gctgcggccg ttcggccacg gcgatatcgc cttccgcctc
     3421 cggctcgacc gtcgcgtcgc cgccgcccgc gtattccgcg tgctggtact ggacattcct
     3481 cggcgcctcc agctcttcgg ccgcagcgag ttcctccgcg ctccggatct gcaccgtcat
     3541 caggaccttg gtgacctcgg ccttgatcga ctcgagcatc atggtgaaca actcgaacgc
     3601 ctcgcgcttg tactcctgtt gcgggttctt ctgggcgtag ccacgcaggt ggatgccctg
     3661 gcgcaggtga tcgagcgcgg acaggtgctc gcgccagtgc gtatccaggc tttgcagcat
     3721 gattgcgcgc tcgtactggc gcatcgcctg cggatcgacg ctggcggttt tctcccggta
     3781 cagattgcgc gcggcctcga ccgcgcgcgc gatcatttcc cgccgctcca gttccggctc
     3841 gtccttgagc caggccgcga ccggggccgt caggcgcagc tcggcttcca gcgtttttgc
     3901 cagggccagc gggtcccact gctcttccag gctgtccggc g
//
LOCUS       DBNGSG010000114         3850 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2415656, whole genome shotgun sequence.
ACCESSION   DBNGSG010000114 DBNGSG010000000
VERSION     DBNGSG010000114.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2415656"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="AC158Q_02940"
     CDS             complement(<1..768)
                     /locus_tag="AC158Q_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338753.1"
                     /translation="MPVLTYLAIPGLLPILAPLPGLALLPALALLSTLAVLAALALLA
                     ALSLLILLKLARQFFRLPPQLALLTRKPLELAFHLLLRKGVALLRKLLLAAAQPILPS
                     RKITNLVERLISRLLLLFRLAARWSLVIGFLLASQLAVEERRQILLPPIVACAACSIR
                     LLLDLAATNLRLGAQQLVERGHLAREGVAGLQCIELLIRTDHRGDGSADRLHHRVTRL
                     SGRGAAPAGQAAPPVARALHFRMHGVRARVEIALPARN"
     gene            complement(799..1533)
                     /locus_tag="AC158Q_02945"
     CDS             complement(799..1533)
                     /locus_tag="AC158Q_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338754.1"
                     /translation="MTATLIASLTPRLTALTAGFTRRPIAKVRKLAIERRQLFAKTAY
                     TRQLLAQLLRCFTVATGGAELIRQTVESTRNRLTFALRRCLPAALRIAGLAFRTGGVT
                     HPLRQPLSVEILRSFVQRIPLSLVSFPRAAGHLSHRFFETLRPVGKTLLFRRPLPGSI
                     AAITGTARSGCRIASEAPLSIGKLPRFQLQLTEGTTPIVGLRSAHLLFDVAQPFSRPA
                     AAGARLPQVVAPKLACSALHVLGRLL"
     gene            1579..1911
                     /locus_tag="AC158Q_02950"
     CDS             1579..1911
                     /locus_tag="AC158Q_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338755.1"
                     /translation="MEGPWQEARELLNELRQNEEVVPADADGFNPGRSAPGTEAWKQD
                     FEKWDELKVQLAAALERVERTTADRLRGQQSRDRLNAGATQAVPESYRRLVEKYYRAI
                     ASGEGRGR"
     gene            1953..>3850
                     /locus_tag="AC158Q_02955"
     CDS             1953..>3850
                     /locus_tag="AC158Q_02955"
                     /inference="COORDINATES: protein motif:HMM:NF018757.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HIR3338756.1"
                     /translation="MQLAVSFPWWMLLGLAVATAAVAWASYAGAIVALSPRHRAALVS
                     LRALTLALLVLCLLRPVRVIPPDTTSDAVVPILIDASRSMALADVDGSRRLDIAQKLV
                     QSEIQPALERRFRQELWSFGDGLEQIAERGLVADARRSDLSGALRAVRERYRERQVAG
                     IVVISDGGDTGSQDAAAAVEEGGAPVYTIGVGAPRVTPDLEVLDLSAGDAAVADSSID
                     ISVAAVSRGGTAPFDLRVLANGRPVDLRRVTPAADGSPVRAVFTVTPARETATLYTVE
                     IPSGAGERVLENNRRSVLVEPPGRVRRILMIEGTPGFEHSFIKRALSTDPGFEIDSVV
                     RKGRDARGAATYFVLATPTRAPRLASGFPQQRAALYEYDAIVLANVEPDTLSRAQLQL
                     LADFVEQRGGGLLVLGGKSFAQQGFAGTPLEEALPLRLTDRGNGVVRAANRPESRLTV
                     RLTADGLTHPVMRTDGTAEGLDRRWQAMPALAGVASLGALRPGAQALAVVNAADGPRP
                     LVAVQRYGQGRVVIFAGEASWRWRMQMPSTDRTHELFWRQAVRWMSSPAPDRVSIGTV
                     PSLVPGSTAAISVAVRTEEFASVGDASVRLRVTRPDGSAQDVAAALADSQTGSYLGEV
                     PLEEPGVYR"
BASE COUNT          666 a         1176 c         1429 g          579 t
ORIGIN      
        1 gttgcgcgcg ggcagcgcga tctccacgcg cgcgcgaacg ccatgcatac ggaaatgcag
       61 cgcgcgagcg accggcggcg ccgcctgccc ggcgggcgcg gcgccccgcc cgctcagacg
      121 ggtgacgcga tggtggaggc ggtcggccga gccgtctccg cgatggtcgg tgcgtatcag
      181 cagctcgatg cactgaagac cagcgacgcc ctcccgcgcg agatgaccgc gctcaacgag
      241 ctgctgcgcg cccaggcgga ggttcgtcgc cgcgagatcc agcaacaggc gaatggaaca
      301 ggcggcgcag gcaacaatcg gaggcagcag gatctgtcgt cgctcttcga ccgcgagctg
      361 gctcgcgagc aggaaaccaa ttacgagact ccaccgggcc gcgaggcgga acaagagcaa
      421 aagtcgggag atgaggcgct cgacaaggtt cgtgatcttg cgcgacggca ggatgggctg
      481 agccgcagcg agaaggagct tgcggagaag cgcaacgccc ttacgcagga gcaggtgaaa
      541 cgcgagctcg agcggcttac gcgtgagcag agcgagttga ggcggcaggc ggaagaactg
      601 gcgcgcgagc ttcaacagaa tcagcaggga cagagcggcc agcaaagcca gggcggccaa
      661 cacagccagg gtggacagca aagccagggc gggcaacagg gccaggccgg gcagcggagc
      721 caggatgggc agcaggcccg gaatggccag gtaggtcaga acgggcaaag tagtgatcgg
      781 cggcaggcga gccgcgcgtt acagcaggcg tccgaggaca tgcagagcgc tgcaagcgag
      841 cttcggcgcg acaacctggg gcaggcgagc gcccgcggca gccgggcggc tgaacggctg
      901 cgcgacgtcg aacagcagat gcgcggatcg cagcccgacg atcggcgtcg tgccctcggt
      961 gagctgcagt tggaatcgcg gcagcttgcc gatgctcagc ggcgcctcgg aagcgattcg
     1021 acagccggag cgggccgttc cggtgatcgc ggcgatcgag cccggcagcg ggcgacggaa
     1081 caggagcgtc ttgccgaccg gacggagcgt ctcgaagaag cggtgcgaca ggtggccggc
     1141 ggcgcgcggg aaggagacca gcgacagcgg aatgcgttga acgaagctgc gcaggatctc
     1201 gaccgacagc ggttgtcgca gcggatgcgt gacgccgccc gtgcggaacg ccaggccagc
     1261 aatgcgcagg gcagcaggca gacatctgcg cagcgcgaag gtgaggcgat tgcgcgtgga
     1321 ctcgaccgtc tggcggatca gctcggcgcc gccggtggcg acagtgaagc atctcagcag
     1381 ctgggcgagg agttgtcgcg tatacgccgt cttcgcgaac agttgacgtc gctcgatcgc
     1441 cagcttgcgg accttcgcaa tgggtcgccg ggtgaatccg gccgtcaggg cggtgagccg
     1501 gggcgtcagg gaggcaatga gcgtggccgt caaggcggcc gaggggcgca acctggtggt
     1561 ctcggacagg acggcggcat ggagggcccg tggcaggagg cccgtgagct gctgaacgag
     1621 ctgcgtcaga acgaagaagt cgttccggcc gacgcagacg ggttcaaccc gggtcgttcg
     1681 gctccgggca cggaagcatg gaagcaggat tttgaaaagt gggatgagct gaaggttcag
     1741 ctggcggcag cgctcgagcg ggtagagcgc acgacggcgg accggcttcg cggccagcag
     1801 tcgcgcgatc gtctcaacgc cggcgcgacg caggcggtgc cggagtcgta tcgccggctc
     1861 gtcgaaaagt actaccgcgc gatcgcttcg ggagagggca ggggaaggta gccggaggcc
     1921 tttaggcctc cggaaagcct ccgcgataga aaatgcaact cgccgtttct tttccgtggt
     1981 ggatgctcct ggggctggcc gttgccaccg cggcggtcgc atgggcatcg tatgccggcg
     2041 cgattgtcgc gctgtcgccg cgccatcggg ctgcgctcgt gtcgctccgc gcgctgacgc
     2101 tggcgcttct ggtgctgtgt ctgctgcgac ccgttcgtgt aattccgccc gacaccacca
     2161 gcgacgctgt cgtcccgatc ctcatcgatg cgtcgcgcag catggcgctt gcggatgtgg
     2221 acggcagccg ccgcctcgat attgcgcaaa aactcgtcca atcggagatt caacccgcgc
     2281 tggagcgccg gttccgccag gagctctgga gcttcggcga cggccttgaa cagatcgccg
     2341 aacgtggcct tgtcgcggat gcacggcgca gcgacctgtc cggcgcgcta agggcggtgc
     2401 gggagcgcta ccgcgaacgg caggtggcag gtatcgtggt gatttccgac ggcggggaca
     2461 ccggatccca ggatgccgca gcggccgtcg aggaaggtgg agcgcctgta tacacgattg
     2521 gtgttggcgc tccgcgcgta acgccggatc tcgaggtgct cgatctctcc gcgggtgacg
     2581 ccgcggttgc agactcgtcg atcgatatca gcgtcgcggc ggtcagccgg ggcggcacgg
     2641 ctcctttcga tttgcgcgtg ctcgccaacg ggaggccggt cgatttacga cgcgtcacgc
     2701 cggcagcgga cggcagtcca gtgcgggccg tctttacggt gacgccggcg cgtgaaaccg
     2761 cgacgctcta caccgtcgag attccgtctg gcgcgggcga gcgcgtgctc gaaaacaatc
     2821 gccgcagcgt actcgtcgag ccgccgggca gggtccgccg gattctgatg atcgaaggaa
     2881 cgcccggttt cgagcactcg ttcatcaagc gggcgttgag caccgatccg gggttcgaga
     2941 tcgattcggt ggtccgaaaa gggcgcgacg cgcgcggtgc cgccacatat ttcgtactcg
     3001 ccacgccgac gcgcgcgccg cgtctggctt cggggttccc tcagcagcgc gccgcgctgt
     3061 acgagtacga cgcgatcgtt ctggccaacg ttgagcccga tacgctctcg cgggcgcaac
     3121 tccagttgct cgcggatttc gtcgaacagc gcggtggtgg gctgctcgtg ctcggaggaa
     3181 agtcgtttgc ccagcagggt tttgccggga caccactcga agaagcactt cccctgaggc
     3241 tgaccgaccg cggaaatgga gtcgtgcgcg ccgcgaaccg ccccgaatct cgcttgaccg
     3301 tcaggctgac ggcggacggg cttacgcacc ccgtaatgcg caccgacggc accgccgagg
     3361 gtctggatag gcgctggcag gcgatgcccg cgctggccgg cgtggcaagc ctcggtgcgc
     3421 tgcggccggg cgcgcaggcg ctggcggtgg tgaacgcggc cgacggtcca cgccctctcg
     3481 tagcggtgca gcgttacgga caaggtcgcg tcgtcatatt cgcgggcgag gcttcctggc
     3541 gatggcgtat gcagatgccg tcgaccgatc gcacgcacga gttgttctgg cgtcaggcgg
     3601 tgcgctggat gtcttctccg gctccggatc gggtatcgat tggtacggtg ccgtcactcg
     3661 tgccgggcag caccgccgcg atcagtgtcg ctgttcgaac cgaggagttt gcgtctgtgg
     3721 gcgacgccag cgttcggctg cgggttacgc ggccggatgg gtcggcccaa gacgttgccg
     3781 cggcattggc cgattcgcaa acgggcagct acctgggcga agtgccgctc gaggaacccg
     3841 gagtctaccg
//
LOCUS       DBNGSG010000115         3030 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_889017, whole genome shotgun sequence.
ACCESSION   DBNGSG010000115 DBNGSG010000000
VERSION     DBNGSG010000115.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_889017"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..574
                     /locus_tag="AC158Q_02960"
     CDS             128..574
                     /locus_tag="AC158Q_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HIR3338757.1"
                     /translation="MSLKARINDDVKTAMRGGDARRRDALRLLLSALKQREVDERKEL
                     ADADVVAVIEKMIKQRRESITQFEKGGRQDLAQNEQFEVETFQAYMPQALSGAEIEAT
                     VAEAIATTGATSPSDMGKVMAVLKGKLAGRADMSKVSAMVKSKLSG"
     gene            757..2529
                     /gene="dnaG"
                     /locus_tag="AC158Q_02965"
     CDS             757..2529
                     /gene="dnaG"
                     /locus_tag="AC158Q_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008325809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HIR3338758.1"
                     /translation="MIPQSFIQDLLGRVDIVEVVDRHVKLKRAGANYVACCPFHSEKS
                     PSFTVSPTKQFYHCFGCGAHGTAVGFLMEHSGMGFVEAVKDLAQSVGMKVPEEPRSES
                     AQRRAQQGESLHEVLLRAAQHYRNQLKDAPQAIAYLKKRGLSGDIAKRFGIGYVPDEW
                     QNLAAAFSEYDGPMLAAAGLVKSKEDGKRYDVFRNRIMFPIVDVRGNVIGFGGRVLGE
                     GEPKYLNSPETPVFEKGRELYGLYQARRAIHDAGRAVVVEGYMDVVALAQSGVEYAVA
                     TLGTATTPLHVQKLLRQTDEIVFCFDGDNAGRRAAWRALENSLALLSDGKQLKFLFLP
                     EGEDPDSYVRQHGRNAFESLLDKGAVPLSRFLLDELTGRVDLATAEGRAKCVHEAKPL
                     LKQMPANALRLQLTRELAEKCRLSPEEVSQLCELSASAPAGPRHAEAPRAPRKPVTPL
                     AEQLLRLLVLSPGLAHSLSAEHRALLELPEMAPVVELIEAVLESGATSSAMLFEATRE
                     SQYAESYQDIAARSLTDAADDATAKTILSDVLLKLERQRMESEFEKLSAGGQRSDVER
                     QRFQEVTRRLAELKKATTGSRAEI"
     gene            2718..>3030
                     /locus_tag="AC158Q_02970"
     CDS             2718..>3030
                     /locus_tag="AC158Q_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338759.1"
                     /translation="MAKSTKSRSRAKAAARGKTHAKARTAAKRHAAKPKAKSAKLQAR
                     DRHVAPKVKPAPKELRTVVKPLPKGAVPPKGAKVAQKMDKAALKKALLDERIAKAQAK
                     KA"
BASE COUNT          603 a          941 c         1002 g          484 t
ORIGIN      
        1 catcacaaga agctgcgcgc gcagctgctg ccgccgcgga tgtattgaca ccccgcgagg
       61 ggtgcagcac aaggcgtgag gggaagtccc tcgcgccttt tgtttttcat cactcataac
      121 ccatcacatg tccctcaagg cccgcatcaa cgacgacgtc aaaaccgcca tgcgcggcgg
      181 cgacgcgcgc cgccgtgatg cgctacggct gctgctctcg gcgttgaagc agcgcgaagt
      241 ggacgagcgc aaggagcttg cggatgcgga cgtggtggcg gtcatcgaaa agatgatcaa
      301 gcagcggcgc gagtcgatca cccagttcga gaagggcggc cgccaggacc tggcgcagaa
      361 cgagcagttt gaggtcgaga cgttccaggc ctatatgccg caggccctgt ccggcgccga
      421 aatcgaggca acggtcgcgg aggccattgc gacgaccggc gccacgtctc cttccgatat
      481 gggcaaagtc atggctgtgc tgaagggcaa gctcgccgga cgggccgata tgtccaaggt
      541 atcggcgatg gtgaaatcca agctttcggg ctgatcgagg acgctgcggc ccgtccgaac
      601 gggtctccgc attggcagaa taattgcact gaacgcgggt cacccgtccg cagttgtttt
      661 ccctggccca ccccggaaat aggggtctat actttcaata taaatcatgc gcgcaattaa
      721 cccgcccgtt cacctcccgc agcgttcctt gcgcgcatga ttccgcagtc cttcatccag
      781 gatctcctcg gccgggtcga catcgtcgag gtggtggacc ggcacgtgaa actcaagcgc
      841 gccggcgcga attacgtcgc gtgctgcccg ttccacagcg agaaatcacc ctcctttacc
      901 gtgagcccga ccaagcagtt ctaccactgc ttcggctgtg gcgcgcacgg cacggcggtc
      961 ggcttcctga tggagcacag cggcatgggc ttcgtcgagg cggtgaaaga cctcgcgcaa
     1021 agcgtcggta tgaaagtgcc ggaggagccg cgttccgaat cggcgcaacg cagggcccag
     1081 cagggcgaga gcctgcacga agtgctgttg cgggcggccc agcattaccg caaccagttg
     1141 aaggacgcgc cgcaggccat cgcctacctc aagaagcgcg ggctgtccgg cgacatcgcg
     1201 aagcgattcg gcatcggcta cgtccccgac gaatggcaga acctcgcggc ggccttttcg
     1261 gagtacgacg ggcccatgct ggctgccgcg gggctggtga agagcaagga ggatggcaag
     1321 cgctacgacg tgtttcgaaa ccgcatcatg ttcccgatcg tggacgtgcg cggcaacgtg
     1381 atcggcttcg gcgggcgcgt gctcggggag ggcgagccca agtacttgaa ctcgccggag
     1441 accccggtat tcgagaaggg gcgcgagctc tacggcctct accaggcgcg gcgcgcgatc
     1501 cacgatgccg gccgcgcggt ggtggtcgag ggctacatgg atgtcgtcgc gctcgcgcaa
     1561 tccggggtcg agtatgcggt tgcaacgctg ggcaccgcga ccacaccgct gcacgtgcag
     1621 aagctgctgc gccagaccga cgagatcgtg ttctgcttcg acggcgacaa cgcgggccgg
     1681 cgcgccgcgt ggcgcgcatt ggaaaacagc ctcgccctgc tctccgacgg caagcagttg
     1741 aagttcctgt tcctgcccga gggcgaggac ccggactcct acgtgcgcca gcacggcaga
     1801 aacgcgttcg agagcttgct cgacaagggc gccgtcccgc tgtcccgctt cctgcttgac
     1861 gagctgacgg ggcgcgtcga tctcgccacg gccgagggcc gcgccaaatg cgtgcacgag
     1921 gcaaagccgt tgctgaagca gatgccggca aacgcgttga ggttgcagct cacgcgcgag
     1981 ctggcggaga aatgccgctt gtcgccggaa gaggtcagcc agctctgcga gttgtcggcg
     2041 tccgcgccag ccgggccgcg tcatgccgaa gcgccgaggg ctccgcgcaa gccggtcacg
     2101 ccgctcgcgg aacaactgtt gcggttgctc gtcttgagtc cgggcctggc gcactcgtta
     2161 tccgccgagc accgggcgct gctggaattg cccgaaatgg cccccgtcgt cgaattgatc
     2221 gaggccgtcc tggagagcgg cgccacctcc tcggccatgc tcttcgaggc gacgcgggaa
     2281 tcgcagtacg ccgagtccta ccaggatatc gcggccagga gcctgacgga tgccgctgat
     2341 gacgcgaccg ccaagaccat tctttcggac gtgctcctca agctggagag gcagcggatg
     2401 gagagcgaat tcgaaaagtt gagcgccggc ggccagcgta gcgatgtcga gcgccagcgc
     2461 tttcaggagg ttacccggcg gctggccgaa ctgaaaaagg ccacgacggg cagccgcgcc
     2521 gagatttgaa tggaatcacc gattgaagtg ttagccccgc aaccccactt acttcatacc
     2581 acccgttcta ccggcctggc ttggaaattg cttatgcgac aaacagttgg gtggtaactt
     2641 gcgggtgtat gcggggtcga acaagggtat aatccgcagc tttttttgat gcagcaattt
     2701 cacgcgggaa cagcgaaatg gcgaaatcca ccaagtccag gtccagggcg aaagccgcag
     2761 cgcgcgggaa aacgcatgcc aaggcgcgga ctgccgcgaa acggcatgcc gccaaaccaa
     2821 aagccaagtc tgccaagctg caggccaggg accggcacgt cgcgccgaag gtgaaaccgg
     2881 cgccgaaaga gctgcgaacc gtggtcaaac ccctgcccaa gggcgcggtc ccgcccaagg
     2941 gggccaaggt cgcgcagaag atggacaagg cggcgctgaa aaaggcgctg ctggacgagc
     3001 gcatcgccaa ggcgcaggcg aaaaaggcgg
//
LOCUS       DBNGSG010000116         1746 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_209677, whole genome shotgun sequence.
ACCESSION   DBNGSG010000116 DBNGSG010000000
VERSION     DBNGSG010000116.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_209677"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(536..1675)
                     /locus_tag="AC158Q_02975"
     CDS             complement(536..1675)
                     /locus_tag="AC158Q_02975"
                     /inference="COORDINATES: protein motif:HMM:NF013567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3338760.1"
                     /translation="MAKHDETIRSARAIDSQIIDSGRRTLLLAAGAGALAAAIGGRGE
                     AQGMKSLRWGVVGTGGIANGMAPRILEAEYAELAAVSSRRMETAQEFADQHGAGRAFD
                     SWQEMIAWDGIDAVYIATPTSVREEIGVAAANAGKHVLGEKPFANLPSLRRITAACRA
                     NGVGFMDGTHFPHEPRSAHIRARMQQDLGWPWSVASAFQFNLSDTSNIRLQPELEPYG
                     AIGDTGWYNMRAAVEYTAPGVAIAAVDAYLRRHPETKAVVSASGVIRFDDGSTSTFNC
                     GFDSGAGVQDLRISGTKAVVKLDDFLRSRRDDHTGVYEYRSGWDERKQVEVTSAKPGA
                     TLMFEDFAAMVGDPGRLEASIRASERTQEWLDAVWESALENERGG"
BASE COUNT          283 a          566 c          574 g          323 t
ORIGIN      
        1 ctgcacgtct tccactttct tgccttcatt gagcttgcag gtgtaggcgg cgacgtaatc
       61 atcggcggac agcgagctgc tggcgacagc ggcggccgac gccaacagta cgatcgaaac
      121 aaagttcttc atgttgcact cctcagttgg ggatcttgct tggggttttc ttgtcgtgca
      181 gctgcagtat agatggttat ccgcggcttg cgctggctgg cgctggacga tgttaccgcg
      241 gaacgtcatt atgttaaatg atggatctcc cgagggtcgc atattgccag tcccccgccg
      301 gcaaatgtac tcttttccct gaaccctcaa acgtggtgtc gacccggcac caggagcaca
      361 gaccatgagc aacaaggaac agcacggcaa caaggaaagc aagaagaaac cggcgttgac
      421 gctgaaagaa aagaaggcgc tgaagcgcgc caagaagcag gccaagggag ggtagtaccg
      481 acgccgtcga gacttgattt tccggtaacg cgaaccggct gagcggtgtg gcctctcagc
      541 ccccgcgttc attctccagc gcactctccc acaccgcatc cagccactcc tgggtgcgct
      601 cgctggcgcg gatgctggcc tccagccgcc cggggtctcc gaccatggcg gcgaagtcct
      661 cgaacatcag cgtggcgccg ggcttggcgc tggtcacctc gacctgtttg cgttcgtccc
      721 agccgctgcg gtattcgtag acgccggtgt ggtcgtcgcg gcgcgagcgc aggaaatcgt
      781 ccagcttgac caccgccttg gtgccggaga tccgcaggtc ctgcacgccg gcgccggagt
      841 cgaaaccaca gttgaaggtg ctggtggagc cgtcgtcgaa gcggatcacg ccgctggcgc
      901 tgaccacggc cttggtctcc gggtggcgcc gcaggtaggc gtcgaccgcc gcgatggcga
      961 cgcccggcgc ggtgtactcc accgcggcgc gcatgttgta ccagccggtg tcgccgatcg
     1021 cgccgtaggg ttcgagctcc ggctgcaggc ggatattgct ggtgtcgctg agattgaact
     1081 ggaaggccga ggcgactgac cagggccagc cgaggtcctg ctgcatgcgt gccctgatgt
     1141 gcgcgctgcg cggctcgtgc ggaaaatgcg tgccgtccat gaagccgacg ccattggcgc
     1201 ggcaggcggc cgtgatgcgc cgcagcgacg gcagattggc gaagggcttc tcgccgagca
     1261 cgtgcttgcc ggcgttggcc gccgccacgc cgatctcctc gcgcacgctg gtcggggtcg
     1321 cgatgtagac ggcgtcgatg ccatcccagg cgatcatctc ctgccaggaa tcgaaggcgc
     1381 ggccggcgcc gtgctggtcc gcgaattcct gcgcggtctc catgcggcgg ctggacaccg
     1441 cggccagctc cgcgtattca gcctcgagga tgcgcggcgc catgccgttg gcgatgccgc
     1501 cggtgccgac gacgccccag cgcaggctct tcatgccctg tgcctccccg cgccccccga
     1561 tggctgccgc cagcgcgccg gcgccggccg cgagcagcag cgtgcgccgc ccggaatcga
     1621 tgatctggct gtcgatcgcc cgcgcggagc ggatggtttc atcgtgcttg gccatgtgtt
     1681 tctcccgttc attcagttct cctgcaagaa ggcgttgacg atcgcctcca ctttccaggc
     1741 ttcgca
//
LOCUS       DBNGSG010000117         4380 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3775957, whole genome shotgun sequence.
ACCESSION   DBNGSG010000117 DBNGSG010000000
VERSION     DBNGSG010000117.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4380)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3775957"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="AC158Q_02980"
     CDS             <1..456
                     /locus_tag="AC158Q_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338761.1"
                     /translation="IPSWEMSRSLIHNPMVPIHEFGHVLFRPFGEFMHLLGGSLFQAG
                     LPLIFGGIFLVKNRDPYAAAVMLWWSAVAVMDIAPYAYDAHMPQHILLSGRTGDTGSH
                     DFIDVLYDLGLLKQAQVVGYAMHRFGVAMLLASFVWAAWVVWKQNAVKR"
     gene            639..1838
                     /locus_tag="AC158Q_02985"
     CDS             639..1838
                     /locus_tag="AC158Q_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HIR3338762.1"
                     /translation="MRDHSSITLTGGRFDWSDPLLIERQLTAEERQIRDAARAYAQAK
                     LAPRIRDWNRSESFDRAVMREMGELGFLGAPLEGYGCAGIGYVAFGLVMRELERVDTA
                     FRSACSVQTALAMTPIHAYGTEAQRNQYLPKMATGEILGCFGLTEPDHGSDPGSMTTR
                     AKKCDGGYLLTGTKLWITHAPIADIMVVWAKDDGGVIRGFILERGMKGLATRKIEGKF
                     SVRASPTGEIVMDQVLVPEANLLPGAQGLKGPFGCLNNARFGICWGALGAAEACWHTA
                     RQYVMDRKQFGRPLAANQLIQKKLADMQTEIAIGLLACLHVSRLREQRLATPEMVSLL
                     KRNSTGKALEIARLARDMLGGNGISDEFPVIRHLLNLETVNTLEGTHDVHALVLGRAQ
                     TGISAFA"
     gene            1977..2165
                     /locus_tag="AC158Q_02990"
     CDS             1977..2165
                     /locus_tag="AC158Q_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338763.1"
                     /translation="MLHRPLTERDMALISVLCRDREVRCEQRIVAAVKAGNKELEQVA
                     LADQERYQSLRLKMFFQS"
     gene            2219..3187
                     /locus_tag="AC158Q_02995"
     CDS             2219..3187
                     /locus_tag="AC158Q_02995"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3338764.1"
                     /translation="MNAQRVLAAIAAVLISAPAAQAAQSGFPDKPVRLIIGSAPGSGP
                     DIIARLVADHLYGAWGQRVVVDSRPGVAGVISAELSLRANPDGYTWMMLTSQLFVATS
                     VYPDLKFDLDKDFVSIALIGTVPFVQMVNPQLPVKSIADLVALAKKSPGKLRYGSAGA
                     GASEHLSGVLFNRLSGTDMLHVPYKGIAQAILDTIGNEVQLTYAVYPAARPHVESGRL
                     LAIGVTTPKRSPALPNVPAIAETLPGYAMFGWYSLVAPKGTPNAVLAKTSDEVLRAVK
                     DPAFGNRLKTLGIEVVGGGRKDLDTFRRNERKRVSELVKAAGVTIK"
     gene            complement(3325..3687)
                     /locus_tag="AC158Q_03000"
     CDS             complement(3325..3687)
                     /locus_tag="AC158Q_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338765.1"
                     /translation="MLIMAAWITAFKAIPWTVLIAAAPSIVDGARKLWDAVKKQEAPR
                     VSGQGQVADQRALELQVAELRAELAAASELVTRLAEQNTRLVEAVDVLRVRTRLLLAA
                     TAVMAVVLFGLLLAVALG"
     gene            3852..4241
                     /locus_tag="AC158Q_03005"
     CDS             3852..4241
                     /locus_tag="AC158Q_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338766.1"
                     /translation="MRSIAPVQYPDITFSGKSGEKYRFQAWSLETRFKPMAAVYFVTK
                     RAQDSSTYNRASHDSVYIGQTANLADPFGTESQFDCFRKHGANCICIRLVESEEQRIA
                     VEEDLIALHSTHCNQLARIARVFDTAE"
BASE COUNT          838 a         1390 c         1468 g          684 t
ORIGIN      
        1 attccgtcgt gggagatgtc gagatcgctc atccacaatc cgatggtgcc gatccacgag
       61 ttcgggcacg tgctgttcag gccgttcggc gagttcatgc atctcctcgg cggctcgctg
      121 ttccaggccg ggctgccgct catcttcggc gggattttcc tggtgaagaa ccgcgatccc
      181 tacgccgccg cagtcatgct atggtggtcc gccgtggcgg tgatggacat cgcgccctac
      241 gcctacgacg cgcacatgcc ccagcacatc ctgctctcgg gccgcaccgg cgacaccggc
      301 tcgcacgact tcatcgacgt gctgtacgac ctcggcctgc tcaagcaggc gcaggtcgtg
      361 ggttacgcca tgcaccgctt cggcgtcgcg atgctgctcg cgtccttcgt ctgggcggcg
      421 tgggtggtgt ggaaacagaa cgccgtcaag cgctagagac agggatgagg gaaggagtgt
      481 cgagggaaga aacgagggac gaggattgag tgtcgagtga acaaaacgtt acagcgcttt
      541 acgcctcgac actcgacacc cgacgcccga cacccgaccc tcgacacccg acacccgaca
      601 cccgacctcg accctcgaca ctgaaaacgg ggaagaaaat gagagaccac agttcgatca
      661 ccctgaccgg cggccggttc gactggtccg acccgctgct catcgagcgg cagttgaccg
      721 cggaggaacg gcagatccgc gatgccgccc gcgcctacgc gcaggcgaag ctcgcgccgc
      781 gcatccgcga ctggaaccgc agcgaatcgt tcgaccgggc ggtgatgcgc gagatgggcg
      841 agctgggctt tctcggcgcg ccgctggaag gctacggctg cgcgggcatc ggctacgtcg
      901 cgttcgggct cgtcatgcgc gagctggagc gggtggacac ggcattccgc tcggcgtgca
      961 gcgtgcagac ggcgctggcg atgacgccga tccacgctta cggcaccgag gcgcagcgca
     1021 accagtacct gccgaagatg gcgacggggg agatcctcgg ctgcttcggc ctgaccgagc
     1081 ccgaccacgg ctccgatccc gggagcatga ccacgcgcgc gaagaagtgc gacggcggct
     1141 acctgctcac cggcaccaag ctgtggatca cccacgcgcc gatcgccgac atcatggtgg
     1201 tgtgggcgaa ggacgacggg ggcgtgatac gcggcttcat tctcgagcgc ggcatgaagg
     1261 ggcttgcgac gcgcaagatc gagggcaagt tctcggtgcg cgcatcgccg accggcgaga
     1321 tcgtgatgga ccaggtgctg gtgccggagg ccaacctgct gccgggggcg cagggattga
     1381 agggcccgtt cggctgcctg aacaacgcgc gcttcggcat ctgctggggc gcgctgggcg
     1441 ccgctgaggc gtgctggcac acggcgcggc agtacgtgat ggaccgcaag cagttcggcc
     1501 ggccgctcgc cgcgaaccag ttgatccaga aaaagctcgc ggacatgcag accgagatcg
     1561 ccatcgggct gctcgcgtgc ctgcacgtga gccggctgcg cgagcagcgg ctcgcgaccc
     1621 cggaaatggt gtcgctgctg aagcgcaact ccaccggcaa ggcgctcgag atcgcgcggc
     1681 tcgcccgcga catgctgggc ggcaacggta tctccgacga gtttcccgtg atccgccacc
     1741 tgctgaacct cgagaccgtc aatacgctcg agggcacgca cgacgttcac gccctcgtgc
     1801 tcgggcgggc gcagacggga atttcagcgt tcgcctagcg gacacggacg ggcgaagtcg
     1861 aaaggcgaaa ggcgaaagta agaccgcttt ttccttggtg taccggtttc ctgtaggatg
     1921 gccgcttcgg cgccgcgcct tcggtttccg gcgccggccc gcgatcgagg tgactcatgc
     1981 tgcacagacc cctgacagaa cgagacatgg cgctcatttc cgtgttgtgc cgggatcggg
     2041 aggtgcgctg cgagcagcgc atcgttgccg cggtgaaggc cggcaacaag gagctggagc
     2101 aggtggccct ggcggaccag gagcgctacc agagcctgcg cctgaagatg ttttttcagt
     2161 cttgagcggt ggcggaacca ggcagtcaaa acagcaggac aaccagagga gacaggagat
     2221 gaatgctcag agagtgttgg cggccattgc cgcggtactg atttcggcac cggcggcgca
     2281 ggcggcgcag agcgggtttc ccgacaaacc ggtccggctc atcatcggca gcgcgccggg
     2341 atcgggcccg gacatcatcg cgcggctcgt ggccgaccac ttgtacgggg catggggcca
     2401 gcgggtcgtg gtggattccc gtcccggcgt ggccggcgtc atcagcgcgg aactctccct
     2461 gcgcgcgaat cccgacggct acacctggat gatgctgact tcgcagctgt tcgtcgcgac
     2521 ctcggtctat cccgacctca agttcgacct ggacaaggat ttcgtctcga tcgcgctgat
     2581 cggcacggtg ccgttcgtgc agatggtgaa cccccagttg ccggtgaaat ccatcgccga
     2641 cctcgtggcg ctcgcgaaga agtcgcccgg gaagctgcgc tacggctcgg ccggcgccgg
     2701 cgcctccgag cacctctcgg gcgtgctctt caaccgcctg agcggaaccg acatgctgca
     2761 cgtgccgtac aagggcatcg cccaggccat cctggacacg atcggaaacg aggtgcagct
     2821 cacctacgcg gtctacccgg ctgcccggcc tcacgtcgag agcgggcgac tgctggcgat
     2881 cggagtcacc acgccgaagc gctcgccggc gctacccaac gtgccggcca tcgcggaaac
     2941 gctgccgggc tacgccatgt tcgggtggta cagcctggtg gcgcccaagg gcacgcccaa
     3001 cgcggtcctc gccaagacca gcgacgaagt gttgagggcg gtcaaggatc ccgcgttcgg
     3061 caaccggctc aagaccctgg gcatcgaggt ggtcggcggc ggccgcaagg acctcgacac
     3121 gttccggcgc aatgagagga agcgggtgag cgagctggtg aaggcggcgg gcgtcaccat
     3181 taagtaacaa gtcgaaagtc gaaaggcgaa agaaaccgaa ccccggtgac gagtgacgag
     3241 ggctgcgtcg aatcacgaac ccccacccca accctccccc gtgggaaggg ggaggcgcag
     3301 gagggggtta cgacacacga atcactaccc cagcgcgacg gcgagtagaa gcccgaacaa
     3361 cacgacggcc atgacggcgg tcgccgcgag cagcaagcgg gtgcgcacgc gcaggacgtc
     3421 gacggcctcg accagccggg tattctgctc ggcgagccgg gtgacgagct cggaggcggc
     3481 ggcaagttcc gcgcgcagtt ccgcgacctg cagttccagc gcccgctggt ccgccacttg
     3541 cccctgtccg ctcacgcgcg gcgcctcctg ctttttgacg gcatcccaga gcttgcgcgc
     3601 gccgtccacg atcgacggcg cggccgcgat cagcacggtc cagggtatgg ctttgaatgc
     3661 ggtgatccag gcggccatga tgagcaccca aaatgagtga tgagtgatga gtgatgagcg
     3721 acgagtggcg agcgacgagt gtacacccgg ccagaagact ggcgaaaggc gaaaagagct
     3781 ctgtttggag ggattaatgt ccggctttcg attttcacct tgccgcgtaa cgctcgctat
     3841 tggggcggaa tgtgcgtagc atcgcccccg tgcaataccc ggacatcacg ttcagcggaa
     3901 aatcaggcga gaaataccga ttccaggcct ggtccctgga aacgcgcttc aagcccatgg
     3961 cggccgtgta cttcgtgacg aagcgcgcgc aggacagcag cacctacaac cgggcttccc
     4021 acgacagcgt ctacatcggt cagaccgcga acctggcgga cccgttcggc accgaatcgc
     4081 agttcgactg ttttcgcaag cacggggcga actgcatatg catccgcctg gtcgaaagcg
     4141 aggaacagcg catcgcggtg gaggaagacc tgattgcgct gcacagcacc cactgcaacc
     4201 agctggcgag aatcgcccgg gtattcgaca cggcggagta gacgctgcgc ttgaaggcga
     4261 aagtcgaaag gcgaaaagcg aaagtgcctg gaacccggtt gacgagaaac gagcagcgaa
     4321 tcacgcagtt tcagttgccc cccagcagtc ccagcgtttc gaacttcaag tacagctcgc
//
LOCUS       DBNGSG010000118         9540 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_889822, whole genome shotgun sequence.
ACCESSION   DBNGSG010000118 DBNGSG010000000
VERSION     DBNGSG010000118.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9540)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9540)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9540
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_889822"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="AC158Q_03010"
     CDS             complement(<1..494)
                     /locus_tag="AC158Q_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338767.1"
                     /translation="MFNAIRSFPAVALVAALAAFVAGSALAQSKGSGTIVCWKDQSGK
                     TIGCGDKVPPEYQDNATKELDRRGVTRRTTETAEERAKRVALEKEQAAKQVEEKKRIA
                     EQKRQDAALINTFSNAQEIDLKRDRDLQVADTQMVQLRVAHKNAMGREKEVRARIQNA
                     KGTPS"
     gene            complement(487..1140)
                     /gene="pyrE"
                     /locus_tag="AC158Q_03015"
     CDS             complement(487..1140)
                     /gene="pyrE"
                     /locus_tag="AC158Q_03015"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HIR3338768.1"
                     /translation="MTSDFRQEFIRFTIRQNVLRFGEFKTKAGRLSPYFFNAGLFYDG
                     EALKQLAQFYAKAILAADTGFDMLFGPAYKGIPLVAAVSIALADAGRNVRYSFNRKET
                     KDHGEGGAVIGAPLSGRVLIVDDVISAGTSVRESVNLIRGSNAIPCGVAIALDRMERG
                     SGALSAVQEVRASYNIPVISIATLDDLMAYLRADPQQEQYLAAVGRYREQYGVSAHV"
     gene            1237..2016
                     /locus_tag="AC158Q_03020"
     CDS             1237..2016
                     /locus_tag="AC158Q_03020"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HIR3338769.1"
                     /translation="MRIITLNLNGIRSAARKGVFRWMARTRADVVCVQELKAQAADLE
                     EEMRAPGGFHGFFHHAEKKGYSGVGLYSRREPDRVVQGIGDNEADCEGRYVRADFGKL
                     SVVSVYLPSGSAGEHRQAAKFRFMECFFPFLKRLASEGREILLCGDWNIAHREIDLKN
                     WRSNRKNSGFLPEERAWLTRVFDELGWVDVYRRLYPDATGEAYTWWSNRGQAYANNVG
                     WRIDYHIATPGLAAAAKRAAIYKTRRFSDHAPLTIDYDFGS"
     gene            2016..3293
                     /locus_tag="AC158Q_03025"
     CDS             2016..3293
                     /locus_tag="AC158Q_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HIR3338770.1"
                     /translation="MAGSPIPAASARPPWRDTLAAIFSGRMLYALLMGFASGLPLLLT
                     GSVLQAWLKRSGIDLTSIGLFALVGLPYTLKFLWAPLFDRYLPPLFGRRRGWLLAIQA
                     LLAAALFALSFAHPAPDGLLAVSVAALLVAFFSASQDIVIDAYRRESLADVELGFGSA
                     MYVNGYRIGLLVAGAGGLILAGSYSYETMYCVMAAVMAACIAVTLAAPEPPLPAGRPV
                     TLAEAVFLPFRDYFTRGGAWLALAFILLYKLGDTMASAMTTPFYLDRGYSETEIGTVV
                     KLFGFWATIAGGTLGGVWILRIGMSRALWLFGLGQMLSTLGFVVLAGVAPTAAALATV
                     VAIENFTAGLGTAAFVGFMASLTDRRFTATQYALLSSLMGVPRVFASAPTGWFAELMG
                     WPGFFLMCTLIAIPGLLLLRWIARLADGAGSSR"
     gene            3361..4254
                     /locus_tag="AC158Q_03030"
     CDS             3361..4254
                     /locus_tag="AC158Q_03030"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR3338771.1"
                     /translation="MAQTEPLSVHTGGTEIPVPRDFRWPGGKRIAVIFRVAFEAWSDD
                     HWPGISPMGNPLKPGFPDLNARGWADYAYRRGVFRALDALAHHRVKATFMVCGILAER
                     FPHIVKQIADAGHEVAAHSYTMNMMSVYMSEEEERANIRHNVELIERATGRRPVGWIN
                     PRGTPGPNTARLLAEEGFAWHGDTLNDDLPYVVKFGSRSIVAVPSNMDVNDLPLYMKH
                     GQPPGVFVENFEGFLRYAQKREKGASKIDPGIHCHVFGRPAGMWAFERVLEIAKKSKG
                     VWIGTRAEAATHVRKVLKTPN"
     gene            4559..5455
                     /locus_tag="AC158Q_03035"
     CDS             4559..5455
                     /locus_tag="AC158Q_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020832007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR3338772.1"
                     /translation="MRLVTFVPKKGAAPRLGVVRDGSQVVDLTPGSGKAPFDPCDMIS
                     LIASGAKGLAWAKQAAARVKKTLPLAKVRLLAPIPRPRKNVFCVGWNYVEHFEEGKKA
                     LKHTQELPAHPAFFSKVPTTVIGPHDRVPFDPAVNKTLDWEAELGVIIGRGGRNIAEA
                     DAMKHVFGYTVINDVSVRYIQRQHGGQWFKGKSVDGTCPMGPWIVTADEVPDPHALRI
                     GCRVNGNTKQDSNTSYMYFKVPRIIAELSYGLTLEPGDIISTGTPAGVGHARTPPEFM
                     TAGDVLETEVEGLGLLRNKIGN"
     gene            complement(5638..6240)
                     /gene="metW"
                     /locus_tag="AC158Q_03040"
     CDS             complement(5638..6240)
                     /gene="metW"
                     /locus_tag="AC158Q_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HIR3338773.1"
                     /translation="MRTNGNGFKRADFDAIAESIRPGSRVLDLGCGDGVLLKYLKGAR
                     AVTGYGVEIDDASVLGCVTNGVDVIQSDLERGLSEFESGSFDYVVLSQTLQAMKNSER
                     IINDMLRVGREGIVTFPNFGYWKNRLQVLAGHMPVSDNLPYQWHDTPNVHLCTISDFE
                     DFCAGHGVRILERKVLTNARSVAVLPNLLGALAVYRFQRA"
     gene            complement(6378..7592)
                     /gene="metX"
                     /locus_tag="AC158Q_03045"
     CDS             complement(6378..7592)
                     /gene="metX"
                     /locus_tag="AC158Q_03045"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase MetX"
                     /protein_id="HIR3338774.1"
                     /translation="MDTGIASKSVGVVTPQTARFTEPLRLRSGAVMDAYELVYETYGK
                     LNADRSNAVLVCHALNAAHHVAGHYADAPDNIGWWDNMIGPGKPVDTSRFFVIGVNNL
                     GGCHGSTGPMSVNPKTGKPWGADFPVVTVEDWVATQARLADHLGIRRFAAVMGGSLGA
                     MQALAWTYLHPGRVRHALVIACAPNLSAQNIAFNEVARQAIITDPDFHGGHYYGHNTR
                     PTRGLRVARMVGHITYLSDDEMASKFGRQLKNGKLNYTFDTEFEIESYLRHQGGKFSE
                     YFDANTYLRITKALDYFDPALEHGGNLARALSPARANFLVVSFTTDWRFSPQRSREIV
                     KALLDNRCGVSYAEINAPHGHDAFLLEDARYHNLVAAYFERIGRERPLSPEAGAPVSG
                     DATRANAAAADT"
     regulatory      complement(7645..7722)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            complement(7856..>9540)
                     /gene="ptsP"
                     /locus_tag="AC158Q_03050"
     CDS             complement(7856..>9540)
                     /gene="ptsP"
                     /locus_tag="AC158Q_03050"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006713308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HIR3338775.1"
                     /translation="AIGQAQLMTHTGLEVQRYLVPPHQAPEESSRFDAAVRQVRAEFE
                     ELHASVPSTAPAEFAAFINLHLMILNDSTLSVAPRRIIETEGCNAEWALKVQTDALLA
                     QFDEIEDDYLRERRADVIQVAERVMKALSGQPGYVPPPRPETGPNVILVAHDLSPADV
                     VQFKRHQYASFITDLGGTTSHTAVVARSLAIPSIVALHRARQMIRENEVIIVDGSQGV
                     VIVDPDQQVLAEYQERQQQFDLERQKLRHLKDRRAVTRDGVPVELQANIELPADVADA
                     RGNGATGIGLFRSEFLFLNRADLPDEDEQFEAYRKVAEEMEGLPVTIRTYDLGGDKDD
                     ENERVTTNPALGLRAIRLCLTEPQRFVTQLRALLRASRHGRINILVPMLSTFAEIDQT
                     LALIRRAKHGLDEEGVPYDHGVKVGGMIEVPAAALALGMFIRKLDFLSIGTNDLIQYT
                     LAVDRTDDTVSHLYDPLHPAVLNLIAGIIRAADRAKIPVALCGEMAGDTTLTRLLLAF
                     GLRQYSMHIAHLLDVKQVILNSSFAELQPLARKMLRATDPARLRALLEKLNS"
BASE COUNT         1567 a         3000 c         3193 g         1780 t
ORIGIN      
        1 gaaggggtgc ccttggcgtt ctggattctc gccctgactt ccttctcgcg gcccatcgcg
       61 ttcttgtgcg caacccgcaa ctggaccatc tgggtgtccg caacctgcag gtcgcggtcg
      121 cgcttcaggt cgatttcctg cgcgttggag aaggtgttga tcagcgcggc gtcctggcgt
      181 ttctgctccg cgatccgctt cttttcctcg acttgtttcg ccgcctgttc cttttccagg
      241 gccacgcgct tggcgcgttc ctcggcggtt tccgtggtcc tgcgcgtaac gccgcgccgg
      301 tcgagctcct tggtggcgtt gtcctggtat tccggcggca ccttgtcgcc gcagccgatg
      361 gtcttgcccg actggtcttt ccagcagacg atagttccgc tgcccttgga ttgggccaac
      421 gcgctgccgg cgacaaaggc cgccagcgct gccacaagcg ccacggcggg aaacgagcga
      481 attgcattaa acatgagccg atactccata ctgttcgcgg taacggccga ctgcagcgag
      541 atactgttcc tgctgcgggt cggccctgag atacgccatc aggtcgtcca gggtggcgat
      601 gctgatcacc ggaatattat agcttgcgcg cacttcctgc acggccgaca gcgcgccgct
      661 gccgcgctcc atgcggtcca gcgcgattgc caccccgcag ggtatagcgt tcgagcccct
      721 tatcaggttg acagactcgc gcaccgaggt gccggccgag atcacgtcgt caacgatgag
      781 caccctgccc gataacggtg cgccgatcac ggcaccgccc tcgccgtggt ccttcgtttc
      841 cttgcgattg aagctgtaac gcacgttgcg cccggcatcc gccagtgcga tcgacaccgc
      901 cgccaccagg ggtatgccct tgtaggccgg cccgaacagc atgtcgaacc cggtgtccgc
      961 cgcgaggatc gctttcgcgt agaattgcgc cagctgtttc agcgcttctc cgtcgtagaa
     1021 caggccggcg ttgaaaaaat acggcgagag acgccccgcc ttggtcttga attcgccgaa
     1081 gcgcaggacg ttctgccgga tcgtgaagcg gatgaactct tgcctgaagt cggaggtcat
     1141 ggcgcggtgc gggttttcgt tttggcgaat tgaccggtgc gcgttcagcg catccggtcg
     1201 aatttgccgg gagcacgatt tcgctggtca gaacccgtga gaattataac gcttaacctc
     1261 aacggcatcc gctccgcggc gcgcaagggg gtattccggt ggatggcgcg gacccgggcg
     1321 gacgtggtgt gcgtgcagga gctgaaagcg caggcggccg acctcgaaga ggagatgcgc
     1381 gcgccgggcg gcttccacgg cttttttcac catgccgaga agaagggcta cagcggggtg
     1441 gggttgtaca gccgccgcga gcccgatcgc gtggtgcagg gcatcggcga taacgaggcg
     1501 gactgtgaag gtcgctacgt gcgggccgat ttcgggaaac tttccgtcgt ctccgtctac
     1561 ctgccgtccg gctcggccgg cgagcaccgc caggcagcca agttcaggtt catggagtgc
     1621 ttcttcccct ttctgaagcg cctcgcgtct gaaggccgcg agatccttct gtgcggcgac
     1681 tggaacatcg cgcacaggga aatcgacctc aagaactggc gctccaaccg gaagaactcg
     1741 ggtttcctgc cggaggagcg cgcctggctc acccgggtat tcgacgagct gggctgggtc
     1801 gacgtctatc ggcggctgta tccggatgcc acgggcgaag cctatacgtg gtggagcaac
     1861 cgcgggcagg cttacgcgaa caacgtggga tggcgcatcg attaccacat cgccacgccc
     1921 gggctcgcgg cggccgcgaa acgggcggcc atctacaaga cgcggcgctt ctccgaccat
     1981 gcgccgctca ccatcgatta cgatttcggg tcctgatggc cgggtcaccc atccccgccg
     2041 catccgcaag accgccctgg cgcgacacgc tggccgcgat cttcagcggc cgcatgctgt
     2101 acgcgctgct gatgggcttc gcgtccgggc tgccgctgct gctcacgggt tcggtgctgc
     2161 aggcgtggtt gaagaggagc ggcatcgatc tcaccagcat cggcctgttt gccctcgtcg
     2221 gcctgccgta cacgctgaag ttcctgtggg cgccgctgtt cgaccgctac ctgccgccgc
     2281 tcttcgggcg ccgccgcggc tggctgctgg caatacaggc gctgctggcg gcggcgctgt
     2341 tcgcgctgag ttttgcgcat cccgctccgg acggcctgct ggcggtgtcg gtcgccgcgc
     2401 tgctggtcgc gttcttctcg gcatcgcagg atatcgtgat cgacgcctac cggcgcgaaa
     2461 gtctcgccga cgtcgagctc gggttcggat ccgccatgta cgtcaatggt taccggatcg
     2521 gcttgctcgt cgccggcgcc ggcggcctga tactcgccgg cagctattcg tacgagacga
     2581 tgtactgcgt gatggccgcc gtcatggcgg cctgcattgc ggtgacgctc gcggcgccgg
     2641 agccgccgct gcccgcgggc aggcccgtga cgctggcgga ggcggtgttc ctgccgttcc
     2701 gcgattactt cacgcgcggc ggcgcgtggc tcgcgctcgc tttcatcctg ctctacaagc
     2761 tgggcgatac catggcttcg gccatgacga cgccgttcta cctcgaccgc ggctacagcg
     2821 aaaccgagat cggcaccgtg gtgaagctgt tcggtttctg ggctacgatc gccggcggca
     2881 cgctcggcgg cgtctggatc ctgcgcatcg ggatgtcgcg cgcgctgtgg ctgttcggcc
     2941 tggggcaaat gctatcgacg ctggggttcg tggtgctggc gggcgtcgca cccacggccg
     3001 cggcgctggc gacggtggtt gccatcgaga atttcaccgc cgggctgggc acggcggcgt
     3061 tcgtcggctt catggcttcc ctcaccgacc ggcgcttcac ggcgacgcag tacgcgctgc
     3121 tctccagcct gatgggcgtg ccgcgcgtgt tcgcttccgc gccgaccggc tggttcgccg
     3181 aattgatggg ctggcccgga ttcttcctga tgtgcacgtt gatcgcgatt cccggactgc
     3241 tgctgctgcg ctggattgcg cggctggccg acggcgcggg ttcgtccagg tgatggacgg
     3301 cgggtgggcc gagcgacgcg tccgtggtag aagtcgaaat gttggccggg gaggagtgat
     3361 atggcgcaaa cggaaccgct gagcgttcac acgggtggaa cggaaatacc cgttccgcgc
     3421 gatttccgct ggcccggcgg caagcgcatc gcggtgatct tccgcgtggc attcgaggcc
     3481 tggtccgacg accactggcc cggcatcagt cccatgggca acccgctgaa gcccggcttt
     3541 cccgacttga atgcgcgcgg ctgggcggat tacgcctacc ggcgcggcgt gttccgcgcg
     3601 cttgacgcgc tcgcccatca ccgcgtcaag gcaacattca tggtatgcgg gatactggcg
     3661 gagcgctttc cgcacatcgt gaagcaaatc gccgacgccg ggcacgaggt ggccgcgcat
     3721 tcgtacacca tgaacatgat gtcggtgtac atgagcgagg aggaggagcg cgcgaatatc
     3781 cgccacaacg tggaactgat cgagcgcgcc accggcaggc ggccggtggg ctggatcaac
     3841 ccgcgcggca cgccggggcc gaacacggcg cgcctgctgg cggaggaggg gttcgcatgg
     3901 cacggcgaca cgttgaacga cgacctgccg tacgtcgtca agttcggctc ccggtccatc
     3961 gtcgccgtgc ccagcaacat ggacgtcaat gacctgccgc tttacatgaa gcacggccag
     4021 ccgccgggcg tgttcgtgga gaatttcgag ggcttccttc gctacgccca gaagcgcgag
     4081 aagggcgcct cgaagatcga ccccggcatt cactgccacg tttttggccg gccggcgggc
     4141 atgtgggcgt tcgagcgcgt gctggaaatc gcgaagaagt cgaaaggcgt gtggatcggc
     4201 acgcgcgcgg aagcggcgac gcacgtccgc aaggtcctta aaaccccaaa ttagccgcca
     4261 agcctgccct gagtgagtcg aagggacagg ccgccaagaa aaacaaatta gtattcgaac
     4321 tgtcccgacg ccaggaaatt caagattgaa ggctagtgtc ctgagtccct agttcgctga
     4381 acaaaagatg ccgacaattg gcgccatact ttgtcgcaaa tcctcgccag gtgtccaacc
     4441 tggctgtggt ttgcttcgcg tctgacacca atttcggcac ttttgtgatg cacaaggcgt
     4501 actcgagacc aaggatttat caacgaacta gagactcagg acactaaagg agaatgaaat
     4561 gaggctggtg acttttgtgc cgaaaaaggg cgccgcgccg cgcttgggcg tggtgcgcga
     4621 cggcagccag gtggtggatc tgacgccggg cagtggcaag gcgccgtttg acccgtgcga
     4681 catgatttcg ctgattgcct cgggagcgaa gggcctcgcg tgggcgaagc aggccgcggc
     4741 cagggtgaag aagacgctgc cgctcgcgaa agtgaggctg ctggcgccga tcccgcgccc
     4801 gcgcaagaac gtgttctgcg tcggctggaa ctacgtcgag cacttcgagg agggcaagaa
     4861 ggcgctgaag catacccagg agctgccggc gcacccggcc tttttctcca aggtgccgac
     4921 cacggtgatc ggccctcatg accgcgttcc cttcgacccg gccgtgaaca agacgctcga
     4981 ctgggaagcc gagctggggg tcatcatcgg gcgcggcggc aggaacatcg ccgaggccga
     5041 tgcgatgaag cacgtattcg gctataccgt catcaacgac gtgtccgttc gttacatcca
     5101 gaggcagcat ggcgggcagt ggttcaaggg caagagcgtg gacggcacct gcccgatggg
     5161 gccatggatc gtcaccgcgg acgaagtgcc cgatccccac gccctgcgga tcggctgccg
     5221 cgtcaacggc aacaccaagc aggactcgaa cacgagctat atgtatttca aggtcccccg
     5281 gatcatcgcc gagctctcct acggcctcac gctggagccg ggcgacatta tttccactgg
     5341 cacgccggcg ggtgtggggc acgcgcgcac accgccggaa ttcatgaccg cgggtgacgt
     5401 gctggaaacc gaggtagagg gtcttgggtt gcttcgcaac aagatcggaa attagccgcc
     5461 aagcctgccc tgagtgagtc gaagagacgc caaggccgcc aagaaaacct attcaattac
     5521 cagttcacga accggcatat ggggaagcgg aggcctgact tgattcgtag ggtgactggg
     5581 cggctctgtt tgattggatg ttcctggcgg ccttggcgtc ttggcggtta atcggcttca
     5641 cgcccgctgg aagcggtaca ccgccagggc gcccagcagg ttgggcagca cggcgaccga
     5701 ccgggcgttc gtgagcacct tgcgctccag gattctcacg ccgtggcccg cgcagaaatc
     5761 ctcgaagtcg gaaatcgtgc acaggtgcac gttcggcgtg tcgtgccact ggtacggcag
     5821 gttgtccgac accggcatgt ggccggcgag tacctgcagc cggttcttcc agtagccgaa
     5881 attggggaag gtgacgatgc cctcgcgccc gacgcgcagc atgtcgttga tgatgcgctc
     5941 gctgtttttc atggcctgga gcgtctgcga caggaccacg taatcgaacg agccgctctc
     6001 gaattcggag agcccgcgct cgaggtcgct ctggatcacg tcgacgccgt tcgtcacgca
     6061 gcccagcacg ctggcgtcat cgatttccac gccgtagccg gtcacggcgc gggcgccctt
     6121 gaggtacttg agcagcacgc cgtcgccgca gcccaggtcg agcacccgcg agccggggcg
     6181 tatcgactcg gcgatggcgt cgaagtcggc gcgcttgaag ccgttgccgt tcgttctcat
     6241 atgagcaggg gggccgggcc ccttccggta accgcataca gggcctttcc ggccgccccg
     6301 caccctccac cgatggttga aacgggatgt ctcatgcggc gggccgcctc cgatccgttt
     6361 tggagaatat gtgccattca ggtgtcggcg gcagccgcat tcgccctggt cgcatccccg
     6421 gaaaccgggg cccctgcttc cgggctcagc ggtctctccc ttccgatgcg ctcgaagtag
     6481 gctgcgacca ggttgtggta gcgggcgtcc tcgagcagga atgcgtcgtg cccgtgcggg
     6541 gcgttgatct cggcgtaact cacgccgcag cggttgtcga gcagcgcctt gacgatctcg
     6601 cgcgagcgct ggggcgagaa gcgccagtcg gtcgtgaacg aaaccaccag gaaattcgcc
     6661 ctggccgggc tgagcgccct ggcgaggttg ccgccgtgct ccagcgcagg gtcgaagtag
     6721 tcgagcgcct tggtgatgcg caggtacgtg ttggcgtcga agtactccga aaacttgccg
     6781 ccctggtggc gcaggtatga ctcgatctcg aactcggtgt cgaacgtgta gttgagcttg
     6841 ccgttcttca attgccggcc gaacttgctc gccatctcgt cgtccgacag gtaagtgatg
     6901 tgcccgacca tgcgcgcgac gcgcaggccc cgcgtcggcc gggtgttgtg cccgtagtag
     6961 tgcccgccgt gaaaatcggg gtcggtgatg atcgcctggc gcgcgacctc gttgaacgcg
     7021 atgttctgcg ccgacaggtt gggcgcgcac gcgatcacca gcgcgtggcg cacgcgcccg
     7081 ggatggaggt aggtccaggc gagcgcctgc attgcgccga ggctgccgcc catcaccgcc
     7141 gcgaagcggc ggatgccgag atgatcggcc agccgcgcct gggtcgcgac ccagtcctcc
     7201 accgttacca ccgggaaatc cgcgccccac ggcttgcccg ttttcggatt gacgctcatc
     7261 ggcccggtcg agccatggca gccgccgaga ttgttgacgc cgatgacgaa gaagcggctg
     7321 gtgtccaccg gcttgccggg gccgatcatg ttgtcccacc agccgatgtt gtcgggggcg
     7381 tcggcgtaat ggccggcgac gtggtgggcg gcgttcaggg catgacacac cagcaccgcg
     7441 ttggagcggt cggcgttgag cttgccgtag gtctcgtaga ccagctcgta cgcgtccatc
     7501 accgccccgc ttctcaggcg cagcggctcg gtgaatctcg cggtctgcgg cgtgacgacg
     7561 ccgaccgatt tcgatgcaat gcccgtatcc atgaattcac ggcatccaaa aagcgaaaac
     7621 ccggcgagct tgcgcgggcc gggttgggaa aaaacatttt cctctttagc cggatttgta
     7681 acgcgcccgc aagctgaaaa tcaaatcggc gcggctcaaa gcgggaggaa cattaacccg
     7741 caggagacct cttgtcaaat cagctccttg cgagcccgaa acggcgtgaa tcgtgattcg
     7801 cgagtcgcga ttcgtccctg gctcctgcgt cctctgcctc ccgcggggcg agggttcagc
     7861 tattcagttt ttcgagcagc gcccggagcc tggccgggtc ggtggcgcgc agcatcttgc
     7921 gcgcgagcgg ttgcagttcg gcaaaactcg agttgaggat cacctgcttg acgtcgagca
     7981 ggtgcgcgat gtgcatcgag tactgccgca gcccgaacgc cagcagcagc cgagtcagcg
     8041 tggtgtcccc ggccatttcg ccgcacagcg cgaccgggat ctttgccctg tccgcggccc
     8101 tgatgatgcc ggcgatgagg ttcagcaccg ccggatgcag cggatcgtag agatgggaaa
     8161 ccgtatcgtc ggtgcggtcg accgccagcg tgtactggat gaggtcgttg gtgccgatcg
     8221 agaggaaatc gagcttgcgg atgaacatgc cgagggcgag cgccgcggcc ggcacctcga
     8281 tcatgccgcc caccttgacg ccgtgatcgt agggcacgcc ttcctcgtcg aggccgtgct
     8341 tcgcccgcct gatcaacgca agcgtctggt cgatctcggc gaaggtggag agcatcggca
     8401 ccagtatgtt gatcctgccg tggcgcgagg cgcgcagcag ggcgcgcaat tgggtgacga
     8461 accgctgcgg ctcggtcagg cacaggcgga tggcgcgcag tccgagcgcc gggttggtgg
     8521 tgacgcgctc gttttcgtcg tccttgtctc cgccgagatc gtaggtgcgg atggtgaccg
     8581 gcaggccttc catctcctcg gcaaccttgc ggtaggcctc gaactgctcg tcctcgtccg
     8641 gcaggtcggc gcggttcagg aacaggaatt cgctgcggaa gagcccgatg ccggttgccc
     8701 cgttgccgcg ggcgtcggcg acgtccgccg gcagctcgat attggcctgg agttcgacgg
     8761 gcacgccatc gcgcgtgacc gcgcgccgat ccttgagatg cctcaatttc tggcgctcga
     8821 ggtcgaactg ctgctgccgc tcctggtact cggcgagcac ctgctggtcg gggtccacga
     8881 ttaccacgcc ctgggagccg tcgacgatga tgacctcgtt ctcgcggatc atctgccgtg
     8941 cccggtgcag cgccacgatc gacgggatgg cgaggctgcg cgcgaccacc gcggtatgcg
     9001 aggtggtgcc gccgagatcg gtgatgaagc tcgcgtactg gtgccgcttg aactggacca
     9061 cgtcggcggg cgagaggtcg tgcgcgacca ggatcacgtt cggcccggtc tcgggccgcg
     9121 gcggcgggac atagccgggc tggccggaca gcgccttcat cacgcgctcg gcgacctgga
     9181 tcacgtcggc cctgcgctcg cgcaggtagt cgtcctcgat ctcgtcgaac tgggcaagca
     9241 gcgcgtcggt ctgcaccttg agcgcccact cggcattgca tccctcggtc tcgatgatcc
     9301 tgcgcggcgc gaccgacagc gtggaatcgt tgaggatcat caggtgcagg ttgatgaaag
     9361 cagcgaactc cgcgggggcg gtgctcggca cgctggcgtg cagttcctcg aactccgcgc
     9421 gcacctgcct gaccgccgca tcgaaacgcg aggattcctc cggcgcctgg tgcggcggaa
     9481 cgagatagcg ctgcacctcg aggccggtgt gcgtcatcag ctgcgcttgc ccgatggcga
//
LOCUS       DBNGSG010000119         3006 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2927791, whole genome shotgun sequence.
ACCESSION   DBNGSG010000119 DBNGSG010000000
VERSION     DBNGSG010000119.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2927791"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="AC158Q_03055"
     CDS             <1..353
                     /locus_tag="AC158Q_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HIR3338776.1"
                     /translation="VVVGSNLFGDILYDLGPAATGTIGIAPSANMNPEKKFPSLFEPV
                     HGSAPDIYGKKIANPIGQIWAGAMMLDHLGCPDAGAAVLKAIEKVLQEGPKTPDLGGR
                     AGTADLGKAVAAAL"
     gene            complement(516..971)
                     /locus_tag="AC158Q_03060"
     CDS             complement(516..971)
                     /locus_tag="AC158Q_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338777.1"
                     /translation="MTRIRFLATLALALSLPAGAAEPEISAALTVMGYGPKVKVLVNG
                     ADTGITGGKSQSMRLFNKGHPWSAKASADQRARNFVLVPGENVVTIEFAKDPKTNDRL
                     AIDLDSEGYPAPLLKIVNRGNASGRHVVKLRIEPKAPKDFKPVVISDEK"
     gene            965..1399
                     /locus_tag="AC158Q_03065"
     CDS             965..1399
                     /locus_tag="AC158Q_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338778.1"
                     /translation="MSLEAPPAGNHVTNSATIKGTTKSFTAEDAKKGVLRDRKDAEGA
                     KEDGTTSRQGRKGREEINKKKGAETAPRSFLGTSPEGKTFRKPCSGNATLGAFPSRPS
                     GFAVDLIFISFAIRSASLASFVSRSTFSFAPSASFVTRRIVQ"
     gene            complement(1393..2961)
                     /locus_tag="AC158Q_03070"
     CDS             complement(1393..2961)
                     /locus_tag="AC158Q_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007731265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HIR3338779.1"
                     /translation="MAVAPHALAAQSALDVLREGGNALEAMIAAAATIPVAYPHMNSI
                     GGDSFWLVRGLRGAPRGIDACGAAARAASIEWYAARGMAQAIPFRGGVAANTVAGTLS
                     GWDAAFRISRTWGGRLPLKRLLADAIHYAERGVAVTASQSSATAAKRSELESQPGFRE
                     TFLPGGAVPPAGSRFRQPRLAHTLARLARAGLADFYAGKLAKAIARDLATVGSPLAAE
                     DLRRHRARLVTPLALKHSLGTVYNLPPPTQGLVSLLILGMLDALEVGMAGPASADYVH
                     LAVESTKRAFAVRDHCLTDPAYMTVDPQALLAPGKIRELAANVDRGRAARWGAGRPPA
                     DTVWLGVVDAAGRSVSMIQSIYHEFGSGVVLEESGINWQNRGCGFSLDPKALNSLKPG
                     RKPFHTLNPALAVLNDGRVMVYGTMGGDGQPQTQAAVFTRIAVFGMDPQQAVSAPRWL
                     LGRTWGQSSDSLKLESRFSTGVVADLARRGHEMEVLGEHDEAMGHAGAIVRHGNGVLE
                     GGADPRSDGCVAAY"
BASE COUNT          517 a         1006 c          981 g          502 t
ORIGIN      
        1 acgtcgtggt aggctccaac ctgttcggcg acattctcta cgacctgggg cccgcggcga
       61 ccggcaccat cggcatcgcg ccctcggcca acatgaatcc cgagaagaag ttcccgtcgc
      121 tgttcgagcc ggtgcacggc tcggcgccgg acatctacgg gaagaagatc gccaacccca
      181 tcggccagat ctgggccggc gcgatgatgc tcgaccacct cggctgcccg gacgcgggcg
      241 cggcggtgct gaaggcgatc gagaaagtcc tgcaggaagg ccccaagacg ccggacctgg
      301 gtggcagggc cggcaccgcc gatctgggca aggccgtcgc cgcggcgctt taacagacag
      361 gattaacggg gatacattga aaacctgtta accccgtaaa tggacaggat taacaagatt
      421 aacaggattc aaacaacggc tttttcgttt cgtgaatctt taaatcttgt aaatcctgtt
      481 aatcctgtct atttttggtt ttgacgggtt tagggtcatt tctcgtcgga aatgaccacg
      541 ggcttgaaat ccttcggggc cttcggttcg attctcagct tcacgacgtg cctgccggag
      601 gcgttgcccc ggttgacgat tttcagcagc ggcgcgggat agccttcgga atccaggtct
      661 atcgccaggc ggtcgttcgt cttcgggtcc ttggcgaatt cgatcgtcac cacgttttcg
      721 cccggcacca gcacgaagtt gcgcgcccgc tggtcggcag aggccttcgc cgaccacggg
      781 tgccccttgt tgaaaagccg catgctctgc gacttgccgc cggtgatccc ggtatcggcg
      841 ccgttgacga gcaccttcac cttggggccg tagcccatta ccgtgagcgc cgccgagatc
      901 tccggctccg ccgcgccggc gggcagcgac agggcgaggg cgagcgtggc aaggaatcgt
      961 atgcgtgtca ttggaagccc ctcccgccgg aaatcatgtc acaaacagcg cgacgataaa
     1021 aggcaccacg aaaagcttca ccgcggagga cgccaagaaa ggcgtactac gggacaggaa
     1081 ggacgcggag ggcgccaagg aagacggcac gacgagtcgc caaggacgca aaggccgaga
     1141 agaaatcaat aaaaaaaagg gcgcggagac agcgccaagg tcgtttttgg gcacgtcgcc
     1201 ggagggcaag accttccgaa aaccttgcag cggaaatgcc acgcttggcg cgtttccttc
     1261 gcgtccttca ggctttgccg ttgaccttat ttttatttcc ttcgcgatcc gtagtgcctc
     1321 cttggcgtcc ttcgtgtccc gcagtacgtt ttcctttgcg ccctccgcgt ccttcgtgac
     1381 ccgtcgtata gttcaatagg ctgcaacgca gccgtccgag cgcgggtccg cgccgccttc
     1441 gagaacgccg ttcccatggc gcacgatggc gccggcatgc cccatcgcct cgtcgtgctc
     1501 tcccagcact tccatctcgt gcccgcggcg cgcgagatcg gcgacgaccc cggtggaaaa
     1561 gcgcgattcg agcttcagtg aatcgctgct ctggccccag gtgcggccga gcagccagcg
     1621 cggggcggag accgcctgct gcgggtccat gccgaacacc gcgatgcgcg tgaacaccgc
     1681 cgcctgcgtc tgcggctggc cgtcgccgcc catcgtgccg taaaccatca cgcggccgtc
     1741 gttcagcacc gcgagcgcgg gattcagcgt gtgaaacggc ttcctgccgg gcttcaggct
     1801 gttcagcgcc ttggggtcga gcgagaagcc gcagccgcgg ttctgccagt tgatgccgga
     1861 ctcctccagc accacgccgc tgccgaactc gtggtagatg ctctggatca tggaaaccga
     1921 gcgcccggcc gcatccacca cgcccagcca gacggtatcc gcgggcggcc ggcccgcacc
     1981 ccagcgcgcc gcacgcccgc ggtcgacgtt cgccgcgagc tcgcggatct tgccgggcgc
     2041 gagcagcgcc tgcgggtcaa ccgtcatgta ggcggggtcg gtcaggcagt ggtcgcgcac
     2101 ggcgaatgca cgcttggtcg attccaccgc gaggtgcacg tagtccgcgc tcgccggacc
     2161 cgccatgccc acctcgagcg cgtccagcat gccgaggatg aggagcgaga ccaggccctg
     2221 cgtcggcggc ggcaggttgt agaccgtgcc gagcgagtgc ttcaacgcga gcggcgtcac
     2281 cagccgcgcc cggtggcgcc tgagatcttc cgcggcgagc gggctgccca ccgtggcgag
     2341 gtcacgcgcg atcgccttcg ccagcttgcc cgcataaaaa tccgcgagcc ccgcgcgggc
     2401 aaggcgtgcg agcgtgtgcg cgaggcgcgg ctggcggaac cggcttcccg ccggcggcac
     2461 cgcgccgccg ggaagaaacg tttcccggaa accgggctgg gattcgagct cgctgcgctt
     2521 ggccgcggtc gcggacgatt ggctcgcggt gaccgcgacg ccccgctccg cataatgaat
     2581 cgcgtcggcc agcaatcgct tcaacggcag ccgcccgccc cacgttcgcg atattctgaa
     2641 agcggcatcc cagcccgaca gggtgccggc gacggtgttg gcggcaacgc cgccgcgaaa
     2701 cgggatcgcc tgcgccattc cgcgcgcggc gtaccactcg atggatgccg cgcgcgccgc
     2761 ggcgccacag gcgtcgatgc cgcgcggtgc gccgcgcagc ccgcgcacga gccagaacga
     2821 gtctccgccg atcgagttca tgtgcgggta cgcgacgggg atcgtcgcgg ccgcggcgat
     2881 catcgcttcc agcgcgttgc cgccctcgcg cagcacgtcg agtgccgact gcgcggcaag
     2941 cgcatgcggc gcgaccgcca tgccgcgcgt gccgtagagg gaacggatcg tcatggttgg
     3001 atggcc
//
LOCUS       DBNGSG010000120         3167 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3437002, whole genome shotgun sequence.
ACCESSION   DBNGSG010000120 DBNGSG010000000
VERSION     DBNGSG010000120.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3437002"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="AC158Q_03075"
     CDS             complement(<1..201)
                     /locus_tag="AC158Q_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338780.1"
                     /translation="MSNEPIEVKALLGDYPNTRALKQGEVRSPRLAFDFAEVKTPNHA
                     FKRVVRELEFDVAELAIGTYLTA"
     gene            complement(337..1191)
                     /locus_tag="AC158Q_03080"
     CDS             complement(337..1191)
                     /locus_tag="AC158Q_03080"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3338781.1"
                     /translation="MATKVADIHPHIISSDTKRYPLAPLGGEQSGWSQKRPVSFEQLL
                     AAMDRAGIGKAAIVHASTVYGFDNSYVADAVAARPDRFTGVFSVDVLAPDAPAKIRHW
                     VGRRLTGLRLFTTGSTMPGQQPWLEDPRLIPAWECAGEMNIPICMQMTPAAIPQLEKL
                     LKRYPKIRVILDHLLKPGIEDGPPYPKAQFLFDLARYGNVYLKCTSRNVEEARKGKAT
                     PETFFVRLVKEFGANRIAWGSNYPASEGTLEQFVTEVKQALASLPQQDQDWILHKTAQ
                     TLYPALAD"
     gene            complement(1324..2307)
                     /locus_tag="AC158Q_03085"
     CDS             complement(1324..2307)
                     /locus_tag="AC158Q_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3338782.1"
                     /translation="MPEAALPLKIAIATYGHTAALKRGEVPIAGVKPDFIEVKPIIAA
                     FRRMVRDVEFDVCEMAPATYMIAREHGSPFKALPVFIYRNFHHRGFVYRDDAGIRGPK
                     DLEGRKAGVRAYSVTTGIWTRGILQNEYGVDLSRVTWVVDDEEHVKTLKLPPNVVHAP
                     AGRSLVDMMAAGEIQAAFTDNAGIGRAGAPKDGWQATAQRPPSYVDLFPDPPRLEAEW
                     FRKTGIYPVHGLIVVKDELLAREPWVAKALFDAFLESRERYLEQIKAGASVSDKDDFY
                     RQMGAIVGDPLPYGSKANRPAIEALITYCHQQGLLKKRYAVEDMFLDTENS"
     gene            complement(2432..>3167)
                     /locus_tag="AC158Q_03090"
     CDS             complement(2432..>3167)
                     /locus_tag="AC158Q_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR3338783.1"
                     /translation="VAELAIVTYLMARAHGKPYTLLPVVVLSRFQHPFLVYNAARGEL
                     RPGDLKGKRVGIRSYSVTTTMWLRGILMNDHGLDIDSVQWVTTEEAHVAEFRDPPNVR
                     RAPAGRDLLSMLFDGELDAMVLADKTLKDPRLKRLIPEPEKAAQDWHARNRAIQINHM
                     VVVRDSLTKSSPDAVREVYRLLKEARRAAPPPAPGELDMTPIGLDIMRRDLDIAIEYV
                     YQQRLIPRRFKVDELFDDVTRKLDAS"
BASE COUNT          503 a         1007 c         1047 g          610 t
ORIGIN      
        1 ggcggtgagg taggtgccga tggcgagctc cgccacgtcg aactcgagtt cgcgcaccac
       61 gcgcttgaac gcgtggttgg gcgtcttcac ctcggcaaaa tcgaacgcga gccgaggaga
      121 gcgcacctcg ccctgcttca gcgcgcgcgt gttggggtaa tcgcccagca gcgcctttac
      181 ttcaatgggt tcgttactca tatgttgtct cctgaccagg tcttggggta atgaagaaac
      241 ccgcaggcgg aattcaaaag tcggaagtcg aaagtaacgg cagggcggcg cgttgtttgg
      301 cctccgcctt tcgattttcg ccttccgacc tgccgttcag tccgccagcg ccgggtaaag
      361 cgtctgcgcc gtcttgtgca ggatccagtc ctggtcctgc tgcggcagcg aggcgagcgc
      421 ctgtttcacc tcggtcacga actgctcgag cgtgccttcc gacgcggggt agttcgagcc
      481 ccaggcgatg cgattggcgc cgaactcctt gaccaggcgg acgaagaacg tctccggggt
      541 tgcttttccc ttgcgcgcct cctcgacgtt gcggctggtg cacttcaggt acacgttgcc
      601 gtaacgcgcg aggtcgaaca ggaattgcgc cttgggatac ggcggcccgt cctcgatgcc
      661 gggcttcagc agatggtcga ggatcacgcg gattttggga tagcgcttga gcagcttctc
      721 gagctgcggg attgccgccg gcgtcatctg catgcagatc gggatgttca tctcgccggc
      781 gcattcccat gccggaatca gccgcgggtc ctcgagccag ggctgctgcc ccggcatggt
      841 gctgccggtg gtgaacaggc gcagcccggt gagcctgcgg ccgacccagt ggcggatctt
      901 cgccggcgca tcgggcgcga gcacgtccac cgagaacacg ccggtgaaac ggtcgggccg
      961 cgccgcgacc gcgtcggcca cgtacgaatt gtcgaagccg tagacggtgg aggcgtgcac
     1021 gatggccgcc ttgccgatgc ccgcgcggtc catcgcggcg agcaactgct cgaagctgac
     1081 cggccgcttc tgcgaccacc ccgactgctc gccgcccagc ggcgccagcg ggtagcgctt
     1141 cgtgtcgctc gaaatgatat gcggatggat gtccgccact tttgtcgcca tttcaacttc
     1201 cttcctggag ggtgggtcgg tatttcaaag ccgaaggcct gggagcggct cgtgaaacgg
     1261 gaaacatgaa acgcgcgggg attctccgtt tttccattca cgattttcga ttcacggagg
     1321 ccgtcatgaa ttctccgtgt cgagaaacat gtcctccacc gcgtagcgct tcttgagcaa
     1381 tccctgctgg tggcagtagg tgatgagcgc ttcgatcgcg gggcggttgg ccttgctgcc
     1441 gtagggcagc gggtcgccga cgatggcgcc catctgccgg tagaaatcgt ccttgtccga
     1501 gacggatgcg ccggccttga tctgctcgag gtagcgctcc ctggactcga ggaaggcgtc
     1561 gaacagcgcc ttcgccaccc acggctcgcg ggcgagcagt tcgtccttca ccacgatcag
     1621 cccgtgcacg gggtagatcc cggtcttgcg gaaccattcc gcctccagcc gcggcgggtc
     1681 cgggaacagg tcgacgtaac tgggcgggcg ctgcgcggtc gcctgccagc cgtccttggg
     1741 cgcgccggcg cggccgatgc cggcgttatc ggtgaacgcc gcctggatct cgcccgcggc
     1801 catcatgtcg accagcgagc gccccgccgg cgcgtgcacg acgttcggcg gcagcttgag
     1861 ggtcttcacg tgctcctcgt cgtccaccac ccaggtgacc ctcgagaggt ccacgccgta
     1921 ctcgttctgc aggatgccgc gcgtccagat tccggtcgtc accgagtacg cgcgcacgcc
     1981 cgctttcctg ccctcgaggt ctttcggacc gcggatgccg gcgtcgtcgc ggtagacaaa
     2041 tccgcggtga tggaaattgc ggtagatgaa caccggcagc gccttgaacg gcgagccgtg
     2101 ctcccgggcg atcatgtagg tcgcgggcgc catctcgcac acgtcgaact ccacgtcgcg
     2161 caccatgcgc cggaacgcgg cgatgatggg cttgacctcg atgaagtcgg gcttcacccc
     2221 cgcgatgggc acctcgcccc gcttgagggc ggcggtgtgg ccgtaagtgg cgatcgcgat
     2281 tttcagcggc agtgcggctt ccggcatcgg cacttcgttc gataaacagg gctgtgagta
     2341 taaccaattc gttgcaggtc gaaagccgga ggcggaaagc aacacccgct ggaaaaagcc
     2401 gttttcgact ttcgcctttc gcctatcgac ttcaggaagc gtcgagcttc ctcgtgacgt
     2461 cgtcgaacag ttcgtccacc ttgaagcgcc tggggatgag ccgctgctgg taaacgtatt
     2521 cgatcgcgat atcgaggtcg cggcgcatga tgtccagccc gatcggcgtc atatcgagct
     2581 cgccgggggc gggcgggggc gccgcgcgcc tggcttcctt gagcagccgg tacacctccc
     2641 gcacggcgtc ggggctggat ttcgtgagcg agtccctgac cacgaccatg tggttgatct
     2701 ggatcgcgcg gttgcgggcg tgccagtcct gcgccgcttt ctccggctcc ggaatgaggc
     2761 gcttgagacg ggggtctttc agcgtcttgt ccgcgagcac catggcgtcc agttcgccgt
     2821 caaacagcat ggagagcaga tcgcggcccg ccggggcgcg ccgcacgttg ggcgggtccc
     2881 tgaactcggc cacgtgcgct tcttcggtcg tcacccattg gacgctgtcg atgtcgagcc
     2941 cgtgatcgtt catgaggatc ccgcgcagcc acatcgtcgt cgtcaccgag taggagcgga
     3001 tgccgacacg cttgcctttc aggtcgccgg ggcgcagttc gccgcgcgcc gcgttgtaga
     3061 cgaggaacgg gtgctggaag cgcgagagca ccaccaccgg caaaagcgta tacggcttgc
     3121 cgtgcgctct tgccatcagg taggtcacga tcgccagttc cgcgacg
//
LOCUS       DBNGSG010000121         1752 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2759594, whole genome shotgun sequence.
ACCESSION   DBNGSG010000121 DBNGSG010000000
VERSION     DBNGSG010000121.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2759594"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="AC158Q_03095"
     CDS             <1..531
                     /locus_tag="AC158Q_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIR3338784.1"
                     /translation="LNKPMGYVSAQPEKGYLPAVTLVTAETRFGGDRAPLTFSPAHLR
                     GLAPAGRLDIDSTGLLVLTQDGRIAKQLIGEDSGVEKEYLVRVQGTLDAKGLALLNFG
                     LALDGQALKRAEVGWLNRDQLRFILREGKKRQIRRMCELVGLAVTGLKRVRIGNVKLA
                     DLPDGQWRYLRSDERF"
     gene            731..>1752
                     /locus_tag="AC158Q_03100"
     CDS             731..>1752
                     /locus_tag="AC158Q_03100"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3338785.1"
                     /translation="MYYPGNWIHSFPENFQWSNATLICKGMAPYGAVALGEIDWVVQA
                     LHRRAKEPEAWHQEWCKVADKVAARGDEAARAGNQATAGNYYLRAGQYYYTGERMVPP
                     GELKTSIYRKSLHYSQEGLKRRYPNLEIVDVPYEKTGLAAYFLKSPVARGPAPTVVLF
                     NGLDNCKEMNVLFAGVEFAFRGFHTLAIDGPGQGESLRLRNHHARYDYEAAGTPAYEF
                     VAKRKDVDPKRVAIMAYSAGGYYAPRAAAFEPRYAACVAWGPHYDYHAVWEKRWAAMQ
                     ADHNSVATSHFQLPWVLGTKDMDSAMAKVKQFTLAGVADRIKCPFLILWGTQDKLTPR
                     SVADQL"
BASE COUNT          318 a          589 c          572 g          273 t
ORIGIN      
        1 ctcaacaagc cgatgggcta cgtctcggcg cagcccgaga agggctatct ccccgccgtc
       61 acgctggtca ccgccgaaac ccgcttcggc ggcgaccgcg ctcccctcac cttcagcccc
      121 gcgcatctcc ggggccttgc gcccgccggg cggctcgaca tcgactcgac cgggctgctg
      181 gtgctcacgc aggacggccg catcgccaag cagctgatcg gcgaggactc gggcgtcgag
      241 aaggaatacc tggtgcgcgt gcagggcacg ctggacgcga aaggcctcgc gctgctgaat
      301 ttcggcctcg ccctcgacgg ccaggcgctg aagcgcgcgg aagtcggctg gctcaacagg
      361 gaccagctgc gcttcatcct gcgcgaaggc aagaagcgcc agatccgccg catgtgcgag
      421 ctggtcggcc tcgccgtcac cggtttgaaa cgcgttcgca tcggcaacgt gaagctggcc
      481 gacctccccg acggtcagtg gcggtatttg cggagcgacg agcggttcta actgaaaggc
      541 aaaagtcaca aggcaaaagg caaaaggaaa acccgtcaga tagtgtcttt tgtgccttgt
      601 gccttcggca gcgttccgct tcgtggaact tgcgttgacc cggtgtgacc cctgcgctag
      661 tctcgactca tcccagaaga acgcgagcgg ccggcatggc gcgcgctttt ttcacccctc
      721 cggagcttgc atgtactacc ccggcaactg gatccacagt tttcccgaga atttccaatg
      781 gtcgaacgca acgctgatct gcaagggcat ggcgccctac ggcgcggtcg cgctgggcga
      841 gatcgactgg gtggtgcagg cgctgcaccg gcgcgcaaag gagccggagg cgtggcatca
      901 ggagtggtgc aaagtggccg acaaggtcgc ggccaggggc gatgaggcgg ccagggccgg
      961 caaccaggcg acggcgggca actactacct gcgcgcgggg cagtactact acaccggcga
     1021 gcgcatggtg ccgccgggcg aattgaagac gtccatctac cgcaagtcgc tgcactactc
     1081 ccaggaaggc ctgaagcgcc gctatcccaa tctcgagatc gtggacgtgc cctacgaaaa
     1141 aaccgggctc gcggcgtact tcctcaagtc gccggtggcg aggggccccg cccccaccgt
     1201 cgtgctgttc aacggactgg acaactgcaa ggagatgaac gtgctgttcg ccggcgtgga
     1261 gttcgcgttc cgcggctttc acacgctcgc aatcgacggc ccgggccagg gcgagtcgct
     1321 gcggctgcgc aaccaccacg cgcgctacga ctacgaggcc gccggcacgc cggcctacga
     1381 gttcgtcgcg aagcgcaagg acgttgaccc gaagcgcgtc gccatcatgg cctacagcgc
     1441 cggcggctac tatgcgccgc gcgccgcggc gttcgagccg cgctacgctg cctgcgtcgc
     1501 ctggggcccg cattacgatt accacgcggt gtgggagaag cgctgggccg cgatgcaggc
     1561 agaccacaac agcgtcgcca cctcgcactt ccagctgccg tgggtgctcg gcacgaagga
     1621 catggactcc gccatggcga aggtgaagca gttcaccctc gccggggtcg cggaccggat
     1681 caaatgcccc ttcctgatcc tgtggggcac gcaggacaag ctcaccccgc gctcggtcgc
     1741 cgaccagctc ta
//
LOCUS       DBNGSG010000122        12239 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3438761, whole genome shotgun sequence.
ACCESSION   DBNGSG010000122 DBNGSG010000000
VERSION     DBNGSG010000122.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 12239)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12239)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12239
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3438761"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="AC158Q_03105"
     CDS             complement(<1..791)
                     /locus_tag="AC158Q_03105"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HIR3338786.1"
                     /translation="MKLETGNSKPETLGQWLDYVEQLHPKTIALGLDRVEKVKQALGI
                     APTFPIITVGGTNGKGSVCAMLEAILHCSGYRVGCYTSPHLLRYNERVRVSRAEASDS
                     DLARAFSAVESGRGDVPLTYFEMGTLAALWLFAERRVEVAVLEVGLGGRLDAVNAFNA
                     DCAVITTVDIDHVDYLGADREAIGYEKAGIFRGGRPAVCADPAPPASLVRHAGEIGAQ
                     LVRIGADFGVTLQDKQWQYWGPRGKRNALPHPALRGPSQLGNAAAA"
     gene            complement(855..1721)
                     /gene="accD"
                     /locus_tag="AC158Q_03110"
     CDS             complement(855..1721)
                     /gene="accD"
                     /locus_tag="AC158Q_03110"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HIR3338787.1"
                     /translation="MSWLQKLLPPKIKRDATTPKKVVPEGLWSKCESCEAVLYRADLE
                     KNLHVCPKCSHHNRISARERLDVLLDREGRYEIGAEVAPVDTLKFKDSRRYAERLEEA
                     RSETSEEDALVVMQGSIKTIPLVAAAFEFGFLGGSMGSVVGERFVRGVKVCLEQRLPY
                     ICITASGGARMQEGLLSLMQMAKTCASLTQLSRERLPFLTLLTDPTMGGVSASFAMIG
                     DVVIAEPGALVGFAGPRVIEQTMRETLPEGFQRSEFLLKHGAIDLIVDRRELRDRLAG
                     LLGMLLRLPPAA"
     gene            complement(1718..2533)
                     /gene="trpA"
                     /locus_tag="AC158Q_03115"
     CDS             complement(1718..2533)
                     /gene="trpA"
                     /locus_tag="AC158Q_03115"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HIR3338788.1"
                     /translation="MSRIASTFEALKKHGRKALIPFITAGDPDPRLTVPLMHALASAG
                     CDVIELGVPFSDPMADGPVIQRSSERALKHGVSLRDVLGYVAEFRKSDGSTPVVVFGY
                     ANPIEALGLERFADAAKSAGADGVLVVDYPPEEARPLVRLLDARGLDTIFLLSPTTTD
                     IRLREVGELGRGYLYYVSLRGVTGAAHIDLADVAARVKHVRGFARLPVGVGFGIRDAQ
                     SARAVGTVADAVVIGSALIQEIEKSPRDKVTAQVTRFLAPIREALDSISTVKA"
     gene            complement(2533..3726)
                     /gene="trpB"
                     /locus_tag="AC158Q_03120"
     CDS             complement(2533..3726)
                     /gene="trpB"
                     /locus_tag="AC158Q_03120"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HIR3338789.1"
                     /translation="MVDLPDASGHFGPYGGVFVAETLVQALDELRAAYERYRNDPEFQ
                     AEFAYELKHYVGRPSPVYHARRWSEHFGGAQVYLKREDLNHTGAHKINNVIGQALLAR
                     RMGKRRVIAETGAGMHGVAAATIAARYGMECVVYMGSEDVRRQVQNVYRMKLLGATVV
                     QVESGSKTLKDALNEAMRDWVTHVENTYYIIGTVAGPHPYPVMVRDFQSVIGREAREQ
                     MLELAGRQPDAVLACVGGGSNAMGIFHDYIPDEKVRLIGVEAAGHGLESGKHAATLAA
                     GKPGVLHGNRTYLLQDENGQILSTHSISAGLDYPGVGPEHAWLKDNGRAEYVSVTDEE
                     ALQAFHDLCRLEGIIPALESAHALAYAAKMAPRMKKDQILLVNLSGRGDKDMHTVAEK
                     SGIKF"
     gene            complement(3719..4342)
                     /locus_tag="AC158Q_03125"
     CDS             complement(3719..4342)
                     /locus_tag="AC158Q_03125"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HIR3338790.1"
                     /translation="MATAVKICGITRPEDALAAARAGAHAIGLVFYAGSPRYVTPERA
                     REIIRVLPPFVTAVGLFVDARLEEVRSTLAEAPVGLLQFHGAEAPEFCRQFKRPYVKA
                     VRVKPGVDLLQYARDYHDAKALLLDNYVEGLHGGSGVAFDWSLIPRGLPLPVILSGGL
                     TPENVMDAVRRVRPSAVDVSSGVESAKGIKDAVKIAAFIKGVRNADG"
     gene            complement(4348..5136)
                     /gene="truA"
                     /locus_tag="AC158Q_03130"
     CDS             complement(4348..5136)
                     /gene="truA"
                     /locus_tag="AC158Q_03130"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018439807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIR3338791.1"
                     /translation="MRIALGVEYDGSRFCGWQTQPQGCAVQDALERALTEVASGPVTT
                     VCAGRTDAGVHALAQVVHFDTGAERPEAAWVRGVNALLPSACAVNWSRPVAQDFHARY
                     SALARHYRYLLLNHPVRPAVDRERVGWFHLPLDLEKMQRAARILIGEHDFSAFRSSEC
                     QARTPVRALTRLDIDRRGDYLMFEFSANAFLHHMVRNIVGCLVYVGKGKYPPEWLGEL
                     LAGRDRKHAAPTFEAAGLYLSRVTYDERWGLPQTPRATLMSMAH"
     gene            complement(5228..7690)
                     /locus_tag="AC158Q_03135"
     CDS             complement(5228..7690)
                     /locus_tag="AC158Q_03135"
                     /inference="COORDINATES: protein motif:HMM:NF051932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="motility hub landmark protein FimV"
                     /protein_id="HIR3338792.1"
                     /translation="MRDSMKTWLVALLAALLPCVAGAAGLGRLNVLSSLGQPLIAEIE
                     LVSATREELGSLNARLAHPDAYRLANLQYNPALTGTRLTIERRAGGQHYIKLVSTRPV
                     SEPFIDLLIELTWASGRLSREYTALLDPPGITPAAPAPVISAVPESRPAPEPQPATAP
                     EPVASRPVTPAPAPVAPMAGGKEYGPIERGETLGKIAQSVMPEGVTLEQMLVALYRSN
                     PDAFIRKNLNLVRAGKILRVPDREEVAAIGSGEAVKEYRAHVTDWNSYRQKVAEAAGT
                     VPVEGRTAVSGKITTKVEDKIAGAPKDVVKLSKGEAPGAGKGKPGSPADRVRMLEEEA
                     VAREKALNEANERIAQLEKTLQDMKKLVELKSPGMAAAQQKAQQAATAKPDTMAAAKP
                     EPAKPEPVKPQETPSAIAKPEEKSETMAAPAPKKDVPASKPKAKPAAKAKEPELVDQI
                     MEAATDPLYLGIGGGVLVLGGLAFFLARRRRSRADADEPPGASRLGTAAATAAGAAAI
                     TADATAAPEPAAAGAEDVDPLAEAEVYIAYGRDGQAEEILKDALSKNPKREDAQLKLL
                     EIYSARKDKAAFAKQAGDLNKLTGGSGDNWIKAAAMGYALDSSNPLYAAGKDAVVATA
                     DTAVADVDLDLGMGEAGTSTDITLDAGTAATGTDGAILDLGAGEKAEAQPQPGGMPDF
                     ALEVPAAEGAQSNPAPEPAAPAQDAGGMLDFQIELPKAAEEPATTATPPSATASGADA
                     GLDFKLDGLNLNLDDEPKTAAAAGGEKDGHWYDVQTKFDLAKAYQEMGDKDGAKEILQ
                     EVIKEGDTEQKSQAKTLLDSLG"
     gene            complement(7958..9079)
                     /gene="asd"
                     /locus_tag="AC158Q_03140"
                     /pseudo
     CDS             complement(7958..9079)
                     /gene="asd"
                     /locus_tag="AC158Q_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106601.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
     gene            complement(9181..10245)
                     /gene="leuB"
                     /locus_tag="AC158Q_03145"
     CDS             complement(9181..10245)
                     /gene="leuB"
                     /locus_tag="AC158Q_03145"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HIR3338793.1"
                     /translation="MKIAVLAGDGIGPEIVAQAVKVLEALARDGLKLELEEAPFGGAA
                     VDAHGDPLPESSLKLAKAADAVLCGAVGGPKYDALPRPQRPEQGILRIRRELALFANL
                     RPALMFPELVGASTLKPEVVSGLDVMIIRELTGDIYFGEPRGRRDNSRGEREGYDTML
                     YSEPEIRRIAEVGFATAMKRGRKLCSVDKENVLETSRFWREIVNDVAKKFPQVALSHM
                     YVDNAAMQLVRAPKQFDVIVTGNMFGDILSDEASMLTGSIGMLPSASLDASGKGLYEP
                     SHGSAPDIAGRNIANPLATILSSAMMLRYTFNKDEWAYRIEAAVKSVLAQGFRTADIH
                     EAGTQKIGTREMGDAVVAAL"
     gene            complement(10368..11006)
                     /gene="leuD"
                     /locus_tag="AC158Q_03150"
     CDS             complement(10368..11006)
                     /gene="leuD"
                     /locus_tag="AC158Q_03150"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008164867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HIR3338794.1"
                     /translation="MEKFIRQEGLVAPMDRANVDTDAIIPKQYLKSIKRTGYGPNLFD
                     EWRYLDHGEPGMDHAKRPLNPDFVLNQPRYRGATVLLARENFGCGSSREHAPWALLQY
                     GFQTVIAPSFADIFYNNSLKNGLLLIKLDEMTVDRLFKEAAGTEGYRLAVDLERQTVT
                     TPGGESFKFDIDPFAKHSLLNGLDEIGLTLGHAAEIRAFEAKHRERQPWLFG"
     gene            complement(11017..11142)
                     /locus_tag="AC158Q_03155"
     CDS             complement(11017..11142)
                     /locus_tag="AC158Q_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HIR3338795.1"
                     /translation="MKKPLAFILVALALALAGCNTMHGLGKDVEQLGDKIQKKAQ"
     gene            complement(11154..>12239)
                     /gene="leuC"
                     /locus_tag="AC158Q_03160"
     CDS             complement(11154..>12239)
                     /gene="leuC"
                     /locus_tag="AC158Q_03160"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HIR3338796.1"
                     /translation="IVHVIGPEQGATLPGMTVVCGDSHTSTHGAFACLAFGIGTSEVE
                     HVLATQCLVMKKTRSMQVVVYGRLGRGITAKDVILAIIGKIGSAGGTGYTIEFSGEAI
                     RSLTMEGRMTLCNMSIEAGARAGMVAVDEKTLEYVKGRPMAPKGAMWDRAEAYWRTLI
                     SDAGAKFDEVVTLDATAIQPQVTWGTNPQMVTTIDGKVPDPAQEKDPVKREWIERALK
                     YMNLKPNTPITGIRPDKVFIGACTNSRIEDLRAAAAVVKDKRVASNIKLAIVVPGSGL
                     VKEQAEKEGLDKIFMAAGFEWRQPGCSMCLGMNDDQLSPGERCASTSNRNFEGRQGPG
                     GRTHLVSPAMAAAAAIAGHFVDVRNFK"
BASE COUNT         2050 a         4142 c         3723 g         2324 t
ORIGIN      
        1 gccgcagccg cgttgccgag ctgggacggc ccgcgcagtg ccgggtgcgg gagcgcatta
       61 cgcttgccgc gcggccccca atactgccac tgcttgtcct gcagggtgac gccgaaatct
      121 gcgccgattc tcactaattg ggcgccgatt tcccccgcat gccggacgag gctggcaggc
      181 ggcgcggggt cagcgcacac cgccggccgg ccgccgcgaa aaatacccgc tttctcgtaa
      241 ccgatcgcct cgcggtcggc gcccagataa tccacgtggt cgatgtccac ggtggtaatc
      301 accgcgcagt ccgcattgaa ggcattgacg gcatcgagcc tcccgccgag ccccacttcc
      361 agcaccgcga cctcgacgcg gcgctctgcg aacagccaca gggcggccag tgttcccatt
      421 tcgaaatagg taagcggcac atcgccgcgc ccggactcta cggccgagaa tgcccgcgca
      481 agatcggagt cggacgcctc cgcgcgagaa acacgcacgc gctcgttgta gcgcagcagg
      541 tgcggcgagg tataacagcc gacccggtat cccgaacaat ggagaatcgc ctccagcatc
      601 gcgcacaccg agcccttgcc gttggtgccg ccaacggtga tgatcgggaa tgtcggcgcg
      661 atcccgagcg cctgtttcac cttctcgacg cgatcgaggc cgagcgctat cgtcttcgga
      721 tgaagctgct cgacgtaatc gagccactgc cccagggttt ccggtttcga gtttccggtt
      781 tcgagtttca atgtcgaaag ccaccgcggg cacaatccgc caactgggaa cccgaaactc
      841 gcaactcgaa actgctaggc agcaggtggc agcctgagca gcatcccgag cagaccggcc
      901 agccgatcgc gcaactcgcg gcgatccacg atcaggtcga tcgcgccatg cttcagaagg
      961 aactcggaac gctggaaacc ctccggcagc gtttcgcgca tggtctgttc aatgacgcgc
     1021 ggcccggcaa agccgacgag cgcgccgggt tccgcaatca cgacgtcgcc gatcatggcg
     1081 aagctggccg agaccccgcc catagtggga tcggtcagca acgtgaggaa cggcagccgc
     1141 tcgcgtgaaa gctgggtcag cgaggcacag gtcttggcca tctgcatgag cgaaagcagc
     1201 ccttcctgca tgcgcgcccc gccgctggct gtaatgcaga tgtacggcag gcgctgctcg
     1261 agacagactt tcactccgcg cacgaagcgc tcgcccacaa ccgatcccat cgatcccccg
     1321 aggaacccga attcgaaggc ggccgccacc agcgggatgg tcttgatcga tccctgcatc
     1381 accacaagcg cgtcttcttc cgaggtctcg ctgcgcgcct cctccagccg ttcggcatag
     1441 cgccggctat ccttgaattt gagggtgtcc accggcgcga cttccgcgcc gatctcgtag
     1501 cgcccttcgc ggtcaagcag gacgtcaagc cgctcgcgtg ccgagatgcg gttgtgatgc
     1561 gagcacttgg ggcagacgtg tagatttttc tccagatccg cgcggtagag cactgcttcg
     1621 cacgactcgc acttgctcca cagtccctcc ggcaccactt tcttgggggt ggtggcgtcg
     1681 cgcttgatct tgggcggcag cagtttctgg agccaactca tgctttcacc gtggaaatac
     1741 tgtcaagtgc ctcgcggatt ggggcgagaa accgtgtaac ctgggcggtc accttgtccc
     1801 gcggcgactt ctcgatctcc tgaatcaatg cgctgccgat gacgaccgca tcggcaaccg
     1861 tgccgaccgc gcgcgcgctc tgcgcatcgc ggatgccgaa accgacaccc accggaagcc
     1921 gggcgaaacc gcgcacatgc ttcacgcggg cggcgacatc ggcgaggtcg atatgggcag
     1981 cgccggtcac gcctcgcaac gaaacgtagt agagatatcc ccggccgagt tcccccacct
     2041 ctctcagccg gatgtcggtt gtcgtgggcg agagcaggaa tatcgtatcg agtccacgcg
     2101 catcgagcaa ccgcaccagc ggacgcgctt cctcgggagg atagtccacg accagcacgc
     2161 cgtcggcgcc tgctgacttc gccgcatcgg caaatcgctc cagtcccagc gcctcgatcg
     2221 gattggcata gccaaacacg accaccggcg tggagccgtc ggatttgcgg aactcggcca
     2281 catatcccag cacatccctc agggacacgc catgcttcag cgccctctcg ctggagcgct
     2341 ggatgacggg accgtcggcc atgggatccg agaacggcac accgagttcg attacgtcgc
     2401 accccgccga tgcgagtgcg tgcatcagcg gcacggtgag cctcgggtca ggatcgccgg
     2461 ccgtgatgaa ggggatcagg gccttcctgc cgtgcttctt caatgcctcg aaggtggagg
     2521 cgatccgtga catcagaact tgatacccga tttctcggca acggtatgca tgtccttgtc
     2581 gccgcggccg gagaggttga cgagcaggat ctggtccttc ttcatgcgcg gggccatttt
     2641 ggcggcgtag gcaagcgcgt gcgccgactc gagcgcgggt atgatgcctt ccagccggca
     2701 caggtcgtgg aacgcctgca atgcctcctc atcggtcacc gacacgtact cggcccgccc
     2761 attgtccttc agccaggcgt gctccgggcc gacgccgggg taatcgagac cggccgagat
     2821 cgagtgggtt gagaggatct ggccgttctc gtcctgcagg agataggtgc gattgccgtg
     2881 cagcacgccg ggcttgccgg cggcgagcgt cgccgcatgc ttgccgcttt ccagcccgtg
     2941 ccccgcggcc tccacgccga tcagccggac cttttcatcc gggatgtaat cgtggaagat
     3001 ccccatggcg ttcgatcccc cgccaacgca ggcaagcacc gcgtccggct gccgccccgc
     3061 gagctccagc atctgttcgc gcgcctcgcg cccgatcacc gactggaaat cgcgcaccat
     3121 cactggatag ggatgcgggc cggccacggt gccgatgatg tagtaggtgt tctcgacgtg
     3181 ggtcacccaa tcgcgcatcg cctcgttcaa tgcgtccttg agcgtcttcg agccgctctc
     3241 cacctgcacc accgtggcgc ccagcagctt catgcggtag acgttctgca cctggcgcct
     3301 gacgtcctcg gaccccatgt agaccacgca ttccatgccg tagcgcgccg caatagtggc
     3361 cgccgccacg ccgtgcatgc cggcaccggt ttccgcgatc acgcggcgct tgcccattcg
     3421 ccgcgccagc agcgcctgtc cgatcacgtt gttgatcttg tgtgcgccgg tgtgattcag
     3481 gtcctcgcgc ttgaggtaga cctgcgcgcc gccgaaatgc tccgaccacc gccgcgcgtg
     3541 gtaaaccggg ctggggcggc cgacgtagtg cttcagctcg taggcaaact cggcctggaa
     3601 ttcggggtcg ttccggtagc gttcatacgc ggccctcagc tcgtcgagcg cctgaaccag
     3661 cgtttcggca acgaacaccc cgccatacgg cccgaaatgg ccgctcgcgt cggggagatc
     3721 aaccatccgc attgcgcact cccttgatga acgcggcgat cttcacggcg tccttgatgc
     3781 ccttcgcgga ctcgactccg ctcgaaacat ccactgcact aggccgcacg cggcgcacag
     3841 cgtccatgac gttctccggg gtcaggccgc ctgacagaat gaccggcagc ggcaaaccgc
     3901 gcggaatcag gctccagtcg aatgcgacgc cgctcccgcc atgcaatccc tcgacataat
     3961 tatcgagcag cagcgccttg gcgtcgtgat aatcccgcgc gtattgtagc aaatccaccc
     4021 ccggctttac ccgcaccgct ttgacgtagg gacgcttgaa ctggcggcag aattcgggcg
     4081 cttcggcgcc gtggaactgg agcagcccga ccggcgcttc cgcgagcgtg gagcgcactt
     4141 cctccaaccg ggcgtcgacg aacaacccga ccgccgtgac gaacggcggc agcacgcgga
     4201 taatctctct ggcgcgttcc ggcgtgacgt atcgcgggct tccggcatag aaaacgagac
     4261 cgatcgcgtg cgcgccggcg cgcgccgcgg cgagtgcgtc ctccggacgg gtaatcccgc
     4321 agattttgac ggcggttgcc atgcgcgtta atgggccatg ctcatgagag tggcgcgcgg
     4381 cgtctgcggc agaccccagc gctcatcgta agtcacccgt gacaggtaca ggcccgccgc
     4441 ctcgaatgtc ggtgcggcat gttttcggtc ccggcccgca agcagctcgc ccagccactc
     4501 cggtggatat tttcctttcc cgacgtatac caggcaaccc acgatgttcc gcaccatgtg
     4561 atgcaggaac gcattggcgc tgaattcgaa catcaggtag tcgccgcggc ggtcgatgtc
     4621 gagacgcgtc agcgcgcgca ccggcgtgcg cgcctggcat tccgagcttc ggaacgcgct
     4681 gaaatcatgc tcgccaatca ggatccgtgc tgcgcgctgc atcttctcca ggtcgagcgg
     4741 cagatggaac catccgaccc gctcccggtc cacggccggc cgcaccgggt gattgagcag
     4801 aaggtatcga taatggcgcg cgagcgccga gtagcgggcg tggaagtcct gcgccaccgg
     4861 ccgcgaccag ttcacggcgc atgcggaagg cagcagcgcg ttgaccccgc gtacccacgc
     4921 cgcctccggg cgctctgcgc cggtatcgaa gtgcaccacc tgggcgagcg catgcacacc
     4981 cgcgtccgtg cggcccgcgc agaccgtggt gacgggcccg ctcgccactt cggtcagcgc
     5041 ccgctcgagt gcgtcctgga cagcgcaccc ttgcggctgc gtctgccagc cgcaaaaccg
     5101 gctgccgtcg tattccacgc ccaatgcaat cctcatgtcc gcgcgcctgc cgatattcca
     5161 cagccccctg aaaagcaaaa accgcggcag cttgcactgc cgcggttcga tctgtcgccc
     5221 ggcgtgccta tcccaggctg tccaacaggg ttttggcctg cgacttctgt tcggtgtcgc
     5281 cttccttgat gacttcctgg aggatttcct tggcaccgtc cttgtcgccc atctcctggt
     5341 acgctttcgc cagatcgaac ttggtctgca cgtcgtacca atgtccgtct ttttccccgc
     5401 cggccgcggc tgcggtcttg ggttcgtcat ccaggttcaa gttaaggcca tcgagtttga
     5461 agtcgagtcc cgcatccgcg ccggaagcgg tggctgaagg cggcgttgcg gtcgtggcgg
     5521 gctcttctgc tgccttgggc agctcaatct ggaaatcgag catgccgccg gcatcctggg
     5581 ccggggcagc cggctcaggc gccggattgg attgggcgcc ctccgccgcc ggtacttcca
     5641 gcgcgaaatc gggcatccct cccggctgcg gctgcgcctc cgccttttcg ccggccccca
     5701 gatcgaggat agccccgtcg gtgccggtcg ctgcagttcc cgcgtcaagc gtgatatcgg
     5761 tcgacgtgcc ggcttcgccc ataccaagat ccagatcgac gtcggcgacc gccgtgtcgg
     5821 cggttgcgac cacggcgtcc ttgcccgctg catacagggg attggacgaa tcgagcgcgt
     5881 aacccattgc cgccgccttg atccagttgt ccccgctgcc gccggtcagc ttgttcaggt
     5941 cgccggcctg cttggcgaag gcggctttgt ccttgcgcgc ggagtagatc tcgagcagct
     6001 tcaattgcgc gtcctcgcgc ttgggattct tggacagcgc atcttttaga atttcctcgg
     6061 cctggccgtc gcgcccataa gcgatgtaga cctcggcttc cgccaacgga tcgacatcct
     6121 ctgcgcccgc ggccgcaggc tccggtgcgg cggttgcgtc tgcggtaatg gcggctgccc
     6181 ccgccgcagt ggcagccgcc gtgcccagcc tggaagcgcc cggcggctcg tcggcgtcgg
     6241 cccgcgagcg gcgccggcgc gccaggaaaa atgccaaccc gccgagaacg agaacgccac
     6301 cgccgattcc cagataaaga gggtcggtgg ccgcctccat gatctggtcc accaactccg
     6361 gctccttggc cttggccgcg ggtttcgcct tcggtttgga cgcaggcaca tccttcttgg
     6421 gcgccggcgc ggccattgtt tcagacttct cctcgggctt ggcaatcgcg gagggtgtct
     6481 cctgcggctt gacgggttcc ggtttcgccg gctcgggctt ggctgccgcc atcgtgtccg
     6541 gtttcgccgt tgccgcttgc tgcgcctttt gctgggcggc ggccattccc gggctcttca
     6601 gttcgaccag cttcttcatg tcctgaaggg ttttctcgag ctgggcaata cgctcattgg
     6661 cttcgttcaa cgccttctcg cgcgcaaccg cttcttcttc cagcatgcgg acgcgatccg
     6721 ccggactgcc cggcttgccc ttgcccgctc ccggcgcctc gcccttcgac aacttcacga
     6781 catccttcgg cgcgcccgcg atcttgtcct cgaccttggt agtgatcttg ccactgacgg
     6841 ctgtgcgtcc ctccaccgga accgttcccg cggcctcggc cactttctgg cgatagctgt
     6901 tccaatcggt gacgtgggcg cggtactcct tcaccgcctc gcccgaaccg atagccgcga
     6961 cctcttccct gtcaggcact cgcaggattt ttccggcgcg caccaggttg aggttcttgc
     7021 ggatgaaggc atcgggattg ctgcggtaaa gggcaaccag catctgctcg agcgtaacgc
     7081 cttccggcat tacgctttgc gcgatcttgc cgagtgtctc gccgcgctcg atcggcccat
     7141 attccttccc gcctgccatc ggggcaaccg gcgcgggtgc gggcgtcacg ggcctgctgg
     7201 cgacgggctc aggcgcggtc gccggttgcg gctcgggcgc gggtcgggac tccggcacgg
     7261 ccgaaatgac cggcgccggc gctgccggcg taatgccggg tgggtcgagc agcgcggtgt
     7321 attcgcgcga cagacgcccc gacgcccaag tcagttcgat cagcaggtcg atgaaaggct
     7381 cgctgaccgg tcgcgtggag acgagcttga tgtagtgctg cccgccggcg cgccgctcta
     7441 tggtgaggcg ggtaccggtc agagcggggt tgtactgcag gttggccagc cggtaagcgt
     7501 cgggatgcgc gagccgggcg ttgagcgaac cgagttcctc gcgggtcgcc gataccagct
     7561 cgatctccgc aatgagcggc tgacccagcg acgaaagaac attgagcctc cccaagccgg
     7621 cagcgcctgc tacgcacggc agcagagcgg ccaacagcgc caccagccac gtcttcatgg
     7681 aatcgcgcac aacggcaccc ttatgatttt taggggtaaa caggctaaca tcaatcagtt
     7741 agccatgcaa gctagtattt cctctaagtg ttgcgcctga caggactatt tttattcata
     7801 tttcagcatg atagcccgtc cgccgcccgg gccggccgga tcatgacatt gtaatgtctc
     7861 gcggacacaa cactgccgcc agactcgcgg ctcaggcccc taccgctgcc ttgcgctgga
     7921 ctgtcctcgc accggaacgg gaagataccg ctccggtttc aagcaggatg cgcaacatgc
     7981 gccgcagcgg ctcggccgca ccccacagga gctggtcgcc cacggtaaac gcagcgagat
     8041 actcgccgcc catcgcaagc ttgcgcaagc ggcctaccgg gatgtgcatg cggccggtca
     8101 ccgcggccgg cgtcaactcg cgcatcgagc gttcgcgctc gttcggcacg acgcgcaccc
     8161 actcgttggc cgaggcgatg atgccggtca cttcgtcgag cgggacgttc ttcctcaact
     8221 tgatcgtcag tgcctggctg tggcagcgca tcgcaccgac gcgaacgcag aggctgtcga
     8281 tcggcgtcgc gggagagccg aaggactccc cgcggccgag tatcttgttg ctctcggcgc
     8341 cgcctttcca ttcctcgagc gagacgccgt tgcccatgtc cttgtcgatc cagggtatca
     8401 ggttgcccgc cagcggcaca ccgttgaagt tttccatcgg catcgtgccg tcggtttgct
     8461 tgccgagcac cctgcggtcg atctccagga tcgcggaggc gggatcggcg agcagatccc
     8521 tgacttcggc attgattagt ccgaactgcg tcagcaattc gcgcatgtgc tgcgcgccgc
     8581 cgcccgaggc cgcttggtag gtcatgcagg tcatccactc gacgaggtcg ttttggaaca
     8641 ggccgtgcat gcccatcaac atgcagctta cggtgcaatt tccgccgatg taattcctga
     8701 cgcctcgtgc cagcgcgctc ttgatgaccg gcagattcac cgggtcgagc acgataatgg
     8761 cgtcgtcgtt catccgcagg gtccgggcgg cgtcgatcca gtagccgttc cacccggcgg
     8821 cgcgtagctt cgggtagatc tcgccggtgt agtcgctgcc ctggcaggac agcagaacat
     8881 cattggcagc gagcgccttg agatcgcgcg catccacgag cggcggcacg tccctgccga
     8941 cgtccggccc tttgcctccg gcctgcgaag tcgagaagaa aacgggttca atcaggtcga
     9001 agtcgcgctc agcgcgcatg cgctggagca acaccgagcc caccatgccg cgccagccga
     9061 tcaaacctac cgtagccatt gcagtagccc taattcaaga ggctccattg tatcaagggg
     9121 ttaagcactc aggacggctg gtgcaaaaaa gcaacaccct gccaccatgc cggcaatccc
     9181 ttagagagcg gccacgaccg catcgcccat ctcgcgggtg ccaattttct gcgtgccggc
     9241 ctcgtggata tcggccgtgc gaaatccctg cgcgagcacg gacttgaccg ccgcctcgat
     9301 gcggtaagcc cattcgtcct tgttgaacgt gtagcgcagc atcatggccg acgagaggat
     9361 ggttgcgagc ggatttgcga tattccggcc ggcaatgtcg ggggccgagc catggctggg
     9421 ctcgtacagt cccttgccgc tcgcatcgag cgaagccgag ggcagcatgc cgatcgagcc
     9481 ggtcagcatc gaagcttcgt ccgagaggat gtcgccgaac atgttgccgg ttacgatcac
     9541 gtcgaattgt ttcggcgcgc gcacgagctg catcgccgcg ttgtccacgt acatgtgaga
     9601 cagcgcaacc tgcggaaact tcttcgccac gtcgttgacg atctcgcgcc agaagcggct
     9661 cgtttcaagc acgttttcct tgtccaccga acaaagtttc ctgccgcgct tcatcgcggt
     9721 ggcaaatccg acttccgcta tgcgccggat ctcgggctcg ctgtagagca tggtgtcgta
     9781 gccttcgcgc tcgccgcgtg agttgtcgcg gcgcccgcgc ggctcgccga agtaaatgtc
     9841 tccggtcagt tcgcgaatga tcatgacgtc gagacccgac accacctcgg gcttgagcgt
     9901 cgaggcgccg acgagctcgg gaaacatcaa cgccgggcgc aggttggcga acaacgccag
     9961 ctccctgcgg atgcgcagta ttccctgctc cgggcgctgc gggcgcggca atgcatcgta
    10021 ctttggcccg cctaccgcgc cgcacaacac ggcatcggcc gccttggcaa gcttcaggct
    10081 cgattcgggc agcgggtcac cgtgggcatc cacggcggcg ccgccaaacg gcgcttcttc
    10141 cagctcgagc ttgagcccgt cgcgcgcgag cgcttccagc accttgacag cctgcgcgac
    10201 aatctccggc ccgatgccgt cgcctgcgag aaccgcgatc ttcattgatt gacctttatg
    10261 cagccgccag gacgccttgg cgccaaggaa gccacatcaa gcttgcctgt actttattgt
    10321 gaaattcttg gcgtctctgc gccttcgcgg ccaattcacc accgctctca gccgaacagc
    10381 cagggctggc gctcgcggtg cttcgcttcg aatgccctga tctccgcggc gtgccccagc
    10441 gtgagcccga tctcgtcaag cccgttcaac aggctgtgct tggcgaacgg atcaatgtcg
    10501 aacttgaagc tctcgccgcc cggggtggtc actgtctgtc tctcgaggtc cacggcaagc
    10561 cgatagcctt cggtccccgc ggcttccttg aacagccggt ctacagtcat ttcgtcgagc
    10621 ttgatcagca gcagaccgtt cttcaggctg ttgttgtaga aaatgtcggc aaaacttgga
    10681 gcgatcacgg tctggaatcc gtattgaagc agagcccatg gcgcatgctc ccgcgaggag
    10741 ccgcagccga agttctcgcg agccagcagc acggtggccc cgcggtagcg cggctggttc
    10801 agcacaaaat cgggattaag cggccgcttc gcgtggtcca ttccgggttc cccgtggtcg
    10861 agatagcgcc actcgtcgaa caggttcgga ccgtaaccgg tgcgcttgat ggacttcagg
    10921 tactgcttgg ggatgatcgc gtcggtgtcc acgttggcgc ggtccatcgg cgccaccagg
    10981 ccttcctgtc tgataaattt ttccatgagc gttccgttac tgggctttct tctggatttt
    11041 gtcgccgagt tgttccacgt ccttgccgag cccatgcatg gtattgcagc cggccagggc
    11101 gagggccagc gcaaccagaa taaacgcaag cggcttcttc atggcggcct ccgtcatttg
    11161 aagttccgaa catccacaaa atgtcccgca atggcggcag cagcggccat cgcgggactc
    11221 accaggtgcg tccgtcctcc cgggccctgc cgtccctcga aattgcggtt cgaggtcgag
    11281 gcgcagcgct cgccgggtga aagctggtcg tcattcatgc caaggcacat ggagcaaccc
    11341 ggctgccgcc actcgaagcc agcagccata aagatcttgt ccaggccctc cttctcggcc
    11401 tgctccttga ccagccccga accgggaaca acgatcgcca gcttgatgtt cgacgccacg
    11461 cgcttgtcct tgaccaccgc agccgccgcg cgcaaatcct cgatgcgcga gtttgtgcac
    11521 gcgccgataa ataccttgtc gggcctgatc ccggtgatag gcgtgttggg cttcagattc
    11581 atgtacttca gcgcgcgctc gatccactcg cgctttaccg ggtccttctc ctgcgccgga
    11641 tcaggcactt tcccgtcaat ggtcgtcacc atctgcggat tggtgcccca ggtgacctgc
    11701 ggctggatgg cggtggcatc gagcgtcacc acctcgtcga attttgcgcc ggcatcggaa
    11761 atcagcgtgc gccagtacgc ttccgccctg tcccacattg cgcctttggg cgccatcggc
    11821 cgacccttca cgtactcaag ggttttctcg tccaccgcta ccatgccagc gcgggccccc
    11881 gcctcgatcg acatgttgca cagggtcatg cgcccctcca tcgtaagcga gcggatcgcc
    11941 tccccgctga actcgatggt atacccggtg ccgccggcgc tgccgatctt tccgatgatc
    12001 gcgagaatca cgtccttggc ggtgatgccg cgcccgagcc tgccgtacac cacgacctgc
    12061 atgctcctcg tcttcttcat caccaggcac tgcgtcgcga ggacgtgctc gacctcgctc
    12121 gtgccgatgc cgaacgcgag gcaggcgaat gcgccatggg tgctggtatg cgagtcgccg
    12181 cacacgaccg tcattcccgg cagggtcgcg ccctgctccg gcccgatgac gtgcacgat
//
LOCUS       DBNGSG010000123         7855 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1230743, whole genome shotgun sequence.
ACCESSION   DBNGSG010000123 DBNGSG010000000
VERSION     DBNGSG010000123.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7855)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7855)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7855
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1230743"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="AC158Q_03165"
     CDS             <1..395
                     /locus_tag="AC158Q_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338797.1"
                     /translation="PWVQAGSPQPATDAESLLLYYQYVRKLSSAELGREHDAARQALS
                     RSRSDFNRVRFAMVLSNSGTAFYDEPRALELLDPVSKNPNGHLGGLAYLLISHLQERK
                     RLDANAQGLQQKLDALRSLERSLIERKK"
     gene            407..1780
                     /locus_tag="AC158Q_03170"
     CDS             407..1780
                     /locus_tag="AC158Q_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR3338798.1"
                     /translation="MEKTMETSASQRTSILVVDDDPDLLRLMQIRLTSAGYAVSTAES
                     GERALAQASVGRPQLLVTDLRMGGMDGMALFEAIRADHPTMPVIILTAHGTIPDAVAA
                     VQRGVFGYLTKPFDPKALLTEVERALAMAGGAHANGDAGGEWRRDIITRNPAMEDVLA
                     KARMVAESDASVFIQGDSGTGKELIACAIHKASPRRDHAFVAINCGAIPEQLLESELF
                     GHVKGSFTGAVRDNKGLFQTANRGTLFLDEIGDMPVSLQVKLLRVLQEKKVRAVGSTE
                     AHDVDVRVISATHRNLEAEMAAGNFREDLYYRLNVVALALPSLSDRREDIPLLANYFL
                     TTLAEKYKKSVNGFAPDALELMVAATWPGNVRQLYNVVEQSVALATTPIIPVSLAQQA
                     IQNQQAEFASFEDARRRFEREYLTQLLKITDGNVTQAARLAKRNRTEFYKLLGRHQLD
                     PKIFKGR"
     gene            2289..2891
                     /locus_tag="AC158Q_03175"
     CDS             2289..2891
                     /locus_tag="AC158Q_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HIR3338799.1"
                     /translation="MGTVDTLGNAEQKRPRILYPLMLIAAIVVIVFSVVGIATMMGWM
                     PSALSGGDPSAKPSYDTPPAPATQPAKSVRATPARPPVAASGPASCRDCGVIESIHVV
                     EAKGEGSGVGAIGGAVVGGVLGNQVGSGRGRTAATVVGAGAGAYAGHEIEKSMKKSVS
                     YQVRVRMNDGTYRTFHESTQPAFAIGQKVRVTEQGVLAAG"
     gene            2925..3092
                     /locus_tag="AC158Q_03180"
     CDS             2925..3092
                     /locus_tag="AC158Q_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338800.1"
                     /translation="MAEARHGIDTWDFGEILAALREIAREDAGRTEYAAAAEDEAIRC
                     LLPALTPGEGQ"
     gene            3100..3501
                     /locus_tag="AC158Q_03185"
     CDS             3100..3501
                     /locus_tag="AC158Q_03185"
                     /inference="COORDINATES: protein motif:HMM:NF023514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3568 family protein"
                     /protein_id="HIR3338801.1"
                     /translation="MNSRIATLLVAGGVGLAGCVPIGVTAFGVGASAGVAHGLGGVTY
                     RTFTAPMPKVRGATMTALDKMGIRVLSTSKVEGTEVIKASAKEREIEVALEPLTPSTT
                     RIRTVAKNGLFYDSATATEIILQTEKILGNV"
     gene            3513..4361
                     /locus_tag="AC158Q_03190"
     CDS             3513..4361
                     /locus_tag="AC158Q_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338802.1"
                     /translation="MNSVTTLLLAGSLAASCWFTLAQAQDSPPPAVVTGSSSQVETRL
                     VGSFSGFAGSDDNAQSLVTGLRQGSEITLTAAGTGGQAGETATFTPPTRPMGYGNVRI
                     SLALAQESLAQNGITQPTPEQLKAALAGGTIATGTGEAGATTQLPGILQMRADGMGWG
                     QIANSMGVKLGHVISGRTGQPAPDAPSTAASTAAGAGAGVTTAGQGSTTGQTQGRGNS
                     AAAHQSHRAGTGIVTAAGGSGAAIGAGARVNNAGGAGVVTGAGAAAGSRASAAGVAHG
                     KGHAKP"
     gene            4520..4768
                     /locus_tag="AC158Q_03195"
     CDS             4520..4768
                     /locus_tag="AC158Q_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338803.1"
                     /translation="MIGLALLAILLAGCAATEPRPRLVNQSGLVNQSGYSDTFKQGYA
                     AGCESAGGRVQQRNEGRYKTEADYMMGWNDGFSACRRR"
     gene            complement(4849..6966)
                     /locus_tag="AC158Q_03200"
     CDS             complement(4849..6966)
                     /locus_tag="AC158Q_03200"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIR3338804.1"
                     /translation="MQAAASALFKDWSGYLKPEDISQLESAFHFSEQAHEGQFRQSGE
                     PYISHPLAVASILAEWHLDSQALTAALLHDVMEDTSITKTEISRNFGRPVAELVDGVS
                     KLDKIEFESQAEAQAENFRKMLLAMARDVRVILIKLADRLHNMRTLDAVAPEKRRRIA
                     RETLDIYAPIANRLGLNSIYQELEDLALKHLYPDRFRVLAKAVKSARGNRREVVGKVL
                     DGIKKKLKSAGVEAQALGREKHLHSIYRKMREKHLTFAQVLDVYGFRIIVKDVPSCYL
                     ALGALHSLYKPIPGKFKDYLAIPKANGYQSLHTTLFGPFGTPIEVQIRTQEMHKIAEA
                     GVASHWLYKDADTSLSDLQKKTHQWLQSLLEIQSQSGDSAEFLEHLKVDLFPDEVYVF
                     TPKGRILSLPRGATAVDFAYAVHTDIGNRCVAVKINQELMPLRTELKNGDRVEIITAA
                     HAKPNALWLNFVATGKARSRIRHFLKTMHFQESVKLGERLLNQALAALGAAPAEIKEG
                     HWDKFVPDAGAKSRQEILADVGLGKRLAAVVARKLLSRGDPGPHETPAPGSVIIHGSE
                     GMAVQFAPCCRPIPGDPIIGVISKGQGMVIHTHDCPVIAKARHDPDRVLDVAWGAETR
                     KLFEVNIKLVVANQRGVLAKVAAEIAEAGSNIQNVAVDPEDGSSYTNMFFTLQVANRL
                     HLAGIMRALRRIPEVVRIVRVKG"
     gene            complement(7065..7274)
                     /gene="rpoZ"
                     /locus_tag="AC158Q_03205"
     CDS             complement(7065..7274)
                     /gene="rpoZ"
                     /locus_tag="AC158Q_03205"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HIR3338805.1"
                     /translation="MARITVDDCLKMIPNRFEMSLAATYRARQLANGAQPMMEASRDK
                     PTVIALRELAAGKFGVEILNKGQTG"
     gene            complement(7292..>7855)
                     /gene="gmk"
                     /locus_tag="AC158Q_03210"
     CDS             complement(7292..>7855)
                     /gene="gmk"
                     /locus_tag="AC158Q_03210"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - GMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HIR3338806.1"
                     /translation="KTSLVAALIRGEPTVKLSISHTTRPPRASEVDGRDYHFVAAAEF
                     ERMLEAGAFLESATIYGQQYGTSQQWVTDQLATGQDILLEIDWQGAQQVRRKMPGTIN
                     IFVLPPSLDALGERLRARSQDAPEQIARRLAAAREDISHFVEFDYVIMNDDFDRAAQD
                     LASIVRAERLRLHRQLARHADSINRMK"
BASE COUNT         1390 a         2473 c         2645 g         1347 t
ORIGIN      
        1 tgccctgggt ccaggccggc agtccccagc ccgctactga tgcggagagc ctgttgctct
       61 attaccagta cgtcagaaag ctttccagtg ctgaactggg ccgcgagcat gacgccgcgc
      121 gccaggcact ttcccgctcg cgctcggact tcaatcgcgt ccgtttcgcc atggtgcttt
      181 cgaattccgg caccgcgttc tacgatgagc cgcgcgcgct ggagctgctg gacccggtct
      241 cgaagaaccc gaacggccat ctgggcgggc tggcctacct gttgatctcg cacctgcagg
      301 aacgcaagcg gctcgacgcc aacgcgcagg gcctccagca aaagctcgat gcgctgagat
      361 cgctcgaacg cagcctgatc gaaaggaaga aatgatggaa aagataatgg aaaagacaat
      421 ggaaacgagc gccagccaac gcaccagcat actcgtcgtg gacgacgacc ccgatctgct
      481 gcggctgatg cagatccggc tgacgtccgc cggttacgcc gtgtccaccg cggagagcgg
      541 ggagcgcgcg ctcgcccagg caagcgtcgg ccgcccccaa ttgctggtga ccgatctgcg
      601 gatggggggc atggacggca tggcattgtt cgaggccatc cgcgccgacc acccgacgat
      661 gcccgtaatc atactcaccg cgcacggcac gattcccgat gccgtggccg cggtccagcg
      721 cggcgttttt ggctatctca ccaagccgtt cgatcccaag gcgctgctga ccgaggtcga
      781 gcgggccctg gcgatggcgg gcggggccca cgcgaacggc gacgcgggcg gcgaatggcg
      841 ccgggacatc atcacccgca accccgccat ggaggacgtg ctggccaagg cgcggatggt
      901 ggccgaaagc gacgccagcg tattcataca gggcgactcg ggcaccggca aggaactgat
      961 cgcgtgcgcg attcacaagg cgagcccgcg gcgcgaccac gccttcgtcg cgatcaattg
     1021 cggggcgatt cccgagcaat tgctcgaatc cgagctgttc ggccacgtga agggctcctt
     1081 cacgggcgcg gtgcgcgaca acaaggggct gttccagacc gccaaccggg gcacgctgtt
     1141 tctcgacgag atcggcgaca tgccggtctc tctccaggtg aagctgctgc gcgtgctgca
     1201 ggagaaaaaa gtgcgggcag tcgggtcgac cgaggcgcac gacgtcgacg tgcgcgtgat
     1261 ctccgcgacc caccgcaacc tcgaggcgga gatggcggcg gggaacttcc gcgaggacct
     1321 ttattaccgg ttgaacgtgg ttgcgctggc gctgccgtcg ctctcggacc ggcgcgagga
     1381 tatcccgctg ctcgccaatt atttcctgac gacgctcgcc gagaaataca agaagtccgt
     1441 caacggcttc gcgccggacg ccctggaact gatggtggcg gcgacctggc cgggcaacgt
     1501 gcgccagctc tacaacgtgg tcgagcagtc ggtggcgctc gccaccacgc ccatcatccc
     1561 ggtatcgctc gcgcagcagg cgatccagaa ccagcaggcc gaattcgcct ccttcgagga
     1621 cgcccggcgc cgcttcgagc gcgagtacct gacgcagctg ctgaaaatca cggacggcaa
     1681 cgtgacgcag gcggcgcggc ttgccaagag gaaccgcacc gagttctaca agctgcttgg
     1741 gcggcaccag ctcgatccca agatcttcaa gggccgttaa acccggacat cgcgagcgcc
     1801 ttggcgctcg cgatgccggt ttgaccgcgc gggatcgaac ggacgcccgt ccgcgcggga
     1861 tttcagccat cgcatccccg taaacgccgt tcatctcgcg ccgcggacgg gatccggcca
     1921 cgacgcgctc cgtgacggat gcacgatgcg cgcctttccc gatcgggtgg tcccgggcgc
     1981 gtaaccgccg cgtttggtca ccgctgtcgc cgccgcacga cagaaaaacc ccagccgaat
     2041 cacccgggta cgccgggtgt gatgctgttt gttgccgaac aacgacattc gaccccggga
     2101 catccatggc aactaattga tttaacagaa cgaattgacc tggcacggca gttgcccttt
     2161 caactcataa acacgggtag gtgctcgccg tttgcgtgaa ctaagcccgg tcaactaaac
     2221 aagaaccggg tgctggatcg tcgtgcaggg tgcgcgacgg caacttcacg caaggccaaa
     2281 ggaatgaaat gggaactgtc gatacccttg ggaacgcgga gcagaaacgc cccaggattc
     2341 tgtatcccct gatgttgatt gccgcgatcg tggtgatcgt gttcagcgtg gtgggcattg
     2401 ccaccatgat gggctggatg ccgtccgcgc tgtcaggcgg cgatccgtcg gcaaaaccgt
     2461 cgtatgacac cccgccggct cctgccacgc agcctgccaa atccgtccgc gccacgcctg
     2521 cccggccgcc ggtggccgcc agcggtccgg caagttgccg tgactgcggt gtgattgaat
     2581 cgatccatgt ggtcgaggcg aaaggcgagg gcagcggcgt cggagctatc ggcggcgccg
     2641 tggttggcgg tgtcctcggc aaccaggtcg gcagcggcag ggggcgcacc gcagccacgg
     2701 tcgtaggcgc cggtgccggc gcctacgccg ggcacgagat cgagaaaagc atgaagaaat
     2761 ccgtcagcta ccaggtgcgc gtgcgcatga acgacggtac ttaccggacg ttccacgaga
     2821 gcacgcagcc cgcatttgcc atcggccaga aggtgcgcgt caccgagcag ggagtccttg
     2881 ctgcgggttg aggcgctgcc agcggataac cagaggagga cgtcatggca gaagctagac
     2941 acggaatcga tacctgggac ttcggcgaga tcctggccgc actgcgcgag atcgcgcgcg
     3001 aagatgccgg caggacggaa tatgcggcgg cggcggagga cgaggccatc cgttgcctcc
     3061 tgcccgcgtt gacccccggc gaagggcagt gagcgtggca tgaacagccg gatcgcgaca
     3121 ctgctggtcg ccgggggcgt ggggctcgcc ggctgcgtgc cgatcggcgt taccgcattc
     3181 ggcgtcggcg cctccgccgg tgttgcgcat ggcctgggcg gcgtcactta tcgcaccttc
     3241 actgcgccga tgccgaaggt gaggggtgcg acgatgaccg cgctcgacaa gatgggcatc
     3301 cgggttctat ccacctccaa ggtcgaaggc accgaggtaa tcaaggcgag cgccaaggaa
     3361 cgcgagatcg aggtggctct cgagccgctg acgcccagca ccacgcgcat ccgcacggtg
     3421 gcgaagaatg gattgttcta cgacagcgcg accgccaccg agatcattct tcaaaccgag
     3481 aagattctgg gtaacgtgta aggaggcctg tcatgaattc cgtcacaacg ttgttgcttg
     3541 cgggttccct ggccgcctcg tgctggttca cccttgcgca ggcacaggac tcgccgcctc
     3601 ccgctgtcgt gacgggcagc agttcgcagg ttgaaacccg tcttgtcggc agtttctcgg
     3661 gctttgccgg gtcggatgac aacgcgcaga gcctggtaac gggcttgcgc cagggaagcg
     3721 agattacgct gaccgcggcg ggcacgggcg gacaggccgg agagaccgcc acgtttacgc
     3781 cgcccacccg ccccatgggg tatggcaacg tgcggatttc gctcgcgctg gcgcaggaat
     3841 cgcttgccca aaacggcatc actcagccga cgcccgagca gctcaaggcg gcgctcgccg
     3901 gcggaaccat tgcaacgggt acgggcgaag ccggcgccac gacgcagttg cccggcattc
     3961 tgcagatgcg cgccgacggc atggggtggg gccagatcgc caacagcatg ggcgtgaaac
     4021 tcggccacgt cataagcggc aggaccggcc agccggcgcc ggacgcgccg tccaccgcgg
     4081 cctcgacggc ggctggcgca ggcgccggcg tgaccacggc ggggcagggt tccaccacgg
     4141 ggcaaacgca gggccgcggc aacagcgcgg cggcgcacca gtcgcatcgc gccgggaccg
     4201 ggatcgtgac cgctgccggc gggtccggtg ccgcgattgg cgcgggtgcg cgtgtcaaca
     4261 atgcgggtgg cgcgggcgtg gtgaccgggg ccggcgcagc cgcaggcagc cgcgcttccg
     4321 ccgcgggcgt tgcgcacggg aaaggccacg ccaagccgta gacaagcgtg agttccggcg
     4381 cgggccggta cgatgcgcgt cgcgggcaag cagaaccaac cgctcgcgaa gtccgcttga
     4441 aaccaggcaa cgtgatccgt attccgcgtc tttaaacgcc gcggctggag gagatatgac
     4501 gagcgggaac gggacatcct tgattggcct ggcgctgctc gcaatactgc tcgcgggatg
     4561 cgctgcaacg gagcccaggc cgcggctcgt caaccagagc gggctcgtca accagagcgg
     4621 gtactccgat acgttcaagc aaggttatgc cgcgggctgc gagagcgccg gcggccgcgt
     4681 gcagcagcgc aatgaaggcc gctacaagac tgaagccgac tacatgatgg gctggaacga
     4741 cgggttcagc gcctgccggc gccgctagcg cggcgagtgt tgagttttga gtgatgaatt
     4801 ttgagttgaa ctcaacacta aaaactcaaa actcaaaact gaatcccctc agcctttgac
     4861 cctgacgatg cggacgactt ccggaatgcg cctgagcgcc cgcatgatgc cggcgaggtg
     4921 caggcgattc gccacctgaa gcgtgaagaa catgttggtg taactgctgc cgtcttcggg
     4981 atccaccgcc acgttctgga tgttggagcc cgcttccgcg atctcggccg ccaccttggc
     5041 gagcacgccg cgctggttgg ccaccaccag cttgatattg acttcgaaca gcttgcgggt
     5101 ctcggcgccc cacgccacgt cgagcacccg gtcggggtcg tggcgcgcct tggcgatgac
     5161 cgggcagtcg tgggtgtgga tgaccatgcc ctgacccttg ctgatgacgc cgatgatcgg
     5221 gtcgcccggg atcggccggc agcaaggggc aaactgcacc gccatgcctt ccgagccgtg
     5281 aatgatgacc gagccgggcg caggcgtctc gtgcggcccg ggatccccgc gcgagagcag
     5341 ttttctcgcc acgaccgcgg cgagccgctt gccgaggccg acgtcggcaa ggatttcctg
     5401 gcgcgacttg gcgccggcgt cgggcacgaa cttgtcccaa tgcccctcct tgatttcggc
     5461 gggcgcggcc ccgagcgcgg ccagcgcctg gttgagcagc cgctcgccga gtttcacgga
     5521 ttcctggaaa tgcatggtct tgaggaagtg gcggatgcgc gagcgcgcct tgccggtcgc
     5581 cacgaaattg agccagaggg cattgggctt ggcgtgcgcc gcggtaatga tctcgacgcg
     5641 gtcgccgttt ttcagctcgg tccgcaacgg catcagttcc tggttgatct tcaccgcaac
     5701 gcagcggttg ccgatgtcgg tgtgaacggc gtaggcgaag tcgaccgccg ttgcgccccg
     5761 cggcagcgac aggatcctgc ccttgggggt gaacacgtag acctcgtccg ggaagagatc
     5821 caccttgaga tgctcgagga actcggccga gtcgccggac tgggactgga tctcgagcag
     5881 gctctgcagc cactggtggg ttttcttctg caggtcggaa agcgaggtgt ccgcgtcctt
     5941 gtagagccag tgcgaggcca ccccggcctc cgcgatcttg tgcatctcct gggtgcgtat
     6001 ctggacctcg atcggcgtgc cgaacgggcc gaacagcgtg gtgtgcagcg actggtagcc
     6061 gttcgccttg gggatggcga gatagtcctt gaacttgccc gggatcggct tgtagaggct
     6121 gtgaagcgcg cccagcgcga ggtagcagga cggcacgtcc ttgacgatga tgcggaaacc
     6181 gtagacgtcg agcacttgcg cgaaagtgag gtgcttctcg cgcatcttgc gatagatcga
     6241 gtgcaggtgc ttttcgcgcc cgagcgcctg ggcttccacc ccggcgctct tcagcttttt
     6301 cttgatcccg tccagcacct tgcccaccac ctcgcgccgg ttgccgcgcg ccgatttcac
     6361 cgccttggcg agcacccgga agcgatcggg atagagatgc ttgagcgcga ggtcctcgag
     6421 ttcctggtag atgctgttca gtcccaggcg gttggcgatg ggggcgtaaa tgtcgagcgt
     6481 ctcgcgtgcg atgcggcgcc gcttctcggg cgcgaccgcg tcgagcgtgc gcatgttgtg
     6541 cagccggtcg gcgagcttga tcaggatgac gcgcacgtcg cgcgccatcg ccagcagcat
     6601 cttgcggaaa ttctcggcct gggcctccgc ctgcgactcg aattcgatct tgtcgagctt
     6661 ggacacaccg tccaccagct cggcgaccgg tcttccgaag ttgcgggaga tctcggtctt
     6721 ggtgatcgac gtgtcctcca tcacgtcatg gaggagggcg gcggtgagcg cctgcgagtc
     6781 gagatgccac tcggcgagaa tgctggcgac cgcgagcggg tgcgagatgt agggttcgcc
     6841 cgactggcgg aactgcccct cgtgcgcctg ctcgctgaag tggaaagcgg actcgagctg
     6901 cgagatgtcc tccggcttga ggtagccgga ccagtccttg aacaacgcag aggcggcggc
     6961 ttgcatctac gtattgatga gtgatgagtg atgagcgatg agtgatgagc gaaaagcagg
     7021 acctcccgcg gcccgccccg tcctcagtcc gcattcatca tgggctatcc ggtctggcct
     7081 ttattgagaa tttccactcc gaatttgccg gcggcgagct cgcgcagcgc gatcacggtg
     7141 ggcttgtcgc ggctcgcttc catcatcggt tgcgcgccgt tggcgagctg gcgcgcgcgg
     7201 taagtcgccg cgagcgacat ttcgaagcgg ttgggaatca ttttcaggca gtcgtcgacg
     7261 gtgatgcgtg ccataagagt cggagctccg gttacttcat gcggttgatc gagtcggcgt
     7321 ggcgggcaag ctgccgatgc agcctgagcc gttcggcccg gacgatgctt gccagatcct
     7381 gcgctgcccg gtcgaagtcg tcattcataa taacatagtc gaactcgacg aaatggctga
     7441 tgtcctcgcg cgccgccgcc aggcgtcgcg cgatttgctc cggagcgtcc tggctgcggg
     7501 cgcgcagccg ctcaccgagc gcgtcgagcg agggcggcag cacgaaaatg ttgatggtgc
     7561 ccggcatctt ccggcgcacc tgctgagcgc cctgccagtc gatttcgagc aggatgtcct
     7621 ggccggtcgc cagctgatcg gtcacccatt gctgcgaagt gccgtattgc tggccgtaga
     7681 tcgtggcgct ctcgaggaat gcccccgctt ccagcatgcg ctcgaactcg gccgcggcga
     7741 cgaaatgata gtcgcggccg tcgacctcgc tcgcccgcgg cgggcgcgtc gtgtgcgata
     7801 tcgacagctt cactgtcggc tcgcctcgga tgagcgcggc aacgaggctc gtctt
//
LOCUS       DBNGSG010000124         2177 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1910908, whole genome shotgun sequence.
ACCESSION   DBNGSG010000124 DBNGSG010000000
VERSION     DBNGSG010000124.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1910908"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2177
                     /locus_tag="AC158Q_03215"
     CDS             <1..>2177
                     /locus_tag="AC158Q_03215"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-linked N-acetylglucosamine transferase,
                     SPINDLY family protein"
                     /protein_id="HIR3338807.1"
                     /translation="GSSLLRAVGLDELVGKDVDGFVAICKALASDPNRLAEMRKTLRE
                     RVTHSPLRDEAGFTKALEDRYVEMWHARLKGETVATLDADTIHALFQRAENHRKSGRP
                     AEAGELYKEILQDSPDHIEALTAIWDLSFETGNPGSAVDWLNKAISTRGDVASFHYML
                     GCSLQARGNMHDAIGSFSRALELDPRWGKAHNNLGCTVESTGDLVRALECYRRAVEFD
                     PKLAVAHYNQGNAYRQLGELVQAIHCIRQALAIDPGHADWRCNLGDLLFDRLQLDEAL
                     SSYQMAVELDPGYAVALAGLGRVLQVLGRPGEAESKFRKAIGLKPDLATASSGLLVSL
                     HYSRRADPQAILDEHKAWDARHARTGGWWAARTEDERHAQGRLRIGYLSPDFCRHPVA
                     SFIEPVLRAHDRARVQVFCYSNVAHPDEVTRRLEGLCDHWRDISGSSDEQVSERIRYD
                     GIDILVDLAGHTGGGRLELFARKPAPVQVTWLGYPDTTGLRAMDYRLTDAFADPMQVT
                     DRFHTEKLVRLANGFLCYQPPADVPEPGEPPSLRTGRVTFGSFNNLAKVTASMVEIWA
                     QLLHRMPNTKLLIKAFGLSADSAKQALEARFADHGIPAERLTLLPPESSTRSHLSRYH
                     DIDIALDSFPYNGTTTTCEALWMGVPVISLAGNDHASRVGVSLLNRVGLSDLVTHNPD
                     EYLAKAVELAQDAARLGELRGGMRQRLRASPLLDAPGFARGLEA"
BASE COUNT          418 a          676 c          690 g          393 t
ORIGIN      
        1 ggatccagcc tgctgcgcgc ggtcggtctc gatgagctgg tggggaagga cgttgacgga
       61 ttcgtcgcca tctgcaaggc gcttgcctcc gatccgaacc ggctcgcgga gatgcggaaa
      121 acgctgcgtg agcgcgtcac ccattcaccg ctgcgcgacg aagccggatt taccaaggca
      181 ctggaagacc gctacgtcga gatgtggcac gcccgtctga aaggcgagac cgtcgccacg
      241 ctggacgccg atactatcca tgcgctgttc cagcgcgccg aaaatcatcg gaagtcgggc
      301 aggccggcag aggcgggcga gctctacaag gagatacttc aggacagccc cgatcatatc
      361 gaggcgctaa cggctatctg ggatctatcc ttcgagaccg ggaacccggg ctccgcggtg
      421 gattggctga acaaagccat ctcgacgcgc ggcgatgttg catcgtttca ctacatgctc
      481 ggctgttcgc tccaggcgcg gggaaacatg cacgatgcca tcgggtcatt ttcccgggcg
      541 cttgagctcg atccccggtg gggaaaggcg cacaacaatc tcggctgcac ggtcgagtcg
      601 acgggagatc tcgtgcgcgc gctggaatgc tatcgccgcg ccgttgagtt tgacccgaaa
      661 ctcgccgttg cacattacaa ccagggcaac gcataccgcc agctgggtga actggtgcag
      721 gccatccact gcatccggca ggcgctggcg atcgatcctg gccatgcgga ttggcgctgc
      781 aatctgggcg atttgctctt tgaccgcctg caactggacg aagcgctttc cagttatcag
      841 atggccgtcg agctcgatcc cggttatgcg gtggccttgg cggggcttgg aagagtgctg
      901 caggtactgg ggcgcccggg cgaggcagaa tcgaaattcc gcaaggccat cggcctcaag
      961 cccgatctcg cgacagcgag cagcggactg ctcgtttcac tgcattacag ccggcgggcc
     1021 gacccgcagg cgatactgga tgaacacaag gcatgggatg cccgccatgc gcgcaccggc
     1081 ggctggtggg cggcgcgcac cgaagacgag cggcatgcgc agggccgact caggattgga
     1141 tacctgtcgc cggacttctg ccggcaccct gtggccagtt tcatcgagcc ggtgcttcgc
     1201 gcgcatgacc gcgcgcgggt ccaggttttc tgctattcaa atgtcgccca tcctgacgag
     1261 gtaacccgtc gcctcgaagg actgtgcgat cactggcgcg atatttcggg ctcgagcgac
     1321 gaacaggtca gcgagagaat ccgatacgac ggcattgata ttctggtcga cctcgccggt
     1381 cacacgggcg gaggcaggct ggaactcttc gcccgcaaac cggcgccggt gcaggtgacg
     1441 tggcttggct atccggacac gacaggcctg agggcaatgg attaccgcct cacggacgca
     1501 ttcgccgatc ccatgcaggt cacggatcga ttccataccg agaagctggt acgcctcgca
     1561 aacgggtttc tctgttatca gccaccggcc gatgtcccgg aaccggggga gccgccgtct
     1621 ttgcgcaccg gacgcgtcac gttcggatcg ttcaataacc ttgccaaggt gacggcctcc
     1681 atggtcgaaa tctgggcgca gttgctccat cgcatgccga ataccaaact gctgatcaag
     1741 gcattcggac tgtccgccga cagcgcaaag caagccctgg aggcccgatt cgcggaccac
     1801 ggcataccgg ctgaacgact caccctcctg ccgccggaga gctctacccg gtcacaccta
     1861 agccggtacc atgatattga cattgcgctg gactcgtttc cgtataacgg cacgacaacg
     1921 acctgcgaag ccttgtggat gggcgttccg gtcatctcgc tcgcaggcaa cgaccatgca
     1981 tcgcgggtag gcgtgagcct attgaaccgg gtcggactct ccgacctggt cacccacaat
     2041 cccgacgagt acctggccaa ggcggtggaa ctcgcgcaag acgccgcacg cctgggtgaa
     2101 ttgcgcggtg gcatgaggca gcggctgcgc gcttctccgc tgctcgatgc cccgggattt
     2161 gcgcggggac tggaagc
//
LOCUS       DBNGSG010000125         9350 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2251193, whole genome shotgun sequence.
ACCESSION   DBNGSG010000125 DBNGSG010000000
VERSION     DBNGSG010000125.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9350)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9350)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9350
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2251193"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="AC158Q_03220"
     CDS             <1..474
                     /locus_tag="AC158Q_03220"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HIR3338808.1"
                     /translation="YLAANLAVVFAQLGGKTLLIDGDMRNPRQHRIFGQGNGPGLAQV
                     LSGRLGAEAAVRVPYFDNLWLLPAGAAPPNPLELLSSPAFPRMLLEFRGQFDLILIDT
                     PAAARGSDARVIGARADGALVIARTDRTRAGDLERLCRTMGASGTPVVGTVLNRY"
     gene            505..1497
                     /locus_tag="AC158Q_03225"
     CDS             505..1497
                     /locus_tag="AC158Q_03225"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase family protein"
                     /protein_id="HIR3338809.1"
                     /translation="MDIGYANTGGSGFHAHSRIYVAGHRGMVGSALVRRLRARGYANL
                     ILRTRQELDLADQAAVGRFMERAEADYVFIAAARVGGIVANNTYRGEFIYQNLVVQTN
                     LIHAAMRAGVRRLMFLGSSCVYPRDCPQPIREEYLLTGPLERTNEPYAVAKIAGIRMC
                     EAYNAQYGTRYVAVMPTNLYGPNDNYDPVTAHVLPALLRKAHLAKVEGAGSLAVWGSG
                     RPRREFLHVEDMADACILLMERGAGEGLYNIGSGTDVTIRELAEIIARVVGFDGRLAF
                     DSSKPDGTPRKLLDVSRLNALGWHPRLGLEEGIRMTYRNCLENGVFGLAEAEHG"
     gene            1494..2897
                     /locus_tag="AC158Q_03230"
     CDS             1494..2897
                     /locus_tag="AC158Q_03230"
                     /inference="COORDINATES: protein motif:HMM:NF014051.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HIR3338810.1"
                     /translation="MTFKFPASLAVATGTHADAAGRAATRLAARVADDFSRLRGALRR
                     AGWALADQCMVSAANFLTIFLFARHLEAATFGAFMLAYTGLLLLTNLQNALIVQPHNV
                     LAAGMAPPEYRRFTGALLVLQVLYGLAVCAGFFLVGWLIATAYSPATGDVLIALALTA
                     VPWMAQDFARRVLYTRGESRAAAANDGVTNGLQLFGALMLIQLPAAWAAPASALSVLG
                     LSAAAGALFGAWQLRNHVGFLGAGFGPYRHAWFEAWHFGKWLTAQNGLAWFGAQGHSW
                     VVGLMLGVEQVGIYRAATHLVNLMNPLLQACLTYLPSRGSLAYHAGGAAGLSRWVGRT
                     QRMLLLALAPFCVLLVVFPGQVLDLAYGGRFAGTTLALILALAAVAQCIGFSKYPFDI
                     GLLALRSPKSIFYAYLIPVILLLTVGASLIWYLGILGVPLSGIVINSALLIATRRAYR
                     ARMRQEQERPAVAKETL"
     gene            2894..4120
                     /locus_tag="AC158Q_03235"
     CDS             2894..4120
                     /locus_tag="AC158Q_03235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016145.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HIR3338811.1"
                     /translation="MNAPLNTAAVLPAGDERRARPEAAVFGEWNTSNLGDRGIHDGVL
                     RFFSECGWDARSYGLSTLTPVPADAASRPASAAAPGRVVGALGRVPPLKRVLRGARQR
                     VRIAALSPRLRHSQAIVVGGGALLSDANLHFPQSLVRLARAARRLGKPLVCLGCSVEG
                     EWSAPGSRMIGEFLDACEVIAVRDHATAGRLAAVIGAPPPVFGDFCLWESRVMEGGGS
                     PQERHSYAINVSQLPGPWAGAQEHYEDALVALISRLARRAQGRRAGIRIFTTGTAEDA
                     RAAQRVFGRIDAVGAELHLPGNLAQLSSMLRTSAVAVSTRLHAAILALAEQTPVVGFS
                     AGPKLRNYFATLGIRRCAYDVADQAQLMDWLAQADYGELYSEQRRALVHSPIWADRAR
                     VRRSLELLAGAETTCR"
     gene            4120..5250
                     /locus_tag="AC158Q_03240"
     CDS             4120..5250
                     /locus_tag="AC158Q_03240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3338812.1"
                     /translation="MLYLKHFPAAGAPLNDGTSIAVGGLASGLAENGARVAVLCEGPA
                     RSSITTERGYAVECFANRHPYRTFSLAPGLKRHVAEHLASRPGLCLVNGMFHPPVYAM
                     GRWLRRLGVPYVVAPHDPYDSTVFSRNAHLKWPYWYLFERRLLARASAVQVLDIQHAD
                     CLRGLGIGTRVIETLNGVAPGSAPPESQLAWRSAREPVSLMFLGRIDSWNKGLDILID
                     AFPHFAALTAARLTVQGPDWGDRARLEELAARRMISSKVAFHGPDYRRPAPWIIADHD
                     VFCLPSRFEGFGLAALEAMLAARVLLVSEIAGIARHVRASDCGVTVKPTVAGVAEGLA
                     ALLRRRSEWREMGLRGRRYALANLQWKNIAASALRQYERLAA"
     gene            5256..7061
                     /locus_tag="AC158Q_03245"
     CDS             5256..7061
                     /locus_tag="AC158Q_03245"
                     /inference="COORDINATES: protein motif:HMM:NF012360.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338813.1"
                     /translation="MNDRQYVAQCLFQYLDHCEVAYCVVGDTRDYPEAIRSDIDIVVP
                     GWMLDDLPRLAARFCRELDIHLVQLIRHEQTALYLVFAWFGESGEARFLAVDFCSDYL
                     RGGRLLLGAEELLDHRGPAITRRGTNQGFRVPAPHMQFIYYLVKKVDKQDLDDGHGEY
                     LSGVWAMDPEGCWSQLNRFWPAERDAELLAEAAARNDWYEVQAELPRLKRALRRVVSL
                     SPLHAARELRRWIARVLRPTGLVVAFLGADGSGKSSVIERVLTDLAPAFRHTRYLHLR
                     PRLFSRAGGEERPVTAPHALPARGRAASLAKLAYFLFDYLFGHAFHVWPLKCRSTLVV
                     FDRHYRDLAIDARRYRYGGPMRLARWAARLAPGPDFWVLLDAPAEILQSRKREVPAEE
                     SERQRHAYLEAASHLHDAAVVDASQTLEHVVADTESAILRWMEGRLEFRHPELQFERN
                     PVTARLLQYFCRNKTPLLAKLMRIILNSDVHCRIHSPILMAHPYGIIIHADSVIGRRV
                     AIMQQVTLGGKDWGVNEAPVIEDDVYIGAGAKVLGAVRVGRGAIIGANAVVTRDVPPH
                     CTVVGANRIVRQPKYARSDANAVEFRPPPDERLSA"
     gene            7087..8436
                     /locus_tag="AC158Q_03250"
     CDS             7087..8436
                     /locus_tag="AC158Q_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008754 - O-antigen ligase activity
                     [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR3338814.1"
                     /translation="MKPTAFGPAKEFPSASTDGGLLWTDYWYLAATAVAAAVAVDPLE
                     WGLASDPVIKHLALAISLPALVLTFAGSNLSVNSRRGGPLAVLWPLLALAIIALGGSL
                     YARAIEGIQNSFLNIGLYMLTAYSAAVMMLRSRAPEALARAQLRILLAAAAVMAVYLI
                     ANFRVRQVYHEQIFLVIPMAVLFFAGSLPAFVRWAGCLFFLAMAWFSAKYTSYLIGAA
                     TAAYLVLFVAIPRLTPRSGLRRTMLAYWICVLGAAAALGVVLLGLRGSFELPTGNVDY
                     RLHTYTAAWERFLDSPLWGTMFAVEAVQKFTLYSTGVARNLLATHSDVLDLLANGGLL
                     GIVLWLYALARIARAAFANLLRPGLFEHAWAPHAHTLAVMSLAGIITYAFNPILLQPA
                     LAYLVWTNLGMLAGLALRMQTADAPGGGLPHPAAARPWPARGAHGVAGAGFHARSRT"
     gene            8436..9161
                     /locus_tag="AC158Q_03255"
     CDS             8436..9161
                     /locus_tag="AC158Q_03255"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HIR3338815.1"
                     /translation="MSILLEPVRDGGRTAAAPRASPFSLRKLWLFVLGLPKTLLFNFS
                     CLPFRQALQLPVLVSHRVWLKDLSGKVSLADCRTGAVRIGFGDVGIFDPQRSRTIWQV
                     PGHVAFMGTADIGHGSRISVTGELVLGNRLIIAAESAIVAQRSIRIGNDVLISWDVLI
                     IDTDAHPIYDATGNQVNAAMPIRIGNSVWLGCRALVLKGAEIADGVVVAAASTVTRPV
                     LTPNAIVGGNPARVVRENVRWKR"
BASE COUNT         1318 a         3141 c         3284 g         1607 t
ORIGIN      
        1 tacctggccg cgaacctcgc cgtggtattc gcgcagctgg gcgggaaaac gctgctgatc
       61 gacggcgaca tgcgcaatcc gcgccagcac cgcatcttcg ggcagggcaa cgggccggga
      121 ctcgcgcagg tattgagcgg ccggctgggc gcggaggccg cggtgcgcgt gccgtatttc
      181 gacaacctct ggctcctgcc cgcgggcgcg gcgccgccca atccgctcga attgctgagt
      241 tcccccgcat ttccgcggat gctgctggaa tttcgcgggc agttcgacct gatcctgatc
      301 gacacgccgg cggccgcgcg cggctccgat gccagggtca tcggcgcccg cgccgacggc
      361 gcgctggtca tcgcccgcac cgatcgcacg cgggccgggg acctcgagcg cctgtgccgg
      421 acgatgggcg cgagcggaac cccggtggtc gggacggtcc tgaaccggta ctgagcgcgg
      481 cccgagactg cagggtgatg caacatggat atcggatacg cgaataccgg gggtagcggt
      541 ttccacgccc actcccgcat ctacgtggcc ggccaccgcg gcatggtcgg ctcggcgctg
      601 gtgcggcggc tgcgggcaag gggctacgcc aacctgatcc tgcgcacgcg gcaggagctc
      661 gatctggccg accaggccgc ggtcgggcgc ttcatggagc gtgccgaggc ggattatgtc
      721 ttcatcgccg cggcgcgggt cggcggcatc gtcgcgaaca acacctaccg cggcgagttc
      781 atctaccaga acctggtcgt gcagacgaac ctcatccacg ccgcgatgcg cgccggggtc
      841 aggcggctga tgttcctcgg ttcgagctgc gtctatccgc gcgattgccc gcagccgatc
      901 agggaggaat acctgctcac cggtccgctg gagcgcacca acgagccgta cgcggtggcg
      961 aagatcgcgg gcatcaggat gtgcgaggcc tacaacgcgc agtacggcac gcgctacgtg
     1021 gcggtcatgc cgaccaatct ctacggcccg aacgacaact acgacccggt cactgcccac
     1081 gtcctgcccg cgctgctgcg caaggcgcac ctcgcgaaag tggaaggcgc gggcagtctc
     1141 gcggtctggg gttccggcag gccgcgccgg gagttcctgc acgtggaaga catggccgac
     1201 gcctgcatcc tgctcatgga acggggcgcc ggcgagggtc tctacaacat cggcagcggg
     1261 accgatgtga cgatcaggga actcgccgaa attatcgcgc gcgtggtggg gttcgatgga
     1321 cggctcgcgt tcgactccag caagccggac ggcacgccgc gcaagctcct ggatgtcagc
     1381 cgcctcaatg cactgggctg gcacccgcgc cttggcctgg aggagggcat acggatgacc
     1441 taccggaact gcctcgagaa cggcgtgttc gggctggcgg aagcggaaca cgggtgacgt
     1501 tcaagtttcc ggcgagcctg gcggtcgcga ccggcaccca cgctgacgcc gccggccgcg
     1561 ccgcgacccg cctggctgcg cgcgtcgcgg acgacttctc gcgcctgcgc ggggcgctgc
     1621 gccgggcggg ctgggcgctc gccgaccagt gcatggtgag cgcggccaat ttcctcacca
     1681 tcttcctgtt tgcccgccac ctggaagccg cgaccttcgg cgcgttcatg cttgcctaca
     1741 ccgggctgct gctgctcacc aacctgcaga acgcgctgat cgtgcagccc cacaacgtgc
     1801 tggcggcggg gatggcaccg ccagaatacc gcaggttcac gggggcgttg ctggtgctgc
     1861 aggtgctgta cgggctcgcg gtttgcgcgg gatttttcct cgtggggtgg ctgatcgcaa
     1921 cggcgtactc gccggcgacc ggcgatgtcc tgatcgccct tgcgctcacc gccgtgccct
     1981 ggatggcgca ggatttcgcg cgccgcgtgc tctatacgcg cggtgagtca cgggccgccg
     2041 cggccaacga cggcgtgacc aatggactgc agttgttcgg cgcgctcatg ctgatccagc
     2101 tgccggccgc ctgggcagcg ccggcttcgg cactgtcggt gctcggcctt tcggccgccg
     2161 ccggcgcgct gttcggggcc tggcagctgc gcaatcacgt cggcttcctg ggagcgggat
     2221 tcggcccgta ccggcacgcg tggttcgagg cctggcattt cggcaagtgg ctgacggcgc
     2281 agaacgggct ggcatggttc ggcgcgcagg ggcattcgtg ggtggtcgga ctcatgctcg
     2341 gcgtggagca ggtcggcatc tatcgcgcgg cgactcacct cgtcaacctg atgaaccccc
     2401 tgctgcaggc gtgccttacc tacctgccct cgcgcggcag ccttgcgtat cacgcgggtg
     2461 gcgcggccgg gctgtcgcgg tgggtggggc ggacgcagcg gatgttgctg ctcgcgctgg
     2521 cacccttctg cgttctgctc gtggtgtttc ccggccaggt gctggatctc gcctacggcg
     2581 gtcgtttcgc gggaacgact ctcgcgctga tcctggcgct cgcggcggtc gcgcagtgca
     2641 tcgggttctc gaagtatccg ttcgacatcg gtctgctcgc gctgcgctcg ccgaagtcca
     2701 ttttctacgc ctacctgatc ccggtgatat tgctgctcac cgtgggcgcg tcgctgatct
     2761 ggtacctggg gatcctgggc gtgccgctgt cgggcatcgt gatcaattcg gcgctgctga
     2821 tcgcgacccg ccgcgcgtac cgggcgcgca tgcggcagga acaggagcgc ccggcagtcg
     2881 cgaaggagac cttatgaacg caccgctaaa tacggctgcc gtgctgccgg ccggtgacga
     2941 gcggcgtgca cggcccgagg ccgccgtctt cggcgagtgg aacacgtcca acctgggcga
     3001 ccgcggaatt cacgacggcg tcctgcggtt tttctccgaa tgcggctggg acgcgcgctc
     3061 ctacgggctt tcgacgctga cgccggtgcc cgcggacgcg gcgagccggc cggcgtcggc
     3121 ggccgctccc ggccgggtgg tcggggcact ggggcgcgtg ccgccgttga agcgcgtgct
     3181 gcgcggggcg cgccagcggg tgcggatcgc cgcgctgtcg ccgcggctgc ggcattcgca
     3241 ggccatcgtg gtgggcggcg gcgcgctgct gtcggatgcc aacctgcatt ttccccagtc
     3301 gctggtccgg ctggcgcgcg cggcgcgccg gctcggcaag ccgctggtgt gcctgggctg
     3361 cagcgtcgaa ggcgagtggt cggcgccggg cagccgcatg atcggcgaat tcctcgacgc
     3421 ctgcgaagtc atcgcggtgc gcgatcacgc cacggccggg cgcctcgcgg ccgtcatcgg
     3481 cgcgccgccg ccggtgttcg gggatttttg cctgtgggag tcgcgcgtca tggagggcgg
     3541 cggttccccg caggagcggc attcttatgc gatcaacgtg agccagctgc cgggaccgtg
     3601 ggcgggggca caggaacact atgaagacgc gctggtcgcg ctgatcagcc ggcttgcgcg
     3661 cagggcgcag gggcggcgcg ccggaatccg gatcttcacg accgggacgg ccgaggacgc
     3721 gcgcgccgca cagcgcgtct tcgggcggat cgacgccgtc ggcgcggagc tgcaccttcc
     3781 cggcaatctc gcgcagctct cgtcgatgct gcgcacgagc gcggtggcgg tctcgacccg
     3841 gttgcatgcc gccatcctcg cgctcgccga gcagacgccg gtcgtcggtt tctccgccgg
     3901 cccgaagctg cgcaactact tcgcgactct cggcatccgg cgctgcgcgt acgatgtcgc
     3961 cgaccaggcg cagctcatgg actggctcgc gcaagcggat tacggcgagc tttattccga
     4021 gcagcggcgc gcgctcgtgc actccccgat ctgggcggac cgggcgcggg tgcgccgctc
     4081 gctcgaattg ctcgccgggg cggagaccac gtgcaggtaa tgctgtatct caagcacttt
     4141 cctgcggccg gcgcgccgct gaacgacggg acctccatag ccgtcggcgg gctggcttcc
     4201 ggcctcgcgg aaaacggggc gcgtgtcgcg gtgctgtgcg agggcccggc gcgcagctcg
     4261 attacgaccg agcgcggcta cgcggtcgaa tgtttcgcca accgccatcc gtaccggacc
     4321 ttttcgctcg ctcccggcct gaaacgccat gtcgccgagc atctcgcttc gcgtcccggg
     4381 ctgtgcctgg tgaacggcat gtttcatccg ccggtctacg cgatgggccg gtggctgcgc
     4441 cgcctcggcg tgccctacgt ggtggcgccg cacgatccct acgacagcac cgtgttcagc
     4501 cgcaacgcgc acctgaaatg gccctactgg tacctgttcg agcgcaggct gctcgcgcgg
     4561 gcgagcgcgg tgcaggtgct cgacatccag cacgccgact gcctgcgcgg gctcggcatc
     4621 ggcacgcgcg tgatcgagac gctgaacggc gtcgccccgg gcagcgcccc accggagtcg
     4681 cagctcgcgt ggcgctccgc aagagagccg gtgagcctga tgtttctcgg ccggatcgac
     4741 tcctggaaca aggggctcga catcctgatc gatgcgttcc cgcattttgc cgcgctgacc
     4801 gccgcgcggc tgacggtgca gggaccggac tggggcgacc gcgcccgcct cgaggagctc
     4861 gcggcgcgca ggatgatttc cagcaaggtg gcatttcacg ggccggatta ccggcggccc
     4921 gcgccttgga tcatcgcgga ccacgacgtc ttctgcctgc cttcgcggtt cgaaggcttc
     4981 gggctcgccg cgctcgaggc gatgctggcc gcccgcgtgc tgctggtctc cgagatcgcg
     5041 ggcatcgcgc gccacgtgcg ggcgagcgac tgcggcgtca ctgtcaagcc gacggtggcc
     5101 ggcgtcgcgg agggactggc cgcgctgctg cggcgccggt ccgagtggcg cgaaatggga
     5161 ttgcgcgggc gccgctacgc gctcgccaac ctgcaatgga aaaacatcgc cgccagcgcg
     5221 ctccggcagt acgagcggct ggctgcctga ccccgatgaa cgatcgccag tacgtcgcac
     5281 agtgcctgtt ccagtatctc gatcactgcg aggtggccta ttgcgtggta ggcgacacgc
     5341 gcgactaccc cgaagcgatc cgcagcgaca tcgatatcgt cgtgccgggc tggatgctcg
     5401 acgatttgcc ccgcctcgcc gcccgtttct gccgcgagct cgacattcac ctggtgcagc
     5461 tgatccgtca cgagcagacg gccctctacc tggtcttcgc ctggttcggc gagtcgggcg
     5521 aggcgcgctt tctcgcggtg gatttctgca gcgactattt gcgcggcggc aggctgctgc
     5581 tgggcgcgga agagctgctg gaccaccgcg ggcccgccat cacccggcgc ggtaccaacc
     5641 aggggttccg cgtgccggca ccccacatgc aattcatcta ctacctggtc aagaaggtgg
     5701 acaagcagga tctcgacgac ggccacggcg aatacctgtc gggcgtgtgg gcaatggatc
     5761 cggagggctg ctggagccag ctcaaccgct tctggccggc ggagcgggac gcggaactgc
     5821 tggcggaagc ggccgcgcgc aacgactggt acgaagtgca agccgagctg ccgcggctga
     5881 agcgggcgct gcgccgcgtg gtttccctgt cgcccctgca cgcggcacgc gagctgcggc
     5941 ggtggatcgc gcgcgtgctg cggccgaccg gcctggtggt ggcgttcctg ggcgcggacg
     6001 gcagcggcaa gagcagcgtg atcgagcgcg tgctgacgga cctcgcgccg gctttccggc
     6061 acacccgcta cctgcacctg cgcccgcgcc tgttttcgag ggcggggggc gaggagcggc
     6121 cggtcaccgc gccccatgcg ctccccgcgc gcgggcgcgc cgcatccctc gccaagctcg
     6181 cttattttct tttcgattac ctcttcggcc acgcgtttca cgtgtggccg ctgaagtgcc
     6241 gctccacgct ggtggtgttc gaccgtcatt atcgcgatct cgccattgac gcgcggcgct
     6301 atcgatacgg cggcccgatg aggctggcgc gctgggcggc gaggctcgcg ccgggccccg
     6361 atttctgggt attgctggac gcaccggcgg agatcctgca atcgagaaaa cgggaagtgc
     6421 cggccgagga gtcggagcgc cagcggcacg cttatctcga ggctgcgtcg catttgcatg
     6481 acgctgccgt ggtggacgcg tcgcaaacgc tggagcacgt cgtggcggac accgagtcgg
     6541 ccatactgcg gtggatggag ggccggctcg agttccgcca tcccgagctg cagttcgaga
     6601 ggaatcccgt cacggcgcgc ctgctgcagt atttctgccg caacaagacg ccgctgctcg
     6661 ccaagctgat gcgcatcatc ctgaacagtg acgttcattg ccgcatccat tcgccgatcc
     6721 tgatggcgca tccttacggc atcatcatcc acgccgattc cgtgatcggc cgccgggtgg
     6781 cgatcatgca gcaggtcacg ctgggcggaa aggactgggg tgtgaacgag gcgccggtga
     6841 tcgaggacga cgtctacatc ggggcgggcg cgaaagtcct gggggccgtg cgcgtgggcc
     6901 gcggcgcgat catcggcgcc aacgcggtcg tgacccgcga cgtgccgccg cactgcacgg
     6961 tggtcggggc gaaccgaatc gtgcggcagc cgaaatacgc gagatcggac gcaaacgcgg
     7021 tcgagtttcg cccgccgccc gatgagcgcc tgagcgcgtg aagcgctaac cggtgaaacg
     7081 ggggccatga agccgaccgc attcggaccc gcgaaagagt ttccgtccgc gagcacggac
     7141 ggcgggctgc tctggaccga ttactggtac ctggcggcga ccgcggtcgc ggccgccgtt
     7201 gccgtcgatc cgctcgaatg gggcctggcg tcggaccccg tgatcaagca tcttgcgctt
     7261 gcgatctcgc tgccggcgct cgtgctgacc ttcgcgggta gcaatctttc cgtaaactcg
     7321 cggcgcggag ggccgctggc agtgctgtgg ccgctgctcg cgctcgccat catcgcgctg
     7381 ggcggaagcc tttacgcgcg cgcgatcgag ggcatccaga attcatttct caatatcggc
     7441 ctctacatgc tcacggcgta ctcggccgcg gtgatgatgc tgcgttcgcg ggcgcccgag
     7501 gccctggcgc gcgcgcagct gcgcattctc ctcgccgccg ccgccgtcat ggccgtgtac
     7561 ctcattgcca acttcagggt gcggcaggtg taccacgagc agatatttct cgtgatcccg
     7621 atggcggtgc tgtttttcgc cggcagcctg ccagcgttcg tgcgctgggc ggggtgcctg
     7681 ttctttctcg cgatggcgtg gttttccgcc aagtacacct cctacctgat cggcgcggcg
     7741 acggccgctt acctggtgct gttcgtggcg attccgcgcc tgacgccgcg cagcgggctg
     7801 cggcggacga tgctggccta ctggatctgc gtgctgggag cggccgccgc gctgggcgtc
     7861 gtcctgctcg ggctgcgcgg ctcgttcgag ctgcccaccg gcaacgtcga ctaccggctg
     7921 cacacttaca cggccgcgtg ggagcgcttc ctggattcac cgctgtgggg cacgatgttc
     7981 gcggtcgaag ccgtccagaa attcacgctg tactcgaccg gcgtggcccg caacctgctg
     8041 gcgacccaca gcgacgtgct ggacctgctg gcgaacggcg gcctgctggg catcgtgctg
     8101 tggctttacg ctctcgcgcg catcgcgcgc gccgcattcg cgaacctgct gcggcccggg
     8161 ttatttgagc acgcgtgggc gccgcacgcg cacacgctgg cggtcatgtc gctcgccggc
     8221 atcattacgt acgccttcaa tcccatcctg ctgcagccgg cgctggcgta cctggtgtgg
     8281 accaacctcg ggatgctcgc gggcctggcg ctgcgcatgc aaaccgcgga cgcgccgggc
     8341 ggcggcctgc cgcacccggc cgccgcccgg ccgtggccgg cgcgcggcgc ccatggagtc
     8401 gccggcgcag ggttccatgc gcggtcgcgc acctgatgtc gatcctgctc gaaccggttc
     8461 gggacggcgg ccgcacggcg gcggcgccgc gtgcttcccc cttcagcctg cgcaagctgt
     8521 ggctgttcgt gctggggctg ccgaagacgc tcctgttcaa cttctcgtgc ctgccgttca
     8581 ggcaggcgct gcagctgccc gtgctcgttt cccatcgcgt ttggttgaag gacctttcgg
     8641 gaaaggtcag cctggccgat tgccgcaccg gtgccgtcag gatcgggttc ggcgacgtgg
     8701 gcatattcga cccgcaacgg tcgcgcacga tctggcaggt gccgggacac gtggcgttca
     8761 tgggaacggc cgacatcggc cacggcagca ggatttccgt cacgggagag ctggtgcttg
     8821 gaaaccggct gatcatcgcg gcggagagcg ccatcgtcgc gcagcgcagc atccgcatcg
     8881 gcaatgacgt gctcatttcg tgggacgtgc tgatcatcga caccgacgcg cacccgatct
     8941 acgacgcgac gggaaaccag gtcaatgccg ccatgccgat ccgcatcggg aactcggtct
     9001 ggctggggtg ccgggcgctc gtcctgaagg gggcggagat cgcggacggc gtggtcgtcg
     9061 ccgccgcctc caccgtcacc cgtccggtgc tcacgccgaa tgccatcgtc ggcggcaacc
     9121 cggcgcgcgt tgtcagggag aacgtccggt ggaagcggta atgcccgcat ccgcccgctc
     9181 ggcacccgtc aagatcgcca atagacagga ttaacaggat tcgcaggatt aaatcagaaa
     9241 caaccgcaaa ccatggccga agtttgtcgt ttgaatcctg ttaaggatgc tctgcaaatg
     9301 tcgtttttcg tcattcccgc gaaggcggga atccagggta aacaaatcgt
//
LOCUS       DBNGSG010000126         6251 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3779304, whole genome shotgun sequence.
ACCESSION   DBNGSG010000126 DBNGSG010000000
VERSION     DBNGSG010000126.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6251)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6251)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6251
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3779304"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="AC158Q_03260"
     CDS             complement(<1..642)
                     /locus_tag="AC158Q_03260"
                     /inference="COORDINATES: protein motif:HMM:NF012607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HIR3338816.1"
                     /translation="MSHDVLCLRPEADFTRIGVAPPAQLTIAYRRPDDPQLAALIRDS
                     RALVIPAVGGKIPGSQFEGSQVKLVQVTGAGVDRLDEAAMKKLGIAVANVPGGSNSAV
                     AEYAVGCAIVLLRRLAWSDMEIREANYVSFRGRMVADSLPGLEGLTVGIVGLGTVGFA
                     VAEAFRRFGCEIVYHDPTPRDEAAAKIGARALSLEELLKTADIVSLHVPLLPQT"
     gene            complement(726..1100)
                     /locus_tag="AC158Q_03265"
     CDS             complement(726..1100)
                     /locus_tag="AC158Q_03265"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3338817.1"
                     /translation="MAKVLTQAQAKSMGLPGRKSLEIVSGEKGSQAVTLRLVEIPVPR
                     PGETPRGRHFHNGFEECIYVMSGTGYTESDSGNHPLKAGDTILIPSGEKHATRNTGAE
                     PLVLLCFFPVADITKRTEEQKA"
     gene            complement(1100..1915)
                     /locus_tag="AC158Q_03270"
     CDS             complement(1100..1915)
                     /locus_tag="AC158Q_03270"
                     /EC_number="3.5.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF014670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HIR3338818.1"
                     /translation="MSLKMLPSRYFIDLTQPEIAAQFRKNPLVILPAGSVEQHGPHLP
                     TGTDIYAANVIGHAVAERMDALVLPGGPLGVTPMHMPFEGTITLTPGTYQRVVVETCE
                     STARHGAKRLLILNWHEGNIPSLAIASEKLHRDVGMSVIVVQACYVAAELYGHDCGGL
                     THGGEIEALAVLAYRPDLVHLDRIDYSSDHVHGRKWDKLRRTRSYQPVLRDIRTIAPT
                     GWYGSPEHATVDKGTKMLRDIADAIAREASDIFRLLDEAQGGTAEIKHLKVAS"
     gene            complement(1939..3027)
                     /locus_tag="AC158Q_03275"
     CDS             complement(1939..3027)
                     /locus_tag="AC158Q_03275"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3338819.1"
                     /translation="MSNSPVKVGIVGLGRWAKVLARAAKQSDRLKIVSGDSRSEEKRA
                     AFEKETGVPAAPDMKSMLSNPELKGIILTVPNEQHLPMAEQVARAGKHVYTEKPIAQT
                     LEDGLKIAALEKQHGVTVTVGHSARLMAGIRIIKERIGAGELGRVAFMEANFSNERAL
                     ELTPQTWRWYKDRAPGGPLSQLAIHQFDVLHLLGGEIAEVSSMASKLSPVGAEVDDQS
                     MTTLRFADGKLGYVGSCWTSPGIFAVRVFGSKGLMHYEIDFGTWDTPHLLHKSSVLYI
                     QRGKDGWAKREEIRLPESDMFRAELEMFAESCTSGKPNELTAHNGNVAVAVVYAALRS
                     IDRKGQCVRIADVMEETHARIGSQARAA"
     gene            complement(3169..4152)
                     /locus_tag="AC158Q_03280"
     CDS             complement(3169..4152)
                     /locus_tag="AC158Q_03280"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3338820.1"
                     /translation="MKLRSLLSLPLAVALLAPAAPATAQEAAKNYPSRPIRLLVPNAP
                     GSAVDTLSRIVGNRLSEAVGQQVVMDNRAGAGGIIGMEIAKSANPDGYTVISATTAAT
                     TIARLLQKSATFHPVNDYDYVVQYAETLNVLVVNPSLPIKSVKELIAYGKSGKKPFNM
                     ASAGAGSQSHLSGAYFVQAAKFESLHVPYKGGGPSSAAVVAGESQWTLIPAPAAMTHV
                     NAGRMRALAHTLPRPTALLPNIPPISDTLPGFDYSGWMGFFVPKGTPRPIIDKLRTAV
                     VKTMQHPDVKKGMAFQATEIVIRGPAEFRKVVQASMVENEKLVKSLGLAAN"
     gene            4328..5257
                     /locus_tag="AC158Q_03285"
     CDS             4328..5257
                     /locus_tag="AC158Q_03285"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR3338821.1"
                     /translation="MTVRITAALAAVLIAACTLLPTNHNGAGAPRQAAVIPLKSDRPL
                     VALALGSGGARGFAHVGVIKALEEAGIVPDIVTGSSSGAVVAALYADGRGARELERIA
                     LEVEPSDLVDFVLFGKGWVKGKALQEFVNRMVDGKPIERLAKPFAVVATEAKSGRMTV
                     FNRGGTGLAVRASASVPNVFVSPVINGEEYVDGGLSSPVPVKLARAMGADIVIAVDVS
                     WFAQARNADSAGAMAQHGRGGRYVLLAGELDAADVVIAPRTARTRMLDFGQKETNIAA
                     GEAAGREAVLQLREMIARTAAAKQARLHTAGKQ"
     gene            5476..6039
                     /locus_tag="AC158Q_03290"
     CDS             5476..6039
                     /locus_tag="AC158Q_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR3338822.1"
                     /translation="MRSSLVRGVFSVWIILSFCAAQALAQDGAKPEAYAEETRDWGVP
                     PTRVLRTVGYHSPTPLRIPGGKTVTTLELKALLEGDPRPYLIDVLGGGVHRTIAGAFW
                     MIGAGAGDMSRDEESRFAKALAAFAGGDKSRPMVFFCVDPECWLSYNTALRAIALGYT
                     NVMWYRGGVAAWRSAELPMTQSDPFFW"
BASE COUNT         1024 a         1975 c         2067 g         1185 t
ORIGIN      
        1 ggtctgcggc agcagcggca catgcagaga gacgatgtcg gcggtcttga gcagttcctc
       61 gagcgacaag gccctcgccc cgatcttcgc ggcggcttcg tcgcgcggcg tcgggtcgtg
      121 atagacgatc tcgcagccga agcggcggaa tgcctcggcc accgcaaacc cgaccgtgcc
      181 caggcccacg atgcccaccg tcagtccttc cagaccgggc agactgtcgg cgaccatgcg
      241 cccgcggaac gagacataat tggcctcgcg gatctccatg tcggaccagg ccagccgccg
      301 caacagcacg atggcgcagc cgaccgcata ctcggcaacc gcgctgttgc tgccgccggg
      361 gacattggca accgcgatgc ccagtttctt catcgccgct tcgtcgagac ggtccacgcc
      421 ggcaccggtc acctgcacca gcttcacctg gctgccctcg aactgcgaac ccggaatctt
      481 tccacccacc gccgggatta cgagcgcgcg cgagtccctg atcagggcgg cgagctgcgg
      541 gtcgtcgggc ctgcggtagg caatcgtcaa ctgcgcgggt ggcgcaacgc cgatgcgcgt
      601 gaaatcggct tcgggacgga ggcacaggac gtcatgagac atagattcgt gattcgcgat
      661 tggtgaagga aagacccggt caggggataa aggtggtgtt cgactcacga ttcatgaatc
      721 accactcacg ccttttgctc ttcagtgcgt ttcgtgatgt cggccaccgg gaagaagcag
      781 agcagcacca gcggctcggc tccggtattg cgggtcgcgt gcttttcccc tgatgggatc
      841 agaatggtgt cgcccgcctt gagggggtgg ttgccgctgt cggactcggt atagcccgtg
      901 cccgacatca cgtagatgca ctcctcgaag ccgttgtgga aatggcggcc gcgcggcgtc
      961 tccccgggtc tcggcaccgg gatctcgacg agccgcagcg tcaccgcctg cgaccctttc
     1021 tccccggaca cgatctcgag cgacttgcgc ccgggcaggc ccatgctctt ggcctgcgcc
     1081 tgggtcagga ctttcgccat cagctcgcta ccttgaggtg cttgatctcg gcggtgccgc
     1141 cttgcgcctc atccagcagg cggaagatgt cggatgcctc cctcgcgatc gcgtccgcga
     1201 tgtccctcaa catcttcgtg cccttgtcta ccgtcgcgtg ctcggggctg ccgtaccagc
     1261 cggtcggggc gatggtcctg atatcgcgca gcaccggctg gtagctgcgg gtgcggcgca
     1321 gcttgtccca cttgcggccg tgcacgtgat cggaggagta atcgatacga tcgaggtgca
     1381 cgaggtcggg acggtaggcg agcaccgcca gcgcctcgat ctcgccgccg tgcgtaaggc
     1441 cgccgcaatc atgtccatag agctcggccg cgacgtagca cgcctgcacc acgatgacgc
     1501 tcatgcccac gtcgcgatgc aatttctcgg acgcgatggc gagcgagggt atgttgcctt
     1561 cgtgccagtt gaggatcagc aggcgcttcg cgccgtgcct tgcggtcgat tcgcaggttt
     1621 ccaccaccac gcgctggtag gtcccgggcg tcagcgtaat ggtcccctcg aacggcatgt
     1681 gcatgggggt cacgcccagc ggcccgcccg gcagcaccag ggcgtccatg cgctcggcca
     1741 cggcgtgccc gatcacattg gcggcgtaga tgtcggtgcc ggtcggcagg tgcggtccgt
     1801 gctgctcgac gctgcccgcc ggcaggatca ccagcgggtt tttcctgaac tgggccgcga
     1861 tttccggctg ggtgaggtcg atgaagtagc ggctgggcag cattttgagt gacaagtgaa
     1921 gggtggcggg tgacgaagtc aggcggcacg cgcctggctg ccgattcttg cgtgcgtctc
     1981 ttccatgacg tcggcgatgc gcacgcactg tcccttgcgg tcgatcgatc tcaatgctgc
     2041 gtaaaccacg gctactgcga cgttgccatt gtgggcggtg agttcgttcg gcttgccgct
     2101 cgtgcagctc tccgcgaaca tctcgagttc ggcgcggaac atgtcgcttt cggggagccg
     2161 gatctcctcg cgcttcgccc aaccgtcctt gccgcgctgg atgtagagga cggacgactt
     2221 gtgcaggaga tgcggcgtgt cccaggtgcc gaaatcgatc tcgtagtgca tcagcccctt
     2281 cgagccgaac acgcggaccg cgaagatgcc gggcgacgtc cagcacgagc cgacgtaacc
     2341 cagcttgccg tcggcaaacc ggagcgtcgt catcgactgg tcgtccacct ccgcgccgac
     2401 cggcgagagc ttggaagcca tcgaggaaac ctcggcgatc tcgcccccca gcaggtgcag
     2461 cacgtcgaac tggtggatcg cgagctggga gagcgggccg ccgggcgcgc ggtccttgta
     2521 ccagcgccag gtctggggcg tcagctccag cgcgcgttcg ttggagaaat tggcttccat
     2581 gaaggccacg cgccccagct cgccggcgcc gattctctcc ttgatgatgc ggatgccggc
     2641 catcagccgg gcgctgtggc cgacggtaac ggtgacgccg tgctgctttt cgagggcggc
     2701 gatcttcaat ccgtcttcga gggtctgtgc gatcggcttc tcggtgtaga cgtgcttgcc
     2761 ggctcgcgca acctgttccg ccatcggcag atgctgctcg ttgggcacgg tcaggatgat
     2821 ccccttcaac tccgggttgg agagcatgga tttcatgtcc ggcgccgccg gcacgcccgt
     2881 ttccttttcg aacgcggcgc gtttttcctc ggagcggctg tcgccggaaa cgattttcag
     2941 ccggtcggac tgcttcgcgg cgcgggccag caccttggcc cagcggccga gaccgacgat
     3001 gccgactttc acgggggaat tgctcatggg atgtccggtt ttcctgggaa gtgcgtcatt
     3061 ctacaacgcg gtgggccggc gccacctgcg gtgcggccca aaaaacaggg gcggccgcgg
     3121 ccgcccctga atacctgtca acggtcactg gtccatcgtc acgggttcct agttggcggc
     3181 caacccgagg gatttgacga gcttttcgtt ctcgaccatc gaagcctgca ccactttccg
     3241 gaattccgcc ggcccccgga tcacgatctc ggtggcctgg aaggccatgc ctttcttgac
     3301 atccggatgc tgcatggtct tcaccactgc cgtgcgcagc ttgtcgatga tcggcctggg
     3361 agtgcctttc ggaacgaaga atcccatcca gccgctgtaa tcgaaaccgg gcagggtatc
     3421 cgagatcggc gggatattgg ggagcagcgc ggtaggccgg ggcagggtgt gcgccagcgc
     3481 gcgcatgcgg cccgcgttca catgggtcat tgccgccggg gcgggtatca gggtccactg
     3541 cgattccccc gccaccaccg ccgcactgga agggccaccg cccttgtacg gcacgtgcag
     3601 ggactcgaac ttggcggcct ggacgaaata cgctccggag aggtggcttt gcgagccggc
     3661 gcccgccgag gccatgttga acggtttctt tccgctcttg ccgtaagcga tgagttcctt
     3721 caccgacttg atcggcagcg acgggttgac cacgagcacg ttgagggttt ccgcgtattg
     3781 cacgacataa tcgtagtcgt tcacggggtg gaaggtggcg ctcttttgca gcaggcgggc
     3841 gatggtggtc gccgcggtcg tggcgctgat caccgtatag ccgtccgggt tcgcggactt
     3901 cgcaatctcc atgccgatga tgccgcccgc gcccgcgcgg ttgtccatca cgacctgttg
     3961 cccgaccgct tcggacagcc tgttgcccac gatgcggctc aacgtgtcca ccgcgcttcc
     4021 cggcgcgttc ggcaccagca atcggatcgg ccggctggga tagttcttgg cggcctcctg
     4081 ggcggttgcc ggggccgcgg gcgcgagcag cgcgacggcc agaggtagcg aaagcaagct
     4141 gcggagcttc atgaattcct ccttatttat taggacccgg tgcgcgaagg cagggggcgt
     4201 ttccaggcta attctgtcgg gtttgtccaa tgaacgcaac tgacctgccc gccgccgttt
     4261 tccgggaacc ccggggcggc ccggcgctat cgttgtgggt gcgcatttcc attacaatgc
     4321 cgctcccatg accgtcagaa tcaccgccgc tctcgccgcc gtcctgattg cggcctgcac
     4381 cctgctgccg accaatcaca atggcgccgg cgcaccgcgc caggcggcgg ttatcccgct
     4441 caaatcggac cggccgctcg tcgcgctcgc gctcggcagc ggcggcgccc gcggctttgc
     4501 ccatgtcggc gtgatcaagg cgctggagga agcggggatc gttcccgaca tcgtcacggg
     4561 atccagttcc ggggccgtgg tcgccgcgct ctatgccgac ggtcgaggcg cgcgcgagct
     4621 ggagcggatt gccctcgagg tcgagccaag cgacctggtg gacttcgtgc tgttcggaaa
     4681 gggatgggtc aagggcaaag cgctgcagga attcgtcaac cgcatggtgg acggaaagcc
     4741 gatcgagcgg ctggcgaaac ccttcgcggt tgtcgcgacc gaggcgaaga gcggccgcat
     4801 gacggtattc aaccgcggcg gtaccgggct tgccgtgcgc gcttcggcga gcgttcccaa
     4861 cgttttcgtt tctcccgtca tcaacggcga ggaatacgtg gacggcgggc tctcgagccc
     4921 ggtgccggtg aagctggcgc gtgcgatggg cgccgatatc gtgatcgccg tggacgtttc
     4981 ctggtttgcg caggcgcgca acgccgattc ggcaggcgcg atggcgcagc acggccgcgg
     5041 cgggcgctat gtactgctcg ctggcgagct cgacgcggcc gacgttgtca tcgcgccgcg
     5101 gaccgcgcgc acgcgcatgc tcgatttcgg gcaaaaggaa accaacatcg cggccgggga
     5161 ggcagcgggc cgcgaggcgg tactgcagtt gcgcgaaatg atcgcaagga ctgccgccgc
     5221 caagcaggcg cgcctgcaca ctgctggaaa gcaatagaca ggattaactg atccgaaaac
     5281 ccgtcaaacc cgttaataga caggattaac aagattaacg ggattcaatc aagctcaaga
     5341 atcgaacggg atgttgcttt aattctgtaa atcctgttaa tcctgtccat ttgtggtttt
     5401 agcgggtttg caattggcac gtaagtcctg tccattcgag ttttcagcga acacagcatc
     5461 aggagaaagt gggtcatgag gagcagtttg gttcggggcg tgttcagtgt ctggatcatc
     5521 ctgtcttttt gcgcagcgca agccctggcg caggacgggg cgaagccgga ggcctacgct
     5581 gaggaaactc gcgattgggg cgtgccgccg acccgggtat tgcgcacggt cggctaccat
     5641 tcgccgacgc cgctgcgaat acccggcggg aagaccgtca ccaccctgga gctcaaagcc
     5701 ttgctggagg gcgatccgcg gccgtacttg atcgacgtgc tgggaggcgg cgtccaccgc
     5761 accatcgcgg gcgcgttctg gatgatcggt gcgggcgccg gcgacatgag cagggacgag
     5821 gaatcgcgct tcgcgaaggc ccttgccgct ttcgccggcg gcgacaagag ccgtcccatg
     5881 gtgttcttct gcgtggaccc ggagtgctgg ctgtcctaca acactgcgct gcgcgccatc
     5941 gcgctcggct acaccaacgt catgtggtac cgcggcggcg tcgcggcatg gcgcagcgcg
     6001 gagctgccga tgacgcagtc cgacccgttt ttctggtagg gatgagggcg gagggatgag
     6061 ggataaggac ccggccgccc gggttgggtt gaaagcgctt tttcgcaact tcctcatccc
     6121 tcacaaatca tccctcatcc ctgagttggc gcgggcgcgg acgcaacgcg tggcacgcgc
     6181 gccaaatccc agttcttgat tgcccagtcc cagaatatcc tgatcgttcc ccgcgccaat
     6241 tcgtcaggcg g
//
LOCUS       DBNGSG010000127         7866 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2251405, whole genome shotgun sequence.
ACCESSION   DBNGSG010000127 DBNGSG010000000
VERSION     DBNGSG010000127.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7866)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7866)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7866
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2251405"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="AC158Q_03295"
     CDS             <1..613
                     /locus_tag="AC158Q_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GltB/FmdC/FwdC-like GXGXG domain-containing
                     protein"
                     /protein_id="HIR3338823.1"
                     /translation="TNDYCGKGLSGGRISVQPSPKFRGEPTENIITGNVVLYGAICGE
                     AYFRGVAGERFAVRNSGAQAVVEGVGDHGCEYMTGGTIVVLGVTGRNFAAGMSGGIAY
                     VLDVDGEFARRCNTAMVALEPLLAETEQDAKLARELWHGAMADEIVVKRLVENHARYT
                     ASAQARRILENWSGYRTKFVKVFPHEYRRALGELAAKRRRLVA"
     gene            714..2177
                     /locus_tag="AC158Q_03300"
     CDS             714..2177
                     /locus_tag="AC158Q_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003271421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HIR3338824.1"
                     /translation="MGKITGFLEFDRVEEAYEPKEQRKKHYREFIPPLSDEQAQTQGA
                     RCMDCGIPFCMSGCPVNNIIPDWNDLVYRQDWKAAIDTLHSTNNFPEFTGRICPAPCE
                     EACVLRINEDAVGIKSIEHAIIDKAWERGWVKPQPAARKTGKKVAVVGSGPGGLACAQ
                     QLARAGHDVIVFEKNDRIGGLLRYGIPDFKLEKAQIDRRMEQMVAEGVLFRTGVYVGN
                     QRPGKGVPDWATGTIAPKKVLAEFGAVVLAGGAEQPRDLPIPGRELDGVHFAMEFLPL
                     QNKRVAGDAGVPDLWATGKHVVIIGGGDTGSDCVGTSNRHGAQSVTQFELLPMPPDVE
                     RNPVWPYWPTRLRTSSSHEEGVQRDWSIATKEFVGEGGRVKALRAVRLAWKNGRMSEV
                     PGSEFTLPADLVLLAMGFVSPVQSILESFGVDKDARGNAKATTDGQGCFSTSVPKVFA
                     AGDMRRGQSLVVWAIREGRQCARAVDEFLMGSSELPR"
     gene            2263..3324
                     /gene="hemE"
                     /locus_tag="AC158Q_03305"
     CDS             2263..3324
                     /gene="hemE"
                     /locus_tag="AC158Q_03305"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HIR3338825.1"
                     /translation="MAKLKNDTLIRALLRQPTSHTPVWLMRQAGRYLPEYNQTRARAG
                     SFLALAKNPDLAAEVTVQPLARFRLDAAIVFSDILTIPDAMGLGLAFAEGEGPYFERP
                     LREEREIRALVVPDPATRLRYVLDTVSQVRRALANEIPLIGFSGSPFTLACYMVEGRG
                     SADFRLIKSLLYNRPELLHHILEINCQAVSAYLNAQIEAGAQAVMLFDTWGGVLGPVE
                     YREFSLAYMQRVLETLKREHDGERIPCILFTKGGGMWLEAIAASGCDAVGLDWTVDIT
                     AARRRVGKRVALQGNLDPAALLASPEAARRGAAAVLAAFGRGSGHVFNLGHGVSQFTA
                     PENVVALVEAIHELSPAYH"
     gene            3658..5847
                     /locus_tag="AC158Q_03310"
     CDS             3658..5847
                     /locus_tag="AC158Q_03310"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HIR3338826.1"
                     /translation="MPIIRVAIDVPVDTLFDYRILDATAADIGRRVQVPFGNKTATGV
                     IIEVSSASEVPEARLKNALRVLRDMPPLAPEDLRLIRFAADYYCHPLGAVVMDTLPSR
                     LRKVAAGSSRPARRGVYLLTAAGTALAAAALPGRAHARLRLLARLRSGPLGTAEAKAL
                     SSSAPSLLKQFIARGWVKQEEDSGTDKPPLPPAATSPPVLSPEQAIAVAEIRAGLGRY
                     QPFLLLGVTGSGKTEVYLHAIGATLDAGRQALLLVPEIALTPQLEALVALRYPRTRTV
                     SLHSGLAESERLESWRAARDGQARIILGTRLAVFAPLPDLGLIVVDEEQDGSFKQSEG
                     FRYSARDLAVTRARQRDVPIVLGTATPAFETYHNAVTGRYTLLELSSRIGAPAPHIVC
                     VDTRNEKLADGLSRRLLDALRSRIAAGEQSLIFVNRRGYAPVLMCRACGWTSGCRRCA
                     ALLVLHLAARRLRCHHCGHDSPIPVACPDCGNPDLAPVGQGTQRVEEALARHFPQARI
                     LRIDRDTTSRRSAWQAMRRRIQDREVDVLVGTQILAKGHDFPHLNLVGVLNADSRLYS
                     SDFRAAEQLYALLTQVAGRAGRSGVQGEVLIQTEFPHHPLYAALCRQDYRAFADGALA
                     ERKQAGFPPFVYQALLRAEAPQLATALAFLAQAARKGKGLDAGVTIYDPVPAALPRRA
                     GRERAQLLVQSESRQQLRTFLAAWRGGLSDLRATRARWSLDVDPLDF"
     gene            5924..7669
                     /gene="argS"
                     /locus_tag="AC158Q_03315"
     CDS             5924..7669
                     /gene="argS"
                     /locus_tag="AC158Q_03315"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HIR3338827.1"
                     /translation="MTTALPGDARAHLAGLLAHALHAIAPGHTAAAIVLERPKHAGHG
                     DYACSVALQLAKLLRARPRDIAAQLVSALPHSPCLEKAEVAGAGFINLFLKRSFKQLA
                     VNRVLQAGSAYGALALGRNKRVQLEFVSANPTGPLHVGHGRGAAYGASLANVLVAAGF
                     DVTREYYVNDAGRQMDILVLSTWLRYLELFGSAVAFPGNAYQGEYVHRMAQAIRARHG
                     EQFRHGAAAGDFHSVLAEPDEEVRLDKLIAFARQRLGGDYAVLQGFVLSEQLEDCRSD
                     LAEFGVTFDNWFSEQSLYDSGRVERAVTMLEEKGCIFSEDGARWFRSTDFGDEKNRVV
                     QRENGQYTYFASDIAYHQYKFEQGFELVIDVWGADHHGYIARVKGALAALGLNSGQLT
                     IALVQFAVLYRGGQKVSMSTRSGEFVTLRELRREVGNDAARFFYVLRKSDQHLDFDLD
                     LAKTQSNENPVYYVQYAHARVCSLFEQWGGDAAGLSDADPAALVSEHELALLARLMEY
                     PEAVENAACDLSPHIVAFYLRELAAAFHSYYNATRILVDEENVQLARLALAAAVRQVL
                     RNGLALLGVSAPEKM"
     gene            7680..>7866
                     /locus_tag="AC158Q_03320"
     CDS             7680..>7866
                     /locus_tag="AC158Q_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338828.1"
                     /translation="MSKDYKRPEPKSRSSGSSLVIGILIGLVLGLGIALAVAWYINKM
                     PSPFLSRPPAVKGDPPKD"
BASE COUNT         1353 a         2534 c         2544 g         1435 t
ORIGIN      
        1 caccaacgac tactgcggca agggcctgtc aggcgggcgt atctcggtac agccctcgcc
       61 caagtttcgc ggagagccca ccgagaacat catcaccggc aacgtcgtgc tctacggtgc
      121 gatctgcggc gaagcttact tccggggcgt tgccggcgag cgttttgcgg tgcgcaattc
      181 gggtgcccag gccgtggtcg agggcgtcgg ggaccacggt tgcgagtaca tgacgggtgg
      241 caccatcgtg gtgctgggcg tgactggtcg caatttcgcc gctggcatgt cgggcggcat
      301 cgcctatgtg ctcgacgtgg acggcgagtt cgccaggcgc tgcaacactg caatggtggc
      361 gctggagccc ttgctcgccg agacggaaca ggacgcaaaa cttgctcgcg agctgtggca
      421 tggcgcaatg gccgacgaga tcgtcgtcaa gcggcttgtc gagaatcacg cccgctacac
      481 cgcgagcgcc caggcaaggc gaatactcga aaactggagc gggtatcgga cgaaattcgt
      541 caaggtgttc ccgcacgagt atcggcgcgc gctgggcgag cttgcggcca agcgccggcg
      601 cctcgtcgca tagccggtaa caacggtttc ggtccctggt gccggagacg gcggggcctc
      661 ccgaaccctt cggatcccgg gcagccagtc aacagccacg catcatagaa acgatgggaa
      721 aaatcaccgg atttcttgag tttgatcggg tcgaagaagc ttacgagccg aaggaacagc
      781 gcaagaagca ttatcgggag ttcatcccgc ccctgtccga cgagcaggcg cagactcagg
      841 gcgcgcgctg catggattgc ggcattccgt tctgcatgag cggctgcccg gtcaacaaca
      901 tcatcccgga ctggaacgat ctggtttacc ggcaggactg gaaagcggcg atcgacacgc
      961 tgcactccac caacaatttt cccgagttca ccggccgcat ctgtcccgcg ccttgcgagg
     1021 aggcgtgcgt tttgcgcatc aacgaggatg cggtaggcat caagtcgatc gagcacgcga
     1081 tcatcgacaa ggcgtgggag cggggctggg tcaagccgca accggctgcc cgaaaaaccg
     1141 ggaaaaaagt ggcggtggtg ggctcaggcc cgggagggct cgcctgcgct cagcagctgg
     1201 cgcgtgcagg ccacgacgtg atcgtgttcg agaagaacga tcgcattggc ggactgttgc
     1261 gctacggaat ccccgatttc aagctcgaaa aagcgcagat cgaccgacgc atggaacaga
     1321 tggtcgccga aggcgtgttg tttcgcaccg gtgtctacgt cggcaatcag cgccccggca
     1381 agggcgttcc cgattgggcg accgggacca tcgcgccgaa aaaggtgctc gcggagtttg
     1441 gcgcggtggt gttggccggc ggtgcggaac agccgcgcga cctgccgatt cccggtcggg
     1501 aactcgacgg cgtgcacttt gcgatggaat tcctgccgct gcagaacaag cgtgttgcgg
     1561 gcgacgccgg cgtccccgat ctgtgggcga ccggcaagca cgtcgtgatc atcggtggcg
     1621 gtgacaccgg ctctgattgc gtcggtactt cgaaccggca cggcgcccag tccgtcaccc
     1681 agttcgagtt gctgccgatg ccccccgacg tggagcgaaa tccggtatgg ccgtactggc
     1741 cgacgcggct gcgcacttcg tcctcgcacg aggaaggggt gcagcgcgat tggtcaatcg
     1801 ccaccaagga attcgtcggc gagggcggca gggtcaaggc gctcagggcg gtgcggctcg
     1861 cgtggaagaa cggcaggatg agcgaggttc cgggctcgga gtttacgctg cctgccgatc
     1921 tcgtgctgct cgcgatgggc ttcgtttcac ccgtgcaatc catcctggag tctttcggcg
     1981 tggacaagga cgcccgcggc aacgccaagg cgactacgga tgggcagggc tgcttctcga
     2041 cctcggtgcc caaggttttt gccgccggcg acatgcggcg cggacagtcg ctggtggtct
     2101 gggcgattcg ggaaggccgc cagtgcgcgc gcgcggtgga cgagttcctc atgggttctt
     2161 cggaattgcc gcgctagacg gcaaatcccg acgaccccgg cgcaggaaca ggctcgcggc
     2221 accccgcgcc ccctgctgcg gagtcggggt gcatcaatca atatggccaa actcaagaat
     2281 gacacgctga tccgggcact gctgcgacaa cccacctccc ataccccggt atggctgatg
     2341 cgccaggccg gccgctacct gcccgaatac aaccaaaccc gggcgcgcgc cggcagcttc
     2401 ctcgcgcttg ccaagaatcc cgatctcgcg gccgaggtga cggtgcagcc gctcgcccgc
     2461 ttccgcctcg acgccgccat cgttttttcc gacatcctga cgatccccga cgcaatgggg
     2521 ctggggctgg ctttcgccga aggcgaaggg ccttacttcg agcgtcccct gcgggaggaa
     2581 cgcgagatcc gtgcgctcgt ggtccccgat cccgccacgc ggttgcgcta tgtactcgat
     2641 accgtcagcc aggtgcggcg agcgcttgcc aatgagatcc cgttgatcgg cttctccgga
     2701 agccccttca cccttgcctg ctacatggtg gaggggcgcg ggagcgctga ttttcgcctg
     2761 atcaagtccc tgctctacaa ccgtcccgaa ctgttgcacc acatactgga aatcaattgc
     2821 caggcggtct ccgcctatct caacgcgcag atcgaggcag gcgcccaggc ggtgatgctg
     2881 ttcgacacct ggggtggcgt tctcggtccc gtcgagtacc gggagttctc gctcgcctac
     2941 atgcagcgtg tactcgaaac gctcaagcgc gaacatgacg gcgaacgtat tccgtgcata
     3001 ctgtttacca agggcggggg tatgtggctc gaggccattg cggcgagcgg ctgcgacgcg
     3061 gtcggcctcg actggacagt ggatatcacc gcggcacgcc gtcgcgtcgg caagcgcgtc
     3121 gcgctgcagg gtaacctcga ccccgctgcg ctgctggcaa gccccgaagc ggcgcgccgt
     3181 ggcgctgccg ctgtgctcgc tgcgtttggc cgcggctccg gccatgtatt caacctgggg
     3241 cacggcgttt cgcaattcac ggcacctgaa aatgtcgtgg cgctggtgga agcaatccac
     3301 gaactgagcc cggcttatca ctgaagatct gcgccagttc tgactttgca ggcgacggcc
     3361 gcgaagttat gcacagcaac acgttgtgtc aaaagggatt tttatcggac acctgcctgt
     3421 accgggtttc gccataacgt attgttttat atgcattaat agggctacct taggggtgat
     3481 ggcctctggc ttgggtcggc tggccagggc cggggtcgga aatcctatca acatggttat
     3541 ccacacccgc gccatgttga taatctcggt gtccgcggcc ctgccacaaa actcgctcgc
     3601 gcattaacag agccgctctc cgcaagccag gcactttcct cccacgtcac tgccgagatg
     3661 cccataatcc gcgtcgcgat tgatgtgccg gtcgacacct tgttcgacta ccggatcctc
     3721 gacgcaacgg cggcagacat cggccgccgc gtgcaggtgc cgttcgggaa caaaaccgcg
     3781 accggcgtga tcatcgaggt atcgagcgcc tccgaggttc cggaagcgcg actgaagaat
     3841 gcgttgcgcg tgttgcggga catgccgccg ctggcgcccg aggatctgcg cctgatccgg
     3901 ttcgcggccg attactactg ccatcccctt ggcgcggtcg taatggacac cttgccgtcc
     3961 cggctgcgca aggtggctgc cgggtcatcg cgcccagcaa gacgtggcgt ctatctcctc
     4021 acggcggccg ggacagcgct cgcggcggct gcgctgcctg gacgtgccca tgcacggctg
     4081 cggttgcttg cgcgactgcg gtcaggtcca ctgggcacgg ccgaggcgaa agcgctttcc
     4141 tccagcgcgc catccctgct gaaacagttc atcgcgcgcg gctgggtcaa gcaggaagag
     4201 gattccggca cggataagcc acccttgccg cccgccgcga cttcgccgcc cgtgctaagc
     4261 cccgagcagg cgattgcggt cgctgaaatc cgcgccggtc ttggcaggta ccagccgttt
     4321 ctgttgctgg gggtcaccgg cagcggcaag accgaggttt atctgcatgc catcggcgcc
     4381 acgctcgacg cgggaaggca ggcattattg ttggtaccgg agatcgcact gacgccgcaa
     4441 ctggaagcgc tggtggcact tcgctacccg cgtacccgca cggtcagcct gcacagcggt
     4501 cttgctgaat cggagcgtct ggagagctgg cgggcggccc gcgacggcca ggcccgcatc
     4561 atcctcggca cgcggctggc ggtgtttgcg ccgctgccgg atctcggcct gatcgtcgtg
     4621 gacgaagagc aggacggctc tttcaagcaa tccgagggtt ttcgctattc ggcgcgcgat
     4681 ctcgccgtaa cgagagcacg gcagcgggac gtgcccattg tgctggggac tgcaaccccg
     4741 gcgttcgaga cctaccacaa cgcggtcacc gggcgttata cgctgctcga gctatcgagc
     4801 cgcatcggcg cgccggctcc gcacatagtc tgcgtcgata cccgcaacga gaaactcgcg
     4861 gacgggttgt cgcgacgttt gctggacgcc ttgcgcagcc gcattgccgc tggcgagcaa
     4921 tccctgatat tcgtcaaccg ccgcggctac gccccggtgc tgatgtgccg ggcttgtggc
     4981 tggacctcgg gctgccgccg ttgtgcggcg ctgctggtgc tgcacctggc tgcgcggcgc
     5041 ttgcgctgcc accattgcgg ccacgactcg ccgataccgg ttgcctgccc cgattgcggc
     5101 aatcccgatc tcgctccggt ggggcagggc acgcagcgtg tggaagaagc gctggcgcgt
     5161 cattttcccc aggcgcgcat cctgcgcatc gaccgcgata ccacgagccg ccgcagcgcc
     5221 tggcaggcga tgcgccggcg catccaggac cgggaggtcg atgtcctggt tggcacgcag
     5281 atacttgcca agggccacga tttcccgcac ctgaacctgg tcggggtgtt gaacgccgat
     5341 agccggctct acagcagcga ttttcgcgcc gcggagcagc tgtacgccct gttgacccag
     5401 gtcgcgggac gtgccggccg cagcggggta cagggagaag tgctgatcca gaccgagttt
     5461 ccgcatcatc cgctctatgc cgccctgtgc cgccaggact accgcgcctt cgcggacggc
     5521 gccctcgcgg agcgcaagca ggcaggcttt ccgcctttcg tctaccaggc tctgctgcgg
     5581 gccgaggcgc cgcagctcgc cacggcgctg gcgttcctgg cgcaggcagc gcgcaagggc
     5641 aaggggctcg acgccggcgt gacgatctac gacccggtgc cggccgcgct gccgcggcgg
     5701 gccggacgcg agcgcgcgca attgctggtg cagtcggaat cgcggcaaca gttgagaact
     5761 tttcttgcgg cctggcgtgg cgggctatcc gatctccgcg ctacccgcgc acgctggtcg
     5821 ctcgacgttg atccgctcga tttttgaccg ggcgattgga atccgtgccg tgataaaatt
     5881 cgctctcccc gcacccagct tccccgctta gcacggacat ggcatgacca cagcgctccc
     5941 tggcgacgcg cgagcgcacc ttgcgggcct gcttgcgcat gcccttcacg ccatcgctcc
     6001 gggccacacg gctgccgcca tcgtgctcga gcgccccaag cacgccgggc acggtgacta
     6061 tgcctgcagt gtggcgctgc agctcgcaaa attgctgcgc gccaggccgc gcgacatcgc
     6121 tgcgcaactg gtttcggcac tcccgcactc accgtgcctg gaaaaggctg aagtcgcggg
     6181 agcgggattc atcaacctgt tcctgaagcg ctccttcaag cagttggcgg tcaaccgcgt
     6241 actgcaggcg ggctcagctt acggcgcact cgcgctcggc cgtaacaaac gggtgcagct
     6301 cgagttcgta tcggccaatc ccaccggacc gttgcacgtc ggccatggac gcggcgcggc
     6361 gtatggggcg agccttgcca acgtgcttgt cgctgcgggc tttgatgtca cccgcgagta
     6421 ctacgtcaac gacgccggcc ggcagatgga catcctcgtg ctatccacgt ggctgcgcta
     6481 tctcgagctg ttcggcagcg cggtcgcctt tccgggcaac gcctatcagg gcgagtatgt
     6541 tcacaggatg gctcaggcaa tccgggcccg ccacggcgag caatttcgcc acggagccgc
     6601 ggcgggtgac ttccattccg tcctggccga gccggatgaa gaagtaaggc tcgacaagct
     6661 gatcgcattc gcgcgccagc gcctcggagg ggattacgcc gtgctccagg gtttcgttct
     6721 cagcgaacag ctcgaggatt gccgttccga cctggcggag ttcggagtca cgttcgataa
     6781 ctggttttcc gagcaatcgc tgtacgactc ggggcgggtc gagcgtgccg tcacaatgct
     6841 cgaagaaaaa ggctgcatct tctcggaaga cggcgcccgc tggtttcgct cgaccgactt
     6901 cggcgatgaa aagaaccgcg tggtccagcg cgagaacggc caatatacct atttcgcttc
     6961 ggacatcgcc taccatcaat acaagttcga gcaaggattc gagctcgtga tcgatgtatg
     7021 gggcgccgat catcacggct acatagcgcg tgtcaagggc gcgctcgcgg ccttgggcct
     7081 taattccggg cagctcacca tcgccctggt gcagtttgcc gtgctctacc gcggcggcca
     7141 gaaagtctcc atgtccaccc gttccggcga gttcgtcacg ctgcgcgagt tgcggcgcga
     7201 ggtcggcaac gacgcggcgc gtttcttcta cgtgctgcgc aagagcgacc agcatctcga
     7261 ttttgacctc gatctggcca agactcagag caacgagaat cccgtctact atgtccagta
     7321 tgcgcacgcc cgtgtgtgca gcctgttcga acagtggggt ggcgacgcag cgggcctctc
     7381 cgatgccgat ccggccgccc tcgtatcgga gcacgaactc gccctgcttg cgcgcctgat
     7441 ggaatacccg gaggcagtgg aaaacgccgc gtgtgacctg tcgccgcata tcgtcgcgtt
     7501 ctatctgcgg gaactcgccg cggcgttcca cagctactac aatgcgaccc gcatactggt
     7561 ggatgaagaa aacgtccagc ttgcgcgtct cgcgctcgct gccgcggtac gccaggtgct
     7621 gcgcaacggg ctcgcgctgc tgggtgtctc tgcgccggag aaaatgtaag atatccacca
     7681 tgtccaagga ctacaagcgc cctgaaccga aatcccgcag cagcggcagc tcgctggtca
     7741 tcggcatcct gatcggcctg gttctgggcc tcggcatcgc gctcgcggtg gcgtggtaca
     7801 tcaacaagat gcccagtccg ttcctgtccc gcccgcccgc cgtcaagggc gatcccccca
     7861 aggatg
//
LOCUS       DBNGSG010000128         5197 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2591035, whole genome shotgun sequence.
ACCESSION   DBNGSG010000128 DBNGSG010000000
VERSION     DBNGSG010000128.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5197)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5197)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5197
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2591035"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="AC158Q_03325"
     CDS             <1..467
                     /locus_tag="AC158Q_03325"
                     /inference="COORDINATES: protein motif:HMM:NF016895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR3338829.1"
                     /translation="INKMPSPFLSRPPSVKSDPPKDVQAPAPAKGEEKTAKSPAAKPR
                     FDFYKILPGSEEPATDQQLRDAQKKKSTVAARETFFLQAGAFQGATDADNLKARLALL
                     GVEATIQTTTLPDKGVWHRVRVGPYTSVEELNRTRETLRRNGVQTALIKVRE"
     gene            499..1164
                     /locus_tag="AC158Q_03330"
     CDS             499..1164
                     /locus_tag="AC158Q_03330"
                     /inference="COORDINATES: protein motif:HMM:NF013488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HIR3338830.1"
                     /translation="MNLITSFLRHAVFMLALAISSAASAQISPGKDYRLINPPQPTES
                     GKRIEVLEFFWYACPHCNALQPPLRAWLKQKPADVDFRRVPAVFDDSWLPLTYAYHAF
                     DAMGVVDRIHYDVFAAIHEQKLRLSDQAVLFDWVAKHGIDRQKFADTYNSFGVKNRGA
                     RSIEQTRNYDIPGTPAIIVDGKYLTAPSMILKPDRTVDYERYFRALDQVIAMARKERA
                     TRK"
     gene            1176..1835
                     /locus_tag="AC158Q_03335"
     CDS             1176..1835
                     /locus_tag="AC158Q_03335"
                     /inference="COORDINATES: protein motif:HMM:NF024853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HIR3338831.1"
                     /translation="MTRNRLACLARALLPLLAAACIAGAVQAQDVRARQNIEYRLISP
                     QPVETSEQIEVIDFFWYGCPYCNELQPALEDWVKRKPADVTLRRIPAILRDNWAPHAR
                     IYYTLERLGETERLHSRVYHSYHVEELHMSRPDVMEQWAAKNGIDRHRWLDAYYSPEV
                     DARVAQAFQATKRYNVQGTPSIVVDGRYLTSSSMTPSVRGVIPVIDDLIRLARQKRGE
                     Q"
     gene            1862..2641
                     /locus_tag="AC158Q_03340"
     CDS             1862..2641
                     /locus_tag="AC158Q_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019255860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3338832.1"
                     /translation="MTAQRPQHVIITGASSGVGAALARRYAGTGATLGLIARRTDRLV
                     QLAATLPVSCEICAADVRDTDAMAVAARRFMDKHGCPDTVIANAGVSVGTLTDFAEDT
                     GAFRDVIDTNVIGIVNTFQPFLSGMRGARRGTLVGIASVAGYRGLPGAGAYSASKAAV
                     IAYLESLRVELRASGIGVVTVCPGFIDTPMTEGNPYPMPFRMSADEAAAKIASLIARG
                     ESYSVIPWQMAIVARLLRVTPNWVYDRLFAAAGRKPRRPAA"
     gene            complement(2720..4561)
                     /gene="glmS"
                     /locus_tag="AC158Q_03345"
     CDS             complement(2720..4561)
                     /gene="glmS"
                     /locus_tag="AC158Q_03345"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 -
                     L-glutamine:D-fructose-6-phosphate transaminase
                     (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HIR3338833.1"
                     /translation="MCGIVGATSQRNVVDLLIDGIRKLEYRGYDSTGIAFLKGGHLER
                     LRSTGRVAQLEALVRRKRVQAGTGLSHTRWATHGAPTEANAHPHFSSVNGIEIGIVHN
                     GIVENHDPIRTRMKTLGYRFTSETDTEVMVHHVHSLVAKGLPLFEAVQRASREWEGAY
                     AVGLLSNREPGVVVGARKGSPLLVGMGQGENFIASDASALLARTRDMIYLEDGDVVEV
                     RPDGITVCDRGGRPVRRAVTASQLSADAVELGPYAHYMQKEILEQPGAVANTLEMVAN
                     ARSLQPNLFGAAAETIFKRTRNIQILACGTSYHAGLVARYWLEAIAGIPAAVEIASEY
                     RYREPVAIPGTLVVTVSQSGETADTIAALKHAQANGLTNTLTVCNAPESSLMRASKLR
                     FLTRAGPEIGVASTKAFTTQLAALFVLALILAKQRRRLAAKRERELIAQLRHLPGAME
                     AALSVEPQVRAWAVRFQAKRHALFLGRGVHFPIAMEGALKLKEVSYIHAEAYAAGELK
                     HGPLALVDSDMPVVTVAPNDRLLEKLKSNMQEVRARGGELYVFADRDARVAESEGVHV
                     IRLMDHAGPLSPILHVIPLQLLAYHVAVLRGTDVDKPRNLAKSVTVE"
     gene            complement(4655..>5197)
                     /locus_tag="AC158Q_03350"
     CDS             complement(4655..>5197)
                     /locus_tag="AC158Q_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012760903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HIR3338834.1"
                     /translation="NCVFEGEVKLADGVQIGAHCVVRDTEVGAGTRIEPFSHIDSARI
                     GENCRVGPYARLRPGARLKEEVHIGNFVEVKESDIGARSKANHLAYVGDTTVGRDVNI
                     GAGTITCNYDGANKHRTVIEDEVHIGSDVQLVAPVTVGKGATIGAGTTVWKPAPAGEL
                     TVNEKIQVTKPGWKRPVKKQ"
BASE COUNT          931 a         1670 c         1661 g          935 t
ORIGIN      
        1 acatcaacaa gatgccgagc ccgttcctgt cccgcccgcc gtccgtcaag agtgatcccc
       61 ccaaggatgt gcaggcacct gcgccagcga agggcgagga gaagacggcc aagtcgccgg
      121 cagccaagcc gcgcttcgac ttttacaaga ttctgcccgg aagcgaagag cccgccaccg
      181 accagcagct gagggatgcc cagaaaaaga agtcaacggt tgcggccagg gaaacgttct
      241 tccttcaggc cggcgcattc cagggcgcga cggatgcgga caacctcaag gcgcggctcg
      301 cgttgctcgg ggtcgaagcg acgatacaga cgacaaccct accggacaag ggggtttggc
      361 accgggtgcg cgtggggccc tataccagcg tcgaggaact caaccgcaca cgagagactc
      421 tgagacgaaa cggcgttcag accgcgctca tcaaggtgcg tgaatagtgc cggtcccagc
      481 cactttcccg gagactccat gaacttgatc acgtccttcc tgcgccacgc ggtgttcatg
      541 ctcgcgcttg ccatcagcag cgccgcttca gcgcagatct cgcccggcaa ggattaccga
      601 ctcatcaacc cgccgcagcc taccgaaagc ggcaagcgga tcgaggtact ggagtttttc
      661 tggtacgcct gcccccattg caatgctttg cagcccccgc tcagggcctg gctcaagcaa
      721 aagccggctg atgtcgactt ccggcgcgtg ccggcagtat tcgacgattc gtggctaccc
      781 ctgacgtatg cttaccatgc gttcgacgcg atgggggtcg tggacagaat tcactacgac
      841 gtgttcgcgg ccatccatga gcagaaactc cgattgtcgg atcaggcggt gctgttcgac
      901 tgggtggcca agcacggcat cgacaggcag aaattcgccg acacctacaa ttcattcgga
      961 gtgaagaacc gcggcgcgcg ctcgatcgag cagacgcgca attacgacat cccgggcacc
     1021 ccggcgatca tcgtggatgg aaaatatctg acagcgcctt cgatgatcct caagcccgac
     1081 cggaccgtcg attacgagcg ctatttccgg gcgcttgacc aggtgatcgc catggcgcgc
     1141 aaggaacgag caaccaggaa ataggggcgc cggatatgac gcgaaatcgg cttgcctgct
     1201 tggcgcgcgc cttgctgccg ctgctggccg ccgcctgcat cgccggagct gtgcaggcgc
     1261 aggacgtgcg tgcgcggcag aatatcgagt accgcctgat ctccccgcaa ccggtggaga
     1321 ccagcgaaca aatagaagtc atcgatttct tctggtacgg ctgcccgtac tgcaacgaac
     1381 tgcagccggc gctggaggac tgggtgaagc gcaagcccgc cgacgtgacg ctgcgccgga
     1441 taccagccat cctgagggat aactgggcgc cgcacgcgcg catctattac acgctggagc
     1501 ggctgggcga aaccgaacgc ctgcattcca gggtctatca cagctatcac gtcgaggaac
     1561 tccacatgag ccggcccgac gtgatggaac aatgggcagc caagaatggc atcgatcgcc
     1621 accgatggct cgacgcctat tattcgcccg aggtggacgc ccgggtggca caggccttcc
     1681 aggcaaccaa gcgctacaac gtccagggca cacccagcat tgtggtcgac ggccgctacc
     1741 tcacctcgag cagcatgact cccagcgtgc gcggcgtgat tccggtgatc gacgacctga
     1801 tccggctcgc acggcagaaa cgcggcgagc aatagggccg cagcggcggc gcgctgccat
     1861 gattacagcg cagcgcccgc aacacgttat catcaccggc gcctcgagcg gtgtgggcgc
     1921 cgcactcgcc cgtcgctatg cgggcacggg tgcaacgctg ggtctgatcg cgcggcgtac
     1981 ggaccggctc gtacagctcg ctgccaccct cccggtctcc tgtgagatct gcgccgcgga
     2041 tgtgcgcgac accgacgcga tggctgtcgc tgcacgccgg ttcatggaca aacacggctg
     2101 cccggatacc gtcatcgcca acgccggggt gagtgtcggc acgctcacgg atttcgcgga
     2161 ggatacgggc gcattccggg acgtgatcga caccaatgtg atcgggatag tgaatacgtt
     2221 ccagcccttt ctctcgggca tgcgcggcgc gcggcgcggc actctagtgg gaatcgcgag
     2281 cgttgcgggc taccgcgggc tgccgggtgc cggggcctac tccgcctcga aagcagccgt
     2341 gattgcctat ctcgaaagcc tgcgcgtgga gttgcgcgca agcggcatcg gggtcgtcac
     2401 ggtttgcccg ggtttcatcg acacgccgat gacggaggga aatccttatc cgatgccatt
     2461 ccggatgagc gccgacgaag cggcggcaaa aatcgcttcc ctgattgcgc gcggtgagtc
     2521 ctattccgtg atcccctggc agatggccat cgtcgcccgg ctgctgcgcg tgacaccgaa
     2581 ctgggtctat gaccggctct ttgccgccgc ggggcgcaag ccgcggcgtc ctgctgctta
     2641 ggctttagcg atcagctatc ggtggtcagc tacggcgggc tcgcgctcca agcgaatcgc
     2701 tggcaatcga aagtgtacgt cactcgacag tgacggattt ggcaaggttg cgcggcttgt
     2761 cgacgtccgt gccgcgcagc acggcgacgt ggtaggcgag gagttgcagc gggatcacgt
     2821 gcaggatcgg ggagagcggc ccggcatggt ccatcagacg gataacgtgc acgccttcgc
     2881 tctctgcgac gcgggcgtcg cggtcggcga agacatagag ctcgcccccg cgggcgcgca
     2941 cctcctgcat gttcgatttc agcttctcca gcagccggtc gttgggtgcc accgtgacga
     3001 ccggcatgtc ggagtccacc agcgcgagcg gcccatgctt gagctctccg gccgcatagg
     3061 cctcggcatg gatgtacgag acctccttga gcttgagcgc gccctccata gcaatcggga
     3121 agtgcacgcc acggccgagg aacagggcat gacgcttcgc ctgaaagcgc accgcccagg
     3181 cgcgcacctg cggctcgact gaaagcgcgg cctccatcgc cccaggtagg tggcgcaact
     3241 gcgctatcaa ctcgcgctcg cgtttcgcgg ccaggcgccg cctctgcttg gcaagaatga
     3301 gtgcgagcac aaagagcgcg gcgagctgcg tcgtgaacgc cttggtcgag gcgacgccga
     3361 tctccgggcc cgcgcgcgtg agaaagcgca gcttcgatgc gcgcatgagc gaggattcgg
     3421 gcgcgttgca gaccgtcagc gtgttggtca gtccgttcgc ctgcgcgtgt tttaacgccg
     3481 cgatcgtatc tgccgtttcg cccgattgcg agaccgtcac caccagggtg ccggggatcg
     3541 cgaccggttc gcggtaacgg tattcgctgg cgatctcgac ggcagcgggg atcccggcaa
     3601 ttgcctccag ccagtagcgg gcaaccaggc cagcgtgata gctggtgccg caggcgagta
     3661 tctgtatatt ccttgttctc ttgaatatcg tctccgcagc cgcgccgaac agattgggct
     3721 gcagcgaccg ggcgttggcc accatctcga gcgtatttgc gacagctccc ggctgctcga
     3781 ggatctcctt ctgcatgtag tgcgcgtagg gccccagttc cacggcatcg gcgctcagtt
     3841 ggctcgccgt gactgcgcgc cggacgggac gtcccccgcg gtcgcatacc gtgatgccgt
     3901 ccgggcgcac ttccacgaca tcgccgtctt cgagataaat catgtcgcgc gtcctggcaa
     3961 gcaaggcgct tgcatccgaa gcgatgaagt tctcgccctg gcccatcccc acgagaaggg
     4021 gcgagccctt gcgcgcgccc accaccacgc cgggttcccg gttcgacagc aggcccacgg
     4081 cataagcgcc ttcccattcg cggctggcgc gttgtaccgc ctcgaacagc ggcaggccct
     4141 tcgccaccag agaatgcacg tgatgcacca tcacttcggt gtcggtttcg gaggtgaaac
     4201 ggtagccgag ggtcttcatg cgcgtgcgga tcgggtcgtg gttttccacg atgccgttgt
     4261 gcacgatgcc gatttcgatt ccgttgactg aggagaagtg aggatgtgcg ttcgcttcgg
     4321 tcggtgcgcc gtgcgtcgcc cagcgcgtat gcgagagccc ggtgcccgcc tgcacgcgct
     4381 tcctgcgcac aagcgcttcc agctgcgcca cgcggccggt agaacgcaac ctttccaggt
     4441 gcccgccctt gaggaaagcg atcccggtcg aatcgtagcc gcgatactcc agcttgcgaa
     4501 tgccgtcgat caggagatcc acgacgttgc gctgggaggt tgcgccgacg atgccgcaca
     4561 tgagatcagt gatgaatgat gaatgatgaa tgatgaatgg gggccatcgc caaacagcgt
     4621 tgcttgccat tctgcattct gcattctgca ttcatcattg ttttttcacc ggccgtttcc
     4681 agcccggctt ggtgacctgg atcttctcgt tgacggtgag ctcacctgcc ggcgccggct
     4741 tccacaccgt ggtgcccgcc ccgatggtgg cgcccttgcc gacggtgacc ggcgcgacca
     4801 gctgcacgtc ggagccgatg tgcacctcgt cctcgatcac ggtgcggtgc ttgttggcgc
     4861 cgtcgtagtt gcaggtgatg gtgccggcgc cgatgttgac gtcgcgaccc accgtggtgt
     4921 cgccgacgta ggccaagtga ttcgccttgg agcgggcgcc gatgtcggat tccttcacct
     4981 cgacgaagtt gccgatgtgg acttcctcct tgagccgcgc gcccgggcgc agccgcgcgt
     5041 aggggccgac gcgacaattc tcgccgatgc gggccgaatc gatgtggctg aacggctcga
     5101 tgcgcgtgcc ggcgccgacc tcggtgtcgc ggaccacgca gtgggcgccg atctggacgc
     5161 cgtcggcgag cttcacctcg ccctcgaaca cgcagtt
//
LOCUS       DBNGSG010000129         5065 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3779814, whole genome shotgun sequence.
ACCESSION   DBNGSG010000129 DBNGSG010000000
VERSION     DBNGSG010000129.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5065)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5065
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3779814"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..1006)
                     /locus_tag="AC158Q_03355"
     CDS             complement(26..1006)
                     /locus_tag="AC158Q_03355"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3338835.1"
                     /translation="MKLSIFGKILAVSCVGALACASGLASGQQYPTKPVRVIVPFAPG
                     GGSDITARQFSAKLSEYLGQHFVVDNRGGAGGLIGMEATARAPADGYVIMMMSASFSA
                     TSAVQQPAFDPINAIVPVAEFGFTPFVLTVHPSLPVKTTRELITLARATKDGLNYAGT
                     GAGGATHLATELFSSMAKVKMVVIQYKSTGAAMADLLSGQVPVIVGSLLPVTPHLSTG
                     RLRALAVTTPKRWHTMPDMPTVAETLPGYDVVLWFGTMTPRGTPQPIIDRLNAGINKA
                     LQDPAIKKNLEGAGMLPSGGTPQQFGERIRKDYDRWVKVARTVGLKPTSK"
     gene            complement(1157..2215)
                     /locus_tag="AC158Q_03360"
     CDS             complement(1157..2215)
                     /locus_tag="AC158Q_03360"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR3338836.1"
                     /translation="MDQVADKLIGGIPLFAGIERAELESFLRVFQPVAVAAGATLVRQ
                     GQPADGAYLIESGDADVITALPGGGEAAVAKLGPGSVLGEMALLDGGARSATVIARSP
                     VASHFIERDGFRMLLAQRNRAAFTIQNRITRTLCQRLRELNAKIVAADASENAAPPLA
                     GASVDPASVRRGACSFDFRKFLPLLPGFRRFSAGELEGFAGRVEVLELERGQILFQQG
                     DAGELAFVVVRGALEILRAENGRRHRIGVLGPGRLCGVLALIEGESHSMSAAAREPTV
                     LLEVPRTVFDALFSGNDPVAAKFQDAINRELLQSLGRANIHLTRLISQARIRGGRREK
                     KQAEELQQALSAQDCRTA"
     gene            complement(2402..2926)
                     /locus_tag="AC158Q_03365"
     CDS             complement(2402..2926)
                     /locus_tag="AC158Q_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338837.1"
                     /translation="MLALSSTAAIAQQKKKGPARQAKPRIERIACRLGTEDQHARIAV
                     ELHNGRVNRFAYYSKWKPRTCSMEVERGDAYSQWDDTGDVTVVTLIDDTGAFLIDNGR
                     GRYHFIFREIDRMRYCGMEGKVSGSLTIFRGRSQCLLEGVMERGEDHMKDPDKPPDPE
                     ASNRQDSQGPVEKP"
     gene            3289..4638
                     /locus_tag="AC158Q_03370"
     CDS             3289..4638
                     /locus_tag="AC158Q_03370"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR3338838.1"
                     /translation="MKAWRNSNIISYSHALPDIISGKHTPREFLERCLGVIAARDKAV
                     KAFVTLNAAGARKSADAATKRYQAGKPLSPVDGMPVAVKDIIATADMPTQMNCPAFRG
                     WQSGQDAACVAALRQGGAVIIGKTVTTEFAIGYSGPTTNPFDPARTPGGSSSGSAAAV
                     GAGMVPAGLGTQTQASTLRPASYCGAVGFKPTIGALHTAGIHPLSATCDHLGILAGTL
                     EDAWRVASHISLAIGSPGNPFLTGAGPKPPAARKPRKLIRLYTRGWTEIDSDIGDAFE
                     EAVAALTSAGVEIASRDSDATVASLEQELDDGVDGALAIVAYEMKWPYEDYIARFGSV
                     IGERIHGLIERARKMSPADYEALLANRKRVQEVVRAAAAGADGFITLAASGPAILGHG
                     YTGSRTFLVYGSWLGLPAFALPLMQVNGLPLGVQLIGAPGTDGALCAVANWFMNRLN"
     gene            4773..>5065
                     /locus_tag="AC158Q_03375"
     CDS             4773..>5065
                     /locus_tag="AC158Q_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3338839.1"
                     /translation="MPTTTNPELSFRRAARADVPEIVRLLADDALGAKRERYELPLPA
                     SYLTAFEAIDSDPNNELVVACLGERVVGVLQLTFIPYLTYQGSWRALIEGVRV"
BASE COUNT          883 a         1679 c         1616 g          887 t
ORIGIN      
        1 ggggtggacc gaagggccgg ggtggttatt tggacgtcgg cttgagcccc actgtcctgg
       61 cgaccttcac ccaccggtcg taatccttgc ggatgcgctc gccgaactgc tgcggcgtgc
      121 cgccgctggg aagcatgccg gcgccttcga ggttcttctt gatggccggg tcctgcagcg
      181 ccttgttgat gccggcattg agcctgtcga tgatgggctg cggcgttccg cgcggcgtca
      241 tcgtcccgaa ccagagcacg acatcatatc cggggagcgt ctcggccacg gtgggcatgt
      301 cgggcattgt gtgccagcgc ttgggggtcg tcaccgcgag agccctcaac ctgccggtgg
      361 acagatgcgg cgtgaccggc agaaggctgc ccacgatcac cggcacctgt cccgacagca
      421 ggtcggccat cgcggcgccg gtgctcttgt actgtatgac gaccatcttg accttcgcca
      481 tgctcgagaa cagctcggtg gcgaggtgcg tggccccgcc ggcaccggtc ccggcatagt
      541 tcaagccgtc tttcgtcgcg cgcgcaagcg tgatcagctc ccgggtcgtc tttaccggaa
      601 gcgatgggtg cacggtcagg acgaatggcg tgaatccaaa ctccgccacg ggcacaatgg
      661 cgttgatcgg atcgaacgcg ggctgctgca cggcggacgt cgccgagaag ctcgccgaca
      721 tcatcatgat cacgtaaccg tcggcgggcg ccctggccgt cgcctccatg ccgatcagcc
      781 cgcccgcgcc gccgcggttg tccacgacga agtgctggcc gaggtactcg gagagcttgg
      841 ccgagaactg gcgggcggtg atatcgctgc cgccgccggg ggcgaagggc acgatcacgc
      901 ggaccggttt ggtgggatat tgctggcccg acgccaggcc ggaggcgcac gccagcgcgc
      961 cgacacacga tactgcgagg atcttgccga aaatggaaag tttcaccgcg gactcctgtc
     1021 gattggatac ggctgattga atgaacgagg gcgaattatt acacatccac cccaagaagc
     1081 gtgataagtg acgggtgata agtgatgaaa atcccctacc ccctttgcct ttttccttgt
     1141 gccttgtgcc ttgatgtcat gccgtgcggc aatcctgcgc actcagcgcc tgctgcagct
     1201 cctcggcctg tttcttctcc cgccgcccgc cgcggatgcg cgcctggctg atgaggcggg
     1261 tgaggtggat gttggcgcgc cccagcgatt gcagcagttc gcggttgatc gcgtcctgga
     1321 atttcgcggc gacagggtca tttccggaaa acagcgcgtc gaaaaccgtc ctcggaacct
     1381 cgagcagcac cgtcggctcg cgggcggccg cgctcatgct gtgggactcg ccttcgatca
     1441 gcgccagcac cccgcacagc cgccccgggc ccagcacccc gatgcgatgc cggcggccgt
     1501 tctcggcgcg cagaatttcc agtgcccccc gcaccaccac gaaggcgagc tctcccgcgt
     1561 ctccctgctg gaacaggatc tgcccccgct ccagttcgag cacttcgacg cgtccggcga
     1621 acccctcgag ctcgcccgca ctgaagcgcc tgaacccggg cagcagcggc agaaacttcc
     1681 ggaaatcgaa cgaacaggcg ccgcggcgca cgctcgcggg atccaccgat gcgccggcca
     1741 gcggcggtgc agcgttttcg gaagcgtccg ccgcgacgat tttcgcgttc aactcccgca
     1801 acctctggca cagcgtgcga gtgatgcggt tctggatcgt gaatgccgcg cgattgcgct
     1861 gggcgagcag catgcgaaag ccgtcgcgct cgataaagtg actcgcgacg ggggagcgcg
     1921 caatgacggt cgcggaacgc gcaccgccat ccagcagcgc catctcgccc agcacgctgc
     1981 ccgggccgag cttcgccacc gccgcttcgc cgccgcccgg cagcgccgtg atgacgtccg
     2041 cgtctcccga ttcgatgagg taggcgccgt cggcaggctg cccctgccgg accagggtcg
     2101 cgccggcggc aacggcgacg ggctggaaga cgcgcagaaa ggactcgagc tcggcgcgct
     2161 cgatgccagc gaacagcggt atcccgccga tcagcttgtc agcaacctgg tccaccgctc
     2221 tccctcaaaa gcgtaataga caggatacag cccatcggaa cccgccaaaa cccgtcaaag
     2281 acgttattgg acaggattaa caagattaac aggacagaaa cttcaaagac cggttatttc
     2341 tgtcttatgg attgaatcct gtaaatcttg taaatcctgt ctattgatgc gtttgacggg
     2401 tttacggttt ttcgacgggg ccttgtgaat cctgtcgatt gcttgcctcc gggtcgggag
     2461 gcttgtccgg gtccttcatg tggtcctcgc cgcgctccat cactccctcc agcagacatt
     2521 gcgagcggcc gcggaagatg gtgaggctgc cgctcacctt gccttccatg ccgcagtagc
     2581 gcatgcggtc gatttccctg aagatgaaat ggtagcggcc ccgtccattg tctatcagaa
     2641 acgcgcccgt atcgtcgatc aaggtgacaa cggtcacgtc gccggtgtcg tcccactggc
     2701 tgtaggcgtc gccgcgctcg acctccatcg agcaggtgcg gggcttccat ttgctgtaat
     2761 aggcaaagcg gttgaccctg ccgttgtgca attcgaccgc gatgcgcgca tgctgatcct
     2821 cggtgcccag ccggcacgcg atcctctcga tcctgggttt cgcctgccgg gcggggcctt
     2881 tcttcttctg ttgcgcgatg gcggcggtgg acgacagggc cagcatgacc gcgcagcaaa
     2941 cggcaaacca gcgccctatt cccaaaactt ctcctctgtc cgcagaacgg gaccgtttcg
     3001 ccccgacgcg ttattattta ccgcgcgttg aacggcgggt tgccgtccgc tcgaacgcgt
     3061 ggagttaaac tggcccctgg cgtcctgctt taccgcgcgt tgaccggcgg gttaccgtcc
     3121 gctcgaacgt gcggagttaa actggcgccc ggcgtcctgt tttcccgcgc gttgaacggc
     3181 gggttaccgt ccgctcgaac gcgcggagtt aaacgggcgc cccggcgtcc tgtttaacac
     3241 gaattataag gccgcggctc attgcgcgcg gcccccggag tccggatcat gaaagcctgg
     3301 cgcaattcca acatcatctc ttactcccac gcactgcccg atatcatttc aggcaagcac
     3361 acgccgcggg agttcctcga gcgctgcctc ggagtcatcg ccgcgcgcga caaagcggtg
     3421 aaagccttcg tcacgctgaa cgccgcgggc gcccgcaaga gcgcggacgc cgccacgaag
     3481 cgctaccagg cgggaaagcc gctctcaccg gtggatggca tgccggtcgc ggtgaaggac
     3541 atcatcgcca ccgccgacat gccgacccag atgaactgcc ccgcgttcag gggctggcaa
     3601 tccggccagg acgccgcctg cgtcgcggcc ttgcggcaag gcggcgcggt catcatcggc
     3661 aagaccgtca ccaccgagtt cgcaatcggc tattccgggc cgaccaccaa cccgttcgac
     3721 ccggcgcgca cgcccggcgg ctcctcgagc ggctcggctg ccgcggtcgg cgccggcatg
     3781 gtgccggcgg ggcttggcac gcagacgcag gcttccacgc tgcggcctgc gtcctattgc
     3841 ggcgcggtgg gcttcaagcc gacgatcggc gcgctgcaca ccgcgggcat tcatcccctt
     3901 tcggccacct gcgatcatct cggcatcctc gccggcacgc tggaggatgc gtggcgtgtc
     3961 gcctcgcata tttctctcgc gatcggcagc cccggaaatc ccttcctcac cggggcgggc
     4021 ccaaaaccgc cggcggcgcg caagccgcgc aagctgatcc ggctctacac ccgcggctgg
     4081 acggagatcg acagcgatat cggggatgcg ttcgaggaag ccgtggccgc cctcacgtca
     4141 gccggcgtgg aaatcgccag ccgtgacagc gatgccacgg tcgccagtct cgagcaggag
     4201 ctcgacgacg gcgtggacgg cgcgctcgcc atcgtcgcct acgagatgaa gtggccgtac
     4261 gaggattaca tcgcgcgctt cggcagcgtg atcggcgagc gcatccacgg gctcatcgag
     4321 cgggcgcgca agatgtcgcc ggcggattac gaggcgctgc tcgccaatcg caagcgggtg
     4381 caggaggtgg tacgcgcggc cgctgcgggt gcggacggat tcatcaccct cgccgcttcc
     4441 ggcccggcga tcctcgggca cggatacacc ggcagccgca ccttcctcgt gtatggctcg
     4501 tggctggggc tgcctgcttt cgcgctgccg ctcatgcagg tgaacgggct gccgctcggc
     4561 gtgcagttga tcggcgcgcc ggggacggac ggggcgctgt gcgcggtggc gaattggttt
     4621 atgaaccgtt taaattagcc gccaagacgc caagagaacc tttacaaaat actgaaccgc
     4681 caagcccgcc aagaaatcaa gaacagaata aaaatccaaa caattggcca cagagagcac
     4741 agaggacaca gagaagaaag tccgccgcag caatgccaac caccacgaat cctgaattgt
     4801 cgttccggcg ggcggcgcgg gcagacgtgc cggagatcgt gcggctactg gcggacgatg
     4861 ccctgggcgc gaagcgcgag cgctacgagc tgccgctgcc cgcgagctat ctcaccgcat
     4921 tcgaagcgat cgactccgac cccaacaacg aactcgtcgt cgcctgcctg ggcgagcggg
     4981 tcgtcggcgt gctgcagctc accttcatac cgtacctgac gtaccagggc agctggcgcg
     5041 cgttgatcga aggggtgcgc gtgga
//
LOCUS       DBNGSG010000130         6513 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3950024, whole genome shotgun sequence.
ACCESSION   DBNGSG010000130 DBNGSG010000000
VERSION     DBNGSG010000130.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6513)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6513)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6513
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3950024"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="AC158Q_03380"
     CDS             complement(<1..325)
                     /locus_tag="AC158Q_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR3338840.1"
                     /translation="MPRTARQQPLSEARAALSEQYAAVRARSLEICRPLAIEDYVIQS
                     MPDTSPPKWHLAHVTWFFENFLLAPFLRGYQAYHPAYAFLFNSYYETVGRFFPRLQRG
                     LLSRPT"
     gene            complement(517..3771)
                     /locus_tag="AC158Q_03385"
     CDS             complement(517..3771)
                     /locus_tag="AC158Q_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HIR3338841.1"
                     /translation="MAIHPKTIYTKTPKGVLEVKNKTAKLDRSQGKVFVAVDGKSTVT
                     DLVKKADLDEKELNEVLEKLTTDGFIRVFSAPETAPAPAAAKPAGAAAASAADDFDFT
                     TPEAMTKLNAEAEARAKAEAQAKARALTAARAAAEAKVKQEAEARARAQAEARVKAEM
                     DAKVKAEAAAKAAAEARVKAEAEAKAAANNAKLKAEAEARVKAAMEAKIRADTEAKAR
                     AEAEARARIEAEAKIEAETKARIEAESRARAEAEAQAHADAETKARTAVDAQMKALQE
                     ALAQAEARAKREAEQRARMEAEIKAHQEAERKAREEAEARAKAAEEAVAQALAKAEEA
                     AKAKAEAESRAATQAIAGGGGDADAQARMAAAMQALEEAKAKARAEAEARVEAERKAQ
                     IEAEARQREEADRKVREEQERKAREEADARAKAQMKELQDQANRAKHEADARSEIERK
                     AREEAEARMAAERQARVEAEAKAELERKSRDEALAKARVEAEAKARAEVEGMIAAERK
                     AREAAEARAKQEIASRIDSEKKARADAERQAELARKAHEAAETRARELANSGNAQAAQ
                     KARAEAEAIARKADEAVAQANARFEAERAARAQAEERAKAEAVARVMQEQQLRANSEG
                     DIQARVQAEIKQRERAEMEAEARYRQEAAARAKASAEARRQREEESKAAGAAAPARVR
                     KATNWKLTLGIGAVVLLAAIIGLLEVVPLNNFIGGAQQIMSKRLGVPVTISRLSYSLL
                     RQELILERVGIGKLQETKVDAIKVSAFPTSLLGDVKSFGEVEAVSVNADQDALALLPE
                     WVKPQAGEQPLHVRRLKLKSVKLSVKALELSPFDADITLGADGALQRALLTDGKLRLE
                     LSPKDKGIRVALEATSWKPPLGPALEFDDIALEALFEPQKATITSIEGKIGFAPVKGS
                     ARASWSSGGISVEGDFSVTKGDLTRLMGTFTRDFTATGVLAANGTFALQGTTLANLFD
                     QPRVEATFSLEQGVLNNVDLVRGIQSAARDGVRGGNTRYNTLTGSLRVAAKTYSYRQL
                     QLASGPMNASGNLDVAASGDLSGRVSAELGSKTFTVARGTLIVGGNLKTPSLRQ"
     gene            4031..4996
                     /locus_tag="AC158Q_03390"
     CDS             4031..4996
                     /locus_tag="AC158Q_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338842.1"
                     /translation="MTLHERRNDYDVTNRVNTTDPQDVSQEVCRIYQELYQQATPKNL
                     GKAFADLGRLYRGENPGYHECDTDYHDVQHVLDVTLGMARLMNGCVRTTSTDIITERL
                     FRFGIILALYHDCGYIRHRKDTKHANGAEYTAVHVSRGGRFLEEYLPDIGMADLAQAA
                     SRTIHFTGYEMPTDKIKVPGPEFRLIGNLLGSADILAQMADRCYLEKCYDRLYPEFVR
                     GGIARKRNKDGSEEIIFASAADLIFKTPRFYQGATKRLKQDLGGCYNYIEEHFGGQNL
                     YFDELEKNINHAKAIAVEGDISLLRRRPPKTTEGAELEQPQPHLI"
     gene            complement(5063..6031)
                     /locus_tag="AC158Q_03395"
     CDS             complement(5063..6031)
                     /locus_tag="AC158Q_03395"
                     /EC_number="1.6.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - quinone reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HIR3338843.1"
                     /translation="MKAVEISSPGGPEVLKPAERPVPEPGAGEVLIKVTAAGVNGPDL
                     MQRKGLYPAPPGATDLPGLEVSGEIVAAGAKAGRWSAGDRVAALTNGGGYAEYCVADA
                     GHCLPVPKGITLADAGGLPETFFTVWSNVFLGAGLAAGETFLVHGGAGGIGTTAIQLA
                     KAFGARVIATDSPAERCAICRDLGADRVIDYKQEDFVEIVREAGGANVILDIVGGPNI
                     EKNFKAATHDCRIIQLAFAAGSRVEINLMPVMLKRLTYTGSTLRTRPPAYKAKVAQEL
                     EAKVWPEIERGRIRVVTRQTFPLAEASRAHALMESAQHTGKILLTV"
     gene            6170..>6513
                     /locus_tag="AC158Q_03400"
     CDS             6170..>6513
                     /locus_tag="AC158Q_03400"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3338844.1"
                     /translation="MAAILRTVMAALVTAAGAGSDPVHAQSFPSRPMRIIVPVPAGGG
                     VDGVLRLLDSRMTELTGQNIIIDNRPGASGNVGTSLAARAAPDGHTVLATTLSLVLNP
                     NLFNSPGHDVLR"
BASE COUNT          907 a         2256 c         2085 g         1265 t
ORIGIN      
        1 cggtaggccg ggacagcaga ccgcgctgca ggcgcgggaa aaacctcccc accgtctcgt
       61 aataggaatt gaacaggaag gcgtaggccg ggtggtaggc ctggtagccc ctcaggaagg
      121 gcgcaagcag gaaattctcg aagaaccagg tgacgtgcgc caggtgccat ttcggcgggc
      181 tcgtgtccgg catgctctgg atcacgtaat cctcgatggc cagcgggcgg cagatttcga
      241 gggatcgtgc ccgcaccgcg gcgtactgct ccgataatgc cgcgcgagct tcggaaagcg
      301 gctgctgcct ggcggtgcgc ggcatgttgt ctcctcggtt tgacggcggt aattatcttg
      361 accgtcaatt attctgatac gttccgcgcc gcgcggcgga tgcggggcct ggcgccgcgt
      421 ggacagcgcc tcaaaaaaac aacaaagcct gcgtcattca ggccttgttg ccggtcgatt
      481 cacgattcac gactcacgaa tcacgcctca tccaggctac tgcctgagcg acggcgtctt
      541 gaggttgccg ccgacgatga gcgtgccgcg cgcgacggtg aacgtcttcg atcccagctc
      601 ggcgctgacg cgtccggaca ggtcgccgct ggccgcgacg tccaggttgc cgctggcgtt
      661 catcgggccg gaggccagct ggagctggcg gtaggagtag gtcttggccg caacccgcag
      721 cgagccggtg agggtgttgt aacgggtatt gccgccgcgc accccatcgc gcgccgcaga
      781 ctggatgccg cgcacgagat cgacgttgtt cagcacgcct tgctcgaggc tgaacgtcgc
      841 ctccacccgg ggctggtcga acaggtttgc caacgtggtg ccctggagcg cgaaagtgcc
      901 gttggccgcc agcactccgg tcgcggtgaa atcgcgcgtg aacgtcccca tcagcctcgt
      961 gagatcgccc ttggtcacgc tgaaatcgcc ttcgacgctg atgccgccgc tgctccagct
     1021 cgcccgggcg ctgcccttca ccggcgcgaa cccgatcttg ccctcgatgc tggtgatggt
     1081 cgccttctgc ggctcgaaca gcgcttccag cgcgatgtcg tcgaattcca gcgcgggccc
     1141 gaggggcggc ttccagctgg tcgcttccag cgcgacgcgg atgcccttgt ccttgggaga
     1201 gagttcgagc ctgagcttcc cgtccgtcag cagcgcccgc tgcagcgcgc cgtcggcgcc
     1261 cagcgtgatg tccgcgtcga agggggagag ctccagcgcc ttgacgctta gcttgaccga
     1321 tttcagcttc aggcgccgca cgtgcagcgg ctgctcgccg gcctgcggct tcacccattc
     1381 cggcagcagc gcgagggcgt cctggtcggc gttgaccgag acggcctcga cttcgccgaa
     1441 gctcttgacg tcgccgagca gcgatgtcgg gaacgcgctg accttgatgg catcgacctt
     1501 ggtttcctgc agcttgccga tgccgacgcg ctcgagtatc agttcctggc gcagcaggga
     1561 atagctcagc cgggaaatcg tgaccggcac gcccagccgc ttcgacatga tctgctgcgc
     1621 gccgccgatg aaattgttga gcggcacgac ttccagcagc ccgatgattg ccgcgagcag
     1681 caccacggcg ccgatgccga gggtcaactt ccagttggtc gctttcctga cgcgggccgg
     1741 ggcggcggcg cccgccgcct tcgattcctc ctcgcgctgg cggcgcgcct cggccgatgc
     1801 cttggcccgc gccgcggctt cctgccggta gcgcgcctcg gcctccatct cggcgcgctc
     1861 gcgctgcttg atctcggcct gcactcgcgc ctggatatcg ccctctgaat tggcgcgcag
     1921 ttgctgctcc tgcatgacgc gcgctaccgc ctcggccttg gcgcgctcct cggcctgcgc
     1981 gcgtgccgca cgctcggctt cgaatcgcgc attggcctgc gcgaccgctt catcggcctt
     2041 gcgcgcgatg gcctcggctt ccgcgcgcgc tttctgcgcc gcctgcgcgt tgccgctgtt
     2101 cgcgagttcg cgggccctcg tctcggccgc ctcgtgcgcc ttgcgcgcga gctcggcctg
     2161 gcgctcggcg tccgcgcgcg ccttcttttc cgagtcgatc ctggaggcga tttcctgctt
     2221 ggccctggcc tcggctgctt cgcgcgcctt gcgctcggca gcgatcatgc cctcgacctc
     2281 tgcgcgggcc ttggcttcgg cttccacgcg tgccttggcg agcgcctcgt cgcgcgattt
     2341 gcgctcgagt tccgccttgg cttcggcttc cacgcgcgcc tggcgctcgg cggccatcct
     2401 ggcctcggct tcctcgcgcg ccttgcgctc gatctcggac ctggcgtcgg cttcgtgctt
     2461 ggcgcggttg gcctggtcct gcaattcctt catctgcgcc ttggcgcgcg catccgcctc
     2521 ctcgcgcgcc ttgcgctcct gctcctcgcg caccttgcgg tccgcttcct cgcgctggcg
     2581 cgcctcggct tcgatctgcg ccttgcgttc ggcctcgacc cgtgcctcgg cctctgcccg
     2641 tgccttggcc ttggcttcct cgagcgcctg cattgccgca gccatgcgcg cctgggcgtc
     2701 cgcgtcaccg cccccgcccg cgatcgcctg cgtggctgcc ctggattcag cttcggcctt
     2761 ggctttcgcg gcttcctccg ccttggcgag cgcctgagcc accgcctcct cggccgcctt
     2821 tgcgcgcgcc tcggcttctt cgcgcgcctt gcgctcggcc tcctggtgcg ccttgatctc
     2881 ggcctccatc cgcgcgcgct gctcggcttc ccgcttggcg cgcgcctcgg cctgggccag
     2941 cgcttcctgc agcgctttca tctgggcgtc cacggccgtg cgcgccttgg tctcggcatc
     3001 cgcgtgggcc tgcgcctcgg cctcggccct ggcgcgcgat tccgcttcga tcctggcttt
     3061 ggtctccgct tcgatcttgg cttcggcttc gatcctggcg cgcgcttcgg cttccgcccg
     3121 cgctttcgct tcggtatccg cacggatctt ggcttccatc gccgccttga cccgcgcttc
     3181 ggcttccgcc ttgagcttgg cgttattggc tgctgctttt gcttccgctt cggccttgac
     3241 tcgcgcttcg gccgcggctt ttgccgccgc ctcggccttg actttggcat ccatctcggc
     3301 cttgactctg gcctcggctt gcgcccgagc ccgggcttcc gcttcctgct tgaccttggc
     3361 ttcggccgcc gcgcgcgcag cggtaagcgc acgggccttc gcctgggctt cggccttggc
     3421 ccgcgcttcg gcctcggcat tgagcttggt catcgcttcg ggcgtggtga aatcgaagtc
     3481 atctgccgcg ctggcggcgg ccgctccagc cggcttcgcg gctgccgggg ccggcgcggt
     3541 ctcgggagcg gagaacaccc ggatgaagcc gtcggtggtc agcttctcga gaacctcatt
     3601 cagttctttt tcgtccaggt ccgctttctt gaccaggtcc gtgacggtgg acttgccgtc
     3661 caccgcgaca aataccttgc cctgggaacg gtcgagtttg gcggtcttgt tcttgacttc
     3721 aagcacgccc ttgggcgtct tggtgtagat ggtcttggga tgtatggcca tgagcggatt
     3781 ttttcgggta tttcaatgcg atgcaaggcc ggctgccgca accgcggttg cggcggggcc
     3841 gttcgttttc atcctccccg ggcggcgtta cgaggctgtc accgtctgaa aaggcattaa
     3901 cttccatgcc ttatccggca ttgcaagaac aaggtcatta tagtctaata ccgcgctaac
     3961 gcgctcctgg aacaaaagca aaagcggcgc ggtcgaattg gtgtcgattg gcctaccggg
     4021 tgagaagacg atgaccctgc acgagcgccg caacgactac gacgtcacca accgcgtcaa
     4081 caccaccgac ccgcaggacg tatcccagga agtctgccgc atctaccagg agctctacca
     4141 gcaggccacg ccgaagaatc tcggcaaggc tttcgccgac ctggggcgcc tctaccgggg
     4201 ggaaaatccc ggctaccacg agtgcgacac cgattaccac gacgtccagc acgtgctcga
     4261 cgtcacgctg ggcatggcgc gcctgatgaa cgggtgcgtg cgcacgacca gcaccgatat
     4321 catcaccgag cggctgttcc gcttcgggat catcctcgcg ctctaccacg actgcggcta
     4381 catccgccat cgcaaggaca ccaagcacgc gaacggcgcg gaatacaccg ccgtgcacgt
     4441 gtcgcggggc gggcgcttcc tcgaggagta cctcccggat atcggcatgg cggacctggc
     4501 gcaggccgcc tcgcgcacga tccacttcac cggctatgag atgcccacgg acaagatcaa
     4561 ggtgccgggt cccgagttca ggctgatcgg caacctgctg ggcagcgccg atatcctcgc
     4621 gcagatggcg gaccgctgct acctcgagaa atgctacgac cggttatacc cggaattcgt
     4681 gcgcggcggc atcgcccgca agcgcaacaa ggacggcagc gaggaaatca ttttcgcctc
     4741 ggctgcggat ctcatcttca agaccccgcg tttctaccag ggcgcgacca agcgcctgaa
     4801 gcaggacctc ggcggatgct acaactacat cgaggagcat ttcggcggcc agaacctgta
     4861 tttcgacgaa ctcgagaaga acatcaacca cgccaaggcg atcgcggtcg agggcgacat
     4921 ctcgctgctg cgccgccgcc cgcccaagac caccgagggc gccgagctcg agcagccgca
     4981 gccgcatctg atctagaaaa agtgactcgt tgctcgttcc tcgttgcggg tgcggaacag
     5041 aaaacgagaa acgagaaacg aatcacaccg taagcaggat cttcccggtg tgctgcgccg
     5101 attccatcag cgcgtgcgcc ctggaggctt ccgccagcgg aaaggtctgg cgcgtgacca
     5161 cccgtatgcg ccccctttcg atttccggcc agactttcgc ctcgagttcc tgcgcgactt
     5221 tcgccttgta ggccgggggc cgggtgcgga gggtggaccc ggtataggtc aaccgcttca
     5281 gcatgacggg catcaggttg atctccaccc tggagccggc cgcgaacgcg agctggatga
     5341 tgcggcagtc gtgggtggcg gccttgaaat tcttctcgat attcgggccg ccgacgatgt
     5401 cgaggatgac gttcgcgccg ccggcctcgc gcacgatttc gacgaaatct tcctgcttgt
     5461 agtcgataac gcggtcggcg cccaggtcgc ggcagatcgc gcagcgctcc gcggggctgt
     5521 cggtcgcgat gactcttgcg ccgaacgcct tggcgagctg gatggccgtc gtgccgatgc
     5581 cgcccgcgcc accgtggacc aggaacgttt ctcccgccgc cagcccggca cccaggaaga
     5641 cgttgctcca cacggtgaaa aaggtctccg gcaggccgcc cgcgtcggcg agcgtgatgc
     5701 ccttcgggac cggcaggcag tgcccggcgt ccgcaacgca atattcggcg tatccgccgc
     5761 cgttggtgag cgcggccacc cggtccccgg ctgaccagcg cccggccttg gcgcctgcgg
     5821 cgacgatttc gccggacact tcgaggccag gcaggtccgt agcgccgggc ggggcagggt
     5881 agagcccctt gcgctgcatc aggtccgggc cgttcacgcc ggcggccgtc accttgatca
     5941 acacctcgcc cgcgcccggc tcgggcacgg gccgttcggc cggtttcagc acttcgggac
     6001 cgccgggact ggaaatttcg acggccttca ttcgtttggg caggttggca ttcattgcgg
     6061 tgggccggtg atttcgagcg caggattcag cccctatact agcattcgtg aaatcctcgt
     6121 ttatggccgc gcggcggcga tacgcttgat gagtggtgcg ggcgcgcccg tggcggcaat
     6181 cctgcgaaca gtcatggcgg ccctggtgac cgcggccggc gcgggcagcg atccggtgca
     6241 cgcgcaatcc tttccgagcc ggccgatgcg catcatcgtg ccggtcccgg ccggtggcgg
     6301 cgtggacggc gtcttgcgcc tgctcgacag ccggatgacg gagctgaccg ggcagaacat
     6361 catcatagac aatcgtcccg gcgcgtccgg caatgtcggc acgagtctcg cggcgcgcgc
     6421 cgcgcccgac gggcataccg tgctggcgac gaccctgtcg ctggtgttga accccaacct
     6481 gttcaacagc ccggggcacg acgtgctgcg tga
//
LOCUS       DBNGSG010000131         7950 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1743817, whole genome shotgun sequence.
ACCESSION   DBNGSG010000131 DBNGSG010000000
VERSION     DBNGSG010000131.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7950)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7950)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7950
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1743817"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..1040)
                     /locus_tag="AC158Q_03405"
     CDS             complement(189..1040)
                     /locus_tag="AC158Q_03405"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA dioxygenase family protein"
                     /protein_id="HIR3338845.1"
                     /translation="MDNAERRHYRHLSVHPIAGALGAEIRGVDIARLLEEAVVAEIRQ
                     AFLDHLVIFFRDQKLAPQQLLSFSRRFGEPMEYPQLKGLPECPLITPVVKLEHERTNF
                     GGVWHSDTSYLERPPMASMLYALETPPVGGDTLFANQYLAYETLSDGLKQTLTGLVGI
                     NSSTKADVTRSREDRLRAAGAETKALTGEHPVVRAHPETGRKALYVNFGHTTQFRGWS
                     ETESAPLLGYLFQHQVKPEFTCRFHWEPGSLAFWDNRCAQHNPVNDYHGFRRVMHRVT
                     LAGDTPR"
     gene            complement(1083..1928)
                     /locus_tag="AC158Q_03410"
     CDS             complement(1083..1928)
                     /locus_tag="AC158Q_03410"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTD2 family dehydratase"
                     /protein_id="HIR3338846.1"
                     /translation="MTLDIAPLKKWIGRQETAGDSLPPFPAAALAASLDRGDAPGMGG
                     PLPPLWHWVYFLELHRQAELAGNGHARLGGFLPPVPLPRRMFAGARLSFLRPLRIGEE
                     ARRVSTIADVKLRKGASGDLVFVVVRNEIAGSGGPAIREEQDLVYREAARPEPAPPAP
                     RRSPAKAAWSREMKFDVALLFRYSALLFNAYRIHYDRPYAAEQGYSGLVVHGQLIATL
                     LADLVRRNTDAPVSSFRYRSVSPLTDGSPCLLCGAPDGDLVNLWAEDANGRLVVQAEA
                     ELSAR"
     gene            complement(1933..3120)
                     /locus_tag="AC158Q_03415"
     CDS             complement(1933..3120)
                     /locus_tag="AC158Q_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR3338847.1"
                     /translation="MLPLAGVRVLAVEQYGAGPFGTMFLADQGAEVIKIENPNDGGDM
                     SRAVGPHFFASGDSQFFHSFNRNKKSLTLDLARPEGQAVLHDLVRGADAVASNLRGDV
                     PAKLGLVYDRLREHNPAIVCAHLSAYGRSGPRADWPGFDYLMQAEAGYFSLTGEPGAP
                     PARFGLSIVDLMTGLGLAYALLAALTAARATGVGRDIDVSLFDMALHNTSYLATWYLN
                     GGVVTGRLPRSAHPSLTPCQLYRTRDGWIYLMCNKEKFWPALCGKLGRPEWGGDLRFR
                     RFADRLKHRDLLTGMLDAELERRTTAEWLALFAGVVPAAPVNDLKQALENPFVTEHGR
                     LQAIPRPGQEAYRMIAPPVRCADENAPARPAPALGEHTDALLKEIGYGADRIRELRES
                     KVI"
     gene            complement(3252..4403)
                     /locus_tag="AC158Q_03420"
     CDS             complement(3252..4403)
                     /locus_tag="AC158Q_03420"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3338848.1"
                     /translation="MTTSDGQDALILQSVDRFLEREVRPVVRALEAADTYPTAIVEKL
                     RTLGMFGATIAPEHGGLGLTAVTYAKIVERMSAVWMSVPGLFNSHLIMAGAVQRHGTA
                     EQKARYLPRFASGELRGGIALTEPDCGTDLQAVRTQARREGDHYVVNGAKTWITNSAE
                     GHLLAVLVKTDSKAQPPHKGMSLLLIEKGPGFKVVRKLEKLGYRGIDTAELLFEDCRV
                     PAVNLIGTQEGRGLQQVLNGLELGRINVAARGAGLARACLEESLRYGQLRKSFGKPIA
                     EHQAIQLKLADMATRVEASRLLVEQAARAYDAGRRCDLEAGMAKLYATEAALENSLEA
                     MRIHGAYGYSKEFNLERYYRDAPLLAIGEGTNELQRIIIARELVKRNPA"
     gene            complement(4527..5045)
                     /locus_tag="AC158Q_03425"
     CDS             complement(4527..5045)
                     /locus_tag="AC158Q_03425"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR3338849.1"
                     /translation="MTVERKRIGPNRYRESFGRYFEDFKVGDVYEHRPGRTISEADNT
                     WFTLLTMNTHPLHFDAAYAAKSEFGRPLVNSCLTLAMVTGMSVSDLSQKAVANLGWDK
                     VTLTAPVFAGDTIYAESEVLAVRESKSRPTQGIVTVRTTGRKADGTAIMNFERSILVP
                     RRGHAADDLAGY"
     gene            complement(5190..6542)
                     /locus_tag="AC158Q_03430"
     CDS             complement(5190..6542)
                     /locus_tag="AC158Q_03430"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3338850.1"
                     /translation="MPVEVRERAKALVLDLAGISVRARNEAESTPAMVAAAGHLGLAN
                     GACTVIGDVAGYTPAGAAMLNGTLAHSLDFDDTHASGSLHPSAPIVPAAFAAAEMTGA
                     EGKALIAAIVAGYEVQIRLSLALDPKAHYDRGFHPTATCGAFGAAAAAGRLLGLDPEG
                     YANAFGIVLSMSAGSMQFLVNGAWTKRSHVGHAAMCGLVAATLAREGYKGASEAFEGK
                     WGFFHAYAPSADAAKAVDGFGRRWETLKIAVKPYPSCRYTHAPLDGILALAREHGINA
                     GEVEEVAVGLPEPGWKITGDPEPAKQSPKSVVDGQFSMAFCAAVALRSGGLEWDDYAR
                     HLGDPATLALCKRVRTRVDPKAQADFPAEMSGSVRIRTARGAFESYVRVPKGEPANFL
                     TAAELRAKFDGLAGPYLSARRLDELAGALLALEQAKDIGAVLRLSRPEQPQRISAAGR
                     "
     gene            complement(6692..7216)
                     /locus_tag="AC158Q_03435"
     CDS             complement(6692..7216)
                     /locus_tag="AC158Q_03435"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HIR3338851.1"
                     /translation="MKPLIIAATAAVAVLLGACQTTPTEAPRANAGLKPTKGNKTIGE
                     ATFEQVGSKVHVVIFVQGLRPGQEHGLHIHEKGDCSSGDGMSAGGHFNPLGKPHGMPG
                     SAQSHAGDLPALKANKAGRANVKVEVDNITVTPGPTSIIGRAVIVHADPDDFKTQPTG
                     NAGARLACGVIQAG"
     gene            7379..>7950
                     /locus_tag="AC158Q_03440"
     CDS             7379..>7950
                     /locus_tag="AC158Q_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013390873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338852.1"
                     /translation="MRQAAAIPAPALAGCPQAPEGADLIEWYYERGYSDGFPLVPPTP
                     EKIAAVVDNLGGDAGRLVSRVPPRWGSLTREVLAINMVMAGCRPEYAPVVEAALLALC
                     DTRFNLNGVQATTHMASPLLVVNGPARHAIGMNAGCNVFGSGNRANATIGRALRLVLL
                     NVGGGWPGLLDKSTLGHPGKYTYCIAENEEA"
BASE COUNT         1338 a         2639 c         2568 g         1405 t
ORIGIN      
        1 atcgggtttg atgatggata acgtgcgttc aaccgccatg aaacttcccc tgcccttcga
       61 gatttgaatt tagctcgcga ggataccacg ataaggcttt gaaatgaagc ggtttcatga
      121 caattcaccc ggcaaaacaa acgcttgcca ggttgccgcc gtcagagggc gggaacgcgc
      181 agcggccgtc agcgcggcgt gtcgcctgcc agtgtgacgc ggtgcatgac gcgccggaat
      241 ccgtgatagt cgttcaccgg gttgtgctgg gcgcagcggt tgtcccagaa ggcgagcgaa
      301 cccggctccc agtgaaaacg gcaggtgaac tccggcttga cctggtgctg gaagaggtag
      361 ccaagcagcg gcgcgctttc cgtctccgac cagccccgga actgcgtggt gtggccgaaa
      421 ttcacgtaaa gcgccttgcg cccggtctcg ggatgggcgc gaaccaccgg gtgctccccg
      481 gtcagcgctt ttgtctcggc tcccgccgcg cgcagccgat cctcccgcga ccgggtgacg
      541 tcggccttgg tcgaactgtt gatgccgacc aggcccgtca gcgtttgctt cagcccgtcc
      601 gagagcgttt cgtaggccag gtactggttg gcgaacagcg tgtcgccgcc gaccggcgga
      661 gtttccaggg cgtagagcat gctcgccatc ggcgggcgct ccagatacga cgtgtccgag
      721 tgccacacgc cgccgaagtt cgtgcgctcg tgttccagct tgactaccgg cgtgatcaat
      781 gggcactccg gcaggccctt cagctgcggg tactccatgg gctcgccgaa tcgccgcgag
      841 aaggagagca actgctgcgg cgccagtttc tggtcccgga aaaaaattac caggtgatcg
      901 agaaaagcct ggcggatctc cgcaacaacg gcttcctcga ggagccgtgc aatatccaca
      961 ccgcggatct cggcgccgag ggcgccggcg atcgggtgca ccgagaggtg gcgataatgg
     1021 cgacgttccg cgttatccat tgttggcctg ccccgcaaga tcagtattca tggtacacct
     1081 tttcagcgtg ccgacagttc ggcctcggcc tgcacgacca gcctgccgtt cgcatcttcg
     1141 gcccacaggt tcaccaggtc gccatccggt gctccgcaga gcaagcacgg cgagccgtct
     1201 gtcagcgggc tcaccgaccg gtagcggaat gaactgacgg gcgcatccgt gttccggcgc
     1261 accaggtccg cgagcagcgt ggcgatcagc tgcccgtgga ccacgaggcc agaatagcct
     1321 tgttccgctg catacggccg gtcgtagtgt atgcggtagg cgttgaagag cagcgccgaa
     1381 taccggaaca gcaacgccac gtcgaatttc atctcccggc tccaggccgc tttcgcggga
     1441 gaacggcgcg gcgcgggtgg agccggttcc gggcgtgccg cttcgcgata aacgaggtcc
     1501 tgctcttccc tgatggccgg cccgccggat ccggcaattt cgtttcgaac gaccacgaac
     1561 accaggtcgc cgcttgcgcc tttcctcaac ttcacgtccg caatggtcga gacgcggcgc
     1621 gcttcctcgc cgatgcgcaa cggccgcaga aacgacaggc gcgccccggc gaacatgcgc
     1681 cgcggcaacg gcacgggggg caggaagccg ccgagccgcg cgtggccgtt gccggccagc
     1741 tccgcctgcc ggtgcagttc caggaaatag acccagtgcc acagcggagg cagcggcccg
     1801 cccatccccg gcgcgtcgcc gcggtcgagc gatgctgcca gcgcggcagc cggaaacggc
     1861 gggagggaat cgcccgcggt ctcctgccgg ccgatccatt tcttcagcgg agcaatgtcc
     1921 aatgtcattc ggctagatga ccttggattc ccgcagctcg cgaatgcggt cagcgccgta
     1981 accgatctcc ttcagcagcg catccgtatg ctcgccgagc gcgggggccg ggcgggccgg
     2041 cgcgttctca tcggcacacc ggaccggcgg ggcgatcatg cgataggcct cctgtcccgg
     2101 gcgcgggatc gcctgcaacc ggccgtgctc ggtcacaaac gggttttcga gggcctgctt
     2161 gagatcgttg accggtgcgg ccgggacgac cccggcgaac agcgccagcc actcggcggt
     2221 cgtgcgtcgc tcgagttcgg catcgagcat accggtgagg agatcgcgat gcttcaggcg
     2281 gtccgcgaaa cggcgaaagc gtaaatcccc gccccactcg ggacgcccga gttttccgca
     2341 cagcgccggc cagaacttct ccttgttgca catcaggtag atccagccgt cgcgcgtgcg
     2401 gtagagctgg cacggcgtca gcgaaggatg cgccgagcgc ggcaggcggc cggtgacgac
     2461 gcccccgttc agataccacg tggcgaggta ggacgtgttg tgcagcgcca tgtcgaacag
     2521 actgacgtca atgtcgcgcc ccacgccggt cgcgcgggcg gccgtgagcg ccgcaagcag
     2581 cgcgtacgcg agcccgagac ccgtcatcag gtccacgatc gacagcccga agcgggccgg
     2641 cggcgccccg ggttccccgg tgagcgaaaa gtagccagcc tccgcctgca tcaggtaatc
     2701 gaatcccggc cagtcggcgc gcgggccgct tctgccgtag gcggaaaggt gcgcgcagac
     2761 gattgccggg ttgtgctctc tcaggcggtc atacacgagg cccagcttcg ccggcacatc
     2821 cccgcgcaga ttggaggcaa ccgcatctgc gccacgcacg agatcgtgca gcacggcctg
     2881 cccttccggg cgcgcaagat cgagcgtgag gcttttcttg ttgcgattga agctgtggaa
     2941 gaactggctg tcgccggagg cgaagaagtg cggccccact gcgcgcgaca tgtcgccgcc
     3001 gtcgttcggg ttctcgatct tgatcacttc tgcgccctgg tcggccagga acatcgtgcc
     3061 gaacgggccc gccccgtact gctccaccgc cagcacgcgc acgccggcta acggaagcaa
     3121 gaaaaacccc tgacgcaccc gccgatgaac gccgatgaac gccgatttcg gctaaacatc
     3181 acgaaggtct cgtacgcaat cgcaataaaa cgtcgtcatt tcttggcggc cttggcgtct
     3241 tggcggctaa tttacgcggg attccgcttg acgagctcgc gcgcgatgat gatcctctgc
     3301 agctcgttgg tgccctcgcc gatcgcaagc agcggcgcgt cgcggtaata gcgctcgagg
     3361 ttgaactcct tcgagtagcc gtaggcgccg tggatgcgca tcgcctccag gctgttctcc
     3421 agcgccgcct cggtggcata gagcttggcc atgcccgctt cgaggtcgca tcggcgcccc
     3481 gcgtcgtagg cgcgcgcggc ctgctcgacc agcaggcgcg aggcttccac ccgcgtcgcc
     3541 atgtcggcga gcttcaactg gatcgcctgg tgctcggcaa tcggcttccc gaagctcttg
     3601 cgcaactggc cgtagcgcag cgactcctcc aggcaggcgc gcgcgaggcc cgcgccgcgc
     3661 gcggcaacgt tgatgcgccc gagctcgagc ccgttcagca cctgctgcaa tccgcggccc
     3721 tcctgcgttc cgatcaggtt caccgccggc acgcgacagt cctcgaacag cagttccgcg
     3781 gtgtcgatgc cgcggtagcc cagcttctcg agcttgcgca ccaccttgaa acccggcccc
     3841 ttttcgatca gcagcaggct catgcccttg tgcggcggct gcgccttcga atcggtcttc
     3901 accagaaccg ccaggaggtg gccctcggcg ctgttggtga tccaggtctt ggccccgttg
     3961 accacgtagt gatcgccctc gcggcgcgcc tgggtgcgga ccgcctggag atcggtgccg
     4021 cagtccggct cggtcagcgc gatgccgccg cgcagctcgc cgctcgcgaa tcgcggaagg
     4081 tagcgtgcct tctgctccgc cgtgccatgg cgctgcaccg cgcccgccat gatgaggtgt
     4141 gaattgaaca gccccgggac cgacatccac accgccgaca tgcgctcgac gatcttcgcg
     4201 taggtcaccg ccgtcagccc gagaccgccg tgctcgggcg cgatggtggc gccgaacatc
     4261 ccgagcgtcc taagcttctc gacgatcgcc gtcgggtagg tgtcggcggc ttccagcgcg
     4321 cgcacgaccg gccgcacctc ccgctcgagg aagcggtcca cgctctgcag gatgagcgcg
     4381 tcctgcccgt cgctcgttgt cattaaatgc aaccgccgat gaacgccgat gaacgccgat
     4441 gaagactttt gctaaacaat gtgatggcct tcatattgat cttgaattta tcggcgtgaa
     4501 tcggcgttca tcggcggcaa atattttcag tagccggcga gatcgtccgc cgcgtggccg
     4561 cgcctgggca ccaggatcga gcgctcgaaa ttcatgatcg cggtgccatc ggccttcctg
     4621 ccggtcgtgc gcacggtaac gatcccctgc gtgggccgcg atttcgactc gcgcaccgcc
     4681 agcacctcgg actcggcata gatcgtatcg ccggcgaaga ccggcgccgt gagggtgacc
     4741 ttgtcccagc cgagattcgc gaccgccttc tggctcaaat cgctcacgct catgccggtg
     4801 accatcgcga gcgtcaggca actgttgacc aggggtcgcc cgaattcgga cttcgcggca
     4861 tacgccgcgt cgaagtgcag cgggtgggtg ttcatggtga gcagcgtgaa ccaggtgttg
     4921 tcggcttcgc tgatcgtgcg cccgggccgg tgctcgtaga cgtcgccgac cttgaaatcc
     4981 tcgaaatagc ggccgaagga ctcgcgataa cggttcggcc cgatgcgctt gcgctcaacc
     5041 gtcattttga aaatagacag gatttacaag attaataaga ttaacaagat tagaacgaac
     5101 caaattcaag atccggcttt tgttgttgtt gattgaatcc tgcaaatccc gttaatcctg
     5161 tccatttctt gtcgttgacg tcccggcgtt cagcggccgg cggcggagat gcgctggggc
     5221 tgctcgggac gagagagccg cagcaccgcg ccgatatcct tcgcttgttc gagggctagc
     5281 agtgcgccgg cgagctcgtc aagccggcgc gccgaaagat acgggccggc gaggccatcg
     5341 aacttggcgc ggagttcggc cgcggtgaga aaattcgccg gctcgccttt cggcacccgc
     5401 acgtaggact cgaaggcgcc gcgcgcagtc ctgatccgca cgctcccgct catttccgcc
     5461 gggaaatcgg cctgcgcctt cggatcaaca cgggtacgca cccgcttgca cagggccagc
     5521 gtggccggat cgccgagatg gcgcgcgtaa tcgtcccact cgaggccgcc gctgcgcagc
     5581 gccacggcgg cgcagaaggc catcgagaac tgcccgtcga ccaccgactt cggcgactgc
     5641 ttggccggtt ccgggtcgcc ggtgatcttc cagcccggct cgggcagacc gaccgcgacc
     5701 tcctccactt caccggcgtt gatgccgtgc tcgcgggcga gcgcgagaat gccgtcgagg
     5761 ggagcatggg tatagcggca agaaggataa ggcttcaccg cgattttcag cgtttcccag
     5821 cgtcgaccga aaccatccac ggcctttgcc gcgtcggcac tgggtgcgta ggcgtggaag
     5881 aagccccact tgccctcgaa ggcttcggat gcgcccttgt agccctcgcg cgccagcgtc
     5941 gccgcgacca ggccgcacat cgccgcgtga ccgacgtgcg agcgcttggt ccaggcgcca
     6001 ttcacgagaa actgcatcga gcccgccgac atgctgagca cgatgccgaa agcgttggcg
     6061 tagccctccg ggtcgagccc gaggagccgc ccggcggcgg cggcggcgcc gaacgctccg
     6121 caagtcgctg tcgggtggaa accccggtcg tagtgcgcct tcggatcgag cgcgagggaa
     6181 agccggatct gcacctcgta accggcgacg atggcggcga tcagcgcctt gccttcggcg
     6241 cccgtcattt cggcggctgc gaatgcggcc ggcacgatcg gtgcgctcgg atgcaaagaa
     6301 cccgaagcgt gggtgtcgtc gaaatcgagc gaatgggcaa gtgtgccgtt cagcatcgcc
     6361 gcgcccgccg gcgtatagcc ggccacgtcc ccgatcaccg tgcaggcccc attcgccagc
     6421 ccgagatgcc cggcggcggc caccatcgcc ggcgtcgatt ccgcctcgtt gcgggcgcgc
     6481 acggatatgc cggcgagatc gagaacgagc gccttggcac gctcgcggac ttcaaccggc
     6541 agcgcgtcgt actaggcccc tgctacgaat tcggcaatcg cgcgcgtggt cgcagtcatg
     6601 ccgtattccc cggagttttc ccggagcgcg gaaaaattcc tgaatggcgt cgaatcacga
     6661 ctcgcgaatc atgattcacg cctcttcgcg ttcagccggc ctggatcacg ccacaggcga
     6721 ggcgcgcgcc ggcattgccg gtgggctggg tcttgaagtc gtccgggtcg gcgtgaacga
     6781 tgacggcgcg gccgataatg ctggtcgggc cgggcgtcac ggtgatgttg tcgacctcga
     6841 ctttgacgtt ggcgcgcccg gccttgtttg ccttgagcgc aggcaggtca ccggcgtggc
     6901 tctgggcgct gccgggcatg ccgtgaggct tgcctagggg attgaaatgg ccgccggcgc
     6961 tcatgccgtc gcccgagctg cagtccccct tctcgtggat gtgcaggccg tgctcctgtc
     7021 cgggcctgag cccctgaacg aatatgacga cgtgaacctt gctgcctacc tgctcgaaag
     7081 tggcctcgcc aatggtcttg ttgcccttgg tcggcttcaa tcccgcattt gcgcgcggcg
     7141 cttcggtggg tgtcgtctga caggcgccca acaggacggc gacagctgcg gttgcggcaa
     7201 taatgagtgg tttcatggtt tctcctcctc tggaccctca atttactcaa ttcctcatca
     7261 gcttggaagc ccccgctcaa cacccttgtt tttcggtggt tagcggccgg ctgttgcgaa
     7321 gcaatgagat aatggagtca cgttcccccg ctttccattc tttattcaga aacagcccat
     7381 gagacaagct gccgcgatac ctgcacccgc gctggccggg tgcccgcagg cgcccgaggg
     7441 cgccgatctc atcgagtggt attacgaacg cggctactcc gacggctttc cgctggttcc
     7501 acccacgccg gagaagatcg cggcggtcgt tgacaacctc ggcggcgacg ccgggcgcct
     7561 ggtgagccgc gtgccgccgc gctggggcag tctcacgcgc gaagtgctgg cgatcaacat
     7621 ggtgatggcc ggctgccggc ccgaatacgc gccggtggtc gaggccgctc tgctcgcgtt
     7681 atgcgacacg cgcttcaacc tgaacggcgt gcaggccacg acccacatgg caagcccgct
     7741 gctggtggtg aacggtcccg cccgccacgc catcgggatg aatgcgggct gcaacgtgtt
     7801 cggctccggc aatcgcgcca acgccacgat cggccgcgcg ctgcgcctcg tgctgctcaa
     7861 tgtcggcggc ggctggccgg ggctgctcga caagagcacg ctgggccacc ccggcaagta
     7921 cacctactgc atcgccgaga acgaggaagc
//
LOCUS       DBNGSG010000132         1638 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3950757, whole genome shotgun sequence.
ACCESSION   DBNGSG010000132 DBNGSG010000000
VERSION     DBNGSG010000132.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3950757"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..845
                     /locus_tag="AC158Q_03445"
     CDS             66..845
                     /locus_tag="AC158Q_03445"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3338853.1"
                     /translation="MADSYSQTAKGKAEFAAKPGKLTVGLKTLLGLVGDKATVADLKL
                     KLPQVPLDKLMPALDRLVADGYLQRAAPPQRAHAESSLDFTRFINRPVKEPTLQQKRN
                     AEATIAGIRPSKTAGYHVSIINRPPKRIAPNAGGDKHTILLIDADDANALVMTRALLL
                     AKFDTRTAGKREEIIAELNKKPPADAIAMDVALPDVGGLELLGRLRDHPTYKTVPIIV
                     MTATTERDDVVAALSYGASGYMSKPFKPEALVESVKAALGL"
     gene            complement(945..1346)
                     /locus_tag="AC158Q_03450"
     CDS             complement(945..1346)
                     /locus_tag="AC158Q_03450"
                     /inference="COORDINATES: protein motif:HMM:NF013228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR3338854.1"
                     /translation="MAEKRFINPEGLMRPGAYTPAVVTSGGRTIYVAGQVSQNEKGEV
                     VGKGDLAAQAEQVYRNLGLALKGAGASFNDLVKINVYVVNFRPEHRDLMSQVRARHVN
                     KDNPPASTLVGVQSLAKPEFLVEVEAVAVID"
     gene            complement(1457..>1638)
                     /locus_tag="AC158Q_03455"
     CDS             complement(1457..>1638)
                     /locus_tag="AC158Q_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007738414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR3338855.1"
                     /translation="SVRITRSRMAGEPADAVIHPRLGGIGLLEYHRGIEAIAEGRAAA
                     ELAIPQIRHLLQDWT"
BASE COUNT          307 a          569 c          498 g          264 t
ORIGIN      
        1 ctcgaaatcg aacggacgtc acgacgccgt tcatttctcg ccgacaacac gagtgcgctg
       61 gatttatggc cgacagctac agccagaccg ccaaaggcaa ggccgaattc gcggccaagc
      121 ccggcaagct cacggtcggg ctgaagacct tgctcggcct ggtcggcgac aaggcaaccg
      181 tcgcggactt gaagctcaag ctgccgcagg tgccgctgga caagctgatg ccggcgctcg
      241 acaggctggt cgccgacggc tacctccagc gcgcggcccc gccgcagcgc gcgcacgccg
      301 aatccagcct tgacttcacg cgcttcatca accggccggt gaaagagccg acgctgcagc
      361 aaaaacggaa cgcggaggcg acgatcgccg gaatccgccc gtcgaagaca gcaggctatc
      421 acgtcagcat catcaaccgt ccccccaagc gcattgcgcc taacgccggc ggcgacaagc
      481 atacgatcct cctcatcgac gccgacgacg ccaacgcgct ggtaatgacg cgcgcgctgc
      541 tgctcgccaa gttcgacacc cgcaccgccg ggaaacggga agaaatcatc gccgaactga
      601 acaagaagcc gccggccgat gcgatcgcca tggacgtggc gctgcccgat gtcggcggcc
      661 tggagctgct cgggcggctg cgcgatcacc cgacctacaa gacggtgccg atcatcgtca
      721 tgaccgcaac gaccgaacgc gacgatgtgg tggccgcgct ctcctacggc gcaagcggct
      781 acatgagcaa gccgttcaag cccgaagcgc tggtggagag cgtgaaggcc gcgctcggcc
      841 tctgagcggc ccgcgagcgt tgggtgtcga gtgtcgggtg tcgggtgtcg agtggaaacg
      901 tcttaccccc tcgacactca atcctcgaca ctttctatcc gccgtcagtc aatgacggcg
      961 accgcctcca cttcgaccag gaactccggc ttggccaggg actgcacgcc caccagcgtg
     1021 ctggcgggcg gattgtcctt gttcacgtgg cgcgcgcgca cctgcgacat caggtcgcgg
     1081 tgctccggcc ggaagttgac cacgtaaacg ttgatcttca ccaggtcgtt gaacgacgcg
     1141 cccgctccct tcagcgccag gcccaggttg cggtaaacct gctccgcctg cgccgcgagg
     1201 tcgcccttgc cgaccacctc gcccttttcg ttctgggaaa cctgcccggc aacatagatc
     1261 gtcctgcccc cgctcgtgac aacggcaggc gtatacgcgc cggggcgcat gagcccttcc
     1321 gggttgatga agcgcttctc agccatggtg ctcctccctt gaaaataatg gcggattgag
     1381 gggacgctct catcatagtc ccctgcatcg gaggccgttt tggtcatctc gaaacggcca
     1441 cccgggtaac gaagtcttac gtccagtcct gcagcaagtg ccggatctgc ggtatggcca
     1501 gctcggccgc ggcgcggcct tccgcgatcg cctcgatccc gcgatggtac tccagcaagc
     1561 cgatcccgcc gagacgcggg tggatcacgg catccgcggg ctcgccggcc atccggctgc
     1621 gcgtaatgcg cacgctca
//
LOCUS       DBNGSG010000133         4761 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1233515, whole genome shotgun sequence.
ACCESSION   DBNGSG010000133 DBNGSG010000000
VERSION     DBNGSG010000133.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4761)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4761
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1233515"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2898
                     /locus_tag="AC158Q_03460"
     CDS             <1..2898
                     /locus_tag="AC158Q_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019042055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3338856.1"
                     /translation="GARGDRVIHVTPGSQFVALDPEHYQLNPQSADHYAALLPQLSAT
                     VGQIDGILHLYGLGASFGDAAQLLPLERQVDRCTAAAAIVRACEAAGTRTTCWLITAR
                     AATGWARERERQAKDFNLADNMDAALWGFGRTLMNEVSGLMVRLADIEDPAALDTSTY
                     TLARELSHPDAEQEIVLTGSGDRYVPRLRLEPSPALQSGPAADPEPPTLRLGFQFPGQ
                     LRNLRWESHARSALADDELEIEVRATGLNFRDVMYSLGLLSDEAVETGFAGPTLGLEF
                     SGVVLRAGRATRGFSRGDRVVGFGPSSFSNRVTAKAASVSLIPDKISFEAAATIPSVF
                     FTVYYSLHHLARLQEGEKVLIHGAAGGVGIAAIQIAKWSGAEIFATAGSDEKRDFLRL
                     LGADHVLDSRSLAFSDEILSITKGRGVDVVLNSLAGEAINRNLRVLKPFGRFLELGKR
                     DFYENTRIGLRPFRNNISYFGIDADQLMQERPEFTEKLFREMMALFRSNVLHPLPYHR
                     FEAEEIVEAFRFMQQSRHIGKIVITYENGIYPIHTARQAKRALELPADATYLVTGGLG
                     GFGLKTAQWLAAKGARNLVLVSRSGPAAPEARAAVAELEGAGVKVHAAACDITDRKAL
                     AALLAEVAATLPPLRGIVHAAAVIEDGLVRNLTREQIRRVFAPKILGAHYLHQLTLGK
                     ELDFFILYSSATTLFGNPGQGNYVAANAQIEAFAAARRAAGLPALCVRWGAIEDAGFL
                     ARNPQIKEALQGRMGGSALHTSVALDTLEELLLANRSGVGVLELDWKTLSRFLPTASS
                     PKFSELAIHADDGKTEEGDGQDIRRLLAELSPAERTATLIEMLKKEVGEILRLSTDKI
                     DEFRSLYDMGLDSLMGVELATAVEARFSVRLPVMAMSENPTITRLAEKIVAQLMDAGG
                     AEEEVPAADAVADQTRQILIQHAQQVDDEAVKRFAEELNQPVDDKTARMIQ"
     gene            3194..4534
                     /locus_tag="AC158Q_03465"
     CDS             3194..4534
                     /locus_tag="AC158Q_03465"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019042056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR3338857.1"
                     /translation="MSRKGLGGLSSQIKERLIQQALERRLRQADTRPAPFKPESKSTD
                     TPEQYYRFHLHPGYQQLRILNEGAARLGVPSPYFKVHEGSAGVKTRIGGRDYLNYASY
                     NYLGLSGSAAVNQAAKAAIDQYGTSVSASRLVAGERGIHRELERALADAYGADDAIVF
                     VSGHATNVTVIGHLFGPKDLVLHDALIHNSVLQGIQLSGAHRLPFPHNDWAALDAILA
                     DQRRQFERVLIVIEGVYSMDGDFPDLPRFIDVKRKHRAFLMVDEAHSFGVMGETGFGL
                     RQHFGLQAQDVDIWMGTLSKSLSSCGGFIAGETALIEHLKFLAPGFLYSVGMAPATAA
                     AALAALECLRREPWRATTLQQRGVYFRDQAQAAGIDVGSSAGMAIVPAITGNSIKAVR
                     LSHALFERGIHVQPILYPAVPEKAARLRFFMSCEHSEEQIRETVRVLREETLRA"
BASE COUNT          836 a         1634 c         1515 g          776 t
ORIGIN      
        1 ggcgcccgcg gcgaccgcgt gatccacgtc acgccgggca gccagttcgt tgcgcttgac
       61 ccggagcatt accagttgaa cccgcagagc gcggaccact acgccgcgct gctgccgcaa
      121 ttgagcgcca cggtcgggca gatcgacggc atcctgcatc tctacggcct gggcgcgtcg
      181 ttcggcgatg ccgcgcagct gctgccgctc gagcggcagg tcgaccgctg cacggcggcg
      241 gcggccatcg tccgcgcctg cgaggccgcc ggcacccgta ccacctgctg gctgatcacg
      301 gcgcgcgccg cgaccgggtg ggcgcgcgag cgcgagcgcc aggcgaagga tttcaacctg
      361 gccgacaaca tggacgccgc gctgtgggga ttcgggcgca cgttgatgaa cgaagtgtcc
      421 ggcctcatgg tccgcctggc ggacatcgag gatcccgcgg cgctcgatac cagcacctac
      481 acgctggcac gcgagctctc gcacccggac gccgagcagg agatcgtgct gaccggctcc
      541 ggggaccgct acgttccgcg gctgcgcctg gagccgtctc ccgccttgca gtccgggccg
      601 gcggcggacc ccgagccgcc cacgctgcgc ctggggttcc agttcccggg ccagctgcgc
      661 aacctgcgct gggaatcgca cgcgcgctcc gcgctggccg acgacgagct ggagatcgag
      721 gtgcgagcca ccggcctcaa cttccgggac gtcatgtact cgctcggcct gctgtcggac
      781 gaggcggtcg aaaccgggtt tgccgggccg acgctgggcc ttgaattctc cggcgtggtg
      841 ctgcgggcgg gccgggcgac ccgcggcttc tcgcgcggcg accgcgtcgt cggtttcggg
      901 ccctccagtt tcagcaaccg cgtcaccgcc aaggcggcca gcgtgtcgct catcccggac
      961 aagatttcgt tcgaggcagc cgcgacgatc cccagcgtct tcttcacggt ctattattcg
     1021 ctgcaccatc tcgcgcgcct tcaggagggc gagaaagtcc tcatccacgg cgccgccggc
     1081 ggcgtcggca tcgcggcgat ccagatcgcc aagtggagcg gcgccgagat cttcgccacc
     1141 gccggttcgg acgagaaacg cgacttcctg cgcctgctcg gcgcggacca cgtgctcgac
     1201 tcgcgctcgc tcgccttcag cgacgagatc ctctcgatca ccaagggccg cggcgtggac
     1261 gtggtgctga actcgctggc gggcgaggcg atcaaccgca acctgcgcgt gttgaagccg
     1321 ttcggccgct tcctcgagct cggcaagcgc gatttctacg agaacacgcg catcgggctg
     1381 cgcccgttcc gcaacaacat cagctatttc ggcatcgacg ccgaccagtt gatgcaggag
     1441 cgcccggagt tcaccgagaa gctgttccgc gagatgatgg cgctgttcag gagcaacgtc
     1501 ctgcacccgc tgccgtatca ccgcttcgag gccgaggaaa tcgtcgaagc gttccgcttc
     1561 atgcagcagt cgcgccacat cggcaagatc gtcatcacct acgagaacgg catctacccc
     1621 atccacaccg cgcgccaggc gaagcgcgcc ctggagctgc ccgcggacgc gacttacctc
     1681 gtcaccggcg gcctgggcgg cttcggcctg aagaccgcgc agtggctcgc ggccaagggc
     1741 gcgcgcaacc tggtgctggt gagccgcagc ggccccgccg cgcccgaggc ccgcgccgcg
     1801 gtcgccgagc tggagggcgc cggggtcaag gtgcacgccg ccgcctgcga catcaccgac
     1861 cgcaaggcgc tggcggcgct gctggcggaa gtcgccgcca ccctgccgcc gctgcgcggc
     1921 atcgtgcacg cggccgcggt catcgaggac ggcctggtgc gcaacctgac gcgcgagcag
     1981 atccgccgcg ttttcgcccc gaagatcctg ggcgcccatt acctgcacca gctgacgctg
     2041 ggcaaggagc tggatttctt cattctctat tcctccgcga ccacgctgtt cggcaacccc
     2101 gggcagggca attacgtcgc ggcgaacgcg cagatcgagg cgttcgcggc cgcgcgccgc
     2161 gcggccgggc tgccggcgct gtgcgtgcgc tggggcgcca tcgaggacgc cgggttcctc
     2221 gcgcgcaacc cgcagatcaa ggaagcgctc cagggccgca tgggcggctc cgcgctgcac
     2281 accagcgtcg cgctcgacac gctggaggag ctgctgctcg cgaaccgctc cggcgtgggc
     2341 gtgctcgagc tcgactggaa gacgctctcc cgcttcctgc cgaccgcgag ctcgcccaag
     2401 ttctccgagc tggcgatcca cgcggacgac ggcaagaccg aggagggcga cgggcaggac
     2461 atccggcgcc tgctggcgga gctctcgccg gccgagcgca cggcgaccct gatcgagatg
     2521 ctcaagaagg aagtcgggga gatcctgcgg ctctcgaccg acaagatcga cgagttccgc
     2581 tcgctctacg acatgggcct cgactcgctg atgggcgtcg agctcgcgac cgcggtggaa
     2641 gcgcgcttca gcgtgcggct gccggtcatg gcgatgagcg agaaccccac catcacccgg
     2701 ctcgcggaga agatcgtcgc ccagctcatg gatgccggcg gcgcggagga agaggtcccg
     2761 gccgcggacg ccgtcgccga ccagacgcgc cagatcctga tccagcacgc gcagcaggtg
     2821 gacgacgagg cggtgaagcg cttcgccgag gagctgaacc agccggtgga cgacaagacc
     2881 gcgcgcatga tccagtagac aggattgacg ggattcaccg aaaacccgtc aatatcgcaa
     2941 atggacagga ttaacagact tcaatcagat acaccgcaca cccgccaaaa acaaaattag
     3001 acaggattaa caggattaac aggattcaaa caacaaaccc ataagagcag tttgattggt
     3061 ttttttgatc taatcctgta aatcttgtta atcctgtcta ttgatgcttt tgacgggtgt
     3121 tgaatcctgt taatcctgtc tattaatgct cttgacgggt ttttgaatca tttcgtgaat
     3181 cctgtccatt aacatgtcgc gcaagggcct cggcggtctc agctcgcaga tcaaggagcg
     3241 cctgatccag caggcgctcg agcggcggct gcgccaggcc gacacgcggc ccgccccgtt
     3301 caagcctgag tcgaagtcca cggacacgcc cgagcagtac taccgcttcc atctgcatcc
     3361 gggataccag caactgcgca tcctgaacga gggcgccgcc cggctcggcg tccccagccc
     3421 gtacttcaag gtgcacgagg gcagcgccgg ggtgaaaacg cggatcggcg gcagggatta
     3481 tctcaactac gccagctaca actacctcgg gctctccggc agcgccgccg tcaaccaggc
     3541 ggccaaggcg gcgatcgacc agtacggcac ctcggtttcc gcgagccgcc tcgtcgccgg
     3601 cgagcgcggc atccaccgcg agctggagcg ggcgctcgcc gacgcctacg gcgcggacga
     3661 cgccattgtc ttcgtcagcg gccacgcgac caacgtcacc gtcatcggcc acctgttcgg
     3721 gccgaaggac ctcgtcctgc acgatgcgct gatccacaac agcgtgctgc agggcatcca
     3781 gctctcgggc gcccaccgcc tgccgtttcc ccacaacgac tgggccgcgc tggatgcgat
     3841 cctcgccgac cagcggcgcc agttcgagcg cgtgctgatc gtcatcgagg gcgtctacag
     3901 catggatggc gactttcccg acctgccgcg cttcatcgac gtcaagcgca agcatcgcgc
     3961 cttcctgatg gtggacgaag cccactcctt cggcgtcatg ggcgagaccg gcttcgggct
     4021 gcgacagcac ttcggcctcc aggcccagga cgtggacatc tggatgggaa cgctcagcaa
     4081 gtcgctgtcg agctgcggcg gcttcatcgc cggcgagacc gcgctgatcg agcacctgaa
     4141 gttcctggcg ccgggctttc tctacagcgt cggcatggcg cccgcgaccg ccgccgccgc
     4201 gctggccgcg ctcgaatgcc tgcgtcgcga gccctggcgc gccacgaccc tgcagcagcg
     4261 gggcgtctac ttccgggacc aggcgcaggc cgccggaatc gacgtggggt cgagcgcggg
     4321 catggcgatc gtcccggcca tcaccggcaa ttcgatcaag gcggtgcggc tgtcacacgc
     4381 gctgttcgag cgcggcatcc acgtgcagcc gatactctac cccgcggtcc cggaaaaagc
     4441 cgcgcggttg cgcttcttca tgagctgcga gcactcggag gagcagatcc gggagaccgt
     4501 gcgcgtcctg cgcgaggaaa ccctccgcgc ctaatagaca ggattaacag acttcaatcg
     4561 atacaccgca cacccgccaa aaacacaaat agacaggatt aacaggatta acaagattca
     4621 aacagcaaca tcaaaagaag cgttctgatt tgttcttgat ctagtcctgc aaatcctgtt
     4681 catcctgtcc attacggttc tggcaggttt gtttgaattg tttttttgtt ctaatcatgt
     4741 aaatcctgtt aatcctgtcc a
//
LOCUS       DBNGSG010000134         4144 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2593362, whole genome shotgun sequence.
ACCESSION   DBNGSG010000134 DBNGSG010000000
VERSION     DBNGSG010000134.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4144)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4144)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4144
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2593362"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="AC158Q_03470"
     CDS             <1..288
                     /locus_tag="AC158Q_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008350171.1"
                     /note="catalyzes the thiolytic cleavage of
                     beta-ketoadipyl-CoA to succinate and acetyl-CoA; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HIR3338858.1"
                     /translation="LARAGLTMKDLDIIEINEAFAAQVLACLKGLGVAFDDTRVNPNG
                     GAIAVGHPLGASGARLALTAARQLQRSGGRYALVSLCIGGGQGMAAILERV"
     gene            671..1984
                     /locus_tag="AC158Q_03475"
     CDS             671..1984
                     /locus_tag="AC158Q_03475"
                     /EC_number="2.7.7.-"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR3338859.1"
                     /translation="MDEIASRLTPGEAPNSPVPASNSAVGSAQVISHPAAATKQPLVV
                     TVDQMPHPAYMVNYRFEVVWFNDKAREALGGALSQIAARTEERSIFRLLLANEDAKSF
                     SSREELLRLNIALAKERMELAAISQPLQPIAAANNQLLERLDAETSPVPAAPIVELPV
                     RLKEISGNRVADYCAFASYFREGILVILVPLGEDANGLLQLLSRRDSVIRNLLSKRLP
                     TLTDLAVLVADLQESVKICSELPPEEYFELINQIWSAAGAVFRKYYGTHGKHVGDGMV
                     YYFFPQPDCSYIMNAVVCAQELKGVMSRISKDWQLRKKWTTELYLNTALNEGQEWLGT
                     FQTATTVEFAVLGDTINHTARLSDFARHGTVWATKNFLGRIPANERPRVKFGIHRAGP
                     DGQHTFVESTYSRLGNLIDLSAGRYEKLKDISGLPVTEIVDVRGS"
     gene            2306..3139
                     /locus_tag="AC158Q_03480"
     CDS             2306..3139
                     /locus_tag="AC158Q_03480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIR3338860.1"
                     /translation="MTLYDWVADSLLGKASHAWVRYLAAGLVTLLSIFIVAVLWRRMG
                     AIPDPGLILLLTVAAATYLAGGMAGMLSAAIVLFCSFVLFSHPLYPFRYSEMDWRQMM
                     VIIVACPIIALMVGSLKEQVDRLKIVTREAQALKEEVQRLEVVRESQFLCEQRFRLMA
                     DCMPDYAVCVLDANGSVRNWNPGAERLLGYSDPEIVGQSYSRFFAKEDLLARQPERLL
                     EHARFSGRVVEEGWRLRKGGGRIRATTTLLALKNLQGVPAGCLMVMRATLDSPDITHK
                     S"
     gene            complement(3562..>4144)
                     /locus_tag="AC158Q_03485"
     CDS             complement(3562..>4144)
                     /locus_tag="AC158Q_03485"
                     /inference="COORDINATES: protein motif:HMM:NF024206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR3338861.1"
                     /translation="AQADSYEAVLHAVNNDDERTVAELIRRGLDVNTVGPNGDSLLML
                     AAKHGRPQMVRAVLDAKPRVYVRNAYGETALMMAVFQGHTEVVRQLLARGAEINHAGW
                     TPLIYAAAQNRMDIARILISRGALVNAQAENGTTALMMAAREGQLPMVLLLMEHGADI
                     NMKDLDGQTALSAAKTRERRDVIEVLVRAGAAE"
BASE COUNT          840 a         1210 c         1228 g          866 t
ORIGIN      
        1 ctggcgcgtg ccgggctcac gatgaaggac ctcgatatca tcgaaatcaa cgaagccttt
       61 gccgcgcagg tgctggcctg cctgaaaggg cttggagtgg cattcgacga tacccgcgtc
      121 aaccccaacg gcggcgccat cgccgtcggg catccgctcg gggcttcagg cgcgcggctc
      181 gcgcttacgg cggcacgcca actgcaacgt tcgggcggac gttacgccct tgtcagcctg
      241 tgtattggcg gcgggcaggg catggcggcg atactcgagc gagtctgatc cctctctgcc
      301 ctgcatgaaa ccggggtgtt taccccggtt tttttcgccc tgtatcaagc cctttgccat
      361 caaggagggg ggcgttgtgt ccttctcgca acaacgagct tgacgaggga gattggtagg
      421 aggaaaatct gtataagcag tctatatagt ctgtttatat acaatgataa caagtaagga
      481 attacttgaa aaaacaggca tttccagggc gacattgaat aattatattg cccggggcct
      541 actgacgaga cccgttgttg cgagtcccga ggtacgaggt gggaatcgac cgaggcaaat
      601 cgggtatttt ccggacgatg ccctggctcg cgtctggcag atcctgcaat tgaaagtgga
      661 ggggttaagc atggatgaga tcgcatcaag actcacacca ggggaagctc ccaattcacc
      721 cgtgccggcc tcgaattcgg ccgtcggcag cgcgcaggtg atttcccacc cggcggcggc
      781 cacgaagcag ccgctggtcg tcaccgtgga ccaaatgccg caccctgctt acatggtcaa
      841 ttaccggttt gaggtggttt ggttcaatga caaggcccgc gaggcgctcg gcggcgcctt
      901 gagccagatc gcagcgcgaa cggaagagcg gagtattttc cggttgctgc tggcgaatga
      961 agacgcgaaa tcattttcga gccgggagga attgcttcgc ctcaatatcg ctctcgcaaa
     1021 ggaacggatg gaattggctg cgattagcca accattgcag ccgatcgcgg cagccaataa
     1081 ccagttgctc gagcggctgg acgccgaaac cagcccggtg cctgcggccc ccattgtcga
     1141 gctgcctgtc cgattaaaag agatctccgg caaccgcgtc gcggattact gcgcctttgc
     1201 ctcgtatttc cgcgagggca tcctagtgat cctggttccg ctcggagagg atgccaacgg
     1261 ccttctgcag cttctcagca ggcgcgattc ggtcattcgg aacctcctca gcaagcgcct
     1321 gcccacgctg acagaccttg cagtgctcgt agccgatttg caggaatccg taaagatctg
     1381 ctcggagctg ccgccggaag aatatttcga gctcatcaac cagatctggt cggccgccgg
     1441 ggcggtattc cggaagtact acggcactca cggcaagcac gtgggcgacg gaatggttta
     1501 ttacttcttc ccgcaacccg actgcagtta catcatgaat gccgtggtat gcgcccagga
     1561 actgaagggc gtgatgtcgc gcatcagcaa ggactggcag ctgcggaaga aatggaccac
     1621 ggagctttac ctgaacacgg cattgaatga agggcaggaa tggctgggaa catttcagac
     1681 cgccaccacc gtggagtttg ccgttctggg ggacacgatc aatcacacgg cgcgcctcag
     1741 cgattttgcc cgtcacggga ccgtctgggc aaccaagaat ttcctcggca gaatcccggc
     1801 gaatgagcgt ccccgcgtca aattcggaat acatcgtgca gggccggacg ggcagcacac
     1861 cttcgtggag tccacctact cgcgcttggg caacttgata gacctcagcg ccggacgcta
     1921 tgagaagctc aaggacattt ccggcctgcc agtaacggaa atcgttgacg tccgaggatc
     1981 atagctccct gcattacgct cacggcaatc gccagacgcg cgatcaagcc gcgcgtcggg
     2041 ggcgggcagg gtgattgcga tgcccgagta tgtttgatag tcgcatggac gctcggtacc
     2101 gccgtttttc cattgcaagt acagcttgat acacccgcat cgacggtcgt gacgtttgtc
     2161 acgtcaccac gcagtcccac tctggtaccc ttcttgcgcc ctaccgggct gatgtagcta
     2221 aagagggctt aaagactctc aacagactga atatgaacgg gttatgacga aaacccgtca
     2281 actgccgtag tgccaggggc cggacatgac tctgtatgac tgggtggcgg attcgctgtt
     2341 gggcaaggcg tcccacgctt gggtgcgcta cctcgccgcg ggactcgtca cgctgctctc
     2401 gattttcatt gtcgccgtgc tgtggcggcg gatgggcgcg attcctgatc cggggctgat
     2461 cctgctgctc acggtcgcgg ccgcaaccta tctcgcaggc ggcatggcag gcatgttgag
     2521 cgcagccatc gtcctgttct gctccttcgt gctgttttct catcctctgt acccgttccg
     2581 ttattcggag atggattggc gacaaatgat ggtgatcatc gtggcctgtc cgatcatcgc
     2641 cctcatggtg ggatcgctca aggaacaagt ggacaggctg aaaattgtca cccgggaggc
     2701 ccaggcgctc aaggaggagg tgcaacgact cgaggtcgtc agggaatccc agttcctgtg
     2761 cgagcagcgg ttccgcctca tggccgattg catgccggat tacgccgtct gcgtgctgga
     2821 cgccaacgga tcggtaagaa actggaaccc gggggcggag cggctgctcg gttattcgga
     2881 cccggagatc gtggggcaaa gctattcgcg attcttcgcc aaggaggatt tgctggcccg
     2941 tcagcccgaa cgattgctcg agcacgcgcg tttctcgggc agggtggtgg aggagggctg
     3001 gcgcctgcgc aagggcgggg ggcgcatccg tgccacgacc actctgctgg ccctgaaaaa
     3061 cttacagggt gtgccggctg gctgcctgat ggtgatgcgc gccaccctgg acagcccgga
     3121 catcactcac aaatcctgag gcgttcagga agcggcgtgg ttggtcttcc cggcggctca
     3181 cgaaggcctc ttatacctcg catcgccctg tagcgccgat cccctgcgct tatccagaaa
     3241 tatcctggcg atgacgctca ccagcaccac gatacagata agtgccagga cagctgcaac
     3301 aaacaaccag aacggctgac accgggctgt ttctccctcg gcgtttacgc aggaatcggc
     3361 atacgccaac catctgccga ttctcatgtc gatcaacctg agcagttcca cgaccagatg
     3421 ccggattcag gcggaataac aaagcagggt agtcaggccg ctaattgcgc ttctcgccat
     3481 tgtcccagtc tcatgtaaag gatcatattt aacataatat acattatacg catatataga
     3541 ggaggctggg ccgtttcaag cctattcggc cgcgccggca cggaccagca cttctatgac
     3601 gtcacggcgc tcccgggttt tcgccgcaga caatgcggtt tggccgtcaa ggtctttcat
     3661 gttgatgtcg gcaccgtgtt ccatcaacaa taggaccatc ggcagctggc cctcacgggc
     3721 agccatcatc agcgccgtcg tcccgttttc agcctgggca ttgaccaggg cgccccggct
     3781 gatcaggatg cgcgcgatat ccatccgatt ctgtgctgcg gcgtaaataa gcggtgtcca
     3841 gcctgcgtga ttgatctcgg cgccccgggc caggagctgg cgcactacct cggtatgtcc
     3901 ctggaacacg gccatcatca gtgcagtttc gccgtaagca ttccgcacat agacccgggg
     3961 tttggcatcc agtaccgctc tcaccatttg cggcctacca tgcttggcgg caagcatcag
     4021 gagagagtct ccgttgggcc cgaccgtatt cacgtcgagt ccgcgcctga tcaattcggc
     4081 caccgttcgc tcatcgtcgt tattgaccgc gtgaagcacg gcctcataac tgtcggcctg
     4141 ggct
//
LOCUS       DBNGSG010000135         1541 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3272831, whole genome shotgun sequence.
ACCESSION   DBNGSG010000135 DBNGSG010000000
VERSION     DBNGSG010000135.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3272831"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="AC158Q_03490"
     CDS             complement(<1..759)
                     /locus_tag="AC158Q_03490"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3338862.1"
                     /translation="MAPDRPACGACGGPMTPFMATRDYNRRVSDTVFDYYRCGRCGLV
                     SLVNVPADLGPFYAADYHALPASASVIERAARHDRYKIELVRRYVSGGRLLEIGPSWG
                     AFCLLAKRAGFEVEAIEMDPRCCEFLEEKIGVRAINSMDAAAALDRAAVPDVIAMWHV
                     IEHLRDPWTLLARLAERLAPGGILVLATPNPEAVQFGLFGRRWTHVDAPRHVHLIPQA
                     LLRGKLGEYGLRELLTTTRDAGSIGWNSFGWSFSA"
     gene            complement(789..>1541)
                     /locus_tag="AC158Q_03495"
     CDS             complement(789..>1541)
                     /locus_tag="AC158Q_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338863.1"
                     /translation="YDQILQDLPHRKMHVADRGFDLKPYFDAVNRLDYRHFCFLNSFS
                     GILEGNWLAKLHRWIIADGVGLVGATGSYESIASNYAARVRMLGEMGPAARLRWKLTH
                     VVSGARPAIAAQRAAGLLVEGLGLKDPRRRFPPFPNYHVRTNAFMAARETLMRVRPGP
                     VFFKLSAYFFESGRDSLTRQVMRLGLRPLVVGRDGAGYEKEQWHLANTFWQGRQENLL
                     VADNRTDLYAAGDAAARAELSRLAWGAHARPA"
BASE COUNT          270 a          542 c          495 g          234 t
ORIGIN      
        1 cgcggaaaag ctccacccga agctgttcca cccgatgctg ccggcatcgc gagtcgtcgt
       61 cagcagctcc cgcaggccgt attccccgag cttcccgcgc agcagcgcct ggggtatgag
      121 gtgaacgtgt cgcggggcat ccacgtgggt ccaccgccgg ccgaacaggc cgaactgcac
      181 cgcctccgga ttcggcgtcg ccaataccag gattccaccg ggcgccaggc gctcggccag
      241 cctggcaagc aaggtccagg gatcgcgcaa gtgctcgatg acatgccaca tcgcgatcac
      301 gtcgggaacg gcggcccggt cgagcgccgc cgccgcgtcc atgctgttga tcgcgcgcac
      361 gccgatcttc tcttccagga actcgcagca gcgcggatcc atctcgatcg cctcgacctc
      421 gaagccggcg cgcttggcga gcaggcagaa cgcgccccac gacggcccga tttccagcag
      481 gcgcccgccg gagacatagc gccgtaccag ctcgatcttg tagcggtcgt gccgcgcggc
      541 gcgctcgatc accgaggcgc tggccggcag ggcgtggtaa tcggcggcgt aaaacgggcc
      601 caggtccgcg ggcacgttca ccagggacac cagcccgcaa cggccgcagc gataatagtc
      661 aaatacggtg tcggaaacgc gccggttgta gtcccgggtc gccatgaacg gcgtcatcgg
      721 tccgccgcac gcgccgcaag ccggcctgtc gggagccatg cttttgtcca taccgggccg
      781 ccggggcctc aggccgggcg cgcgtgcgcg ccccacgcga gccgggacag ttcggcgcgg
      841 gcagccgcat cgcccgccgc gtacaaatcc gtccggttgt ccgcaaccag caggttttcc
      901 tgccggcctt gccagaacgt gttggcgaga tgccattgct ccttctcgta accggcgcca
      961 tcgcgcccca cgaccagcgg gcgcaggccg aggcgcatga cctgccgggt gagactgtcg
     1021 cggccgctct cgaaaaaata cgccgacagc ttgaagaaca ccgggccggg ccgcacgcgc
     1081 atcagcgtct cgcgcgcggc catgaacgcg ttggtgcgga catgataatt cggaaatggc
     1141 gggaagcgcc tcctgggatc cttcaggccc aggccttcca cgagcaggcc cgcggcgcgc
     1201 tgcgcggcga tcgcggggcg cgcgccgctc actacgtgag tcagcttcca gcgcaacctc
     1261 gccgcgggcc ccatttcccc aagcatcctg acgcgtgcgg cgtaattgct tgcaatgctc
     1321 tcgtacgacc cggtcgcacc caccaacccc acgccgtcgg cgattatcca gcgatgaagc
     1381 ttcgcgagcc agttgccctc gaggatgccg ctgaaggagt tcagaaaaca gaaatgccgg
     1441 taatcgagcc ggttgaccgc gtcgaaataa ggcttcaagt cgaaaccacg gtcggcgacg
     1501 tgcattttcc ggtgcggcag gtcctgcagt atctggtcgt a
//
LOCUS       DBNGSG010000136         6234 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_726074, whole genome shotgun sequence.
ACCESSION   DBNGSG010000136 DBNGSG010000000
VERSION     DBNGSG010000136.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6234)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6234)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6234
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_726074"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /locus_tag="AC158Q_03500"
     CDS             <1..987
                     /locus_tag="AC158Q_03500"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010113727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HIR3338864.1"
                     /translation="MKIAVLGAGAWGTAISLALGARHEIRLWARNAALINALRADRAN
                     RRYLPGFTLPGSLALDENFGSALQGAELIVIAVITNGLRETLRRIRATGSGAPVVWLC
                     KGFESGQARLPHQVCAEELAAAAPRAVLSGPSFAEEVARGLPAALTLASTDGGFAREA
                     ARALHGPTLRIYPSEDVIGVEAAGAVKNVMAIAAGVSDGLELGLSARAALITRGLAEM
                     TRLGVALGGRAETFMGLAGAGDLILTCTGDLSRNRRVGLALARGAALADVLSGLGHTA
                     EGVLTARAVANLARERGVEMPITRAVCRVLDDPRQARAAVQELLAREQTTEY"
     gene            complement(1100..1564)
                     /gene="trmL"
                     /locus_tag="AC158Q_03505"
     CDS             complement(1100..1564)
                     /gene="trmL"
                     /locus_tag="AC158Q_03505"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HIR3338865.1"
                     /translation="MLDIVLFEPEIPPNTGNAIRLCANAGCRLHLVRPLGFSLRDRQL
                     QRAGLDYHAFTQVLQHDDWRACRASLAGRRLFAVTTRGTRRYDEPSYGENDVFLFGPE
                     TRGLPEEVLAEFVPEQRIRVPMLAQKRSLNLSNAVAVVVYEAWRQLGFRGGC"
     gene            1662..3368
                     /locus_tag="AC158Q_03510"
     CDS             1662..3368
                     /locus_tag="AC158Q_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HIR3338866.1"
                     /translation="MGRPEKIRLGEILIQQNLLTEEQLKAALEEQKKSGRRLGRVVIE
                     KGYVTEEQISQALARQLNVPYISLKQFNIKRDVTLKLPETQARRYRALVLEDAGRTYK
                     VGMADPTDLVAYDEIARVLKRDIELAVITESELLRTIDRIYRRTEQITGLAHELEAEV
                     GDAAVVDFGALALTQGLEEAPVVKLLQTVFEDAIAARASDIHVEPQEKRLHIRFRIDG
                     VLHLQTEADAKIASAVVLRLKLMSGLDISEKRLPQDGRFNIKVRNAQIDVRISTMPVQ
                     HGESVVMRLLHRSSGVLGLDRIGMPEAMLARLREVIHRPSGMVLVTGPTGSGKTTTLY
                     SALGELNTHERKIVTVEDPVEYRLQGVNQVQVNEKIELTFARVLRSALRQDPDVILVG
                     EMRDPETVETGLRAAMTGHMVFSTLHTNDAVSTPVRLLDMGAPRYMVALSLHLVIAQR
                     LVRVICESCIMDHALLPNEHEWLRQELGDKVDGHRYRRGKGCTHCNGTGYLGRTGIYE
                     MLEMTGPVVEAANQDDARQFIRVAREHMGEHTLRRHAALLVAAGTTTAVEAMRISNQF
                     ED"
     gene            3376..4605
                     /locus_tag="AC158Q_03515"
     CDS             3376..4605
                     /locus_tag="AC158Q_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR3338867.1"
                     /translation="MPHFAYKGRDGHGQSVEGVLEGADSSAVAGQLFGIGVSPVEITP
                     SSAPLRAGPGLLARLNEQKIGNTEVLLFCRHMYTLLKAGVPIMGALKGLQESTENKAF
                     SRVVQDLRENLDSGHELSVCLTRHPRVFDPFYVAMVRVGELTGTLEEVFLRLFNHLEF
                     EQFMRDQVKSAIRYPAFVVTAMAMAIVIINIFVIPAFAKIYQGFKTELPLMTKMLIGF
                     SNFMVAWWPVMLAALAAAVYGFHLWKTTPRGRLAWDEFKMKIPVAGPIVLKATLARFS
                     RSFALAIRSGVPAVQALTMVANTVDNVYVARRIEAMREGVERGESVLKTAVATGIFTP
                     IVLQMVAVGEETGALDDLMSEIADMYQREVEYDLKSLASQIEPILIVVLGALVLILAL
                     GVFLPIWDLGSAALGKK"
     gene            4698..5180
                     /locus_tag="AC158Q_03520"
     CDS             4698..5180
                     /locus_tag="AC158Q_03520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HIR3338868.1"
                     /translation="MTLFEFAVCVMIIGVLAAVLLNRLVYYQEMAEKAAMESTARLLK
                     TGLQIRLAELIITNRQSEAAALEQENPVQWLETRPSNYGGLIHDDSRPGAWYFDERER
                     QLVYVVNTGNRLDLGGETGPKQVRYSARLLRDRVRTAGGVVESVTGVTLVPVLPYRWP
                     "
     gene            5263..5763
                     /locus_tag="AC158Q_03525"
     CDS             5263..5763
                     /locus_tag="AC158Q_03525"
                     /inference="COORDINATES: protein motif:HMM:NF019575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HIR3338869.1"
                     /translation="MTASSSAKGFTLIELIVVIIILSILAATALPRFANLQVQARVAK
                     LNGAMGSMKGASSLAHAGCLASTPQCTTPPSNTGTLDMEGVPVTMIHAYPTADSAGIV
                     TAAGISAGPASTDGYDVQGGGSSAGSVLRLMVDGNDPVNCSFTYTAPNGPNQSPVFSA
                     LVVTGC"
     gene            5840..>6234
                     /locus_tag="AC158Q_03530"
     CDS             5840..>6234
                     /locus_tag="AC158Q_03530"
                     /inference="COORDINATES: protein motif:HMM:NF019575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HIR3338870.1"
                     /translation="MKTQQGFTLVELIVVIVILGILAATALPRFINVTTDARIAAVEG
                     IAGGLRGAVGLAQSKYFAVGSSAMTTVDMNGTSVTVAAVSGIPTGDAAGIGTAAQLGG
                     VTANYAVTPNTFRPSGGSATCEARYAPSTG"
BASE COUNT         1122 a         1931 c         2104 g         1077 t
ORIGIN      
        1 atgaaaatag cggtactcgg cgcaggcgcc tggggcacgg cgatcagcct tgccctgggt
       61 gcgcgccatg aaatacggct gtgggcgcgc aatgccgcgc tgatcaacgc gttgcgcgcc
      121 gaccgcgcga accggcgtta tctccccggt ttcaccctgc cgggatcgct cgcgctcgac
      181 gaaaatttcg ggtcggcgct gcagggcgcc gagttaatag tcattgcggt catcaccaac
      241 ggcttgcgcg agacgctgcg gcgcatccgc gcgacggggt ccggcgcccc ggtcgtctgg
      301 ctgtgcaagg gattcgaaag cgggcaggcg cgactgccgc atcaggtttg cgcggaagaa
      361 ctggcggccg ctgcgccgcg cgccgtgctg tcgggtccga gctttgcgga agaagtggcg
      421 agggggttgc ctgctgccct cactttggcg tccaccgacg gcggtttcgc gcgcgaagcg
      481 gcacgcgccc tgcacggccc gacattgcgc atttacccga gcgaggatgt gatcggcgtg
      541 gaggcggcgg gtgcggtcaa gaacgtgatg gcgatcgccg ccggcgtgag cgacgggctc
      601 gaactggggc tttccgcgcg cgccgcgctg attacgcgcg ggcttgccga gatgacgcgg
      661 ctcggcgtgg ccctcggcgg gcgcgcggag accttcatgg gactcgccgg cgccggcgac
      721 ctgattctga cctgcaccgg cgatctctcc cgcaaccggc gcgtgggtct cgcgcttgcg
      781 cgcggggccg cgctcgcgga cgtgctctcc gggctcgggc acacggcgga aggcgtgctc
      841 acggcgcgcg cggtagccaa tctcgcgcgc gaacggggag tcgagatgcc gatcacccgc
      901 gcggtgtgcc gcgtgctcga cgacccccgg caggcacgcg ccgcggtgca ggaactgctc
      961 gcacgcgagc agacgaccga gtactagtct cggagcggag tgttaagtgt tgagtgttta
     1021 gttttgagtt gaatgcgggc tccaaacaac tcaacactca acactcaaca ctcaaaactc
     1081 aacattgttt tcgcggcggc tagcagccgc cgcgaaaacc gagctggcgc caggcttcgt
     1141 aaacaaccac ggcgaccgcg ttcgagagat taaggctgcg cttctgcgcg agcataggca
     1201 cgcggatgcg ttgctccggg acgaactccg ccagaacctc ctccggcagg ccccgggttt
     1261 ccgggccgaa aaggaacaca tcgttctcgc catagctcgg ttcatcgtag cggcgcgtgc
     1321 cgcgtgtcgt gacggcgaac agccgccggc cggcgagcga tgcacggcat gccctccagt
     1381 cgtcgtgctg caggacctgc gtaaacgcgt ggtaatcgag tcccgctcgc tgcaattgcc
     1441 tgtcgcgcaa cgagaatccc agcgggcgta ccaggtgcag gcggcatccg gcgttcgcgc
     1501 acaggcgtat tgcattgccg gtgttgggcg gtatttccgg ctcgaacagc acgatatcaa
     1561 gcacgggcag gttgctgccg ggatgaaaac aagataccct tacagatcaa tgagatgtct
     1621 cacgatgttg acagtgtatc caggaccaag ggggggcggc tatgggccgg cctgagaaaa
     1681 tccgcctcgg cgagatacta atacagcaga acctgctcac cgaggagcag ttgaaggccg
     1741 ccctcgagga gcagaagaaa tccggccgcc gcctggggcg ggtggtgatc gagaagggct
     1801 atgtcaccga agagcagatc tcccaggcgc tcgcccgcca gctcaacgtc ccttacatca
     1861 gcctcaagca gttcaacatc aagcgcgatg tgacgctcaa gctgccggaa acccaggcgc
     1921 gccgctaccg agcgttggtg ctggaggacg ccggacggac ttacaaggtc ggcatggccg
     1981 atcccaccga tctcgtggcc tacgacgaaa ttgcccgggt gctgaagcgc gatatcgagc
     2041 tcgcggtgat cacggaatcc gagctgctgc gcaccatcga tcgcatctac cggcgcaccg
     2101 agcagatcac gggccttgcc cacgagctcg aagcggaagt gggcgacgcc gcggttgtgg
     2161 acttcggcgc cctggcgctg acgcaggggc tcgaggaagc cccggtcgtg aagctgctgc
     2221 agaccgtatt cgaggacgcg atcgcggcgc gcgcctccga catccacgtc gagccgcagg
     2281 aaaagcgcct gcacatccgc tttcgcatcg acggcgtgct gcacctgcag accgaagcgg
     2341 atgccaagat cgcctccgcc gtggtgctgc gcctgaagct gatgtcgggc ctcgacatat
     2401 ccgagaagcg gctgccgcag gacgggcgct tcaacatcaa ggtgcgcaac gcccaaatcg
     2461 acgtgcggat ttccaccatg ccggtccagc atggcgagtc ggtggtgatg cggctgctcc
     2521 accggtcgag cggcgtgctg gggcttgacc ggatcggcat gccggaggcg atgctcgccc
     2581 ggctgcgcga agtgatccac cgccccagcg gcatggtgct ggtgacgggg cccaccggca
     2641 gcggcaagac cacgacgctc tattcggcgc tcggcgagct gaacacccac gagcgcaaga
     2701 tcgtgaccgt ggaagacccg gtggagtacc gcctgcaagg cgtcaaccag gtgcaggtca
     2761 acgagaagat cgagctcacc ttcgcccgcg ttctgcgctc cgccttgcgc caggatccgg
     2821 atgtcatcct ggtcggcgag atgcgcgacc cggaaacggt cgagaccggc ctgcgcgcgg
     2881 cgatgaccgg gcacatggtg ttctccacgc tccacaccaa cgacgccgta tccaccccgg
     2941 tgcggctgct cgacatgggc gcgccacgct acatggtggc gctgtcgctg cacctggtga
     3001 tcgcgcagcg gctggtgcgc gtcatctgcg agagctgcat catggaccac gcgctgctgc
     3061 cgaacgagca cgaatggctg cgccaggaac tgggcgacaa ggtggacgga catcgctacc
     3121 ggcgcggcaa gggctgcacg cactgcaacg gcaccggcta cctgggccgc accggcatct
     3181 acgagatgct ggagatgacg ggcccggtgg tcgaggcggc gaaccaggac gatgcccggc
     3241 aattcatccg cgttgcgcgc gagcacatgg gcgagcacac cctgcgccgc cacgccgcgc
     3301 tgctcgtcgc tgccggcacg acgaccgccg tggaggcgat gcgcatcagc aatcagttcg
     3361 aggactagcg cgcgcatgcc gcatttcgcg tataagggcc gcgacgggca cgggcaatcg
     3421 gtcgagggcg tgctggaagg tgccgacagc agcgcggtgg cgggccagct gttcggaatc
     3481 ggcgtctctc ccgttgaaat cacgccgtcg tcggcaccgc tgcgcgccgg cccgggactg
     3541 ctggcgcggc ttaacgagca gaagatcggg aataccgaag tcctgttgtt ctgccgccac
     3601 atgtacacgc tgctcaaggc gggcgtgccc atcatgggcg cgctgaaggg cctgcaggag
     3661 tccaccgaga acaaggcgtt ttcgcgggtg gtgcaggatc tgcgcgagaa cctcgacagc
     3721 gggcacgagc tgtccgtctg cctcacgcgc caccccaggg tattcgaccc gttctacgtg
     3781 gcgatggtgc gcgtgggaga gctcaccggg acgctggaag aggtgttcct gcgcctgttc
     3841 aaccacctcg agttcgagca gttcatgcgc gaccaggtga agtccgcgat ccgctacccg
     3901 gcattcgtgg tgaccgcgat ggcgatggcg atcgtgatca tcaatatctt cgtgattccc
     3961 gcattcgcca agatctacca gggattcaag accgagctgc ccctgatgac caagatgctg
     4021 atcggtttct ccaatttcat ggtggcgtgg tggccggtga tgctggccgc gctggccgcc
     4081 gcggtttacg ggtttcacct gtggaagacg accccccgcg ggcggctcgc ctgggacgag
     4141 ttcaagatga aaatcccggt cgcggggccg atcgtgctga aggccacgct cgcgcgcttt
     4201 tcgcgcagct tcgcgctcgc gatccgcagc ggcgtgccgg cggtgcaggc gctcaccatg
     4261 gtcgcgaaca ccgtggacaa tgtttacgtg gcgcgcagga tcgaggcgat gcgagagggc
     4321 gtggagcgcg gcgaatcggt gctgaagacc gcggtggcga ccggcatctt cacgccgatc
     4381 gtgctgcaga tggtggcggt gggcgaggag accggcgcgc tcgacgatct catgtcggaa
     4441 atcgcggata tgtaccagcg cgaggtggaa tacgatctca agtcgctcgc ctcgcagatc
     4501 gagccgatac tgatcgtggt cctgggcgcg ctggtgctga tcctggcgct cggcgtgttc
     4561 ctgccgatct gggacctcgg cagcgctgcg ctcggcaaga aatgacgcag ttccgagttc
     4621 cgagtttcca gtttccagtt aaacgtgttg tcaactcgca actggtaact cgaaacttga
     4681 aaccggggca gcgcggcatg acccttttcg agtttgccgt ctgcgtcatg atcatcgggg
     4741 tgctcgctgc ggtgctgctc aaccgcctcg tctattacca ggagatggcg gaaaaggcgg
     4801 cgatggagtc cacggcgaga ctgctcaaga ccggcctcca gatccgcctc gccgaactga
     4861 tcatcacgaa ccggcaatcc gaggcggcgg cgctcgaaca ggaaaacccg gtccagtggc
     4921 tggaaacgag gccgtccaat tatggagggc tgattcacga cgattccagg cccggcgcct
     4981 ggtatttcga cgagcgggag cggcagttgg tgtacgtcgt gaataccggc aatcgtctgg
     5041 atctcggcgg cgaaactggg ccgaagcagg tccgttacag cgctcgcctg ctgagggaca
     5101 gggtgcgaac ggccggcggc gtggtggaaa gcgtgacggg ggtcacgtta gtgccggttt
     5161 tgccctaccg ctggccttga ggtatccacc ggcgtatact ggcacgcgtt ttgctgctgt
     5221 atcaatggga tgcacgcgat ttcttgtgag aaatgtcacg ttatgacggc gtcgtcgagc
     5281 gcaaagggtt ttaccctgat cgagctgatc gtggtgatca tcatcctgag catattggcc
     5341 gcgaccgcat tgccgcgatt cgccaacctg caggtgcagg cacgcgttgc caagctgaac
     5401 ggcgcgatgg ggtcgatgaa gggcgcatcg tctttggcgc acgcaggttg tctcgcgtcc
     5461 acgccgcagt gcacgacgcc gccgtcgaac accggtactc tggatatgga aggggtgccc
     5521 gtcacgatga tccatgctta tcccacggcc gattcggcgg gaatcgttac tgccgcggga
     5581 atcagcgccg ggccggcctc gacggatggc tacgacgtgc agggtggcgg ttcttccgcc
     5641 ggcagcgtcc tgcggctgat ggtggatggc aacgacccgg tcaactgcag ctttacctac
     5701 acggcgccca acggtcccaa ccagtcgccg gtgttcagcg cgctggtggt aaccggctgc
     5761 tgaacggcag cgagcacgac ttcattacat tccagtcatt caatcgcatt atctgtttgc
     5821 agttcgaaag gagagcacca tgaagacgca acagggtttt acgctggtcg agctgatcgt
     5881 ggtcatcgtg atactcggca tcctcgccgc gaccgcgctg ccgcgtttca tcaacgtgac
     5941 gaccgacgcg cgcatcgctg cggtggaagg catcgcggga ggcttgagag gcgcagtagg
     6001 cctggcgcag tccaagtatt tcgccgttgg cagttcggcg atgacgaccg tcgatatgaa
     6061 tggcacctcc gtaacggtag ccgcggttag cggaattccg acgggcgacg ccgccggcat
     6121 tggcaccgct gcccaactcg ggggcgtgac agccaactat gcggtgacac ccaatacgtt
     6181 ccggccgagt ggcggcagcg cgacttgtga agcgcgctat gccccgtcca ccgg
//
LOCUS       DBNGSG010000137         4282 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_386830, whole genome shotgun sequence.
ACCESSION   DBNGSG010000137 DBNGSG010000000
VERSION     DBNGSG010000137.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4282)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_386830"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="AC158Q_03535"
     CDS             <1..453
                     /locus_tag="AC158Q_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HIR3338871.1"
                     /translation="AIYFSPQHPVMAELAKQYHGVLGMMLHGLTTLPFWFAAAGLATA
                     WYLYIARPGLPAVLRRKAGVLVTILMEKYGFDRFNDWFFAGGARLVGGGLWKGGDVAV
                     IDGFVNGSARAVGWFAVVIRALQSGYIYHYAFAMIIGILVLLTMMFLA"
     gene            464..1948
                     /locus_tag="AC158Q_03540"
     CDS             464..1948
                     /locus_tag="AC158Q_03540"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HIR3338872.1"
                     /translation="MTGLPLLSLAIWIPILAGVLVLATGSDRNAPVARLIALAGAVLG
                     FAVTLPLYTGYDPNASGFQFEQRLPWIESFNVNYHLGIDGISLLLILLNSFTTVLVVI
                     AGWEIIKERVAQYLAAFLFLSGFMNGVFAALDALLFYVFFEATLIPMFIIIGVWGGPN
                     RVYAAVKFFLYTLAGSLLTLVALIYLYNVSEGSFSLLDYYRLPLAIEPQILIFLAFMA
                     AFAVKVPMWPVHTWLPDAHVEAPTGGSVVLAAIMLKLGAYGFIRLSLPILPDASRELA
                     WFVIALSLIAVVYIGLVALVQADMKKLIAYSSISHMGFVTLGIFIFNAQGLEGAVIQM
                     ISHGFISGALFLCVGVLYDRVHSRQIADYGGVVNTMPVFAALFMLFAMANSGLPGTSG
                     FVGEFMVIMGAVKVNFWYAFAAATTLVFGAAYTLWMYKRVVFGAVANHHVADLKDANG
                     RELLVLGLLAVAVLAMGIWPYPFAEVLHVPVNDLLNHVAQSKLR"
     gene            1959..3413
                     /gene="nuoN"
                     /locus_tag="AC158Q_03545"
     CDS             1959..3413
                     /gene="nuoN"
                     /locus_tag="AC158Q_03545"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008115450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HIR3338873.1"
                     /translation="MHNIMPLLPAYPELFLLTMACAILVVDLFVSDDNRVLTYALTQA
                     ALAGAFAITLLTAVAEPVTTFSGMFVDDLMSDVLKLLTYLAVMTMLVYSRAYLAARGL
                     FRGEYFVLVLFATLGMMVMISANHLLTLYLGLELLSLALYSMVALQRDSATATEAAMK
                     YFVLGALASGMLLYGMSMLYGATGALGLAQLSDAIVSGAGGTRNFTLVFALVFIVAGL
                     GFKLGAVPFHMWVPDVYHGAPTAVTLFIGSAPKLAAFAFIMRLLVQGLGADWLVGEWQ
                     QMLVVMAVLSLAIGNVTAIAQTNLKRMLAYSTIGHMGFLLLGILSGEIIGYGAGMFYV
                     VVYVLMNLGAFGMILLLSRAGFESENLEDFKGLNQRNPWYAFLMLLLMFSMAGVPPTV
                     GFYAKLSVLQAVINAGYVWLAVVAVLFSLVGAYYYLRLVRLMYFDVPADTAPIAPRLD
                     VSIVMAVNGMAMLVFGIFPGWLMGLCLSAIEKSL"
     gene            3508..4089
                     /locus_tag="AC158Q_03550"
     CDS             3508..4089
                     /locus_tag="AC158Q_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR3338874.1"
                     /translation="MAKKLSDFTETRVSSKTVYRGKLLHVLEDEVRLPNGRSARREYI
                     RHPGAAVMVPMPDPQTVVLVRQFRYPLARHFYEMPAGKIDPGEDPLATAKRELLEECG
                     YEAAEWGHMATIHPCIGYADERMEFYLARRLRDVGHRPDEDEFLEKVTVRFAQALRWV
                     REGEITDQKTVIGLLWADRFCSERRKAKGEKRK"
BASE COUNT          635 a         1343 c         1397 g          907 t
ORIGIN      
        1 gcgatctatt tctcgccgca gcacccggtg atggcggagc tcgcaaagca gtatcacgga
       61 gtcctgggga tgatgctgca tggactgacc acgctcccgt tctggttcgc ggccgccggc
      121 ctcgcgacgg cctggtacct ctatatcgcg cggcccggtc tgccggcagt tctgcggcgc
      181 aaggccggcg tgctggtgac gatactgatg gagaagtacg gattcgaccg cttcaacgac
      241 tggtttttcg cgggcggcgc gcgtctcgtc ggcgggggcc tgtggaaggg cggggacgtg
      301 gcggtgatcg acggtttcgt caacggctcg gcacgcgcgg tcggctggtt tgcggtcgtg
      361 atccgcgccc tgcagtccgg ctacatctac cactacgcct tcgccatgat catcggcatc
      421 ctcgtcctgc tgacgatgat gttccttgcc tgagcccgcc atgctgactg gcctgccgct
      481 gctctccctc gccatctgga ttccgattct agccggggtc ctggtgctgg ccacgggctc
      541 cgaccgcaat gccccggttg cgcgcctgat cgcgctcgcc ggcgccgtgc tcggttttgc
      601 cgtcacgctg ccgctctaca cgggctatga cccgaacgcg agcggcttcc agttcgagca
      661 gcggttgccg tggatcgaat ccttcaacgt caactaccac ctcggcattg acggcatttc
      721 gctgctgctg atcctcctga acagtttcac caccgtgctg gtggtaatcg ccggctggga
      781 gatcatcaag gaacgcgtcg cgcagtacct cgcggcgttc ctgttcctct cgggcttcat
      841 gaacggcgtg ttcgccgcgc tggacgcgct tctgttctac gtgttcttcg aggcgacact
      901 gatccctatg ttcatcatca tcggcgtgtg gggcgggccg aaccgggttt atgccgcggt
      961 caagttcttc ctctatacgc tggcgggctc gctgctcacg ctggtcgcgc tgatctatct
     1021 ctacaacgta tcggagggca gtttctcgct gctcgattat taccggctgc cgcttgccat
     1081 cgagccccag atcctgatct tcctcgcatt catggcggcg ttcgccgtga aagtgccgat
     1141 gtggccggta cacacctggc tgccggacgc gcacgtcgag gcgccgaccg gcggctcggt
     1201 ggtgctggcc gcgatcatgc tgaagctcgg cgcctacggc ttcatccgcc tgtcgctgcc
     1261 gattctgccc gatgcgtcgc gcgagcttgc ctggtttgtc attgcattgt ccctgattgc
     1321 cgtggtctac atcgggctgg tggcgctggt gcaggccgac atgaagaagc tgatcgccta
     1381 ttcctcgatc tcccacatgg gcttcgttac gctcggcatc ttcatcttca atgctcaagg
     1441 gctggaaggc gcggtcatcc agatgatctc gcacggtttc atctcgggcg cgctgttcct
     1501 gtgcgtcggc gtgctctacg accgggtgca ctcgcggcaa atcgccgatt acggcggggt
     1561 ggtcaacacc atgccggtgt tcgcggcgct gttcatgctg ttcgcgatgg cgaattccgg
     1621 cctgcccggc accagcggct tcgtcggcga gttcatggtg atcatgggcg cggtcaaggt
     1681 caatttctgg tacgcgttcg cggccgccac cacgctcgtc ttcggcgcgg cctacacgct
     1741 gtggatgtac aagcgcgtgg ttttcggggc ggtcgccaac caccacgtcg cggacctgaa
     1801 ggacgccaac ggccgcgagc tgttggtact gggactgctc gccgtggcgg tgctggcgat
     1861 gggcatctgg ccctatccgt ttgccgaggt gctgcatgtc ccggtcaacg acctgttgaa
     1921 ccatgtggcg cagagcaagc tgcggtaggg ggacagccat gcacaacatc atgccgctcc
     1981 tgccagccta tccggaactg ttcctgctca cgatggcgtg cgcgatcctg gtcgtggacc
     2041 tcttcgtgag cgacgacaac cgcgtcctca cctacgcgct cacgcaggcg gcgctcgccg
     2101 gtgcttttgc gatcacgctt ctcaccgcgg tcgccgagcc ggtcaccaca ttcagcggca
     2161 tgttcgtgga cgacctcatg tcggacgtgc tcaagctgct cacctacctc gcggtgatga
     2221 cgatgctggt ctattcgcgc gcctatctcg cggcgcgcgg cctgttccgc ggcgagtatt
     2281 tcgtgctggt gctgtttgcc acgctgggca tgatggtcat gatctcggcg aatcatctgc
     2341 tcacgctcta cctggggctg gagctgctct cgctagcgct ctactcgatg gtggcgctgc
     2401 agcgcgattc ggcgacggcg accgaggcgg cgatgaagta cttcgtgctg ggggcgctcg
     2461 cctcgggcat gctgctctac ggcatgtcca tgctctacgg ggcgaccggc gcgcttggcc
     2521 tcgcgcagct gtcggacgcc atcgtctccg gcgcaggcgg gacccgcaac ttcacgctgg
     2581 tgttcgcgct ggtgttcatc gtcgccggcc tgggcttcaa gctgggcgcg gtgccgttcc
     2641 acatgtgggt gccggacgtc tatcatggcg cgccgacggc ggtgacgctg ttcatcggct
     2701 cggctcccaa gctcgccgcg tttgcgttca tcatgcgcct gctggtgcag ggtctcggcg
     2761 cggactggct ggtcggggaa tggcagcaaa tgctcgtcgt gatggcggtg ctatcgcttg
     2821 cgatcggcaa cgtcaccgcg atagcccaga ccaacttgaa gcgcatgctg gcctattcca
     2881 cgatcgggca catgggattc ctcctgctcg gcatcctgtc cggggaaatc atcggatacg
     2941 gcgccggcat gttctacgtg gtggtttacg tgctgatgaa ccttggcgcc ttcggcatga
     3001 ttctgctgct ctcccgcgcg ggcttcgaat cggagaacct ggaggacttc aagggtctga
     3061 accagcgcaa cccgtggtac gccttcctca tgctgctcct gatgttctcg atggcgggcg
     3121 tgccgccgac ggtgggcttt tacgcgaagc tgtcggtgct gcaggcggtc atcaacgccg
     3181 gttacgtgtg gctggccgtg gtggcggtgc tgttctcgct ggtcggggcg tactactacc
     3241 tgcgcctggt caggctcatg tacttcgatg tgccggctga cacggcgccg atcgccccgc
     3301 gcctcgacgt gagcatcgta atggccgtaa acggcatggc catgctggtc ttcggcatat
     3361 tcccgggctg gctgatgggg ctgtgcctgt ccgcaatcga aaaatcactt tagcagcggc
     3421 aggcagggca tcggcaagcg gcagctcgcc gctgccggct ttacatgcaa tctgcagccg
     3481 ctcaccgctt actggagcca gtcggaaatg gccaaaaagc tatcggattt caccgaaaca
     3541 cgcgtttcct cgaagaccgt gtaccgcggc aagctgctgc acgtgctgga ggacgaggtg
     3601 cgtctgccca acggccgcag cgcccggcgc gagtacatcc ggcatccggg ggcggcggtg
     3661 atggtgccga tgccggaccc gcagaccgtg gtgctcgtgc gccagtttcg ctacccgctc
     3721 gcccggcatt tctacgagat gccggccggc aaaatcgacc ccggcgagga cccgctcgcc
     3781 acggcaaagc gggagctgct ggaggaatgc ggctacgagg ccgcggagtg gggccatatg
     3841 gccactatcc atccctgcat cggttatgcc gacgagcgga tggagttcta cctcgcgcgg
     3901 cgcctcaggg acgtcggcca caggccggac gaagatgagt tcctggaaaa ggtgaccgtc
     3961 cggtttgcgc aagccctgcg ctgggtgagg gaaggggaaa tcaccgacca gaagaccgtc
     4021 atcggtctgc tgtgggccga caggttttgt tcggaaaggc gaaaggcgaa aggcgaaaag
     4081 cgaaagtaag aagaggccgt caggcgggtg ttattccgac ttccgccttc gcagttcagg
     4141 ccgcgctggc ggcgcggttg tcgagggccg gcttggcggc gtgctcgagc agggtcggcc
     4201 gctggaacag gtagccctgg ccgaactggg tgcccatgcc aaggagcagg gtctgcacct
     4261 ccggggtttc cacccattcc gc
//
LOCUS       DBNGSG010000138         3179 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4123048, whole genome shotgun sequence.
ACCESSION   DBNGSG010000138 DBNGSG010000000
VERSION     DBNGSG010000138.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4123048"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="AC158Q_03555"
     CDS             complement(<1..616)
                     /locus_tag="AC158Q_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697214.1"
                     /note="catalyzes the formation of
                     4-amino-2-methyl-5-phosphomethylpyrimidine from
                     5-amino-1-(5-phospho-D-ribosyl)imidazole and
                     S-adenosyl-L-methionine in thiamine biosynthesis; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HIR3338875.1"
                     /translation="MNANPKFLNATAHVDDAAVKPLPNSRKVYVEGSRPDIRVPMREI
                     TQSDTPIHSGAAAPAGAPARSEPNPPIFVYDTSGPYTDPSVNIDIRSGLAPLRQKWIE
                     ERNDTEALDGPSSRYGRERLADPKLAQLRFNLKRNPRRAKPGMNVSQMHYARRGIITP
                     EMEYIAIRENQRLDALPALITRQHKGESFGAASFPTSAKAITPEF"
     regulatory      complement(682..783)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(825..1268)
                     /locus_tag="AC158Q_03560"
     CDS             complement(825..1268)
                     /locus_tag="AC158Q_03560"
                     /inference="COORDINATES: protein motif:HMM:NF024970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3338876.1"
                     /translation="MTLPQPRRFEASLEFFQELRGHAERYCADAGIDREVSGRLVLVL
                     EELFANTVEHGYRQVAGGAAEKPVWLTLTAAGNAVEVIYEDAAPAHDPFSKAVAPDYS
                     GPADRWQVGGVGVALVIKLGRNVSYDRVRDRNCIRFTIPTRRAGT"
     gene            1364..1819
                     /locus_tag="AC158Q_03565"
     CDS             1364..1819
                     /locus_tag="AC158Q_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HIR3338877.1"
                     /translation="MKLSILRSFVTFVAAAMLATSALANDVGQIKSAKGAVHIERDGQ
                     KLPAAVGAKVRAADTVVTGADGSVGITFVDNSLLSAGPNSTLAIERYTFDPTTHAGAF
                     DTRLARGTLSVVSGKIARQTPGAMTVRTPAAILGVRSTEFVVRAEELPR"
     gene            1821..2420
                     /locus_tag="AC158Q_03570"
     CDS             1821..2420
                     /locus_tag="AC158Q_03570"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3338878.1"
                     /translation="MRLIALLPVLALAAGCASPKPAPRNDMVVVLPERDGKVGAVVVR
                     EGSRETVLDSAYASARSSEASGMRAAPSSAEEVRKVFGPALSAQPARPVSLTLYFTEG
                     STLSAESKAVVDRMFAEIAQRPAAEIVVIGHTDTIGADAYNDKLSLERAEKMRELLIG
                     LGVPSQNISAAGRGKRELLVPTADNVNEPRNRRVEISVR"
     gene            complement(2460..>3179)
                     /locus_tag="AC158Q_03575"
     CDS             complement(2460..>3179)
                     /locus_tag="AC158Q_03575"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR3338879.1"
                     /translation="IQTGILPDARDAFPGETRFSVYAALEPAREVGGDLYDFFMLDGD
                     RLFFLIGDVSGKGLPASMFMAMSKALCKSIAARHPDSVSGTLRDASRDIGRENPEQFF
                     VTLLAGVLDARSGALRLANAGHDAPYLMRSGSVERLAADGGPPLCTVEDFPYRESTFT
                     LAPGDTLCMVTDGITEAMNARGELYGTARLAALIGRLAREPAGTAPERIGAAIREDVA
                     RYCGGAEPADDLTILAVRWTG"
BASE COUNT          507 a         1104 c         1070 g          498 t
ORIGIN      
        1 cgaattccgg cgtgatcgcc ttcgcgctgg ttggaaatga ggcggccccg aacgactcgc
       61 ccttgtgctg ccgggtaatg agcgccggca gcgcatccag gcgctggttc tcgcggatgg
      121 cgatgtactc catctcaggc gtgatgatgc cgcggcgcgc gtagtgcatt tgcgacacgt
      181 tcatccccgg tttcgcccgg cgcgggttgc gcttgaggtt gaagcgcagt tgcgcgagtt
      241 tcgggtcggc gagacgctcg cgcccgtaac gcgagctggg gccgtcgagc gcctcggtat
      301 cgttgcgctc ctcgatccac ttctggcgca agggcgccag acccgaacgg atgtcgatgt
      361 tcaccgacgg gtcggtgtac gggcccgagg tgtcgtagac aaagatcggc gggttgggct
      421 cggaacgcgc cggggccccg gccggcgcgg ccgcaccgga atgaatcggc gtgtccgact
      481 gcgtgatctc gcgcatcggc acgcggatgt ccgggcgcga gccctcgacg tagaccttgc
      541 gcgagttggg cagcggcttc accgccgcat catcgacgtg cgcggtggcg ttgagaaact
      601 tcggattggc gttcatgtgc gctccttgcg gcaaacggct cgagacaggc tcgagacaaa
      661 aattcggggg cgcggggaag ggaaaacgga ttcaacgctt ccctgcgccg gcattatccg
      721 gatcaggttc gaagggactc tctcacccga agaccgctgt cttcaggacc cctacgttgg
      781 tctccaaacg ttactccaag gcctgcaggg gcgcaagagc gcaatcaggt tccggcgcgc
      841 cgggtaggga tggtgaagcg gatgcagtta cggtcccgga cgcgatcgta gctcacgttg
      901 cgcccgagct tgatgaccag ggccaccccc accccgccga cctgccagcg gtcggccggc
      961 ccggaatagt cgggcgcgac ggccttggaa aacgggtcgt gcgccggcgc cgcgtcctcg
     1021 taaatcacct cgacggcatt gccggcggcc gtcagggtca gccagacggg cttttcggcc
     1081 gcgccccccg cgacctggcg atagccatgc tccacggtat tggcgaacag ctcctcgagc
     1141 acgaggacca gccggccgct gacctcgcgg tcgatgcccg catcggcgca gtaacgctcg
     1201 gcatggccgc gcagctcctg aaagaactcc aggctggcct cgaagcggcg gggctgcggc
     1261 agggtcatac ggggcacccg gagcggaaat gcgtcacggc cggtttcatg gaaactcggt
     1321 tattataagt agcattcacc gtcagccgcc ccggggagac aacatgaagc tttccatcct
     1381 tcgttccttc gtcactttcg ttgcggccgc gatgctggca acgagcgcac tggccaacga
     1441 cgtcggccag atcaagtccg cgaagggcgc ggtccacatc gagcgcgacg ggcagaaact
     1501 gccggccgcg gtcggcgcca aggtgcgcgc tgccgacacc gtggtgaccg gcgctgacgg
     1561 ctcggtcggc attactttcg tcgacaactc gctgctctcg gccggcccga acagcacgct
     1621 cgcgatcgag cgctacacgt tcgacccgac cacccatgcc ggcgccttcg acacgcggct
     1681 tgcgcgcggc acactctccg tggtgtcggg caagatcgcc cggcagacac ccggcgcgat
     1741 gacggtcagg acccccgccg ccatcctggg cgtgcgcagc accgagttcg tggtgcgcgc
     1801 cgaggaattg ccccgctgag atgcgtctca tcgcgctatt gcccgtgctc gcattggcgg
     1861 ccggctgcgc ctcgcccaag ccggcgccgc gcaacgacat ggtcgtcgtg ctgccggagc
     1921 gggatggaaa ggtcggcgcg gtcgtcgtca gggagggctc gcgcgagacc gtcctcgaca
     1981 gcgcctacgc ctcggcccgc tccagcgagg ccagcgggat gcgcgctgcg ccctccagcg
     2041 ccgaggaagt gcgcaaggtg ttcggacccg cgctctccgc gcagccggcg cgcccggtct
     2101 ccctgacgct ctacttcacc gaaggcagca cgctgtccgc cgagtcgaag gcggtcgtgg
     2161 accggatgtt cgcggagatc gcgcagcggc ccgcggccga gatcgtcgtg atcgggcaca
     2221 ccgacaccat cggcgccgac gcctacaacg acaagctctc cctcgagcgc gccgagaaga
     2281 tgcgcgaatt gctgatcggg ctcggcgtgc cgtcgcagaa catcagcgcc gccggccgcg
     2341 gcaagcgcga gctgctggtg ccgaccgccg acaacgtcaa cgagccccgc aaccggcgcg
     2401 tcgagatcag cgtgcgctag cgtttcgctc gtctctcgtc tctcgtcact cgtcactctt
     2461 caccccgtcc agcggacggc cagaatggtg aggtcgtccg ccggctccgc gcccccgcag
     2521 tagcgcgcca cgtcctccct gattgccgca ccgatgcgct ccggcgccgt gcctgccggc
     2581 tcgcgcgcaa gccgcccgat gagcgcggcg agccgcgcgg tgccgtacag ttcgccgcgc
     2641 gcgttcatcg cctcggtgat gccgtcggtc accatgcaca gcgtgtcgcc cggcgcgagc
     2701 gtgaaagtgc tctcccggta gggaaaatcc tcgaccgtgc acaagggcgg cccgccgtcc
     2761 gccgcaaggc gctccacgct cccgctgcgc atgaggtagg gagcgtcgtg gcccgcgttg
     2821 gcgagccgca gcgcgccgct gcgcgcatcg agcaccccgg ccagcagcgt gacgaagaac
     2881 tgctccggat tctcgcgccc gatatcgcgc gacgcatcgc gcagcgtccc ggacacgctg
     2941 tccgggtggc gcgcggcgat gctcttgcac agcgccttgc tcatcgccat gaacatgctg
     3001 gccggcagcc ccttgcccga tacgtcgccg atgaggaaga agagccggtc cccgtccagc
     3061 atgaagaaat cgtaaagatc gccgccgacc tcgcgcgccg gctcgagcgc ggcgtaaacc
     3121 gagaagcgcg tctcgcccgg aaacgcatcg cgggcgtcgg gcaggatgcc cgtctgaat
//
LOCUS       DBNGSG010000139        10978 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2086817, whole genome shotgun sequence.
ACCESSION   DBNGSG010000139 DBNGSG010000000
VERSION     DBNGSG010000139.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 10978)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10978)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10978
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2086817"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1535
                     /locus_tag="AC158Q_03580"
     CDS             <1..1535
                     /locus_tag="AC158Q_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HIR3338880.1"
                     /translation="DIRTHGIRNSHLLSIAPTGTISLAFADNASNGIEPAYSWTYQRK
                     KRMPDGSMKTYDVEDHAWRLYRHLGGDMEKLPPAFVTAMDISALDHMRMVAAVAPYVD
                     SAISKTVNVPEDYPYEPFKDLYLEAWKAGLKGITTYRPNKVLGSVLSLAPSPEAEQPN
                     DLDTSADRRIRLEAAPQPALSSLRWPGRPRLTAGNPSWTYMVENPAGPFAIFVGHVEN
                     ADPHAFEVWVNGAEQPRGLGAVAKTLSMDMRAQDKAWLRMKLEILARTAGDDAFDCPM
                     PPDGEVQRVPSLVAAFARIVRYRVDQLGALARADGASPVLDALYAKKEPKTGPSGTMS
                     WTADVHNARAGDDFVLILKELQLPGGQRRPYSVWMAGEYPRALDGLCKLLSLDMRVID
                     PAWIGMKLRKLLNYAEPLGDFMARVPGENRQQNYPSTLAYCARLIIHRYAMLGVLDER
                     GYPLSEMGVLEIPEDDRVTARAVPYRLLHGKRCGECGNAAVIHKDGCEFCTACGAIGA
                     CG"
     gene            1829..2452
                     /gene="pdxH"
                     /locus_tag="AC158Q_03585"
     CDS             1829..2452
                     /gene="pdxH"
                     /locus_tag="AC158Q_03585"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012399931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HIR3338881.1"
                     /translation="MPRVADIRKPLSESGVETDPIAQFARWYERALDAVKPLPDAVAL
                     ASATRAGRPSLRMVLLKGFDAHGFVFYTNYRSRKGRELARNARASLLFYWGGLERQVR
                     VDGRVEKVSARESDAYFATRPRGSQLSAWASAQSRTVAGRAALERRFAAYAHEYSGAV
                     PRPPHWGGYRLAPDAIEFWQGREDRLHDRILYRRGPRGRWIIERLAP"
     gene            2452..3681
                     /locus_tag="AC158Q_03590"
     CDS             2452..3681
                     /locus_tag="AC158Q_03590"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR3338882.1"
                     /translation="MRVFIRALALPAVPLALLAAAFALVYYSDALPPSLAGLKVYGPY
                     IVFALGATLSLAFNRGRALLALAVLASAYGAQQAWLQQGLDAPTARAVYAGLTVFVPV
                     NLAILAVLPELGTFNRHGTLRLGVIVLQAVFVFWVATTGQAGIIDWVYQKFLDPAPFS
                     AGRIPQLGLALIGVALLVTMTAALIMRSATHASLAGAIVAFAVAAHVPNASLTFSIFT
                     MTAELMVTVAVLQDTFRMAFRDELTGLPSRRALNERLAALGNRYAIAMLDVDHFKKFN
                     DTYGHDLGDQVLKMVAAHMADVGGGGKAYRYGGEEFTVLFPGKEASEALPYLERLREE
                     IDSYRLARRGVERPKKAQGNKRQRGSWRDKDSVSVTISIGVAERNDRLVTPQAVLEAA
                     DRALYRAKEKGRNRVSR"
     gene            3682..4602
                     /locus_tag="AC158Q_03595"
     CDS             3682..4602
                     /locus_tag="AC158Q_03595"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3338883.1"
                     /translation="MAPPPARHAGFDAFLAWYFVTVWGSGFIATKIGLQYAPPFTFLT
                     LRFLFGLACLVPILLMTQPRFPGTRTELGHVVVAGLLMHAIHLGGSHYTQYLGMSAGI
                     TALILSVQPLITALVAVRWMNERLQLRQWAGVAIGLAGVALIVWHKVDVREATVGSLV
                     AVTVALAGVTAGTLYQRVYCPLVDLRSATFIQFVVTVAALAPLAWLVEGFAVTWAWPM
                     VGSIAFLVIGASMLAVSALHTLMRHGQATRVTSLVYLTPIFAVVLELATFGVMPSALS
                     LAGIAVTCGGVALVAWRSSLSARPANASTL"
     gene            4654..5784
                     /locus_tag="AC158Q_03600"
     CDS             4654..5784
                     /locus_tag="AC158Q_03600"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3338884.1"
                     /translation="MSIRLLVFLTVLTHLSFAGFRVVLSLMAIHHEASPFIVGVVMAL
                     LTVVALLFAVRWGRWVDRVGVRGPMLAGVAAIFAAMALVWAAPRLETLFLASPLAGSG
                     FFLFHIAAGQAAAMIGRPEDRVKNFSLLALGFSASGFLGPMLAGFAIDGIGHANAMLA
                     LSAGALVNGAVLAARKVEVRRHAPAAVHEGKRRISDLLRIPGFRLVFIVSGTLSMTWD
                     LFAFAIPLYGSQLGLSASTIGIILGAFGAAVFAVRLALPLIAHRLDEWRMLIAAMLLS
                     GATLLVFPLVNGVPLLLALSFLLGIGLGGTQPFLMALLYEKAPEGRGAEVLSVRTWII
                     NFCQTSVPLASGALGAALGMLPVFWAMGASLLVAGWFAARGK"
     gene            complement(5912..6211)
                     /locus_tag="AC158Q_03605"
     CDS             complement(5912..6211)
                     /locus_tag="AC158Q_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HIR3338885.1"
                     /translation="MAKAKKSKPKKAKKSGRKPNAAFMKPMSTSSTLAAVIGSGSMPR
                     TEVTKKLWAYIKRNGLQDKTNRRMINADDKLRAVFGGKSQVSMFDMTKLVSKHLS"
     gene            complement(6631..7173)
                     /gene="msrA"
                     /locus_tag="AC158Q_03610"
     CDS             complement(6631..7173)
                     /gene="msrA"
                     /locus_tag="AC158Q_03610"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 - L-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HIR3338886.1"
                     /translation="MPAGREIATLAGGCFWCLEAVYDGMKGVESVESGYMGGRSPNPT
                     YEQVCGGNTGHAEVVRVTFDPAAVSFKELLEVFFVIHDPTTLNRQGNDSGTQYRSAIF
                     YHSPQQKSDAEEVIGRLNAEKLWSGPIVTELAPVSAFYQAEGYHQEYYARNPYQPYCQ
                     FVVAPKVAKFRKHFLDKLKK"
     gene            complement(7289..8119)
                     /locus_tag="AC158Q_03615"
     CDS             complement(7289..8119)
                     /locus_tag="AC158Q_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338887.1"
                     /translation="MQAVATITTNQREQSEQARVRALVRFNGLTFHSIAMASFLETAV
                     PLQVNRLAQVFGAHPDASLWLEQIWWPQRAELGRQLREYIEATWPEFDWSAAYQEFCE
                     PYRPRSGLEKRDSGAALEALALCVTAAQAAMFYRALANSADEPALRALARNAACGHAG
                     HFDYFRALFERCKRSGRVGFAATWRTVIAVSRCAREHDVASAFQALGQNWSRAPVVPA
                     LGFPEYRERMARLIQRHAAPGTVERMLFRPWLARERAAPVLQTPAARRDRWLPAAQPA
                     "
     gene            8295..9059
                     /locus_tag="AC158Q_03620"
     CDS             8295..9059
                     /locus_tag="AC158Q_03620"
                     /inference="COORDINATES: protein motif:HMM:NF024835.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3338888.1"
                     /translation="MAHSGALVEVLKRELRSRGVTYAQVARKLGLSEASVKRMFSRRE
                     FTLKRLDQVCQLTHSDFSDLARTLNREESLISQLSPEQEKEIASSIKLFLVAVCVLNH
                     VPPEQIEEIYEVSQAECIQLLVKLDRIGFIRLLPGNRIRLLVSRDFAWLPDGPIQRFF
                     KQHAHGDFVRSRFDRPDEYFLAVNGMLSGSSRATMVARLKRIAREFSELHNDDVRLPP
                     SQRLNMTMLVAIRHWELQAFTELRRKKPTPTSGNRT"
     gene            9056..9694
                     /gene="msrA"
                     /locus_tag="AC158Q_03625"
     CDS             9056..9694
                     /gene="msrA"
                     /locus_tag="AC158Q_03625"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 - L-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HIR3338889.1"
                     /translation="MNTRRSLLPLLWAAALAIGAVSFVPAHAQPNPGAKPVTARATFA
                     GGCFWCMEEVYDKVPGVIATTSGYMGGSVKNPSYELVSTGRTGHTEVVQVDYDPAKVS
                     YARLVDVFWRNIDPTQKDAQFCDYGSQYRSGIFFHDEEQKRIAEASRTALQKSKPFKG
                     EIVTEITKASEFYAAEGYHQGYYQKNPARYKFYKTGCGREARLQQLWGKPGS"
     gene            9691..10353
                     /locus_tag="AC158Q_03630"
     CDS             9691..10353
                     /locus_tag="AC158Q_03630"
                     /inference="COORDINATES: protein motif:HMM:NF016424.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HIR3338890.1"
                     /translation="MTGALLDAQADFELKQRVAALESATGVEAVVSVIARADSYPEIP
                     WKAFALGASLASLAAVAAALLEPGWEAAGAVAETAVAALAAGGAAALATVWVAPFARL
                     FLPRTRRRAEVLQYAQAMFLEAELHRTRRRDGILLLVSLFEHEVVVLADRGVREKLGS
                     AGLGPVVAATTARLARGLLKDALLEGLARLEQVLEENGFRAQPGDANELPDSVIQRRE
                     PS"
     gene            10353..>10978
                     /locus_tag="AC158Q_03635"
     CDS             10353..>10978
                     /locus_tag="AC158Q_03635"
                     /inference="COORDINATES: protein motif:HMM:NF016424.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HIR3338891.1"
                     /translation="MRAPRWLLVALLAFPLAGEAQRSDIPFLTGRVVDNAEIVKPDTR
                     RRITGQLQAHEQKTTNQIAVLTVPTLGGESVEEYSTRVFEQWKLGQKGKDNGVLVVVV
                     PQDRRMRIEVGYGLEGTLPDAIAARIIRNVMTPRFKEGDYDGGIAQGVGAIIAQLEGK
                     PSQADDESPQRARDTEFIKAPDMALHEKVLFGAFIFGIIGLFTFIGIVT"
BASE COUNT         1756 a         3647 c         3609 g         1966 t
ORIGIN      
        1 gcgatatccg cacgcacggc atccgcaaca gccacctgct ctcgatcgcg cccaccggca
       61 cgatctcgct cgcgttcgcc gacaacgcct ccaacggcat cgagcccgcg tactcgtgga
      121 cctaccagcg caagaagcgc atgcccgacg gctcgatgaa gacctacgac gtcgaggacc
      181 acgcgtggcg gctctaccgc cacctcggcg gcgacatgga gaagctcccg cccgccttcg
      241 tcacggcgat ggacatctcc gcgctcgacc acatgcgcat ggtcgcggcg gtcgcccctt
      301 acgtcgattc cgcgatcagc aagacggtaa acgtgcccga ggactacccc tacgagccat
      361 tcaaggacct ctatctggaa gcctggaagg ccgggttgaa aggcatcacg acgtatcgcc
      421 ccaacaaggt tctgggcagc gtgctctcgc tcgccccctc gcccgaagcc gagcagccga
      481 acgacctcga caccagcgcg gaccgccgca tccgcctgga agcggcgcca cagcccgcgc
      541 tctcgagcct gcgctggccc gggcgccccc ggctcaccgc cggcaacccg tcgtggacct
      601 atatggtcga aaatccggcc ggcccgttcg ccatcttcgt cggccacgtg gagaacgccg
      661 acccgcacgc attcgaagta tgggtgaacg gcgccgagca gccgcgcggg ctgggggcgg
      721 tggccaagac gctctcgatg gacatgcgcg cgcaggacaa ggcctggctg cggatgaagc
      781 tggagattct cgcgcgcacc gccggcgacg atgccttcga ctgcccgatg ccgcccgacg
      841 gcgaagtcca gcgcgtgccc agcctggtgg cggcattcgc gcgcatcgtg cgctaccgcg
      901 tggaccagct cggcgcgctg gcgagggcgg acggcgcctc gccggtgctc gatgcgctgt
      961 acgcgaaaaa ggagcccaag accgggcctt ccggcacgat gtcgtggacg gcggacgtgc
     1021 acaatgcgcg cgccggcgat gatttcgtgc tgatcctgaa ggagctgcag ctgcccggcg
     1081 gacagcgccg gccgtactcg gtgtggatgg cgggggagta cccgcgcgcg ctcgacgggc
     1141 tgtgcaagct gctctcgctg gacatgcgcg tgatcgatcc cgcgtggatc ggcatgaagc
     1201 tgaggaaact cctcaactac gccgagccgc tcggcgactt catggcgcgc gtgccggggg
     1261 agaatcgcca gcagaactac cccagcaccc tcgcctactg cgcccggctc atcatccacc
     1321 gctacgcgat gctgggcgtg ctcgacgagc gcggttatcc cctctccgag atgggcgtgc
     1381 tcgaaatccc ggaggatgac cgggtcacgg cgcgcgcggt cccgtaccga ctcctgcacg
     1441 ggaaaaggtg cggagaatgc ggcaacgcgg ccgtgatcca caaggacggg tgcgagttct
     1501 gcaccgcgtg cggcgcgatc ggcgcgtgcg gctgaacggc aagagctgaa ccacaaggac
     1561 acgaaggacg caaaggaaaa accaagtcga aattgaaccg ccaagcacgc caagagtaac
     1621 aaaagcatag ccatagagaa cgccgttttt tgaagttatc cgcgtgtatc taggtttatc
     1681 tgcggttcca ttaattgttt ttcttggcgt gcttggcggc cttggcggtt cgattattgt
     1741 tcttgtttct cttggcgtct tggcggttca atcgttgttt ttgtttttcc tggcggttca
     1801 gcgaccaatt tcaccgtaac gcggtaaaat tccgcgcgtg gcggatatcc gtaagccatt
     1861 gagcgagtca ggcgtcgaaa ccgatcccat cgcgcagttc gcgcgatggt acgagcgcgc
     1921 gctcgatgcc gtgaaaccgc tgccggacgc ggtggcgttg gcgagcgcga cccgcgccgg
     1981 ccgcccttcg ctgcgcatgg tgctgctgaa gggctttgac gcccacggtt tcgtgttcta
     2041 caccaactac cgcagccgca aggggcgcga gctggcgcgc aacgcgcgcg cgagcctgct
     2101 gttctactgg ggcgggctcg agcgccaggt gcgggtggac ggccgcgtgg aaaaagtgtc
     2161 cgcgcgcgag tcggacgcct atttcgccac gcgcccgcgc ggcagccagc tctccgcctg
     2221 ggcctccgcg caaagcagga ccgtggccgg ccgtgccgcg ctcgaacgcc gtttcgccgc
     2281 ttacgcgcat gaatattccg gcgccgtgcc gcgcccgccg cactggggcg gttaccgcct
     2341 cgcgcccgac gcaatcgagt tctggcaggg ccgcgaagac cgcctgcacg accgcattct
     2401 ttaccgccgc ggcccgcgcg ggcgctggat catcgagcgc ctcgcaccct gatgcgcgtc
     2461 ttcatccggg cgctcgccct gcccgccgtt ccgctcgcac tgctggcggc ggcattcgcc
     2521 ctcgtctact acagcgacgc gctgccgccg tcccttgccg ggctcaaggt ttacggcccc
     2581 tatatcgtat tcgcgctcgg cgcaacgcta tcgctggcgt tcaaccgcgg gcgcgcgctg
     2641 ctggcgctgg ccgttctggc gagcgcgtac ggcgcgcagc aagcgtggct gcagcagggg
     2701 ctcgacgccc cgacggcgcg ggcggtctac gccgggctga cggtgttcgt gccggtcaac
     2761 ctcgcgatcc tcgccgtgct gccggaactc ggcacattca accgccacgg cactttgcgg
     2821 ctcggcgtga tcgtccttca ggccgttttt gtgttctggg tcgcaacgac gggccaggcg
     2881 ggcatcatcg actgggtcta ccagaaattt ctcgatccgg cgccgttcag cgcgggccgt
     2941 atcccgcagc ttggcctcgc cctgatcgga gttgccttgc tggtcacgat gacggcggcg
     3001 ctgataatgc gttccgccac ccatgcaagc ctggcgggcg cgattgtcgc gttcgcggtc
     3061 gcggcccacg tgcccaatgc ctcactcact ttctcgatct tcaccatgac ggcggaactg
     3121 atggtgaccg ttgccgtgct gcaggacact ttccgcatgg cgttccgcga cgagctcacc
     3181 gggctgccga gccgccgggc gctgaacgag cggctggctg cgctcggcaa ccgctacgcc
     3241 atcgcgatgc tcgacgtgga ccacttcaag aaattcaacg acacctacgg ccacgatctt
     3301 ggcgaccagg tgctgaaaat ggtggccgcg cacatggccg acgtgggcgg cggcggaaag
     3361 gcctaccgct acggcggcga ggagttcacc gttctgttcc ccggcaagga agccagtgag
     3421 gcattgccgt acctggagcg gttgcgcgag gaaatcgatt cgtaccgcct cgcgcggcgc
     3481 ggcgtggagc ggccgaagaa ggcgcagggc aacaagcgcc agcgtggcag ctggcgcgac
     3541 aaggacagcg tgtcggtcac gatcagcatc ggcgtggcgg aacgcaacga ccgcctcgtc
     3601 accccgcagg cggtgctcga agccgccgac cgcgcgctct accgcgccaa ggaaaagggc
     3661 cgcaaccggg tgagccggta gatggccccg ccgccggctc ggcacgcggg attcgacgct
     3721 tttctggcct ggtacttcgt gacggtctgg ggctcgggct tcatagccac caagatcggg
     3781 ctgcagtacg cgcccccctt cactttcctg acgctgcgtt ttttgttcgg gctggcgtgc
     3841 ctggtgccga tcctgctcat gacacagccg cgctttcccg gcacgcgcac cgaactcggg
     3901 cacgtcgtcg tcgcgggatt gctgatgcac gccatccatc tcggcggcag tcactacacg
     3961 caatatctcg gcatgtcggc cggcatcacc gcgctgatcc tgtcggtgca gccgctgatc
     4021 accgcgctgg tcgcggtgcg ctggatgaac gagcggcttc aacttcgcca gtgggcgggc
     4081 gtggcaatcg ggcttgcagg cgtcgcgctc atcgtgtggc acaaggttga cgtgcgggaa
     4141 gccaccgtcg gcagtctcgt cgccgtcacc gttgcgctgg caggcgtcac cgccgggacg
     4201 ctctaccagc gcgtttactg cccgctcgtg gacctgcgca gcgcgacgtt catccagttc
     4261 gtcgtgacag tagcggcgct cgcgccgctc gcgtggctgg tcgagggctt cgcggtaacc
     4321 tgggcttggc cgatggtcgg atccatcgcc tttctcgtga tcggcgcctc gatgctcgcg
     4381 gtgagcgctc tgcacaccct gatgcggcac ggccaggcca cccgcgtcac cagtctcgtc
     4441 tatctcacgc cgatcttcgc ggtggtgctg gagctggcga cgttcggggt catgccgagc
     4501 gcgctctcgc tcgccggcat cgccgtcacc tgcggcggcg tggcgctggt cgcatggcgt
     4561 tcctccttat cggcgcgccc cgcgaacgca tccacattgt gaagcggcac ggaacgggcg
     4621 ggtagaattc atgatttctc cgggcgcgcc gacttgagca tccgcctgct cgtttttctt
     4681 accgtcctca cgcacctgtc gttcgccggt ttccgcgtgg tgctctcgct gatggcgatc
     4741 caccacgaag cctctccctt catcgtcggc gtggtgatgg cgctgctgac cgtggtcgcc
     4801 ctgctgttcg cggtgcgctg ggggcgctgg gtggaccgcg tcggcgtgcg cgggccgatg
     4861 ctcgccggcg tggcggccat cttcgcggcc atggcgctgg tatgggcggc gccgcgcctg
     4921 gagacgctgt ttcttgcgag cccgcttgcc ggctcggggt tttttctctt tcacatcgcg
     4981 gccggccagg cagcggccat gatcggccgg cccgaggacc gcgtgaagaa tttcagcctg
     5041 ctcgcgcttg gcttctcggc ctcgggcttt ctcgggccga tgctcgccgg cttcgctatc
     5101 gacggcatcg gccacgccaa cgcgatgctg gcgctctcgg ccggcgccct ggtgaacggg
     5161 gcggtgctgg cggcgcgcaa ggtcgaagtg cggcgtcacg cgccggctgc cgtccacgaa
     5221 ggaaaacggc gaatctccga cctgttgcgc atccccggct tccggctggt attcatcgtg
     5281 agcggcacgc tgtccatgac ctgggatctg ttcgccttcg ccatcccgct ttacggctcg
     5341 cagctcgggc tttccgcgtc cactatcggc atcatcctcg gcgcgttcgg cgcggcggtg
     5401 ttcgccgtca ggttggcgct gccgctcatc gcgcaccgcc tggacgagtg gcgcatgctg
     5461 atcgcggcga tgctgctctc cggcgcgacg ctgctcgttt ttccgctggt caatggcgtg
     5521 ccgctgctgc tggcgctgtc cttcctgctg ggcatcgggc tcggcggaac gcaaccgttc
     5581 ctgatggcgc tgctctacga gaaagcgccg gagggacgcg gcgccgaagt gctgagcgtg
     5641 cgcacctgga tcatcaattt ctgccagacc agcgtgccgc tggcctccgg cgccctcggc
     5701 gccgcgctcg gcatgttgcc ggtattctgg gcgatgggcg cgtcgctgct cgtcgccggc
     5761 tggtttgccg cgcgcggcaa ataatgccgt gagcagtgag cggtcccggc gtgttctcgt
     5821 gtggggtgtc ctgctgaccg ctgactcgcg ctgaacagcg caaaaaaagg ccgcgtctcc
     5881 gcggcccttt tttatgcgac gtgaagcgcg actagctgag gtgcttgctg accagcttgg
     5941 tcatatcgaa catcgacacc tgcgacttgc cgccgaacac cgcacgcagc ttgtcgtcgg
     6001 cattgatcat gcgccggttg gtcttgtcct gcaggccgtt gcgcttgatg taggcccaca
     6061 gcttcttggt gacctcggtg cgcggcatcg agccgctgcc gatcaccgcc gcgagcgtgg
     6121 agctcgtgct catcggtttc atgaacgcgg cattcggctt gcggccgctc ttcttcgcct
     6181 tcttgggttt gcttttcttt gctttcgcca tcgactatct cccgttgatt gcgctgaacc
     6241 agcgcgggtg gttggtaacg actctgaccg tgaattcggc gagtagagga tgccaatgtc
     6301 cagccaacct tgcaaggggt ttttgctgtt tttcgctctg aaacgagcgc ttcgccgccc
     6361 gtttgcgagc agcgcgagcg cctgctgtgg atacttttca cgtggcccgc cgcgcgcgtt
     6421 tcagcagcgc cctcgcagca cggcgccgcc ctgccacggc atgtagtcaa ggacttatcc
     6481 gcacaagttc aagtctcgat gtgcaagtcc attgccacga aatcacgacg atacgctgcg
     6541 cgatttcgct cgccttgcgg tggcaggcgc ggggatgcgg tgggaaaccg tcggcccggg
     6601 cacccccccg tcgggggcgg gcgacgcccg ttacttcttg agcttgtcca ggaaatgctt
     6661 tcggaacttg gcgactttcg gggcgaccac gaactggcag taaggctggt acgggttgcg
     6721 cgcgtagtac tcctggtggt aaccctccgc ctggtagaag gcggacaccg gcgccaactc
     6781 ggtgacgatg gggccgctcc acagtttctc ggcgttgaga cgcccgatca cttcctcggc
     6841 gtccgacttc tgctgcggcg agtgatagaa aatcgccgag cggtactggg tgcccgagtc
     6901 gttgccctgg cggttcaatg tggtcggatc gtggatcacg aagaacactt ccagcagctc
     6961 tttgaacgat accgccgcgg gatcgaaagt gacgcgcacg acctcggcat gcccggtgtt
     7021 gccgccgcac acctgctcgt aggtcgggtt gggcgagcgc ccgcccatgt agcccgattc
     7081 gacggactcg acgcctttca tgccgtcgta gaccgcctcg agacaccaga aacaaccgcc
     7141 ggcgagcgtg gcgatttccc ggccggcagg cattgtggtg gcggaattca atgcaggact
     7201 ccggtgaatg atccggacct ctgatacgaa acgccgagtg tagcggattc acggcgcaga
     7261 cggaggaccg cccgcgccgc ctgctccctc acgccggttg cgccgccggc aaccaccggt
     7321 cccgcctggc ggccggcgtc tgcagcaccg gggccgcgcg ttcgcgcgca agccatgggc
     7381 ggaacagcat gcgctcgacc gtccccggcg cggcatgccg ctggatcagc cgggccatgc
     7441 gttcccggta ctccggaaaa ccgagggccg gcacaaccgg cgcgcggctc cagttctggc
     7501 ccagcgcctg gaacgcgctg gccacgtcat gctcgcgcgc gcaacggctg accgcgatca
     7561 ccgtgcgcca cgtggcggcg aacccgacgc gcccgcttcg cttgcagcgc tcgaacagcg
     7621 cgcgaaaata atcgaaatgg cccgcatggc cgcacgctgc gttgcgcgcc agtgcgcgca
     7681 aggccggctc gtccgcgctg ttggccaggg cgcggtagaa catcgcggcc tgggccgccg
     7741 tgacgcagag cgccagcgcc tcgagcgcgg cgccgctgtc ccgcttttcc agcccggagc
     7801 gcggacgata aggctcgcag aattcctgat aagcggcgct ccagtcgaac tcgggccagg
     7861 ttgcctcgat gtactcccgc agctgccggc cgagttcggc ccgctgcggc caccagatct
     7921 gctcgagcca taagctcgcg tcgggatgag cgccgaaaac ctgcgcgagg cggttgacct
     7981 gcagcggcac ggccgtctcc aggaaggagg ccatggcaat actgtggaac gtcaatccgt
     8041 tgaagcggac gagcgcgcgc acccgggcct gctcgctctg ctcgcgttga ttcgtcgtga
     8101 tggttgcgac cgcttgcatg gttccctcct ttatgtgagt cgcgggcggt tgcctgcgtg
     8161 cgaaaccagt attgctgcgg tgcaataagc cgggcaagca aaggttacag cgtcgctacc
     8221 cgctctcctg acggtattat taggcgctac cagggggggt tgttacggcg ggagtcacaa
     8281 cggggaggcc gtccatggcg cacagcggtg cattggtgga agtgcttaaa cgcgaattga
     8341 gatcgcgcgg cgtcacctac gcccaggtcg cgcgcaagct gggactaagc gaagcgagcg
     8401 tcaaacgcat gttttcccgc agggaattca cgctcaaacg gctggaccag gtctgccagc
     8461 tgacgcattc cgacttctcc gacctggcgc gcacgctcaa ccgggaggaa tccctgatct
     8521 cccagttgag cccggagcag gagaaggaga tcgcctccag catcaagctg tttctggtcg
     8581 cggtgtgcgt gctgaaccac gtccctcccg agcagataga ggaaatttac gaggtttccc
     8641 aggccgagtg catccagctg ctggtcaagc tcgaccgcat cgggttcatc cggttgctgc
     8701 ccggtaatcg tataaggctt ctggtatccc gcgatttcgc gtggcttccc gacgggccga
     8761 tccagcgctt cttcaagcag cacgcgcacg gcgatttcgt gcgctcgcgc ttcgaccgcc
     8821 ctgacgagta cttcctggcg gtgaacggca tgctgtcggg cagctcccgc gccacgatgg
     8881 tggcgcggct gaaacggata gcgcgggaat tctcggagtt gcacaatgac gacgtccggc
     8941 tgcccccgtc gcagcgcttg aacatgacca tgctggtcgc gatccgccac tgggagctcc
     9001 aggctttcac cgagttgaga cgcaagaaac cgacacccac atcaggaaac cgaacatgaa
     9061 tacgagaaga tctttattgc cgctgctctg ggccgccgcc ctcgccatcg gtgccgtcag
     9121 cttcgtgccg gcacacgcac agcccaatcc gggcgccaag cctgtcacgg ccagggcgac
     9181 gttcgccggc ggctgtttct ggtgcatgga agaggtgtac gacaaggtgc ccggggtgat
     9241 cgccaccacc tccggctaca tgggcgggag cgtcaagaac ccgagctacg aactggtgag
     9301 caccggccgc accgggcata ccgaggtggt gcaggtggat tacgatcccg ccaaggtgag
     9361 ctacgcgagg ctggtggacg tattctggcg caatatcgac ccgacccaga aggacgcgca
     9421 gttctgcgac tacggctcgc agtaccgcag cggcatcttc tttcacgacg aggaacagaa
     9481 gcggatcgcc gaggcgtccc ggaccgcgct gcagaaaagc aagccgttca agggcgaaat
     9541 cgtcaccgaa atcaccaagg catccgagtt ctacgccgcc gaaggctacc accagggtta
     9601 ctaccagaag aatcccgctc gctacaagtt ctacaagacc ggctgcgggc gcgaagcgcg
     9661 cctgcagcag ctttggggca agccaggctc gtgaccggcg ctcttctcga cgcgcaggcg
     9721 gacttcgaac tcaagcagcg ggtggccgct ctcgaaagcg cgaccggcgt cgaagcggtc
     9781 gtctcggtca tcgctcgcgc tgattcctat cccgagattc cctggaaggc atttgcgctg
     9841 ggcgcctccc tcgcgtcgct cgccgccgtc gccgcggcgc tgttggagcc gggctgggag
     9901 gctgccgggg cggtcgcgga aaccgccgtg gcggcgctgg ccgccggcgg agcagcggct
     9961 ctcgccaccg tgtgggtcgc gccgttcgca cgcctgtttc tgccgcgcac ccggcgccgg
    10021 gcggaagtcc tgcagtatgc gcaggcgatg ttcctcgagg ccgaactgca ccgcacgcgc
    10081 cgccgcgacg gaatcctcct gctggtgagc ctgttcgagc atgaagttgt agtgctcgcc
    10141 gaccgcgggg tacgggaaaa gctgggatcg gcgggtctcg ggcctgtcgt tgccgcaacg
    10201 accgcccggc tggcgcgcgg cctgctgaag gacgccctcc tcgagggcct ggcgcggctg
    10261 gaacaggtgc tggaagaaaa cggttttcgc gcgcagccgg gggacgccaa cgaactgccc
    10321 gactcggtga tacagcggcg ggagccctcg tgatgcgggc cccgcgctgg ctgctggtgg
    10381 ccttgctggc attcccgctt gcgggcgagg cccagcgctc cgatatcccg ttcctcaccg
    10441 gccgcgtggt ggacaacgcc gagatcgtga agcccgacac ccggcgccgg atcaccgggc
    10501 agttgcaggc gcacgagcag aagaccacca accagatcgc ggtcctgacg gtaccgacac
    10561 tcggcggcga aagtgtcgag gagtactcca cgcgcgtgtt cgagcagtgg aaactgggac
    10621 agaaaggcaa ggacaacggc gtcctggtgg tggtcgtgcc gcaggaccgc cgcatgcgca
    10681 tcgaggtcgg ttacggcctg gaaggaacgc tgccggacgc catcgcggcc cgcatcatcc
    10741 gcaacgtgat gacgccgcgc ttcaaggagg gtgactacga cggcggcatc gcccagggcg
    10801 tcggcgcgat catcgctcaa ctggaaggca agccgtcgca ggcagacgat gaaagcccgc
    10861 aacgcgcgcg ggacacggag ttcatcaagg cccccgacat ggcgctgcac gagaaggtgc
    10921 tgttcggcgc tttcattttc ggcatcatcg ggctattcac attcatcggc atcgtcac
//
LOCUS       DBNGSG010000140         6008 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2766284, whole genome shotgun sequence.
ACCESSION   DBNGSG010000140 DBNGSG010000000
VERSION     DBNGSG010000140.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6008)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6008)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6008
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2766284"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..1609)
                     /locus_tag="AC158Q_03640"
     CDS             complement(68..1609)
                     /locus_tag="AC158Q_03640"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HIR3338892.1"
                     /translation="MAKEKLIIFDTTMRDGEQSPGAAMTKEEKLRIARQLERMRVDVI
                     EAGFPASSDGDFDAVQAVARAIKDSTVCGLARANEKDVRRCGDAVRVAKSPRLHTFIA
                     TSPIHMEKKLRMEPSQVIEQAVRAVKWAREYTDNIEFSPEDAGRSDIDFLCRILEQVI
                     KAGATTINVPDTVGYTLPDQFGRLIRQLRERIPNSDKAVWSVHCHNDLGLAVANSLSA
                     VLNGARQVECTVNGLGERAGNASLEEIVMAVRTRQDVFPCDTGIDATQIVPASKLISG
                     ITGYPVQPNKAIVGANAFAHESGIHQDGVLKHRETYEIMSAESVGWSTNRLTLGKLSG
                     RNAFKTRLQEIGIEIGTEEALNAAFKRFKDLADKKREIFDEDLQALITEEEIEHIENE
                     RYRLVSLTAHSETGEQPFAKIVVSENGIERPSEARGSGPVDASFKAIEAVAKSGAELL
                     LYSVNNITSGTDSQGEVTVRLTKGGRIVNGQGADTDIVVASAKAYINALNKLHSNLER
                     LNPQV"
     gene            complement(1872..2705)
                     /gene="pssA"
                     /locus_tag="AC158Q_03645"
     CDS             complement(1872..2705)
                     /gene="pssA"
                     /locus_tag="AC158Q_03645"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HIR3338893.1"
                     /translation="MSMYEPREHKLFIRSRFARRGIYWLPNLFTIAALFAGFYAIVQA
                     MEGRFEQAAIAIFVAMVLDGLDGRIARLTRTQSAFGAELDSLSDMVSFGVAPALVVYV
                     WALKDFAGVTTVALLGPWLSTKLGWIAAFIYCACAALRLARFNTTLDIADKRFFQGLP
                     SPAAACVVAGLVWALNEYQIKGADVKWFAWGLTVFAGLTMVSNCKFYSGKDINLRKSV
                     PFSVVVGITFGMVALFMLSSTLPEMLFLLFMAYGCSGYVIWIYDAFKARAGDRTSPPA
                     A"
     gene            complement(2796..3461)
                     /locus_tag="AC158Q_03650"
     CDS             complement(2796..3461)
                     /locus_tag="AC158Q_03650"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003261915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HIR3338894.1"
                     /translation="MAGALAGVSAYPHPIIAKEGWPFIAAVTAAAAGVHWFAGAGWAA
                     PLWLIAAFMVQFFRDPPRVIPQALKAVLSPADGRIVAVERTRDPYLERDALKMSVFMN
                     VFNVHSNRSPVDGEICDAWYHRGSFFNAALSKSSLENERAALWIRTPGGADVTCVQIA
                     GLIARRILCYVSPGRWLARGERYGFIRFGSRVDVYLPPESRPVASMGDKVYATESILA
                     TLP"
     gene            complement(3499..4515)
                     /gene="ilvC"
                     /locus_tag="AC158Q_03655"
     CDS             complement(3499..4515)
                     /gene="ilvC"
                     /locus_tag="AC158Q_03655"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HIR3338895.1"
                     /translation="MKIYYDKDADLSLIKRKHVAIIGYGSQGHAHANNLKDSGVRVTV
                     GLRKGGASWNKAKNAGIAVKSVGDAVKSADIVMILLPDEHHAQVYHDEIEPNIKKGAT
                     LAFAHGFNIHFKQIEPRADLDVIMIAPKGPGHLVRSTYTQGGGVPSLIAVAQNPSKKA
                     RDIALSYAAAIGAARAGVIETTFREECETDLFGEQVVLCGGLTALIQAGYDTLIEAGY
                     APEMAYFECLHEVKLIVDLIYEGGIANMRYSVSNTAEYGDFTRGPRIINEQTRAEMKK
                     ILQEIQTGQFAKEFILENRAGAPTLKAMRRIGQAHSVETVGAKLRDMMPWIRKNRLVD
                     QSKN"
     gene            complement(4614..5105)
                     /gene="ilvN"
                     /locus_tag="AC158Q_03660"
     CDS             complement(4614..5105)
                     /gene="ilvN"
                     /locus_tag="AC158Q_03660"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HIR3338896.1"
                     /translation="MRHIISVLLENESGALSRVAGLFSARGYNIESLTVAPTEDSTLS
                     RMTIVTAGSDEVIEQITKQLNKLVDVVKLVDLSDGDHIERELMLVKVRAVGKDREEMK
                     RMVDIFRGRIIDVTDKDYTIELTGTGAKLDAFLKAIDRGAILETVRTGASGIGRGDRV
                     LRV"
     gene            complement(5102..>6008)
                     /locus_tag="AC158Q_03665"
     CDS             complement(5102..>6008)
                     /locus_tag="AC158Q_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR3338897.1"
                     /translation="MSMQHCDVLLAIGARFDDRVIGNPEHFYNAQRTIIHVDIDPSSI
                     SKRVKVDIPIVGNVREVLRELIKQLEASRDKPDQDALRDWWAQIGEWRSRDCLKYDRS
                     SSLIKPQYVIEKLYEITRGDAFVTSDVGQHQMWAAQFYKFDKPRRWINSGGLGTMGFG
                     LPAAMGVQLAHPGSPVACVTGEASIQMCIQELSTCRQYRLPIKVVNLNNKYMGMVRQW
                     QEFFHGNRYAESYMDALPDFVKLAEAYGHVGVKVEKPADVEPALKEAFSRKDDLVFMD
                     FITDQTENVFPMVPGGKGLSEMILV"
BASE COUNT         1056 a         1960 c         1843 g         1149 t
ORIGIN      
        1 ctcctcttat cacatcggcg gctgacaagc ccgcgcgccg ccgtcgtcaa ccgcgattcc
       61 cggccggtca aacctgcggg ttcagccgct cgaggttcga gtgcagcttg ttgagcgcgt
      121 tgatataggc cttcgccgaa gccacgacga tatcggtgtc agcgccctgg ccgttgacga
      181 tgcgcccgcc tttggtcagc cgcacggtca cttcaccctg cgaatcggtg ccgctcgtga
      241 tgttgttgac cgagtagagc agtagttcgg ccccgctttt cgcgaccgcc tcgatcgcct
      301 tgaacgaggc gtccaccggg ccgctgcccc tggcttcgct cgggcgctcg ataccgttct
      361 cggacacgac aatcttcgcg aacggctgct cgccggtctc ggaatgggca gtcaaagaca
      421 ccagccggta gcgctcgttc tcgatgtgct cgatctcctc ttcggtgatc aaggcctgca
      481 agtcctcgtc gaagatctcg cgtttcttgt cggcgaggtc cttgaagcgc ttgaaggcgg
      541 cgttcagcgc ctcctcggtc cctatttcga tgccgatttc ctgcaaccgc gtcttgaacg
      601 cgttgcgacc cgagagcttg ccgagcgtca ggcgattggt gctccagccc actgattcgg
      661 cgctcatgat ttcgtaggtc tcgcggtgct tcaacacccc gtcctggtgg atgccggatt
      721 catgtgcaaa tgcgttcgcc ccgacgatcg ccttgttggg ctgcaccggg tagccggtga
      781 tgccggaaat caacttcgat gccggcacga tctgcgtagc gtcgatgccg gtatcgcacg
      841 gaaacacgtc ctggcgcgtt cgcaccgcca ttacgatctc ctcgagcgag gcgttgccgg
      901 cgcgctcgcc caggccgttc accgtgcact cgacctggcg tgcaccgttc agcacggcgg
      961 aaagcgaatt ggcgaccgca agcccgaggt cgttgtggca atgcaccgac cacacggcct
     1021 tgtcggagtt gggaatgcgc tcgcgcagct gcctgatcag ccggccgaac tggtccggca
     1081 gcgtgtagcc gacggtatca ggaacattga tcgtggtggc gccggccttg attacctgct
     1141 cgaggatgcg gcacagaaaa tcgatgtcgg aacgcccggc atcttccggg gaaaactcga
     1201 tgttgtcggt gtactcgcgc gcccatttca ccgcccgcac cgcctgctcg atcacctgcg
     1261 agggctccat gcgcagcttc ttctccatgt ggatgggcga ggtcgcgatg aaggtgtgca
     1321 ggcgcgggct cttcgcgacg cgcaccgcgt ccccgcagcg ccgcacgtcc ttctcgttgg
     1381 cgcgggcaag tccgcacacc gtgctgtcct tgatggcgcg cgcaaccgcc tgcaccgcat
     1441 cgaaatctcc gtcgctggag gccgggaagc cggcctcgat cacgtccacg cgcatgcgtt
     1501 cgagctggcg cgcgatgcgc agcttctctt ctttcgtcat cgccgcgccc gggctctgct
     1561 ccccgtcacg catcgtggtg tcgaatatga tcagtttttc cttcgccatg gatgtctcca
     1621 ggattgccgc ggcgcccgac cggcctggca gccggagaac gcgcgtgctc tataagtttc
     1681 ccgccgcggc gctcgccgcg cgggccaaaa catcagattg tggggaaagt gtatgtgcgc
     1741 ctaggggcgc agcagcaggc cggccagcag aaggctggca ggaatggaaa gctcgatggc
     1801 ctggatttcg cgatgcatga tgcggaacta taacggtttt cccgcgggga ggcaaactgg
     1861 cggctcccgc cttatgcggc cggtggcgat gtgcgatctc ccgccctcgc cttgaacgca
     1921 tcgtagatcc agatcacata gccggaacaa ccgtacgcca tgaacaggag aaacagcatc
     1981 tccggcagcg tgctggagag catgaacagc gccaccatcc cgaaagtgat gccgactacg
     2041 accgagaatg gcacactttt cctcaggttg atgtcctttc cgctgtagaa cttgcagttg
     2101 ctcaccatgg tgagtccggc gaacacggtc aacccccacg cgaaccactt cacgtccgct
     2161 cccttgatct ggtactcgtt cagcgcccac accaggccgg cgacgacaca cgccgcagcg
     2221 gggctcggca ggccctggaa gaaacgcttg tcggcaatat cgagcgtcgt attgaaacgc
     2281 gcgagcctca acgccgcgca cgcgcaatag atgaaggcgg cgatccagcc gagcttggtg
     2341 gaaagccacg gcccgagcag cgccacggtc gtcacgccgg caaaatcctt gagtgcccac
     2401 acgtacacca ccagggccgg cgccacgccg aacgacacca tgtccgacag gctgtcgagt
     2461 tccgcgccga aggcactctg ggtgcgcgtc aggcgggcga tgcgcccgtc gagaccgtcc
     2521 agcaccatcg ccacgaagat cgcaatggcc gcctgctcga aacggccttc catcgcctgg
     2581 acgatggcat agaagccggc gaacagcgcg gcgatcgtga aaaggttggg cagccagtaa
     2641 atcccgcgcc gggcgaaacg cgaccggatg aacaatttgt gctcgcgcgg ctcgtacatg
     2701 ctcatccccc gtgggtcgtc agccgtgggt cgtgaaaaca gcggaaggga taatgcgatt
     2761 cacgaatcac gattcccgaa tcacgccttt actcgctacg gcagcgtggc gagtatcgat
     2821 tcggttgcgt aaaccttgtc gcccatggaa gcgaccggtc gcgattcggg cggcaggtaa
     2881 acgtccacgc gcgagccgaa gcggatgaag ccgtagcgct caccgcgcgc aagccagcgt
     2941 ccgggactga cataacacag gatgcggcgg gcgatgagtc ctgcgatctg cacgcaggtc
     3001 acatccgccc cgcccggcgt cctgatccac agcgcggcgc gctcgttctc gagagaggac
     3061 ttggacaggg cggcattgaa gaaactgccg cggtggtacc aggcgtcgca aatctcgcca
     3121 tccaccgggc tgcgattgga atggacgttg aacacgttca tgaacacgct catcttgagc
     3181 gcgtcgcgct cgaggtaagg atcgcgcgtg cgctcgactg cgacgatgcg gccatcggcc
     3241 ggcgacagca ccgctttcaa tgcctgcgga atcacgcgcg gcggatcgcg gaagaactgc
     3301 accatgaagg cggcgatcag ccacagcggc gcggcccagc ccgcgccggc gaaccagtgc
     3361 acgcccgctg cggcggccgt caccgccgcg atgaacggcc agccctcctt cgcgataatc
     3421 ggatgcgggt aggcgctcac gccggcaagc gcgccagcca tggtttttaa ctcatcgctc
     3481 atcaattatg gcccatcatc agtttttcga ctggtccacc agccggttct tcctgatcca
     3541 cggcatcatg tcgcgcagct tggcacccac cgtctcgacc gagtgcgcct ggccgatgcg
     3601 gcgcatggcc ttcagtgtgg gcgcgccggc ccggttctcc aggatgaact ctttcgcgaa
     3661 ctgcccggtc tggatctcct gcaggatctt cttcatctct gcgcgcgtct gctcgttgat
     3721 gatacggggg ccgcgcgtga agtcgccgta ctcggccgtg ttcgacaccg agtagcgcat
     3781 gttggcaatg ccgccctcgt agatgagatc cacgatcagc ttgacctcgt ggaggcactc
     3841 gaaataggcc atctccggcg catagccggc ctcgatcagc gtatcgtagc cggcctgtat
     3901 cagcgccgtc aggccgccgc acagcacgac ctgctcgccg aaaagatcgg tctcgcattc
     3961 ttcgcggaac gtggtctcga tcacgcccgc gcgcgcggcg ccgatggccg ccgcatagga
     4021 caacgcgatg tcgcgggcct tcttggacgg gttctgcgcc accgcaatca gcgacggtac
     4081 gccgccgccc tgggtgtagg tcgagcgcac caggtgcccg ggacccttgg gcgcgatcat
     4141 gatgacgtcg aggtcggcgc gcggctcgat ctgcttgaag tgaatgttga aaccgtgggc
     4201 aaacgcgagc gtcgcgccct tcttgatgtt gggctcgatt tcgtcgtgat agacctgcgc
     4261 gtggtgctcg tcgggcagga ggatcatcac gatgtcggcg gacttcacgg cgtcgcccac
     4321 gcttttcacc gcgatgcccg cgttcttcgc cttgttccag gatgcgccgc ccttgcgcag
     4381 gcccacggtc accctgaccc cggaatcctt caggttgttc gcgtgcgcat gtccctgcga
     4441 gccgtagccg atgatggcca cgtgcttgcg cttgatgagc gagagatcgg catccttgtc
     4501 gtaatagatc ttcattgctt tcccttctgt tggatatcga gaggcgatgg acgggggatg
     4561 aaaggcgtca aaccgagcag cgctcccgcc cctcccgtct cccccttccc tgtctagact
     4621 ctgagtaccc ggtcgccgcg gccgatgccg gaggcgccgg tacgcaccgt ttcgagtatc
     4681 gcgccgcggt ctatcgcctt gagaaaagcg tcgagcttgg cgccggtgcc cgtcaactcg
     4741 atggtgtagt ccttgtcggt cacgtcgatg atgcggccgc ggaagatgtc gaccatgcgc
     4801 ttcatctcct cgcgatcctt gcccaccgcg cgcaccttga ccagcatcaa ctcgcgctcg
     4861 atatgatcgc cgtcggacag atcgaccagc ttcaccacgt ccaccagctt gttcagctgc
     4921 ttggtgatct gctcgatcac ctcgtccgaa ccggcggtga cgatggtcat gcgcgagagc
     4981 gtcgagtctt cggtgggcgc gacggtaagc gactcgatgt tgtagccgcg agcggaaaac
     5041 aatccggcta cgcgcgacag cgcacccgat tcgttctcga gcagcacgga gataatgtgc
     5101 ctcataccag gatcatctcc gacaggccct tgccgcccgg caccatcgga aacacgttct
     5161 cggtctggtc cgtgatgaag tccatgaaca cgaggtcgtc cttgcgcgag aacgcctcct
     5221 tcagcgcggg ttccacgtcc gccggcttct cgaccttcac gccgacatgt ccgtaggcct
     5281 ccgccagctt cacgaaatcc ggcagcgcgt ccatgtacga ttcggcatag cggttgccgt
     5341 ggaaaaactc ctgccactgc cgcaccatcc ccatgtactt gttgttgaga ttgaccacct
     5401 tgatgggcag ccggtactgc cggcacgtgg agagttcctg gatgcacatc tggatgctcg
     5461 cctcgccggt gacgcacgcc accggcgacc cggggtgcgc gagctgcacg cccatcgcgg
     5521 cgggaagtcc gaatcccatg gtgccgagcc cgccggaatt gatccagcgc cgcggcttgt
     5581 cgaacttgta gaactgcgcc gcccacatct ggtgctggcc gacgtcggat gtgacgaagg
     5641 catcgccccg ggtgatctcg tagagcttct cgatcacgta ctggggcttg atgagggaac
     5701 tcgagcggtc gtacttgagg cagtcgcgcg agcgccattc cccgatctgc gcccaccagt
     5761 ccctgagcgc gtcctgatcg ggcttgtccc gggaagcctc gagctgcttg atcagctcgc
     5821 gcagcacctc gcgcacgttg ccgacgatgg ggatgtccac tttcacccgc ttggagatgg
     5881 acgacggatc gatgtcgacg tgaatgatgg tgcgctgcgc gttatagaag tgctcgggat
     5941 tgccgatcac gcggtcgtcg aagcgggcac cgatagcgag cagcacgtcg cagtgctgca
     6001 tcgacatg
//
LOCUS       DBNGSG010000141         6110 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1916950, whole genome shotgun sequence.
ACCESSION   DBNGSG010000141 DBNGSG010000000
VERSION     DBNGSG010000141.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6110)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6110)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6110
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1916950"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(310..3174)
                     /locus_tag="AC158Q_03670"
     CDS             complement(310..3174)
                     /locus_tag="AC158Q_03670"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR3338898.1"
                     /translation="MKSLLLPLTFRARLLLGSMLVNVLMLILLIVNGIGVMDDRLNEQ
                     ARIHVEEQKQLLNAALSAPLAAGQQSALEDILARVRRHSGITYLVLFDRDEKLVASAG
                     WARTRPLPAADPPAKKPFAGRSEHIHAVMDIEAAGRRIGKLHFGLSTAFMRAARAELI
                     RENLIVGGLALALSVISMVALSYWLTRNLAQLTRASENLAAGDLNVRLPVEAGGEVGK
                     LTHAFNAMAAALEGRIRALAESEAKFTAIADYSYDCELWINPEGRLIWINPRVLDMFG
                     YTPQECLDTQNFPAPFIADPDVTRTVRQVRRALRGNTGQDYEFRARRKDGTEFWAAAD
                     WRPIYDSRGGHLGIRISIRDITQRKEAEQRLEATVVELRDAQTVQQEYLMRAQDEHAR
                     LSALLAAMDTGILFVSHDNRVVYTNPAFTRIWMIAPGTRLIGGNPQEALASSACVLAR
                     PEDQGKRVLRLPLEDEILGVLEIQMTDGRLITQQVHTVEDVYGRPVGHLWLFQDVTRE
                     RQTADQLIYLAERDALTGLYNRHRFNEELARMIADAQRHNSRVALLFFDLDEFKYIND
                     TFGHRAGDAMLIRVAGEVSGQVRRNELFSRLGGDEFAILVPDISTEMLRVLAERITRA
                     IALVRFQYEGQSLRLTCSLGIAVFPDHADNAEDLIARADTAMYQAKESGKNAWRIYRS
                     DLDSSQQMVRRLSWNDRIMHALENNLMDLQFQGVYNTSDRSLSHFEVLVRMRDKDDPA
                     VLLMPGQFIPVAEKSGKILEIDRWVLRESIQMLAELPSIPAFAVNISGRSFDEPMLPQ
                     YIAEELKRCGVAPRRLMVELTETSAVSDLHDAQRFIEALHQTGCGVSLDDFGTGFSSF
                     AYLKHLQVDSVKIDGLFIRNLPQETDNQLFVKAIVTVARGLHKITIAECVEDEETLVM
                     LKGFGVDCVQGYYLEKPRADHPLLAAASRLQHANLELRFT"
     gene            complement(3212..4540)
                     /gene="argA"
                     /locus_tag="AC158Q_03675"
     CDS             complement(3212..4540)
                     /gene="argA"
                     /locus_tag="AC158Q_03675"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006999805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - L-glutamate N-acetyltransferase
                     activity, acting on acetyl-CoA as donor [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HIR3338899.1"
                     /translation="MRRQRLSAQDRFIDWFQSVAPYIHAFRGKTFVIAFGGDVVADGR
                     FLDLTHDLNLLAALGVRLVLVHGARPQIEAKLKAARRRSRFHKGLRVTDDTALAFAKE
                     ASGRLRVEIEAALSMGLPNSPMAGADIRVASGNFITAKPMGVVEGVDYMHTGEVRKID
                     AGAVQKRLDDGELVLLSPLGYSPTGEVFNLALEDVAASTAAALKAEKLIFLMDTIGVT
                     GRRGELLRNLTVRQAQALLARPETGESDASLYLPCAVRACEHGVKRAHLVSGHIDDGL
                     LLELFTHKGVGTMLTPDPIETLRLAKIEDIGGVLRLIEPLEAEGILVKRGRDRLEREI
                     GRFMVLEHDKLLVGCAALYPFSGERAGELACLVVHPDFRSQGAGERLLTEIEVRARRQ
                     KLKRLFVLTTRAEHWFVERGFDEVGVNQLPRQKRTLYNYQRRSVVLVKRL"
     gene            complement(4728..5462)
                     /locus_tag="AC158Q_03680"
     CDS             complement(4728..5462)
                     /locus_tag="AC158Q_03680"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HIR3338900.1"
                     /translation="MTRIYISGELRPRSELRLPREQAHHVARVLRLAAGAALTLFNGD
                     GAEYDAVVTAVGKSAASVKIGERRPADRESGLEIVLGQALSSGERMDYTVQKAVELGA
                     AAIHPLAASRSVVRLDAERAAKRVAHWQSVAIAACEQCGRNRVPPVTPVATLESWLAQ
                     HGARHAGALRMLLSPLAETRLRDLPAPCGTIVLLAGPEGGFTPEEEARARTGGYTPVR
                     LGPRVLRTETAAVAALAAMQALWGDF"
     gene            5586..>6110
                     /locus_tag="AC158Q_03685"
     CDS             5586..>6110
                     /locus_tag="AC158Q_03685"
                     /inference="COORDINATES: protein motif:HMM:NF014325.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HIR3338901.1"
                     /translation="MKIVILGAGQVGSTVAESLVSEANDITVVDVEPVRLKRLQDRLD
                     LRTVTGNAAHPAVLEQAGARDADMLLAVTQSDETNMVACKLAATMFNIPMKIARIRAA
                     DYLSHPEIFSPENFAVDHSICPEQILCDYIVKLLQFPESLQVLEFADGKVSLVAVRAF
                     HGGPLVGHEIQYLRS"
BASE COUNT          993 a         2079 c         1997 g         1041 t
ORIGIN      
        1 caactcgaaa ctaagagtcc ctaacataat gaaacgcgcg ctgcgctgtc acgattttgg
       61 ctcagggcac gaagcgagtc gcagccaata tgtcgatatt agcaaggcga gcgacaaagc
      121 catgggccaa aactcgtgcc agcccccatg tttcccttgg atattccgat gcctgcgccg
      181 gggttgcggc caaaatcgtc gctgactttg tcggccggct tggccctatc gacatagggc
      241 ctgcgccgtc cttcgcgtca tcaacaattt tggctcgcaa cgcacgcgtg tttcattatg
      301 ttagggactc taagtaaacc gcaattccag attggcgtgc tgcagccgcg aggcggcggc
      361 gagcagggga tggtcggcgc gcggcttctc caggtagtag ccctgcacgc aatccacgcc
      421 aaaacccttc agcatgacga gcgtctcctc gtcctccacg cactcggcga tggtgatctt
      481 gtgcagcccg cgcgccaccg tcacgatcgc cttcacgaac agctggttgt cggtttcctg
      541 cggcaggttg cggatgaaga gcccgtcgat cttcacggag tccacctgca ggtgcttcag
      601 gtaggcgaag gacgagaagc ccgtgccgaa atcgtcgagg gaaacgccgc agccggtctg
      661 gtgcagcgct tcgatgaagc gctgcgcatc gtggagatcg gataccgccg aggtttccgt
      721 gagttccacc atgagccgcc tgggcgccac gccgcagcgc ttcagctcct cggcgatata
      781 ttgcggcagc atcggctcgt cgaacgagcg ccccgagatg ttgaccgcga acgccgggat
      841 cgacggcagt tccgccagca tctggatgga ctcgcgcagc acccagcggt cgatttcgag
      901 gatcttgccc gacttttccg ccaccgggat gaattgcccc ggcatcagca gcaccgcggg
      961 gtcgtccttg tcgcgcatgc gcaccagcac ctcgaaatgc gagagcgagc ggtcggacgt
     1021 gttgtagacc ccctggaact gcaggtccat gaggttgttc tccagcgcgt gcatgatgcg
     1081 gtcgttccag gacagccgcc gcaccatctg ctggctgctg tcgagatcgc tgcggtagat
     1141 gcgccaggcg ttcttgcccg attccttggc ctggtacatc gccgtgtcgg cgcgcgcgat
     1201 caggtcttcc gcgttgtcgg cgtggtcggg gaacacggcg atgccgagcg aacaggtcaa
     1261 ccgcaggctc tggccttcgt actggaagcg caccagcgcg atcgcgcgcg tgatgcgttc
     1321 ggcgagcacg cgcagcatct cggtggaaat atccggcacc aggatcgcga actcgtcgcc
     1381 gcccaggcgc gagaacagtt cgttgcgccg cacctgcccc gatacctcgc ccgcgacgcg
     1441 gatcagcatc gcgtcgcccg cgcggtggcc gaaggtatcg ttgatgtact tgaattcgtc
     1501 gaggtcgaaa aacagcagcg ccacccgcga gttgtggcgc tgggcgtccg cgatcatgcg
     1561 cgcgagctcc tcgttgaagc ggtggcggtt gtagagcccg gtcagcgcgt cgcgctcggc
     1621 gaggtagatc agctggtcgg cggtctggcg ctcgcgggtg acgtcctgga acagccacag
     1681 gtggccgacc ggccggccgt agacgtcctc cacggtgtgc acctgctggg tgatgaggcg
     1741 cccgtccgtc atctggatct cgagcacgcc gagtatctcg tcctcgagcg gcaggcgcag
     1801 cacgcgcttg ccctggtcct ccggccgcgc cagcacgcac gccgagctgg cgagcgcctc
     1861 ctgggggttt ccgccgatca gccgcgtgcc cggggcgatc atccagatgc gggtgaaagc
     1921 gggattggtg tacaccaccc ggttgtcgtg agacacgaac aggatcccgg tgtccatcgc
     1981 ggcgagcagc gccgagagcc gcgcatgctc gtcctgcgcg cgcatcaggt attcctgctg
     2041 gaccgtctgc gcgtcgcgca gctcgaccac cgtcgcttcc agccgctgct ccgcttcctt
     2101 cctctgggtg atgtcgcgga tgctgatgcg gatgccgagg tggccgccgc ggctgtcata
     2161 gatcggccgc cagtcggcag ccgcccagaa ttcggtgccg tccttgcgcc ttgcgcgaaa
     2221 ctcgtagtcc tggccggtat tgccgcgcag cgcgcggcgg acctgccgca cggtgcgcgt
     2281 cacgtccggg tccgcgatga acggtgccgg aaaattctgc gtgtcgaggc actcctgcgg
     2341 cgtgtagccg aacatgtcga gcacgcgcgg gttgatccag atgagtctcc cctccggatt
     2401 gatccacagc tcgcagtcgt agctgtaatc ggcgatcgcg gtgaacttgg cctcgctctc
     2461 ggcaagcgcg cggatgcggc cctcgagcgc agccgccatg gcattgaagg cgtgagtcag
     2521 cttgcccacc tcgccgcccg cttcgaccgg cagccgcacg ttgagatcgc cggccgccag
     2581 gttctcgctt gcccgcgtga gctgcgcgag gttgcgggtc agccagtagg aaagcgcgac
     2641 catcgaaatg acggacaggg ccagcgcgag gccgccgacg atcaggtttt cccggatcag
     2701 ctcggcgcgg gcggcgcgca tgaacgccgt ggacagcccg aaatgcagct tgccgatcct
     2761 gcgtcctgcg gcttcgatgt ccatcacggc gtggatatgc tcgctgcggc cggcgaacgg
     2821 tttcttcgcg ggaggatccg ccgcgggcag cggtctcgtg cgcgcccagc cggcgctcgc
     2881 caccagcttc tcgtcgcggt cgaacagcac gaggtaggta atgccgctgt ggcggcgcac
     2941 gcgcgcgagg atgtcctcga gcgcgctctg ctgccccgcg gcaagcgggg ccgacagcgc
     3001 ggcgttgagc agctgcttct gctcctcgac gtggatgcgc gcctgctcgt tcaggcggtc
     3061 gtccatgacg ccgatgccgt tgacgatcag gagaatcagc atcaacacgt tgaccagcat
     3121 gctgcccagc agcaggcgcg cgcggaatgt cagcggcagc agcagggatt tcacggagag
     3181 gccttcatga ggcgtccact ggaagaagct ttcagaggcg cttcaccagg accaccgagc
     3241 ggcgctggta gttgtacagc gtccgcttct ggcgcggcag ctggttcacg cccacctcgt
     3301 cgaagccgcg ctcgacgaac cagtgctcgg cgcgcgtcgt cagcacgaac aggcgcttca
     3361 gcttctgccg gcgcgcgcgc acttcgatct cggtcagcag ccgctcgccg gcgccctggc
     3421 tgcggaagtc aggatgcacg acgaggcagg cgagttcgcc cgcccgctcg ccggaaaacg
     3481 ggtacagcgc cgcgcagccg accagcagct tgtcgtgctc gagcaccatg aaacgcccga
     3541 tttcccgctc cagccggtcg cggccgcgct tgacgagtat gccttcggct tcgagcggct
     3601 cgatcagcct cagcacgccg ccgatatcct cgatcttcgc cagccgcagc gtctcgatcg
     3661 ggtcgggcgt caacatggtg cccacgccct tgtgggtgaa gagctcgagc agcagcccgt
     3721 cgtcgatgtg ccccgagacg aggtgggcgc gcttcacgcc gtgctcgcac gcgcgcaccg
     3781 cgcatggcag gtacagcgac gcgtcgctct cgcccgtttc aggccgcgcg agcagcgcct
     3841 gggcctggcg cacggtgaga ttgcgcagga gttccccgcg ccgcccggtc accccgatgg
     3901 tgtccatgag aaagatcagc ttctcggcct tcagcgcggc cgcggtggag gcggcgacgt
     3961 cctcgagcgc caggttgaag acttccccgg tgggcgagta ccccaggggc gagagcagca
     4021 ccagctcgcc gtcatcgagg cgcttctgga ccgcgccggc gtcgatcttg cgcacttcgc
     4081 ccgtgtgcat gtaatccacg ccttccacca cgcccatggg cttggccgta atgaaattgc
     4141 cgctcgccac gcggatatcg gcacccgcca tcggcgagtt cggcagaccc atcgagagag
     4201 cggcctcgat ctcgacgcgc agccgcccgc tcgcttcctt ggcgaacgcc agggccgtgt
     4261 cgtcggtcac ccgcagtccc ttgtggaaac ggctgcgccg ccgggccgcc ttcagcttgg
     4321 cctcgatctg ggggcgcgcc ccgtgcacca gcaccagccg cacgccgagg gcggcgagca
     4381 ggttcaggtc gtgcgtgagg tcgaggaagc gcccgtcggc cacgacatcg ccgccgaaag
     4441 cgatgacgaa agtcttgccg cgaaacgcgt ggatataggg cgcgaccgac tgaaaccagt
     4501 cgatgaaccg gtcctgcgcg gacaggcgct ggcgccgcat ggcccgctga acgggctccg
     4561 cgacagcggg ggctgtcgta tttccggcat cggtcttgtg ctcgatggct gtctcccttc
     4621 ccgcatcaca aatttcgtcg ggatatcaag cggatgttat gcgagaaccc ttcccgtgaa
     4681 aagcgtgatt cgtgattgca aagcggtctt ccctcatccc tcatccctca gaagtccccc
     4741 cacagcgcct gcatcgcggc gagcgccgcg accgcggctg tttcggtgcg cagcacgcgc
     4801 gggcccagcc gcactggtgt atagccacct gtccgcgcgc gggcctcctc ttccggcgtg
     4861 aagccgcctt ccggccccgc cagcagcacg atggtgccgc atggcgcggg cagatcgcgc
     4921 agccgggtct cggccagcgg cgacagcagc atgcgcaacg cgccggcgtg acgcgcgccg
     4981 tgctgcgcaa gccatgactc gagcgttgca acgggggtga ccggcggaac gcggttgcgc
     5041 ccgcattgtt cgcacgcggc gatcgccacc gactgccagt gcgcgacgcg ctttgcggcc
     5101 cgctcggcat cgagccgcac cacgctgcgg ctcgcggcca gaggatgaat cgccgccgct
     5161 cccagctcga ccgccttctg cacggtgtaa tccatgcgct ccccgctgga gagcgcctgc
     5221 ccgagcacga tctcgagccc ggactcgcgg tcggcggggc ggcgctctcc gattttcacg
     5281 cttgccgcgc tcttgcccac ggccgtgacc acagcgtcat attcggcgcc gtcgccgttg
     5341 aagagcgtca gcgcagcgcc ggccgccagc cgcagcaccc gcgcgacgtg atgcgcctgc
     5401 tcccgcggca aacgcagctc ggaccgcggc cgcaactccc cggaaatata gatgcgcgtc
     5461 agaaatccgc ctccggttga agtatgccac gcagcgcgcg cgccatcgcc gcggaacgcc
     5521 cgaccggtcc cgtttctccc cgggggcaaa gaggtctaaa atcgcgctct ttcgaaagga
     5581 cgcgcgtgaa gatcgtgatc ctcggggccg ggcaggtcgg ttccacggtt gccgaaagcc
     5641 tggtttccga ggcgaacgac attaccgtgg tcgacgtcga gccggtgcgg ctgaagcgcc
     5701 tgcaggaccg tctcgacctg cggaccgtca ccggcaacgc ggcgcacccg gcggtgctgg
     5761 agcaggccgg ggcgcgcgac gccgacatgc tgctcgccgt gacgcaaagc gacgaaacca
     5821 acatggtcgc ctgcaagctc gccgccacca tgttcaacat cccgatgaag atcgcgcgga
     5881 ttcgtgcagc ggattacctg tcgcatcccg agattttctc gcccgagaac ttcgcggtgg
     5941 accactcgat ctgccccgag cagatcctgt gcgactacat cgtcaagctg ctgcaattcc
     6001 ccgaatcatt gcaggtgctc gagttcgccg acggcaaggt cagcctggtc gccgtgcgcg
     6061 ccttccacgg cgggccgctg gtcgggcacg agatccagta cctgcgcagc
//
LOCUS       DBNGSG010000142        14124 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3615126, whole genome shotgun sequence.
ACCESSION   DBNGSG010000142 DBNGSG010000000
VERSION     DBNGSG010000142.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 14124)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14124)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14124
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3615126"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..1789)
                     /locus_tag="AC158Q_03690"
     CDS             complement(128..1789)
                     /locus_tag="AC158Q_03690"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3338902.1"
                     /translation="MAFVQPSPGLGNQFTADRALRSYLARVLPADVLTEVEPALAAMG
                     ELAGGELYRHQLADRESEPTLRQWDAWGNRVDRIEVTALWHEAERIAAEQGLVATAYE
                     RRHGMYSRVHQFALAYLFTPSNDLYSCPLAMTDGAARALLAAGNQRLIDRAVPHLTSR
                     DPREFWTSGQWMTEAAGGSDVGLTETVARKTGDGWRLYGRKWFTSAIGSQMALTLARP
                     EGNPAGGKGLALFYLETHDGEGRPNGILVNRLKDKLGTRKVPTAELTLEGVPAQLVAG
                     DRDGVRSIAPMLNVTRTWNSVSACAFARRGLALARDYASRRIAFGAPLSEKPLHLDTL
                     AGLEAEFLGALHLTFFVAELMGREEAGKAGEGQEKLLRVLTPVAKLLTGKQAVQILSE
                     VIEAFGGAGYVEDTGLPVLLRDAQVLPIWEGTTNVLSLETLRALEAVGGLAVLQREIG
                     ILLREVREPDLLKISAQVERVMEQAGAWLSGTGGAQLEAGARRFAMTVGRGMELALLV
                     RHAQWSLMNEKDQRARAAARRFAAHGVSLLADMDAGDSRRLARDD"
     gene            complement(1793..2326)
                     /locus_tag="AC158Q_03695"
     CDS             complement(1793..2326)
                     /locus_tag="AC158Q_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338903.1"
                     /translation="MPITPFHFGPGALVKAAVPRHFSFMVFAFSQALIDLEPIGFFLF
                     TGDPVHPYLHTYLGATLIFIASWWAGRPVGEWALRLWNAWLSPAQARWLACEPGISLK
                     AAGTGAFIGAYSHVAIDSIMHGDMEPFAPFSGASPWHLIVSLETLHLACIAAGILGVA
                     GLLALRWLELRQNSRLR"
     gene            complement(2367..3740)
                     /locus_tag="AC158Q_03700"
     CDS             complement(2367..3740)
                     /locus_tag="AC158Q_03700"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3338904.1"
                     /translation="MNAPSAAPSVTEILAEKTAALGPESIPVPVRARAEQLLIDIVGL
                     CVAARGTDYMQALVGAMDGGGPCTAIGHPSGYSAEDAALINGTAVHGEDFDDTFEGGP
                     VHSSAVVIPAVLAACERFGQDGRAALTGIVAGIEATCRLSMVIPKAIHKAGFHPTSVL
                     GTIGGTLAVSRALGLTRRQTVDALGNSASLASGIIEYLAEGAWTKRLHPGWAAKSSIA
                     AARIGRGGFLGPRTALEGVHGLFHGFARSVEGKYGVLTGNFGEEWVMRDITFKPYATG
                     TMNQPYIDCALRLRRHGVQPEEVKEVLCETAEGYVHRLWDPLPSKHRPANAYAAKFSA
                     PFNIAVAFVTGGAGLAAFTEETVRDARILALASKVRYVVDPHNPYPKAYTGHVRMTLK
                     DGRVLEERQPHIRGGAQEPLTRADIETKFRGNCEYGGWSREQADRFLASVPKFFDGPL
                     DLKPLRG"
     gene            complement(3819..4796)
                     /locus_tag="AC158Q_03705"
     CDS             complement(3819..4796)
                     /locus_tag="AC158Q_03705"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3338905.1"
                     /translation="MKFRSVCLLMAAVFPMLAAGPAVAQFKPSKPVEFVTHTGPGGGG
                     DVFARGIAAAMEKEGLMPVRMQVANKTGGGGLTAMAYLAEKKGETHTIAVFTGIWFTI
                     PIMRKEAKFTMKDLTPVVRLVLEPAMIAVKSDSPHKTLKDFIDAAKQKPGELKQSAGS
                     LGSRDWIVRQLLMTHTGASWAYISFPGGGERIAALLGGHVNMMVIEPQEAGEHIRAGN
                     LRVIATVSDKRLPAFPDVPTIQEAGFKIPNVPQVRGIVAPPGIPPEAVAYWEELFARF
                     IKTPSWQKYLADNMFQDGYMRSAELAKFGDEFSESTRKILTAGGVKVAR"
     gene            complement(4829..6340)
                     /locus_tag="AC158Q_03710"
     CDS             complement(4829..6340)
                     /locus_tag="AC158Q_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR3338906.1"
                     /translation="MEVIGHLYQGFAVALTPYNLLWCFIGVFLGTVIGVLPGLGSAAT
                     IAMLLPLTFKMEPTGALIMLAGIYYGSKYGGSTTSILLNVPGESASVVTCLDGYQMAK
                     NGRGGPALGIAAIASFVAGTVGIIGLMLVAPPLAKLALSFSSPEYFALMCLGLAMVVL
                     LAGKSLVKALLAMLVGLWIAGMGTDLFTSESRFTFGSMHMLDGIDFVVVAIGVFALGE
                     VLGNMEIRDGPKMLPVPRGLRNLMPTWAELKACRFAFINGSVVGFLIGVLPGAGSTIA
                     SFISYGIEKAVSKHPEKFGTGVPEGVAAPEGANNSETGGAMVPLLTLGIPGSGTTAIL
                     LAAFILWGLRPGPLMIQDNPTLFWGLVASMYVGNVMLLVLNLPLIPLFAQIMRLPVYV
                     LYPVIFGISMVGVYSVSSSLFDVGLLTVFGLLGYAMRRLDYPAAPLILGLVLGDSMER
                     ALRQSLMMSQGELSILVSRPISATMLFLAVLILLAPMFKKANALRVRALEEGG"
     gene            complement(6421..6876)
                     /locus_tag="AC158Q_03715"
     CDS             complement(6421..6876)
                     /locus_tag="AC158Q_03715"
                     /inference="COORDINATES: protein motif:HMM:NF018979.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HIR3338907.1"
                     /translation="MAKTGDIVSGGALTGLGIFIVVEARGLEYSGLDGPGPGFFPLWY
                     GACMIVLSLLLVGTSLGAAAAGPGGATDWREVGRVLASWSAFALCVALLQVLGFLLAF
                     ALFTFFLIAVMYRRPPGMALAVAVAMSAGFYLLFPLALNVELPIGKLGF"
     gene            complement(6956..7348)
                     /locus_tag="AC158Q_03720"
     CDS             complement(6956..7348)
                     /locus_tag="AC158Q_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3338908.1"
                     /translation="MSRIVHLALKVENLEKATEFYEKVFGFREVETKRTRDHISRHMT
                     DGTLDFTLMKYDEGTRSAESLAAGEKPCIHHFAVEVEDVEASVAGLKQYGCEIVSDPG
                     VIPVKFRAPDGIVAEIVPAKRYKKAKTA"
     gene            complement(7392..8096)
                     /locus_tag="AC158Q_03725"
     CDS             complement(7392..8096)
                     /locus_tag="AC158Q_03725"
                     /inference="COORDINATES: protein motif:HMM:NF024921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR3338909.1"
                     /translation="MALVRILSAGAAQAVIEPIAATWSSETGHEVKADFGAVGAMNAR
                     VVNGEAADVIVLTAALIDELTSAGHVAAGSRADLGKVGTGVAVRAGTPLPDVSNERAL
                     RGNLLAAARLVCPDPATATAGKVVMRALERLGLADTMKSHWQFFPNGYAAMQYLAGSH
                     APYELGITQITEILANPGVTYAGPLPGDLQAMTVYAAGLAARAAEPRAARDFITRLTS
                     AAARPLLARAGYEFDS"
     gene            8282..9082
                     /locus_tag="AC158Q_03730"
     CDS             8282..9082
                     /locus_tag="AC158Q_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3338910.1"
                     /translation="MGVARFFLGCVALLPGVIHAAATESFVNDIEGRPGTFTRVLVVK
                     PEKAVATVILFAGGNGILNIAPDGSVGNSGNFLVRSRESFAQHHLMTAVIDAPSDRLP
                     SPGLSDFRKSAGHAQDIAAVIAFLRKVANLPVWLVGTSRGTTSAANGAVRLQQGGPDG
                     IVLTSSIVKPDDTGTLVTMALEKITVPALVVHHANDACLTTLFQDLPPVLAKLKLAPI
                     EVHQLTIKGGKPPEPGADPCGPFHYHGYLGIEDAVVARISGWIKSGKP"
     gene            9090..9407
                     /locus_tag="AC158Q_03735"
     CDS             9090..9407
                     /locus_tag="AC158Q_03735"
                     /inference="COORDINATES: protein motif:HMM:NF020967.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative signal transducing protein"
                     /protein_id="HIR3338911.1"
                     /translation="MKQIHAAKHAPEAHLVKGFLESNGIAAVVRGEFLTGGWGELPVD
                     VCSVWISDDRQYDRAQALLVAFLRGDFATEFRGQDWRCPKCGEQLEGQFTACWSCGTR
                     RSA"
     gene            9426..10205
                     /locus_tag="AC158Q_03740"
     CDS             9426..10205
                     /locus_tag="AC158Q_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010926057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase/reductase"
                     /protein_id="HIR3338912.1"
                     /translation="MDLELAGLTALVTGASRGIGLAIARQLAREGCNLHLAARTVADL
                     DAARSSILGQGQVAVECHALDLGRSGNAARLAQRCGDVDILINNAGSIPSGHLLEIDE
                     ARWRQSWELKVFGYINLTREVYRTMRARRRGVIVNVIGVAGERLRQDYIAGGTGNAAL
                     MAFTRNLGGESVDYGVRVVGVNPGQIETDRLRQRLERKAKEELGDPGRWRELVLNPPF
                     GRLARAEEVADTVVYLASARASYISGTIVTVDGGRAVRNAT"
     gene            complement(10273..11364)
                     /locus_tag="AC158Q_03745"
     CDS             complement(10273..11364)
                     /locus_tag="AC158Q_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HIR3338913.1"
                     /translation="MIFQHDSIPRLFQPIRFRSVTARNRIMVSPMCQYSAIDGVANDW
                     HFQNLASRAVGGAGFVFVEMTNVEPRGRITAGCMGLWNDAQRDALERIARFVKSQGAV
                     AGIQLAHAGRKASTMRPWEGGRGLAPDEGGWTIIAPSAVPFGEGFPVPQEMDEKTIAG
                     TVALFAAAAGRAREAGFEVIELHGAHGYLISSFLSPISNRRTDRYGGSFDNRTRFLLE
                     VVDAVRSEWPEDKPLFVRISCTDWMEGGWDIEASVKLAQLLKAGGKVDLIDCSSGGVD
                     PRQKVKAFPGYQVPFSAAIRSRAGIATGAVGMISSPDMAEQILAGGEADIIIMARALL
                     NDPYWPIHAAKVLKAKAPWPPQYLRGDIF"
     gene            11451..12227
                     /locus_tag="AC158Q_03750"
     CDS             11451..12227
                     /locus_tag="AC158Q_03750"
                     /EC_number="4.1.3.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014752100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citryl-CoA lyase"
                     /protein_id="HIR3338914.1"
                     /translation="MRKPIRTDIAWSTPSRIIVKGYDLCRDVLGTLSLGDMAFLELTD
                     RLPTARESVVFNAIAVTLVEHGLTPSAIAARMTYTGAPEAMQAAVAAGLCGLGSVFVG
                     SMETAARMLQEAMPDPANEADFDKLAREIVERFHGRKQPVPGIGHPLHKPEDPRAPRL
                     FQIAAENGFDGPYIKLMKQVAQEAGSVYGKSLPINATGAIAAIASELKLPWKIVRGIG
                     VMARAIGLVGHIREEMRAPMAREIKAWAEEEATAHLRTKA"
     gene            complement(12318..13520)
                     /locus_tag="AC158Q_03755"
     CDS             complement(12318..13520)
                     /locus_tag="AC158Q_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR3338915.1"
                     /translation="MRPLDGITVVSLEQAVAAPFATRHLADLGARVIKIERPKVGDFA
                     RDYDRTVKGMASHFVWLNRSKESLTLDVKHSKAKDILERLIARADVFVQNLAPGAASR
                     LGLEAEGLLERHPRLIVCDISGYGDTGPYASKKAYDLLVQSEAGVLSVTGTADTPSKV
                     GISIADIATGVYAYSGILTALYQREKTGKGGRVEVTMLEALGEWMGYPLYYAHFSGVA
                     PPRTGPDHATIVPYGCYQTGDGRSVMLGLQNEREWATFCDKVLGQPGLATDARYDTNF
                     KRNERREEVAALINKVFSTLTAEQLVEKLDEAGIANARMNTPEEVWQHAQLKARERWR
                     DVGSPVGEIPALLPPATHSGFDARMDPVPEIGEHTEHILAELGFKDSEIEALKSDAAI
                     SFERRKVK"
     gene            complement(13699..>14124)
                     /locus_tag="AC158Q_03760"
     CDS             complement(13699..>14124)
                     /locus_tag="AC158Q_03760"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011518414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3338916.1"
                     /translation="ILIAAECVGVVYWFIDRAKAYAKERVVFDLPIGQNQGIQFPIAR
                     AYASVQAADLMRYKAAALFDAGKPCGAEANMALLLGANASWEAANASLQTHGGFGFAA
                     DYDVERKFRETRLYQVAPISTNLILSYIGEHVLGMPRSF"
BASE COUNT         2530 a         4751 c         4414 g         2429 t
ORIGIN      
        1 gtagcggtag cggtgcaggg acttgtccgg cagccgtgag acgatttcgg attcgggaaa
       61 cagcctgccc gcccgctcga agatatgcgc gagcgtgagc ggaaactcca tcatcgtgga
      121 ttgcatatca gtcgtcgcga gccagtcgcc gcgagtcacc ggcatccata tccgccagca
      181 ggctcacgcc gtgcgccgcg aatcgccgcg cagccgcgcg cgcccgctga tccttctcgt
      241 tcatcagcga ccactgcgcg tggcgcacca gcagcgcgag ttccatgccg cgccccactg
      301 tcatggcgaa gcgccgcgcg ccggcttcta gttgcgcgcc cccggtcccg gaaagccatg
      361 ccccggcctg ctccatcacg cgctcgacct gcgccgagat cttcaagaga tccggctccc
      421 gcacctcgcg cagcaggata ccgatctccc gctggagcac cgccaagccg ccgacagcct
      481 ccagtgcgcg caaggtttcc agcgacagca cattggtcgt gccttcccag atcggcagca
      541 cctgcgcatc gcgcagcagc accggcagcc cggtgtcttc cacgtacccg gccccgccga
      601 aggcttcgat cacttccgag aggatctgta ccgcctgctt gccggtgaga agcttggcga
      661 ccggagtcag cacccgcagg agcttttcct gcccttctcc ggccttgccc gcctcctcgc
      721 gccccatcaa ctcggccacg aagaacgtga ggtgaagcgc cccaagaaat tcggcctcga
      781 gccccgccag cgtgtcaagg tgcagcggct tctcggagag cggggcaccg aacgcaatgc
      841 gcctggaggc gtaatcacgc gcgagcgcga gcccgcgcct cgcgaacgca caggcgctca
      901 ccgagttcca ggtgcgcgtg acgttgagca tgggcgcgat actcctcacg ccgtcgcggt
      961 cgcccgccac gagctgtgcc ggcacgccct cgagcgtcag ctccgccgtc ggcaccttgc
     1021 gcgtgcccag cttgtctttc aggcggttga cgagaatgcc gttgggcctg ccctcgccat
     1081 catgggtttc caggtaaaag agcgcgagac ccttgccgcc cgcggggttg ccctcgggcc
     1141 gcgcaagagt cagcgccatc tgcgagccga tcgccgaagt gaaccacttg cggccataaa
     1201 gccgccaccc atctccggtc ttccgcgcaa cggtctcggt cagacccacg tcggagccgc
     1261 cggccgcttc cgtcatccac tggccgctgg tccagaactc gcgcgggtca cggctcgtga
     1321 ggtgcggcac cgcccggtct atcagacgct ggttgcccgc ggcgagcagt gcccgcgccg
     1381 cgccgtcggt catggccagc ggacagctgt agagatcgtt cgaaggcgtg aaaagatagg
     1441 cgagcgcgaa ctggtggacg cgcgagtaca tgccatgccg ccgctcgtac gccgtggcga
     1501 ccagcccttg ctcggcagcg atgcgttcgg cctcgtgcca cagagccgtc acctcgatgc
     1561 gatccacgcg gttgccccac gcgtcccact gtctgagcgt tggctcgctt tcccggtctg
     1621 cgagctgatg ccgatagagt tcccccccgg ccagctcgcc cattgctgcg agggcgggct
     1681 cgacctccgt gaggacgtcg gccgggagca cccgcgccag atacgagcgc agagcgcggt
     1741 cggcggtgaa ctggttgcca agccccggag acggctgaac gaaggccatg gcctatctta
     1801 gccgtgagtt ctgcctcaat tccagccagc gcagcgccag cagcccggcg acgcccagga
     1861 tccccgccgc gatgcacgcg agatgcaggg tttccaggga cacgatgaga tgccacggag
     1921 aggcgccgga aaacggcgcg aacggctcca tgtccccgtg catgatgctg tcgatcgcga
     1981 cgtgactgta ggctcctatg aatgcacccg tgccggccgc cttcagggat atcccgggct
     2041 cgcaggccag ccaacgggcc tgggccgggc tcaaccaggc attccagagc cgcagcgccc
     2101 attcccccac gggccggccc gcccaccagc tcgcgataaa gatgagcgtc gcgccgagat
     2161 aggtatgcag gtacgggtgg acgggatcgc cggtgaaaag gaaaaacccg atcggctcca
     2221 ggtcgatcag cgcttgcgag aacgcgaaca ccataaaact gaaatgccgc ggaaccgccg
     2281 ccttgaccag ggctccgggg ccgaaatgaa aaggggttat cggcactaaa atgcggtgac
     2341 gagtgataag tgacgagtga cgagggtcac ccacgcaacg gcttcaggtc gagcggaccg
     2401 tcgaaaaatt tcggtaccga ggcaaggaaa cggtcggcct gctcgcgcga ccacccgccg
     2461 tattcgcaat tgccgcggaa cttcgtctcg atatcagcgc gagtaagcgg ctcttgcgcg
     2521 ccgccgcgta tatgaggctg gcgctcctcg agtacgcggc cgtccttcag cgtcatgcga
     2581 acgtggccgg tataggcctt cggatacggg ttgtgaggat ccacgacata gcgcaccttg
     2641 gaagccagcg cgagtatgcg ggcatccctt accgtctcct cggtgaacgc ggcgagcccc
     2701 gccccgccgg tcacgaacgc gacggcaata ttgaacggcg cgctgaattt ggcggcgtaa
     2761 gcgttcgccg ggcgatgctt ggaaggcagc gggtcccaca ggcggtgcac gtagccctcc
     2821 gccgtctcgc acagcacctc cttcacctcc tcgggctgca cgccgtgccg gcgcagccgc
     2881 agcgcgcagt cgatgtaggg ctggttcatg gtgcccgtgg cgtagggctt gaatgtgatg
     2941 tcccgcatga cccactcttc accgaaattg ccggtcagca caccatattt gccttcgacg
     3001 gagcgcgcga agccgtgaaa cagcccgtgg acgccctcca gcgcggtgcg cggcccgaga
     3061 aacccgccgc ggcctatccg cgccgccgcg atggatgact tggctgccca ccccgggtgc
     3121 aggcgcttgg tccacgcgcc ctccgccagg tactcgataa tgccgctggc caggctggcc
     3181 gaattgccga gcgcgtccac cgtctgccgg cgggtcagcc ccagcgccct cgacaccgcc
     3241 agcgtgccgc cgatcgtgcc cagcacgctg gtcggatgaa aacccgcttt gtggatcgcc
     3301 ttgggaatca ccatcgacag ccggcaggtc gcctcgatgc cggcaacgat accggtcagc
     3361 gccgcccgcc cgtcctgtcc aaagcgctcg cacgcggcga gcaccgccgg gatcacgacg
     3421 gcgctgctgt ggaccggccc gccttcaaac gtgtcgtcga aatcctcccc gtgcaccgcg
     3481 gtgccgttga tgagggcggc gtcctcggcg ctgtagccgg acggatgccc gatcgccgtg
     3541 cacggccccc cgccgtccat cgcgcccacc agcgcctgca tgtaatccgt gccccgggcg
     3601 gcgacacaca gtccgacgat atcgatcagg agctgctcgg cgcgggcgcg caccggcacc
     3661 ggaatactct ccgggccaag cgccgcagtt ttttcagcaa gaatttccgt tacggagggg
     3721 gcggcggaag gagcattcat ccgtattagt gatgggtaac gggacagggc gggatcctct
     3781 tccctccgcc ctcatccctt tcgttgcggc cgcgctgatc agcgcgcgac cttgacgccg
     3841 cccgctgtca gaatcttccg ggttgactcg gaaaactcat ccccgaattt ggcgagttcc
     3901 gcgctgcgca tgtatccgtc ctggaacatg ttgtcggcca ggtatttctg ccagctcggc
     3961 gtcttgatga aacgcgcgaa cagctcctcc cagtaggcaa cggcttcggg cggaatgccc
     4021 ggcggtgcga cgattccccg aacctggggc acgttgggaa tcttgaagcc cgcttcctgg
     4081 atcgtcggca catcggggaa cgccggcagg cgcttgtccg agactgtcgc gatcacgcgc
     4141 aggttgccgg cgcggatgtg ctcccccgct tcctgcggtt cgattaccat catgttgaca
     4201 tggccgccca gcaacgcggc gatgcgctcg ccgcctcccg ggaaggagat atacgcccaa
     4261 ctcgcgccgg tatgggtcat cagcaactgg cgcacgatcc agtcgcggct gccgagcgag
     4321 ccggccgact gcttcaactc ccccggtttc tgcttggccg catcgatgaa gtccttcagc
     4381 gtcttgtggg gcgagtcgct cttcaccgcg atcatcgccg gctcgagcac gaggcgaacc
     4441 accggcgtca gatccttcat cgtgaacttg gcttcctttc tcatgatcgg gatggtaaac
     4501 cagatgccgg tgaagacggc gatggtgtgg gtttcgccct tcttttcggc gaggtaggcc
     4561 atcgcggtca gtccaccgcc gccggtcttg ttcgccacct gcatgcgcac gggcatcagg
     4621 ccttccttct ccatcgcagc cgcgatgccg cgcgcgaaca cgtcgccgcc gccgcccggg
     4681 ccggtatggg tcacgaactc gaccggcttg ctgggcttga actgcgcgac cgccgggccg
     4741 gcggccagca tcggaaagac tgcggccatc aacagacaca ctgagcgaaa tttcatgttg
     4801 gactcctcct taaggtttat gggctgcctc atccgccttc ttccagcgcc ctgacgcgca
     4861 gtgcgttggc cttcttgaac atcggcgcaa gcagtatcaa caccgccaaa aatagcatcg
     4921 tcgccgatat gggtctagag accagtatag agagttcgcc ttgcgacatc atcagcgact
     4981 ggcgcagcgc ccgctccatg ctgtccccca gcaccagtcc gaggatcagg ggcgcagccg
     5041 gataatccag ccggcgcatc gcgtagccga gcaatccgaa aaccgtcagc agcccgacgt
     5101 cgaacaggct gctcgagacg ctgtagaccc cgaccatgga aatgccgaag atgaccggat
     5161 agaggacgta gaccggcagg cgcatgatct gcgcgaacag cgggatcaga ggcaaattga
     5221 gcaccagcag catcacgttg ccgacataca tgctcgcgac cagcccccag aacagcgtgg
     5281 ggttgtcctg gatcatcagc gggccgggcc tcagtcccca gagtatgaag gccgcgagca
     5341 ggatcgccgt ggtgccggaa ccgggaatcc ccagcgtgag cagcggcacc atggcgccgc
     5401 ccgtctcgga gttgttggcg ccctcgggcg ccgccacgcc ttccggcacg ccggtgccga
     5461 atttctccgg gtgcttcgac accgccttct cgatcccgta ggaaatgaag gacgcgatgg
     5521 tggaacccgc cccgggcagc acgccgatca ggaagccgac gaccgatccg ttgatgaaag
     5581 cgaaacgaca ggccttgagt tccgcccacg tcggcatcag gttgcgcagt cccctgggca
     5641 ccggcagcat cttgggcccg tcccggatct ccatgttgcc cagcacctcg ccgagcgcga
     5701 acaccccgat cgccaccacc acgaaatcga tgccgtcgag catgtgcatg ctgccgaagg
     5761 taaaacgcga ttccgacgtg aagagatccg tgcccatgcc ggcaatccac aggcccacca
     5821 gcatcgcgag cagcgccttg accagggact ttccggccag cagcaccacc atcgccaggc
     5881 ccaggcacat cagcgcgaaa tactccggcg aactgaaaga cagcgcgagt ttcgccagcg
     5941 gcggcgcgac cagcatgagg ccgatgatgc ccaccgtgcc ggccacgaag gaagcgatcg
     6001 cggcgatgcc gagcgccgga ccgccgcgcc cgtttttcgc catctggtag ccgtcgagac
     6061 acgtcaccac cgaggccgat tctcccggca cgttgagcag tatggacgtg gtcgaaccgc
     6121 catatttgga accgtagtag ataccggcga gcatgatgag cgcgccggtc ggctccatct
     6181 tgaaggtcag cggcagcagc atcgcgatgg tggccgccga gccgagcccc ggcagcacgc
     6241 cgatcacagt gccgaggaag acgccgatga agcaccacag gaggttgtac ggcgtcagcg
     6301 cgaccgcgaa tccctggtag agatggccga tgacttccat cagcttttcc ggaaagcgcg
     6361 gcgggtgaca ggtgacgggt gacggttgtt tcgcactctt gcctcttgcc ttgtgccttg
     6421 tcagaacccc agcttgccta tcggcagctc cacgttgagc gcgagcggga aaagcaggta
     6481 gaagccggcg gacatggcga ccgcaacggc aagggccatg cccggcgggc ggcggtacat
     6541 cacggcaatc aggaagaacg tgaacagcgc gaacgcgagc aggaagccca gcacctggag
     6601 cagggccacg cacagcgcga aggccgacca cgatgcgagc acccttccga cctcgcgcca
     6661 gtccgttgcg cccccgggtc ccgccgcagc ggcgccgagg ctcgttccca cgagcagcag
     6721 ggaaagcacg atcatgcacg cgccgtacca caacgggaaa aaccccggcc cgggtccatc
     6781 gaggccgcta tattccaggc cgcgcgcctc gacgacgatg aaaatcccga ggccggtcag
     6841 cgcgccgccg gaaacgatgt cgcccgtctt agccatcagc agtcagcaga tagctatcaa
     6901 tgaacagcag aaataatccg accgaatgcc actcgctgag cgccggtagc ggtgcttatg
     6961 ccgtcttggc tttcttgtag cgcttggccg gcacgatctc ggcgacgata ccgtcgggcg
     7021 cccggaactt gacgggaatg acgcccgggt cgctgacgat ctcgcagccg tactgcttca
     7081 ggccggcgac gctggcttcg acgtcttcca cttccaccgc gaagtgatgg atgcacggct
     7141 tctcgccggc cgccagggat tccgcggacc gcgtgccctc gtcatatttc atcagcgtga
     7201 agtcgagggt cccgtccgtc atatgccgcg agatatggtc ccgcgtcctc ttggtctcca
     7261 cttccctgaa gccgaaaacc ttctcgtaga actccgtggc tttctccagg ttctcgactt
     7321 tcagtgcaag gtgaacgatt cggctcatgt cggatccgtg aacaatgatt gatgatagga
     7381 caggtaccgc ttcaactatc gaattcgtag cctgccctgg cgagtaacgg cctggccgca
     7441 gcgctcgtca ggcgggtgat gaagtcgcgt gccgcacgcg gttcggcggc ccgggccgcc
     7501 agcccggccg cgtacaccgt catcgcctgc agatcgccgg gcagcggtcc cgcgtatgtt
     7561 acacccggat tggcaagaat ttccgtaatc tgggtaatcc ccaattcgta cggcgcatgg
     7621 ctgccagcca ggtactgcat ggcggcatag ccattgggga agaattgcca gtgcgatttc
     7681 atcgtgtcgg ccaggcccag gcgttccagt gcccgcatga ccaccttgcc ggccgtcgcc
     7741 gtggcaggat ccgggcaaac cagcctcgcg gcagcaagca ggttgccgcg cagcgcgcgc
     7801 tcgttcgaca cgtccggcaa tggcgtgccc gcgcgcaccg ccacacccgt gccgactttt
     7861 ccgaggtctg cgcgcgagcc ggcagcgacg tgtcccgcgg acgtcaactc atcgatcagt
     7921 gctgcggtga gaacgatgac gtctgccgcc tcgccgttca ccacgcgcgc attcattgcc
     7981 cccacggccc cgaagtcggc cttgacctcg tggccggttt ccgacgacca ggttgccgcg
     8041 atgggctcga tcaccgcctg tgccgcgccg gcgctcagaa ttcttaccaa agccatcgct
     8101 ccacccggcc tcacgtgcgt ggttcgtgaa tcggcgccgg ccgccgccga ctcgttcgcg
     8161 cgggaatcgt agcatacgcg tgcagcgccg cgcggcgatc cgttctgaag ttgagccgct
     8221 cccggggtga tgctagcctg tcgaaacagg acctgcggtc tacaaaggat agggggttcc
     8281 catgggtgtc gctcgcttct ttctggggtg cgtggccctg ttgccgggcg tgattcatgc
     8341 cgccgcgacg gaatcctttg tcaacgatat tgaaggacgc cccggaacgt ttacgcgggt
     8401 gctggtcgtc aagcccgaaa aggcggtggc gacggtgatc ctgttcgccg gcggcaacgg
     8461 cattctcaac atcgcgccgg atggcagcgt cggcaacagc ggcaatttcc tggtgcgcag
     8521 ccgggagagc ttcgcccaac accacctgat gacggcggta atcgatgccc cgtcggaccg
     8581 cctgccgtca cccgggctca gcgactttcg caagagcgcg ggacacgccc aggacatcgc
     8641 ggccgtgatc gccttcctgc gcaaggtggc aaacctgcct gtctggctgg tcggaacgag
     8701 ccgcggcacg acttccgcgg ccaatggcgc ggtcaggctg cagcagggcg ggcccgacgg
     8761 catcgtgctg acttcgagca tcgtcaagcc cgacgatacc ggcacgcttg ttacgatggc
     8821 cctcgagaag atcacggttc cggcactcgt ggtccatcac gcgaatgatg cctgcctcac
     8881 caccctgttc caggatctcc cgccggtcct ggccaagctc aagcttgcgc cgattgaagt
     8941 gcatcaactg acgatcaagg gcggcaagcc gccggaaccg ggcgcggacc cgtgcggccc
     9001 cttccattac cacggttatc tcgggatcga ggacgcagtc gtcgcccgca tctccggctg
     9061 gatcaagtcc ggcaagccct agcgccccct tgaagcaaat tcacgcggcc aagcacgcgc
     9121 ccgaagccca tctcgtgaag ggcttcctgg agtcgaacgg gattgccgcc gtggtgcgcg
     9181 gcgagttcct caccggcggc tggggcgagc tgcccgtgga cgtctgctcg gtctggatct
     9241 cggatgaccg ccagtacgac cgcgcgcaag ccttgctggt ggcgttcctg cgtggcgact
     9301 tcgcgactga atttcgcggg caagactggc gctgcccgaa atgcggcgaa cagctcgaag
     9361 gccagttcac cgcgtgctgg agctgcggga cgcgccgatc cgcctgaacg ggacggggac
     9421 acgacatgga tctcgagctc gcgggcctga ccgccctcgt caccggcgcc tcgcgcggca
     9481 tcggtctcgc cattgcacgg caactggcgc gagaaggctg caacctgcac ctggcggcac
     9541 gcaccgttgc cgacctggat gccgcacgtt cctcgatact cgggcagggc caggttgcgg
     9601 tcgaatgcca cgcgctcgac ctgggcagga gcgggaatgc cgcccggctc gcccagcgtt
     9661 gcggggatgt ggacatcctc atcaacaacg ccggctccat cccttcgggc cacctgctgg
     9721 agatcgacga ggcgcgatgg cgccagtcgt gggaactcaa ggtcttcggt tatatcaacc
     9781 tcacgcgcga ggtctaccgg acgatgcgcg cgcgccggcg cggggtcatc gtcaacgtca
     9841 tcggcgtcgc gggcgagcgc ctgcgccagg actatatcgc gggtggcacc ggcaacgccg
     9901 cgctgatggc gttcacgaga aacctcggcg gcgaaagcgt ggattacggt gtgcgggtcg
     9961 tgggagtcaa tcccgggcag atcgagaccg accggttgcg gcagcggctc gagcgcaagg
    10021 cgaaggaaga actcggcgat cccggccgct ggcgcgagct ggtcctcaat ccgccgttcg
    10081 gccggctcgc ccgggcggaa gaagtcgccg acacggtggt ctacctcgcc tccgcccgcg
    10141 cctcctacat cagcggcacc atcgtcaccg tggacggtgg ccgcgcagtg cgtaacgcaa
    10201 cctgatttca ggttcaaggt tcaacaactt ttgtcgtcgt accttgcacc ttgttcctgg
    10261 taccttgcgt cttcaaaaaa tatctccccg cagatattgc ggtggccacg gcgctttcgc
    10321 tttcagcacc ttggcggcat gtatcggcca gtacgggtcg ttgagcaacg cgcgcgccat
    10381 gatgatgatg tcggcctcgc cgcccgcgag aatctgctcg gccatgtcgg ggcttgagat
    10441 catccccacg gcgcccgtgg cgatgccggc gcggctgcgg atcgccgcgg agaacggcac
    10501 ctggtagccc ggaaaggcct ttaccttctg gcgggggtcg acacccccgc tcgagcagtc
    10561 gatcaaatcc accttgccgc cggccttgag caactgagca agcttcacgc tcgcttcaat
    10621 atcccagccg ccctccatcc agtcggtgca ggagatgcgc acgaaaagcg gcttgtcctc
    10681 cggccattcc gatcgcactg cgtccacgac ctccagcaga aaacgcgtgc gattatcgaa
    10741 actgccgccg taacgatcgg tgcggcggtt cgagatcgga gaaagaaacg aggaaatcag
    10801 gtagccgtgg gcgccgtgca actcgatgac ttcgaaaccc gcctcgcggg cacgccccgc
    10861 cgcggcggcg aacaatgcta ccgtgccggc gatggttttt tcgtccattt cctgcggcac
    10921 cgggaagcct tcgccgaaag gcactgccga cggggcgatg atggtccatc cgccctcgtc
    10981 cggcgccagc cccctgccgc cttcccacgg acgcatggtc gaggccttgc gccccgcgtg
    11041 tgcgagctgg atgcccgcta ccgcgccctg tgatttgacg aaccgcgcga tgcgctccag
    11101 cgcatcgcgc tgcgcatcgt tccacagacc catgcatccc gcggtaatac gcccccgcgg
    11161 ctccacgttc gtcatttcca cgaaaacgaa acccgccccg ccgaccgccc gcgaagcgag
    11221 gttctggaag tgccagtcgt tcgccacccc gtcgatggcc gagtactggc acatcggcga
    11281 aaccatgatg cggttgcgcg ccgttacgga gcggaaacgg attggctgga agagacgggg
    11341 aatcgagtcg tgctgaaaaa tcatgacggg ttattttagt gcatagctga tgaaggaccc
    11401 gttagaattc tcgtgtccgc ggcacttatc catccaagga gcagcccgtc atgcgcaaac
    11461 cgatccgcac cgacatcgcc tggagcacgc cctccaggat catagtcaag ggctacgacc
    11521 tttgcaggga cgtcctcggc acattgtcgc tcggcgacat ggcattcctg gagctgaccg
    11581 accgcctgcc aacggcacgg gaatcagtgg tgttcaatgc cattgcggtg acgctggtcg
    11641 agcatggcct caccccgagc gccatcgcgg cccgcatgac ctatactggc gcgccggagg
    11701 cgatgcaggc cgctgtcgcc gccgggttgt gcgggctggg cagcgtgttc gtcggcagca
    11761 tggaaaccgc tgcgcggatg ctgcaggagg ccatgcccga tccggcgaat gaagcggatt
    11821 tcgacaagct tgcgcgtgag atcgtggaac gttttcacgg ccgcaagcag ccggtgccgg
    11881 gtatcggcca ccccctgcac aaacccgagg acccgcgcgc gccgcggctg ttccagatcg
    11941 cggccgagaa cggattcgac gggccttaca tcaagctgat gaagcaggtg gcgcaggagg
    12001 ccgggagtgt ttatggaaaa tcactgccga tcaatgcgac cggcgccatc gcggcgattg
    12061 ccagcgagct caagctgccg tggaaaatcg tgcgcggtat cggcgtgatg gcgcgcgcga
    12121 tcgggctggt cggtcacatt cgcgaggaga tgcgcgcgcc gatggcccgg gaaatcaagg
    12181 cctgggcgga ggaagaagcc accgcccacc tcaggaccaa ggcgtaatta gtaaatcctg
    12241 attcgtgatt cgtcccggga gcgccccgtt gcagcgcctc atccctttgc ccgactcacg
    12301 actcacgatt cacgcagtta cttgactttg cgccgctcga acgagatcgc cgcatccgat
    12361 ttgagcgctt cgatctcgct gtccttgaaa ccgagctcgg cgaggatgtg ctcggtgtgt
    12421 tcgccgattt cggggacggg gtccatgcgg gcatcaaatc cggaatgcgt cgccggcggc
    12481 agcagcgccg ggatctcccc taccggcgag ccgacgtcgc gccagcgttc gcgcgccttg
    12541 agctgcgcgt gctgccacac ttcttccggg gtgttcatgc gcgcgttggc gatgcccgcc
    12601 tcatcgagct tttcgaccag ctgttccgcc gtcagtgtcg aaaacacctt gttgatgagc
    12661 gccgcgacct cctcgcgccg ctcgttgcgc ttgaaattgg tgtcgtagcg ggcatcggtc
    12721 gcaagcccgg gctggcccag gaccttgtcg cagaacgttg cccactcccg ctcgttctgc
    12781 aggccgagca tcacgctcct gccgtccccg gtctggtagc agccgtaggg cacgatggtc
    12841 gcgtggtcgg ggccggtgcg cggcggggcg accccgctga aatgggcgta gtagagcggg
    12901 tagcccatcc actcgccgag cgcttccagc atcgtcacct cgacgcgccc gcccttgccg
    12961 gttttctcgc gctggtagag cgcagtcagt atgccggaat aggcataaac gccggtcgcg
    13021 atgtcggcga tcgagatccc gaccttgctg ggcgtatccg ctgtgccggt cacggacagc
    13081 acgcccgcct cgctctgcac cagcaggtcg tatgctttct tgctggcgta cggcccggta
    13141 tccccgtagc ccgagatgtc gcacacgatg agcctgggat gtctttccag cagcccctcc
    13201 gcctccaggc ccaggcggga agccgcgccc ggcgcaaggt tttggacgaa aacgtcggcg
    13261 cgcgcgatca gccgctccag gatgtctttc gccttcgagt gcttcacgtc aagcgtcagg
    13321 ctttccttgc tgcggttcaa ccacacgaag tgggaagcca ttcccttcac cgtgcggtcg
    13381 tagtcgcgcg cgaaatcgcc tactttaggg cgctcgatct tgatcaccct cgcgccgaga
    13441 tcggcgaggt gacgcgtcgc gaacggagcg gctaccgcct gctccagcga caccactgta
    13501 attccatcta aaggacgcat gaagagccgg aaaatagccg ccaagacgcc aagaacgcca
    13561 aggaaaacaa atcaacaatg aaaaccaaac ccgtcattac cattttcttc gcctgcgctc
    13621 gcgcaagcca gagcacttgt agtggttcta attatcttgc tgtgtctttc ttggcgtcct
    13681 tggcgtcttg gcggctaatt aaaacgatct tggcatgcct aagacatgct cgccgatgta
    13741 cgacaggatc aggttggtgg aaatcggcgc cacttggtag agccgcgtct cgcggaactt
    13801 gcgctcgacg tcgtagtccg cggcgaaacc gaagcccccg tgggtttgca ggctggcgtt
    13861 tgccgcctcc catgacgcat tggcgccgag caacagcgcc atgttggctt ccgctccgca
    13921 gggcttgccg gcatcgaaga gcgccgccgc cttgtagcgc atgagatccg cggcctgcac
    13981 gctcgcgtag gctcgcgcga tcgggaactg gatcccctgg ttctggccga tgggcaggtc
    14041 gaaaacgacc cgctccttag cgtaagcttt ggccctgtcg atgaaccagt agacgacgcc
    14101 cacgcactcg gcggcgatca ggat
//
LOCUS       DBNGSG010000143         4345 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1747733, whole genome shotgun sequence.
ACCESSION   DBNGSG010000143 DBNGSG010000000
VERSION     DBNGSG010000143.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4345)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1747733"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="AC158Q_03765"
     CDS             complement(<1..398)
                     /locus_tag="AC158Q_03765"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3338917.1"
                     /translation="MFSLKDKVALVTGSSRGIGKAIIEQMAAAGARVVVSSRKPEVCE
                     AVRAEFAARGQEAIAVPCNVGNKAELAKLVDTVMAQWGRIDILVCNAATNPVYGPMAS
                     ASDEAFDKIMLTNVKSVFWLAGMVLPQMAGR"
     gene            complement(561..1580)
                     /locus_tag="AC158Q_03770"
     CDS             complement(561..1580)
                     /locus_tag="AC158Q_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0006569 - L-tryptophan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR3338918.1"
                     /translation="MAKRWKQRPEGAQWGEFGDDDQRGRMNLVTDAVRLRAAREVKSG
                     RAFCLSHPLDRPGGNVLNASRFEPVFHPVRRDGLLYFNLPFEKIDPRFTDVGSDEAVM
                     FYTQYSTHWDGFPHKGMLFDVDGDGAEEKVFYNGYAIVDGAGRGTQGELGAVSLGIAN
                     MAETGVQGRGVMIDLRHHFGDKRVAVDFDMLMQVLKADGVEVEKGDIVCIHTGLGQLI
                     RDADGKLGEPIRHACTVIDGYDKKLLEWIADTGIAAIASDNLAVERSSTLGADPRLPH
                     RGPALPLHQHCLVKLGIHLGELWYLTELAAWLRENRRSRFLLTAPPLRLPGAAGSPVT
                     PVATV"
     gene            complement(1697..1891)
                     /locus_tag="AC158Q_03775"
     CDS             complement(1697..1891)
                     /locus_tag="AC158Q_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338919.1"
                     /translation="MSKLLSLFLLIPSFALAAAEDQVASPPAEGVSMVYVVLFAVVFF
                     GMIAGFFIYLWWNEKHKKPE"
     gene            2218..3225
                     /locus_tag="AC158Q_03780"
     CDS             2218..3225
                     /locus_tag="AC158Q_03780"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HIR3338920.1"
                     /translation="MKGSQFRLKHVLTALCAAALAFAAQHAAAQAASTGSGQAFPAKN
                     VQMIVPYTPGGSADLFSRVMAQKLGEAWGRSVIVENRPGASGMIGTEIVTKAAPDGYM
                     LLGHTSSYPATASVRAKLPFDPARAIIPVAMIARAPMIIAVHPSVPAKNVKELIAVAK
                     RTKGGLNYGSSGSGGNNHFAASLFAAAAGIQMVHVPYKGISIAVTALASGEIEVLISS
                     RAALAPQIQANRVRILAVTSEGPSPLFPDLPPAAKDAPGFSYQLWWGLFAPAGMSAER
                     LAFINATANKIIASDDMQKLITREAAEPWPLTVKELDGLLVREIDRYKKAAQIAGIKP
                     Q"
     gene            complement(3573..>4345)
                     /locus_tag="AC158Q_03785"
     CDS             complement(3573..>4345)
                     /locus_tag="AC158Q_03785"
                     /inference="COORDINATES: protein motif:HMM:NF041278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CmcJ/NvfI family oxidoreductase"
                     /protein_id="HIR3338921.1"
                     /translation="TGEKLVNVTFGPNNIRRRTAGTEEIHEVEIRNGRVSSTRFSLDE
                     NGFVLVEHRTAVRDFFDKAELERVYYPEVAALVRSLSGAKRVHVFDHTLRSGDEAERE
                     ARLVREPVLSAHNDYTEWSGPQRVRDLLPDEAEELLKRRFAIIQVWRAINQPIESNPL
                     AVADASSVAFTDFLVAERKYPDRVGQTYRLKHNPNHRWYYFPHMRRDEAIVFKVYDSA
                     KDGRARFTPHTSFDDPNSPPGARPRQSIEARALAFFSS"
BASE COUNT          766 a         1437 c         1375 g          767 t
ORIGIN      
        1 cgcccggcca tctgcggcag caccatgccg gcgagccaga acacgctctt cacgttggtc
       61 agcatgatct tgtcgaacgc ctcgtccgac gcgctggcca tcggcccgta gaccgggttg
      121 gtcgccgcgt tgcagaccag gatgtcgatg cggccccatt gggccatcac ggtatccacc
      181 agcttcgcga gctccgcctt gttgccgaca ttgcacggca ccgcgattgc ctcctggccg
      241 cgcgcggcga attcggcgcg caccgcctcg cagacttccg gcttgcggct cgacaccacg
      301 acccgggccc cggcagcggc catctgttcg atgatcgcct tgccgatgcc ccgcgacgag
      361 ccggtcacca gggcgacttt gtctttcagg ctgaacatgc gcgtgactcc cctcttcgcg
      421 gctcaaaaag tgattcgttt ctcgtttctc gctgctcgac tcttctgact acaccccaaa
      481 cggcgtggat gggggttggt ggacggtttc ccgtgccgcg agcctcagcc cttttgcctt
      541 gtgccttgtg ccttgtgcct tcagacggtc gcgaccgggg tgaccggcga gcccgccgcg
      601 ccgggcaggc gcaagggcgg cgcggtcagc aggaagcggc tgcggcggtt ctcgcgcagc
      661 catgccgcca gttcggtgag ataccacagt tcgccgagat ggatgcccag cttgaccagg
      721 cagtgctgat gcagcggcag cgccggaccg cgatgcggca gccgcggatc cgcgccgagc
      781 gtggaggagc gctccacggc gaggttgtcg gaagcgatcg ccgcgatccc ggtgtcagcg
      841 atccactcca gcagtttttt atcgtagccg tcgatcacgg tgcaggcgtg gcggatgggc
      901 tcgccgagct tcccgtcggc gtcccggatc agctgcccga ggccggtgtg gatgcagacg
      961 atgtcgccct tctccacctc gacgccatcc gccttcagga cctgcatgag catgtcgaaa
     1021 tccaccgcga cgcgcttgtc gccgaaatga tggcgcagat cgatcatcac tccccgcccc
     1081 tgcacgccgg tctcggccat gttggcgatg ccgaggctga ccgcgcccag ctcgccctgg
     1141 gtgccgcggc ccgctccatc gacgatcgcg tagccgttgt agaacacctt ttcctcggcg
     1201 ccgtcgccgt cgacgtcgaa cagcatgccc ttgtgcggga agccgtccca gtgcgtcgag
     1261 tactgcgtgt agaacatgac tgcctcgtcg gaaccgacgt cggtgaagcg cgggtcgatt
     1321 ttctcgaagg ggagattgaa gtacagcagg ccgtcgcgcc tcaccgggtg gaacaccggc
     1381 tcgaagcggc tcgcgttcag cacgtttccg cccggccggt cgagcgggtg ggagaggcag
     1441 aacgcgcgcc cggacttcac ttcccgcgcc gcccgcagcc gcaccgcgtc cgtcaccagg
     1501 ttcatccgcc cgcgctggtc gtcgtccccg aattcacccc actgcgcccc ttccgggcgc
     1561 tgcttccagc gtttcgccat gagatcggtg ctccggaaaa accgtcatca taatcccagg
     1621 gagttcaggc cagagacact tagagcaaca aaaaaccccg ccgtggcggg gtttcagtgg
     1681 tgatggcgga gcgccgctat tccggcttct tgtgcttctc gttccaccag aggtagatga
     1741 aaaaaccggc gatcatgccg aagaagacga cggcaaacag cacgacgtaa accatgctca
     1801 ccccttcagc cgggggggag gccacctggt cttccgcggc ggcgagcgcg aaagagggaa
     1861 tcaacaggaa caaggacagt aatttactca taggactcca aatgctcact cgcgcaaccg
     1921 agggaccggg gcgatcccgt caccactcga accgggcggc agcgtgtgaa gagacgtccg
     1981 gtgcggcagt gtctcacgcc aggcttacgc cgggcttact tggccggaaa taaagtccat
     2041 actctggcgc atacccggca atgaaagcgc gaaacgggcg gctttagtcc atattatgcg
     2101 aatgcagccg ccggcttctg ggtggcggcg gccgcgcggg gtagaatcgc ggcctgcagc
     2161 agtgatcgcc cgaccctcag cagggccagt caatccgaca gagatggagg aagtttcatg
     2221 aaaggttcgc aattccgctt gaagcacgtc ctcaccgcgc tgtgcgccgc cgccctggca
     2281 ttcgccgcgc agcacgccgc ggcgcaggcc gcttcgacag gctcaggaca ggccttcccc
     2341 gcgaagaacg tccagatgat cgtgccctac acgccgggcg gctcggccga tctcttcagc
     2401 cgcgtcatgg cccagaagct gggtgaggcg tggggccgct ccgtcatcgt cgagaaccgc
     2461 ccgggcgcga gcggcatgat cggcaccgag atcgtcacca aggccgcacc cgacggctac
     2521 atgctcctcg gccatacgtc ttcctacccg gccaccgcct cggtgcgggc caagctgccg
     2581 ttcgacccgg cccgggccat catcccggtc gcgatgatcg cgcgcgcgcc gatgatcatc
     2641 gcggtgcatc cttcggtgcc ggccaagaac gtgaaggaac tcatcgcggt cgccaagcga
     2701 acgaagggcg ggctcaacta cggatcgtcg ggctcgggcg gcaacaacca cttcgccgca
     2761 tcgctgttcg cggcggccgc cggcattcag atggttcacg ttccgtacaa gggcatctcg
     2821 atcgccgtca ccgcgctcgc ctcgggggag atcgaagtgc tgatttccag ccgcgccgcg
     2881 ctggcgcctc agatccaggc gaaccgcgtg cgcatactgg ccgtcaccag cgaggggccc
     2941 tcgccgctgt tcccggacct gccgcctgcg gccaaggatg cgccgggctt cagctaccag
     3001 ctgtggtggg gactcttcgc tcccgcgggc atgtccgccg agcgtctcgc cttcatcaac
     3061 gccaccgcca acaagatcat cgcttccgac gatatgcaga aactgattac ccgcgaggca
     3121 gccgagccgt ggccgctcac ggtgaaggag ctcgacggtc tgctcgtgcg tgaaatcgac
     3181 cgctacaaga aagcggcgca gatcgcgggc atcaagccgc agtagcctgc ggcgtggcga
     3241 gtgtcgagtg gcgggtgacg agggacgggt gacaggtgac gggtgacggg tgacgaacgg
     3301 ccgttcaggc gattttaact cccgtcaccc gcaccaggct taacaggctg ttgaaataca
     3361 gctgtcactc ccgcgaaggc gggagtccag aaaacctgcc ttaatacgat tcaacgagtt
     3421 actggattcc cgcctccgcg ggaatgacgt tgttgctcta cgtaacgtat ttttcaacac
     3481 cctgctaact caccaccgtg cagtgtgaag ggggcaggtc ttgcgctagc gtaagtcatg
     3541 gcaagacctg gcctcatttc tccccggcgc tctcaggagc tgaagaaagc gagagcccgc
     3601 gcctcgatgc tctggcgcgg gcgcgcgccc ggcggcgaat tggggtcgtc gaaagaggta
     3661 tgcggggtaa agcgtgcccg cccatccttc gccgaatcgt agaccttgaa aactatcgcc
     3721 tcgtcccgcc gcatatgcgg gaaatagtac cagcgatggt tgggattgtg cttcagccgg
     3781 taggtctgcc cgacccggtc gggatatttg cgctccgcga ccagaaaatc ggtgaacgca
     3841 acgctggaag catcggcgac cgcgagcgga ttcgactcga tcggctgatt gatcgcccgc
     3901 catacctgga tgatcgcgaa gcgccgcttg agcagctcct ccgcttcgtc cggcaggaga
     3961 tcgcgcacgc gctgcggacc cgaccattcg gtgtaatcgt tatgcgcgga cagcaccggc
     4021 tcgcgcacca ggcgggcttc ccgttcggcc tcgtcgcccg agcgcagcgt gtgatcgaag
     4081 acgtggacgc gcttcgctcc cgagaggctc cggaccagcg ccgcaacttc cgggtagtag
     4141 acgcgctcga gctcggcctt gtcgaagaaa tcccggaccg cggtcctgtg ttcgaccagc
     4201 acgaagccgt tctcgtcgag ggaaaaccgg gtggaggaaa cgcggccatt gcgtatttcc
     4261 acctcatgga tttcttcggt gcccgcagtg cggcgccgga tgttgttcgg gccgaaggtc
     4321 acgttgacga gcttttcgcc ggtgg
//
LOCUS       DBNGSG010000144         3224 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3276064, whole genome shotgun sequence.
ACCESSION   DBNGSG010000144 DBNGSG010000000
VERSION     DBNGSG010000144.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3276064"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /gene="zapE"
                     /locus_tag="AC158Q_03790"
     CDS             <1..511
                     /gene="zapE"
                     /locus_tag="AC158Q_03790"
                     /inference="COORDINATES: protein motif:HMM:NF015902.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HIR3338922.1"
                     /translation="RALQQVKVYHTPITAQTDGSLMEAFKRLAEVEEERHELDVEGRV
                     IAYRHRAGGVVWFDFKVICGWGRSQNDYIDLAKRFHTLIVSGVPRMGPERADEARRFT
                     LLVDVLYDQRVKLIVSAEALPEALLDRSTGAGDSQLQAMVFQFDRTASRLAEMQTLDY
                     LNQPRVRQE"
     gene            511..1497
                     /locus_tag="AC158Q_03795"
     CDS             511..1497
                     /locus_tag="AC158Q_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR3338923.1"
                     /translation="MNPFKSLRVFDENGKVTARVVEVALSELSAGEVVFQTAYSSVNY
                     KDALAVTGTGKIIRSFPRIAGIDATGTVISSADGRVRTGDEVICTSYEFGVGQDGGFS
                     QVCRVPADWVVPLPKGLTLFDAAALGVAGYTAGLAIELLELNGMAPGNGKVLVNGATG
                     GVATLAIDMLAALGYHVVAVTGKDAEHGYLQKLGAKEVLSRQKLEMGTRPLEKGLWAA
                     AFDSVGGEQLAWLTRTMQQDGLIASFGNAGGIEFKTTVLPFILRGVRLIGVNSGDTPM
                     LLRRKVWGRLASDLKPQHISDIAHIIKLEDLPGYFERMLKGQIRGRAVVSMS"
     gene            1603..>3224
                     /locus_tag="AC158Q_03800"
     CDS             1603..>3224
                     /locus_tag="AC158Q_03800"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019543 - propionate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HIR3338924.1"
                     /translation="MGAYREFHQRSIDHREEFWAEQARLIDWHKPFGKVLDYSRPPFA
                     KWFVGGLTNLCHNAVDRHLAARGGQKSLVYISTETGQEKSYTYRELATEVNRCAAMIR
                     SLGVGKGDRVLIYMPMIPEAVFAMLACARLGAIHSVVFGGFASHSLAARIDDARPRLM
                     ITADGGMRGGRVILYRAMYDEAIRLSKSPPARVIVFNRGLDKELKLTPGRDLDYGALR
                     EKHLDEQVPVTWLESSDPSYILYTSGTTGQPKGAQRDTGGYAVALAASIKHIYCGEPG
                     ETMFTTSDIGWVVGHSYIVYGPLICGMTTILYEGLPIRPDAGIWWKIVADNKVSVMFS
                     SPTAARVLKKQDPVFLKKYDVSSLKHLFLAGEPLDEPTHRWFADALGKPVIDHYWQTE
                     TGWPILSSVPGIERTALKFGSPSFPVFGYDLRLLRETDATEAGPNEKAVVAIIPPLPP
                     GCMSTVWGDDERFVKTYFGSFRDRLAYSTFDWGIRDEEGYHFILGRTDDVINVAGHRL
                     GTREIEEAVQAHPNIAEVAVVGVADPIKGQMPL"
BASE COUNT          583 a          997 c         1071 g          573 t
ORIGIN      
        1 gcgcgcgctg cagcaggtca aggtctatca cacgccgatc acggcccaga ccgatgggtc
       61 cctgatggag gctttcaaga gactcgctga agtggaggag gagcggcacg agctcgacgt
      121 ggaaggccgc gtcattgcct accgtcaccg cgcgggcggc gtggtgtggt tcgatttcaa
      181 ggtgatttgc ggctgggggc gctcgcagaa cgattacatc gatctcgcca agcgcttcca
      241 caccctcatt gtctcggggg tgcccagaat ggggccggag cgcgccgacg aggcccgccg
      301 cttcacgctg ctggttgacg tgctctacga ccagcgcgta aaactgatcg tgtcggcgga
      361 ggcgctgccc gaggcgctgc tcgatcgcag caccggcgcc ggcgattcgc agttgcaggc
      421 gatggtcttc caattcgacc gcaccgcgag ccgcctcgcc gagatgcaga cactcgatta
      481 cttgaaccag ccccgggtga gacaggaata atgaatccat tcaaatcgct gcgcgtgttc
      541 gacgaaaacg gcaaggtcac cgcccgcgtg gtggaggtgg cgttgagcga attgtccgcg
      601 ggtgaagtcg tgttccaaac ggcctattcg agtgtcaact acaaggacgc acttgccgta
      661 accgggaccg gcaagatcat ccgcagcttt ccccgcatcg ccggaatcga cgcgaccggg
      721 accgtgatct cgtccgccga cgggcgcgtc cggaccggcg acgaagtgat ctgcaccagc
      781 tacgagttcg gcgtcgggca ggacggcggt ttttcgcagg tctgccgggt gcctgcggac
      841 tgggtggtgc cgctgcccaa aggactgacg ctgttcgacg cggcggcact cggcgtcgcg
      901 ggttataccg ccgggctggc gattgagcta ttggagttga acgggatggc gcccggtaat
      961 ggcaaggtgc tggtcaatgg cgctaccggc ggcgtggcga cgctcgccat cgacatgctg
     1021 gccgcgctgg gttaccacgt cgtcgcggtg accgggaagg acgcggagca cggctacctg
     1081 cagaaactgg gcgcgaagga agtgctatcg cggcagaaac tggaaatggg gactcgtccg
     1141 ctggagaaag ggctgtgggc ggcggcgttc gactcggtgg gcggggagca gcttgcgtgg
     1201 ctgacgcgca ccatgcagca ggacggcctc atcgcgagct tcggcaatgc cggcggcatc
     1261 gagttcaaga caacggtgct gccgttcatc ctgcgcggcg tgcgtctgat cggggtgaac
     1321 tccggcgaca cgccgatgct gctgcggcgc aaggtttggg ggcggctcgc gtccgacctc
     1381 aagccgcagc atatcagcga tatcgcgcac atcatcaagc tcgaagacct cccgggttac
     1441 ttcgagcgta tgctgaaagg acagatccgg gggcgcgccg tcgtcagcat gtcgtaagga
     1501 tcgacgacgc cttgcgctgc atcaaacccg gctgcgcaaa cgcccataga atggcacttg
     1561 ccggcaggcg ccggtagacg ctcgctttcg ggagggggag ccatgggtgc ttaccgcgag
     1621 ttccaccagc gatccatcga tcaccgggaa gaattctggg cagagcaggc ccggctcatt
     1681 gactggcaca agccgttcgg caaagtcctc gattacagcc ggccgccgtt cgccaaatgg
     1741 ttcgtcggcg ggctcaccaa cctgtgccac aacgcggtgg atcgccacct tgcggcgcgg
     1801 ggcggccaga agtcgctggt ctatatctcc accgagaccg ggcaggaaaa atcttatacc
     1861 taccgggaac tcgccaccga ggtcaaccgc tgcgcggcca tgatccgctc gctcggcgtg
     1921 ggcaagggcg accgcgtgct gatctacatg cccatgattc cggaagcggt gttcgcgatg
     1981 ctcgcctgcg cgcggttggg cgcaatccat tccgtcgttt ttggcgggtt tgcctcgcac
     2041 agcctggccg cgcgcatcga cgatgcacgg cccaggctga tgatcaccgc cgacggcggt
     2101 atgcgcggcg gacgcgtcat cctgtaccgc gccatgtacg atgaggcgat ccggctttcc
     2161 aaatcgcctc ccgccagggt catcgtcttc aaccgcggac tcgacaagga gttgaagctc
     2221 acgcccgggc gcgatctcga ttacggtgct ttgcgcgaga agcacctgga cgagcaagtg
     2281 ccggtcacct ggctcgagtc gagcgatccg tcctacattc tctatacttc gggcacgacc
     2341 ggccagccca agggcgcgca gcgtgatacc ggcggttatg ccgtggcgct cgccgcgtcc
     2401 atcaagcaca tctattgcgg tgagccgggg gagaccatgt ttaccacctc cgatatcggg
     2461 tgggtggttg ggcattcgta catcgtctac gggccgctga tctgcggcat gacgacaata
     2521 ctctacgagg ggctgccgat ccgtcccgat gccggcatct ggtggaagat cgtcgccgac
     2581 aacaaggtga gcgtgatgtt ctcctcgccg acggcggccc gcgtgttgaa gaagcaggac
     2641 ccggtgtttc taaaaaagta cgacgtgagt tcgctcaagc acctctttct cgctggcgag
     2701 ccgctcgacg agcccacgca ccgctggttc gcggacgcgc tgggcaagcc cgtcatagac
     2761 cattactggc agaccgagac cggctggccc attctctcca gcgtgccggg catcgagcgg
     2821 accgcgctca agttcggcag cccgagcttc ccggtattcg gctatgacct gcggctgctg
     2881 cgcgagaccg atgccaccga ggccggcccc aacgagaagg cggtggtggc gatcatcccg
     2941 ccgctgccgc cgggttgcat gtccaccgtc tggggcgacg acgagcgatt cgtgaagacc
     3001 tatttcggaa gctttcgcga caggctcgcg tattcaactt tcgactgggg catccgggac
     3061 gaggagggct accatttcat cctcggccgc accgacgacg tgatcaacgt cgcggggcac
     3121 cgcctcggta cgcgcgagat cgaagaggct gtccaggcgc atcccaacat tgcggaagtc
     3181 gctgtggtcg gggtcgccga tccgatcaag ggacagatgc cgct
//
LOCUS       DBNGSG010000145         5533 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4125534, whole genome shotgun sequence.
ACCESSION   DBNGSG010000145 DBNGSG010000000
VERSION     DBNGSG010000145.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5533)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5533)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5533
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4125534"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="AC158Q_03805"
     CDS             complement(<1..715)
                     /locus_tag="AC158Q_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3338925.1"
                     /translation="MGLPVRTFDAVIVGGGGSGMRAALELARADLKVAVLSKVFPTRS
                     HTVAAQGGVAASLGNIDEDNWHWHMYDTVKGSDYLGDQDAIEFMCRKANEAVYELEHF
                     GMPFDRLASGKIYQRPFGGHMSEYGKSPVPRACAAADRTGHALLHTLYQANVRARTQF
                     FVEWMALDLVRGQDGAVLGVTALEMETGEVMILQAKATVFATGGAGRIFSASTNAFIN
                     TGDGLGMAARAGIPLQDMEF"
     gene            complement(716..1063)
                     /gene="sdhD"
                     /locus_tag="AC158Q_03810"
     CDS             complement(716..1063)
                     /gene="sdhD"
                     /locus_tag="AC158Q_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HIR3338926.1"
                     /translation="MVKRDVVGAHYGLRDWLAQRVTAVIMAVYTVLILSVIAGLPKMD
                     YWQWKVVWQTPIVKYATVLFLLSLCLHAWIGVRNIFMDYIKDPTLRLTLYVLVISALV
                     WYGIWAVQILWSL"
     gene            complement(1057..1431)
                     /gene="sdhC"
                     /locus_tag="AC158Q_03815"
     CDS             complement(1057..1431)
                     /gene="sdhC"
                     /locus_tag="AC158Q_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HIR3338927.1"
                     /translation="MSRPRPKYLNLFQIRLPVPGLISILHRVSGAALFLFIPFLLTLF
                     EWSLESQQTFLRFKGTFSHWAIKLILIGLIWAYLHHLLAGLRHLALDLHYGTDLASGR
                     AISWAVLAGGIVLTLLIAVMIW"
     gene            1748..2737
                     /locus_tag="AC158Q_03820"
     CDS             1748..2737
                     /locus_tag="AC158Q_03820"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HIR3338928.1"
                     /translation="MKRPMRVAVTGAAGQIGYSLLPRIAAGEMLGRDQPVILQLLEIP
                     DEKAQKALRGVMMELDDCAFPLLHGMVPAADPMVAFKDADVALLVGARPRGPGMERKD
                     LLEANGKIFAPQGRALSEVASRKVKVLVVGNPANTNCLIAMKNAPWLKPSNFSSMMRI
                     DHNRALTQVAQKIGLPVTAIRKMTVWGNHSATQYPDLFQATAGGKKAWPMINDQSWLE
                     NTFIPVIQKRGAAIIEARGLSSAASAANAAIDHVRDWVKGTKAGDWTSMGIPSDGSYG
                     IAEGVLYGYPVTCKNGQYKIVKGLEVSEFSRGRMTATLKELHEERDSIKHLLG"
     gene            2811..3962
                     /gene="prpC"
                     /locus_tag="AC158Q_03825"
     CDS             2811..3962
                     /gene="prpC"
                     /locus_tag="AC158Q_03825"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036440 - citrate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019543 - propionate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate synthase/citrate
                     synthase"
                     /protein_id="HIR3338929.1"
                     /translation="MSEAQAGAKPKKSVALSGIAAGSTALCTVGRSGNDLHYRGYDIL
                     DIADQCEFEEIAHLLVHEKLPTRAELSSYKKKLGSLRGLPAPVKTTLEQIPASAHPMD
                     VLRTGCSALGAVAPEKDDHGIAGARDIADRLMASFGSMLLYWHHYSRDRRRIEVETDD
                     DSIGGHFLTLLHGRPSKPLWIKSMHTSLNLYAEHEFNASTFAARVVAGTGSDMHSAVT
                     GAIGTLKGPKHGGANEVGFEVMNRYESPDAAERDIVERIRNKEVVIGFGHPVYTIADP
                     RNKIIKEVARRLSQDAGEMEMFEIAERIEQVMMREKKMFANLDWFSAVSYHRMGVPTA
                     MFTPIFVIARTSGWAAHVIEQRIDNKIIRPTAVYTGPEPRKFIPMDQRK"
     gene            3959..5401
                     /locus_tag="AC158Q_03830"
     CDS             3959..5401
                     /locus_tag="AC158Q_03830"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019543 - propionate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate
                     dehydratase/aconitate hydratase"
                     /protein_id="HIR3338930.1"
                     /translation="MSSHISNVRAAPDKVLTDITDYVAGRAINSNEAYNTARLCLMDT
                     LGCGLEALEYPACTKLLGPIVPGAVLPNGAKVPGTPHQLDPVTAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRTRLARGGKPLLMRDVLTGMIKAHEIQGVIAL
                     ENSFNRVGLDHVVLVKVASTAVVTHMMGGTREEIVNAVSNAWIDGQSLRTYRHTPNTG
                     SRKSWAAGDATSRAVRLALMALKGEMGYPSALTAKTWGFYDVLFKGRPFRFKRPFGSY
                     VMENVLFKISYPAEFHAQTAVEAAIRLHPQVKARLDSVKKIVITTHESAIRIIDKKGP
                     LHNPADRDHCIQYMTAVGLIKGNLTAADYEDAVASDPRIDRLRSKMVCVENRQWSRDY
                     LDPGKRSIANAVQVFFNDGTKSEKAEVEYPVGHRRRRAEGVPLLEAKFRINLARRLSP
                     KQQAAILDLTRGQQRLEATPVHEFVDLFAA"
BASE COUNT         1101 a         1845 c         1682 g          905 t
ORIGIN      
        1 agaattccat gtcctggagc ggtatgcccg cgcgtgccgc catgccgagc ccgtcgccgg
       61 tgttgatgaa tgcgttggtg ctcgccgaaa aaatgcgccc ggcgccgccg gtggcgaaga
      121 ccgtggcctt cgcctgcagg atcatcactt cgcccgtttc catttcgagc gcggtgacgc
      181 cgaggacggc accgtcctgg ccgcgcacga ggtcaagcgc catccattcg acaaagaact
      241 gggttcgcgc gcggacattc gcctggtaca gggtgtgcag caatgcgtgt cccgtgcggt
      301 cggccgccgc gcacgcgcgg ggcaccgggg atttcccgta ttcgctcatg tggccgccga
      361 acggccgttg gtagatcttg ccgctcgcga gacggtcgaa cggcatgccg aagtgttcca
      421 gttcgtagac ggcctcgttc gccttgcggc acatgaactc gatcgcgtcc tggtcgccga
      481 gataatcgga gcctttgacc gtgtcgtaca tgtgccagtg ccagttgtcc tcgtcgatat
      541 tgccgagtga ggccgccacg ccgccctgcg ccgccaccgt gtgcgagcgc gtcgggaaaa
      601 ccttggacag gacggcgacc ttgaggtcgg cccgcgccag ctcgagcgcg gcgcgcatgc
      661 cggagccgcc gccgccgacg atcaccgcgt caaatgtcct aaccggcaat cccattcaca
      721 ggctccagag tatctgcacg gcccagatcc cgtaccagac cagcgcactg atcaccagca
      781 cgtagagcgt caggcgcagg gtcgggtcct tgatgtagtc catgaagatg ttgcgcacgc
      841 cgatccaggc atgcaggcac agggagagca gaaacagcac cgtcgcatac ttcacgatcg
      901 gtgtctgcca caccaccttc cactgccagt aatccatctt cggcaggccg gcgatcaccg
      961 acaggatcag cacggtgtag accgccatga tcacggcggt cacgcgctgt gccagccagt
     1021 cgcgcaggcc gtaatgggcg ccgacgacgt ctcgcttcac catatcatca ccgcgatcag
     1081 cagggtcagc acgatgccgc ccgccagcac tgcccagctg atcgcgcgcc cgctcgccag
     1141 gtccgtcccg taatggagat cgagcgcgag atggcgcagc cccgcgagca ggtggtgcag
     1201 gtacgcccag atcagtccga tcaggatcag cttgatggcc caatgcgaaa aagtgccctt
     1261 gaagcgcagg aacgtctgct gggactccag gctccactcg aacagcgtca gaaggaacgg
     1321 gatgaacagg aacagcgcgg cgccgctgac ccggtgcagg atcgatatca gccccggcac
     1381 cggcagccgg atctggaaca ggttgagata tttcggccgc ggcctagaca tcgagtttca
     1441 cggtctcgga caggggtttc tgaggggtcc tcacttgacg ctacccctgt gtcggtcttt
     1501 tgcttattga cgcttgaaat tctagcagtt ttcgctgcac cgcataagcc acgcgctggg
     1561 ctgctgtcgc ggccatccac gcggtggacg ttgctgcatc gcaccggcgt ggggcgttcg
     1621 gcttgtcacg cccatcgttg cttcgctatt atcatcggga gaacgagcac gcgcgaccgc
     1681 caccctgtcc cgtcgcacct ccttcccgaa ccgcaatatc actttccgca attcaggagc
     1741 ccaacagatg aaacgtccca tgcgcgttgc agtcaccggt gccgccggcc agatcggcta
     1801 cagcctcctg ccccggatag ccgccggaga gatgctcggg cgcgaccagc cggtcatcct
     1861 gcagttgctc gaaattcccg atgagaaggc gcaaaaggcg ttgaggggcg tgatgatgga
     1921 actcgacgac tgcgcctttc ccctgctcca cggcatggtg cccgccgcgg accccatggt
     1981 ggcattcaag gacgccgacg tggcgctgct ggtgggcgcg cgcccgcgcg gcccgggaat
     2041 ggaacgcaag gacctgctcg aagccaacgg caagatcttc gccccgcagg gacgggcgct
     2101 gtccgaagtc gccagccgga aagtcaaggt gctggtggtc ggcaaccccg ccaacaccaa
     2161 ttgcctgatc gcaatgaaga acgccccctg gctgaaaccc tcgaatttct cctcgatgat
     2221 gcgaattgac cacaatcggg cgctgaccca ggtcgcgcag aaaatcggcc tgcccgtcac
     2281 cgccatcagg aagatgacgg tgtggggcaa tcattcggcg acgcagtacc cagacctgtt
     2341 ccaggcgacc gccgggggca agaaagcctg gccgatgatc aacgaccagt catggctgga
     2401 gaacaccttc atcccggtca tccagaagcg cggcgccgcg atcatcgaag cacgtggatt
     2461 gtcttcggct gcaagcgcgg ccaacgcggc gatcgaccac gtccgcgact gggtcaaggg
     2521 caccaaggcc ggcgactgga ccagcatggg catcccttcc gacggcagct acggcatcgc
     2581 cgagggcgtc ctgtacggct atccggtcac ctgcaagaac ggccagtaca aaatcgtcaa
     2641 gggactggaa gtgagcgagt tcagccgcgg ccgcatgacg gcgacgctca aggagctgca
     2701 cgaagagcgg gattcgatca agcacctgct gggctaggca gggttcacga cgggggtgcg
     2761 cgggcagtaa cgctcgcaca cccctgttct cttaacgctg gagcacgccc atgagtgaag
     2821 cgcaagccgg cgccaagccg aagaagtcgg tagcactgtc gggcattgcg gccggcagca
     2881 ctgcgctttg caccgtcggc cgcagcggca acgatctcca ttaccgcggc tacgacatcc
     2941 tcgatatcgc cgatcaatgc gagttcgagg agattgccca cttgctggtg cacgagaaac
     3001 tccctacccg ggcggaactc tcgtcataca agaagaaact cgggtccctg cgcggactac
     3061 ccgccccggt gaaaaccacg ctcgagcaga ttcccgcctc ggctcacccg atggacgtgc
     3121 tgcgcaccgg ctgctcggcg ctcggcgcgg tggcgccgga gaaggacgat cacggcatcg
     3181 cgggcgcgcg cgatatcgcg gaccgcctga tggcctcgtt cggctcgatg ctgctctact
     3241 ggcaccacta cagccgcgac aggcgccgca tcgaggtcga aaccgatgac gactccatcg
     3301 gcgggcattt cctcacgctc ctgcacggca ggccatcgaa accgctgtgg atcaagtcca
     3361 tgcacacttc gttgaatctc tacgccgagc acgaattcaa cgcctccacc ttcgccgccc
     3421 gcgtcgtggc cggaaccggg tccgatatgc attccgcagt cactggcgcg atcgggacgc
     3481 tgaagggtcc caagcacggc ggcgccaacg aagtggggtt cgaagtgatg aaccgctacg
     3541 agagccctga tgccgccgaa cgcgacatcg tagagcgcat ccgcaacaag gaggtcgtga
     3601 tcggcttcgg ccatccggtc tacaccatcg ccgacccgcg caacaagatc atcaaggaag
     3661 tcgcgcgcag gctctcgcag gatgccggcg agatggagat gttcgaaatc gccgagcgta
     3721 tcgagcaggt catgatgcgc gagaagaaga tgttcgccaa tctcgactgg ttctccgcgg
     3781 tctcctacca ccggatgggc gttcccacgg caatgttcac gccgattttc gtcatcgccc
     3841 gcaccagcgg ctgggcggcg cacgtgatcg agcagcgcat cgacaacaag atcatccgtc
     3901 ccaccgcggt ctacacggga cccgagccgc gcaaattcat ccccatggat caacgcaaat
     3961 gagcagtcac atctccaacg tccgcgccgc gcccgataaa gtcctcaccg acatcaccga
     4021 ttacgttgcc ggccgcgcca tcaacagcaa cgaggcctac aacacggcgc ggctgtgcct
     4081 gatggacacg ctcggctgcg gactggaagc gctcgaatac cccgcctgca ccaagctgct
     4141 gggcccgatc gtgcccggcg ccgtgctgcc gaacggcgcg aaagtgcccg gcacgccgca
     4201 ccagctcgac ccggtcaccg cggccttcaa catcggcgcc atgatccgct ggctcgattt
     4261 caatgacacc tggcttgcgg ccgaatgggg ccatccatcc gacaacctcg gcggcatcct
     4321 cgccaccgcc gactggctgt cgcgcacccg cctcgcccgg ggcggcaagc cgctcctcat
     4381 gcgcgacgtg ctgaccggca tgatcaaggc ccacgagatc cagggcgtga tcgcgctcga
     4441 gaacagcttc aaccgcgtcg gcctcgacca tgtcgtcctg gtcaaggtcg cctccaccgc
     4501 cgtcgtcacc cacatgatgg gcggtacccg cgaggaaatc gtgaatgcgg tctccaatgc
     4561 ctggatcgac gggcaatcgc tgcgcaccta ccgccacacg cccaacaccg gctcgcgcaa
     4621 gtcatgggcg gcgggcgatg ccaccagccg ggcggtgcgg cttgccctga tggcgctgaa
     4681 gggcgagatg ggctatccct ccgccctgac cgcgaagacc tggggcttct acgatgtttt
     4741 gttcaaaggc aggccattcc ggttcaagcg gcctttcggc tcgtacgtga tggagaacgt
     4801 gctgttcaag atttcctacc cggccgagtt tcacgcccag accgccgtgg aggcggccat
     4861 ccggctgcac ccgcaggtga aggcccggct cgacagcgtg aagaagatcg tcatcacgac
     4921 ccacgaatcc gcgatccgca tcatcgacaa gaaaggcccc ctgcataacc cggcggaccg
     4981 cgaccactgc atccagtaca tgaccgcggt cgggctcatc aaaggcaact tgaccgccgc
     5041 cgactacgag gacgcagtcg catccgatcc acgcatcgac cgcttgcggt cgaaaatggt
     5101 ttgcgtcgaa aacaggcagt ggagccgcga ttacctggac cccgggaaac gctccatcgc
     5161 caatgcggtt caggttttct tcaatgacgg gacgaaaagc gaaaaggccg aagtggaata
     5221 tcccgtcggc catcgccgcc gccgcgccga aggcgtgccg ctgctggagg ccaagttcag
     5281 gatcaacctt gcgcgccggc tttcgcccaa gcagcaggcg gccatcctgg acctgactcg
     5341 cggccagcag cgcctggagg caacgccggt ccacgaattc gtggatctct tcgcggcgtg
     5401 acgaggagat aagagtccct aacataatga aacgcgcgcg gcgctgtcac gattttggct
     5461 cagggcgcga agcgagccgc agcccatata ggccatattg gcaaggcgag cgacaaagcc
     5521 atgggccaaa act
//
LOCUS       DBNGSG010000146         3357 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1578338, whole genome shotgun sequence.
ACCESSION   DBNGSG010000146 DBNGSG010000000
VERSION     DBNGSG010000146.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1578338"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..484)
                     /locus_tag="AC158Q_03835"
     CDS             complement(20..484)
                     /locus_tag="AC158Q_03835"
                     /inference="COORDINATES: protein motif:HMM:NF016221.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HIR3338931.1"
                     /translation="MKWIFIAIHIAAGVLAVAPATAHGYGKGELQVRHPWARATPPGA
                     KVAAGYLELRNSGNEPDRVVGASTPAAERVELHVLSREGDVMKMREVKGFEVPARQRL
                     VLRPGGSHLMIVGLKRPFVKGERVPLTLRLERAGELHIELEVQPADSGKPHH"
     gene            complement(547..1266)
                     /locus_tag="AC158Q_03840"
     CDS             complement(547..1266)
                     /locus_tag="AC158Q_03840"
                     /inference="COORDINATES: protein motif:HMM:NF015506.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR3338932.1"
                     /translation="MLHDHYGTTGKREPDPRWRPTRALLIFGLASFAVHAAVIVALPD
                     FFQGPGPANVSMLEVTVLKIEPLPVAEAQPESIPSRLPSEPKSRPTHTPLKTERKQSP
                     PVLALSKSEPAPEDSFAVAPARLPEPSQPASGTKPQAASAAVVPPSVNAAYLSNPAPR
                     YPLASRRAGEQGTVTLRVLVKPDGYPAQVEIERTSGSPHLDAAALEAVRGWRFVPARQ
                     GANPVESRVLVPVVFRLEGAS"
     gene            complement(1336..1854)
                     /locus_tag="AC158Q_03845"
     CDS             complement(1336..1854)
                     /locus_tag="AC158Q_03845"
                     /inference="COORDINATES: protein motif:HMM:NF023164.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3299 domain-containing protein"
                     /protein_id="HIR3338933.1"
                     /translation="MRNLVWFIALLAGFPTAVWAQQKDVPGKDLYAKDPARMDAPVAV
                     PPQYLKPLPELKGVVSWKTLADVKPVKQKDRFVPQFSKEISALDRKDVKLQGFMMPLD
                     MGEKQKRFLLVALPPSCAFCLPGGPDQLVEVVAKTPVKYGFDPIVISGKFVVLKDDPM
                     GLYYRLTDAVAQ"
     gene            complement(1908..2171)
                     /locus_tag="AC158Q_03850"
     CDS             complement(1908..2171)
                     /locus_tag="AC158Q_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338934.1"
                     /translation="MKEWIAAALLAGFAMMPGATLAAEKAGKDDPAHVSKDVQDHRAM
                     AEAHLNAARCLESGRTEKECHAQLAKDCKGLGIGKYCGMKHRH"
     gene            complement(2242..>3357)
                     /locus_tag="AC158Q_03855"
     CDS             complement(2242..>3357)
                     /locus_tag="AC158Q_03855"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3338935.1"
                     /translation="LGVATITLLLLTLHQLEERMGRDARGIDLVAGAKGSPMQVILSA
                     IYHLDVPTGNIPLAQAKEIAKHRMVKKTIPLALGDSYKGFRIVGTNHDYVAHYGGSTA
                     AGRLWEKPMEAVIGVEVAQRLKLGVGGTFTGAHGIADAGAEHGDAPYRVVGVLAASGS
                     VLDRLVLTSIESVWLVHDEHMGTDAKMTEEDKEITALLIQYATPLAAAMMPRYINQNS
                     EMQAASPAYETARLFSVIGVGVDVLRGFALVLILSAGLSLFIALTNALAERRYDLALM
                     RALGASPAKLMALMLFEGVLLAGLGAVLGLAAGHALTEALGYTLKAAQQVAVTGRVWL
                     IEELWLVGIALAVGIVAALLPAWRAYRTDIAHTLARG"
BASE COUNT          553 a         1155 c         1061 g          588 t
ORIGIN      
        1 ttcgcctttc gacttataac tagtggtgcg gctttcccga atcggcaggc tgaacctcca
       61 gctcgatatg cagttcaccc gcccgctcga ggcgcagcgt gagcgggacg cgctcgcctt
      121 tgacgaacgg tctcttgagg ccgacgatca tcaggtgcga tccgcccgga cgcagaacca
      181 gccgttggcg cgccggcacc tcgaagccct tcacttcgcg catcttcatg acgtcgccct
      241 cgcgcgacag gacgtgcagc tcgacgcgct cggcggccgg tgtcgaggcg ccgacgacac
      301 ggtccggctc gttccctgaa ttgcgcaatt ccaggtaacc cgccgcgacc ttcgcgcccg
      361 gcggcgtagc acgggcccag ggatggcgta cctgaagctc gcctttgccg tagccatgcg
      421 ccgtcgccgg cgcgaccgcc agcacgccag cggcaatgtg gatcgcaatg aatatccatt
      481 tcacaaagtg tcgccagttc acacagggtt cctgaatctc aagcagtgga ttcgcgattc
      541 acgcttctac gaagcgcctt cgagccggaa cacgaccggc accagcaccc gggattcgac
      601 cgggttcgcg ccctgccgcg ccggcacgaa gcgccagccc ctgaccgctt ccagcgccgc
      661 ggcatcgaga tgcggcgagc cgcttgttcg ctcgatctcg acttgcgccg gatatccgtc
      721 cggtttcacc agcaccctca gcgtgaccgt gccctgctcg ccggcgcgcc gggaggccag
      781 cggatagcgc ggcgcgggat tgctgagata ggcggcgttg acgctggggg gcaccaccgc
      841 ggcgctcgcc gcctggggct tcgtgccaga agcgggctgg gacggctcag gaagcctcgc
      901 tggcgctact gcgaacgagt cctccggcgc cggttccgat ttcgacaagg cgaggaccgg
      961 cggcgattgc ttgcgctccg tcttcaatgg cgtatgcgtt ggcctgcttt tcggttcgga
     1021 aggcagccgc gaagggatgg actccggttg ggcctccgcg accggcagcg gctcgatttt
     1081 caggacggtc acctccagca tgctgacatt ggccgggccg gggccctgga agaagtccgg
     1141 cagcgcaaca atgaccgccg catgcacggc aaagctcgcc agcccaaata tcagcagcgc
     1201 tcgcgtggga cgccagcgcg gatccggctc gcgtttcccg gtcgtgccat agtgatcgtg
     1261 aagcattcag gagtcgcgca cccccgattc aggggccgcg gtatcggaag cgggcggaaa
     1321 accgggcgaa ggcggctatt gcgcgaccgc gtcagtcagg cggtagtaaa gccccatcgg
     1381 gtcgtctttg agcacgacga acttgccaga aatgacgatg ggatcgaacc cgtacttgac
     1441 cggagtcttg gccaccacct cgaccagctg gtcgggcccg cccggcaggc agaacgcgca
     1501 cgacggcggc agcgcgacga gcaggaagcg cttctgcttt tcccccatgt cgagcggcat
     1561 catgaagccc tgcagcttca cgtctttcct gtccagcgcg gagatttcct tcgagaactg
     1621 cggaacgaac cggtcctttt gcttgaccgg cttgacgtcg gcaagcgtct tccaggaaac
     1681 gacgcccttc aactcgggca gcggcttgag atactgcggt ggcaccgcaa cgggcgcgtc
     1741 catccgcgcc ggatccttgg catagagatc cttgccggga acgtcctttt gctgcgccca
     1801 tacggccgtg gggaagcccg ccagcaacgc gatgaaccaa acaaggtttc tcatggctac
     1861 ctccatccga tactgcgccg attttactcc catcgccccg gggaacatca atgccggtgc
     1921 ttcatcccgc agtacttgcc tatccccagc cccttgcagt ccttggcaag ctgggcgtgg
     1981 cattccttct ccgtccggcc ggactccagg caccgggcgg cgttgaggtg cgcttccgcc
     2041 atggcgcggt gatcctggac gtccttcgac acatgagcgg gatcgtcctt cccggctttc
     2101 tccgccgcga gcgtggcacc gggcatcatc gcgaacccgg cgagcagagc cgctgcgatc
     2161 cattctttca tggcttgtcc tcgatcaggc aattgcaaat agcgtttgtt ccaccagcat
     2221 agcctgcctg tcaagttctg gtcatccccg agcgagggta tgcgcgatat cggtgcggta
     2281 ggcgcgccac gccggcagca gcgcggcgac gatgcccacc gcgagcgcga tgccgaccag
     2341 ccacagttcc tcgatgagcc agacccgtcc ggtcaccgcc acctgttgcg cggctttcag
     2401 cgtgtagcca agggcctcgg tcaacgcgtg gcccgccgcg aggccaagca ccgcgcccag
     2461 ccccgcgagg agcacgccct cgaacagcat cagcgccatc agttttgccg ggctcgcgcc
     2521 gagcgcgcgc atcagcgcga gatcgtagcg gcgctcggcg agcgcgttgg tcagcgcgat
     2581 gaacagggac aggcccgccg acaggatcag caccagcgcg aagccccgca gcacgtccac
     2641 gcccaccccg atcacgctga acaggcgcgc ggtctcgtag gcgggtgacg cggcctgcat
     2701 ctcgctgttc tggttgatgt agcgcggcat catcgcagcg gcgagcggtg tcgcgtactg
     2761 gatcagcagc gcggtgatct ccttgtcctc ctccgtcatc ttcgcgtcgg tccccatgtg
     2821 ctcgtcgtgc accagccaga cgctctcgat gctggtcagc accagccggt cgagcaccga
     2881 tccgctcgcc gccagcacgc cgaccacccg gtacggggca tcgccatgct cggcgccggc
     2941 gtcggcaatg ccgtgagcgc cggtgaacgt gcccccgacg cccagcttca ggcgttgcgc
     3001 cacctcgacg ccgatcaccg cttccatcgg cttctcccac agccggcccg cggcggtgct
     3061 gccgccgtag tgcgcgacgt aatcgtgatt cgtgccgacg atgcggaagc ctttgtagct
     3121 gtctccgagc gcgagcggaa tggttttctt caccatgcgg tgcttggcga tttccttcgc
     3181 ctgcgcgagc gggatgtttc cggtcgggac atcgaggtgg tagatcgccg acaggatcac
     3241 ctgcatcggg ctgcccttgg cgccggcgac gaggtcgatg ccgcgcgcat cacgccccat
     3301 gcgctcctcg agctggtgca gagtgagcag cagcagcgtg atggtcgcga ccccgag
//
LOCUS       DBNGSG010000147         1857 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2936581, whole genome shotgun sequence.
ACCESSION   DBNGSG010000147 DBNGSG010000000
VERSION     DBNGSG010000147.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2936581"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="AC158Q_03860"
     CDS             <1..617
                     /locus_tag="AC158Q_03860"
                     /inference="COORDINATES: protein motif:HMM:NF014724.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HIR3338936.1"
                     /translation="YALSTPFLADELLGSLEPAPRDPLADRSGQAIIVLGGGTYFSAP
                     EYGRDTVRASTLLRLRYAAHLQRTLNKPVMVTGGAPRGNAGSEAEQMQQVLQHDFRVP
                     VQWTERSSNNTLENARLSYRLLDAAGLRRVYLVTHAWHMRRARLAFERAGFAVIPAPT
                     GYTTRHKLTALDFLPDADALYRSNRFFREALGLGWYHLRFLSGH"
     gene            636..1058
                     /locus_tag="AC158Q_03865"
     CDS             636..1058
                     /locus_tag="AC158Q_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR3338937.1"
                     /translation="MKVRIKWLENVAFVGESESGHALVMDGAPEGGGRNLGPRPMETV
                     LIGTGGCTAYDVVHILRKGRAPVTDCVVEIAAERADQDPKVFTRIHFHFVVTGKSLKR
                     EQVERAIHLSAEKYCSASIMLGKMAAITHDFEIREAGN"
     gene            complement(1277..>1857)
                     /gene="coq7"
                     /locus_tag="AC158Q_03870"
     CDS             complement(1277..>1857)
                     /gene="coq7"
                     /locus_tag="AC158Q_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HIR3338938.1"
                     /translation="TVFAPARSLRPTPGRDLVEAEMTDQQRRLSASLMRVNHTGEICA
                     QALYQGQALTARNPRAKAALEKAAQEETEHLAWTERRIEELGGRKSLLNPAWYAGSFA
                     LGALAGIAGDRWNLGFLAETERQVVTHLKGHLQRLPEQDARSRAIVESMKEDEARHAT
                     SAISHGGAELPEPAKAAMRLSSKVMTETAFWL"
BASE COUNT          316 a          617 c          573 g          351 t
ORIGIN      
        1 tctacgcgct ttccacccct ttcttggcgg atgaactgct cgggtcgctg gaaccggcgc
       61 cacgcgaccc cctggctgac cggagcggcc aggccatcat cgtgctgggc gggggcacct
      121 atttctcggc gccggagtac ggccgggata cggtcagggc gagcactctc ttgcgattgc
      181 gttacgccgc gcacctgcag cggacattga acaagccggt catggtcacc ggcggcgcgc
      241 cacgaggcaa cgccggctcc gaggcggagc agatgcaaca ggtgctccag cacgatttcc
      301 gcgtcccggt tcagtggacc gagcggagct cgaacaacac cctggagaac gcccggctga
      361 gctaccgcct gctcgatgct gcgggcctgc gtcgcgtcta ccttgtcacc cacgcctggc
      421 acatgcggcg cgcgcggctg gctttcgagc gcgccggctt tgcggtcatc cccgccccga
      481 ccggctacac cacgcgccac aagctgaccg ctctcgattt cctcccggac gcggatgcgc
      541 tctaccgcag caaccgcttt ttccgggagg ccctcggcct cggctggtat catctgcgct
      601 tcctgtcggg gcactaaccg gatgacagtc cgcgaatgaa agtccgcatc aagtggctgg
      661 aaaacgtcgc ttttgtcggt gagtccgaaa gcgggcacgc gctcgtcatg gacggcgccc
      721 cggaaggcgg cgggcgcaac ctgggcccgc gcccgatgga gacggtgctg atcggcaccg
      781 gcggctgcac cgcgtacgat gtcgtgcata tcctcaggaa gggccgcgcc ccggtcaccg
      841 attgcgtggt ggagatagcc gcggagcgcg ccgatcagga tcccaaggtc ttcacgcgaa
      901 tccacttcca cttcgtcgtc acggggaaaa gcctgaaacg cgagcaagtc gagcgcgcca
      961 tccatctctc agccgagaaa tactgctcgg cctcgatcat gctcggcaaa atggccgcga
     1021 tcacccacga cttcgagatt cgagaagcag gaaattaacc gccacgacgc caaggccgcc
     1081 aagaggatca aaattaaaga aaaactagaa caaggacgga aattggccgc ttcagggttt
     1141 ttcgtggctg cctatattat cgctggcgct aatccatcaa cgcgagatta cgtagttccg
     1201 ttcttattgt tatcttgttt ttcttggcgg ccttggcgtc ttggcggttc gttctgaatt
     1261 ctgatctgct tggcggtcag agccagaacg cggtttcggt catcaccttc gaagacaggc
     1321 gcatcgcggc tttcgcgggt tccggcagtt ccgccccgcc gtgactgatg gcgctcgtgg
     1381 cgtgccgcgc ctcgtcttct ttcatgctct cgacgatcgc gcggctcctg gcatcctgct
     1441 ccggcaggcg ctgaagatgt cccttcagat gcgtgaccac ctggcgctcg gtctccgcga
     1501 gaaaacccag gttccagcgg tcgccggcaa tcccggcgag cgcgccgagc gcgaacgagc
     1561 cggcatacca cgccgggttg agcaggctct tgcggccgcc cagttcctcg atgcgtcgct
     1621 cggtccaggc gaggtgctcg gtctcttcct gcgccgcctt ttccaacgcc gctttggcac
     1681 gtggattgcg ggccgtcaac gcctgcccct ggtagagcgc ctgcgcgcaa atctcgccgg
     1741 tgtgattgac ccgcatcagc gatgcggaaa ggcggcgctg ctggtccgtc atttcagcct
     1801 cgacaagatc ccggcccggc gttgggcgca gcgaccgcgc cggcgcaaat accgtgc
//
LOCUS       DBNGSG010000148         2812 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_389969, whole genome shotgun sequence.
ACCESSION   DBNGSG010000148 DBNGSG010000000
VERSION     DBNGSG010000148.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_389969"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..658
                     /gene="folK"
                     /locus_tag="AC158Q_03875"
     CDS             143..658
                     /gene="folK"
                     /locus_tag="AC158Q_03875"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HIR3338939.1"
                     /translation="MAPVTRHPSLVTAFVALGGNIGDPPAQIRSALRALAKLRETRLV
                     RRSSLYRNPPVGHLDQPEFVNAVAQIETRIGPRELLGHLLEIERKHGRERGYPNAPRT
                     LDLDILLYGGQVVHEPGLVIPHPRMPERAFVLVPLAEIAPDIEVPGRGRVAGLLRNVD
                     ASGMIRLTETE"
     gene            859..1509
                     /locus_tag="AC158Q_03880"
     CDS             859..1509
                     /locus_tag="AC158Q_03880"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HIR3338940.1"
                     /translation="MTAKLPDKYRYIVVEGPIGAGKTSLARVMSRRTGGEALLEDPDA
                     NPFLPGFYQDRARYAMPTQLYFLFQRATQLRALSQPDLFSGVTVADFMIEKDALFARL
                     TLSDDEFALYRQVYAQLKPQAPAPDLVVYLQASPETLIDRVRRRGVAYERDIPDEYLV
                     RLVELYARFFYQYDAAPVLTVNSDNLNFVDQPGDFDLLLSRIAGMRGPREFFSLGN"
     gene            1517..2308
                     /gene="panB"
                     /locus_tag="AC158Q_03885"
     CDS             1517..2308
                     /gene="panB"
                     /locus_tag="AC158Q_03885"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HIR3338941.1"
                     /translation="MRITLTTLQRMAQEGSKIAMLTCYDASFAAVLEEAGVDSLLIGD
                     SLGNVLQGHESTLPVTLRDMVYHTGCVARGSKRAFIVADMSFGSFQVSPEETMRNAAE
                     LMAAGSQMVKIEGGRPMLETIEFLTDRGVPVCGHLGLTPQSVHQLGGYRVQGKGESEG
                     QRLLREAKHLEQAGAGMIVLEAIPAALAREVTAALTIPTIGIGAGVDCSGQVLVLHDM
                     LDVYPGKKAKFVKNYMRPTGSIQGAAELYVKEVKARTFPGPEHSF"
     gene            2391..>2812
                     /locus_tag="AC158Q_03890"
     CDS             2391..>2812
                     /locus_tag="AC158Q_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HIR3338942.1"
                     /translation="MDIIHSAAALRERLQREPNNVFVPTMGTLHDGHIQLIDIAKPRA
                     ACTVVSIFVNRLQFGPREDFDRYPRTLDADSARLKAAGVDVLFLPEEKEIYPERQTFV
                     VEPSDLQYILEGAHRPGHFRGVATVVLKLFNLVMPHSA"
BASE COUNT          486 a          922 c          912 g          492 t
ORIGIN      
        1 gccggcgaag ccgagcgcgc ccagatgctg gtcgcggaca gcgagccccg cgggcgtcgc
       61 cgccggcgcc gtcggaaacc gcgcggcccc ggtgcggatc cggggccagt cggggataca
      121 agtcccgcag ggtagcggag ccttggcacc cgtcacccgt cacccgtcac tcgtcaccgc
      181 gttcgtggca ttgggcggca acatcggcga cccgccggcg cagatccgct ccgcgctgcg
      241 cgcgctggcg aagttgcgcg aaacacgcct cgtgcgccgg tcttctctct atcgcaaccc
      301 gccggtgggg catctcgacc agccggaatt cgtgaacgcg gtggcgcaaa tcgagacccg
      361 catcggcccg cgcgagctgc tcgggcatct gctcgagatc gagcgcaagc atggccgcga
      421 gcgcggctac cccaacgcgc cgcgcacgct cgatctcgat atcctgctct atggcgggca
      481 ggtggtgcac gagccggggc ttgtcatccc gcatccgcgc atgccggaac gcgcttttgt
      541 cctggttccg ctggcggaaa tcgcgccgga tatcgaggtg ccgggcaggg ggcgcgtcgc
      601 cggcctgctg cggaacgtgg atgcgtccgg catgatcagg ctgactgaaa ccgaatagac
      661 aggatcaacg agaaaagatc gagaatccgt tgttcgcttt aatggacagg attaacaaca
      721 ttaacaagaa tggaacccag gccgtgaaac gttgctcgag ttcgagtttg ttgaatccta
      781 cagatcctct tgatcctggc atttcatggt tccaggttcc tggcttgttt atcctgttaa
      841 tcctgtctat ttcttgacgt gacagcgaag ctgcccgaca agtaccgcta catcgtcgtc
      901 gagggcccga tcggcgcggg caagacgagc ctggcgcgcg tgatgagccg gcgcacgggc
      961 ggcgaggcgc tgctggagga tccggatgcc aacccgttcc tgcccggctt ctaccaggac
     1021 cgcgcgcgct acgcgatgcc gacgcagctc tatttcctgt tccagcgcgc gacgcagctg
     1081 cgcgcgctgt cccagcccga cctgttctcc ggcgttaccg tcgccgactt catgatcgag
     1141 aaggacgccc tgttcgcgcg gctcacgctg agcgacgacg agttcgcgct ctaccggcag
     1201 gtctatgcgc aactcaagcc gcaggcgccg gcgcccgatc tcgtcgtcta cctccaggcc
     1261 tcgcccgaga cgctgatcga ccgggtgcgc cgccgcggcg tcgcctacga gcgcgacatt
     1321 ccggacgagt atctggtccg gctggtggag ctttatgcgc ggttcttcta ccagtacgac
     1381 gcggcgccgg tgctgaccgt caattccgac aatcttaatt ttgttgatca gcccggtgat
     1441 tttgatttat tgttgagccg cattgcgggc atgcgcggcc cgcgcgaatt ctttagcctc
     1501 ggaaactagg acggtcatgc ggatcaccct caccaccctg cagcggatgg cgcaagaagg
     1561 cagcaagatc gcgatgctga cctgctacga cgcgagcttc gccgccgtgc tcgaggaggc
     1621 gggcgtcgac tcgctgctga tcggggattc gctcggcaac gtcctgcagg ggcacgagtc
     1681 cacgttgccg gtcacgctgc gcgacatggt ctaccacacc gggtgcgtcg cgcgcggctc
     1741 gaagcgcgcc ttcatcgtcg ccgacatgtc gttcggcagc ttccaggtgt cgccggagga
     1801 gaccatgcgc aacgcggctg aactgatggc cgccggatcc cagatggtga agatcgaggg
     1861 cggcaggccg atgctggaga cgatcgaatt tctcaccgac cgcggcgtgc cggtatgcgg
     1921 gcacctgggg ctcacgccac aatcggtgca ccagctgggc ggctaccgcg tgcagggcaa
     1981 gggcgagagc gagggccagc gcctgctgcg ggaagccaag cacctcgagc aggccggggc
     2041 gggcatgatc gtgctcgagg cgatacccgc ggcgctggcg cgcgaggtca ccgcggcgct
     2101 cacgataccg accatcggca tcggcgccgg cgtggattgc tcggggcagg tgctggtgct
     2161 gcacgacatg ctcgacgtct atcccggcaa gaaggcgaaa ttcgtcaaga attacatgcg
     2221 tcccacggga tcgatccagg gcgcggccga gctctacgtc aaggaagtca aggccaggac
     2281 cttccccggc cccgagcact ccttctaatg atgagtgcaa agtgcagagc gcgaagtgaa
     2341 ggagcgcttt ccgctcatcg ctcatcgctc atcgctcatc actgaacttc gtggacatca
     2401 tccattccgc tgccgcgctc cgggagcgcc tgcagcgcga gcccaacaac gtgttcgtgc
     2461 ccaccatggg caccctgcac gacgggcaca tccagctgat cgacatcgcc aagccgcgcg
     2521 cggcctgcac cgtggtgagc atattcgtga accgcctgca gttcggtccg cgcgaggact
     2581 tcgaccgcta cccgcgtacg ctggatgccg acagcgcgcg cctgaaagcg gcgggcgtgg
     2641 acgtcctgtt cttgccggag gagaaggaga tctacccgga gcgccagacc ttcgttgtcg
     2701 aaccctccga cctgcagtac atcctggagg gcgcgcaccg cccgggtcac ttccgcgggg
     2761 tcgcgaccgt ggtgctgaag ctcttcaacc tggtgatgcc gcactcggcg at
//
LOCUS       DBNGSG010000149         2300 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_390296, whole genome shotgun sequence.
ACCESSION   DBNGSG010000149 DBNGSG010000000
VERSION     DBNGSG010000149.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_390296"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="AC158Q_03895"
     CDS             <1..405
                     /locus_tag="AC158Q_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3338943.1"
                     /translation="ERGFSVWRRFGGSTDVLESVGLAGKAHVRAKDLSYGDRRALEIG
                     VALAARPRLLFLDEPTAGLGTEATARIARLIAELKRSITIVLIEHDMKFLFGLADHIY
                     VMHWGQVIAQGSPAELRANPWVQRSNLGHAVN"
     gene            392..1087
                     /locus_tag="AC158Q_03900"
     CDS             392..1087
                     /locus_tag="AC158Q_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3338944.1"
                     /translation="MLSISDIHAFYGETQVIFGVSIDVAQGEVVALLGPNGAGKTTTL
                     RSILNLASVRSGRISFDGRNITQQPTHEIARAGIGWVPDDRRVFPTLTVEKNLAIAKK
                     RTRFRAWSERECFEIFTALEYLRWRECENLSGGEMQMVAISRALIGSPGLVLFDEPSQ
                     GLAPKVVHDVLRVIRRLKDERVAVLLVEQNPLAALGVSDRVYVMDKGRIVYHGEAAAL
                     RRDGALRQRLVGV"
     gene            1087..1677
                     /locus_tag="AC158Q_03905"
     CDS             1087..1677
                     /locus_tag="AC158Q_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3338945.1"
                     /translation="MLLAVENLVKEYKRRWRTTFRLEASFSVERPEIIGVMGPNGSGK
                     TTLFELITGSNTPSAGRVVCAGQDIHRVKTGERDRLAIHYHQSYQVRRFRRYKPDFLL
                     EPARSEYPLVHLFDEPQFNTQDGYIGFMLDFFRRLRGEGRVVFVCLHPNESYHLDILR
                     EVCERFIFVERGRIALHAGWNALVADARVAGYLRTT"
     gene            1674..2048
                     /locus_tag="AC158Q_03910"
     CDS             1674..2048
                     /locus_tag="AC158Q_03910"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3338946.1"
                     /translation="MKQKFAFSRGAEARYKPGFRPYFQDRDLGVKQATGGRFTAEVHR
                     ACAPCPKEGSGVHSHSVDFQFNYVLKGWIRVWIDGEGEFTFSAGDTWIQPPAIRHDVR
                     GFSDDFEVLEIVSPGIFGTHEA"
     gene            2060..>2300
                     /locus_tag="AC158Q_03915"
     CDS             2060..>2300
                     /locus_tag="AC158Q_03915"
                     /inference="COORDINATES: protein motif:HMM:NF012398.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3338947.1"
                     /translation="MNKPAEMKLETVPAWIGGKPVSSAQRFGDVFNPATGQITKRVAI
                     ADSKSIGSAVSAAAAAFPQWRDTPPVRRARIMQEFL"
BASE COUNT          383 a          762 c          765 g          390 t
ORIGIN      
        1 gagcgcggat tcagcgtgtg gcgccgcttc ggcggcagca ccgatgtcct ggaaagcgtc
       61 ggcctcgccg gcaaggcgca cgtgcgcgcg aaggacctgt cctacggcga ccggcgcgcg
      121 ctcgaaatcg gcgtggcgct cgccgcgcgg ccgcgcctgc tctttctcga tgagccgacc
      181 gccggccttg gcaccgaggc gacggcacgc atcgcgcgcc tcatcgccga gctcaagcgc
      241 tcgatcacca tcgtgctgat cgagcacgac atgaagttcc tgttcggcct cgccgaccac
      301 atctacgtca tgcactgggg ccaggtgatc gcgcaaggca gcccggccga gctgcgcgcc
      361 aacccctggg tccagcgctc caatctcggc catgctgtca attagcgaca ttcatgcgtt
      421 ctacggcgag acgcaggtga tcttcggcgt ctcgatcgac gtggcgcagg gcgaggtggt
      481 cgcgctgctg ggccctaacg gtgctggcaa gaccacgaca ttgcgatcaa tcctgaacct
      541 cgcttcggtt cgatcgggcc gcatctcctt cgatggaagg aacattacgc agcagccaac
      601 gcacgagatc gcgcgcgccg ggatcggctg ggtgcccgac gaccggcgcg tgttcccgac
      661 ccttacggtg gagaaaaacc tcgccatcgc gaagaagcgc acgcgctttc gcgcctggag
      721 cgagcgcgag tgcttcgaga tcttcaccgc gctcgaatac ctgcgctggc gcgaatgcga
      781 gaacctgtcg ggtggcgaga tgcagatggt ggcgatctcg cgcgcgctga tcggctcgcc
      841 gggcctcgtg ttgttcgacg agccgagcca ggggctcgcg cccaaagtgg tgcacgacgt
      901 gctgcgcgtg atccgcaggc tgaaagacga gcgcgtcgcg gttctgctcg tcgagcagaa
      961 cccgctcgcg gcgctcggcg tctccgaccg cgtttacgtc atggacaagg ggcgcatcgt
     1021 gtaccacggc gaggcggcgg cgctgcggcg cgacggcgcg ctgcggcaga ggctggtcgg
     1081 ggtctgatgc tgctcgccgt cgaaaacctg gtcaaggaat acaagcggcg ctggcgcacc
     1141 acgttccggc tcgaggcaag cttctccgtg gagcggccgg agatcatcgg cgtgatgggt
     1201 ccgaacggct cgggcaagac gacgctgttt gagctcatca cgggctccaa caccccgagc
     1261 gccgggcgcg tggtgtgcgc cggccaggac atccaccgag tgaagaccgg cgagcgggac
     1321 cgcctggcca ttcactacca ccagtcctac caggtgcgcc gcttccggcg ctacaagccc
     1381 gatttcctgc tcgagccggc gcgctcggaa tatccgctcg tgcatctctt cgacgagccg
     1441 cagttcaaca cccaggatgg ctacatcggg ttcatgctcg atttttttcg ccgcctgcgc
     1501 ggcgaggggc gcgtggtgtt cgtctgcctg cacccgaacg agagctacca tctcgacatc
     1561 ctgcgcgagg tatgcgaaag gttcatcttc gtcgagcgcg gacgcatcgc gctgcacgcg
     1621 ggctggaacg cgctcgtcgc cgatgcgcgc gtggcgggct atctgaggac gacatgaaac
     1681 agaaattcgc tttcagccgc ggcgccgagg cgcgctacaa gcccggtttc cgtccctact
     1741 tccaggaccg cgacctcggc gtgaagcagg ccaccggcgg acgctttacc gcggaagtcc
     1801 atcgcgcctg cgccccctgc cccaaggagg gatccggcgt gcactcgcac tcggtcgact
     1861 tccagttcaa ctatgtcctg aaaggctgga tccgcgtgtg gatcgacggc gagggagagt
     1921 tcaccttcag cgccggggac acctggatcc agccgcccgc catccgccac gacgtgcgcg
     1981 gcttctccga cgatttcgag gtgctggaga tcgtttcccc ggggattttc ggaacgcacg
     2041 aggcttagaa tcccgggcta tgaacaagcc cgcagagatg aagctggaaa ccgtcccggc
     2101 gtggatcggc ggcaagccgg tctcgtccgc gcagcgcttc ggcgatgttt tcaacccggc
     2161 cacgggtcag atcacgaagc gcgtggcgat tgccgattcc aagagcatcg gttccgcggt
     2221 gagcgcggcc gcggccgctt tcccgcaatg gcgcgacacg ccgccggtgc ggcgggcgcg
     2281 catcatgcag gagttcttgt
//
LOCUS       DBNGSG010000150         4387 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2427797, whole genome shotgun sequence.
ACCESSION   DBNGSG010000150 DBNGSG010000000
VERSION     DBNGSG010000150.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4387)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2427797"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="AC158Q_03920"
     CDS             complement(<1..432)
                     /locus_tag="AC158Q_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338948.1"
                     /translation="MQVAKHKPIVAPFAWKGADLQDRTDWIRPFAPAELKEIDAALQG
                     VKRRGLDWVDVTREDFPLPAFAPGLAKIAQELETGRGMILLRGLPLAYSPDDLRTVYW
                     GIGAHLGTAVSQGGRGELLGIVEDEGKEVEVTRRRGSKTAGS"
     gene            complement(544..1296)
                     /locus_tag="AC158Q_03925"
     CDS             complement(544..1296)
                     /locus_tag="AC158Q_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3338949.1"
                     /translation="MSDGKVLIITGGSRGIGAATARLAAQRGYAVCVNYRANRAAADG
                     VVADIERAGGKAIAVAADVSSEPDVVRLFGTVDQSLGRVTALVNNAGTLEKQMRLEQM
                     DAARLTRAFAVNVIGSFLCAREAVRRMSTRHGGAGGAIVNVSSGAARLGSPNEYVDYA
                     ACKGAIDTMTIGLAHEVAAEGIRVNAVRPGYIYTEFHALGGEPGRVDRVKQHVPMKRG
                     GQPEEVANAIVWLLSDEASFSTGTFIDVTGGR"
     gene            complement(1296..2435)
                     /locus_tag="AC158Q_03930"
     CDS             complement(1296..2435)
                     /locus_tag="AC158Q_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR3338950.1"
                     /translation="MPKIQSVSVCVARVALDNATSFATRTVSARDYCLVKVRSTDGLE
                     GIGFCYVGSAGGNIARVAVEDLLAKKLIGQDSLRVEGLWQDMYSESLLQGRAGAVMRG
                     ISILDTALWDLNARAAKMPLHLFLGANVTDRVPAYASGGYYLAGKTPKMLAQEMASYV
                     KAGFKAVKMKVGRLSPKEEEVRIRAAREAVGPDIHLMLDANNAWSDVPTALTYMKRYE
                     PYDPYWIEEPFGPDEIDLHARLAELTPVVVATGEIAYGRWYHKELLDKRGAEIIQTDA
                     CVCGGITEWKRIAALAAGYGVTVCPHWFHDVHAPLVAATPNARFVEFFIDDQVLNFRR
                     LIDQQLAFRSGDLMLHQTPGLGFRFSERAVKKYALNRAKPWALIK"
     gene            complement(2692..3744)
                     /locus_tag="AC158Q_03935"
     CDS             complement(2692..3744)
                     /locus_tag="AC158Q_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HIR3338951.1"
                     /translation="MSAPRVNHERLLDFCAAVYCGAGMPEADARLVADTLVQADLWGH
                     QSHGVLRLGWYLDRVRNKVMNPVTKPEFVMDAGAIALIDGHDGVGHVLTVLATKEAVK
                     RARQHGIGAVGVRNSNHFGTCMYYTMMGARDGCVMFLTTNGGPAMAPWGGMKKIIGTN
                     PWSVAAPVGARAPFVVDMANTGVARGKIYLARNKRLPIPLGWAINAAGEPTTDPQEAI
                     DGIILPMAEHKGYAIAAMVDVLSGVLTGSGFLSSVHSPYKTAEKSNCGHLMIAMNIEA
                     FQPLAQFNASMEKFTAEIKAVPTAKGFDEVFYPGEIEANNDRRNRRNGIQFPDDTLAD
                     LKRIAGETGLMSKLPF"
     gene            complement(3754..>4387)
                     /locus_tag="AC158Q_03940"
     CDS             complement(3754..>4387)
                     /locus_tag="AC158Q_03940"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012357012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3338952.1"
                     /translation="SQVVVDTSTIDYSATLEMAAALEERGIGFIDAPVSGMEARAIEG
                     TLTAMCGGKAETLETVRPYLACVANSILHMGGAGSGQLAKLINQLLFDINCAALAEVL
                     PMAVKLGIDPEKIGKIVNSGTGKSYASEFFIPRILKNHFSDGYPLKHAYKDLVSGATL
                     GANMSVPMPVLAAATATYQMALLRGHGSLDKGAMIKVYEELLGVEFRSKQ"
BASE COUNT          677 a         1452 c         1451 g          807 t
ORIGIN      
        1 cgagccggcg gtcttggagc cgcggcggcg ggtgacctcg acctccttgc cctcgtcctc
       61 gacgatgccg agcagttctc cgcggccgcc ctgcgacacc gcggtgccga ggtgcgcgcc
      121 gatgccccag tagaccgtgc gcaagtcgtc gggcgagtag gccagcggga gccctcgcag
      181 caggatcatg ccgcgtccgg tctcgagttc ctgcgcgatc ttcgccagcc cgggcgcgaa
      241 tgccggcagc gggaagtcct cgcgcgtgac gtccacccag tcgagcccgc gccgcttcac
      301 gccctgcagc gccgcgtcga tttccttcag ctcggcgggc gcgaacggcc ggatccagtc
      361 cgtgcggtcc tggaggtccg cgcctttcca ggcgaagggg gcgacgatcg gtttatgttt
      421 cgctacctgc atggattgaa cggcgcgata agtgataggt gataggtgat aaggtttccc
      481 gtgcgctgcg cgggaaccgc atttataccc ctatcgcctg tcacttatca cctgtcacgt
      541 ctgtcagcgc ccgccggtca catcgatgaa ggtcccggtc gagaacgatg cctcgtccga
      601 gagcagccat actatcgcgt tcgccacttc ctccggctga ccgccgcgtt tcatcggcac
      661 gtgctgtttc acccggtcca cacggcccgg ctcgccgccc agcgcgtgga attcggtata
      721 gatgtagccc ggccgcaccg cgttcacgcg tataccctcg gcggcgacct cgtgggcgag
      781 cccgatggtc atcgtgtcga tcgcgccctt gcaggcggcg tagtccacgt actcgttcgg
      841 tgagcccagg cgcgcggcgc ccgaggacac gttgacgatg gcgccgccag ctccgccgtg
      901 acgggtggac atgcgccgca ccgcctcgcg cgcgcacagg aagctgccga tgacgttcac
      961 cgcgaaggcg cgggtcaggc gcgcggcgtc catctgctcg agccgcatct gcttctccag
     1021 ggtgccggca ttgttgacca gcgcggtgac gcgcccgagc gactgatcca ccgtcccgaa
     1081 cagccgcacg acgtcgggtt cggaggagac gtccgccgcg acggcgatcg ccttgccgcc
     1141 cgctcgctcg atgtcggcga cgacgccgtc agcggccgcc cggttggcgc ggtagttgac
     1201 gcagacggcg taaccgcgct gcgctgcgag ccgcgcagtc gccgccccga tgccgcggct
     1261 gccgccggtg atgatcagga ccttgccgtc actcatcact tgatgagggc ccagggcttc
     1321 gccctgttca acgcgtactt tttcaccgcg cgctcactga agcgaaaccc cagccccggc
     1381 gtctggtgca gcatcaggtc gccgctccgg aacgcgagct gctggtcgat caggcggcgg
     1441 aagttgagca cctggtcgtc gatgaagaac tcgacgaagc gcgcgttggg cgtcgcggcg
     1501 acgagagggg cgtgcacgtc gtggaaccag tgcgggcaga cggtgacgcc gtagcccgcc
     1561 gccagcgccg cgatgcgctt ccattcggtg atgccgccgc acacgcaggc gtcggtctgg
     1621 atgatctcgg cgccgcgctt gtcgagcagc tccttgtggt accagcgccc gtacgcaatc
     1681 tcgccggtcg cgaccaccac cggcgtcagc tcggcaagac gcgcatgcag gtcgatctcg
     1741 tcgggcccga acggctcctc gatccagtac gggtcgtagg gctcgtagcg cttcatgtag
     1801 gtgagcgcgg tgggcacgtc gctccaggcg ttgttggcgt cgagcatgag atggatgtcg
     1861 ggcccgacgg cctcgcgcgc ggcgcggatg cggacttcct cctccttcgg ggagaggcgc
     1921 ccgaccttca tcttgaccgc cttgaagccg gccttcacgt aggatgccat ttcctgagcg
     1981 agcatcttcg gcgtcttgcc ggcgaggtag taaccgccgc tcgcgtaggc cggcaccctg
     2041 tcggtcacgt tcgcgcccag gaagagatgg agcggcatct tcgcggcgcg cgcgttcaga
     2101 tcccacagcg cggtgtcgag gatggagatg ccgcgcatca cggccccggc gcgcccctgc
     2161 agcagcgatt ccgaatacat gtcctgccac agcccctcga cgcgcagaga gtcctggcca
     2221 atcagttttt tcgcgagcag atcctccacc gcaacccggg cgatgttgcc gcccgcgctg
     2281 ccgacgtagc agaagccgat gccttcgagc ccgtcggtcg agcgcacctt gaccaggcaa
     2341 tagtcgcgcg cgctgacggt gcgcgtggcg aaggaagtgg cgttgtcgag cgcgacccgc
     2401 gcgacgcaga cggagacgga ctggatcttc ggcatggtta tccatctttc aacaatcgaa
     2461 ccgccaagac cgccaagcac gccaaagcgg ggcggaacgg attaacttgt attgggggac
     2521 acttacctat tgaggaaaag tatataccgg tagcgaaaga tcactattag cagcgataga
     2581 tctccagtaa tagtgaacag gcctccttct ttgcgtcctc catattcgtg gctgaaggtt
     2641 tcttgatggg gttctcttgg cggccttggc gtcccggcgg ttcgatttga atcagaacgg
     2701 cagcttcgac atgaggccgg tctcgcccgc gatgcgcttc aggtcggcga gcgtgtcgtc
     2761 ggggaattga atgccgtttc tccggttgcg ccggtcgttg ttcgcctcga tctcgccggg
     2821 gtagaacacc tcgtcgaacc ccttggcggt tggcaccgcc ttgatctcgg ccgtgaactt
     2881 ttccatgctg gcgttgaatt gggcgagcgg ctgaaatgcc tcgatgttca tggcgatcat
     2941 gaggtggccg cagttgctct tctcggcggt cttgtacggg ctgtgcacgc tcgacaggaa
     3001 gccgctgccg gtcagcacgc cggagagcac gtccaccatc gccgcaatcg cgtagccctt
     3061 gtgctccgcc atcggcagga tgatgccgtc gatggcttcc tgcgggtcgg tggtcggttc
     3121 gcccgccgcg ttgatggccc agccgagcgg gatcggcagg cgcttgttgc gggcgagata
     3181 gattttcccg cgcgcgacgc cggtgttggc catgtccacc acgaacggcg cgcgggcacc
     3241 caccggcgcg gcgaccgacc agggattggt gccgatgatc ttcttcatgc cgccccaggg
     3301 cgccatcgcg gggccgccgt tggtggtgag gaacatgacg cagccgtcgc gggcgcccat
     3361 catcgtgtag tacatgcacg tgccaaaatg gttcgagttg cgcacgccga ccgccccgat
     3421 gccgtgctgc cgcgcgcgtt tcaccgcttc cttggtcgcg agcacggtca gcacgtggcc
     3481 gacgccgtcg tgaccgtcga tcagcgcgat cgcgcccgcg tccatgacga actcgggttt
     3541 cgtcacgggg ttcatgacct tgttgcgcac gcggtcgaga taccagccga ggcgcagcac
     3601 gccgtgcgac tggtgtcccc acaggtccgc ctgcaccagg gtgtcggcga cgaggcgcgc
     3661 gtcggcttcc ggcatgccgg cgccgcaata aacggcggcg cagaaatcga gcaggcgctc
     3721 gtggttgacg cggggagcgg acataggaat ctcctactgc ttcgagcgga actcgacgcc
     3781 cagcaattcc tcgtacacct tgatcatcgc gcctttgtcg aggctgccat gccccctcaa
     3841 cagcgccatc tggtaggtgg cggtcgccgc cgccagcacc ggcatgggca cgctcatgtt
     3901 cgcgccgagc gtggcgccgc tgacgaggtc cttgtaggcg tgcttgagcg ggtagccgtc
     3961 tgagaaatgg tttttcagga tgcgcgggat gaagaactcg gaggcgtagc tcttgcccgt
     4021 gccgctgttg acgatcttgc ctatcttctc cgggtcgatg ccgagcttca ccgccatcgg
     4081 cagcacttcg gcgagcgccg cgcagttgat gtcgaacagc agctggttga tcagcttggc
     4141 gagctggcca ctgccggcgc cgcccatgtg caggatgctg ttcgcgacgc aggcgaggta
     4201 cggccggacg gtctcgagcg tctcggcttt gccgccgcac atcgcggtga gcgtgccctc
     4261 gatggcgcgc gcttccatgc cggatacggg cgcatcgatg aagccgatgc cccgttcctc
     4321 cagcgcggcc gccatctcca gcgtcgccga gtagtcgatg gtgctggtgt ccaccacgac
     4381 ctgcgac
//
LOCUS       DBNGSG010000151         3314 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2767907, whole genome shotgun sequence.
ACCESSION   DBNGSG010000151 DBNGSG010000000
VERSION     DBNGSG010000151.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2767907"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="AC158Q_03945"
     CDS             complement(<1..343)
                     /locus_tag="AC158Q_03945"
                     /inference="COORDINATES: protein motif:HMM:NF019845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR3338953.1"
                     /translation="MRAMVLEQFGAPLKLKDVPVPKIGPNDALVRVRVCGAGLTVVIM
                     IASPGRIMSYPRIPGHEIAGEVVEVGPEVKTVKVGQRVTNHYYLTCGNCRYCRSGRET
                     LCENFRGNVGNG"
     gene            complement(560..1072)
                     /locus_tag="AC158Q_03950"
     CDS             complement(560..1072)
                     /locus_tag="AC158Q_03950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HIR3338954.1"
                     /translation="MYQLLKQIAVTGIKTEAAPQPDEALRVTADRLRADVLGYFAGAL
                     TIRHVDAGSCNGCELEINSLNNPYYNLEGLGIKFVASPRHADMLLVTGPVSRHMEVAL
                     KRTYDATPEPKLVVAIGDCGCTGGIFGESYASLGRVSNVIPVDVAVPGCPPTPLAIMQ
                     GILTAISVKK"
     gene            complement(1234..2793)
                     /locus_tag="AC158Q_03955"
     CDS             complement(1234..2793)
                     /locus_tag="AC158Q_03955"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase large subunit"
                     /protein_id="HIR3338955.1"
                     /translation="MKVEDFQIELKQLPGVMPVWHGRVDAQQWRSACEQVRNKGGRLV
                     ALWGADNSDRGQGRAVHAALTVQSGLLVITLPLRGGDCPSISDIFPAANRMQRAAFDL
                     LGISAAPSRDRRKWLRHAAWRADEFPLRKDFPVDSASANERHAAKETDAYPFVKVDGE
                     GVHEIPVGPVHAGTIEPGHFRFSIVGEGVLRLEERLGYKHKGIEKLFEQMDLRQGARL
                     AGRISGDSTAAYAWSYAMAVESACGMTPPPRALWLRALLLERERAGQHLWDLGFMGND
                     AGLAFGLAQFSRLREDMLRANAALFGHRYLMDAIVPGGVARDLDSDGMAAIRAEVDAL
                     GKTVALLKGIYDEHAGLQDRFIACGRVRPELAERLGLTGLAGRASGMAWDLRAQFPPV
                     PYDGFDVRMATHRNGDVAARATVRFEEIAESLRLIGLILAGLPAGDIAAPVPDAPDGA
                     FGIGWVEGWRGEVLIALETGADNRIRRLHPHDPSWQNWPLLERAVLGNIVPDFPLINK
                     SFNLSYSGQDL"
     gene            complement(2866..>3314)
                     /locus_tag="AC158Q_03960"
     CDS             complement(2866..>3314)
                     /locus_tag="AC158Q_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010810543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter transmembrane
                     domain-containing protein"
                     /protein_id="HIR3338956.1"
                     /translation="MTVHSLTKSAIFFAVGHAAQKSGTQVMDNIRGLIDSNPTIGWGL
                     MLGSLAILGMPPFGVFASEFMILTVTMHEQPWATPFLLVALGVAFAAIFGKVQPMVFG
                     AATTERLPHPPALIPVFVHLAIVLLLGLYIPPYLADWYRQAAALIG"
BASE COUNT          639 a         1081 c         1016 g          578 t
ORIGIN      
        1 agccattgcc gacgttgccc cggaagttct cgcacagggt ctcgcgcccg ctgcggcagt
       61 agcggcagtt gccgcaggtg aggtagtaat ggttggtcac gcgctggccg accttgacgg
      121 ttttcacctc gggcccgacc tccaccactt cgccggcaat ctcatggccg ggtatgcgtg
      181 ggtacgacat gatcctcccc ggcgaggcga tcatgatcac gaccgtcagc ccggcgccgc
      241 agacgcgcac cctgacgagc gcgtcgttcg ggccgatctt cggcaccggc acgtccttca
      301 gcttcagcgg cgcgccgaac tgctccaata ccatcgcccg catcgcccct cccgaggaaa
      361 cgaatggaca ggatcaactt gaatcgtcga acacccgcca aaacaaaaaa tagacaggat
      421 taacaggatt tacaagatta gatcaaatac aaaataagac aacgatcgga tgtttattct
      481 tgcattttta atcctgttaa tcttgttaat cctgtccatt aaagggtttg acgggttctt
      541 gatcccctgt tgatcctgtc tatttcttca cgctgatcgc agtcaggatc ccctgcatga
      601 tggcgagcgg cgtcggcggg catcccggca ccgcgacatc caccgggatc acgttcgaca
      661 cccgcccgag gctcgcgtag ctctcgccga agatgccgcc ggtgcagccg cagtcgccga
      721 tcgccaccac cagcttgggt tcgggcgtcg cgtcgtaggt gcgcttcagc gccacctcca
      781 tgtgccgcga aaccggcccc gtcaccagca gcatgtcggc atggcgcggg ctggcgacga
      841 acttgatgcc gagaccttcc aggttgtagt acggattgtt gagcgagttg atctcgagct
      901 cgcaaccgtt gcacgaaccc gcgtccacgt ggcggatggt gagcgcgccc gcgaaatacc
      961 cgagcacgtc cgcgcgcagg cggtcggccg tgacgcgcag cgcctcgtcg ggctgcggcg
     1021 ccgcctcggt cttgatgcct gtaacagcga tctgctttaa gagttgatac ataaattaac
     1081 cgccaaggcc gccaagaaaa aaaataatca taaaaaatag gatcacgggg cctgcaagat
     1141 gaccggccat atcgaacaag gagcgaaaag cgtacagatt atcaagtttc atattaattc
     1201 tgattctctt ggcggccttg gcggttcatt tttctacagg tcctgtccgc tgtaggacag
     1261 gttgaacgac ttgttgatca gcggaaaatc gggaacgatg ttgccgagca ccgcgcgctc
     1321 gagcagcggc cagttctgcc acgatggatc gtgcggatgc aggcggcgga tgcgattgtc
     1381 agcgccggtt tccagcgcga tcaacacttc gcctcgccag ccctcgaccc agccgatgcc
     1441 gaacgcgccg tccggcgcat ccggaacggg cgctgcgatg tcgccggcag gcaagccggc
     1501 aaggatcagg ccgatcagtc gcaatgactc ggcgatttcc tcgaagcgca ccgtcgcgcg
     1561 cgccgccacg tcgccgttgc ggtgcgtcgc catgcgcaca tcgaacccgt cgtacggcac
     1621 cggcggaaac tgcgcgcgca ggtcccaggc catcccgctg gcgcgcccgg caagcccggt
     1681 gagccccagc cgttcggcga gttcgggcct gacgcgcccg caggcaatga aacggtcctg
     1741 caagcccgca tgctcgtcgt aaatgccctt cagcagcgcc acggtcttcc cgagcgcatc
     1801 aacctcggcg cggatcgcgg ccatgccatc agaatcgagg tcgcgcgcca cacccccggg
     1861 cacgatggca tccatcaggt agcggtgacc gaacagcgcg gcgttggcgc gcagcatgtc
     1921 ctcccgcagg cgggaaaact gcgccaggcc gaatgcgagt cccgcgtcgt tgcccatgaa
     1981 acccaggtcc cacagatgct ggccggcgcg ctcgcgctcg agcagtagcg cgcgcagcca
     2041 cagcgcgcgc ggcggcggcg tcatgccgca cgcgctctcg accgccatgg cataagacca
     2101 cgcataggcc gccgtggaat cgccggaaat gcgcccggca agccgcgcgc cctgcctgag
     2161 gtccatctgc tcgaaaagtt tctcgatccc tttgtgcttg tagccgagcc gctcttcgag
     2221 gcgcaggacg ccctcgccga cgatggagaa gcgaaaatgt cccggttcga tcgtgcccgc
     2281 gtgaaccggg cctacgggaa tctcgtgcac gccctccccg tcgaccttca cgaatggata
     2341 ggcatcggtt tcttttgccg cgtggcgttc attcgccgat gccgagtcga cggggaaatc
     2401 tttccgaagc gggaattcgt cggcgcgcca ggcggcatgg cggagccact tgcggcggtc
     2461 gcgggaaggc gccgccgaga tgcccagcag atcgaaggcg gcgcgctgca tccggttcgc
     2521 cgcgggaaaa atgtcggata tgctgggaca gtctccgccg cgcagcggca gcgtgatcac
     2581 cagcagcccc gattgcacgg tcagcgcagc gtgcacggcg cggccctgcc cccggtcgct
     2641 gttgtcggca ccccacaagg caaccagacg gccgcccttg ttgcgcacct gctcgcaggc
     2701 cgaacgccat tgttgcgcat cgacgcggcc gtgccagacc ggcatcacgc ccggcagttg
     2761 tttcagctca atctggaaat cttcgacttt catggcgcga agggcggtac atcttccctc
     2821 cctctcaggg ggagggagca gagggtgggg gtggggttca tacgctcacc cgataagcgc
     2881 cgccgcctgg cgataccagt cggcgagata cggcggaata tacaagccga gcagcaacac
     2941 gatcgcaagg tgcacgaaca cgggtatcaa cgccggcgga tgcggcaggc gttccgtggt
     3001 cgccgccccg aaaaccatcg gctggacctt gccgaagatc gcggcgaatg ccacgccgag
     3061 tgcgacgagc aggaacggcg tggcccacgg ctgctcgtgc atcgtcaccg tgaggatcat
     3121 gaattcgctc gcaaacacgc cgaacggcgg catgccgagt atcgccaggc tgccgagcat
     3181 cagcccccag ccgatcgtcg ggttgctgtc gatcagcccc ctgatgttat ccatgacttg
     3241 cgtgccggat ttctgcgcgg cgtggccgac ggcaaaaaag atcgccgact tggtgagcga
     3301 atgcaccgtc atgt
//
LOCUS       DBNGSG010000152         4312 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3447133, whole genome shotgun sequence.
ACCESSION   DBNGSG010000152 DBNGSG010000000
VERSION     DBNGSG010000152.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4312)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3447133"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..975)
                     /locus_tag="AC158Q_03965"
     CDS             complement(<1..975)
                     /locus_tag="AC158Q_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HIR3338957.1"
                     /translation="MKRMLFNATQVEELRVAIVDGQKLIDLDIEIPGKEQRKSNIYKG
                     VITRIEPSLEAAFVDYGAERHGFLPFKEVARAYFKPGVDVGRATIKEALKEGQELIVQ
                     VDKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRVEGEDRNELRDVIDALPVP
                     QGMSVIGRTAAIGRAAEELNWDLNYLLQLWRAVESAAHQQTGAFLIYQESSLVIRAIR
                     DYYHQDIGEVLIDTEPVFEQAQQFMSHVMPQNVNRIKLYRDDVPLFSRFQIEHQIESA
                     YSRGVNLPSGGAVVFDHTEALVSIDVNSARSTRGSDIEETAFRTNLEAA"
     gene            1408..2349
                     /locus_tag="AC158Q_03970"
     CDS             1408..2349
                     /locus_tag="AC158Q_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR3338958.1"
                     /translation="MKHLSKDSATWHEVEPEAAGQRVDNYLTKWLKGVPKSHIYRILR
                     SGEVRINSRRIGPEYRIRPGDRLRLPPVRTAKPAATPKHPPAFRLKADVLYEDEALLV
                     IDKPAGTAVHGGSGISRGVIEQLRMERPEARFLELVHRLDRDTSGVLMLAKRRPALTD
                     LHRQLREGRVLKFYLTLVKGIWRDEKRSVKLALQKYVLASGERRVSVGRDGLASHTVF
                     LLKQARKDYSLLEAELKTGRTHQIRVHLAHIGYPIAGDDKYGDFALNKQLARKGLRRM
                     FLHASKTVITHPQSGAVLAFEARLPSELQVFLDALDT"
     gene            2333..3016
                     /locus_tag="AC158Q_03975"
     CDS             2333..3016
                     /locus_tag="AC158Q_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017424692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HIR3338959.1"
                     /translation="MRSTPDRRATARQFELLVFDWDGTLMDSAAAIAASLQAACCDLD
                     LPIPSEEQARYVIGLGFHDALSHLLPGLDPALYPPVLERYRDHFLKHDANTRLFPGAA
                     EALTALHDAGFLLAVATGKSRRGLERSLASTGLKGYFHATRCADEGHAKPHPGMLLEL
                     MEELGVASDRTLMIGDTTHDMAMARAAGVACLGVAYGAHSKSDLLGYAPLACVDDFDG
                     LQYWLARNT"
     gene            3107..4066
                     /locus_tag="AC158Q_03980"
     CDS             3107..4066
                     /locus_tag="AC158Q_03980"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HIR3338960.1"
                     /translation="MAELDTGGPGPNWERQTIEKVALAAVNEQRLARRWGIFFKLLAF
                     IYLFALLFIGLGWVGKKDTSPGRHTALVEINGVISSNSPASADSINAGLQEAFKDKRT
                     QGVILRINSPGGSPVQAGYINDEIRRLRAKHPNVPIYAVIEDICASGGYYIAVAADRI
                     YVDKASIVGSIGVLMDGFGFTGTMEKLGVERRLFAAGDNKGFLDPFAPGVESQKEHAQ
                     EMLGQIHQQFIGVVRQGRGKRLKETPDMFSGLLWVGQTSIELGLADALGSIEYVAREV
                     IKAEDIVDFTLRENVAERLAKKFGAGMAEGLAHFGAFQGMTLR"
BASE COUNT          806 a         1282 c         1395 g          829 t
ORIGIN      
        1 cgccgcctcg agattggtgc gaaatgccgt ttcctcgatg tcggacccgc gcgtggagcg
       61 ggcggaattg acgtcgatcg agaccagcgc ctcggtgtgg tcgaacacga ccgcgccgcc
      121 cgaaggcagg ttaacgccgc gcgagtatgc cgactcgatt tggtgttcga tctgaaagcg
      181 cgagaacagc ggaacgtcgt cgcgatagag cttgatgcgg ttgacgttct gcggcatgac
      241 gtggctcatg aactgctgcg cctgctcgaa gaccggttcg gtgtcgatca gtacctcgcc
      301 gatgtcctgg tggtagtagt cccggattgc ccggatcacc aggctcgatt cctggtagat
      361 caggaaagcg ccggtttgct gatgggcggc gctttcgacc gcacgccaca actggagcaa
      421 atagttgagg tcccagttga gctcttccgc cgcgcggccg atcgccgccg tgcggccgat
      481 tacgctcatg ccctgaggca caggcaacgc gtcgatgaca tcacgcaact cgtttcggtc
      541 ttcgccttcg acgcggcgcg acacgccgcc tccgcgcgga ttgttcggca tcagcaccag
      601 gtagcggccg gcgaggctga taaacgtggt gagcgccgcg cccttgttgc cgcgctcgtc
      661 cttgtccacc tggacgatca gctcctggcc ttctttcagc gcctccttga tcgtggcacg
      721 cccgacgtcg acgccgggct tgaagtaggc ccgcgcgact tccttgaagg ggaggaatcc
      781 gtggcgctcg gcgccatagt ccacgaaggc ggcctcgaga ctgggctcga tgcgggtgat
      841 aacacccttg tagatgttgc tcttgcgttg ttccttgccg gggatttcga tgtcgaggtc
      901 gatgagtttc tggccgtcaa cgatggcgac gcgcagctct tcgacctgcg tcgcgttaaa
      961 caacatgcgt ttcattgtta tcttctcccg cgctctaaca cgggatgggc aaaccacggc
     1021 gcctgcttta gggcaggcgg cgccgactga tcagtcgcgt ggcatgagca agtgggacat
     1081 ggagttcaga tgtctcgtgc ggtttgcctg agggatccgc tgcctggggc gcgggtcccg
     1141 gaattccgtt cggtgttgtg agcgcgtttt cgtctagtat cgctacttct gcatggtgag
     1201 cgacattaac cctgccttgc ggcgcattcg cggtcggcgc ggctttcctt tatgccgagc
     1261 gtccggagcc ggcgaatcgc ttcttgcacg cgtgtccatt tcttgcccgc ttgcaccgtg
     1321 gtgattggcg cctggcaagc cggccagcgg agcagttcgc ttcacgccgg caggcaacac
     1381 ttgaagtgca aagaatagta tatagaaatg aaacacttaa gtaaagattc ggcgacctgg
     1441 catgaagtgg agccggaagc ggccggccag cgagtggaca actacctgac caaatggctg
     1501 aaaggggtcc cgaaaagtca catctaccgc atcctgcgca gcggtgaagt gcgtatcaac
     1561 agccgccgga tcggccccga ataccggatc cggcccggcg accgcctgcg cctgccgccg
     1621 gtgcggaccg cgaagcctgc ggcgaccccg aagcaccctc cggccttccg gctgaaggct
     1681 gatgtcctct acgaggacga ggcgctgctg gtgatcgata agccggcggg caccgcggtg
     1741 cacggcggca gcggcatcag ccgcggggtc atcgagcagc tgcgcatgga gcggcccgaa
     1801 gcgcgcttcc tggaactcgt ccaccggctc gaccgcgaca cttcgggcgt gctgatgctc
     1861 gccaaacggc gcccggcgct gactgatctg caccggcagt tgcgggaagg gcgggtgctg
     1921 aagttctatc tcacgctggt caaaggcatc tggcgggacg aaaagcgcag cgtgaagctg
     1981 gcgctgcaaa aatacgtgct cgcttccggt gagcgccgcg tgagcgtcgg ccgcgacggg
     2041 ctcgcctcgc ataccgtgtt cctgctgaaa caggccagga aggactacag cttgctcgag
     2101 gcggaactca agactggccg cacacatcaa atccgggtgc atctcgccca tatcggctat
     2161 ccgatagcag gggacgacaa gtacggcgac tttgcgctca acaaacaact ggccaggaag
     2221 ggcctgaggc ggatgtttct gcatgcgtca aagacggtga tcacgcatcc ccaaagcggc
     2281 gctgtgctgg cgttcgaggc tcgattgcca tccgaactgc aggtgtttct agatgcgctc
     2341 gacacctgat cggcgcgcca cggccaggca attcgagttg ctggtgttcg actgggatgg
     2401 cacgctgatg gattcggcgg ccgcaatcgc cgcgtcattg caggcggcat gctgcgacct
     2461 cgatctcccg atacccagcg aggagcaagc gcgctacgtc atcgggctcg gatttcacga
     2521 cgcactgtcc cacctcctgc ccggactgga tccggcgctc tacccgccgg tgctggagcg
     2581 ctaccgcgac catttcctga agcacgatgc gaatacccgg ttatttcccg gggcggcgga
     2641 ggccctaacg gcactgcacg atgccgggtt tctgctggcg gttgcgaccg gcaagagccg
     2701 ccgtggcctc gagcggtcgc tcgccagcac aggcctcaaa ggctactttc acgcaacgcg
     2761 ctgtgcggac gaaggccatg ccaagcccca tcccgggatg ctgctagagc tcatggagga
     2821 actgggtgtc gccagcgaca gaacgctcat gattggggac acgacccatg acatggcgat
     2881 ggcccgcgcc gcaggcgtag cctgtctcgg cgtggcctac ggcgcgcact cgaaaagcga
     2941 cctcctgggg tatgcgccgc tcgcctgcgt cgatgatttc gatggcctgc agtactggtt
     3001 ggcgcgaaat acataaactg ccgcgaagcg ggggcaggat aacgctaaaa tagcgttgtc
     3061 gctgagggct ccacgccctg ctgcgatcct gactgaggac cgttatatgg ccgagctgga
     3121 tacaggtgga ccgggaccga actgggagcg gcagacgatc gaaaaggtcg cgctggctgc
     3181 agtcaacgag cagcgcctcg cgcggcgctg gggcatcttc ttcaagttgc tcgcgttcat
     3241 ctatttgttc gcactgctgt tcattggact gggctgggtc ggcaagaagg atacgagccc
     3301 cggaaggcat accgccttgg tggagatcaa cggggtcatc tcgtccaaca gtcctgccag
     3361 cgcggatagc atcaatgccg ggttgcagga agcgttcaag gataagcgta cccagggggt
     3421 gattctgcgc atcaacagtc ccggcggcag cccggtccag gccggttaca tcaatgatga
     3481 aatccggcgc ttgcgggcca agcatcccaa tgtcccgatt tatgcggtga tcgaggatat
     3541 ctgcgcctcc ggcggctatt acatcgcggt ggccgctgat cgcatctacg tggacaaggc
     3601 aagcatcgtc ggctcgatcg gcgtgctgat ggacggcttc ggattcaccg gcaccatgga
     3661 aaagctgggt gtcgagcgcc ggttgtttgc tgcgggggac aacaaaggct ttctcgaccc
     3721 gttcgctccc ggggtcgaat cgcaaaagga gcatgcccag gaaatgctgg gccaaatcca
     3781 ccagcagttc atcggcgtgg tccggcaggg acgcggcaag cgtctcaagg aaacgccgga
     3841 catgttctcc gggttgctgt gggtcggcca gacgagcatc gagcttggcc tggccgatgc
     3901 gcttggcagc atcgaatacg tcgcgcgcga agttatcaag gccgaggata tcgtcgattt
     3961 cacgctgcgg gagaacgttg cggagcgcct ggcgaaaaag ttcggcgccg gaatggccga
     4021 aggcctggcg cattttggcg cttttcaagg catgacgctg cgctgaggcc ggggaagaaa
     4081 gggagggtga tgcctccttt tgatccctca caaacgttca agaattcagt ctggcgagtt
     4141 cacagcgata attcagttct gtcctcggga tagcaggaac acgacgagcc gccggtcaag
     4201 atccggcggc ttttttttcc actcgccgat ggaacgcgtc gccacggatt cggcctgcag
     4261 cgtgagatcg gccgcaatgc agagcagggt atcctggcgg cagtcttcca gc
//
LOCUS       DBNGSG010000153         3860 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1409123, whole genome shotgun sequence.
ACCESSION   DBNGSG010000153 DBNGSG010000000
VERSION     DBNGSG010000153.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3860)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1409123"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1631)
                     /locus_tag="AC158Q_03985"
     CDS             complement(<1..1631)
                     /locus_tag="AC158Q_03985"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004065 - arylsulfatase activity [Evidence
                     IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HIR3338961.1"
                     /translation="MSMARAIGRYLTAAAVLAAALLAGCGPEPGDATGLSAADLAGSR
                     PNIILIMADDMGFSDLGSYGASVINTPNIDGLAGDGLRFTQFYNAARCVPTRASLLTG
                     LYPHQAGIGHMVGRSGNGLYDGEFSRNAITLAEGLGSAGYDTYMSGKWHLTPWLPGSG
                     AMAADGPAGRGFDRSYGTIMSVRSYFNPPSLMEDGRELPATEGDYHYTDAISDKAVEY
                     IEQQDPDKPYFLYVAYAAAHFPLQAREEDIARYRGRFLAGWDALREQRYQRLVELDLI
                     DPAWPLPDADPRQLRWDEVDPAYLAWFDERMAVYAAMIEQMDRGVGRIIDAVEARGEL
                     ENTIIIFLSDNGACAEEIELGQALGGGVARQTRSGEPVRLGNDPSIFPGPEDTYASVG
                     LEWAAYSNTPFRSFKSFVHEGGIATPLIVSWPGQLESRIVREPGHIIDIMPTLLELAG
                     ASYPAEFNGYQIQPPEGRSLLPLFAGESRPDAVYVWEHEGNRAIRDGDYKLVSSFGNA
                     WELYDLRVDRLEANDLSGEMPDRLAAMIERYEAEA"
     gene            complement(1628..2740)
                     /locus_tag="AC158Q_03990"
     CDS             complement(1628..2740)
                     /locus_tag="AC158Q_03990"
                     /inference="COORDINATES: protein motif:HMM:NF024981.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HIR3338962.1"
                     /translation="MASLHRTALYGSLSLLVVSVVLFAVDMPLAAGPSLTAAFALAAF
                     GVRGAEKLRGISFSLLIFAAVTLAMSYPAPFFSWGEFRLSTLIEPLLQIIMFGMGTAI
                     SAKDFVGVVRNPRAVGVGLACQYTIMPFVGAGLALTFDLEPEIAAGIVLIGAAPSGLA
                     SNVMAYIAKANLALSVTLTTLATLLAPLLTPFYMQWLAGQLVPIDALDMMWDITRITI
                     LPIVAGLVFNHFLHGRTAWLDRLMPQLSMGGIVLILMIMTAGGRDNLLEIGMLLIMIS
                     LLHNISGYVLGYWTCRLLRFDERTCRTISFEVGLQNAGLATGLATAMGKAATLGLAPT
                     VFGSLMNITGATLATYWRDQPLRDEPAARDGKAEAA"
     gene            complement(2802..>3860)
                     /locus_tag="AC158Q_03995"
     CDS             complement(2802..>3860)
                     /locus_tag="AC158Q_03995"
                     /inference="COORDINATES: protein motif:HMM:NF024771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR3338963.1"
                     /translation="MVDTDIGITGIGEGGTPDSLAPTAARLIGKNPFEITRLWQDMYR
                     SYHYPPGKERLHAMGALDLALWDLKGKALDAPLYELLGGKVRDYLETYTTGFRGTGTT
                     LQEHARQCIEAGYRFYRFDAASVQGTNIYDARERVVRVAEDCAAAREGVGPYGNFMVD
                     FHQRFSFSDALRCCKMIEDTYPYFVEDPVETNSFQQDIPRLRQMTSVPLGAGEEWGYR
                     YDLIELVENHDLDFIRISLPNVGGITELVRVMAICESHDVGIVPHFTGPIATAAQAHA
                     LATFPFSVVFEYNYQTRQIPYLQQNWITFNEGKVYTNDRPGLGVEVDTDSLRLVATYT
                     EANNRPMYYRPDGSPITW"
BASE COUNT          718 a         1224 c         1257 g          661 t
ORIGIN      
        1 tcggcctccg cctcgtagcg ctcaatcatt gcggctagcc tgtccggcat ttcgcctgac
       61 aggtcgttcg cttcgagacg atcgacgcgc aggtcgtaca gctcccaggc gttgccgaaa
      121 ctgctgacga gcttgtaatc gccgtcccgg atcgccctgt tgccttcgtg ttcccagacg
      181 tatacggcgt ccggtcgcga ttcgccggca aacagcggca aaagactgcg accctcgggc
      241 ggctggatct gatagccgtt gaactccgcg ggatagctgg ccccggccag ctcgagcaaa
      301 gtcggcatga tgtcgatgat atgacccggc tcgcgcacga tgcgcgattc gagctgaccg
      361 ggccaggaca cgatcagcgg cgtcgcgatg cccccttcat gtacgaagct cttgaaggag
      421 cgaaacggcg tattcgagta ggcggcccat tcgagtccga ccgaagcata ggtgtcctcc
      481 ggccccggaa agatcgacgg gtcattgccc aatcggaccg gctcgccgga ccgtgtctgc
      541 cgggcgacgc cgccgccgag cgcctgtccc agttcgatct cttcggcgca ggcgccgttg
      601 tccgaaagga aaatgatgat cgtgttttcg agctcaccgc gggcttcgac cgcatcgatg
      661 atccggccga cgccgcgatc catctgctcg atcatcgccg cgtagacggc catccgctcg
      721 tcgaaccagg ccaggtacgc cggatcgact tcgtcccagc ggagttgccg cggatccgcg
      781 tccggcagcg gccacgcggg atcgatgaga tcgagctcga ccagccgctg ataacgctgt
      841 tctcgcagtg catcccatcc cgccaggaaa cggccgcgat agcgggcgat gtcttcctcg
      901 cgcgcctgca gcggaaagtg cgcggccgcg taggcgacat agagaaagta cggcttgtcc
      961 ggatcctgct gctcgatgta ctcgacggcc ttgtcggaaa tcgcatcggt gtagtgatag
     1021 tcgccttccg tcgccggcag ctcgcgcccg tcctccatca gcgacggcgg gttgaagtaa
     1081 ctccggacgg acatgatcgt gccgtaggag cgatcgaaac cgcgcccggc cggaccgtct
     1141 gcggccatcg cgccggaccc cggaagccag ggcgtcaggt gccatttgcc ggacatgtag
     1201 gtgtcgtagc cggcgctgcc gaggccttcg gccagggtga tggcattacg cgagaactca
     1261 ccgtcataca ggccgttgcc ggatcgtccg accatgtggc cgattcccgc ctggtgcgga
     1321 tacagtccgg tcaggagact cgcacgggtc ggcacgcagc gtgcagcgtt gtagaactgc
     1381 gtgaagcgca ggccatcgcc cgccagacca tcgatattcg gtgtattgat caccgacgca
     1441 ccgtagctgc cgagatcgga gaaacccatg tcgtcagcca tgatcaggat gatgttgggc
     1501 cggctgccgg caagatccgc ggcggacagg ccggtcgcat cgcccggttc cggcccgcag
     1561 cccgccagca gcgctgcggc gaggacagcg gcggcggtga ggtaccggcc gatggcgcgc
     1621 gccatgctca tgcggcctcc gccttgccgt cccgtgccgc gggctcgtca cgcagcggct
     1681 ggtcgcgcca gtaggtggcc agcgtggcgc cggtgatgtt catcagcgag ccgaataccg
     1741 tcggcgcgag gccgagcgtt gccgccttgc ccatggccgt ggcgagtccg gtcgcaagcc
     1801 cggcgttctg caggccaacc tcgaacgaga tcgtgcgaca ggttcgttcg tcaaaacgca
     1861 ggagccgaca ggtccagtag ccgagcacgt agcccgatat attgtgcagc aggctgatca
     1921 tgatcagcag catcccgatc tcgagcaggt tgtcgcggcc accggccgtc atgatcatca
     1981 ggatcaagac aatgccgccc atcgagagtt gcggcatgag ccggtcgagc caggcggtcc
     2041 ggccatgcag gaagtggttg aacacgaggc cggcaacgat cggcaggatc gtgatccggg
     2101 tgatgtccca catcatgtcc aacgcatcga tcgggaccag ctggccggct agccattgca
     2161 tgtagaacgg cgtgagcagc ggcgcaagca gggtcgcaag ggtcgtcagg gttaccgaca
     2221 gcgcgagatt cgccttggcg atataggcca tcacgttcga ggcgagcccg ctcggtgcgg
     2281 cgccgatgag cacgatgccc gcggcgatct cgggttccag gtcgaaggtc agcgccagac
     2341 cggcaccgac gaacggcatg atcgtgtact ggcaggcgag gccgacaccg acggcgcgcg
     2401 gattcctgac cacgccgacg aaatccttcg cgctgatcgc cgtgcccatg ccgaacatga
     2461 tgatctgcag cagcggctcg atcagggtgc tgagccggaa ttctccccag ctgaagaaag
     2521 gcgccggata gctcatcgcc agcgtgacgg cggcgaagat cagcaggctg aaggagatgc
     2581 cccggagctt ttccgcgccg cggacaccga atgccgccag ggcgaaggcg gcggtcaatg
     2641 acgggcccgc agcgagcggc atgtcgacag caaacagtac gaccgacacg accaggagcg
     2701 agagcgagcc gtacaaggcg gtcctgtgca acgaagccaa gtctgtagcg atcgtcaatg
     2761 taaacaggat gccatcacga ggctgtcgcg acgtcgcgag attaccaggt gatcggcgaa
     2821 ccgtccggcc ggtagtacat cgggcgattg ttcgcctcgg tgtatgtagc taccaggcgc
     2881 agcgaatcgg tatcaacctc gacgccgaga cccggacggt cgttcgtgta gacctttcct
     2941 tcattgaagg tgatccagtt ctgctggaga tacggaatct gccgcgtctg gtagttgtac
     3001 tcgaacacga cgctgaaggg gaaggtcgca agcgcgtgcg cctgtgccgc cgtcgcgatc
     3061 ggacccgtga agtgcggcac gatgccgacg tcgtgcgact cgcatatcgc catcacgcgg
     3121 acgagctccg tgatgccacc cacgttcggg agcgagatcc ggatgaagtc aaggtcatgg
     3181 ttctcgacga gctcgatgag gtcgtagcgg tagccccact cttcgccagc cccgagcggc
     3241 acgctcgtca tctggcgcag tctcgggatg tcctgctgga acgagttcgt ctccacggga
     3301 tcttcgacga aatacggata cgtgtcctcg atcatcttgc agcagcgcag cgcatcggag
     3361 aaggaaaagc gctggtggaa gtcgaccatg aagttgccgt agggaccaac cccctcgcgg
     3421 gccgcggcgc aatcctcggc cacccgcacg acccgctcgc gagcgtcgta aatgttggtt
     3481 ccctgaacgc tcgctgcgtc gaagcgatag aagcggtacc ccgcctcgat acactgccgg
     3541 gcgtgctcct gcagggtggt gcccgtcccc cggaaacccg tcgtgtaggt ctcgaggtag
     3601 tcccggactt tgccgcccag gagctcgtac agaggcgcat ctagcgcctt gcctttgaga
     3661 tcccagagcg cgagatccag cgcgcccatc gcatgcagac gctccttgcc gggcgggtaa
     3721 tggtaggagc gatacatgtc ctgccacagc cgcgtgatct cgaacgggtt cttgccgatc
     3781 agccgcgcgg cggtgggtgc gagcgagtcg ggcgtgccac cctcgccgat acccgtgatg
     3841 ccgatgtccg tatcgaccat
//
LOCUS       DBNGSG010000154         5631 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3108497, whole genome shotgun sequence.
ACCESSION   DBNGSG010000154 DBNGSG010000000
VERSION     DBNGSG010000154.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5631)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3108497"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1046)
                     /locus_tag="AC158Q_04000"
     CDS             complement(<1..1046)
                     /locus_tag="AC158Q_04000"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HIR3338964.1"
                     /translation="MNFKQALNIDDLRIIARQRLPRIAYEFLARGTEDEVTLRANRSA
                     FERIRFVPRTMVDVSKRSQQKVLWGKTFNSPFGIAPTGASGLYCFDADVALGQAAEAA
                     GVPYVLSTASFVPMERVAREAGGTKWFQLYMSKDLELAERLVTRARDAGFEALVMTTD
                     IPVAGNREYNQRNHFEVPFRLNLANMADGLLHPRWLAGVFLRTLLATGVPRFQNLDVN
                     TGGRITSPTLTEFRTRRTELNWTDFAWLRKLWPGKLLVKGIMTVEDAQLAAQHGADGI
                     FVSNHGGRQLDGAPSPIEVLPPIARAVGERMAIMLDGGIRRGTDVVKALALGADMVFV
                     GRATLFGATAGGRAG"
     gene            1241..1726
                     /locus_tag="AC158Q_04005"
     CDS             1241..1726
                     /locus_tag="AC158Q_04005"
                     /inference="COORDINATES: protein motif:HMM:NF024106.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative molybdenum carrier protein"
                     /protein_id="HIR3338965.1"
                     /translation="MTLRKIVSGGQTGVDRGALDAALGAGFPCGGWVPAGRIAEDGPI
                     AARYPVSELPGSGYEERTIRNVQDSDGTAILYFGALEGGTRLTMDYCVGHDKPCELLN
                     AERLSPGEAAREIAAFIERNGITVLNVAGPRASKWPGAHAYAKRTIETLLKSSAATEK
                     G"
     gene            1786..2901
                     /locus_tag="AC158Q_04010"
     CDS             1786..2901
                     /locus_tag="AC158Q_04010"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3338966.1"
                     /translation="MYLILLLCISVHACYIGSKVVVTLLAVNLGASPATIGVLAACYG
                     VAPLLLGVHSGRLSDSAGTRPPMIAGAVMTAAAMLCGYFFQTIASLMVVAVLMGMGFV
                     YYNVAVQNYTGTHGDSAHRARNFSWLSMSYSVSAFIGPVFAGYAIDYSGHGAAFLGFA
                     LFSAVPLVTLALNRAFGAFPGRPANASQQSTYDLLRTPPIRRVVIMSGLMVSVWELYI
                     FYMPLHGHSIGLSASTIGIVLGIYAVAALTVRFLLPIILRYTTTLRLVSVAMIGAAVV
                     FVALPWIRQVALLMAASFTIGLLLGICQPVMMMLAFERSPAGRTGEVTGLRLISNNVA
                     RIVVPLIGGALGAAFGAAPVFWMNAVSLAAISWLARR"
     gene            complement(3066..3689)
                     /locus_tag="AC158Q_04015"
     CDS             complement(3066..3689)
                     /locus_tag="AC158Q_04015"
                     /inference="COORDINATES: protein motif:HMM:NF024809.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HIR3338967.1"
                     /translation="MKLYITPGSPYARMARIVVLEKGLESRVEIIAAQTRLADSPYYR
                     INPSGRVPYLVRDDGVGLEESSVICAWLDHLDAKPAFDLPAGDPAWEARRLEALARSM
                     LDGLAVWSREILRPQVEHSPAVVQHEAHRSRRMADLWEAEISHPLMRGALNMTQITLA
                     CALGLDARIPDFQWRPNHPRLSAWYDAIAARPSFAATAPPGASASAR"
     gene            complement(3789..4199)
                     /locus_tag="AC158Q_04020"
     CDS             complement(3789..4199)
                     /locus_tag="AC158Q_04020"
                     /inference="COORDINATES: protein motif:HMM:NF015648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HIR3338968.1"
                     /translation="MSYIEQSLSAGERVEGLFKLHWFAWVPMVMWIILGIPTLGLTWI
                     IALYEFLRLRFLEQGVTNKRVILKRGIISRKTEEMKLTSIETVEIDQGIIGRLFGFGT
                     VKITGRGISDFVFKGINDPMSVKRQIESVSNPAG"
     gene            complement(4533..5432)
                     /locus_tag="AC158Q_04025"
     CDS             complement(4533..5432)
                     /locus_tag="AC158Q_04025"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR3338969.1"
                     /translation="MTEQKTSMAVLFADVSDSTRLYEALGDTVAFGNVREVIALLKGI
                     AETCEGRVVKTIGDGLMCAFPSADCAARAAADMQKQIASRPPMNSGRQLTIRVGFHFG
                     PVIRDGEDVFGDSVNGAARMAGLALSGQALTDASTVAALSAQLREATRQINALPVKGK
                     AEAVEVHELLWQTSSDRTVIPGRAAGEPASVPRSGPCITLTHQGRETPYTDTIYFGRE
                     AAGNHVVVADPMASRRHAKIELRAGRFVLLDQSSNGTYVVLGNNAELQLKREEMILYS
                     SGKFAFGHSTTSPGAEVIEFRCS"
BASE COUNT          959 a         1841 c         1751 g         1080 t
ORIGIN      
        1 cccgccctgc cgcccgcggt cgcgccgaag agcgtggcgc gcccgacgaa caccatgtcc
       61 gcgccgagcg cgagcgcctt gacgacgtcc gtgccgcgcc ggatgccgcc gtccagcatg
      121 atggccatgc gctcgccgac tgcccgtgcg atcggcggca gcacctcgat cggcgagggc
      181 gcgccgtcga gctggcgccc gccgtggttg gagacgaaga tgccgtcggc gccgtgctgc
      241 gccgcgagct gcgcgtcctc aaccgtcatg atgcccttta ccagcagctt ccccggccac
      301 agcttgcgca gccaggcgaa gtctgtccag ttcagctcgg ttcgccgcgt gcggaattcc
      361 gtcagcgtcg gggacgtgat gcgcccgccg gtgttgacgt ccaggttctg gaagcgcggc
      421 acccccgtgg caagcagcgt gcgcaggaac acgccggcga gccagcgcgg gtggagcagc
      481 ccatcggcca tgttggcgag attcaaccgg aacggcacct cgaagtggtt cctctggttg
      541 tactcgcggt tgccggccac cgggatgtcg gtggtcatca ccagggcttc gaagccggca
      601 tcccgcgcgc gggtgaccag ccgttccgcc agctccaggt ccttggacat gtagagctgg
      661 aaccacttcg ttcccccggc ctcccgggcg acgcgctcca tcggcacgaa cgaggcggtg
      721 gacagcacgt agggcacgcc ggcggcctcc gccgcctgcc cgagcgcgac gtcggcgtcg
      781 aaacagtaga gccccgatgc gccggtcggc gcgatgccga acggagaatt gaatgtcttg
      841 ccccaaagca ccttctgctg ggagcgcttc gacacgtcca ccatcgtgcg cggaacgaag
      901 cggatgcgtt cgaacgcgga ccggttggcg cgcagcgtca cctcgtcctc ggtgccgcgg
      961 gcgagaaact cgtacgcaat gcgcggcagc cgctgccggg cgatgatgcg gagatcgtcg
     1021 atgttgaggg cctgcttgaa gttcacgggc tggactttac cgcccgccgc gcataggcga
     1081 ggcaggtcta aagtaaactg gcgctattgt aattcacgcg gcgggctttc gttactcgtt
     1141 actcgttctt cgtttctcgt tgctcgtcca ctcgtaattg ccccggctgc aaggctttcg
     1201 cgacacgaga aacgagtaac gaacaacgag tcactttttc atgactttga ggaaaatcgt
     1261 ctccggcgga cagaccggcg ttgaccgcgg cgcgctcgac gccgcgctcg gcgcgggttt
     1321 tccctgcggc ggctgggtgc ctgcaggacg aatcgcggag gacggcccga tcgccgcgcg
     1381 ctacccggtg tccgaactgc ccggcagcgg ctacgaggag cgcacgatca ggaacgtgca
     1441 ggacagcgac ggcaccgcca tcctctactt cggcgcgctg gaaggcggca cgcgtctcac
     1501 catggattac tgcgtcgggc acgacaagcc gtgcgagctg ctcaacgccg agcgcctgag
     1561 tcccggggag gccgcccggg aaatcgcggc attcatcgag agaaacggca tcacggtgct
     1621 gaacgtcgcc ggcccgcgcg cgagcaagtg gcccggcgcc cacgcctacg ccaaacggac
     1681 catcgagacc ttgttgaagt cgagcgcggc aaccgaaaag gggtgagcag ccggatgtct
     1741 ggtctcactc atcacttatc acttatcact tatcacttat cactgatgta cctgatcctc
     1801 ctcctctgca tttcggtgca cgcctgctac atcggcagca aggtggtggt gacgctgctc
     1861 gccgtgaacc tcggcgcgag cccggccacg atcggcgtgc tcgccgcgtg ttacggcgtg
     1921 gcgccgctgc tgctgggcgt gcactcgggg cgcctgtccg acagcgccgg cacgcgcccg
     1981 ccgatgatcg cgggcgcggt gatgaccgcg gcggccatgc tctgcggcta ttttttccag
     2041 accatcgcaa gcctgatggt ggtcgcggtg ctgatgggca tgggcttcgt ctattacaac
     2101 gtcgccgtac agaactacac cggcacccat ggcgactccg cgcaccgcgc gcgtaatttc
     2161 agctggctgt ccatgtccta ctcggtgtcg gcgtttatcg gcccggtgtt cgcgggctac
     2221 gccatcgatt acagcggcca cggagccgcg tttctcggct tcgccctgtt ttccgcggtg
     2281 ccgctcgtca ccctggcact caaccgggcc ttcggggcct ttcccggcag gcccgcgaac
     2341 gcgtcacagc aaagcactta cgacctgctg cgcaccccgc ccatacggcg ggtcgtcatc
     2401 atgagcgggc tcatggtctc ggtgtgggaa ctctatatct tctatatgcc cctgcacggt
     2461 cactcgatcg gtctgtccgc ttcgacgatc ggcatcgttc tggggatcta tgccgtcgcc
     2521 gcgctgaccg tgcgcttcct gctgccgatc atcctgcgct acacgaccac gctgcgcctg
     2581 gtatcggtcg cgatgatcgg cgcggcggtg gtattcgtgg cgctgccgtg gatccgccag
     2641 gtcgcgctgc tgatggccgc ctcgttcacg atcgggctgc tgctgggcat ctgccagccg
     2701 gtgatgatga tgctcgcctt cgagcgctcg cccgcggggc gcaccggcga agtgacgggg
     2761 ctgcgcctga tctccaacaa cgtcgcccgc atcgtcgtgc ccctgatcgg cggcgcgctg
     2821 ggcgcggcgt tcggggcggc gccggtgttc tggatgaacg cggtgagcct ggcggcgatc
     2881 agctggctgg cgcggcgtta ggtattaatt aaccgccaag gcctgtcctg agcaagtcga
     2941 agggacgcca aggccgtcaa gaaatacaaa ataaatcaga ttgacttaat gaaacgccga
     3001 gagaaacaag aataagaatt ggcggccggt gaaggccgat caatacaacc tgagggccga
     3061 aattatcagc gcgcgctggc tgacgcgccg ggcggcgcgg tggctgcgaa tgatgggcgc
     3121 gcggcgatgg cgtcatacca ggcagacagc ctggggtggt tcgggcgcca ctgaaaatca
     3181 ggaatccggg cgtcgagccc gagggcgcag gcgagcgtga tctgcgtcat gttgagcgct
     3241 ccgcgcatca gcggatgact gatctccgcc tcccatagat cagccattcg tcggctgcgg
     3301 tgcgcttcgt gctggacgac cgcgggcgaa tgctcgactt gcggcctcag gatctcgcgg
     3361 ctccagaccg cgagcccgtc cagcatgctc cgcgccaggg cttcgaggcg ccgcgcttcc
     3421 cacgccgggt caccggccgg caaatcgaat gccggtttgg cgtcgaggtg atcgagccag
     3481 gcgcagatga cggaggattc ttccaacccg actccgtcgt cacggacgag atagggaacc
     3541 cggcccgatg gattgatgcg gtagtacggg ctgtccgcaa gacgcgtctg tgccgcaatg
     3601 atctcgaccc ggctctccag ccccttctcg agaacgacga tccgcgccat gcgcgcgtag
     3661 ggagaccccg gggtaatgta gagcttcatt tatttcgatg agcgacagga acccaattct
     3721 tgagaagtcc ttcttcgaac gccatgatac gttcttgcgc ataaaaggtt cctggaacct
     3781 tttatgcgtc agcctgccgg gttgctcacg ctttcgatct gtcgcttcac ggacatggga
     3841 tcgtttatgc ctttgaatac aaaatcgctg attccgcggc cggtgatctt caccgtccca
     3901 aacccgaaca ggcgaccgat tatgccctgg tcaatctcta cggtctcgat ggacgtaagc
     3961 ttcatctctt ccgtcttgcg actaatgatg cctctcttga gaatcactcg tttgttcgtg
     4021 acgccctgct ccaggaacct cagcctcaag aattcgtaca gggcgataat ccaggtcagt
     4081 ccgagcgtgg ggatcccaag gatgatccac atcaccatgg ggacccatgc gaaccaatgg
     4141 agcttgaaaa gcccttcgac cctctcacca gcggacaacg actgttcgat ataggacacg
     4201 ggttctccca tgcggtggat ggtttcctga gtgttaacgt gacatggaaa ccctgaaagg
     4261 tggatgacat tctgcctgct ttgaaggccg gaaagtaatt tgtttcttga atcgagatgg
     4321 tacacctgaa caggttcacc gtatctgcct gcctgcatac gctttgaacg gcgagcttgc
     4381 cgcctgttcg aacgcccgcg gcctccgaat cgaagaaggc aagtatccag ccacaacgtg
     4441 tcggccgcct tgcgcgattc tgcggccgca ttctcatgaa tggtgccctg ctccgcgctc
     4501 cggcgcgcca aaccggcacg aagtgcccgg atctaactgc agcggaattc gatgacttcc
     4561 gcccccgggc tggtggtgct gtggccgaag gcgaattttc cactcgagta gaggatcatc
     4621 tcctcgcgct tcaactgcag ctccgcgttg ttgccgagca cgacgtaggt gccgttgctg
     4681 ctctggtcca gcaacacgaa cctgccggcc cgcagctcga tcttggcgtg gcgccgcgaa
     4741 gccatgggat ccgccaccac cacgtggttt ccggcggcct cccgcccgaa gtagatggtg
     4801 tcggtgtacg gagtttccct cccctgatgc gtcagcgtaa tgcaggggcc gctgcggggc
     4861 acggaagcgg gctcccccgc ggcacggccc gggatgaccg tgcggtcgct gctcgtctgc
     4921 cacagcagct cgtggacctc gaccgcctcc gcctttccct tcaccggcag ggcgttgatc
     4981 tgtcgcgtgg cctcgcgcaa ctgggcggac agcgccgcca ccgtggaagc atccgtgagc
     5041 gcctgcccgg agagcgcgag tcccgccatg cgcgccgcgc cgttcacgct gtcaccgaac
     5101 acgtcctccc cgtcccggat taccggcccg aaatgaaagc ccacgcggat cgtcaattgc
     5161 ctgccgctat tcatcggcgg gcgcgaggcg atctgtttct gcatgtcagc ggcggccctg
     5221 gccgcgcagt cggccgacgg aaacgcgcac atcaggccgt cgccgatggt cttcaccacc
     5281 cttccttcgc acgtctcggc gatgcccttc agcagcgcga tcacttcgcg cacattgccg
     5341 aaggccaccg tgtctccgag cgcttcgtag agacgcgtgc tgtcgctcac gtcggcgaac
     5401 agcacggcca tgctggtttt ctgctccgtc aagacttccc tccgcggccg gggaaagtct
     5461 agcaggttca ttgatttttc cggaactccc gcgccgtcag cgtgtcgcct gaaaaggagc
     5521 cggaccggac cgtccggtgt tttcaactca tcactccgga gccatcagaa tcccatgaaa
     5581 cgcgccaccg ctttcttttt tcctttggcc ttagcaactt gttccgtttt c
//
LOCUS       DBNGSG010000155         5952 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1071418, whole genome shotgun sequence.
ACCESSION   DBNGSG010000155 DBNGSG010000000
VERSION     DBNGSG010000155.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5952)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5952)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5952
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1071418"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..177
                     /locus_tag="AC158Q_04030"
     CDS             <1..177
                     /locus_tag="AC158Q_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HIR3338970.1"
                     /translation="CAEMCSTKALLGGDGDVLADIFRTRVTTRGKGSEVWGWGTAYGK
                     PDKPQAAPPAGAKS"
     gene            174..410
                     /locus_tag="AC158Q_04035"
     CDS             174..410
                     /locus_tag="AC158Q_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338971.1"
                     /translation="MKRMLAVAVAAGLTVIASGCGEGTQVTVYKQGQYQGKPDKRPWE
                     NDLFKGDKLAWEQAIKARNERQDETSRGTPTAKN"
     gene            499..1497
                     /locus_tag="AC158Q_04040"
     CDS             499..1497
                     /locus_tag="AC158Q_04040"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HIR3338972.1"
                     /translation="MQPAWAAATGGAQEQAERQQSQPLNNAPVWRDVRGGDNAYQTTQ
                     VRGIETNVLVQTQGEIWRQIRNGPITVFGGYLLILAALAILAFYLWKGPIKVHGQLTG
                     RKIRRFSAWDRTMHWTVAISWLILAISGLVLLFGKYVLLPVFGYTLFSWLAILSKNLH
                     NFVGPVFLIAAALMFFTYLGRNIPAAHDITWLLKGGGMLSGKHVPSGFFNAGEKIVFW
                     IGLFLFTIIVGASGLVLLFPNLDQGRVIMQYANIVHAVTAVVYIAMMLGHMYLGSVGL
                     EGAYENMRHDGLVDETWAKEHHEYWYNEVMKGQPGTGAAGTVSAARAGAMKEGWKL"
     gene            1494..1742
                     /locus_tag="AC158Q_04045"
     CDS             1494..1742
                     /locus_tag="AC158Q_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338973.1"
                     /translation="MKAPKFWMVIPALMIGGAVGLASAKLPQAPMDDAAKAKAAAAKA
                     KAAEGAKKARAAEEKAMDRAVERYKKEKGMTTASAKKK"
     gene            complement(1801..3327)
                     /locus_tag="AC158Q_04050"
     CDS             complement(1801..3327)
                     /locus_tag="AC158Q_04050"
                     /inference="COORDINATES: protein motif:HMM:NF013132.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR3338974.1"
                     /translation="MSAATVRRIGLFLGPLLGLFCYYLLPPHYSTGPGEWVEFTQAGR
                     STLALMVWMATWWLTEAVDIEVTALLPIVTFPLFGVAPLSKVLPPYAADVIFLFMGGF
                     IIGLAIERWGLDRRIAFFTLRLVGARPGAIIGGFMAVTAFLSMWVSNTACAAMMVPIA
                     MSVIDLVLRSRTGVGLKDGGIPRDRVPERNFATGLLLCIAYSASIGGIATIIGSPPNG
                     LAVRYIQQTFQKEVSFFDWLMIGGPFTLIFLPIAWLLMTRFLFRSDIGEIAGGRQHFD
                     AEYRKLGALTRGEKVVLAVFSVTAFLWIFSPLLKGVVIADVKPLAALSDAVIAMLAAM
                     ALFLIPVDPAKGERAMDWDTAVKLPWGVLMLFGGGLTLATAIEANGVSEFIGNSSRGL
                     SGLHPLVLLLAITAMTVFLSELTSNTAQVATMVPVLAAMAPVLGMNPYVLILACTLGA
                     SSAYMMPVGTPPNAIVFGTGLIRLPQMMKAGFWLNLAGILVITALAWLFITPLLGNVY
                     "
     gene            complement(3362..4813)
                     /locus_tag="AC158Q_04055"
     CDS             complement(3362..4813)
                     /locus_tag="AC158Q_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HIR3338975.1"
                     /translation="MKISWKAIAPIAVAILIALIPAPAGLPQHAWYFFAIFVGVIVGL
                     MFEPLPGGAIGLIGVTVVAVLSEWVFFSPEQLAKPGFNSARASLSWALSGFSNTTVWL
                     IFGAFMFALGYEKTGLGRRIALLLVKAMGRKTLTLGYAVTIADLLLAPFTPSNTARSG
                     GTIYPVIRNLPPLYDSKPNDPSARRMGSYLMWVALAATCVTSSMFLTALAPNLLAIEL
                     VRKTAQVEFTWMQWFTAFAPVGILLLVLVPLLTYWLYPPEVKEGAEVPAWAAKELEKM
                     GPISQREMTLGVLVLIALVLWIFGGKIMEATTAAITVISLMLLLKVVTWDDITANKAA
                     WNTLAWFATLVALAAGLTSVGFVKWFAETVAGQLSGVSPTVAIAALLLINFFGHYLFA
                     SVTAHVTAMIPVLLAVASTIPGVNMHTLALGLCLQLGIMGVITPYATGPSPVYYGCGY
                     LPSADYWRLGAIFGVIFFLAFLVIGVPWMLFTR"
     gene            complement(4845..5201)
                     /gene="frdD"
                     /locus_tag="AC158Q_04060"
     CDS             complement(4845..5201)
                     /gene="frdD"
                     /locus_tag="AC158Q_04060"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HIR3338976.1"
                     /translation="MKRSHEPIFWSLFGAGGVLSALIAPILIVITGIITPIGLWMPQQ
                     ALAYSRVLEFAQHWLGKALLLAVISLFLFHAAHRIYHGLHDVGVHGGTGAMVAAYGGA
                     LVGTLVSAYLLLSIGF"
     gene            complement(5203..5595)
                     /locus_tag="AC158Q_04065"
     CDS             complement(5203..5595)
                     /locus_tag="AC158Q_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit C"
                     /protein_id="HIR3338977.1"
                     /translation="MAKVKTFVRPMEGWWRKNPYFVRYMIRESSSVFLAIYAVILLVG
                     LLRLTQGEAVYEAWRAALTSPVSILFHWLALLTVGYHAYTWWKVAPKTAPDIRVAGRA
                     IPECVITGGGWLATIGASILVYIIVRWV"
     gene            complement(5595..>5952)
                     /locus_tag="AC158Q_04070"
     CDS             complement(5595..>5952)
                     /locus_tag="AC158Q_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3338978.1"
                     /translation="THLQTPAQFQKYYQYSQCINCLLCYAACPQYGLKPEFTGPAALA
                     LLHRYNADSRDRGWEERAELLNAEEGVWGCTLVGYCSEVCPKHVDPAHAINQNKVNST
                     MDYFGVRKLLMPKGGD"
BASE COUNT         1216 a         1815 c         1984 g          937 t
ORIGIN      
        1 tgcgccgaga tgtgctcgac caaggccctg ctgggtggag acggcgacgt gctcgccgac
       61 atcttccgca cgcgtgtgac cacgcgcggc aaaggtagcg aggtatgggg ctggggcact
      121 gcctacggca agcccgacaa gccgcaagcc gcgccgccgg ctggagccaa gtcatgaagc
      181 ggatgctcgc tgtcgcggta gccgcgggat tgaccgtgat cgcgtcgggg tgcggtgagg
      241 gcacgcaggt gacggtgtac aagcaggggc agtaccaggg gaagccggat aaacgaccct
      301 gggagaacga cctcttcaag ggcgataaat tggcgtggga acaggcgatc aaggcgcgta
      361 acgagcgcca ggatgaaact tcgcgcggca cccccacggc gaagaactga atcaaaccca
      421 aaggaggatc gcatggtcag cattcgcgac atgcgtggat taattagatt cgcttgcgca
      481 ttgctcgcac tgatttgcgt gcagcccgcc tgggccgctg caaccggagg cgcccaggag
      541 caggcggagc gccagcagtc gcaaccgctc aataacgcac cggtatggcg cgacgtgcgg
      601 ggtggcgaca atgcttacca gaccacccag gtgcggggca tcgagaccaa cgtgctggtg
      661 cagacccagg gcgagatctg gcgccagatc cgcaacgggc cgatcacggt tttcggcgga
      721 tacctgctga tcctcgccgc actggcgatt cttgccttct acctgtggaa ggggccgatc
      781 aaggtccacg gccagctgac cggcaggaaa atccggcgct tctcggcgtg ggaccggacg
      841 atgcactgga ccgtggccat cagctggctg atcctcgcga tttccggcct ggtgctgctg
      901 ttcgggaagt acgtcttgtt gccggtgttc gggtacacgt tgttttcgtg gctggccatc
      961 ctgtcgaaga acctgcacaa cttcgtgggc ccggtgttcc tgatcgccgc cgccctcatg
     1021 ttcttcacct acctcggccg caatatcccg gcggcgcacg acatcacctg gctgctcaag
     1081 ggcggaggga tgctctcggg caagcacgtg ccgtccggct ttttcaacgc gggcgagaag
     1141 atcgttttct ggatcggatt gttcctgttc acgatcattg tcggcgcgtc gggcctggtg
     1201 ctcctgttcc ccaacctgga tcagggtcgc gtgatcatgc agtacgccaa catcgtccac
     1261 gcggtgacgg ccgtggtcta catcgcgatg atgttgggcc acatgtacct gggctcggtc
     1321 ggtctcgaag gcgcctacga gaatatgcgt catgacgggc tggtggacga gacctgggcg
     1381 aaggaacacc acgagtactg gtacaacgag gtcatgaagg gccagcccgg tacgggcgcg
     1441 gcgggcaccg tatccgccgc ccgcgcgggc gccatgaagg aggggtggaa gctatgaagg
     1501 caccgaaatt ctggatggtc ataccagcgc tgatgattgg cggcgcggtg gggctggcat
     1561 cggccaagct gccgcaagcg ccgatggacg atgccgccaa ggccaaggct gccgcggcca
     1621 aagccaaggc agccgaaggc gccaagaaag cgcgggcggc cgaggaaaaa gcgatggacc
     1681 gggcggtcga gcgctacaag aaagaaaagg gcatgacgac cgcgtcggct aaaaagaaat
     1741 agaacattcc cgccaaacaa aaagcccggg gatcccgggc ttttttcttg atcctgcccg
     1801 ctagtaaacg ttgccgagca gcggcgtaat gaagagccat gccaacgccg tgatgaccag
     1861 gatgccggcg aggttaagcc aaaagcccgc cttcatcatc tggggcagcc gtatcaggcc
     1921 cgttccgaac acgattgcat tgggtggcgt tcccacgggc atcatgtagg cgctgctggc
     1981 gcccagcgtg caggccagta tcaggacata gggattcatg ccgagcaccg gcgccatggc
     2041 cgcaagcacc gggaccatgg tggcgacctg ggcggtattg gaggtcaact ccgacaggaa
     2101 taccgtcatg gcggtgatgg cgagcaacag cacgagggga tgcagcccgg agaggccgcg
     2161 gctcgagttg ccgatgaact ccgatacgcc gttcgcctcg atggcggttg cgagcgtgag
     2221 gccaccgcca aaaagcatca gcacgcccca gggcagcttg accgccgtgt cccagtccat
     2281 tgcgcgctcg cccttggcgg ggtccaccgg aatcaggaac aacgccatcg ccgcgagcat
     2341 ggcgatcacc gcgtccgaga gcgcggcaag cggctttacg tccgcgatca cgacgccctt
     2401 gagcaggggg ctgaatatcc agagaaaggc ggtgacgctg aataccgcca gcacgacttt
     2461 ttcaccgcgg gtgagggcgc ccagcttgcg gtactccgcg tcgaaatgct gccgcccgcc
     2521 cgcgatctcg ccgatgtccg agcgaaaaag aaagcgtgtc atcagcagcc aggcaatcgg
     2581 aaggaagatg agggtgaacg gcccgcctat catcagccag tcgaaaaagc tcacttcctt
     2641 ctggaaagtc tgctggatgt agcgcaccgc gaggccgttg ggcggggagc cgatgatggt
     2701 ggctattccc ccaatcgatg ccgagtaggc aatgcacagc agcagcccgg tggcgaaatt
     2761 gcgctccggg acgcggtccc gcggaatccc cccgtccttc aggccaacgc cggtgcggct
     2821 gcgcaatacg agatcgatga cgctcatcgc gattggtacc atcatggcgg cgcacgcggt
     2881 attcgagacc cacatgctca agaaggccgt gacggccatg aagccgccga tgatggcgcc
     2941 cgggcgcgcg ccgaccagtc gcagcgtgaa gaaagcgatg cgccggtcga ggccccagcg
     3001 ctcgatggca agcccgatga tgaacccgcc catgaacagg aagatcacgt ccgcggcata
     3061 gggcggaagg accttgctca ggggagcgac gccgaacaac gggaaggtga cgatcggcag
     3121 cagggcggtg acttcgatgt cgaccgcctc cgtgagccac caggtggcca tccagaccat
     3181 cagggccaat gtggagcgcc cggcctgggt gaactccacc cactcgcccg ggccggtcga
     3241 gtagtggggc ggcaataggt aatagcagaa cagccccagc agcggcccta ggaacaggcc
     3301 gatgcgcctt acggtcgcgg cggacacgga aaggccaggg gctgtatgcg ccaggctccg
     3361 ctcaccgcgt gaacagcatc cagggcacgc cgatcaccag gaatgcgagg aagaagatca
     3421 cgccgaatat cgcgcccagc cgccagtagt ccgccgacgg cagatagccg cagccgtagt
     3481 acaccgggct tgggcccgtt gcgtacgggg tgatgacgcc catgatgccg agctgcaggc
     3541 acagccccag tgcaagcgtg tgcatgttca ccccgggaat ggttgacgcc accgcgagca
     3601 ggaccgggat catggcggtg acgtgcgcgg tgacgctcgc gaacaggtag tgcccgaaga
     3661 aattgatcag cagcagtgcg gcgatcgcca ccgtcggcga gacgccgctc agctgcccgg
     3721 cgacggtttc ggcaaaccat ttcacgaatc cgacgctggt caggccggcg gcaagggcaa
     3781 ccagcgtcgc gaaccacgcc agcgtgttcc acgccgcctt gttagccgtg atgtcgtccc
     3841 aggtgacgac cttgaggagc aacatgagcg agatgacggt aatggcggcg gtggtagctt
     3901 ccatgatctt cccgccgaag atccacagca ccagcgcgat caacaccagg acgccgagcg
     3961 tcatctcgcg ctggctgatg gggcccatct tttccagttc ttttgccgcc caggcgggca
     4021 cttcggcccc ctccttgacc tcgggcggat agagccagta ggtgagcaac ggcaccagca
     4081 cgagcagcag gatgccgacc ggagcgaaag ccgtgaacca ctgcatccac gtgaactcga
     4141 cctgcgcggt tttcctcacg agttcgatgg cgagcaggtt gggcgcgagc gcggtgagga
     4201 acatcgagct cgtcacgcaa gtcgcggcga gcgccaccca catgagatac gagcccatgc
     4261 gccgcgccga gggatcgttc ggtttcgaat cgtagagggg cggcaggttg cggatcaccg
     4321 gatagatcgt gccgccgctg cgggccgtgt tggacggggt gaaaggagcg agcagcaggt
     4381 cggcgatcgt caccgcgtag cccagcgtga gtgtcttgcg gcccatggcc ttcaccagca
     4441 gcaacgcgat gcgccggccc aggccggtct tctcgtagcc cagcgcgaac atgaacgcgc
     4501 cgaagatcag ccagaccgtc gtgttggaga agcccgacaa cgcccagctc agcgaggccc
     4561 gggccgagtt gaagccgggc ttggcgagtt gctcggggct gaagaagacc cattcggaca
     4621 gcacggctac caccgtgacg ccgatgaggc cgatcgcgcc ccccggcagc ggctcgaaca
     4681 tcagaccgac gatgacgccg acgaagatcg cgaagaaata ccacgcgtgc tggggcaatc
     4741 cggcgggcgc ggggatgagc gcgatcagga tggcgacggc gatgggagcg atggctttcc
     4801 aagagatttt catgttgctc tcctttgagt ccgtcgaacc ttctctaaaa ccctattgaa
     4861 agcaataagt aggcagacac gagcgtgccg accagtgcgc cgccgtaagc agcgaccatg
     4921 gccccggtac cgccgtgcac gccgacgtcg tgcaggccgt ggtagatgcg gtgcgcggcg
     4981 tgaaagagga aaagcgagat caccgcgagc agcagcgcct tgccgagcca gtgctgcgcg
     5041 aactcgagca cccgcgaata ggcgagggcc tgctgcggca tccacagccc gatgggcgtg
     5101 atgatgcccg tgatgacgat gaggataggg gcgatgagcg cggaaagcac gccgccggcg
     5161 ccgaacaggg accagaaaat cggttcgtgg gatcgtttca tgtcacaccc accgcacgat
     5221 gatgtagacc aggatggatg cgccgatcgt cgccagccag ccgccgccgg tgataacgca
     5281 ctccggtatg gcgcggcccg cgacccggat atccggcgcg gtcttgggcg ccactttcca
     5341 ccaggtatac gcgtggtaac cgacggtcag cagcgcgagc cagtgaaaga ggatcgagac
     5401 gggactcgtc agcgccgcgc gccacgcctc gtagaccgcc tcgccctggg tgagccggag
     5461 cagccccacc agcaggatga cggcatagat cgcgaggaat acgctgctgg actccctgat
     5521 catgtagcgc acgaagtaag ggttcttgcg ccaccaccct tccatcgggc gaacgaaagt
     5581 cttgaccttg gccatcagtc gcctcccttc ggcatcagca gcttgcgcac accaaaatag
     5641 tccatggtgc tgttgacctt gttctggttg atggcgtgcg ccgggtccac gtgcttcggg
     5701 cagacctcgg agcagtagcc gaccagggtg cagccccaaa ccccttcctc ggcgttgagg
     5761 agctcggcgc gctcttccca gccgcggtcg cgcgagtcag cgttatatcg gtggaggaga
     5821 gcgagcgccg ccgggcccgt gaattcaggc ttgaggccgt actgcggaca ggccgcgtag
     5881 cacagcaggc aattgatgca ctgtgagtac tggtagtact tctggaactg cgccggtgtc
     5941 tgcagatgcg tg
//
LOCUS       DBNGSG010000156         1851 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_731693, whole genome shotgun sequence.
ACCESSION   DBNGSG010000156 DBNGSG010000000
VERSION     DBNGSG010000156.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_731693"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="AC158Q_04075"
     CDS             complement(<1..215)
                     /locus_tag="AC158Q_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338979.1"
                     /translation="MKSSAERAIGTLPRPVLALLVVAFCLQVAWQALQPAPSARAEAL
                     GDPPTASALRAAALGEPIALAQFLTLYL"
     gene            complement(421..1326)
                     /locus_tag="AC158Q_04080"
     CDS             complement(421..1326)
                     /locus_tag="AC158Q_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3338980.1"
                     /translation="MAVLIAFSLRGLADGVFRYTVCGYSSDFVTTHDSRITIHAFHNC
                     GMDTRITTIARYTLLEALRTRLPALVLAALLALLAASFFIESISITEGARFQAGFYAA
                     GMRLACVFIMAITVLVSVTRELNDKGLDVALALDLPRSHYILGKLAGFLAIAVLIAAT
                     ASLPLAWLAAPQAAMQWMISLWLELAIVVALALFCILTFSQLTPAASFVLAFYLLARS
                     LTAIRLMGAHPLAGADTLSHQVIQFLVEALALIVPAFDAWTRTAWLVNEPAAWSALLL
                     LAGQSAIYVALLAAAAMFDFYRKNF"
     gene            1313..>1851
                     /locus_tag="AC158Q_04085"
     CDS             1313..>1851
                     /locus_tag="AC158Q_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338981.1"
                     /translation="MSTAMGASTRMIVAYPQWWLGLMLLALHASLAWGIADLWSRAFL
                     LAHFGLFLLWQPVWRGEKNIESRHAFLVVIVGFLLAAWNNWWLMAVWLAVLFGLIGGS
                     VPGITERRQRLASMLAALYLLSMLLIWIVPHLFPGQVIEDAMELLVKYGLPVLPVAIL
                     LVRVESGRVASPVAVDLFY"
BASE COUNT          368 a          543 c          619 g          321 t
ORIGIN      
        1 agatacagcg tcagaaactg ggcgagcgcg atgggctctc ccagcgcggc cgcgcgcagg
       61 gcggacgccg tgggcggatc gccgagcgcc tccgcccgcg cgctcggcgc gggctgcagc
      121 gcctgccacg cgacctgcaa acagaacgca acgaccagca gcgccaacac tggccgcggc
      181 aaggttccga tcgcgcgttc cgcgctgctt ttcattcgcg cttcgtcagg aagagacagg
      241 attcaaagtg aaatatcaaa acccgtcaac gccgtaaatg gacaggattc acaagattaa
      301 caagaataga actcacaaaa gcgagaagag gttttgattg aatcctgtaa atcttgttaa
      361 tcctgtctat taatgggttt gacgggtcta cggttttcga tcaggaaatc atgtctattt
      421 ctagaagttt ttccgataaa agtcgaacat cgccgccgcg gcaagcagcg caacgtagat
      481 cgcgctttgc cccgccagca ggagaagggc ggaccaggcc gccggctcgt tgacgagcca
      541 cgcggtccgg gtccacgcat cgaatgcagg cacgatcagc gccagggctt cgaccaggaa
      601 ctggatcacc tggtgcgaga gggtgtccgc gccggccagg gggtgggcgc ccatcagccg
      661 tatcgccgtc agcgagcgcg caagcaggta aaaggccagc acgaaacttg cagcgggagt
      721 gagctggctg aaggtcagga tgcagaacag ggcaagcgcg acgacgatgg caagctcgag
      781 ccagagggaa atcatccact gcatcgccgc ctgcggggcg gcgagccagg cgagcggcag
      841 ggaggcggtc gcggcgatca aaaccgcgat cgccaggaat cccgccagct tgcccaggat
      901 gtaatgcgat cgcggcaggt cgagggcgag cgccacgtcg agccccttgt cgttcagttc
      961 gcgcgtcacg ctgaccagca cggtgatggc cataataaaa acgcaggcga gccgcatgcc
     1021 cgcggcatag aaccccgcct ggaagcgcgc gccttcggtg atcgagatgg attcgatgaa
     1081 aaaactcgcc gccagcagcg cgagcagggc ggccagcacc agcgccggca gacgggtgcg
     1141 cagcgcttcg agcaaggtgt agcgcgcgat ggtcgtgatt cgggtatcca tgccacaatt
     1201 atgaaaggcg tgaatcgtga ttcgtgagtc gtgagtcgtg acgaaatcgg atgaatagcc
     1261 gcagacagta tagcgaaaga ccccgtccgc gagaccgcgc agggagaacg cgatgagcac
     1321 ggccatgggc gcatccaccc ggatgatcgt ggcctatccc cagtggtggc tcgggctgat
     1381 gctgctcgcg ctgcacgctt cgctcgcctg ggggatagcc gatttgtggt cgcgcgcttt
     1441 cctgctcgcg catttcgggc tgtttctgct gtggcagccg gtgtggcgcg gcgagaagaa
     1501 catcgaatcg cgccacgcgt tcctggtggt catcgtcggg ttcctgctgg cggcgtggaa
     1561 caactggtgg ctgatggcgg tgtggctcgc cgtgctgttt ggcctgatcg gcggttccgt
     1621 ccccggcatc accgagcggc ggcagcggct ggcctcgatg ctggcagcgc tctatctcct
     1681 gtccatgctg ctgatctgga tcgtgccgca cctgttcccc ggccaggtca tcgaggacgc
     1741 gatggaactg ctggtgaagt acgggctgcc ggtactgccg gttgccatcc tgctggtccg
     1801 ggtcgagagc ggccgcgtgg cgtcaccggt cgcggtggac cttttctaca g
//
LOCUS       DBNGSG010000157         9443 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2260272, whole genome shotgun sequence.
ACCESSION   DBNGSG010000157 DBNGSG010000000
VERSION     DBNGSG010000157.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9443)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9443)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9443
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2260272"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1626)
                     /gene="yidC"
                     /locus_tag="AC158Q_04090"
     CDS             complement(<1..1626)
                     /gene="yidC"
                     /locus_tag="AC158Q_04090"
                     /inference="COORDINATES: protein motif:HMM:NF002352.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HIR3338982.1"
                     /translation="MPDSGVQCGGGRGNSQTSGRVLRPATRTGRRAFPVKKVMDSQRL
                     ILFFVFSFSVFLLLDAWQRDQQPAQPAVVQGDKAAKAPSPSAQVPVPGDKLKVPQATV
                     PEAARSVPAAGATIQVETDLLIARIGIEGGDLRHLQFKKHRDILDRNKDFVLFEISPE
                     RTYVAQSGLIGNGLPNHRTTYQATSTSHKLADGQDTVEVRLRAPPADGVQVDKVYRFR
                     RGSYLIDFSFEITNKGAAVVQPYAYFQIVRDGKPPVGDSAMLPTFTGMAVYTEKEKFQ
                     KLAFEDVAKNKAPYPKTSNDGWIAVLQHYFFSAWLPKDGTPREFYTRKLDENLYAAGV
                     IVPDGTLGAGASATLAVPLYAGPQEQENLAKIAPGLDLTIDYGWLTVIAVPLFWVLAR
                     LYDWTGNWGVAIILLTVIIKLLFYPLSAASYRSMAKMRVLAPKLQRLKEQYGDDRQRM
                     QQAMMELYKTEKINPLGGCMPILVQIPVFIALYWALLASVELRHAPFMLWIEDLAAPD
                     PWFILPILMAVTMWIQTKLNPEPPDPVQAKVMKI"
     gene            complement(1521..1907)
                     /gene="rnpA"
                     /locus_tag="AC158Q_04095"
     CDS             complement(1521..1907)
                     /gene="rnpA"
                     /locus_tag="AC158Q_04095"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HIR3338983.1"
                     /translation="MSAGAPATLPRSARLLRREQFTEALASGAAGRRRHFTLFVRPNG
                     LSLARMGIIASKRVAPRAVDRNRAKRLVREIFRRLRHRLEGVDVVVELRRCPTPGFNV
                     EAGAEIARLLEESCARPHGQGGGPSR"
     gene            complement(1904..2038)
                     /gene="rpmH"
                     /locus_tag="AC158Q_04100"
     CDS             complement(1904..2038)
                     /gene="rpmH"
                     /locus_tag="AC158Q_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HIR3338984.1"
                     /translation="MKRTYQPSKTRRKRTHGFLVRKRTRGGRAVLRARRAKGRARLSV
                     "
     gene            2231..3565
                     /gene="dnaA"
                     /locus_tag="AC158Q_04105"
     CDS             2231..3565
                     /gene="dnaA"
                     /locus_tag="AC158Q_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HIR3338985.1"
                     /translation="MNSFWRFCLARFEKELPPQQYQTWIKPLRFHADGDRLTLVAPNR
                     FVLQWIRDKFLPRIELLAREQLKRNIEVLLTLSEKEHAATPPALPPLQAPKDRGAKPS
                     SREVSRLNPAFAFDTFVTGKANELARAAAAQVAERPGAAYNPLFIYGGVGLGKTHLIQ
                     AIGNRLREQAPETKIRYIHAEQYVSDVVRAYQHKAFDDFKRYYHSLDLLLIDDIQFFS
                     GKSRTQEEFFYAFNALIEAHKQVVITCDTYPKEIAGIENRIISRFGWGLTVAVEPPEL
                     EMRVAILLKKAEVEGVRLDEAVAFFLAKHIQSNVRELEGGLKRVLAYARFSGRDLTAE
                     LCREALRDLLAIQHRQVSIENIQRTVADYYKIKVSEMYSKKRSRNVARPRQMAMALAK
                     ELTQLSLPDIGDAFGGRDHTTVLHACRKIALLKSTNSELTRDFNSLLNVLRS"
     gene            3765..4874
                     /gene="dnaN"
                     /locus_tag="AC158Q_04110"
     CDS             3765..4874
                     /gene="dnaN"
                     /locus_tag="AC158Q_04110"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HIR3338986.1"
                     /translation="MKLVQLERDALLRPLQAVTGIVEKRHTLPILSNVLIERKKNRLL
                     LVATDLEIQVATSTETGKEADGDTSLTVSARKLQDILRALPEATEATLDVQNNRLQVR
                     AGKSRFNLQTLPSSDFPAMADPGAAQAKVSMPQKALRELLLLVQFAMAQQDIRYYLNG
                     LLLVLDGGLVRVVATDGHRLSYAARPLGQQQEKREVILPRKAVLELGRLLGDGEEPVS
                     IEIFAGLVRFSFGNVVLTTKIIDGKFPDYTRVVPTNYQKRFSIKRQELLQSLQRAAIL
                     SNEKFRGVRWMVATNSLRISCTNNEQEEAQEELEITYGEEALDIGFNITYLLDVLNNV
                     HSDNVECSFGDANSSMLVTVPDIQEFRYVVMPMRI"
     gene            4885..7317
                     /gene="gyrB"
                     /locus_tag="AC158Q_04115"
     CDS             4885..7317
                     /gene="gyrB"
                     /locus_tag="AC158Q_04115"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HIR3338987.1"
                     /translation="MTKEAIERESLRSADDYDSSSIKVLKGLEAVRKRPGMYIGDTGD
                     GTGLHHMVFEVVDNAVDEALAGFCTEITVTIHTDNSISVVDNGRGIPTDLHPEDEAGR
                     SSAEVVMTELHAGGKFDNNSYKVAGGLHGVGVSVVNALSEWLRLSIRRDGQLHQMEFR
                     MGETVAPLKATGKSERRGTEVHFLPSKEVFGRIEFHYEILAKRLRELAFLNSGLDITL
                     VDQRSGKEEKFLFTGGVKGFVEYMNRSKNVLHPNVFYARAEKDGIVVEVAMQWNDSYQ
                     ESMQCFTNNIPQRDGGTHLTGLRAAMTRTLNQYIETSKLAEKAKVETAGDDMKEGLTA
                     VLSVKVPEPKFSSQTKDKLVSSEVRPVVDEIVAQKLHEFLLERPGDARIICSKIIDAA
                     RAREAARKARELTRRKGVLDSFGLSGKLADCQERDPAQCELYIVEGDSAGGSAKQGRD
                     RKFQAILPLKGKILNVERARLDKLLSSQEIITLISVLGTGIGKEDYAPEKLRYHRIII
                     MTDADVDGSHIRTLLLTFFYRQMPELIERGHVYIAQPPLYKAKHGKTERYLKDEHELK
                     QFLLKLALSEAELFTAVNGQTLSKDALEMVAKEYLLAEAVIERVSRLVDEDVLHALLR
                     QPVQLDLSDEKAARNSAQALAALLAPMGMMVEAKYDEKAERYALVVNRTQHGIVRHCY
                     IDADFVQGGDYDQIQRTAKVLEGLLAEGAYVKRGEQQQPVREFREAIEWLLGEVQRGA
                     SIQRYKGLGEMNPEQLWETTMNPKTRLLLKTQIEDAISAEEIFSTLMGDQVEPRRNFI
                     EANALGVRNLDI"
     gene            complement(7355..>9443)
                     /locus_tag="AC158Q_04120"
     CDS             complement(7355..>9443)
                     /locus_tag="AC158Q_04120"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HIR3338988.1"
                     /translation="AMTAFNSKEGGFYLTTVGRVQTPTLAILVEREERIRAFRSRDYW
                     EVHAKFGARAGEYPGRWFDPVFKKDDDAERRAERQWSAAEAEAITAFCRGKTGTVDEE
                     TKPATQISPLLFDLTSLQREANGRFGFSARNTLGLAQALYEKHKVLTYPRTDSRALPE
                     DYIGTVKKAMDMLGGQREYAPFAKSVLKNGWVRPNKRVFDNSKISDHFAIIPTLQAPR
                     HLSEPEHKLYDLVVRRFLAVFHPAAEYLQTTRITQVGEHHFKTEGRILQTPGWLAVYG
                     RSAQEESQTLPPITAGEKVEVLEINAIANQTRPPARYSEATLLSAMEGAGKLVEDDEL
                     RAAMEAKGLGTPATRAAVIEGLIREAYVHREGRELKPTWKAFRLLLALHHFGVNEITS
                     PELTGDWEHKLKQMEAGELKRDVFMDHIEQVTRDLVERVRNGDIPDEAYATVEAPCPK
                     CGGMVQENYRKFQCQKCDFSLWVVLSGREWAPEEIAELIQKRFIGPLNGFRSRMGKPF
                     SAGIRLSSDLKIEFDFGQVRLEEEAANPPDFSNQDSLGPCPKCGARVFEYGTVYVCEK
                     AVGAERACDFRSGKMILQQPIERDQMQKLLTTGRTDLFTRFISKKGRPFKAYLAKTAE
                     GRVGFEFEARKPKAEKPAAVAPVPAPAKATRKKRAPAAATAAPARKATAPVKSARTAP
                     AAKPAPRTKRKAA"
BASE COUNT         1818 a         2935 c         2935 g         1755 t
ORIGIN      
        1 gatcttcatg accttggctt gaaccggatc cggcggctca ggattcagct tggtctggat
       61 ccacatcgtc acagccatca ggatcggcag gatgaaccag ggatcggggg ccgcgagatc
      121 ttctatccac agcatgaacg gcgcgtgccg caattcaacg ctggccaaca aagcccaata
      181 aagagcaata aaaacaggta tttgcacaag tatcggcatg cagccgccaa gcggattgat
      241 cttctccgtc ttgtagagct ccatcatcgc ctgctgcatg cgctgccggt cgtcgccgta
      301 ctgttctttc aaccgctgca gctttggggc cagcacccgc atcttggcca tggagcgata
      361 gctcgctgca gacagcggat agaacaacag cttgatgatg accgtgagca gtatgatcgc
      421 gaccccccaa ttgcccgtcc aatcgtagag ccgggccagc acccagaaca gcggcactgc
      481 gatcacggtc agccagccat agtcgatggt gagatccagg cccggggcga ttttcgccag
      541 attctcctgc tcctggggcc cggcataaag cggcaccgcc agcgttgcgc tggcgccggc
      601 gcccagcgtg ccgtcgggaa cgatgacacc cgcggcatac aggttttcat ccagcttgcg
      661 tgtatagaac tcgcgcggcg tgccgtcctt cggcagccag gcgctgaaaa aataatgctg
      721 gagcaccgca atccagccat cgttgctggt ttttggatac ggcgccttgt tcttggcgac
      781 gtcttcgaac gcgagcttct gaaacttttc cttttcggta tagaccgcca tcccggtgaa
      841 ggtcggcagc atcgccgagt ccccgaccgg cggcttgccg tcgcgcacga tctggaaata
      901 cgcatagggc tgcacgaccg ccgcgccctt gttggtaatt tcaaaactga agtcgatcag
      961 gtagcttccc cggcggaagc gatagacctt gtccacctgc acgccatcgg ccgggggcgc
     1021 tctcagccgt acttccaccg tatcctgtcc gtcggcgagc ttgtggctgg tcgaggttgc
     1081 ctggtacgtc gtccggtggt tgggcaaccc gttgccgatc aacccggact gcgcgacata
     1141 ggtgcgctcc ggcgagatct cgaacaggac gaaatccttg ttcctgtcga ggatgtcacg
     1201 gtgtttcttg aactgcagat ggcgtaaatc gccgccctcg atcccgatcc gcgcgatcag
     1261 aaggtccgtt tccacctgta tcgtggctcc cgccgccggg acgctccgtg ccgcctcggg
     1321 cacggtcgcc tgtggcacct tgagcttgtc acccgggacc ggcacctgcg ctgaaggaga
     1381 tggcgctttt gcagccttgt cgccctggac gaccgctggc tgcgccggct gttgatcgcg
     1441 ctgccaggcg tccagcagca ggaacaccga gaacgagaaa acaaaaaaaa gaatcagccg
     1501 ttgcgaatcc ataacctttt ttaccgggaa ggcccgccgc cctgtccgtg tggccgggcg
     1561 caggactctt ccagaagtct ggctatttcc gcgcccgcct ccacattgaa ccccggagtc
     1621 gggcaacggc gcagttccac cacgacgtcc actccttcca gacggtggcg cagcctgcgg
     1681 aaaatttccc gtaccaggcg cttggcacgg ttgcgatcca ccgctcgcgg cgcaacccgc
     1741 ttgctggcaa tgatgcccat gcgcgctaga gacaggccgt taggtctgac gaacaaggtg
     1801 aaatggcggc gccggccggc cgcgccggat gccagggctt ccgtgaactg ctcgcggcgc
     1861 agcagccgtg cactgcgcgg cagcgtggcc ggggctcctg cgctcagacg ctcagccgtg
     1921 cgcgtccttt ggcacggcgg gcgcgcagca ccgcccgccc gccgcgggtc cgcttgcgca
     1981 cgagaaagcc atgagtgcgc ttgcggcgcg tcttcgatgg ctggtaggtc cgtttcatcc
     2041 tggtctgtcc gcctgctgaa aagcgcgcta ttacaacctt taactctcgg tcatgtcaac
     2101 cttttcttga gtgcggaaaa cgtcccgcct gcgtccggtt tctgtggata accgctcccg
     2161 aaaaggataa aatctcgtgc tgaaaactct ccttcgccag ggtccgaatt ccaactccaa
     2221 cgcgacgccg atgaacagct tctggcgctt ctgtctggcc cgcttcgaga aggagctgcc
     2281 accgcagcaa taccaaacct ggatcaagcc gctgcgtttc cacgccgacg gcgaccggct
     2341 gaccctggtt gctccgaacc gcttcgtgct gcaatggatc cgcgacaagt tcctgccgcg
     2401 cattgaattg ctcgcccggg agcagctcaa gcgcaatatc gaagtgctgc tgaccctctc
     2461 cgaaaaagag catgccgcaa cccctcccgc gctcccgccg ctgcaggcgc caaaggaccg
     2521 cggggcgaag cccagcagcc gcgaggtgtc ccgtctcaac cctgcctttg ccttcgatac
     2581 ctttgttacc ggcaaggcca acgaactggc gcgggccgca gcggcccagg ttgcggagcg
     2641 gcccggcgcg gcttacaacc cactcttcat ttatggcgga gtgggcctgg gcaagaccca
     2701 tctgatacag gcgatcggga accgattgcg cgagcaggcc ccggaaacca agatccgcta
     2761 cattcacgcc gaacaatacg tgtccgatgt ggtgcgtgcc taccagcaca aggcattcga
     2821 cgacttcaaa cgctactacc attccctgga cctgctgctg atcgacgaca tccagttctt
     2881 cagcggcaag agccgaaccc aggaagaatt cttttacgcc ttcaatgcgc tgatcgaggc
     2941 gcacaagcag gtggtcatca cctgcgacac ctatcccaag gaaatcgcgg gcatcgagaa
     3001 ccgcatcata tcccgcttcg gctggggtct caccgtggcc gtcgagcctc ccgaactgga
     3061 aatgcgcgtc gcgatcttgc tcaagaaggc ggaggtcgag ggcgttcggc tggatgaagc
     3121 cgtggctttt ttcctcgcca agcatatcca gtccaatgtg cgcgagctgg aaggcgggct
     3181 caaacgggtt cttgcctatg cgcgtttttc cggccgcgat ctgacggccg agttgtgccg
     3241 cgaggccttg cgcgacctgc ttgccatcca gcaccgccag gtgtccattg aaaacatcca
     3301 gcgcaccgtc gccgactatt acaagatcaa ggtctccgag atgtactcga aaaagcgcag
     3361 ccgcaacgtc gcccgcccgc gccagatggc catggcgctc gccaaggaac tgacccagtt
     3421 gagcctgccg gacatcggtg atgccttcgg cggccgtgat catacgaccg tgctgcacgc
     3481 ctgccgcaag atcgcactgc tcaaatccac caacagcgag ctcacccggg atttcaactc
     3541 gctgctcaac gtcctgagaa gctgattgcc tctgtgcgct ttgtggataa ccagaagctt
     3601 ttctccaacg ggattttcca tccacaaatt ccccacaact tctaccggct tctgcacagg
     3661 ttcttcctga ccgcttccgc gcgtgcacac tggatcgagg cgacttgccc acaggaaaga
     3721 cgaacactac tagtcctatt acctttaatt agaaaagtaa acctatgaag ctcgtgcaac
     3781 tcgaacgtga tgcgttgctg agacccttgc aggcggtcac cggcatcgtg gagaaacgtc
     3841 acacgctgcc gatactttcg aatgtcctga tcgagcgaaa gaagaatcgc ctgttgctgg
     3901 tggcgaccga cctggagatc caggtggcaa ccagcacgga gaccggcaag gaagccgacg
     3961 gggacacgag cctcaccgtg tctgcccgca agctgcagga catactgcgc gcgctgcccg
     4021 aagccaccga agcgaccctg gacgtacaga acaaccgtct ccaggtgcgc gcgggcaaga
     4081 gccgcttcaa tctgcagacg ctgccatcat cggattttcc ggccatggcg gatccgggcg
     4141 cggcgcaagc caaggtttcg atgccgcaga aggcgttgag ggaattgctc ctgctggtgc
     4201 agttcgccat ggcgcagcag gacatacgtt actacctgaa cgggctgctg ctggtgctcg
     4261 acggcgggct ggtcagggta gtcgccacgg acggccaccg gttgagctat gccgcgcggc
     4321 cgctgggaca acagcaggag aagcgcgaag tgattctgcc gcgcaaggcg gttctggagt
     4381 tggggcgctt gctgggggac ggggaggagc cggtcagcat cgaaatcttc gcgggcctgg
     4441 tgcgattttc cttcggtaac gtcgtgctca cgaccaagat catcgacggc aagtttcccg
     4501 attacacccg cgtggtgccg acgaactatc agaagcggtt ctcgatcaag cgccaggagt
     4561 tgctccagtc gctgcagcgc gcggcgatcc tgtccaacga aaaattccgc ggcgtgcgct
     4621 ggatggtggc gaccaacagc ctgcgcatct cctgcaccaa caacgagcag gaggaagcgc
     4681 aggaagagct ggaaatcact tacggagagg aagcgctcga tatcggcttc aatatcactt
     4741 acctgctgga cgtgttgaac aacgtgcaca gcgacaatgt cgaatgctcg ttcggggacg
     4801 ccaacagcag catgctggtc accgtgccgg acatccagga gttccgctac gtagtcatgc
     4861 cgatgaggat ctagaaggcg gggaatgacg aaagaggcga tcgagaggga gtcgctgcgc
     4921 tcggcggacg actacgattc cagcagcatc aaggtcctga aaggcctgga ggcggtgcgc
     4981 aagcgtccgg gcatgtatat cggcgacacc ggcgacggca ccgggctgca ccacatggtg
     5041 ttcgaggtgg tcgacaacgc ggtggacgag gcgctcgccg gcttctgtac cgagataacc
     5101 gtcaccattc ataccgataa ctcgatcagc gtcgtcgaca acgggcgcgg cataccgacc
     5161 gacttgcacc cggaggacga ggcgggccgc tcgagcgcgg aagtcgtgat gaccgagctt
     5221 cacgctggcg gcaagttcga caacaattcc tacaaggtgg ccggcggtct gcatggggtg
     5281 ggagtgtcgg tggtgaatgc gctgtcggaa tggttgcgcc tgtcgatacg ccgcgacggc
     5341 cagctccacc agatggaatt tcgcatgggg gagacggtcg cgccgctcaa ggcgaccggc
     5401 aagagcgagc gtcgcggaac cgaggtgcat ttcctgccga gcaaggaagt attcggccgc
     5461 atcgagttcc actacgagat actggctaaa cggctgcgcg agctggcgtt cctcaacagc
     5521 ggcctggaca tcacgctggt cgaccagcgc agcggcaagg aggaaaaatt cctgttcacc
     5581 ggcggggtca agggcttcgt cgagtacatg aaccgcagca agaacgtgct gcatccgaac
     5641 gtgttctacg ccagggccga gaaggatggc atcgtcgtgg aagtggcgat gcagtggaac
     5701 gactcgtacc aggaatccat gcagtgcttc accaacaata tccctcagcg agatggcgga
     5761 acgcacctga ccgggctgcg cgcggccatg acgcgcacgt tgaaccagta catcgagacc
     5821 agcaagctcg cggagaaagc caaggtcgag accgcgggcg acgacatgaa ggaagggctg
     5881 accgccgtgc tgtcggtgaa ggtgccggag cccaaatttt cttcccaaac caaggacaag
     5941 ctggtttctt ccgaggtgcg gccggtggtg gacgagattg tcgcccagaa actgcacgag
     6001 ttcctgctcg agaggccggg cgacgcgcgc atcatctgca gcaagatcat cgacgcggcg
     6061 cgcgcgcggg aagcggcgcg caaggcgcgc gagctcacgc gccgcaaggg cgtgctcgat
     6121 tccttcgggc tgtcgggcaa gctcgccgac tgtcaggaac gcgacccggc ccagtgcgag
     6181 ctctatatcg tggagggcga ttccgccggc ggctcggcca aacagggccg cgatcgcaaa
     6241 ttccaggcga tccttccgct caagggcaag attctcaacg tcgagagggc gcggctggac
     6301 aagctcctgt cgtcgcagga gatcatcacg ttgatcagcg tgctcggcac cggtatcggc
     6361 aaggaggatt acgcgccgga gaaactgcgc taccaccgca tcatcatcat gacggatgcg
     6421 gacgtggacg gttcgcatat ccgcacgctg ttgcttactt tcttctatcg gcagatgccg
     6481 gaattgatcg agcgcggcca cgtctatatc gcccagccgc cgctgtacaa agccaagcat
     6541 ggcaagaccg agcgttatct caaggatgag cacgaactga agcagttcct gctcaagctc
     6601 gcgctgtcgg aggccgagct ctttacggcg gtcaacgggc agacgctcag caaggacgca
     6661 ctggagatgg tggccaagga atatctgctc gccgaggcgg tgatcgagcg ggttagccgc
     6721 ctggtcgacg aggacgtgct gcacgcgctg ctgcgccagc ccgtgcagct cgatctctcc
     6781 gacgagaaag cggcgcgcaa cagcgcgcag gcgctggctg cgctgctcgc gcccatgggc
     6841 atgatggtcg aggcgaagta cgacgagaag gccgagcgct acgcgctggt cgtgaaccgc
     6901 acgcaacacg gcatcgtgcg ccactgctac atagacgccg acttcgtgca gggcggcgat
     6961 tacgaccaga ttcagcgcac ggccaaggtc ctggaaggcc tgcttgcgga aggcgcttac
     7021 gtcaagcgcg gcgagcagca gcaaccggtc agggaattcc gcgaggccat cgaatggctg
     7081 ctgggcgaag tgcagcgcgg ggccagcatc cagcgctaca agggtctggg cgagatgaat
     7141 cccgagcagt tgtgggagac caccatgaat cccaagacgc ggctgctgct gaaaacgcag
     7201 atcgaggatg ccatcagcgc ggaggagatt ttctccaccc tgatggggga ccaggtcgag
     7261 ccgcgccgca atttcatcga ggccaacgcg ctcggcgtgc gcaacctcga catctgaccg
     7321 tctccgctcc ctgcaggaag cctgagaaac gtccctaggc cgctttgcgt ttggttctag
     7381 gtgcgggttt ggctgctggc gccgtgcgcg cggacttgac cggcgctgtt gccttcctgg
     7441 cgggcgccgc ggttgcggcg gcaggtgcgc gcttcttgcg tgttgctttt gccggagccg
     7501 ggacgggcgc aaccgcggcc ggcttttcgg ccttcggttt gcgtgcctcg aattcgaaac
     7561 cgaccctgcc ctcggccgtc ttcgccaggt aggccttgaa cggccgcccc ttctttgaga
     7621 tgaagcgcgt aaagaggtcg gtgcgcccgg tggtcagtaa tttctgcatc tgatcgcgct
     7681 cgatcggttg ctgaagaatc atcttgcccg agcggaaatc gcaggcgcgc tccgcgccga
     7741 ctgctttctc gcagacataa acggtgccgt actcgaacac gcgcgcgccg cacttggggc
     7801 agggacccag gctatcctgg ttgctgaagt cggggggatt ggccgcttcc tcctcgagcc
     7861 gcacctgtcc gaagtcgaac tcgatcttga gatcgctgga gagccggatg cctgctgaaa
     7921 acggcttgcc catgcggctg cggaaaccgt tgagcggacc gatgaaacgc ttctggatca
     7981 actcggcgat ttcctccggc gcccattcgc ggccggagag gacgacccac agcgaaaaat
     8041 cgcatttctg gcactggaac ttgcggtagt tttcctgcac catgccgccg cacttgggac
     8101 agggcgcttc cacggtcgcg taggcttcgt cgggaatgtc gccgttcctc acgcgctcca
     8161 cgagatcgcg ggtcacctgc tcgatgtggt ccatgaacac gtcgcgcttc aactcgccgg
     8221 cttccatctg cttcaacttg tgttcccagt cgcccgtcag ttccggcgag gtgatctcgt
     8281 tgacaccgaa gtgatgcagg gcgaggagca gccggaacgc cttccaggtg ggcttcagtt
     8341 cgcggccttc gcggtggacg taagcctccc ggatcagacc ctcgatgacg gcggcgcggg
     8401 tggccggcgt gccgagacct ttcgcctcca tcgcggcgcg cagctcgtca tcctccacca
     8461 gtttgccggc gccttccatc gcggagagca gtgttgcctc cgagtagcgc gccggcggcc
     8521 tggtctggtt ggcgatggcg ttgatctcga gcacctcgac cttctccccg gcagtgatgg
     8581 gcggcagcgt ctgggattct tcctgtgccg agcggccgta gaccgcgagc cagccggggg
     8641 tttgcaggat gcggccctcg gtcttgaagt gatgctctcc gacctgggtg atgcgcgttg
     8701 tctgcaggta ctccgctgcc gggtggaaca cggcgagaaa acgccgcacc acgagatcgt
     8761 agagcttgtg ctcgggttcg ctcagatgcc ggggcgcttg cagcgtgggg atgatcgcga
     8821 agtgatcgct gattttcgag ttgtcgaaca cgcgcttgtt gggccggacc cagccattct
     8881 tcaaaacgga tttggcaaac ggggcgtatt cccgctgccc gcccagcatg tccatggcct
     8941 tcttcacggt gccgatgtag tcctcgggca gcgcgcgcga gtcggtccgc ggataggtca
     9001 ggaccttgtg tttttcgtag agcgcctgcg ccagccccag cgtgttccgc gccgaaaagc
     9061 cgaagcggcc gttggcctcg cgctgcaggc tcgtcagatc gaacagcagc ggcgagatct
     9121 gcgtggcggg cttggtttcc tcgtcgacgg tgccggtctt gccgcggcag aaggcggtga
     9181 tggcctcggc ctccgccgcg ctccactggc gctcggccct gcgctcggca tcgtcatcct
     9241 tcttgaaaac gggatcgaac cagcgtccgg ggtactcccc cgccctggcg ccgaatttgg
     9301 catgcacctc ccagtaatcg cgcgacctga aagcccgaat gcgttcctcg cgctcgacca
     9361 ggatggcgag cgtcggcgtc tgcacgcgcc cgacggtggt gagatagaat ccgccctcct
     9421 tcgagttgaa agcggtcatc gcg
//
LOCUS       DBNGSG010000158         5415 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2769904, whole genome shotgun sequence.
ACCESSION   DBNGSG010000158 DBNGSG010000000
VERSION     DBNGSG010000158.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5415)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5415)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5415
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2769904"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="AC158Q_04125"
     CDS             <1..377
                     /locus_tag="AC158Q_04125"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3338989.1"
                     /translation="RLSWDVAELPYVEALGPAAVFDIQRIVLEAIANALNHSAAPQVR
                     IAVHARGLEGVEINIEDDGKGFDPVAPGPGMGQANMRARANRLGAGLDIISKPGHGTK
                     VRLTVPLQITQAVGRPAGNPPA"
     gene            complement(430..1128)
                     /locus_tag="AC158Q_04130"
     CDS             complement(430..1128)
                     /locus_tag="AC158Q_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006163575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3338990.1"
                     /translation="MAEQPGMTVRVMIVEDDLGTLERFAGAISRDARTSVAGKARDGR
                     QAIARLPDISPDVLLVDLGLPDMHGTEIIRAASRLLPECDVMVITAFGDERSVVASIE
                     AGATGYVLKDCSDEDLVARVLELRAGGSPMSPGIARIVLTRMRGGIGAASRDGEAAAG
                     AGGSVKSALTLREADVLRLLSRGYTYAEIGEKLGISVHTVTSHIKNSYRKLAVHTAAE
                     AVTRAAGLHLLDRN"
     gene            1459..2418
                     /locus_tag="AC158Q_04135"
     CDS             1459..2418
                     /locus_tag="AC158Q_04135"
                     /inference="COORDINATES: protein motif:HMM:NF028121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HIR3338991.1"
                     /translation="MFHAALASAGITRITFLAKGYALVVALTVFLSTSGCSVRNFAVN
                     SMGDALAASGSGFGSDDDPELISAAAPFSLKLMESVLAESPGHRGLLLAATQGFVQYA
                     YAFVEMRADEIEDRDLQAAYAQRERARRLYLRARNYGLRGLESAHPGIGRLLKADPVS
                     AVARATEKDTALLYWTGVAWAAAISLSKDDPFLVADLPVAEALVRRALALEETYDHGT
                     IHVFLISYEMSRAGLSADAPARARRHFERAVDLTGGLHAAPFVTLAESVAIAERNRPE
                     FEQLLRRALALDTGARAEWRLVNTVMQRRARWLLARTDKLFAD"
     gene            2432..3454
                     /gene="dctP"
                     /locus_tag="AC158Q_04140"
     CDS             2432..3454
                     /gene="dctP"
                     /locus_tag="AC158Q_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HIR3338992.1"
                     /translation="MGTIKHKSLPARLAAAALAFAACNVLAADLTLKIATVAPKGSVY
                     HRVLQDMGEKWRAAEGPNARFIVYTDSAQGPEAETVRRMRIGQLQASMLSIVGLSEID
                     DSVSALQKIPLMFRSWDEVDYVRERLRPDLEQRLRAKGYVVLFWGEGGFVQFFSKSPY
                     TMPAQYKASRIFAWAGDNAQVDLMKSLGYRPVVLELSDILPSLQTGMIDTVPAAPLWA
                     LVGQFDRTASHMLRINWVPIVGAVVMSAKSWDSLSPAGRAALTSAAAEAGDALRAQRG
                     TRDDEIIKAMENRGLKVLPMTAETERAWRDLAQASWPKVRGTMVPAEMFDRVEQLLAE
                     YRGTKR"
     gene            3499..5310
                     /locus_tag="AC158Q_04145"
     CDS             3499..5310
                     /locus_tag="AC158Q_04145"
                     /inference="COORDINATES: protein motif:HMM:NF018513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR3338993.1"
                     /translation="MPSGAKAPDFLVLAENACLSLVFAGLVLLPLAEIFLRAVAGLGF
                     QGSSSLVQHLTFAVGILGAAVAARESRLLGFSAATLLEGAAARIAKLLGNAIAAAVSA
                     LLCLASVQFVESERAAGKILAHGIPVWVVESLLPLGFALITLRLIARSADRIGDRVNA
                     GMLAAAVIAVFVALPLQPRNLAAPGLAGLLAAMLLGAPLFAVIGGAALLLLWEAGVPI
                     ASVAVNHYGLTSNPSLPAIPLFTLAGYFLAESGSPRRLIELFDALFGRHRGGAAVVTV
                     LACTFFTSFTGASGVAILAIGGLAMPLLLAAGYPQRPALGLVTGGGLPGVLLFPALPL
                     LLYAIVARTSIESMFVGGFLPTLLMLGIALWWGIRQAPRQAGPARPFDRERALHALWS
                     AKWDISLPLVPLTILLGGLATPVEAAAATALYAFLIAAALHRDIHPLRDAPRIMTECG
                     LLVGGILLILGVSLGLTNYLVDAQVPERAVEWVSKHIQSPWAFLLALNGFLLLVGCVM
                     DIFSAIVVITPLIVPIGLAFGVHPVHLGIIFLANLEIGYLTPPIGLNLFFSSYRFGRP
                     IPEVCRAVTPLFLALCAGVLAITYLPWLSTGLLGLVR"
BASE COUNT          841 a         1881 c         1760 g          933 t
ORIGIN      
        1 tgcggctgtc ctgggacgtc gcggaactgc cgtacgtgga agcgctcggg ccagccgcag
       61 tgttcgatat ccagcgcatc gtgctcgagg cgatcgcaaa cgcgctcaat cactcggccg
      121 cgccccaggt ccggatagcc gttcacgcca ggggcctgga aggggtggag atcaacatcg
      181 aggacgacgg caagggtttc gaccccgttg cgccggggcc cgggatgggg caggcaaaca
      241 tgcgcgcacg ggccaacagg ctgggcgccg gactcgacat catctcgaaa ccgggacacg
      301 gcacgaaggt tcgtctgacc gtgcccctgc aaatcaccca agcagtcggc cggccggccg
      361 gaaacccgcc cgcgtagtcc ttgggtgcaa tccccgccga tcggctggct cgccatgcac
      421 ggcgccgagc tagttgcgat cgagcagatg gaggcccgcg gcacgggtta cggcctcggc
      481 agccgtatga acggcaagct tgcggtagct gttcttgatg tggctggtga ccgtatggac
      541 cgagatgccg agcttttcgc cgatctcggc gtaggtgtag ccgcgcgaca gcaatcgcag
      601 gacgtcagcc tcgcgcaacg tcagtgccga cttcacgctc ccgcctgcgc cggcggccgc
      661 ttccccgtcc cggcttgcgg caccgattcc gcctcgcatc cgcgtaagaa cgatccgcgc
      721 aatgcccggg ctcatcggag agccgccggc tcgaagctcc agcactcgcg caacgagatc
      781 ctcatcgctg cagtccttca acacgtagcc ggtagcgccc gcctcgatgc tcgcgaccac
      841 gctgcgctcg tcgccgaacg ccgtgatgac catgacatcg cattccggga gcaatcgcga
      901 tgcggcccgg atgatttccg tgccgtgcat gtcgggcagg cccaggtcca ccagcagtac
      961 atcgggcgag atatccggca ggcgcgcaat tgcctgccgc ccgtctcggg ctttcccggc
     1021 cacgctggtg cgggcatcgc gcgagatcgc gccggcaaat cgttccagcg tcccgaggtc
     1081 atcctcgacg atcatcacgc gcacggtcat gcccggctgc tcagccatgt gtccgccgtt
     1141 cacgttcgca ctcgatcatc cgcacgctgc aatccggcca ccctgcccgc ctgcgcatcc
     1201 tacccggaac ccgtcttgcg gcggcatccc atgagcatgg gagagcacag ggaataccac
     1261 aatcatggga tatcggaagg cgccgggctt ggccatgatc gtccaccgaa aaccgaccgg
     1321 ccgcaactca agacagtgac ggacaggatc gcaatggttg cggctgcgca gttgtaagtc
     1381 atccgtaatc cacccgtaag ccatccggaa cgcgccggga gcgaccatgg ccgccaacgc
     1441 aagcacagga ggaaagccat gtttcatgcc gcactcgcca gcgcgggaat cacacgcatc
     1501 acgttcctgg caaagggcta cgccctggtc gttgccctga ccgtgtttct ctcgaccagc
     1561 ggctgctccg tgcgtaactt cgccgtgaac agcatgggcg acgcgctcgc ggccagcggc
     1621 tccgggttcg gctccgacga cgaccccgag ctgatcagcg ccgcggcgcc cttcagcctg
     1681 aagctgatgg aaagcgtgct cgcggagagc ccgggccacc gcgggctgtt gctggcggcc
     1741 acgcagggct tcgtccagta cgcctacgcc ttcgtggaaa tgcgcgccga cgaaatcgag
     1801 gaccgggatt tgcaggcggc ctacgcccag cgcgagcggg cgcggcgcct gtacttgcgc
     1861 gcccgcaatt acgggctgcg cggactcgag agcgcgcacc ccggcatcgg ccggttactc
     1921 aaggccgatc ccgtcagcgc agtcgcgcgg gcgacggaaa aggacaccgc gctgctctac
     1981 tggaccgggg tggcctgggc ggcagcgatc tccctgtcga aggacgaccc cttcctggtg
     2041 gccgatctcc cggtcgcgga agcgctcgtg cgcagggcgc tcgcgctgga agaaacttac
     2101 gaccacggca ccattcacgt attcctgatc agctacgaaa tgagccgcgc cggcctgagc
     2161 gccgatgcgc cggcccgggc gcgacggcat ttcgagcgcg cggtcgacct gaccggcgga
     2221 ctgcacgccg cccccttcgt gacgctcgcc gaatcggtgg ccatcgcgga gcgcaaccgc
     2281 ccggaattcg agcaattgct gcggcgtgcc ctggcgctcg acaccggcgc tcgggcggaa
     2341 tggcggctcg taaacacggt gatgcagcgc cgcgcgcgct ggctgctcgc ccgcaccgac
     2401 aaacttttcg ccgactgacg gaggactccg catgggcacc atcaaacaca agtcgctgcc
     2461 ggctcgcctg gccgccgccg cgctcgcatt cgcggcctgc aacgtgctcg ccgccgacct
     2521 gacgctgaaa atcgcaacgg tggcgccgaa aggctcggtc taccaccgcg tgctgcagga
     2581 catgggcgag aagtggcgcg cggcagaggg tcccaatgcc cgctttatcg tctataccga
     2641 cagcgcgcag gggccggaag cggagaccgt caggcgcatg cgcatcgggc agctgcaagc
     2701 ctcgatgctc agcatcgtgg ggttgagcga aatcgacgat tccgtctccg cgctgcaaaa
     2761 aatcccgctg atgttccgtt cctgggacga ggtggactac gtgcgcgagc ggctgcgtcc
     2821 cgatctcgag cagcgcctgc gcgcgaaagg ctacgtggtg ctgttctggg gtgagggcgg
     2881 cttcgtccag ttcttctcca agtcgcctta cacgatgccc gcgcagtaca aggcaagccg
     2941 cattttcgcc tgggcgggag acaacgcgca ggtggatctg atgaaatcac tgggctaccg
     3001 cccggtggtg ctggaactct ccgacatcct gccctcattg cagaccggaa tgatcgacac
     3061 ggtcccggcc gccccgctgt gggcgctggt cgggcaattc gaccgcacgg cttcccacat
     3121 gctgcgcatc aactgggtgc cgatcgtggg ggccgtggtg atgagcgcga aatcctggga
     3181 ctcgctctct cccgcggggc gcgccgcgct gaccagtgcc gccgccgaag cgggcgatgc
     3241 gctgcgcgcc cagcgcggca cccgcgacga tgaaatcatc aaggccatgg agaaccgcgg
     3301 cctcaaggtg ctgcccatga cggcggaaac ggagcgcgcg tggcgggacc tcgcgcaagc
     3361 ctcctggccg aaagtgcgcg ggaccatggt tccggccgag atgttcgatc gcgtcgagca
     3421 gctgctagcc gagtaccgcg ggacgaaacg atgagccact gcgcgtctgg ctcgcgaacg
     3481 gcaccggccg ccctggcgat gccttccggc gcgaaagcgc cggacttcct ggtcctggcc
     3541 gaaaacgcat gtctgtcact cgtcttcgct ggcctcgtgc tgctgccgct cgccgagata
     3601 ttcctgcgcg cggtcgcggg cctgggattc cagggctcct cctccctggt gcagcacctg
     3661 accttcgcgg tcggcattct cggcgctgcc gtcgctgcgc gcgagagccg cttgctgggg
     3721 ttctcggccg cgaccctgct ggaaggcgcc gccgcgcgca tcgcgaaact cctgggcaac
     3781 gcgattgcgg cagccgtgag cgccctgctg tgccttgcca gcgtgcaatt cgtggaatcg
     3841 gagcgtgcgg ccggaaagat actcgcgcat ggcatcccgg tatgggtcgt cgagtccctt
     3901 ctgccgctcg gcttcgcgct catcacgctg cgcctgatcg cgcgctccgc cgaccgcatc
     3961 ggcgaccggg tcaacgcggg catgctcgcc gctgcggtca ttgcggtgtt cgtcgccctg
     4021 cccctgcagc cgcgaaacct ggccgcgccc ggcctggccg gcttgcttgc ggccatgctg
     4081 ctcggcgctc cgctcttcgc cgtgatcggc ggcgccgcgc tgctgctgct atgggaagct
     4141 ggcgtgccca ttgcttcggt cgcggtgaac cactacgggc tcacgtcgaa tccctcgctg
     4201 ccggcgatcc cgctgttcac gctggccggc tatttcctgg ccgagagcgg ctcgcccagg
     4261 cggttgatcg aactgttcga cgccctgttc gggcgacacc gcggcggcgc cgcggtcgtc
     4321 accgtgctcg cctgcacgtt tttcaccagc tttaccggcg catccggcgt ggcgattctc
     4381 gcgatcggcg ggctcgcgat gccgttgctg ctggccgcgg gatacccgca gcgcccggcg
     4441 ctgggactcg tcaccggcgg cggattgccg ggcgtgctgt tgttccccgc gctgccgctt
     4501 ctgctctacg ccatcgtcgc cagaaccagt atcgagtcca tgttcgtcgg cggcttcctg
     4561 ccgacgctgc tcatgctcgg catcgcgttg tggtggggaa tacggcaggc gccccggcag
     4621 gccgggcccg cgcgcccctt cgaccgggaa cgcgcgctgc atgcactgtg gtcagcgaaa
     4681 tgggacatct cgctgccgct ggtcccgctg acaatcctgc tgggcggact ggcgacgccg
     4741 gtcgaagccg ccgcggccac cgcgctctac gccttcctga tcgccgctgc gctgcatcgc
     4801 gacattcatc ccttgcggga cgcgccgcgc atcatgaccg aatgcggact gctggtgggc
     4861 gggatcctgc tgattctcgg cgtctcgctc ggcctcacca attacctggt ggatgcccag
     4921 gtccccgagc gcgccgtgga atgggtgtcg aaacacatcc aatcgccgtg ggcattcctg
     4981 ctcgcgctga acggcttcct gctgctggtc ggctgcgtga tggatatctt ctccgccatc
     5041 gtcgtgatca cgccgctgat cgtgccgatc ggactcgcgt tcggcgtgca cccggtgcat
     5101 ctcggcatca tcttccttgc caacctggag atcggttatc tcacgccgcc gatcgggctc
     5161 aacctgtttt tctcgtcgta ccgcttcggc cggccgattc cggaagtgtg ccgcgcggtc
     5221 acgccgctgt tcctcgcgct gtgcgcgggt gtgctcgcca tcacctacct gccgtggttg
     5281 agcaccgggc tgctcggcct ggtgcgatga tcgcaaatta acggatttga acactcgaag
     5341 gagggacgca atcatggatc agaccgtgca tcgcctgcac taccaggcct acaagcccgc
     5401 cccgttcacg cgggc
//
LOCUS       DBNGSG010000159         4602 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2600103, whole genome shotgun sequence.
ACCESSION   DBNGSG010000159 DBNGSG010000000
VERSION     DBNGSG010000159.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4602)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2600103"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="AC158Q_04150"
     CDS             <1..280
                     /locus_tag="AC158Q_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3338994.1"
                     /translation="LVVVIPLMHRRLIRHSMGQWYLGDIGLPGGLCLGIGALTYLVAP
                     RDVPPLASFLWILVAYAATFLCTALAMPHTRAWLKSLVFAAGDAKVKS"
     gene            428..1477
                     /locus_tag="AC158Q_04155"
     CDS             428..1477
                     /locus_tag="AC158Q_04155"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar O-methyltransferase"
                     /protein_id="HIR3338995.1"
                     /translation="MNPDFTGDAVLSEMVAEVGAASPLYRPSNFWNNLNQANLEMLET
                     HGLDNFKRTVAQNYYNWMVIGLRDNQLRSLVRDWFLHPSMRPFLNSIEVPDILRTTVG
                     LERRIGWREMAVYKIFVGLLWEFTRRHDRTGLSQQLQEPSIGNPIVIRRKSRLISQDL
                     ANSIREFGAIREAHPALGRSRTTIAELGAGYGRLAFVALSAPESRYIVFDIPPALRVS
                     QWYLTQIFGKEKIFPFRRFRRYADIRDELEASRIGFFTPNQIEMFPDRVFDVFVTVST
                     LSEMRADQTRNYLQQMQRTVRDVVYLKQWASWQNVEDGRSFSRADISFSELTCVMDRN
                     DAVQDRFLEQVWKRI"
     gene            1572..2339
                     /gene="rfbF"
                     /locus_tag="AC158Q_04160"
     CDS             1572..2339
                     /gene="rfbF"
                     /locus_tag="AC158Q_04160"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HIR3338996.1"
                     /translation="MKCVILAGGLGTRISEESYLRPKPMIEIGGKPVIWHIMKIYSAH
                     GINDFVVCLGYKGYVIKEYFANYFLHMSDVTLDMANNKMEVHQKGAEPWRVTLVDTGD
                     NTQTGGRLKRVAGYLGSEDFCFTYGDGVADVNIRELIAFHRSQKVKATLTAVQPPGRF
                     GALALEDRLITSFQEKPKGDGGWINGGFFVLSPRVIDYIKDDATVWEKGPLEGLARER
                     SLAAYFHRGFWQPMDTLRDKNHLEELWAGKAPPWKVW"
     gene            2303..3412
                     /gene="rfbG"
                     /locus_tag="AC158Q_04165"
     CDS             2303..3412
                     /gene="rfbG"
                     /locus_tag="AC158Q_04165"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HIR3338997.1"
                     /translation="MGRQGAALESLVISHSFWPGKRVLVTGHTGFKGGWLALWLQRLG
                     AQVMGYSLPAPTQPSLYEAARVSESVQGVIADIRDLARLESMFRDFSPEIVFHLAAQP
                     LVRASYTDPVETYSTNLLGTVHVLEAVRRAKGVRSAVMVTTDKCYENLESARGYRETD
                     RLGGHDPYSNSKACAELAVDSYRRSFFTGAGTAAIASARAGNVIGGGDWAADRLVPDL
                     VRAALAGKPLVLRNPKSTRPWQHVLEPLSGYLMLAQRLCDERSRYAEAWNFGPLEDDA
                     RTVEDIVMLAARGWGDGLKWAVDSAAQPHETRMLKLDCSKAAGAGWQPLLRAEEAVAW
                     TMEWYRAWRDKLDVKALSIEQIGRYSRLMAAGKSA"
     gene            3409..>4602
                     /locus_tag="AC158Q_04170"
     CDS             3409..>4602
                     /locus_tag="AC158Q_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR3338998.1"
                     /translation="MTACRSCGAALAHTFADLGMSPISNAFLKAEDLNRMEPFYPLHA
                     YVCAECFLVQLEEFESPQKIFGDHYAYFSSYSDSWLDHARRYTEQMTARFGLGAQSRV
                     IEIASNDGYLLQYFRQKGVPVLGIEPARNVAEAAVAKGIPTLAKFFGVATAAELVATG
                     ARADLLLGNNVLAHVPDLNDFVAGMKLVLKPDGVLTMEFPHLQHLIEQNQFDTIYHEH
                     FSYFSFLAAERVFARHGLQLFDVEELPTHGGSLRIYGRHAGQAGAPGERVRALAARER
                     ALKFEHLDTYRAYAGRVRETKRRLLEFLISARRAGKTVVGYGAPAKGNTLLNYCGIRS
                     DFIDFTVDRSPHKQNTFLPGTHIPVYAPERIDEVRPDYVLILPWNLRDEIVKQMAHIR
                     SWGGRF"
BASE COUNT          813 a         1430 c         1536 g          823 t
ORIGIN      
        1 cctggttgtc gtcataccgc tcatgcaccg gcgcctgatc cggcacagca tggggcagtg
       61 gtacctcggg gacatcggac tgccgggcgg cctttgcctg ggcatcggcg ctctgacgta
      121 cctggtcgcg ccgcgtgacg ttccgccatt ggcgagcttt ctgtggatcc tcgttgccta
      181 tgcggcgacg ttcctttgca ccgctctcgc catgccgcat acgcgggcgt ggctgaaaag
      241 cctcgtattc gcggctggcg atgcgaaagt gaagtcgtag cggcgggcga ggatggcgcc
      301 ggacgatttc acgggggccg tgctgataac atggcgacgg gtttcaccag gccgtttcac
      361 cgaaacatga aagcggccgc cagggaatct ttcgtgcgcc gcatgcgttt attctcgtga
      421 tccggttatg aatcccgatt tcaccgggga tgccgtgctg tcggaaatgg tcgcggaggt
      481 cggcgccgcg tcgcccttgt accggccgtc gaatttctgg aacaacctca atcaggcaaa
      541 tctggagatg ctggagacgc acggcctcga caatttcaag cgcacggtcg cccagaatta
      601 ttacaactgg atggtgatcg gcctgcggga caaccagttg aggagtctcg tccgggactg
      661 gttcctgcac ccgtcgatgc ggccgttcct caattcgatc gaagtgccgg atattctgcg
      721 gacgacggtc ggcctggagc ggcgtatcgg ttggcgggaa atggccgtct acaagatctt
      781 tgtcggcctg ctctgggaat ttaccaggcg gcacgaccgc accggcctct cgcagcagct
      841 gcaggagcct tccatcggca acccgatcgt cattcgcagg aagtcgcgcc tgatttccca
      901 ggacctggcc aactccataa gggaatttgg cgcgatccgc gaagcgcatc ccgcgctcgg
      961 gcgatcgcgc accacgatcg cggagctcgg cgccggctac gggcggctgg ccttcgtggc
     1021 gctttcggcg cccgaatccc gttacatcgt tttcgacatc cccccggcgc tgcgcgtttc
     1081 ccaatggtac ctgacgcaaa ttttcggaaa ggaaaaaata ttccccttcc ggcgtttccg
     1141 gcgctatgcg gatatacgcg acgagctcga ggcatcccgg atcggatttt tcacgcccaa
     1201 tcagatcgag atgtttccgg accgggtgtt cgacgtgttc gtgacggtga gcacgctgtc
     1261 cgagatgagg gcggatcaga cgcgcaatta cctgcaacaa atgcagagaa cggtgaggga
     1321 tgtcgtgtac ctgaagcaat gggccagctg gcagaacgtc gaagatggca ggtcgttttc
     1381 acgcgccgat atttctttca gcgaattgac ctgcgtgatg gatcgaaacg acgccgtgca
     1441 ggaccgcttc ctggagcagg tctggaagag gatttgatgg gctgccggcg caacgcaaaa
     1501 tatctccttg cgccctgatt ttctgagaaa atacgcccgt ttatccaacg gtcggggata
     1561 gccgagggcc tatgaagtgc gtgattctgg cgggagggct gggcacccgc atcagcgagg
     1621 agtcctacct caggccgaag cccatgatcg agatcggcgg caagccggtc atctggcaca
     1681 tcatgaagat ctactcggcg cacggcatca acgacttcgt cgtctgcctg ggctacaagg
     1741 gttacgtcat caaggagtac ttcgccaact acttcctgca catgtccgac gtcacgctcg
     1801 acatggcgaa taacaagatg gaggtgcacc agaagggcgc cgaaccgtgg cgcgtgacgc
     1861 tggtcgacac gggtgacaac acccagaccg gcgggcgcct taagcgcgtc gccggctatc
     1921 tcggcagcga ggatttctgc ttcacctacg gcgacggcgt cgcggacgtg aacatccgcg
     1981 agctgatcgc tttccaccgg tcgcagaagg tcaaggccac gctgacggcg gtgcagccgc
     2041 ccggacgctt cggtgcgctc gcgctggaag accggctgat cacgagcttc caggagaagc
     2101 cgaagggcga cggcggctgg atcaatggcg ggttcttcgt gctgtctccc cgggtgatcg
     2161 attacatcaa ggacgacgcc accgtctggg aaaaggggcc gctggaagga ctggcgcgcg
     2221 agcgctcgct cgccgcctat ttccaccgcg gtttctggca gccgatggat accctgcggg
     2281 acaagaacca tctcgaggag ctgtgggcag gcaaggcgcc gccctggaaa gtctggtgat
     2341 ctcgcacagc ttctggccgg gcaagcgcgt cctcgtaacc ggccacaccg gcttcaaggg
     2401 cggctggctc gcgctctggc tgcagcgcct gggcgcgcag gtcatgggct actccctgcc
     2461 ggcgccgacg cagcccagcc tgtacgaggc cgcgcgggtt tcggaaagcg tgcagggcgt
     2521 catcgcggac atccgcgacc tggcgcggct cgagtccatg ttccgggatt tttccccgga
     2581 gatcgtgttt cacctcgcgg cgcagccgct ggtgcgcgct tcctacactg atcccgtgga
     2641 gacgtactcg accaacctgc tcggcacggt gcatgtgctg gaggcagtgc ggcgcgcgaa
     2701 gggcgtgcgc agcgcggtga tggtgaccac ggacaagtgc tacgagaacc tggaatccgc
     2761 caggggctac cgcgagaccg accggctcgg cggccacgac ccgtacagca acagcaaggc
     2821 ctgcgcggaa ctggcggtgg attcctaccg gcgatcgttt ttcaccggcg cgggcacggc
     2881 ggcgattgct tcggcgcgcg cgggaaacgt gatcggggga ggggactggg ctgccgaccg
     2941 cctggtgccg gacctggtgc gggccgcgct cgccggcaag ccgctggtgc tgcgcaatcc
     3001 gaaatcgacg cgcccctggc agcacgtgct ggagccgctg tcgggctacc tgatgctggc
     3061 gcagcggctt tgcgatgagc gctcgcgcta tgccgaagcg tggaatttcg ggccgctgga
     3121 ggacgacgcg cggacggtcg aggacatcgt gatgctggcg gcgcgcgggt ggggagatgg
     3181 cctgaaatgg gccgtggatt ccgctgcgca gccgcatgaa acgcgcatgc tcaagctcga
     3241 ctgctccaag gccgcgggcg cgggatggca gccgctgctg cgcgcggagg aggcggtggc
     3301 gtggacgatg gagtggtacc gcgcgtggcg cgacaagctc gacgtgaaag cgctgtcgat
     3361 cgagcagatc gggcgctaca gccggctgat ggccgcaggg aagtccgcat gacggcctgc
     3421 cggtcttgcg gggccgcgtt ggcccacacc ttcgccgacc tcggcatgag cccgatctcg
     3481 aacgcgtttc tcaaggcgga agatctcaac cgcatggaac cgttctatcc gctgcacgcc
     3541 tacgtctgcg cggagtgctt cctggtgcag ctggaggaat tcgagagccc gcagaagata
     3601 ttcggcgacc actacgccta tttctcctcg tactcggact cgtggctcga tcacgcgcgg
     3661 cggtataccg agcagatgac ggcccgcttc ggcctgggcg cgcagagccg ggtgatcgag
     3721 atcgccagca acgacggcta cctgctgcag tacttccggc agaagggcgt gccggtgctc
     3781 ggcatcgagc cggcccggaa cgtggccgaa gccgcggtgg cgaaaggcat acccacgctg
     3841 gcgaaattct tcggggtcgc gaccgcggcg gaactcgtcg cgacgggcgc gcgcgccgac
     3901 ctcctgctcg gcaacaacgt gctcgcccac gtgccggacc tcaatgattt cgtcgccggc
     3961 atgaagctcg tcctgaagcc ggacggcgtg ctgacgatgg agtttcccca cctgcagcac
     4021 ctgatcgagc agaaccagtt cgacaccatc taccacgagc atttctcgta cttctcgttc
     4081 ctcgccgccg agcgggtgtt tgcgcgccac gggctgcagc tcttcgatgt ggaggaactg
     4141 ccgacccatg gcggctcgct gcgcatttat ggccgccacg ccgggcaggc cggcgcgccg
     4201 ggggagcggg tgcgggcgct ggccgcgcgc gagcgcgcgc tgaaattcga gcatctcgat
     4261 acctaccgcg cgtacgccgg gcgggtgcgg gagaccaagc ggcggctgct ggaattcctg
     4321 atctcggcgc ggcgcgccgg caagacggtg gtgggctacg gcgcgccggc caagggcaac
     4381 acgctgctca actactgcgg catccgcagc gacttcatcg actttaccgt ggaccgcagc
     4441 ccgcacaagc agaacacctt cctcccaggc acacacatcc cggtctatgc cccggagcgc
     4501 atagacgagg tcaggccgga ttacgtcctg atcctgccgt ggaacctccg cgacgagatc
     4561 gtgaagcaga tggcccacat ccgttcctgg ggcggcaggt tc
//
LOCUS       DBNGSG010000160         2897 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2600303, whole genome shotgun sequence.
ACCESSION   DBNGSG010000160 DBNGSG010000000
VERSION     DBNGSG010000160.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2600303"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2897)
                     /locus_tag="AC158Q_04175"
     CDS             complement(<1..>2897)
                     /locus_tag="AC158Q_04175"
                     /inference="COORDINATES: protein motif:HMM:NF012901.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HIR3338999.1"
                     /translation="RVIYHRSRLQSTTKGMGQMTAVGLGEQAARGLLEQLGLTHALEI
                     AGINSSNSVTVAGRAAALDALEVAMTQRKTTYKRLDLDYAFHSPAMDPIEAGILEALA
                     DLRPGGWRIPFHSAVTGERLEGARLGADYWWRNIREPVRFEKALKGIQAGGGRIFIEI
                     GPHALLRGYINACLREEGIQGRVLPTLLRDDDSPARLWSALAQAAIAGATIDWTALFP
                     GRGRLVALPNYPWQRENFWLAATPGSSQRLNRKKEHPLLGYRLHESEWTWENQVDTSL
                     YPVLADHVIGDATVMPGSGFAEMSMAAARLWLAEELVEIEQLEIRAPLVLSDAQSKVV
                     RFSIDTPDGGFTIKSRDQMGSEAWTIHATGRILREPKGMLLQQAPLVLPAHPADFSGA
                     DHDQMTAAIGLSYGPAFRAIDAVWVEGGAAYARFRIPEAIEPELEQTHLHPAILDCAF
                     QLIIELLKDEYATQGGAIYVPAKIDALLVRCGSARPHMARATLQRFGPHSVVASFVVF
                     DAAGVPIASIKEARFRSIRVRKNPSDRLRYLDYHGIPKPHPLSPVTAPRRLFEQLQQK
                     FSALAHAAGRDTHKRYTSEIEPLLDGLCGRFALRSLKSLSADGHTLTVADVHACLDAN
                     PGTGPLLARLIGMLEEDHAIEAAGEGWRFMPDEGLPSPEEIWNSLVTDHPDYFSIFHS
                     VGRVGMHLTDLLWGRKTPEQIYPRECTLSALTRRVLVAAGMPAMQQMLHDLVEQTLTH
                     LPAGKRLRIVEIGADTPSFAAQVCKGVDFNRCDYVYTTLAPSIPEECHRLRERFPGVE
                     IRQIDADSDPVAGAPLANEQFQLALIAGDFATERSALLALAHMRRHLAPGAAVLLVEQ
                     HPSRWMDFIFGGQRAWWSEGPGETWTSRHKVASFWRHQLQQLGLHAGTTLELSADASS
                     GPYILLAQATEQANAAPASPKPVTRTWLMLADKEGFSARFATQLARTLGARG"
BASE COUNT          420 a          998 c         1012 g          467 t
ORIGIN      
        1 ccgcgggcgc ccagcgtcct cgcaagctgc gtggcaaacc gggcggagaa tccttccttg
       61 tcggccagca tcagccaggt ccgggtgacc ggcttcgggg aagccggggc ggcgttcgcc
      121 tgttccgtcg cctgcgccag cagaatgtac ggtcccgagc tggcatccgc cgacagctcc
      181 agcgtcgtgc ccgcgtgcaa tccgagttgc tgcagctggt ggcgccagaa cgaggcgacc
      241 ttgtggcgcg aggtccaggt ttcgcccggg ccctcggacc accaggcgcg ctgcccgccg
      301 aagatgaagt ccatccagcg cgacggatgc tgctcgacga gcaggaccgc cgcccccgga
      361 gcgaggtgcc ggcgcatgtg ggcgagcgcg agcaacgcgc tcctctcggt ggcaaaatct
      421 ccggcgatca gcgcgagctg gaattgctcg ttggccaatg gcgcgcccgc caccgggtcg
      481 gaatccgcgt cgatctgccg tatctccacg cccgggaacc gttcccgcag gcggtggcac
      541 tcttccggta tggagggcgc cagcgtcgtg taaacgtaat cgcagcggtt gaaatccacg
      601 cccttgcaga cctgggccgc gaacgacggg gtgtccgcgc cgatctcgac gatccgcagg
      661 cgcttgcccg ccggaaggtg cgtgagcgtc tgctccacca ggtcgtgcag catctgctgc
      721 atcgccggca tgccggcggc gaccagcacg cggcgcgtga gcgcgctcaa cgtgcattcg
      781 cgggggtaga tctgctcggg cgtcttgcgg ccccacagca gatcggtcag gtgcatcccg
      841 acgcgcccga cgctgtggaa gatcgagaag taatccgggt ggtcggtcac caggctgttc
      901 cagatttcct cgggcgaagg caggccttcg tcgggcatga agcgccagcc ctctccggcg
      961 gcctcgatcg catggtcttc ctcgagcatc ccgatcaggc gcgcgagcag cgggccggtg
     1021 cccgggttcg catcgaggca cgcgtgcacg tcggccacgg tgagcgtatg gccgtccgcc
     1081 gagagcgact tgagcgagcg cagggcaaaa cggccgcaca ggccgtccag cagcggctcg
     1141 atttccgacg tgtagcgctt gtgcgtgtcg cgcccggcgg cgtgcgccag ggccgagaat
     1201 ttttgctgca gctgctcgaa caggcgccgc ggcgccgtca ccggggacag cgggtgcggc
     1261 ttgggtatgc cgtggtaatc gaggtagcgc aggcggtcgg aggggttctt gcgcacccgg
     1321 atgctgcgga agcgcgcttc cttgatgctg gcgatgggca cgccggcggc atcgaaaacg
     1381 acgaagctcg ccaccaccga gtgcgggccg aaacgctgca gcgtcgcgcg cgccatgtgc
     1441 ggccgcgcgc tgccgcagcg cacgagcagc gcgtcgatct tggccggcac gtagatcgcg
     1501 ccgccctgcg tggcgtattc gtccttcagc agctcgatga tgagctggaa ggcgcagtcc
     1561 aggatggccg ggtgcaggtg cgtctgctcc agctccggct cgatcgcctc cggaatgcgg
     1621 aaacgcgcgt aggccgcgcc gccttccacc cagacggcgt ctatcgcgcg gaacgcgggc
     1681 ccgtaactca ggccgatcgc cgcggtcatc tggtcgtgat cggccccgct gaagtcggcg
     1741 gggtgcgcgg gcagcaccag cggcgcctgc tgcagcagca tccccttcgg ttcgcgcagg
     1801 atccggcccg tggcgtggat cgtccatgcc tcgctgccca tctggtcgcg gctcttgatg
     1861 gtgaaaccgc cgtccggcgt gtcgatgctg aatcgcacca ccttggactg cgcatcgctc
     1921 agcaccaggg gcgcgcggat ctccagctgc tcgatctcga ccagctcttc cgcgagccag
     1981 aggcgcgcgg ccgccatcga catttccgcg aagccggatc ccggcatcac ggtggcgtcg
     2041 ccgatcacgt ggtccgcgag cacgggatag agcgaggtgt cgacctggtt ctcccaggtc
     2101 cactcgctct cgtgcaggcg gtagcccagc agcggatgct ccttcttccg gttcagccgc
     2161 tggctcgacc cgggcgtggc cgcgagccag aagttctcgc gctgccacgg gtaattgggc
     2221 agcgcaacga gcctgccgcg gccggggaac agcgccgtcc agtcaatcgt ggcgccggca
     2281 atcgcggcct gggcgagcgc gctccacagg cgtgccgggc tgtcgtcgtc gcgcagcagg
     2341 gtgggcagga cccgcccctg gatgccttcc tcgcgcaggc aggcgttgat gtagccgcgc
     2401 agcagcgcgt gcgggccgat ctcgatgaag atcctgccgc cgccggcctg gatgcccttc
     2461 agcgctttct cgaatcgcac cggctcgcgg atgttgcgcc accagtagtc ggcgccgagc
     2521 cgggcgccct cgagccgctc ccccgtcacc gcggaatgga acggaatgcg ccacccgccg
     2581 ggacgcaggt ccgccagcgc ttccaggatg ccggcctcga tcgggtccat cgccgggctg
     2641 tggaacgcgt aatcgagatc gaggcgcttg tacgtggtct tgcgctgggt catcgccacc
     2701 tcgagcgcgt cgagcgcggc agccctgccg gcaacggtca cgctgttcga gctgttgatg
     2761 ccggcgatct ccagcgcgtg cgtgagcccc agttgctcca gcaggccccg cgctgcctgc
     2821 tcgcccagcc cgacggcggt catctgtccc atgcccttgg tcgtgctctg cagacggctg
     2881 cggtgataga tcacgcg
//
LOCUS       DBNGSG010000161         3878 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3449490, whole genome shotgun sequence.
ACCESSION   DBNGSG010000161 DBNGSG010000000
VERSION     DBNGSG010000161.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3449490"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="AC158Q_04180"
     CDS             complement(<1..235)
                     /locus_tag="AC158Q_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR3339000.1"
                     /translation="MAVLVAASPLSGHAQEKALNLYSSRHYETDEALYSSFTRQTGIK
                     INRIEGGEDPLIERIRNEGARSPADVLITVDAGR"
     gene            427..1323
                     /locus_tag="AC158Q_04185"
     CDS             427..1323
                     /locus_tag="AC158Q_04185"
                     /inference="COORDINATES: protein motif:HMM:NF015891.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HIR3339001.1"
                     /translation="MTNRATSLISLRALFTLALLCVAGVAHAQTTVLEVIPLRYRTAQ
                     EVIPIIQPMLSREGSVSGLQGQLIVRATPANLEEVKRILASIDTAPRQLLISVRQDAD
                     ADRSRSGAEVSGSVGGEHGRVTIPGSRDARGGNVVIREGDDRLRVHAAESRGVESDRN
                     TQTVRVLEGREAFVGTGQSVPVRERQVRRVLLNGQWVEQVVESTQYRDVITGFYVLPR
                     LAGDRVTLDVSPQRETLSRQIPGGVNVQGLVTTVSGRLGEWMEIGGISRDASGQQTVL
                     LGRTTTAMRDSRRVLVKVEEVR"
     gene            1467..3434
                     /gene="yoaA"
                     /locus_tag="AC158Q_04190"
     CDS             1467..3434
                     /gene="yoaA"
                     /locus_tag="AC158Q_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair helicase YoaA"
                     /protein_id="HIR3339002.1"
                     /translation="MVDLDKLFSAAGPLARVMKDFRERPFQLEMAHAVADTIGGRGRL
                     VAEAGTGTGKTVAYLVPALLSGGKVILSTGTKTLQDQLFDRDIPAVREALNVPVTVAL
                     LKGRANYVCHYHLERALNDGRLPTREEVRHLAAIASFARSTTTGDKADCVAVPENSGA
                     WIHATSTRENCLGSPCPHYDKCFIMEARRKALAADVVVVNHHLFFADLALRDEGVAEL
                     LPASNTVIFDEAHQLPDTATLFFSESLSTTQLTGLARDARLEGAAAARDYTPLPKAAD
                     ALDKAARDLRLVFRQDSGRLPARALDGDKDFAAGLDAVLAGLDRLAGELKGQAERSEG
                     LQRCWQRALTLAEVLERWRSGVDEARVKWAELFRHSLHLNSTPLSIAEIFAARIGERA
                     RAWVFTSATLSVNRDFGHYCGQMGLADARTASWDSPYDFADQALMYVPERLPEPNTPE
                     HTEAVVDAALPVIEAAGGGAFMLFTSLRAMRNGHERLREALEARGLDYPLLVQGEGSR
                     SEMLVRFRDLGNAVLVGSHSFWEGVDVRGEALSLVVIDKLPFAAPDDPVLAARIDQMN
                     REGRSAFFDYQLPEAVITLKQGAGRLIRDETDRGVLMICDPRLLTRSYGKRILASLPP
                     MRLTRSAKEAAAFFTGRSTTRPSARPAKTRK"
     gene            complement(3379..3780)
                     /locus_tag="AC158Q_04195"
     CDS             complement(3379..3780)
                     /locus_tag="AC158Q_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HIR3339003.1"
                     /translation="MKRLREWARQLKHEVVALWFCTRHPRTPFMAKALAAALVAYAFS
                     PIDLIPDFIPVLGYLDDLILLPIGIWLVLKLVPADVLAECRDQATRWLDEKNPEPRSR
                     LAAVLIVTLWLAALIFVSWLAWRWAESWIAL"
BASE COUNT          670 a         1189 c         1356 g          663 t
ORIGIN      
        1 ggcgaccagc gtccacggta atcagcacgt cggccgggct gcgcgcacct tcgttgcgga
       61 tgcgctcgat caagggatcc tcgccgccct cgatgcggtt gatcttgatg ccggtttgcc
      121 gggtgaagct ggagtacagc gcctcatcgg tttcgtaatg gcgggaggaa tagaggttga
      181 gagccttttc ctgtgcatga ccgcttagcg gtgatgcagc cactagtacc gccatcagga
      241 agcagcggct tgcggcattc aacgctttga acataaagga gaactcctgt tagtgcggtt
      301 gggattcgtt gccgggaaga atactaacaa gaatggttct caaatgcaat attaatgata
      361 actattctta ttattgcttg cctgggctgg cgtaaactga tgtcatcagc ctgcgttgat
      421 gacgtcatga cgaaccgggc cacatcgctc atatcattgc gtgcgttgtt cacgctcgcg
      481 ctgttgtgcg tcgcgggcgt ggctcacgcg cagaccaccg tgctcgaagt catcccgttg
      541 cgctatcgca cggcccagga agtcatcccg atcatccagc cgatgctctc ccgcgagggc
      601 agcgtgagcg gactgcaggg gcagctcatc gtgcgcgcca cgcccgccaa tctggaggag
      661 gtgaagcgca ttctggccag catcgacaca gccccgcggc agctgctgat ctcggtgcgg
      721 caggatgcgg acgccgaccg cagccgcagc ggcgccgagg tatcgggcag cgtgggcggg
      781 gagcatggcc gggtgactat ccccggcagc cgcgatgcac gcggcggtaa tgtcgtcatt
      841 cgcgagggcg acgaccggct tcgcgtgcac gccgcggaaa gccgcggcgt ggaaagcgat
      901 cgcaacacgc agacggtaag ggtgttggaa gggcgcgagg ccttcgtggg caccgggcag
      961 tccgtgcccg tgcgcgagcg ccaggtccgc cgggttttgc tcaacggcca gtgggtggaa
     1021 caggtcgtgg agagcacgca ataccgcgac gtcatcaccg ggttctatgt cctgccgcga
     1081 ctggccgggg atcgcgtgac gctcgatgtc agcccgcagc gcgaaacgct ttcgcggcag
     1141 atacccggag gcgtgaacgt acaaggcctg gtgacgaccg tctccgggcg gctgggtgag
     1201 tggatggaga tcggcggcat cagccgggac gccagcgggc agcagacggt gctgctgggc
     1261 cgtaccacga cggcgatgcg cgacagccgg cgcgtgctgg tgaaggtcga ggaagtgcgc
     1321 tgagcgctgc gggcagtaaa cggtcgactg gctggctcct ctccggtaat cgagaaagag
     1381 cgcaacgtcg gccggcagct cgggtggggc ggggcgctcg cggtattcaa acagtcgtcc
     1441 cctgatcaac tgttaccatg cgccttgtgg ttgatctgga taagctgttc tccgctgcgg
     1501 gcccgctggc gcgggtcatg aaggattttc gcgagcggcc attccagctg gagatggcgc
     1561 acgcggtggc cgatacgata ggcgggaggg gcaggctggt cgcagaggcc ggcaccggaa
     1621 cgggaaaaac cgtcgcttat ctggttccgg cgctgctatc cggcggcaag gtcatactgt
     1681 ctaccggcac caagacgctg caggaccagc ttttcgaccg tgacatcccc gcggtgcgcg
     1741 aggcgctcaa tgtcccggtc acggtggctt tgctgaaggg ccgggccaat tacgtctgtc
     1801 attatcatct cgagcgcgcg ctgaacgacg ggcgcctgcc cactcgggaa gaggtgcggc
     1861 acctcgccgc gatcgcgagc ttcgcccgca gcacgactac cggggacaag gccgattgcg
     1921 tcgccgtgcc ggagaactcc ggcgcctgga ttcacgccac ttccacccgg gagaactgcc
     1981 tcggttcccc gtgccctcat tacgacaagt gcttcattat ggaagcgcgc cgcaaggccc
     2041 tggcggccga cgtggtggtg gtgaaccatc acctgttttt tgccgacctg gcgctgcgcg
     2101 acgaaggggt ggccgaactg cttccggcca gcaacacggt gatcttcgac gaagcgcacc
     2161 agttgccgga taccgcaacc ctgttcttca gcgagagcct ctcgaccacg cagttgaccg
     2221 ggctcgcgcg cgacgcgcgg ctggagggag cggccgcggc ccgggattac accccgttgc
     2281 cgaaggcggc ggatgcgctc gacaaggccg cacgcgacct gaggctcgtc ttcaggcagg
     2341 acagcggccg gctgcccgcc cgcgcgctgg acggtgacaa ggatttcgca gcggggctcg
     2401 acgcggttct ggcagggctc gaccgtctcg cgggggaact gaaagggcag gccgagcgca
     2461 gcgagggcct ccagcgttgc tggcagcgcg cgctgaccct ggccgaggtg ctcgagcgct
     2521 ggcgcagcgg cgtggacgag gcgcgcgtga aatgggccga gctttttcgt cactcgctgc
     2581 atctcaactc gacgccgctt tcgattgcgg agatcttcgc ggcccggatc ggagaacgcg
     2641 cgcgggcgtg ggtattcacc tcggcgacgc tctcggtcaa ccgagacttc ggccactact
     2701 gtggccagat gggactcgcg gatgcacgca ccgcgagctg ggacagcccg tatgatttcg
     2761 cggaccaggc cctgatgtat gtcccggagc ggttgccgga gcccaacacg ccggagcata
     2821 cggaagccgt ggtggacgca gcgctgccgg tcatcgaggc ggcgggcggc ggggcgttca
     2881 tgctgtttac cagcctgcgg gcgatgcgca acggtcacga gaggctccgg gaggccctgg
     2941 aggcgcgcgg gctggattat ccgctgctcg tccaggggga aggctcgcgc tccgagatgc
     3001 tggtgcgctt ccgcgatctc ggcaacgcgg tgctggtagg cagccattcg ttctgggagg
     3061 gggtggatgt gcgcggcgag gcgctgtcgc tcgtggtgat cgacaagctg ccgttcgccg
     3121 cgcccgacga cccggtgctg gccgcgcgca tcgatcagat gaaccgcgag gggcgcagcg
     3181 cttttttcga ctaccagttg cccgaggcgg tgatcaccct gaagcagggc gcgggccggc
     3241 tgatacggga cgagaccgac cgcggcgtgc tcatgatctg cgatccgcgg ctgctgacgc
     3301 gcagctacgg caagcgcatc ctggcgagct tgccgcccat gaggctcacc cgcagcgcca
     3361 aggaagcggc ggcattcttt acagggcgat ccacgactcg gcccagcgcc aggccagcca
     3421 agacacgaaa atgagagccg ccagccacaa agtgacgata aggacagcgg caagacggct
     3481 ccgcggttcc ggatttttct cgtcgagcca gcgcgtggct tgatcgcggc attccgcgag
     3541 cacgtcagca ggcacgagct tcagcaccag ccagatgccg atcggcagca ggatcagatc
     3601 gtccaggtaa cccagcacgg ggatgaaatc gggtatgagg tcgatcgggc tgaaggcgta
     3661 ggccacgagc gccgcggcca gcgccttggc catgaacggc gtgcgcgggt ggcgggtgca
     3721 gaaccacagc gccacaactt cgtgcttcag ctgccgcgcc cactccctga ggcgcttcat
     3781 ggcgccgttc cgtctctcgt tcctcgtcgt tcgtgcctcg tttcttcgcc gggaattcgc
     3841 ccggcgggcg gggttgaacg agtgacgagc aacgggag
//
LOCUS       DBNGSG010000162         8802 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2770980, whole genome shotgun sequence.
ACCESSION   DBNGSG010000162 DBNGSG010000000
VERSION     DBNGSG010000162.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 8802)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8802)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8802
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2770980"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="AC158Q_04200"
     CDS             <1..629
                     /locus_tag="AC158Q_04200"
                     /inference="COORDINATES: protein motif:HMM:NF014874.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate ligase domain-containing protein"
                     /protein_id="HIR3339004.1"
                     /translation="GADFGVTLQDKQWQYWGPRGKRNALPHPALRGPSQLGNAAAAIT
                     ALECLRERLPVTMNDIRAGLLQVDIPGRFQVLPGRPAVILDVAHNPQAARALADSLKA
                     MGRTGRTFAVFAMLKDKDIAGVIAAVKSSVHHWFIAGLGGPRGASAAELGQALAVADV
                     KAVTACADVAAAYAQACDMATENDRIAVFGSFYTVAAVMPLRASRGPG"
     gene            687..1328
                     /locus_tag="AC158Q_04205"
     CDS             687..1328
                     /locus_tag="AC158Q_04205"
                     /inference="COORDINATES: protein motif:HMM:NF016895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR3339005.1"
                     /translation="MARTISDEELLLKRRARRRLIGAIVLVVAIVVVLPMVLDSEPGP
                     GGQTVSVQIPSSTSGVFASKVVPLAPAPESKAGPGRTLVAPAAEESKMITAAPPVSPP
                     PVPVKEAVQESPNDAVKQVPQPAAAKPRTKPVATPDGKYVVQVIALADAGKAKQMQER
                     ITDAGIKSYTEVVKTSKGDVTRVRAGPFATRGAAEKAREQLKILGMNGNIIAR"
     gene            1325..1816
                     /locus_tag="AC158Q_04210"
     CDS             1325..1816
                     /locus_tag="AC158Q_04210"
                     /inference="COORDINATES: protein motif:HMM:NF014704.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HIR3339006.1"
                     /translation="MTLFDLAVLVVIGLSILLSVIRGLVRELLALAAWVVAFLAANLL
                     AGRVATWLPDAVPGEEFRLLAGFLGVFLTVLIAMSVLAIMVSKLVKSAGLGLEDRLLG
                     SVFGFARGLLVVMVVVLLAGLTSLPRQAVWRNAVLSDPLEAFAGQVKAWLPTDLAQRI
                     TYD"
     gene            1832..3355
                     /gene="purF"
                     /locus_tag="AC158Q_04215"
     CDS             1832..3355
                     /gene="purF"
                     /locus_tag="AC158Q_04215"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIR3339007.1"
                     /translation="MCGIVGVVANNAVNQLLYDGLLVLQHRGQDAAGIVTADGANFRS
                     HKGPGMVRDVFRTRNMRSLAGFSGIGHTRYPTAGSAWSAAEAQPFYVNSPFGIVLGHN
                     GNLTNTEQLKQELFRQDLRHVNTNSDSEVLLNVLAHELQEGATNYKLDPATIFAAVSS
                     VYRRCRGAYAVVAMIAGYGLLAFRDAHGIRPLVFGRAETEQGYEYMVASESVALDSLG
                     FQVVRDVAPGEAIFIDQDGTFYNFQCVPNATLNPCIFEFVYLARPDSVIDGISVYETR
                     LRMGVSLAEKIRRQYRHLEIDVVIPIPDSSRPAAMELSKQLNLPYREGFIKNRYIGRT
                     FIMPGQGIRRKSVRQKLNSITMEFQGKNVLLVDDSIVRGNTSREIVQMARESGARRVF
                     FASAAPPVRYPNVYGIDMPTREELIACGRTEAEIAREIGCDELIYQDLEALREDVRSV
                     NPRVANFEDSCFSGIYVTGDVTREYLDGVEARRRDGAKMADMDEASTQLDLGLELVD"
     regulatory      3420..3496
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            3536..4768
                     /locus_tag="AC158Q_04220"
     CDS             3536..4768
                     /locus_tag="AC158Q_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase family protein"
                     /protein_id="HIR3339008.1"
                     /translation="MKNGFTTTILHSDLESPIEHFSVHKPMHMAVAFGYPDARDLAKV
                     FKGELAGYAYGRQGNPTTAALEVKVSKMEDGIATACFSTGMAAIAATFIALLRGGDHV
                     VSSSFLFGNTNSLFGTFDSLGCEISFVDATDADNVARAIRPNTRMVFVETIANPRTQV
                     ADLARIGELCASRGLLYVVDNTMTSPYLYRPKRNNAGLVINSLTKYIGGHGNALGGAV
                     TDTGLFDWGRFPNIYDSYKTAAPAMWGMLQIRKKGLRDVGGTLAAEVAHRLATGAETL
                     ALRMDKSAGNALQLARYLARHPKVTKVYYPGLEDHPQHRLASELFRNHGALMSIELAD
                     GIDCFDFLNRLKLVISSSHLGDNRTLAIPVAHTIYWEMGPGRRAEMGIAESLVRLSIG
                     IEDIDDLVGDFQQALAQA"
     gene            complement(4765..6417)
                     /locus_tag="AC158Q_04225"
     CDS             complement(4765..6417)
                     /locus_tag="AC158Q_04225"
                     /inference="COORDINATES: protein motif:HMM:NF023165.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HIR3339009.1"
                     /translation="MKALFKIWIVRLLALLIAGSISLSQTFAQDRVPFRQEELDQMLA
                     PIALYPDSLLSQVLMASTYPLEVVQASRWSRANPALKGQDAVRAVEGMDWDPSVKSLV
                     AFPQVLTAMDQKLEWTERLGEAFLAQQVDVMDTVQGLRRRAEAAGNLRSSEQMRVTHE
                     GEYIVIEPPGPQVVYVPYYSPVVVFGPWWWPAYPPVYWGPPPGYYAWPAYAPGFYWGS
                     GIVISAGFFFGHPNWHHRHVTVVHNHITHVTVNRQSTAVNRNVAAANARPVIWQHDPA
                     HRRGAPFRNPAARERFEKTRGPADARRDVNRTGSAQPARPADDNRTADIRQRRLEERR
                     AALQQNRREGKPEQQRDATVNRLTPGNNGDRAAAIRSAERERPAETRKAQPEARKAPA
                     ETRKAQPEARKSPAETRAPQPETRARPEPRRAQPERAAPQANDQRQERRDTPRPQTRG
                     IEQRPAVAAAPVARPAPRPAATTPSMRAEARQVPESRRRAEVRVNSQPASAMPRQSDV
                     ARERAPAPRPAASTRNHAPRMESHRPGGGNAGRQSAYPRTPS"
     gene            complement(6588..7310)
                     /locus_tag="AC158Q_04230"
     CDS             complement(6588..7310)
                     /locus_tag="AC158Q_04230"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF003743.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HIR3339010.1"
                     /translation="MPHTIFASDLHLTPDRPRANEIFFDFVSGPAAQADALYLLGDLF
                     EYWIGDDDLSDSFNASIADALRALSRNGVLLYFMQGNRDVLAGRDFVARCGATLIGDP
                     TLIDLHGIRTLLMHGDTLCSGDIEYQKFRLYAHDPENQRKFLAQPLAERREQMLGMRA
                     ESEKSKQGKSAGIMDVTPAAVEKMLRDSGYPRLIHGHTHRPGRHLHVVDGRECERWVL
                     TDWYGRAGYLRCDRTGCTAIAL"
     gene            complement(7408..7899)
                     /locus_tag="AC158Q_04235"
     CDS             complement(7408..7899)
                     /locus_tag="AC158Q_04235"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR3339011.1"
                     /translation="MVKLHTNHGTITIELDVKNAPQTVANFLQYVEDGHYSNTVFHRV
                     IDGFMIQGGGFEPGMKQKPTRAPVKNEADNKVKNAAYTLAMARTSEPHSASAQFFINV
                     ADNDFLNHTAPTPQGWGYCVFGRVTEGAGVVDRIKGVKTGRKGMHQDVPLEDVIIEKA
                     EVV"
     gene            complement(7946..8536)
                     /locus_tag="AC158Q_04240"
     CDS             complement(7946..8536)
                     /locus_tag="AC158Q_04240"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR3339012.1"
                     /translation="MLRTIAVLLTLLASLHAEAANPRVEMKTSLGTIVLELYPDKAPL
                     TVENFLQYAKDGHYNGTLFHRVIPGFMIQGGGFDAGFQQKPVRKPVKNEAANGLKNQV
                     GTIAMARTPDPHSATAQFFINVSDNASLDFRFPTAEGYGYTVFGKVVKGMDVVSRIVK
                     VPTGPGPAPHANVPLKPVVIESARLVTPSDKPAATK"
     gene            complement(8605..>8802)
                     /locus_tag="AC158Q_04245"
     CDS             complement(8605..>8802)
                     /locus_tag="AC158Q_04245"
                     /inference="COORDINATES: protein motif:HMM:NF048738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase Cds6 family protein"
                     /protein_id="HIR3339013.1"
                     /translation="VNAGKSWIRLKLDNVSVFLYPGREDLAVVTFDQDYASNNLSNQM
                     RKRQYWIREGGSWRILHEGAA"
BASE COUNT         1500 a         2667 c         2932 g         1703 t
ORIGIN      
        1 tcggcgcaga tttcggcgtc accctgcagg acaagcagtg gcagtattgg gggccgcgcg
       61 gcaagcgtaa tgcgctcccg cacccggcac tgcgcgggcc gtcccagctc ggcaacgcgg
      121 ctgcggcgat cactgcgctc gagtgtctcc gcgagcggtt gcccgtcacc atgaacgaca
      181 tccgcgccgg cctgctgcag gtggatattc ccggccgatt ccaggttctg ccggggcggc
      241 cggctgtgat actcgatgtg gcccataacc cgcaggcggc gcgcgcgctg gcggacagct
      301 tgaaagccat gggccgaacc gggcgcacgt tcgccgtatt cgcgatgctg aaggacaagg
      361 acattgccgg cgtgatcgct gccgtgaaat caagcgtcca tcattggttc atcgccggat
      421 tgggcggccc gcgaggggcc agtgctgccg aactcgggca ggcgttggcg gttgcggacg
      481 tgaaggcggt gacagcatgc gcggatgttg cagctgctta tgcgcaagcc tgtgatatgg
      541 cgacggaaaa tgatagaatc gcggtgtttg gatcgttcta cacggtggct gcggtgatgc
      601 cgttgcgggc atcccggggc ccgggataga tccggagtag tccgaggccc cgtattgctg
      661 atgggcggcg gaattgatgg cggcaaatgg cgcgaacgat cagcgacgaa gagctcctgc
      721 ttaagcgacg cgcaagacgg cgcctcatag gcgccatcgt gctggtggtt gccatcgtgg
      781 tggttctacc catggtgctc gactccgaac ccggcccggg cggacagacc gtttccgtcc
      841 agattccttc ctccacctcg ggggtttttg cgtccaaggt tgttccgctt gctccggcgc
      901 ccgagtccaa ggcgggaccc ggccggacgt tggtcgcgcc ggcagctgag gagtcaaaaa
      961 tgatcacagc cgcacctccc gtttcgccac cgccagttcc tgtcaaggag gcggtccagg
     1021 aatctcctaa cgatgcggtc aagcaagtgc cccaaccggc cgccgccaag ccccggacca
     1081 aaccagtcgc cacacccgac ggaaagtatg tggtgcaggt cattgcgctc gccgacgctg
     1141 gaaaggccaa gcagatgcag gagagaatca ccgacgccgg catcaagtcc tataccgagg
     1201 tggtcaagac ctcgaaaggc gatgtaacgc gggtgcgcgc gggcccgttc gccacgcgcg
     1261 gagccgcaga aaaggcgcgc gagcagctga aaatactggg catgaacggt aacatcatcg
     1321 cgagatgacc ctttttgatc ttgccgtact cgtggttatc gggttgtcga ttctgttgag
     1381 cgtgattcgg gggctggttc gcgagttgct ggcgctggcc gcctgggtgg tggcgtttct
     1441 tgccgcgaat ctgctggccg ggcgggttgc gacctggctg ccggacgcgg taccgggaga
     1501 ggagttccgg ctgctggccg gtttcctggg cgtgtttttg acggtgttga tagcgatgag
     1561 cgtgcttgcc atcatggtct cgaagctggt aaaaagcgcc ggcctcggtc tggaagaccg
     1621 gctgctgggg agcgtgttcg gattcgcccg gggcctgctg gtggtgatgg ttgtggtcct
     1681 gctggcgggg ctgacttcgc tgccccgaca ggcggtctgg cgcaatgcgg ttttgagcga
     1741 tccgctggaa gcgtttgcgg gccaggtcaa ggcctggctg ccgacggatc tggcgcaacg
     1801 catcacatat gactgaggtc gaggccgaac gatgtgcggg atagtcggtg tcgtcgccaa
     1861 taacgcggtc aaccagctgc tctatgacgg gctgctggtg ctgcagcacc gcggccagga
     1921 cgcggcgggt atcgtgaccg ccgacggcgc caatttccgc agccacaagg gccccggcat
     1981 ggtgcgtgac gtgttccgca cccgcaacat gcgctcgctg gccggcttct ccggaatcgg
     2041 acatacgcgc taccccacgg ccgggtccgc gtggtcggct gccgaggcgc aaccgtttta
     2101 cgtcaattct cccttcggca ttgtgctggg ccacaacggc aacctcacca acaccgaaca
     2161 gctgaagcag gaactgtttc ggcaggacct gcgccacgtc aacaccaatt ccgattccga
     2221 ggtgctgttg aatgtgctgg cacacgagct gcaggaaggc gccaccaact acaagctcga
     2281 tccagcgact attttcgcgg cggtgtccag cgtctaccgc cgttgccgcg gcgcctacgc
     2341 cgtggttgcg atgatcgcgg gttacgggct gctcgcgttt cgcgacgccc acggcatccg
     2401 gccgctcgtc ttcggccggg ccgagaccga gcagggctac gagtacatgg tggcttccga
     2461 gagcgtagcc ctcgattcgc tggggttcca ggtggtgcgc gacgtggcgc caggggaggc
     2521 gatttttatc gaccaggacg ggactttcta caatttccag tgcgtgccca atgccacgct
     2581 caacccgtgc attttcgagt tcgtctatct cgcacgcccg gactcggtga tcgacggcat
     2641 ctcggtctac gagacgcggc tgaggatggg cgtgagtctg gcggaaaaga tacgacgcca
     2701 gtaccgccac ctcgaaatag acgtggtgat tccgattccg gattcgagcc ggccggcggc
     2761 gatggagttg tcgaagcagc tgaacctccc gtaccgcgag ggcttcatca agaaccgcta
     2821 catcggccgc accttcatca tgccgggtca gggcatacgc cggaagtccg tgcgccagaa
     2881 gctcaactcg atcaccatgg agttccaggg caagaacgtg ctgctggtgg atgactcgat
     2941 cgtgcgcggc aacaccagcc gtgaaattgt gcagatggcg cgagagtccg gtgcacgcag
     3001 ggtgttcttc gcctccgctg cgccgccggt gcgctatccc aacgtctacg gcatcgacat
     3061 gccgacgcgc gaggagttga tcgcctgcgg caggaccgag gccgaaatcg cgcgcgagat
     3121 cggatgcgac gagctcatct accaggacct cgaggcgctg cgggaggacg tgcgcagcgt
     3181 taatcccagg gtcgccaatt tcgaggattc ctgcttcagc ggcatctacg ttaccggcga
     3241 cgttaccagg gagtatctcg acggcgtcga ggcccggcgg cgtgacggcg ccaagatggc
     3301 cgacatggat gaagccagca cccagctcga tctgggcctc gagctcgtcg actgaaccga
     3361 agcccgtggt gactcgcgag tggagccgga ttcttgacat ccgcctgatg gcggggtaac
     3421 tttccctccg cgccgatttg aagttcagct tgcgggcgcg ttaaaaatgc agctaaagcg
     3481 acgggagaac ccggtccgcg ctagctgacc gggtttttta tttgggagcc aaatcatgaa
     3541 gaacgggttt accacgacga ttctccattc cgacctggag tcgccgatcg agcacttttc
     3601 cgtgcacaaa ccgatgcaca tggcggtggc gttcggttat cccgacgccc gggatctggc
     3661 gaaggtgttc aagggcgaac tcgccggcta tgcctatggc cgccagggca atcccaccac
     3721 ggccgcgctg gaggtcaagg taagcaagat ggaggacgga atcgccaccg cgtgtttttc
     3781 caccggcatg gccgccattg ccgccacttt cattgcattg ttgcgcggcg gcgatcacgt
     3841 cgtgtcgagc tcgttcctgt ttggcaacac caacagcctg ttcggcactt tcgactcgct
     3901 gggatgcgag atcagttttg tcgatgcgac cgacgcggac aacgtggcgc gggcgatccg
     3961 gcccaacacc cggatggtat tcgtggaaac catagccaac ccgcgcaccc aggtcgccga
     4021 tctggcgcgc atcggagagc tgtgtgccag caggggattg ctctacgtgg tggacaacac
     4081 catgacctcg ccctatctgt accggccgaa gcggaacaat gccggcctgg taatcaattc
     4141 gctgaccaag tacatcggcg gccatggcaa tgcattgggc ggtgcggtga ccgacaccgg
     4201 cttgttcgac tgggggcgct tcccgaacat ttacgacagc tacaagacgg cggcccccgc
     4261 catgtggggc atgctgcaga ttcgcaagaa aggcttgcgc gacgtgggcg gcacgctggc
     4321 ggccgaggtt gcgcaccgcc tggcaaccgg cgccgaaacg cttgcgctgc gcatggacaa
     4381 gtccgccggc aatgcgctgc aactcgcccg ttacctggcc aggcatccga aggtgaccaa
     4441 ggtttattac cccggcctcg aggatcaccc tcagcaccgg ctcgccagcg agctgttccg
     4501 gaaccacggc gcgctcatga gcatcgagct ggcggatgga atcgactgct tcgacttcct
     4561 caaccggttg aaactggtga tttcatccag tcatctgggg gacaaccgga cactggcgat
     4621 cccggtggcg cacaccattt actgggaaat ggggccgggg cggcgcgccg agatgggaat
     4681 cgcggaatcg ctcgtccggc tgtcgatcgg catcgaggac atcgacgatc tcgttggcga
     4741 ttttcagcag gcgctggcgc aggcctagct cggcgtgcgc gggtacgcgc tctggcgacc
     4801 ggcgttgccg ccgccgggac gatggctctc catgcgcggg gcatggttcc gggtgcttgc
     4861 ggctgggcga ggagcggggg ccctttcgcg ggcgacgtcg ctttgccggg gcatggcgct
     4921 cgcgggctgg ctgttgacgc gaacttccgc acgccggcgg ctttcgggaa cctgccgggc
     4981 ctcggcgcgc attgagggtg tcgtggcggc cggacgggga gctgggcgag cgacaggcgc
     5041 tgccgcgacc gccggtcgct gctcgatgcc gcgcgtctgc ggacggggcg tatccctgcg
     5101 ctcctggcgc tggtcattgg cttgcggtgc ggcacgttcg ggttgagcac gacgcggttc
     5161 gggccgtgcg cgggtctcgg gctgtggggc acgcgtttcc gctggagact tgcgcgcttc
     5221 cggctgcgcc ttgcgcgttt ccgccggagc cttgcgtgct tccggctgcg ccttgcgcgt
     5281 ctcggccggc ctttcgcgct cagcactgcg aatcgctgcc gcgcggtcac cgttgttgcc
     5341 tggcgtgagc cggttgacgg ttgcatcccg ctgttgttct ggcttgcctt cgcgtctatt
     5401 ctgctgtagc gccgcccggc gctcttccag ccggcgctgg cggatgtccg ccgtccgatt
     5461 atcatcggca ggccgtgcgg gttgtgccga accagtccgg tttacatcgc gccgcgcatc
     5521 agcggggccc cgggttttct cgaaccgctc gcgtgccgct ggattgcgga agggcgcacc
     5581 ccggcgatgg gcaggatcat gctgccagat taccggccgc gcattggctg cagcgacgtt
     5641 gcggttaacg gccgtcgact ggcggttcac tgtcacatga gtaatgtggt tgtggacgac
     5701 ggtcacatgg cgatggtgcc agttggggtg accgaagaag aagcccgccg agatcacgat
     5761 gccactgccc cagtagaacc ccggagcgta tgcgggccat gcatagtagc ctggaggcgg
     5821 tccccaatag accggcggat aagccggcca ccaccacggc ccgaagacga cgaccgggct
     5881 gtagtacggc acgtagacga cctgcgggcc gggcggctcg atgacgatgt actcgccttc
     5941 gtgcgttacc cgcatttgct cgcttgacct gaggttgccg gctgcctcgg cacgccggcg
     6001 caggccctgc accgtgtcca tgacgtccac ctgctgcgcg aggaatgcct caccgagccg
     6061 ttccgtccac tcgagtttct ggtccatcgc ggtcagcacc tgggggaacg ccaccagcga
     6121 cttgacgctg ggatcccagt ccatgccctc tacggcccgc acggcgtcct gtcccttcag
     6181 tgcgggattg gcgcgcgacc agcgagaggc ctgcaccacc tccagcgggt aggtcgacgc
     6241 catcaggacc tgcgacaaca acgaatcggg atacaacgcg atcggcgcca gcatctgatc
     6301 gagctcctcc tgacggaagg gcacgcgatc ctgggcgaac gtctgggaaa gggaaatcga
     6361 ccccgcgatc agcagggcga gcaatcgaac gatccaaatc ttgaagagcg ccttcatcgt
     6421 cgtctcctcg taaatgcctg ttgcctattc gactgctttt tacgatgatc cgcacgttac
     6481 aaagtgtaag attttgtaaa gcggggcttt tccgcgcgca gccgcagaag aggaagagga
     6541 gaacggtgga tttgcgccgg cgcgcagcgg gggttgtcgc cggacggcta cagtgctatg
     6601 gccgtgcacc cagtccggtc gcagcgcagg tagccggcgc ggccgtacca gtcggtcagc
     6661 acccagcgct cgcattcgcg gccgtcaacg acgtgcaggt gccgccccgg gcgatgggta
     6721 tggccgtgga tgaggcgcgg gtagccggag tcgcgcagca ttttctccac ggccgcgggc
     6781 gtcacgtcca tgatcccggc gctctttccc tgcttgctct tttcgctctc cgcacgcatg
     6841 ccgagcatct gctccctgcg ctcagcaagt ggctgggcaa ggaatttccg ctggttttcc
     6901 gggtcatggg cgtagaggcg gaacttctgg tattcgatgt cgccgctgca cagcgtgtcc
     6961 ccgtgcatca gcagtgtccg aatgccgtgg aggtcgatca aggtggggtc cccgatcagc
     7021 gtggcgccac agcgcgccac gaaatcccgt ccggccagga catcgcggtt gccctgcatg
     7081 aaatagagca ggacgccatt cctgctcagc gcgcgcagcg catcggcaat cgaggcattg
     7141 aagctgtcgc tcaggtcgtc gtccccgatc cagtactcga acaggtcacc gagcaggtag
     7201 agcgcgtccg cctgtgctgc agggcccgac acgaagtcga agaagatctc gttcgcgcgt
     7261 ggccgatcgg gagtgaggtg gaggtcggag gcgaaaatgg tgtgtggcat aggattcggg
     7321 aatcaggatc caggattcag tgcggaacag tcgtactttc catcctcgcg atttctgaat
     7381 cccgaatccc gggtcctgaa tcctgtccta gaccacttcc gccttctcga tgatgacgtc
     7441 ctccagcgga acgtcctggt gcatgccctt acggccggtc ttgaccccct tgatcctgtc
     7501 caccaccccg gcgccttccg tcacgcgccc gaacacgcag tagccccagc cctgcggggt
     7561 gggggcggtg tgattgagga aatcattgtc ggcgacgttg atgaagaact gcgcgctggc
     7621 cgaatggggc tcggaggtgc gtgccatggc gagagtgtaa gccgcgtttt tcaccttgtt
     7681 gtcggcctcg tttttgaccg gcgcacgcgt gggtttctgc ttcattcccg gctcgaaacc
     7741 gccgccctgg atcatgaagc cgtcgatgac gcggtggaac acggtgttgc tgtagtggcc
     7801 atcctcgacg tactggagga agttggcgac cgtctgcggg gcgttcttga cgtcgagttc
     7861 aatcgtgatg gtgccgtgat tggtgtgcag tttgaccata atttcaagaa ccgggtgatg
     7921 aattgacgca gtgacgaggc gacggttatt tggttgctgc cggcttgtcc gaaggcgtga
     7981 cgagccgcgc gctttcgatg acgacgggct tgagcggcac gttggcatgc ggggcgggcc
     8041 ccggtccggt ggggaccttc acgatgcggc tcacgacgtc catgcctttt acgacttttc
     8101 cgaacacggt gtatccgtag ccctcggcgg tggggaaacg gaaatcgagc gaggcgttat
     8161 ccgacacgtt gatgaagaac tgcgcggtcg ccgaatgcgg gtcgggcgtg cgcgccatcg
     8221 cgatcgtgcc cacctggttt ttcagtccgt tggcggcctc gtttttcacc ggcttgcgca
     8281 ccggcttctg ctggaagccg gcatcgaacc cgcccccctg gatcatgaat cccgggatga
     8341 cccggtggaa aagggtgccg ttgtaatggc cgtccttcgc gtattgcagg aagttttcca
     8401 cggtaagggg cgccttgtcc gggtacagct cgaggacgat ggtgcccagg ctggttttca
     8461 tctcgacccg cggattggcc gcttcagcgt gaagggaagc gagaagggtg agcaatacgg
     8521 ctattgtcct gagcatgggt atatgaggag cggcaatttg aattgagtcg agggcgccgc
     8581 gtgagaccgg cgccggcacc ggtcttacgc ggcaccctcg tgcaggatgc gccacgagcc
     8641 gccttcccgg atccagtact gacgcttgcg catctgattg gagaggttgt tgctcgcgta
     8701 atcctggtcg aacgtcacga ccgcgaggtc ttcccggccc ggatagagga agacgctgac
     8761 gttgtcgagc ttcaacctga tccaggactt gccggcgttc ac
//
LOCUS       DBNGSG010000163         4778 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2261767, whole genome shotgun sequence.
ACCESSION   DBNGSG010000163 DBNGSG010000000
VERSION     DBNGSG010000163.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4778)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2261767"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="AC158Q_04250"
     CDS             <1..518
                     /locus_tag="AC158Q_04250"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HIR3339014.1"
                     /translation="GVVIGADGFGIAMDGGRWVKVPQIGRVVVGNDVEIGANTTVDRG
                     ALDDTVIEEGVKLDNQIQIAHNVHIGAHTAIAAFAGVAGSARIGRHCRIGGASGIAGH
                     LTIADHVEISTFTLVTKSIDKPGTYTGAYVFEPHNQWRRNAVQLRHLAELADRVRALE
                     KKQLQPKRSKP"
     gene            515..973
                     /gene="fabZ"
                     /locus_tag="AC158Q_04255"
     CDS             515..973
                     /gene="fabZ"
                     /locus_tag="AC158Q_04255"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HIR3339015.1"
                     /translation="MSGMDINQILEYLPHRYPFLLVDRVLSCEPGKTITALKNVSINE
                     PFFQGHFPNYPVMPGVLIIECMAQAAAILTFHSEKARPDKRSVYFFVGIDNARFKKPV
                     VPGDALRLEVAITRHVRGIWKFAAQAWVGESMAAESEIMCTVRSIREEGV"
     gene            1006..1779
                     /gene="lpxA"
                     /locus_tag="AC158Q_04260"
     CDS             1006..1779
                     /gene="lpxA"
                     /locus_tag="AC158Q_04260"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HIR3339016.1"
                     /translation="MKVHSTAIIDPGARIAPDVEIGPYTVIGPHVEIGPGCRIGPHVI
                     ITGHTRIGSRNRFFQFSSIGEMPQDKKYQGEATRLEIGDDNTVREFCTFNRGTAQDAG
                     ATRVGNGNWFMAYVHLAHDCQVGNSTIFANNAQLAGHVHIGDHVILGGYTGVHQFCRI
                     GAHSITGIGSVVLQDVPPYITASGDTAKPYGINVEGLKRRGFTAGAITGLRRAYKSLY
                     RSSLTLEQAKKALARQVKECPEVQVMLDFLSSSTRGIIR"
     gene            1789..2952
                     /gene="lpxB"
                     /locus_tag="AC158Q_04265"
     CDS             1789..2952
                     /gene="lpxB"
                     /locus_tag="AC158Q_04265"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007585388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HIR3339017.1"
                     /translation="MPEAPLTIGVVAGEASGDLLGAHLMRALREHLPQARFTGIGGPR
                     MQAVGLEALYPMEKLAVRGYVEVLRHYLEIAGIRRALAKHYLAEPPALFVGVDAPDFN
                     LGLELKLRSAGIPTAHFVSPSIWAWRGGRIHMIKRAVSRMLSIFPFEQPLYEQAGIPV
                     TYVGHPFAEMLAQVPDRAAAREQLRLPAAGKIIALLPGSRVSELEQMADLFIETARQI
                     VAGLPDAQFLVPLVNRPTRELFETALYRHDASGLNLTVLFGHAHEAMAAADVVLAASG
                     TATLEAALLGRPVVITYRMPRLSYWMMKGRGYLPYVGLPNILAGEFVVPEFLQDEANP
                     ENLSQAVLNLMFDTKVRIRLEQRFSTIAGELRQNSGERIADALLPLIRGGAVP"
     gene            2949..3527
                     /gene="rnhB"
                     /locus_tag="AC158Q_04270"
     CDS             2949..3527
                     /gene="rnhB"
                     /locus_tag="AC158Q_04270"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HIR3339018.1"
                     /translation="MNLLCGVDEAGRGPLAGPVFAAAVILDARHPIEGLADSKKLNAR
                     QRESLAREICAHATAWAVASASVEEIDSINILQASLLAMRRAVEQLPVMPARVLVDGL
                     HRPRLAVPVQAIVRGDATVAEIAAASILAKVARDGEMLALHLLHPLYGFDRHKGYPTS
                     AHLQALREHGVSSVHRRSFAPVRVLLGTPDQA"
     gene            3541..4335
                     /locus_tag="AC158Q_04275"
     CDS             3541..4335
                     /locus_tag="AC158Q_04275"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR3339019.1"
                     /translation="MKKIDSPANPRFKELRQLVESSRERRKAGRSVLDGTHLVTAYLE
                     RVGLPQQVAVSRKGLESPEIRTLLGRMKGVDTVMLGDALFKALSSVVTPTGILAVIET
                     PRSRPIAPRTESCVMLEDLQDPGNLGSILRSCAAAKIGHVLLSSGSVHAWSPRVLRAA
                     MGAHFALAIYEGVDLRAAARGFSGKRVATHQQSPGSLYEADLTGNLALLLGNEGSGLS
                     PGLIAAADTVVSIPMPGKAESLNVAAAAAVCLFERVRQLAAKKRNS"
     gene            complement(4414..>4778)
                     /locus_tag="AC158Q_04280"
     CDS             complement(4414..>4778)
                     /locus_tag="AC158Q_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HIR3339020.1"
                     /translation="VSRCGKTPTCLYLAMQFGISAANYPLIPEDFSRMQLPTPLRSLR
                     GKLYGLTIRPERLQQIRNERKPGSQYATITNCEFEVREAEALMRQEGIPFLDATSKSV
                     EELATTILHEAKLVRRIY"
BASE COUNT          867 a         1501 c         1573 g          837 t
ORIGIN      
        1 ccggcgtggt gatcggcgcg gacggattcg gcatcgcgat ggacggggga cgctgggtca
       61 aagtcccgca gatcgggcgt gtggtggtcg gcaatgacgt cgagatcggc gccaatacca
      121 cggtggaccg cggcgcgctc gacgacacgg tcatcgaaga gggggtcaag ctcgacaacc
      181 agatccagat cgcccataac gtccacatcg gcgcccatac cgcgatcgcc gcgttcgccg
      241 gagtggcagg cagcgcaaga atcggccgtc actgccggat cggcggcgca tcgggaatcg
      301 ccggccatct gaccatagcc gaccacgtcg agatctcgac tttcacgctc gtcaccaaat
      361 ccatagacaa gcccggcacc tatacgggcg cttacgtgtt cgaaccgcat aaccagtggc
      421 gcagaaatgc ggtgcagctg cgccatctgg ccgagctggc cgaccgcgta cgcgctctcg
      481 agaaaaaaca gttgcagccg aagaggagca agccgtgagc ggcatggata tcaaccagat
      541 actcgaatat cttcctcatc gatacccgtt tctgctggtc gaccgggtgc tgtcctgcga
      601 gccgggaaag acgattacgg cgctgaagaa tgtgtccatc aacgagccgt ttttccaggg
      661 acatttcccc aactacccgg tgatgcctgg ggtgctcatc atcgagtgca tggcccaggc
      721 ggccgcgatt ttgacgttcc acagcgagaa ggccaggccg gacaagcgtt cggtgtactt
      781 cttcgtcggg atcgacaacg cccgcttcaa gaaaccggtc gtccccgggg atgcgctgcg
      841 gctggaagtc gccattacgc gccatgtgcg tggtatctgg aaattcgccg cccaggcatg
      901 ggtaggcgaa tccatggcgg cagaatccga gatcatgtgc acggtccgct ccatcaggga
      961 ggagggggtg tgagcgggcc tgcgcccgtt cccctgcgag gccccgtgaa ggtccattcc
     1021 acagccatca tcgatcccgg ggcgcgcatc gcgccggacg tagagatcgg gccgtacacg
     1081 gtaatcggcc ctcacgtcga gatcgggcca gggtgccgga tcggaccgca tgtcattatc
     1141 accgggcaca cgcgcatcgg gtcccgcaac cgcttcttcc agttctcctc gataggagag
     1201 atgccccagg acaagaaata ccagggggaa gcaacgcgtc tggagatcgg cgacgacaac
     1261 acggtgcgcg aattttgcac gttcaaccgc ggcacggccc aggatgccgg cgccacgcgg
     1321 gtcggcaacg gcaactggtt catggcgtac gtgcaccttg cgcacgactg ccaggtcggc
     1381 aacagcacca tattcgccaa caatgcgcag ctcgccgggc acgtgcatat cggcgatcac
     1441 gtgattctcg gcggctatac cggcgtgcac cagttctgcc gcatcggcgc gcacagcatc
     1501 accgggatcg gctcggtggt gctgcaggac gtgccgcctt atatcaccgc ttccggcgat
     1561 accgccaagc cttatggcat caacgtcgaa ggcctgaagc ggcgcgggtt caccgccggc
     1621 gccatcaccg ggctcaggcg ggcctacaaa tcgctgtacc gctcgagcct gacgctggag
     1681 caggcgaaga aggcgctggc gcgccaggtc aaggaatgcc cggaagtcca ggtaatgctt
     1741 gatttcctgt cgagctcgac gcgcggcatt atccgttagc ctcgctgcat gcctgaagct
     1801 ccgcttacca tcggcgtggt cgcgggcgaa gcctcgggag acctcctggg ggcgcatctg
     1861 atgagggcgc tgcgcgaaca cctgccgcaa gcgcgtttca ccggtatcgg cgggccgcgc
     1921 atgcaggcgg tggggctgga agcgctctac ccgatggaaa agctggcggt aagaggatat
     1981 gtcgaggtgc tgcgccatta cctggaaatc gccgggatac ggcgcgccct ggcaaagcac
     2041 tatctggccg agccccccgc gctgttcgtg ggcgtggacg cgcccgattt caatctcggg
     2101 ctcgaactga aactgcgctc cgccggcata cccacggcgc attttgtcag cccctcgatc
     2161 tgggcgtggc gcggcgggcg catccacatg atcaagcgcg cggtgtccag gatgctttct
     2221 atttttccgt tcgagcagcc gctctacgag caggcgggca ttccggtcac ctacgtcggg
     2281 cacccatttg cggaaatgct ggcgcaggtc ccggatcgcg cggcggcgcg cgagcaactg
     2341 cgcctgccgg cggcgggcaa gatcatcgcg ctgctgcctg gcagccgggt gagcgaactc
     2401 gagcagatgg ccgatctttt catcgaaacg gcccggcaga tcgtggcggg cctgcctgac
     2461 gcgcagttcc tcgtgccgct ggtcaaccgg cccacccggg aactgttcga aaccgcgctc
     2521 tatcggcacg atgcgagcgg gctgaacctg acggtactgt tcggccatgc gcacgaagcg
     2581 atggcggcgg cggacgtggt cctggcggct tccggcaccg ccacgctgga ggcggcgttg
     2641 ctgggacgcc cggtggtgat cacctaccgc atgccgcgcc tctcatactg gatgatgaag
     2701 gggcgtggct acctgccgta cgtcgggctg cccaacatac tcgccgggga gttcgtcgtt
     2761 cccgaattct tgcaggacga ggcgaacccc gagaacctgt cgcaggcggt cctcaatctg
     2821 atgttcgata ccaaggtgag aattcgtctc gagcagcgtt tttctactat cgcgggggaa
     2881 ctgcgacaga acagcggcga gcgaattgca gatgcgctgc ttccactgat acgcggtggc
     2941 gcggtgccat gaacctcctg tgcggagtgg atgaagcggg gcgcggcccg cttgcagggc
     3001 cggtattcgc cgccgcggta atactcgacg cgcggcatcc catcgaaggg ctcgccgact
     3061 ccaagaagct gaacgcgcgc cagcgcgaat cgcttgcgcg ggagatatgc gcgcatgcca
     3121 ccgcctgggc ggtcgccagc gccagcgtgg aggaaatcga ttcgatcaat atcctgcagg
     3181 cgagcttgct ggccatgcgg cgcgcggtgg agcaactgcc ggtgatgccg gcgcgggtac
     3241 tggttgacgg actgcatcgg ccgcgcctgg ccgtgccggt gcaggccatc gtccggggcg
     3301 atgcaacggt ggcggaaatt gccgccgcct cgatactcgc gaaggtcgcc cgagacgggg
     3361 aaatgcttgc cttgcacctg ctgcaccccc tgtacggctt cgaccgccac aaaggctatc
     3421 ccacgagcgc gcacctgcag gcgctgcggg aacacggggt ttcgtccgtg caccggcgca
     3481 gctttgcccc ggtgcgcgtg ttgctgggaa cgccggacca ggcttgatcg cgcgcattcc
     3541 atgaagaaga tcgattcgcc cgccaacccg cgcttcaagg agttgcgcca gctcgtggaa
     3601 tcctcccggg aacgcaggaa ggccgggcgc tcggtgctcg atggaaccca cctcgtaacc
     3661 gcttatctgg agcgggtcgg gctgccgcag caggttgccg tcagccggaa ggggctcgag
     3721 agcccggaga ttcgcacgct gctgggcagg atgaaagggg tggataccgt catgctgggc
     3781 gacgcgctgt tcaaggcgct gtcatcggtg gtgacgccga ccggtattct ggcggtgatc
     3841 gaaacaccgc gctcgcggcc catcgcgccc agaacggaat cgtgcgtgat gctggaagac
     3901 ctgcaggacc cgggcaacct cggctccatc ctgcgctcgt gcgcggccgc gaaaatcggt
     3961 cacgtgctgc tgtcgagcgg ctccgtgcac gcctggtcgc cgcgggtgct gcgggcggcg
     4021 atgggggccc attttgcgct ggccatatac gagggcgttg acctgcgggc ggccgcgcgc
     4081 ggcttctccg gcaaacgggt agccacgcat cagcaatcgc ccggctcgct gtacgaggcg
     4141 gacctgacag gcaaccttgc gctcctgctc ggaaacgagg gttcagggtt gagtcccggc
     4201 ctgatcgcgg cggcggacac cgtcgtctcg ataccgatgc cgggcaaggc cgaatcactc
     4261 aatgtcgcgg ccgcagctgc ggtatgcctg ttcgagcggg tgcggcagct tgcggcaaaa
     4321 aagcgcaatt cgtgaatcgt ttcattcgaa caagtcaccc acaccgcgca gattcatccc
     4381 cggatcacga ctcatcactt acgaatcacg gtttcaataa atccggcgca cgagcttggc
     4441 ctcgtgcagg atcgttgtgg ccagttcctc gactgatttg ctggtggcat cgagaaaggg
     4501 gataccttcc tgccgcatca gcgcttcagc ctcgcgcact tcgaactcgc aattggtgat
     4561 cgtcgcgtac tggctcccgg gtttgcgttc gttgcgtatc tgctgcaggc gctcgggccg
     4621 gatggtcaat ccgtacagct tgccccgcag cgaacgcaat ggggtgggca actgcatacg
     4681 gctgaaatcc tccgggatga gcgggtagtt ggccgcgctg atgccgaatt gcatcgccag
     4741 gtacaaacag gtcggcgtct tgccgcagcg cgaaaccc
//
LOCUS       DBNGSG010000164         5444 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2091834, whole genome shotgun sequence.
ACCESSION   DBNGSG010000164 DBNGSG010000000
VERSION     DBNGSG010000164.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5444)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5444)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5444
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2091834"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..1690
                     /locus_tag="AC158Q_04285"
     CDS             116..1690
                     /locus_tag="AC158Q_04285"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HIR3339021.1"
                     /translation="MKIQPVVLCGGSGTRLWPLSREQHPKQLLALNGDLSLLQETLRR
                     MDGLAIKPAPAAAPVVLCQEEYRFLVNDQLRAIGAQARSIILEPAGKGTAPALTLAAL
                     HVVKDDDPVLLVMPSDHVIERREAFHDAIRRGAALAAEGCIVTFGIPPAAPETGYGYI
                     RVGNELYPGRTPPAYDVDSFVEKPDGETAQRYAASGSYLWNSGIFMVRGSIWLEVLGR
                     LRPEMLAACELAHGQGARDGDFYHIDARAFEECPQDSIDYAVMEQMGPRRAGSKGPRA
                     AAVRLEAGWSDVGAWDALWQIGGKDGDGNVVHGDVCAVDTRKALLIARHRLLACVGLE
                     DVVVIETADAVMVARRDQAQAVKQVVSRLKSQGRRECVTHRKVHRPWGSYESVDGGER
                     FQVKRIIVNPGAALSLQIHYHRAEHWIVVRGTARVTRGDQAYLLTENQSTYIPLGVKH
                     RLENPGRVPLEIIEVQSGAYLGEDDIVRFEDTYGRAEKSQAAGVTPAPSLSAVPKPAA
                     GDAAGPARSTGAKGGA"
     gene            1687..2688
                     /gene="galE"
                     /locus_tag="AC158Q_04290"
     CDS             1687..2688
                     /gene="galE"
                     /locus_tag="AC158Q_04290"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HIR3339022.1"
                     /translation="MKILVVGGAGYVGSHMTKMLLDEGHEAVVVDNLSSGYRDAVPAR
                     VLVKADIANRVLIDSIFTTRRFDGVMHFASSIQVGESVQHPSKYYWNNVAGTLNLLDA
                     MVKHHVKRIVFSSTAAIFGEPGYVPIDEEHRKAPLNPYGRSKWMVEQLLADYERAYGL
                     RHSCLRYFNAAGADPSGRLGERHDPETHLIPLVLRAAAGRLPSIGIFGTDYDTPDGTC
                     IRDYVHVNDLCRAHLLAMQHLMDGGRSRPFNLGYGSGYSVREVIDIASKVTGRPIPVI
                     ELARREGDPERLVSDPSRACRELGWQPQDSDLETIIAGAWVWEQARQALPHAHAAFR"
     gene            complement(2715..3980)
                     /gene="epsL"
                     /locus_tag="AC158Q_04295"
     CDS             complement(2715..3980)
                     /gene="epsL"
                     /locus_tag="AC158Q_04295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtB/PEP-CTERM-associated polysaccharide
                     biosynthesis outer membrane protein EpsL"
                     /protein_id="HIR3339023.1"
                     /translation="MRGKTIAIIALACSPATSAAFQTIEELPWPESGRFPAYSREPDD
                     GRNVRFNVFGGAMRDNNVFRIPDGTPPRPIIGSSDRADNIYRYGAELKADIPASRQRI
                     LFEAQLEHRDYEQFGFLDHDAYRLGAAWKWAAGPQWSGDIGYGKRRFLASLAELQAPI
                     KDLITEDRAYASAGYRFTPRWRVRGGVESYDWKHSDPTRIALDNRTSSGTVGLDYVTP
                     QENSIGGQFKYSYGDYPNRQVVPGGTVDNNFDEYESSAVAHWAVTGKSTLDARLGYTS
                     RVHDQLPQRDFDGVTGRLNYDWFVAAKTILNFAVWREIRSIEDVDASYVLSEGWGIGP
                     SWAPTSKLVFQAKYVREDRDYRGDPGFVLTGGPQREDTFRGLRLAAGYAPRRNIELAV
                     GLETGDRSSNVAGRDYDYTAVMANARLKF"
     gene            4749..>5444
                     /locus_tag="AC158Q_04300"
     CDS             4749..>5444
                     /locus_tag="AC158Q_04300"
                     /inference="COORDINATES: protein motif:HMM:NF025104.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-fold NAD(P)-binding domain-containing
                     protein"
                     /protein_id="HIR3339024.1"
                     /translation="MSTLTAEFQPAATELPLVALVRVMLAPVVCTLCLALSLAIHRED
                     FSVHYGMLAVVAFLVSMQVFGELPLTNGRSRMSFLLPGRTIMVKWVTVIGILLFIAFA
                     AKVTGLFSRRVVLAWLAITPFALQTAQEIVRLLLHRLVSSSAAGRVKVVVGVNDTACE
                     LTRRIGEDPCHGTFMGFFDDRGGGRIGGVGQEQVLGGLADVVDSVKRNRINVVYITLP
                     LTRDPRIQRLLDGL"
BASE COUNT          902 a         1766 c         1871 g          905 t
ORIGIN      
        1 caaaaggcaa gaggaaaaag gaaaaaggaa gaaaacacaa ggcgatggtg aacagggcta
       61 ccgactcacg actcacgaat cgggaatcgc gaacattcaa cgagcggagg gcatgatgaa
      121 gatccaaccg gtagtattgt gcggaggctc cggaacgcgc ctgtggccgc tgtcgcgcga
      181 acagcatccg aagcagttgc ttgcgctgaa tggggacttg agcctgctgc aggagacgct
      241 ccgccgcatg gacgggctgg cgatcaagcc ggcgccggcg gcggcaccgg tggtcctgtg
      301 ccaggaggaa taccgctttc tcgtcaacga ccagttgcgc gcgatcgggg cgcaggcgcg
      361 ctccatcatc ctcgagccgg cgggcaaggg cacggccccg gcgctcacgc tcgcggcgct
      421 gcacgtggtc aaggatgatg acccggtgct cctcgtcatg ccttccgacc atgtcatcga
      481 gcggcgggaa gcgttccatg acgcgatccg ccgcggcgcg gccctggccg ccgaaggctg
      541 tatcgtcacc ttcggcatcc ccccggccgc gcccgaaacc ggctatggct acatacgcgt
      601 cggcaacgag ctctatcccg gccgcacgcc gccggcgtac gacgtcgatt cgttcgtcga
      661 gaagccggac ggcgagacgg cacagcgcta cgccgcctcc ggatcgtatc tgtggaacag
      721 cgggatattc atggtgcgcg gctcgatctg gctcgaggtc ctcggccgcc tgcgcccgga
      781 aatgctggcg gcctgcgagc ttgcgcacgg ccagggcgcg cgcgacggcg atttctacca
      841 catcgacgcg cgggcattcg aggaatgccc gcaggattcc atcgactacg cggtgatgga
      901 gcaaatgggg ccgcggcggg cgggaagcaa gggcccgcgc gctgcggcgg tcaggctgga
      961 agcgggctgg tccgacgtgg gcgcgtggga cgcgctgtgg caaatcggcg gcaaggacgg
     1021 cgatggcaac gtggtgcacg gcgacgtttg cgcggtggat acccgcaagg cgctgctgat
     1081 cgcgcgccac cgcctgctgg cgtgtgtcgg cctggaagac gtggtggtga tcgaaacggc
     1141 cgatgcggtc atggtggcgc ggcgggacca ggcgcaggcc gtgaagcagg tggtctcccg
     1201 cctgaagagc cagggccgcc gggaatgcgt gacgcaccgc aaggtgcacc ggccgtgggg
     1261 aagctatgaa agcgtcgacg gcggcgagcg tttccaggtg aagcgcatca tcgtcaaccc
     1321 cggcgccgcg ctatcgctgc agattcacta ccaccgcgcc gagcactgga tcgtggtgag
     1381 ggggacggcc cgggtgacgc gcggcgacca ggcctacctg ctgacggaga accagtccac
     1441 ctacattccg ctcggcgtga agcaccgcct ggagaacccg ggacgcgtgc cgctcgaaat
     1501 catcgaggtg caatcgggcg cgtatctcgg cgaggacgac atcgtccgct tcgaggatac
     1561 ctacgggcgc gctgaaaaga gccaggctgc gggcgtcacg ccggccccgt cgctgagcgc
     1621 cgtgccgaag ccggcggccg gagacgcggc ggggccggca cgcagcacgg gcgcgaaagg
     1681 cggcgcatga agatactcgt tgtcggcggc gccggctacg tcggctccca tatgacgaag
     1741 atgctgctcg acgaggggca cgaagcggtg gtcgtggaca acctgtcgag cggctaccgc
     1801 gacgcggtcc cggcgcgggt cctggtgaag gcggacattg ccaaccgcgt cctgatcgac
     1861 agcatcttca ccacgcgccg cttcgacggc gtaatgcatt tcgcatccag catccaggtc
     1921 ggggagtcgg tgcagcatcc ctcgaaatac tactggaaca acgtcgccgg caccctcaac
     1981 ctgctcgacg cgatggtgaa gcaccacgtg aagcggatcg ttttctcctc gacggcggcg
     2041 atcttcggcg aaccgggcta tgttccgatc gacgaggagc accgcaaggc gcccctcaac
     2101 ccctacggcc gctccaagtg gatggtcgag cagctgctcg ccgattacga gcgcgcctac
     2161 ggcctgcggc acagctgcct gcgctatttc aacgccgccg gcgccgatcc gtccggcagg
     2221 ctcggcgagc ggcacgaccc cgagacgcat cttattccgc tggtgttgcg cgcggcggcc
     2281 gggcggctgc cgtcgatcgg catattcggc acggactacg acacgccgga cgggacctgc
     2341 atccgcgatt acgttcacgt gaacgatctg tgccgcgccc acctgctggc gatgcagcac
     2401 ctgatggacg gcgggcgcag caggccgttc aacctcggct acggcagcgg ttactccgtg
     2461 cgggaagtca tcgacatcgc cagcaaggtg accgggaggc cgatcccggt catcgagctc
     2521 gcgcggcgcg aaggcgaccc ggaacgcctc gtctcggatc cgtcgcgggc ctgccgggaa
     2581 ctgggatggc agccccagga ctcggacctg gagacgatca tcgccggcgc atgggtctgg
     2641 gagcaggcgc ggcaggccct tccgcacgct cacgccgcgt tccgctagcg cgcgcgggca
     2701 gggcgccgcc cgtctcagaa cttcaaccgc gcgttggcca tcaccgccgt gtagtcgtaa
     2761 tcgcgcccgg ccacgttgga gctgcggtcg ccggtctcca gcccgactgc cagttcgatg
     2821 ttgcgccggg gcgcgtaacc ggctgccagg cgcagaccgc ggaaggtgtc ctcgcgttgg
     2881 ggccctcccg tcagcacgaa gccgggatca ccccggtagt cgcgatcctc gcgcacgtac
     2941 ttcgcctgaa agacgagctt ggaggtgggc gcccaggagg ggccgatacc ccagccttcg
     3001 gagagcacgt agctcgcgtc gacgtcctcg atcgagcgga tttcccgcca caccgcgaag
     3061 ttgaggatgg tcttggccgc gacgaaccag tcgtaattca accgcccggt gacgccgtcg
     3121 aagtcccgct gggggagctg atcgtgtacg cggctggtgt agcccaggcg ggcgtcgagc
     3181 gtggacttgc cggtcaccgc ccagtgcgcc acggcgctgc tttcgtattc gtcgaaatta
     3241 ttatcgaccg ttccgcccgg gacgacctgc cggttcgggt aatcgccgta actgtacttg
     3301 aactggccgc cgatggagtt ctcctgcggc gtcacgtagt cgagcccgac cgtgccggag
     3361 ctggtgcggt tgtcgagcgc gatgcgggtc gggtcgctgt gcttccagtc gtaggactcc
     3421 accccgccgc gcacccgcca gcgcggcgtg aaccggtagc cggcgctcgc gtacgcgcgg
     3481 tcttccgtga tcaggtcctt gatcggcgcc tgcagttcgg cgaggctggc caggaaacgc
     3541 cgcttgccgt agccgatgtc gccgctccat tgcggcccgg ccgcccattt ccaggccgcg
     3601 cccagccgat aggcgtcatg gtcgagaaat ccgaattgct cgtaatcccg gtgctcgagc
     3661 tgggcttcga acaggatgcg ctgccggctc gccggaatat cggccttgag ctccgcgccg
     3721 tagcgataga tgttgtcggc ccgatcggac gagccaatga tcggccgcgg gggcgtgccg
     3781 tccgggatcc tgaacacgtt gttgtcgcgc atggcgcccc cgaacacgtt gaagcgcacg
     3841 ttgcgcccgt cgtccggctc tcgggaatac gcgggaaacc ggccgctttc cggccacggg
     3901 agttcctcga tggtctggaa ggcggcgctg gttgccggac tgcaggccag cgcaatgatt
     3961 gcgatcgtct tgccgcgcat tcttgttctc cttgtgtgaa aaatccccgg gacatctgcg
     4021 aaatccgtgc cagggcggcc ggcgcggagc ggggccggcg gcgcgtgaac tatatcaaca
     4081 aattgcgtag cgcttatgcc acccgcatgc acgggcagcg ggtttccggt cgaattgcgg
     4141 gtcgtcggtt gctcggaata cacgtgtttt caacaggttg cgagccgagg cagtcagtcg
     4201 tccgattgcg acaggaaaat cagtgaaaaa tcatgcccct tagaggcatt ctgcgattca
     4261 cgttgccgca aggcgacagc agccgggcac gcgccccgtg gcccgacttc cggatcgcat
     4321 cccgcgtcgt tccgcgtccg gtggtgtata ccgggtgcgc cgccgggcaa tttgccgtgc
     4381 tcgatcccgg tcgcgtccgc ccgatcggcc gcagccctcg catgccaccg caatccccgg
     4441 tttccgcgcg tgccggcggc gcgaaaagcg gcgcgaaatt tcgccgcggt catgcggaaa
     4501 atgcgcgcaa tcgcgcgtgt cgttccatgc agacattctg cacccgaaaa agcggtttcg
     4561 ccgggtgccg caacggcgcg tgcgcgccgc aactcgttga tctgagtagg gctacagcgg
     4621 tgctggcacg agtgttgcga ctccgggccc agtggcgcgg gcgcggcctg tgtcatgtcg
     4681 tgtccaggcg cgcgacgttc agccgcggta accggagcaa aacaagcatt gcggccagcg
     4741 taaccaccat gagcacgtta accgcagaat tccagccagc cgcaacggaa ctcccgttgg
     4801 tcgccctggt gcgcgtcatg ctcgcgccgg tggtgtgcac gctgtgcctg gcactgagcc
     4861 tggccattca ccgggaggac ttttcggtcc attacgggat gctcgccgtc gttgcctttc
     4921 tcgtttcgat gcaggttttc ggcgagctgc cgctgaccaa cggccgttcg cggatgtcct
     4981 tcctcctgcc gggacggacc atcatggtca agtgggtgac ggtgatcggg atattgctgt
     5041 tcatcgcgtt cgcggccaag gtcaccggcc ttttttcgcg cagagtcgtt ctggcctggc
     5101 tcgcgatcac gccgttcgcg ctgcagaccg cgcaggaaat cgtgcggctg ctgctgcacc
     5161 ggctggtgtc ctcgagcgcc gcggggcgcg tcaaggtcgt ggtgggcgtc aacgatacgg
     5221 cgtgcgagct gacgcgccgc atcggcgaag acccgtgcca cggcacgttc atgggattct
     5281 tcgacgaccg cggcggcggc cggattggcg gggtcgggca ggagcaggtg ctcggcggcc
     5341 tggcggacgt cgtcgacagc gtcaagcgca accggatcaa cgtcgtctac atcacgctgc
     5401 cgctcacgcg cgatccgcgg atccagcgcc tgctggacgg gctg
//
LOCUS       DBNGSG010000165         5303 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_904450, whole genome shotgun sequence.
ACCESSION   DBNGSG010000165 DBNGSG010000000
VERSION     DBNGSG010000165.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5303)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5303)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5303
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_904450"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="AC158Q_04305"
     CDS             complement(<1..308)
                     /locus_tag="AC158Q_04305"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HIR3339025.1"
                     /translation="MKYSFEERKVVCKGDYWIAPDAIVIGSVVMEPNASIWFGCIVRA
                     DNDTVTIGENSQLQDGCVLHVDPGFPITIGRNVSVGHMAMLHGCTIGDGSLIGIKAVL
                     L"
     gene            complement(436..2508)
                     /locus_tag="AC158Q_04310"
     CDS             complement(436..2508)
                     /locus_tag="AC158Q_04310"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HIR3339026.1"
                     /translation="MEYGLLFALICSVIALVYGAVSIQWILAQPAGNARMQEIAAAIQ
                     QGAGAYLNRQYKTIAVVGAVLFVLIGVVLHSWTTAVGFAIGAILSGLAGYVGMNVSVR
                     SNVRTAEAARKGLNEALTIAFRGGAITGMLVVGLGLLGVTGFFWVLHESAPHGAAIKD
                     IAHPLIGLAFGGSLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIADN
                     VGDNVGDCAGMAADLFETYAVTIIATMLLGALLLSKPSVAPLAVAYPLALGGVSIIGS
                     IIGCYFVKARSGGKIMNALYRGLIMSGAISAVGFALVTWWMMGGITEQYPALTPIRLF
                     GAALVGLILTALMVVITEYYTATEYGPVRHVAAASTTGHATNIIAGLGVSMRSTAWPV
                     IAVCASIYAAYQLAGLYGLAIAATGMLSMTGIIVALDAYGPITDNAGGIAEMANLPKE
                     VRAITDPLDAVGNTTKAVTKGYAIGSAGLAALVLFADYTHALEAAGKAVEFSLSDPAV
                     VIGLFIGGLVPYLFGAMAMEAVGRAAGSVVVEVRRQFKEIKGIMEGTAKPDYSRAVDM
                     LTRSAIKEMMIPSLLPVLVPIVVALGMNWLMGPGAGVKALGGVLMGTIVTGLFVAISM
                     TTGGGAWNNAKKYIEDGNHGGKGSEAHKAAVTGDTVGDPYKDTAGPAVNPLIKIINIV
                     ALLIVPLL"
     gene            complement(2609..3868)
                     /locus_tag="AC158Q_04315"
     CDS             complement(2609..3868)
                     /locus_tag="AC158Q_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR3339027.1"
                     /translation="MTQKNAFYAQSGGVTATINTTACGVIETARKHRNRIGRVYAGRD
                     GIIGALTEDLIDTSKEPAAAIRALRHTPGGAFGSCRYKLKGLEESRAQYERLIEVFKA
                     HDIGYFFYNGGNDSADTSYKVSQIARTLGYPLACIGIPKTVDNDLAITDTCPGFGSVA
                     KYVATSIREAGYDVASMARTSTRVFILEVMGRHAGWITAACGLAQDKEGEAPHILLFP
                     EIPFDQEKFLAKVDETVRRHGYCAIGVSEGVRWPDGKFLSESGLRDAFGHAQLGGVAP
                     LLAQLVKDKLKQKYHWAVADYLQRSARHIASKTDVLQSYAVGKVAVELAVKGRSGVMP
                     VIVRKSSKPYRWAIGVAELKDVANAEKMMPRDFITADGFHITAKCRQYLEPLVQGEDY
                     PPYRNGLPAYVKLKNAAVPKKLAAFKI"
     gene            complement(4003..4668)
                     /gene="adk"
                     /locus_tag="AC158Q_04320"
     CDS             complement(4003..4668)
                     /gene="adk"
                     /locus_tag="AC158Q_04320"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - AMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HIR3339028.1"
                     /translation="MRIILLGMPGAGKGTQAQFLMDKFRIPQISTGDMLRAAIKAGTP
                     LGKEAGGYMDKGELVPDRVVIELVKQRVKETDCAQGFIVDGFPRTIAQAEALRHSGID
                     IDYVVEIAVGDDEILRRMSGRRVHPASGRTYHVTFNPPRVPGKDDVTGEDLVQRADDN
                     EATVKRRIANYHSQTQPLIAFYSKWAANGDKRAPRVIKLPGTGKVEEIRDRMFAALAQ
                     SKK"
     gene            complement(4740..>5303)
                     /gene="kdsB"
                     /locus_tag="AC158Q_04325"
     CDS             complement(4740..>5303)
                     /gene="kdsB"
                     /locus_tag="AC158Q_04325"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HIR3339029.1"
                     /translation="AVMTRARHATGTDRLAEVARRRRYPPDRIIVNVQGDEPLISPAL
                     IRAVAADLARSRNARIATACHPLRSAREMRNPNVVKVALDGAGYALYFSRAPIPWARN
                     AFARGIRALPAGLPVYRHIGIYAYRAAFLRQYARLEPTALERFEALEQLRALANGIRI
                     HCAVTRSVPHPGVDTPADLRRLLRKLK"
BASE COUNT          881 a         1725 c         1776 g          921 t
ORIGIN      
        1 aggagcacgg ccttgatgcc gatcaggctg ccgtcgccga tggtgcagcc atgcagcatc
       61 gccatgtggc cgacgcttac attgcggccg atcgtgatcg ggaaccccgg gtccacgtgc
      121 agcacgcagc cgtcctgcag ctgggaattc tcgccgatgg tgacggtatc gttgtcggcg
      181 cgcacgatgc agccgaacca gatgctcgcg ttcggctcca tgacgaccga gccgatcacg
      241 atcgcgtccg gcgcgatcca gtaatcgccc ttgcagacga ccttgcgctc ctcgaagctg
      301 tatttcatcg gtgatcagga ccgcggtgag tggcagataa gcaattgaac cgccagccac
      361 gccaagagaa tcaatcaaac atcaagaatg ttcttgatcc tcttggcggc cttggcggtt
      421 catattggcg gttgactaca acagcggcac gatgagcagc gccacgatgt tgatgatctt
      481 gatgagggga ttcacggccg ggcccgccgt gtccttgtag gggtcgccca cggtgtcgcc
      541 ggtcacggcc gccttgtggg cttccgagcc cttgccgccg tggttgccgt cctcgatgta
      601 cttcttggcg ttgttccagg cgccgccgcc ggtcgtcatc gagatcgcga cgaacaggcc
      661 ggtgacgatc gtgcccatca gcacgccgcc cagcgccttc acgcccgcgc ccgggcccat
      721 cagccagttc atgcccagcg ccaccacgat cggcaccagc accggcagca gcgacgggat
      781 catcatctcc ttgatcgccg agcgcgtgag catgtccacg gcgcgcgaat agtcgggctt
      841 ggccgtgcct tccatgatgc ccttgatctc cttgaactga cggcgcacct cgaccaccac
      901 cgaacccgcc gcgcggccca cggcttccat cgccatggcg ccgaacaggt agggcaccag
      961 gccgccgatg aagaggccga tcaccaccgc gggatcggag aggctgaact cgaccgcctt
     1021 gcccgcggct tcgagggcgt gggtgtagtc ggcgaacagc accagggcgg cgaggcccgc
     1081 cgagccgatc gcatagccct tggtcaccgc cttcgtggtg ttgcccacgg cgtccagggg
     1141 atcggtgatg gcgcgcactt ccttgggcag gttcgccatc tcggcgatgc ccccggcgtt
     1201 gtcggtgatc ggcccgtagg catcgagcgc gacgatgatg ccggtcatgg agagcatgcc
     1261 ggtcgccgcg atggcgagcc cgtagagccc ggcgagctgg tacgcggcgt aaatgctcgc
     1321 gcataccgcg atcacgggcc acgcggtcga gcgcatcgaa acgccgaggc cggcgatgat
     1381 gttggtcgca tggcccgtcg tggaagccgc ggcaacgtga cgcacggggc cgtactcggt
     1441 cgcggtgtag tactcggtga tcaccaccat gagggcggtg aggatcaggc cgaccagggc
     1501 ggcaccgaag agcctgatcg gcgtcagcgc cgggtactgc tcggtgatcc cgcccatcat
     1561 ccaccaggtg acgagggcga aaccgacggc ggagatggcg cccgacatga tcaaaccccg
     1621 gtagagcgcg ttcatgatct tgccgccgga ccgcgccttc acgaaataac agccgatgat
     1681 ggagccgatg atcgagacgc cgcccagcgc gagcgggtag gccaccgcca gcggcgcgac
     1741 cgacggcttg gacagcagca gcgcgcccag cagcatggtg gcgatgatgg tcacggcgta
     1801 ggtttcgaag aggtccgccg ccatgccggc gcagtcgccg acgttgtccc cgacgttgtc
     1861 ggcgatcacc gcggggttgc gcggatcgtc ctcgggaatg ccggcctcga ccttgccgac
     1921 gaggtccgcc ccgacgtccg cgcccttggt gaagatgccg ccgccgagac gcgcgaagat
     1981 cgagatcagc gagccgccga acgcgaggcc gatcagcggg tgggcgatgt ccttgatcgc
     2041 cgcgccgtgg ggagccgact catgcagcac ccagaagaac ccggtcacgc cgagcaggcc
     2101 caggcccacc accagcatgc cggtgatggc gccgccgcgg aaggcgatgg tgagcgcctc
     2161 gttcaggccc ttgcgcgccg cttccgccgt gcgcacgttg gagcgtaccg agacgttcat
     2221 gccgacgtag ccggcgaggc ccgagagaat tgcgccgatc gcgaagccga ccgcggtcgt
     2281 ccaggaatgc aatacgacgc cgatcagcac gaacagcacg gcgccgacga cggcgatcgt
     2341 cttgtactgg cggttcaggt acgcgcccgc gccctgctgg atggcggcgg cgatttcctg
     2401 catgcgcgcg ttgcccgcgg gctgggcgag gatccactgg atcgatacgg cgccgtaaac
     2461 gagcgcgatg accgagcaga tcagtgcaaa caacagtccg tattccatga aaactccctt
     2521 ggtagctatt ttgcttattc gtgatttgtg attcgcgcgg gcggcctcag tgcgatagcc
     2581 ggccccgtcg attcacgatt cacgccgttc agatcttgaa tgcggcgagc ttcttcggca
     2641 ccgcggcatt cttcagcttc acgtaggccg gcagcccgtt gcggtacgga ggatagtcct
     2701 cgccctgcac cagcggctcg aggtactgcc ggcacttcgc cgtgatgtga aagccgtccg
     2761 cggtgatgaa gtcgcgcggc atcatcttct ccgcattggc gacgtccttc agttccgcca
     2821 cgccgatcgc ccagcggtac ggcttgctcg atttcctgac gatgaccggc atcactccgc
     2881 ttcggccctt caccgccagt tccaccgcga ccttgcccac cgcgtaggat tgcaggacgt
     2941 cggttttcga cgcgatatgg cgcgccgagc gctgcaggta gtcggccacc gcccagtggt
     3001 atttctgctt aagcttgtcc ttgaccagtt gcgcgaggag cggcgcgacg ccgcccagct
     3061 gggcatgccc gaatgcatcg cgcaggcccg actccgacag gaacttgccg tcgggccagc
     3121 gcacgccctc ggagacgccg atggcgcaat agccgtgccg gcgcacggtc tcgtcgacct
     3181 tcgcgaggaa tttctcctgg tcgaagggga tttccgggaa cagcaggatg tggggcgctt
     3241 cgccttcctt gtcctgggcg agcccgcacg ccgcggtgat ccagccggca tgccgcccca
     3301 tcacttccag gatgaagacg cgggtcgagg tgcgcgccat ggacgcgacg tcgtagccgg
     3361 cctcgcggat gctggtggcg acgtacttgg cgaccgagcc gaagccgggg caggtatcgg
     3421 tgatcgcgag gtcgttgtcc acggtcttgg ggatgccgat gcaggcgagg ggatagccca
     3481 gcgtccgggc gatctgcgag accttgtacg aagtgtcggc ggaatcgttg ccgccgttgt
     3541 agaagaaata gccgatgtcg tgcgccttga acacttcgat cagccgctcg tactgggcgc
     3601 gggactcctc caggcctttg agcttgtagc ggcaggaacc gaacgccccg cccggggtat
     3661 ggcgcaacgc gcggattgcc gctgccggct ccttcgaggt gtcgatgagg tcctcggtga
     3721 gcgcgccgat gatgccgtcg cgcccggcgt aaaccctgcc gatgcggttc cggtgcttgc
     3781 gcgcggtctc gatgacgccg caggcggtgg tgttgatggt ggcggtcacg cccccggact
     3841 gggcgtaaaa ggcattcttc tgggtcatac ggtaaataaa acagccacag agttcacgca
     3901 ggaaaaccgg acttcaggag gggcgaattt taccatcaac agaacgtgtt gcgccgcccc
     3961 cgcgcaagtc cggtcggctc cgtgccctcg gtggcttgcc gtctatttct tgctctgggc
     4021 gagcgcggcg aacatccggt cgcggatttc ctcgaccttg ccggtgccgg gcaatttgat
     4081 aacgcgtggc gcgcgcttgt cgccgttcgc cgcccatttc gagtaaaagg cgatcaatgg
     4141 ctgcgtctgg gaatggtaat tggcgatgcg ccgcttcacc gtcgcctcgt tgtcatcggc
     4201 ccgctgcacc aggtcctcgc ccgtcacgtc gtccttgccc ggcacccggg gcgggttgaa
     4261 ggtgacgtga taggtcctgc ccgaggcggg atgcacacgc cgcccgctca tgcggcgcag
     4321 gatctcgtcg tcgccgacgg cgatttccac gacgtaatcg atgtcgatgc cggaatggcg
     4381 caacgcctcg gcctgcgcga tggtgcgggg aaagccgtcg acgatgaatc cctgcgcgca
     4441 atcggtctcc ttcacgcgct gcttgacgag ctcgatcacg acccggtcgg gaacgagctc
     4501 ccccttgtcc atgtagcccc ccgcttcctt gccgagcggt gtgccggcct tgatcgcggc
     4561 gcgcagcatg tcgcccgtcg agatctgcgg aattctgaac ttgtccatca ggaactgcgc
     4621 ctgggtgccc ttgccggcgc cgggcatgcc cagcaggatt attctcatgt cattctcatg
     4681 gagcgttaac cggtgaatgt atcaccgaac aagatcccgt cacaacgcgc tcgccgccgc
     4741 tattttagct tgcgcagcag gcgcctgagg tcggcaggcg tgtccacgcc gggatgcggg
     4801 acggagcgcg tgaccgcgca gtgaatgcgg atgccgttgg cgagcgcgcg caattgctcg
     4861 agcgcctcga agcgctcgag cgcggtcggc tcgagccggg cgtactgccg cagaaaggcg
     4921 gcccggtagg cgtagatccc gatatggcga tagaccggca gcccggcggg cagcgcgcgg
     4981 atcccgcgcg caaacgcatt ccgggcccag ggaatcggcg cgcggctgaa gtaaagcgcg
     5041 tagcccgcgc cgtcgagcgc gaccttgacg acgttgggat tgcgcatctc gcgcgccgag
     5101 cgcagcggat ggcaggcggt ggcgatgcgg gcgttgcggc tacgcgccag gtccgccgca
     5161 acggcccgga tgagggccgg gctgatgagc ggctcgtcac cctgcacgtt gacgatgatg
     5221 cgatccggcg ggtagcgccg ccggcgcgca acttccgcga ggcggtcggt gccggttgca
     5281 tggcgggccc gggtcatcac cgc
//
LOCUS       DBNGSG010000166         8140 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1243156, whole genome shotgun sequence.
ACCESSION   DBNGSG010000166 DBNGSG010000000
VERSION     DBNGSG010000166.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 8140)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8140)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8140
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1243156"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..1120)
                     /locus_tag="AC158Q_04330"
     CDS             complement(131..1120)
                     /locus_tag="AC158Q_04330"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3339030.1"
                     /translation="MKALRRIALVISALALAGMAHAQDPSTRSGQAWPSKPIRLIVPF
                     TPGAASDVLGRILALRLSDIYAQQVVVDNRPGAGGLIGSDLARHAAPDGYTIAMVGQP
                     HLSNALLRTEKPYDPLKDYTAISLVGSTHNVIVLGKSVNAKTIPDLIALARAKPGALN
                     YGSAGVGSSSHLAGALFAAKAKIDVVHVPFRVGPDSRNALIAGTIHYYVYPLPAIVGM
                     LKAGTLRALAVGSPKRAAALPDVPTSAEAGFGEYRSESWFGLIGPRAMPKPLVARINA
                     DTATVLREPATRDKIVQQGAEPRFGPPEHFAKMQRDEYAELGALIKQIGMKTQ"
     gene            complement(1186..1830)
                     /locus_tag="AC158Q_04335"
     CDS             complement(1186..1830)
                     /locus_tag="AC158Q_04335"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR3339031.1"
                     /translation="MTLTLYFAPMTCALVPFVTLTEAGADFDVRNINTRNGQNRTPEF
                     LRLNPKHKVPVLVIDGEPLTENVALQIWIHRQFPRARLLPDDPNEEIRAISLMAFCAS
                     GIHPHLTPNARPQNYCDLPDSEESVKRVANKLLFEDFKLVDDLLAGREWFFPHFTACD
                     AYFFWCFKRAASFKLDLARFGNCSAHFERMKLRPSVQKVLACEKRLQDEFARAA"
     gene            1898..2869
                     /locus_tag="AC158Q_04340"
     CDS             1898..2869
                     /locus_tag="AC158Q_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339032.1"
                     /translation="MTSMSSGASRRSAAGFIAASVALAGLVYHGGAPASTLNLRVDRV
                     TGIAARADAEFTRFIRATSRARSVVPGPKPLPASPRSASTRADKPVVTAAGVGAGQAG
                     YVHYFIIGMPDDTLEIQVGIELPDQRIAWSFPDLGVAVSPFIESGIVTAGGKDYEIWH
                     LYGIRPFPDDGAMAVLRKELAGRIQPWLKAGIPYCENDGPRANCMSCLGFVLRALFPG
                     RTSDYPALPRDFWRAGSASRYTTRDLLLYLTGMLELPSREARLQRLSRLALPEDLRED
                     VEELVYAMGAFESASTAGADKPQRSAQSRPAARPAKPGIRPGLRRKL"
     gene            complement(2877..3722)
                     /locus_tag="AC158Q_04345"
     CDS             complement(2877..3722)
                     /locus_tag="AC158Q_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3339033.1"
                     /translation="MSGALRGALWMGGAVLSFSAMAIAVRERARTMGSFEILFLRSVV
                     SLAIMAALLPHFGLRALRTQRLGLHVFRNVLHFGGQYAWVYAISLLPLATVFAIEFTM
                     PVWAVVLAMLILGERLNRGRVVMLALGLAGILVILKPGIEPVSPAALVMLAGAFAYAS
                     IMIATKRLAQSDTAFAILFYMAAIQVPLGLFPALREWVAPGLADLPWIVAVGAAGLIA
                     HFCMTRSFRIADASLVVPVDFLRLPLIAVVGVVFYDEPLELSVLLGATLIFAGTYYSI
                     RRESR"
     gene            complement(3775..4875)
                     /locus_tag="AC158Q_04350"
     CDS             complement(3775..4875)
                     /locus_tag="AC158Q_04350"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3339034.1"
                     /translation="MHVRQDIIHQPAGLVFTGCGLTAAARAQARGKAPGKAKAKPAAA
                     RRREVKVGGRRVKTIDVHAHCVIPEAMKLLGLKMEDQRGPAIDQVGERRLREMDEQGI
                     DVEALSINPQWYRAERDLAARVIRIQNEKLAEFCATYPDRFVAFASLALQYPDLAVEQ
                     LVHGVKKLGLRGAAIGGSVAGVEFSDPRFHPVWAKAEELGVLLFIHPQSTPELNNRFK
                     GNGWLANTIGNPLDTTIALSHLIFEGTLDRFPRLNICSAHGGGFLPSYAPRSDNCLRV
                     APEMDTGVKLRKKPTEYLRQMYYDTLVFTSEALRHLAAEVGPDRLVIGTDHPIPWQSK
                     SVDHILKAPGFSDKERRQMLGETAARLLGIKP"
     gene            5112..7094
                     /locus_tag="AC158Q_04355"
     CDS             5112..7094
                     /locus_tag="AC158Q_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3339035.1"
                     /translation="MIRLSQVTLRRGTKVLLEGADVSLNPGDKIGLIGANGSGKSSLF
                     ALLRGELHADLGEVDFPARWRVAHVAQETPALDRPALEYAIDGDTTLRRLERELAAAE
                     AANDGLLIGELHAAMGDADAYTVRSRAEQLLHGLGFSHEEMDEPVASFSGGWRMRLNL
                     AQALMCPSDLLLLDEPTNHLDLDAILWVEDWLKRYGGTLVIISHDRDFLDGVVNVVVH
                     IDNRKLKRYAGNYSDFERQRAAAVVLAAGMIEKQARERAHLESFINRFRAQATKARQA
                     QSRMKMLARMEDLAPLHVSAPFSFEFRDPLKAPDPLLTLDDVDAGYRLEGASARTVVR
                     GIRFSLRSGQRYGLLGINGAGKSTIMKTLAGELEPLAGTATVNKGLAIGYFAQHQVEM
                     LRHDESPLWNLARVAQGEREQALRNFLGGFNFPGEMALGPIENFSGGEKARLALAMIV
                     WSRPNLLLLDEPTNHLDLETREALTVALAQFEGTLVLVSHDRHLLRATTDQFLIVADG
                     RLQPFDGDLDDYRDWLFRTKLGVKEAGAEAAPAPETMPLPEARKAAPGEPAVTPPPAD
                     RRGQRRRDAAERQRLSAARKPLESRLKRLDESIAKLNAQKAAMDKLLADPGLYGDEKK
                     DTLKAAILDQAYVARELAQLEAEWLALHEQLDAVTQ"
     gene            complement(7137..7982)
                     /locus_tag="AC158Q_04360"
     CDS             complement(7137..7982)
                     /locus_tag="AC158Q_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3339036.1"
                     /translation="MEYRKRTVALPSGERVPALGQGTWHMGEDRAARAEELAALRLGL
                     DLGLTLIDTAEMYGEGEAEKLIGEALAGRRDEAFLVSKVYPHNASRKGAAAACERSLK
                     RLRTDCIDLYLLHWRGDVSLAETMEVFTALRQAGKIRHYGVSNLDLADMQELWPVPGG
                     RETQVNQLLYNLTRRGIEWDLLPWLRRQRVPVMAYSPIEQARLVRDPKLAGFARRHGM
                     TPAQAALAWLLAPGDVIVIPKSGRRERMKENAAALEVKLTREQISELDRLYPPPTKPR
                     PLEML"
BASE COUNT         1309 a         2785 c         2743 g         1303 t
ORIGIN      
        1 cagcgaccac cacgtcacgc tcgacaaccc cgccgggttc acgcgcgcgg tgaaatcgtt
       61 cctggcgaga aatccctgag atgcggcggc gggtggcgct tctccctcat ccctccgccc
      121 tcatccctca tcattgggtc ttcatgccga tctgcttgat cagcgcgccc agctcggcgt
      181 actcgtcgcg ctgcatcttg gcgaaatgct cgggcgggcc gaaccgcggc tccgcgccct
      241 gctgcacgat cttgtcgcgc gtggcgggct cgcgcagcac ggtcgcggta tcggcgttga
      301 tgcgcgcgac cagcggcttg ggcatggcgc gcggcccgat caggccgaac cacgactccg
      361 accggtactc gccgaagccc gcttccgcgc tcgtcggcac gtccgggagc gccgcggcgc
      421 gcttcggcga gccgaccgcc agcgccctca gcgtgccagc cttcagcatg cccacgatcg
      481 ccggcagcgg atagacgtag tagtggatcg tgccggcgat cagcgcgttg cggctgtccg
      541 ggccgacgcg gaacggcacg tgcacgacgt cgatcttcgc cttcgcggcg aacagcgcgc
      601 cggcgagatg agatgaactc cccaccccgg ctgagccgta gttgagcgcg ccgggcttgg
      661 cccgcgcgag cgcgatcagg tccgggatcg tcttcgcgtt cacgctcttg ccgagcacga
      721 tcacgttatg ggtcgagccg accagcgaga tcgcggtgta atccttcagg ggatcgtagg
      781 gtttttccgt gcgcaacagc gcgttcgaca ggtgcggctg cccgaccatg gcgatggtgt
      841 agccgtcggg ggcggcgtgc cgcgcgagat cgctgccgat cagcccgccg gcgcccggac
      901 gattgtcgac gacgacctgc tgggcgtaga tgtcgctcag gcgcagcgca aggatgcgcc
      961 cgaggacatc gctcgccgcg cccggcgtga acggcacgat caggcggatg ggtttcgatg
     1021 gccaggcctg tcccgagcgt gtcgaaggat cctgcgcgtg cgccatgccc gccagcgcga
     1081 gagcggaaat taccagtgca atcctgcgta atgctttcat gatctcctcc attgggccgc
     1141 gattttcatc ttttcccttt tgccttttgc cttttgcctt tctcgttacg ccgcccgcgc
     1201 gaactcatcc tgcagccgct tttcgcaggc gagcactttc tgcacgctcg ggcgcagctt
     1261 catgcgctcg aagtgggccg agcaattgcc gaagcgcgcc aggtcgagct tgaacgaagc
     1321 cgcgcgcttg aaacaccaga aaaagtatgc gtcgcacgcg gtgaagtgcg ggaagaacca
     1381 ctcgcggccg gccagcaggt cgtccacaag cttgaaatcc tcgaacagca gcttgttggc
     1441 gacccgcttc acgctctcct ccgaatcggg cagatcgcag taattctggg gccgggcgtt
     1501 gggcgtgaga tgcgggtgga tgccggacgc gcagaacgcc atcagcgaaa tcgcccggat
     1561 ctcctcgttc gggtcgtccg gcagcagcct ggcccgggga aactggcggt ggatccagat
     1621 ctggagcgcg acgttttccg tcagcggctc gccgtcgatg acgagtaccg gcaccttgtg
     1681 cttggggttc aagcgcagga actcgggggt ccggttctgg ccgttgcggg tgttgatgtt
     1741 ccggacgtcg aagtcggcgc cggcctcggt gagcgtcacg aagggcacca gcgcgcaggt
     1801 catgggggcg aagtaaagcg tcagcgtcat ctcgcgttct ccggggggcg ggagggaatt
     1861 ctagcagcac ggcggccggg ccgtgatccg tgcgaaaatg acttccatgt catcaggggc
     1921 ttcaaggcga tcggcggccg gcttcatcgc cgcgtcggtt gcgctggccg gcctcgtgta
     1981 tcacggcggc gcgccggcgt ccacgctgaa cctccgcgtc gaccgcgtca ccgggatcgc
     2041 tgcccgcgcc gatgcggaat tcacgcgctt catccgcgcg acctcgcgcg cccgttccgt
     2101 cgttcccggc cccaagccgc tgcccgcaag cccgcgctcc gcatccacgc gtgccgacaa
     2161 gccggtcgtc accgcggcgg gcgtgggcgc gggtcaggcg ggctacgttc actacttcat
     2221 catcgggatg ccggacgaca cgctggagat ccaggtcggg atcgaattgc ctgatcagcg
     2281 gatcgcctgg tcgttcccgg acctgggcgt cgccgtatcg ccgttcatcg agtccggcat
     2341 cgtgacggcg ggcgggaaag actacgagat ctggcacctc tacggcatcc gccccttccc
     2401 ggacgatggc gccatggccg tgctgcggaa ggaactcgcg ggccgcatcc agccctggct
     2461 caaggcgggt atcccctatt gcgagaacga cggaccgcgc gccaactgca tgagctgcct
     2521 cggcttcgtg ctgcgcgccc tgttcccggg acgaacgagc gactaccccg ccctgccccg
     2581 cgatttctgg cgcgcgggaa gcgcctcgcg atacacgacc cgggatctgc tgctctatct
     2641 caccggcatg ctcgaactcc cgagccggga agcccgcctg caacggcttt cgcggctggc
     2701 gctgccggaa gacctgcgcg aggacgtgga ggagctcgtc tacgcgatgg gcgccttcga
     2761 gtcggcctct acggcgggcg ccgacaaacc acagcgctcc gcgcagagcc gcccggccgc
     2821 ccggcccgcg aaacccggca tccgtcccgg cctgcgccgg aagctgtagc aaggcttcac
     2881 cggctctcgc gccggatgct gtaataggtc ccggcaaaga tcagcgtcgc ccccagcagc
     2941 accgagagct cgagcggctc gtcgtagaaa accacgccga ccacggcgat cagcggcagc
     3001 cggagaaaat cgaccgggac gaccagcgaa gcatcggcta tgcggaagga gcgggtcatg
     3061 cagaaatgcg cgatcagccc cgccgcgccg accgccacga tccagggcag atcagccagg
     3121 ccgggggcga cccactctct cagcgcgggg aagagtccga gcgggacctg gatggccgcc
     3181 atatagaaca ggatggcgaa cgccgtatcg ctctgggcca ggcgcttggt ggcgatcatg
     3241 atcgaggcgt aggcgaacgc gccggccagc atcaccagtg ccgccggcga caccggctcg
     3301 atcccgggct tcaggatgac gaggatgccg gccagtccca gggccagcat gacgacgcgg
     3361 ccgcggttca ggcgctcgcc caggatcagc atggcgagca cgacggccca taccggcatg
     3421 gtgaactcga ttgcgaacac ggtcgcgagc ggcagcagcg aaatcgcgta tacccacgcg
     3481 tactggccgc cgaagtgcag cacgttacgg aatacgtgca ggccgagccg ctgcgtgcgc
     3541 aacgcgcgca ggccgaagtg cggcaggagc gcggccatga tggcgagcga gaccacgctg
     3601 cgcaggaaca ggatctcgaa gctgcccatg gtgcgcgcgc gctcgcgcac ggcgatcgcc
     3661 atcgcggaga acgacagcac cgcgccgccc atccacaggg cgccgcgcag cgcgccggac
     3721 attgcgcccc ccgcccgtcg atcgtcggcc gtcaccgccc ggccgccggc cgggctaggg
     3781 cttgatgcca agcagcctgg ccgcggtctc gccgagcatc tgccggcgct ccttgtcgct
     3841 gaagcccggc gccttgagga tgtggtccac cgatttgctc tgccacggaa tcgggtggtc
     3901 ggtgccgatg acgagccggt ccgggcccac ttccgccgcc aggtgccgca gcgcttccga
     3961 ggtgaagacc agcgtgtcgt aatacatctg ccgcaggtac tcggtcggct ttttcctgag
     4021 cttcacgccg gtatccatct ccggcgcgac gcgcagacag ttgtccgagc gcggcgcgta
     4081 ggacggcagg aagccgccgc cgtgggcgga gcagatgttc agccggggaa agcggtcgag
     4141 cgtgccctcg aagatcaggt gcgagagcgc gatggtggtg tcgagcgggt tgccgatcgt
     4201 gttcgccagc cagccgttgc ccttgaagcg gttgttcaac tccggcgtgc tctgcgggtg
     4261 gatgaatagc agcacgccca gctcctcggc cttggcccag accgggtgga acctcgggtc
     4321 ggaaaactcc acgccggcga cgctgccgcc gatggcggcg ccgcgcaatc ccagcttctt
     4381 cactccgtgc actagctgct cgacggccag gtccggatac tgcagcgcga gcgacgcgaa
     4441 agcgacgaag cggtccgggt aggtcgcgca gaattccgca agcttctcgt tctggatccg
     4501 gatcacccgg gcggcgaggt cgcgctccgc cctgtaccac tgcgggttga tgctgagcgc
     4561 ctccacgtcg atgccctgct cgtccatctc gcgcaggcgc cgctccccca cctggtctat
     4621 tgccggcccg cgctggtctt ccatcttcaa tcccagcagc ttcatggctt ccggtatcac
     4681 gcagtgcgcg tggacatcga tggtcttcac gcggcggccg cctaccttca cctcgcggcg
     4741 gcgcgcggcg gccggcttcg ctttcgcctt gcccggcgcc ttgccccggg cctgcgcccg
     4801 tgcggcagcg gtgaggccgc agccggtaaa caccaggccg gccggctgat ggatgatgtc
     4861 ctgacgaacg tgcatattgc ctcccgggtt tttggaagtc gcatactacc tttttttgat
     4921 aatgcgtgat gagcgatggg tagttcagga tcaaggatca agttgcaagg ttcaagctgc
     4981 agggcccata gccccttgcg tctcgtgcct tgtgactcgt acctggattt cccgtatctc
     5041 ggcggctcgc cgcgaccgcc cgaaaacggg cggttgcggg tacaatgcgc gcctcgcagc
     5101 gcaggaaccc gatgattcgt ctctcgcaag tgactctcag gcgcggcacc aaggtgctgc
     5161 tcgagggcgc cgacgtctcc ctcaatcccg gcgacaagat cggcctgatc ggcgccaacg
     5221 gctccggcaa gtcgagcctg ttcgcgctgc tgcgcggcga gctgcacgcc gacctgggcg
     5281 aagtggattt tccggcgcgc tggcgcgtcg cgcacgtggc gcaggaaacc ccggcccttg
     5341 accggccggc gctcgagtac gcgatcgacg gcgacaccac gctgcgcagg ctggagcggg
     5401 aactcgccgc cgccgaagca gcgaacgatg gtcttctgat cggcgagctt cacgccgcga
     5461 tgggcgacgc ggacgcgtac accgtgcgct cgcgcgcgga gcagctcctg catggcctcg
     5521 gcttctcgca cgaagagatg gatgagccgg tggcaagctt ctccggcggc tggcgcatgc
     5581 ggttgaacct cgcgcaggcg ctgatgtgcc cgtccgatct gctgttgctg gatgagccga
     5641 ccaaccacct cgatctcgac gcgatcctgt gggtcgagga ctggctgaag cgctacggcg
     5701 gcacgcttgt catcatctcg cacgaccgcg acttcctcga cggcgtggtc aacgtcgtgg
     5761 tgcacatcga caacaggaag ctgaaacgct acgccggcaa ctactcggac ttcgagcgcc
     5821 agcgcgcggc cgcggtggtg ctcgcggcgg gcatgatcga gaagcaggcg cgcgagcggg
     5881 cgcacctgga gtcgttcatc aaccgcttcc gggcccaggc caccaaggcg cggcaggcgc
     5941 aaagccgcat gaagatgctg gcgcggatgg aggacctcgc gccgctgcac gtttccgctc
     6001 ccttctcgtt cgagttccgc gaccccctga aggcgcccga ccccctgctc acgctggacg
     6061 acgtggatgc cggctaccgg ctggaaggcg caagtgcccg gacggtcgtg cgcggcatcc
     6121 gcttctcgct gaggagcggc cagcgctacg ggctgctcgg catcaacggc gccggcaagt
     6181 cgacgatcat gaaaaccctc gccggggagc tggagccgct cgccggcacg gcgaccgtca
     6241 acaaggggct cgccatcggc tatttcgcgc agcaccaggt ggagatgctg cgccacgacg
     6301 agtcaccgct gtggaatctc gcgcgcgtcg cgcaaggcga aagagaacag gcactacgca
     6361 attttctcgg cggcttcaat ttcccgggcg agatggcgct gggcccgatc gagaatttct
     6421 ccggcggcga gaaagcacgg ctcgcgctcg ccatgatcgt gtggagccgc cccaacctgc
     6481 tgctgctgga tgagccgacc aatcacctcg acctggagac gcgcgaggcg ctgacggtgg
     6541 cgctggcgca gttcgagggc acgctggtgc tggtgtcgca cgaccgccac ctgttgcgcg
     6601 ccaccaccga ccagttcctg atcgtcgccg acgggcggct gcagccgttc gacggcgacc
     6661 tcgacgacta ccgcgactgg ctgttcagga ccaagctcgg cgtgaaggag gcgggggccg
     6721 aggccgcccc ggcgccggaa acgatgccgc tgcccgaggc gaggaaggca gcgccgggcg
     6781 agcccgccgt cacgccgccg ccggctgacc ggcgcgggca gcggcgcagg gacgcggcgg
     6841 agcgccagcg tttgtccgcc gcgcgcaagc cgctcgaatc gcgcctgaag cggctggacg
     6901 agagtatcgc caagctgaac gcgcagaaag ccgcgatgga caagctgctc gcggaccccg
     6961 gcctgtacgg cgacgagaag aaggacacgt tgaaggcggc gatcctcgac caggcctacg
     7021 tcgcgcgcga gctggcgcag ctcgaggcgg aatggctcgc gctgcacgag caactggatg
     7081 ccgtgacgca gtaatgcggt ggccgggcgc gaacgtccgg ctcgcagcac ggcggatcag
     7141 agcatttcca gcgggcgcgg tttcgttggc ggcgggtaca gtcgatcgag ctccgagatc
     7201 tgctcccgcg tcagttttac ctcgagcgcg gccgcgttct ccttcatccg ctcgcggcgg
     7261 ccgctcttcg gtatgacaat gacgtcgcca ggcgcgagca gccaggcgag cgcggcctgc
     7321 gccggcgtca tgccgtggcg ccgggcgaaa ccggcgagct tggggtcgcg cacgagccgc
     7381 gcctgctcga tcggagaata ggccatgacc ggcacgcgct gccggcgcag ccagggcagc
     7441 aggtcccact cgatgccgcg gcgcgtgagg ttgtagagca gctggttcac ctgcgtctcc
     7501 cggcccccgg gcaccggcca cagttcctgc atgtccgcga gatccaggtt gctcacgccg
     7561 tagtggcgga tcttgcctgc ctgcctgagc gcagtgaaaa cctccatcgt ctcggcgagc
     7621 gacacgtccc cgcgccaatg caggaggtag agatcgatgc agtcggtgcg cagccgcttc
     7681 aggctgcgct cgcaggccgc ggcggcgccc ttgcgcgacg cattgtgagg atagaccttg
     7741 ctcacgagga acgcctcgtc gcgccggccc gcgagcgcct cgccgatcag tttctcggcc
     7801 tcgccctcgc cgtacatctc ggcggtgtcg atcagggtaa ggccgagatc cagtcccagc
     7861 cgcagcgcgg caagctcttc cgcgcgggcc gcccggtcct ctcccatgtg ccaggtgccc
     7921 tggcccagcg ccggcacgcg ctcgcccgac ggcagcgcca ccgtcctctt gcggtattcc
     7981 atcaccgtag gatatcgctt ccggccgccc gccagccgcc ggagaaccgt gattccgccc
     8041 tttcctccag gcgcccggca gtcgtaccat ggggcctgcg cgaaatggag agtgcaatga
     8101 gcatccgaac cgatccaaaa accctgggca tcgccgccgt
//
LOCUS       DBNGSG010000167         1838 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1754095, whole genome shotgun sequence.
ACCESSION   DBNGSG010000167 DBNGSG010000000
VERSION     DBNGSG010000167.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1754095"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..1239)
                     /locus_tag="AC158Q_04365"
     CDS             complement(169..1239)
                     /locus_tag="AC158Q_04365"
                     /inference="COORDINATES: protein motif:HMM:NF017263.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HIR3339037.1"
                     /translation="MCPSSATLHGSLAVVLLAALAGSAPAVAADAPLRSPFRIPDPAL
                     SSSAAAVTCAQPPPAQRNLRAVRYYSDAAGTVEDPALRRQNDTALARVRGYVRELTRL
                     TDRYRRSGGARAAECALDWLDDWAQSKALLGTTNGNQAEYERKWALAALALNYLKIRD
                     AVSLDTEKLRRVENWLSELAAVVVRFHENPRRGRNNHLYWSGLAVAASGVAANDARLF
                     RWGMAVYHEALGQIRDDGTLPLELARKELALHYHNFAAAPLALIAEIGEANGIPLYDA
                     GGGAFHRLVRRTLEGMRDPAYFQHLTGAAQRVPRGGIMAWLEPYAARFPSSPAARLAA
                     ELRPLGYDRLGGDLTLLFAAGG"
     gene            complement(1241..>1838)
                     /locus_tag="AC158Q_04370"
     CDS             complement(1241..>1838)
                     /locus_tag="AC158Q_04370"
                     /inference="COORDINATES: protein motif:HMM:NF014659.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HIR3339038.1"
                     /translation="IVLVAAMLSAVPLAGAAESMANAIFLVAKPSLADPNFRETVVLV
                     THPRRGGPWGVIINRPLEHRLSEALPDIEALQGRKDVLFYGGPVKREGLVFLVRLSKA
                     PPGATLVLRDVFFTADTGLIDGLLKRPNPTHGLRVYSGFSGWSSGQLQRELARGSWYI
                     LPADAETIFDKDPARVWPELVKRAATRQTRLDVPAIAH"
BASE COUNT          316 a          645 c          616 g          261 t
ORIGIN      
        1 cgccgcgccc ggcaatcgcc accacccgcg cgcggcccag gtcgccgcgc cggatgtgcc
       61 cgatgaacag ccgcgcaatg ctgccgccgc cgatgattgc cacccgcatg acgactccag
      121 acgcagaaca gaaaagttat tttacagggt ggggccgata cgggcatttc agccgcccgc
      181 cgcaaacagc agcgtcaggt cgcctcccag acggtcgtag ccgagcggcc tcagctcggc
      241 ggccagtcgt gcggccggcg aggacgggaa gcgcgcagca tagggctcca gccacgccat
      301 gatgccgccg cgcgggacac gctgagcagc gccggtcagg tgctggaaat aggccgggtc
      361 gcgcatgcct tccagcgtgc gccgcacgag ccggtgaaat gcgccgccgc cggcgtcgta
      421 gagcgggatc ccgtttgctt cgccgatctc ggctatcagt gcaaggggcg ccgcggcgaa
      481 gttgtggtaa tggagcgcga gttccttgcg cgcaagctcg agcggcaggg tcccgtcgtc
      541 gcgtatctgg cccagcgctt cgtggtacac cgccatcccc cagcggaaca ggcgcgcgtc
      601 gttggccgcc acgccgctgg cggcgaccgc gagtcccgac caatacaggt gattattgcg
      661 gccgcgccgc ggattctcat ggaagcgaac caccactgcc gcgagttcgc ttaaccagtt
      721 ctcgacacgc cgcagcttct ccgtatcgag cgacacggca tcgcgtatct tcaggtagtt
      781 gagcgccagc gcggccagcg cccacttgcg ctcgtactcg gcctggttgc cgttcgtggt
      841 gccgagcaga gccttcgatt gcgcccagtc atccagccag tccagggcgc actcggcagc
      901 gcgcgcgccg ccggaacgcc ggtaacgatc cgtcaggcgc gtcagctcgc gcacataacc
      961 acgcacccgc gccagcgccg tgtcgttctg cctccgcaac gccggatcct ccaccgtgcc
     1021 ggcagcgtcg gagtaatagc gcactgcccg caagttgcgc tgcgccggcg gcggttgcgc
     1081 gcacgttacg gcagctgccg acgatgacag cgccggatcg gggatccgga acgggctgcg
     1141 aagcggcgca tcggcggcga cagcgggcgc ggagccggcc agcgcggcaa gcagcacgac
     1201 agccaggctg ccgtgcagcg tcgcggatga ggggcacaca tcaatgcgcg atggccggaa
     1261 catcgaggcg cgtctgcctg gtggcggcgc gcttcaccag ctccggccag acgcgcgccg
     1321 gatccttgtc gaagatcgtt tccgcatccg ccgggagtat gtaccagctg ccgcgcgcga
     1381 gttcgcgctg cagctggccg gaagaccacc ctgaaaaacc cgagtaaacg cgcaggccgt
     1441 gcgtcggatt ggggcgcttc agcaggccgt cgatcaaccc ggtatcggcc gtgaagaaga
     1501 cgtcgcgcag cacgagggtc gcgcccggcg gcgccttcga cagccgcacc agaaacacca
     1561 ggccctcgcg cttgaccggt ccgccgtaaa aaagcacgtc cttgcggccc tgcagcgctt
     1621 cgatatcggg cagcgcctcg ctcaaccggt gttcgagcgg gcggttgatg atgacgcccc
     1681 agggaccacc ccgccgcggg tgcgtcacca gcacgacggt ctcgcggaaa ttggggtcgg
     1741 ccaggctggg cttcgcgacg aggaatatcg cgttcgccat gctttccgcg gcgcccgcca
     1801 acggcaccgc ggaaagcatg gcagccacca gcacaata
//
LOCUS       DBNGSG010000168         8590 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1243829, whole genome shotgun sequence.
ACCESSION   DBNGSG010000168 DBNGSG010000000
VERSION     DBNGSG010000168.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 8590)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8590)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8590
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1243829"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(266..1708)
                     /gene="fumC"
                     /locus_tag="AC158Q_04375"
     CDS             complement(266..1708)
                     /gene="fumC"
                     /locus_tag="AC158Q_04375"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004532308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HIR3339039.1"
                     /translation="MAKQKVRIERDTMGPIAVPADRLWGAQTQRSLHHFHVPGETMPL
                     EVVRAQVLVKKAAAATNMALGVLDRKKGAAIVKAADQALRGRLDDHFPLVVWQTGSGT
                     QTNMNVNEVLANRASEMLGGSRGERRLVHPNDDVNKGQSSNDTFPTAMHVAAVIAIER
                     NLKPAVRRLRNTLDRKAKEFAKIVKIGRTHLQDATPLTLGQEFSGYVSQLDHGLAHVD
                     AACPHLCELALGGTAVGTGLNAHPQFAEKVAAEIARLTRLPFVTAPNKFEALASCDGV
                     VHAHGALKTLAASLMKIANDIRWLASGPRSGIGELIIPENEPGSSIMPGKVNPTQSES
                     MTMACCQVLGNDVAVNLGGASGNFELNVFRPLVIRNFLHSARLLARACENFNEHCAVG
                     IKPNRARIEQLMRDSLMLVTALNPHIGYEKAAAIAKKAHKEGTTLKEAAIKLGYVTPK
                     QFDAWVRPEKMVGNVEISRQDAKTPRKTKR"
     gene            complement(1828..4122)
                     /locus_tag="AC158Q_04380"
     CDS             complement(1828..4122)
                     /locus_tag="AC158Q_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HIR3339040.1"
                     /translation="MDEQLRKAALDYHRLPTPGKISVAPTKSLVNQRDLALAYTPGVA
                     IACMEIAANPAEARNLTARGNLVAVITNGTAVLGLGAIGPLASKPVMEGKAVLFKKFA
                     GIDVFDIEVDERDPDKLVDIIASLEPTFGGINLEDIKAPECFIVEKKLRARMKIPVFH
                     DDQHGTAIIVGAAILNGLKVVGKDIGSVKLVVSGAGAAALACLNLLVDMGMRRSNIVV
                     TDIKGVVYKGRKDEMDPEKERFAVETRARTLGEVIDGADVFLGLSAGGVLKPDMVGRM
                     SARPLILALANPEPEIRPEEVMKVKPDAVMATGRSDYPNQVNNVLCFPFIFRGALDVG
                     ATTITREMEVAAVKAIAELAQAEQSDIVAMAYGEQNLTFGPEYLIPRPFDPRLIAKIA
                     PAVARAAMESGVATRPIADFDAYAERMNQFVYQSGLIMKPIFAAAKDAPKRVVYCEGE
                     DERVLRAVQQVVDEGIARPTLVGRREVVRMRIERLGLRLKPDADYDLVDPNNDPRYRE
                     YSTAYHALAQRKGVTPQIARLEMRRRYTLIGVMLMSMREVDAMLCGVVGRYDSHLRFV
                     DHVIGLRPGVKNYAAMNALLLPNRTVFICDTYVNYDPTAEQIAEMTVLAAEEIRRFGI
                     TPKAALLSHSSFGAAGTPTAVKMRQALELIENLAPGLEIEGEMQGDAALSEEIRDRVF
                     PNARLKGGANLLVMPTLDAANIAFNLLKTAAGDGITVGPILLGVARPVNILTPSATVR
                     RIVNMTALTVVDAAAQRGEQRALL"
     gene            4299..5267
                     /gene="pip"
                     /locus_tag="AC158Q_04385"
     CDS             4299..5267
                     /gene="pip"
                     /locus_tag="AC158Q_04385"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004554735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HIR3339041.1"
                     /translation="MADISHTPRVELYPEIEPYSAGMLKLDGVHSMYWEQSGNPEGAP
                     VLFLHGGPGAGASPAHRRFFDPAHYRIVIFDQRGAGRSTPLGELRDNTTPRLVADIER
                     LRAHLGIDRWLVFGGSWGSTLALAYGEAHPERCTGFILRGVFLCRASEIEWFLYGLRN
                     LFPEAWRTFAETIPAAERGDLLSAYYRRLTDPDPGVHLPAARAWSTYEGVCSTLLPSP
                     ETVAYFAGDGVSLGLARIEAHYFRHDIFLPQNALLENAHRLRGIPCVIVQGRYDAVCP
                     IVSADDLSRAWPEAEYIIVPDAGHSAWEPGISAELVKAAEKMKRPR"
     gene            complement(5383..6354)
                     /locus_tag="AC158Q_04390"
     CDS             complement(5383..6354)
                     /locus_tag="AC158Q_04390"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003275776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - quinone reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase family protein"
                     /protein_id="HIR3339042.1"
                     /translation="MPYAMRIYETGGPEKLRWEEVQVGAPGEGQVRVRNTAVGLNFID
                     TYQRSGLYPLPLPFTLGSEGAGVVEAVGPKVREFKVGDRVAYSGPLGAYAEVLLRPAE
                     RLVKIPSGVDDKTAAAMMLKGLTAQYLIRRTYRVRKGDTILMHAAAGGVGQILCQWAK
                     ALGATVIGTVGSDEKAALAKKAGCKHVIVTSREKFPERVKEITKGKGVPVVYDGVGKD
                     TFMGSLDCLAPLGLMASFGNASGAVTQFNPGVLAQKGSLFLTRPTLNTYTATRADLVK
                     AAAELFSVVKSGKVKISINQTYPLREAAQAQRDLEARKTTGSTVLIP"
     gene            complement(6490..6903)
                     /locus_tag="AC158Q_04395"
     CDS             complement(6490..6903)
                     /locus_tag="AC158Q_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339043.1"
                     /translation="MATTVRKTAYFSMKTPNRAGQGARLLNALAAHKVNLLAFTGFPN
                     AGRAQVDFIPYDASRFTRAARKLGLKVSKRKTVFLAQGTDKAGAIAGVCGKLAKAGIN
                     MVAMDAVAAGGGRYGAIFWVKPRDIARATRILRAR"
     gene            7100..7993
                     /locus_tag="AC158Q_04400"
     CDS             7100..7993
                     /locus_tag="AC158Q_04400"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA dioxygenase family protein"
                     /protein_id="HIR3339044.1"
                     /translation="MDLPGAKRITVVPTGAALGADIAGVDLSRPLDNDTFRAVEAAWH
                     QHLVLRFRGQNLDDPGLLAFARRFGELDKAPVHAVADSDHDPYPEITVMSNIKLDGKP
                     IGNLGHYEAEWHTDMSYNERTPIGSLLYSLEVPPEGGDTGFSNMYLAFETLPAALKRA
                     ITGKSCKHDSSRNSVGDLRKGFQDVSDPREAPGAVHPIVRTHPATRKNALFLGRRRNA
                     YIVGLPLEESEDLLNRLWAHASKPEFAWYQKWKVGDLIMWDNRSAMHRRDAFDPNSRR
                     LMHRTQIKGDKPFFDPATLAA"
     gene            complement(8108..>8590)
                     /locus_tag="AC158Q_04405"
     CDS             complement(8108..>8590)
                     /locus_tag="AC158Q_04405"
                     /inference="COORDINATES: protein motif:HMM:NF043076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhaM family polyhydroxyalkanoate granule
                     multifunctional regulatory protein"
                     /protein_id="HIR3339045.1"
                     /translation="FAVPRRAAGFGQDAVVGIVEPHRFQKFHEAFRVVFGERGLNGES
                     VVRSNPHCWSGGTVVRSNPIIGVVGSCRPGDDMAQEPELPKDWLEFMQKMWNPMSFPM
                     PGMAMPTVNVEEVEKKIGELKAVETWLTLQVGLLQMTVKTLEMQKAALESMTAGAKAK
                     "
BASE COUNT         1381 a         2874 c         2725 g         1610 t
ORIGIN      
        1 ccgccaagac gccaagcccg ccaagaatac aaataagcaa taattagttt gttccaggcg
       61 accgtcaccc ggcaaagaaa agtatatacc ggtggtgaga aatcactcgg tagtgataaa
      121 tcaccagcca tagcgaacat gggagttctt gcttttctct atgttctctg tgctctctgt
      181 ggcaaatcgc ttttgacttt atctgcgcgt acctgcgttt atctgtggtt ccattatgtt
      241 tttcttggcg gccttggcgg ttctattatc gctttgtctt tcttggcgtc ttggcgtctt
      301 ggcggctaat ttcaacgttt ccaaccatct tctccggacg cacccacgca tcgaactgct
      361 tgggcgtgac atagccgagt ttgatggccg cctccttcag cgtcgtgcct tccttgtgcg
      421 ccttcttggc gatcgccgcc gctttctcgt agccgatgtg gggattcagc gccgtcacca
      481 gcatcagcga atcgcgcatc agctgctcga tgcgcgcgcg gttgggcttg atgccgaccg
      541 cgcaatgctc gttgaagttc tcgcaggcgc gcgcgagaag ccgcgcgctg tgcaggaaat
      601 tgcggatgac gagcggccgg aacacgttca gctcgaagtt gccggaggcg ccgcccagat
      661 tgaccgcgac gtcgttaccg agcacctggc agcacgccat ggtcatggac tccgactgcg
      721 tggggttgac cttgcccggc atgatcgaac tgcccggctc gttctccggg atgatgagtt
      781 cgccgatgcc gctcctcggc cccgaggcga gccagcggat gtcgttcgcg atcttcatga
      841 ggcttgccgc gagcgtcttc agcgcgccgt gcgcgtgcac cacgccgtcg caggaggcga
      901 gcgcctcgaa cttgttgggg gccgtgacga acggcagccg ggtgagccgc gcgatctccg
      961 cggccacctt ctccgcgaac tgcgggtggg cattcaggcc ggtcccgacc gcggtgccgc
     1021 ctagcgccag ctcgcagaga tgcgggcacg ccgcgtccac gtgcgcgagc ccgtggtcga
     1081 gctgcgagac atagcccgag aattcctgcc cgagcgtcaa cggcgtcgcg tcctggagat
     1141 gggtgcggcc gatcttcacg atcttcgcga actctttcgc cttgcggtcg agcgtgttgc
     1201 gcagccgcct gaccgcgggc ttgagattgc gctcgatcgc gatcaccgcc gcgacgtgca
     1261 tcgcggtggg gaaagtatcg ttggaagact gccccttgtt gacgtcgtca ttgggatgga
     1321 ccagccgtct ctcgccgcgg ctgcctccca gcatttccga ggcgcggttc gccagcacct
     1381 cgttgacgtt catattggtc tgggtgccgg acccggtttg ccagaccacc agtggaaaat
     1441 ggtcgtcgag ccgcccgcgc agtgcctggt ccgccgcctt gacgatggcc gcgcccttct
     1501 tgcggtcgag cacgcccagc gccatgttgg tggccgcggc ggctttcttg acgagcacct
     1561 gcgcccgcac gacctcgagc ggcatggtct cgccggggac atggaaatga tggaggctcc
     1621 tctgcgtctg cgccccccac agccggtcgg cgggcaccgc gatcggcccc atcgtgtcgc
     1681 gctcgatacg gactttttgc tttgccataa aatctcctta gccgccgatg aacgccgatg
     1741 aacgcagata aggcaaggcc cgtttcggga tttgttgaat tatcggcgtt tatctgcgtt
     1801 aatctgcggt ttcctggcag ttaggtttta gagcagcgcg cgctgctcgc cgcgctgcgc
     1861 cgcagcgtcc accacggtga gtgccgtcat gttgacgatg cggcgcacgg tggcggaagg
     1921 ggtgagtatg ttcacgggac gcgcgacgcc gagcaggatg ggcccgacgg tgataccgtc
     1981 cccggccgcg gtcttcaaca ggttgaaagc gatgttggcg gcgtccagcg tcggcattac
     2041 cagcaggttc gcgccgccct ttaaccgcgc gttgggaaat acccgatcgc gaatttcctc
     2101 cgatagcgcg gcatcaccct gcatctcccc ctcgatctcg aggcccggcg cgagattctc
     2161 gatcaactcg agcgcctggc gcatcttgac cgcggtcggc gtgccggccg cgccgaagct
     2221 ggagtgcgac agcagcgccg ccttgggcgt gatgccgaag cgccggattt cctcggccgc
     2281 aagtaccgtc atctcggcga tctgttccgc ggtggggtcg tagttgacgt acgtgtcgca
     2341 gatgaagacg gtgcggttgg gcagcagcag cgcgttcatc gcggcgtaat tcttcacgcc
     2401 cggccgcaga ccgatgacgt gatcgacgaa gcgcaggtgg ctgtcgtagc gcccgacgac
     2461 gccgcacagc atcgcgtcca cttcgcgcat ggacatgagc atgacgccga tcagcgtgta
     2521 gcgccggcgc atctccaggc gcgcgatctg cggcgtcacg ccctttctct gcgcgagggc
     2581 gtgatacgcg gtcgagtatt cgcggtagcg cggatcgttg ttggggtcca ccagatcgta
     2641 gtccgcgtcc ggcttgagcc tcagcccgag gcgctcgatg cgcatccgga cgacttcccg
     2701 gcgtccgacg agcgtcgggc gggcaatgcc ctcgtccacc acctgctgga cggcccgcag
     2761 cacccgctcg tcctcgccct cgcagtacac cacgcgcttg ggcgcgtcct tggccgccgc
     2821 gaagatcggc ttcatgatga ggccggactg gtagacgaac tggttcatgc gctcggcgta
     2881 agcgtcgaaa tcggcgatcg gccgcgtggc gacgccggac tccatggcgg cgcgcgccac
     2941 cgccggcgcg atcttcgcga tcaggcgcgg gtcgaacggc cgcggaatga ggtattcggg
     3001 tccgaaagtg aggttctgct cgccgtaggc catcgcgacg atgtccgact gctcggcttg
     3061 cgccaactcg gcgatcgcct tcacggcggc cacctccatc tcgcgcgtga tggtggtggc
     3121 gccgacgtcg agcgcgccgc ggaagatgaa cgggaagcac agcacgttgt tgacctggtt
     3181 cgggtagtcg gaccggccgg tcgccatgac cgcatcgggc ttcactttca tcacttcttc
     3241 cggccggatt tccggctcgg gattggcgag ggcgagtatc aatgggcgcg cgctcatccg
     3301 cccgaccatg tcgggtttca gcacgccgcc cgcggacagc cccaggaaaa cgtcggcgcc
     3361 gtcgatcacc tcgcccagcg tccgtgcccg ggtttcgacc gcgaaacgct ccttctcggg
     3421 gtccatttcg tccttgcgcc ccttgtagac cacgcccttg atgtcggtga cgacgatgtt
     3481 cgagcgccgc attcccatgt cgacgaggag attcaggcag gcgagcgccg ccgcgccggc
     3541 gccggagacg acgagtttca cgctcccgat gtccttgccg accaccttga gcccgttgag
     3601 aatcgcggcg ccgacgatga tggcggtgcc gtgctggtcg tcgtggaaca ccggaatctt
     3661 catgcgggcg cgcagcttct tctccacgat gaagcattcc ggcgccttga tgtcctcgag
     3721 gttgatgccg ccgaacgtgg gctcgaggct ggcaatgatg tccaccagct tgtccgggtc
     3781 gcgctcgtcc acctcgatgt cgaagacgtc gatgccggcg aatttcttga acagcaccgc
     3841 cttgccttcc atgaccggct tggaggcaag cgggccgatc gcgcccaacc ccagcaccgc
     3901 cgtgccgttg gtgatcaccg cgaccaggtt gccgcgggcc gtcaggttgc gcgcctccgc
     3961 cggattggcc gcgatttcca tgcaggcgat cgccacgccc ggcgtgtagg ccagcgcaag
     4021 atcgcgctgg ttgacgaggc tcttggtggg cgcgaccgaa atcttgccgg gagtcggcag
     4081 ccggtggtaa tcgagcgctg ccttgcgtag ctgttcgtcc atgcgatcgt ccagtggaaa
     4141 gcgcgcgttg agcggcacga cgtgtccgcc ccaacgcgcg cagccggccc ggtgcggcgc
     4201 atccgggatt ggcggaaatt atagcgcgtt cgacccgccg ccctattccg ccggtgcgca
     4261 tggctgtgct atgttttgcg ttcgctgcag cccgccttat ggccgacatt tcccacacac
     4321 cgcgcgtgga gctctatccc gagatcgagc cctacagcgc gggcatgctg aagctggacg
     4381 gcgtgcactc catgtactgg gagcagtcgg gcaacccgga aggtgcgccg gtgctgttcc
     4441 tccacggcgg cccgggcgcc ggcgcctcgc ccgcccaccg gcgcttcttc gacccggcgc
     4501 actaccgcat cgtcatcttc gaccagcgcg gcgccggccg ttccacgccg ctcggcgagc
     4561 tgcgcgacaa caccacccct cgcctcgtcg cggatatcga gcgcctgcgc gcccacctgg
     4621 ggatcgatcg ctggctggtg ttcggcggct cctggggcag cacgcttgcg ctcgcctacg
     4681 gcgaggcgca cccggagcgc tgcacggggt tcatcctgcg cggtgtgttc ctgtgccgcg
     4741 cgagcgaaat cgagtggttt ctctacggcc tgcgcaacct gttcccggag gcctggagaa
     4801 cgttcgccga gacgataccc gccgccgagc gcggcgatct tctgtccgcc tattacaggc
     4861 gcctgacgga tcccgacccc ggcgtgcacc tgccggccgc gcgcgcctgg agcacctacg
     4921 agggcgtgtg ctcgacgctg ctgccgagcc cggagacggt cgcctacttt gcgggggacg
     4981 gcgtgtcgct cggcctcgcg cgcatcgaag cgcattactt ccgtcacgac atcttcctgc
     5041 cgcagaacgc gctgctcgag aacgcgcacc ggctgcgcgg cataccctgc gtcatcgtgc
     5101 aggggcgcta cgatgcggtg tgcccgatcg tcagcgcgga tgacctctcc cgcgcgtggc
     5161 ccgaggccga gtacatcatc gtgcccgacg ccggtcactc cgcctgggaa cccggcatca
     5221 gcgccgagct ggtgaaagcc gccgaaaaga tgaaacgtcc cagataaaac gtctcagata
     5281 aagcgtcgca gataaggcgc tgttggacgc cgtttctgag acgcaatacc tgagatgcag
     5341 tacctgagac gccttacctg agacgtatta tctgagacgc cgttacggaa tcaacaccgt
     5401 tgatcccgtc gtcttgcggg cctcgaggtc gcgctgcgcc tgcgccgcct cgcgcagcgg
     5461 gtaggtctgg ttgatcgaga ttttcacctt cccgctcttc accaccgaga acaattccgc
     5521 cgccgccttc accaggtccg cacgggtcgc ggtataggtg ttgagcgtcg ggcgcgtgag
     5581 gaacagggag cctttttgcg ccagcacgcc ggggttgaat tgcgtcacgg ccccggatgc
     5641 gttgccgaag ctcgccatca ggccaagcgg tgcgaggcag tcgagcgatc ccatgaaggt
     5701 gtccttgccg acgccgtcgt agaccaccgg cacgcccttg cccttggtga tttccttgac
     5761 gcgctccgga aatttctcgc gcgaggtgac gatgacgtgc ttgcagccgg ccttcttcgc
     5821 gagcgcggcc ttctcgtcgc tgcccaccgt gccgatcacg gtcgcgccga gcgctttcgc
     5881 ccattggcac aggatctgcc cgacgccgcc ggccgccgcg tgcatgagga tggtgtcgcc
     5941 tttcctgacg cggtaggtgc ggcggatcag gtactgggcg gtgagccctt tcagcatcat
     6001 cgcggcggct gtcttgtcgt caacgccgga aggaatcttc accaggcgct cggccggacg
     6061 cagcagcact tcggcgtagg cgccgagcgg gccggaataa gccacgcggt cgccgacctt
     6121 gaattccctg accttgggcc cgaccgcctc gaccacgccc gctccctcgg accccagcgt
     6181 gaacggcagg ggcagcgggt agagcccgct ccgctggtag gtatcgatga agttcaaccc
     6241 caccgcggtg ttgcgcaccc gcacctgacc ctcgcccggc gcgccgacct gcacctcttc
     6301 ccagcgcagt ttttccggcc cgccggtctc gtagattctc atcgcgtatg gcatcgattt
     6361 ctcctctggc tgaaattcaa agctgcagat tagaacatat ttcggcgcgg ccaagagaac
     6421 gggggcattc cggcccccgt tttcccttcc cggcccggct ccgccggaat cccgggcagc
     6481 tcgatccgtc taacgcgcgc gcagtatgcg cgtcgccctg gcgatatcgc gcggcttcac
     6541 ccagaaaatc gcgccataac gccccccgcc ggcggcgacg gcatccatcg ccaccatgtt
     6601 gatgcctgcc ttggcaagct tgccgcagac gccggcgatc gcgccggcct tgtcggtgcc
     6661 ctgggcgagg aataccgttt tgcgcttgct tactttcagg ccgagcttgc gcgcggcacg
     6721 ggtgaaccgg gaagcatcgt agggaatgaa atccacctgc gcccggccgg cgttggggaa
     6781 accggtgaag gcgagcaggt tgaccttgtg cgcggcgagc gcgttgagca ggcgcgcgcc
     6841 ctggccggca cggttggggg tcttcatgct gaaataagcg gtcttgcgga cagtcgttgc
     6901 cataactgcc tcctttgaaa agggcacacc cgggacgccg ccggttgagc ggcagatcgc
     6961 ggtccggctc gaaccggcgg actggatccg gcgcttgccg tccggaccgg gaattaacta
     7021 tacgccgccc gccgcggacg cacacttgcg gcggccgtcc gctggcatta gcatcgcccg
     7081 ataaccggag ggacagcaca tggacctgcc tggagccaag cgcattaccg tcgtgccgac
     7141 cggcgccgcc ctgggcgcgg atatcgccgg agtggacctg tcccggccgc tcgacaacga
     7201 caccttcagg gccgtcgagg cggcctggca ccagcacctg gtgctgcgct ttcgcggcca
     7261 gaacctggac gacccgggcc tgctcgcttt cgcgcgccgg ttcggcgagc tcgacaaggc
     7321 gccggtccac gccgtggccg acagcgacca cgatccctat cccgagatca ccgtgatgtc
     7381 gaacatcaag ctggacggca aaccgatcgg caacctcggc cactacgagg cggagtggca
     7441 taccgacatg tcgtacaacg agcgcacgcc catcggcagc ctgctctatt cgctggaagt
     7501 gccccccgag ggcggtgaca ccggcttctc gaacatgtac ctcgcgttcg aaacgctgcc
     7561 ggcagcgctg aagcgcgcca ttaccggcaa gtcctgcaag cacgattcca gccgcaatag
     7621 cgtcggcgat ctgcgcaagg gcttccagga cgtgtccgat ccgcgcgagg cacccggcgc
     7681 cgtgcacccc atcgtgcgca cccacccggc aacgcgcaaa aacgccctct tcctcgggcg
     7741 ccggcgcaac gcctacatcg tcgggttgcc gctcgaggag tccgaagacc tgctgaaccg
     7801 gctgtgggcg cacgccagca agccggagtt cgcctggtac cagaagtgga aagtgggcga
     7861 cctgatcatg tgggacaacc gttctgccat gcatcgccgc gatgcttttg acccgaattc
     7921 acgcaggctc atgcatcgca cccagatcaa gggcgacaaa ccgttcttcg acccggccac
     7981 cctggcagcg tgaccggttg cccgcgcgtt gaacggcggg gcgccgtccg ttcgaacgcg
     8041 cggagttaaa tcggtgctta cggtaaggga accgccgttc ccgtgccgca agcatccgga
     8101 tctaacgcta tttggccttc gcgcccgccg tcatcgattc gagcgcggcc ttctgcatct
     8161 cgagggtctt gacggtcatc tgcagcaagc cgacctgcag ggtgagccag gtttccaccg
     8221 ccttcagctc gccgatcttc ttctccacct cctcgacgtt gacggtcggc atcgccatgc
     8281 cgggcatggg gaacgacatc gggttccaca tcttctgcat gaactcgagc cagtctttcg
     8341 gcagttccgg ttcttgagcc atatcgtctc ctgggcggca gctgccgacc acgcctatta
     8401 tagggttgga gcggacgaca gtgccgccgc tccaacagtg cgggttcgaa cggacgactg
     8461 attcgccgtt caacccgcgc tcaccgaaaa caacacggaa cgcttcatga aatttctgaa
     8521 agcggtgcgg ctcgacgatt ccgacgaccg catcctggcc gaatccggcg gcgcggcgcg
     8581 ggacggcgaa
//
LOCUS       DBNGSG010000169         4245 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_225843, whole genome shotgun sequence.
ACCESSION   DBNGSG010000169 DBNGSG010000000
VERSION     DBNGSG010000169.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4245)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_225843"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1873
                     /locus_tag="AC158Q_04410"
     CDS             <1..1873
                     /locus_tag="AC158Q_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3339046.1"
                     /translation="RLDVVNFISHGITKVPQAAHPKADHEAEAEQEASPGGALDNYTL
                     NLNAMALAGKIDPLIGREKELERVIQTLCRRRKNNPLLVGEAGVGKTAIAEGLARRIV
                     ESDVPEVLKRCHVYALDMGALLAGTKYRGDFEQRLKAVLKQLVDNPNAILFIDEIHTL
                     IGAGAASGGTLDASNLLKPALQTGQLKCIGATTYQEYRGVFEKDHALSRRFQKIDVVE
                     PSVPETILILKGLKSRFEAHHGIKYMPSALTAAAELSARFINDRHLPDKAIDVIDEAG
                     AAQKILPKARQKKIIGKHEIEEIIAKITRVPVQAVSADDRSALKNLDRDLKAVVFGQD
                     KAIDALTSAIRMARSGLGNPQKPIGNFLFSGPTGVGKTEVARQLAYVMGVELVRFDMS
                     EYMERHAVSRLIGAPPGYIGFDQGGLLTEAISKQPYSVLLLDEIEKAHPDIFNILLQV
                     MDHGTLTDNNGRKADFRHVVIVMTTNAGATELTKSSMGFTPSQQSGDEMAEIKRMFTP
                     EFRNRLDAIISFNSLDRDIILRVVDKFLMQLEQQLEEKKVEPAFTDALREFLAKKGFD
                     PQMGARPMARLIQDTIRSALADELLFGRLAHGGKVTVDIGADEKIRLVFAEEPAPVA"
     gene            2038..2379
                     /locus_tag="AC158Q_04415"
     CDS             2038..2379
                     /locus_tag="AC158Q_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3339047.1"
                     /translation="MKPIVFGLGCAIALIATGAAAQAQNPAQARGLAATCFTCHGNEG
                     RSVGNVPPSLAGQDRNYMLQQMKDFKAGKRPATIMHQHAKGYSDEQLELIADYFSKVK
                     PGPGAAAPAAR"
     gene            2390..3658
                     /locus_tag="AC158Q_04420"
     CDS             2390..3658
                     /locus_tag="AC158Q_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3339048.1"
                     /translation="MMLSRREFMKWMAASTGAAAAGCAGTGGGSAGRVVVVGGGYGGA
                     TAARYVKEWAPDIEVTVIERNNIFVSCPISNLVLGGNTQIGNITMGYDGLRKRGVRVV
                     RGEAVAVDAAAKQVRLADGTTLGYDRVIVSPGIDFIYDSIPGLKGADAQNRVLHAWKA
                     GPQTVALRKQLEAMRDGGVFVFHIPMAPYRCPPGPYERVCQVADYFKRAKPRSKIIVL
                     DSNSDITSKKGLFLQSWNGVYKGMIDYRPNSELADVDVKGMTVKLTFDSVKGDVLNVV
                     PPHRAGDIARTTGLITANQRWCGVDWMTMESTAVKGVHVLGDATLSAPAMPKSASMAN
                     QHAKVCAAAVAALIKGQPVNPLPVMMNTCYSFVSGNAAMHVASVHQYDSAQKTIVPVK
                     GAGGVSPSASEIEGAYAWGWARNIWADALG"
     gene            complement(3680..4072)
                     /locus_tag="AC158Q_04425"
     CDS             complement(3680..4072)
                     /locus_tag="AC158Q_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HIR3339049.1"
                     /translation="MSQWLADVVLVVHFAFVLFVVGGLALIWIGAAAGWDWVRNLRFR
                     AAHLAAIGFVALEALIGMWCPLTVWEAALRGTQAEKGFIAQWVHRLLYYDWPEWVFTL
                     LHVGFALLVAATWWWVRPAKKTTAPQRR"
BASE COUNT          816 a         1371 c         1438 g          620 t
ORIGIN      
        1 gcggctcgac gtcgtgaact tcatctcgca cggcatcacc aaggtgccgc aggccgcgca
       61 tcccaaggcc gatcacgagg cggaggccga gcaggaggcc tcgccgggcg gcgcgctcga
      121 taattacacg ctgaacctga atgccatggc gctcgccggc aagatcgatc ccctgatcgg
      181 gcgcgaaaag gaactcgagc gcgtcatcca gacgctctgc cgccggcgca agaacaaccc
      241 gctgctggtg ggcgaggcgg gcgtgggcaa gaccgccatc gccgaagggc tggcgcgccg
      301 catcgtggag agcgacgtgc cggaagtgct caagcgctgc cacgtctacg cgctcgacat
      361 gggggccctg ctggcgggca ccaagtaccg cggcgatttc gaacagcggc tgaaagcggt
      421 gctgaagcag ctggtggaca acccgaacgc gatcctgttc atcgacgaga tccacacgct
      481 gatcggggcc ggcgcggctt ccggcggcac gctcgacgcg tccaacctgt tgaaacccgc
      541 gctgcagacc ggccagctga agtgcattgg cgccaccacc taccaggaat accgcggcgt
      601 attcgagaag gaccatgcgc tctcgcgccg cttccagaag atcgacgtgg tcgagccgtc
      661 cgtgcccgag accatcctga tcctgaaagg gctgaagtcg cgcttcgagg cccaccacgg
      721 catcaagtac atgccgtcgg cgctgacggc cgcggcggag ctctccgccc ggttcatcaa
      781 tgaccggcac ctgccggaca aggccatcga cgtgatcgac gaggccggcg ccgcgcagaa
      841 aatcctgccg aaggccaggc agaagaagat catcggcaag cacgagatcg aggagatcat
      901 cgcgaagatc acgcgcgtgc cggtgcaggc ggtgtcggcg gacgaccgca gcgcgctgaa
      961 aaacctcgac cgcgatctca aggccgtggt gttcgggcag gataaggcga tcgacgcgct
     1021 gacctcggcg atacggatgg cgcgcagcgg cctgggcaac ccgcagaagc cgatcgggaa
     1081 cttcctgttc tcgggaccca ccggcgtggg caagaccgag gtcgcgcgcc agctcgccta
     1141 cgtcatgggc gtggagctgg tgcgcttcga catgtcggag tacatggagc ggcacgcggt
     1201 gtcgcgcctg atcggggcgc ccccgggcta catcggtttc gaccagggcg gattgctgac
     1261 cgaagcgatt tccaagcagc cgtactcggt gctgctgttg gacgagatcg agaaggcgca
     1321 cccggatatc ttcaacatcc tgctgcaggt gatggaccac ggcacgttga ccgacaacaa
     1381 cgggcgcaag gccgatttcc gccacgtggt gatcgtcatg accaccaacg cgggcgccac
     1441 cgagctcacg aagtcctcca tgggcttcac gcccagccag cagtcgggcg acgagatggc
     1501 cgagatcaag cgcatgttca cgccggaatt caggaaccgg ctggacgcca tcatctcgtt
     1561 caactccctc gaccgcgaca tcatcctgcg cgtcgtggac aagttcctga tgcagctcga
     1621 gcagcagctc gaggaaaaga aggtcgagcc ggccttcacc gacgcactgc gggagtttct
     1681 ggcgaagaag ggcttcgatc cgcagatggg cgcgcgcccg atggcgcgcc tgatccagga
     1741 caccatccgc tcggcgctgg ccgacgagct gctgttcggg cgcctcgccc acggcggcaa
     1801 ggtgaccgtg gacatcggcg ccgacgagaa gattcgccta gtcttcgcgg aggaacccgc
     1861 ccccgtcgcg tgagccggcg ccgtttgccg accgaaagcc ccggccccgg ccggggcttt
     1921 ttgcttctgc ttgacgccgg ccgcacgtgg ccagaaactt ataagggcat aaggaaattg
     1981 tccaagcggg agccggaacc gcagctatac ttttcacgaa accagctggg agggggcatg
     2041 aaacctatcg tttttggcct gggctgcgcg atcgcgctga ttgcgaccgg agccgccgcg
     2101 caggcgcaga atccggcgca ggcgcgcggc cttgccgcca cctgtttcac ctgtcacggg
     2161 aacgagggcc gcagtgtcgg caatgtgccg cccagtctcg ccggccagga ccgcaactac
     2221 atgctgcagc agatgaagga cttcaaggcg ggcaagcgcc cggcgacgat catgcaccag
     2281 catgccaagg gctacagcga cgagcaactc gagttgatcg cggattattt ctcgaaagtg
     2341 aagccggggc cgggcgccgc agcgccggcc gcgcggtgag gaggcccaca tgatgctcag
     2401 cagacgcgaa ttcatgaaat ggatggccgc ctcgaccggc gcggcggcgg caggctgtgc
     2461 cggcacgggc ggcggatccg cgggccgcgt ggtggtggtc ggaggcggct acggcggcgc
     2521 gaccgccgcg cgctacgtca aggagtgggc ccccgatatc gaggtcacgg tcatcgagcg
     2581 caacaacatc ttcgtttcgt gcccgatcag caacctggtg ctgggcggca acacccagat
     2641 cggcaacatc acgatggggt acgacgggct gaggaagcgc ggcgtgcgcg tcgttcgcgg
     2701 ggaggcggtg gcggtggacg cggccgcgaa acaggtgcgg cttgcggatg gcaccacgct
     2761 cggctacgac cgggtcattg tttcccccgg catcgatttc atctacgact cgatcccggg
     2821 cctgaaaggc gccgacgcgc agaaccgcgt gctgcacgcg tggaaggcgg ggccgcagac
     2881 ggtggcgctg aggaagcagc tcgaggcgat gcgcgacggc ggcgtgttcg tgttccacat
     2941 cccgatggcg ccgtaccgct gtccgccggg accgtacgag cgcgtgtgcc aggtcgcgga
     3001 ttacttcaag agggcgaaac cccggtccaa gatcatcgtg ctcgactcga attcggacat
     3061 cacgtcgaag aagggcctgt tcctgcagtc ctggaacggc gtgtacaagg gcatgatcga
     3121 ttaccgcccc aacagcgagc tcgccgacgt ggacgtcaag ggcatgacgg tgaagctcac
     3181 cttcgatagc gtcaagggcg acgtgctgaa cgtcgtgccg ccgcatcgcg cgggcgacat
     3241 cgcgcgcacg acgggcctca tcaccgccaa ccagcgctgg tgcggcgtcg attggatgac
     3301 catggagtcc accgcggtca agggcgtgca cgtcctgggc gatgcgacgc tctccgcgcc
     3361 ggcgatgccc aagtcggcga gcatggcgaa ccagcacgcc aaggtctgcg ccgccgcggt
     3421 tgccgcgctg atcaagggcc agccggtcaa cccgctgccg gtgatgatga atacctgcta
     3481 cagcttcgtg agcggaaatg ccgccatgca cgtggcgtcg gtccaccagt acgactccgc
     3541 gcagaagacg atcgtccccg tgaagggcgc gggcggggta tcgccgtcgg caagcgagat
     3601 cgagggcgcc tatgcatggg gctgggcgcg caacatctgg gcggacgcgc tcggctgatc
     3661 gccgccttgc ctggagaaat cagcggcgct gcggcgccgt tgtttttttt gccgggcgca
     3721 cccaccacca cgttgccgcg accagcagcg cgaagccgac gtgcaggagg gtgaagaccc
     3781 attccggcca gtcgtaatac aggagccggt gaacccactg cgcgatgaag cccttttccg
     3841 cctgcgttcc gcgcagcgcg gcttcccaca cggtgagcgg gcaccacatt ccgatcaatg
     3901 cctccagggc gacgaaaccg atcgcggcga ggtgcgcggc gcggaaccga agattgcgca
     3961 cccagtccca gccggccgcc gcgccgatcc agatgagcgc cagcccgccc accacgaaca
     4021 gcacgaacgc gaagtgaacg acgagcacga catcggcgag ccactggctc atgggcgggt
     4081 cgggcacacc gcgatggaac tcatccgcgg cccgcgggac ttagagtccc taacataatg
     4141 aaacacgcgt gcgttgcgag ccaaaatggg tgatgacgcg aaggacggcg caggccctat
     4201 gtcgataggg ccaagccggc cgacaaagtg tcccgcgcag ggcgg
//
LOCUS       DBNGSG010000170         3614 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_906191, whole genome shotgun sequence.
ACCESSION   DBNGSG010000170 DBNGSG010000000
VERSION     DBNGSG010000170.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_906191"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="AC158Q_04430"
     CDS             complement(<1..608)
                     /locus_tag="AC158Q_04430"
                     /inference="COORDINATES: protein motif:HMM:NF014766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR3339050.1"
                     /translation="MKITKVETLVINMPMLIEGSVMPKQGGRARTSMDTLLVRVDTDE
                     GVTGWGEGFGHRIFSATKAALDSFIGPMCVGRDPTAISALFGELERNLAGVGRNGPAM
                     YALSAIDIALWDIAGKLARQPLYRLLGGSPRTKLPAYASLLRYGEPAAVTHYVECTLK
                     RGYRHIKLHEITFEPIAAARKAAGPGIEIMVDCNCPWTVAEA"
     gene            684..1808
                     /locus_tag="AC158Q_04435"
     CDS             684..1808
                     /locus_tag="AC158Q_04435"
                     /inference="COORDINATES: protein motif:HMM:NF024771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR3339051.1"
                     /translation="MKIAKITAHTLRVPFHFPLIKETQNALITFVEVETDDGLKGHAF
                     SGYPLRWSIAEFINREAADCVKGMDAMRPEAVRTALYVKLSNKLYMGVWSCAASLIDI
                     ALWDIKGKAVKQPIWKLLGGAREKCPIYITFGLPRYSRAELVEVAKQLIAEGHTQLKV
                     AIASGANPAAHMYGEPTDDDILEDAARIHHLRDAIGDKPVLMMDANKNAKLTQAIRLA
                     KLVEPCNMAWFEDPVLQADSRLMAQVRRETTIPIAAGSTGTSDISHLREYFINESIDI
                     AQPNVRDIGGFTGGLRAAGLAQAFNIQLEMGGNYPHLNMHLHAGVPNGGRVEFHLGGW
                     RIGEALFDGVPPPVKGWVTLPSAPGLGFTPKAGILDLAIK"
     gene            complement(1897..>3614)
                     /locus_tag="AC158Q_04440"
     CDS             complement(1897..>3614)
                     /locus_tag="AC158Q_04440"
                     /inference="COORDINATES: protein motif:HMM:NF003714.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HIR3339052.1"
                     /translation="YLNEIILLGSLLALLSIVASAASSRLGAPLLLVFLVLGMLAGED
                     GPGGIRFNDFYTAQLVGSIALAIIIFDGGLRTRKEVFHVALRPAVSLATVGVILTAAM
                     VGVFATWLLDLHWMQGLLLGAIIGSTDAAAVFGLLQTAGTQLKQRVAATLGIESASND
                     PMAIFLTVVLVGVLAAGGTSLSPGILVNFVQQFGIGAVVGWFGGRLLGALINRLNLVT
                     ALYPLLAATGGIFIFGVAAVLNGSGFLAIYLAGIVLGNMPLQASQNILRMHDGLAWLS
                     QITMFLMLGLLITPHELFEYSYGGAVIALALIFVARPLAVLVSLLPFRFSWREHLFIS
                     WVGLRGAVPMILAIFPVTAGIPDAALYFNIAFFVVLSSLVIQGWTIAPAARLLSQEIP
                     PTTEPVQRINLDIPGHFDREIACYRVMPGCLAANRPIGEISISRDSHITTVVRDNVVM
                     TVSDGLKLLPDDLVYIFTDPAHVPELNRLFDPRQAPARLEERRFYGVFVLDGSASAGD
                     VADVYGLPIPEARRNETLDGYLGAVFHGRPVVGDRVRLGMAELVVRQIDDGRITRVGL
                     RIRQG"
BASE COUNT          685 a         1181 c         1205 g          543 t
ORIGIN      
        1 aacgcctccg ccaccgtcca cgggcagttg cagtcgacca tgatctcgat gccgggtccg
       61 gcggccttgc gcgcggcggc gatcggctcg aaggtgatct cgtgcagctt gatgtggcga
      121 tagccgcgct tgagcgtgca ctcgacgtag tgcgtgacgg cggcgggctc gccgtagcgc
      181 agcaggctcg cgtaggcggg cagtttcgtc cgcggcgagc cgccgagcag ccggtagagc
      241 ggctgccttg cgagcttgcc ggcgatgtcc cacagcgcga tgtcgatcgc ggacagcgcg
      301 tacatcgcgg gaccgttgcg cccgacgccg gcgagattgc gctccagctc gccgaacagg
      361 gcgctgatgg ccgtgggatc gcgcccgacg cacatcgggc cgatgaacga atcgagcgcc
      421 gccttcgtgg cgctgaagat gcggtgtccg aagccctcgc cccagccggt gaccccttca
      481 tccgtatcca cgcgcaccag cagggtgtcc atgctggtgc gggcgcggcc gccctgcttc
      541 ggcatcaccg atccctcgat cagcatcggc atgttgatga cgagggtttc gacttttgta
      601 atcttcatcg ctatagtgta tcgcccggtt gcggcgttcg cctcaagtcc gcagtgtggt
      661 cacccgacac atagaggatc ttcatgaaaa tcgccaagat cacggcgcac accctgcgcg
      721 tgccgtttca tttcccgctg atcaaggaaa cccagaacgc gctgataacg ttcgtcgagg
      781 tcgagaccga cgacggcctg aagggccacg cgttctcggg ctacccgctg cgctggagca
      841 tcgccgagtt catcaaccgc gaggcggccg actgcgtgaa gggcatggac gccatgcggc
      901 cggaggcggt gcgcaccgcg ctgtacgtca agctctccaa caagctctac atgggcgtgt
      961 ggtcgtgcgc ggcgagcctg atcgacatcg cgctgtggga catcaagggc aaggcggtga
     1021 agcagcccat ctggaagctg ctcggcggtg cgcgcgagaa gtgcccgatc tacatcacct
     1081 tcgggctgcc gcgctacagc cgcgcggagc tggtcgaggt ggcgaagcag ctgatcgccg
     1141 aagggcacac gcagctcaag gtcgcgatcg cctccggcgc caacccggcc gcgcacatgt
     1201 acggcgagcc gaccgacgac gatatcctgg aagacgccgc gcgcatccac cacctgcgcg
     1261 acgccatcgg cgacaagccg gtgctgatga tggacgccaa caagaacgcg aagctgaccc
     1321 aggcgatacg cctcgcgaag ctggtcgagc cgtgcaacat ggcgtggttc gaggacccgg
     1381 tgctgcaggc cgattcgcgg ttgatggcgc aggtacgccg ggagaccacc attcccatcg
     1441 ccgccggcag caccggcacc tccgacatct cgcacttgcg cgaatacttc atcaacgaat
     1501 cgatcgacat cgcgcagccc aacgtgcgcg acatcggcgg attcaccggt ggcctgcgcg
     1561 cggcggggct ggcgcaggcg ttcaacatcc agctcgagat gggcggcaat tacccgcacc
     1621 tcaacatgca ccttcacgcc ggcgtgccca acggcgggcg cgtcgagttt cacctcggcg
     1681 gctggcgcat cggcgaagcg ctgttcgacg gcgttccgcc gccggtcaag ggctgggtca
     1741 cactgccgtc cgcgccggga ctcggcttca cacccaaggc gggcattctc gacctggcga
     1801 tcaagtagac tggaaagcgc gtgacgggtg acgagtgacg ggtgatagga gccggctttc
     1861 gcgcgagcgc gaaacgcttc ccttatcact aaggactcag ccctgccgga tccgcaagcc
     1921 cacccgcgtg atgcggccgt cgtcgatctg ccgcacgacc agctcggcca tgcccagccg
     1981 cacccggtcg cctaccaccg gccgcccgtg gaagaccgcg ccgaggtaac cgtccagcgt
     2041 ttcgttcctg cgcgcttcgg ggattggcag gccgtagaca tccgcgacgt cgcccgcgct
     2101 cgcggagcca tcgagcacga agacaccata aaaccggcgc tcctccagcc gcgcgggcgc
     2161 ctggcgcggg tcgaacagcc ggttcagttc cggcacgtgg gcgggatcag tgaagatgta
     2221 gacgagatcg tcgggcagca gcttcagccc gtcgctgacg gtcatgacca cgttgtcacg
     2281 aaccacggtg gtgatatggc tgtcccggga aatggagatc tcgccgatag ggcgattggc
     2341 cgccaggcag cccggcatca cgcggtagca ggcgatttcg cggtcgaaat gcccgggaat
     2401 gtcgaggttg atgcgctgaa ccggctcggt agtgggcgga atctcctgcg agagcaggcg
     2461 cgcggccggc gcgatggtcc agccctggat cacgagcgag ctcaggacga cgaaaaacgc
     2521 gatgttgaag tagagcgccg cgtccggaat gccggcggtc acgggaaata tggcgagtat
     2581 catcggcact gctccccgca ggcccaccca gctgatgaac agatgctccc gccaggaaaa
     2641 cctgaacggc agcaggctga cgagcaccgc gagcggccgc gcaacaaaga tcagggcgag
     2701 cgcgatgacg gccccgccgt atgagtactc gaagagctcg tggggcgtga tgagcagacc
     2761 cagcatcagg aacatcgtga tctgggagag ccacgccagc ccgtcgtgca tgcgaaggat
     2821 gttctgggag gcctgcaacg gcatgttgcc gagcacgatg ccggcgaggt agatcgccag
     2881 gaacccgctg ccattcagga ccgcggccac gccgaatatg aagatgcccc cggtcgcggc
     2941 cagcagcgga tagagcgcgg tcaccaggtt cagccggttg atgagcgcgc cgagcagacg
     3001 cccgccgaac catcccacca cggcgccgat gccgaactgc tggacaaaat tcaccaggat
     3061 gccgggcgac aggctggttc cgccggcagc cagcacgccg accagcacca cggtcaggaa
     3121 gatggccatg ggatcgttgc tcgcggattc gattccgagc gtggcggcga cccgttgctt
     3181 gagctgggtg cccgcggtct gcagcaaacc gaataccgcg gccgcatcgg tcgagccgat
     3241 gatcgcgccg agcaacagcc cctgcatcca gtgcaggtcg agcagccagg tggcgaagac
     3301 tcctaccatg gcggcggtca gaatgacgcc gacggtcgcg agcgagacgg ccggtcgcag
     3361 cgctacgtgg aacacctctt tgcgggtgcg caagccgccg tcgaagataa tgatggccag
     3421 cgcgatgctg ccgaccaact gcgcggtata gaagtcgttg aaccggattc cgcccgggcc
     3481 gtcttccccg gccagcatcc cgagcacgag gaagacgagc agcagcggcg cgcccaggcg
     3541 cgaggacgcc gcgctcgcga caatgctcaa gagcgcgagc agcgagccca ggagaatgat
     3601 ttcgttcaaa tacg
//
LOCUS       DBNGSG010000171         6001 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3283772, whole genome shotgun sequence.
ACCESSION   DBNGSG010000171 DBNGSG010000000
VERSION     DBNGSG010000171.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6001)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3283772"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /gene="lptM"
                     /locus_tag="AC158Q_04445"
                     /gene_synonym="yifL"
     CDS             <1..197
                     /gene="lptM"
                     /locus_tag="AC158Q_04445"
                     /gene_synonym="yifL"
                     /inference="COORDINATES: protein motif:HMM:NF047847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPS translocon maturation chaperone LptM"
                     /protein_id="HIR3339053.1"
                     /translation="TAYRLPFAATILPMRALLPMIVCALLVAGCGYKGPLYLPKPKSE
                     ASKPTPKPAPEQDQKKSEGP"
     gene            194..1435
                     /gene="lysA"
                     /locus_tag="AC158Q_04450"
     CDS             194..1435
                     /gene="lysA"
                     /locus_tag="AC158Q_04450"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HIR3339054.1"
                     /translation="MSHFSYIANELHAEKVPLSRIAERYGTPCYVYSCAALTEAWRAF
                     DAAFAGRDHLVCYAVKANSTLAVLNLLARLGSGFDIVSGGELARVLAAGGDPRKVVFS
                     GVGKTAEEMRQALTAGIHCFNVESESELARLETVAAQCGTTAAVSLRVNPDVDPRTHP
                     YIATGLKENKFGVAYQDAPGLYRKARTMRHLAVIGIGCHIGSQLTDVAPFVAALDRML
                     ELADRLIAEGTPIAHIDIGGGLGIRYKDESPPPVADYARALTARLGNRALKLLLEPGR
                     ALTGHAGLLLTRVEYLKHGREKNFAVVDAAMNDLMRPALYDAWHEVLPVAAGSGGKTY
                     DVVGPVCESGDFLARARTLALREGDLLAIMAAGAYGMSMSSNYNTRPRAAEVMIDGAQ
                     AHVIRERESISELMAREMLLP"
     gene            complement(2173..2925)
                     /locus_tag="AC158Q_04455"
     CDS             complement(2173..2925)
                     /locus_tag="AC158Q_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR3339055.1"
                     /translation="MKLPVSEAELHAYVDGALTSARSAEVEAYLSEHPEDAARIAAYR
                     EQVAALHREYDPALNEPLPRRFEVIGRGRIRPMLRYAGVAAVFVLGTVVGWQLHAYNT
                     DRQAETTSWARRAAIAHVVYSPEVRHPVEVGADQEAHLVAWLSKRLGSPLKVPHLGGL
                     GYALVGGRLLPGDRGPVAQFMYQDGRGQRLTLYVRVNPDDSRETAFRFAQERGVGVFY
                     WLDRKLGYALSGEVDKPELLRVATAVHRLLNP"
     gene            complement(2922..3428)
                     /locus_tag="AC158Q_04460"
     CDS             complement(2922..3428)
                     /locus_tag="AC158Q_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3339056.1"
                     /translation="MSDRESLITQHILRLRRYARALTGDRAAADDLVQDTLERALSRF
                     HLWRQGSDLRAWLFTIMHNIYVNQARSRIRHPHETLDEPAADALHAREPDWGELRDID
                     DALSRLPGEQRAVLLLVGLEQFTYEEAARVLDIPLGTVMSRLSRGRERLRLILAGDPR
                     ASALKVVK"
     gene            complement(3631..5832)
                     /locus_tag="AC158Q_04465"
     CDS             complement(3631..5832)
                     /locus_tag="AC158Q_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-linked N-acetylglucosamine transferase family
                     protein"
                     /protein_id="HIR3339057.1"
                     /translation="MDQLATARQLFLDALALHEQGDLERAECLYKEALALAPERPSVM
                     NNLAAVYVASGKLAEAMALCRKLLQVNPRDEVALLNQGSCQLKSGSAGEALASYDGAL
                     QIRPDYADALSGRGGALLGLGRLEEALASCERALAVEPDHAETLNNRGHVLLKLRRPE
                     EALASFERALAKKPGYVEALYNRGANLLELNRPAEALESYDRALAGNPDHVEALNNRG
                     IALLELRRPEEALTSFDRALAIKPDYAEALGNRGLALLVLNRREEFIEGYKRLLDIKP
                     DYIYAPGHLLTAQMQGCEWSDYETSAARILEGIRAGKRADTPFSFLAISDSAADQLQC
                     SRMYVADKYPAAPRPVWRGERYRHDRIRIAYLSADFHAHATANLMAGLFEAHDRTRFE
                     TTAISFGPDIADEMRTRLRAAFDRFIDVRDRSGHETASLLRELEIDIAVDLKGYTAFC
                     RTEILSHRAAPIQVNYLGYPGTMGADYIDYILADRLVIPEQHHSFYTEKVVYLPDTYQ
                     ANDSRRQIAERAPTRAEAGLPEQGFVFCSFNNNYKITPKIFDVWMRLLQRTEGSVLWL
                     LEDNAAAARNLRREAEHRGVAPQRLVFAPRLRLDKHLARHRLADLFLDTLPCNAHTTA
                     SDALWAGLPVLTCLGTTFAGRVAASLLHAIGLNELVTHSLEEYEALALELAANRKRLD
                     DIRSRLAANRATHPLFDTDRFRRHIEAAYTKMWERHQRGEPPASFSVPAAS"
BASE COUNT         1080 a         1970 c         1858 g         1093 t
ORIGIN      
        1 tcactgctta ccgtttgccg tttgctgcta ccatcctgcc catgcgcgcc ctgctcccga
       61 tgatcgtctg cgccctgctg gttgccgggt gcgggtataa aggcccgctt tacctgccca
      121 agccgaaatc cgaggcgtcg aaacccaccc ccaagcccgc cccggagcag gaccagaaaa
      181 aatccgaggg gccgtgagcc atttcagcta tatcgccaac gagctgcacg ctgaaaaagt
      241 gccgctctcc cggatcgccg agcgctacgg cacgccgtgc tacgtttact cgtgcgccgc
      301 gctgacggaa gcctggcgcg ctttcgatgc ggcgtttgcc gggcgcgacc atctggtctg
      361 ctacgcggtg aaggccaatt ccacgctcgc ggtgctgaac ctgcttgccc gcctgggcag
      421 cggcttcgac atcgtgtccg gaggcgagct cgcgagggtg ctcgcggccg ggggcgaccc
      481 gcgcaaggtg gtgttctccg gcgtgggcaa gacggccgaa gagatgcggc aggcgctcac
      541 ggcgggcatc cactgcttca acgtcgaatc cgagagcgaa ctcgcgcgcc tggaaaccgt
      601 ggcggcgcag tgcggcacga ccgccgcggt gagcctgcgg gtcaatcccg acgtggaccc
      661 gcgcacccat ccctacatcg ccaccgggct gaaggagaac aagttcggcg ttgcctacca
      721 ggatgcgccc gggctgtacc gcaaagcgcg cacgatgcgc cacctcgccg tcatcgggat
      781 cggttgccat atcggctcgc agttgaccga cgtggcgccg ttcgtcgctg ctctcgaccg
      841 catgctcgag ctcgccgatc gcctgatcgc cgagggcacc cctattgcgc acatcgacat
      901 cggtggcggc ctcgggatcc gctacaagga tgaatctccg ccgcctgttg ccgactatgc
      961 gcgcgcgctg acggcgcgcc tgggcaaccg ggccctgaaa ctgctgctcg aacccggccg
     1021 ggcgctcacc ggccatgccg ggctgctgct gacccgtgtc gaatacctca agcacggccg
     1081 cgagaagaac ttcgccgtgg tggacgccgc gatgaacgat ctcatgcggc cggcgctcta
     1141 cgacgcctgg cacgaagtgc tgccggtcgc ggccgggagc ggcggcaaga cctacgatgt
     1201 cgtcggcccg gtatgcgaga gcggtgactt cctcgcccgc gcgcgcacgc tggcgttgcg
     1261 cgaaggggat ctgctcgcga tcatggcggc aggcgcttac ggcatgagca tgagctccaa
     1321 ctacaacacc cgcccgcgcg ccgcggaagt catgatcgac ggcgcgcaag cgcacgtcat
     1381 acgcgagcgc gaatccattt ccgagttgat ggcgcgcgag atgctgctgc cgtaaccgtt
     1441 cggcggaaat ttcaccgacg tttttgccgc cgccgtgcgc ccgctcgctt gttgcggcgg
     1501 atgcacatgc gacattccga tgtcaacccg aagcgataaa ggtattgcaa ttttttttca
     1561 ctgtggcata gaatgacttc accaggcata cgtttcccgg aaattgttcg gaaacacgcg
     1621 acgcgcagcg aagccagtcg cgcggacgat gcgatggtgg tgcggatcgc aagccgcacg
     1681 cagtcgattc agaagtcaac ctgaaaagga gatgtgtgat ggcgaagaaa aaggcaccga
     1741 agaagaaggc caagaagaag tagttggcgt gatgacggga gccgggcatg ttgtccggct
     1801 tccgtcattt gattcaccgt actgctgcag cagttttttc tccgcgccgc ttctcccctt
     1861 ttcgcttctt tacgttccgc acggctagtt tcgccgttcc catcgcttga tggcgagcgt
     1921 ataaaaacac caactaatca ataacatatg ctggataatg accggctgac gagcgtggca
     1981 atcatgcagt gcattgcgct cgcgacgtgt ctccgtgcta cagattggac gcaacgcaca
     2041 gtgaccgctg ctggataaaa gacgcggccg cgagttgaac ggcgcaggaa atcggttgca
     2101 gctactccag tactcccggg cgtccgttcg aactcgcgga gataaaccgg cgccggcagg
     2161 cgtccggatt tatcagggat tgagcaagcg atgcaccgcc gtcgccacgc gcagcaactc
     2221 gggcttgtcc acttcccctg acaacgcata accgagcttg cggtcaagcc agtagaacac
     2281 cccgacgccg cgctcctgcg cgaagcggaa ggcggtttcg cggctgtcgt ccggattgac
     2341 gcgcacgtag agcgtcagcc gctgtcctct gccgtcctgg tacatgaatt gggcgaccgg
     2401 cccgcgatca cccggcagca gcctgccgcc gaccagggca taccccaggc cgccaagatg
     2461 cggaaccttg agcggcgagc ccagccgctt ggacagccac gccacgagat gggcttcctg
     2521 gtctgctccg acctcaaccg ggtggcgcac ctccgggctg tatacgacgt gcgcaatcgc
     2581 cgcgcggcgc gcccacgaag tcgtctcggc ctgcctgtcc gtgttgtaag catgaagctg
     2641 ccagcccacg accgtaccca gcacgaatac cgcggcaacg cccgcgtagc gcaacatcgg
     2701 ccgaatcctt ccgcgaccaa tcacctcgaa ccggcgcggc agcggctcat tcagcgccgg
     2761 atcatattcc cggtgcaacg ccgcgacctg ttctcgatag gctgcgatac gcgccgcatc
     2821 ctccgggtgc tccgaaagat aagcttccac ttcggcggaa cgcgccgagg tgagcgcccc
     2881 atcgacatag gcgtgcaatt cggcctcgga gacaggcagt ttcatttcac cactttcaac
     2941 gctgacgcgc ggggatcccc ggcgagaatc aggcgcagcc gctcgcgccc gcgcgacagg
     3001 cgcgacatca ccgttccgag cggtatgtcg agcacccgcg cggcctcctc gtaggtgaac
     3061 tgttccaggc cgaccaggag cagcaccgcg cgctgctcgc ccgggagccg cgacagcgcg
     3121 tcatcgatgt cgcgcaactc gccccaatcg ggctcgcggg cgtgcagcgc atcggccgcg
     3181 ggctcgtcca gcgtttcgtg cggatgtctt atccgcgagc gggcctggtt gacgtaaatg
     3241 ttgtgcatga tggtaaacag ccacgcccgc agatcgctgc cctggcgcca cagatggaag
     3301 cgggaaagcg cgcgttccag cgtgtcttgc accaggtcat cggcggcggc gcgatcgccc
     3361 gtcaaggcgc gtgcgtaccg gcgcagccgc aggatatgct gcgtgatcag gctttcgcga
     3421 tcactcacga ccgcccccgt tcctgtaaac aatcgtggtt cccatcctat tccccaaccc
     3481 gcccggtcca tcctgaaccg cgggacgcga gtccaccggt ttccggagca ggcgcagcat
     3541 ccggataagt tgcccgtatg gtgccattaa tttgattgta tatcgggcat cctgtttctg
     3601 gagacgctgc agcggggctc ttgccgaatc tcatgatgca gccggcacgg aaaagctggc
     3661 cggaggctcg cccctctggt gccgctccca catcttcgta taggcggcct cgatgtggcg
     3721 gcggaaccgg tcggtgtcga acaagggatg ggtggcgcgg ttcgcggcca gtctcgacct
     3781 gatatcgtcg agacgcttgc ggttcgcggc cagctccagc gccagcgcct catactcctc
     3841 gagggagtgc gtgaccagct cgttcaatcc gatcgcgtgc agcaagcttg ccgccacgcg
     3901 gccggcgaac gtggtgccca ggcaggtcaa aacgggaagg ccggcccaca aggcgtcact
     3961 ggcggtcgta tgcgcgttac acggcagggt gtccagaaac aagtccgcca accgatgccg
     4021 cgcgaggtgc ttgtccagtc tcaggcgggg ggcaaacacc agccgttgcg gcgccacgcc
     4081 cctgtgttcc gcctcgcgcc gcaggttgcg cgctgccgcc gcattgtcct ccagcagcca
     4141 cagcacgctg ccctcggtgc gctgcaacag ccgcatccag acgtcgaata tttttggcgt
     4201 gatcttgtaa ttattgttga acgagcagaa cacgaatccc tgttcgggca gccctgcctc
     4261 ggcgcgcgtc ggggcccgct ccgcaatttg ccgccgcgag tcgttggcct ggtaggtatc
     4321 ggggaggtag acgactttct cagtatagaa agagtgatgt tgctcgggaa tgacgagccg
     4381 gtcggcgaga atatagtcga tgtaatccgc ccccatcgtc ccgggatatc cgaggtagtt
     4441 gacctggatg ggcgcggcgc gatgcgaaag aatctcggtg cggcagaaag cggtgtagcc
     4501 cttgaggtcc accgcgatgt cgatttccag ttcccgcagc agcgatgccg tctcgtggcc
     4561 gcttctgtcg cgcacgtcaa tgaagcggtc gaaggcggcg cgaaggcgcg ttctcatctc
     4621 atcagcgatg tcgggcccga acgatatcgc ggtcgtttcg aatcgcgtcc tgtcatgcgc
     4681 ttcgaacagg cccgccatca ggttcgcagt cgcatgagcg tggaaatcag ccgacagata
     4741 ggcgatccgg atcctgtcgt ggcggtatcg ctcacctcgc cacaccggcc ggggcgccgc
     4801 aggatacttg tcggcgacat acatccgcga acactgcaac tggtcagccg ccgaatccga
     4861 tatggcgaga aaactgaatg gagtatcggc ccgcttgcct gccctgatgc cctccaaaat
     4921 ccgtgccgcg ctcgtttcat aatccgacca ttcgcagccc tgcatctgtg cggtcagtaa
     4981 gtgaccgggc gcatagatgt aatctggctt gatgtccagc aagcgcttgt acccctcgat
     5041 gaattcctcc cgccgattga gcaccagcaa tgccagaccg cgattgccaa gcgcctcggc
     5101 atagtccggt ttgatggcca gcgcccggtc gaagctagtc agcgcctcct ccggccggcg
     5161 cagttccagc agggcgatac cgcggttgtt gagtgcctcg acatgatcgg gattcccggc
     5221 cagcgcgcga tcgtagcttt ccagcgcctc cgccggcctg ttgagttcca gcaggttggc
     5281 gccgcggttg tagagcgcct cgacgtaacc cggctttttt gcgagcgcgc gctcgaagct
     5341 cgcgagcgct tcctccggcc gcctgagctt cagcaggaca tggccgcgat tgttgagtgt
     5401 ttcggcatga tcgggttcca cggcaagcgc gcgctcgcaa ctcgccaacg cctcctccag
     5461 tcgcccgagc ccgagtaagg cgcccccgcg gccggagagt gcatccgcat aatcgggcct
     5521 gatttgaagg gccccgtcat acgaggcgag cgcctcccct gccgagcccg acttgagctg
     5581 gcagctgccc tgattgagaa gcgcgacttc gtcccgcgga ttgacttgca gaagcttgcg
     5641 gcacaacgcc atcgcctcgg caagttttcc agaggccacg taaacggccg ccaggttgtt
     5701 catgacgctc gggcgctccg gcgccagcgc cagcgcctct ttgtacaaac actccgcccg
     5761 ttccagatcg ccctgctcgt gcagcgccag tgcgtcgaga aataactgcc ttgccgtggc
     5821 caactgatcc atgtgcgcgg cttgccgcta gaccgcaaaa ctcgcgggcg gctcgcccct
     5881 ctggtgccgc tcccacatct tcgtataggc ggcctcgatg tggcggcgga acatttcggt
     5941 gtcgaacaag ggatgggtgg cgcggttcgc ggccagtctc gacctgatat cgtcgagacg
     6001 c
//
LOCUS       DBNGSG010000172         7355 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4642737, whole genome shotgun sequence.
ACCESSION   DBNGSG010000172 DBNGSG010000000
VERSION     DBNGSG010000172.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7355)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7355)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7355
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4642737"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="AC158Q_04470"
     CDS             complement(<1..226)
                     /locus_tag="AC158Q_04470"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3339058.1"
                     /translation="MVNPPKHSRLLILGSGPAGYTAAVYAARANLKPVLITGLAQGGQ
                     LMTTTDVDNWPADAMGVQGPELMERFLKHAE"
     gene            401..2155
                     /locus_tag="AC158Q_04475"
     CDS             401..2155
                     /locus_tag="AC158Q_04475"
                     /inference="COORDINATES: protein motif:HMM:NF015803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HIR3339059.1"
                     /translation="MGVTPKMAWTVLAVLAVAPSAFAQSSPLRHDTGGISPDSRLLSQ
                     AGPLKPAPAKSAVEPLAPAPTAPAEIVPVAPVASPKFDIRRYVLEGNTLLAQEKVEQV
                     LGPYTGQSKDFGDVQRALEALQAAYQAAGYGGVEVRLPEQELERGEVRFTVIEVKIGK
                     ITVEGNEHFSKENIRRSVPSLREGVTPNSRDIAKNMRLVNENPSKQSAVLLRGAERDG
                     EVDATIKVADIDPRRWSMSLDNTGNESTGRARLGIAYQHANLFDRDHILTTQFITSPT
                     KFKDVNVLGVGYKIPLYSRGDSIDFVAGYSNVDSGTVQNLFLVSGQGSIFGARYNQAL
                     PKWRELEHKLIYGLDYRAYQNQVTPEGSDDPLVPDITVHPLSLTYSASVRTTGQDLSF
                     YLNVAQNIPGGNDGKDEDFKKQGARFGVGTASYRLYRGGINYVRVVKNDWQFRGNFSA
                     QYTEDALVSPEQFGIGGADSVRGFNERYASNDKGLRTTLEIYSPDQAKSLGFGGGRIR
                     FLAFYDTGALYRNYAEPNEKESVHLDSGGVGMRLNYKNYFSARMDIAHVFHDGSGYNE
                     PDSRRNINKIHFSAAWVW"
     gene            2500..3210
                     /locus_tag="AC158Q_04480"
     CDS             2500..3210
                     /locus_tag="AC158Q_04480"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3339060.1"
                     /translation="MPAQFALNPQILKSVPLFSQFSDAQLSQVLSCVQHRSYPRNSFI
                     LRAGEETDALYIVLSGRVKVLIPDEEGHEVILSIIGPQEYFGEMGLLDDQSRSASVES
                     LESCEMLRLSKAGFTNILKDNFELAMLIIRNLVKRLREADRKIESLALIDVYGRVARL
                     LIDMAKFVDGKWVVEHAPAKQEIARMIGASREMVSRVVKDLHHKGLIRAEKRRIYVLD
                     KQSMQKRASTRHGDRRRS"
     gene            3446..4168
                     /locus_tag="AC158Q_04485"
     CDS             3446..4168
                     /locus_tag="AC158Q_04485"
                     /inference="COORDINATES: protein motif:HMM:NF016648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HIR3339061.1"
                     /translation="MSLHKLVLRQAGFLLLSLFSAAAMAAGAGTVTHLSGTLSVQRTD
                     GSVRILSQKSEVNPGDVLTTQRDSYAQINFTDGSSLTMRPNTQIKIEQYNFVQDRPQE
                     DNAFLRLVKGGLRTVTGLVGKRGNQDAYKIGTNTATIGIRGSSGDTIENSDGKCEGVT
                     PGCEKLPPGVYHTTYTGSYIMQNEGGSQIIGEGQFGFARDPKTPPVILPGDPGLNVSQ
                     LPFTLGVGGGRIQGPGQECVVR"
     gene            4358..6661
                     /locus_tag="AC158Q_04490"
     CDS             4358..6661
                     /locus_tag="AC158Q_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HIR3339062.1"
                     /translation="MLLSEKTSARTAKNPLPPKLAALVREFWWFALLGVALYLSLILY
                     TYNKADPGWSHSTTVAFFHNAGGRVGAWMADIMLYVFGLSAYWWVVFFSALVWWGFRR
                     IETVPRTDGDHRSYIVAAVGFIVVLAASSGIEALRFYSLKAALPQAPGGILGAIVGGS
                     LAQALGFTGATIILLVLFAAGLSLFTGVSWLTVLERLGTWSETAYLFLLNKWQERQDR
                     RVGEQAVIRRDEIVEVSKKKLEVHEPVRIEPPAADIPRSARVEREKQVPLFENLPDSL
                     LPPLSLLDEPEKSHETLSTETLEFTSRLIEKKLLDFGVEVKVVAAYPGPVITRYEIEP
                     AVGVKGSQVINLIKDLARALSVVSIRVIETIPGKSLMGLEIPNPTRQIVRLSEILGSQ
                     IYADVSSPLALALGKDIAGNAVVADLQRMPHLLIAGTTGSGKSVGINAMLLSLVYKSP
                     PSQVRLILIDPKMLELSVYEGIPHLLAPVVTDMRQAANALNWCTAEMDRRYKLMNALG
                     VRNMAGFNQKVRDAVKNKKPIPNPFAPLPESAEPLHEMSLIVVVIDELADLMMVVGKK
                     VEELVARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFQVSARVDSRTILD
                     QMGAEALLGQGDMLYLPAGSGFPQRVHGAFVADHEVHKVVDYLKKLARPQYVESVLEG
                     PESEEAGEGGLEGGDAESDPLYDQAVAIVLKTRRASISLVQRHLRIGYNRAARLIEQM
                     ERAGLVSAMQSNGNRDVLAPAGASEER"
     gene            6729..7220
                     /locus_tag="AC158Q_04495"
     CDS             6729..7220
                     /locus_tag="AC158Q_04495"
                     /inference="COORDINATES: protein motif:HMM:NF024901.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HIR3339063.1"
                     /translation="MKTLIGLMAFLSAGACLCAEVYKWVDEKGVVNYGEKPPARRPAQ
                     PVDTNPRTVIETDGRFGQMSGAERGRAGAAPQVIAAPASALTAFSPRGMEFDIYIRLQ
                     PGMTEGELLTRAGRPDHESLDSLVYDIVKTYYYFPTTANPYTTVVTLRGGRIAELDRI
                     KKF"
BASE COUNT         1498 a         2182 c         2260 g         1415 t
ORIGIN      
        1 gctcggcgtg cttgagaaac cgctccatca attcgggtcc ctgcacaccc atggcgtcgg
       61 cgggccagtt atccacatcg gtcgtggtca tcaactggcc gccctgcgcg agcccggtga
      121 tcagtactgg cttgagattt gcccgtgccg catagactgc tgcggtataa ccggccggcc
      181 ccgatccgag tatcagcagg cgggaatgtt tgggagggtt gaccatacaa cggacagata
      241 ggggttattc gcaaaaaagc aacactctaa cagcgcgtac ataaccatgt cgagttacgc
      301 cactacctca actgtctgga tttaagtctt tgctataaaa tacaaaattg ggtcggtgca
      361 cgcagcgttt ttctgccccg agccagaaca ggggaggccg atgggggtta cgccgaaaat
      421 ggcgtggact gtgcttgctg tacttgcggt agcgccatca gctttcgccc aatcctcgcc
      481 actgcgccac gacaccggcg gcatctcgcc ggattcccgc ctgctcagcc aagccgggcc
      541 gctcaagccg gcgcccgcta aatccgcagt ggagcccctc gcgcccgcac caacggctcc
      601 cgcagagata gtgccggttg ctcctgtcgc atctcccaag tttgacattc gccgttatgt
      661 cctggaaggc aatacactcc ttgctcaaga aaaagtcgaa caggtgctgg gcccctacac
      721 cggacagagc aaggattttg gggacgttca gcgggcgctg gaagcgttgc aggctgctta
      781 ccaggcagcc ggatatgggg gggtggaagt ccggcttccg gagcaggaac tcgagcgcgg
      841 cgaggtcagg ttcaccgtga tcgaagtcaa gatcgggaaa atcacggtcg aaggcaacga
      901 gcacttcagc aaggaaaaca ttcgccgcag cgttccatcg ttgcgcgaag gcgtcacgcc
      961 gaattcccgg gacattgcga aaaacatgag gctggtaaac gaaaatcctt cgaaacagtc
     1021 ggcggtactg ttgcggggcg cggagcgcga tggcgaggtt gacgcaacaa tcaaggttgc
     1081 cgacatcgat cccaggcgct ggagcatgag tctggacaac accggcaacg agagcaccgg
     1141 ccgcgcccgg ctggggattg cctatcagca tgcgaatctt ttcgatcgcg accatatcct
     1201 caccactcaa ttcatcacgt cgccgacgaa attcaaggat gtcaacgtcc tgggcgtggg
     1261 gtacaagatc ccgctgtatt cacgtggcga ctcgatagat ttcgtggcgg gctactcgaa
     1321 tgtggattcc ggcacggtgc agaatttgtt cctggtcagc ggccagggat cgattttcgg
     1381 cgcgcgatac aaccaggcgt tgcccaagtg gcgcgagctc gaacacaaac ttatttacgg
     1441 gctcgactat cgcgcttatc agaaccaggt cacccccgag gggtcggatg accctctggt
     1501 tccggacatc accgttcatc ccctgagcct gacctacagc gccagcgtgc ggaccacggg
     1561 gcaggatttg agtttttatc tgaacgtggc ccagaacatt ccgggcggga acgatggaaa
     1621 ggacgaagat ttcaagaagc agggcgcacg tttcggtgtg ggcactgcga gttaccggtt
     1681 gtatcggggc ggtatcaact atgtccgtgt cgtgaaaaac gactggcagt tcaggggcaa
     1741 cttcagtgcg cagtacaccg aagacgcact cgtctctccc gagcagttcg gaattggcgg
     1801 ggccgacagt gtcaggggat tcaacgagcg ctatgcctcg aacgacaagg gtctgcgaac
     1861 caccctggaa atctattcgc cggatcaggc caagtctctg ggattcgggg gcggccgtat
     1921 ccggtttctc gctttctacg acacgggcgc gctataccgg aattacgccg agcccaatga
     1981 aaaggaatcg gtgcacctgg atagcggggg agtgggcatg cgcctgaact acaagaacta
     2041 cttttcggcg cgaatggata ttgcccatgt tttccatgac ggaagcggct acaacgagcc
     2101 ggatagcaga cgcaatatca acaagatcca tttttccgcg gcctgggtat ggtgaaacgg
     2161 tcgacgactg caatggcagt caagagtcgc attttcagat taatcaaccg tctaatgcgc
     2221 gaccgccacg ggtatgcgcc tccgcaatgg ttatcgcggg agccggagcc ggggcgcaga
     2281 ttaccggtag cgggatcctg acgacatcga accgtaactt gctcaactgc cgcggcagcg
     2341 ccaactctcc ccggcgacaa ggcgattcca agggccggct ggcacctgtc gttagcgggg
     2401 gcgacgtgac gccagacgct tgatctggca gactatggcc tagctttcac ggtgccgacc
     2461 ggtgccgtga cgtagaacaa gaacccgggg cggaatgaca tgccggcaca gtttgcgctg
     2521 aatccgcaga ttctcaagag cgtaccgctg ttctcccagt tttccgacgc gcagctctcc
     2581 caggtgctga gctgcgtgca gcatcgtagc tacccccgca attccttcat tcttcgcgcc
     2641 ggcgaggaaa ccgacgcgct gtacatcgtc ctgtccggcc gggtgaaggt tctgattccc
     2701 gacgaggaag ggcacgaagt gattctttcc atcattgggc cacaggagta tttcggggag
     2761 atgggcctgc tcgacgacca gtcgcgttcg gccagcgtgg aatcattgga gtcgtgcgag
     2821 atgctgcggc tgtcgaaagc cggctttacc aacatcctca aggacaactt cgagctggcg
     2881 atgctgatta tccgcaacct ggtcaagcgc ctgcgcgaag ccgatcgcaa gatagaaagc
     2941 cttgccctga tcgacgtcta tggccgcgtg gcccgcttgc tgatcgacat ggcgaaattc
     3001 gtggatggca agtgggtggt ggagcatgcc ccggccaagc aggagatcgc gcggatgatc
     3061 ggtgcttcgc gtgaaatggt cagccgcgtg gtcaaggatc tgcatcacaa gggcttgatt
     3121 cgcgcggaga aacgccgaat ctacgtgctc gacaagcaat ccatgcagaa gcgggcctcg
     3181 acccgccacg gcgatcgccg ccgctcctga tcgaaagtcg acccctgacg tcaccgcagt
     3241 aaccgattgc gtcagccccg gttacttggc ctatttccta atcgattgat ttcaagaagt
     3301 tatatgccct gcacccgggg atacttgtga gcatttgttc actgttcgca acgtccgctt
     3361 gtggtataaa ttttgctgcg gatacaacga gtttccggag ggacccggtc catccggatg
     3421 gaaatgaaca gagggagtgg aaaccatgag cctacacaag ctggttctga gacaagctgg
     3481 attcctgttg ctgtcgttat tcagcgcggc agcaatggcg gcaggtgccg gcacggtgac
     3541 gcacctgagc gggacgctct cggtgcagcg gactgatggc agcgtacgga tcctgtcgca
     3601 gaagtcggag gtgaaccccg gcgacgtgct gacgacgcag cgcgacagct acgcgcagat
     3661 caatttcacc gacggcagct cgctcacgat gcgccccaac acccagatca agatcgagca
     3721 gtacaatttc gttcaggatc gtccccagga ggacaatgct ttcctgcgtc tggtcaaagg
     3781 cgggctgcgc acggtgacgg gccttgtcgg caagcgcggc aaccaggatg cctacaagat
     3841 cggcaccaac acggcgacga tcggaatccg aggctcctcg ggcgacacga tcgagaactc
     3901 cgacggcaag tgcgaagggg tcacgccggg ctgcgagaag cttccgcccg gtgtctatca
     3961 cacgacttac acgggcagct acatcatgca gaacgagggc ggttcgcaga taatcggcga
     4021 agggcagttc ggatttgccc gcgacccgaa gacgcccccc gtcatcctgc ctggcgatcc
     4081 tggcctcaat gtcagccagt tgccgtttac ccttggggtg ggcggcggac gtattcaggg
     4141 tcctggccag gagtgcgtgg tgcggtaagc acgcgtatcg ctacacaact gcaaaccccg
     4201 cctggtgcgg ggtttgtttt tgtacaacct catgcccgtg cttcgcgctt gacggcccac
     4261 cgccgtaatt gtcgactccg gcatgaacgg atgccacggt tcttactcca ggcgctttcg
     4321 atgtacaatc aaaaagttaa ccggagcact ccacttaatg ctgttgagcg aaaaaacatc
     4381 agcgagaacg gccaagaatc cgttgccgcc caagctggct gctctggtcc gggaattctg
     4441 gtggtttgcg cttctcgggg tcgcccttta cctctcgctc atcctctata cctacaacaa
     4501 ggctgatcca ggctggtccc atagcaccac tgtcgcgttc ttccacaatg ccggcggcag
     4561 ggtgggtgca tggatggccg acatcatgct ctacgtattt ggactctccg cgtactggtg
     4621 ggtggtgttt ttttccgcct tggtctggtg gggcttccgg cgaatcgaga ccgtgccgcg
     4681 cacggatggt gaccaccgct cttacatcgt cgcggcggtc gggttcatcg ttgtcctggc
     4741 ggcgagcagc ggcattgaag cactgcgctt ctacagtctc aaggcggcgc tgccgcaggc
     4801 gcctggtgga atcctcggtg cgatcgttgg cggatccctg gcccaggctt tgggctttac
     4861 cggcgctacc atcattctcc tggtgctgtt tgccgcgggc ctgagcctct ttaccggcgt
     4921 gtcctggctg accgtgctcg agcgcctggg aacctggtct gaaaccgcct acctgtttct
     4981 gttgaataag tggcaggagc ggcaggaccg gcgcgtgggc gaacaggcgg taatccgtcg
     5041 cgacgagatc gtcgaggtca gcaagaaaaa gctcgaagtg cacgaaccgg tccgcatcga
     5101 gccgcccgcg gccgacattc ccaggtcggc gcgcgtcgag cgcgaaaaac aggtcccgtt
     5161 gttcgagaac cttcccgatt cgctgctgcc cccattgagc ctgctcgacg agccggagaa
     5221 gagccacgag acattgagca ccgagaccct cgagttcacc tcgcgcctga tcgagaagaa
     5281 gctgctcgat ttcggagtcg aggtgaaggt ggtcgccgcc tatcccgggc cggtgataac
     5341 ccgctatgag atcgagcccg ccgtcggcgt caagggcagc caggtcatca atctgatcaa
     5401 ggatctcgcg cgggcgctgt cggtggtcag catacgcgta atcgagacca taccgggcaa
     5461 gagcctgatg ggactcgaga tccccaatcc gacgcgccag atcgtgcggc tgtcggaaat
     5521 tctgggttcc cagatctacg ccgacgtctc gtccccgctc gctttggcgc tggggaaaga
     5581 catcgccggc aacgccgtcg ttgccgacct gcagcgcatg ccgcacctgc tgatcgccgg
     5641 cacgaccggc tcgggcaagt ccgtgggaat caacgccatg ctgctgagcc tggtctacaa
     5701 gtctccgcca tcccaggtca ggctgatcct gatcgacccc aagatgctcg agttgtcggt
     5761 atacgaaggc attccgcatc tgctggcgcc ggtcgtcacc gacatgcgcc aggctgccaa
     5821 tgcgctcaac tggtgcactg ccgagatgga ccggcgttac aagctgatga atgccctcgg
     5881 cgttcgcaat atggccgggt tcaatcagaa ggttcgtgat gcggtcaaga acaagaagcc
     5941 gattccgaac ccgttcgcgc cgctgcccga gagcgcggag ccgctgcacg aaatgtcgtt
     6001 gatcgtcgtg gtcattgacg agctggccga cctcatgatg gtcgtcggca agaaggtcga
     6061 ggagcttgtt gccaggctgg cgcagaaggc gcgcgccgcc ggcatccacc tcgtgctggc
     6121 aacccagcgc ccgtcggttg acgtcatcac cggcctgatc aaggccaata ttcccacccg
     6181 catcgcgttc caggtatcgg cacgcgttga ctcgcgcacc atactcgacc agatgggagc
     6241 ggaagcgctg ctcggccagg gcgacatgct ctacctgccg gccggaagcg gctttccgca
     6301 gcgcgtgcac ggggctttcg tcgccgacca cgaggtgcac aaggtggtgg attacctcaa
     6361 gaaactggca cggccgcaat acgtggaaag cgtgctggaa ggacccgaat ccgaggaagc
     6421 cggcgagggc ggtctggaag ggggggacgc cgaatcggat ccactctacg accaggcggt
     6481 cgccatcgta ctgaaaaccc ggcgcgcgtc gatctcgctg gtgcagcgcc acctgagaat
     6541 cggctacaac cgcgctgccc ggctgatcga gcagatggag cgtgccggcc tcgtgtccgc
     6601 gatgcagtcg aacggcaatc gggatgtgct ggcgccggcc ggggcgtccg aggagcggta
     6661 gttttcgtat agaatttccg acgccggtcg ctgtctcacc ggacacgact atggcggaca
     6721 atctatccat gaaaacgctg attggcctga tggctttcct gtcggccggg gcgtgtctct
     6781 gcgccgaagt ctacaagtgg gtggatgaga agggagtcgt caactacggt gaaaagcctc
     6841 ccgcccgccg gccggcacag ccggtggaca cgaatccccg aacggtgatc gagactgacg
     6901 ggcgattcgg ccagatgagc ggggctgaga gaggacgcgc cggggcagcc ccgcaagtca
     6961 ttgccgcgcc ggcgtccgcg ctaactgcct tttcgccccg cggcatggaa ttcgatatct
     7021 acatccgcct gcagcccggg atgactgagg gcgaattgct gacgcgtgcc ggccggcccg
     7081 atcacgaaag cctggatagt ctcgtgtacg acatcgtgaa gacctactac tactttccga
     7141 ccacggccaa tccctacacc accgtggtga ccttgcgcgg cggccgcatt gccgagctcg
     7201 atcgcatcaa gaaattctga tgcccaagat actcctgctg ctggccgttc tgctgccgga
     7261 aatcgcgggc gcggcgagcg tcgaaaatct caagggcctg ctcgagcaga ccacgacggc
     7321 gcgcgcgcgt ttcgcgcaaa tggtgctcga ccgga
//
LOCUS       DBNGSG010000173         2491 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2095385, whole genome shotgun sequence.
ACCESSION   DBNGSG010000173 DBNGSG010000000
VERSION     DBNGSG010000173.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2095385"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            236..1204
                     /locus_tag="AC158Q_04500"
     CDS             236..1204
                     /locus_tag="AC158Q_04500"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3339064.1"
                     /translation="MRTVTRLMVYAACAALSPLAAAQPYPSKPIRMIAPSSPGGPVDT
                     IARVVAQGMVETLGQQIVIENRAGAAGQIGADLVAKSAPDGYTVLFGFSGPLAISPNV
                     SENTPYDSLKDFAAVTQVAAAPYVLLVHPTVPAKSVKQLIALARERPGKMNFASGGNG
                     TGLHMAGELFNVTAGIKIVHVPYKGANPGVAALMGGEVDMMFNGLSAALPHMRANRVR
                     PLAVGGDKRSPLVPELPTVAESGLKFNTTGWYGIVAPRNTPQAIVAKLHAETARALNL
                     PAVRDQLTKLAVDGIGSTPEQFTTLIREELAMWAKVVKTAGLKGKR"
     gene            1246..1944
                     /locus_tag="AC158Q_04505"
     CDS             1246..1944
                     /locus_tag="AC158Q_04505"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR3339065.1"
                     /translation="MKTRTLQVPLRDGVMGAHVAYPDRAPAGAVIAIMEIWGVNDTMR
                     RHAAEFAEAGFICLVPDLFWRQEPGVELYDSKPEDVKKAFDLYYDFDYDLGVRDMEDT
                     WKVLAGVPECNGKVGAVGYCLGGKLCYLLCCRTDIDCAVAYYGTYIEHRIREAPNLHR
                     PFMLHMAMKDRWVQAEVNELLERRLSPNPLVTIHQYPGADHAFARHGGKTYSKPEADR
                     ALALTVEFFRKHIA"
     gene            2060..>2491
                     /locus_tag="AC158Q_04510"
     CDS             2060..>2491
                     /locus_tag="AC158Q_04510"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3339066.1"
                     /translation="MALPLTIDTHAHYFPESYLKLIADHGNRCGTTIVADASGARFIQ
                     VGLALRTGPITPHFYDLDDRIRTMDAIGVRMHALSLTQPMVYWADDELGLQLCVAFND
                     AVSEAHRRHPERFIGFACLPLQNANLALEELERVSRLPGIRG"
BASE COUNT          449 a          813 c          848 g          381 t
ORIGIN      
        1 caaggagata ttcgccgcca acgccgccga ggcgttgagg atgagccccg acgagctgca
       61 gaaggcgctg gagcgggacg tgaaggcgtg ggccgaggtc gtcaaggcca cgggcgttag
      121 aatcgactga ggggcctcgc gcgcggccga aacatggaac aatggacgct gcgcccggcg
      181 ccgccgacgg cggttgccgg agggcccata acatagcgac tatcgaggag gaatcatgag
      241 aacggttacc aggttgatgg tgtatgccgc gtgcgccgca ttgagcccgc tggcggcggc
      301 gcaaccgtat cccagcaagc cgatccggat gatcgcgccg tcctcgcccg gcggcccggt
      361 ggataccatc gcgcgcgtcg tggcgcaggg catggtcgaa acgctgggcc agcagatcgt
      421 gatcgagaac cgcgccggag cggcgggaca gatcggggcc gacctggtcg cgaagagcgc
      481 gcccgacggc tatacggtgc tgttcggttt ctcggggccg ctcgcgatct cgcccaatgt
      541 gagcgaaaac acgccgtatg attcactgaa ggattttgcc gcggtcacgc aggtcgccgc
      601 ggcgccgtac gtgctgctgg tgcatcccac cgtgccggcg aagtcggtga agcagctgat
      661 cgcgctcgcg cgggagcgcc cggggaagat gaacttcgcc tccggcggca acggcaccgg
      721 gctgcacatg gcgggggaac tgttcaacgt caccgcgggc atcaaaatcg tccacgtgcc
      781 ctacaagggc gccaatccgg gcgtcgccgc gctgatggga ggcgaggtcg acatgatgtt
      841 caacgggctg tcggcggcgc ttccccacat gcgggcaaac cgggtgcgcc ccctggcggt
      901 aggcggagac aagcgctcgc cgctggtgcc ggaactgccg accgtcgcgg aaagcggcct
      961 caagttcaac accaccggct ggtacgggat cgtcgcgccc cgcaacacgc cgcaggcgat
     1021 cgttgcgaag ctgcacgccg agaccgcgcg cgcgctcaac ctgcccgcgg tcagggacca
     1081 gttgaccaag ctcgcggtcg acggcatcgg ctccacgccc gagcagttca cgacgctcat
     1141 acgcgaggaa ctggccatgt gggccaaggt ggtcaagacc gcgggcctga agggcaagcg
     1201 ctgaccgggg cgcggccact tttcgcgaac ctgcacgcgc ggcgcgtgaa aaccaggacc
     1261 ctccaggttc ccctgcgcga cggcgtgatg ggggcgcacg tcgcgtaccc ggaccgcgcg
     1321 ccggccggcg cggtgatcgc gatcatggag atctggggcg tcaacgacac catgcgccgc
     1381 cacgccgccg aattcgccga ggcgggcttc atctgcctgg ttccggatct cttctggcgg
     1441 caggagcccg gtgtcgagct ctacgactcg aagccggagg acgtgaaaaa ggccttcgac
     1501 ctgtattacg acttcgatta cgacctcggc gtgcgcgaca tggaggacac gtggaaggtc
     1561 ctggcgggcg tgcccgagtg caacgggaag gtcggcgcgg tgggctactg cctgggcggc
     1621 aagctttgtt atctcctgtg ctgccgcacc gacatcgatt gcgcggtcgc ctactacggc
     1681 acctacatcg agcaccgcat ccgcgaggcg cccaatctgc accgcccctt catgctgcac
     1741 atggcgatga aggaccgctg ggtgcaggcg gaggtgaacg agttgctgga gcgcaggctc
     1801 tcgcccaacc cgctggtgac gatccaccag tacccgggcg cggaccacgc gttcgcgcgg
     1861 cacggcggaa aaacctacag caagcccgag gcggaccggg cgcttgcgct gacggtcgag
     1921 ttcttccgga agcacattgc ttgaaaagcg tgagtcgtga ttcgtgagtc gtgattcgac
     1981 ccgatacaga acgccggtct gaagctgcgc acgaatcaca aatcgcgatt cacgaatcac
     2041 ggcttataag gagcaactcg tggcgctgcc gctgacgatc gatacccacg cgcactactt
     2101 tcccgagtcg tacctgaagc tgattgccga ccacggcaat cgctgcggca cgacgatcgt
     2161 cgccgacgcc tcgggcgcgc gcttcatcca ggtcggcctt gcgcttcgca ccgggccgat
     2221 cacgccgcat ttttacgatc tcgacgaccg catccggacc atggacgcga tcggcgtgcg
     2281 catgcacgcg ctgtcgctga cgcagccgat ggtctactgg gcggacgacg aactgggcct
     2341 gcagctgtgc gtcgccttca acgacgcggt gagcgaagcg caccgcaggc acccggagcg
     2401 cttcatcggc tttgcctgcc tgccgctgca gaacgccaac ctcgcgctgg aagagctcga
     2461 gcgcgtgtcg cgcctgcccg gcatccgcgg c
//
LOCUS       DBNGSG010000174         4133 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_907887, whole genome shotgun sequence.
ACCESSION   DBNGSG010000174 DBNGSG010000000
VERSION     DBNGSG010000174.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_907887"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..554
                     /locus_tag="AC158Q_04515"
     CDS             168..554
                     /locus_tag="AC158Q_04515"
                     /inference="COORDINATES: protein motif:HMM:NF013970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HIR3339067.1"
                     /translation="MLKTPFVIDASAIAAVLFDEPEAAPIMASVSGSLQAPGLLRYEM
                     ASVCSIKLQREPKSAKLILLRYRLLSGLDIEFTEPDWDTLPVLARQWNLSAYDAAYLQ
                     LALQLKAPLVTLDTRLAAAYDKASKQ"
     gene            893..1879
                     /locus_tag="AC158Q_04520"
     CDS             893..1879
                     /locus_tag="AC158Q_04520"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3339068.1"
                     /translation="MNTRAKGIPAFVACTLCMCGLWTAAVCAQDRYPSRPIRLVIPFA
                     PGGGSDVTARMLGPRIAERLGQPVVIDNRPAASGVVGADIVAKAVPDGYTLLGTTVTF
                     VINGTLQKGLPYDAFKDFTPITQGIVSPFGLLLHPSVPAKTVKEFIAYGRSNPGKLLY
                     GSSGPGSSPHLSAELFNSMAGLKMTHVPYKGIAPAITAQLGNEVQMTFSNVFSTLGHW
                     KAGRLRLVAHGGLKRAESFPDIPTIAEAGLPGFESSNWYGYVTTAKTPRTIVRTLNQA
                     FVAVLNQPDVRKTLVAQGNDVIANTPEEFARVIQADAARWGGIGRKLGVKLN"
     gene            2054..3055
                     /locus_tag="AC158Q_04525"
     CDS             2054..3055
                     /locus_tag="AC158Q_04525"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3339069.1"
                     /translation="MQSLNRTLRAAALALAGACLAQPLAAAAADLAKDYPNKPVRFIV
                     PFAPGAGTDTTARTIAQKLSDAWGQQVVVDNRTGAAGAIGVDLTAKAVPDGYTICLIS
                     ASHSVNSAVNSKLPYDLTKDLAAISQASSLFYVVYLNPASVPVKSIKELVAHAKANPG
                     KLNFGSSGTGGLQHFAGEMFNYVAGTKMTHVPYKGTAAVIPAMLAGDVQVGFGTLFGV
                     RPHLPSGRLRALAVTTGKRTPAAPELPTVAEAGVPGYEVDQWYGVLTGAKVPAPIVRK
                     INAAIGEALKSPDVVKRFSADGSTPTSSTPEQFGKHIRSEIAKWRKLAKAAGLQLTQ"
     gene            3181..>4133
                     /locus_tag="AC158Q_04530"
     CDS             3181..>4133
                     /locus_tag="AC158Q_04530"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA dioxygenase family protein"
                     /protein_id="HIR3339070.1"
                     /translation="MEHLSQSSQVNPAKVAGAVNVNSLASFRVVPTGAPLGAEIRGVD
                     FSAPVGEDMKAALRKAWADHMVLLVRGQTLTDDQLLATSLIFGPPHDSAARKYHLAAG
                     HGVDDSYMVSRHPSITIISNLDASGNPVLDNGGLGSYEVVWHTDNSYVAVPPAGSMLY
                     ALEVPVNGGGDTSFNNQYLAYEELPDPLKRAIEGKYQVHDSSRNSAGVLRPGVKLPTK
                     PEEVEGPTHPLVRVHPVTGRRALYLGRRRVWPSNYIIGIPNEESERLLDELWAHATQP
                     KYAWTHKWRAGDLVLWDNRCCMHYRTEIDTAQRRVMHRTTI"
BASE COUNT          749 a         1406 c         1298 g          680 t
ORIGIN      
        1 tcgcatcccc ggaaaccggg gcccctgctc cacgttccgg cgcgttgaca attctggccg
       61 caaccccttg gggctggcgc ggttttggct cacgcctgct ccgcgctccc ttcatggtgc
      121 tattccctgt gtagctaaaa ccgtgacagc gcagcgcgtg tttcatcatg ttaaagaccc
      181 ctttcgtcat tgacgcgtcg gccatcgcgg cggtcctgtt cgatgaaccg gaagcggcgc
      241 ccatcatggc gagcgtaagc ggctctctgc aggcgccggg cctgctgcgc tacgagatgg
      301 caagcgtctg ctccatcaag ctccagcgcg agccgaaatc ggcgaagctc attcttctgc
      361 gctatcggct gctctcgggc ctggacatcg agttcaccga gcccgactgg gacacgcttc
      421 cggtgctggc ccgccagtgg aatctgagcg cgtacgacgc cgcctacctg caactcgctc
      481 tccagctcaa ggcgccgctg gtcactctcg acacccggct cgccgccgcc tacgataagg
      541 cctcaaagca gtgacgcgaa tcctggggtt cgcttgttta ttccgtcacg ccatcacttc
      601 gtcatcgccg ttaaagggaa gaccatgaac gtccgctcgc gcgcatcagc aagccgcgga
      661 agacgcgctg gcggctgacc gcgatgggtt tcgatgtcgc cggcggtacc cccgaagcgt
      721 tcgcggcgat ttttcgggac aacaacgcgc gcctcggcaa gatgatacgg gaggccggaa
      781 tcaaggccga ctgatggctg cgggcatcgc ccgccggcca cgttcaccgc agcgggtaaa
      841 cgcctgctga atcctttatc atgaacaccc ggaacggcaa caggaggaaa ccgtgaatac
      901 cagggcgaaa ggaatcccgg cgttcgttgc atgcacgttg tgtatgtgcg gattgtggac
      961 ggccgcggtt tgcgcgcagg accggtatcc ctcgcgcccg atacgcctgg tcatcccgtt
     1021 cgcgcccggc ggcggcagcg acgtcacggc gcgcatgctg ggcccgcgga tcgcggagcg
     1081 gctcgggcag ccggtcgtga tcgacaaccg cccggccgcc tccggcgtcg tcggcgcgga
     1141 catcgtggca aaggccgttc ccgacggcta cacgctgctc ggcaccacgg tcacgttcgt
     1201 catcaacggc accctgcaaa aggggctccc gtacgacgcg ttcaaggatt tcacgccgat
     1261 cacgcagggg atcgtgtcgc cgttcgggct gctgctgcac ccgtccgtgc cggcgaaaac
     1321 ggtcaaggag ttcatcgcgt acggccgctc caaccccggc aagctcctct acggctcctc
     1381 ggggccgggc agctcgccgc atctctcggc cgagctgttc aactcgatgg ccgggctgaa
     1441 gatgacgcac gtgccgtaca agggcattgc gcccgccatc accgcgcagc tcggcaacga
     1501 ggtccagatg acgttctcca acgtgttctc cacgctgggc cactggaagg ccgggcgcct
     1561 gcggctcgtc gcccacggcg ggttgaagcg cgcggagagc tttcccgata tccccaccat
     1621 cgccgaagcg ggcctgccgg gcttcgaatc gagcaactgg tacggctacg tgacgaccgc
     1681 gaagacgccg cgcacgatcg tgcggacgct gaaccaggcg ttcgtcgcgg tgctgaacca
     1741 gcccgacgtg cgcaaaaccc tcgttgcgca gggcaacgac gtcatcgcca acacgccgga
     1801 agaattcgcc cgggtgatcc aggctgatgc cgcgcggtgg ggcggaatcg ggcgcaagct
     1861 cggcgtcaag ctcaactaga agaggccatc gcgaaaaccg ttatggccac ggaaacacac
     1921 ggaaccacac ggaaattctt gaatatttca gggatattgg cggggtcggg ttcctgtccg
     1981 tgtcattccg cgtgattccg tggcaaattt ttcaggccgg ttcgcgaata caatttccac
     2041 tggagaaaac gtgatgcaat cactcaacag aactctccgg gcggccgcgc tagcccttgc
     2101 cggcgcgtgc ctcgcccagc cgctcgccgc cgccgccgcc gacctggcaa aggattaccc
     2161 gaacaagccg gtgcgcttca tcgtgccgtt cgccccgggc gccggcaccg acaccaccgc
     2221 gcgcaccatc gcccagaaac tctcggatgc atggggccag caggtggtcg tggacaaccg
     2281 caccggcgcg gcgggcgcga tcggcgtgga tctcacggcc aaggcggttc cggacggcta
     2341 cacgatctgc ctgatctcgg catcgcattc cgtcaactcc gcggtcaatt ccaaattgcc
     2401 gtacgacctc acgaaggatc tcgcggcgat ctcgcaggca agctcgctct tctacgtcgt
     2461 ctacctcaac ccggcgtcgg tcccggtgaa gtcgatcaag gaactggtcg cgcacgccaa
     2521 ggcgaacccc ggcaagctca acttcgggtc gtccggcacg ggcgggctgc agcatttcgc
     2581 gggcgaaatg ttcaattacg tggccggcac gaagatgacc cacgtgccgt acaagggcac
     2641 ggccgctgtc atccccgcca tgctggccgg cgacgtccag gtcggcttcg gcacgctctt
     2701 cggcgtgcgt ccccacctgc cgagcggccg cctgagggcg ctggcggtca ccacgggcaa
     2761 gcgcaccccg gccgcgccgg aactgccgac agtcgccgag gccggcgtgc ccggctacga
     2821 ggtggaccaa tggtacggcg tgctgaccgg cgccaaggtg ccggcgccca tcgtgcgcaa
     2881 gatcaacgcg gcgatcggcg aagcgctcaa gtccccggac gtggtgaagc ggttctcggc
     2941 cgacggctcg acgccgacca gctcgacgcc ggagcagttc ggcaagcaca tccgctccga
     3001 gatcgccaag tggcgcaagc tcgccaaggc ggcggggctg cagctcacgc aatgacttca
     3061 gttacaaggt acgagactca aggtacaagg tacggcgttc aacccctcag ctttcttcac
     3121 cttggaactt gggccttgta ccttgaacct tgaatcttgt acctgcgtca aggaggcatc
     3181 atggaacatt tgtcgcaaag cagccaggta aaccccgcaa aagtggccgg cgcggtgaac
     3241 gtgaacagcc tggcttcgtt ccgcgtggtg ccgacgggcg ccccgctcgg cgcggagatc
     3301 cggggcgtcg acttctcggc ccccgtgggc gaggacatga aggcggcgct gcgcaaggcg
     3361 tgggccgatc acatggtgct gctcgtccgc ggacagacgc tgaccgacga ccagctgctc
     3421 gcgacttcgc tgatcttcgg cccgccgcac gactcggcgg cgcgcaagta ccatctcgcc
     3481 gccgggcatg gcgtggacga ttcctatatg gtgtcgcggc accccagcat cacgatcatt
     3541 tccaacctcg atgccagcgg caatccggtg ctcgacaacg gcgggctcgg cagctacgag
     3601 gtggtctggc acaccgacaa ttcctacgtg gcggtcccgc ccgcgggcag catgctctac
     3661 gcgctggagg tgccggtgaa cggcggcggc gatacctcgt tcaacaacca gtaccttgcc
     3721 tacgaggagc tgccggaccc tctcaagcgc gcgatcgagg gcaagtacca ggtccacgac
     3781 tcgagccgca acagcgcggg cgtgctgcgc ccgggcgtga agcttcccac gaaacccgag
     3841 gaagtcgagg gtccgacgca tccgctcgtg cgcgtgcacc cggtaaccgg caggcgcgca
     3901 ctctacctcg gccgccggcg cgtgtggccg tccaattaca tcatcggcat cccgaacgag
     3961 gaaagcgagc gcctcctcga tgagctgtgg gcgcacgcga cgcagccgaa atacgcctgg
     4021 acccacaagt ggcgcgccgg cgacctcgtg ctgtgggaca accgttgctg catgcactac
     4081 cgcaccgaga tcgacacggc acagcggcgt gtcatgcacc gcaccacgat caa
//
LOCUS       DBNGSG010000175         4301 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_398220, whole genome shotgun sequence.
ACCESSION   DBNGSG010000175 DBNGSG010000000
VERSION     DBNGSG010000175.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4301)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_398220"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="AC158Q_04535"
     CDS             complement(<1..216)
                     /locus_tag="AC158Q_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339071.1"
                     /translation="MKRNTALALALVSAFALSTTATYAQSDEKQEAPKPELVSQSDEK
                     QEAPKPELISQSDEKQEAPKPEMNSKSD"
     gene            complement(414..1850)
                     /locus_tag="AC158Q_04540"
     CDS             complement(414..1850)
                     /locus_tag="AC158Q_04540"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3339072.1"
                     /translation="MSQDSFFRSTSFRLLAWYAAVFGASVAVLLVVVYWIALAAIDEQ
                     LSDSIERESQVLVELYRGRGLDSVARAIQLRVVDLRAPRRYYLLQNGSGERVAGNLPP
                     MDPTEGETVLPASYLYPNRSAKADNPADAYPVVAQGQRLDNGEFLLVGESRYRVVKAR
                     EAIVRAFSWGIAITVLLAGIGGAALRGGFLRRIEEINRTTRSIMDGDLSQRMRTRGSG
                     DEMDLLALNLNAMLGRIQALMESLKRVSDDIAHDLRTPLSRLRHRLEVARDNAGPDGD
                     PVIEQSISELDAILETFSALLRIAQIEAGARRAGFSDVNLSQIASAVSDAYVPVAEDR
                     TQQLEIIIDPVPSIQGDRELLTQMMANLIENPIRHCPPGVGITVHLRQEAGMAVLSVA
                     DTGPGIPEPEREKVFRRFYRLESSRTTPGSGLGLALVKAVADLHGASVELSDNRPGLR
                     VTVRFHAASAAAQKSHQQANRSSPSVVA"
     gene            complement(1837..2514)
                     /locus_tag="AC158Q_04545"
     CDS             complement(1837..2514)
                     /locus_tag="AC158Q_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3339073.1"
                     /translation="MKILLIEDDPHTSAYVANGLREHGHVVDLADNGRDGLLRGSGGG
                     YDVLVIDRMLPGLDGLAIVKTIRAAGTRSPVLLLTTLGGVNDRVDGLEAGADDYLTKP
                     FAFAELLARVNALARRPPMAEEATVLRVGDLEMNRLKRTVTRGGTQIELQPQEFRLLE
                     YLMRNAGQVVTRTMLLENVWEFHFDPRTSVVETHISRLRAKIDRDFDEELLHTVRGSG
                     YSLGVSR"
     gene            complement(2801..3919)
                     /locus_tag="AC158Q_04550"
     CDS             complement(2801..3919)
                     /locus_tag="AC158Q_04550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3339074.1"
                     /translation="MKRLLLVGGGHSHVEVVRRFGTAPPHGADVILVSPERYTSYSGM
                     LPGLIAGHYGYHDCHIDLEALCRAARVTFERAEVTGLELAHNRARFRDGGERAFDLLS
                     LDIGSTPDTASAPGAPQFAVSVKPVAVLLAAWEKMLAEARHSPQSIAIVGGGAGGVEL
                     AAAMHHRLQSNGAAASRLSVVTDAPAILQSHPPGVRRDFERLFAERGIALHCACRAVK
                     VEAGIVHFENGTSLRADWIVWATAASAYGWLKPSGLDTDSRGFVSVDGRLRSVSHPHV
                     FAAGDCAAMVDRALPRSGVYAVRQGPPLAENLRRTLAGMPLIRYSPQRRTLALISTGG
                     RHAVASWDGLALSGRWVWRWKDHIDRRFVARYQSTGQG"
     gene            complement(3942..>4301)
                     /locus_tag="AC158Q_04555"
     CDS             complement(3942..>4301)
                     /locus_tag="AC158Q_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339075.1"
                     /translation="FTFIGIVTPGLGWFLYFLLIPFWAMLPVVIFGSHVTFYILIGYL
                     AGFPLAKLLVSRSATYRKAAEDIKKKGVATVGGFTVGSAGGSWSSGSSWSSGGSSGGG
                     FSGGGGSSGGGGSSGSW"
BASE COUNT          785 a         1379 c         1401 g          736 t
ORIGIN      
        1 gtcgcttttc gagttcatct cgggcttcgg ggcttcctgc ttctcgtcgc tttgcgagat
       61 cagttcgggc ttcggcgctt cctgcttctc gtcgctttgc gagaccagct cgggcttcgg
      121 ggcctcctgc ttctcgtcgc tttgcgcgta ggtagccgtt gtcgaaagag cgaacgcgct
      181 gacaagcgcg agggccagtg cagtgttgcg tttcatgtaa ctctcctttt ccagtcaggg
      241 ttaaacgtcg gcgtcttgac caactgttgg ttggacgcgc gaagcataag agaggaacac
      301 gggctgagcg cattgttacc aacatgtaat ccgtgcgtag taccgccgta agttccggca
      361 cgaatcccgt cgcggaactt acggcggact tacaaacgcg ctgagggatg tcgctaagcg
      421 acgacggacg ggctgctgcg attggcttgc tggtgggatt tctgcgcggc tgcactggcc
      481 gcgtgaaagc ggacggtgac gcgaagtccg gggcgattgt cggacaactc taccgatgcg
      541 ccgtgcaggt cggccaccgc cttcacgagc gcgagcccga gtccactacc gggcgtcgtc
      601 cggctcgact caaggcggta aaagcgacgg aatactttct cgcgctccgg ttccggtatg
      661 cctggaccgg tatcggctac gctgagcacg gccatgccgg cttcctgccg gagatgaacg
      721 gtgattccga cgccgggcgg gcagtggcgg attggattct cgatgagatt ggccatcatc
      781 tgggtaagca gctcgcgatc accctgaatc gagggaacgg gatcaataat aatttcgagt
      841 tgctgggtgc ggtcctcagc caccggcacg tacgcgtcgg atacggcgga cgcgatctga
      901 gacaggttga catcggagaa acccgcgcgc ctcgcgccag cctcgatttg ggcgatacgc
      961 aacagcgcgg agaacgtctc caggatcgcg tcgagctccg aaatggattg ctcgatcacc
     1021 ggatcgccgt ccggtcccgc attgtcgcgg gccacctcga gccggtggcg cagccggctc
     1081 aagggcgtgc gcaagtcgtg cgcaatatca tcggaaaccc gcttcaggct ttccatcagc
     1141 gcctgaatgc ggccgagcat ggcgttcagg ttcaacgcca gcaggtccat ttcgtcgccg
     1201 ctgcccctcg tccgcatgcg ttgagagagg tcaccgtcca tgatggagcg ggtcgtgcga
     1261 ttgatttcct cgatgcggcg gagaaagccg ccgcgcagcg cggctccgcc gattcccgca
     1321 agcagcaccg tgatcgcgat gccccagctg aacgccctga cgatcgcctc ccgcgccttg
     1381 accacccggt agcggctttc acccacgagc aaaaactcgc cgttgtcgag acgctgtccc
     1441 tgggccacga cagggtaagc gtcggccgga ttgtccgctt tggcgctgcg gttcggatac
     1501 aggtacgagg cagggagcac cgtttccccc tcggtgggat ccatgggcgg gaggttgccg
     1561 gcgacgcgtt cgccgctgcc attctgcagc aggtaatacc ggcggggggc ccgcaagtcg
     1621 accactctca gctggatcgc gcgcgccacg ctgtcgaggc cgcggccgcg atacagctcg
     1681 accaggacct ggctttcacg ttcgatcgaa tcgctcagct gctcgtcgat cgcggcaagc
     1741 gcgatccagt aaacgacgac tagcaggacc gcgaccgatg ccccgaagac ggcggcatac
     1801 catgcgagaa gccggaagct cgtgctgcgg aagaagctat cttgagacac cgagggaata
     1861 tcccgaaccg cggacggtat gaaggagctc ctcgtcgaaa tcccggtcga ttttcgcgcg
     1921 caggcggctg atgtgggttt cgaccacgct ggtgcgcggg tcgaagtgaa attcccagac
     1981 gttctcaagc agcatggtgc gggtcaccac ctggccggca ttgcgcatca ggtattccag
     2041 caggcggaat tcctgcggct gcaactcgat ctgggtgccg ccgcgggtta ccgtgcgctt
     2101 cagccggttc atctcgagat ccccgacccg caggacggtc gcctcttccg ccatcggtgg
     2161 gcggcgcgcc agcgcgttga cccgggcaag cagctcggca aaggcgaatg gcttggtgag
     2221 gtagtcgtcg gcgccggcct ccaggccgtc gacgcgatcg ttgacgccgc ccagcgtggt
     2281 gagcaggagc accggggatc gggtccctgc cgcgcggatc gtcttcacga tggcgagccc
     2341 gtcgagaccg ggcagcatgc ggtcgatgac caggacgtcg tacccgccgc cagaaccgcg
     2401 caaaagtccg tcgcggccgt tgtccgccag gtcaacaacg tgtccatgct cgcgcagccc
     2461 gttcgccaca tacgccgagg tgtgcggatc gtcttcgatc agaagtatct tcatggaggt
     2521 cgtatggcgg caaaactgca ggcaatccgc cgcctgtatc gttgagcggc gccgaacgat
     2581 cccgtcgtct cgctgcaaag ctctgcggtg cggcaaatcc cccgccgggc tcctaagata
     2641 gcgcagcggc ccccgcctca caagcaacct gttgagccgg aagaccctgt ttaccaaatc
     2701 gtaatatcca ggcaatggcc cggaagatga gactactgcg ccgccgttcg cactgcgcct
     2761 tgtcacggga aaccgggggg ggtgcgacac gcccggcggc tcatccttga ccggtgctct
     2821 ggtagcgcgc cacgaagcga cggtcgatat ggtctttcca ccgccacacc caacggccgc
     2881 tcagggccaa tccatcccag gaagcgaccg cgtgcctgcc accggtgctg atcaaggcca
     2941 gcgtcctgcg ctgtggcgaa tagcgaatca gcggcatgcc cgccagcgtg cggcgcaggt
     3001 tctcggcaag cggcggcccc tgtctcacgg catagacgcc ggacctggga agggcacgat
     3061 ccaccatggc cgcgcaatcg ccggcggcaa acacgtgcgg gtgcgacacc gatcgcagcc
     3121 tgccgtccac ggatacgaaa ccgcgcgaat cggtgtcgag cccgctcggc ttgagccagc
     3181 cgtacgcgga ggccgcggtt gcccacacga tccagtcggc gcgaagactc gtgccatttt
     3241 caaagtgcac gatgccggcc tccactttca cggcgcggca ggcgcaatga agcgcgattc
     3301 cgcgctcggc aaacaggcgc tcgaagtcgc ggcgcacacc cggcggatga gattgaagga
     3361 tcgcgggcgc atcggtcacg acggacagcc gtgacgccgc ggcgccattg gattgcaggc
     3421 gatgatgcat cgcagccgcc agttccacgc cgccggcgcc gccgccgacg atggcgatgc
     3481 tttgcggaga atggcgcgct tccgcgagca ttttttccca cgcggcaagc aagacggcga
     3541 caggcttgac gctcacggcg aattgcggcg cgccgggcgc tgatgcggtg tccggtgtgg
     3601 agccgatatc gagagacagc aggtcgaagg cccgttcgcc gccgtcgcgg aatcgcgcgc
     3661 gattatgcgc aagttcgagc ccggtgactt cggcccgctc gaaagtcact cgagcggcgc
     3721 ggcacagcgc ctcgagatcg atatggcagt cgtggtagcc ataatgcccg gcgatcagcc
     3781 cgggcagcat gccggaatag gacgtgtatc gctcggggct gaccaggatg acatcggcgc
     3841 catgcggcgg agcggtgccg aagcggcgca ccacttctac gtggctgtgc ccgcccccga
     3901 ccaggagcag ccgcttcacg tcagacagat ctgggggcgg atcaccagct gccggaagag
     3961 ccgccgccgc cggagctgcc gccgccgccg gaaaacccgc cgcccgagct gccgcccgaa
     4021 ctccaggagc tgccggagct ccagctgccg cccgcgctgc cgaccgtaaa tccgccgacc
     4081 gtggcaacgc ctttcttctt gatgtcttcg gccgccttgc ggtaggtcgc cgagcgcgac
     4141 accagcagct tggcgagggg gaagccggcc aggtagccga tcaggatata gaacgtgacg
     4201 tgactgccga aaatcaccac cggtaacatg gcccagaacg gtatcagtaa aaagtagagg
     4261 aaccagccca ggcccggtgt gacgatgccg atgaatgtga a
//
LOCUS       DBNGSG010000176         5444 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_228615, whole genome shotgun sequence.
ACCESSION   DBNGSG010000176 DBNGSG010000000
VERSION     DBNGSG010000176.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5444)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5444)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5444
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_228615"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..761
                     /locus_tag="AC158Q_04560"
     CDS             141..761
                     /locus_tag="AC158Q_04560"
                     /inference="COORDINATES: protein motif:HMM:NF016819.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HIR3339076.1"
                     /translation="MPRTIRISIVAALAAAPWPAWAHHFMDGSLPQTFGQGLLSGLGH
                     PLIGVDHAAFIVAAGFLLALVRGGMWGVAALVAGSLAGAALHLAGVDLPGGEVGVALS
                     VILAGGLVMARRRIALPWLAGGMLLAGALHGHAYAEAIFGAETAPLAAYLIGFSAIQL
                     AVATAAFLAHRWLIAARAAWVKPVSSGLGAAAGAIGVVFLFLNLAG"
     gene            776..1138
                     /locus_tag="AC158Q_04565"
     CDS             776..1138
                     /locus_tag="AC158Q_04565"
                     /inference="COORDINATES: protein motif:HMM:NF045268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339077.1"
                     /translation="MSNPQPKKGAADIGGDDAGPVDTLDHGMKFWERQANGLRSVAQR
                     IKLVTTDELRRAAEDLGDEYAKLEYFERTTSALRTVLLEKGLITERELRAKMAEVRAR
                     FDVPRKKELPVRKKGAKR"
     gene            1135..1497
                     /locus_tag="AC158Q_04570"
     CDS             1135..1497
                     /locus_tag="AC158Q_04570"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3-like domain-containing protein"
                     /protein_id="HIR3339078.1"
                     /translation="MTAQAASGSKQPGAAPLPSTYTPAPGHRFEVGDRVAVKRRYPPG
                     HRRTPSYIRGKTGVIERICGAFPNPEELAYGFDGEPKKVLYRVRFLQKDVWPDYRGPA
                     RDLIEMEIFEHWLEPATD"
     gene            1645..2355
                     /gene="nthA"
                     /locus_tag="AC158Q_04575"
     CDS             1645..2355
                     /gene="nthA"
                     /locus_tag="AC158Q_04575"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HIR3339079.1"
                     /translation="MSEHSHDYYARKKRGHDHGHDHGDHAPIEERDEGPPSEFEIMSR
                     AMQELLEEKGVLTAEQVRRRMELFEEELPYRGSRVVAHAWTDPEFRTRLLADGKAAVA
                     EFGIDLEAERLIAVENTPDVHNVIVCTLCSCYPRALLGMPPTWYKSTNYRSRVVHEPR
                     AVLKEFGTVLPDRVTVRVHDSNADMRYVVIPMRPKGTEGWSEERLAQLLTRDTLVGVT
                     TPKVQGESRKAKGERNPA"
     gene            complement(2399..3517)
                     /gene="dinB"
                     /locus_tag="AC158Q_04580"
     CDS             complement(2399..3517)
                     /gene="dinB"
                     /locus_tag="AC158Q_04580"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HIR3339080.1"
                     /translation="MPPRFIAHLDMDAFYASAELLRYPELRGKPVVIGGRRRRAVEEA
                     AFPRLREYSGRGVITTATYEARAFGVNSGMGLMKAAKLAPDALLLPADFDEYRRLSRL
                     FKEAVRALAPEIEDRGIDEIYVDITRLVEDSAAEGAPDPWVRARVVARDLQRAVREAT
                     GLSCSIGVTPNKLLSKMASELEKPGGLTVLREEDIPARIWPLPVRKVNGIGPKAGARL
                     EALGIRTIGELARAAPALLVEHFGRSYGPWMHDAAHGRDGRAVVTYSEPKSISRETTF
                     EEDLHAVRDRERLSRIFTGLCVKLAEDLGRKGYVGRTIGLKLRYDNFRTVTRDLTLDA
                     PTGDAATIRRAAGECLKRVPLDRRIRLLGVRVGALSRA"
     gene            3675..4232
                     /locus_tag="AC158Q_04585"
     CDS             3675..4232
                     /locus_tag="AC158Q_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HIR3339081.1"
                     /translation="MTDPIAVWHAEHVRFARLLDFIEQQMTIFHDGGDPDYELMRDVL
                     YYLHHYADRYHHPREDVAFARLVQHEPGLKFAVNRLIQEHRVMAAVGETLLNYLEDIL
                     EDTVIERETVEAAAATYLVYYRHHLNTEEREVLPQAARFLRPDDWAAVASAVASAPDP
                     LVGDDVSERYRELRKRLSSTREKAN"
     gene            complement(4329..>5444)
                     /locus_tag="AC158Q_04590"
     CDS             complement(4329..>5444)
                     /locus_tag="AC158Q_04590"
                     /inference="COORDINATES: protein motif:HMM:NF024771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR3339082.1"
                     /translation="VFSSGSYDTGPADRLLGKPVLVKIHADGVTGLGQIRPISPGHFV
                     ADTTQSMVAAIKEIYGPALIGKSIFDIEAINEMFDLRLAGNPAARAVLDIAVRDAQGK
                     ALKTPLYNLIGGCCQPRIPLEWSVSMADKVAVMIAESQRAVDGFGIRVLCLKMADRRG
                     WRQDVKNFEAVRKAVGEDVMIGIDPNTGWTLADALSAIAEIRKMGLGYIEQPIERRDL
                     KGMAEIRRAAGGVPVMADEGIFTLQDAYALAEARACDAYCIKLYKLGGITAAKRIAAV
                     AQAANIQINCGGLAVQSQLEAAAGAHFYASIPAARMMGAAEFVMGLNATAKDPLCPES
                     DFRIRDGHVDVPAGPGLGVTIDEAALKKNTLLHERVG"
BASE COUNT          880 a         1896 c         1777 g          891 t
ORIGIN      
        1 ttcaaggttc aaggttcaag gttcaaaaag gtttggccgg tgtaccttga accttgttcc
       61 ttgcaccttg taactggttc ttccgggtat tcctcaaagt agtatattta ccttcgactg
      121 ccccgacccg gatggatccc atgccgagaa caatcaggat atccatagta gccgcgcttg
      181 cggcggcgcc ctggcccgcg tgggcgcatc acttcatgga cggcagcctg ccgcagacgt
      241 tcgggcaggg cctgctgtcg ggcctggggc atccgttgat cggagtcgac cacgcggcgt
      301 tcatcgtcgc cgcgggattc ctcctcgccc tggtcagggg cgggatgtgg ggcgtcgccg
      361 cgctggtcgc cggcagcctc gccggcgccg cactgcacct cgcgggagtt gacttgcccg
      421 ggggcgaggt cggcgtcgcg ctgtccgtga tcctggccgg cggcctcgtc atggcccggc
      481 gccggattgc gctgccgtgg cttgcgggcg gcatgctgct cgcgggcgcg ctgcacggac
      541 acgcctacgc ggaagccatc ttcggcgcgg agacggcgcc gctcgccgcc tatctgatcg
      601 ggttttccgc aatccagctg gccgtcgcga cggcggcctt cctcgcgcac cggtggctca
      661 tcgccgcgcg ggcggcctgg gtcaagccgg tctcctccgg gctcggcgcc gcggcgggcg
      721 cgatcggcgt ggtgttcctg ttcctcaacc tcgcgggctg aagccgggca tccccatgag
      781 caacccgcaa ccgaagaaag gcgcggccga catcggcggc gacgacgccg ggccggtcga
      841 cactctcgat cacggcatga agttctggga gcggcaggcc aacgggctgc gctcggtcgc
      901 gcagcggatc aagctggtca cgaccgacga gctgcgccgc gcggcggagg acctgggcga
      961 cgagtacgcg aagctcgagt atttcgagcg cacgacctcg gcgttgcgca cggtgctgct
     1021 cgagaaaggc ctcatcaccg agcgggaact gcgggccaag atggccgaag tccgcgcgcg
     1081 cttcgacgtg ccgcgcaaga aggagctgcc ggtcaggaag aaaggggcga agcgatgacc
     1141 gctcaggccg cttcgggcag caagcagccg ggcgcagcgc ccctgccctc cacctatacc
     1201 cccgcgcccg ggcaccgctt cgaggtgggc gaccgggtcg cggtcaagcg ccgctacccg
     1261 cccggtcacc gccgcacgcc cagctacatc cgcggcaaga ccggcgtgat cgagcgcatc
     1321 tgcggcgcct ttcccaaccc ggaagaactc gcctacggat tcgacggcga gccgaagaaa
     1381 gtgctgtacc gcgtgcgctt cctgcagaag gacgtctggc cggactaccg cgggcccgcg
     1441 cgcgacctca tcgagatgga aatcttcgag cactggctgg agccggcaac tgactagaac
     1501 ccgtcaaaaa caagaatgga caggatttac aagattaaca agattaaaac cgcattaaat
     1561 cctgctaatc ctgtctattc ggatcttgtg ttcatgggta ttcgagtatt ttgagacgca
     1621 ttccaatcac acggcagaac cgtcatgagc gagcacagtc acgactacta cgcgaggaag
     1681 aagcgcgggc acgatcacgg ccatgatcac ggcgatcatg cgcccatcga ggagcgcgac
     1741 gagggcccgc cgtcggaatt cgagatcatg agccgcgcca tgcaggagct gctcgaggaa
     1801 aagggcgtgc tcaccgcgga gcaggtgcgg cgccgcatgg agctgttcga ggaggagctg
     1861 ccctaccgcg gctcgcgcgt ggtcgcgcac gcgtggaccg acccggaatt caggacgcgc
     1921 ctgctggccg acggcaaggc cgcggtcgcg gagttcggca tcgacctgga agccgagcgc
     1981 ctgatcgcgg tggagaacac cccggacgtg cacaacgtca tcgtgtgcac gctgtgctcg
     2041 tgctatccgc gcgcgctgct cggcatgccg ccgacctggt acaagagcac caattaccgc
     2101 tcgcgcgtgg tgcacgagcc gcgcgcggtt ctgaaggaat tcggcaccgt gctgcccgat
     2161 cgcgtcaccg tgcgcgtgca cgactccaac gccgacatgc gctacgtggt gatccccatg
     2221 cgcccgaaag gcaccgaggg ctggagcgag gagcggctcg cgcaactgct gacgcgtgac
     2281 acgctggtcg gcgtcaccac gcccaaagtg caaggcgaaa gtcgaaaggc gaaaggcgaa
     2341 aggaaccccg cctaaccggt tttacttccg acttccgact ttcgcctttc gacttcgctc
     2401 aggctctgct gagcgccccc acccgcaccc cgagcagccg gatgcgccgg tcgagcggca
     2461 cgcgcttcag gcactcgccc gccgcgcggc ggatcgtggc ggcatcgccg gtcggcgcat
     2521 ccagcgtgag gtcgcgcgtg acggtcctga agttgtcgta gcgcagcttc aggccgatcg
     2581 tccggccgac gtagcccttg cgcccgaggt cctccgcgag cttcacgcac aggccggtga
     2641 agatccgcga gagccgctcg cggtcgcgca ccgcgtgcag gtcttcctcg aacgtcgtct
     2701 cgcggctgat ggatttcggc tcgctgtaag tgaccaccgc ccgcccgtcg cgcccgtgcg
     2761 cggcgtcgtg catccagggg ccatagctcc tgccgaaatg ctcgaccagc agcgccgggg
     2821 cggcccgggc cagctcgccg atggtgcgga tgccgagcgc ctcgagccgg gcgccggcct
     2881 tggggccgat gccgttcacc ttgcgcaccg gcagcggcca gatgcgcgcg gggatgtctt
     2941 cctcgcgcag cacggtcaat ccgccgggct tctccagttc ggaggccatc ttcgagagca
     3001 gcttgttcgg cgtgacgccg atcgagcagg acagcccggt cgcctcgcgc accgcgcgtt
     3061 gcaggtccct cgccaccacg cgggcccgca cccacgggtc cggcgcgccc tcggccgcgc
     3121 tgtcctcgac caggcgggtg atgtccacgt agatctcgtc gatgccgcgg tcctcgattt
     3181 ccggcgcgag cgcgcgcacc gcttccttga acaggcggga gagccgccgg tactcgtcga
     3241 aatccgccgg cagcagcagc gcatccggcg cgagcttcgc cgccttcatc agtcccatgc
     3301 cggagttcac gccgaatgcg cgcgcttcgt aggtggccgt ggtgatcacg ccccggccgc
     3361 tgtactcgcg caagcgtgga aacgcggctt cttccacggc acgccgccgc cgcccgccga
     3421 tcaccaccgg cttgccgcgc agctccgggt agcgcagcag ctcggccgac gcgtagaacg
     3481 cgtccatgtc gaggtgggcg ataaagcgcg gcggcatgcc gccagtgtac ccaagggcgc
     3541 ctgcccgggc ggcaggcgtc ccccttgtcg ccctgcacaa gccttgatcc tgatcaatag
     3601 cgcgccatgc cggcagccga caatgggatc gaccgtggcg cccgcccggg cgccggcttc
     3661 cagggagccc cgccatgacc gacccgatcg ccgtctggca tgccgagcac gtgcgtttcg
     3721 cgcgcctgct cgattttatc gagcagcaga tgactatctt ccacgacggc ggggacccgg
     3781 attacgaact gatgcgcgac gtgctctact acctccacca ctatgccgac cgctatcacc
     3841 atccgcgcga ggacgtcgcc tttgcccgcc tggtccagca cgaacccggg ctgaagtttg
     3901 cggtcaaccg gctcatccag gagcaccgcg tgatggccgc cgtcggcgaa acgctgctca
     3961 actacctgga ggatatcctg gaggacacgg tcatcgagcg cgagaccgtg gaggcggcgg
     4021 cggcgaccta cctggtgtac taccgccacc atctcaacac ggaggaacgc gaggtcctgc
     4081 cgcaagcggc ccgtttcctc agacccgacg attgggccgc cgttgcgtcc gccgtagcct
     4141 ccgcgcccga cccgctcgtc ggcgacgacg tgtccgagcg ctaccgggaa ctgcgcaagc
     4201 ggctcagttc aacacgggaa aaagcgaatt gaccctcgac ctgcatcccg caaggcgtaa
     4261 ggcgaaaagc ggaagcagaa atccctcttt ctactttcga ctttctcctt tcacctttcg
     4321 acttgtcttc agccgacgcg ctcgtgcagc agcgtgttct ttttcagggc ggcttcgtcg
     4381 atggtgacgc ccaggccggg gccggccggc acgtcgacgt gcccgtcgcg tatccggaaa
     4441 tcactttcgg ggcacagcgg gtccttcgcc gtggcgttga gccccatcac gaactcagcc
     4501 gctcccatca tccgggccgc gggaatgctc gcgtagaagt gggcgccagc ggccgcttcc
     4561 agctgcgatt gcacagcgag gccgccgcag ttgatctgga tgttcgcggc ctgcgcgacg
     4621 gcggctattc tcttcgcggc cgtgatgccg cccagcttgt agagcttgat gcagtaggcg
     4681 tcgcacgcgc gcgcttccgc cagcgcgtag gcgtcctgca gcgtgaagat gccctcgtcc
     4741 gccatcaccg gcaccccgcc cgcggcgcgg cggatctcgg ccatgccctt gagatcccgg
     4801 cgctcgatcg gctgctcgat gtagcccagc cccatcttcc ggatctccgc gatcgccgac
     4861 agggcgtcgg cgagcgtcca gccggtattc ggatcgatgc cgatcatcac gtcctcgccg
     4921 acggccttgc gcaccgcctc gaagttcttc acgtcctggc gccagccgcg gcggtccgcc
     4981 atcttcaggc acagcacgcg gatgccgaac ccgtccaccg cgcgctgcga ctcggcgatc
     5041 atcaccgcga ccttgtccgc catgctgacc gaccactcga gcggaatgcg cggctggcag
     5101 cagccgccga tcaggttgta gagcggcgtc ttcagcgcct tgccctgcgc gtcgcgcacc
     5161 gcgatgtcga gcaccgcccg cgccgccggg ttgcccgcga gccgcaggtc gaacatctcg
     5221 ttgatcgcct cgatgtcgaa gatggacttg ccgatcagcg cggggccgta gatctccttg
     5281 atggcggcca ccatgctctg cgtcgtgtcg gcgacgaaat gtcccgggct gatcgggcgg
     5341 atctggccca ggccggtcac gccgtcggcg tggatcttga ccagcaccgg cttgccgagc
     5401 aggcggtcgg ccggcccggt gtcgtaggag ccgctcgaga aaac
//
LOCUS       DBNGSG010000177         4452 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3794633, whole genome shotgun sequence.
ACCESSION   DBNGSG010000177 DBNGSG010000000
VERSION     DBNGSG010000177.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4452)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4452)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4452
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3794633"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="AC158Q_04595"
     CDS             <1..509
                     /locus_tag="AC158Q_04595"
                     /inference="COORDINATES: protein motif:HMM:NF013844.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HIR3339083.1"
                     /translation="VAGVTPLLHSKKVEFDRLLPRPVPAQRLRDNQEALKDSLSDPIP
                     WDAGLEAGDELSYARNGIGAQTLRRLRRGHWVTQAELDLHGLTVGEARDLLVEFLNQC
                     VRRGLRCIRIIHGKGLGSRNREPVLKRNVANWLMRRDEILAFCQARRAEGGSGAVVVL
                     LKGVRSKE"
     gene            complement(566..904)
                     /locus_tag="AC158Q_04600"
     CDS             complement(566..904)
                     /locus_tag="AC158Q_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HIR3339084.1"
                     /translation="MKKIEAIFKPFKLDEVREALSESGVSGLTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKVEVVVPDKLQESAIDAIVKSARTGKIGDGKIFVTSVEQVIRI
                     RTGETDEAAI"
     gene            complement(957..2648)
                     /locus_tag="AC158Q_04605"
     CDS             complement(957..2648)
                     /locus_tag="AC158Q_04605"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006415884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HIR3339085.1"
                     /translation="MKIAIAQINATVGDLSGNVARIAEFAGRAREQGADLMVTPELAL
                     CGYPPEDLLLREDFLAACDQALADLAGRAGGVTLVVGHPRAVDGKRYNSASVIRDGRV
                     TGVYDKQRLPNYTVFDEERYFEPGGKPCVFGIDGISFGVNICEDTWGAQGPVTARSRG
                     DYGDDRVGINICADSWNATAPRAAREAGANVLLVLNASPYHMEKQRVRYDVMRERVAE
                     NGMALVYANMVGGQDELVFDGGSFVLNRDGVLTHQLPLFREALGLVTLTDSDPQPGEI
                     VKPVSQDAEIYEALITGVRDYIDKNGFPGALIGISGGIDSALTLAVAVDALGAQRVRA
                     VMMPSQYTAGMSVEDALAEAKALGVRYTEIPIKPIFEVFLKSLAGEFKGLAADATEEN
                     LQSRIRGTLLMALSNKTGALVLTTGNKSEMGTGYATLYGDMAGGFAVLKDINKQLVYR
                     LADYRNSLSPVIPQRVIDRAPSAELRPDQTDQDSLPPYPVLDAIMEAYVEGDLSPREI
                     VAHGYAVEDVERVVRLVRASEYKRRQAPVGIRITPRGFGKDWRFPITNKFRHQFD"
     gene            2725..3861
                     /locus_tag="AC158Q_04610"
     CDS             2725..3861
                     /locus_tag="AC158Q_04610"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3339086.1"
                     /translation="MTIEVVDTLAGVPAGKWNSLTAGEPFLSHEFLSALHETGCAASA
                     SGWTPQFLLLRQDGALTGAMPLYLKSHSYGEYVFDWAWADAYHRHGIAYYPKLLSAIP
                     FTPVTGRRLLAATDADRDILIASALELARDLRVSSLHCLFPTRQEAQRMVAHGMMPRT
                     TVQFHWSNRGYASFAEFLAGFSHDKRKKVRQERRKVSDAGIRFKWLEGDEIQERDWAF
                     FNRCYRQTYREHGSSPYLNLEFFCRIGRALPRNIVLIVAQRNGNPVAASLNIRDRQRL
                     CGRHWGALEQHPALHFETCYYQGIEFCIARGIASFEGGSRGEHKLARGLMPVETCSAH
                     WLAHPEFAAAVGQFLTKETRGMERYVDELSEHSPYKRLHGNATQ"
     gene            complement(3885..>4452)
                     /locus_tag="AC158Q_04615"
     CDS             complement(3885..>4452)
                     /locus_tag="AC158Q_04615"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CE1 family esterase"
                     /protein_id="HIR3339087.1"
                     /translation="RRIPLDRARIYATGLSNGSMMAYRLAVEAPGHIAAVAGVAGAMT
                     LPGFGPGRPVPVMHIHSVDDPRALYSGGLGPAFQFTDTRVLHQSVDAMLALWIARNGC
                     SGRPRASDPISGRAGAADAKHTATRYIHAPCRDGTEVVLWKLTGAGHVWPGGVQDYLP
                     LLLGTGTTVIDANREIWKFFSRFRRNSR"
BASE COUNT          804 a         1473 c         1366 g          809 t
ORIGIN      
        1 cggtagcggg cgtgacgccg ttactccatt ccaagaaggt ggagttcgat cgcctgctgc
       61 cgcgcccggt gccggcacag cgcctgcgcg acaaccagga agcgctgaag gacagcctca
      121 gcgaccctat tccctgggat gccggcctgg aggccggcga cgaactttcc tatgcccgca
      181 atggcatcgg ggcgcaaacg ctccgaagac tgcgccgggg ccattgggtg acccaggcgg
      241 aacttgacct gcacggcctg acagtcgggg aagcgcgcga cttgctcgtg gaatttctca
      301 atcaatgtgt ccggcggggt ttgcgctgca tccgcatcat tcacggcaag ggcctgggct
      361 ccaggaaccg cgaaccggtg ctcaagcgca atgtggcgaa ctggctgatg cggcgagacg
      421 agattctggc tttctgccag gctcggcgcg ccgaaggcgg cagcggcgcg gtagtggtac
      481 tgctgaaagg tgtgaggagt aaggagtaag tagtgagggg gcgaggtgtt actccccgca
      541 cttcacgcct cgctgctcac ggtatctaaa tcgccgcctc gtcggtctcg ccggtccgga
      601 tgcggatgac ctgttcgacg ctggtaacga aaatcttgcc gtcaccgatc ttgccggtgc
      661 gcgccgactt gacgatggcg tcgatcgcgc tctcctgcag cttgtccgga accaccacct
      721 cgaccttgac cttcggcagg aaatccacga cgtactcggc gccgcggtaa agctcggtgt
      781 ggcccttctg cctgccgaag cccttcactt cggtgacggt caggccgctg acgccgcttt
      841 cggacaaggc ctcgcgcact tcgtctagct tgaacggctt gaaaattgct tcgatttttt
      901 tcatgagatt cctccgcttt ccagcaccgc gtatgtcata aggatatcac aggccgctaa
      961 tcgaactgat ggcggaactt gttggtgatc ggaaagcgcc aatccttgcc gaaaccgcgc
     1021 ggcgtgatgc ggatgccgac cggggcctgg cgccgcttgt attcgctcgc gcgcaccagc
     1081 cgcaccacgc gctcgacatc ctccacggca taaccgtgcg caacgatttc ccgggggctc
     1141 aggtcgcctt cgacataagc ttccatgatg gcgtcgagca ccgggtatgg cggcaggctg
     1201 tcctggtcgg tctggtccgg gcgcaattcg gccgacggcg cgcggtctat gacccgctgc
     1261 ggtatcaccg gggagaggct gttgcggtaa tccgcaagcc ggtataccag ctgcttgttg
     1321 atgtccttca gcaccgcaaa tccgcccgcc atatccccat agagcgtcgc gtaaccggtt
     1381 cccatctcgc tcttgttgcc ggtggtgaga accagcgcgc cggtcttgtt cgaaagcgcc
     1441 atcagcagcg tgccgcggat acgcgactgc aggttttctt ccgtggcgtc ggccgcaagc
     1501 cctttgaact ccccggcgag cgacttgaga aaaacttcga aaatcggctt gattggaatt
     1561 tcggtgtaac gcacgccgag cgccttcgct tcggccagcg catcctccac gctcatgccc
     1621 gcggtgtact gcgacggcat catgacggcc cgcacgcgct gcgcacccag ggcatccacg
     1681 gccaccgcca gggtcaacgc cgaatcgatg cctcccgata tgccgatcag cgcccccgga
     1741 aaaccgttct tgtcgatgta gtcgcgcacc ccggtgatga gcgcctcata gatttccgcg
     1801 tcctgcgaaa ccggcttgac gatttcgccg ggctgcggat cgctatcggt cagagtaacc
     1861 aggcccagcg cttccctgaa cagcggcaac tggtgcgtca gcacgccgtc gcggttcagc
     1921 acgaatgaac cgccgtcgaa caccagttcg tcctggcccc cgaccatatt ggcataaacc
     1981 agagccatgc cgttttccgc cacgcgctcg cgcatcacgt cgtaacgcac gcgctgtttt
     2041 tccatgtggt agggagacgc attcagtacc agcagcacgt tggcgccggc ctcgcgcgcg
     2101 gcgcgcggcg ccgtggcatt ccacgagtca gcgcagatat tgatgcccac gcggtcgtcg
     2161 ccgtagtctc cgcgagaacg cgcggtgacc ggcccttgcg cgccccaggt gtcttcgcag
     2221 atattcacgc cgaagctgat gccgtcgatg ccgaacacgc acggcttgcc gccgggctcg
     2281 aaatagcgct cttcgtcgaa cacggtgtaa tttggcaacc gctgcttgtc gtagacgccg
     2341 gtcacccttc catccctgat cacggaggcg gagttgtagc gcttgccgtc gacggcgcgc
     2401 ggatggccta ccacgagcgt gacgccgccc gcccggccgg caagatcggc cagggcctgg
     2461 tcgcaggcgg caagaaaatc ctcgcgcagg agcaggtctt ccggcgggta gccgcacaat
     2521 gcgagctccg gcgtcaccat gagatccgcg ccctgctcgc gcgcgcgccc ggcaaactcg
     2581 gcaatgcgcg cgacgttgcc gctcaggtcg ccgacggtgg cgttgatctg cgcaatcgca
     2641 attttcatgt cgctaatata tcatcgtgcc actctcgatg caggctgcag aaacccggcg
     2701 atcgttcgaa gaaacagtgc gaaagtgacg atagaagtcg tcgatacgct cgccggcgtg
     2761 ccggccggga agtggaacag cctcaccgcc ggcgagccgt tcctgagcca cgagttcctg
     2821 tcggcgctgc atgaaaccgg ctgtgcggcg agcgcgagcg gctggacgcc gcaatttctc
     2881 ctgttgcggc aagacggcgc cctgacggga gcgatgccgc tctacctcaa gtctcattcc
     2941 tacggcgagt acgtgttcga ctgggcatgg gccgatgcct atcaccgtca cggaatcgcg
     3001 tactatccca agctgctttc ggccatacca ttcacgccgg tcaccggcag gcgcctgctc
     3061 gccgcgacgg acgcggaccg agacatcctg attgcatcgg cgctcgaact tgcccgcgac
     3121 cttcgggtgt cttcactgca ctgcctgttc cccacccgcc aggaagcgca gcggatggtc
     3181 gctcacggca tgatgccgcg aacgaccgtg caatttcact ggtcgaaccg gggttacgcc
     3241 agcttcgcgg agtttcttgc cgggttcagc cacgacaagc gcaagaaagt ccggcaggag
     3301 cgccgcaagg taagcgatgc cggcatccgc ttcaagtggc tggagggcga cgagattcag
     3361 gagcgcgact gggccttttt caaccgttgc taccgccaga cctaccgcga gcatggttcc
     3421 agcccctatc tcaatctcga attcttctgc cgcatcggcc gcgccctgcc gcgcaacatc
     3481 gttttgatcg tcgcgcaacg caatggcaat cccgtcgcgg cctctctcaa cattcgcgac
     3541 cgccaaagac tctgcggccg tcattggggc gcgctggagc agcatcccgc gctgcatttc
     3601 gagacctgtt attaccaggg cattgaattc tgcatcgcgc gcggcatcgc gagcttcgag
     3661 ggcgggtcac gcggagagca caagctcgcg cgcgggttga tgccggtgga aacctgctcg
     3721 gcgcactggc tggcgcaccc ggaattcgcg gcggcagtcg ggcagttcct cacgaaagaa
     3781 acgcggggga tggaacgcta cgtcgacgaa ctctccgagc acagccctta caaacggttg
     3841 catggaaacg ccacgcaata ggcgccgcaa accccgcttg tgcctcaccg gctgttgcgg
     3901 cgaaatcgcg aaaaaaattt ccagatttca cgattggcgt cgatgaccgt agtgccggtc
     3961 ccgagcagca gcggcaggta atcctgcacg ccgccgggcc agacgtgccc cgccccggtc
     4021 agcttccaca gcacgacctc cgtcccgtca cgacacggcg catggatata gcgcgtcgcg
     4081 gtgtgcttcg cgtccgccgc gccggccctg ccactgatcg ggtctgacgc ccgcgggcgc
     4141 ccggaacacc cgttgcgggc aatccacaag gcgagcatgg cgtccaccga ctgatgcaat
     4201 acccgcgtgt cggtaaactg gaacgccggg ccgaggccgc cgctgtacaa tgcgcgtgga
     4261 tcatccacgc tgtggatatg catgaccggc accggccgtc ccgggccgaa tcccggcagc
     4321 gtcatggcac ccgccacgcc cgccacggca gcgatgtgcc ccggcgcctc gaccgccagg
     4381 cgataagcca tcatcgagcc gttggagagg cccgtggcat agatacgcgc gcgatcaagg
     4441 ggtatgcgcc gc
//
LOCUS       DBNGSG010000178         3899 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_738854, whole genome shotgun sequence.
ACCESSION   DBNGSG010000178 DBNGSG010000000
VERSION     DBNGSG010000178.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3899)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3899)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3899
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_738854"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="AC158Q_04620"
     CDS             <1..1137
                     /locus_tag="AC158Q_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990391 - DNA repair complex [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrA family protein"
                     /protein_id="HIR3339088.1"
                     /translation="GAGEHGGNVIAQGSPQDVEKHPESLTGQYLSGRRRIELPALRHR
                     VNPKRQLAVHGASGNNLKGISVGIPVGLFVCVTGVSGSGKSTLVNDTLHAAVARALND
                     SPVEPAPFESMTGVEQFDKVVNVDQSPIGRTPRSNPATYTGLYTPIRELLAGGPEARA
                     RGYGAGRFSFNVKGGRCEACQGDGVLKVEMHFLPDIYVPCDVCHGERYNRETLDIRYK
                     GRSVHEVLLMTVEQAAEFFSAVPAISRKLQTLLDVGLGYIKLGQSATTLSGGEAQRVK
                     LSLELSKRDTGRTLYILDEPTTGLHFHDIDLLLRVLHRLRDHGNTVVVIEHNLDVIKT
                     ADWVIDLGPEGGDEGGRVIAEGTPEDVARNKASVTGEYLREVLK"
     gene            1162..2463
                     /locus_tag="AC158Q_04625"
     CDS             1162..2463
                     /locus_tag="AC158Q_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase type-2 family protein"
                     /protein_id="HIR3339089.1"
                     /translation="MAKDTAPASPRELLLGSFQAAVAAADPLKIVPRHLPQSPKGRTL
                     VVGAGKGAAAMALAVEQNWPAKTPLDGLVVTRYQHGLLTNRVTVIEASHPVPDERGEQ
                     AAHEILRRVKGLTPDDLLLVLISGGGSALLSLPAEGLAMADLKATTRELLKCGAPIQD
                     MNTVRKHLSAIQGGRLAAASRAPVLALAISDVTGDDPTHIASGPCAPDPTTYQDALDV
                     LKRYDVKTPKAVVEHLARGAKGELPETPKPGSKIFKRVENRVVATAQASLQAAAGYFI
                     GHDIHPAILGDSVTGEAREVARVYGALAREVAQHGLPYQPPVALISGGETTVTVRGRG
                     RGGRNAEFLLALAVDLAGAPGIHALACDTDGIDGSEDNAGAVLAPDSLKRAAKKKLDA
                     AKRLDDNDGYGFFSALGDLVVTGPTRTNVNDYRVILVTNDG"
     gene            2566..3534
                     /locus_tag="AC158Q_04630"
     CDS             2566..3534
                     /locus_tag="AC158Q_04630"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3339090.1"
                     /translation="MVSRFVTPAAVLCLILAAGTTSAQTYPAGPMRIVVPFAAGGSTD
                     IMARTIAQQLGARFGQQVLVENRPGASGTIGTAFVAKSAPDGHTLLLVQASFVSNPSL
                     FKNLPYNQSRDLLPVTNLATGPLTLTVHPSLPAKTVRQLIALGKKYPGEINYGSPGAG
                     SISRLACALFNQMAGIRMTHIPYKGSGAAMADMLSGQVTFYAPNLFLSLPHIRAGKLH
                     VLGVTTAQRSPIAPDIPTIAESGLPGYEVSTWFGIFVPGATPRATVGQLNQALAQIVK
                     SRDMQERLARDGLTAVASTSEEFTKEVARDTDKYSKVIRALGIQAE"
BASE COUNT          651 a         1372 c         1292 g          584 t
ORIGIN      
        1 ggcgccggcg agcacggcgg caacgtcatc gcgcagggct cgccgcagga cgtcgaaaag
       61 cacccggaat cgctcaccgg ccagtacctg tcggggcgcc gccgcatcga gctgccggcg
      121 ctgcgccatc gcgtgaatcc gaagcgccag ctcgcggtcc acggcgccag cggcaacaac
      181 ctgaaaggca tcagcgtggg catcccggtg ggcctgttcg tgtgcgtgac cggcgtgtcc
      241 ggctcgggca agtctacgct cgtcaacgac acgctgcacg ccgcggtggc gcgcgcgctg
      301 aacgacagcc cggtcgagcc cgcgccgttc gagtccatga ccggcgtgga gcagttcgac
      361 aaggtggtga acgtggacca gagcccgatc gggcgcacgc cgcgctccaa tcccgccacc
      421 tacaccggcc tctacacgcc gatccgcgag ctcttggccg gcgggccgga ggcgcgcgcg
      481 cgcggctacg gcgccgggcg cttctccttc aacgtcaagg gcgggcgctg cgaggcctgc
      541 caaggcgacg gcgtgctcaa ggtcgagatg cacttcctgc ccgacatcta cgtgccgtgc
      601 gacgtgtgcc acggagagcg ctacaaccgc gagacgctcg atatccgcta caagggccgc
      661 agcgtccacg aggtgctgct gatgacggtg gagcaggcgg cggaattctt cagcgcggtg
      721 ccggcgatct cgcgcaagct gcagacgctg ctcgacgtgg gcctgggcta catcaagctg
      781 ggccagagcg cgacgacgct ctccgggggc gaggcgcagc gcgtgaagct ctcgctggag
      841 ctctccaagc gcgacaccgg gcgcacgctc tacatcctcg acgagcccac cacggggctc
      901 cacttccacg acatcgacct cctgctgcgc gtgctgcacc gcctgcgcga ccacggcaac
      961 acggtggtgg tgatcgagca caacctcgac gtcatcaaga ccgccgactg ggtgatcgac
     1021 ctcggccccg agggcggcga cgagggcgga cgcgtgatcg ccgagggcac gccggaggac
     1081 gtggcgcgca acaaggcgag cgtcacgggc gagtatctga gagaggtgct gaagtgactc
     1141 gtttcccgtt tctcgttgct cgtggctaag gacaccgcgc ccgcttcgcc tcgtgagttg
     1201 ctgctcggca gcttccaggc cgcggtagcc gctgccgacc cgctgaaaat cgtcccccgg
     1261 cacctgccgc aatcgccgaa ggggcgcacg ctcgtcgtcg gcgccggcaa gggcgcggcg
     1321 gccatggcgc tcgcggtcga gcagaactgg ccggcgaaaa cgccgctcga cggcctcgtc
     1381 gtcacgcgct accagcacgg cctgctcacc aaccgcgtta ccgtgatcga ggcgagccac
     1441 cccgttccgg acgagcgcgg cgagcaggcg gcgcacgaaa tcctgcggcg cgtgaagggc
     1501 ctgacgcccg acgatctgct gctggtcctc atctccggcg gcggctccgc cctgctctcg
     1561 ctgcccgcgg aaggcctcgc catggcggat ctcaaggcga ccacgcggga gctgctgaaa
     1621 tgcggcgcgc cgatccagga catgaatacg gtgagaaagc atctctccgc gatccagggc
     1681 ggccggctcg ccgctgccag ccgcgcgccg gtcctggcgc tcgcgatctc ggacgtgaca
     1741 ggagacgatc ccacgcacat cgcctccggc ccgtgtgcgc ccgacccgac gacctaccag
     1801 gacgcgctgg acgtgctgaa acgctacgac gtgaagacgc cgaaggcggt cgtggagcac
     1861 ctcgcgcgcg gcgcgaaggg cgagttgccc gagacgccga agccggggtc gaagatcttc
     1921 aagcgcgtgg agaaccgcgt cgtcgccacc gcgcaggcct cgctccaggc cgccgcgggc
     1981 tactttatcg gccacgacat tcatcccgcc atcctcggcg actccgtgac cggtgaagcg
     2041 cgtgaagtgg cgcgagtcta cggcgcgctc gcgcgggaag tggcgcagca cggactgccg
     2101 taccagccgc cggtagccct gatttccggc ggcgagacga cggtgaccgt gcgcggccgc
     2161 gggcgcggcg gccgcaacgc cgagttcctg cttgcgctcg cggtggacct cgccggcgcg
     2221 cccggcatac acgcgctcgc ctgcgacacc gacggcattg acggctcgga ggacaacgca
     2281 ggcgccgtcc tcgcgccgga ttcattgaag cgggcggcga aaaagaagct cgatgccgcg
     2341 aagaggctgg acgacaacga cggctacggc ttcttcagcg ccctcggcga cctcgtcgtc
     2401 accggcccca cgcgcaccaa cgtcaacgac taccgcgtca ttctcgtcac gaatgacggg
     2461 tgacaagtga cgagtgatga gccgctttaa cgctgtacct tgcgccttgt accttgtacc
     2521 ttgtaccttg taacttgtac ctgtggtaac tccggggagg tgcccatggt atcgagattc
     2581 gtcacgccgg cggcagtcct gtgcctcatc ctcgcggcgg gcaccacgtc cgcgcagacc
     2641 tatcccgcgg gccccatgcg catcgtggtg ccgttcgccg cgggcggctc caccgacatc
     2701 atggcgcgca ccattgcgca gcaactgggc gcgcggttcg gccagcaggt gctcgtggag
     2761 aaccggcccg gcgcgagcgg cacgatcggc accgcattcg tcgccaagag cgcgcccgac
     2821 gggcacaccc tgctgctggt gcaggcgagc ttcgtctcga acccgagcct gttcaagaac
     2881 ctgccttaca accagtcgcg cgatctcctg ccggtcacca acctcgcgac ggggccgctg
     2941 acgctgaccg tgcacccgtc gctgccggcg aagacggtca ggcagctgat cgccctgggc
     3001 aagaagtacc cgggcgagat caattacggc tcgcccggcg cgggctcgat ctcgcgcctc
     3061 gcctgcgcgc tcttcaacca gatggccggc atccgcatga cccacatccc gtacaagggg
     3121 tcgggcgcgg ccatggccga catgctgagc ggccaggtca ccttctacgc gccgaacctg
     3181 ttcctcagcc tgccgcacat ccgcgccggg aaactccacg tgctgggcgt aaccaccgcg
     3241 cagcgctcgc cgatcgcgcc cgacatcccg accatcgcgg aaagcggtct gcccggctac
     3301 gaggtttcga cctggttcgg catcttcgtg ccgggcgcga cgccgcgcgc gacggtgggc
     3361 cagttgaacc aggcgctcgc gcagatcgtg aagtcgcgcg acatgcagga gcggctggcg
     3421 agggacggtc tcacggcggt cgcgagcacg tccgaggaat tcacaaagga agtcgcgcgc
     3481 gataccgaca agtacagcaa ggtcatccgc gcgctcggca tccaggccga gtagcgccgt
     3541 cgtgaccggc ggtccgagat tgacgattgg cggttggcag aaagttgctc tcgtcgctcc
     3601 cgcgaaggcg ggagcccaga aaacagcttc acagacaatc taggactttc tgttcccccc
     3661 catcctacct tccccctcgc agggggaagg ggatacccgc ctgcgcgggg atgacgattt
     3721 gttcttgtga gccacgtttt tcaccaacct gtcaggacac ttctccgtcg ctgcgtcact
     3781 tcgtcaccgc gtcactgcat gtacatcccg ccgttgatca ggtagcactg gccggtgatg
     3841 tagcccgcgt tctcgctggc gaggtgcaac aggagcgcgg tcacttcctg cggccggcc
//
LOCUS       DBNGSG010000179         2951 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_229072, whole genome shotgun sequence.
ACCESSION   DBNGSG010000179 DBNGSG010000000
VERSION     DBNGSG010000179.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_229072"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="AC158Q_04635"
     CDS             <1..869
                     /locus_tag="AC158Q_04635"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3339091.1"
                     /translation="AREELCMPVAAVKNGDIYFEDSGSGELIVLMAGLGHGAAYFENA
                     VPLLSGLGRVVAIDPRGVGRSTSKTEIYSVESWAADVIELMDHLGAPSAHLVGSSLGA
                     CVALQAAVDFPQRVASLVAVAGFSELDRSLELNFRMRIGIIEQLGLGDVLAAHIAMWT
                     LGRDFINTDEGRAAVERLFASVRRNSPERYLAFIRAILAFGRLEPGQQAQPKLTERLR
                     EIRVPSCIIVGGQDILTPVSQSEQLASRIAGAELHVVPGCGHITFTERPEETSSLIRD
                     FLLRVIRKQGAS"
     gene            885..1799
                     /locus_tag="AC158Q_04640"
     CDS             885..1799
                     /locus_tag="AC158Q_04640"
                     /inference="COORDINATES: protein motif:HMM:NF020650.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3339092.1"
                     /translation="MSITLIENFRAVFYAPFYAASALGAYENEGLEVVIKPSSDAVAR
                     FVISGEGEVSWSGPLRLMNTLDKNPSAGVVAFCEIVGRDPFFLVGRAPRPSFRLQDLA
                     GMRVGTVSEVPTPWICLRHDLRLAGVDETGIERTPGATMAENAAALRAGRLDVVQVFE
                     PHAAALEADGAGHVWYAGASRGYTAYTTFNTTRAFLDRHPRTALQMCRAMYRTQKWIA
                     AHSDADLAEAIAAYFPDLPARLLASSCHRYRSLGVWNRTPLPAREGFEWLRGAALADG
                     LLRKSIPYEDCVDTRFAEQVVREDPPPL"
     gene            1956..2216
                     /locus_tag="AC158Q_04645"
     CDS             1956..2216
                     /locus_tag="AC158Q_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339093.1"
                     /translation="MQYLMVLFLIIGIIVLMIAAILGASYGIGRFLNTHEGVRADPAN
                     KDPCAQCQADREWYEGLPMWKQNVVTAWWLVNRYQWQQKGCQ"
     gene            2356..>2951
                     /locus_tag="AC158Q_04650"
     CDS             2356..>2951
                     /locus_tag="AC158Q_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339094.1"
                     /translation="MAVYWDESVMTEDHRSPDVLAEGDSWFSYWIPGNGNLIDRFNHD
                     IWKDRYTLLCLAYPGDEAIKMVDGRSRRALEETLRAYPTIKLLLFSGGGNDIAAKNLL
                     QLLRPDCSGAKHYRACFRDGQPGQRLAEIRHAYHELVAIRDSCQPGAVIVTHNYDYAV
                     LGRKLLWMAWLEPYMQLAGVPKKFRGDIVTVFIDGFEK"
BASE COUNT          523 a          908 c          974 g          546 t
ORIGIN      
        1 cggcccgcga ggagctttgc atgccagttg ctgcggtgaa gaacggcgat atctatttcg
       61 aggacagcgg ctccggcgag ctgatcgtgc tgatggccgg gctcgggcac ggcgctgcat
      121 acttcgagaa tgcggtgccg ctgctctccg gcctgggccg agtggtcgcc atagacccgc
      181 ggggcgtggg gcgctcgacg agcaagacgg agatctattc ggtggagtcc tgggcggcgg
      241 acgtgatcga gctcatggac catctcggcg cgccgagcgc gcacctggtc ggctcttcgc
      301 tcggcgcatg cgttgccctt caggccgcgg ttgacttccc gcagcgcgtg gcgtcgctgg
      361 tggcggtcgc cggattctcg gaactggacc gctcgctgga actcaatttc cgcatgcgca
      421 tcggaatcat cgaacagctc ggtttgggag acgtgctggc ggcgcacatc gcgatgtgga
      481 cgctcgggcg cgacttcatc aacaccgatg aaggccgggc agcggtcgag cggctgtttg
      541 cttcggtgcg ccgcaattcc ccggagcgct atcttgcgtt catccgcgca atcctcgcct
      601 tcgggcggct cgagcccggc cagcaggctc agccgaaact taccgagcgc ctccgcgaga
      661 ttcgcgtgcc ctcgtgcatc atcgtgggcg ggcaggacat acttacgccc gtgtcccagt
      721 ccgagcagct ggccagccgc atcgccgggg ccgaactgca cgtcgttccc ggctgcggcc
      781 acatcacgtt taccgaacgc ccggaagaga cgtcgtccct catccgggat ttcctgctca
      841 gggttatccg gaagcagggc gcgagctaaa cttcactgcg caatatgtct atcaccctga
      901 tcgagaattt ccgcgcggtg ttctatgcgc cgttctacgc ggcgtccgcg ctaggcgcct
      961 atgaaaatga ggggcttgaa gtcgtcataa agccttcatc ggatgcggtc gcgcgtttcg
     1021 tgatttccgg ggaaggcgag gtttcgtgga gcggaccgct gcgcctcatg aacacgctcg
     1081 acaagaaccc ctcggccggc gtggtcgcgt tctgcgagat cgtggggcgc gacccgttct
     1141 tcctggttgg gcgcgcgccc cgtccttcgt tccggctcca ggacctcgca ggcatgagag
     1201 tggggacggt ttccgaagtg cccactccct ggatatgcct gcggcacgat ttgcgcctcg
     1261 cgggagtgga tgaaaccggg atcgagcgga cgcccggcgc caccatggcg gaaaatgccg
     1321 ccgccttgcg cgcgggacgc ctggatgtgg tccaggtttt cgagccgcac gccgccgcac
     1381 tggaagccga tggagcgggg catgtctggt atgcgggcgc aagccggggg tacaccgctt
     1441 acaccacgtt caataccacc cgcgcattcc tcgatcggca ccctcggacg gcgctgcaaa
     1501 tgtgccgggc catgtaccgg acccagaaat ggatagctgc gcattcggac gccgaccttg
     1561 ccgaagcgat tgccgcctat tttccggacc tgccggcgcg cctcctggcc tcatcgtgtc
     1621 accgctaccg gtcgcttggc gtgtggaacc ggacaccctt gcctgcgaga gagggattcg
     1681 agtggctgcg cggagccgcg ctggccgacg gattgctgcg aaaatcgatt ccatacgaag
     1741 attgcgtcga tacgcggttc gcggaacagg tcgtgaggga ggatccgccc cctctttgag
     1801 ccatctgccc cttccacgtg cagaaagtca cacacagggg cggccgcggg aggtagagta
     1861 gcggcggtga cggcgctatt taacgacaac agccgcattc gtttgtcatt tcgcaggggt
     1921 gtcgctccgc aactccggga aaggaggaac accagatgca gtacctgatg gtcttgttct
     1981 tgataatcgg catcatcgta ctcatgatcg ccgcaatcct gggagcttcc tatggcatag
     2041 gaaggttcct gaacacccac gaaggcgtgc gggcggatcc agcgaacaag gacccgtgcg
     2101 cgcaatgcca ggccgatcgt gagtggtacg aaggactccc catgtggaag cagaacgtcg
     2161 tgacggcctg gtggctggtg aaccgctatc agtggcaaca gaaaggctgc cagtagccgc
     2221 ggaagcacgc gcgccgcgct cttcggcgta gagcgcattc tcgcggtgcc ggcggcagac
     2281 tggcgccggg cattgcggga gtcgtcgaac gtccgatagc gaccccgcag tcatcccgac
     2341 acgagaggag acgccgtggc ggtttactgg gacgaaagcg tcatgacgga ggaccatcgc
     2401 agcccggatg tgctggcgga gggggattcg tggttttcct actggatccc cggcaacggc
     2461 aatctcatcg accgcttcaa tcatgatatc tggaaggacc gctacaccct gctttgcctc
     2521 gcctacccgg gcgacgaggc gatcaagatg gtcgacggcc gcagtcgccg ggcgctcgag
     2581 gagacgctaa gggcctatcc gaccatcaag ctgttgttgt tcagcggcgg gggcaacgac
     2641 atcgcggcga aaaacctgct gcagctgctg cggccggatt gcagcggggc aaagcattac
     2701 cgggcgtgct tccgggacgg tcagcccggg cagcggctgg ccgaaatccg gcacgcctat
     2761 cacgaactcg tcgccattcg ggacagttgc cagcccgggg ccgtgatcgt cacccataac
     2821 tacgattacg ccgtgctcgg caggaaactg ctgtggatgg cctggcttga gccctacatg
     2881 cagctcgccg gcgtgccgaa gaagtttcgc ggggacatcg tcaccgtgtt catagacggt
     2941 ttcgagaaaa t
//
LOCUS       DBNGSG010000180         5635 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1927204, whole genome shotgun sequence.
ACCESSION   DBNGSG010000180 DBNGSG010000000
VERSION     DBNGSG010000180.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5635)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5635)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5635
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1927204"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3291)
                     /gene="mfd"
                     /locus_tag="AC158Q_04655"
     CDS             complement(<1..3291)
                     /gene="mfd"
                     /locus_tag="AC158Q_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HIR3339095.1"
                     /translation="MPQMELPLPGNRVRTPPLHGSSDALLIAQLARARRPLAVFTAAA
                     ADAQRLLEEIPFFGEGLKVHLLPDWETLPYDQLSPHQDLVSERLSTLHQLMQRAFDVA
                     IVPVTTALLRLAPVEYLAARSFFFKQGEKLPPEELRRQLVTAGYTHVTQVMAPGEYCV
                     RGGLIDLYPTGSALPYRIELDDDRVDSIRSFDVDSQRSIYKVSEVRLLPAREFPMDEE
                     GQTRFRRNFREKFEGDPSRSRIYKDVSKGVPGTGIEYYLPLFFDKTAVVTDYLPPETL
                     ACVQPGLQQAIEQFWSDTHARYAVLRGDRSHPVLPPHDLYQTADEFLGSIKPYARVEL
                     NLASTAPAEGYETHPSSLITHHSAALPPLAVERRADDPLHLLRSFSAAFEGRVLILAE
                     GAGRRETLLEYFNEHGLPPAVAQDYPQFAAASAPFMLGTGPLHGGFILSDPPLAVVTE
                     NELYAHQARPRRNRDGAQRISPEGMLRDLSEVKIGDPVVHESHGIGRYLGLLTMDLGE
                     GETEFLTLEYAGGDKLYVPVSSLHLISRYSGAQADAAPLHQLGSGQWEKAKKKAAKQV
                     RDSAAELLALYAQRAAREGHAFKLSVHDYEAFADGFPFEETPDQAAAIKATLDDLVSG
                     KPMDRLICGDVGFGKTEVALRAAFIAVADGKQVSVLVPTTLLAEQHFNTFSDRFAQVQ
                     DTWPEKIAELSRFRTGQETVAAIEGLVSGQIDIAIGTHKLLSRDVKFKNLGLVIIDEE
                     HRFGVRQKERLKALRAEVDVLTLTATPIPRTLAMSLEGLRDFSVIATAPERRLAIKTF
                     VARHSKGLIREAALRELKRGGQIYFLHNDIDTIQSAEASLARLLPEARIRVAHGQMRE
                     RDLEHVMRDFYQQRFNVLLCTTIIETGIDIPTANTIIINRADKFGLAQLHQLRGRVGR
                     SHHQAYAYLLLPDEGNITAAAKKRLEAIQMMEELGSGFFLAMHDLEIRGAGEVLGESQ
                     SGEMQEVGFNLYVRMLDAAVRSLRNGREPDVSAPLEVATEINLHVPALLPADYCSDVH
                     ERLVLYKRLANCETPQALEALQEELVDRFGLLPDPARALIECHRLRLLGKPLGLARID
                     ASESAIVLQ"
     gene            complement(3342..4913)
                     /locus_tag="AC158Q_04660"
     CDS             complement(3342..4913)
                     /locus_tag="AC158Q_04660"
                     /inference="COORDINATES: protein motif:HMM:NF015078.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3339096.1"
                     /translation="MLRLSYLPPLMVYAAYGVSGLTGIVGTFFVKDYLGLPASVLAAL
                     GFWAGIPFVLKMPLGHLVDLIWRWKSWLVFLGAGLIAMSLAIMAGLLTDRAAMTAIMP
                     AEAWFVLAALLSPVGYVIQDVVADAMTVEAVPRVDEHGQPVPPEARKLMNTTMQTLGR
                     VALISGLALVAALNLYLFADVEKLSEAEKLAVYRTIYLTAMVIPLISVAGVGFAFFLR
                     RRAFRQLIAQGFDPRKARELLLEPDNRPAPNWWILGGSLAFVAFSLGMGFSRLPYSQE
                     VIFTGSLAIIAFLIAKLTRELEPEARATLIGTAIVIFVFRAVPGPGEGLTWWMIDELR
                     FDQRFLAVLSLIASVLTLIGMFLFRRFMADHSIAYVVGFLTLVGFVLSLPILGLYHGL
                     HQWTAPLTGGVVDARFIVLVNTALESPLGQIAMIPMLAWIANSAPQKLKATYFAIMAS
                     FTNLALSASQLGTKYLNQAFTVTREVKDAATGAVTTPADYSQLGEILVVQLLIALALP
                     FTAILISRRTRFRSA"
     gene            complement(4919..>5635)
                     /locus_tag="AC158Q_04665"
     CDS             complement(4919..>5635)
                     /locus_tag="AC158Q_04665"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HIR3339097.1"
                     /translation="GYTFLFVFDNHAVNPALVANVPFDTLKDFAPVMLVGTAPYAIAA
                     HPSRPFRSFAEVVQAAKAKPDAISVGSIGNGTLAHLTIALAQQAGGFRINHIPYKGGG
                     PMTQDLIGGQVDLGIGSAALLTPHVRNGKVRAVATTGEKRAHTLPDVPTLLEQGIPGV
                     VVYAWWGILAPAGTPKPILDRFHAELQKALSLPDVRKTLTENMGMELVVSSPEALQKW
                     IVAEMQRWGKVVKDNNIRAD"
BASE COUNT          948 a         1789 c         1980 g          918 t
ORIGIN      
        1 ctggagcacg atcgcggact cggacgcgtc gatgcgcgca agaccgagcg gtttgccgag
       61 caggcgcagg cgatggcact cgatcagagc gcgcgcgggg tcgggcagca gcccgaagcg
      121 gtccaccaac tcctcctgca gcgcttcgag cgcttgcggc gtctcgcagt tggcgaggcg
      181 cttgtagaga acgagacgct cgtgcacatc gctgcaatag tccgccggga gcagcgccgg
      241 gacgtgcagg ttgatctcgg tcgcgacctc cagcggcgcc gagacatcgg gctcgcgacc
      301 gttcctgagc gagcgcaccg cggcgtcgag catgcgcacg tagaggttga agccgacttc
      361 ctgcatctcg ccgctctggg actcgcccag cacctctccc gcgccgcgga tctcgagatc
      421 gtgcatggcg aggaagaagc ccgagccgag ctcttccatc atctggatcg cctccagccg
      481 cttcttcgcg gcggcggtga tgttgccctc gtcgggcagc agcaggtagg cgtaagcctg
      541 gtggtgcgag cggccgacgc gcccgcgcag ctggtgcagt tgcgcgaggc cgaacttgtc
      601 ggcgcggttg atgatgatgg tgttggccgt cgggatgtcg attccggtct cgatgatggt
      661 ggtgcagagg agcacgttga aacgctgctg gtagaagtcg cgcatcacat gctcgaggtc
      721 gcgctcgcgc atctgtccat gcgcgacgcg gatgcgggct tcgggcagca gcctggcgag
      781 cgacgcctcg gcactttgaa tagtgtcgat gtcgttgtgc aggaaataga tctggccgcc
      841 gcgcttcagt tcgcgcagcg ccgcttcgcg gatgagcccc ttgctgtggc gcgcgacgaa
      901 ggtcttgatc gcgagccggc gctcgggggc ggtcgcgatc accgagaaat cgcgcaggcc
      961 ctccagcgac atcgccagcg tcctcggaat cggcgtggcc gtgagcgtca gcacgtccac
     1021 ttccgcgcgc agggccttca gccgctcctt ctgccgcacg ccgaagcggt gctcctcgtc
     1081 gatgatgacg agccccaggt tcttgaactt cacgtcgcgc gagagcagct tgtgcgtgcc
     1141 gatcgcgatg tcgatctggc cggagacgag cccctcgatc gcggccacag tctcctggcc
     1201 ggtgcgaaac cgcgacagtt ccgcgatctt ctccggccaa gtgtcctgga cctgcgcgaa
     1261 ccggtcggag aaggtgttga aatgctgctc ggcgaggagc gtcgtcggca ccagcaccga
     1321 gacctgcttg ccgtcggcga cggcgatgaa cgcggcgcgc agcgccacct ccgtcttgcc
     1381 gaagcccacg tcgccgcaga tcaggcggtc catcggtttt cccgagacga ggtcgtccag
     1441 cgtggccttg atcgccgccg cctggtcggg cgtctcctcg aacgggaagc cgtcggcgaa
     1501 ggcctcgtag tcatggacgc tcaacttgaa cgcgtggccc tcgcgcgccg cgcgctgcgc
     1561 gtagagcgcg agcagttccg ccgcggaatc gcgcacctgc ttcgccgcct tctttttcgc
     1621 tttctcccat tgcccgctgc cgagctggtg cagcggcgcg gcgtccgctt gcgcgccgct
     1681 gtagcgggaa atcaggtgca ggctggacac cggcacgtag agcttgtcgc cgcccgcgta
     1741 ctccagcgtc agaaactccg tttccccttc acccaggtcc atcgtgagga gcccgaggta
     1801 gcggccgatg ccgtgggact cgtgcactac cgggtcgcct atcttgacct cggacagatc
     1861 gcgcagcatg ccctcggggg aaatccgctg cgccccgtcg cggttgcggc gcgggcgcgc
     1921 ctggtgggcg tagagctcgt tttccgtgac gacggcaagg ggcgggtcgg acaggatgaa
     1981 gccgccgtgc agcgggccgg tgccgagcat gaacggggcg ctggcggccg cgaactgcgg
     2041 gtaatcctgc gcgaccgccg gcgggagccc gtgctcattg aagtattcga gcagggtctc
     2101 gcgccggccg gcgccctccg ccagtatcag cacgcggcct tcgaacgccg ctgagaacga
     2161 ccgcagcaga tgcagcgggt cgtctgcacg ccgctctaca gctaacggcg gtagagcggc
     2221 ggagtgatga gtgatgagtg atgagggatg agtttcgtaa ccctccgctg gcgccgtgct
     2281 ggccaagttc agttcgacgc gggcgtaggg tttgatggag ccgaggaact cgtcggcggt
     2341 ctggtagagg tcgtgaggcg gcaggacggg gtggctgcgg tcgccgcgca gcacggcgta
     2401 gcgcgcgtgg gtgtcgctcc agaactgttc gatcgcctgc tgcaatcccg gctgcacgca
     2461 tgcgagcgtc tccggcggca ggtagtcggt gacgacggcg gtcttgtcga agaacagggg
     2521 caggtagtac tcgatgccgg tgccgggcac gcccttggag acgtccttgt aaatgcggct
     2581 gcgcgagggg tccccctcga atttctcgcg gaaattgcgg cggaagcgcg tctggccttc
     2641 ctcgtccatc gggaattcgc gcgcgggcag cagcctcacc tcgctgacct tgtagatcga
     2701 gcgctggctg tccacgtcga agctgcggat gctgtccacc cgatcgtcgt cgagctcgat
     2761 gcggtagggc agcgcgctgc cggtcgggta gaggtcgatc agcccgccgc gcacgcagta
     2821 ctcgccgggc gccatcacct gcgtcacgtg cgtgtagccg gccgtgacga gctggcggcg
     2881 caactcctcg ggtgggagct tttctccctg cttgaagaaa aacgaacggg cggcgagata
     2941 ttccaccggc gcgagacgca ggagcgccgt cgtgaccggc acgatcgcga cgtcgaacgc
     3001 gcgttgcatg agttgatgca gcgtggagag ccgctcggac accaggtcct ggtgcgggga
     3061 gagctggtcg tagggcagcg tttcccagtc gggcaggaga tggaccttga ggccctcgcc
     3121 gaagaacggg atttcctcca gtaggcgctg cgcatccgcc gcggcggccg taaagacggc
     3181 gagcgggcgc cgcgcgcggg cgagctgcgc gatcaggagc gcgtcggagg agccgtgcag
     3241 gggaggcgta cgtacacggt tccccggcag cggcagttcc atctgcggca tggtcggggg
     3301 aagtacttga aaatccagcg ttattatacc gtctggaggg gtcaagcgga gcggaatcgg
     3361 gtgcggcgcg agatcaggat cgcagtgaag ggcagggcga gcgcaatcag cagttgcacg
     3421 acgaggattt cgcccagctg gctgtagtcc gcgggcgtcg tgacggctcc ggtcgcggca
     3481 tctttcacct cgcgggtcac ggtaaaggcc tggttgagat acttggtgcc gagctgggaa
     3541 gccgacagcg cgaggttggt gaacgaggcc atgatggcga agtaggtggc cttgagtttc
     3601 tgcggggccg agttcgcgat ccacgccagc atcgggatca tcgcgatctg accaagcggc
     3661 gactccagcg ccgtgttcac cagcacgatg aagcgcgcgt ccaccacgcc cccggtgagt
     3721 ggtgcggtcc actggtgcag cccgtggtag aggccgagga tgggcagaga cagcacgaag
     3781 cccacgaggg tcagaaagcc cacgacatag gcgatcgagt gatcggccat gaagcggcgg
     3841 aacaggaaca tgccgatcaa cgtcagcacg ctggcgatca acgagagcac cgcgaggaag
     3901 cgctggtcga atcggagctc atcgatcatc caccacgtga gcccctcgcc ggggccggga
     3961 acggcgcgaa agacgaagat cacgatcgcg gttccgatca gcgtggcgcg cgcctcgggc
     4021 tcgagctccc gggtgagttt cgcgatcagg aacgcgatga tggccaatga cccggtgaag
     4081 atcacttcct ggctgtacgg caggcggctg aagcccatgc cgagcgagaa cgcgacaaag
     4141 gcgaggctgc cgccgaggat ccaccagttc ggcgccgggc ggttgtcggg ttccaacagg
     4201 agttcccgcg ccttgcgcgg atcgaagcct tgcgcaatca gttgccggaa tgccctgcgc
     4261 cgcagaaaaa atgcgaagcc gaccccggcg accgaaatca gcggaatcac catcgcggtc
     4321 aggtagatgg tccggtagac cgccagtttt tccgcttccg acagtttctc cacgtcggcg
     4381 aacagataga gattcaacgc cgccaccagc gccagcccgc tgatcagcgc gacccgtccc
     4441 agcgtctgca tcgtggtatt catgagcttg cgcgcttccg ggggcacggg ctggccatgc
     4501 tcgtccacgc gcggcaccgc ttccaccgtc atcgcgtcgg ccaccacgtc ctggatgacg
     4561 tagccgaccg gggagagcag cgccgccagc acgaaccagg cctcggccgg catgatggcg
     4621 gtcatcgcgg cgcggtcggt gaggaggccg gccatgatgg cgagactcat cgcgatcagg
     4681 ccggcgccga gaaacacgag ccagctcttc cagcgccaga tgagatcgac caggtggccg
     4741 agcggcatct tgagcacgaa cggaatgccg gcccagaagc cgagcgcggc gagaacggac
     4801 gcgggcaggc cgaggtagtc cttgacgaag aacgtgccga cgatcccggt cagcccggac
     4861 accccgtacg ccgcatacac catcagcggc ggcaggtacg acaggcgcaa caaagggatc
     4921 agtcggcgcg gatgttgttg tccttcacca ccttgcccca gcgctgcatc tcggcgacga
     4981 tccatttctg cagcgcctcg ggcgagctca cgacgagctc catgcccatg ttctcggtga
     5041 gcgtcttgcg cacgtccggg aggctcaggg ccttctgcag ctcggcgtga aaccggtcca
     5101 ggatcggctt gggcgtgccg gccggcgcga ggatgcccca ccacgcgtac acgacgacgc
     5161 cggggatgcc ctgctcgagg agcgtcggca cgtcgggaag cgtgtgcgcc cgcttttcgc
     5221 cggtggtcgc caccgcccgc accttgccgt tgcgcacgtg cggcgtgagc agcgcggccg
     5281 agccgatgcc gaggtcgacc tggccgccga tcaggtcctg ggtcatcggc ccgccgccct
     5341 tgtacgggat gtggttgatg cggaagccgc cggcctgctg cgccagcgcg atcgtcaggt
     5401 gcgcgagcgt gccgttgccg atcgagccga cgctgatcgc gtcgggcttg gccttcgcgg
     5461 cctgcaccac ctcggcgaaa ctgcggaacg gccgcgaggg atgcgccgcg atcgcgtagg
     5521 gcgcggtgcc gacgagcatc accggcgcga aatccttcag cgtgtcgaac ggcacgttcg
     5581 ccaccagcgc cgggttcacc gcgtggttgt cgaacacgaa aaggaaggta tagcc
//
LOCUS       DBNGSG010000181         6815 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_229628, whole genome shotgun sequence.
ACCESSION   DBNGSG010000181 DBNGSG010000000
VERSION     DBNGSG010000181.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6815)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6815)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6815
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_229628"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="AC158Q_04670"
     CDS             <1..550
                     /locus_tag="AC158Q_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HIR3339098.1"
                     /translation="VIPTTENMPGGRATRPGDIVKSLSGQTVEILNTDAEGRLILCDA
                     LTYAERFQPAAVIDIATLTGACVIALGHVATGLFANDDALAREVTSAGETAYDRVWHM
                     PLWDDYQEQLKSNFADFANIGGRPAGAVTAACFLARFTRKFKWSHLDIAGTAWKSGRE
                     KGATGRPVPLLTQFLVNRVGRK"
     gene            654..1082
                     /locus_tag="AC158Q_04675"
     CDS             654..1082
                     /locus_tag="AC158Q_04675"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF016267.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HIR3339099.1"
                     /translation="MTQIDFYTHVEDKLRTACQLSAKAFARGLKVTVFCPDPETAGRF
                     DRLLWSAHPIGFVPHCAPDDPLAGETPVIVDCRGDRLLHDEVLINLRPEWPSFFGRFQ
                     RLIEIVSREDDDRATARSRFRFYRDRGYEIRTHDLSKTGA"
     gene            1744..4551
                     /locus_tag="AC158Q_04680"
     CDS             1744..4551
                     /locus_tag="AC158Q_04680"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HIR3339100.1"
                     /translation="MALAKGFEPAAIEGRWYSIWEKNGYFQAGLDESNPNRFCILLPP
                     PNVTGTLHMGHAFQHTLMDALTRYHRMRGFNTLWQPGTDHAGIATQIVVERQLEQEGT
                     SRQALGRDAFVDRVWKWKEESGSTITRQMRRLGASCDWGRERFTMDEGLSRVVTEVFV
                     RLYENGLIYRGKRLVNWDPVLRSAVSDLEVESEEESGQLWHIRYPLESGAAFLIVATT
                     RPETMLGDVAVAVHPEDARYKHLVGERVLLPLTGRRIPVIADEYVDPQFGTGCVKITP
                     AHDFNDYQVGLRHKLEPIGIFNLDASVNDNAPPAYRGLDRYRAREKVLEDLKDQGLIV
                     EARLHKLMVPRCGRTGAVVEPMLTDQWFVKMDTLARSALDAVGRGEVRFVPANWTTTY
                     NQWLTHIQDWCVSRQLWWGHRIPAWYDGEGNAYVGRNRQEAQAKHRQAMLERVRAGGK
                     VTPSDLKQDEDVLDTWFSSALWPFSTLGWPADNPALRLYLPTSVLVTGFDIIFFWVAR
                     MVMMTLPFTGKVPFSEVYITGLVRDGEGQKMSKSKGNIIDPLDLIDGIGLDALVEKRT
                     SSLMDPRQAESIAAATRRQFPGGIPAFGTDAVRFTFASLASHGRDIKFDLSRCDGYRN
                     FCNKLWNAARYVLMNCQGKDTGIDETLPAELSVADRWITSRLQRAEAEAHQAFRDYRF
                     DNLSRSIYELVWDEYCDWYVEFAKVQLSGQDAQERGPNEARQRATRRTLTRVLESMLR
                     LAHPVIPFITEELWQQVAPLAGKKGASIMLSRYPEPQAEKIDEQAELDVALLKDLTNA
                     CRTLRSEMNVPPSQKLPLLIQGEPSRLAPFVPYLTALARLTEVMIVEGALPAAGAPVS
                     IVADYRLMLKIEVNMSAERERLHRERLRVEGEIEKCDVKLGNSSFVERAPPLIVAQER
                     ERLARFRAMLDKLNEQLKNLS"
     gene            4664..5911
                     /locus_tag="AC158Q_04685"
     CDS             4664..5911
                     /locus_tag="AC158Q_04685"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3339101.1"
                     /translation="MIHSLRAFRHRNYRLFFYGQSLAVLGNWIQHLAMSWLVYRLTGS
                     AWLLGVTGFASQIAILVLAPFGGLWADRIDRRALLLWTQAAAMVPGFVLAALAYAEVI
                     EIWHVVVMAGLFGIIMAIDVPVRQSFTPEMVPAREDLPSAIAFNALMQNSGRMLGPTI
                     AGLLIAASSEAVCFLVNGVSKIAAVATLAMMTVTPTARPRASFSIWGSLKEGVAYTWN
                     SVPLRLLFPVVAVVSFMASPYQTLMPIFAAEVFAGGADTLGFLIGAAGMGGMIGMLLL
                     ASRRDVRGLMNWIAASALIAGAALVVFAYSTSLALSLVMIAIAGLTSLTVAMGVSTIV
                     QTIVDDRMRGRVLGLFVVAFLGMFPLGSLAAGAAADWIGATHTLAAGGIACMLVALWL
                     WRRLPELRSHIRPIYVKLGIINE"
     gene            complement(6001..6702)
                     /locus_tag="AC158Q_04690"
     CDS             complement(6001..6702)
                     /locus_tag="AC158Q_04690"
                     /inference="COORDINATES: protein motif:HMM:NF022041.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR3339102.1"
                     /translation="MIRLRQFRPAFGLPNASPFCMKVETYLRMAGLAYECPRGADVRK
                     SPKGKMPYIEDEGAIVADSAFIIDYLKRKYGDALDSHLGVAERAAALACQRMLEENTY
                     WAVLYFRWIDEAGWKITRDAFFGWMKPPLKWIVPPLARRIVRRELHGHGMGRHARDEI
                     VVIGKKDLTAAADFLGDKAFFMGAQPSSLDAAAYAFLANVLWVPVESPLKAHARQYPR
                     LEAYCRRMKARYYGQ"
BASE COUNT         1189 a         2285 c         2183 g         1158 t
ORIGIN      
        1 cgtcatcccg acgaccgaga acatgccggg cggccgcgcc acgcgcccgg gcgacatcgt
       61 caagagcctg tccggccaga ccgtcgaaat cctcaacacc gatgccgaag gccgcctgat
      121 cctgtgcgac gcgctgacct atgcggagcg tttccagccc gccgcggtca tcgatatcgc
      181 cacgctcacc ggtgcctgcg tgatcgccct gggtcatgtc gccaccggcc tcttcgccaa
      241 cgacgacgcg ctcgcgcgcg aggtgaccag cgcgggcgag accgcctacg acagggtgtg
      301 gcacatgccg ctgtgggacg attatcagga gcagctgaag agcaacttcg ccgacttcgc
      361 caacatcggc gggcgccccg ccggcgcggt gacggcggca tgcttcctgg cgcgcttcac
      421 caggaaattc aaatggtcgc acctcgatat cgccggcacg gcctggaaaa gcggccggga
      481 aaagggcgca accggccgcc ccgtgccgct cttgactcag ttcctggtca accgggtcgg
      541 gagaaaatag tgttgagtgt tgagtgctta gtgttgagtt gctttgcctt ttctgtgtca
      601 ccaggcttca actcaacgct caaaactcaa cacttaaaac tcgcggagcg tatgtgacgc
      661 agatcgactt ctacacccac gtcgaagaca agctgcgcac cgcctgccag ctgtcggcca
      721 aggcgttcgc gcgcggcctc aaggtcaccg tattctgccc cgacccggaa accgcgggcc
      781 gattcgaccg gctgctgtgg tcggcgcacc cgatcggttt cgtgccgcat tgcgcgcccg
      841 atgatccgct ggccggcgag acgccggtca tcgtcgattg ccggggcgac aggctgctgc
      901 acgacgaggt gctgatcaac ttgcgcccgg aatggccgtc cttcttcggc cgcttccagc
      961 gcctgatcga gatcgtcagc cgcgaggacg acgaccgcgc cacggcccgc agccgtttcc
     1021 gtttctaccg cgaccgcggc tacgaaatcc gcacgcacga cctcagcaag accggtgctt
     1081 agtgtcggat tttgggaaat cgactgacaa gcgaagcgaa agcatgacaa ctcatacctc
     1141 accgctcatc acccgtcact gcgaagcctg atggccgagc taccgttacc acgcgacgat
     1201 ggcgacgacc tgctgaagcg cgccgacgcg ctgctcgcgc ggcaccgcgg cgcagccaag
     1261 cccgcggcaa cggcggcggc tcccgatgtc ccggtgctca ccgagcccac cgcgcaacca
     1321 gtatctgacg atgacatccc caccctcacc gaggtcatcc cggctgaact gctgcctgcc
     1381 gtcctcgcgg tccccggccc ggcggtggtg tccggtgaaa tcgtctcgcg cgtccaggcg
     1441 cagaacctcg agcacagcgt ctaccagaag ctgaagcgcg acctcgacca gcacatcgag
     1501 cgggtcgtgc aagagcgctt catgccgggc atcggcagcg cgctcgatgc ggcgctcgcc
     1561 aggatcagcc tggacatcaa gaccgacatc aacagcatgg tgcgtgcctc gatcgaggag
     1621 acgctgcgca cccagatcaa gaatctaggg gttgcggtgg aagccaaggc ggcgatgcgg
     1681 gaggcaacgg ccgcggtctc cagtataatg cccggctttt ccggttcgac ttctccatcc
     1741 gatatggcgc tcgccaaggg tttcgagccg gcggcgatcg agggccggtg gtactccatc
     1801 tgggagaaga acggctactt tcaggccggg ctcgacgaat ccaatcccaa ccgattctgc
     1861 atcctgctgc cgccccccaa cgtcacgggc acgctgcata tgggccacgc gttccagcac
     1921 acgctgatgg acgcgttgac gcgctatcac cgcatgcgcg ggttcaacac cctgtggcag
     1981 cccggcactg atcacgccgg catcgccacc cagatcgtag tggagcgcca gctcgagcag
     2041 gaaggcacct cgcgccaggc gctcggccgc gatgcgttcg tcgaccgcgt atggaaatgg
     2101 aaggaggaat ccggatccac tatcacgcgc cagatgcgcc ggttgggagc ctcatgcgac
     2161 tggggacgcg agcgcttcac catggacgag ggattgtcgc gggtggtgac ggaagtgttc
     2221 gtcaggctct acgagaacgg tcttatctac cgcggcaagc ggctggtcaa ctgggatccg
     2281 gtgctgagaa gcgcggtgtc cgacctcgag gtcgagtccg aggaggagtc cgggcagctg
     2341 tggcacattc gctacccgct ggaatccggc gccgctttcc tgatcgtcgc caccacccgg
     2401 cctgaaacca tgctgggcga cgttgcggtc gcggtacatc ccgaagacgc ccgctacaag
     2461 catctcgtcg gcgagcgggt gctgctgccg ctgacgggac gccggatccc ggtgatcgcg
     2521 gacgagtacg tggacccgca gttcggcacc gggtgcgtga aaatcacccc ggcgcacgac
     2581 ttcaacgatt accaggtcgg gctgcggcac aagctcgagc cgatcggcat cttcaacctg
     2641 gatgcgagcg tgaacgacaa tgcgcccccc gcctaccgcg gactcgatcg ctatcgcgcg
     2701 cgcgagaaag tgctcgagga cctgaaggac cagggcctca tcgtcgaagc aaggctgcac
     2761 aagctgatgg tgccgcgctg cggccgcacg ggggccgtcg tcgagccgat gctgaccgac
     2821 cagtggttcg tgaagatgga cacgctcgcc aggtcggcgc tggacgccgt ggggcgtggg
     2881 gaagtcaggt tcgtacccgc gaactggacg acgacttaca accagtggct cacccacatc
     2941 caggactggt gcgtctcgcg ccagctgtgg tggggccacc gcatcccggc ctggtacgac
     3001 ggcgagggca acgcctacgt cgggcgcaac cggcaggagg cccaggccaa gcaccgccag
     3061 gccatgctcg agcgcgttag agcgggcggc aaggtgacgc cctcggacct gaagcaggac
     3121 gaggacgtgc tcgacacctg gttctcctcc gcgctgtggc cgttctccac gctgggctgg
     3181 ccggcggaca acccggcgct gcggctgtac ctgccgacga gcgtgctggt caccggattc
     3241 gacatcatct ttttctgggt ggcgcgcatg gtgatgatga cgctgccttt caccggcaag
     3301 gtgccgttca gcgaggtgta catcacgggg ctcgtgcgtg acggcgaagg ccagaagatg
     3361 tccaaatcga agggcaacat catcgaccct ctcgatctga tcgacggcat cggcctcgat
     3421 gcactcgtcg aaaaacgcac gtccagcctg atggacccgc gacaggcgga atcgatcgcc
     3481 gccgccaccc gtcgccagtt cccgggcggc atccccgcgt tcggcaccga cgcggtgcga
     3541 tttaccttcg ccagcctcgc ctcgcacggt cgcgacatca agttcgacct ctcgcgctgc
     3601 gacggctacc gcaacttctg caacaagctg tggaacgccg cgcgctacgt gctgatgaat
     3661 tgccagggca aggacaccgg catcgatgag acgctgccgg cggagctatc cgtcgccgat
     3721 cgctggatca cgagccggct gcagcgcgcg gaagccgagg cgcatcaggc gttccgcgac
     3781 taccgcttcg acaatctttc gcgctcgatc tacgaactcg tgtgggacga gtactgcgac
     3841 tggtatgtcg agttcgcgaa agtgcagctg tcaggacagg acgcgcagga gcgcgggccg
     3901 aacgaggccc ggcagcgcgc cacgcggcgc acgctgacgc gcgtgctgga gtcgatgctg
     3961 cgcctagcgc acccggtgat cccgttcatc accgaggagc tgtggcagca ggtggcgccg
     4021 ctcgcgggaa aaaaaggcgc ctccatcatg ctctcccgct accccgagcc ccaggcggag
     4081 aagatcgacg agcaggccga gctcgacgtc gcgctgctga aggatcttac caacgcctgc
     4141 cgcacgctgc gttccgaaat gaacgtgccg ccgtcccaga agctgccgct gctgatccag
     4201 ggcgaaccgt cgcggctggc gccattcgtg ccgtacctga ccgcgctggc gcggcttacc
     4261 gaagtgatga tcgtcgaggg cgcgctgccg gccgcgggcg ccccggtttc catcgtggcc
     4321 gattaccgcc tgatgctcaa gatcgaggtc aacatgtcgg ccgagcgcga gcggctgcac
     4381 agggaacggc tgcgggtgga aggcgagatc gagaaatgcg acgtgaagct cggaaattcc
     4441 agttttgtcg agcgcgcgcc gccgctgatc gtcgcccagg agagagagcg actcgcgcgg
     4501 ttcagggcca tgctcgacaa gctcaacgag caactgaaga atctctcgtg acgggctgac
     4561 cgcggccctc gaccatccgc gcaggcctga aatgagggga tactcccctc atgctcccct
     4621 gcaccagggc ccgtatcttt caagccgatg cggatttcag gcaatgatcc attcgctgcg
     4681 cgcattccgt caccgcaact accggctgtt cttctacggc cagtcgcttg ccgtcctcgg
     4741 caactggatc cagcacctcg ccatgagctg gctggtctac cggctcaccg gctcggcctg
     4801 gctcctcggc gtaaccggtt ttgccagcca gatcgcgata ctcgtgctcg ctcccttcgg
     4861 cggcctgtgg gccgaccgca tcgatcgcag ggcgctgctg ctctggacgc aggccgcagc
     4921 catggtgccg gggttcgttc tcgccgcgct cgcctatgcg gaagtgatcg agatctggca
     4981 cgtcgtcgtc atggccggcc tgttcggcat catcatggcg atcgacgttc cggtgcggca
     5041 gtcgttcacg cccgaaatgg tgccggcccg cgaggacctt ccaagcgcca tcgctttcaa
     5101 cgcgctgatg cagaacagtg ggcgcatgct cggccccacc atcgcgggcc tgttgatcgc
     5161 cgcctccagc gaggcagtgt gctttctcgt caacggcgtg agcaagatcg cggcggtggc
     5221 gacgctcgcg atgatgacgg tcacgccgac tgcgcgcccc cgcgcctcgt tctcgatatg
     5281 gggcagtctg aaggagggtg tcgcctacac ctggaactcc gtgccactgc gcctgctctt
     5341 ccccgtcgtc gccgtggtga gcttcatggc gagcccgtac cagaccctga tgccgatctt
     5401 cgccgcggaa gtctttgccg gcggtgcgga tacgctcgga ttcctgatcg gcgcggccgg
     5461 catgggcggc atgatcggga tgcttctgct cgcgtcgcgc agggacgtgc gcggcctgat
     5521 gaactggatc gccgcgtcgg ccctcatcgc cggcgccgcg ctggtggtat ttgcctactc
     5581 gacctcgctt gcgttgtcgc tcgtcatgat cgcgattgcc ggcctgacct cgctgaccgt
     5641 ggcgatgggc gtatccacca tcgtccagac catcgtcgac gaccgcatgc gcgggcgcgt
     5701 gttgggcctg ttcgtcgtgg ccttcctcgg catgttcccg ttgggcagcc ttgccgccgg
     5761 ggctgcggcg gactggattg gcgccacgca cacgctcgcc gcaggcggta tcgcctgcat
     5821 gctggttgcg ctctggctat ggcggcggct gcccgagctg cgcagccaca tccggccgat
     5881 ttacgtgaag ctgggaatca tcaacgagtg aggtgtgggg agagaggggg gaggagcaaa
     5941 agcgatgcgc gcgccagcgc aattgcatgg ccctggccct cgcgcttaac gcctcacccc
     6001 tcactgcccg taataacggg ctttcatccg ccggcagtag gcttccagtc gcggatattg
     6061 ccgcgcgtgc gccttcaacg gggactcgac cggcacccaa agcacgttgg cgaggaaggc
     6121 gtacgccgcc gcatccagcg acgaaggctg cgcgcccatg aaaaacgcct tgtcgccgag
     6181 aaagtcggca gctgccgtga ggtccttctt cccgataaca acgatctcat cgcgcgcatg
     6241 ccgccccatg ccgtgcccgt ggagttccct gcgcacgatg cgccgggcga gcggcggcac
     6301 gatccacttg agcgggggct tcatccagcc gaaaaaggcg tctcgggtta tcttccagcc
     6361 ggcctcgtcg atccagcgga aatacaacac ggcccagtag gtgttctcct cgagcatgcg
     6421 ctggcaggcg agcgcggccg cccgctcggc cacgcccagg tgcgagtcga gcgcatcgcc
     6481 gtacttccgc ttcaggtaat cgatgatgaa ggccgagtcg gcgacgatcg cgccttcgtc
     6541 ctcgatgtag ggcatcttgc ctttcggcga cttgcgcacg tcggcgcccc gcgggcattc
     6601 gtaagcaagc cccgccatgc gcaggtaggt ttccaccttc atacagaacg ggctcgcatt
     6661 cggcagcccg aaagcgggac ggaattgacg aagccggatc aatcgcttac ctcgtttgcg
     6721 gcgacacccg gcacgcggcg ggcagaacct tctcgggcgc cgttcccggc cggcacagcc
     6781 agctcttgcc gccgtccgcg gaaagcaacc gcacg
//
LOCUS       DBNGSG010000182         6575 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2946627, whole genome shotgun sequence.
ACCESSION   DBNGSG010000182 DBNGSG010000000
VERSION     DBNGSG010000182.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6575)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6575)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6575
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2946627"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="AC158Q_04695"
     CDS             complement(<1..707)
                     /locus_tag="AC158Q_04695"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3339103.1"
                     /translation="MDTAAETPKDKRVEIAIHHWAPRFVSSGVPLTDFEEVTAGISRW
                     DEWCAAWGARAAVHEELGKKALADGYKLSAGEHLTRAAVLYHFGKFMFVHDLPQMKQA
                     HRKVIECRELALPHLPYPGERVQIPYQGGHLAGSLRKPAGGGRHPVVVMIVGLDSTKE
                     EMDVYENIFLARGMATLAFDGPGQGEAEYDIPIRGDYEVAVAAVVDYVGTRPDLDAKR
                     VGLWGVSLGGYYSARAAA"
     gene            complement(710..1678)
                     /locus_tag="AC158Q_04700"
     CDS             complement(710..1678)
                     /locus_tag="AC158Q_04700"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3339104.1"
                     /translation="MTRNLGITLFAALFAATGPATAAEQYPTKPIRLMVPFPPGAASD
                     FLARVVGQRMSEVYGQQAVVDNRPGAGGLIGSQIVARALPDGYTIGLVGQPHLSNTLL
                     REPHPYDPLRDFASVTLVAVIPNVIVLGNGVPATNVKDLIALAKAKPGQLNFGSAGVG
                     SSSHLAGEMMVAAAGIKAVHVPFKTIADIFSEMIAGRVHFYVFPLPAVMPVLKEGRLK
                     AIATATPKRAPALPGVPTTAEAGFGQYQSESWFGFVTPAGVPKRLITRLNADIGKILR
                     QPDIRERFQQGGAEATHGTPEQFHNLQREEYARLAKLIKTLGIRVQ"
     gene            complement(1711..2802)
                     /locus_tag="AC158Q_04705"
     CDS             complement(1711..2802)
                     /locus_tag="AC158Q_04705"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3339105.1"
                     /translation="MKDPRVQSAIAHWAPRFVSNGVPLADFEEVTAGVERWEDWCRAW
                     SARAAVHEELGREALAGNCRLTAGEHLTRAAVCYHFAKFVFVVDAVQMIAAHAKAVEC
                     RNLALPLISPPGERVAIPYEGRHLYGILRKPAGNREGMRPAVRPPVFVMCMGLDSTKE
                     EMDANESVFLARGMATLAFDGPGQGEGEYDFAIRGDYEIPVSAVVDYVENRGDLDGGR
                     VGLWGVSLGGYYAPRAAAFEKRARACIALSGPFEWAEFFDRLPPLTRDTFRVRSKSAS
                     EAQARERAATLTLKDVAKNITCPLFVVAGKQDGIVPWQEGERLAAVASGPVVRCFIED
                     GNHVANNRVYRYRSQSADWMARQLGAAAI"
     gene            2937..3650
                     /locus_tag="AC158Q_04710"
     CDS             2937..3650
                     /locus_tag="AC158Q_04710"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR3339106.1"
                     /translation="MRVTGKDIAATRTIRAREDGAQRIRDTILAAAADGSLEPGARLP
                     TERELVRRFDVPRNAVRRTLAQLEAEGSITRHVGRGTFLAGRADEETLTAASATHTSP
                     AELMEARLRIEPALAELIVTNATASDFERMEACLERAEKAATLDEFELWDAALHEALA
                     EATHNRFVIRVLDMVTVVRQQSEWGKLKDRIVTPERRIRYQQEHRDIVRALKARDAER
                     ARASIIAHLQHARRNLFGF"
     gene            3769..4365
                     /locus_tag="AC158Q_04715"
     CDS             3769..4365
                     /locus_tag="AC158Q_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HIR3339107.1"
                     /translation="MDVSFISAVVILLLVLDPIGNIPLFLSLLKHVEPGRRARVVLRE
                     CAIAYAVLILFVLFGPSILGIFGISDSSLTIAGGVILFLIALRMIFRNPDGLFGDVPR
                     GEPFIVPLAIPLIAGPTAIATVMLLVSREPQRLPEWAAVVTVAMMVTVVPLLFAERIA
                     KWVGEQGLIAFERLLGLILTAIAVEMLLRGIEIFVHQL"
     gene            complement(4969..>6575)
                     /locus_tag="AC158Q_04720"
     CDS             complement(4969..>6575)
                     /locus_tag="AC158Q_04720"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HIR3339108.1"
                     /translation="PYRLFALSNFASLLALLSYPVLIEPFLPLAAQSWLWSIAYAGFI
                     MLCGVTAYASTRGPAAAPAAPGHAGSAAEEAPAARRLLLWIALSATGSCLLLAVTNHL
                     TQNIASIPFLWVVPLSLYLLTFLLCFDHPRWYHRGVFLPLAAVLMPVMAWFSDSLELW
                     IATPLYAAGLFVCCMFCHGELTRVKPGPRYLTTFYLMVSVGGALGALLVGIAAPNLLS
                     GYYELGITLTVCAVLLYYRTLSSQWWVAGASLAVVVATAMFTVINIGDYLSNARVKLR
                     NFYGVVRTRDYPTPVPFRVMYHGAINHGGQLLDPEMRNTPTSYFAPSSGYGRTFASLP
                     DGPRRVGVIGLGAGAIAAYARKGDHFRFYEIDPQVAAVAVSEFTFLRDSPAQTDIVLG
                     DGRLSLEREPDQRYDLLAIDAFSGDSIPMHLITREAMATYVRHIVPDGAIVFQATNRF
                     VNIALVVERLAAEFGMTAVLVSDAPDNDEGANYWVSGTDQIIVTKSARLLAADPISSV
                     AERLPPRPDFRVWTDDFYNLLLILKR"
BASE COUNT         1111 a         2227 c         2184 g         1053 t
ORIGIN      
        1 gccgccgcgc gcgccgagta atagccgccc agggagacgc cccacaggcc cacccgcttc
       61 gcgtcgaggt ccggccgggt cccgacgtaa tccactaccg cggccaccgc cacttcgtag
      121 tcgccgcgga tgggaatgtc gtactccgcc tcgccctggc cgggaccgtc gaaggcgagc
      181 gtcgccatgc cgcgcgcgag gaagatgttc tcgtagacgt ccatctcctc cttggtcgaa
      241 tcgaggccca cgatcatcac caccaccggg tgccgtccgc ccccggcggg ctttctaagg
      301 ctgccggcga gatgcccgcc ctggtacggg atctgcacgc gctcgccagg gtagggcagg
      361 tgcggcaagg cgagctcgcg gcactcgatc accttgcggt gcgcctgctt catctgcggc
      421 aggtcgtgca cgaacatgaa cttgccgaaa tggtagagga ccgctgcgcg cgtgaggtgc
      481 tcgcccgcgg acagcttgta gccgtcggcc agcgctttct tgccgagctc ctcgtggacc
      541 gcggcgcggg cgccccacgc cgcgcaccac tcgtcccagc gcgagatgcc ggcggtcact
      601 tcctcgaaat cggtcagcgg cacgccgctc gagacgaagc gcggcgccca gtggtggatc
      661 gcgatctcga cgcgcttgtc cttcggggtt tcggccgccg tgtccacgat cattgcaccc
      721 tgatgccgag cgtcttgatg agcttggcga ggcgcgcgta ctcctcgcgc tgcaggttgt
      781 ggaactgctc cggcgtgccg tgggtcgcct ccgcgccgcc ctgctggaaa cgctcgcgga
      841 tgtccggctg ccggaggatc ttcccgatgt cggcattcag gcgggtgatc agccgcttcg
      901 gcacgccagc cggtgtcacg aacccgaacc aggattcgga ctggtactgc ccgaaccccg
      961 cctccgccgt ggtgggaact ccgggcaggg ccggggcgcg cttcggcgtt gccgtcgcga
     1021 tcgccttcag ccggccctcc ttcagtaccg gcatcaccgc cggcagcggg aagacgtaga
     1081 agtgcacgcg cccggcgatc atctcgctga agatatcggc gatcgtcttg aacggcacgt
     1141 gcacggcctt gatgccggcc gcggccacca tcatttcgcc cgcgagatgc gaggagctgc
     1201 ccacgccggc cgagccgaaa ttcagctgcc ccggcttggc cttcgcgagc gcgatgagat
     1261 ccttgacgtt ggtcgcgggc actccgttgc cgagcacgat gacgttggga atgacggcca
     1321 ccagcgtaac cgaggcgaaa tccctgagcg gatcgtacgg gtgcggctcg cgcaacagcg
     1381 tgttcgagag gtgcggctgc ccgaccagtc cgatcgtgta gccatcgggc aatgctctcg
     1441 cgacgatctg gctgccgatc agcccgcccg cgccgggacg gttgtcgacc accgcctgct
     1501 ggccgtagac ctccgacatg cgctggccga ccacgcgcgc caggaagtcg ctcgccgccc
     1561 ccggcggaaa cggcaccatg agccggatcg gtttggtcgg atactgctcg gcggcagttg
     1621 ccgggccggt cgccgcgaaa agtgccgcaa aaagcgtgat gccaagattg cgagtcatat
     1681 gccctcctcc ccttgtgcaa acctgcgttt tcagatcgcc gccgccccga gctgccgcgc
     1741 catccagtcg gcagactggg aacgataccg gtaaacgcgg ttgttggcga cgtgattgcc
     1801 gtcttcaata aagcatcgca ccaccgggcc cgaggccacc gcggcgagcc gctcgccctc
     1861 ctgccacggc acgatcccgt cctgcttgcc ggcgacgacg aacagcgggc aggtgatgtt
     1921 cttcgccacg tccttcaggg tcagcgtggc cgcgcgctcg cgggcctgcg cctcggacgc
     1981 cgacttcgag cgcacccgga aggtgtcgcg ggtgagcggc ggcagccggt cgaagaattc
     2041 cgcccactcg aacggacccg acagcgcgat gcacgcccgg gcgcgcttct cgaacgccgc
     2101 ggcgcgcggc gcgtaatagc ccccgagcga aacgccccat aacccgacgc gtccgccgtc
     2161 caggtccccg cggttctcca cgtagtccac gacggcactg accgggattt cgtaatcgcc
     2221 gcggatggcg aaatcgtatt cgccctcgcc ctggcccggc ccgtcgaagg cgagtgtggc
     2281 catgccgcgg gcgagaaata cgctctcgtt ggcatccatc tcttccttgg tcgaatcgag
     2341 ccccatgcac atcacgaaga cgggaggccg caccgcaggg cgcattccct cccggttgcc
     2401 ggcgggcttg cgcaggatgc cgtagaggtg ccgtccttcg tagggaatgg cgacgcgctc
     2461 gccgggcggc gagatgagcg gcagcgcgag attgcggcat tcgaccgctt tcgcatgcgc
     2521 cgctatcatc tgcacggcgt caacgacgaa gacgaacttg gcgaagtggt agcagacggc
     2581 ggcgcgcgtc aggtgctcgc cggccgtcag ccggcaattc cccgcgaggg cctcgcgacc
     2641 cagttcctcg tgaactgcgg cgcgcgcgct ccaggcacgg caccagtcct cccagcgctc
     2701 gacgccggca gtcacttcct cgaaatcggc cagggggacg ccgttggaga cgaagcgcgg
     2761 cgcccagtgc gcgatcgcag actgtacccg ggggtccttc atatggtctg agcgcggcgc
     2821 tccgcgaaga aaatacccgt ggccgggcgt tgcgcaatcg gtgggtcata ctaggcaagg
     2881 agtgaaccaa ttgtcaacaa agtagaccaa tttcaacaat tggtttatgt tagatcatgc
     2941 gcgtgaccgg gaaagacatc gcggcaaccc gcaccatccg tgcgcgcgag gacggcgcgc
     3001 agcgcatccg cgacaccatc ctcgccgcgg cggcagacgg ctcgctcgag ccgggcgccc
     3061 ggctgccgac cgagcgcgaa ctcgtgcggc gcttcgacgt gccgcgcaat gcggtgcggc
     3121 ggacgctggc ccagctcgag gccgagggct cgatcacgcg ccacgtcgga cgcggcacct
     3181 tcctcgccgg gcgagcggac gaagagacgc tcaccgccgc cagcgcgact cataccagtc
     3241 cagcggagct gatggaagcc cggctgcgaa tcgagccggc gctcgccgag ctgatcgtca
     3301 ccaacgccac cgcctccgac ttcgagcgca tggaggcctg cctcgaacgc gcggagaaag
     3361 ccgccacact ggacgagttc gagctgtggg atgcggccct gcacgaagcg cttgccgagg
     3421 ccactcacaa ccgcttcgtc atccgcgtgc tcgacatggt gaccgtggtg cgccagcaat
     3481 ccgagtgggg gaagctgaag gaccgcatcg tcactccgga gcgccgcatc cgctaccagc
     3541 aggagcaccg cgacatcgtg cgcgcgctga aggcgcgcga cgccgagcgc gcccgcgcga
     3601 gcatcatcgc ccacctgcag cacgcccgcc gcaacctctt tggcttctaa aggcgtgaat
     3661 cgtgattggt gattcgactc cagccctgcc ttgccccccg caaccggggg aatgaggtcc
     3721 aataagtcca ttcaccattc actattcacg aatcacgcct ctccaaccat ggacgtttcc
     3781 ttcatctccg cggtcgtgat cctgctgctg gtgctggacc cgatcggcaa tatcccgttg
     3841 tttttgtcgc tgctgaagca cgtcgagccg gggcggcgcg cccgcgtggt tctgcgcgag
     3901 tgcgccatcg cgtacgcggt gctcatcctg ttcgtgctgt tcgggccctc gattctcggc
     3961 attttcggga tctccgattc ctccctcacc atcgccggcg gcgtgatcct gttcctcatc
     4021 gcgctgcgca tgattttccg caatcccgat ggcttgttcg gcgacgtgcc gcggggcgag
     4081 ccgttcatcg ttcccctggc catccccttg atcgccggtc cgacggccat cgcaacggtc
     4141 atgctgctgg tgtcccggga gccccagcgc ctgccagagt gggccgcagt ggtgaccgtc
     4201 gccatgatgg taacggtggt cccgttgctg ttcgcggagc gcatcgcgaa atgggtcggc
     4261 gagcagggat tgatcgcctt cgagcgcctg ctggggctga ttctcaccgc gatcgcggtg
     4321 gagatgctgc tgcgcggcat cgagatcttc gtgcaccaac tctaagagcg ccaggccggg
     4381 gcggcggcca tgggcagcgc ggccggcctg cccccctgac ccagatatgc ccgcaaggcc
     4441 gcgccagacg ggcgttttca gcccatcccg cagcaaacgg gcgccatgtt gatatccccc
     4501 gaccccgcgg ccgctcgcat cgggcaaagc ccggtctgac gggcacactc atgccatgtg
     4561 tttgccgggc gtaatgcgcc tgggcggcaa gcgcaggatg agttcagtgc cggggataac
     4621 ggcgctgcgg cactgcgcag ctagtgcatt gattcggaac ccggatgctg agcagaacgg
     4681 acggtccggc cagcgccgcc tgccccgtcg cgtgactgtc accgcggtcc aggctggcgt
     4741 ggcaggcgca tcccgccgtt atcggttccc ggtccggtca aaaataattc acaatttttg
     4801 tggataacgt tgtgggtaac ccgccggtac gggcgcgaat acggcgaaat gacgggctcg
     4861 cgtctggatt gcacaaattt tcatcaacag cggcaagcgc taattcgact caggcgcctc
     4921 gggaagtttc cgcctggtcc ccatcgctca ccgcccgctg cttgctgatt accgtttgag
     4981 gatcagcagc aggttgtaga aatcgtcggt ccagacgcgg aaatccgggc gcggcggcag
     5041 ccgctccgcc accgaactga tcggatcggc ggccagcagc cgcgcgctct tcgtgacgat
     5101 gatctggtcg gtgccggaca cccagtagtt ggcgccctcg tcgttgtccg gcgcgtcgga
     5161 gaccagcacc gcggtcatgc cgaactcggc ggcaaggcgc tccaccacca gcgcgatgtt
     5221 cacgaagcgg ttggtggcct ggaatacgat cgcgccgtcg ggaacgatat ggcgcacgta
     5281 ggtcgccatc gcctcgcgcg tgatgaggtg catcggtatc gaatcgccgg agaaggcgtc
     5341 gatcgcgagc aggtcgtagc gctggtcggg ctcgcgctcg agcgagagcc gcccgtcgcc
     5401 gaggacgatg tccgtttgcg ccgggctgtc gcgcaggaac gtgaattcgg agaccgcgac
     5461 cgcggcgacc tgggggtcga tctcgtagaa gcggaagtga tcgcctttgc gcgcataggc
     5521 ggcgatcgcc cccgcaccca gcccgatcac gcccacccgg cgcggtccgt ccggcaggct
     5581 cgcaaacgtg cgcccataac cgctcgaggg cgcgaaatag ctggtcgggg tgttgcgcat
     5641 ctccgggtcg agcagctgcc cgccgtggtt gatggcgccg tgatacatga cgcggaacgg
     5701 taccggggtc ggatagtcgc gggtgcggac cacgccgtag aaattgcgca gcttgacgcg
     5761 cgcgttggac aggtaatcgc cgatattgat caccgtgaac atggcggtcg ccaccaccac
     5821 ggcgagcgaa gcgccggcga cccaccattg gctggaaagc gtgcggtagt agagcagcac
     5881 cgcgcacacc gtgagcgtga tgcccagctc gtagtagccc gagagcaggt tgggcgcggc
     5941 gatgcccacc agcagcgccc cgagggcgcc cccgaccgac accatcaggt agaaagtggt
     6001 caggtagcgc ggcccgggct tcacccttgt caactcgccg tggcagaaca tgcagcacac
     6061 gaaaaggccc gccgcgtaga gcggcgtggc aatccacagt tcgagcgaat cgctgaacca
     6121 cgccatcacc ggcatcagca ccgccgcgag cggcaggaac acgccgcggt gataccagcg
     6181 cgggtgatcg aagcacagga ggaaggtgag gaggtagagc gagagcggca ccacccacag
     6241 gaacgggatc gaggcgatgt tctgcgtcag gtggttggtg accgcgagca gcaggcacga
     6301 gcccgtcgcc gagagcgcga tccacagcag cagccgcctg gcggccggtg cttcctccgc
     6361 tgcgctgccg gcgtgcccgg gcgccgcggg cgcggcggcg ggtccgcggg tgctcgcata
     6421 agcggtcacg ccgcacagca tgatgaagcc ggcgtaggcg atggaccaga gccacgattg
     6481 cgcggcgagc ggcaggaagg gctcgatcag caccgggtag gaaagcagcg cgagcagcga
     6541 ggcgaaattc gacagcgcga acagccggta cggca
//
LOCUS       DBNGSG010000183         6984 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2947298, whole genome shotgun sequence.
ACCESSION   DBNGSG010000183 DBNGSG010000000
VERSION     DBNGSG010000183.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6984)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6984)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6984
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2947298"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="AC158Q_04725"
     CDS             complement(<1..894)
                     /locus_tag="AC158Q_04725"
                     /inference="COORDINATES: protein motif:HMM:NF022892.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2950 domain-containing protein"
                     /protein_id="HIR3339109.1"
                     /translation="MISIQFNCSRIVQRGAIAAIALAGMLACATSATAADAKQKTYAT
                     PEAAVKELVAAIRSGDEKAMLGILGPGSRDIVRSGDAVADKAGRERFVKAFEAANKLE
                     KSGDAKAMLSIGKDGWPFPVPVVKEASGWRFDTRQGKEEILNRRIGRNELSVIQVLQA
                     YVDAQREYYLRNPQGDKLLHYAQRIASTQGKRDGLYFPTKAGERPSPLGPLVESARAE
                     GYKKDASGKPVPYHGYYYRVLRGQGPDAPGGAYDYVAQGRMIGGFAMIAWPATYETSG
                     VMTFMVSHAGVVYERDLGPETA"
     gene            complement(960..2159)
                     /locus_tag="AC158Q_04730"
     CDS             complement(960..2159)
                     /locus_tag="AC158Q_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HIR3339110.1"
                     /translation="MKRIARFMLVWVIAALALAPLGRAQDAPKPFSKEELEQLVAPIA
                     LYPDALVAQILMAATYPLEVVSAARWSKANPNVKDKALEDAMQKQPWEASVKSLTAFP
                     QVLAMMNEKLDMTQKLGDAFLGQQKDVMAAIQRLRAKADKAGNLKSSKEQTVTTVQES
                     GQTVIKIEPADPQVVYVPTYNPAVVYGPWPYPAYPPYYYYPPGYVPGAAFFTFTAGVI
                     IGSALWGGCNWGGGGVYINHNTYNNFNKTNIKSGDWQHRPENRKGVQYRDQASQQKFG
                     GGQRAGVDSREQFRGRAEQGRQDIARDREGVGRDVSTADRARGADRGAGVPDRAGGAG
                     DRAGVGDRSAGVGDRTGGAGDRAGTRQAGGFDGIGSGGQTRSASSRGASSRASAGASR
                     GGGGRRR"
     gene            complement(2215..3531)
                     /locus_tag="AC158Q_04735"
     CDS             complement(2215..3531)
                     /locus_tag="AC158Q_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HIR3339111.1"
                     /translation="MKHALTPVAISIVATVLAVAPVQAAQPAPFSDRDIIQAYHYLLG
                     RLLVIRQEHLDLKEGFKWNQFVHRAPGAISRAGLDPGIAYSEAWVAVDEKSCTIVTLP
                     VIKGRYYTVQLLSGWGETVANISERTRPKHPAGGYAMCLKGADVHLPRDARRIDLPGR
                     KSRVVARVELGADPAEAVRLQKQITMKPTGSPRAEPPVAITVFFNDQLPRVEAFNRVD
                     ALLASEPDSSPGMGPLQARARAIAKAARNVEELKRIDDVIVKRAIPQFMAGLQQIGKT
                     RNGWHRPARTGDYGSDYLSRSMASFVDIRANTSREAVHYTTETDGKGQKLEGSNVYTM
                     TFPKGQPQALARYFWSVVAVDPAAFTVMDNPLKRYLLSNQSALQPNPDGSVTLFFAAK
                     QPVGAPPQNWLPTPAGKPYNLTFRFYGPGDKVISGGYFPPPLVKTI"
     gene            complement(3724..5124)
                     /locus_tag="AC158Q_04740"
     CDS             complement(3724..5124)
                     /locus_tag="AC158Q_04740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HIR3339112.1"
                     /translation="MSRLLLAAILSAAVSACALKSPPEHADLRSDALPNLGIPAQWTA
                     KGGSAGAVAGGWLATFADERLDALVREAIANNPDLRVAAARVEQAAGYARLAGATLYP
                     QVNLMARGGGKLSGDGSGLQGAGIFASWELDLWGRVRAGRESAQLQYVSAELDTEYAR
                     QSIAALVAKSWFLATEARLQKGIAEEMVRSAERLLGLAGDRLRVGKGDEYDAVLARAN
                     LQTYRDVVQQLDLSYQQAVRALETLAGRYPAAAAAVPDRLAPKPGPIPAGMPSGLLER
                     RPDVAAAERRVAAAFYRVEESKAARLPRISLSASGTSISSDLIVLKNHDNPVWGLGAS
                     ILAPIFTGGALQSQVEIRTAEQKVAVAEYGRVGARAFGEVENALSAEFTLDERASILA
                     GAVAENERALELANVRYRVGSADLRAVQQQQLAVYAARTSLLRVRSEQLVQRVNLHLA
                     LGGGWVDESGRSASTQ"
     gene            complement(5135..6316)
                     /locus_tag="AC158Q_04745"
     CDS             complement(5135..6316)
                     /locus_tag="AC158Q_04745"
                     /inference="COORDINATES: protein motif:HMM:NF052759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIR3339113.1"
                     /translation="MEVILLGIYSFFVWLIFIKLKWLPWNIKTQVIVVIIPIVGMAAL
                     ILLLNIFAPSSSDVRVFKYIVPIVSQVRGRVIEVPVEEGNRLVKKGEVLFKIDPEPYR
                     LAVNQLEAQLATAVASQKELQESLKAARGSVEQATARSREVSARLELARVRVAQNREL
                     VATGAGSKFDLEQAETNLKELEGQADAARASQVQAQAGEQQVRKKLGGQVNGEFAQVA
                     QIRAQLENARWELEQTVTLSPCDCYVVNLQLRPGAFVAGMPFNAVMSLVEAQGQVVAL
                     FNQNELHQIKPGNAAEFTLYTDPGRVIRGKVDSIIWAQGQGQLPQSGTLPMTGVLNAP
                     PGRFAVKFDIEDKDMEVFTAAGAAGAAAVYTDRLAAIHIIRMVILRVGAYTNYLILKL
                     H"
     gene            complement(6317..6871)
                     /locus_tag="AC158Q_04750"
     CDS             complement(6317..6871)
                     /locus_tag="AC158Q_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HIR3339114.1"
                     /translation="MLKQVAVGWLMALAASPARAAFLAGEALDTAADILALVVLFLVP
                     AVVIVLFWIVHVLPEKIAHKRHHPQREAITTLCLLSLVFGGLLWPLAWIWAYTKPVAY
                     RVAYGTDKHEDYYDEMAEKQREGKLLREDAYHLREELEAMEARGALPPKLRALKDELA
                     RLRLADAEERADRARAQAKDEAAA"
BASE COUNT         1078 a         2468 c         2162 g         1276 t
ORIGIN      
        1 ggcggtctcc ggcccgaggt ccctttcgta caccaccccg gcgtggctga ccatgaaagt
       61 catgaccccc gaagtctcgt acgtggcagg ccaggcaatc atcgcgaagc cgccgatcat
      121 cctgccctgt gcgacgtagt catacgcgcc acccggcgca tcgggcccct gtccccgcag
      181 cacgcgataa taatagccgt ggtagggcac cggcttgccg cttgcgtcct tcttgtagcc
      241 ttccgcccgg gcactttcca ccagtggccc gagcgggctg ggccgctcgc ccgccttggt
      301 cggaaaatag agtccgtcgc gcttgccctg ggtgctcgca atgcgctgcg cgtaatgaag
      361 gagcttgtcc ccctgaggat tgcgcaggta gtactcgcgc tgcgcgtcca cgtacgcctg
      421 gagcacctgg attaccgaca gttcattgcg gccgatccgg cgattgagga tttcctcctt
      481 gccctgccgc gtgtcgaaac gccagccgga agcctctttc acgactggca ccgggaacgg
      541 ccagccgtcc ttgccgatgc tgagcatggc cttcgcgtcg ccggatttct ccagcttgtt
      601 cgccgcttca aaggctttca cgaagcgctc acgtcccgcc ttgtccgcga ccgcgtcgcc
      661 agaccggacg atgtcgcgcg agcccggccc gagtatgccg agcatcgcct tctcgtcgcc
      721 ggacctgatc gccgccacca gttccttcac ggccgcctcc ggtgtcgcat aagtcttttg
      781 tttcgcgtcg gcagcggtcg cgcttgtcgc gcacgcaagc atccctgcca gggcgatcgc
      841 ggcaatggcg ccgcgctgga caatcctgct gcaattgaac tggatcgaaa tcatttcgag
      901 ctccttcgac cggataactg ttatcggcgc gtatcggcgt acatcggcgg ctgactcaac
      961 tacctgcgcc gcccgccgcc gccgcggctg gcgcccgcgc tggcccggct cgatgcgccg
     1021 cgactgctgg cactgcgcgt ttgtccgccg ctgccgatgc cgtcgaaacc gccggcctgc
     1081 cgtgtgccgg cgcgatcgcc ggccccgccc gttcgatccc cgacccctgc cgagcgatct
     1141 ccgacgcccg cacgatcccc cgcgcctccc gcgcgatcgg ggacaccggc tccccgatcg
     1201 gcgccccgcg cacggtcggc agtcgaaaca tcccggccga ccccttcgcg gtcgcgcgca
     1261 atgtcctggc gtccttgctc cgcgcggccg cggaactgtt cgcgagagtc cacgcccgcg
     1321 cgctggccac cgccgaattt ctgctggctc gcctggtcgc ggtactgcac gcctttccgg
     1381 ttctccggcc tgtgctgcca gtcgccgctc ttgatgttgg tcttgttgaa gttgttgtag
     1441 gtgttgtggt tgatgtagac gccaccaccc ccccagttgc agccgcccca cagggccgag
     1501 ccgatgatga cccctgccgt gaacgtgaaa aaggcggcgc ctggaacgta gcccggcggg
     1561 tagtagtagt atggcggata cgccggatac ggccacggcc cgtagaccac tgccgggttg
     1621 taggtcggca cgtaaaccac ctgcggatcc gccggctcga tcttgatcac cgtttgcccg
     1681 ctctcctgca cggtggtgac ggtctgctcc ttgctcgact tcaggttgcc cgccttgtcc
     1741 gctttggcgc gcaaccgctg gatcgcggcc atcacgtcct tctgctggcc gagaaaggcg
     1801 tcgccgagct tctgggtcat gtccagcttt tcgttcatca tcgcgagaac ctgggggaaa
     1861 gcggtgagcg acttcacgct ggcctcccac ggctgcttct gcatcgcgtc ttcgagcgcc
     1921 ttgtccttga cgttcgggtt ggctttcgac cagcgcgccg ccgacacgac ctcgagcggg
     1981 tacgtggccg ccatgagtat ctgcgccacc agcgcatcgg gatagagagc gatcggcgcg
     2041 acgagctgct cgagttcttc cttcgagaac ggcttgggcg cgtcctgcgc ccggcccaac
     2101 ggcgctaacg ccagcgccgc gatcacccac accagcatga atctcgcaat ccgtttcatc
     2161 tcaccccccc tcgcgaatcg ccgcgtcact tcagcactgt atcaccgttt catgtcagat
     2221 cgtcttcacc agcggtggcg ggaagtagcc gcccgagatt actttatcgc cgggcccgta
     2281 gaagcggaaa gtgagattat agggtttccc cgccggcgtc ggcagccaat tctgcggcgg
     2341 cgcgccgacg ggttgcttcg cggcaaaaaa gagcgtgacg gagccgtccg ggttgggctg
     2401 cagcgcggac tggttgctga gtaggtaacg cttgagcgga ttgtccatta ccgtgaacgc
     2461 ggccggatcc accgcgacca ccgaccagaa gtagcgggcc agcgcctgcg gctgcccctt
     2521 cgggaaggtc atcgtgtaga cattcgagcc ctcgagcttc tgtcccttgc cgtcggtctc
     2581 cgtggtgtaa tggacggcct cccggctggt gttcgcccgg atgtcgacga agctggccat
     2641 gctgcgcgac aggtaatcgc tgccgtagtc gccggtcctc gccggccggt gccagccgtt
     2701 gcgcgtcttt ccgatttgct gcagtcccgc catgaactgc gggatcgccc gcttgacgat
     2761 gacgtcgtca atgcgtttca actcctcgac gttgcgggcg gctttcgcga tcgcgcgcgc
     2821 cctcgcctgc agcggcccca taccggggct gctgtcgggt tcgctcgcca gcagcgcatc
     2881 cacccggttg aacgcttcaa cgcgcggcaa ctggtcgttg aagaacaccg tgatcgccac
     2941 cggcggctcg gccctgggcg agccggtcgg cttcatcgtg atctgctttt gcagccgcac
     3001 cgcctccgcc gggtcggcgc caagctcgac gcgcgcgacc acgcgggatt tcctgccggg
     3061 cagatcgatt cgccgcgcgt ccctgggcag gtggacatcg gcgcccttca ggcacatcgc
     3121 ataaccaccg gcgggatgtt tcggccgggt gcgctcgctg atgttggcga cggtctcgcc
     3181 ccacccgctc agcaactgca cggtgtagta ccgccccttg atcaccggca acgtgactat
     3241 cgtgcagctt ttttcgtcca ccgcgaccca ggcttcgctg taagcgatgc cgggatccag
     3301 gccggcccgg gagatggcgc ctggcgcccg gtggacgaac tggttccact tgaacccttc
     3361 cttcaggtcg agatgctcct gccggatgac cagcaggcgg cccagcagat agtggtaagc
     3421 ctggatgatg tccctgtcgc tgaacggcgc gggctgcgct gcctggacag gcgccacggc
     3481 cagcacggtc gcgacgatcg agatagcgac gggagtcagg gcgtgtttca tgattgcaat
     3541 cctcgttcca cagcacgcat cccgagccgg gatgcaaaaa cccctgtggc gccggattta
     3601 ttattgaatg cagcgcttgc ggactggcaa ccgcacgcgc ctcatgcggg tgactgggaa
     3661 gcatccggcc tcatgccccc cgccaccgcg gtcaacaccc ctacgttacc ggaccggaat
     3721 ttgtcattga gtgctcgcgc ttcttccgga ctcgtccacc cagccgccgc caagcgcgag
     3781 atggagattc acgcgctgca ccagctgctc gctccgcacg cgaagcagcg aggtgcgcgc
     3841 ggcatacact gccaattgct gttgctggac ggctcgcagg tcagccgatc ccacgcggta
     3901 gcgcacgttc gcgagttcga gcgcgcgttc gttctccgcg acggcaccgg caaggatgga
     3961 ggcccgctcg tcgagcgtga attccgctga caaggcgttt tccacttccc cgaacgcccg
     4021 tgcccccacg cggccgtatt cggccaccgc cactttctgt tccgcggtac ggatctcgac
     4081 ctgcgactgc aaggcgccac cggtgaaaat cggtgcgagt atgctcgccc ccaggcccca
     4141 taccggattg tcgtgattct tcagcacgat caaatcgctc gatatgctgg tgccgctcgc
     4201 gctcagcgag atccgcggca gacgcgccgc cttggactcc tcgacccggt agaacgcggc
     4261 cgcgacgcgc cgctccgccg ccgccacgtc gggccggcgc tcgagcagcc cggaaggcat
     4321 gccggccggt atcggccccg gcttgggtgc aagccggtca ggaactgcag ccgccgcggc
     4381 gggataccgc ccggccagag tttcaagcgc gcgcacggcc tgctggtacg agagatcgag
     4441 ctgctgcacc acgtcccggt aggtctgcag gttcgcgcgc gcaagcaccg catcgtattc
     4501 gtcgcctttg ccgacgcgca gccggtctcc cgcgagaccc agcagccgct cggcggagcg
     4561 caccatttcc tccgcgatgc ccttctgaag ccgcgcctcg gtggcgagga accagctctt
     4621 ggcgaccagc gcagcgatgg attgccgcgc gtactcggta tcgagctccg cagagacgta
     4681 ctggagctgg gccgattcgc gccctgcgcg cacgcgcccc caaaggtcga gttcccagct
     4741 ggcgaatata ccagcgccct gcaagccgct gccatcgccg ctcagcttgc ccccgccacg
     4801 cgccatgagg ttgacctgcg ggtagagcgt cgcgcccgcc agcctggcgt agcctgccgc
     4861 ctgctcgacc cgcgcggcag caacacgcag gtcggggtta ttggcgatgg cttcgcgcac
     4921 cagcgcatcg agccgctcat ccgcgaacgt cgcgagccag cctccggcta cggcgcccgc
     4981 ggatcctccc ttggcagtcc attgcgccgg aatccccagg ttgggcagcg catcgctgcg
     5041 caggtccgcg tgctccggcg gcgacttcag cgcgcaggcg gaaacggcgg cagacaggat
     5101 ggcggcgagc aacagtcggc tcacgttcgc gttctcagtg cagcttgagg atcaggtagt
     5161 tggtgtaggc gcctacgcgc aggatcacca tgcggatgat gtgtatcgcc gcaagccgat
     5221 cggtgtacac cgccgccgcg ccagcggcgc ccgcggccgt aaatacttcc atgtccttgt
     5281 cctcaatgtc gaatttgacg gcaaagcggc ccggcggggc attcagcacc ccggtcatcg
     5341 gcagcgtacc gctctgcggc agctggccct gtccttgcgc ccagatgatc gagtcgacct
     5401 tgccccgaat gacccgcccc gggtcggtgt agagcgtgaa ctcggccgcg tttccgggct
     5461 tgatctggtg cagttcgttc tggttgaaaa gcgcgaccac ctgcccctgc gcttcgacca
     5521 gcgacatcac cgcgttgaaa ggcatgcctg cgacgaacgc gcccggccgc agctggagat
     5581 tcaccacgta gcaatcgcag ggagacagcg tcacggtctg ctccagctcc cagcgcgcgt
     5641 tctcgagttg agcccggatc tgggccacct gcgcaaactc gccgttcacc tgcccgccca
     5701 gcttcttccg gacctgctgt tcgcccgcct gcgcctggac ctgcgacgcg cgcgccgcgt
     5761 ccgcctggcc ttccagttcc ttgagattgg tctcggcctg ctcgaggtcg aacttgctgc
     5821 cggcgcccgt cgcgaccagt tcgcggttct gcgccacacg cacgcgcgcg agctccagcc
     5881 gcgcgctcac ctcgcgcgag cgggccgtcg cctgctcgac cgagccgcgc gctgccttca
     5941 acgattcctg cagctccttc tgcgacgcca cggcggtggc gagctgcgcc tcgagctggt
     6001 tgacggccag gcgatagggc tccgggtcga tcttgaacag cacctcgcct ttcttcacca
     6061 gccggttgcc ttcctcgacc ggtacctcga tcacgcgccc gcgcacctgt gacacgatcg
     6121 gcacgatgta cttgaacacg cgcacgtcgg aggacgaagg cgcgaagatg ttgagcagca
     6181 ggatcagcgc tgccatcccg acgatcggga tgatgacgac gatgacctgc gttttgatgt
     6241 tccacggcag ccacttcagc ttgatgaaga tcagccagac gaagaacgaa tagatcccga
     6301 ggaggatgac ttccatctac gccgccgctt cgtccttggc ttgcgcccgc gcgcggtccg
     6361 cgcgctcctc ggcgtcggcg agccgcaggc gggcgagctc gtccttcagc gccctcagct
     6421 tcggcggcag ggcgccgcgc gcctccattg cctccagctc ctcgcgcaga tggtaggcgt
     6481 cttcgcgcag cagcttgccc tcgcgctgct tttccgccat ctcgtcgtag tagtcctcgt
     6541 gcttgtcggt tccgtaggcc acgcggtagg ccacgggctt ggtataggcc cagatccagg
     6601 cgagcggcca gagcaggccg ccgaacacga gcgacagcag gcatagcgtg gtgatcgctt
     6661 cgcgctgcgg atggtgacgc ttgtgggcga tcttctccgg cagcacgtgg acgatccaga
     6721 acagcacgat caccaccgcc ggaacgagaa acagcacgac cagcgcgagg atatcggccg
     6781 cagtgtcgag cgcctcgccc gccaggaacg cggcgcgcgc cggcgaggcg gcgagcgcca
     6841 tcagccagcc gacagcaact tgcttcagca tgaccccatc ctgccttcct gtttcgagcg
     6901 ccggaatccc accaaggtac gattttagcg aggcatttcc ggtggcctaa accatcggcc
     6961 tcgcccgccg cgagaatccc tcct
//
LOCUS       DBNGSG010000184         6615 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_741513, whole genome shotgun sequence.
ACCESSION   DBNGSG010000184 DBNGSG010000000
VERSION     DBNGSG010000184.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6615)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6615)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6615
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_741513"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..1332)
                     /locus_tag="AC158Q_04755"
     CDS             complement(130..1332)
                     /locus_tag="AC158Q_04755"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HIR3339115.1"
                     /translation="MKKRTLGRYEILAELGKGAMGVVYRANDPQLSRTVAIKTVNMSE
                     DPQEMAEYEARFNQEAKAAGGLNHPNIVTIYDIGKSGNIAYMTMELLEGSELRALMSQ
                     GAPIGMTRAVEIAAQVAEGLAYAHQHGVVHRDVKPANIMILNSGPAKITDFGIARMRQ
                     AEVRTQTGIVLGSPRYMSPEQVAGKRAEPRSDIFSLGVIFYEMLTGKPAFTGDDVTAV
                     MFQILNVVPPPPSSINPAIPAVLDFIVAKALAKNAEDRYQDAGEFAHDLRDSAKQVAE
                     IAQAPAIASTVPQAALDSEATQALARSLPGEPLDDAVGTVSDPAPVLGLSKAFNSLDA
                     TMKLAAQTGMMESIKELSEQADGSATEARLMVGEMRKGPGWSKRDKTIFAASVAGATI
                     VGAIIIFV"
     gene            complement(1337..1747)
                     /locus_tag="AC158Q_04760"
     CDS             complement(1337..1747)
                     /locus_tag="AC158Q_04760"
                     /inference="COORDINATES: protein motif:HMM:NF038027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TssQ family T6SS-associated lipoprotein"
                     /protein_id="HIR3339116.1"
                     /translation="MKILRLMLLTALVAALAGCESAPIKGIKEGFSNLFQTAGNAGAQ
                     DLANGIKQYEDGKYAPASRLLQSSLDKGLSGTSDQVRAHKYLAFIHCVSGREARCREE
                     FLAALEIDPAFELAAAESGHPIWGPVFRSVKGGR"
     gene            complement(1762..2889)
                     /locus_tag="AC158Q_04765"
     CDS             complement(1762..2889)
                     /locus_tag="AC158Q_04765"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR3339117.1"
                     /translation="MQASNRTFICSVVFLDIVGYSKKPVSEQIQLKERLNTLLTEALE
                     NVAVNDRIILDTGDGAALSFLGDPEDALFVSLMLRDAVAAPAPSGPDLAMRIGINLGP
                     VKVVKDINGQPNIIGDGINVAQRVMSFSKSGQVLVSRSYYEVVSRMSEEYVRLFHYEG
                     SRTDKHVREHEVYAVQATGEALKHVAAAAPRKKDDTPGAPAVLDKLSQSASFVAYNLR
                     RKPRLGTALAVVAILAVAVGLRLNRQPLAEPVAKPAPPTKIATAPAPAPVKETPKVSL
                     PDKPVATAPKAAPAPEKPAPARKPDAASPSGPGVVGFTVLPWGEVYINGRRHGVAPPL
                     REVELNPGQYKVEIRNAGFEPYVEVVDVKPGSRIRIRHRFR"
     gene            complement(2977..5031)
                     /gene="metG"
                     /locus_tag="AC158Q_04770"
     CDS             complement(2977..5031)
                     /gene="metG"
                     /locus_tag="AC158Q_04770"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HIR3339118.1"
                     /translation="MTQRRIFVTSALPYANGAIHVGHLVGYIQADIWVRFQRMRDNVV
                     HYCCADDTHGTPIMLRAEQEGITPEALIARVLEEHKRDFKGFHVEFDNYHTTHSEETR
                     KLSEEIYARISAANLIEVRPVEQFFDPVKQMFLPDRFIKGECPKCGTKDQYGDSCENC
                     GATYSPTDLKNPYSTVSGAKPVRKSSDHHFFRLSDPKCREFLEKWTQEDEHLQPEGRN
                     KIREWFDAGLNDWDISRDAPYFGFEIPGAPGKYFYVWLDAPVGYFGSFKNLADRSGSK
                     IRFEDFVGKESKTEMIHFIGKDILYFHALFWPAMLRFSGYRTPSHLYVNGFLTVDGVK
                     MSKSRGTFITAESYLRQGLNPEWLRYYYAAKLGPTMEDIDLSLEDFVARVNSDLVGKY
                     VNIASRASTFLSKYFNGEVAGSSKSDKEHFSALWTVSIEDEVRDAYNARDYGKAIRSI
                     MAFADQANQYFDANKPWDLAKNREDHTRLHRVCSEAISAFYRITVFLKPVLPELVKQA
                     ERFLNTPNLSWKDLGGRPKRIGKYEHLMTRVDPKQIAALVEANKENLQVTTPTTTPTP
                     APAPAAQPGGGTISIDDFSKIDLRIARIANAEAIEGADKLLKLTLDLGGTTKTVFAGI
                     KSAYDPEKLKGRLTVVVANLAPRKMKFGVSEGMVLAASGDGPGIFILSPDAGAEPGMK
                     VK"
     gene            5152..6240
                     /gene="apbC"
                     /locus_tag="AC158Q_04775"
     CDS             5152..6240
                     /gene="apbC"
                     /locus_tag="AC158Q_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HIR3339119.1"
                     /translation="MAVTEQQVQDALKGLTDPNTQKDYVTTKSVRNVKVDGEKVSLEI
                     LLGYPGKSQVEPIREQVTKALKALPGVSGVSVNVQMKIISHAVQRGVKLVPNIKNIIA
                     VASGKGGVGKSTTAVNLALALAAEGASVGVLDADIYGPSQPIMLGIKGRPESKDGKSI
                     EPMDGHGVQAMSIGFLIDPDTPMVWRGPMVTQALEQLLNETKWREIDYLVVDLPPGTG
                     DIQLTLAQRVPVTGAVIVTTPQDIALIDARKGLKMFEKVGIPILGIVENMSLHVCSKC
                     GHEERIFGEGGGERMGKDYNIELLGALPLDIQIREQTDSGRPTVVADPDGRASQIYKA
                     IARRVAVKIAEKAQDHTAAFPKIVIQNT"
     gene            6442..>6615
                     /gene="dcd"
                     /locus_tag="AC158Q_04780"
     CDS             6442..>6615
                     /gene="dcd"
                     /locus_tag="AC158Q_04780"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Catalyzes the formation of dUTP from dCTP in
                     thymidylate biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HIR3339120.1"
                     /translation="MSIKSDRWIRRMAREARMIEPFEANQIKEVKGRKVVSFGTSSYG
                     YDIRCSDEFKLFTN"
BASE COUNT         1186 a         2006 c         2099 g         1324 t
ORIGIN      
        1 tcgcttcgtt gctctctatt ttcatgtgcg taagtggtga ttggtgattg gtgatcggac
       61 ttgcctgtcc acaaagccaa cgctgagggc tccggtcgat tcaatactca ccattcactc
      121 ttcacgcact cagacaaaaa tgatgatggc gccgacaatc gttgcgccgg cgacacttgc
      181 ggcgaagatg gttttgtccc tcttgctcca gcccggtccc ttgcgcatct ctccgaccat
      241 caggcgtgct tcggtggcgg agccatccgc ctgttcggac agctccttta tcgactccat
      301 catcccggtc tgggccgcca gcttcatagt cgcatcgagg gagttgaagg ccttggacag
      361 gcccagtacc ggcgcagggt cggagaccgt gccgaccgca tcgtcgagcg gctcgcccgg
      421 cagcgatcgc gccaacgcct gggtcgcttc cgaatcgagc gcagcctgcg ggaccgtgct
      481 ggctattgcg ggcgcctggg cgatctcggc cacttgcttc gcgctatcac gcaggtcgtg
      541 ggcgaattcg ccggcgtcct gataacggtc ctcggcgttc ttggcgagcg ccttggcgac
      601 gatgaaatcc agcaccgcgg gaatggccgg gttgatcgaa ctcggcggcg gcggcaccac
      661 gttgaggatc tggaacatga ccgcggtcac gtcatctccg gtgaacgccg gtttgccggt
      721 cagcatttcg tagaaaatca cgcccaggga gaagatgtcg gaacgcggct cggcgcgctt
      781 gcccgcgacc tgctcgggcg acatgtagcg cggtgagccg agcacgatac cggtctgggt
      841 ccgcacttcg gcctggcgca tgcgcgcgat gccgaagtcg gtgattttcg ccggaccgct
      901 gttgaggatc atgatgttcg cgggtttcac gtcgcggtgc acgacgccat gttggtgcgc
      961 gtaggcgagc ccttccgcga cctgggcagc gatctcgacc gcgcgcgtca tcccgattgg
     1021 cgctccctgc gacatcagcg cgcgcaactc gctgccctcg agcagttcca tcgtcatgta
     1081 ggcgatattg ccgctcttgc ctatgtcgta gatggtgacg atgttgggat ggttgagccc
     1141 gcccgcggcc ttggcttcct ggttgaagcg cgcctcgtac tccgccatct cctgcgggtc
     1201 ctccgacatg ttgacggtct tgatcgcgac cgtccggctg agctgcgggt cgttcgcccg
     1261 gtagacgaca cccatcgcgc ccttgccgag ttcggcgagg atctcgtagc gaccgagggt
     1321 gcgtttcttc atgaattcag cggccgccct tgacgctgcg gaacaccggc ccccagatcg
     1381 ggtggccgga ttcagccgcc gcgagctcga aggcggggtc gatctcgagc gccgcgagga
     1441 attcctcccg gcagcgcgct tcgcggccgg agacacagtg gatgaaggcg agatacttgt
     1501 gtgccctgac ctggtcggaa gtgccggaga gccccttgtc gagactggat tgcagcaggc
     1561 gcgaggccgg ggcgtacttg ccgtcctcgt attgcttgat tccgttcgca aggtcctgcg
     1621 cccccgcgtt gccggccgtc tggaataggt tgctgaagcc ttccttgatt cccttgatgg
     1681 gagccgactc gcagcccgcc aaggctgcca caagggccgt cagcagcatt aaacgcaata
     1741 ttttcatggt gttcctcccc cttaccgaaa ccggtgccta attctaatcc ggctgccggg
     1801 ctttacgtcg accacttcca cataaggctc gaaaccggcg tttctgatct cgaccttgta
     1861 ttggcctgga ttgagttcga cctcgcgtaa cggaggggcg acaccgtgac gcctgccgtt
     1921 gatataaacc tcgccccagg gcagaaccgt gaagccgacc acgcccggtc ccgatggcga
     1981 ggcggcatcc ggctttctgg ccggagcggg cttctcggga gctggcgcgg ccttgggagc
     2041 ggtggccacg ggtttgtcgg gcaaggagac cttgggggtt tcctttacgg gggcgggggc
     2101 cggcgccgta gcgattttcg tcggcggtgc tggttttgcc accggctccg cgagcggctg
     2161 gcggttcaag cgcaacccca cggcgacagc gaggatggcg accacggcga gcgcggtgcc
     2221 gagccggggc ttgcgccgaa ggttataggc gacgaacgaa gcggattgcg acagcttgtc
     2281 gagcacggcc ggtgcgcccg gggtgtcatc cttcttccgg ggtgccgcgg cggcaacgtg
     2341 cttcaatgcc tcgccggtcg cctgcacggc ataaacctcg tgctcgcgca cgtgcttgtc
     2401 ggtgcgcgag ccctcgtagt ggaacagccg gacatattcc tcggacatgc gggacaccac
     2461 ctcgtagtag gagcgcgata cgaggacctg gcccgacttc gagaagctca tcacccgctg
     2521 cgcgacgttg atgccatcgc cgatgatgtt gggttggcca ttgatgtcct tgaccacctt
     2581 gaccgggccg agattgatgc cgatgcgcat cgccagatcc gggccggatg gcgccggagc
     2641 ggcgacagcg tcgcgcagca tgaggctcac gaacagcgca tcctccgggt cgccgaggaa
     2701 ggatagcgcc gcgccgtcac ccgtatcgag gatgatgcgg tcgttcaccg cgacgttctc
     2761 cagcgcctcg gtgagcaggg tgttcagccg ttccttcagc tggatctgct cggagacggg
     2821 cttcttcgag tacccaacga tgtcgaggaa cacgacgctg cagatgaaag tgcggttgct
     2881 ggcttgcatc gccttggctc aggaatcgta agtcgtgatt cgtgattcga gattcgaccg
     2941 ggcacgcatg gttcttgtcc tcttgcgcgc tgtgcgctac ttcaccttca tccccggctc
     3001 ggcgccggcg tccgggctca ggatgaagat gccggggccg tcgccggagg cggcgaggac
     3061 catgccctcg gagacgccga atttcatctt gcgcggcgcg agattggcga ccacgaccgt
     3121 cagcctcccc ttgagctttt cgggatcgta agccgatttg atcccggcga acaccgtttt
     3181 cgtcgtgccg ccgaggtcga gcgtgagctt cagcagctta tcggcgccct cgatggcctc
     3241 cgcattggcg attctggcga tccggaggtc gattttgctg aaatcgtcga ttgagatcgt
     3301 gccgccgccg ggttgcgctg ccggggcggg tgccggggtc ggggtggttg ttggtgtagt
     3361 cacttgtagg ttttccttgt tggcttctac cagggcggca atttgcttcg gatcgacacg
     3421 ggtcataagg tgctcgtatt ttccaattcg ctttgggcgg ccgcccaggt ctttccagga
     3481 aagattcggc gtatttagga agcgctccgc ctgctttacc aattctggta aaaccggctt
     3541 cagaaataca gtgatacggt aaaaagcaga gattgcttcg ctgcataccc ggtgcaatct
     3601 tgtgtgatct tccctgttct tggccaaatc ccaaggttta tttgcgtcaa agtactgatt
     3661 tgcctgatcg gcaaaagcca tgatggatct gatcgcttta ccgtaatcgc gggcgttata
     3721 cgcgtcccgc acttcatctt cgatgcttac ggtccacaag gccgagaaat gctctttgtc
     3781 tgacttagaa gatccggcca cctctccatt gaaatatttc gacaagaagg tcgaagcgcg
     3841 gcttgcgata ttcacgtatt ttccgacgag gtcgctgttc acccgcgcga cgaaatcctc
     3901 taggctgaga tcgatgtctt ccatcgtcgg cccgagcttg gcggcgtagt agtagcgcag
     3961 ccactcgggg ttgaggccct gcctgaggta gctctccgcg gtgataaagg tgccgcgcga
     4021 cttggacatc ttcacgccgt ccaccgtcag aaagccgttc acgtaaagat gtgacggcgt
     4081 gcggtaaccc gagaacctga gcatagccgg ccagaacagg gcgtgaaagt agaggatgtc
     4141 cttgccgatg aaatggatca tctcggtttt cgattccttg cccacgaagt cttcaaatct
     4201 gatcttgctc ccgctgcggt cggcgaggtt cttgaagctg ccgaagtaac cgaccggggc
     4261 gtcgagccag acgtagaagt acttgccggg ggctcccgga atctcgaagc cgaagtaggg
     4321 tgcgtcacgg gagatgtccc agtcgttgag accggcgtcg aaccactccc ggatcttgtt
     4381 ccggccctcc ggttgcaggt gttcgtcctc ctgtgtccat ttctccagaa actcgcggca
     4441 tttcgggtcg gacagcctga agaaatggtg gtcggatgac ttgcgcaccg gcttggcgcc
     4501 cgacacggtc gagtaggggt tcttgaggtc ggtcgggctg taagtcgcgc cgcagttctc
     4561 gcacgagtcg ccgtactggt ctttcgtgcc gcacttcggg cactcgccct tgatgaagcg
     4621 gtccggcagg aacatctgct tcaccgggtc gaagaattgc tcgaccggcc tgacctcgat
     4681 caggttcgcc gcactgatcc tggcgtagat ttcctccgac agcttgcgcg tttcctcgga
     4741 atgcgtggtg tggtagttgt cgaactcgac atggaagccc ttgaaatcgc gcttgtgttc
     4801 ttccagcact cgcgcgatca gggcctccgg cgtgatgccc tcctgctcgg cgcgcagcat
     4861 gatcggggtg ccgtgagtgt cgtccgcgca gcaataatgg acgacattgt cccgcatgcg
     4921 ctggaagcgc acccagatat cggcctggat gtatcccacc agatggccga cgtggatcgc
     4981 cccgttggcg taggggagag ccgaagtcac gaagatgcgg cgctgggtca tgggcagggt
     5041 gaacttaagg ccctgaattg taacaaacag tccatgcggg ctcgcctatt agtcgcgctg
     5101 tccgcaatgg tctagaatcg cgccctgaac gcggaaagag gcaactacct aatggcagtc
     5161 accgagcaac aggtccagga cgctctcaag ggcctgacag accccaacac ccagaaggac
     5221 tacgtcacca ccaagtcggt ccgcaacgtc aaggtggacg gcgaaaaggt gtcgctggag
     5281 atcctgctgg gctaccccgg aaagagccag gtcgaaccga tccgggagca ggtgacgaag
     5341 gcgctgaagg cgctgccggg cgtcagtggc gtcagcgtca acgtgcagat gaagatcatc
     5401 tcgcatgccg tccagcgcgg cgtgaagctg gtgcccaaca tcaagaacat catcgccgtc
     5461 gcctccggca agggcggggt gggcaagagc acgaccgctg taaacctcgc gctcgccctc
     5521 gcggcggaag gcgccagcgt gggggtgctc gatgccgaca tctatggccc gtcgcagccg
     5581 atcatgctcg gcatcaaggg gcgccccgaa tcgaaggacg gcaagagcat cgagcccatg
     5641 gacggccacg gtgtccaggc catgtccatc ggtttcctga tcgacccgga caccccgatg
     5701 gtgtggcgcg gcccgatggt gacccaggcg ctggaacagc tcctgaatga aaccaagtgg
     5761 cgggagatcg actacctggt ggtggacctg ccgccgggga ccggcgacat ccagctgacg
     5821 ctcgctcagc gcgtgccggt gaccggcgcc gtcatcgtca ccaccccgca ggacatcgcg
     5881 ctgatcgacg cccgcaaggg actcaagatg ttcgagaagg tcggcatccc gatcctgggc
     5941 atcgtcgaga acatgagcct tcacgtttgc tcgaaatgcg gccacgaaga gcgcatcttc
     6001 ggcgaggggg gcggggagcg catgggcaag gactacaaca tcgagctgct gggcgcgttg
     6061 ccgctcgaca tccaaatccg cgagcagacc gactccggca ggccgacggt ggtagccgac
     6121 cccgacggcc gggcgagcca gatctacaag gcaatcgcgc gccgcgtggc ggtcaagatc
     6181 gccgagaagg cccaggacca cacggcggcg tttccgaaga tcgtcatcca gaacacctga
     6241 ccgggacgga cgcttgcgcg ccgccgcggt ctgctcgcgt tcgaacggac gggccggcac
     6301 cgttcaacgc tcgcctcagc ccatcatttc tgacagtcag ggggagttag ggcatgagac
     6361 gaggtgtaga atcctgacgc ccggtagtct tgcggccagg gctacccatc actcatcacc
     6421 cattactcgt caccgcgaag catgagcatc aaatccgacc ggtggattcg ccgcatggcg
     6481 cgcgaagccc gcatgatcga gccgttcgag gccaaccaga tcaaggaagt gaagggccgc
     6541 aaggtggtgt cgttcggcac ctcgagctac ggctacgaca tccgctgttc cgacgaattc
     6601 aagctcttca ccaac
//
LOCUS       DBNGSG010000185        17054 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_741760, whole genome shotgun sequence.
ACCESSION   DBNGSG010000185 DBNGSG010000000
VERSION     DBNGSG010000185.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 17054)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17054)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17054
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_741760"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="AC158Q_04785"
     CDS             <1..356
                     /locus_tag="AC158Q_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR3339121.1"
                     /translation="EPSSLLCVFVIAAASAEEAERLSASIALRRLNMDYGVNAPVPNH
                     EEARNYPYTEADRRRIVHNRRRLVLGTPGVVRDRLLELQTRFEADELMVITITGDYDS
                     RLQSYELLAGAFNLG"
     gene            376..1128
                     /locus_tag="AC158Q_04790"
     CDS             376..1128
                     /locus_tag="AC158Q_04790"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HIR3339122.1"
                     /translation="MKVIRDCLAALIGALIPATGGVVQAQGQDAYEAPRKRMMEEISA
                     LVRETRLETGRAALSGRVMAAMAKVPRHEFVPDGEKRNAYANRPLPIGLGQTISQPYI
                     VALMTDLMEVKPGDRVLEIGTGSGYQAAVLAELAETVYTVEIVEPLAREARERLKGYR
                     NVITRAGDGYQGWPEHAPFDAIMVTAAPREVPQPLIDQLKPGGRLVVPVGGQATGQSL
                     LLIEKRPDGTVTRRNVLAVRFVPLTDKSGKQQ"
     gene            complement(1198..1968)
                     /locus_tag="AC158Q_04795"
     CDS             complement(1198..1968)
                     /locus_tag="AC158Q_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339123.1"
                     /translation="MRYPYNLALIALFAFAPGAAMGQMSPERVAGLGLRSQADMAPVS
                     ADLAFGSEAAQVWQTMRSLDYSLYGGARAAPNFGLRAAESYGGIVYSLPGGWGSSLEA
                     AYIQGSPASPRRYALTGQLHASFSEGRTLSVGLKYRVRDADSGPGYGMAGDMPTGNGY
                     TLLPSRLSGAGLAPSYQLQMSYQHSASSTFGLALGRDVETFTPFLDTIGPGQRQVMFT
                     GQHWLTPSWALSYDLQPQDAASPLRLQGLLRLGVRYRF"
     gene            2254..3237
                     /gene="ybiB"
                     /locus_tag="AC158Q_04800"
     CDS             2254..3237
                     /gene="ybiB"
                     /locus_tag="AC158Q_04800"
                     /inference="COORDINATES: protein motif:HMM:NF006005.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HIR3339124.1"
                     /translation="MSLTQAIKLLSGEMAGERELSEREAFALMSAMLDGGMGELELGA
                     LLALLEHKPVTLCELLGYCAALAPRCSRLKPPSAQSRPVVFAAYHGVRSQPHLLPLVA
                     LTLQRLGVPVLVHGALDGAGGVAAAYVFRELGVLPCATLAQAQSRLDERKLTFVPTAV
                     LAPGFAELLALKGRLGFGSFIQALARLLMPFEAEALYVVGVNPVTGGALLREYLAISG
                     ARALLLEGVEGEAFADPRRRPQIEYVATGDFRVLFDAETVMLKHSATLPFAVDAQTVA
                     AWIRRAMAGEVPLPLPLVNQLACCLYGAGYTDDMNQAKAIVAVETGSLAAA"
     gene            3383..3970
                     /locus_tag="AC158Q_04805"
     CDS             3383..3970
                     /locus_tag="AC158Q_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HIR3339125.1"
                     /translation="MGAIFQSLGKTITAGVVILIVIILASGGAGAKAGDHAWWAFVFR
                     WLHVMSGVMWIGLLWYFKFVQTPSMPKIPDEQKPAVSKVIAPTALFWFRWSALATVVT
                     GLIVGGMNGYIVPALMLTKGVHAIGIGMWLGLIMAFNVWFIIWPNQKKALGIVQVSAE
                     EKAAAAKLAGMTSRINTMLSIPMLFCMVAQQNAGL"
     gene            complement(4058..4519)
                     /locus_tag="AC158Q_04810"
     CDS             complement(4058..4519)
                     /locus_tag="AC158Q_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008326860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HIR3339126.1"
                     /translation="MNSISLRSVRCSLAAAALALAAPAAADAQLPEIVLTVNGHKLTA
                     EVAHTGSSRTQGLMHRRMLPESRGMLFVFDNVALHAMWMMNTYIPLSVAFLDEGGVII
                     NIEDMKPHTQNSHPAAKPAKYALEVNQGWFRKRGIKPGARIEGIERAPPAR"
     gene            complement(4675..5058)
                     /locus_tag="AC158Q_04815"
     CDS             complement(4675..5058)
                     /locus_tag="AC158Q_04815"
                     /EC_number="4.99.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HIR3339127.1"
                     /translation="MADAKKSALVLFAHGARDAQWAEPFRALQKAVSARRPDLTVELA
                     FLEIMEPALCDCVARLAGAGHRRIVIAPLFLAQGGHLRKDLPRLLKDLDNRHPGADIS
                     VLPPIGEVTELLNAIAEWLIDSAPR"
     gene            complement(5206..5652)
                     /gene="dut"
                     /locus_tag="AC158Q_04820"
     CDS             complement(5206..5652)
                     /gene="dut"
                     /locus_tag="AC158Q_04820"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HIR3339128.1"
                     /translation="MKKIDVRILDERLRGQLPQYATPGSAGLDLRACLDTPVTLAPGQ
                     AELIPSGIAIHLADPGLAAVVLPRSGLGHKHGIVLGNLVGLIDSDYQGQVFVSTWNRG
                     NAPFTVNPMERLAQLVVVPVVQVTFNVVEEFGASRRGAGGFGSTGR"
     gene            complement(5712..6917)
                     /gene="coaBC"
                     /locus_tag="AC158Q_04825"
     CDS             complement(5712..6917)
                     /gene="coaBC"
                     /locus_tag="AC158Q_04825"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019256392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HIR3339129.1"
                     /translation="MAERSGKSVVLGVTGGIAAYKAAQLTRDLQRHGIDVQVVMTEAA
                     RGMVTPATFQALSGKPVYTDMWDAGVPDNMAHIELSRGKDAIVIAPATADFIAKLASG
                     LADDLLSTLCLARECPLIVAPAMNRQMWQNPATRRNVDRLLRDGVAILGPASGDQACG
                     EVGMGRMLEAHEIAESVAGFLAPRLLAGARVLITAGPTFEPIDAVRGITNRSSGKMGY
                     AVARAALDAGARVTLVSGPVSLPAPAGAEMSRVGTAAEMFDAVKRHAARADIYISVAA
                     VGDYRVEKPSRHKIKKTEGKRLDLKLVPNPDILAWVAARPRPPFCVGFAAESRNLDAY
                     ADEKRRRKKVPLMVANLAQEAIGSDENEVTLFDDNGAHRLPRASKEVVARQLIAHIAK
                     LYKGASRKR"
     gene            7078..7752
                     /gene="radC"
                     /locus_tag="AC158Q_04830"
     CDS             7078..7752
                     /gene="radC"
                     /locus_tag="AC158Q_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HIR3339130.1"
                     /translation="MSIADWPLDDRPREKLIAKGAESLSDAELVAIFLRTGVRGRSAV
                     DLARDALTRFGSLSGLFAANAGALATVSGLGTAKYAQLQAVLEMARRALREKLDRGSA
                     LSSPQTVRDYLRLKLQDRPHEVFVAVFLDAQNRALAVEELFRGTLTQTSVYPREVVKR
                     ALHHNAAALIFAHNHPSGIAEPSRSDESLTQALKQALSLVDVKVLDHFVIGAGAAMSF
                     AERGLL"
     gene            7887..8336
                     /locus_tag="AC158Q_04835"
     CDS             7887..8336
                     /locus_tag="AC158Q_04835"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3339131.1"
                     /translation="MYKHVMLPVDGSELSQKAVSECIALLKSSGAKVTAVHVMSHFHL
                     HAQPWAPTRAMHEKIEKEHEEEARRAAQQMVDRLAQRVRAEGIDCEGLVVVGDQPYEE
                     IINHAEKRKCDLIIMASHGYKGLNAVLLGSETVKVLTHSKIPVLVVR"
     gene            8458..8694
                     /gene="rpmB"
                     /locus_tag="AC158Q_04840"
     CDS             8458..8694
                     /gene="rpmB"
                     /locus_tag="AC158Q_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HIR3339132.1"
                     /translation="MSRVCQVTGKKPMLGNNVSHANNKTRRRFLPNLQYRRIWVESEN
                     RWISIRLSQAGLRTIDRKGIDVVLAEMRARGESI"
     gene            8710..8865
                     /gene="rpmG"
                     /locus_tag="AC158Q_04845"
     CDS             8710..8865
                     /gene="rpmG"
                     /locus_tag="AC158Q_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIR3339133.1"
                     /translation="MREKIKLESTAGTGHFYTTTKNKRTTPDKMELKKFDPVARKHVI
                     YKETKIK"
     gene            complement(8997..10160)
                     /locus_tag="AC158Q_04850"
     CDS             complement(8997..10160)
                     /locus_tag="AC158Q_04850"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DesA family fatty acid desaturase"
                     /protein_id="HIR3339134.1"
                     /translation="MFTGVWDLPWWGYVVYALVVTHITIAAVTIFLHRCQAPRALDLH
                     PVVSHFFRFWLWLTTGMVTKNWTAIHRKHHARCETVEDPHSPQIEGINKVLWEGAELY
                     RKEAKNAETMEKYGRGNPDDWLERNLYSTHDRIGIGSMFVINFVLFGFVGISIWAIQM
                     AWIPFFAAGVINGIGHYWGYRTFQPEDASTNVVPWGIVIGGEELHNNHHAYPSSARFS
                     NRWFEFDIGWLYIRVMETLGLAQVKKVAPRIRLDAAKTRCDLDTVQAVITHRYEVIAK
                     YAHTLRQTCADEIHSLRARAVSVDWSAVKRTLHFGADALPANERARVDEALGMSKVLA
                     TVCTMRQELASLWQRSNASKEQLVRELEDWCARAEASGIIPLQEFSRRLRCYA"
     gene            complement(10426..11685)
                     /locus_tag="AC158Q_04855"
     CDS             complement(10426..11685)
                     /locus_tag="AC158Q_04855"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HIR3339135.1"
                     /translation="MSLTRTQLEAAAEALRLALKFDRPADSVLHDFFREQRALGGLDR
                     ALVADAVYGVLRRKRTVEYLAPEGAPRHLLLAYLARIAGVSIRELASAANPNEQSWLA
                     EVKAKSLEGLPLPVRAELPDWVVEQLGQHHTAAEILAIARGLNEPAPMDLRVNTLKAA
                     RDAVRHELAAGGIEAGTTPYSPAGLRVKGRPSINRHPLFLSGAIEVQDEGSQLLCHLV
                     GPRRHDLVVDFCAGAGGKSLMLGALMHSQGRIYAFDVSAARLNRLKPRLKRSGLSNLY
                     PHAIANENDIRVKRLAGKIDRVLVDAPCSGLGTLRRNPDLKWRQSPQSVEELKRKQAA
                     ILRAAARLLKPGGRLVYATCSLLAGENEDIVSAFLADRGDFRQLDCSEILREARIALD
                     TGPYFRLFPHAHGTDGFFAAVLEYEPR"
     gene            complement(11716..12405)
                     /locus_tag="AC158Q_04860"
     CDS             complement(11716..12405)
                     /locus_tag="AC158Q_04860"
                     /inference="COORDINATES: protein motif:HMM:NF022750.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HIR3339136.1"
                     /translation="MRLLAVMLAALSTALPAAAAPPSRVELTFAIMMGDLRIAEAREV
                     FEHDGRRYQVVSESTPKGIAALFINDVRRESRGAVTAAGLRPEHFEETGRRGGTRVAK
                     FDWTAGELTLVNSKTTRTVALPQGTLDQASLAYALAFRSTESAGFNVHVTDGRGVKQY
                     RYREIGVETLNTPLGELRTRRFEKVRDPGDKRGFEFWLATDRDLLPVKLRYLEKNGDV
                     FDSIITAITSR"
     gene            complement(12402..13046)
                     /gene="purN"
                     /locus_tag="AC158Q_04865"
     CDS             complement(12402..13046)
                     /gene="purN"
                     /locus_tag="AC158Q_04865"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017423116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HIR3339137.1"
                     /translation="MADKTIVILISGRGSNMEALLEARLPARVAAVISNDPRARGLGT
                     AQARGIATAVVDHRAYSDRAAFDAALAEEIDRHRPDLVALAGFMRILTDPFIERYRGR
                     LINIHPSLLPAFAGLHTHRRALEAGVRIHGCTVHFVTPALDSGPIVVQAAVPVLYGDT
                     EDRLAARVLAAEHRIYPQAVRWFIEGKLKLENGRVRIEDECRYPEAVVSPLAPR"
     gene            complement(13154..14233)
                     /gene="purM"
                     /locus_tag="AC158Q_04870"
     CDS             complement(13154..14233)
                     /gene="purM"
                     /locus_tag="AC158Q_04870"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HIR3339138.1"
                     /translation="MPQPFLRKRRLNKPGAPRPLTYRDAGVDIDAGDRLVENIKPFAR
                     KTLRQGVLAGIGGFGALFEIAAKKYRQPVLVSGTDGVGTKLKLAFELDRHATIGIDLV
                     AMSVNDILTLGAEPLFFLDYYACGRLDVATATRVVEGIARGCRQAGCALLGGETAEMP
                     GMYPAGEYDLAGFAVGIVEKSRIIDGTGIAEGDAVLGLASSGAHSNGYSLIRKIIAVK
                     GFDLSSKLDGETLADAILAPTRIYVKPVLKLVRSVAVRGLAHITGGGLVDNVPRILSG
                     RLAARIERAHWTMPPLFRWLQEQGGVADEELYRVFNCGIGMVVVVRDTDAGKALKTLR
                     AAGETAWRIGRIVKRKKNEPQVMLV"
     gene            14348..15070
                     /gene="hda"
                     /locus_tag="AC158Q_04875"
     CDS             14348..15070
                     /gene="hda"
                     /locus_tag="AC158Q_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HIR3339139.1"
                     /translation="MKQLALDFTSRPMATLDNFVVGRNGELLASLKRLAAREANERFL
                     YIWGRPGSGRSHLLKGTATELQRAGTAAAYVPCAADTRLAEGLERMDCVVLDDVDRLD
                     DEGQVAAFGLYNALREHNGALLAGGAAPPVQLKLREDLVTRLAWGLVYQVHALTDDEK
                     ARALADYAAGRGFHLVPEVCDYLLTRSSRDLSSLMATLDALDRYSLETKRPVTVPLAR
                     EVLHSIERRAPALGAGDTGSRG"
     gene            15067..15738
                     /locus_tag="AC158Q_04880"
     CDS             15067..15738
                     /locus_tag="AC158Q_04880"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HIR3339140.1"
                     /translation="MSERLVLFDLDNTLLAGDSDFEWAQFLIEQKVLDREVYEARNQQ
                     FYDQYKAGTLDIREFLDFQLKPLSRHPRAQLDAWHHDFMRSRIRPLIRGKARALVESH
                     RGDLCVMITATNSFVTAPIANEFGLANLVATEPEQVNGSFTGRVSGIPCFREGKVERL
                     EAWLAARGRKLASFSESWFYSDSLNDLPLLERVTHPVAVDPDPTLREHAGRKGWRVIS
                     LADGC"
     gene            15849..>17054
                     /gene="pcnB"
                     /locus_tag="AC158Q_04885"
     CDS             15849..>17054
                     /gene="pcnB"
                     /locus_tag="AC158Q_04885"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0043633 - polyadenylation-dependent RNA
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HIR3339141.1"
                     /translation="MITKFLGRVFSGRLFGPVQPKIISAGAHGIDRNLISPCAQRTVQ
                     ALQRSGFAAFVVGGAVRDLILGRKPKDFDIATDATPEEVRENFRRSRIIGRRFRLVHV
                     MCGPETVEVSTFRGAHQTEENDEGRADEHGRILRDNVFGNQEQDATRRDFTINALFYD
                     PATQEVWDYHGGVADLKRRQLRMIGDPERRYREDPIRMLRAVRLAASRGLEIDAATRR
                     PIHRLAGLLAHVPPSRLLDEMLKLLLSGHAADAVKQLRKEGLHHGVLPLLDAAMGRAA
                     GERFVMAGLAATDDRIRDGKTVSPGFLFASLLWPEVQAEWKVLEEKGMPAIPALYQAM
                     DHVMKAQAGKLPIPHRYGADMREIWAMQPRFLQRAGKRPYRLLEHPRFRASYDFLLLR
                     CQSGEADPAL"
BASE COUNT         3028 a         5422 c         5598 g         3006 t
ORIGIN      
        1 ccgagccgtc gagcctgctg tgcgtgttcg tcatcgccgc ggcgagcgcg gaggaggccg
       61 agcggctctc cgcgagcatc gccttgcgcc ggctcaacat ggattacggc gtcaacgcgc
      121 cggtgcccaa tcacgaggaa gcacggaatt atccgtatac cgaagccgac cgccgccgca
      181 tcgtccataa ccgccggcgc ctggtgctcg gcactcctgg cgttgttcgc gaccgcctgc
      241 tggagttgca gacgcggttc gaggccgacg aactgatggt catcaccatc accggcgact
      301 acgacagccg gctgcaatct tatgagctgc tcgcaggagc attcaacctg ggttgacggc
      361 aaaaggacct atttcatgaa ggtcatccgc gattgcctgg cggcgctgat tggcgcttta
      421 attccggcaa cgggaggagt tgtgcaggca cagggccagg acgcatacga agcgccgcgc
      481 aagcgcatga tggaggagat ctcggcgctc gtgcgcgaaa cgcgcctcga gaccggccgc
      541 gccgcgctgt ccgggcgcgt gatggcggcg atggcgaaag tgccgcgcca cgagttcgtg
      601 ccggacggcg aaaaacgcaa tgcctacgcc aaccggccgc tgcccatcgg cctggggcag
      661 accatctcgc aaccctacat cgtcgcgctg atgaccgatc tcatggaggt gaagcccggc
      721 gaccgggtgc tcgagatcgg taccggctcc ggctaccagg cggcggtgct ggcggagctt
      781 gccgaaacgg tttacaccgt cgagatcgtc gagccgctgg cgcgcgaggc gcgcgagcgg
      841 ctcaaggggt atcgcaacgt gatcacccga gcaggcgatg gctaccaggg ctggcctgaa
      901 cacgcgccgt tcgatgccat catggtgacc gcggcgccgc gcgaggtgcc gcaacccctg
      961 atcgatcagc tcaagcctgg cggacggctg gtggtcccgg tcggcggaca ggcgacggga
     1021 cagtcgctgc tgctgatcga aaagcggccg gacgggacgg tcacacggcg caacgtgctc
     1081 gcggtgcgtt ttgttccact caccgacaaa tcagggaaac aacaataatg aaaaagggcg
     1141 gctgagccgc ccttgcgttt gagtacgacc ggccacccgt caccggtcgc gccttgtcta
     1201 gaagcggtag cgcaccccga gtcgcaggag tccctgcagc cgcagcgggc tggccgcgtc
     1261 ctggggttgg agatcgtaac tgagggccca gctcggcgtg agccagtgct ggccggtgaa
     1321 catcacctgg cgctggcccg ggccgatggt atccaggaag ggcgtgaaag tttccacatc
     1381 gcgacccaac gccagcccga aggtgctgga ggcgctgtgc tggtagctca tttgcagctg
     1441 gtagctgggc gcaagcccgg cgccggatag tcgcgatggc agcagcgtgt agccgtttcc
     1501 ggtcggcatg tcgccagcca tgccatagcc cggtcccgag tccgcatcgc gcacccggta
     1561 cttgagcccc acgctcaatg ttcgtccctc gctgaacgag gcgtgcaact ggccggtaag
     1621 cgcgtagcgc cgaggtgacg ccggcgaacc ctggatgtat gccgcctcga gtgaggaacc
     1681 ccagccgccc ggcagggaat agacgatgcc cccgtaggat tccgcggcgc gcaacccgaa
     1741 attgggcgcc gcgcgcgccc ctccgtacag cgagtaatcc aaagagcgca tggtttgcca
     1801 gacttgcgcg gcttcgctgc cgaaagccag atccgccgaa actggcgcca tgtccgcctg
     1861 ggatctgagg ccaagacctg ccacgcgttc gggggacatc tgccccatgg ccgcgcccgg
     1921 agcgaacgcg aacagtgcaa tcaaggcaag attgtatggg tagcgcatag ggccagcgtg
     1981 attcagtgta agcctgacgc agccaaaccc ctcaaccaag tttaccaatc ctgtcattcg
     2041 cgttgcaaca ctgtggcgct ggcgcaacag gagtcagcct cgcccgcacc atcactaggc
     2101 gaattctctg cttcgcgcac catgatctgg cgcccatgac agcgggcact ttcaacctgt
     2161 tgaatccatg aactaaaaaa atggcacagc ggttgctagt acagaaccgg aagccccggg
     2221 cgtatcgcct tttgggattc cagagaggca tcgatgagcc tgacgcaggc gatcaagttg
     2281 ctgtccggtg aaatggccgg cgaacgcgaa ttgagcgaac gcgaggcatt cgcgctgatg
     2341 tcggcaatgc tcgacggggg catgggcgag ctggaactgg gcgcgctgct cgcgctgctc
     2401 gagcacaagc cggtgactct ttgcgagctg ctcggctatt gcgccgcgct ggcaccgcgc
     2461 tgcagccggc tgaagccgcc gagcgcccag tcacgacccg tcgtattcgc cgcgtaccat
     2521 ggagtgcgca gccagccgca cctgctgccg ctggtggcgc tcacgctgca gcgcctgggt
     2581 gtgccggtgc tggtgcacgg cgcgctcgat ggcgccggcg gtgttgctgc cgcgtacgtg
     2641 ttccgcgagc tgggcgtgct cccctgcgcg accctggccc aggcgcaatc gcggctcgat
     2701 gagcgcaagc tgaccttcgt tcctaccgcc gtgctggcgc cgggattcgc ggaattgctg
     2761 gcgctgaaag gccgcctggg tttcggcagc tttatccagg cgctcgcccg gttgctgatg
     2821 ccgttcgaag cggaggctct gtacgtggtc ggggtcaacc ccgtgacggg cggcgcattg
     2881 ctgcgcgaat atcttgcgat tagcggagcg cgtgcattgc tgctggaggg ggtggaaggg
     2941 gaagcgttcg cggatccccg gcgcaggcca cagatcgagt acgtggccac aggcgacttc
     3001 cgggtgctgt tcgacgcgga gaccgtcatg ctcaagcatt ccgcgacgct gccgttcgcg
     3061 gtcgatgcgc aaacggtggc ggcctggatt cgccgcgcca tggcggggga agtgccgctc
     3121 ccgctgccgc ttgtcaatca gctcgcctgc tgcctctacg gcgcgggcta taccgacgac
     3181 atgaaccagg ccaaggccat cgtcgccgtc gaaaccggca gcctcgctgc cgcctgaaat
     3241 ccgcgcaccg cttacgcgcg ccgccaggcg cgcgtggttt tccttcccgg atttccgacc
     3301 ggtattgagg tccggagcgc tggcgcatct gtgctagaat tttccgcagt cacagccaaa
     3361 aacactgaaa aggagcgcat ttatgggtgc gatattccag tcgttgggga agaccatcac
     3421 cgccggggtc gtgatattga tcgtgatcat tctcgcgtcg ggcggcgcgg gcgccaaggc
     3481 gggagatcac gcctggtggg cgttcgtgtt tcgctggctg cacgtgatgt cgggcgtcat
     3541 gtggatcggc ctgctgtggt atttcaaatt cgtgcagacg ccgtcgatgc cgaaaatccc
     3601 cgacgaacaa aagcccgcag tgagcaaggt aattgccccg acggcgctgt tctggttccg
     3661 ctggtccgcc ctggcgaccg tcgtgaccgg ccttatcgtc ggggggatga acggctacat
     3721 tgtcccggcg ctgatgctga ccaagggcgt gcacgcgatc gggatcggca tgtggctcgg
     3781 cctgatcatg gcgttcaacg tgtggttcat catctggccc aaccagaaga aggccctggg
     3841 catcgtccag gtgtcggctg aagagaaggc cgccgctgca aagctggccg gcatgacttc
     3901 acgcatcaac accatgttat ccataccgat gctgttctgc atggtcgcgc agcagaacgc
     3961 cggattgtga ccgcctgacc gtcgtcagcc gtacgcgaaa acgcccggga agtcccgggc
     4021 gtttttttcg aatcacgaat cgcaattcac gccgccgtca ccgggccggc ggcgcccgct
     4081 cgatgccctc gatcctcgcg cccggcttga tgccccgctt gcggaaccag ccttgattga
     4141 cctccagcgc gtacttggcg ggcttcgccg cggggtgcga attctgcgta tgcggcttca
     4201 tgtcctcgat gttgatgatc acgccgccct cgtcgagaaa cgccacggac agcgggatat
     4261 aggtgttcat catccacatc gcgtggagag caacgttgtc gaatacgaac agcatgccgc
     4321 ggctctcggg cagcatccgg cggtgcatca gtccctgcgt gcgcgaggaa ccggtatgcg
     4381 cgacttccgc cgtcagcttg tggccgttca ccgtgagaac gatctcagga agctgcgcgt
     4441 ccgccgcggc gggagccgcc agcgccagcg ctgccgctgc cagcgagcac ctaacggacc
     4501 gcaatgagat cgaattcata ttgaaccgcc aggacgccgg gaaaagcagg agaaatattt
     4561 gaatcgcgga gacgcggacg gaagcaaggt cggagaatag gcgcaagatg acatttgttt
     4621 tcaattcatt ctcgaagtgg attctcttgg cggccccggc ggttcgaaac gtctttaccg
     4681 cggcgcgctg tcgatcagcc attcggcaat cgcgttcagc agttcggtga cctcgccgat
     4741 cgggggcagc acgctgatat ccgcccccgg atgcctgttg tccaggtcct tcagcaggcg
     4801 cggcaggtcc ttcctgagat gtccgccctg cgcgagaaac agcggcgcga tgacgatgcg
     4861 ccggtgaccg gcgccggcca ggcgcgcgac gcagtcgcag agcgccggct ccatgatctc
     4921 gagaaaagcg agttccaccg tgaggtcggg gcggcgtgcc gagaccgctt tttgcagcgc
     4981 gcgaaacggc tcggcccatt gcgcgtcgcg ggcgccgtgc gcgaacagca ctaatgccga
     5041 tttttttgcg tcagccattt tcgtcttgat tgcagtgttt tcgccctcaa gcccgcgtga
     5101 atcgtgaatc gtgagtcgtg agtcgaacct gctgcctgaa agcagtggcg attcctgccg
     5161 cgtccaagct acgaatcacc attcaccaat caccaatcac gcatttcacc ggccggtgct
     5221 gccgaatcca cctgcgccgc gacggctcgc gccgaattct tccaccacgt tgaaggtcac
     5281 ctgcactacg ggcacgacca cgagttgggc gagacgctcc atcgggttga ccgtgaaggg
     5341 ggcgttgccg cgattccagg tggacacaaa gacctggccc tggtagtcgg agtcgatcag
     5401 cccgacgagg ttccccagca cgatgccgtg cttgtggccc aagcccgagc gcggcaggac
     5461 cacggccgcg aggccggggt cggcgaggtg gatggcgatg ccggagggga tcagctccgc
     5521 ctggccgggc gcgagcgtga caggggtgtc caggcaggcg cgcaagtcga gcccggccga
     5581 ccccggcgtc gcatactgcg gaagctgccc gcgcaggcgc tcgtcgagga ttctgacgtc
     5641 gatcttcttc atgaacaggt gagcagcagg cggtaagcag tgagctgtcg gagcagaacg
     5701 gtcttaaccg cttaccgctt acggcttgcc cctttatata acttggcgat atgggcgatg
     5761 agttgccgtg ccaccacttc cttggacgcg cgcgggagcc tgtgggcgcc attgtcgtcg
     5821 aacagcgtga cctcgttctc atcggagccg atcgcctcct gcgcgaggtt ggcgaccatc
     5881 agcggcactt tcttgcggcg ccgcttttcg tcggcatagg catcgaggtt gcggctctca
     5941 gcggcaaagc cgacgcagaa cggcggcctg ggccgcgcgg ccacccaggc gaggatgtcg
     6001 ggattgggca cgagcttcag gtcgagccgt ttgccctcgg tcttcttgat cttgtgccgg
     6061 ctcggctttt ccacgcggta gtcgcccacc gcggcaacgc tgatgtagat atcggcgcgc
     6121 gcggcatggc gcttgacggc atcgaacatt tcggcggcgg tgccgacccg gctcatctcg
     6181 gctcccgccg gcgccggcag gctcacgggc cccgatacca gggtgaccct ggcgccagcg
     6241 tccagggcgg cgcgcgcgac cgcgtatccc atcttccccg agctgcggtt ggtgatgccg
     6301 cgcacggcgt cgatcggctc gaaggtcggg ccggcggtga tcagcacgcg cgcgccggcg
     6361 agcagccgcg gtgccagaaa tccggcgacg gactccgcaa tctcatgcgc ttcgagcatg
     6421 cggcccatgc cgacctcacc gcacgcctgg tctccgcttg cgggacccag gatcgccacg
     6481 ccgtcgcgca gcagccggtc gacgtttcgg cgcgtcgcgg ggttctgcca catctggcgg
     6541 ttcatggcag gggcaacgat cagcgggcac tcgcgcgcca ggcacagcgt cgagagcaaa
     6601 tcgtccgcca gcccgctcgc gagcttggcg atgaaatccg ccgtggcagg cgcgatgacg
     6661 atcgcgtcct tgccgcgcga gagctcgatg tgcgccatgt tgtcggggac gcccgcgtcc
     6721 cacatgtcgg tgtagaccgg ttttccggac agcgcctgga acgtggccgg cgtgaccatg
     6781 ccccgcgcgg cttcggtcat caccacctgg acatcgatgc cgtggcgctg gaggtcgcgc
     6841 gtcagctgcg ccgctttgta cgcggcgatg cccccggtta ccccgagaac gactgatttt
     6901 ccagatcttt cagccatgta gcgcttcaac ttcaagtggg gggtaagagt gtaaccgcaa
     6961 acgccgtgaa ccgtaaatcg ttaatcgtga accgaaaagt gcacctggta aacgcgaatc
     7021 tggtctgccc cgttagtggc ttaacgattc actgctcacc cttcacgatg cacgtttatg
     7081 tccattgccg actggccgct cgatgaccgc ccgcgcgaga agttgatcgc gaagggagcc
     7141 gagtccttgt ccgacgccga gctggtggcg atctttctgc gcaccggcgt ccgcggaaga
     7201 agcgcggtgg atctcgcccg cgacgcgctc actcgcttcg gctcgctctc cgggctgttc
     7261 gccgccaatg ccggagcgct cgccacggtg tcggggctcg ggacggcaaa atacgcccag
     7321 ttgcaggcag tgctcgagat ggcgcggcgg gccttgcgcg agaaactcga ccggggctcg
     7381 gcattgagtt cgccgcagac cgtgcgcgac tatctgcggc tgaagctcca ggaccggccg
     7441 cacgaagtat tcgtggccgt tttcctcgac gcccagaacc gcgcgctggc ggtcgaggag
     7501 ctgttccgcg ggacgttgac ccagaccagc gtttacccgc gcgaggtggt aaaacgcgcc
     7561 ctccaccaca acgcggccgc cctgatcttc gcccacaacc atccttcggg catcgcggag
     7621 ccgagccgtt ccgacgagtc tctcacccag gcgctcaagc aggcgctgtc gctggtggac
     7681 gtcaaggtgc tcgaccactt cgtaatcggg gcgggggcgg cgatgtcgtt tgccgagcgc
     7741 ggcttgttat agaaccttga cctagatcaa ccggtcgagg cggcgtgcac gcacgccggc
     7801 cccgattgcc ttaggatgat tcgcgcagga atcgccggca agtcattgcg gcctgccggt
     7861 attgcgcata tcaggaaagg gcgcccatgt acaagcacgt catgttgccg gtcgatggat
     7921 ccgaactatc gcagaaggca gtcagcgagt gcatcgccct gttgaaatcc agcggcgcga
     7981 aggtgaccgc ggtacacgtg atgtcgcatt tccacctgca tgcccagccg tgggccccga
     8041 cccgggccat gcacgagaag atcgagaagg aacacgagga ggaagcccgg cgggccgcgc
     8101 agcagatggt cgacaggctg gcgcagcgcg tccgggcgga ggggatcgat tgcgaaggcc
     8161 tggtggttgt cggtgatcag ccgtacgagg agatcatcaa ccacgcggaa aagcggaaat
     8221 gcgatctgat tatcatggct tcgcacggtt acaaggggct gaacgcggta ctgctcggca
     8281 gtgaaaccgt caaggtgttg acgcatagca agatccccgt gctggtcgtg agataggcaa
     8341 agccccgttt cgacggaacc tcgggcgccg ggacggggtc ggggcgttgc ggccgcgctt
     8401 gagcggggct cgaaaaccct gtagaatccg cgcctttcct gaattccgga gcactccatg
     8461 tcacgcgtct gccaggtcac cggcaaaaaa ccgatgctgg ggaacaacgt gtcccacgcc
     8521 aacaacaaga cgcggcggcg cttcctgccg aacctgcagt accgccgcat ctgggtcgag
     8581 agcgagaacc ggtggatcag catccgcctg tcccaggccg gcctgcgcac gatagaccgc
     8641 aagggaatcg acgtggtgct ggctgaaatg cgcgcccgcg gcgagagcat ctaggaaagg
     8701 agccgaacca tgcgtgaaaa gatcaagctc gaatcgaccg ccggcaccgg ccatttctac
     8761 accaccacca agaacaagcg caccaccccg gataaaatgg aactgaagaa gttcgatccc
     8821 gtggcgcgca agcacgtcat ctacaaggaa acaaaaataa aataggcccg gcgccgtcga
     8881 ggccggtcgg gcggacgcag gcaccactca acgcccggca gtagaaacaa aaaagcccgc
     8941 ctaggcgggc ttttttacgt accttgaaac ttgaaactcg aaacttgtaa ccgggctcaa
     9001 gcgtagcaac gcaggcggcg gctgaattcc tgtagcggaa tgatgccgct cgcttcggca
     9061 cgcgcgcacc agtcttcgag ttcgcgcacc agctgttcct tggacgcgtt cgagcgctgc
     9121 cacagcgacg ccagttcctg gcgcatggtg cagacggtgg ccagcacctt gctcatgcca
     9181 agcgcttcgt caactcttgc gcgctcgttg gcaggcagcg catccgcgcc gaaatgcagc
     9241 gtgcgtttca cggccgacca gtccacgctc accgcgcgcg ccctcaggct gtggatctca
     9301 tcggcgcagg tctggcgcag cgtatgcgcg tacttggcaa tcacttcgta gcggtgcgtg
     9361 atcaccgcct gcacggtatc gaggtcgcag cgcgtcttcg cggcatcgag cctgatcctg
     9421 ggcgcgacct tcttgacctg ggcgagaccc agagtttcca tgacgcggat ataaagccag
     9481 ccgatgtcga actcgaacca gcggttggaa aagcgcgccg aactcgggta ggcgtggtga
     9541 ttgttgtgca gttcctcgcc gccgatcacg atgccccacg gcacgacgtt ggtgctcgca
     9601 tcctcgggct ggaaggtgcg gtatccccag tagtgcccga tgccgttgat gacgccggct
     9661 gcgaagaagg ggatccaggc catctgtatg gcccagatgc tgatgccgac gaagccgaat
     9721 aggacaaagt tgatgacgaa catgctgccg atgccgatcc ggtcgtgcgt cgagtagaga
     9781 ttgcgctcga gccagtcatc ggggttgccg cggccgtact tctccatggt ctcggcgttt
     9841 ttcgcttcct tgcgatacag ctccgcgcct tcccacagca ccttgttgat gccctcgatc
     9901 tgcgggctgt gcgggtcttc cacggtctcg caccgggcgt ggtgcttgcg gtggatcgcg
     9961 gtccagttct tcgtgaccat gccggtggtc agccacagcc agaaacggaa gaaatggctt
    10021 acgaccggat gaaggtccag ggcccggggc gcctggcagc ggtgcagaaa aatggtgacc
    10081 gcggcgatcg tgatgtgggt gacgacgagg gcatagacca cgtagcccca ccacggcaga
    10141 tcccagaccc ctgtaaacat gaatttcccc ggaggaagaa ttggaatagt taatgtgcgt
    10201 ccagtttacc cataatcaac ttgcaggcat agcgtagatt gctcagtgca ttgatagttc
    10261 taagatttat aaattaaacg cgttcccgta ttgtgaaatt atgcacaata gcggccgcaa
    10321 ccgcttgcag cggtgtgcga acgatgccgt gatacccggc gcctggaatg cgcgcccgac
    10381 cccggcgcgg agatcggcgc gccggcagat cggtccctga gcagttcagc gcggctcgta
    10441 ttccagcacc gctgcgaaaa aaccgtcggt gccatgcgcg tgcgggaaca gcctgaaata
    10501 cgggccggta tcgagtgcga ttcgcgcctc gcgcaggatt tccgagcagt cgagttgccg
    10561 gaagtcacca cgatcggcca gaaacgccga gacgatgtcc tcgttctctc cggcgagcag
    10621 gctgcaggtc gcgtagacca ggcggccgcc gggcttcagc agccttgccg ccgcgcgcag
    10681 gatcgcggcc tgcttgcgct tcagttcctc caccgattgc ggtgactggc gccacttgag
    10741 gtcgggattg cggcgcagcg tgccgagtcc gctgcacgga gcatccacca gcacgcggtc
    10801 gatctttccc gcgagccgct tcacgcggat gtcgttctcg ttggcgatcg catgcggata
    10861 gaggttggaa agaccggagc gcttgagcct gggcttcaag cgattcagcc gcgcagccga
    10921 gacgtcgaat gcatagatgc ggccctggga atgcatcagc gctccgagca tgaggctctt
    10981 gccgcccgcg ccggcgcaga agtccacgac caggtcatgg cgccgcggcc cgacgaggtg
    11041 gcacagcagc tggctgcctt cgtcctggac ctcgattgcg cccgacagga acaacgggtg
    11101 ccggttgatc gagggccggc ctttgacgcg caggccggcg ggcgagtacg gcgtggtccc
    11161 ggcttcgatg ccgcccgccg ccagttcatg ccgcactgca tcgcgtgcgg ctttcagcgt
    11221 attcacccgc agatccattg gcgccggctc gttcaggcca cgagcgatcg ccagaatctc
    11281 cgctgcggta tggtgctggc caagttgttc cacaacccaa tccggcagtt cggcgcgcac
    11341 cgggagcggc aggccttcga gcgatttcgc cttgacctcg gcaagccatg actgctcatt
    11401 cggattggcc gcgctcgcaa gctcgcggat gctgacgccg gcgatgcggg ccaggtaggc
    11461 gagcagcagg tgccgcggcg cgccctccgg cgccaggtat tccacggtgc gcttgcggcg
    11521 cagaacgccg tagaccgcat cggctacgag cgcccgatcc agccccccca gtgcgcgctg
    11581 ctcgcggaag aaatcatgca ggaccgagtc ggcgggccgg tcgaacttga gcgcaagccg
    11641 cagtgcttcg gccgccgctt cgagctgcgt gcgagtcagg ctcacggaaa attcgaatcg
    11701 gcgggactga ccggattacc gcgacgtgat ggccgtgatg atcgagtcga atacgtcgcc
    11761 gttcttctcg aggtagcgca gcttcacggg gagaagatcg cggtccgtcg cgagccagaa
    11821 ctcgaacccg cgtttgtcgc ccgggtcgcg cactttctcg aaacggcgcg tcctgagttc
    11881 gccgagcggc gtgttgagcg tttccacgcc gatctcgcgg taacgatact gcttcacgcc
    11941 gcgcccgtcg gttacgtgga cgttgaagcc cgcgctttcc gtggaccgga aggcgagcgc
    12001 gtaggcaaga ctcgcctgat cgagcgtgcc ctgcggcagc gccacggtcc gggtggtttt
    12061 gctattgacc agcgtgagtt cgcccgcggt ccagtcgaac ttcgcgaccc gggtgccgcc
    12121 tctgcgtccg gtttcctcga aatgttcggg cctgagtcca gccgcggtaa cggcgccgcg
    12181 gctttcgcgg cgaacgtcgt tgatgaacaa ggcagcgatc cccttcggcg tagactcgct
    12241 gacgacctgg tagcgccggc cgtcgtgctc gaacacttcg cgtgcctccg cgatccgcag
    12301 gtcccccatc atgatcgcaa acgtgagttc gacgcgcgag gggggcgcgg cggcggcggg
    12361 aagcgccgtg ctcaacgcgg cgagcatgac ggcgagcagt ctcatcgggg agccagcggg
    12421 gagacgaccg cctccgggta ccggcattcg tcttcgattc tcacccggcc gttttccagc
    12481 ttgagcttgc cctcgatgaa ccagcgcacg gcttgcgggt agatgcgatg ctctgcggcc
    12541 agcacccgtg cagcgagccg atcctctgtg tccccgtaca acaccggcac ggccgcctgc
    12601 acgacgatcg gcccgctgtc gagcgccggg gtcacgaagt gcacggtgca gccatggata
    12661 cgcacgccgg cctcaagtgc ccggcggtgg gtgtgaagcc cggcgaacgc gggaagcagg
    12721 gaagggtgga tgttgatgag ccgcccgcga tagcgctcga tgaaaggatc cgtgagtatg
    12781 cgcatgaagc ccgcgagcgc gacgagatcc ggccggtgcc ggtcaatttc ctccgccagt
    12841 gcggcgtcga aagcggcgcg gtcggagtac gcgcggtgat cgaccaccgc ggtggcgatg
    12901 ccgcgcgcct gcgccgtgcc gaggccgcgc gcccgaggat cattgctgat gaccgccgca
    12961 acccgcgccg gcagccgcgc ttccagcaac gcttccatgt tgctgccgcg gcctgatatg
    13021 agtatgacga tggtcttgtc agccatggtt ttagttttga gttttgagtg tttagtgttg
    13081 agttcgattc aacctgacct cgatttcagc tttgcaactc aacattcaac actcaacact
    13141 aaacactggt tcattagacc agcatgacct ggggttcgtt ctttttccgt ttgacgatgc
    13201 gcccaatgcg ccaggcagtt tcgccggccg cccggagcgt cttcagcgct ttgccggcat
    13261 cggtgtcacg gactaccacg accatgccga tgccgcagtt gaatacacga tagagctcct
    13321 catccgcaac gccgccctgt tcctgcagcc atcgaaacag cggcggcatc gtccagtgcg
    13381 cgcgctcgat gcgtgccgcg agccgcccgg acaggatgcg cggcacgttg tccacgagcc
    13441 cgccgccggt gatgtgggcg aggcctctga ccgccacgga acgcacgagc ttcagcaccg
    13501 gcttcacgta aatgcgtgtc ggcgcgagta tggcgtcggc gagcgtttcc ccgtcgagct
    13561 tcgacgacag gtcgaagccc ttgaccgcga tgatcttgcg gatgagcgag tagccgttcg
    13621 aatgcgcgcc gctggaggcg aggcccagca cggcgtcgcc ctcggctatg cccgtgccgt
    13681 cgatgatgcg gcttttctca acgatgccga ccgcgaatcc ggcgaggtcg tactcgcccg
    13741 ccggatacat tccgggcatt tcggcagtct cgccccccag caacgcgcag ccggcctgcc
    13801 tgcagccgcg ggcgatgccc tccacgaccc tggtcgccgt cgcaacgtcg agccggccgc
    13861 aggcgtaata gtcgaggaag aacaggggtt cggcgccgag cgtcaggata tcgttcacgc
    13921 tcatcgccac gaggtcgatg ccgatggtgg catggcggtc gagttcgaag gcgagcttca
    13981 atttggtgcc cacgccgtcg gtgccggaca ccagcaccgg ctgccggtat ttttttgccg
    14041 ctatctcgaa cagcgcgccg aaaccgccga tgccggcgag cacgccctgg cgcagggttt
    14101 tcctggcaaa tggcttgatg ttctcgacca gccggtcgcc ggcgtcgata tccacgccgg
    14161 catcacggta ggttagcggt cgcggggcgc caggtttatt caagcggcgt ttccgtaaga
    14221 aaggctgggg catttggtaa gataggcgcg cagccgaggc agccgtcccg ccttctgcgt
    14281 cttccgcgcg gtccaaggca agcgaattct accgtaaata cagacgcaac cggtccctcc
    14341 cggcaccatg aaacaactcg cactcgattt caccagccgg cccatggcga cgctggacaa
    14401 tttcgtcgtg ggacggaacg gcgagctgct cgccagcctg aaacggctcg ccgcacgcga
    14461 ggcgaatgag cggttccttt atatctgggg acggcccggc agcggccgca gccatctgtt
    14521 gaaaggcacc gcgacggaac tgcaacgcgc ggggacggcc gcggcatacg ttccgtgcgc
    14581 ggccgatacc cggctcgcgg aggggctcga gcgcatggat tgcgtcgtgc tcgacgacgt
    14641 cgaccgactc gatgacgagg gacaggtcgc cgcgttcggc ctgtacaacg ccctgcgcga
    14701 gcacaacggc gcgctgctgg cgggcggcgc cgcgccgccg gtgcagctga agctgcgcga
    14761 ggacctggtc actcgcctgg cctggggcct ggtctaccag gttcatgcgc tgacggacga
    14821 tgagaaagca agggcgctcg ccgattacgc cgccggccgg ggtttccacc tcgtgcccga
    14881 ggtgtgcgat tacctgctga ctcgatcgag ccgcgacttg tcctcgctga tggcgaccct
    14941 ggacgcgctc gaccgctatt cgctggaaac caagcgcccg gtcaccgtgc cgctcgcgcg
    15001 cgaggtgctg cactcgatcg agcggcgtgc cccggcactg ggcgccgggg atacggggag
    15061 ccggggttga gcgagcggct ggttctcttc gacctcgaca atacgttgct ggcgggagac
    15121 agcgatttcg agtgggcgca gttcctgatc gagcagaagg tgctcgatcg cgaggtgtac
    15181 gaggcgcgca accagcagtt ctacgaccag tacaaggcgg gaacgctgga cattcgcgag
    15241 tttcttgatt tccagctgaa gccgctgtcg cgtcaccctc gcgcgcagct cgatgcctgg
    15301 catcacgact tcatgcgcag caggatccgc ccgctgatac gcggcaaggc ccgcgcgctc
    15361 gtcgagagcc atcgcggcga cctgtgtgtc atgatcaccg ccaccaacag tttcgtcaca
    15421 gcgccgatcg cgaacgagtt cgggctggcg aacctggtcg cgacggagcc tgagcaggtg
    15481 aatggcagct tcaccgggcg cgtgtccggc attccgtgct tccgcgaggg caaggtcgag
    15541 cggctggaag cctggcttgc cgcgcgcggc aggaagcttg cctcgttttc cgaatcgtgg
    15601 ttctacagcg attcgctgaa cgacctgccg ctgcttgagc gcgtcaccca tcccgtggcg
    15661 gttgacccgg acccgacgct gcgcgagcat gccgggcgca agggctggcg cgtcatcagc
    15721 ctggccgacg ggtgttgagt gatgagtttt gaatgtttgg ttgtaaccac aagaatcgct
    15781 gcaatgccac aatctgcctg gcgcccgcca ctcaacacta agcactcaac actcaacact
    15841 gggttcacgt gatcaccaaa ttcctcggcc gggtcttctc cggccgcctg ttcggtccgg
    15901 tgcagccgaa gatcatttca gccggcgcgc atggcatcga tcgcaatctg atcagcccgt
    15961 gcgcacagcg cacggtacag gcgctccagc gcagcgggtt tgccgccttc gtggtcggcg
    16021 gcgcggtgcg cgacctgata ctcggccgca agccgaagga tttcgacatt gcaaccgatg
    16081 ccacgcccga ggaagtgcgg gagaatttcc gccgctcgcg catcatcggg cgccgattcc
    16141 gcctggtgca cgtgatgtgc ggtccggaaa cggtggaggt gtccacgttc cgcggcgccc
    16201 accagaccga ggagaacgac gagggcaggg cggacgagca cggacggata ctgcgcgaca
    16261 acgtgttcgg gaaccaggag caggacgcca cccgccgcga tttcaccatc aacgcgctgt
    16321 tctacgaccc ggcgacgcag gaggtctggg attaccacgg cggcgtcgcc gacctgaaga
    16381 ggcggcagct acgaatgatc ggcgacccgg agcgacgcta tcgcgaggac ccgatccgca
    16441 tgctgcgcgc ggtgcggctc gcggcgagcc gcgggctgga gatcgacgca gccacgcgcc
    16501 gtccgatcca tcgactggcg gggctgctcg cgcacgtgcc gccatcgcgg ctgctcgacg
    16561 aaatgttgaa gctcctgctg tccggccacg cggcggatgc ggtgaagcag ttgcgcaagg
    16621 agggcctcca tcacggggtg ctgccgctgc tcgatgcggc catggggcgg gcggcgggcg
    16681 agcggttcgt gatggcgggg cttgcggcta ccgacgatcg catccgcgac gggaagacgg
    16741 tgtcaccggg tttcctgttc gcttcgctgc tttggcccga agtccaggcc gagtggaagg
    16801 tgctggaaga aaagggcatg ccggcgatcc cggcgctcta ccaggcgatg gaccacgtaa
    16861 tgaaggccca ggcggggaag cttccgatcc cgcaccgtta cggcgccgac atgcgggaga
    16921 tctgggcgat gcaaccgcgt ttcctgcagc gggccggcaa gcgcccgtac cggctgctcg
    16981 agcacccgcg gttccgcgcc agctacgact tcctgctcct gcgctgccag agcggcgaag
    17041 cggacccggc gctc
//
LOCUS       DBNGSG010000186         4080 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1082182, whole genome shotgun sequence.
ACCESSION   DBNGSG010000186 DBNGSG010000000
VERSION     DBNGSG010000186.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1082182"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /locus_tag="AC158Q_04890"
     CDS             <1..1054
                     /locus_tag="AC158Q_04890"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR3339142.1"
                     /translation="PERDAAVVAMLRAAGAGIMGKTVTTKCATYTAGKTRNPHNPEHT
                     PGGSSSGSAAAVAAGMVPIALGSQTNGSVIRPATFCGVYGFKPTHGLIPRTGILKLSR
                     ALDHVGVFARTIEDVALAAEQLVGCDEDDLDTRPRARPPYREVVAQEPPLPPRIAFVK
                     GPAWERAEEETTQAFAELTAELGDRVVEVELPGTALRALEWHRTIMEAEMAANLDLEW
                     EKGRERLSESLRNQLARGREVRALDYQAALARIPLLNDGFAELFSEYDAILTPAAPGT
                     APKGLGATGDPAFCTLWTLCGMPAISLPLMEGENGLPLGVQLVGPRHGDARLLRTARW
                     LAGQIEGNRESTGGNP"
     gene            1054..1221
                     /locus_tag="AC158Q_04895"
     CDS             1054..1221
                     /locus_tag="AC158Q_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339143.1"
                     /translation="MAIKVAAALVAVILLSGYLLALAFKLKEIDLAIVIAIGLALMLV
                     DLWQSFKSKED"
     gene            1367..2524
                     /locus_tag="AC158Q_04900"
     CDS             1367..2524
                     /locus_tag="AC158Q_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR3339144.1"
                     /translation="MAYPHNKKPLRQSVTARRRFLTVAAAAAAAFPMTAEAQSPAVLR
                     FQGAWSAKDVFHEYALDYARKVIDMSGGRVRIEVLSAGAVVKPPELLDAVHKGLLDGC
                     HAVPAYWAGKDSAFSLFGSGPALGMDAALLLSWMDYGGGKALYEELYSRMMLSNVTGF
                     LYGPMPTQPLGWFRKPLTSAAQLKGLRVHARGLPAQLFREMGAVVRELPDDEIVPAAR
                     RGLLDGVAFNNATSDRQLGLPDLFPVCMQRSYHQPAQVFEVILNRKRFDSMPADLRAI
                     LRYAAQAASADLSSKAAHRYSADYAALRGQSSIRFLNTPPEVLRAQLRAWDVLAARAM
                     RDNPFFEKVLNSQQVWARRAAGWALDTLVDPRLAYDHWFARQAPGAPAGKS"
     gene            2527..3813
                     /locus_tag="AC158Q_04905"
     CDS             2527..3813
                     /locus_tag="AC158Q_04905"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3339145.1"
                     /translation="MAGGAAAWTLRRGWVVFGLAAAAFWLSFFHRVAPAAIAVELQHA
                     FGIGGAMLGALAATYYVIYTLMQVPTGVLNDTIGPRRVLAAGCLVAGAGSLLFGTAET
                     VTAAVVGRTLAGLGVAVAFVSMLKLNADWFHERRFATMTGFGAMIGLTGALAAATPLA
                     WISTFVSWRSVFVAAGIASLLLAAAIWVAVRDRPEGAATLAPRAPVEGPRWRAGLMMV
                     VRNRATWPCFWYGFGMSGSYMTFVGLWAVPYLVHGYGLSVVEATQHTSLMLVCLAASQ
                     GVIGALSDRIGHRRPLILASALAYFACWLAWLSGAAAMPGAAYAISIVMGLSVCGMTL
                     SWACAKELNSPDYSGIAISVVNTSGFLAVGLLQPLVGWLLDRAAAGEAYTGAHYLPGI
                     ALLTAFAGIALAGALFIRETHCRNIWPELNPQRGVA"
BASE COUNT          596 a         1370 c         1413 g          701 t
ORIGIN      
        1 gccggagcgc gatgccgccg tcgtcgcgat gctgcgcgcc gcgggcgcgg ggatcatggg
       61 caagaccgtc accaccaagt gcgccaccta tacagcgggc aagacgcgca atcctcacaa
      121 ccccgaacac acgccgggcg gctcctcgag cggctcggcg gccgcggttg ccgccggcat
      181 ggtgcccatt gcgctgggca gccagaccaa cggctccgtg atccgtcccg ccacgttctg
      241 cggagtgtac gggttcaagc cgacgcacgg cctgatcccg cgcaccggca tcctgaagct
      301 ctcccgcgcg ctcgatcatg tcggcgtatt cgcgcgcacg atcgaggacg tcgcgctggc
      361 cgcggaacag ctggtcggct gcgacgagga cgatctcgac acacgaccgc gcgcgcgccc
      421 gccgtaccgc gaggtcgtcg cccaggagcc gccgctgccg ccgcgcattg cctttgtaaa
      481 ggggccggcc tgggagcggg cggaggagga aaccacgcag gcgtttgccg aactgacagc
      541 cgaactgggc gaccgtgtcg tggaggtcga acttcccggt accgcgctac gcgcgctcga
      601 gtggcaccgc acgatcatgg aagccgaaat ggcggcgaat ctcgacctgg agtgggagaa
      661 ggggcgggag cgcctgtccg agtcgctgcg caaccagctt gcccgggggc gcgaagtgcg
      721 ggcgctcgat tatcaggcgg cgctggcgcg tattcctctt ctgaacgacg gattcgcgga
      781 gctgttttcc gagtacgatg cgatcctgac cccggcggcc ccaggcaccg cccccaaagg
      841 cctgggcgcg accggcgacc cggcgttctg cactttgtgg accctgtgcg gcatgccggc
      901 catcagcctg ccgctgatgg agggcgagaa cggcctgccg cttggcgtgc aactcgtcgg
      961 gccgcgtcat ggcgacgcgc gcctgctgcg caccgcgcgc tggcttgccg gacagatcga
     1021 aggcaatcgg gaatcaaccg gagggaaccc gtaatggcaa tcaaggtcgc ggcggcgctg
     1081 gtggcggtaa tcctgttgtc cgggtatctc ctggccctgg cattcaagtt gaaggagatc
     1141 gacctcgcga tcgtgatcgc catcggcctg gcgctgatgc tggttgacct ctggcagtcg
     1201 ttcaaatcca aggaagactg atcgcgtggt tttccggcct gcatgagccg ggcgcgccta
     1261 tccgcgcacg gtatcctttt ccgctgaaca acccgcaggc gcggcggcgg ctgtccgcat
     1321 cgcgcatccg accgtaacga gctcctcccc gaacgagacg ccgagaatgg cctacccgca
     1381 caacaagaag cccttgcgcc agtccgtgac ggcccgccgc cgctttctga ctgtcgcggc
     1441 agcggccgcc gccgccttcc cgatgaccgc cgaggcgcaa tcacccgccg tcctgcgctt
     1501 ccagggggcc tggtcggcga aggacgtctt tcacgagtac gcgctcgatt acgcccggaa
     1561 ggtgatcgac atgagcgggg ggcgcgtgcg catcgaggtg ctgtccgcgg gcgccgtggt
     1621 gaagccgccg gaactgctcg atgcggttca caagggcttg ctcgacggct gccacgcggt
     1681 tcccgcgtac tgggccggca aggactcggc gttctcgctg ttcggctccg gtcccgcgct
     1741 gggcatggat gcagccctgc tgctgtcgtg gatggattac ggcggcggaa aggcgctgta
     1801 cgaggagttg tattcccgca tgatgctttc gaacgtgacg ggatttctct acgggccgat
     1861 gccgacgcaa ccgctcggct ggtttcggaa gcccctgacc tcggccgccc aactgaaggg
     1921 gctgcgggtt cacgcgcgcg gtttgcccgc gcagctgttc cgcgagatgg gggcggtggt
     1981 ccgggaactg cccgacgacg agatcgtccc ggcggcgcgt cgcggcctgc tcgatggcgt
     2041 ggcgttcaac aatgccacgt cggaccgaca attgggcctg cctgacttat tccccgtctg
     2101 catgcagcgc agctatcacc agccggcgca ggttttcgag gtcattctca accggaagcg
     2161 gttcgactcg atgccggccg atctccgcgc gatcctgagg tacgcagcgc aggcggcgag
     2221 cgccgacttg tcatcgaagg ccgcccatcg ctattccgcg gattatgccg cactgcgcgg
     2281 gcagtccagc atccgcttcc tgaacacgcc gccggaagtg ctgcgcgcgc agctccgggc
     2341 atgggacgtc ctggccgcgc gcgcgatgcg cgacaatccg ttcttcgaaa aagtgctcaa
     2401 ctcgcagcag gtctgggcgc gccgcgccgc cggctgggcg ctcgacaccc tcgtcgatcc
     2461 gcgcctcgcc tacgatcact ggttcgcgcg gcaggcaccc ggcgcccctg cagggaagtc
     2521 ctaggcatgg cgggcggcgc ggccgcgtgg acgttgcgcc gcggctgggt ggtattcggg
     2581 ctcgcggccg ccgcgttctg gctgtccttc ttccatcgcg tcgcgcccgc tgcgatcgcg
     2641 gtcgagcttc agcacgcatt tggtatcggg ggcgcgatgc tcggcgcgct cgccgcgacc
     2701 tattacgtga tctacacgtt gatgcaggtg ccgaccggcg tgctgaacga cacgattggc
     2761 ccgcgccgcg tgctggccgc gggctgcctg gttgcgggag caggctccct cctgttcggc
     2821 acggcggaga ccgtgacggc ggcggtggtc ggccggacgc tggcgggcct gggcgtagcg
     2881 gtggccttcg tgtcgatgct caagttgaac gccgactggt ttcacgagcg ccgcttcgcc
     2941 accatgaccg gtttcggcgc catgatcggc ctcaccggcg cgctggccgc ggccacgccg
     3001 ctcgcatgga tatcgacgtt cgtctcctgg cgcagcgtgt tcgtcgcggc gggcatcgcg
     3061 tcgctgctgc tggccgccgc aatctgggtt gccgttcggg atcgtccgga aggcgcggcg
     3121 acccttgcac cgcgcgcgcc cgtcgagggc ccgcgctggc gcgcaggctt gatgatggtc
     3181 gtgcgcaacc gggcgacctg gccgtgcttc tggtacggct tcggcatgtc cggcagctac
     3241 atgacatttg tcggcctgtg ggcagtgccg tacctggtgc acggctacgg cctgtcggtg
     3301 gtggaagcca cgcagcacac gagcctgatg ctggtctgtc tagcggcctc ccagggcgtc
     3361 atcggcgcgc tgtcggaccg catcgggcat cgccgccccc tcatcctggc ctccgccctg
     3421 gcgtatttcg cctgctggct cgcgtggctg tccggggccg ctgccatgcc cggcgcggcc
     3481 tacgccatca gcatcgtgat ggggttgagc gtctgcggca tgacgttgtc gtgggcatgc
     3541 gccaaggaac tgaattcccc ggattactcc ggaatcgcga tcagcgtggt caacaccagc
     3601 ggttttctcg cggtcgggct gctgcagccg ctggtgggct ggctgctcga ccgcgccgcc
     3661 gccggtgaag cctataccgg cgcccactac cttcccggca tcgcgctgct gacggctttc
     3721 gcgggcatcg cgctggcggg cgcgctgttt atccgggaga cgcactgccg taatatctgg
     3781 ccggaactga atccgcaaag gggcgtcgca tgacccgcgg agcaggggcc ccgcttgcgg
     3841 ggatgcggcc ggtgaaggcg actggccgcc gaccagatga aaaggatatg actggaatga
     3901 tggcggaggc ggcatgaaac cgaagatcct cgttacccgt gaagtgttcg acgaaacgct
     3961 ggcctttctc gcaatgcact gcgaggtcga atccaaccag caggacgccg cgctggctcc
     4021 cgaagcgctc gcgcggaaac tcgccgaccg ggatggcgtg atgtgcgcgc tgaccgaccg
//
LOCUS       DBNGSG010000187        14334 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2270573, whole genome shotgun sequence.
ACCESSION   DBNGSG010000187 DBNGSG010000000
VERSION     DBNGSG010000187.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 14334)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14334)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14334
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2270573"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            485..1675
                     /locus_tag="AC158Q_04910"
     CDS             485..1675
                     /locus_tag="AC158Q_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004521353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase family protein"
                     /protein_id="HIR3339146.1"
                     /translation="MNNGREVVVLSGVRTAIGDYGGALKDMAPTELSAQVIREAVKRS
                     KVDAKEVGACVIGNVIHTEARDMYFSRVAALKGGLPEESTALTVNRLCGSGMQAIVSA
                     SQMIALGDADVAVGGGAESMSRGGYLMPTLRWGQRMSDGSVVDMMVGALTDPFDTVHM
                     GITAEAIAEKWKITREQQDEFSVESHRRAINAIEKGYFKDQILPIEMKTRKGTTVFER
                     DEHPRADASLEGMAKLRAAFKKDGSVTAGNASGINDGAAAVVLMERAAAEKKGLKPMA
                     RLVSYGIAGVDPKIMGIGPVPACKKALEKAGLKVEQMDVIESNEAFAVQALAVSCDLK
                     FDAKKTNPNGGAVGLGHPIGATGAVLTVKALYELQRTGGKYALVTMCIGGGQGIAAVF
                     ERLN"
     gene            complement(1924..2628)
                     /locus_tag="AC158Q_04915"
     CDS             complement(1924..2628)
                     /locus_tag="AC158Q_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR3339147.1"
                     /translation="MRGEANIATVAALIGDPARAGILTALADGRALPAGELAMSAGLS
                     PSAASAHLTKLLEGGLLALEREGRHRYYRLAGPGIADALESLALIAGRPSRAAVVRSP
                     QAEALRYARCCYDHLAGELGVGIAVALEDRGLIASVGPGKRVDVTLAGVAWFEAVFGI
                     DVRKLRPGRHGLACRCLDWTERRHHLAGPLGTRLLQRCFELGWLRRTPRSRAVRLTRK
                     GRDGLQVQLGIGSPAS"
     gene            2708..3460
                     /locus_tag="AC158Q_04920"
     CDS             2708..3460
                     /locus_tag="AC158Q_04920"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR3339148.1"
                     /translation="MFDLTTVIVVSGTFFLAGAVKGVIGLGLPPVSLGLLTIALDLQT
                     AMALLVVPAFVTNLWQSVVGGNAKVIFQRLWPFLVMATVTVWIGVAALKRLNPSLLTA
                     LLGALLVTYSLVSLGGIRLTIPTRHEGWAGPLIGSANGVLTGMTGSSVVPGVMFLQAI
                     GLPRDVLIQAMGTLFAASALALAAALQQADFFTVDHGILSSAAVLPSIAGMIGGRWIR
                     KGLAEQRFRTVFFIALLVLGAYIIANAIRVLR"
     gene            complement(3519..4511)
                     /locus_tag="AC158Q_04925"
     CDS             complement(3519..4511)
                     /locus_tag="AC158Q_04925"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase family protein"
                     /protein_id="HIR3339149.1"
                     /translation="MKAIQITETGGPEVLKYVDLPTPRPGPAEVLVKAHAIGVCMPET
                     LVRKGTYRWMPPLPAIPGIEMSGTVVETGSGVRDLRVGQPVFVSAREFKERGGCYAEY
                     LAAAERSIYALPEGTDLDQAAGLSNYQVAWHVLNTALTGYQYESVLVFAAAGGVGTAL
                     VQLARAAGKRVIGVVSSDERAAFARSQGADEVVDRKRGDVVGQVMALTAGGGVDLVLD
                     PAGGPGMIALFDCLAPFGLLLLYGRLQGPPQGDIDAAIEKAPVRSLAFRQFTMHTLDD
                     MPELRARATRELIRMLSEGAIQVPIYERVPLAEAPRAHRIFESGQVMGKLIMKP"
     gene            complement(4544..5275)
                     /locus_tag="AC158Q_04930"
     CDS             complement(4544..5275)
                     /locus_tag="AC158Q_04930"
                     /inference="COORDINATES: protein motif:HMM:NF018917.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HIR3339150.1"
                     /translation="MDVMSPVTSALYSALRDLREPRVLALGVLPPLAASLVWAGLAWA
                     LADDWARLVANWIASTPWLGWVADWGLASAFIWGSGIAAFALLLPVMLITAVLVTGVV
                     AMPVIVPFVGRRHFPGLEMRRGGGFWASAWTSAVAAALFAVLWMVTLPLWLTGIGALA
                     LPPLLSAWFNRRVLGYDALAEHADARELAVVLHAARGELFLLAFLLSLLLYVPLFNLL
                     VPVLSGLAFTHYGLARLARMRAGSR"
     gene            5346..6992
                     /gene="pgi"
                     /locus_tag="AC158Q_04935"
     CDS             5346..6992
                     /gene="pgi"
                     /locus_tag="AC158Q_04935"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HIR3339151.1"
                     /translation="MSSSRAALPAWQKLVEHRRAVAGLRLRDLFAQDPKRFQAFSLAA
                     PGMLLDYSKQLVTGETMRLLFALARECQVESSIAGMFAGGRVNTTENRAALHVALRAG
                     KPVLLDGADVTRGVKSVLAAMARLTRGIRSGRVRGATGRPFTDAVNIGIGGSDLGPRF
                     ACEALAPYSKARPRLHFVSNVDGAALSSVLERLNPATTLFIIASKTFTTAETLANARS
                     ARAWLSGRLGRKADAGRHFCAVTSAPARAAEFGVPSDRIFPMWDWVGGRYSLWSAIGL
                     PIALAIGAGRFDELRAGARAVDAHFAATPIERNLPVVMGLLEAWNINFLGCATRAVLP
                     YDERLRQLPAYLQQLEMESCGKSVTRDGSPAGHATAPVVWGSAGTDGQHAYFQMLHQG
                     TQAVAMDFIACCRPRHHLRGHHDNLIANFLAQTEALAFGMTADEAAAVMRADGLPEAE
                     IRRLAPHRAFPGNRSSTSILLDELSPRTLGALIALHEHKVFVQSVLWDINAFDQWGVE
                     LGKRLASRILPELGASAPVSGHDASTNALIHHYRKTRGKR"
     gene            6989..7195
                     /locus_tag="AC158Q_04940"
     CDS             6989..7195
                     /locus_tag="AC158Q_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6494 family protein"
                     /protein_id="HIR3339152.1"
                     /translation="MNDEALNMSIRKFLKTVGVNSQLTIEKAVQKAVAEGRLKGNEAF
                     PAAMTLTVGKLAIDVKFDGEIRLE"
     gene            complement(7287..7832)
                     /gene="orn"
                     /locus_tag="AC158Q_04945"
     CDS             complement(7287..7832)
                     /gene="orn"
                     /locus_tag="AC158Q_04945"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HIR3339153.1"
                     /translation="MAQDPNNLIWIDMEMSGLDPDADKVLEVALIVTDSQLNTVAEGP
                     VLVVHQPDAVLDRMDDWNRSTHAKSGLIDRVKSATLDEAGVELRLVAFLAEHVPARTS
                     PMCGNSVHQDRRFLARHMPALEAFFLYRNLDVSTLKELAKRWKPTIMAGLTKHGKHEA
                     LADIRESIEELKYYREHFLKL"
     gene            7924..9162
                     /locus_tag="AC158Q_04950"
     CDS             7924..9162
                     /locus_tag="AC158Q_04950"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR3339154.1"
                     /translation="MTTFGIVFLAALAITTATRLLLASRHLNHVRAHRARVPDAFAAE
                     IDLPAHQKAADYTCAKTGFAMIDLAVGVLVTLALTFGGGLQWLHELSAAWAAEGILRG
                     LVLIALVGALTTLIDLPSDLYRTFVIESRFGFNKTTPWLFFTDLCKSLLVGAALLLPL
                     AALIIWLIRELGAYWWVYAWAATVASGIFVQFIAPTVIAPLFNKFSPMEDPELKSRVE
                     RLLERCGFKVKGLMKMDGSRRSSHGNAYFTGFGKSKRIVFFDTLLQQLDPAEVEAVLA
                     HELGHFKLRHVLKRMALTSALSLGFFWLLGFLMEQAWFFSSLGVASPSPAMALVLFMF
                     VLPPFTFLLQPLGAMLSRRHEFEADQYAAQNASARDLVSALIKLYRDNASTLTPDPLH
                     SAFYDSHPPAVARIARLQKA"
     gene            9277..9618
                     /locus_tag="AC158Q_04955"
     CDS             9277..9618
                     /locus_tag="AC158Q_04955"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HIR3339155.1"
                     /translation="MTNTVKDLASGKCKPCEGGVAPLTEQEVRNLLKQLPGWELSKGR
                     IARTYQFKDYYQTMAFVNAAAWVSHREDHHPDMAVGYNQCRVEYVTHAINGLSQNDFI
                     CAAKLDKLFGL"
     gene            9713..10603
                     /gene="rsgA"
                     /locus_tag="AC158Q_04960"
     CDS             9713..10603
                     /gene="rsgA"
                     /locus_tag="AC158Q_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HIR3339156.1"
                     /translation="MGVTNHATLLKGQIVASFGRRYLVELPDASVVDCVTRGKRGALA
                     CGDRVNVAPSTAGEGVIETREPRSTLLYRSDAVRQKLVAANVTQIVIVVAPLPVFHED
                     LVNRCLAAAEHGGMAPLIALNKSDLPQAPETLAALELYRRMGYRVVALSAKRDLAPLL
                     PLLAGQTSVLVGQSGVGKSTLINALVPAAFVRVAELSAALDSGRHTTTHARLYHLDAA
                     SRIIDSPGMQAFGLHHLDFSELESAFREFRPFLGQCRFRDCRHMTEPGCAITAACERG
                     EIGERRLASYRGLVRERREP"
     gene            complement(10670..11590)
                     /locus_tag="AC158Q_04965"
     CDS             complement(10670..11590)
                     /locus_tag="AC158Q_04965"
                     /inference="COORDINATES: protein motif:HMM:NF005792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family protein"
                     /protein_id="HIR3339157.1"
                     /translation="MKFLALLAALMLEQAWPLRQGNPVHLGFGGYAQVLESRFNGGQR
                     RHGVIAWLLAVLPVAAAVVAVYHVLHGMSPVAAWLWNIAVLYLTMGFRRFSHYFTEIQ
                     QALRAENLATARESLGKWRGESASWLNAAEIARVAIEQGLLASHRHVFGTLFWFVALG
                     PAGAVLYHASAVLAESWGARADAEHIEFGRFASRAFFWIDWVPARLTAASFAVVGDFE
                     DAAYCWRTQAAAWYTQSHGILLAAGGGALGVRLGDVLHQDGEPKFRPVLGTGDEADVD
                     HMEYAAGLIWRALVLWMFLILLVSLAHALG"
     gene            complement(11676..12188)
                     /locus_tag="AC158Q_04970"
     CDS             complement(11676..12188)
                     /locus_tag="AC158Q_04970"
                     /inference="COORDINATES: protein motif:HMM:NF013440.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HIR3339158.1"
                     /translation="MSRAFTKESTDDLVAGELPERPVPAHPNYVTPRGLEHLRERVKA
                     LQEQHEQLAARSDEDSADKQKLREVERDQRYFIAQLDRAVVVDPAGQPRDEVHFGAAV
                     KIIDEDGKAHRFTIVGDDEADVAAGRVSWASPLAKAMMGARVGDTVNWRRPAGDAEVE
                     IVEISYPTKP"
     gene            complement(12413..13552)
                     /locus_tag="AC158Q_04975"
     CDS             complement(12413..13552)
                     /locus_tag="AC158Q_04975"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR3339159.1"
                     /translation="MNNNRHRVGLVLTGGGARAAYQVGVLRAVSEIVPESVRNPFPII
                     CGTSAGAVNAAVLAANGSNFRRGVRMLMTVWKNFRAFHVYRADPVGVFRNTLHWLTAV
                     VAGGGLGRQTPVSLLDNSPLGELLRNRLDFSTIQTAIDAGDLTAVSITCSGYSSGQSV
                     TFYQGVPGLQPWSRARRLGVPMPIAPDHLLASSALPFIFPAVHINREYFGDGSMRQIA
                     PVSPALHLGADRLLIIGVGRQLSQQPQRIKTEGYPSLAQIAGHCLNSIFLDSLEVDLE
                     RLQRINRTLSMMPPDAAAANPVSMHQVDFRLISPSEELERLAMNFKHELPRAIRFLLY
                     GALRRRGSNLLSYLLFEKSFCRALIQLGYKDTMDRKDEIAAFLGY"
     gene            complement(13655..14260)
                     /locus_tag="AC158Q_04980"
     CDS             complement(13655..14260)
                     /locus_tag="AC158Q_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010105459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HIR3339160.1"
                     /translation="MTREFVAAKLRRPAPPPEPRDLHVIALQEGTRVTEAAVLVPLVN
                     RAGRVQVLFTQRTPHLDDHASQISFPGGRVEAGDAGREETALRETEEEIGLARANVAL
                     LGRLPQYEIPSGFRITPVVGWIEPPFTLKPDPFEVAEVFEAPLEHFLHGANYQRREYR
                     LRGRHRHYMAIPYEGRYIWGATAGMLYCLYRMLGRQADATA"
BASE COUNT         2547 a         4797 c         4587 g         2403 t
ORIGIN      
        1 agaaagagag gacgacgctc ctcaaatcga tcatgggcat gaacgtcgcc caagcccaaa
       61 gtatcccaac gtacaacgca tatagcacca aaggcaacgg aacgaacagc agccagaaga
      121 ctttccagag agggcactga cccgcaaacg cgcgggtgag aatggtgccg tcattcctcg
      181 agagagttga ttccattaag acacccaact tttaatggac agcattctag aaatgctaaa
      241 tccgtaaacc ggcatataca actctccaga tggagatatc tgcatatgcg ccggtatttc
      301 tacgcatgta tggattttct tggcgtgctt ggcgtcttgg cggctaatct atggttcttg
      361 ctgtgccgca caatctcgac aggacgaact gcgcggcgta aactcgctct ccctttcaga
      421 ggcacgcggc gattcccggg gacatagcaa tagttatttt gaatcctata gttaggagat
      481 ttccatgaac aacggacgcg aagtagtggt gctgtccggc gtgcggacgg cgatcggcga
      541 ttacggcggc gcgctgaagg acatggcgcc gaccgagctg tcggcgcagg tgatacgcga
      601 ggcggtgaag cgttccaaag tggacgcgaa agaagtcggc gcctgcgtca tcggcaatgt
      661 gattcacacc gaagcgcgtg acatgtactt ctcgcgcgtc gccgcgctga agggcgggct
      721 gccggaggaa tccaccgcgc tcaccgtcaa ccgcctgtgc ggcagcggca tgcaggcgat
      781 cgtttccgct tcacagatga tcgcgctggg cgacgccgac gtcgcggtcg gcggcggcgc
      841 cgagtccatg agccgcggcg gctacctgat gcccacgctg cgctggggcc agcgcatgag
      901 cgacggttcc gtggtggaca tgatggtggg cgcgttgacc gaccccttcg ataccgtgca
      961 catggggatc accgccgagg ccatcgcgga gaaatggaag attacgcggg agcagcagga
     1021 cgagttctcg gtggagagcc accgccgcgc gatcaacgcg atcgagaaag gctatttcaa
     1081 ggaccagatc ctgccgatcg agatgaaaac ccgcaagggc acgaccgtgt tcgagcgcga
     1141 cgagcacccg cgcgcggacg cgagcctgga aggcatggcg aagctgcgcg ccgcgttcaa
     1201 gaaggacggc tcggtcaccg cgggcaacgc ctccggcatc aacgacggcg ctgcggcggt
     1261 ggtattgatg gagcgcgccg cggccgagaa gaagggcctg aagccgatgg cgcggctcgt
     1321 gtcctacggc atcgccggcg tggatccgaa aatcatgggc attggtccgg tgcccgcctg
     1381 caagaaggcg ctggagaagg ccgggttgaa ggtcgagcag atggacgtca tcgaatcgaa
     1441 cgaagcgttc gcggtgcagg cgctcgcggt ttcctgcgac ctgaagttcg acgcgaagaa
     1501 gaccaacccc aacggcggcg cggtggggct cggccacccg atcggcgcga ccggcgccgt
     1561 gctgacggtg aaagcgctct acgaattgca gcgcaccggc ggcaagtacg cgctggtcac
     1621 gatgtgcatc ggcggtgggc aaggcatcgc cgcggtcttc gagaggctca attagccgcc
     1681 aggcctgtcc tgagcaggtc gaagggacgc caagaccgcc aagaaattcg aattgtctct
     1741 gggcgccgcg tgagaaccac gcggcgtttt ctttttcggc gccctccgcc cggacaggat
     1801 agtagcggac gttctgtcac tggccgggtc ctgtcattcc gcctgccgcc tttcgccttc
     1861 atcgagaact cgtaaggccg aggagcgcac cgtcgaggcc gcttgacgag gtgctggcag
     1921 cttctacgac gcgggagagc cgatcccgag ttgcacctgc aggccgtcgc ggcccttccg
     1981 ggtaagccgc accgcacggg atcgaggcgt tcgcctcagc caccccagtt caaagcagcg
     2041 ctggagcaat cgagtaccga gcggcccggc caggtgatgg cggcgctctg tccaatccag
     2101 acatcggcac gccaatccat gccggccggg tctgagcttg cgcacatcga tgccaaacac
     2161 ggcctcgaac cacgccacac cagccagggt gacatccacg cgcttgcccg gtccgaccga
     2221 agcaatcagt ccgcgatctt ccagggccac agcgataccc acgcccagtt cgccagcgag
     2281 atgatcgtag caacatcggg cgtagcgcag cgcttcagcc tgcggcgagc gcacgactgc
     2341 agcgcggctt ggtcgcccgg ctatcagcgc caagctctcc agtgcgtccg cgatccccgg
     2401 cccggccagc cggtagtagc gatggcggcc ctcgcgctcg agcgcaagca ggccaccctc
     2461 cagcaacttg gtcaaatgcg cgctggccgc tgaaggcgaa aggccagcag acatcgccag
     2521 ctcaccggct ggcaacgcgc gaccatccgc gagcgccgtc aggatgccgg cgcgtgccgg
     2581 atcgccaatc agggctgcca cggtcgcgat attggcttcg ccacgcacat ttcatttata
     2641 cgtgaaacat cagtccacgt caaccgccta ggatgggcac tgcaacaaca aaaaccagga
     2701 gagcattttg ttcgatttga ccactgtgat cgtagtcagc ggcacgttct tcctcgcagg
     2761 cgccgtaaag ggcgtgattg gcctcggcct tccgccggtg agcttggggc tgctgaccat
     2821 agcgctcgat ctgcagactg cgatggcgct tctggttgta cctgcctttg tcactaatct
     2881 ctggcagtcg gttgtcggcg gcaacgcgaa ggtcatcttc caaagactct ggccgttcct
     2941 cgtgatggct acagtgacgg tctggatcgg cgtggccgca ctgaagcgcc tgaatccttc
     3001 gttgcttacc gctctgcttg gagcgctgct cgtcacgtat tcgttggtaa gcttgggcgg
     3061 gataagactg acgatcccga cccggcacga aggttgggcc ggccctctga tcgggtcggc
     3121 aaacggcgtg ttgacgggaa tgaccggatc ctcggtcgtg ccaggcgtca tgttcctaca
     3181 agccatcggc ttgccgcggg atgtgctcat tcaggcaatg ggaacgctgt ttgcggcttc
     3241 agcgcttgca ctggcggccg cgttgcagca ggcggacttc tttactgtcg atcatggcat
     3301 cctgtccagc gccgccgtgc tgccgtcgat tgccgggatg atcggagggc gctggatccg
     3361 aaagggtctg gccgaacagc gatttcggac ggtgttcttt atcgccctgc tggtactggg
     3421 cgcctacatc attgccaacg ccattagagt gctccgatga agataaaccg gacaatggat
     3481 tcggtaagga atttgtcggg cttcgccccg acaaattcct aaggtttcat gatcagcttt
     3541 cccatgacct ggccggactc gaagatgcgg tgcgcgcgcg gcgcctcggc caggggcaca
     3601 cgctcgtaga tcggcacctg gatcgcgccc tcgctcagca tccggatcag ctcgcgcgtg
     3661 gcgcgggcgc gcagctccgg catatcgtcc agcgtgtgca tggtgaattg gcggaaagcc
     3721 aggctgcgca ccggcgcctt ctcgatggcg gcgtcgatat cgccttgcgg cggcccctgc
     3781 agccggccgt agagcagaag caggccgaac ggcgccaggc aatcgaacaa cgcgatcatc
     3841 cccgggccgc cggcggggtc gagcaccagg tccacgccgc ccccggcggt caaagccatt
     3901 acctggccga cgacatctcc acgcttgcgg tcgacgacct cgtcggcgcc ctgcgagcgc
     3961 gcgaatgccg cgcgctcatc cgagctcacc acgccgatga cgcgcttgcc ggccgctctt
     4021 gcgagctgca ccagcgccgt tcccacgccg cccgccgccg cgaacaccag gacggattca
     4081 tattgatacc ccgtcagcgc ggtgttgagc acgtgccagg ccacctggta attggacagc
     4141 cccgcggcct ggtcgaggtc ggtcccctcg ggcagggcat agatcgagcg ttccgccgcg
     4201 gccaggtatt cggcgtaaca gccgccccgc tccttgaatt cccgcgcgga tacgaacacc
     4261 ggctgcccga cgcggagatc gcgcaccccg ctccccgtct cgacgacggt gccgctcatc
     4321 tcgatgccgg gtatcgcggg aagcggcggc atccagcgat aggtgccctt gcgcaccagg
     4381 gtttccggca tgcacacgcc gatcgcatgc gccttgacca gcacttccgc cgggccgggc
     4441 cgcggcgtcg gcaggtccac gtacttcagc acctcggggc cgccggtctc ggtgatctgt
     4501 atcgccttca tcgccctcct cgaaccggcc ctcgcggcat tctctatcgg ctgccggccc
     4561 gcatccgggc aagccgcgcc aatccataat gggtaaacgc cagcccggat agcaccggca
     4621 ccagcaggtt gaagagcggc acgtagagca gcaacgacag caggaaggcg agcaggaaaa
     4681 gctccccgcg agcggcgtgc aggacaacgg caagctcgcg ggcgtctgcg tgctcggcga
     4741 gcgcgtcgta tccgagcacg cgccggttga accacgcgga caggagcggc ggcagggcga
     4801 gcgcgccgat gccggtcagc cacaacggca gcgtgaccat ccacagcacg gcgaagaggg
     4861 ccgccgccac cgcggaagtc cacgcgctcg cccagaaacc gccgccgcgg cgcatctcca
     4921 gccccgggaa gtggcgccgc cccacgaacg gcacgatcac cggcatggca acgacgccgg
     4981 tcacgagcac cgccgtgatc agcataaccg gcagcagcag cgcgaacgcc gcgatgccgc
     5041 tgccccagat gaaggccgac gccagtcccc agtcggcgac ccagccgagc cagggcgtcg
     5101 acgcgatcca gttcgccacg agccgcgccc agtcgtcggc cagcgcccac gcaaggccgg
     5161 cccagaccag gctcgccgca agcggcggca gcacgccgag cgcgagcacg cgcggctcgc
     5221 gcaaatcgcg cagcgccgag tagagtgccg acgttaccgg ggacatgacg tccattttaa
     5281 cgggcgccgt tggaatgcgg gtacaatgcc gcacaaccgt accgagtcgc gcaccgccca
     5341 ccggaatgtc ctcctcccgc gccgccctcc ccgcctggca gaaactcgtc gagcaccggc
     5401 gcgctgtcgc cgggctccgc ctgcgcgatc ttttcgcgca ggacccgaag cgcttccagg
     5461 cgttctcgct cgccgccccg ggcatgctgc tcgattactc caagcaactc gtcaccggcg
     5521 agaccatgcg gctgctcttc gcgctcgccc gcgagtgcca ggtcgaaagc tccatcgcag
     5581 gcatgttcgc gggcgggcgc gtcaacacca ccgagaaccg cgcggcgctg cacgtcgccc
     5641 tgcgtgccgg caagccggtc ctgctggacg gcgccgacgt gacccgcggc gtgaagtccg
     5701 tgcttgccgc catggcgcga ttgacgcgcg gtattcgctc gggccgcgtc cggggcgcga
     5761 cgggccgccc cttcacggac gcggtcaaca tcggcatcgg cggctccgat ctcgggccaa
     5821 ggtttgcctg cgaggcgctg gcgccgtact cgaaggcgcg gccgcggctg catttcgtct
     5881 ccaacgtgga cggtgcggcg ctctcgtccg tcctcgagcg gctgaatccg gcgaccacgc
     5941 tgttcatcat tgcatcgaaa acctttacca ccgcggagac gctcgccaac gcccgcagcg
     6001 cccgcgcgtg gctctcgggc cggctcgggc gcaaggcgga tgccggccgg catttctgcg
     6061 cggttacctc cgctcccgcg cgggcggccg aattcggggt gccgtccgac cggatattcc
     6121 cgatgtggga ctgggtgggc gggcgttact cgctgtggtc ggcgatcggg ttgccgatcg
     6181 cgctcgccat cggtgccgga cgcttcgacg agctgcgcgc gggcgcgcgc gcagttgacg
     6241 cgcattttgc ggcgacgccg atcgagcgga acctgccggt ggtcatggga ctgctcgaag
     6301 catggaacat caacttcctg ggatgcgcca cgcgcgcggt gctcccgtac gacgagcggt
     6361 tgcggcagct tccagcctat ctgcagcagc tcgaaatgga atcctgcggc aagtccgtca
     6421 cacgcgatgg cagcccggcc ggccacgcga ccgcgccggt cgtctggggc agcgcgggca
     6481 ccgacggcca gcacgcgtat ttccagatgc tgcaccaggg gacgcaggcg gtggcgatgg
     6541 acttcatcgc ctgctgccgc ccgcgtcacc atctgcgcgg ccaccacgac aacctgatcg
     6601 ccaatttcct cgcgcaaacg gaagcgctgg cgttcggcat gaccgccgat gaggccgccg
     6661 ccgtgatgcg cgcggacggc ctgcctgaag cggagatccg ccgcctggcg ccgcaccgcg
     6721 cgttcccggg caaccgctct tccacctcca tcctgctcga cgaactgtcg ccgcggacgc
     6781 tgggcgcgct catcgcgctc cacgagcaca aagtgttcgt gcaatccgtg ttatgggata
     6841 tcaacgcctt cgaccagtgg ggcgtggagc tcggcaagcg gctggcaagc cgcatcctgc
     6901 ccgaactcgg cgcatccgcg ccggtctcag gtcatgacgc ctcaaccaac gcactcatcc
     6961 atcattacag gaaaacaagg ggaaagcgat gaacgacgaa gcgctcaaca tgagtatccg
     7021 caagttcctg aagaccgttg gcgtcaattc gcagctcacg atcgagaaag cggtgcagaa
     7081 ggccgtggcc gaagggcggc tgaagggcaa cgaggccttc cccgccgcga tgacgctgac
     7141 ggtcggaaag ctcgcgatcg acgtgaaatt cgacggcgag atacggctgg agtagcactc
     7201 cggcgagaga cgccgggcga tagacgaaag acggaaaccg ccgttctgcc cgtctctccc
     7261 ttctcgtcta tcgtctctcc gaacggctag agcttcagga agtgctcgcg gtagtacttc
     7321 aattcctcga tggactcccg gatgtcggcg agcgcctcgt gcttgccgtg cttggtgagg
     7381 ccggccatga tcgtcggctt ccagcgtttc gccagctcct tcaacgtgct cacgtcgagg
     7441 ttgcggtaga gaaaaaacgc ctcgagcgcg ggcatgtgcc gcgcgagaaa gcgccggtcc
     7501 tggtgcacgg aattgccgca catcggggac gtcctggctg gaacgtgctc ggcaagaaac
     7561 gcgaccaggc gcagctcgac cccggcttcg tccagcgtgg cggactttac ccggtcgatc
     7621 agccccgatt tcgcgtgggt ggacctgttc cagtcgtcca tccggtcgag caccgcgtcc
     7681 ggctggtgca ccaccagcac cggcccctcg gccaccgtgt tcaactgcga atcggtaacg
     7741 atcagggcca cctccaggac cttgtcggca tcgggatcga ggccgctcat ctccatgtcg
     7801 atccagatga gattgttggg gtcctgtgcc attattttcg ggagccttgg ggagtttttc
     7861 ggcgataatt gtgtcacagt tcgtgttttg ccgttacagc ggcatgcgca atccccagcc
     7921 ttaatgacca cattcggcat cgtcttcctg gccgcgctcg cgatcaccac cgccacgcgg
     7981 ttgttgctcg ccagccgcca cctgaaccat gtccgcgcgc accgcgcgcg ggtgccggat
     8041 gcattcgcgg ccgagatcga cctccccgcg caccagaaag cggccgacta cacgtgcgcc
     8101 aagaccggtt ttgcgatgat cgacctggcc gtcggtgtgc tggtcacgct ggccctcacc
     8161 ttcggcgggg ggctgcagtg gcttcacgaa ctctccgcgg cgtgggcggc ggagggcatc
     8221 ctgcgcgggc tcgtattgat cgccctggtt ggcgccctga cgacgctcat cgatctgccg
     8281 tcggacctct accggacgtt cgtcatcgag agccgcttcg gattcaacaa gacgacgcca
     8341 tggctgtttt tcaccgacct gtgcaagagc ctgctggtcg gcgcggccct gttgttgccg
     8401 cttgccgcgc tgatcatctg gctgatccgg gaactcggag cctattggtg ggtttacgca
     8461 tgggcggcca cggtggcctc cgggattttc gtgcaattca tcgctccgac cgtcatcgcg
     8521 ccgctgttca acaagttctc gccgatggaa gacccggaac tgaaatcgcg cgtcgagcgc
     8581 ctgctcgagc gctgcggctt caaggtcaag gggctgatga agatggacgg ctcgcgccgc
     8641 agcagccacg gcaacgccta tttcaccggc ttcggcaagt cgaagcgcat cgtgttcttc
     8701 gacacgctgc tccagcagct cgacccggcc gaagtggaag cggtgctggc gcacgaactg
     8761 ggccacttca agctgcggca cgtcctgaaa aggatggcgc tgacttccgc gctgagcctg
     8821 ggattcttct ggctgctggg ttttcttatg gagcaggcgt ggtttttctc gagcctgggc
     8881 gtcgcgtccc cctccccggc catggcgctc gtcctgttca tgttcgtcct gcccccgttc
     8941 actttcctgt tgcagccgct cggcgccatg ctctcgcgca ggcacgagtt cgaggccgac
     9001 cagtacgccg cgcagaacgc ctccgcccgg gacctcgtct cggcgctcat caagctctac
     9061 cgggacaacg cctccacgct cacgcccgat cccctgcact cggcgttcta cgactcccac
     9121 ccgccggccg tcgcgcgcat cgcgcggttg cagaaggcgt gattcgtgat ttgtgattcg
     9181 tgagtcgagc cagccaggcc aaacaggcat agtgatagcc cgaccgggag tcgaatcacg
     9241 acgcacgatt cacgaatcac gcttttggga attcacatga ccaataccgt caaagacctg
     9301 gcatccggca agtgcaaacc gtgcgagggc ggcgttgcgc cgctcacgga gcaggaggtc
     9361 cgcaacctgc tcaagcagtt gcccggctgg gagttgtcca aggggcgcat cgccaggact
     9421 tatcaattca aggactacta ccagacgatg gcgttcgtga acgcggccgc ctgggtctcc
     9481 caccgcgaag accaccaccc cgacatggcg gtcggctaca accagtgccg cgtcgaatac
     9541 gtcacgcacg ccatcaacgg cctgtcccag aacgatttca tttgcgccgc caagctcgac
     9601 aagctgttcg gcctgtaaac cacccgtgaa acgaagtgcg acacgaaccc ggtcgaaccg
     9661 cgaatcacgt accccctcca aaccctcccc cttcccaagg gggagggata gggtgggggt
     9721 cacgaatcac gctacattgc tcaaaggcca gatcgtcgcc agcttcgggc ggcgttacct
     9781 ggtcgaactt cccgacgcct ccgttgtcga ttgcgtcacg cgcggcaaac gcggggcact
     9841 cgcgtgcggt gaccgggtaa acgttgcgcc cagcaccgcc ggcgagggcg tgatcgagac
     9901 gcgggaaccg cgctcgacgc tgctctaccg ctcggacgcc gtgcgccaga agctggtcgc
     9961 ggccaacgtg acgcagatcg tgatcgtggt agcaccgcta ccggtttttc acgaggacct
    10021 ggtgaaccga tgcctggccg ccgccgagca cggcggcatg gccccgctga tcgcgctcaa
    10081 caagtccgac ctgccccagg cgcccgaaac gctggccgcg ctcgaactct accgccggat
    10141 gggataccgc gtggtggcgt tgtcggccaa gcgcgatctc gcgccgctcc tgccgctcct
    10201 ggccggccag accagcgtgc tggtcggcca gtcgggggtg ggcaagtcca ccctcatcaa
    10261 cgccctcgtc ccagcggcgt ttgtgcgcgt cgccgagcta tccgccgcgc tcgactcggg
    10321 gcgccacacc acgacgcacg cgcgcctgta tcacctcgat gccgcgagcc gcatcatcga
    10381 ctcgcccggc atgcaggcct tcgggctgca ccacctggat ttctccgaac tcgagtccgc
    10441 cttccgcgaa ttccggccgt tcctgggaca gtgccgcttc cgcgactgcc ggcacatgac
    10501 cgaacccggc tgcgccataa cggccgcgtg tgaacgcggg gaaatcggcg agagacgcct
    10561 cgcgtcctac cgcgggctcg tccgcgagcg gcgcgagccg tgagtcgtgc gttctgcctt
    10621 tggcaagcga ccgctcagcc gcttccggct taatcgcatc gcgcgccgcc tatccaagcg
    10681 cgtgggcgag gctgacgagc agtatcagga acatccacag gacgagggcg cgccagatga
    10741 gcccggcggc gtactccatg tgatcgacat ccgcctcgtc gccggtaccc aggacgggac
    10801 gaaacttcgg ctcgccatcc tggtgcagca catcgcccag gcgcacaccg agcgccccgc
    10861 cgccggccgc aagcaggatg ccgtgcgact gcgtatacca ggcggccgcc tgggtgcgcc
    10921 agcagtaggc cgcgtcttcg aagtcgccaa cgacggcaaa gctcgcggcg gtcaagcgcg
    10981 ccggtaccca gtcgatccag aaaaaagctc gggaggcaaa acggccgaat tcgatgtgct
    11041 ccgcgtccgc gcgcgcgccc caggactccg cgagcacggc ggatgcatgg tagaggaccg
    11101 cgcccgccgg ccccagcgcc acgaaccaga agagcgtgcc gaatacgtgg cgatgggaag
    11161 caagcagacc ctgctcgatc gccacgcgcg cgatttcggc ggcgttgagc caggacgcgg
    11221 actcgccgcg ccatttcccg agagactcgc gcgccgtcgc gagattctcc gcgcgcagcg
    11281 cctgctggat ttcggtgaag taatggctga accggcggaa gcccatcgtg aggtagagca
    11341 cggcgatgtt ccacagccag gcagcgaccg ggctcatgcc gtgcagcacg tggtaaaccg
    11401 ccacgaccgc cgcggcgacc ggcagcaccg ccagcagcca ggcgatcacg ccatgccggc
    11461 gctgcccgcc gttgaagcgg ctttccagga cttgagcgta gccgccgaac ccgaggtgga
    11521 cggggttgcc ctggcgcaac ggccatgcct gctccagcat cagcgcagcc aacagggcaa
    11581 ggaacttcat gaggtgtgac aggtgatggg tgacgagtga cggtcgagcc ggaagcgtgt
    11641 actgcagtta cgactcacgg atcacgattc accaatcacg gtttcgtggg gtagctgatc
    11701 tcgacaatct cgacttccgc atccccggcc ggtcggcgcc agttgaccgt atcgccgacc
    11761 cgggcgccca tcatcgcctt ggcgagtggc gaagcccagc tcaccctgcc ggcggcgacg
    11821 tcggcttcgt cgtcgccgac gatcgtgaaa cggtgcgcct tgccgtcctc gtcgatgatc
    11881 ttcaccgcgg cgccgaaatg cacctcgtcg cgcggctgcc cggccggatc cacgacgacg
    11941 gcgcgatcga gctgggcgat gaagtaacgc tgatcgcgct cgacctctcg cagtttctgc
    12001 ttgtcggccg agtcttcatc cgaccgcgca gccagttgct cgtgctgctc ctgcagggcc
    12061 ttcacccgct cccgcagatg ctcgagcccc cgcggcgtca cgtaattcgg gtgcgcggga
    12121 accggtcgct cgggcaattc gcccgcgacg aggtcgtcgg tgctttcctt ggtaaaagcc
    12181 cggctcatgg gtattcctaa atcggggcgg agcagaaatc gccggattca ccggcgccgg
    12241 tcatagcctg cgcccggtgc aatccggcga acaaaccggg cgtaccgcgc cccggtttgt
    12301 cctatatata tataggggaa ctccgcgcgt ggcaatagcc ggcgcgtaac cggtgatgat
    12361 gacgaattag agcccaaatg tcttcgatcg ttcacgcctt gctcatgacc cgctagtaac
    12421 ccaggaacgc ggcgatctcg tccttgcggt ccatggtgtc cttgtagccc agctggatca
    12481 gcgcccggca gaaggatttt tcgaacagca ggtacgagag caggttggaa cccctgcgcc
    12541 gcagcgcgcc gtagagcagg aagcggatcg cccgcggcaa ttcatgcttg aagttcatgg
    12601 cgaggcgctc gagttcctcg ctgggagaaa tcaggcggaa gtccacctga tgcatcgaca
    12661 ccgggtttgc ggccgcggcg tcaggtggca tcatggacag agtgcggttg atgcgttgca
    12721 gccgttccag gtccacttcg aggctgtcga ggaaaatgga attcaggcaa tggccggcaa
    12781 tctgcgcgag cgaggggtag ccctcggtct tgatccgctg gggctgctgg ctcaactgac
    12841 gacccacgcc aatgatcagc agccggtccg cgcccaggtg cagcgcggga ctcaccggcg
    12901 cgatctggcg catcgagccg tcgccgaagt actcgcgatt gatatgcacc gccggaaaga
    12961 tgaacggcag cgccgaggac gccagcaggt ggtcgggagc gatcggcatg ggcacgccga
    13021 gacggcgggc gcggctccac ggctgcagtc cgggcacgcc ctggtagaac gtgaccgatt
    13081 gtcccgagct gtagccggag caggtgatgc tcacggcggt caggtcaccc gcgtcgatcg
    13141 cggtctggat cgtggaaaaa tcgagccggt tgcgcagcag ttcgcccagc ggagagttgt
    13201 cgagcagcga taccggcgtc tggcgcccga gccccccgcc ggcgacaacc gccgtgagcc
    13261 aatgcagggt attgcggaac accccgaccg ggtcggcgcg gtagacatgg aaggcgcgga
    13321 aattcttcca caccgtcatc agcatccgca cgccgcgcct gaaattgctg ccgtttgcgg
    13381 cgagcaccgc ggcattgacc gcgccggccg aagtgccgca gatgatcggg aacgggttgc
    13441 gcacgctctc cggcacgatt tctgaaacgg cgcgcagcac gccgacctgg taagcggcgc
    13501 gcgcgccgcc accggtcagc accaggccga ctctgtgccg attattgttc atcttggccg
    13561 cccgaccgga tcccggtcgc ccctgtctcc cggcgtctgc aaccccgccg ttcatgttgc
    13621 cataacgcaa ggccggccgc cgccaggccg cgcttcacgc cgtcgcgtcc gcctgacgcc
    13681 cgagcatccg gtacaggcaa tagagcatgc ccgcggtcgc gccccagata tagcgcccct
    13741 cgtaagggat tgccatgtaa tggcgatgac ggccgcgcag gcggtattcg cggcgctggt
    13801 agttcgcgcc gtgcaggaaa tgctcgagcg gtgcctcgaa cacttccgcc acctcgaacg
    13861 ggtccggttt cagcgtaaac ggcggctcga tccagcccac caccggcgta atgcggaaac
    13921 cggaggggat ctcgtattgc ggcagccggc cgagcagcgc cacattggcg cgcgcaagac
    13981 cgatctcttc ctcggtctca cgcagcgcgg tctcctcccg gcccgcgtcg cccgcctcca
    14041 cgcgtccccc cggaaagctg atctggctgg cgtgatcatc gagatgcggg gtgcgctggg
    14101 tgaacagcac ctgcacgcgc ccggcgcggt tgacgagcgg caccagcacc gcggcctcgg
    14161 tcacccgggt gccttcctgc agcgcgatga cgtgcagatc gcgcggttcg ggcggcggtg
    14221 ccggccgccg cagtttcgcg gcgacgaatt cccgtgtcat cgggaatacc tggccgcgcg
    14281 ttggtgttaa cggtgggtag ttcatgcctg ggcgccaacg tcctgtagtt attg
//
LOCUS       DBNGSG010000188         7731 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4138725, whole genome shotgun sequence.
ACCESSION   DBNGSG010000188 DBNGSG010000000
VERSION     DBNGSG010000188.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7731)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7731)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7731
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4138725"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="AC158Q_04985"
     CDS             complement(<1..505)
                     /locus_tag="AC158Q_04985"
                     /inference="COORDINATES: protein motif:HMM:NF045011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339161.1"
                     /translation="MSEIRSALHRLCEHVGITDGYRDVWGRDHRTTDETRLALLKAMS
                     VIDEVADAEAALRAREEREWRSRLPPVVVRGEQTLPLRWHVCIEESAAYQSLPWTITL
                     ESGEALSGEIRPADFEGVTRRAIDGAGWVKVAFQLRHRLPTGYHRFHVRFPGAVERSM
                     TLIVVPSR"
     gene            657..2345
                     /gene="glgP"
                     /locus_tag="AC158Q_04990"
     CDS             657..2345
                     /gene="glgP"
                     /locus_tag="AC158Q_04990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HIR3339162.1"
                     /translation="MITPYLDRPRIAYFSMEIALASEIPTYSGGLGVLAGDTLRSAAD
                     LRVPMVGVTLASRKGYFRQEIDASGRQVEHADEWDPAQHCVLLRAKVAVRIEGREVWV
                     ASWLYVLESNLARSVPLILLDTDLPENHPQDRTITHHLYGGDSRYRFKQEIVLGVGGL
                     RMLQALGFRIRSYHMNEGHSALLAVELLRVIRATGRRRAHEPGYDAPAVRAMCHFTTH
                     TPVQAGHDQFDWKLVEEVLGELIEFDELKRLAGAAALNMTQLALNLSEYVNGVARRHA
                     EVSRQMFPGYDVHAVTNGVHPATWTSAGFAALFDRHIPGWRHEPQLLTRADQIPDADI
                     WRAHEAAKARLIARVAHESGVALDPALPIIGFARRMTAYKRPELLFSDPERLRAIARR
                     HPFQVVLAGKAHPRDQPGRQAIENIQRSIGSLAGTVRAVYVQDYDMAAALDLVSGSDV
                     WLNTPLRPHEASGTSGMKASFNGVPQLSVLDGWWVEGCIEGVTGWAIGSDQESANGED
                     GTALYDKLEGTVLPLWHGARAQWIAVMKGAIGKNASYFNSHRMLRRYAIEAYLR"
     gene            complement(2387..4522)
                     /gene="glgX"
                     /locus_tag="AC158Q_04995"
     CDS             complement(2387..4522)
                     /gene="glgX"
                     /locus_tag="AC158Q_04995"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120549 - limit dextrin
                     alpha-1,6-maltotetraose-hydrolase activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HIR3339163.1"
                     /translation="MSGPINAVWPGLPYPRGATWDGEGVNFALFSEHATKVELCIFDD
                     KGRRELQRVTLPERTDQVWHCYLPEARPGMLYGYRVHGPYRPEAGHRFNPHKLLLDPY
                     AKSLTGSISWSDANFGYRDGHARADQSQARRDSAPGMPKCRIIDPAFTWGDDRRPAVP
                     WHETVIYELHVKGYTLQHPDVPPALRGTYAGLASAPVIEYLRRLGVTTVELMPVHAFI
                     DDRHLVQKGLRNYWGYNSIGYFAPDNRYAGERPVSEFKSMVKALHSAGIEVVLDVVYN
                     HTGEGDQLGPTLCFRGVDNASYYRLKPGENRYYMDYTGCGNTLNLVHPRVLQLIMDSL
                     RYWVTEMHVDGFRFDLASALARELHEVDRLGAFFDIVRQDPVLAQVKLIAEPWDLGEG
                     GYQVGNFPGGWTEWNDKYRDTMRAYWKGDGGLIGEFAQRLTGSSDLYESSGRRPHASI
                     NFITAHDGFTLHDLVSYDTKHNEVNREDGRDGNDNNLSWNSGAEGETDDAGVKALREQ
                     RKRNFLATLMFSQGVPMITAGDEIGRTQRGNNNAYCQDNEISWVDWNLSPDQRRLFTF
                     AQRVIRLRRDHPVMRRRHFFQGRPIRGTGIKDIMWLKPDGTEMTDSEWQHEHARGLGV
                     LLAGEALAEVDGRGRPVTDGNFLLLFNAHHEEIDFLLPNHTAGLRWLAVLDTARELGL
                     AHGGALEGGRAYVLKGRSLALLREQRTGS"
     gene            complement(4590..5270)
                     /locus_tag="AC158Q_05000"
     CDS             complement(4590..5270)
                     /locus_tag="AC158Q_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR3339164.1"
                     /translation="MESVNIYLEPVRAFLAQVGAFLPRLALALAVLIAGWLAAKLARF
                     TVVKGLRAINFNVLTERAGMDGFLKQGGLERDTTDIFGLLVYWLVIFAALVIACNGLG
                     LTYITDLLGRVVLFVPKVIVALVILAFGAYFAAFIGGTVITYCRNVGIQDADLLGKLA
                     QYAIIGFVVLIALDQINIGGDIIRQSFLIILGGVVLALALAFGIGGQHWAAGLLERWW
                     PRRDNGEQ"
     gene            complement(5305..>7731)
                     /locus_tag="AC158Q_05005"
     CDS             complement(5305..>7731)
                     /locus_tag="AC158Q_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative maltokinase"
                     /protein_id="HIR3339165.1"
                     /translation="QTPDIPETCQWAIFLRNHDELTLEMVTSRERDYMYQTYAADPRA
                     RINLGIRRRLAPLLENDMERIRLMNSLLLSMPGSPIIYYGDEIGMGDNIYLGDRNGVR
                     TPMQWSPDRNAGFSRADPQRLYLPPIMDAIYGYEAVNVEAQSREPNSLLNWMRRLLAV
                     RKTSQAFGRGKLTFLRPGNRKVLAYLREYGEEIVLCVANLGRSAQPVELDLSRFRGRV
                     PVEMLGRTPFPPIGELPYLLTLPLCGFYWFRLAPAGEVDAPSWHEERMGRDDLPVMVL
                     FDGWTSFFRNRVVPWRIGMAVKTREQLETDVLARHVEAQRWYAAKGTPVKRANLADWA
                     LWEAGRSNCMIALLEVEGGPETATYFAPLTLAWEDRDEERVRALGPATIAKVRQQAQV
                     GVIADALADEAFCHAVVAAIGEGREIAMAQGKLRFAPTNAFAAIAGEGYAELPVGRPK
                     TLSSNTAVTFGDRLFFKGYRRIRAGINPEFEVGRFLTERTNFASCVPVAGAIEYTAGD
                     GTTMTLGLLQAYASNQGDGWTYTLDYLERFFDQNRAATATPAEDAHGVYLALVRTLGQ
                     RTGELHRAFAASSGDPGFDPEPVAPEEFSAWKAGIQAEAAETLELLERNLKQLVLPAR
                     DAAHGLLAQRAVLAARVEQCAPGAGPALKMRYHGDYHLGQVLLSKQDFLIVDFEGEPT
                     RPFSERRRKHSPLRDVAGMARSFDYARWTALRNVAQSPEEHERFSALAGAWHDASLET
                     FLAAYAGATKDGGLYASFADARALLALFQLEKALYELRYEINNRPGWVQVPLQGILAL
                     INADRPAQTA"
BASE COUNT         1265 a         2686 c         2503 g         1277 t
ORIGIN      
        1 agcgcgatgg gaccacaatc agggtcatgg atcgctcaac cgcgcctgga aaccgcacgt
       61 gaaaccggtg atagccggtc ggcagccggt gacgcaattg aaaagccacc ttgacccagc
      121 ccgcgccgtc gatcgcccgg cgcgtcacgc cctcgaaatc ggccggacgg atttccccgc
      181 tcagcgcctc acccgactca agcgtaatcg tccacggcag cgactggtat gcggccgatt
      241 cctcgatgca gacatgccac cgcagcggca gtgtctgctc gccgcggacc acgaccggcg
      301 gcagacggct gcgccattcg cgttcctcgc gcgcccgcaa cgccgcctcc gcgtcggcga
      361 cttcgtcgat cacgctcatc gccttgagca aagccaaccg ggtctcgtcg gtcgtccggt
      421 ggtcgcggcc ccagacatcg cggtagccgt ccgtgattcc gacgtgctcg caaagccggt
      481 gaagcgcgct gcggatttcg ctcatccggt cacgcctgcc atcgggaagg gttattcgtt
      541 gaacggaata tcacgcgcgg ttttcggcgc ctgcaccggt tcaaagcacg ccggctccgt
      601 ccgcggtact ggcgcgccat tgctggtaag ctaacaggtg tgatggaatt cgagccgtga
      661 tcaccccgta cctcgaccgg ccgcgcattg cctacttctc gatggaaatt gcgctcgcct
      721 ccgaaattcc gacctacagc ggcgggctgg gcgtcctggc cggcgacacg ctgcgttccg
      781 ccgcggacct gcgcgtgccc atggtcgggg tgacgctcgc cagccgcaag ggctatttcc
      841 ggcaggagat cgacgcatcc ggccgccagg tcgagcatgc ggacgagtgg gatcccgcgc
      901 agcactgcgt gctgctgcgg gccaaggttg cggtcaggat cgagggccgc gaagtctggg
      961 tcgcgagctg gctctatgtc ctcgagagca accttgcgcg cagcgtgccc ctgatcctgc
     1021 tcgacaccga tctgcccgag aaccatcccc aggaccgcac gatcacgcat catctctacg
     1081 gcggggactc ccgataccgc ttcaagcagg agatcgtgct cggcgtcggc ggattgcgca
     1141 tgctgcaggc gctcggcttc cgtatccgca gctaccatat gaacgagggc cactcggcgc
     1201 tgctcgcggt ggaactgctg cgcgtgattc gcgcaacggg ccggcgccgc gcccacgaac
     1261 cgggctacga cgcgccggcg gtgagggcga tgtgtcattt cactacccac accccggtgc
     1321 aggctggcca tgaccagttc gactggaagc tggtcgaaga ggtcctcggc gagttgatcg
     1381 agttcgacga actcaagcgc cttgccggcg ccgccgcgct caacatgacc cagctggctc
     1441 tcaacctcag cgagtacgtg aacggcgtcg cgcggcggca tgcggaggtg tcacggcaga
     1501 tgttcccggg gtatgacgtg cacgcggtca ccaatggcgt gcaccccgcc acgtggacct
     1561 cggcgggatt cgccgccctc ttcgaccggc acatcccggg ctggcgccac gagccccagc
     1621 tgctcactcg cgccgaccag attccggacg cggacatctg gcgcgctcac gaggccgcca
     1681 aggcccgcct catcgcgcgg gtcgcgcacg aatccggagt cgcgctcgac cccgcgctgc
     1741 cgatcatcgg gtttgcgcgg cgcatgacgg cctacaagcg tcccgaactg ctcttctccg
     1801 atcccgagcg gctgcgcgcc atcgcgcgcc gccatccgtt tcaggtcgtg ctcgcgggca
     1861 aggcgcaccc gcgcgaccaa cccggcaggc aggcgatcga gaacatacag cggagcatcg
     1921 gctcgcttgc aggcacggtg cgtgccgtct atgtgcagga ctacgacatg gccgcggcgc
     1981 tcgatctcgt ctccggatcg gatgtctggc tgaacacacc gctgcgcccg cacgaggcct
     2041 ccggaacgag cggcatgaaa gccagcttca acggcgtgcc ccagctctcg gttctcgacg
     2101 gctggtgggt cgaaggatgc atcgagggtg tcaccggctg ggcgatcggc agcgaccagg
     2161 aaagcgcaaa cggcgaggac gggacggcgc tctacgacaa gctcgagggc accgtgctgc
     2221 ctttgtggca cggtgcccgc gcgcagtgga tcgcggtcat gaagggcgcc atcggcaaga
     2281 acgcgtccta cttcaacagc caccgcatgc tgcgccgtta cgcgatcgag gcctacctcc
     2341 gctaggacgg gcgcgggcct gcggggggca cggggcggcc cggccatcac gatccggtcc
     2401 tctgttcccg cagcagcgcg agcgagcgcc ccttcaggac gtaggcccgc cccccttcca
     2461 atgcgccgcc gtgggccagg cccagctcgc gcgccgtgtc gaggacggcc agccaccgca
     2521 gccccgcggt atggttgggc agcaagaagt cgatttcttc gtgatgcgca ttgaacaaca
     2581 gcaggaaatt gccgtcggtc accgggcggc cccgcccgtc cacttcggcg agagcctcgc
     2641 cggcgagcag cacccccagc ccccgcgcat gctcgtgttg ccattcggaa tcggtcatct
     2701 cggtgccgtc gggcttcaac cacatgatgt ccttgatgcc cgtgccgcgt atgggccggc
     2761 cctggaagaa gtggcggcgc cgcatcaccg gatggtcgcg ccgcagccgg atcacccgct
     2821 gcgcgaacgt aaacagccgc cgctggtccg gggagaggtt ccagtccacc cacgagatct
     2881 cgttgtcctg gcaataggcg ttgttgttgc cgcgctgggt tcggccgatc tcgtcgcccg
     2941 cggtaatcat cggcacgccc tgcgagaaca tgagggtcgc aaggaaattg cgcttgcgct
     3001 gctcgcgcag ggccttgact cccgcgtcgt ccgtctcgcc ctccgctccg ctgttccatg
     3061 acagattgtt atcgttcccg tcgcggccgt cctcgcggtt cacttcgttg tgcttggtgt
     3121 cgtagctcac gaggtcgtgc agagtgaacc cgtcgtgcgc cgtgatgaaa ttgatgctcg
     3181 cgtgcggccg gcggccgctg ctctcgtaga gatcgctcga acccgtcagc cgctgcgcga
     3241 actcgccgat cagcccgccg tcgcccttcc aatacgcgcg catcgtgtcg cggtacttgt
     3301 cgttccactc ggtccagccg cccgggaaat tgccgacctg gtagccaccc tcgcccaggt
     3361 cccagggctc ggcgatcagc ttcacctgcg ccagcacggg atcctggcgc acgatgtcga
     3421 agaacgcccc cagtcgatcc acctcgtgca gttcgcgcgc gagcgcagac gcaaggtcga
     3481 agcgaaaccc gtccacgtgc atttccgtca cccagtagcg cagcgagtcc atgatgagct
     3541 gcagcacccg cggatgcacc aggttgagcg tgttgccgca gcccgtgtag tccatgtaat
     3601 agcggttttc gcccggcttc agccggtagt aggaggcgtt gtccacgccg cgaaagcaca
     3661 gcgtcggccc cagctggtcg ccctcgccgg tgtggttgta gaccacgtcg agcacgacct
     3721 cgatgcccgc cgagtgcagc gctttcacca tcgacttgaa ttcggagacg gggcgctcgc
     3781 ccgcgtagcg attatcggga gcgaagtacc cgatcgagtt gtagccccag tagttgcgca
     3841 gccccttctg caccaggtgg cggtcatcga tgaacgcgtg caccggcatc agctccaccg
     3901 ttgttacgcc caggcgcctc aggtactcga tcaccggcgc cgacgccagg ccggcgtacg
     3961 tgccgcgcag cgccggcggg acatcggggt gctgcagcgt gtagccttta acgtgcagct
     4021 cgtagatcac cgtttcatgc cagggaacag ccggccgccg gtcgtcgccc caggtgaacg
     4081 ccggatcgat gatccggcac ttcggcatgc cgggggccga gtcgcggcga gcctgcgact
     4141 ggtcggcgcg cgcgtgcccg tcgcggtagc cgaaattggc gtcgctccag ctgatcgagc
     4201 ccgtcagtga tttcgcgtac gggtcgagca gcagcttgtg gggattgaag cggtgacccg
     4261 cctccggccg gtacggcccg tggacacggt agccgtagag catgccgggc cgcgcctccg
     4321 gcaggtagca atgccagacc tggtcggtgc gctcggggag agtcacacgc tgcaattcgc
     4381 gccggccctt gtcgtcgaag atgcagagct ccaccttcgt tgcgtgctcg gagaatagcg
     4441 cgaaattgac cccctcgccg tcccacgtcg cgccgcgcgg ataaggcagg cccggccaga
     4501 ccgcgttgat cggcccgctc acggcgccct acttctccga tgcgggcgcc gcgcgccccg
     4561 gaaacccgcg ccgccgcatc gccctccccc tactgttcac cgttgtcgcg gcgcggccac
     4621 cagcgctcga gcagccctgc cgcccaatgc tgcccgccga tgccgaaggc aagcgccagg
     4681 gccagcacca cgccgcccag tatgatgagg aagctctggc ggatgatgtc gccgccgatg
     4741 ttgatctggt cgagtgcgat cagtaccacg aagccgatga tcgcgtactg ggcaagcttc
     4801 ccgagcaggt cggcgtcctg gattcccacg ttgcggcaat aggtgatgac ggtgccgccg
     4861 ataaacgctg caaaataggc gccgaacgcc aggatcacga gggcaacgat caccttgggc
     4921 acgaacagca ccacgcgccc gagcaaatcg gtgatgtagg taaggccgag accgttgcac
     4981 gcgatcacca gtgccgcgaa tatcaccagc cagtacacga gcaacccgaa gatatcggtc
     5041 gtgtcgcgct ccaggccgcc ctgcttcagg aagccgtcca tgcccgcgcg ctcggtcagc
     5101 acattgaagt tgatcgcgcg cagccccttc accaccgtga atcgagcgag cttcgcggcc
     5161 agccagccgg caatgagcac cgccagcgcc agcgcgagcc gcggcaggaa tgccccgacc
     5221 tgcgcgagaa atgcccgcac cggttccaga tagatgttga cgctttccat attcgcctcc
     5281 tcttcgcggt gagaccagga atctctaagc cgtttgcgcc ggccggtcgg cgttgatcag
     5341 cgccaggatg ccctgcagcg gcacctggac ccagcccggc cggttgttga tctcgtaacg
     5401 cagttcgtag agcgcctttt ccagctggaa cagcgcaagc aacgcgcgcg cgtcggcgaa
     5461 cgacgcataa aggccgccgt ccttcgttgc gccggcgtag gccgcgagga aggtctcgag
     5521 cgacgcatca tgccaggcgc cggccagcgc ggagaagcgt tcgtgctcct cgggcgattg
     5581 cgcgacgttg cgcagcgccg tccagcgcgc gtagtcgaac gaccgcgcca tcccggccac
     5641 gtcgcgcaac ggcgaatgct tgcgccggcg ctcggagaat ggccgcgtgg gctcgccctc
     5701 gaaatcgacg atcaggaaat cctgcttcga cagcagcacc tgtcccaggt ggtagtcccc
     5761 gtgataacgc atcttgagcg ccggccctgc cccgggcgcg cattgctcga cccgggcggc
     5821 cagcacggcg cgctgcgcca gcagcccgtg cgcggcgtcc cgcgcgggca atacgagctg
     5881 cttgagattg cgctcgagca gttcgagcgt ctcggctgcc tccgcctgga tcccagcctt
     5941 ccaggcggag aattcctccg gcgcgaccgg ctccggatcg aaaccagggt cgccggagga
     6001 tgccgcgaac gcgcggtgca gctcgcccgt tctctgcccc agtgtccgga cgagcgccag
     6061 gtagacgccg tgcgcgtcct cggccggcgt cgcggtggcg gcccggttct ggtcgaaaaa
     6121 tcgctccagg taatccaggg tgtaggtcca gccatcgccc tggttggagg cataggcctg
     6181 gagcagccca agcgtcatcg tcgtgccgtc gccagccgta tattcgatcg ctcccgcgac
     6241 cggcacgcag ctcgcgaaat tcgtccgctc ggtgagaaag cggccgacct cgaattccgg
     6301 gttgatcccg gcgcggatgc ggcggtagcc cttgaagaac aggcggtcgc cgaacgtcac
     6361 cgccgtgttg ctgctcaggg tcttgggcct gccgaccggc aattccgcgt agccctcgcc
     6421 ggcaatcgcg gcgaatgcat tggtcggcgc gaagcgcagc ttgccctgcg ccatggcaat
     6481 ctcgcgcccc tcgccgatcg ccgcgacgac ggcgtggcag aacgcttcgt ccgccagcgc
     6541 atcggcgatc acgcccacct gcgcctgctg ccgcaccttc gcgatcgtcg ccggcccgag
     6601 cgcgcgcacg cgctcctcgt cccgatcctc ccacgcgagc gtcagcggcg cgaaataggt
     6661 cgccgtttcg gggccgccct cgacctccag cagcgcgatc atgcagttcg accggccggc
     6721 ttcccacaat gcccagtcgg cgaggttcgc gcgcttcacc ggggtgccct tggccgcgta
     6781 ccagcgttgc gcctcgacgt ggcgcgccag cacgtccgtt tcgagttgtt cgcgcgtctt
     6841 caccgccatg ccgatgcgcc agggcacgac gcgattgcgg aaaaagctgg tccagccgtc
     6901 gaacagcacc atcacgggca gatcgtcgcg ccccatccgc tcctcgtgcc agctgggcgc
     6961 gtccacctcg ccggccggcg ccagccggaa ccagtagaag ccgcacagcg gcagggtcag
     7021 caggtagggc agctcgccga tcggcggaaa cggcgtgcgg cccagcatct cgaccggaac
     7081 ccggccgcga aaccgggaga ggtcgagttc caccggctgt gccgagcggc cgaggttggc
     7141 gacgcacaga acgatctcct cgccatactc gcgcagatac gcgagaacct tgcggttgcc
     7201 gggcctgagg aaggtgagct tgccgcgccc gaacgcctgg ctggtcttgc gcacggcgag
     7261 cagccgccgc atccagttga gcagtgaatt gggctcgcgc gactgcgctt cgacgttgac
     7321 cgcctcgtag ccgtagatgg cgtccatgat cggcggcaga tacagtcgct gcggatcggc
     7381 ccgcgagaac cccgcgttgc ggtcgggact ccactgcatc ggcgtgcgca cgccgttgcg
     7441 gtcgccgagg tagatgttgt cgcccatgcc gatctcgtcg ccgtaataga tgatcggcga
     7501 gccgggcatg ctgagcagca ggctgttcat cagccggatg cgttccatgt cgttttccag
     7561 tagcggcgcg agccggcggc ggatgccaag attgatgcgc gcgcgcgggt cggccgcgta
     7621 ggtctggtac atgtaatcgc gctcgcggct ggtcaccatt tccagcgtca gctcgtcgtg
     7681 gttcctgaga aaaatcgccc actggcaggt ctcgggtata tccggggtct g
//
LOCUS       DBNGSG010000189         2480 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_912473, whole genome shotgun sequence.
ACCESSION   DBNGSG010000189 DBNGSG010000000
VERSION     DBNGSG010000189.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_912473"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1074)
                     /locus_tag="AC158Q_05010"
     CDS             complement(1..1074)
                     /locus_tag="AC158Q_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339166.1"
                     /translation="MPYKILFMGASYGSLLASKLIPGGHTMHFICLPAEAELINKEGI
                     RVRLPVKGREGLVEINSQKLKGKLSAGGPAGVNPADYDLICLAMMEPQYRSPGVRELL
                     DAVAKSKVPCMSIMNMPPLPYLKRIPGLNTDGLRAAFADATVWDSFDPKFITLCSPDP
                     QAFRPPDEKVNVLQVRLPTNFKAAKFDSDQHTAILRSLEKDVEAARFDDGTGAKLELP
                     VKLKVHDSIWVPLAKWPMLMAGNYRCVTKDGMRPIKDTVHGDLALSKSVYDWVVKLCV
                     SLGASEKDMVPFEKYANAALSLLSPSSAARALYGGAPNIERVDRLVQAIAKQKGINNA
                     AIDEVVGLVDKQLELNRAKAKAA"
     gene            complement(1359..1742)
                     /locus_tag="AC158Q_05015"
     CDS             complement(1359..1742)
                     /locus_tag="AC158Q_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HIR3339167.1"
                     /translation="MHDGDTLTVLVDRRQVKVRLTDIDAPELGQPFGKNSKQSLSDLC
                     FGKLATLDVRGRDRYKRTIAQVTCEGRDANAEQVRRGYAWTYTRYAPAASPLYAMQNE
                     ARAAHRGLWADPKSMPPWEWRRNGN"
     gene            complement(2170..>2480)
                     /locus_tag="AC158Q_05020"
     CDS             complement(2170..>2480)
                     /locus_tag="AC158Q_05020"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HIR3339168.1"
                     /translation="LDLSQTATINDSLKLAKAVLSEIGGINTLHKPHVEQAGFAVLKA
                     PDIPSILVETAFISNPEEERKLKNAAYQEKMAEAVFNGIKAYFSKNPPLARDRLVQNP
                     "
BASE COUNT          458 a          763 c          759 g          500 t
ORIGIN      
        1 tcaggcggct ttcgccttcg cccgattgag ttccagctgc ttgtccacca ggccgacgac
       61 ctcgtcgatc gccgcgttgt tgatgccttt ctgcttcgcg atcgcctgca ccagccggtc
      121 cacgcgctcg atgttgggcg cgccgccgta gagcgcccgc gccgcggagg agggcgagag
      181 cagcgacagc gcggcgttgg cgtacttctc gaacggcacc atgtccttct ccgaggcgcc
      241 gagcgacacg cacagcttca ccacccagtc gtagacgctc ttcgacagcg ccaggtcgcc
      301 gtgcaccgtg tccttgatcg ggcgcatgcc gtccttggtc acgcagcggt agttgcccgc
      361 catcagcatc ggccacttcg cgagcggcac ccagatcgag tcgtgcacct tgagcttcac
      421 cggcagctcg agcttcgcgc ccgtgccgtc gtcgaagcgc gccgcctcga cgtccttctc
      481 gaggctgcgc aggatcgcgg tgtgctggtc ggagtcgaac ttcgccgcct tgaagttggt
      541 cggcagccgc acctgcagca cgttgacctt ctcgtcgggc gggcggaacg cctgcggatc
      601 cgggctgcac agcgtgatga acttcggatc gaagctgtcc cacaccgtcg cgtcggcgaa
      661 cgccgcgcgc aggccgtcgg tattgaggcc ggggatgcgc ttgaggtagg gcagcggcgg
      721 catgttcatg atggacatgc acggcacctt cgatttcgcc accgcgtcga gcagctcgcg
      781 cacgccgggg gagcggtact gcggctccat catggcgagg cagatgaggt cgtagtcggc
      841 gggattgact cccgccgggc cgccggcgga gagcttgccc ttcagcttct gcgagttgat
      901 ctcgaccagc ccttcgcggc ccttcaccgg cagccgcacg cggatgcctt ccttgttgat
      961 cagctccgcc tcggccggca ggcagatgaa atgcatcgtg tgcccgcccg gaatcagctt
     1021 cgaggccagc agcgagccat acgacgcgcc catgaaaagt atcttgtacg gcatgacgct
     1081 cctcctgttt gtccaaatgg atgcggggac tgaatctcgg attgtggaac ccgttcccgc
     1141 tatctacttg cccgggactg tattccatta ccccgctcgg gtgcaatgct gcggagcaac
     1201 aaggcttccc cagggaggtc gggagaattg acaggcgtct caggtaaagc gtctcagatg
     1261 aatcgtctca ggtaaggcgt cgcagttgag gcgctctatc cgagacgcag tatccgcgac
     1321 gtagtacctg aaacgcagta tctgcagcgc tcttccatct agttgccatt acgccgccat
     1381 tcccacggcg gcatggattt tggatcggcc cacaggccgc ggtgagcggc gcgggcctcg
     1441 ttctgcatgg cgtagagcgg cgaggcggcg ggcgcgtagc gcgtgtaggt ccacgcgtag
     1501 ccgcgccgca cctgctcggc attggcgtcg cggccttcgc aggtgacctg ggcgatggtg
     1561 cgtttgtagc ggtcccgccc gcgcacgtcg agcgtggcga gcttgccgaa gcacaggtcg
     1621 gacagggact gcttcgagtt cttgccgaac ggctggccca gctcgggcgc gtcgatgtcc
     1681 gtcagccgca ccttcacctg cctgcggtcc accagcaccg tcagcgtgtc cccgtcgtgc
     1741 accgaaacca cccgcgcggg cccgcccgcg tgaacgggaa acgctgctgt aagaaggaat
     1801 agacaggatg cgaagaatcg gaagaggctc gggccaaagt aaatcaagcg agagccctgc
     1861 tgcggttaaa gccggcgggt aagcaatagt taatagcaaa tttccttcag ctgaaacccg
     1921 tcaaaatcga aatagacatg attcactcag ctcaaaaccc gcccaaacca ctaatagaca
     1981 ggattatcaa aattacgaaa ttcaaaaccc gccaatacca taaatagaca ggattaacag
     2041 gatttacagg attagaacga ataccgcttc aaaaccgaag tttttgtttg aatcttgtta
     2101 atcttgttaa tcctgtccat tgatggggtt aacgggtttt aacattcttc gaaaagtgaa
     2161 tcctgtccgt tacgggtttt gaaccagccg gtcgcgcgcg agcggcggat tcttggagaa
     2221 atacgccttg atcccgttga acaccgcctc cgccatcttt tcctggtagg cggcgttctt
     2281 cagctttctt tcctcctcgg ggttgctgat gaaggcggtc tccaccagga tcgagggtat
     2341 gtcgggcgct ttcagcaccg cgaagccggc ctgctcgacg tgcggcttgt gcagggtatt
     2401 gatcccgccg atctccgaga gcacggcctt ggcgagcttc aggctgtcgt tgatggtcgc
     2461 ggtctgcgag agatcgagca
//
LOCUS       DBNGSG010000190         6910 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3459270, whole genome shotgun sequence.
ACCESSION   DBNGSG010000190 DBNGSG010000000
VERSION     DBNGSG010000190.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6910)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6910)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6910
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3459270"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1224)
                     /gene="leuS"
                     /locus_tag="AC158Q_05025"
     CDS             complement(<1..1224)
                     /gene="leuS"
                     /locus_tag="AC158Q_05025"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012363082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HIR3339169.1"
                     /translation="MQQKYNPHVVERDAQQHWENTGTFRAQETPGKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLTRYHRMKGCNVMQPMGWDAFGLPAENAAMANGVPPAQWTY
                     DNIAYMKKQLKSLGFAIDWARELATCRPEYYRWNQWLFLRMLEKGMAYKKTQVVNWDP
                     VDQTVLANEQVIDGRGWRTGAVIEKREIPGYYLAITNYADELLAALDTLPGWPERVKT
                     MQANWIGKSYGVRFAFPYEVDGRAEKLWVFTTRADTIMGVTFCAVAAEHPLAARAAKS
                     DPKLAAFVEECKRGSVMEADLATVEKKGMPAGISVIHPLTGEAVPVWVGNYVLMGYGE
                     GAVMAVPAHDERDFHFARKYGLPIRQVIDVEGASAFTTDNWQPWYESKDGACIHSGKY
                     DGLRYNAAVDAIAGDL"
     gene            1309..3588
                     /locus_tag="AC158Q_05030"
     CDS             1309..3588
                     /locus_tag="AC158Q_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HIR3339170.1"
                     /translation="MSPDFLSGLNERQLEAVTLPHQSALILAGAGSGKTRVLTTRVAW
                     LIQTGQASPHGLVAVTFTNKAAKEMLSRISAMLPINTRGMWVGTFHGLCNRLLRAHFR
                     EAGLPQLFQILDSQDQLSLIKRLLKGLNIDEERFPPRQVQWFVNSAKDEGLRAAQVEA
                     GDEFARRMRDIYAAYDEQCNREGVADFAELLLRSFELLSTHASLRDHYRARFRHVLVD
                     EFQDTNRLQYAWLKLLAGGKDAAQGVDNAIFAVGDDDQSIYRFRGASAANMQELQRDF
                     AIERVIKLEQNYRSQGHILDAANALIGHNRRRLGKNLWTAESKGEPLRVFDAATDFEE
                     AAFVVDEARALRAEGVALAQIAVLYRSNAQSRVLEHALFTAGIPYRVYGGLRFFERQE
                     VKHALAYLRLVAGSADDGALARVINFPTRGIGARSLEQLAEAAKERGASLWQAALAKS
                     QVTSHKSQGDRSEAGNSSLITHHSSLPKGIPAFVALIGQMREATAGLPLPEIIGLVIE
                     ASGLNAHYRSEREGADRLENLAELINAAAAFVAERDVQPAGEAAGVDEPDELTAFLAH
                     AALEAGEHQALAGADALQLMTVHSAKGLEFHSVFVSGLEEGLFPHENSLTEADGIEEE
                     RRLMYVALTRARRRLYLSFAQSRMLHGQTRYGIASRFFREIPENLLRHIRPVGARDSS
                     PGRSTARSASHPPPIAHHPSLAPDSPWRIGQSVVHATFGAGVIVNAEGRGPDARVQVN
                     FRNGGLKWLMLEYAKLIPA"
     gene            complement(3645..4427)
                     /locus_tag="AC158Q_05035"
     CDS             complement(3645..4427)
                     /locus_tag="AC158Q_05035"
                     /inference="COORDINATES: protein motif:HMM:NF041043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HIR3339171.1"
                     /translation="MEVRVVAAGKGWQWIVDGFALFRKSPAMWIALTVALALIWVVSL
                     VVPLLGPLLFTLLTPVFFAGLMTGCRAVENGEPLEIQHLFAGFKQQAAPLVTIGGVYL
                     VGTVIVMGIVLIAAGGSMLPTVLSKGAGADIETLRAALRSLAIALAIGAAAYLPLIML
                     IWFAPLLVIFGEMAPLAAMKLSFSACLRNTAPFLVYGLAILVMWLVMSLPAAGGVVGS
                     VLVAALLIASIPVLICSIYASYKDVFTAPRGASTTGGNPLLR"
     gene            complement(4526..5476)
                     /locus_tag="AC158Q_05040"
     CDS             complement(4526..5476)
                     /locus_tag="AC158Q_05040"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019254414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HIR3339172.1"
                     /translation="MSVFTTVTREQLGNWLRNYSIGTLSAFEGVAAGIENTNYFVTTS
                     QGRYVLTLFEKLKAHELPFYLELQDHLAGKGVPCPRPVANHAGGFLGELNGKPAALAS
                     FLTGKDVAAPDAGQCAQAGAMLAAIHVAGASYSGRMDNPRGPRWWESVMPAVVPFLGA
                     ADAKLLEEEVRFQLHDRMHDLPRGAIHADFFRDNVLFENGRISGVIDFYFACTGALLY
                     DVAIAANDWCVDGSGALDATRAAALLEAYARARPFTAAERGAWPVMLRAGALRFWVSR
                     LYDFYLPRPGELTHAKDPGHFRGILAAHIAAGDRLPQLAP"
     gene            complement(5561..5914)
                     /locus_tag="AC158Q_05045"
     CDS             complement(5561..5914)
                     /locus_tag="AC158Q_05045"
                     /inference="COORDINATES: protein motif:HMM:NF022639.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2782 domain-containing protein"
                     /protein_id="HIR3339173.1"
                     /translation="MRFPHLLLILAALLLAAALPVAAQQGKPPVLQPVPEPPPPPPGY
                     EFDSAIEPQVTILKRGTDTVEEYRIGGRLYMVKVTPPHGKPYYMIDHRGDGTFTRHDN
                     YDSGIRPPMWVIFQF"
     gene            6280..>6910
                     /locus_tag="AC158Q_05050"
     CDS             6280..>6910
                     /locus_tag="AC158Q_05050"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HIR3339174.1"
                     /translation="MKTLLLVDGSSYLYRAFHALARANLRNRKGEPTGAVYGVINMLR
                     RLLKDIPVDYAACVFDAKGRTFRDDLYADYKANRPAMPDDLVAQLGPLKETIAAMGWP
                     LLEIEGVEADDVIGTLATQAGHQGLRVIIASGDKDLAQLVNPVITMENADPRTGGREI
                     LDEAGVAKKFGVPPGRIIDYLTLIGDSVDNVPGVADVGPVTAVKWLAEYG"
BASE COUNT         1224 a         2250 c         2252 g         1184 t
ORIGIN      
        1 caggtcgccg gcgatcgcgt cgacggcggc gttgtagcgc aggccgtcgt attttccgga
       61 gtggatgcag gccccgtcct tgctctcgta ccagggttgc cagttgtcgg tggtgaacgc
      121 gctggcgccc tcgacgtcga tgacctgcct gatcggcagc ccgtactttc tggcgaaatg
      181 gaaatcccgc tcgtcatggg cgggaacggc catgacggcg ccctcgccgt agcccatcag
      241 cacatagttg ccgacccaca ccgggaccgc ctcgcccgtg agcgggtgaa taacgctgat
      301 gcctgccggc atgcctttct tttcgacggt ggcgagatcg gcctccatca cggagccgcg
      361 cttgcattcc tcgacgaacg cggcgagctt cgggtcgctc ttcgccgccc gcgccgcgag
      421 cgggtgttcg gcggctaccg cgcagaacgt gacgcccatg atggtgtcgg cgcgcgtggt
      481 gaacacccac agtttttcgg ccctgccgtc cacctcgtag ggaaaagcga agcgcacgcc
      541 gtagctcttg ccaatccagt tggcctgcat ggtcttcacg cgctcgggcc agccgggaag
      601 cgtgtcgagc gcggcgagca gctcgtccgc gtaattcgtg atcgccaggt agtagccggg
      661 gatctcgcgc ttctcgatca cggcaccggt gcgccagccg cggccgtcga tcacctgctc
      721 gttggcaagc acggtctggt ccaccgggtc ccagttcacg acctgggtct tcttgtaggc
      781 catgcctttt tccagcatgc gcaggaacag ccactggttc cagcgatagt attcgggcct
      841 gcaggtggcg agctcgcgcg cccagtcgat ggcgaagccg agcgacttca gctgcttctt
      901 catgtaggcg atgttgtcgt aggtccactg cgccggcggc acgccgttcg ccatcgccgc
      961 gttctcggcg ggcagcccga acgcgtccca gcccatcggc tgcatcacgt tgcagccttt
     1021 catccggtgg tagcgggtga ggacgtcgcc gatggtgtag ttgcgcacgt gccccatgtg
     1081 gagcttgccc gagggatagg gaaacattga gaggcagtag tacttgggct tgccgggcgt
     1141 ttcctgcgcg cggaacgtgc cggtgttctc ccagtgctgc tgggcgtcgc gctcgacgac
     1201 atgaggattg tatttctgct gcatgaaagt gcgcgaataa aaccgcgatt atagccgccg
     1261 cccgacccgc gcgtgcacta taatcagcgc tgcaatttcc ggcgcccgat gtctcccgat
     1321 tttctttccg gtctcaacga acggcagctc gaagcggtca cgcttcccca ccagtcggcc
     1381 ctgattctcg ccggcgccgg cagcggcaag acgcgcgtgc tcaccacgcg cgttgcctgg
     1441 ctgatccaga ccgggcaggc gagcccgcac gggctcgtcg cggttacttt caccaacaag
     1501 gcggcgaagg agatgctctc gcgcatttcc gcgatgctgc cgatcaatac ccgcggcatg
     1561 tgggtcggca ctttccacgg actgtgcaat cggctgctgc gcgcgcattt tcgcgaggcc
     1621 ggcctgccgc aactcttcca gatcctcgac tcgcaggacc agctctctct catcaagcgg
     1681 ctgctgaaag gcctgaacat cgacgaggaa cgtttccccc cgcgccaggt gcaatggttc
     1741 gtgaattcgg ccaaggacga ggggctgcgc gccgcgcagg tcgaggcggg cgacgagttc
     1801 gcgcggcgca tgagggacat ctacgccgcg tatgacgagc agtgcaaccg ggaaggcgtg
     1861 gcggatttcg ccgagctgct gctgcgctcg ttcgagttgc tttccacgca cgcgtcgctg
     1921 agggatcatt atcgtgcccg cttccggcac gtgctggtgg acgaattcca ggacaccaat
     1981 cgcctgcagt acgcctggtt gaagctgctc gcggggggca aggacgccgc ccagggcgtg
     2041 gacaacgcca tcttcgccgt cggggacgat gaccagtcca tctaccggtt ccggggcgcg
     2101 tccgcggcca acatgcagga attgcagcgc gattttgcga tcgagcgcgt gatcaagctc
     2161 gagcagaact atcgctctca gggccatatc ctggatgcag ccaacgcgct gatcggccac
     2221 aaccgccggc ggctcggcaa gaacctctgg accgcggagt cgaaggggga gccactgcgg
     2281 gtattcgacg cggcgaccga tttcgaggaa gccgcgttcg tcgtggacga ggcgcgggcg
     2341 ctgcgcgcag aaggcgtggc gctcgcgcag atcgccgtgc tctaccgttc caacgcgcag
     2401 tcgcgggtgc tggagcatgc actgtttacc gccggcattc cgtaccgggt gtacggcggg
     2461 ctgcgcttct ttgagcgcca ggaagtgaag cacgcgctcg cctacctgcg gctcgtcgcc
     2521 ggctccgccg acgacggtgc tcttgcgcgc gtcatcaact ttcccacgcg cggcatcggc
     2581 gcgcgctcgc tcgaacagct cgccgaggcc gcgaaagagc gcggcgccag tctttggcaa
     2641 gctgcgcttg ccaagtcaca agtcacaagt cacaagtcac aaggagatcg cagcgaagct
     2701 ggcaactcat cactcatcac tcatcactca tcactgccga aaggcatacc cgccttcgtc
     2761 gcgctgatcg ggcagatgcg ggaggcgact gccggactac ccttgcccga gatcatcggg
     2821 ctcgtgatcg aggcgagcgg gctgaacgcc cattaccggt ccgagcggga gggcgccgat
     2881 cgcctggaga acctcgcgga gctgatcaat gccgcggcgg ctttcgtcgc ggaacgcgac
     2941 gtgcagccgg caggcgaggc cgcgggcgtg gacgagcccg atgaattgac cgcgtttctc
     3001 gcccacgccg cgctggaggc gggcgagcac caggcgctgg ccggcgcaga cgcgctgcag
     3061 ctcatgaccg tgcactcggc gaagggtctc gagttccata gcgtgttcgt gagcgggctg
     3121 gaggaggggc tgtttccgca cgagaacagc ctgaccgagg ccgacggcat cgaggaggag
     3181 cggcgcctga tgtacgtggc gctgacgcgc gcgcgccgcc ggctttatct ctccttcgcg
     3241 caaagccgga tgctgcatgg ccagacgcgc tacggcatcg cctcgcgttt tttccgcgag
     3301 attcccgaga acctgttgcg ccacatccgg ccggtcgggg cccgggattc gtctcccggc
     3361 aggagcaccg caaggagcgc cagccacccg ccacccatcg cccatcaccc atcactcgcg
     3421 cccgattcgc cctggcgcat cgggcagagc gtggtgcacg ccaccttcgg cgccggcgtc
     3481 atcgtcaatg cggaagggcg cggcccggac gcgcgcgtgc aggtcaattt caggaacggg
     3541 ggcctgaagt ggctgatgct ggagtacgcg aagctgattc cggcctagcc accacggaca
     3601 tcagccgcag ataatcacgg tgcgttggcc gttacctggt tttgctatcg cagcagagga
     3661 ttaccgccgg tggttgaggc gccgcgtggg gcggtgaaaa cgtccttgta gcttgcgtag
     3721 atcgagcaga tcagaaccgg gatcgaagcg atcagcagcg cggccaccag taccgagccg
     3781 acgacgcctc ctgcggcggg cagcgacatg acaagccaca tcaccaggat cgccagtccg
     3841 tacaccagga acggcgccgt gttcctcagg cacgcggaga acgaaagctt catcgccgcg
     3901 agcggcgcca tctccccgaa tatcaccagc agcggcgcga accagatcag catgatgagc
     3961 ggcaggtagg cggcggcgcc gatggcgagc gcgatcgcga ggctcctcaa cgccgcccgc
     4021 agcgtttcga tgtcggcccc ggcccccttg gacagcacgg tgggcagcat cgaaccgccg
     4081 gccgcgatca gcacgatgcc catcacgatg acggtgccca ccaggtagac gccgccgatc
     4141 gtcaccagcg gcgcggcctg ctgcttgaag cccgcgaaca gatgctggat ttccagcggc
     4201 tcgccgttct cgaccgcgcg gcacccggtc atcagcccgg cgaagaatac cggcgtcaac
     4261 agggtaaaca gcagcgggcc cagtagcggg acgacgagcg ataccaccca gatcagcgcg
     4321 agcgcgacgg tgagcgctat ccacatcgcc gggctcttcc ggaacagggc gaacccgtcc
     4381 acgatccact gccagccctt gcctgccgcg actaccctca cttccatgta cgcctgccct
     4441 cccttggcac ccgctttgag tttccggccg ttgatgggcg ccgcgttaag cggaatcgcc
     4501 tcgaacgcga gaacaattat acgggtcagg gcgccagctg cggcagccgg tcgccggctg
     4561 caatgtgggc tgccagaatg ccgcggaagt gcccgggatc cttggcatgg gtcagttcgc
     4621 ccgggcgcgg caggtaaaaa tcgtagaggc gcgagaccca gaaccgcagc gcccccgcgc
     4681 gcagcatcac cggccacgcg ccgcgttcgg ccgcggtgaa tgggcgcgcg cgggcgtaag
     4741 cctcgagcag cgccgccgcg cgcgtcgcgt cgagcgcgcc gctcccgtcc acgcaccagt
     4801 cgttggcagc gatcgcgacg tcgtagagca gcgcgccagt gcaggcaaaa tagaaatcga
     4861 tgacgccgga aatgcgcccg ttctcgaaca gcacgttatc gcggaagaag tcggcatgta
     4921 tcgcaccgcg cggcagatcg tgcatgcggt catgcaactg aaaccgcact tcctcttcga
     4981 gcagcttcgc gtccgcagcg ccgaggaagg ggacgactgc cggcatgacc gactcccacc
     5041 acctcgggcc acgcgggttg tccatccggc ccgaatacga ggcgccggcc acgtggatgg
     5101 ccgcgagcat cgcgccggcc tgggcgcact gcccggcgtc gggcgcggcg acgtccttgc
     5161 cggtcaggaa gctcgccagt gcagccggct tgccgttcaa ttcgccgagg aagccgccgg
     5221 cgtggttggc caccggcctg gggcagggca cacccttgcc cgccagatgg tcctgcagct
     5281 cgaggtaaaa cggcagttcg tgcgccttca gcttctcgaa cagggtgagg acgtagcggc
     5341 cctgcgaggt cgtgacgaag tagttggtgt tctcgatgcc ggcggcaacg ccctcgaagg
     5401 cggagagggt gccgatggag taattcctga gccagttgcc gagctgttcg cgggtgacgg
     5461 tggtaaaaac agacattgta agcagtaagc ggtaagcaat gagcggctgt cttgccgaca
     5521 ccgcattcta ccgcttaccg ctgaccactt accgctgact tcagaactga aagatcaccc
     5581 acatcggcgg ccgtatgccg ctgtcgtagt tgtcatggcg cgtgaacgtg ccgtcgcccc
     5641 tgtgatcgat catgtagtag ggtttgccgt gcgggggcgt gaccttgacc atgtagagcc
     5701 tgccgccgat gcggtattcc tcgacggtgt cggtgccgcg cttgagaatg gtcacctgcg
     5761 gctcgatcgc ggagtcgaac tcgtagcccg gcggcggagg cggcggctcc ggcaccggct
     5821 gcagcacggg cggcttgccc tgctgcgccg ccaccggcaa ggcggccgcg aggagcaggg
     5881 cggcgagaat tagaagtaga tgcgggaaac gcatgttcat atcgtctttg acacttcgtc
     5941 attatcggag tttcctgccg cgattgatag cggcaatatt tcacgcgccc ccaccaagct
     6001 gacataatgc aagaacccat aacaccatga aacacacgct gcgctatcac ggttttggca
     6061 acacaggcaa tcgtgtcaag tatcgacctc atgctcggcg ttcgtccggg gaaaaatcgt
     6121 gagccaaaac cgcgctagcc cgcaaggggt tgcggccaga atcgcggctc gcattgtcgt
     6181 tcggcttgcc aatatcggca tattggctgc gcctctcttc gcgccatcga cgatactggc
     6241 gcgcaacgca cgcgcgtttc atggtgtcat gggttctaca tgaaaacact actgctcgtc
     6301 gacggttcgt cctacctcta tcgcgctttc cacgcgctcg ctcgcgccaa cttgcgcaac
     6361 aggaaggggg agccgaccgg cgcggtctac ggcgtgatca acatgctgcg tcggctgctg
     6421 aaggacatac cggtcgatta tgccgcctgc gtgttcgacg cgaagggcag gactttccgc
     6481 gacgacctct atgccgatta caaggcgaac cgcccggcga tgccggacga tctcgtggcg
     6541 cagctcgggc ctctcaagga aaccattgcc gcgatgggct ggccgctgct cgagatcgag
     6601 ggagtggagg cggacgacgt aatcggcacg ttagccacgc aggccgggca ccagggcctg
     6661 cgcgtcatca tcgccagcgg cgacaaggac ctcgcgcagc tcgtgaatcc cgtcatcacg
     6721 atggagaatg ccgaccccag gaccggcggg cgcgagattc tcgacgaggc cggcgtggcg
     6781 aaaaaattcg gcgtcccgcc cgggcgcatc atcgactacc tgaccttgat cggcgacagc
     6841 gtggacaacg tgccgggcgt ggccgacgtc ggcccggtga ccgccgtgaa atggctcgcc
     6901 gaatacggca
//
LOCUS       DBNGSG010000191         4323 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4477969, whole genome shotgun sequence.
ACCESSION   DBNGSG010000191 DBNGSG010000000
VERSION     DBNGSG010000191.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4323)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4477969"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..366
                     /locus_tag="AC158Q_05055"
     CDS             175..366
                     /locus_tag="AC158Q_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339175.1"
                     /translation="MFAASADGFLQGEGEHKHGVQARILYPDAFPEDLRPIVGDFHTR
                     CHHPSPSEAQFDGRLKTGA"
     gene            363..1379
                     /locus_tag="AC158Q_05060"
     CDS             363..1379
                     /locus_tag="AC158Q_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HIR3339176.1"
                     /translation="MTTARVSLAVLAAGALLAGLIAVAFSVGRFPVSTGDVASVLWAS
                     LTGREHGLDATLSTVVLKIRGPRVAAALLIGAALAAAGAAYQNMFRNPLVSPDILGVS
                     AGAAVGAVLGIFLSLNVIMIQSLAFAFGLGAVALVYVTANAVRGHDPLLVLVLAGVVV
                     GSLLGACVALMKYLADPYNQLPAITFWLLGSLASTNPDDVWSMLPLMLVGLIPMWLLR
                     WRINLLSLDDEEARALGVETGRIRLVVIVAATLMTSAAVAISGVIGWIGLVIPHFARL
                     FVGPDFSRLLPTAILLGAAFLLGVDTLARTVARIEIPLGVLTALIGTPLFLWQLAAAR
                     RGWQ"
     gene            1575..2360
                     /locus_tag="AC158Q_05065"
     CDS             1575..2360
                     /locus_tag="AC158Q_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012836979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3339177.1"
                     /translation="MRLEAKHLDFGYGDRRVGSDVSLALEAGEVLCLLGPNCSGKTTL
                     FKTLLGLLPPQGGRVLLDGADMKQQRRGEVARLVSYVPQAHATFFPFTVREVVLMGRT
                     SHLGVFSSPSRRDLGIAQAAIDRMGLGGLADTVYTRISGGERQLALIARALAQDARII
                     VMDEPTANLDFGNQVRVIEHIRSLAGTGMGVLLSTHDPDQAFECADRVALLHEGRLAR
                     VGTPADTITAVSLKQIYGVGVVVTHVMLPDGATRPVCIPSVAD"
     gene            2395..2763
                     /locus_tag="AC158Q_05070"
     CDS             2395..2763
                     /locus_tag="AC158Q_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3339178.1"
                     /translation="MPRYPGLLLRVLGAQNPAMGPGKARLVALIAGTGSISAAAREMG
                     MSYRRAWQLVEALNRSFNEPVVVTATGGKRGGGAVVTDFGKRLVARYHDMEDKASAAI
                     ASDLRAFSAHLRKPSKRKSR"
     gene            complement(2769..3521)
                     /locus_tag="AC158Q_05075"
     CDS             complement(2769..3521)
                     /locus_tag="AC158Q_05075"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR3339179.1"
                     /translation="MQTQEILTLCAAFFVVATLYTTVGHAGASGYLAVMALVGLAPEV
                     MRPTALVLNIIVAALAVYRFRRARFFSWAGLWPFLIGSVPFAAAGGIQSLSRGTYYVA
                     MGAVLLIASAYLVWRAFGSRPVMEEHVVRVRKVPAVLMGAALGFLSGLIGVGGGIFLS
                     PVLLLLGWAGPRTTAGISAPFILVNSAVALAAGSLTVQTLPGELPLLAAAALAGAFLG
                     TWLGLERLKQKGLLVTLAGVMSLAGAKLLLTA"
     gene            3591..4085
                     /gene="mobB"
                     /locus_tag="AC158Q_05080"
     CDS             3591..4085
                     /gene="mobB"
                     /locus_tag="AC158Q_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HIR3339180.1"
                     /translation="MKIFGFAGWSGSGKTTLIEKLIPLFVARGIKVSLIKHAHHTFDV
                     DQPGKDSWRHRHAGCSEVLVTSSKRWVLMHELRGAPEPGFAELIERVSPCDLVLIEGF
                     KREHLPKLEVYRAAVGESLLHPRDPSIVAIASDQKIDSRLPQFNLDDTPAIADFVLRR
                     CGLA"
     gene            4112..>4323
                     /locus_tag="AC158Q_05085"
     CDS             4112..>4323
                     /locus_tag="AC158Q_05085"
                     /inference="COORDINATES: protein motif:HMM:NF014636.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIR3339181.1"
                     /translation="MITILYFARLREALGMSSEQIALPAGVRDLEGLRALLVARGGAW
                     EQELAPNKPVRAAVNQSMAVGDVPVA"
BASE COUNT          733 a         1345 c         1448 g          797 t
ORIGIN      
        1 ggctttcatc gcctggatcg tgtcgcccga cgggcagaaa gccatcactg attacaggat
       61 tggcggcgaa cagttgttct tcgcgaactc caacgaccgg gacgtgtaac ggttgttcgg
      121 ggccagccgg gaggactttg ccgtggcatg tccctgagcc cgaaatgacg cagattgttt
      181 gccgcgagcg cggacggttt tttgcaggga gagggtgagc acaaacacgg cgttcaggcg
      241 cgcatcctgt atcctgatgc cttcccggag gacctgaggc cgatcgtggg cgatttccac
      301 acgcgttgcc atcacccgtc gccgagcgaa gcgcaattcg acggtcggct caagaccggc
      361 gcatgacgac cgctcgcgta tccctggcgg tgcttgccgc cggcgctttg ctggccggcc
      421 tcatcgccgt cgctttctcg gtgggccggt ttccggtttc gactggtgac gtggcgagcg
      481 tgctgtgggc cagtcttacc gggcgcgagc acgggctcga tgcgaccctc tcaaccgtgg
      541 tactgaagat ccgcggaccg cgcgtcgccg cggcactatt aatcggagcg gcgctcgccg
      601 ccgcgggtgc cgcctaccag aacatgttcc gcaatcccct ggtgtctcct gacattctcg
      661 gcgtatccgc aggagcggcg gtcggtgcgg tgctgggaat ctttctctcc ctcaacgtaa
      721 tcatgatcca gtcgcttgct ttcgcgtttg ggctgggcgc ggtggcgctg gtctacgtaa
      781 cagccaacgc ggtgcgcggg cacgacccgc tgctggtgct ggttctggca ggcgtggtgg
      841 tgggctcgct gctcggggcg tgcgtcgcct tgatgaaata cctggccgat ccctacaacc
      901 agctgccggc gatcactttc tggctgcttg gcagcctcgc ctcgacgaac ccggacgacg
      961 tctggtccat gttgccgctc atgctggtcg gcttgattcc gatgtggctg ctgcgctggc
     1021 gcatcaacct gctgtcgctg gatgacgagg aggcgcgggc gctgggcgtc gaaaccgggc
     1081 gcatacgcct ggtcgtgatc gtggcggcca cgctcatgac ttccgccgcc gtggccattt
     1141 cgggcgtgat tggatggatc ggactcgtca tcccgcattt cgcacgcctg ttcgtgggcc
     1201 cggatttcag ccgactgttg ccgacggcca tcctgctcgg cgccgctttt ctgctgggtg
     1261 tggacacgct ggcgcgcacg gtggcgcgga tcgagatccc gctcggcgtg ctgacggccc
     1321 ttatcggcac gccgctgttc ctgtggcagc ttgcagcggc gcggcgcggc tggcagtaaa
     1381 ccagaaaatg aaccgccaag accgccaaga agaacagaat caatattcac aagattcatg
     1441 cgggaatatg aaccccacta atggatttga gagtgaaatt ctcttggaaa gaccgttgat
     1501 ttcttgcgat tgtgcacatt gattgattag ggtgttttct tggcgggctt ggcggttgga
     1561 ttgagggtgt cgacttgaga ctagaggcga agcatctcga ttttggctac ggcgaccgcc
     1621 gcgtcggcag cgatgtatcg ttggcgctgg aagccgggga ggtgctgtgc cttctcggac
     1681 ccaactgcag cggcaagact acgctgttca agacgctgct cgggctgttg ccaccgcagg
     1741 gcgggcgggt cctgctggac ggcgcggaca tgaagcaaca gcggcggggc gaggtggcgc
     1801 ggctggtaag ctacgtgccg caggcgcacg cgacgttttt cccgttcacc gtgcgcgaag
     1861 tggtgctgat ggggcgcacg tcgcacctgg gcgtgttttc ttcgccctcg cgccgtgacc
     1921 tgggcattgc ccaggctgcg atcgatcgca tggggctggg cgggcttgcc gacactgtct
     1981 atacgcgcat ctccggcggc gagcgccagc tcgccctgat tgcccgggcg ctggcgcagg
     2041 acgcgcgcat catcgtcatg gacgagccca ccgccaacct cgattttggc aaccaggtgc
     2101 gcgtcatcga gcatatccgg tcgctggcag gcacgggcat gggggtgctc ctgtccaccc
     2161 acgatcccga tcaggcattc gaatgcgccg accgggtggc cctgttgcac gaggggcggc
     2221 tggcccgggt cggaacgccc gccgacacca tcaccgcggt gagcctgaag cagatctacg
     2281 gcgtcggggt agtggtcacg cacgtcatgc tgccggatgg cgcgacgcgg ccggtctgca
     2341 tcccatcggt tgctgattag agcctattat tgagataggc tctagaatca cgggatgccg
     2401 cgctaccctg gattgctcct tcgcgtgctg ggcgcgcaga acccggcgat ggggccgggc
     2461 aaagcccggc tggtcgcgct gatagccggc acgggctcga tatccgccgc cgcgcgcgag
     2521 atgggcatgt cgtaccggcg cgcctggcag cttgtcgaag cgctgaaccg gagcttcaac
     2581 gagcccgtgg tggtgacggc caccggtggc aagcgtggtg gcggcgcggt cgttacggat
     2641 ttcggcaagc gcttggtcgc gcgctaccac gacatggaag acaaggcctc ggctgccatc
     2701 gcatcggacc tcagggcgtt ttcggcccat ttgcgcaagc cctcaaaacg caagtcccgc
     2761 tgaccgcctc acgccgtaag cagcagtttc gcgccggcca gcgacatcac cccggcgagc
     2821 gtgaccagca gtcccttctg cttcaatcgc tcgagcccga gccaggtgcc gaggaaagca
     2881 ccggccaggg ctgcggcggc gaggagcggc agctcgccgg gcagtgtctg cacggtaagc
     2941 gagcccgccg ccagcgccac cgctgaattc accaggatga atggggcgga aatgccggcg
     3001 gttgtcctgg gtccggccca gccgagcagg agcagaacgg ggctcaggaa aatgcctcct
     3061 ccgacaccga tcaggcccga caggaagccc agcgcggccc ccatcaatac cgccgggact
     3121 ttcctcacgc gcaccacgtg ctcttccatg acgggacgcg atccgaaagc gcgccagacg
     3181 agataggcgg acgcaatcag cagcaccgca cccatcgcca cgtagtaggt gccgcgcgac
     3241 aggctctgga ttcctcccgc ggcggcgaac ggcaccgagc cgatcaagaa aggccacaag
     3301 ccggcccagg agaagaaacg cgctcgccga aatcgataga ccgccagggc agccacgatg
     3361 atattcagca ccagcgctgt cggccgcatg acctccggag ccaggccgac caaagccatc
     3421 acggcgaggt aaccggatgc gcccgcgtgc ccgacggtgg tatagagcgt cgccaccacg
     3481 aagaacgcag cgcaaagggt gagaatctcc tgcgtttgca ttttctgacc atagcatgtt
     3541 acgaccgtta tatctagacg ggaataacgc gcctcggcta tgatagtgcc atgaaaatct
     3601 tcggtttcgc cggctggtcc gggagcggca agacgacgct gatcgagaag ctgattccgc
     3661 tgttcgtcgc gcgggggatc aaggtgtccc tcatcaaaca cgctcaccac acgttcgacg
     3721 tggaccagcc ggggaaggac tcctggcgtc accgccacgc cggctgcagc gaggtgctgg
     3781 tcacttcgtc gaagcgctgg gtgctgatgc acgagctgcg gggcgctccg gagccaggct
     3841 ttgcagagct catcgagcgc gtttcgccct gcgatctggt gctgatcgag gggttcaagc
     3901 gtgagcacct gcccaagctc gaggtttacc gcgccgcggt gggggagtcg ctgctgcacc
     3961 ctcgggatcc cagcatcgtg gcgattgcaa gcgatcagaa gatcgacagc cggctgcccc
     4021 agttcaacct cgatgatacg ccggcaatcg cggatttcgt gctgcgccgc tgcggcttgg
     4081 cgtagcggtt ttcagacggc atactgctcc gatgatcacg atcctctact ttgcacggct
     4141 gcgcgaggcg ctcggcatga gttcggagca gatcgcgctg ccggccggcg tgcgcgacct
     4201 ggaaggcctg cgcgcgttgc tcgtcgcgcg cggtggtgcg tgggaacagg agctggcgcc
     4261 caacaaaccg gtgcgcgcgg cggtgaacca gtcgatggcg gtcggcgatg tgccggttgc
     4321 cga
//
LOCUS       DBNGSG010000192         4114 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4478383, whole genome shotgun sequence.
ACCESSION   DBNGSG010000192 DBNGSG010000000
VERSION     DBNGSG010000192.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4478383"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..1082)
                     /gene="mltB"
                     /locus_tag="AC158Q_05090"
     CDS             complement(57..1082)
                     /gene="mltB"
                     /locus_tag="AC158Q_05090"
                     /inference="COORDINATES: protein motif:HMM:NF024798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HIR3339182.1"
                     /translation="MDVAKGQGNPHMWPALVFAALMLGIGTLVPSAALAQKPLPPEIE
                     AFVDEMAQKHQFDAETLRRLFGQVQPRPAILRAISAPGTARPWHEFRRLNVDAAHING
                     GTAFWRQHAAVLARASREYGVPEEIIVATIGIETLYGRNTGNHRVLDALTTLAFQYPP
                     RAELFRAQLEEYLLLARESGFDMMRIRGSYAGAIGIPQFLPGSYRRYAVDFDGDGKRD
                     LMGTVADAIGSVANYYKAHGWRAGEQVVVPAHSGGSDAEALVGMGIKPQLKFAELKRS
                     GVVPAGPVADEEAAALFVVETETGPRYWLGLNNFYVITRYNRSVNYALAVYELARELR
                     ALAMPED"
     gene            complement(1127..1216)
                     /locus_tag="AC158Q_05095"
     tRNA            complement(1127..1216)
                     /locus_tag="AC158Q_05095"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1180..1182),aa:Ser,seq:cga)
     gene            complement(1393..2667)
                     /gene="serS"
                     /locus_tag="AC158Q_05100"
     CDS             complement(1393..2667)
                     /gene="serS"
                     /locus_tag="AC158Q_05100"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HIR3339183.1"
                     /translation="MLDVQLLRSDLSGVTRRLADRGYALDTAAFEALEAGRKKVQVET
                     QELQARRNQISKQVGQMKARGEDASRLMAQVNAEADRLKALEQELENIQARVNDFLLG
                     VPNVPHASVPAGKSAEDNPETRRNGEPRKFAFPVKDHVDIGQGLRLLDFDAATRIAGA
                     RFTLLKGGLSQMHRALAQFMLDVHTREHGYTEVYAPYLVNADSMIGTGQFPKFQEEQF
                     AVAADNLYLIPTAEVPVTNIVRGQILGEADLPLKFVCHTPCFRREAGSYGKDTRGMIR
                     QHQFDKVELVQIVHPAKSYELLEELTSHAETILKRLDLPYRVVTLCTGDMGFSAAKTY
                     DIEVWLPGQKAYREISSCSNFESFQARRMQARFRNEKHKTEFVHTLNGSGLAVGRTLV
                     AVLENYQNADGSVSIPEALRPYRGGTTAIKPN"
     gene            complement(2754..4070)
                     /locus_tag="AC158Q_05105"
     CDS             complement(2754..4070)
                     /locus_tag="AC158Q_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014751518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HIR3339184.1"
                     /translation="MNDLFKKRAPDAPLADRLRPRTLDELVGQAHLLGQGKPLRLAFG
                     AGKPHSMILWGPPGVGKTTLARLMAQAFDAEFIAISAVLSGVKEIRDAVTRAELALQQ
                     SGRSTILFVDEVHRFNKAQQDAFLPHVERGLLTFIGATTENPSFEVNSALLSRAAVYV
                     LQPLTEEELGQLFDRARSAVLSGLVFAPEARALLIASADGDARRLYNMLEQAGIAAEE
                     ARRNEVDEAFVRDALAQRLRRFDKGGEQFYDQISALHKSVRGSDPDAALYWLTRMLDG
                     GADPLYVGRRLVRMASEDIGLADPRALRVALDACETYERLGSPEGELALAEAALYMAI
                     APKSNAAYKAFGAARELVRNDSTRPVPPHLRNAPTRLMKDLGYGRDYRYAHDEPDAYA
                     AGENYFPEGMKKPEFYHPTGQGLEARIREHLEHLRQLDAGAKRKKQ"
BASE COUNT          689 a         1382 c         1270 g          773 t
ORIGIN      
        1 gaaatagacc acctcgtcgc cttccgcaac gaagcggccg acgccggccc cgccgcctag
       61 tcctcgggca ttgccagggc gcgcagctcg cgcgccagct cgtagaccgc cagcgcgtaa
      121 ttgacgctgc ggttgtagcg cgtgatcacg tagaagttat tgaggccgag ccagtaacgc
      181 ggccccgttt ccgtttccac cacgaacagc gccgcggcct cctcgtccgc aaccggaccg
      241 gccggcacca cgccggagcg cttcagctcc gcaaatttga gttgcggctt gatgcccatc
      301 cccaccaagg cttccgcatc gcttccgccg gaatgggccg gcacgacgac ttgctcaccg
      361 gcgcgccagc cgtgcgcctt gtagtagttc gccacgctgc cgatcgcgtc agcgacggtg
      421 cccatgagat cgcgcttgcc gtcgccgtcg aagtccacgg cgtagcggcg atagctgccc
      481 ggcaggaact gcggtatgcc gatggccccc gcataagagc ccctgattct catcatgtcg
      541 aatcctgact cccgcgcgag caggagatac tcctcgagct gcgcccggaa cagctcggcg
      601 cgcggcgggt actggaatgc aagcgtggtc agagcgtcca ggacccggtg gtttccggtg
      661 ttccgcccat agagcgtctc gatgccaatg gtggcgacga tgatctcctc gggcacgccg
      721 tactcccggc tcgctcttgc caggacggcc gcatgctggc gccagaatgc ggtgccgcca
      781 ttgatgtgcg cggcatcgac attgagcctg cggaactcgt gccatggccg cgccgtaccg
      841 ggagcggata tggcgcgcag gatagctggc cgcggctgca cctggccgaa cagccggcgc
      901 aatgtttcgg catcgaactg gtgtttctgc gccatttcgt cgacaaaggc ctcgatctcc
      961 ggcggcaaag gcttctgggc aagtgccgca gaagggacga gagtcccgat gccgagcatc
     1021 agtgcggcaa aaaccaacgc cggccacatg tggggatttc cctgtccttt ggcaacgtcc
     1081 atgctctcag cgccaacaaa aaagcgacct cggggtcgct gtttgttggc ggagagggtg
     1141 ggattcgaac ccacgatacg cttgcgcgta ttccggtttt cgagaccggc gcgttcaacc
     1201 actctgccac ctctccggta cttcagacga catcaatcct gcacggaaaa ccggaaccgg
     1261 ctttggctcc ggccggccct gaagggtctt aacttgcccc ttggtaccga ccaaggggca
     1321 gttagcctcg gccccaacga gccgacgctg ttttcgcgaa ggcgcgattc taccaacgcg
     1381 cgccgcgccc gcctagttcg gcttgattgc agtcgtgccg cccctgtacg gccgcagcgc
     1441 ctccggtatc gagacgctgc cgtccgcatt ctggtagttc tcaagcacgg ccacgagcgt
     1501 tctcccgacc gccagtccgg agccgttcag cgtatgcacg aactcggtct tgtgcttctc
     1561 gttgcggaaa cgcgcctgca tgcggcgcgc ctggaacgac tcgaagttgc tgcacgagga
     1621 aatttcccgg tacgccttct gccccggcag ccagacctcg atgtcgtagg tcttggccgc
     1681 ggagaaaccc atgtcgcccg tgcacagggt cacgacgcgg tatgggagat ccagtctttt
     1741 cagaattgtt tccgcgtggg aagtcagttc ttccagcagt tcgtaggact tcgcgggatg
     1801 cacgatctgc accagctcga ccttgtcgaa ctggtgctgg cggatcatgc cgcgcgtgtc
     1861 cttgccgtac gagccggcct cgcgccggaa gcacggcgta tggcaaacga acttgagcgg
     1921 caggtccgcc tcaccaagga tctgcccccg aacgatgttg gtgaccggca cctcggcggt
     1981 agggatcagg tacaggttat ccgccgccac cgcgaactgc tcctcctgga acttcggaaa
     2041 ctgacccgtg ccgatcatgc tgtcggcgtt gaccaggtac ggcgcataga cttccgtgta
     2101 gccgtgctcc cgcgtgtgga cgtcgagcat gaactgcgcc agcgcgcgat gcatctgcga
     2161 cagcccgccc ttcagcagcg taaagcgcgc gccggcgata cgcgtcgcgg cgtcgaaatc
     2221 gagcagcctg agcccctgcc cgatgtcgac gtggtccttc acgggaaaag cgaatttgcg
     2281 cggctcgccg ttcctgcggg tctcggggtt gtcttccgcg gattttcccg ccggcacgct
     2341 tgcgtgcgga acattgggaa cgccgagcag gaaatcgttc accctggcct ggatgttctc
     2401 cagttcctgc tccagcgcct tcagacggtc cgcctcggca ttgacctgcg ccatgagccg
     2461 ggaggcatcc tctcccctgg ccttcatctg gccgacttgc ttggagatct ggtttcgcct
     2521 tgcctgcagc tcctgcgttt cgacctgtac tttcttgcgg cccgcttcca gcgcctcgaa
     2581 tgcggcagtg tcgagcgcgt agccgcgatc ggcgagccgc cgggtcacgc ctgacaagtc
     2641 gctgcgcagg agttgaacat caagcatgcg ggtgttgttg cattatcaat tcgcgggaca
     2701 ttatctcgga gttcgggagg cgccgccaga gcttgacacg cctgggctgc cgattactgc
     2761 ttcttgcgct tcgcgccggc atcgagctgg cgcaagtgct cgagatgctc gcgtatcctg
     2821 gcttccagcc cctgcccggt cgggtggtag aactccggct tcttcatccc ttcgggaaaa
     2881 tagttttcac ccgccgcgta agcatccggc tcgtcgtggg cgtagcggta atcgcgccca
     2941 tatccgaggt ccttcatcag acgggtcggc gcgttgcgga ggtgcggcgg caccggacgg
     3001 gtcgaatcgt ttcgcaccag ttcccgtgcc gctccaaacg ccttgtaagc cgcgttgctc
     3061 ttcggggcga tcgccatgta cagcgccgct tccgcgagcg cgagttcccc ttccggcgac
     3121 cccagccgct cataggtttc acaggcatcc agcgcgacgc gcagcgcgcg cgggtcggca
     3181 agcccgatgt cctcggacgc catgcgcaca agccgcctgc ccacatagag cggatcggcg
     3241 ccgccatcca gcatgcgagt gagccagtac agcgcggcat ccgggtcgga cccgcgcacg
     3301 gacttgtgca gggccgagat ctggtcgtag aactgctccc ctcccttgtc gaagcggcgc
     3361 aatcgttgtg ccagggcatc tcgaacgaac gcttcatcca cttcgtttcg gcgcgcctct
     3421 tccgccgcaa tcccggcctg ctccagcatg ttgtagagcc ggcgcgcgtc gccgtcggcc
     3481 gaggcgatca gcaatgccct cgcttcgggc gcaaaaacaa gtccgctcag caccgcgctt
     3541 cgagcccggt cgaacaactg ccccagttct tcctccgtga gcggctgcag gacgtagact
     3601 gccgcccgcg acagcagcgc gctgttcacc tcgaacgaag ggttctcggt agtggcgccg
     3661 atgaaagtca gcaatccccg ctccacgtgc ggcaggaacg cgtcctgctg cgccttgttg
     3721 aagcggtgca cctcatcgac gaacaggatg gtgctgcggc cggattgctg caaggcgagt
     3781 tctgcccggg taacggcatc gcggatttcc ttcacgcccg acagcaccgc ggagatggcg
     3841 atgaactcgg catcgaaagc ctgcgccatc aggcgcgcaa gcgtggtttt gcccacgccc
     3901 ggcgggcccc acaaaatcat ggaatggggc ttgccagccc cgaacgcaag ccgcagcggc
     3961 ttgccctgcc cgagcaggtg cgcctggcct accagttcat cgagcgtgcg cgggcgcagc
     4021 cgatcggcga gcggggcatc cggcgcgcgc ttcttgaaca ggtcattcac tgatgacgtc
     4081 cgctcccgcg ggcggcgtga agcgaaacgc ttcc
//
LOCUS       DBNGSG010000193         3258 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1762220, whole genome shotgun sequence.
ACCESSION   DBNGSG010000193 DBNGSG010000000
VERSION     DBNGSG010000193.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1762220"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="AC158Q_05110"
     CDS             complement(<1..739)
                     /locus_tag="AC158Q_05110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR3339185.1"
                     /translation="MPKSATKNPARPAVLDRLRQAVGPQGLITDPGEVDPHTVDWRGY
                     YHGATPAVICPASSSEVAAVVGICADTRTAIVPQGGNTGMCGASVPGGDGSAIVINLA
                     RMNRVLQVDALNNTLSVEAGCILANIQQAARDAGRLFPLSLGAEGSCQIGGNLSTNAG
                     GVNVLRYGNTRELALGLEVVLPDGRIWDGMRALRKDNTGYDLKQAFIGAEGTLGIITA
                     AVLKLFPLPRSRATAWACVPSPDAALEL"
     gene            complement(985..>3258)
                     /gene="acnA"
                     /locus_tag="AC158Q_05115"
     CDS             complement(985..>3258)
                     /gene="acnA"
                     /locus_tag="AC158Q_05115"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HIR3339186.1"
                     /translation="TPDALRKNIEIEFQRNRERYQFLKWSAQAFKTFQIIPPGNGICH
                     QINLEYVSQVVWQKDGMFYPDSLVGIDSHTTMVNGLGVLGWGVGGIEAEAAMLGQPMY
                     FLEPDVVGVHLTGKLNDGVTATDLVLAIVKLLRDTKVVGKFVEYFGDGAASLSVPDRA
                     TLGNMSPEYGATCGFFPVDDVSCEFLKMTGRGNIVPAVQAYWKAQGMYGMPKKGDIDY
                     SQVIDFDLSTVKPAVSGPRRPHDHLDLPALKGKFKELMGKAFTDGGYGKGNEMGKRVP
                     TSKTGVDVGHGDVLISAITSCTNTSNPGVLLGAGLLAKKAVEKGLKPHPRVKTSLGPG
                     SLAVTQYLKSSGLLPYLEQLGYYVAGYGCTTCIGNSGPLDGHIDEAVSKNDIVAAAVL
                     SGNRNFEARIHQNIKANFLMSPPLVVAFGLAGTVEIDMTKDPIGTGKDGKPVYLKDIW
                     PSTKEVGEVMKYATDPEMYRKLFSAFGEGNPMWDEIPASVGATYKWDEKSTYVRLPPY
                     FEGFTMKPGKTGDVKGARVLGIFGDAITTDHISPAGGIRPTAPAGKYLQERGVAVADF
                     NTYGARRGNHEVMMRGTFANIRIKNLMVPGTEGGITLHQPSGEKTSIYEAAMRYKKDG
                     VPSVVFGGDEYGQGSSRDWAAKGPQLLGVKACIVRNFERIHRANLVFMGVLPLEFKPG
                     TTVQTLKLDGSEVIDITGIEKSITPMQDVTMTITRKDGTRENVPLTLRIDTPIEVDYY
                     HHGGILPYVLRELIAAA"
BASE COUNT          520 a         1028 c         1087 g          623 t
ORIGIN      
        1 gcagctccag agcggcgtcc ggcgagggca cgcaagccca ggccgtggct ctggagcggg
       61 gaagggggaa gagtttcagc actgccgcgg taatgatgcc gagagtgcct tccgcgccga
      121 tgaaagcctg cttcagatcg tatccggtgt tgtccttgcg cagcgcccgc atgccgtccc
      181 agatgcggcc atccggaagc accacttcca ggcccagcgc gagctcgcgt gtgttgccgt
      241 agcgcagcac gttcaccccg ccggcattgg tcgagaggtt gcccccgatc tgacagctgc
      301 cttccgcgcc cagggaaagc gggaacagcc ggcctgcgtc gcgcgcggcc tgctggatgt
      361 tcgcgagtat gcagccggcc tcgacactga gagtgttatt gagcgcgtcc acttgcagta
      421 cgcggttcat gcgcgcgaga ttgatcacga tcgcgctgcc gtccccgccc ggcacggatg
      481 cgccgcacat gcccgtattg ccgccttgcg gcacgatggc cgtgcgcgtg tcggcgcaga
      541 tccccaccac tgcggcaacc tcgctggaac tggcgggaca aatcactgcg ggcgtggcgc
      601 cgtggtagta acctcgccag tccacggtat ggggatcgac ctcgccggga tcggtaatca
      661 agccttgcgg gccgaccgcc tggcgcaacc ggtcgagtac ggccgggcgc gcagggtttt
      721 tcgtggcgga cttgggcata aaaatgttct ccgggcgcat tgtaccgtga tcgcggcatc
      781 gcgttcgccc attgtcccgt acggcctcgc tggctgatca aggcgcacag gacgcgcggc
      841 gccggtcgat acgaagagcg gggacctgat cagggtgctc gcggctgacc ggtgacctgc
      901 tgaaaaaaac aggccgatgc cttgtgagca tcggcctgcg cgatagccgg cgcccggtaa
      961 ggcgccggcc gtagcctgaa tggattacgc ggcggcgatg agttctcgca agacgtaagg
     1021 caatatgccg ccgtggtggt agtagtccac ctcgatcggc gtgtcgatgc gcagcgtgag
     1081 cggcacgttc tcccgggttc cgtccttgcg cgtgatcgtc atggtgacgt cctgcatcgg
     1141 cgtaatgctc ttctcgatgc cggtgatgtc gatcacttcc gagccgtcga gcttcagcgt
     1201 ctgcacggtc gtgcccggct tgaattcgag cggcagcacg cccatgaaca cgaggttggc
     1261 gcggtgaatg cgctcgaagt tgcgcacgat gcaggccttg acgccgagca gttgcggccc
     1321 tttcgccgcc cagtcgcgcg aagagccctg gccgtattcg tcgccgccga acacgaccga
     1381 gggcacgcca tccttcttgt agcgcatcgc ggcctcatag atcgaggttt tctcgccgga
     1441 gggctggtgc agggtgatcc cgccctccgt gcccggcacc atcaggttct tgatgcggat
     1501 attggcgaac gtgccgcgca tcatgacttc gtggttgccg cggcgcgcgc cgtaggtatt
     1561 gaaatcggca accgcgacgc cgcgttcctg caggtatttg ccggccgggg ccgtcgggcg
     1621 gataccgccc gccgggctga tgtggtcggt ggtaatcgcg tcgccgaaga tgcccaggac
     1681 gcgcgcgcct tttacatcgc cggtcttgcc cggtttcatc gtaaagccct cgaaataggg
     1741 cggcaagcga acgtaagtcg atttttcgtc ccacttgtaa gtggcgccga ccgaagcggg
     1801 gatctcgtcc cacatcgggt tgccttcgcc gaacgccgag aagagcttgc ggtacatctc
     1861 cgggtcggtc gcgtacttca tgacttcacc cacctccttg gtcgagggcc agatgtcctt
     1921 gaggtagacc ggcttgccat ccttgccggt gccgatcggg tccttggtca tgtcgatctc
     1981 gacggtgccg gcgaggccga acgccacgac cagcggcggg ctcatcagga agttggcctt
     2041 gatgttctgg tggatacgcg cctcgaagtt gcggttgccc gacagcacgg ccgcggccac
     2101 gatgtcgttc ttgctcaccg cttcgtcgat gtggccgtcg agcggccccg aattgccgat
     2161 gcaggtggtg cagccgtagc ccgcgacgta gtagccgagc tgctcgaggt agggcagcag
     2221 cccggagctc ttcaggtact gagtcacggc gagcgagccc ggtcccagcg acgtcttcac
     2281 gcggggatgg ggcttgaggc ctttctcgac cgctttcttg gcgagcagcc cggcgcccag
     2341 cagcacgccc gggttggagg tgttggtgca ggaagtgatg gccgagatca gcacgtcgcc
     2401 gtggccgacg tccacgccgg tcttgctcgt cggcacgcgc ttgcccatct cgttgccctt
     2461 gccgtagccg ccgtcggtga aggccttgcc catcagttcc ttgaacttgc ccttcagcgc
     2521 cggcaggtcg agatgatcgt gcgggcggcg cgggccggag acggccggct tgaccgtgga
     2581 gagatcgaaa tcaatgacct ggctgtagtc gatgtcgcct ttcttgggca tgccgtacat
     2641 gccctgggct ttccagtagg cctggaccgc cggcacgata ttgccgcgcc ccgtcatctt
     2701 gaggaactcg caggacacgt cgtcgaccgg gaagaagccg caggtcgcgc cgtactcggg
     2761 cgacatgttg ccgagcgtgg cgcggtcggg aaccgacagc gacgcagccc cgtcgccgaa
     2821 atactcgacg aacttgccga ccactttcgt gtcgcgcagc agcttgacga tcgcgagcac
     2881 gagatcggtg gcggtcacgc cgtcgttgag cttcccggtc aggtgcacgc cgacgacatc
     2941 gggttcgagg aagtacatcg gctggcccag catcgcggct tcggcctcga tgccgccgac
     3001 gccccagccc agcacgccga ggccgttcac catcgtggtg tgggagtcga tgccgaccag
     3061 gctgtcgggg tagaacatgc cgtccttctg ccacactacc tgggagacgt actcgaggtt
     3121 gatctggtgg cagatgccgt ttccgggcgg gatgatctgg aaggtcttaa acgcctgcgc
     3181 gctccacttc aggaactggt agcgctcgcg gttgcgctgg aactcgatct caatgttctt
     3241 gcgcagcgca tccggtgt
//
LOCUS       DBNGSG010000194         4138 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2611108, whole genome shotgun sequence.
ACCESSION   DBNGSG010000194 DBNGSG010000000
VERSION     DBNGSG010000194.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2611108"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1628)
                     /locus_tag="AC158Q_05120"
     CDS             complement(<1..1628)
                     /locus_tag="AC158Q_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="beta strand repeat-containing protein"
                     /protein_id="HIR3339187.1"
                     /translation="MNNGMIKSWTVLIAFATFAVASEPAWAVAKTWRSGGGANATNWN
                     RAQNWAPNGVPAAGDDVTIPTVPDNGTGFPVLNTSPTILSLTIQSGASVAGAAGQSLS
                     VTGTVTGAGSLTGGASPFTVVGDMTVSTLTSTSTVTYNGTGAQVIGGGVHSYTNLTIN
                     KAGGTATFASNAQVAGDLTLTAGTLDLATFTANRTAAGGTITVGTGATLIIGGTNTFP
                     ANYTTATLGATSTVNYNGTAQNVANAVGAPGYGHLTLSGSSTKTALGTFTVRGDLSIS
                     SVTFAGDTFTHTVNGNVSNTGTHTVSTGNITLSGGAAVHSLTGAGTYGNLILNDANGA
                     TLGGSPTLSGTLTLTSGTFAVGANKLTLNGPNTAGTTSNMSTTASSSLEFGGISAGVS
                     IPSGVVQLLDLTINNSNGITLASSPIVNGTLTFISGNLNTTAANTLSIESAGSISGAA
                     PSQHVVGNLARVFPATAGTSFTYAVGDGTNFTPVTITFPAAPTTGSLTVATPGSPAAD
                     HPDTTGETSGIDAAKSINRYWTLKGSTLNGTCTAA"
     gene            complement(1625..2026)
                     /locus_tag="AC158Q_05125"
     CDS             complement(1625..2026)
                     /locus_tag="AC158Q_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339188.1"
                     /translation="MKKKADSGFALVTAIFLLVVLAALGAFMVTLSGVHQTTPTQALD
                     ATRVYYGAKAGLDWGIQQAIAAGSCAGSTPLTLTGNGLSSVTTTVTCTTGGLTHTGGN
                     VYVITSTAERGAFGSINYARRRMQATVTNIP"
     gene            complement(2023..2940)
                     /locus_tag="AC158Q_05130"
     CDS             complement(2023..2940)
                     /locus_tag="AC158Q_05130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HIR3339189.1"
                     /translation="MIPGAFRLRRNTRGVTLVELVVVITITSIVAGMVAVFVLRPVQG
                     YVDLSRRASLVDSAESALRRMARDIRIALPNSVRVTSDPGGIPGFALELLPIVDGARY
                     ATAEASACENLTGFDPAAADAEFDILGTFSSLAPGAYPNHRLVINNRGAGTTHDAYAD
                     AVSATAPLVITPAGTTITVALNPVGACPSNSKHHVTLSPAHQFRVESPRDRLFVITTP
                     VTYLCNSTAGVRTLKRYSGYAIQSAQPATEAAIGVAGALVADRLSACSVDTDTTSVQD
                     RGLVTITLTLEEQGEQIRLIHQVQLDNSR"
     gene            complement(2937..3476)
                     /locus_tag="AC158Q_05135"
     CDS             complement(2937..3476)
                     /locus_tag="AC158Q_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HIR3339190.1"
                     /translation="MRRFHRERGVSLAELIVSIVVVGVGVAGILSVMNLTTRHSADPM
                     IQQQAQLIAESYLEEILLKKFYDPETNTVCAGTTAGESRTSYDNVCDYNGVNDNTGAR
                     NQFGTVVAGLENYNVVVTVTGDGTVALGSLNNTGAVRVLKVDVVVTHDNFSGISVPLT
                     GYRANYNCNAPADAGCKPL"
     gene            complement(3473..3943)
                     /locus_tag="AC158Q_05140"
     CDS             complement(3473..3943)
                     /locus_tag="AC158Q_05140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly FimT family protein"
                     /protein_id="HIR3339191.1"
                     /translation="MERRLDHRGFTQVELIVVIVIAGLLAAVAIPRFVDQQTFQERGF
                     YDEALSAARYAQKFAVTSGCEVQLSVAGGVYVLNQRATDCTTGGFTRLVEHPGTRAAS
                     FTGTTPDGITFTMSTGAVVFDGLGKTADGLSRTVTVGSRSFEIVGATGFVREGP"
BASE COUNT          694 a         1297 c         1355 g          792 t
ORIGIN      
        1 gcagcagtgc aggtgccgtt gagcgtcgaa ccctttagcg tccagtagcg gttgatgctc
       61 ttggctgcgt cgatgcccga tgtctcccct gtcgtatcgg gatgatcggc ggccggcgag
      121 cccggcgtcg ctaccgtgag actgccggtg gtaggcgctg ccgggaaagt gatcgttacc
      181 ggcgtgaagt tcgtcccgtc gccgacggca taggtgaagg aagtgcccgc ggtggcggga
      241 aacacccttg cgaggttccc caccacgtgc tgcgatgggg cggcccccga aatactgccc
      301 gccgactcaa tgctcagcgt gttggcggcg gtggtgttca aatttccgct aatgaaagtc
      361 agtgtgccat tgacgatcgg gctgctggcc agggtgatgc cattgctgtt gttgatggtg
      421 agatccagga gctgcaccac gccgctcggg atacttacgc cggccgagat gcccccgaat
      481 tccagactgg aagaggcagt cgtcgacatg ttggaggtcg tgccggctgt gttgggtccg
      541 ttcagggtca gcttgttcgc cccgaccgcg aacgtgccgc tggtcagcgt gagcgtgccg
      601 ctgagggtgg gactgccgcc gagcgtcgcg ccgttggcat cgttcaatat caggttgcca
      661 taggtgccgg cacccgtcag ggaatggacg gccgcaccgc cgctcaacgt gatgttcccg
      721 gtgctgacgg tgtgcgtccc ggtgttgctt acgtttccgt tcacggtatg ggtgaacgtg
      781 tctccggcga aggttacgct gctgatcgac aggtctcccc tgaccgtaaa tgtgcccaat
      841 gcggttttgg tcgagctgcc gctcaaggtg agatggccgt agccgggcgc gcccacggcg
      901 ttcgccacgt tctgggcggt gccgttgtag ttgacggtgc tggtggcgcc cagcgttgca
      961 gttgtgtagt tggcaggaaa cgtattggtg ccgccgatta tcagcgtcgc gccagtgccg
     1021 acagtgatgg ttccaccggc ggccgtcctg ttcgcggtga atgtcgcaag atccagtgtt
     1081 ccggcggtga gagtaaggtc gccggccacc tgtgcgttgc tggcaaacgt cgccgtgccg
     1141 cccgccttgt tgatcgtgag attggtgtag ctatgcacgc cgccgccgat gacctgggcg
     1201 cccgtgccgt tatacgtaac ggtcgaagtg ctggtgagcg tgctgaccgt catatcgccc
     1261 accaccgtaa aaggactggc accgccggtc agggagccgg cgcccgtcac tgtgccggtg
     1321 accgagaggc tctgccctgc ggcccctgcg acgctcgctc ccgactgaat ggtgaggctc
     1381 aggatcgtcg ggctggtatt cagcacggga aacccggtgc cgttgtcggg aaccgtggga
     1441 atggtgacgt catctccggc agccggcact ccgttgggtg cccagttttg cgcacggttc
     1501 caattcgtag cgttcgcgcc gccgccgcta cgccatgtct tcgcaactgc ccaggccggc
     1561 tcgctggcaa cggcgaacgt cgcaaacgcg atcagcactg tccagctctt gatcatgccg
     1621 ttattcatgg aatgttcgtc accgtcgcct gcattcgccg ccgcgcgtaa ttgatgctgc
     1681 cgaacgcgcc tcgttccgcc gtgctggtga tgacatacac attgccgccg gtgtgggtca
     1741 ggccgccggt ggtacaggtt actgtggtgg tcacactgct caatccattc cccgtaaggg
     1801 taagtggggt ggaacccgcg caactgcccg ccgcaatggc ttgctgtatc ccccaatcga
     1861 gcccggcctt ggcgccgtag tacacgcgcg tcgcgtcgag cgcctgggta ggcgtcgtct
     1921 ggtgtacccc gctcagggtg accatgaagg cccccagcgc cgccagcacc accagcagga
     1981 agatcgccgt gacgagggca aagcctgaat ccgccttttt cttcatcggc tgttgtccag
     2041 ttgcacctgg tgaatgagcc gaatctgctc accctgctcc tccagcgtca gcgtaatagt
     2101 cacaagccct ctgtcctgca cgcttgtggt gtcggtatcc acgctgcagg cgctcagccg
     2161 atcagccacc agcgcgcctg ccacaccgat cgcggcttcc gtcgcgggct gtgccgactg
     2221 aatggcgtat cccgaataac gtttgagcgt gcgcacgccg gcggtcgagt tgcacagata
     2281 ggtcaccgga gtggtaatca cgaacaggcg gtcgcgcggg ctttccactc ggaactggtg
     2341 ggcagggctc agcgtgacgt ggtgcttgct gttggacggg caggcgccca cgggattcag
     2401 ggcaacggtg atggttgtcc ccgccggagt gattaccagc ggagcggtgg cgctcacggc
     2461 atccgcataa gcatcgtggg tggtgcctgc gccccgattg ttgatcacca ggcggtgatt
     2521 cggataagcg cctggcgcca acgaagaaaa agtaccgagg atgtcaaact cggcatcggc
     2581 cgcggcaggg tcaaaccccg tcagattctc gcaggcactt gcctccgcgg tggcatatct
     2641 cgcaccgtcc acaatgggca gcaattccag tgcgaatccg ggaatcccgc ccggatcgct
     2701 ggtcacgcgc acgctgttgg gcagcgcgat gcggatatca cgcgccatgc gccgcagcgc
     2761 gctttcggcg gaatccacca gcgaagcgcg ccggctgaga tccacgtagc cctggaccgg
     2821 ccgcagcacg aataccgcca ccatgcccgc cacgatggag gtgatggtga tcaccacgac
     2881 gagctccacc agggtaacgc cccgggtatt gcgccgcagt cgaaatgccc ctgggatcac
     2941 agtggcttgc aacccgcgtc ggcgggcgcg ttgcagttgt agttcgcgcg gtagccggta
     3001 agcggaacgc tgatgccgga aaagttgtcg tgggtcacga ccacgtccac cttcagcacg
     3061 cgcaccgccc ccgtgttgtt gaggctgccg agcgcgaccg tgccgtctcc ggttacggtg
     3121 acgaccacgt tatagttctc caggccggcc accacggtcc cgaactggtt gcgcgctccg
     3181 gtgttatcgt tgacgccgtt gtagtcgcag acgttgtcgt agctggtgcg gctttctccc
     3241 gccgtggtac cggcgcagac ggtattggtt tccgggtcgt agaacttctt cagcaggatc
     3301 tcctccaggt aactctcggc gatgagctgg gcctgctgct ggatcatggg atcggcgctg
     3361 tggcgggtcg tgagattcat gacggaaaga atgcccgcga cgcccacgcc cacgacgacg
     3421 atggaaacga tcagttccgc caggctgacg ccgcgttcgc ggtgaaaccg cctcacggcc
     3481 cctcccggac gaagccggtg gccccgacga tctcgaagga cctgctgccc acggtcacgg
     3541 tccgcgagag cccgtccgcg gtttttccga gcccatcgaa taccaccgcg ccggtggaca
     3601 tcgtgaacgt gatgccgtcg ggcgtggtgc cggtaaaact cgccgcgcgg gttccgggat
     3661 gctcgaccag tcgagtgaat cctcccgtgg tgcagtcggt ggcgcgctga ttgaggacgt
     3721 agacgccgcc ggctaccgac aactgcacct cgcaaccgct ggtcaccgcg aacttctgcg
     3781 cgtagcgcgc ggcggagagc gcctcgtcgt agaacccgcg ttcctggaaa gtctgctggt
     3841 cgacgaaacg cgggatggcg accgcggcga gcaggccggc gataacgata acgacgatca
     3901 actccacctg agtgaaaccc cggtgatcga gtcgccgttc cattatcgcg ccctatcgat
     3961 tgtccgccgg attcgccggc ggagccgagg tctcccagcc gcacccagct gccgcgcagg
     4021 ctccacgagc ggcgccgcag cgagcgccgg gggtaaaacg aacagcgccc cgattacaac
     4081 cgaccgggga gcggctggtc ccgggacctc agcgacgact aagttgcagt tcgaaaca
//
LOCUS       DBNGSG010000195         6529 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3290530, whole genome shotgun sequence.
ACCESSION   DBNGSG010000195 DBNGSG010000000
VERSION     DBNGSG010000195.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6529)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6529)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6529
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3290530"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1362)
                     /gene="dnaB"
                     /locus_tag="AC158Q_05145"
     CDS             complement(<1..1362)
                     /gene="dnaB"
                     /locus_tag="AC158Q_05145"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HIR3339192.1"
                     /translation="MAQAQVVTSIVKDAQVEAARVPPQSVEAEQSVLGGLLLDNAAWD
                     RVADLITEQDFYRSDHRLIFQHIGSLIEHGKPADTLTVAESLERAGKLVEVGGNAYLG
                     SLSLNTPSAANIRRYAEIVRERSIMRSLATVGTEIADSAYSPTGRDAKALVDEAESKV
                     FQIAEAGSKSKQGFVKIDPLLTETVERIDMLYNRENKSDIIGLATGFVDLDRMTSGLQ
                     QGELVVVAARPSMGKTALAMNIVEHVALDLKKAVAVFSMEMSGTQLAMRMIGSVGRFD
                     QHELRTGTFKDEDWSRMVESVGKLNEAQLYIDDSAGLNALEVRSRARRLHRQCGGLSL
                     IVVDYLQLMSASSGREENRATEIAEISRSLKSLAKELKLPVVALSQLNRSVESRQDKR
                     PMMSDLRESGAIEQDADLILFIYRDEVYNPGNTEKHGEAEIIIAKQRNGPTGKIDLTF
                     LGKH"
     gene            complement(1512..1961)
                     /gene="rplI"
                     /locus_tag="AC158Q_05150"
     CDS             complement(1512..1961)
                     /gene="rplI"
                     /locus_tag="AC158Q_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HIR3339193.1"
                     /translation="MQVILLEKIVNLGSLGDVVKVKEGFARNYLIPNGKARRATPQNL
                     ADFEKRRTQLERAQNEALARAQEQAAKLDGLMVQITQKAGVDGKLFGSVTNVDISDAL
                     KGQGLEVPKAMIRMPQGPLKQVGDHPIKIALHADVVVTVTVSVLGEQ"
     gene            complement(1972..2301)
                     /gene="rpsR"
                     /locus_tag="AC158Q_05155"
     CDS             complement(1972..2301)
                     /gene="rpsR"
                     /locus_tag="AC158Q_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HIR3339194.1"
                     /translation="MQRQGYGGGGGARGRGKGKGKGKFAKGKDKDKRGGRNQLFRRRK
                     FCRFTADGTKEIDYKDIGILKEFINENGKIIPARITGTKARYQRQLGIAIQRARFLAL
                     LPYTDLH"
     gene            complement(2311..2613)
                     /gene="priB"
                     /locus_tag="AC158Q_05160"
     CDS             complement(2311..2613)
                     /gene="priB"
                     /locus_tag="AC158Q_05160"
                     /inference="COORDINATES: protein motif:HMM:TIGR04418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HIR3339195.1"
                     /translation="MEENEVALSGELGAIEPLRYTPAGIPLLSFKLMHKSRQTEAGLK
                     RQVECEMGAVAMAEVAVAMARLKPGQVVKVSGFLNRKNRMSSQLILHVTEARAIKE"
     gene            complement(2616..3020)
                     /gene="rpsF"
                     /locus_tag="AC158Q_05165"
     CDS             complement(2616..3020)
                     /gene="rpsF"
                     /locus_tag="AC158Q_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HIR3339196.1"
                     /translation="MRHYEVVFIVHPDQSEQVPGMVERYRQMVTGRNGKIHRLEDWGR
                     RQLTYPIQKVHKAHYVLMNVECDNETLGELEHAFKFNDAVLRHLMVKMSGPVTAPSPM
                     MKEEKSRSLTSPAAEESPRAATPPAPAEEAAV"
     gene            3318..3677
                     /locus_tag="AC158Q_05170"
     CDS             3318..3677
                     /locus_tag="AC158Q_05170"
                     /inference="COORDINATES: protein motif:HMM:NF016370.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HIR3339197.1"
                     /translation="MRLNNLKWLQSRRRYLRASLTPAEARLWNYLKISRLSGRKFRRQ
                     HSMGRYILDFYCPSEKLAIELDGATHDRDIAQRRDRTRDRFLQNAGIRILRIQNDDVI
                     KDLDGVLSQIRRHLATP"
     gene            complement(3721..4806)
                     /locus_tag="AC158Q_05175"
     CDS             complement(3721..4806)
                     /locus_tag="AC158Q_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339198.1"
                     /translation="MGVEIKLTDKEFPVSPVFVDFLHRQIEGHSHEATWHDQLSEDLV
                     PAQADEMQKAAVAVAEQVLQDPVGQKAILRSYELLTALLTGQADKLRQFHQRYRFICV
                     VGCPRHGGTYLTKHLFLAAGLVPEQVPNAIAHDGFPDAAPFQFKENYSSLTSMIQNMA
                     EYLAMVEVFYANSRLFDNRLVVPKKATKAVYHGAFFHTALGPDTEYVITLRHPLAACI
                     STYEKSTGLPADGKFKVRGNIEEWALRDCVFTGADPKEILKQDYFDVYLRYWEQYHNN
                     LALTGLAANRNRTVVAYGRERLAGLARGYFKRFQTGGQPEDFKVFDKRGRHPQWQNRA
                     DAALRRVAENWKSVGLAFPVDELMEGW"
     gene            complement(5078..>6529)
                     /locus_tag="AC158Q_05180"
     CDS             complement(5078..>6529)
                     /locus_tag="AC158Q_05180"
                     /inference="COORDINATES: protein motif:HMM:NF015803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HIR3339199.1"
                     /translation="ALSNEEMEQLVAPHTGQNKDFADIQRALEALEQAFRDRGYGVAQ
                     VILPEQDITRGVVQFRIVQPRVGRVVIEGNTRFDAENIRRSLPTVKEGETPNSKDIAR
                     NLQITGEHPVKQTNVLLRAGASEDQVDVAIKVTDDKPWRAFFTLDNTGTGETGYFRTG
                     FGVQHTNLFNRDHTLTASYITSPGHVSDVTIFGLGYRMPFYDLNSSLDLIAGYSDVNS
                     GVVQGLFNVSGSGSIFAARWNYFLHKWKDMEQKLTFGLDYRAFKNEVLVAGQPLVPDI
                     TVRPASVTYSGLYRMTAAELSFYAGAAANIPGGNDGDQAAFTASRPGANDDYTMLRYG
                     MNYTRQFRNEWQTRFGFNGQDTSNALVAGEQYGIGGPDTVRGYLLREVANDKGYSGQV
                     ELYTPDLARGFGMTDAYKTRLLAFYDWGSVERNHALPGEIQRDSIASVGVGLRLTYKK
                     SVSLRLDLAQILQETANRDNDSQRVTGALAIVF"
BASE COUNT         1065 a         2100 c         2004 g         1360 t
ORIGIN      
        1 gtgcttgccg aggaaggtga ggtcgatttt gccggtgggg ccgttgcgct gcttggcgat
       61 gatgatttcg gcctcaccgt gcttttcggt attgccgggg ttgtagacct cgtcgcggta
      121 gatgaagagg atcaggtcgg cgtcctgctc gatcgcgccg gactcgcgca ggtccgacat
      181 catcgggcgc ttgtcctggc gcgactccac gctgcggttc agctgcgaga gcgcgaccac
      241 cggcagcttc aattccttgg caaggctctt cagcgagcgt gaaatctcgg cgatctccgt
      301 ggcgcggttc tcctcccggc cgctgctcgc ggacatgagt tgcaggtagt ccaccacgat
      361 cagcgacagc ccgccgcact gccggtgcag gcgccgcgcg cgcgagcgca cttccagcgc
      421 gttcagcccc gcgctgtcgt ctatgtagag ttgcgcctcg ttcagcttgc ccaccgattc
      481 caccatccgc gaccagtcct cgtccttgaa ggtcccggtg cgtaactcgt gctggtcgaa
      541 gcggcccacc gagccgatca tgcgcatcgc gagctgggtc cccgacattt ccatgctgaa
      601 caccgcgacc gcctttttca ggtcgagcgc cacgtgctcc acgatgttca tggcgagggc
      661 tgttttcccc attgacggtc tagccgcgac gaccacgagc tcgccctgct gcaggcccga
      721 ggtcatgcgg tcgagatcca cgaacccggt ggcgagaccg atgatgtcgc tcttgttctc
      781 gcggttgtag agcatatcga tgcgctcgac cgtctcggtg agcagcggat cgatcttgac
      841 gaacccctgt ttcgacttgg agcccgcctc cgcgatctgg aacaccttcg actccgcctc
      901 gtccaccagc gccttggcgt cccgccccgt cgggctgtag gcggagtccg cgatctcggt
      961 ccccaccgtc gccaggctgc gcatgatgga gcgctcgcgc acgatctccg cgtagcggcg
     1021 gatattggcc gcgctcgggg tgttcagcga gagcgagccg aggtaggcgt tcccccccac
     1081 ttcgacgagc ttgccggcgc gctcgaggct ctcggcaacg gtcagggtat cggccggctt
     1141 gccgtgctcg atgaggctgc cgatgtgctg gaagatgagg cggtgatccg agcggtagaa
     1201 atcctgctcg gtgatgaggt ccgccacccg gtcccacgcg gcattgtcga gcagcagccc
     1261 gccgaggacc gactgctcgg cctcgaccga ctggggagga acccgcgcgg cctcgacctg
     1321 ggcatccttg acgatcgagg tgacgacctg cgcctgggcc atgatgcgtt gtgcggagtg
     1381 ctgggttgtt tgagtttgtg gtcgctggct gccgacactg gccggagaag cactgccgga
     1441 gaagtatacg aaaaaaaagg actgtcgcca gtcctttttt cacgcaaaaa aatttgctgt
     1501 gttatcagga cttactgttc ccccagtacc gaaacggtga ccgtgacgac gacgtccgcg
     1561 tgcagcgcga tcttgatcgg atggtcgccc acctgcttca gcgggccctg cggcatgcgg
     1621 atcatcgctt tcggcacctc caggccctgc cccttcagcg cgtcggagat gtccacgttg
     1681 gtgaccgagc cgaagagctt gccgtccacg ccggctttct gcgtgatctg caccatcagc
     1741 ccgtcgagct tggccgcctg ttcctgggcc ctggcgagcg cctcgttttg cgcccgctcc
     1801 agctgggtgc ggcgcttttc gaaatcggcc aggttctggg gcgtggcccg ccgcgccttg
     1861 ccgttcggga tcaggtagtt gcgggcgaac ccttccttca ccttgacgac gtcgccgagg
     1921 ctgcccaggt tcacgatctt ttccagcagg atgacttgca ttgcggcgct cctagtgcag
     1981 atcggtgtac ggcagcagcg cgagaaaccg cgcgcgctgg atcgcgatgc ccagctggcg
     2041 ctggtagcgc gccttggtgc cggtgatgcg cgccgggatg atcttgccgt tctcgttgat
     2101 gaattccttg agtatcccga tgtccttgta gtcgatttcc ttggtgccgt cggcggtgaa
     2161 gcggcagaac ttcctgcgcc ggaacagctg gttgcggccg ccgcgcttgt ccttgtcctt
     2221 gcctttcgcg aacttgccct tgcctttgcc cttgccgcgg ccgcgtgccc cgccgccgcc
     2281 accgtagcct tgtctttgca ttgccatgat ttattccttt atcgcccgtg cttcggttac
     2341 atgcaatatc agttgcgagc tcattcggtt cttccggttc aggaaaccgc tcaccttcac
     2401 cacctggccc ggtttcagcc gggccatcgc caccgccacc tccgccatcg ccaccgcgcc
     2461 catttcgcac tcgacctggc gtttcaatcc ggcctccgtc tggcgcgact tgtgcatcaa
     2521 cttgaaactg agcagcggaa tgccggcggg cgtgtaacgc agcggctcga tggcgccgag
     2581 ctcgccgctc aacgcgacct cgttctcttc cactcttaca ctgccgcctc ttccgccggt
     2641 gccggcggcg tagccgccct ggggctctcc tccgcggccg gcgaagtgag cgaccgggac
     2701 ttctcttcct tcatcatcgg cgagggcgcc gtcaccggcc cgctcatctt taccatcagg
     2761 tggcgcagca ccgcgtcgtt gaacttgaag gcgtgttcga gctcgcccag cgtctcgttg
     2821 tcgcactcga cgttcatgag aacgtagtgc gccttgtgga ctttctggat cggataggtc
     2881 agctggcgcc ggccccagtc ttccagccgg tggatcttgc cattgcgccc cgtgaccatc
     2941 tggcggtagc gctcgaccat gccggggacc tgctcgctct ggtccggatg gacgatgaaa
     3001 accacttcgt aatgccgcat cggtacgact ccttgtgggt taagggctct gggagccccc
     3061 agcctcccgt cttgctgccg cggtgaggca aggaaccccc tcccaaccct cccccttaac
     3121 aaggtggagg actttctttt cccttccccc tatccagggg aaagagtcag ggcgggggtg
     3181 aaacgaaggg gcggattata cagaagctag cttgaaaatc aaagggtttc gccacccctc
     3241 gcgtacgctc accccctcct acgccaggag gggttggttg cgtcagcagc cgaattggtt
     3301 aaagcgttta tgaatccatg cgcctaaata acctcaagtg gctccagtcc cgtcgccgtt
     3361 acctccgggc cagcctgact cccgctgaag cacgcctttg gaattatcta aagatcagtc
     3421 ggttatcagg caggaaattt cgtcgtcaac acagcatggg gcgttacata ttggatttct
     3481 actgcccaag tgaaaagttg gcaatcgaat tggacggtgc gacacacgac cgtgatatcg
     3541 cgcagcgtcg tgatcgaaca cgagacaggt ttctacagaa cgcgggaatc cgcatcctgc
     3601 gaattcagaa cgacgatgtg attaaagatc ttgatggagt gttgtcgcaa atccgtaggc
     3661 acctcgccac cccttgatcc tctcctcgtc aggtaggggt agcgcgcagc gccggggtgg
     3721 tcaccagcct tccatgagtt cgtcgaccgg aaacgccagc cccacggatt tccagttttc
     3781 cgccacgcgc cgcagggcgg catcggcccg gttctgccat tgagggtgcc gcccgcgctt
     3841 gtcgaacacc ttgaaatcct cgggctgccc cccggtctgg aagcgcttga aatagcccct
     3901 ggcgagaccg gccaggcgct cgcgcccgta cgccaccacc gtccggttgc ggttcgcggc
     3961 aaggccggtc agcgcaagat tgttgtggta ttgctcccag taacgcagat aaacatcgaa
     4021 atagtcctgt ttcaggatct ccttcggatc ggcgccggtg aacacgcagt cgcgcagcgc
     4081 ccattcctcg atgttgccgc gcaccttgaa ctttccgtcc gccggcagcc cggtggattt
     4141 ttcgtaggtc gagatgcagg cggccagtgg gtggcgcagc gtgatcacgt actcggtatc
     4201 gggccccagc gccgtatgga aaaatgcgcc atgataaacg gccttggtcg cctttttcgg
     4261 caccacgagc cggttgtcga agaggcgcga gttggcgtaa aaaacctcca ccatcgccag
     4321 gtactccgcc atgttctgga tcatggaagt gagggagctg tagttctcct tgaactggaa
     4381 tggcgccgcg tccggaaaac catcgtgcgc aatggcattc ggcacctgct cggggaccag
     4441 cccggccgcc aggaacaagt gcttggtgag ataggtgccg ccgtggcgcg ggcatcccac
     4501 cacgcaaatg aagcgatagc gctggtggaa ctgcctcagt ttgtccgcct gcccggtcaa
     4561 aagcgcggtc agcagctcgt agctccgcag gatcgccttc tgccccaccg gatcctgcag
     4621 cacctgttct gccacagcga ccgccgcctt ctgcatctcg tcggcctgcg ccgggaccag
     4681 atcctcggac agctggtcgt gccaggtggc ttcgtgggag tggccctcga tctggcggtg
     4741 cagaaagtcg acgaataccg gtgagaccgg gaattccttg tcggtcagct tgatttctac
     4801 gcccatgtca ttctctgccg ggtcgcgaat gtctgcccga aaatgaggag agaggtttcg
     4861 ctcaatcccc tctttaacca cccctcgctt acgctcttcc cctcaaccag gcgggaaagt
     4921 tcacctgcgc tggtcccctc ctggtccagg aggggtggct gcgccagcag ccggggtggc
     4981 cttcccctcc tagtttagga ggggtggctg cgccagcggc cggggtggcc ctcccctcct
     5041 agtttaggag gggcggctgc gccagcggcc ggggtggtta gaagacgatc gcgagcgcgc
     5101 cggtcacgcg ctggctgtcg ttatcgcggt tggcggtttc ctgcaggatc tgcgcgagat
     5161 cgaggcgcag gctcaccgac ttcttgtagg tcagccgcag cccgactccc acactggcga
     5221 tcgagtcgcg ctgaatttcg ccaggcagcg cgtggttgcg ctcgaccgaa ccccaatcgt
     5281 aaaaggccag cagccgggtc ttataagcgt ccgtcattcc gaatccacgc gcgagatccg
     5341 gggtgtagag ctcgacctgg cccgaatagc ctttgtcgtt tgcgacttcg cgcagcaagt
     5401 atccgcgcac cgtatccggc ccgccgatgc cgtactgctc gcccgcgacc agcgcgttgc
     5461 tggtgtcttg cccgttgaac ccgaatcgcg tctgccactc gttgcgaaac tggcgggtgt
     5521 agttcatgcc gtagcggagc atcgtgtagt cgtcatttgc gccgggccgc gaggcggtga
     5581 acgccgcctg gtcgccgtcg ttgccgcccg ggatgttcgc cgccgcgccg gcataaaacg
     5641 acagttccgc ggccgtcatg cggtacaggc cgctgtaggt cacgctcgcc ggccgtaccg
     5701 tgatgtccgg caccagcggc tggcccgcca ccaggacttc attcttgaac gcgcgataat
     5761 cgagaccgaa agtgagcttc tgctccatat ccttccactt gtgcaggaag tagttccagc
     5821 gcgcggcaaa gatggagccg ctgccgctca cgttgaacaa cccctgcacc acgcccgagt
     5881 tcacatcgga gtaacccgca atgaggtcca gcgagctgtt gaggtcatag aacggcatgc
     5941 ggtagcccag gccgaagatg gtgacgtcgc tcacgtggcc cggtgacgtg atgtaactcg
     6001 cggtcagcgt gtgatcgcgg ttgaacaggt tggtgtgctg cacgccgaaa ccggtccgga
     6061 aatagccggt ttcgccggtg cccgtattgt cgagggtgaa gaaagcgcgc cacggcttgt
     6121 catcggtaac cttgatggcg acgtccacct ggtcctcgga tgcgcccgcg cgcagcagca
     6181 cgttggtctg cttgaccgga tgctcgccgg tgatctgcag gttgcgcgcg atgtccttcg
     6241 aattgggggt ctcgccttcc ttcaccgtcg gcaggctgcg ccggatgttc tccgcgtcaa
     6301 accgggtgtt gccttcgatc actacccggc ccacgcgcgg ctggacgatg cgaaattgca
     6361 ccacgccgcg ggtgatgtcc tgctcgggca gtatgacctg cgcgacgccg tagccgcggt
     6421 cgcggaaggc ctgctccagc gcctccagcg cgcgctgaat gtcggcgaag tccttgttct
     6481 ggccggtgtg cggcgcgacc agttgttcca tctcctcatt ggatagcgc
//
LOCUS       DBNGSG010000196         1898 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1931954, whole genome shotgun sequence.
ACCESSION   DBNGSG010000196 DBNGSG010000000
VERSION     DBNGSG010000196.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1931954"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="AC158Q_05185"
     CDS             complement(<1..183)
                     /locus_tag="AC158Q_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339200.1"
                     /translation="MPDIARETPQRRGFSLGGVNGEWRVEPDVPGTPVTDHALLSQQG
                     KDFVAEALARLVVERLD"
     gene            complement(200..1075)
                     /locus_tag="AC158Q_05190"
     CDS             complement(200..1075)
                     /locus_tag="AC158Q_05190"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3339201.1"
                     /translation="MRILVPFPPGGGTDIQARLLSTAFQKSMGQNFLIENRTGASGLI
                     ATQATVDSPPDGYTILFTSGSIAPVTTLFAKRIKFDMHKDLQPISLLSSTPLVLSLHP
                     SVPVTSVKELIALAKRTKGMNVGGNTAGTTAHLAAEMFNQRFGLSSTVVTFRGAGPAV
                     ISLISGEIDYIFATAPSIMPHIRSGRAKAFAVTTKTRSSALPDLPTMDSMYPGFVSYN
                     WYALFYSKGTPRPLVDKMNSEVKKALGTPEIKAFYPREALDPVGGSPEELAVFFKDEV
                     EKYAKVIKSANIRVE"
     gene            complement(1407..>1898)
                     /locus_tag="AC158Q_05195"
     CDS             complement(1407..>1898)
                     /locus_tag="AC158Q_05195"
                     /inference="COORDINATES: protein motif:HMM:NF024375.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3339202.1"
                     /translation="AKKSPAASADDYDHTFKAIYDHEIPWESDDVRGLLTLPPGVQVK
                     VMNYDPVMKRLDMKQRFPPGYVEPAHTHKSWHSILVTKGRMCVAGKDLRPGDYVFGWD
                     IPHGPYEYPDGCEVFIVYMGDASHVWEKKDLLQHKNIWKPETEVGKKGVEAHIEKRKA
                     GKV"
BASE COUNT          324 a          595 c          586 g          393 t
ORIGIN      
        1 gtcaagccgc tccacgacga ggcgcgcaag cgcttcggcg acgaagtctt tgccctgctg
       61 ggacaataaa gcgtgatcag tgacaggcgt ccccggcacg tcgggttcca ctcgccattc
      121 accattcacg cctcccaaag aaaaaccccg ccgctgcggg gtttcccggg cgatatcggg
      181 caatggagcg cccgccagct cattcgaccc ggatattcgc cgacttgatc accttggcgt
      241 acttctcgac ctcgtccttg aaaaataccg ccagttcctc ggggctgccg cccaccggat
      301 ccagggcctc cctgggatag aacgccttga tctcgggggt gccaagggcc ttcttgacct
      361 ccgagttcat tttgtcaacg agtggcctgg gcgtgccctt cgagtagaac agcgcgtacc
      421 agttgtagga cacgaagccg gggtacatcg agtccatcgt gggcaggtcc ggtagcgccg
      481 aggaccgcgt cttggtcgtg accgcgaatg ccttggcccg gccgctgcgg atgtgcggca
      541 tgatcgacgg cgcagtggcg aagatgtagt cgatctcgcc cgagatcaac gagatcaccg
      601 ccggcccggc gccccggaag gtgaccaccg tggagctgag gccgaacctc tggttgaaca
      661 tttccgccgc gaggtgcgcg gtggtgcccg cggtattgcc gccgacgttc atgcccttcg
      721 tgcgcttcgc tagcgcgatg agctccttca ccgatgttac cggcacgctg ggatgcagcg
      781 agagcacgag cggcgtcgag gacaacaagc tgatcggctg gagatccttg tgcatgtcga
      841 acttgatgcg cttggcaaaa agggtcgtga ccggggcgat gctgcccgac gtgaagagta
      901 tggtgtagcc gtcgggcggg gaatccacgg tggcctgggt tgcgatcagg cccgaagccc
      961 cggtccggtt ctcgatgagg aagttctggc ccatgctctt ctggaacgcg gtggataaca
     1021 gccgcgcctg gatatcggtc ccgccgcccg gcgggaatgg caccaggatg cgcaccggct
     1081 tttccggcca ctgctgggca atagattgaa gcggaaggga tagcccggtt gttgcgactg
     1141 cggctgcgac tgcgagtgca catgccttat atttcatctg cacctcctcg ttgtaggttt
     1201 tgggtttgac cttgccagtc caccaatatg ctagcacctg gcgctatcgc gcttcctgat
     1261 ttctacaccg gtcctggtgt cgtttacccg gccgcaggcg acaaaaaacc ccaccgcagc
     1321 ggggtttttt gtcgcaacac ggctggatgg gtgcgaccgg taacggaggg gtcacgctta
     1381 cgcgaattgc ccctcatccc tcatcatcag accttcccgg ccttgcgctt ttcgatgtgc
     1441 gcctcgacgc ccttctttcc gacctcggtt tccggcttcc agatgttctt gtgctgcagg
     1501 aggtccttct tctcccacac gtgcgaggcg tcgcccatgt aaacgatgaa cacctcgcag
     1561 ccgtccggat attcataggg accgtgggga atgtcccagc cgaacacgta gtcgcccggg
     1621 cgcaggtcct tgccggcgac gcacatgcgg cccttggtca ccaggatcga gtgccagctc
     1681 ttgtgcgtat gcgccggctc gacgtatccg ggcgggaagc gctgcttcat gtccagccgc
     1741 ttcatcaccg ggtcatagtt catgaccttg acctgcaccc cgggcgggag cgtcagcagc
     1801 ccgcgcacgt cgtcgctctc ccacgggatt tcgtggtcgt agatcgcctt gaaagtatgg
     1861 tcgtaatcgt ccgccgacgc ggccggggac tttttcgc
//
LOCUS       DBNGSG010000197         3640 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3800272, whole genome shotgun sequence.
ACCESSION   DBNGSG010000197 DBNGSG010000000
VERSION     DBNGSG010000197.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3800272"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1650)
                     /locus_tag="AC158Q_05200"
     CDS             complement(<1..1650)
                     /locus_tag="AC158Q_05200"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HIR3339203.1"
                     /translation="MAKRYDESSVKKLRGIEPVQRLPGMYTRTSDPTHIIQEAIDNAA
                     DEALGGHADKIEVTVHQDGSVTVTDNGRGIPIGIHPEEKIPTVELVLTELHAGAKFSR
                     TDAEAAYKFSGGLHGVGVSVTNALSEKLEVEVKRDGGVHTIAFADGAVVKKLKKTGTV
                     GEKNTGTSLRIWPNRKYFDSPKVSLDDLERIVRSKAVLLPGVKVTLNIETAQETKKQT
                     WNYPEGMKGYLTELIGKASAVAPVFDGEKYVSEADSNGFHAGEGAGWAFAFVDDDKGY
                     GESYANLIPTPAGGTHEAGLREGIFEAIKAFVEYHNLMPKGVKLLADDAWARTVYFLS
                     AKVLEPQFHGQTKEKLSNRDAVKLVADMVRDPFALWMNGNVEHGKKIADLVIRQAIER
                     SRTVQKVERRKSSSVVILPEKLTDCESTDVKDNELFLVEGDSAGGSAKQARDKRYQAI
                     YRMRGKALNSWEVKRELLFSNAEIHDIAVALGVDPHDEKDNPDLSGLRYGKVASMTDA
                     DVDGAHISVLLLTLFYRHFPKLIEKGHIFIACPPLYKVDVPG"
     gene            1861..2055
                     /locus_tag="AC158Q_05205"
     CDS             1861..2055
                     /locus_tag="AC158Q_05205"
                     /inference="COORDINATES: protein motif:HMM:NF022894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin MazE family protein"
                     /protein_id="HIR3339204.1"
                     /translation="MKGYRARKQASGMRLIQLWVPDTRSKRFAAECKRQSRLLRGDPG
                     EVEALEFIERVAAWDDDAPR"
     gene            2036..2365
                     /locus_tag="AC158Q_05210"
     CDS             2036..2365
                     /locus_tag="AC158Q_05210"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:0016075 - rRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HIR3339205.1"
                     /translation="MTMRRGDMVTVAMAGDFGKPRPALVIQSDLFDNLPSVTLCLITS
                     ELHPLPIFRITVDPSPENGLRRISQVQVDKVVTVMRERVGGVIGSLDDATMLKVNRAL
                     AVFVGIA"
     gene            complement(2368..3024)
                     /locus_tag="AC158Q_05215"
     CDS             complement(2368..3024)
                     /locus_tag="AC158Q_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HIR3339206.1"
                     /translation="MARPSRIYVLAGVNGAGKSSIAGAAFRNYGGEYFNPDEAARRLM
                     AANPGMDQAAANSAAWRKGRDLLEKAIREKLDFALETTLGGGTISRMLAQAAAQGAGI
                     YVWYVGLSSPELHIRRVRSRVRMGGHDIPEQAIRRRFEHSRLNLIALLPVLTALRLYD
                     NSTEADPAAGKTPVLAPVLIMEHRKILNPGDLPRAPDWSKPIVAAAIKLSRPEMRDWK
                     "
     gene            complement(3100..3513)
                     /locus_tag="AC158Q_05220"
     CDS             complement(3100..3513)
                     /locus_tag="AC158Q_05220"
                     /inference="COORDINATES: protein motif:HMM:NF014643.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HIR3339207.1"
                     /translation="MPNPLTFRNSQGDLVDVPAVAATRLKNEFGSVLEQALRGGAVAI
                     TRHDTPRAVLVSFEEFQSLVKARSPSLNDLGAEYDVLLARMQTPKAKKGMETAFNASP
                     AELGRAAVKATRASEGERRKPAPAGIRRGAKGGGS"
BASE COUNT          594 a         1157 c         1177 g          712 t
ORIGIN      
        1 gccggggacg tccaccttgt agagcggcgg gcaggcgatg aagatgtggc ctttctcgat
       61 cagcttcggg aagtgccggt agaagagggt gaggagcaac acggaaatgt gcgcgccgtc
      121 cacgtccgcg tccgtcatcg aggcgacctt gccgtagcgc agccccgaga ggtcggggtt
      181 gtccttctcg tcgtgcgggt ccacgccgag ggcgaccgcg atgtcgtgga tctcggcgtt
      241 ggagaacagc agctcgcgct tcacttccca ggaattcagc gccttgccgc gcatgcggta
      301 gatcgcctgg tagcgcttgt cgcgcgcctg cttggccgag ccgcccgcgg agtcgccctc
      361 gaccaggaac agctcgttgt ccttgacgtc ggtggactcg cagtcggtca gcttctccgg
      421 caggatcacc acgctcgagg atttcctgcg ctcgaccttc tgcaccgtgc gcgagcgctc
      481 gatcgcctgc cggatcacca ggtcggcgat cttcttgccg tgctcgacgt tgccgttcat
      541 ccacagcgcg aacgggtcgc gcaccatgtc cgcgaccagc ttcaccgcgt cgcggttgga
      601 gagcttctcc ttggtctggc cgtggaactg cggctccagc accttcgccg agaggaaata
      661 gacggtgcgc gcccaggcgt cgtcggcaag cagcttcacg cccttcggca tcaggttgtg
      721 gtactcgacg aaagccttga tcgcctcgaa gatgccctcg cgcagaccgg cttcgtgcgt
      781 gccgccggcg ggggtgggga tcaggttcgc gtacgactcg ccgtagccct tgtcgtcgtc
      841 gacgaaagcg aacgcccagc ccgcgccttc gccggcgtga aaaccgttgg aatccgcttc
      901 gctcacgtac ttctcgccgt cgaacaccgg ggcaaccgcg ctggctttgc cgatcagctc
      961 ggtgagatag cccttcatgc cctcggggta gttccaggtc tgcttcttcg tctcctgcgc
     1021 cgtctcgatg ttgagcgtga ccttcacgcc cggcagcagc accgccttgg agcgcacgat
     1081 ccgctcgagg tcgtcgagcg aaactttcgg ggaatcgaaa tacttcctgt tcggccagat
     1141 gcgcagcgac gtgccggtgt tcttctcgcc gaccgtgccg gtcttcttca gcttcttcac
     1201 gaccgcgccg tcggcgaacg cgatcgtgtg cacgccgccg tcgcgcttca cctcgacttc
     1261 gagcttctcc gacagcgcgt tggtcaccga cacgccgacc ccgtgcagcc cgccggagaa
     1321 cttgtaggcg gcttccgcgt cggtgcggga gaatttcgcg ccggcgtgca gttccgtcag
     1381 caccagctcg acggtgggga tcttctcttc cgggtggatg ccgatcggga tgccgcggcc
     1441 gttgtcggtg acggtcaccg agccgtcctg gtgcacggtg acttcgattt tgtcggcgtg
     1501 gccgccgagc gcttcgtcgg cggcgttgtc gatcgcttcc tggatgatgt gggtggggtc
     1561 ggacgtccgg gtatacatcc ccgggagccg ctggaccggc tcgatacctc tcaacttctt
     1621 gactgatgac tcgtcgtacc tttttgccat tcgctcggct atcctcggaa atggggctgc
     1681 gggtatttta tccctttgct aatcaaagta ctagcccctg ggccccagtg acgggtgccg
     1741 ggtgacgagt gacgagcgcc caaaccccct atgagccttg atgagcctgg catccccgcg
     1801 taacatgcat cgtaacatgt tacggggcga atcatgagag ccttgaaggc aaaggaaaga
     1861 atgaagggat accgtgcccg caagcaggcg agcggcatgc gtttgatcca gttgtgggtg
     1921 ccggacacgc gctcgaagcg ctttgcggca gagtgcaagc gccaatcccg gctgctgaga
     1981 ggcgacccag gggaagtcga ggcgctggaa tttatcgagc gtgtggccgc gtgggatgac
     2041 gatgcgccgc ggtgacatgg tgacggttgc catggctggc gactttggca aaccacgtcc
     2101 cgccctggtc atacagtccg acctgttcga caatctgccg agcgtaacgc tatgcctgat
     2161 cacgagcgaa ctccacccgt tgccaatatt tcgcatcacc gtcgatccgt ccccggaaaa
     2221 tgggctgcgc cgtatttccc aggtccaggt ggataaggtg gtcacggtca tgcgcgaacg
     2281 tgttggcggg gtgatcggca gcctcgacga cgccacgatg ctgaaggtca accgcgctct
     2341 cgccgtcttc gtcggcattg cctgacatta cttccagtcc ctcatctccg gcctgctgag
     2401 cttgattgcg gcggccacga tcggcttcga ccagtccggc gcccgcggga ggtcgccggg
     2461 attgagtatc ttgcgatgct ccatgatcag cacgggcgca aggacggggg tcttgccggc
     2521 cgcgggatcg gcttccgtgc tgttgtcgta caggcgcagc gccgtgagca cgggcagcag
     2581 ggcgatcagg ttcaggcggc tgtgctcgaa acgccggcgg atcgcctgct cgggaatgtc
     2641 gtggcctccc atgcgcacgc gggaccgcac gcgccggata tggagctccg ggctggacag
     2701 gccgacgtac cacacgtaaa tcccggcgcc ctgcgcggcg gcttgcgcca gcattcgcga
     2761 aatcgtgccg ccgcccaggg tggtttcgag ggcgaaatcc agtttttcac ggatcgcctt
     2821 ttccagcaga tcccggcctt tccgccaggc ggcgctgttg gccgcggcct ggtccatgcc
     2881 cgggttcgcg gccatcaggc ggcgcgccgc ttcgtcgggg ttgaaatact cgccgccgta
     2941 attgcggaat gccgcgcccg cgatgctgct cttgccggcg ccgttgacgc ccgcgagcac
     3001 gtagattcgc gatggccgcg ccatcgggat tcaaggcgaa agtcgaaagt cgaaagtcga
     3061 aaggaaagac acggcaatct ccaatcgcga gtcgcggctt tagctccctc cgcctttcgc
     3121 cccccgcctg atacctgcgg gggcaggctt ccgcctttcg ccttccgacg cgcgcgtggc
     3181 tttcaccgcg gcgcggccca gctcggccgg cgaggcattg aacgccgttt ccatgccttt
     3241 cttcgccttg ggcgtctgca tgcgcgccag cagcacgtcg tactccgcgc ccaggtcgtt
     3301 caggctcggg gagcgggctt tgacgagaga ctggaactct tcgaaagaga ccaggaccgc
     3361 ccggggggtg tcatgccggg taatggcgac ggccccgccc cgaagagcct gctcgaggac
     3421 cgacccgaac tcgttcttga gccgggtggc ggcgacggcc gggacatcga ccagatcgcc
     3481 ctggctgttg cggaaagtga gtgggttggg catgcggcgc ctcgcgaaat aggggctatt
     3541 atagctaaaa tagctaaaat agccaatact ggactaaagg cacaaggcac aaggcacaag
     3601 gcacaaggca caagggaggc gctgtctaat cccttccctt
//
LOCUS       DBNGSG010000198         5710 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4309491, whole genome shotgun sequence.
ACCESSION   DBNGSG010000198 DBNGSG010000000
VERSION     DBNGSG010000198.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5710)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5710)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5710
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4309491"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="AC158Q_05225"
     CDS             complement(<1..444)
                     /locus_tag="AC158Q_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HIR3339208.1"
                     /translation="MSYWLFKSEPSTYGIDHLARDKVTAWSGVRNYQARNFMRDAMRK
                     GDRGFFYHSSCEVPGIAGILEVAGAAYPDETQFDRKSRQYDPGASKDNPRWFNVDVKF
                     VKKTRVVSLAELRGQKALKNLPVLQRGNRLSITPVTQAEWECIGRL"
     gene            complement(533..711)
                     /gene="ssrS"
                     /locus_tag="AC158Q_05230"
     ncRNA           complement(533..711)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="AC158Q_05230"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(713..1021)
                     /locus_tag="AC158Q_05235"
     CDS             complement(713..1021)
                     /locus_tag="AC158Q_05235"
                     /inference="COORDINATES: protein motif:HMM:NF017016.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0000921 - septin ring assembly [Evidence
                     IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HIR3339209.1"
                     /translation="MNADPKGLQINVMGREFRVACPEDEQKGLLEAVDYLNKKMNEIR
                     DNGKVIGLERIAIMAALNIAHELLATKVGGFDIAQLKRRMNGMATMLDRAMNDQSKLF
                     "
     gene            complement(1018..1218)
                     /locus_tag="AC158Q_05240"
     CDS             complement(1018..1218)
                     /locus_tag="AC158Q_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339210.1"
                     /translation="MEADLKALEQKLGQFVELCQRLRVDNQQLRQQLASAMSDNKQLS
                     EKIGTATHRLESLLNQIPEEDA"
     regulatory      1520..1791
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1891..3879
                     /locus_tag="AC158Q_05245"
     CDS             1891..3879
                     /locus_tag="AC158Q_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3339211.1"
                     /translation="MSFRKGSAAVLSATACAVFAAAVLAAEAPLTYVPPVIVTATRFG
                     EPGERFPIGVEVITREDIRRSTAATVPDLLRQLPGVRTRDNSSSPNVQVDLRGFGIFG
                     DQNTLVLLDGVRVSENEQATVNWAGIPLSAIERIEILRGSGAVLYGGGATGGTINIIT
                     RTPAAGERSASIYGGAASYGTGEAGIGANLAGDGVGLRFNASRLETDNYRDNNALRLG
                     NALADIRWTGSGGTLSLKAGADNQSLRLPGALNEAQIAANRRQAATPRDFSERWGGFF
                     NVGGEARLGAAEVAANLGYREKNIHASIIGSPIDSDTRAWSFSPRLKLPHRLGGWQHS
                     FVAGLDWDDWQFDNSTAVFGAPRRPSAFQRNGAFYAQNTTALGSDWSLALGARRQHVE
                     YGVTDQANPAQTDARRRNLSAWEAAARYRASAVVAAYGKFGASFRAPNVNDVYNLFTG
                     AVSLLEPQTSHDREIGLDIRSGPASYRVALYHMDINNELFFDPQAFVNRNLPPTRRYG
                     AELDGKWRTAGALDFFANYTYAVSQCRAGDFGGVALAGNDVPLVPRHAFNIGAGWAFA
                     PRARADAVLRYVGKQTFDSDEADTYGRKMPAYTVVDLKLAWDNRGWQLGAGVKNLFNE
                     KYFSYGVFSPAFPPFPADFFAYPAPERSLFVSAQYDFR"
     gene            3952..5706
                     /gene="msbA"
                     /locus_tag="AC158Q_05250"
     CDS             3952..5706
                     /gene="msbA"
                     /locus_tag="AC158Q_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005319 - lipid carrier activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HIR3339212.1"
                     /translation="MTNSALYLRLLSHVRPYWRVFALGILGMMVVAATEPALPALMKP
                     LIEGTFIDKDPVLMRWMPVLIVALFAVRGLAAYLAAYALNWVGTRLVTDLRNAMFARL
                     LALPATFYERQPTGVLISKLTFDVTQVTAAATSALTIVFRDTLAIVGLLAFLLWLNWK
                     LTLFALAMMPLIVAVVRVLSVRLRVSSREVQNAMGQITQVIDEAIGGHKVVKLFGGQA
                     HEARRFDEEANRVRRHQMKQAMASAASAPIVQLIAAGALAAIIWYAMLQSSAAQLSVG
                     EFAAFVTGMLMLTAPLKRVTDINEYLQKGLAAAESVFRLIDEAPEPDRGTEVMGRARG
                     ELRFEDVSFSYQDAGKPALESVNLAIAPGETVALVGASGAGKTTLANLVPRFYHPARG
                     RVLLDGHDLEALTLASLRANIALVSQDVVLFNDTVAANIAYGTMNGAAEKDIVAAAEA
                     AHAMEFIREMPQGLATLVGENGVKLSGGQRQRLAIARALLKNAPVLVLDEATSALDTE
                     SERHVQAALEALMRGRTTLVIAHRLSTVEKADRIVALDRGRIAETGTHRELLARGGIY
                     DKLYRIQFASAEPANGGR"
BASE COUNT          912 a         1882 c         1901 g         1015 t
ORIGIN      
        1 caggcgcccg atgcattccc actcggcctg cgtcaccggg gtgatggaca ggcggttgcc
       61 gcgctgcagg actggcaggt tcttcagcgc tttctggccg cgcagctcgg cgaggctcac
      121 gacgcgcgtc ttcttcacga atttcacgtc cacgttgaac cagcgcggat tgtccttcga
      181 cgcgccggga tcgtattgcc gggatttccg gtcgaactgc gtctcgtcgg ggtaggcggc
      241 cccggcaacc tcgaggatgc cggcgatgcc gggcacctcg cagctcgaat ggtagaagaa
      301 cccgcggtcg cctttccgca tcgcatcgcg catgaaatta cgcgcctgat agttgcgcac
      361 gccgctccac gcggtgacct tgtccctcgc gagatggtcg atgccgtagg tggagggctc
      421 ggatttgaag agccagtagc tcatacgggt gtcgggtgtc gagggtcggg tgatgcgtgg
      481 cgagtgacca gggcgtggca aaaaaaatgc ggctcgcgcc gcatttctat ttagatcccc
      541 cgcctgtgcc gtcaggccag catcttgaac caacggttca aggttggtcg ccttccgagc
      601 ccatcaggcg cttccatgtc ggacgcgcac accggcgctg cttgagtcgg cgacctcagt
      661 taactatgtt ggtttagaaa cttaagcctt ttacgaacac cgcaggggaa gctcagaaca
      721 atttgctctg atcgttcatc gcccgatcga gcattgtcgc catgccgttc attctacgct
      781 tcagttgcgc gatgtcaaag ccgccgacct tggtggcgag aagctcgtgc gcgatgttga
      841 gcgcggccat gatggcgatg cgctcgagcc cgatgacttt cccgttgtcg cggatttcat
      901 tcatcttctt gttgaggtaa tccacggcct cgagcaggcc cttctgctcg tcttccgggc
      961 acgcgacgcg aaattcgcgt cccatgacgt tgatttgcaa tcccttcgga tccgcgttca
     1021 tgcgtcttct tcgggaatct ggttgagcag actctccagg cgatgggtcg cggtgccgat
     1081 tttttccgag agctgcttgt tatcgctcat cgcggaagcg agttgctggc gcaattgctg
     1141 gttgtcgacg cgcagccgct ggcacagttc gacaaactgg cccagtttct gctcgagcgc
     1201 cttcagatcc gcttccatcc gccacactat agtttcaaaa atcctgttag gtcaactgct
     1261 aacgttgatt tttgaatgtt atttggcatc aatgtcatga gtcatcccgg cgcgctattc
     1321 atgcgctccg gagccgtgcc ggaccgcatt cgggccatcc gcaatggcgt cgcttaagcg
     1381 gctgacacgg cgcggaaaac cctgcgttgc cggcggcggg cacggtggtt gcggcgccgc
     1441 cgcatccggg ttgagccggc cggtgccggc gcctacaatc cgtgccgtgc gggcggagcg
     1501 ctatccgtga gcaacagcca gcccgcactc gataccgggt gcccgtccgc gtttcccgcg
     1561 gcgggttaaa cgggaaacag gaacggtagt tacaaggtac aagttgcaag tttcaagggt
     1621 ttttccttgt ttcttggaac ttgaagcttg catctgtcgt tgaagcctgt gctgcccccg
     1681 caacggtaag cgagcgtgga tccgtcacta cgccactgag cagggatgct tgggaaggcg
     1741 gcggatcaag ttctcgcgag cccgtagacc ggcccggatc gatggtggaa atgccgcggg
     1801 gaagcggcgt gcctcttcgt ccggcaccgg ctctcccgcg tcgtttcctt gcgcctgatt
     1861 gcatgcggcg ggcatgcgcg aggagaaaac gtgtcgttca ggaagggatc cgccgccgtg
     1921 ctgtcggcca cggcgtgcgc ggtattcgcc gcggccgtat tggcggccga agcgccgctc
     1981 acctacgtcc cgccggtcat cgtcaccgcc acgcgcttcg gcgaacccgg cgagcgcttc
     2041 ccgatcggcg tcgaggtcat cacccgggaa gacatccggc gcagcacggc ggccaccgtg
     2101 cccgacctgc tgcgccagct cccgggcgtg cgcacgcgcg acaactcgag ttcgcccaac
     2161 gtgcaggtcg acctgcgcgg tttcggcatt ttcggcgacc agaacaccct ggtgctgctc
     2221 gacggcgttc gcgtgagcga gaacgagcag gccacggtca actgggcggg cataccgctg
     2281 tcggcgatcg agcgcatcga gatcctgcgc ggctcgggcg cggtgctcta cggcggcggg
     2341 gccaccggcg gcaccatcaa catcatcacc cggacgcccg cggccggcga gcgttccgca
     2401 tcgatttacg gcggcgccgc cagctacggc acgggtgaag ccggcatcgg cgccaatctc
     2461 gccggcgacg gcgtcgggct gagattcaac gcgagccgtc tcgaaaccga taactaccgc
     2521 gacaacaacg cgctgcgcct gggcaacgcg cttgccgata tccgctggac gggaagcggc
     2581 ggaacgctgt cgctcaaggc cggcgccgac aaccagtcgc tgaggctgcc gggcgcgctg
     2641 aacgaggcgc agattgccgc caaccgcagg caggccgcga caccccggga cttcagcgag
     2701 cgctggggcg gatttttcaa cgtgggcgga gaggccaggc tcggcgcggc cgaggtcgcg
     2761 gccaacctgg gataccgcga gaaaaacatc cacgccagca tcatcggcag cccgatcgac
     2821 tccgataccc gcgcgtggtc gttctcgccg cggctcaagc ttccccaccg cctgggcggc
     2881 tggcagcaca gcttcgtcgc cgggctggac tgggatgact ggcagttcga caacagcacc
     2941 gccgtattcg gggcgccccg gcgccccagc gcgttccagc gcaacggcgc cttctatgcc
     3001 cagaatacga cggcgttggg cagcgactgg agcctggcgc tgggcgcgcg ccgccagcat
     3061 gtggagtacg gcgtcaccga ccaggcgaac ccggcgcaaa cggacgcgcg caggcgcaat
     3121 ctttccgcct gggaggccgc cgcgcgttat cgcgcgagcg ccgtggtggc ggcttacggc
     3181 aagttcggcg ccagcttccg cgcgcccaac gtcaacgacg tctacaacct gtttaccggc
     3241 gcggtgagcc tgctcgagcc gcagacctcg catgaccgcg agatcgggct cgatatccgc
     3301 tccgggcccg cgagctaccg cgtcgcgctg taccatatgg atataaacaa cgagctcttc
     3361 ttcgacccgc aggctttcgt caaccgcaac ctgcccccga cgcgtcgcta cggcgcggag
     3421 ctcgacggca aatggcgcac cgccggcgcg ctcgattttt tcgcgaatta cacgtacgcg
     3481 gtgtcgcaat gtcgcgccgg agacttcggc ggcgttgcgc tcgccggcaa cgacgtgccg
     3541 ctcgtgccgc gccatgcctt caatatcggc gccggctggg cgttcgcgcc gcgcgcccgc
     3601 gcggacgccg tgctgcgtta cgtgggcaag cagacgttcg actccgacga ggccgacacc
     3661 tacggccgca agatgccggc gtacaccgtg gtcgatctga agctcgcctg ggacaaccgg
     3721 ggctggcagc tcggcgccgg cgtgaagaac ctgttcaacg agaagtattt cagctacggc
     3781 gtgttcagcc cggccttccc gccgtttccc gccgatttct tcgcctaccc ggcgccggag
     3841 cgctcgctgt tcgtgtccgc gcagtacgat ttccggtagg cgttttcgag gggaaacggc
     3901 gcggctgcgg cgtttctctt ttggccccgg gtttgttatc gttccgcggt catgaccaac
     3961 agcgcgctct atttgcggct gctttcgcac gtccggccgt actggcgcgt gttcgcgctc
     4021 ggcatcctcg gcatgatggt ggtcgcggcg accgagcccg cgctaccggc gctgatgaag
     4081 ccgctgatcg agggcacgtt catcgacaag gatcccgtcc tcatgcgctg gatgccggtg
     4141 ttgatcgtcg cgctgttcgc agtgcgcggg cttgccgcgt acctcgccgc ctacgcgttg
     4201 aactgggtgg ggacgcgcct ggtgaccgat ctccgcaacg cgatgttcgc gcgcctgctc
     4261 gcgctgcccg ccaccttcta cgagcggcag cccaccggcg tgctcatctc caagctcacg
     4321 ttcgacgtga cccaggtcac cgccgccgcc accagcgcgc tcaccatcgt gttccgcgac
     4381 acgcttgcca tcgtcgggct gctggcgttc ctgctgtggc tcaactggaa gctcacgctg
     4441 ttcgcgctgg cgatgatgcc gctgatcgtg gccgtggtgc gggtgctgag cgtgcggctg
     4501 cgcgtgtcaa gccgcgaagt gcagaacgcg atggggcaga tcacccaggt gatcgacgag
     4561 gcgatcggcg ggcacaaggt ggtgaagctg ttcggcggcc aggcgcacga ggcgcgccgc
     4621 ttcgatgagg aggcgaaccg cgtgcggcgt caccagatga agcaggccat ggcgtcggcg
     4681 gcgagcgcgc ccatcgtgca gctgatcgcg gcgggcgcgc tggcggccat catctggtac
     4741 gcgatgctgc aatcgagcgc ggcgcagctc tcggtcggcg agttcgcggc tttcgtcacg
     4801 ggcatgctga tgctgacggc gccgctgaag cgcgtgaccg acatcaacga gtacctgcag
     4861 aaggggctcg ctgccgccga gagcgtgttc cggctgatcg acgaggcgcc cgagcccgac
     4921 cgcggaacgg aggtgatggg ccgcgcgcgc ggcgagctcc ggttcgagga cgtgagcttt
     4981 tcctaccagg atgccggcaa gccggcgctg gaatccgtca acctcgccat cgcgccgggc
     5041 gagacggtcg cgctggtcgg cgcctcgggc gccggcaaga ccacgctcgc gaacctggtg
     5101 ccgcgcttct accacccggc gcgcgggcgc gtcctgctcg acgggcacga cctggaggcg
     5161 ttgacgctcg cgagcctgcg ggcgaacatc gcgctggtga gccaggacgt ggtgctgttc
     5221 aacgacacgg ttgcggccaa catcgcctac ggaacgatga acggcgccgc cgagaaggac
     5281 attgtcgccg cggccgaggc cgcgcacgcc atggagttca tccgcgaaat gccgcagggg
     5341 ctcgcgacgc tggtgggcga gaacggggtg aagctctcgg gcggccagcg ccagcggctc
     5401 gcgatcgcgc gggcgctgct gaagaatgcg ccggtgctgg tgcttgacga ggcgacctcc
     5461 gcgctcgaca ccgaatcgga gcgccacgtg caggccgcgc tggaggcgct gatgcgcggc
     5521 cgcaccaccc tcgtcatcgc gcaccggctg tccacggtgg aaaaagccga ccgcatcgtg
     5581 gcgctcgacc gcggtcgcat cgccgagacc ggcacccacc gcgagctgct cgcccggggc
     5641 ggcatctacg acaagctgta ccgcatccag ttcgccagtg cggagccggc gaacggcggg
     5701 cgctaggcac
//
LOCUS       DBNGSG010000199        11613 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1932782, whole genome shotgun sequence.
ACCESSION   DBNGSG010000199 DBNGSG010000000
VERSION     DBNGSG010000199.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 11613)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11613)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11613
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1932782"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="AC158Q_05255"
     CDS             <1..548
                     /locus_tag="AC158Q_05255"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ANTAR domain-containing response regulator"
                     /protein_id="HIR3339213.1"
                     /translation="RSAGYRVTGTASSTDALAIAGRDSPDLALLDVRMPGMDGIELGS
                     QLRGQGIPFLFLSAYGDQEIVSQAAEGGALGYLVKPLDIQQIVPSIEAALKRAQEIGN
                     LRRSEKQLTTALGGSREISMAVGMLMMRDRLNREQAFDLLRSNARSQRRPVAEVASEL
                     LASAEKLYTIKHLKNTGRRRP"
     gene            993..2849
                     /locus_tag="AC158Q_05260"
     CDS             993..2849
                     /locus_tag="AC158Q_05260"
                     /inference="COORDINATES: protein motif:HMM:NF053590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOL_0244 family TonB-dependent receptor"
                     /protein_id="HIR3339214.1"
                     /translation="MLTALAIFPAFAADHLAASLADFSLEQLANIEVTSVSKQAERLT
                     DAPASIFVITNDDIRRAGVRTLPEALRLAPNLQVARVNATKYAISARGFNNAIGNKLL
                     VLIDGRTVYTPLFSGVFWDQQDVMLEDVERIEVISGPGATLWGANAVNGVINVITRAA
                     KDTQGALVSLGLGNRDEGAAFRYGGKLGESGHYRVYGKSAELQNTKRANGTSVTDGWQ
                     MGQIGFRADWRHAGDGFTLQGDTYSGKSEFLITPTVVVPPITVSGTNLLARWTRQLSD
                     GSDIRLQAYVDHTDRDDPLFFRPRADIFDIDFQHGIPLGAHKVLWGGGYRHARDDVGA
                     GIAFSRFIPENSRLEWMNLFVQDEIRLTAKLDFTAGIKLERNDYTGWEFLPSLRFAWK
                     PQDQRLVWGAVSRAVRAPSRFDRDVFFPPAGPFVIRGGPNFVSEVANVFEVGYRAQPS
                     RVLTYSITAFRHDWERLRSATSQPAPIFFVNENEGFVNGVEAWATFQPTRSWRLSGGF
                     TTLHEKLSPRPGNNETLANDPDHQWMLRSMFNLTERHEFDVMIRRTGSLPFQRVPAYT
                     AVDARWGWHVGRNTEVSLTLQNLFDREHAEFGAAPGRSEFGRSLFLKLLWRI"
     gene            2861..3457
                     /locus_tag="AC158Q_05265"
     CDS             2861..3457
                     /locus_tag="AC158Q_05265"
                     /inference="COORDINATES: protein motif:HMM:NF053591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOL_0243 family YfiR-like periplasmic protein"
                     /protein_id="HIR3339215.1"
                     /translation="MPASRMRIETGSRFLRMLGRMVLFACVFGLQAWHANAADDAATL
                     EHRIKAAYLYQFAGYVEWPPDSFAQPDTPVTIAVLGAESLAAELNQVVTGRTVGGRTV
                     VVKRVKAGEPLAGVHILFIGRAERARLVPLAQASQSRAILTVTESDGALAQGSMINFV
                     LVDRRVRFEVALDPVEKSGLKLSSRLLAVAQQVRTEAP"
     gene            3457..4986
                     /locus_tag="AC158Q_05270"
     CDS             3457..4986
                     /locus_tag="AC158Q_05270"
                     /inference="COORDINATES: protein motif:HMM:NF053592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase, YfiR-like
                     protein-associated"
                     /protein_id="HIR3339216.1"
                     /translation="MLRVIARSIRHKLALIVLATTLVALAVTGVALVIYDLRAYREAG
                     FSDLVTQAEILGRASTAALAFDDVKAANEDLLLLKAKPGIASAAIYNARGGLFASYTR
                     RELDTGFPRLPESEGMRIEGDHFVLFKRIVENNEILGTVYIKADYEAMARLKDYLGIF
                     GAVSALSLIVALLVYVWLQNVLTRPILNVAEVTRRVVETRDFSLRARKTTQDEVGELA
                     DALNDMLAEIGRRSEALEQSNRFLEHEMAERQHADAELRKLNEELEQRVADRTRELTA
                     ANKEMEGFSYSVSHDLRTPLRAIDGYSRILQEDYAGKLDDEGRRLLGVIRDNSQKMGQ
                     LIDDLLAFARLGRKALAISEIDMKRMAEETLGEVTSGGQAPALMLGMLPPAHGDPSLV
                     KQVWANLLANAVKFSSKREQPRLEVGGYSDGEHNVYCVKDNGAGFDMRYYAKLFGVFQ
                     RLHRFEEFSGTGVGLAIVQRVVVRHGGRVWAEGKVGEGAAFYFSLPKGRQPEETQNEQ
                     L"
     gene            4973..5416
                     /locus_tag="AC158Q_05275"
     CDS             4973..5416
                     /locus_tag="AC158Q_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3339217.1"
                     /translation="MSSFETVEILLVEDNPTDAELTMRALRKGNLANHITWVKDGEEA
                     LDFIFRTGGYAERPDANPKLILLDIKLPKVDGIEVLKRIKSDDRTRIIPVVMVTSSAE
                     GRDIAESYKLGVNSYVVKPVDFEQFSDTLSKAGFYWMLMNRVPET"
     gene            5438..8071
                     /locus_tag="AC158Q_05280"
     CDS             5438..8071
                     /locus_tag="AC158Q_05280"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HIR3339218.1"
                     /translation="MIERVMKKELRILLVEDQPTDADLEIRELRRAGLSVSHQLVETE
                     PAFRESLREFRPELIISDFSMPHFDGMSALAITREFAPDVPFIFVSGTIGEEYAIRAL
                     KNGATDYVLKSNLVRLPAAVERALQDSKERAARRKAEEEIRYQRSFLRQVIDLDRNMI
                     FTKDIQGRFVLVNLAVAEMFGTSVEAMLGKTLAEFGLSKEMTERMRVDDMEVMETGGE
                     KFIAEDRVVDAAGRERWFQLVKRPLIGPDGKASMVLGVATDITERKSQELKIARLSRI
                     HAVLSGINSAIVRIRDRQQLYEEACRIAVEHGGFGIAWIGMLDPDTLVITPVASAGVE
                     TESFLAKSQSTAHPGTSLGRGMVGIAVREKRAAFSNDLTKEDFSSGGERRKEALRRGY
                     RSVIVLPLLVEGAAVGNLSLFAKEPDFFDDEEIKLLTELAGDISFALEHIAKSERLEH
                     LVFYDGLTGLANRKLFDERIEHFIATSEARRSGLAVVVVDVERMRNINESLGIAAGDE
                     LLRQFARRLEASVEESGSVARVSGDHFGVKIRGAGDAANVAHFVESRVINGLNEPYSI
                     AGQELRVPLKAGIAVYPGDGENAAALYTNAEAALRKAKEKGERFLFYASEMNARVAEH
                     LKLENDLRNALLKEQYVLHYQPKFDLASGQMAGMEALIRLKHPVRGLVSPAEFIPLLE
                     ETGMILEVGRWALRRAALEHGAWRAQGYRPPRVAVNVSLVQLRRRDFVEDVKDALAGM
                     SGAGECVDIEITESMLMEDIEGSIDKLNALRGAGLQIALDDFGTGYSSLSYLARLPIN
                     SLKIDRSFIVQMPKSPEQMAIVSAVISLARALNLKVVAEGVETHEQANLLRLLRCDEV
                     QGYLFGRPMPPEELEKLLPRD"
     gene            complement(8111..8347)
                     /locus_tag="AC158Q_05285"
     CDS             complement(8111..8347)
                     /locus_tag="AC158Q_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339219.1"
                     /translation="MQGWYELRVTGSAGTSAGISAGVDEGIAVGENGRLCSLSSGGAR
                     RFESQQQALEYLGKIKVSGDYQFEAVLCGIRAAA"
     gene            complement(8391..8759)
                     /locus_tag="AC158Q_05290"
     CDS             complement(8391..8759)
                     /locus_tag="AC158Q_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HIR3339220.1"
                     /translation="MKLHLMPNAGLQLFTGYGAGYVAVNNARHENCVLVTPQEVVEWK
                     VSAFDALTAADFGFIADLKPEIVVLGTGATQRFPRAELARALAAAGAGVEIMDTRAAC
                     RTYNILASEGRKVVAAILLD"
     gene            8831..10015
                     /gene="alaC"
                     /locus_tag="AC158Q_05295"
     CDS             8831..10015
                     /gene="alaC"
                     /locus_tag="AC158Q_05295"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HIR3339221.1"
                     /translation="MEEFARIKRLPPYVFNVTGELKLAARRRGEDVIDFSMGNPDQPT
                     PRHIVEKLVETVQRPNTHGYSVSKGIYRLRRAICGWYKSRYDVELDPDSEAIVTIGSK
                     EGIAHLALATLDQGDTVLVPNPSYPIHIYGPVISGADIRHVPMAPDVDFFEEVERAFK
                     DTYPKPKMLIVNFPSNPTTQCVDLAFFERLVALAREHHVYIVHDLAYADIVFDGYKAP
                     SIMQVPGARDIAVEFFTMSKSYNMAGWRVGYMVGNRELVTALARMKSYHDYGTFTPIQ
                     VASIIALEGPQDCVEVVRKVYQGRRDVMCAGLKAIGWNVEVPKASMYIWAPIPDQYRK
                     MGSLEFSKKLLADAKIAVAPGIGFGHYGDGHVRFALIENEARSRQALRGIKDMFKKDG
                     LL"
     gene            10012..11364
                     /locus_tag="AC158Q_05300"
     CDS             10012..11364
                     /locus_tag="AC158Q_05300"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HIR3339222.1"
                     /translation="MKAQGTRHKAKVSSRRSINVGLLGIGTVGSGTFAVLARNQEEIA
                     RRAGCAIRMKVVADKDLDRARRLVGNKAAVTADAGDVVSSPDIDIVVELIGGTGIAKE
                     LILKAIACGKHVVTANKALLAQHGNEIFAAAQKQDVMVAFEAAVGGGIPIIKALREGL
                     SANRIEWIAGIINGTSNFILSEMRDKGSSFPDVLKEAQQRGYAETDPTFDIEGVDAAH
                     KLTIMAAIGFGIPMQFKAVYTEGISKLTREDIRYADELGYRIKLLGIAKRKAKGIELR
                     VHPTLIPARRLIANVEGVMNAILVKGDAVGQTMYYGAGAGSEPTASSVVADLVDVARM
                     LTSDPEHRVPHLAFQPDQLSDIPILPMGEVETAYYLRLRVQDKPGVLAEITRIFAELS
                     ISIDAMVQKEPHEGEEQADIILLTHLTFEKHVNAAIAKIEKLPVVVGRITRIRLEELN
                     "
     gene            11384..>11613
                     /locus_tag="AC158Q_05305"
     CDS             11384..>11613
                     /locus_tag="AC158Q_05305"
                     /inference="COORDINATES: protein motif:HMM:NF012512.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR3339223.1"
                     /translation="MSHYTGLIEKYRDRLPVAKDARVISLGEGATRLIELKNLPTLVR
                     KKVRILAKFEGLNPTGSFKDRGMTMAVTRAVE"
BASE COUNT         2246 a         3477 c         3838 g         2052 t
ORIGIN      
        1 tgcggtccgc gggctatcgc gtcaccggaa cggcgagcag cacggatgca ctggcgatcg
       61 ccgggcgtga ttcccccgac cttgccttgc tcgacgtgcg gatgcccggc atggacggca
      121 tcgagctggg aagccagttg cgcgggcagg gcatcccttt tctgttcctg tcggcctacg
      181 gcgatcagga aatcgtgagc caggctgccg agggaggcgc gctcggctat ctcgtcaagc
      241 cgctcgacat ccagcagatc gtgccctcga tcgaagccgc actgaaacgg gcgcaggaaa
      301 tagggaatct tcggcgaagc gagaagcagc tcaccacggc gctgggcggt tcccgggaaa
      361 tcagcatggc ggtgggcatg ttgatgatgc gagaccgcct gaatcgcgag caggcatttg
      421 atctgttgcg gagcaacgcg cgatcgcagc gccgcccggt ggcggaggtg gccagcgagt
      481 tgctggcctc cgcagagaag ctttatacaa tcaagcactt gaaaaacaca ggccggcgca
      541 ggccgtagcc ttcaccatgg cattttgcag gctgtggagg gtgatttcta ggcaactgtt
      601 gttcacagtt tgaagcgtga gtccatattt tcgttagcgt gagttctttg cttgcaagct
      661 attgaatttg tgtttataag cgatgctaaa taaggcagcc cagtacccgc tgcccgcttc
      721 gcaggaactc agttggtgcg tgccgcccgg accctgcggt atggccattt caggctcagc
      781 ccccgcctgc agttgatgcc gctcgacttt atgtcgggga ggggagtgtg ggtgcgtcaa
      841 caaaaatgaa tgcagggcgc gtaaggctcg gcttccttgc gctcccactg cggccggttt
      901 cgcatgagaa tgccggcagc gaagcctgtc gtcctgcgcc gattcagccg cgtcaccggc
      961 aacaggggcg ggcacgatcc cgtcttgccg ggatgttgac ggcccttgcg atatttcccg
     1021 ctttcgccgc cgaccacctg gcggccagtc ttgccgattt ttccctcgag caactggcca
     1081 acatcgaagt tacatcggta tccaagcagg cggagcgttt gaccgacgcg cctgcgtcca
     1141 tcttcgttat caccaacgac gacatccggc gcgccggcgt aaggaccttg ccggaggccc
     1201 tgcgcctggc gcccaacctg caggtcgcgc gcgtgaacgc gaccaaatac gcgatcagcg
     1261 cgcgcggctt caacaacgcc atcggcaaca agctgctggt gctgatcgac gggcgcacgg
     1321 tgtatacgcc gctgttctcc ggcgtgttct gggaccagca ggatgtgatg ctcgaggacg
     1381 tcgagcgcat cgaggtgatc agcgggccgg gcgccacgct ctggggagcc aatgcggtga
     1441 acggcgtcat caacgtcatc acgcgcgccg caaaggacac gcagggcgcg ctcgtttcgc
     1501 tgggactcgg caatcgcgac gagggcgccg cgttccgtta cggtggaaaa ctcggcgaga
     1561 gcgggcatta tcgcgtctac ggaaagagtg ccgagctgca gaacacgaaa cgcgccaatg
     1621 gcacgtcggt gacggacggg tggcagatgg gccagatcgg attccgcgcg gactggcgcc
     1681 acgcgggcga cggtttcacg ctgcaggggg atacttacag cgggaaatcg gaattcctga
     1741 tcactccaac tgtcgtggtt ccgcccataa cggtttccgg cacgaacctg ctcgcgcgct
     1801 ggacccggca actgagcgac ggctcggaca tccggttgca ggcctatgtg gaccacaccg
     1861 accgtgacga tcctctcttc ttccggccca gggcggatat cttcgatatc gattttcagc
     1921 acgggattcc attgggggcg cacaaggtgc tgtggggcgg gggatatcgt cacgctcgcg
     1981 acgatgtcgg tgccggcatc gccttttcca ggttcatacc ggagaacagc cggctggagt
     2041 ggatgaattt gttcgtgcag gacgagatca ggctcaccgc gaagctggat ttcacggccg
     2101 gcatcaagct cgagcgcaac gactataccg ggtgggagtt cctgcccagc ctgcggtttg
     2161 cctggaagcc gcaggaccag cggctggtgt ggggcgccgt ttcgcgcgcg gtacgcgccc
     2221 cctctcgttt cgatcgagac gtatttttcc cgcccgccgg gccgttcgtc attcgcggcg
     2281 ggccgaactt cgtgtccgag gtcgccaacg tgtttgaagt cggttatcgc gcgcagcctt
     2341 cccgcgtcct gacctactcg attacggcat tccggcacga ttgggagagg ctgcgcagcg
     2401 ccaccagcca gccggctccg atctttttcg tgaacgagaa cgaaggtttc gtgaatggcg
     2461 ttgaagcatg ggccacgttc cagccgacgc gcagctggcg gctgagcggc ggcttcacga
     2521 cgttgcatga aaaattgagc cccaggccgg ggaacaatga aacgctggct aacgatcccg
     2581 accaccagtg gatgttgcgt tcgatgttca acctgacgga gcgacacgag ttcgacgtca
     2641 tgatacggcg caccggctcg ttgccgtttc agagggtccc tgcctatacg gcggtggatg
     2701 cgcgctgggg ctggcacgtc ggccggaata ccgaggtctc cctgaccctc cagaatcttt
     2761 tcgaccggga acatgcggag ttcggcgccg cgccgggacg cagcgaattc gggcgtagct
     2821 tgttcctgaa attgctttgg cggatctgac cggagaagcc atgccggcat ccaggatgcg
     2881 gatcgagacc ggatcgaggt tcctgcgcat gttggggcgt atggtgttgt tcgcatgcgt
     2941 cttcggtttg caagcatggc atgcaaatgc ggcggacgat gccgcaacac tggaacaccg
     3001 tatcaaggcc gcgtaccttt accagttcgc cggatacgtg gaatggccgc cggattcgtt
     3061 tgcgcagccc gacacgccgg tcaccatcgc cgtgcttggc gccgaatcgc tcgccgcgga
     3121 actgaaccag gtggtgaccg gccgcacggt aggcggccgg acggtggtgg tgaagcgtgt
     3181 caaggcgggt gagccgctgg cgggcgtgca catcctgttc atcggcaggg ccgagcgcgc
     3241 gcgcctcgtc cccttggcgc aggcctcgca gtcgcgcgcg atcctgaccg tgaccgaatc
     3301 ggatggcgca ctggcgcagg gcagcatgat caatttcgtc ctggtcgacc gccgggtgcg
     3361 gttcgaagtc gcgctcgacc cagtggagaa aagcgggctc aaactgagct cgcgcctgct
     3421 cgcggtcgcg cagcaagtgc gcacggaggc accctgatgc tgcgcgtcat cgcgcgctcg
     3481 atccgccaca agctggcgct gatcgtgctg gcgaccacgc tcgtggcgct ggccgtgacc
     3541 ggcgtggcgc tggtgatcta cgacctgcgc gcttaccgtg aagcgggttt tagtgatctc
     3601 gtcacgcagg cggagattct cggccgcgcc agcacggctg cgctggcgtt cgacgatgtc
     3661 aaggctgcca atgaggacct cctgctgctc aaagccaagc ccggaattgc cagtgccgcg
     3721 atctacaacg ccaggggcgg gctgttcgcg agctacacgc gccgcgagct ggacacgggt
     3781 ttccctcgcc tgcccgagtc ggaaggaatg cgaatcgagg gagaccattt cgtgttgttc
     3841 aagcggatcg tggagaacaa cgagattctc ggcacggtct atatcaaggc cgactacgag
     3901 gcgatggcgc ggttgaagga ttatctcggc atcttcggcg cggtctcggc gttgagcctg
     3961 atcgtggcgc tgctggtata cgtgtggctg cagaacgtgc tgacccggcc cattctgaac
     4021 gtggcggaag tgacgcggcg ggtcgtggaa acccgggatt tttcgctgcg cgctcgaaag
     4081 acgactcagg acgaggtcgg cgagctggcg gacgccttga acgacatgct ggccgagatc
     4141 ggacggcgct cggaagctct ggagcaatca aaccggttcc tggagcacga aatggcggag
     4201 cggcagcacg cggacgcgga gctgcgcaaa ctcaacgagg agctcgagca gcgggtcgcc
     4261 gaccgcaccc gggaacttac tgcggccaac aaggagatgg agggtttcag ctactccgtt
     4321 tcgcacgatc tgcgcacccc gctgcgcgca atcgacggct attcgcgcat cctccaggaa
     4381 gactatgccg gcaagctcga tgacgaaggc cgccgcctgc tcggcgtcat ccgggacaac
     4441 agccagaaga tggggcagct gatcgacgat ctcctggctt ttgcgcgcct gggccgcaag
     4501 gcgctcgcca tatccgaaat cgacatgaaa cgcatggctg aagagacgct cggggaagtc
     4561 acgtccggcg gccaggcgcc cgcgctgatg ctgggcatgc tgccgccggc gcacggggac
     4621 ccgtcgctgg tgaagcaggt atgggcgaat ctgctggcga acgcggtcaa gttcagcagc
     4681 aagcgcgaac agccccggct cgaggtgggc ggctacagcg acggcgagca caacgtctac
     4741 tgtgtgaagg acaacggcgc cggtttcgac atgcgctatt acgccaagct gttcggcgtg
     4801 ttccagcggc tgcaccggtt cgaggaattt tcgggcaccg gggtcggact cgccatcgtc
     4861 cagcgggtgg ttgtccgcca cggcgggcgc gtgtgggccg agggcaaggt cggagaagga
     4921 gcggcgtttt atttttccct gccgaaaggc aggcaacctg aggaaactca aaatgagcag
     4981 ctttgaaacc gtggagatcc tgctggtgga ggacaacccg accgacgcgg agctgacaat
     5041 gcgcgcgctg cgcaagggca accttgccaa ccatatcacc tgggtcaagg acggcgagga
     5101 agccctggat ttcatcttca gaaccggggg ctacgcggag cgtcccgacg cgaacccgaa
     5161 gctgatattg ctcgacatca agctgccgaa ggtcgacggc atcgaggtgt tgaagcgcat
     5221 caagtccgac gaccgcaccc gcatcatccc ggtggtcatg gtgacttcct ccgcggaagg
     5281 acgcgatatc gccgagagct acaagctcgg cgtcaacagc tacgtcgtca agccggtgga
     5341 tttcgagcaa ttcagcgaca ccctgtcgaa agcgggcttt tactggatgc tgatgaaccg
     5401 ggtgccggaa acttagtcgg gcctgaagcg gtaaaccatg atcgagcgag tcatgaagaa
     5461 ggaactccgt atactgctgg tcgaggacca accgaccgac gcggatctcg agatccggga
     5521 gttgcggcgt gccgggctga gtgtttcgca ccagctcgtc gagaccgaac cggcattccg
     5581 cgaatcgctg cgcgagtttc gtccggagct catcatttcg gatttttcca tgccgcattt
     5641 cgacggcatg tcggcgctcg cgattacgcg cgagtttgcg ccggacgtgc cgttcatctt
     5701 cgtctccggc accatcggcg aggagtacgc gatccgcgcg ttgaaaaatg gcgcgaccga
     5761 ttacgtgctg aagagcaacc tcgtccggct gcccgcggcg gtcgagcgcg ctttgcagga
     5821 cagcaaggag cgtgccgcgc ggcgcaaggc ggaggaggaa ataaggtatc agcggtcgtt
     5881 tctcaggcag gtcatcgacc tcgatcgcaa catgatattc accaaggaca tacaggggcg
     5941 cttcgtcctg gtcaacctgg cggtggccga aatgtttgga acctcggtcg aagccatgct
     6001 gggcaaaaca ctcgcggaat tcggcctcag caaggagatg acggagcgca tgcgcgtcga
     6061 tgatatggag gtcatggaga ccgggggcga gaagttcatc gccgaggaca gggtagtcga
     6121 tgcagcgggc cgggagcgct ggttccagct cgtcaagcgg ccgcttatcg gccccgatgg
     6181 caaggcgagc atggtgctcg gcgtcgccac cgacattacc gagcgcaaga gccaggagct
     6241 gaaaatcgcc cggctgagcc gcatccacgc ggtattgagc ggcatcaatt cggcgatcgt
     6301 gcgcatacgc gaccggcagc agctctatga ggaagcgtgc cgcatcgcgg tcgagcatgg
     6361 cggcttcggc atcgcctgga tcggcatgct ggacccggac accctggtga tcaccccggt
     6421 cgcgagcgcc ggcgtggaga cagaatcgtt cctcgcaaag agtcaaagca cggcgcatcc
     6481 cggcacttcg ctgggcaggg gcatggtcgg catcgccgtc agggagaagc gcgccgcgtt
     6541 cagcaacgat ctcaccaagg aagacttctc ctccggcggc gagcgcagga aagaggcgct
     6601 gcgccgcggc tatcgctcgg tcatcgtgtt gccgctcctg gtggagggcg cggcagtggg
     6661 gaatctatcg ctgttcgcga aggagcctga tttttttgat gacgaagaaa tcaagctgct
     6721 gaccgagctg gccggcgata tctcgttcgc gctggagcat atcgccaaga gcgagcggct
     6781 cgagcatctc gtgttctacg acggcctgac gggattggcg aaccgcaagc tgttcgatga
     6841 gcggatagag cacttcatcg ccacctcaga agccaggcga tccggtctgg ccgttgtcgt
     6901 ggtggacgtg gagcggatgc gaaacatcaa cgagagcctg gggatcgcag cgggagacga
     6961 actgctcagg caattcgcaa ggcgcctcga ggcaagcgtg gaagagagcg gcagtgtcgc
     7021 gcgcgtgagc ggggatcatt tcggcgtcaa aatccgcggt gcgggcgacg ctgcgaatgt
     7081 ggctcacttc gtcgagagcc gcgtgatcaa cgggctcaac gagccgtatt ccatcgccgg
     7141 acaggagctg cgggtgcccc tgaaggcggg catcgccgtc tacccgggcg acggggaaaa
     7201 tgccgcggcg ctgtatacca acgccgaggc ggcgttgcgc aaagccaagg aaaagggcga
     7261 gcgcttcctg ttctatgcat cggagatgaa tgcccgggtc gccgagcatc tgaaactgga
     7321 gaacgatctt cgcaacgcat tgctgaagga gcaatacgtc ctgcactacc agcccaaatt
     7381 cgatctcgcc agcgggcaga tggccggaat ggaggcgctg atacgcttga agcaccccgt
     7441 gcgcgggttg gtgtcacccg ccgagttcat tccgctgctg gaggaaaccg gcatgatcct
     7501 ggaagtcggg cgctgggcgc tcaggcgcgc ggcgctcgag catggcgcat ggcgtgccca
     7561 gggataccgt ccgccgcgcg ttgccgtcaa cgtttccctg gtgcagttgc ggcgcaggga
     7621 cttcgtggaa gatgtcaagg acgcgctggc gggaatgagc ggggccggcg agtgcgtcga
     7681 catcgaaatc accgagagca tgctcatgga ggacatcgag ggcagcatcg acaagctgaa
     7741 tgcgctgcgg ggcgcgggcc tgcagatcgc cctcgacgac ttcggcacgg gctactcgtc
     7801 gttgagctat ctcgcgcggc ttccgatcaa ttcactgaaa atcgaccgct cgttcatcgt
     7861 tcagatgcca aagagtcccg agcagatggc tatcgtctcc gccgtgattt cgctcgctcg
     7921 cgcgctgaac ctcaaggtgg tggcggaagg cgttgaaacc catgagcagg ccaacctgct
     7981 gcgcctgctg cgctgcgacg aagtccaggg ttacctgttc gggaggccga tgccgccgga
     8041 agaactggag aagctgttgc cccgggattg acgagcccgg atggaggcct ggagcaggtg
     8101 gatggaacct tcaggccgcg gccctgatgc cgcacaggac agcttcgaac tggtagtcgc
     8161 cggaaacctt gattttgccc aggtactcca gcgcctgttg ctgcgactcg aagcgcctgg
     8221 cgccacccga tgacagggag cagagtcggc cattttcacc gacggcgatg ccctcatcga
     8281 caccggcaga aatgcctgcg ctggtgccag cgctccccgt gacgcgcaat tcgtaccaac
     8341 cctgcatgct ggttcttccc cgaccgcttg cgcgcagtct aacagcacga tcagtccaat
     8401 agaatagccg ccaccacctt gcggccctcg gacgcgagga tgttgtaggt ccggcaggcg
     8461 gcgcgtgtat ccatgatttc cacgccggcg cccgcggcgg ccagcgcgcg ggccagctcg
     8521 gcgcgcggaa aacgctgggt tgcaccggta cccaatacca cgatttccgg tttcagatca
     8581 gcgataaaac cgaagtccgc cgcggtcaac gcatcaaagg cggatacttt ccattcgact
     8641 acttcctgag gcgtgaccag cacgcaattc tcatggcggg cattgttgac ggcgacgtag
     8701 ccggcgccgt acccggtaaa caattgcagg cccgcgttgg gcatcaggtg cagcttcata
     8761 acctgctgat ttgcttaagg ttagttgctc cgatagaata acacgctttt gcaccgcaat
     8821 aaccggcctc atggaagagt ttgcccgcat caagcgcctg cctccgtacg tctttaacgt
     8881 gactggagag ctgaagctcg ccgctcgccg gcgcggcgag gacgtgatcg acttcagcat
     8941 gggcaatccc gaccagccga ccccgcgcca tatcgtggag aagctggtcg agaccgtgca
     9001 gcgccccaac acccacggct actcggtgtc gaagggcatc taccggctgc ggcgcgcgat
     9061 ttgcggctgg tacaagtcgc gctacgacgt cgagctcgac ccggattccg aagcaatcgt
     9121 caccatcggt tcgaaggagg gcatcgcgca cctggcattg gcgacgctcg accagggcga
     9181 caccgtgctg gtgccgaacc cgagctaccc gatccacatt tacgggcccg tcatctccgg
     9241 tgctgacatc cggcatgtgc cgatggcgcc ggacgtggac ttcttcgagg aggtcgagcg
     9301 cgcgttcaag gacacttacc cgaagccgaa gatgctgatc gtcaactttc ccagcaaccc
     9361 gacgacgcag tgcgtagacc tcgcgttctt cgagcggctg gtggcgctgg cgcgggagca
     9421 ccacgtttac atcgtgcatg atctcgctta cgccgacatc gtgttcgacg gctacaaggc
     9481 gccctccatc atgcaggtgc cgggcgcacg cgacatcgcg gtcgagttct tcaccatgtc
     9541 gaagagctac aacatggcgg gctggcgcgt gggctacatg gtcggcaacc gcgaactggt
     9601 cacggcgctc gcgcgcatga agagctatca cgactacggc accttcacgc cgatccaggt
     9661 ggcttccatc atcgcgctgg agggtccgca ggactgcgtg gaagtcgtgc gcaaggtgta
     9721 ccagggccgc cgcgacgtca tgtgcgcggg gctgaaggcg atcggctgga acgtcgaggt
     9781 gccgaaggcg agcatgtaca tctgggcgcc gatcccggat cagtaccgca agatgggatc
     9841 actcgagttc tcgaaaaagc tgctggcgga cgcgaagatc gccgtggcgc cgggaatcgg
     9901 attcggccat tatggcgacg gtcatgtccg tttcgcgctc atcgagaacg aggcgcgcag
     9961 ccgccaggcg ttacgcggga tcaaggacat gttcaagaag gacgggctgc tgtgaaggca
    10021 caaggcacaa ggcacaaggc gaaagtgtcc tcgcgcaggt cgatcaacgt ggggctgctc
    10081 ggcatcggca cggtgggcag cggcacgttc gcggtgctcg cgcgcaacca ggaggagatc
    10141 gcgcgccgcg cgggctgcgc catccgcatg aaagtggtgg ccgacaagga tctggaccgg
    10201 gcgcgccgcc tggtcggaaa caaggccgcc gtgaccgcgg atgccggcga cgtcgtgagc
    10261 agccccgaca tcgatattgt cgtcgagttg atcggcggca ccggcatcgc gaaggaactg
    10321 atcctcaagg cgatcgcctg cggcaagcac gtcgtcacgg ccaacaaggc gctgctggcg
    10381 cagcacggca acgagatctt cgccgcggcg cagaagcagg acgtaatggt cgcgttcgag
    10441 gcggcggtcg ggggcggcat acccatcatc aaggcgttgc gcgaagggct ttccgcgaac
    10501 cgcatcgagt ggatcgccgg catcatcaac ggcaccagca atttcatcct gtcggagatg
    10561 cgtgacaagg gatcgagctt ccccgacgtg ctgaaggaag cgcagcagcg gggctacgcc
    10621 gaaaccgacc ccaccttcga catcgaaggc gtggacgcgg cgcacaagct caccatcatg
    10681 gcggctatcg gcttcggcat cccgatgcag tttaaggcgg tttacaccga gggcatctca
    10741 aagctgacgc gcgaggacat ccgctacgcg gacgagctgg ggtatcgcat caagctgttg
    10801 ggcattgcca agcgcaaggc aaaggggatc gagttgcggg tgcatccgac actgataccc
    10861 gcgcgccggc tgatcgccaa cgtggagggc gtgatgaacg cgatcctggt gaagggcgac
    10921 gcggtggggc agaccatgta ctacggcgcg ggcgccggat cggagccgac ggcctcctcg
    10981 gtggtggccg acctcgtcga cgtcgcgcgc atgctgactt ccgaccccga gcaccgcgtg
    11041 ccgcatctcg ccttccagcc ggaccagctt tccgatatcc cgatcctgcc gatgggcgag
    11101 gtcgagaccg cgtattacct gcgcctgcgc gtgcaggaca agccgggcgt gctcgccgaa
    11161 atcacgcgca ttttcgccga gctgtcgatc tccatcgacg cgatggtgca gaaggagccg
    11221 cacgagggcg aggagcaggc ggacatcatc ctgctcaccc acctcacgtt cgagaagcac
    11281 gtgaacgcgg caatcgcgaa gatcgagaag ctgccggtgg tggtcggcag gatcacccgc
    11341 atccggcttg aagaactgaa ttgaaccgcc aagaccaccg ggaatgtctc attacacagg
    11401 tttgatcgaa aaataccgcg accgcctgcc ggtcgcgaag gacgcgcgcg tgatctccct
    11461 cggcgagggc gcgacgcgtt tgatcgaatt gaagaacctg cccacgctcg tccgcaagaa
    11521 agtccgcatt cttgccaagt tcgagggcct gaatcccacc ggctcgttca aggaccgcgg
    11581 catgacgatg gcggtcacgc gtgcagtgga aga
//
LOCUS       DBNGSG010000200        13087 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3122263, whole genome shotgun sequence.
ACCESSION   DBNGSG010000200 DBNGSG010000000
VERSION     DBNGSG010000200.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 13087)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13087)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13087
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3122263"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..2624)
                     /gene="mutS"
                     /locus_tag="AC158Q_05310"
     CDS             complement(45..2624)
                     /gene="mutS"
                     /locus_tag="AC158Q_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HIR3339224.1"
                     /translation="MQQSLERHTPMMQQYLRIKAQHADKLLFYRMGDFYELFYDDAER
                     ASRLLDITLTRRGESAGEAIKMAGVPHHSVEQYLAKLVKLGESVAICEQIGDPGASKG
                     PVERQVTRVVTPGTLTDAALLDEKRDNLLLALCPQRGTVGLAWLSLASGRFTAMETAA
                     ANLAAELERLAPSEILVAEDTAADFLNGAAAAVKRLPLWQFDLDAARRALARQFETHD
                     LSGFGASDLTVAVSAAGALLEYARATQGTAIAHVKSLTVEHERAWVRMDPATRRNLEL
                     TETIRGEPAPTLYSLLDTCATGMGSRLLRQTLHHPVRDRAVLSARLDAVEALIARDGA
                     APYRGLHETLRQCVDVERITARIALKSARPRDLSGLRETLGLMPGLHATLAALVGSKL
                     AELAGALAPQGRLAALLAAAIKPEPAAVLREGGVIADGYDAELDELRAIQTNCGEFLV
                     ELETRERARTGIANLKVEYNRVHGFYIEVGRAQAGKVPDDYRRRQTLKNAERYITPEL
                     KAFEDKALSAQDRALAREKLLYDLLLDDLAPYLPRLQIAAGALAELDLLSAFAERAAA
                     LDYVRPDFADEPLIEIDAGRHPVVEQQVENFIANGARLADERRMLIVTGPNMGGKSTY
                     MRQVALIVLLAHCGSFVPAQRVRLGPLDAIYTRIGASDDLAGGRSTFMVEMTEAAFTL
                     HHATPQSLVLMDEIGRGTSTLDGLALAWAIARHLVEKTRSLTLFATHYFEMTRLAADY
                     RQAANVHLDAVEHKDSIVFLHNVEEGPASQSYGLEVAALAGVPGAVIRAARRRLAELE
                     EHGLAGGPQRDLFAAGAKNAQDAPASEEHAVVKRLRELDPDALTPKEALELLYQLRKQ
                     LDE"
     gene            2737..3942
                     /locus_tag="AC158Q_05315"
     CDS             2737..3942
                     /locus_tag="AC158Q_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339225.1"
                     /translation="MQSHLTTFRAVRLLAAAAAWTVFFTGQLAWFAGTAQGAAGPDPG
                     PQPLLDSTARLIAGMEPTHPDHAELTRSKEWQQHSGAIQSSWIRVRDGQLAAVTAWRQ
                     AELPPGCPVGQTLFYPFSGPDFLNAHWLFPGCENLVMFGLERIGVVPDVGSMSSQEFT
                     RLLTNTRSFMVNLFARNYFVTDTMQKDLRSAEQLRGVVPVFMVLMALSGCEVLRVAPL
                     ELERAPGARAPAKGRKRELRGVTIEYRVTGTSQVRRLAYFSLDATDVAITGYPEFLDY
                     LRALGQTTTLIKSASYLLHGNEFARVREVILDVSRFLVQDDTGLPYARLRKHGWEVRV
                     YGVYGVPIPPFEYAYQKSLAAVYEKQKPKPLPFRFGYQLNIVENRSNLMVARLPTVQG
                     RIGDGASVR"
     gene            3968..4843
                     /locus_tag="AC158Q_05320"
     CDS             3968..4843
                     /locus_tag="AC158Q_05320"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3339226.1"
                     /translation="MLASVLALGQAPAAHAMEPEIKSASFVTSDGVRLNVLEACAPGA
                     GASSPVIAFVPGWSMPAAIWRGQLLALGKTHCVAALDPRGQGESEIPPGGYTLDRRAL
                     DVREFVARYPRVVLVGWSLGALEALEYVHRHGAATVDALVLVDASVGEDPQPPPAGNF
                     LEALKRDRRAAIEDFVRASFRSPRAEPEIAALTDAALRMPLEASLSLFPRSVPREHWR
                     GIARSFPGPLLYIVSAQFAEQAESLKRNRPATQLAVFEEAGHALFVDEAARFSGLLVQ
                     FIGETAARSRPPRRN"
     gene            4892..6061
                     /locus_tag="AC158Q_05325"
     CDS             4892..6061
                     /locus_tag="AC158Q_05325"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3339227.1"
                     /translation="MSHWFEYFPGNHMWSQGMMFGIEMQTWGAAALGEIDQVGQKLKG
                     HVGDNEKWWAEWTAMARRIEGFGDAAEREGRRFTAGSYYLRAAVYYFCGERFIAPSER
                     KWDTYRNCLRCFRKGVERRYPDIERVEVPYIHDGAGTTLPAWLLKAGVKGPAPAVVMF
                     DGLDNAKELSVLFGGVEIARRGIHVLAIDGPGQGEALRLQGIPSRYDYEVPARAAYDY
                     LSRRPEIDPKRVGVMGFSMGGYYAPRAAAMEPRFAACLAWGGHWDYHESWVRRRRVME
                     SGGTKLSAPQFQLPWVLGMPDMDACMKKLENYRLAGVAERIRCPFYCIHGEDDNIVPL
                     EFAQKLFDAVGSKDKTLKVLAAHEGGSEHCQEDNRQVGANLVADWLAGRLGGRLG"
     gene            6082..6801
                     /locus_tag="AC158Q_05330"
     CDS             6082..6801
                     /locus_tag="AC158Q_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3339228.1"
                     /translation="MTRMLHCFSLRPAAALHALSVVLALSALAGCTPQAMLASALIPD
                     GTMSVFLGRLEKEEDGNRKRILQLEASKDWDGIAKFAEENLAKDRNNADWWFVAGYAH
                     SEAGRHQRAIECYGEMVRLAPDDMLGSTSLAQEYRATKQPARAVQTLNNALRTRKGTP
                     GIYFLLGESYADLDRDLPAAAAYREAVQIDGEFAKAWFGLGRAYARLGRREDYEKALT
                     ALGKLDSAMAQQLAELRPGPR"
     gene            complement(7016..7816)
                     /locus_tag="AC158Q_05335"
     CDS             complement(7016..7816)
                     /locus_tag="AC158Q_05335"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR3339229.1"
                     /translation="MHPMLNIAVKAARRAGGIINRAARNLDIIAVKEKAAKDFVSEVD
                     REAEQAIIRTLREAYPDHSILAEESGASGKSEYEWIIDPLDGTTNFLHGYPQYSVSIA
                     LRHRGTVTQAAIYDPSRNDLYTATRGRGAFLNDQRMRVSKRTHFGQCLIGTGFPFRQV
                     EHIDAYLAILRDMMTGAAGVRREGSAALDLAYVAAGRLDGFWEFGLAPWDMAAGALMI
                     SEAGGLVGDLAGESNYLETGNIVCGNPKIFVELLQMLSRHLTPALKRG"
     gene            7920..8663
                     /locus_tag="AC158Q_05340"
     CDS             7920..8663
                     /locus_tag="AC158Q_05340"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017778055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HIR3339230.1"
                     /translation="MAADPLKNIRVVLCETAHPGNIGAAARALKTMGLTQLVLVAPER
                     YPDPQAQWLAVGADDVLAGARVCASLDEALAGSAFTVACTARPREVAVPMVAAREAAA
                     RLVAVARSQPVALVFGNETYGLTGAEVGKCQLLATIPADPKFSSLNVAAAVQVLAYEL
                     RLAALGSTVRNSKGKPPEHATHDEIERFYEQLERSLLKAGFLNPRNPKKLMARLRRLF
                     GRAQLEQEEVNILHGVIKTLATPKSKRFR"
     gene            8824..9549
                     /gene="cysE"
                     /locus_tag="AC158Q_05345"
     CDS             8824..9549
                     /gene="cysE"
                     /locus_tag="AC158Q_05345"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0009001 - L-serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - L-cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HIR3339231.1"
                     /translation="MFERLKEEIAVVFDRDPAARNTWEVVTCYPGFHAMLFHRLAHPL
                     WNSGLKWLARLTSHLSRWLTGIEIHPGATIGRRFFIDHGMGVVIGETAEIGDDCTLYH
                     GVTLGGTSWNKGKRHPTLGKGVVVGAGAKILGPIMIGEGARIGSNAVVVKEVPPGATA
                     IGIPARIVEAEAAQNGRFAAYAVVKDLNDPVAKAMHELIGHCADTDRRIEEILAELKR
                     LGGRAEEHEPHKLDANSLNKMVD"
     gene            9640..10128
                     /gene="iscR"
                     /locus_tag="AC158Q_05350"
     CDS             9640..10128
                     /gene="iscR"
                     /locus_tag="AC158Q_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HIR3339232.1"
                     /translation="MRLTTKGRFAVTAMIDLAMHHGSGPVTLAEISGRQKISLSYLEQ
                     LFGKLRRHQLVESVRGPGGGYCLAKDMGQVSVTEIILAVDEPIDATQCGGKENCHDDR
                     KCLTHDLWAALNQRIFDYLGGVSLRQLVESQKAKEAKQPGVTPMHDMRETAGKRGTPV
                     SA"
     gene            10148..11293
                     /locus_tag="AC158Q_05355"
     CDS             10148..11293
                     /locus_tag="AC158Q_05355"
                     /inference="COORDINATES: protein motif:HMM:NF012488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HIR3339233.1"
                     /translation="MDHVYFDHNATTPVDERVLEAMLPYLRGRYGNASSRHELGTTAR
                     RAVDVAREQVAALVNVQPSQVIFVSGGTEANNLFIKGAAGYLKPTQIAVSAIEHPCVA
                     KPAQELARQGWKLHKLAVSRDGAVDSADVDRALKEPTGIVSVMLANNETGVIQDVAAV
                     AGRARAAKAWMHTDAVQALGKIPVDFAALGVHAMTISAHKLYGPKGAAALVMDKRLQL
                     KPIIHGGGHEQGLRSGTENVPAIVGFGAACALAGERMRELAPRLEAMRARFEQGLDEM
                     GAAVFGARATRIPNTTYFAFKGILGETLVIELDKAGYAVAPGAACSSANPEPSATLLA
                     MGVNPDIAHGAVRFSLGAGNTMPQIDEFLKALKSVVTRLRSLTAMAV"
     gene            11369..11704
                     /locus_tag="AC158Q_05360"
     CDS             11369..11704
                     /locus_tag="AC158Q_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/IscA family protein"
                     /protein_id="HIR3339234.1"
                     /translation="MKIELTENAAKQIQRQIAQRGRGIGLRVGVKDVGCSGFGYTYDY
                     ADEVRDGDLTFEAHDAKLVVDAKSLEFLNGSTLDYVKEGLKQVFKFTNPNVDATCGCG
                     ESFSVKEKA"
     gene            11884..>13087
                     /gene="sufB"
                     /locus_tag="AC158Q_05365"
     CDS             11884..>13087
                     /gene="sufB"
                     /locus_tag="AC158Q_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HIR3339235.1"
                     /translation="MSSVLENLVNQPYRHGFVTDIESDTAAKGLSEDTIRFISAKKGE
                     PEWLLEFRLKAYRHWLTMTEPKWPNVKYPKIDFQEIIYYSAPKPKKLASMDEVDPELL
                     KTFEKLGVPMNERAKLAGVAVDVIFDSVSVATTYKQKLADVGIIFCSISEAVREHPEL
                     VRKYMGSVVPAGDNYYAALNSAVFTDGSFCFIPKGVRCPMELSTYFRINTESSGQFER
                     TLIVAEEGSHVSYLEGCTAPKFDTNQLHAAVVELVALDNAEIKYSTVQNWYAGDENGV
                     GGIYNFVTKRGACRGANSKISWTQVETGSAITWKYPSCVLLGDNSIGEFYSVALTNHH
                     QQADTGTKMVHIGKNTRSRIISKGISAGQSNNSYRGLVKIGARAENARNYSQCDSMLI
                     GSKCGANTF"
BASE COUNT         2256 a         4097 c         4512 g         2222 t
ORIGIN      
        1 tttgaaatgt atttcttggc ggtcttggcg tcttggcggt taatttattc atcaagctgc
       61 ttgcgcaact gatacagcag ctccagtgct tccttcggcg tgagcgcgtc gggatcgagc
      121 tcgcgcaggc gcttcaccac cgcatgttcc tccgacgcgg gcgcgtcctg cgcgtttttc
      181 gccccggccg cgaagaggtc gcgctgcggg ccgccggcga ggccgtgctc ctcgagctcg
      241 gcgagccgcc tgcgcgcagc gcggatcacg gcgccgggaa cgccggcgag tgccgccact
      301 tccagcccgt agctctggct ggcagggcct tcctcgacgt tgtgcaggaa gacgatgctg
      361 tccttgtgct cgaccgcgtc gaggtggacg ttcgcggcct ggcggtagtc cgcggcgagc
      421 cgtgtcattt cgaagtagtg cgtggcgaag agcgtgaggg agcgggtctt ctcgacgagg
      481 tgccgcgcga tcgcccacgc aagcgcgagc ccgtccaggg tggacgtgcc gcggccgatc
      541 tcgtccatca gcaccaggct ttgcggcgtg gcgtggtgca gggtgaacgc tgcctcagtc
      601 atctccacca tgaacgtgga gcgcccgccg gcgagatcgt cagacgcgcc gatgcgggtg
      661 tagatcgcat cgagcgggcc caggcgcacg cgctgcgccg ggacgaacga gccgcagtgc
      721 gcgagcagca cgatcagggc gacctgccgc atgtaggtgg acttgccgcc catattgggg
      781 ccggtgacga tcagcatgcg gcgctcgtcc gcgaggcgcg cgccattggc gatgaagttc
      841 tcgacctgtt gctccaccac cgggtgccgg ccggcgtcga tctcgatcag cggctcgtcg
      901 gcgaaatccg ggcggacgta atcgagcgcc gccgcccgct cggcgaaggc ggacaggaga
      961 tcgagctcgg cgagcgcgcc ggccgcgatc tgcagccgcg ggaggtaagg agcgagatcg
     1021 tcgagaagca ggtcgtagag cagcttctcg cgcgcgagcg cgcggtcctg cgccgacagc
     1081 gccttgtcct cgaacgcctt cagctcgggc gtgatgtagc gctcggcgtt cttgagcgtc
     1141 tggcggcgcc ggtaatcgtc cgggaccttt cccgcttgcg cgcgccccac ctcgatgtag
     1201 aagccgtgca cccggttgta ctcgaccttg agattggcga tgccggtgcg cgcgcgctcg
     1261 cgggtctcga gttcgaccag gaattcgccg caattcgtct ggatcgccct cagttcgtcg
     1321 agctcggcat cgtagccgtc ggcgatcacg ccgccctcgc gcagcaccgc cgccggctcg
     1381 ggcttgatcg cggccgcgag cagcgctgcc agccggccct gcggcgcgag cgcgccagcc
     1441 agctccgcga gcttcgaccc cacaagcgcg gcgagggtgg catgcagtcc cggcatcagc
     1501 ccgagcgttt ctcgcagccc ggacaaatcg cgcgggcggg cggatttcaa cgcgatgcgc
     1561 gccgtgatgc gctcgacatc cacgcactga cggagcgttt catgcaggcc tcggtagggt
     1621 gccgcgccat cgcgggcaat cagcgcttcc accgcgtcga gccgggcgga gagcacggcg
     1681 cggtcgcgca ccgggtgatg cagggtctgg cgcagcaggc ggctgcccat gccggtcgcg
     1741 caggtgtcga gcagcgagta gagggtcggc gccggctcgc cgcgtatcgt ctcggtcagt
     1801 tccaggttgc ggcgggtggc cgggtccatg cggacccagg cgcgctcgtg ctcgaccgtc
     1861 agcgatttga cgtgggcgat cgcggtgccc tgggtggcgc gggcgtattc cagcagcgct
     1921 ccggcagcgg aaaccgccac cgtcaggtcg gaggcgccga aaccggacag gtcgtgcgtt
     1981 tcgaactgac gggcgagggc ccgccgggcg gcatcgagat cgaactgcca gagaggcagg
     2041 cgtttgaccg cagccgccgc gccgttcaga aaatccgccg cggtatcctc cgcaaccagg
     2101 atttccgagg gggcgagacg ctcgagctcg gcggccaggt tggcggcggc cgtttccatc
     2161 gcggtgaaac ggccggaggc gagcgagagc caggcgagcc ccaccgtgcc ccgctgcggg
     2221 cagagcgcga gcagcaggtt gtcgcgtttt tcgtcgagga gggcggcgtc ggtcagggtg
     2281 ccgggcgtga ccacgcgcgt gacctgccgc tcgaccggcc ctttcgaggc gccggggtcg
     2341 ccgatctgct cgcagatcgc caccgattcg ccgagcttca ccagtttcgc caggtactgc
     2401 tcgaccgagt ggtgcggaac gcccgccatc ttgatcgctt cgccggcgga ttcgccgcgg
     2461 cgcgtgagcg tgatatcgag caggcgcgac gcgcgctcgg cgtcgtcgta gaacagctcg
     2521 tagaagtcgc ccatgcggta gaacagcagc ttgtccgcgt gctgcgcctt gatgcgcagg
     2581 tactgctgca tcatgggggt atggcgttcc agggattgct gcatgcggac cgggcttcct
     2641 tgcggactgg cgctgctatt ttgacacgtt gaccgtttgc attgaccgga cggcgccgtg
     2701 ccgggcagaa atggcgcatg ctgctagaat cccggcatgc aaagccacct gacgacattt
     2761 cgggcggtcc ggctgctggc ggcagctgcg gcctggacgg tttttttcac tggccagctc
     2821 gcctggttcg ccggcacggc gcagggcgcc gcagggcccg atcccggccc acagccgctc
     2881 ctcgattcca ccgcccggct gatcgccggc atggagccga cgcatccgga tcacgcagag
     2941 cttacccgca gcaaggaatg gcagcagcat agcggcgcaa tccagtcttc atggatccgc
     3001 gtccgggacg ggcagctggc agccgtgacc gcctggcgcc aggccgagct tcctccgggc
     3061 tgtcccgtgg gccagaccct gttctacccg ttcagcggtc ccgacttcct caatgcccac
     3121 tggctgttcc cgggctgcga gaacctggtg atgttcggcc tcgagcggat cggcgtggtg
     3181 cccgatgtcg gatcgatgtc gagccaggaa ttcacgcgcc tgctcacgaa cacgcgcagt
     3241 ttcatggtca acctgttcgc gcgcaactac ttcgtcacgg acaccatgca aaaggatctc
     3301 cgttccgcgg agcagctgcg cggggtggtg cccgtgttca tggtcctgat ggcgctgtcc
     3361 ggctgcgaag tgctccgggt cgcgccgctc gagctggagc gcgcaccggg cgccagggca
     3421 cccgccaagg ggcggaaacg cgagctgagg ggcgtcacga tcgaataccg ggtgaccggt
     3481 acgtcgcagg tgcggcgcct tgcctatttt tccctcgacg cgacggatgt cgccatcacg
     3541 ggctacccgg agttcctcga ctacctgcgc gcgctggggc agacgacgac gctgatcaag
     3601 tcggcgtcgt acctcttgca tggcaacgag ttcgcgcgcg tgcgcgaggt gatactcgac
     3661 gtgagccggt ttctcgtgca ggacgacacc gggctgccgt acgcccggct gcgcaagcac
     3721 ggctgggagg tgcgcgtcta tggggtctac ggcgttccga ttccgccgtt cgagtacgcc
     3781 taccagaagt cgctggcggc cgtctatgag aaacagaaac cgaagccatt gccgtttcga
     3841 ttcggttacc aactgaacat cgtggagaac cgctcgaacc tgatggttgc gcggctgccc
     3901 actgtccagg gacgcatcgg cgacggcgcg agcgtgcgct agcgtgcctc cccgtacccg
     3961 ggccgccttg ctggctagcg tcctggcgct cgggcaggcg cccgccgcac acgcgatgga
     4021 acccgagatc aagagcgcga gcttcgtaac atccgacggt gtgcgcctca acgtcctgga
     4081 ggcttgtgcg ccgggcgccg gcgcgagctc gccggtgatc gcgttcgttc ccggctggtc
     4141 gatgccggcg gcgatctggc gcgggcagct tcttgccctg ggaaagacgc attgcgtcgc
     4201 cgcgctcgat ccccgcggac agggggaatc cgagattcca ccgggcggct acactctcga
     4261 ccggcgggca ctggacgtgc gcgaattcgt cgcgcgctac ccgcgcgtcg tgctggtggg
     4321 atggtcgctc ggcgcgctgg aagcgctgga atacgtgcat cgccatggcg ccgccacggt
     4381 cgatgcgctg gtgctggtcg atgcctcggt cggggaggac ccgcagccgc cgccggccgg
     4441 taacttcctg gaagcgctca agcgcgaccg ccgcgccgcc atcgaggatt tcgtgcgcgc
     4501 cagcttccgc tcgccgcgcg cggagcccga gatcgccgcg ctgacggacg ccgcgctgcg
     4561 catgccgctc gaggcgagcc tgtcgctctt cccgcggtcg gtgccgaggg aacactggcg
     4621 cggcatcgcc cggtcgtttc cggggccgtt gctgtacatc gtcagcgcgc aattcgcgga
     4681 gcaggcggaa tcgctgaagc ggaacaggcc ggcgacgcaa ctggcggtct tcgaagaagc
     4741 cggccatgcc ctgttcgtgg acgaggcggc gaggttcagc ggcctgctcg tgcagttcat
     4801 cggcgagacg gcggcccgat cacgcccgcc gcggcgcaat tgaacggttt cgccgcctcg
     4861 tctatccagt cagtctgaca gcaggggcgc catgagccat tggttcgagt atttccccgg
     4921 caaccacatg tggtcgcagg gcatgatgtt cggcatcgag atgcagacct ggggcgcagc
     4981 ggcgctgggc gagatcgacc aggtcgggca gaagttgaag ggccatgtcg gcgacaacga
     5041 aaaatggtgg gccgaatgga ccgcgatggc gcggcgcatc gaaggcttcg gcgatgcggc
     5101 cgagcgtgaa ggccgccggt tcaccgcggg cagctattac ctcagggcgg ccgtctacta
     5161 cttctgcggg gagcgcttca tcgcgccgtc ggagcgcaag tgggacacct atcgcaactg
     5221 cctgcgctgt ttccgcaagg gtgtcgagcg ccgctatccg gatatcgagc gcgtcgaggt
     5281 gccctatata cacgacgggg cgggcacgac gctgccggcg tggctgctca aggccggcgt
     5341 gaaggggccg gcaccggccg tcgtgatgtt cgacgggctc gacaacgcga aggaactctc
     5401 ggtgctgttc ggcggggtgg agatcgcccg ccggggcatc cacgtgctgg cgatcgacgg
     5461 gccggggcag ggtgaggcgc tgcgcctgca gggcatcccg agccgctacg actacgaagt
     5521 gccggcgcgg gcggcctatg actacctgtc acggcggccg gaaatcgatc cgaagcgggt
     5581 cggggtgatg ggcttcagca tgggcggtta ttacgcgccg cgcgcggccg cgatggagcc
     5641 gcgtttcgcg gcgtgcctcg cctggggcgg ccactgggat tatcacgagt cgtgggtgcg
     5701 ccggcgccgg gtcatggaaa gcggcgggac gaagctgtcc gcgccgcagt tccagctgcc
     5761 gtgggtgctc ggcatgccgg acatggatgc ctgcatgaag aagctcgaga actaccgcct
     5821 ggcgggcgtc gccgagcgca tccggtgccc gttctactgc atccatggcg aggacgacaa
     5881 catcgtgccg ctcgaattcg cgcagaagct cttcgacgcg gtcggctcga aggacaagac
     5941 gctgaaagtg ctcgccgcgc acgaaggcgg cagcgagcac tgccaggagg acaaccgcca
     6001 ggtcggcgcg aacctcgtcg ccgactggct ggccgggcgc ctcggcggcc ggctcgggtg
     6061 agccggtttg ccgcggttac aatgacccgg atgctgcact gcttctcact tcgtccggcg
     6121 gctgcgctgc atgcgctaag cgtggtattg gcgttgtcgg cgctcgccgg ctgcacaccg
     6181 caggcaatgc tggcgagcgc attgattccc gacgggacga tgtccgtctt tctgggccgt
     6241 ctcgaaaagg aggaggacgg caatcgcaag cgcatccttc agctggaagc gagcaaggat
     6301 tgggacggca tcgcgaaatt cgccgaagag aacctggcca aggaccgcaa caatgccgac
     6361 tggtggttcg tcgcgggtta cgcgcactcg gaagccggca ggcaccagcg ggcgatcgaa
     6421 tgctacggcg agatggtgcg gcttgcgccc gacgacatgc tggggtccac ctcgctcgcg
     6481 caggagtacc gcgccaccaa gcagccggcg cgcgcggtgc agacgctcaa caacgcgctg
     6541 cgcacccgga aaggcacgcc gggaatctac ttcctgctgg gggaaagcta tgccgatctc
     6601 gaccgcgacc tgccggcggc cgcggcctat cgcgaggcgg tccagatcga cggcgagttc
     6661 gcgaaggcct ggttcgggct ggggcgggcg tatgcccggc tcggccgcag ggaagattac
     6721 gagaaggcgt tgacggcgct cggcaagctg gattccgcga tggcgcagca actcgccgaa
     6781 ctgcgccccg gcccgcgctg accaaaggcc ataaattagc cgccaagacg ccaagaacgc
     6841 caagaaaaac ggaacaataa ataaattcag aacggcttgc gctttgcgag ctcctcaatc
     6901 tggagcactc gtgcgtcgct actccagagc cgtcaatcct gtttatgttt tttgtactga
     6961 tttccttggc gttcttggcg tcttggcggc tatttgtaag cctgttctta ccggttcagc
     7021 cgcgtttgag cgcgggcgtg agatgccgcg acagcatttg cagcaactcg acgaagatct
     7081 tcgggttgcc gcagacgatg ttgccggtct cgaggtagtt gctctcgccg gcgaggtcgc
     7141 ccacgagtcc gccggcttcg gagatcatca gcgcgccggc ggccatgtcc cagggcgcga
     7201 ggccgaattc ccagaaaccg tccagacggc ccgccgcgac gtaggcgaga tcgagcgcgg
     7261 ccgagccttc gcggcgcacg ccggccgccc ccgtcatcat gtcgcgcagg atcgccaggt
     7321 aggcgtcgat gtgctccacc tggcggaacg gaaagcccgt tccgatcagg cattgcccga
     7381 agtgcgtgcg cttgctgacg cgcatgcgct ggtcgttgag gaaggcgccg cgcccgcgcg
     7441 tggcggtgta gagatcgttg cgcgaggggt cgtagatcgc cgcctgcgtg acggtgccgc
     7501 ggtgtcgcag cgcgatcgac accgagtact gcgggtagcc gtggagaaaa ttggtggtgc
     7561 cgtcgagggg gtcgatgatc cactcgtatt ccgattttcc ggaagcgccg ctttcctctg
     7621 ctagaatcga atgatcggga taggcctcgc gcagcgtgcg gatgatcgcc tgctccgctt
     7681 cccggtccac ttccgagacg aaatccttcg cggccttttc cttgacggcg atgatgtcga
     7741 gattgcgggc cgcgcggttg atgatgccgc cggcgcggcg cgcggccttg accgcgatgt
     7801 tgagcatcgg atgcatgatt ggcagtaatg agtgatgagt gatgagtgat gaacgggaaa
     7861 ctcgctactg catcccacgt caaatcgcgg atcgttttcc gcgcccgcta ttgtacgtga
     7921 tggctgccga tccgctgaag aatattcgcg tggtgctgtg cgaaacggcg cacccgggca
     7981 atatcggtgc ggcggcgcgg gcgttgaaga cgatggggct cacgcaactg gtcctcgttg
     8041 cgcccgagcg ctatccggat ccgcaagccc agtggctggc ggtcggcgcg gacgacgtgc
     8101 tcgccggcgc acgcgtgtgc gccagcctgg acgaggcgct ggcgggatcg gcgttcacgg
     8161 tggcctgcac ggcgcggccg cgcgaagtgg cggtgccgat ggtcgccgcg cgtgaggccg
     8221 cggcgcgtct cgtcgcggtg gcccgctcac agccggtcgc gctggtattc ggcaacgaaa
     8281 cctacgggct gaccggagcc gaagtcggca agtgccagtt gctcgcgacc atccccgccg
     8341 accccaagtt ctcgtcgctc aacgtcgcgg cagcggtcca ggtgcttgcc tacgagttgc
     8401 gcctagcagc gctgggttcg accgtgcgca acagcaaggg gaaaccgccc gagcatgcga
     8461 ctcacgacga aatcgagcgc ttctacgagc agctcgagcg cagcctgctc aaggccggct
     8521 ttctcaatcc gcgcaatccg aagaagctga tggcgcggct gcggcggctg ttcggccgcg
     8581 ctcaactcga gcaggaggag gtcaacatcc tgcacggcgt gatcaaaacg ctggcgacac
     8641 caaaatcgaa gcgtttccgc taatcctctc gggtaggcat gcgcgttgaa cggcgtatcc
     8701 cgtccgttcc aacaggcata gccggatcgg ctggacggta tcgtcctggc cgggccccaa
     8761 caaaggttga ccaatttact aggctatcgt aagctgcgcc cccttatcct tgatttgtaa
     8821 acgatgtttg agcgactgaa agaggaaatc gcggtcgttt tcgaccgcga cccggcggcc
     8881 cgcaatacct gggaggtagt gacttgctac cccgggttcc acgcgatgct gttccaccgc
     8941 ctggcccacc ccttgtggaa ttccgggctc aagtggctcg ctcggctgac ttcccacctg
     9001 tcgcgctggc tgaccggcat cgagattcat cccggcgcca cgatcgggcg gcgcttcttc
     9061 atcgaccacg gcatgggcgt ggtgatcggc gagaccgccg agatcggcga cgactgcacg
     9121 ctctatcacg gcgtcacgct cggcggcacc tcctggaaca agggcaagcg ccatcccacc
     9181 ctgggcaagg gtgtggtggt gggcgccggc gcgaagatac tcggaccgat catgatcggg
     9241 gaaggcgcgc gcattggttc gaacgcggtg gtggtgaaag aggttccgcc gggcgcgacg
     9301 gcgatcggaa ttccagcgcg catcgttgaa gcggaggcgg ctcagaacgg gcgctttgcc
     9361 gcctatgcgg ttgtgaagga cctgaacgac ccggtggcga aggcgatgca cgagctgatc
     9421 ggccactgtg ccgacacgga ccgccgcatc gaggagatcc tggcggaatt gaagcgcctg
     9481 ggcggccggg ccgaagagca cgaaccccac aagctcgatg cgaactctct caataaaatg
     9541 gttgactaaa ttactcggga taagtatagt tgactaattt agtggtgtat tagattgtat
     9601 attaactgtt tgattaaatg aagttttaag aggtggccca tgagactgac gacgaaggga
     9661 cgctttgcag tcacggcgat gatcgacctg gccatgcacc atggcagcgg gccggtgacg
     9721 ctggccgaga tcagcggccg gcagaagatc tcgttgtcct acctcgagca gctgttcggc
     9781 aagctgcggc gccaccagct ggtggaaagc gtgcgcggcc ccggcggcgg ctattgcctc
     9841 gcgaaagaca tggggcaggt gtccgtcacc gagatcattc tcgccgtgga cgagccgatc
     9901 gatgccacgc aatgcggcgg caaggaaaac tgccatgacg accgcaagtg cctgacccat
     9961 gacctgtggg ccgcgctgaa ccagcgcatc ttcgattacc tcggcggcgt gagcttgcgc
    10021 cagctggtgg aaagccagaa agccaaggag gcgaagcagc ctggcgtcac ccccatgcac
    10081 gacatgcgcg agacggctgg caagcggggc acgccggtat ccgcgtagcc gttgcgcgtt
    10141 tcccgttatg gaccacgttt acttcgatca caacgcgacg acacccgtgg atgaacgggt
    10201 gctcgaggcg atgctgccat acctgcgcgg ccgctacggc aacgcgtcca gccggcacga
    10261 gctcggcacg actgcgcgca gggcggtgga tgtcgcgcga gagcaggtcg ccgcgctcgt
    10321 caacgtgcag ccctcgcagg tgattttcgt ctccggcggc accgaagcga ataatttgtt
    10381 catcaagggc gcggcgggat acctgaagcc gacgcagatc gccgtcagcg cgatcgagca
    10441 tccctgcgtg gcaaagccgg cgcaggagct ggcgcggcag ggttggaagc tgcacaagct
    10501 ggccgtcagc cgggacggcg cggtcgattc cgccgacgtg gaccgggcgc tgaaggagcc
    10561 gaccggcatc gtgtcggtga tgctcgcaaa caacgagacc ggcgtgattc aggacgtcgc
    10621 agcggtcgct ggccgcgcgc gcgccgcgaa agcgtggatg cacaccgacg cagtgcaagc
    10681 gctcggcaag atacccgtgg acttcgccgc gctcggggtg cacgcgatga ccatctccgc
    10741 gcacaagctc tacgggccga aaggcgccgc ggcgctggtg atggacaagc ggctgcagtt
    10801 gaagcccatc atccacggcg gcggtcacga gcaggggctg cgttccggta ccgagaacgt
    10861 gccggcgatc gtcggattcg gcgcggcctg cgcgctcgct ggcgagcgga tgcgcgagct
    10921 cgcgccgcgg ctggaggcga tgcgggcgcg gttcgagcag gggctcgacg aaatgggcgc
    10981 ggccgtcttc ggcgcgcgcg ctacgcgcat acccaatacg acgtacttcg cgttcaaggg
    11041 catcctgggc gagacgctgg tgatcgagct cgataaggcc ggttacgcgg tggcgcccgg
    11101 cgccgcttgc tcgagcgcga accccgagcc ttcggcgacc ctgctcgcga tgggcgtgaa
    11161 ccccgacatc gcgcacggcg cggtgcgttt ctcgctcggc gcggggaata cgatgccgca
    11221 gatagatgaa tttctgaagg ctctcaagag cgtcgttacg cgcttgagaa gtttgaccgc
    11281 tatggcggtc tgacccccac cttatccctc ccccttgcca agggggaggg ttggggaggg
    11341 ggtgatgacg agtgacgaga ggcttgctat gaagatagaa ctgacggaaa acgcggcgaa
    11401 gcagatccag aggcagattg cccagcgcgg gcgcggaatc gggctgcgcg tgggcgtgaa
    11461 ggacgtgggc tgctccggtt tcggctacac ctacgactac gcggacgagg tgcgtgacgg
    11521 cgatctcacg ttcgaggcgc acgacgcgaa gctcgtggtg gacgcgaaga gcctggagtt
    11581 cctgaacggc tcgacgctcg actacgtgaa agagggcctg aagcaggtgt tcaagttcac
    11641 caacccgaac gtggacgcga cctgtggctg cggcgaatcc ttcagcgtca aggaaaaagc
    11701 gtagaaccgg aaattagccg ccaagacgcc aaggccgcca agaagagcag gaaatatatc
    11761 gaaagactga taatgcgatc gggaaacgtt gcggaacgaa agcgatgaaa gcggtgtttt
    11821 taattctgga tctggtttcc ttggcgttct tggcgtcttg gcggctattt tgaggtttta
    11881 gacatgagca gcgtgctcga aaacctagtc aaccagccct accggcacgg gttcgtgacc
    11941 gacatcgagt cggataccgc ggcgaagggg ctgtccgagg acaccatccg cttcatttcg
    12001 gcgaagaagg gcgagccgga atggctgctg gagttccggc tgaaggccta tcgccactgg
    12061 ctcaccatga ccgagccgaa gtggccgaac gtgaagtacc cgaagatcga cttccaggag
    12121 atcatctact actccgcgcc caagccgaag aagctcgcga gcatggacga agtggacccg
    12181 gaactcctca agaccttcga gaagctgggc gtgccgatga acgagcgggc gaagctcgcc
    12241 ggggtcgcgg tggacgtcat cttcgactcg gtatcggtcg cgaccaccta caagcagaag
    12301 cttgccgacg tgggcatcat cttctgctcg atctcggaag cggtgcgaga gcatcccgag
    12361 ctggtgcgta agtacatggg cagcgtggtg ccggcgggcg acaactacta tgcggcgctc
    12421 aactccgcgg tgttcaccga cggctcgttc tgcttcatcc cgaagggcgt caggtgcccg
    12481 atggagctgt cgacctattt ccgcatcaac acggaatcct ccgggcagtt cgagcggacc
    12541 ctcatcgtcg ccgaggaagg ctcccacgtc tcctacctgg agggctgcac ggcgcccaag
    12601 ttcgacacca accagctgca cgccgcggtg gtggagctgg tggcgctgga caacgccgag
    12661 atcaagtact ccaccgtgca gaactggtac gccggcgacg agaacggcgt gggcggcatc
    12721 tacaacttcg tgaccaagcg cggcgcctgc cggggcgcca actccaagat ctcctggacc
    12781 caggtcgaga ccggctcggc gatcacctgg aagtacccct cctgcgtgct gctcggcgac
    12841 aactcgatcg gcgagttcta ctcggtcgcg ctcaccaacc accaccagca ggccgacacc
    12901 ggtaccaaga tggtgcacat cgggaagaat acccgcagcc gcatcatatc caagggcatt
    12961 tcggcggggc agtcgaacaa cagctaccgc ggcctggtca agatcggggc gcgcgcggag
    13021 aacgctcgca actactcgca gtgcgattcg atgctgatcg gttcgaagtg cggcgccaac
    13081 actttcc
//
LOCUS       DBNGSG010000201         6316 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2442739, whole genome shotgun sequence.
ACCESSION   DBNGSG010000201 DBNGSG010000000
VERSION     DBNGSG010000201.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6316)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6316)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6316
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2442739"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..1031)
                     /locus_tag="AC158Q_05370"
     CDS             complement(90..1031)
                     /locus_tag="AC158Q_05370"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR3339236.1"
                     /translation="MPASRKSPASRAAPKPVSGSAARAYQDILYEVKDQVAWVTINRP
                     RVLNAFREQTLDEMIDALKSTREDPTIVCAVVTGTGDKSFSAGGDFFAMKRLNFTNAY
                     MWNDRMLGLAMTIRGLPIPVIAMVNGWCMGGGHELALWCDLVIASENAVLGQTGAKVG
                     ACPTVGATQYLPRLIGERLAREMIFCARRFGAKEAVEIGLINKCVPQKDLLKETLKWC
                     ETMKGHSALTIRMTKKSLNFESDNLYSSWQHGMELLAHVWGSDEAKEGMNAFLAGRPP
                     NFQKFRLRAKKELAQYVDGFNRDMNAPPDMRRKKAKS"
     gene            1179..2171
                     /locus_tag="AC158Q_05375"
     CDS             1179..2171
                     /locus_tag="AC158Q_05375"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3339237.1"
                     /translation="MRKADRFLLALCALAFPALACAQAPSTSLGQAYPAKPIRIIVPF
                     APGGPTDTHSRWAGQQLTAAFGQPVIVDNRGGAGGVIGTEAVAKAAPDGYTLLGGNPG
                     PLTIATSVRKQLNYDGIRDFAPITLIALSASCMCAHPTVPAKALREFIALAKKNPGKI
                     NYATPGIGTVGHFAIESFAARAGIKMTMVPYKGAALYVVDLMSGNIDFAQVQIFQAAP
                     YVRDGRMRALGVTANKRSPHLPDVPTASEQGLTGWSSYNWNGILAPAGTPAAIIERIH
                     AALSRPLNDPVTRKQLQDVGYEVSGDGPAPFAAFLKSETEKWAQVAKSAGIPRQ"
     gene            2283..3458
                     /locus_tag="AC158Q_05380"
     CDS             2283..3458
                     /locus_tag="AC158Q_05380"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3339238.1"
                     /translation="MADKKDPAYFMYFPGNYRWSAAFINMIGSIAYGGAEIGELHKIG
                     RLLKDKGPEDDEAWFHACVNVAGGVRAYAEKWDKSGHRHSAAHAYLRACNYYQMAERF
                     RTPKDKTGLDAYRKGVDCFHRHAALTDLKIEIVEVPYENGSLPGYFVHAENAKVGRVP
                     CVVFFDGLDVTKEIQFVRGVPDLVKRGIACLVMDAPGTGEAIRFRNYYLRHDYEVAGS
                     ACVDWLEKRAEVDPKKIGIVAISLGGYYAPRCAAMEPRFAACIAWGAQWDYHATWKKR
                     IDAAYKTSLSVPGHHIEWIMGAKSVDEALAKLEPFKLDGVMQKMRCPFLLVHGADDEQ
                     IPLADAQKQFDACGSRDKTFRVFTAEEGGAQHCQRDYLTLVVAEMWNWFEDKLLRDK"
     gene            3552..3950
                     /locus_tag="AC158Q_05385"
     CDS             3552..3950
                     /locus_tag="AC158Q_05385"
                     /inference="COORDINATES: protein motif:HMM:NF017913.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HIR3339239.1"
                     /translation="MIERIESFGGILVKGFHLLALFVIGGTVVWSAAHFYIETMQQGY
                     ARLHDILLLFIYLEIGAMVGIYFKTHRLPVRFLIYVAITALTRLLTIDAKDLSNEQIL
                     TVTGAILVLTLAALAVEFSAARFQSPEQDV"
     gene            3954..4421
                     /locus_tag="AC158Q_05390"
     CDS             3954..4421
                     /locus_tag="AC158Q_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR3339240.1"
                     /translation="MGTVESRLAALGLTLPDAPSPAANYLPYTMAKGLIFIAGQAPVI
                     DGKYQSVGRVGAEVTLEAAQAAARLCGLNVLAQVKAAVVGDWSRVRSCVRLCGYVSSA
                     PGFHDQPRVLDGASDLMVQALGDAGKHARSALGVFSLRGNVPVVIDAIFELAD"
     gene            complement(4561..5037)
                     /locus_tag="AC158Q_05395"
     CDS             complement(4561..5037)
                     /locus_tag="AC158Q_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3339241.1"
                     /translation="MAKVTIKVNGKNYTKSVEDRMLLVNFLRDELKLTGTHVGCDTSQ
                     CGACTVHVNGRAVKSCTLLAAQANGATVVTIEGMAKGGKLHPIQQAFSECHGLQCGFC
                     TPGMIMATAGLLKDKPNPTDDDIYKGLEGNLCRCTGYINIVKAVKRSAKLLRGKKP"
     gene            complement(5128..5931)
                     /locus_tag="AC158Q_05400"
     CDS             complement(5128..5931)
                     /locus_tag="AC158Q_05400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR3339242.1"
                     /translation="MYALNYVKAKSVKEAAAFLASHPEAKLLSGGMTLLPTLKQRLAR
                     PSHLVDIGDLAELRRIEAKGGRLVIGAGAKHHEVATSAVVKKAIPALAGLASAIGDPQ
                     VRNRGTIGGSVANNDPAADYPAAVLGLNATVVTTKRQIAADQYFQGLFTTALEEGEIV
                     VRVEFPLPKRAAYSKFHHPASGYAMAGVFIAQTAEGVRVAVTGAGPGVFRWKEAEAAL
                     AKGLKPAALEGVKLDSREFNSDIHADRDYRANLVRVMAKRALAKLSGKK"
     gene            complement(5934..>6316)
                     /locus_tag="AC158Q_05405"
     CDS             complement(5934..>6316)
                     /locus_tag="AC158Q_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HIR3339243.1"
                     /translation="DDVGTVINHEVVEGQLHGGVAMGAGQIFGEQVVYDRGTGQLLTG
                     SFMDYFMPRAGLVPAIRGEEHPTPSKVSPLGVKGVGESGCTGSLPALANAVMDALRPL
                     GIQHLDMPLTPSRVWHAINAVRKG"
BASE COUNT         1072 a         2054 c         2007 g         1183 t
ORIGIN      
        1 gattattgtc ctgattttct tggcgccttg gcgtcttggc ggttcgaatt tcgaaatttt
       61 tcctttgtgt cctttgtgtc ctctgtgtct cagcttttcg ccttcttgcg ccgcatgtcc
      121 ggcggcgcgt tcatatcgcg attgaaaccg tcgacgtact gcgccagctc cttcttcgcg
      181 cgcaggcgga atttctggaa gttgggcggc cgccccgcga ggaaggcatt catgccctcc
      241 ttcgcctcgt ccgagcccca tacgtgcgcg agcagctcca tgccgtgctg ccacgacgag
      301 tacaggttgt cggactcgaa gttgagcgac ttcttggtca tgcggatcgt aagcgcgctg
      361 tgacccttca tggtttcgca ccacttgagc gtctctttca gcaggtcctt ctgcggcacg
      421 cacttgttga tgagcccgat ctcgaccgcc tctttcgcgc cgaagcggcg cgcgcagaag
      481 atcatctcgc gcgcgaggcg ctcgccgatg aggcgcggca ggtactgggt cgcgccgacc
      541 gtcgggcagg cgccgacctt ggcgccggtc tgccccagca ccgcgttctc ggaggcgatc
      601 accaggtcgc accacaacgc gagttcgtgg ccgccgccca tgcaccagcc gttcaccatc
      661 gcgatcaccg ggatcggaag cccgcggatc gtcatggcga gcccgagcat gcggtcgttc
      721 cacatgtacg cattggtgaa gttgagccgc ttcatggcga aaaaatcgcc gcccgcggag
      781 aacgacttgt cgcccgtgcc ggtcaccacc gcgcacacga tggtcgggtc ctcgcgcgtg
      841 gacttgaggg catcgatcat ctcgtcgagc gtctgctcgc ggaaggcgtt cagcacgcgc
      901 ggccggttga tggtgaccca cgcgacctgg tccttcactt cgtagagaat gtcctggtag
      961 gccctcgctg cggatccgga caccggtttc ggcgcggccc tggatgccgg actttttctg
     1021 gatgcgggca tgtcgttcct cgattgaatc agctggttgc gcgggcgatg cgaagcgcat
     1081 gaagtttacc attgaggccg cgcacccgct gcgctaccat cgcgtgcata acacgcaaaa
     1141 ccgtggccgg gacacgttcc atttcaagga ggagagccat gaggaaagcc gaccgttttt
     1201 tgctcgcgct ctgcgcgctt gcgttccccg cgctggcatg cgcccaggcc ccttcgacca
     1261 gcttgggaca ggcttacccg gcaaagccga tccggatcat tgttccattt gcgccgggcg
     1321 gccccacgga tacgcattcg cgctgggcgg gccagcagct tacggccgcg ttcggccagc
     1381 cggtgatcgt cgataaccgc ggcggtgcgg gcggggtgat cgggaccgag gcggtcgcca
     1441 aggcggcgcc cgacggctat acgctgctcg gaggcaaccc ggggccgctg acgatcgcga
     1501 cgagcgtgcg caagcagctc aactacgacg gcattcggga cttcgcgccg atcacgctga
     1561 tcgcgttgag cgcgagctgc atgtgcgcgc atccgacggt gcccgcgaaa gcgctgcggg
     1621 aattcatcgc gctggcgaag aaaaaccccg gcaagatcaa ttatgcgacc ccgggcatcg
     1681 gcaccgtcgg ccatttcgcg atcgagagct tcgccgcgcg cgccggcatc aagatgacga
     1741 tggtcccgta caagggcgcc gcgctatacg tcgtcgacct gatgtcgggc aacatcgatt
     1801 tcgctcaggt ccagatcttc caggcggcgc cttacgtccg cgacggcagg atgcgcgcgc
     1861 tcggggtgac ggcgaacaag cgttccccgc acctgcccga cgtgccgact gcgtccgagc
     1921 agggcctgac ggggtggtcg agctacaact ggaacggcat cctcgcgccg gcaggaacgc
     1981 ccgcggcgat catcgagcgc atacatgcgg cgctgtccag gcccctcaac gacccggtca
     2041 cccgcaagca actgcaggat gtcggctacg aagtctcggg cgatggcccg gccccgttcg
     2101 cggcgttcct caagtcggag acggagaaat gggcgcaggt cgcgaagtcg gcgggcatac
     2161 ccaggcagta ggacgagtgt cgagcattga gtatcgaggg aaaacgccgc cacacagtct
     2221 ccggctcgcc tgatcgccac tgaccactcg acacccgaca ctcaagagag gaagaaaaat
     2281 ccatggcaga caagaaagac cccgcctact tcatgtactt cccggggaac taccgctggt
     2341 cggcggcgtt catcaacatg atcggctcga ttgcctacgg cggggccgag atcggcgaac
     2401 tgcacaagat cggccgcctg ctgaaggaca agggtccgga ggacgacgaa gcctggttcc
     2461 acgcctgcgt gaatgtcgcc ggcggcgtgc gcgcctatgc cgagaaatgg gacaagtccg
     2521 gccaccgcca ttccgcggcg cacgcctacc tcagggcatg caactactac cagatggccg
     2581 agcgcttccg cacgcccaag gacaagaccg ggctcgacgc ctaccgcaag ggggtcgact
     2641 gcttccaccg gcacgccgcg ctgaccgatc tcaagatcga gatcgtcgag gtgccgtacg
     2701 aaaacggcag cctgcccggc tatttcgtgc acgccgagaa cgcgaaggtc gggcgcgtgc
     2761 cctgcgtcgt gttcttcgac ggtctcgatg tcaccaagga aatccagttc gtccgcggcg
     2821 tgcccgacct cgtcaagcgc ggcatcgcct gcctggtcat ggacgccccc ggcaccggcg
     2881 aggcgatccg cttccgcaat tattacctgc gccacgacta cgaggtcgcg ggcagcgcct
     2941 gcgtcgactg gctggagaag cgcgccgagg tggacccgaa gaaaatcggc atcgtggcga
     3001 tcagcctcgg cggctactac gccccgcgct gcgccgcgat ggagccgcgt ttcgccgcct
     3061 gcatcgcctg gggcgcgcag tgggactacc acgccacctg gaaaaagcgc atcgacgcgg
     3121 cctacaagac ctcgctctcg gtgcccgggc accacatcga gtggatcatg ggagcgaaat
     3181 ccgtggacga ggcgctcgcg aagctcgagc cgttcaagct cgacggcgtg atgcagaaaa
     3241 tgcgctgccc gttcctgctg gtgcacgggg cggacgacga gcagatccct ctcgccgacg
     3301 cgcaaaagca gttcgacgcc tgcggctcga gggacaagac cttccgcgtc ttcaccgcgg
     3361 aggaaggcgg cgcgcagcac tgccagcgcg actacctcac gctggtggtg gcggagatgt
     3421 ggaactggtt cgaagataag ctgctgcgtg ataagtgacg ggtgataagt gataagtcat
     3481 ccgcgctcgc gcggaacgtc aacttcctat cacttatcac ttatcaccta tcacccgaga
     3541 tgggaggagc catgatcgag agaatcgaaa gtttcggcgg gatcctggtc aagggctttc
     3601 acctgctcgc actgttcgtg atcgggggta ccgtggtctg gtccgcggcc catttctaca
     3661 tcgaaaccat gcagcagggc tatgccaggc tgcatgacat cctgctgcta ttcatctacc
     3721 tggagatcgg ggccatggtt gggatttatt tcaagaccca ccgcctgccg gtgcgcttcc
     3781 tgatctatgt cgcgatcacg gcgctgaccc gcctgctcac catcgatgcc aaggacctgt
     3841 ccaacgagca aatccttacc gtgaccggcg cgatcctggt gctcacgctg gcggcgctgg
     3901 ccgtggagtt cagcgcggcg cgcttccagt cgcctgaaca ggacgtctag cccatgggaa
     3961 ccgtcgaaag ccgccttgcg gcgctcgggc tgacgctgcc cgatgcgcca tcgccggcgg
     4021 cgaattacct tccgtacacg atggcgaagg gcctgatctt catagccggg caggcgccgg
     4081 tcattgatgg aaaataccag agcgtaggcc gggtcggcgc cgaagtgacg ctggaggccg
     4141 cgcaggcggc ggcccggctg tgcgggttga acgtgctcgc acaagtcaaa gccgccgtgg
     4201 tcggcgactg gtctcgggtc aggagttgcg tgcggctatg cggctacgtg agctccgcgc
     4261 ccggcttcca tgatcagccc agggtcctcg acggagcatc cgacctgatg gtgcaggctc
     4321 tgggcgatgc cggcaagcac gcccgctccg cgctgggcgt cttctccctg cgcggcaacg
     4381 tgccggtggt gatcgacgcg atcttcgagc tggcggatta gaacccgtct caaagatagc
     4441 cacgaaatca cacggaatga cacggacaag aacccggccc ggttaatgtc cgcgtgacca
     4501 gttaagaagt ctgtgtggtt ccgtgtgctt ccgtggctgt aacggctttt gaggtggctt
     4561 ttagggtttc ttgccgcgca acagcttggc gctgcgcttt accgctttca cgatattgat
     4621 gtagccggtg cagcggcaga ggttgccctc gagccccttg tagatgtcgt cgtcggtggg
     4681 attgggcttg tctttcagga gccccgccgt cgccatgatc atgcccggcg tgcagaagcc
     4741 gcattgcagg ccgtggcatt ccgagaacgc ctgctgaatc gggtgcagct tgcccccctt
     4801 cgccatgccc tcgatcgtca ccacggttgc gccgtttgcc tgggccgcga gcagggtgca
     4861 ggacttcacc gccctgccgt tcacgtggac ggtacaggcg ccgcactggc tcgtgtcgca
     4921 gcccacgtgc gtgccggtga gcttgagctc atcgcgcagg aagttgacca gcagcatgcg
     4981 gtcctcgacc gacttcgtat agttcttgcc gttgaccttg atggtgactt tagccatgtt
     5041 cttttcccga aagtgattcg tgtatcgtga ttcgtgatcc gagtcttacc tgtcttccga
     5101 tcggcgttta tcggcgttca tcggcggcta tttcttgcct gacagcttcg ccagtgcccg
     5161 cttcgccatc acccgcacca ggttggcgcg gtagtcgcgg tcggcgtgga tatcgctgtt
     5221 gaactcgcgg gagtcgagct tcacgccctc cagcgcggca ggcttcaggc ccttcgccag
     5281 cgcggcttcc gcttctttcc agcggaatac gccgggaccg gcgccggtca ccgcgaccct
     5341 cacgccctcg gccgtctgcg cgatgaagac gccggccatc gcgtagcccg atgccggatg
     5401 gtggaacttg gagtaggccg cgcgcttcgg cagcggaaat tcgacgcgca cgacgatttc
     5461 accctcctcc agcgcggtcg tgaagagtcc ctggaaatac tggtccgcgg cgatctggcg
     5521 cttggtcgtg accacggtcg cattgagccc gagcaccgcg gcgggataat ccgcggcggg
     5581 atcgttgttc gccaccgagc cgccgatcgt gccgcggttg cgcacctggg gatcgccgat
     5641 cgcggaggcg aggcccgcga gcgccgggat cgccttcttc acgacggccg atgtcgcgac
     5701 ctcatgatgc ttcgcccccg cgccgatcac gagcctgccg cccttcgcct cgattctgcg
     5761 tagctcggca agatccccga tgtccaccag gtgactgggc cgcgccagcc gctgcttcag
     5821 ggtcggcagc agcgtcatgc cgcccgacag caatttcgct tccgggtggg aggcgaggaa
     5881 cgccgccgcc tccttcaccg attttgcctt gacgtaattc agtgcgtaca tcgttacccc
     5941 tttctcacag cgttgatggc atgccagacg cgggaaggcg tcagcggcat atcgaggtgc
     6001 tggattccca gcgggcgcag cgcgtccatc acggcgttgg caagcgcggg cagcgagccc
     6061 gtgcagccgg attcgcccac gcctttgacc ccgagcgggc tcaccttgct cggcgtcggg
     6121 tgctcttcgc cgcggatcgc gggcaccagc cccgcgcgcg gcatgaagta atccatgaag
     6181 ctccccgtca ggagctggcc ggtgccgcgg tcgtagacta cctgctcgcc gaagatctgg
     6241 cccgcgccca tcgcgacgcc gccgtggagc tggccctcga cgacctcgtg gttgatcacc
     6301 gtgccgacat cgtcca
//
LOCUS       DBNGSG010000202         4039 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_65924, whole genome shotgun sequence.
ACCESSION   DBNGSG010000202 DBNGSG010000000
VERSION     DBNGSG010000202.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_65924"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="AC158Q_05410"
     CDS             complement(<1..191)
                     /locus_tag="AC158Q_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339244.1"
                     /translation="MLRAGFGWPDFPLRRAYRFFLILLAVLAVRFALSANRIARQPSW
                     PVTIPNIGSTHAIASSTCR"
     gene            complement(436..1395)
                     /locus_tag="AC158Q_05415"
     CDS             complement(436..1395)
                     /locus_tag="AC158Q_05415"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3339245.1"
                     /translation="MKSIMHFALAFAFLLPGSAMAQQYPAKPVRIVSPFPAGGSVDLV
                     ARMVAAKLTEVVGQQVIVENRSGASGNIGTEVVMRAAPDGYTLLVHTIPFVANSFLSS
                     KLPYDPLNDFAPVMLVSSSPSLLAVHPSLPVRSVKELIALARSRPGELNYGTAGPATN
                     PHIVGEFLNLMGKTHIVAVHFKGGGPAIIAAISGEVGIAFTNFSETSAFVKSGRLRGL
                     GVSSPKRVAVMPDLPAIAETLPGFEFTTWQGILAPAKTPRPIIAALNERLKKAMAAPD
                     QMKRFQDRGLDVIASSPEEFSAHVQSEYRKWGKVIKERGMKAE"
     gene            complement(1470..1769)
                     /locus_tag="AC158Q_05420"
     CDS             complement(1470..1769)
                     /locus_tag="AC158Q_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HIR3339246.1"
                     /translation="MAAKLLESIPPGEILYEDFMKPMGVSINGLAREIAVPPNRISDI
                     VNGKRSITADTVLRLGKCFGVLPELWLNLQVECDLRVAKRTTWLKAEPRVRAHSD"
     gene            complement(1799..2143)
                     /locus_tag="AC158Q_05425"
     CDS             complement(1799..2143)
                     /locus_tag="AC158Q_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3339247.1"
                     /translation="MSKHKASDRVAAYAGGGNVYADLGYPDADEMLVKAQLVSKIAEI
                     IRRKGLTQVEAAKRLGLTQPKISAMLRGQFRGFSERKLIDCLTSLGRDVQIVIKESPE
                     RRAGGKLTVVIA"
     gene            complement(2121..2495)
                     /locus_tag="AC158Q_05430"
     CDS             complement(2121..2495)
                     /locus_tag="AC158Q_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIR3339248.1"
                     /translation="MPVKLLEWVGSSKKDLKALPDEVIDIFGYALFLAQTGGKHGQAK
                     PLQGFGSAGVLGVVEDYRGDAYRAVYTVRFEERVFVLHVFQKKSKTGIATPKKDMDLI
                     RERLKVATRRAKELKNEQAQGK"
     gene            2950..3753
                     /locus_tag="AC158Q_05435"
     CDS             2950..3753
                     /locus_tag="AC158Q_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR3339249.1"
                     /translation="MKSVPRKSTVTRIRPNRNPLGVSAAEWDARCELAAAFRLAHSLG
                     WSDFLGTHFSLRVPGTGDQFLINPYGLLFDEITASSLLKVDTEGNVLTESPYEMNRAG
                     FVIHSAVHMGAKDAHCVMHTHTQAGVGVASQKQGLLPITQMALTIWGELRYHDYEGVA
                     DNEDERSRIVKDLGDGTMVILRNHGTLTVGRNVGEAFSRMNRIERACKFQLAALTGGA
                     EPNLIPNEVVEYTAQQGREINNEGRAAGGKLLWAALKRRLDRDDPGYRQ"
BASE COUNT          764 a         1326 c         1198 g          751 t
ORIGIN      
        1 aagcggcagg tgctggaagc gatcgcgtgc gtgctgccga tgttcggaat ggtaactggc
       61 cagctgggct ggcgtgcgat gcggttggcg gacaaagcaa aacgaaccgc caggaccgcc
      121 aagagaatca gaaaaaagcg ataggcgcgc cgcaacggga aatcaggcca cccgaatccg
      181 gcccgcagca tcgggccgtg cttcgctggc tctaacgtgc gcgccaagaa aaggttcctg
      241 acaccttttc tgcccccaag cagcgctcac cactcgccgc agctacgacg agtgcgttgc
      301 tttggccagg agagcattcg ggtaagtcac cgcaccgggg ctgcaccccc gaaaatcgga
      361 aggagggcct tgggttgttc ttccctattc ctgtttcctc tgtgtccttc gtgtcctctg
      421 tgtctcagct ttacgtcact cggctttcat gccgcgctcc ttgatcacct tcccccactt
      481 cctgtactcg ctctgcacgt gcgcggagaa ctcctccggt gagctcgcga tcacgtcgag
      541 cccgcggtcc tggaaccgct tcatctggtc cggcgcggcc atcgccttct tgagccgctc
      601 gttcagggcc gcgatgatcg gccgcggcgt tttcgccggc gccagtatcc cctgccaggt
      661 cgtgaactcg aatcccggca gcgtttcggc gatcgcgggc aggtccggca tcaccgccac
      721 gcgcttcggg ctgctcacgc cgagcccgcg cagacgcccg gacttcacga aagccgaggt
      781 ctcggagaaa ttcgtgaacg caatgccgac ctcgccgctg atcgccgcga tgatcgcggg
      841 gccgccgccc ttgaagtgca ccgcgacgat gtgggtcttg cccatcaggt tgaggaattc
      901 cccgacgatg tgggggttgg tggcggggcc ggcggtgccg tagttgagct cccctggccg
      961 cgaacgcgcc agcgcgatca gctccttcac cgagcgcacc ggcagggagg ggtgcaccgc
     1021 cagcagcgac ggcgaggagg aaaccagcat gaccggcgcg aaatcgttca acggatcgta
     1081 cggcagcttg ctggagagga agctgttggc gacgaacggg atggtgtgta ccagcagcgt
     1141 gtagccgtcc ggcgccgcgc gcatcaccac ctcggtgccg atgttgccgg aggcgccgct
     1201 gcggttctcg acgatgacct gctgccccac gacctcggtc agcttcgcgg ccaccatgcg
     1261 tgcgacgagg tccacgctgc cgccggcggg aaacggcgag acgatccgca ccggcttcgc
     1321 gggatactgc tgcgccatcg cggatcccgg gagcaggaat gcgaatgcca gggcaaagtg
     1381 catgatgctt ttcaaggtct cctccttcta tatccgttac tcgttattag ctattagtta
     1441 ttcattactt gttacgcgtc acccggcctt cagtccgaat gggccctgac ccgcggctcc
     1501 gccttcaacc aggtcgtccg cttggcgaca cgcaggtcgc actcaacctg gaggttgagc
     1561 cacaactccg gcaagacccc gaaacacttg ccgaggcgca gcaccgtgtc ggcggtgatc
     1621 gagcgcttgc cattgacgat atcgctgatg cggttgggcg gcaccgcgat ctcgcgcgcg
     1681 agcccattga tgctcacgcc catcggcttc atgaagtcct cgtagagaat ttcgccggga
     1741 ggaatcgatt cgagcagttt ggcggccatg gcgtctccca ggaatggtaa tcgacgagtc
     1801 aggcgatcac gaccgtcagc ttgccgcccg ccctgcgctc gggcgattcc ttgatcacaa
     1861 tctgcacgtc acggccgaga ctggtcaggc aatcgatcag ctttcgctcg gagaatcccc
     1921 ggaactggcc gcgcagcatc gccgaaatct tgggctgggt gagcccgagg cgcttcgccg
     1981 cctcgacctg ggtaagtccc ttgcgccgga taatctccgc gatcttggag acgagctggg
     2041 ccttgaccag catttcgtcc gcatccggat agcccagatc ggcgtaaaca ttaccgccgc
     2101 cggcgtaggc cgctacgcgg tcacttgcct tgtgcttgct catttttcag ctccttggcg
     2161 cgccgggtcg ccactttcag tctttctcga atcagatcca tgtctttctt cggggtggca
     2221 ataccggttt tcgatttctt ctggaacacg tgcagcacga acactctttc ttcgaatcga
     2281 accgtataca cggcgcgata ggcgtctccg cgataatcct cgacgacccc cagtactccc
     2341 gcggaaccga atccctgcaa aggtttcgcc tggccatgct tacccccggt ttgcgccagg
     2401 aagagcgcat aaccgaagat atcgatcacc tcatccggca gcgccttgag gtcctttttc
     2461 gagctcccga cccattcaag cagcttgacc ggcatcggcc ctctccatgc cggattatac
     2521 ccgaattggg atacttcaaa aaagaagcga attcaccatc gaaccccagc acaccgtaag
     2581 tcaagtttga gtcaagttat cagtcaagac gggcggcgcc ccatggggac agaacaaggt
     2641 ttgctgacac cctttttccc gttcagcagt gccccgcgtg ttgagcggta gaaattcgtc
     2701 cgttcgaaca cgcggagcta aaccggcgcg cagcgcccgg atttaacgat acttggcgcg
     2761 tttccttcgc gacccgtcgt aatctttcct tcgtgtcctt tgtgtccttc gtggcaaagc
     2821 tttacgtcga tcggcccttc gcctcgccct cccccaatcc ctccgccctc agccctgcct
     2881 ttatgggaaa atgcctgcac ccgccaaccc ggcccgttcc acatagtggc gccccctcca
     2941 ggagacagca tgaaaagcgt tcccaggaaa tccacggtca cccgcatccg ccccaaccgc
     3001 aatcccctcg gcgtgagcgc cgcggaatgg gatgcccgct gcgagctcgc ggcggcgttc
     3061 cgcctcgcgc acagcctggg atggagcgac ttcctgggga cccatttctc gctgcgcgtg
     3121 ccgggcaccg gggaccagtt cctgatcaat ccctacggcc tgctgttcga cgagatcacc
     3181 gcgtcctcgc tgctcaaggt ggacacggaa ggcaatgtgc tgaccgagtc cccgtacgag
     3241 atgaaccggg cggggttcgt catccacagc gccgtccaca tgggcgcgaa ggacgcgcac
     3301 tgcgtcatgc acacccacac gcaggccggc gtcggcgtcg cttcccagaa gcagggcctg
     3361 ctgccgatca cccagatggc gctgacgatc tggggcgagc tcaggtacca cgactacgaa
     3421 ggcgtggccg acaacgagga cgagcgcagc cgcatcgtca aggacctcgg cgacggcacc
     3481 atggtgatcc tgcgcaacca cggcacgctc acggtgggcc gcaacgtcgg cgaagcgttc
     3541 tcgcgcatga accgcatcga gcgcgcgtgc aagttccagc tggccgcgct caccggcggc
     3601 gccgagccca acctgatccc caacgaagtc gtggagtaca ccgcgcagca gggccgcgag
     3661 atcaacaacg aaggccgcgc cgcgggcggc aagctgctgt gggcggcgct gaagcggagg
     3721 ctcgaccggg acgatcccgg ctatcgccag taggagcaag tcaaaagtcg gaagtcgaaa
     3781 gtcgaaagtg tcaaaaccgg aacctctgta aagcaagtcg aaaggcgaaa gtcggaagta
     3841 tgaaaaccgg tgcttcgccg gaaccctgat gagtaagacg aagttcaaaa ggcggcagta
     3901 agaacagccc gtcggagacg ggccacaatt ttcgcctttc gactttcgcc tttcgacttc
     3961 agtcagccgc gctcgccggc gatggtcacg ccgccgtcgg cgacgatcat ctgcccggtg
     4021 acgaaggagc ccgcctcgg
//
LOCUS       DBNGSG010000203         3916 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1085322, whole genome shotgun sequence.
ACCESSION   DBNGSG010000203 DBNGSG010000000
VERSION     DBNGSG010000203.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1085322"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="AC158Q_05440"
     CDS             <1..242
                     /locus_tag="AC158Q_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HIR3339250.1"
                     /translation="PPGLAKKSNGCMPPGQAKKWQIGRPLPREVIFHDLPRSILVELG
                     PPPAGHRFVRVAADILLIAIGTGMVIDAIEDLGRM"
     gene            complement(308..1300)
                     /locus_tag="AC158Q_05445"
     CDS             complement(308..1300)
                     /locus_tag="AC158Q_05445"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3339251.1"
                     /translation="MKMTTSQAVVLLAASVALVPSGSAWGQPYPSRPVRLIIGGLPGT
                     APDIIARIVGPSISESMGQSLILDNRGGGAGLLGAQITAAAAPDGYTFLIVGGGSLVI
                     VPYLTKNPPYDPVGDFTPVTLVSLAPLVLASNLALPVKSVQELISLSKAKPKDLLYGT
                     PGVGSIHHLTVEMLNRAAGINLQHVPYKGGPPAVVDAIAGRVQLVITTVIPLSPHIKA
                     ARLRGLAVTSAKRTSVFPDLPTVAESGVKGFESHQWFGIFTPKNTPATVRERFYKEVR
                     KAVDKPGATAILDQEGQLLAVNGPKALDEFRRTETAKWTKIISQLQAAGAIELR"
     gene            1529..2221
                     /locus_tag="AC158Q_05450"
     CDS             1529..2221
                     /locus_tag="AC158Q_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3339252.1"
                     /translation="MKFERALIVGAGQGLSAALARLFSKEGMKIALASRSPDGLAALC
                     AETGARAFACDAVQAGQVRQLFADVDKAVGEPDVVVYNASGRVRGPLVDLDPAEVERA
                     LAVSAFGGFLVAQQAARRMLAKRRGAILFTGASASVKGYPRSASFAMGKFALRGLAQS
                     MARELAPQGIHVAHFVIDGGIARPGRAAPSAEPDALLDPDAIAASYLSVLRQPRSAWT
                     WEVELRPWVEKF"
     gene            complement(2441..3154)
                     /locus_tag="AC158Q_05455"
     CDS             complement(2441..3154)
                     /locus_tag="AC158Q_05455"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014626.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HIR3339253.1"
                     /translation="MCGRKYNPETAAMEREWHIGRHNNNPFRNLYDARPTQFLPILRM
                     SPEHGIELDAFRWGLVPSWWKKPLKEVGGTFIATVEKIAANSGMWRTPFLRRRCLVPV
                     HGFYEWQYQPDGTSVRHYIKLRDADLFTLAGLWDMWKQPDGTLLGSYAVITCPANPLM
                     ARIHNRPKKSEGPRMPVILTGETAKAWFDPGVELSPEERLGLLQPYSQEAMVAYPVVN
                     TAADPADLIKPIGEPLALL"
     gene            3399..3620
                     /locus_tag="AC158Q_05460"
     CDS             3399..3620
                     /locus_tag="AC158Q_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HIR3339254.1"
                     /translation="METIEFQLKGEHVRLCDLLKLAGIAESGGQGKRLVAAGEVNVDG
                     QPESRKAARIRAGQTVECHGAKIVVVPAA"
BASE COUNT          732 a         1170 c         1318 g          696 t
ORIGIN      
        1 gccccccggg ccttgccaag aagagcaatg gttgcatgcc gcccggccag gcgaagaagt
       61 ggcagatcgg ccggccgctg ccgcgggagg tgatattcca tgacctgccc cggtcgattc
      121 ttgtggagct cggaccgccg ccggctgggc atcgtttcgt ccgcgtggcc gccgacatcc
      181 tgctgatcgc catcggcacc ggaatggtca tcgacgcaat cgaggatctg ggccggatgt
      241 aacaggaata atcagaataa ttagggccgg gtccacgttt ctgaaaagtg aacccggccc
      301 cggccaacta tctgagctca atggcgccgg ccgcctgcag ctgggagatg atcttcgtcc
      361 actttgccgt ctccgtccgg cgaaactcgt caagcgcctt cggtccattg accgcgagca
      421 gctggccttc ctggtcgagg atcgctgtcg cgccgggctt atccaccgcc ttgcgtactt
      481 ccttgtagaa cctttccctg acggttgccg gcgtgttctt gggcgtgaag atcccgaacc
      541 actggtgaga ctcgaatccc ttcacgcccg actccgcgac ggtcgggaga tcgggaaaca
      601 cgctcgtgcg ctttgcgctg gtcaccgcaa gcccccgcag gcgtgcggcc ttgatgtgag
      661 gcgaaagcgg gatgacggtg gtaatgacga gctgcacgcg gccggcaatc gcgtccacca
      721 ccgccggcgg cccgcccttg tagggaacgt gctgcaggtt gatgccggcg gcgcggttca
      781 gcatctcgac cgtgagatgg tggatcgaac cgacgccggg ggtgccgtaa agcaggtcct
      841 tgggctttgc tttggagagg ctgatcagct cctggaccga tttgacgggc agcgcaagat
      901 tgctcgccag cacgagcggc gcaagcgaga cgagcgttac gggcgtgaaa tccccgaccg
      961 ggtcgtaggg aggatttttc gtgagatagg gaacgatcac gaggctgccg ccgccgacga
     1021 tgagaaaagt gtagccgtcg ggagcggcgg cggcggtgat ctgcgctccg aggaggccgg
     1081 ccccgccccc gcgattgtcc aggatgaggg actgccccat tgactccgag atgcttgggc
     1141 caacgatgcg cgcgatgatg tccggcgccg tgcccggcaa gcccccgata atgagcctga
     1201 cgggcctgga cggataaggc tgaccccatg ccgacccgct cgggacgagc gcgaccgatg
     1261 cggcgagaag tacgactgct tgagacgttg tcattttcat acgcggctcc tcctcctgat
     1321 tcaatctgca ttcaacattt cgtatgcatg ccaatcgatc tagatgccgt gagtaagggt
     1381 caaactgccc tttccgggaa gtatcgtgca aaaccccaac caatgccata gccgcagttt
     1441 tcggggtggg ggcacatccc cgaacaggtt cggggccccg gtccagcccc tcgacgcgcg
     1501 acctcgtcac tcctattaaa ctagggccat gaagttcgag cgtgcgctga tcgtcggggc
     1561 ggggcagggg ctgagcgctg cgctcgcccg gctgttctcg aaagaaggca tgaagatcgc
     1621 cctggcctcg cgtagccccg acgggctcgc ggcgctgtgc gcggagaccg gcgcgcgcgc
     1681 gtttgcctgc gacgccgtgc aggccggcca ggtgcgacag ctgtttgccg acgtggacaa
     1741 ggcggtcggc gagcccgatg tcgtggtgta caacgcgagc ggacgggtgc gggggccgct
     1801 ggtcgatctg gacccggccg aagtcgagcg ggcgctcgcc gtgagcgcct tcggcggttt
     1861 cctcgtcgcg cagcaggcgg cgcgccgcat gctcgcgaag cggcgggggg cgatcctctt
     1921 caccggagcc tcggcgagcg tcaagggcta tccccggtcg gcgtctttcg cgatgggcaa
     1981 gttcgccctg cgcggcctcg cccagagcat ggcgcgcgag ctcgcgccgc aaggcataca
     2041 cgtcgcccat ttcgtgatcg acggcggcat cgcccgcccc gggcgcgctg cgccgtcggc
     2101 ggagcccgat gcgctgctcg acccggatgc tatcgcggcg agctacctga gcgtgctgcg
     2161 ccagccgcgc agcgcgtgga cgtgggaagt ggaattgcgg ccttgggtgg agaagttctg
     2221 acgtaaagcc tcaccgccaa gcatgtcctg agtaagtcga agggcctgtt ctgagtgagt
     2281 cgaaggggac gccaaggccg ccaaggtaac cgcactaaag cttagacacg gaggacacgg
     2341 aggacacaga ggaaataacc tatagagaag tccttgcgct ccggatcatg gatcacgcat
     2401 tctcggggga aagggcgcga aggaaatgcg caaaggtggc ctacaggagg gcgaggggct
     2461 cgccaatggg cttgatgaga tcggctggat ccgcggccgt gttcactacc gggtaggcaa
     2521 ccatcgcctc ctgggaatat ggctgcaaca gccccagccg ttcctcaggc gacaattcca
     2581 cgcccgggtc aaaccaggct ttcgcggttt cgcccgtcag gatgaccggc atgcgcggcc
     2641 cctcgctttt cttgggcctg ttgtgaatcc gcgccatcag cgggtttgcc ggacaagtga
     2701 tgaccgcgta cgaacccagc agggtgccgt cgggctgctt ccacatgtcc cataggccgg
     2761 cgagcgtgaa caggtcggca tcgcgcagct tgatgtagtg ccgcacggac gttccgtccg
     2821 gctggtactg ccactcgtag aacccgtgga cgggcacgag gcagcgccgg cgcaggaacg
     2881 gcgttcgcca catgccgcta tttgccgcga ttttctcgac ggtggcgatg aaagtgccgc
     2941 ccacttcttt cagcggcttc ttccaccagg agggcaccag gccccagcgg aacgcatcga
     3001 gttcgatgcc gtgctcgggg ctcatccgca ggatgggaag gaactgcgtg gggcgggcat
     3061 cgtagaggtt gcggaaaggg ttgttgttgt gcctgccgat gtgccactcg cgctccatgg
     3121 ccgctgtctc ggggttgtat tttcgcccgc acatctggaa agtataaggc ggaagtgctt
     3181 gccgtttgac actgcgtctc aggtaacacg tctcaagtaa tacgtctcag gtactgcgtt
     3241 tcaggtaatg cgtctcaggt aacacgtctc aggtacggcg cgatttatct ggaatgcttt
     3301 accttcgacg cactccaggc cggggcggag cgtaagcttg gcccctttgg ctacttccgg
     3361 aagaccgcct cttcgcggat actcgccgca ggagggttat ggagacaatc gagttccagt
     3421 tgaaaggcga gcacgtcagg ctgtgcgacc tgctcaaact cgcaggcatt gccgagagcg
     3481 gcggtcaggg gaagcgactg gtcgctgccg gcgaggtcaa cgtggacgga cagccggaga
     3541 gccgcaaggc ggccaggatc cgcgccgggc agaccgtgga atgtcacggc gcgaagatcg
     3601 tcgttgtgcc agccgcgtaa ataggaaggc gaaagtcgaa agtgtaattt ccgcagcgcg
     3661 gttagctaaa gcttcaccgc caagcgtgtc ctgagtaagt cgaagggcct gtcctgagtg
     3721 agtcggaggg gacgccaagg ccgccaagga acgattacga cgggtcgcta aggacgtgga
     3781 ggacgcgaag gaaagaccta ctacgggtca cgaaggacgc aaaggccgcg aaggaaacag
     3841 atatcggaaa cccaaggccg cgaggacaac gccaagtctg gcatacagga agcgctctcg
     3901 gggttatccg tgccag
//
LOCUS       DBNGSG010000204         4716 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2443011, whole genome shotgun sequence.
ACCESSION   DBNGSG010000204 DBNGSG010000000
VERSION     DBNGSG010000204.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4716)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4716)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4716
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2443011"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..108)
                     /locus_tag="AC158Q_05465"
     CDS             complement(<1..108)
                     /locus_tag="AC158Q_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR3339255.1"
                     /translation="MRHIISVLLENESGALSRVAGLFSARGYNIESLTVA"
     gene            complement(105..1802)
                     /locus_tag="AC158Q_05470"
     CDS             complement(105..1802)
                     /locus_tag="AC158Q_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 catalytic subunit"
                     /protein_id="HIR3339256.1"
                     /translation="MELTGAEIVVRCLQEEGVEHVFGYPGGAVLFIYDELFKQNKVKH
                     ILVRHEQGAVHAADGYARSTDKVGVSLVTSGPGVTNAVTGIATAYMDSIPLVIITGQV
                     PTHAIGLDAFQECDTVGITRPCVKHNFLVKDVKDIAVTMKKAFYIATTGRPGPVVVDI
                     PKDVTTAKAEFRYPETATMRSYNPVTKGHAGQIKKAIQTLMQAKRPMIYAGGGVILGD
                     ASQELAQLTRALGFPCTNTLMGLGAFPATDRQFVGMLGMHGTYEANMAMQHCDVLLAI
                     GARFDDRVIGNPKHFFNEERTIIHVDIDPSSISKRVKVDIPIVGTVREVLKDLIKYLD
                     AAKEKPDREALKTWWTQIELWRSRDCLKYDRAAAIIKPQYVIEKLYEVTQGDAFVTSD
                     VGQHQMWAAQFYKFEKPRRWINSGGLGTMGFGLPAAMGVQLAHPGAMVACVTGEASVQ
                     MCIQELSTCKQYRLPIKVVNLNNKYMGMVRQWQEFFHGNRYAESYMDALPDFVKLAES
                     YGHVGMKIEKPADVEPALKEAFARKDELVFMDFITDQTENVFPMVPGGKGLSEMILV"
     gene            2033..2599
                     /locus_tag="AC158Q_05475"
     CDS             2033..2599
                     /locus_tag="AC158Q_05475"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3339257.1"
                     /translation="MATQKELSDFFAEVERRAYKQALFAVRDDHIALDIVQDAMLKLT
                     EKYAVRPAAELPLLFQRILQNTIRDHYRRQKVRALWTTPVSSLLGAEADGEGDPLETL
                     EVEDDSKFSEPPAEQLERSQVLAFIEKALEKLPARQREAFLLRYWEELDVAEAAAIMG
                     CSEGSVKTHCSRATRALAITLRKQGIKL"
     gene            2596..2985
                     /locus_tag="AC158Q_05480"
     CDS             2596..2985
                     /locus_tag="AC158Q_05480"
                     /inference="COORDINATES: protein motif:HMM:NF023699.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3619 family protein"
                     /protein_id="HIR3339258.1"
                     /translation="MNRPEHELARTIVRHLDYGVDQLEAGTRERLLAARKAALSQYRE
                     RPAPVAGLAWAGQVVAYISEHRYTSVRHLIAISMLVAVLAGVAYWQNNGTTNELADID
                     TGLLTDELPINAYLDKGFDSWLKRSSR"
     gene            2961..3488
                     /locus_tag="AC158Q_05485"
     CDS             2961..3488
                     /locus_tag="AC158Q_05485"
                     /inference="COORDINATES: protein motif:HMM:NF022748.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HIR3339259.1"
                     /translation="MAQAFVALTVCACLCLAFPAVAAQTDKGAKKPVRPAWSELKPAH
                     QKILAPLAPEWDRMDATRRKKWVAIAERYPKMKPQEQQRLQTNMTAWAKLTPLQRKAA
                     REKYQTLKKLPPDQRRQVADQWKRYQQSLAAQPELSPSDPPAPPEPQIAETPAATPPQ
                     AEGTATAGVPAPAAQ"
     gene            3508..3942
                     /locus_tag="AC158Q_05490"
     CDS             3508..3942
                     /locus_tag="AC158Q_05490"
                     /inference="COORDINATES: protein motif:HMM:NF018028.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HIR3339260.1"
                     /translation="MRPPDAAGAQAAGLSRRALALVYETLLLAALLLAGALPFVMLAQ
                     GMDRGLARPLFQLYLVSLSGAYFIWQWRRGGRTLPMKTWRLRLVTRDGAALDWTHAAK
                     RYLIALPGALLLGAGFLWALVDREGLFLHDRLAGTKIVMSDE"
     gene            complement(4003..>4716)
                     /gene="lptG"
                     /locus_tag="AC158Q_05495"
     CDS             complement(4003..>4716)
                     /gene="lptG"
                     /locus_tag="AC158Q_05495"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HIR3339261.1"
                     /translation="VAPPAEQLAQRVRSQAITGLVAQEFRSGLWIKDGNSFINVVEVL
                     PDTTLRGVRIYEFDGDYRLRSISYAQRGAYQHDRRWLLQGMVQTRFENMRTSVHEIAE
                     TRWQSVLEPDLINVLLVKPEQMSAWNLYSYTQHLKENRQKALRYEIALWTKLTYPLAV
                     VVMMVLALPFAYFQGRQAGVGAKIFAGIMLGIVFHFLNRLFSHLGLLNDWPPAASALM
                     PTVTFLTIAVGMMWWQERR"
BASE COUNT          846 a         1524 c         1501 g          845 t
ORIGIN      
        1 cgcgaccgtc agcgactcga tgttgtagcc gcgcgccgag aacagccccg caacgcgcga
       61 cagcgccccg gactcgttct cgagcagcac ggagatgatg tgcctcatac caggatcatc
      121 tccgagagcc ccttgccgcc gggcaccatc ggaaacacgt tctcggtctg gtcggtgatg
      181 aaatccatga acacgagctc gtccttgcgc gcgaacgcct ccttcagcgc cggctcgacg
      241 tcggccggct tctcgatctt catgccgacg tgcccgtagg attcggcgag cttcacgaaa
      301 tccggcagcg cgtccatgta ggactccgcg tagcggttgc cgtggaagaa ctcctgccac
      361 tgccgcacca tgcccatgta cttgttgttc aggttcacga ccttgatcgg gagccggtac
      421 tgcttgcagg tggagagctc ctggatgcac atctgcacgc tcgcctcgcc ggtgacgcag
      481 gcgaccatcg ccccggggtg cgcgagctgc acgcccatcg ccgcggggag cccgaagccc
      541 atggtgccga gccccccgga gttgatccag cgccgcggct tctcgaactt gtagaactgc
      601 gccgcccaca tctggtgctg gccgacgtcg gaggtgacga acgcgtcgcc ctgcgtcacc
      661 tcgtagagct tctcgatcac gtactgcggc ttgatgatcg cggccgcgcg gtcgtatttg
      721 aggcagtcgc gcgagcgcca tagctcgatc tgggtccacc aggtcttcag cgcctcgcgg
      781 tcgggctttt ccttcgcggc gtcgaggtac tttatgaggt ccttcagcac ctcgcgcacg
      841 gttccgacga tcgggatgtc caccttgacg cgcttggaga tcgaggacgg gtcgatgtcg
      901 acgtggatga tggtgcgctc ctcgttgaag aagtgcttgg gattcccgat cacccggtcg
      961 tcgaagcgcg ccccgatggc gaggagcacg tcgcagtgct gcatcgccat gttggcctcg
     1021 taggtgccgt gcatgccgag catgccgacg aactggcggt cggtcgccgg gaaggcgccg
     1081 aggcccatca gggtgttcgt gcacggaaac cccaatgcgc gcgtcagctg ggccagttcc
     1141 tgcgaagcat cccccaggat gacgccgccg ccggcataga tcatcgggcg ctttgcctgc
     1201 atgagcgtct ggatcgcctt cttgatctgc ccggcgtgcc ccttggtgac cgggttgtag
     1261 gagcgcatgg tggccgtttc gggatagcga aactcggcct tggccgtggt cacgtccttg
     1321 ggaatatcca ccaccaccgg cccgggacgg ccggtcgtgg cgatgtagaa cgcttttttc
     1381 atcgtgaccg cgatgtcctt cacgtccttg accaggaagt tgtgcttcac gcagggacgc
     1441 gtgataccca ccgtgtcgca ttcctggaag gcgtcgaggc cgatggcgtg ggtcgggacc
     1501 tgtccggtga tgatgacgag cggtatggaa tccatgtaag cggtggcgat gccggtcacc
     1561 gcgttggtga cgccggggcc cgaggtgacc agcgagacac ccaccttgtc ggtggaacgc
     1621 gcgtagccgt cggcggcgtg caccgccccc tgctcgtgcc gcaccaggat atgcttgacc
     1681 ttgttttgct tgaacagctc gtcgtagatg aacagcaccg ccccgccggg gtagccgaac
     1741 acgtgttcga cgccctcttc ctgcaggcag cgcacgacga tttcagcgcc ggtcaattcc
     1801 atggcatggg tccaggcaat gcgaaaacgc tcaacgttac cggttgcggc cgctcaggtc
     1861 aagcaaaata tccctttaaa cagcaggata gagcaaccgg ccgcgaccgg ttataggtcg
     1921 tttgcgcgct gttggcgttt tgctaagatg ccccggaccc cggcgacggg cgcggagggg
     1981 cgcgtcccac gggctgcgaa ccgcagccgc cagttcaacc gaggcgagtc cactggccac
     2041 tcaaaaagaa ctgtcggatt tcttcgcaga agtcgagcgc cgcgcctaca agcaagcatt
     2101 gttcgcggtg cgggacgatc acatcgcgtt ggacatcgtg caggacgcca tgctcaagct
     2161 cacggaaaaa tacgccgtcc gccccgcagc cgagctgccc ttgctgttcc agcgcatcct
     2221 gcagaacacc atacgggatc actaccggcg gcagaaggtg cgcgcgctat ggacgacgcc
     2281 cgtatcgagc ctgctcggcg ccgaggccga cggtgaaggc gacccgctgg aaactttgga
     2341 agtggaggac gactccaaat tctcggagcc cccggcggaa cagttggagc gctcgcaagt
     2401 tcttgctttt attgaaaaag cacttgaaaa gctgccagcg cgtcaacgcg aggccttcct
     2461 gttgcgttac tgggaggaac tggacgtcgc agaggcagca gcgataatgg gttgctcgga
     2521 gggaagtgtg aaaacacact gctcgcgggc aactcgcgcc cttgcgatta ccctcaggaa
     2581 acaggggatc aagctatgaa ccggccggaa cacgaactgg cgagaaccat cgtccggcac
     2641 ctcgattacg gcgtggacca actcgaggct ggcacgcgcg agcgcctgct tgccgcgcgc
     2701 aaggccgcct tgtcgcagta ccgcgagcgg cccgcgccgg tggccggcct ggcatgggcc
     2761 gggcaggtgg tggcgtatat ctccgagcac cgctacacca gtgtccgcca tctgatcgcg
     2821 atttcgatgc tcgtggccgt gctggcgggc gtggcctact ggcagaacaa cggcaccacg
     2881 aacgagctcg ccgatatcga cacggggttg ctcaccgacg aactgccgat caacgcttac
     2941 ctcgacaagg gcttcgactc atggctcaag cgttcgtcgc gctgaccgtc tgcgcctgcc
     3001 tatgtctcgc gtttccggca gtcgcggcgc aaaccgataa gggcgccaag aaaccggtcc
     3061 ggcccgcctg gtcggagctg aagcccgcgc accagaagat ccttgcgccg ctcgccccgg
     3121 agtgggaccg catggatgcc acccggcgca agaaatgggt cgccattgcc gagcgctatc
     3181 ccaagatgaa gccgcaggag cagcaacgcc tgcagacaaa catgaccgcg tgggccaagc
     3241 tgacgccgct gcagcgcaag gcggcgcgcg aaaaatatca gacgctgaaa aaactgccgc
     3301 ccgaccagcg ccggcaggtg gccgaccaat ggaagcgata ccagcagtcg ctcgccgcgc
     3361 agcccgagct gtccccgtcc gatcccccgg cgccgccgga gccgcaaatc gcggaaacgc
     3421 ccgcggcaac accgccccag gccgagggca ccgcgaccgc aggggtgccg gcgcccgctg
     3481 cgcagtagcc cgggtttcct ggatacgatg cggcctcccg atgcagccgg ggcgcaggcc
     3541 gcgggccttt cgcgccgtgc attggcgctc gtttacgaga cgctgctgct cgccgcgctg
     3601 ctcctggccg gcgcgttgcc cttcgtgatg ctggcgcaag ggatggatcg ggggcttgcc
     3661 cgtcccttgt ttcagctcta cctcgtctcg ttgagtggcg cttatttcat ctggcagtgg
     3721 cggcgcggcg gccggacgct cccgatgaaa acctggcgcc tgcgcctcgt cacgcgcgac
     3781 ggcgcggcac tcgactggac acacgccgcg aagcgctacc tcatcgccct gcctggcgcc
     3841 ctgcttctgg gcgcgggctt tctatgggcg ctggtcgatc gcgaagggtt gttcctgcac
     3901 gaccggctgg cgggcactaa gatagtgatg agtgatgagt gatgagtgca aaatggttct
     3961 aaaactttgc actctgcgct ttacgtttca tattcggcgc ggctagcgcc gctcctgcca
     4021 ccacatcatt cccacggcaa tagtaaggaa cgtcacggta ggcatgagcg cgcttgccgc
     4081 gggcggccag tcgttcaaca ggcccaggtg cgagaacagc cggttgagga aatggaatac
     4141 gatccccagc atgatgcccg cgaagatctt ggcacccacc cccgcctggc gcccctggaa
     4201 gtacgcgaac ggcagcgcca gcaccatcat cacgaccacc gccagcggat aagtcagctt
     4261 ggtccacagc gcgatctcgt agcgcagcgc cttctggcgg ttttccttga gatgctgggt
     4321 gtaggaataa agattccacg ccgacatctg ttcgggctta accagcagca cgttgatgag
     4381 atccggctcg agtaccgatt gccatctcgt ctcggcgatc tcgtgcaccg aggtgcgcat
     4441 gttctcgaag cgggtctgga ccataccctg cagcagccag cgccggtcgt gctggtaggc
     4501 gccgcgctgg gcgtagctga tcgagcgcag ccggtaatcg ccgtcgaact cgtagattct
     4561 cacaccgcgc agcgtggtat ccggcagcac ttcgaccacg ttgatgaagc tgttgccgtc
     4621 cttgatccac aggccggagc ggaactcctg cgccaccagg ccggtgatgg cctgggagcg
     4681 cacacgctgc gcgagctgct ccgcgggtgg cgcaac
//
LOCUS       DBNGSG010000205         3239 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1594599, whole genome shotgun sequence.
ACCESSION   DBNGSG010000205 DBNGSG010000000
VERSION     DBNGSG010000205.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1594599"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(344..1171)
                     /gene="pbpG"
                     /locus_tag="AC158Q_05500"
     CDS             complement(344..1171)
                     /gene="pbpG"
                     /locus_tag="AC158Q_05500"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF008668.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HIR3339262.1"
                     /translation="MTLAPAMPPQARAAQPAGVAKSGVLDLKSSTALVVEQGGKQVLY
                     AKNVDAVVPIASITKLMTALVVVESGLPLEERIAITSEDRDGIKGTRSRLSIGTELTR
                     ADLLKLALMASENRAAAALTRAYPGGTSAFVAAMNQKAVRLGMWHTRFVDGTGLSSAN
                     VSTARDLVALVGAAYRHDLIRDYTTETSHTVALANGRQLQFSNSNRLVNNSGWDIGLS
                     KTGYISEAGRCLVLQAKIAARPVIIVLLDSWGRLTRIGDANRIKRWIESQSLRQPAG"
     gene            1616..2290
                     /locus_tag="AC158Q_05505"
     CDS             1616..2290
                     /locus_tag="AC158Q_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3339263.1"
                     /translation="MRILIVEDDPVLADGLTRSLRASDYAVDCVGDGGEADHVLAAQN
                     YDLVILDLGLPKLDGFEILRRLRRRSSRVPVLVLTARDALDDRVRGLDLGADDYLTKP
                     FDLPELEARLRALIRRGQSGGSSLITHGALTLDTGGRRATLGGEPLELSARELGVLEV
                     LMLRSGRVVNKEQLAEQLYGWDEEVGANAIEVYVHRLRKKLEPVGVTIRTIRGLGYLL
                     EKPPHG"
     gene            2283..>3239
                     /locus_tag="AC158Q_05510"
     CDS             2283..>3239
                     /locus_tag="AC158Q_05510"
                     /inference="COORDINATES: protein motif:HMM:NF020110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HIR3339264.1"
                     /translation="MAKVRTLRRQLIAWLSVPLAVLWLISGVVDYDIAKRFVNLAYDR
                     ALLEAALDIGRSLRQANNQIYIDLPEAALQMLETRESGRLHFLVTRTDGEYISGNPEL
                     PRAPEDDSVDRVRYYDDEFGGRAIRLVSLRHPIRPGFGQGAVLIHVGERLTVRSESAR
                     QLLLRIMVPQLLLILVAVFVVWYAVGRGLGPLTSLQRDVAARSDRDLSPLPEDKVPEE
                     VRPLVRAMNELLDRLNKALESRQRFVADAAHQLRTPIAGIKTQTELALRLTPAPDAQA
                     TLKQLHTATEQATRLVNQLLALARAEPGAGRVQRAEPLDLVEL"
BASE COUNT          563 a         1097 c         1061 g          518 t
ORIGIN      
        1 cgtagaaata cttgggcggc agcgccttgc gtgcgcgcga caggccggcg atgacgtcgc
       61 cgaggaagct gccggtatcg ggacgcaggt cgtgaaaggc aaagcggggg ccgtgctgat
      121 ccatgccctc ctcgcttctc gtatagttgt taaaaggcat ctcatgaacc gccacagcca
      181 cggaaacaca cggaaccaca cagaaattct tggagcacta cagggatgct aaccagaccg
      241 gggggccgtc cgtgtcactc cgtggcgaat cttgagacag gctctggtgg aacacggggt
      301 tgcgcgcgac tattcctgcg cctcgccgct cgccgcctcg ccgtcagccc gccggctggc
      361 gcaaggattg actctcgatc caccgcttga tgcgattggc gtcgccgatg cgagtcagcc
      421 tgccccacga atcgagcagc acgatgatca ccggccgcgc ggcgatcttc gcctgcagca
      481 cgaggcagcg gccggcctcg ctgatgtagc cggtcttcga caggccgata tcccagccgg
      541 agttgttgac cagccggttg gaattcgaga actgcagctg ccgcccgttg gcgagcgcga
      601 ccgtgtggct ggtttccgtg gtgtaatcgc ggatcaggtc atgccggtac gcggcgccca
      661 ccagggccac gaggtctcgc gcggtcgaga cgttcgcgct cgacaaaccg gtgccgtcca
      721 cgaagcgggt atgccacatg cccagccgga ccgctttctg gttcatcgcc gccacgaacg
      781 cgctcgtgcc gccgggatag gcccgggtca gcgccgcggc cgcgcgattc tcggatgcca
      841 tcagggcgag cttcagcagg tcggcgcgcg tcaactcggt gccgatgctc aaccgagagc
      901 gcgtgccctt gatcccgtcg cggtcctcgc tggtgatcgc gatgcgctcc tcgagcggca
      961 agccggactc caccaccacc agcgcggtca tcagcttggt gatggacgca atcgggacga
     1021 ccgcgtcgac attcttcgcg tacagcacct gcttcccccc ctgctcgacg accagggcag
     1081 tactggactt gagatcgagg accccggatt tggcgacccc ggccggctgc gcggcccggg
     1141 cctgcggcgg catggccggc gccagcgtca tgaggcatgc gatggcataa agcagcggct
     1201 tcagactccc ccctcccaca gcggccgtaa ggcgcccggc agcgcgaatt gaccaaaata
     1261 accgtcaaaa tacagggtta gcggccatat ttcaaccgca ttttaactgt ggccggcgcg
     1321 acgggtcaga gagacagcaa aaaaaatctc cgcgcgggac ggtgtgggag tcccggcgga
     1381 ggcaaagagg ccactgcaac catccgctca gcacagacaa ttgctactag caggcctccg
     1441 aggaggaaaa accgaagcga ctgccaaacc tgggcgggca cggcggccga gcatcccgcg
     1501 aaagtggaat gagcgtagtc ccgccgcctt acatgaaact tacacggaca tccgggttga
     1561 acaaatttaa ttctgtaccc gtatccttgt cgtctggttc acactccagg cggatatgcg
     1621 catcctcatc gtcgaagacg acccggtgct ggccgacggg ctgacccgtt cgttgcgggc
     1681 gagcgactac gcagtggatt gcgtcggcga cggcggcgag gccgatcacg tgctggccgc
     1741 ccagaactac gacctggtga tcctcgacct ggggctgccc aagctggacg gattcgaaat
     1801 cctgcgccga ttgcgccggc gcagttcgcg ggtgccggtg ctggtgctga ccgcccgcga
     1861 tgcgctggat gaccgtgtca ggggcctcga cctcggcgcc gacgattacc tcaccaagcc
     1921 gttcgacctg ccggagctcg aggcgcggtt gcgcgcgctc atccgccgcg ggcagtcggg
     1981 aggcagctcg ctgatcacgc acggggcgct cacgctcgac accggcggcc ggcgcgccac
     2041 gctgggcggc gagccgctcg agctctccgc gcgcgagctc ggggtgctgg aagtgctgat
     2101 gctgcgcagc ggccgggtgg tgaacaagga gcagctcgcc gagcagctct acggctggga
     2161 cgaggaagtc ggcgccaacg ccatcgaggt ctacgtgcat cgcctgcgca agaagctcga
     2221 gcccgtcggc gtcaccatcc gcaccatccg cggcctggga tacctgctcg agaagccgcc
     2281 acatggctaa ggtccggacc ctgcgccggc agctgatcgc ctggctatcg gtgccgctcg
     2341 cggtgctgtg gttgatcagc ggcgtggtgg attacgacat tgccaagcgc ttcgtgaacc
     2401 tcgcctacga ccgcgctctc ctcgaggccg cgctcgacat cgggcgcagc ctcaggcagg
     2461 ccaacaacca gatctacatc gacctgccgg aggcggcgct gcagatgctg gagacccgcg
     2521 aatccggccg cctgcatttt ctcgttaccc gcaccgatgg cgagtacatc agcggcaacc
     2581 ccgagctgcc gcgcgcgccg gaagatgaca gcgtggaccg cgtccgttac tacgacgatg
     2641 agttcggcgg ccgcgccatc cgcctggttt ccctgcgcca cccgatcagg ccgggattcg
     2701 gccagggcgc ggtgctgatc cacgtgggcg agcgcctcac cgtgcgctcc gagtccgcgc
     2761 gtcagctcct gttgcgcatc atggtgccgc agctgctgct gatcctcgtc gcggtgttcg
     2821 tggtctggta cgcggtcggc cgcggcctcg ggcccctcac cagcctgcaa cgcgacgtgg
     2881 ccgcccgctc ggaccgggat ctctcgccgc tgcccgagga caaggtgccg gaggaagtgc
     2941 ggccgctggt gcgcgccatg aacgagctgc tggaccgcct gaacaaggcg ctcgaatcca
     3001 ggcagcgttt cgtcgcggac gcggcacacc agctgcgcac gccgattgcc ggcatcaaga
     3061 cccagacgga gctcgcgctg cggttgacgc cggcgccgga tgcgcaggca acgctcaaac
     3121 aattgcacac cgcgaccgaa caggccaccc gtctcgtcaa tcagctgctc gccctggccc
     3181 gtgccgagcc gggtgccggg cgcgtccagc gcgcggaacc gctcgacctg gtcgaactc
//
LOCUS       DBNGSG010000206         4447 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3632120, whole genome shotgun sequence.
ACCESSION   DBNGSG010000206 DBNGSG010000000
VERSION     DBNGSG010000206.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4447)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3632120"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..1353)
                     /locus_tag="AC158Q_05515"
     CDS             complement(217..1353)
                     /locus_tag="AC158Q_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HIR3339265.1"
                     /translation="MERDQAVKFMHDLLRAMVAKKGSDLFITTGFPPAIKLDGKMTPV
                     ASQTLTQQHTLDLTRAIMNDKQAQEFEATKECNFAINPGGIGRFRVSAFMQQGRVGMV
                     LRTITTTIPKFEELNLPPVLKDIVMTKRGLIIMVGGTGSGKSTSLAAMIGYRNENSYG
                     HIITIEDPVEYVHEHKNCVITQREVGVDTDSWHAALKNTLRQAPDVILIGEIRDRDTM
                     DYAIAFAETGHLCMATLHANSANQALDRIINFFPEERKHQVLMDLSLNIRALVSQRLI
                     PKKESKGRVAAVEIMLNSPLISDLIFKGEVHEIKEIMKKSRELGMQTFDQHLFDLYEG
                     GHISYEDALRNADSVNELRLMIKLHGKEAKEKDLSTGIEHLNIV"
     gene            complement(1395..2435)
                     /locus_tag="AC158Q_05520"
     CDS             complement(1395..2435)
                     /locus_tag="AC158Q_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HIR3339266.1"
                     /translation="MDIAELLAFVVKNKASDLHLSSGLPPMIRVHGDVRRINLPAMEH
                     KDVHAMVYDIMNDGQRKFYEENLECDFSFAIPNLARFRVNAFVQQRGAAAVMRTIPSK
                     ILSLEDLKAPKIFADLADQPRGVVLVTGPTGSGKSTTLAAMINHKNENEHGHILTVED
                     PIEFVHESKKCLINQREVGPHTLSFNNALRSALREDPDVILVGEMRDLETIRLAMTAA
                     ETGHLVFGTLHTSSAAKTIDRIIDVFPAEEKEMMRAMLSESLRAVISQALLKTKDGAG
                     RVAAHEIMVGTPAIRNLIREAKVAQMYSAIQTGQNYGMQTLDQNLTDLVRRNVISVDE
                     ARTKAANKDNFK"
     gene            2526..3212
                     /locus_tag="AC158Q_05525"
     CDS             2526..3212
                     /locus_tag="AC158Q_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HIR3339267.1"
                     /translation="MTTIAANLQAVRARIARAAQGAGRNPEGITLVAVSKTFPAQHIA
                     QAQAAGQGAFGENYAREAVEKITKLGHLPLVWHFIGPIQSNKTGTIAAHFQWVHSVGR
                     EKIAQRLNAARADGLPPLNVLIQVNVSGEAGKSGISPGEEARLAEAIVRMPRLKLRGL
                     MAVPEPTPDTALQRRRFAQLRELKDRLVAHGYALDTLSMGMSDDLEAAIAEGATMVRI
                     GTAIFGSRKK"
     gene            3343..4155
                     /gene="proC"
                     /locus_tag="AC158Q_05530"
     CDS             3343..4155
                     /gene="proC"
                     /locus_tag="AC158Q_05530"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HIR3339268.1"
                     /translation="MKVTFIGGGNMAGAMIGGLIRKRWPRKAIRVVEVAQAARDRLER
                     ELRIKTQAALDARAAGSDCIVLAVKPQQLREVAVALKPRLASQLVITIAAGVRAADLS
                     RWLGGYRRIVRVMPNTPALVHAGISGLYAPAAVEAGARALAEKVLGAVGKTLWFEREE
                     DIDAVTAVSGSGPAYVFYFIEALERAARELGLAADTAHRLAVETFAGAVKLVAQSSEP
                     PETLRSRVTSKGGTTERALKSMEAERVKDAIVRAVRAAAERSRELGDELGKD"
     gene            complement(4261..>4447)
                     /locus_tag="AC158Q_05535"
     CDS             complement(4261..>4447)
                     /locus_tag="AC158Q_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339269.1"
                     /translation="PNIMKRALRCHDFGSGREASRSQYVDIGKASDKAMGQNSCQPLD
                     YRWIFHCLRRGCGQNRR"
BASE COUNT          763 a         1380 c         1463 g          841 t
ORIGIN      
        1 ggtgaacagc gccgccccga acgccgagat gccgaagacg acgtaggacc cgaataccac
       61 cgccgcggcg atggcgaaca tcggaaggtc gaacggaccg gtcatgtgca ataaaaaagc
      121 gccggaatgt catccgacgc ttttgttacg ccggttgagc gggtggtatc ggtccgctcg
      181 aaccggcgta tctgatccgg cgcttcgcgt ccaggatcag acgatgttca ggtgctcgat
      241 ccccgtcgac agatccttct ccttggcttc cttgccgtgg agcttgatca tcaggcgcag
      301 ctcgttgacc gaatcggcgt tcctcagcgc atcctcgtag ctgatgtgcc cgccctcgta
      361 gaggtcgaac aggtgctggt cgaaggtctg catgccgagc tcgcgcgact tcttcatgat
      421 ctccttgatc tcgtgcacct cgcccttgaa gatgaggtcg gagatcagcg gcgagttcag
      481 catgatctcg accgccgcca cccgcccctt gctctctttc ttcgggatga ggcgctgcga
      541 gaccagcgcg cggatattga gcgagaggtc catcagcacc tggtgcttcc gttcctcggg
      601 gaagaagttg atgatgcggt cgagcgcctg gttggcgctg ttggcgtgca gcgtcgccat
      661 gcacaggtgg ccggtttccg cgaaggcgat cgcgtaatcc atggtgtcgc ggtcgcggat
      721 ttccccgatc aggatgacat ccggcgcctg gcgcagcgtg ttcttcagcg ccgcgtgcca
      781 tgaatccgtg tccacgccca cctcgcgctg ggtgatgacg cagttcttgt gctcgtgcac
      841 gtactcgacc gggtcttcga tagtgatgat gtggccgtag ctgttctcgt tgcgatagcc
      901 gatcatcgcc gcgagcgagg tggatttccc ggaaccggtg ccgccgacca tgatgatcag
      961 gccgcgcttg gtcatcacga tgtccttcag caccggcggc aggttgagct cctcgaactt
     1021 cgggatcgtg gtggtaatgg tgcgcagcac catgccgacg cgcccctgct gcatgaaggc
     1081 gctcacgcgg aagcggccga taccgcccgg gttgatcgcg aagttgcact ccttggtagc
     1141 ctcgaattcc tgcgcctgct tgtcgttcat gatggcgcgg gtcagatcca gggtgtgctg
     1201 ctgcgtgagt gtctggctcg cgaccggcgt catcttgccg tcgagcttga tcgcgggcgg
     1261 aaagccggtg gtgatgaaaa ggtccgatcc cttcttcgcc accatcgccc tcaacaggtc
     1321 gtgcatgaac ttgactgcct ggtctctttc catatgggtg ctcccgcttt gtctgcgacc
     1381 gcgcggcggc cgccttattt gaagttgtcc ttgttcgccg ccttggtgcg cgcctcgtcc
     1441 accgagatga cgttgcgcct gaccaggtcc gtcaggttct gatcgagcgt ctgcatcccg
     1501 taattctgcc cggtctggat cgcggaatac atctgcgcga ccttggcctc gcggatcagg
     1561 ttgcggatcg ccggcgtgcc gaccatgatc tcgtgcgccg ccacccggcc ggcgccgtcc
     1621 ttggtcttca acagcgcctg cgagatcacc gcgcgcagcg attccgacag catcgcgcgc
     1681 atcatttcct tttcctcggc cgggaacacg tcgatgatgc ggtcgatggt cttcgccgcg
     1741 gaactcgtgt gcagcgtgcc gaacaccagg tggccggtct ccgccgccgt catggcaagc
     1801 cggatcgttt ccaggtcgcg catctcaccc accaggatca cgtccggatc ctcccgcagg
     1861 gcgctcctca gcgcgttgtt gaacgacagg gtgtgcgggc cgacctcgcg ctggttgatc
     1921 aggcacttct tcgactcgtg cacgaactcg atcgggtcct ccaccgtgag aatgtggccg
     1981 tgctcgtttt cgttcttgtg gttgatcatg gcggcgagcg tggtcgactt gccggagccg
     2041 gtcgggccgg tcaccagcac cacgccgcgc ggctggtcgg caaggtcggc gaaaatcttc
     2101 ggggccttca aatcctccag cgacagaatc ttggagggaa tggtgcgcat caccgccgcc
     2161 gcgccgcgct gctgcacgaa ggcgttcacc cggaagcgcg caaggttggg aatcgcgaac
     2221 gagaagtcgc actcgaggtt ttcctcgtag aacttgcgct gcccgtcgtt catgatgtcg
     2281 tacaccatgg cgtgcacgtc cttgtgctcc atggccggca ggttgatgcg ccgcacgtcg
     2341 ccgtgcacgc ggatcatcgg cggcagcccc gaggaaaggt gcaggtcgga ggccttgttc
     2401 ttgactacga aagccagcag ttccgcgatg tccatttata atgctccgtg atcgggtttg
     2461 gcggcggaag gtgagttaag tttttgattt tacgccggtc gccatctcgg cgcgatgctg
     2521 aaattatgac cacaatcgcc gccaacttgc aagccgtgcg cgcccgcatt gcccgggcgg
     2581 cgcagggcgc cggacggaat cccgaaggga tcacgctggt cgcggtgagt aaaaccttcc
     2641 ctgcccagca catagcgcag gcgcaggccg ccggccaggg ggcgttcggg gagaactacg
     2701 cccgggaggc cgtggaaaaa atcacgaaac tcggccatct gcccctggta tggcatttca
     2761 tcggcccgat acagagcaac aagaccggga cgatcgcggc ccatttccag tgggtacaca
     2821 gcgtggggcg cgagaaaata gcgcagcgcc tgaatgccgc gcgggcggat ggactgcctc
     2881 ccctcaacgt tctcatccag gtgaacgtca gcggcgaggc cggcaagagc ggcatctccc
     2941 cgggagagga agcaaggctc gcggaggcca tcgtgcggat gccgcggctg aagctgcgcg
     3001 gactgatggc ggtgccggaa cccacgccgg acacggcgct gcagcgccgg cgcttcgcgc
     3061 agctgcgcga gctgaaggac aggctggtcg cccacggcta cgcgctcgac acgctgtcga
     3121 tgggcatgtc ggacgatctc gaggcggcca tcgccgaagg cgcgacgatg gtgcgcattg
     3181 gaaccgcgat cttcggatcg cggaagaagt gactcgttgc tcgttcctcg ttgctcgttt
     3241 ttcccttgat gcttgacccg gagccatccg ccggtcggcg gaggctgtta acataagcct
     3301 gatgcaagac gagaaacgag aaacgaataa cgagtcactc ttatgaaagt gaccttcatc
     3361 ggcggcggca acatggcggg cgccatgatc ggcgggctga tccgcaagcg ctggccgcgc
     3421 aaggcgatcc gggtggtgga agtcgcgcag gcggcgcgcg acaggctgga gcgcgagctg
     3481 aggatcaaga cccaggcggc acttgacgcg cgggcagccg ggagcgactg catcgtgctg
     3541 gcggtcaagc cgcagcagct gcgcgaggtc gccgtcgcgc tgaagcctcg cctcgcctcg
     3601 cagcttgtca tcaccatcgc ggccggcgtc cgggcggcgg atctctcgcg ctggctggga
     3661 ggataccggc gtatcgtgcg cgtcatgccc aatacgccgg cgctcgtgca cgccggcatc
     3721 tccggcctct acgcgcccgc ggctgttgaa gccggcgccc gcgccttggc agagaaggtc
     3781 ctgggggccg tgggcaaaac cctgtggttc gagcgcgagg aggacatcga cgccgtgacc
     3841 gccgtctccg gcagcggtcc cgcctacgtg ttctacttca tcgaggcgct ggagcgcgcc
     3901 gcccgggaac tggggcttgc ggcggatacc gcgcaccggc tcgcggtcga gaccttcgcc
     3961 ggcgcggtca agctcgtggc gcagtcgagc gagccgccgg agacgctgag gagccgcgtt
     4021 acctcgaagg gcgggaccac ggagcgcgcg ctgaaatcca tggaagccga gcgcgtaaag
     4081 gacgcgatcg tgcgcgcggt gcgcgccgcg gccgaacgct cgcgcgagct gggcgacgag
     4141 ctggggaaag actaagagtc cctaacataa tgaaacacgc gtgcgttgcg agccaaaatg
     4201 ggtgatgacg cgaaggacgg cgcaggtcct atgtcgatag cgccaagccg gccgacaaag
     4261 tcagcgacga ttttggccgc aaccccggcg caagcaatgg aatatccaac ggtaatccaa
     4321 gggctggcac gagttttggc ccatggcttt gtcgctcgcc ttgccaatat cgacatattg
     4381 gctgcggctc gcttcgcgcc ctgagccaaa atcgtgacag cgcagcgcgc gtttcattat
     4441 gttaggg
//
LOCUS       DBNGSG010000207         5278 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4142372, whole genome shotgun sequence.
ACCESSION   DBNGSG010000207 DBNGSG010000000
VERSION     DBNGSG010000207.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5278)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5278)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5278
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4142372"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="AC158Q_05540"
     CDS             complement(<1..296)
                     /locus_tag="AC158Q_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase H family protein"
                     /protein_id="HIR3339270.1"
                     /translation="MSEVIEIYTDGACKGNPGVGGWGALIRRNGRPPQELHGGERLTT
                     NNRMELLAVIRALETLEPGTRARVHTDSQYVQLGIKEWIHSWKRRGWRTADRKPV"
     gene            complement(401..1189)
                     /locus_tag="AC158Q_05545"
     CDS             complement(401..1189)
                     /locus_tag="AC158Q_05545"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3339271.1"
                     /translation="MSNHDTARAAAEWLTTPLGQYLLAREQAYFDRTAADIFGYIALQ
                     LGLPQIDLLRANRIACRLHADPSGPAELRADFRDLPVASNSIDLVVLAHVLEFSENPH
                     QVLREVARVLLPEGQMVIACFNPWSLWGFRRVFGKKVDYPWNGRFINLPRLKDWLTLL
                     ELEITGGQMCCYSPPCATEKWLQRFAFMEAAGDRWWPITGGIYFLQAVKRVRGMRLIM
                     PRWSDRLAPKKDLAPVPKKVARPDEAVVARNGVSDKTSQMVRPR"
     gene            1250..2020
                     /gene="gloB"
                     /locus_tag="AC158Q_05550"
     CDS             1250..2020
                     /gene="gloB"
                     /locus_tag="AC158Q_05550"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HIR3339272.1"
                     /translation="MNRYEIIPLRAFSDNYIWTLRDENCAAVVDPGDARPVLEYLARE
                     KLELVAILNTHHHADHVGGNAGLLGRWKVPVFGPRDPRIGEVTHGLGNGKRCTLPHFG
                     IELEVSEIPGHTRSHISFHGDGMLFCGDTLFAAGCGRLFEGTPLQMHDSLSRLMRLPD
                     DTRVFCGHEYTVSNVRFARAVEPGNAALRELEARAKQQRDRDLPTLPSTIAQEKATNP
                     FVRVREPEVIASANRYAGKTLNDPVSVLAAIREWKNSF"
     gene            2332..3606
                     /locus_tag="AC158Q_05555"
     CDS             2332..3606
                     /locus_tag="AC158Q_05555"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HIR3339273.1"
                     /translation="MQTAEPDALARTETAAAPAAPARSVEPPAPAPEPARAPEPPPPP
                     TDVWDRIRAGLRLEPISSPLVQDWERYYSQRPEYFARMVERSSRFLYHVIEEVERRKM
                     PAEIALLPMIESAYNPQAYSRAHASGMWQFIPSTGKLYGLNQDFWYDGRRDVLAATSA
                     ALDYLEKLHGMFNDWNLALASYNWGEGAVARAISRNQARGLPADYESLTMPAETRNYI
                     PKLQAVKNIIANPEQYGITLASVPNRPYFATVTTSRHIDVRLAASLAEISLEEFRSLN
                     PAHNKPVIKSNGQETIVLPIEKVAVFQSNLEAHDQPLATWQIYTVKRSDKPEQIAAKH
                     GMTLAQLKEINGITGGKNIVAGQTLLVPIKGTAEPNLPDLPAPKITRTTYRGKQYRCT
                     MKTSNGQRRVVPCAAPKKPAAAKTAKKPSIKM"
     gene            complement(3704..4075)
                     /locus_tag="AC158Q_05560"
     CDS             complement(3704..4075)
                     /locus_tag="AC158Q_05560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR3339274.1"
                     /translation="MGDLSNHRELVVWQEAIKLVVLVYRETAGFPREELFGLMAQLNK
                     SAGSIPSNIAEGAGRNSSKELIQYLGIASGSRAEVDTHLEVALQLGFIRPNSAVFGQS
                     ERVGKLLVGLRKSLRRRYTAT"
     gene            complement(4211..5269)
                     /locus_tag="AC158Q_05565"
     CDS             complement(4211..5269)
                     /locus_tag="AC158Q_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3339275.1"
                     /translation="MHRVGRSPARTHAGASIETPDSLPHPSDARPLLEVRDLKVHFPV
                     HKGLFKRVVAHVRAVDGVSLAIHAGRTLGLVGESGCGKTTVGKGILRLTEPTGGAVLF
                     DGTDLARLRRGELRARRKDLQIIFQDPYSSLNPRMRVLETLEEGMAALGVGAGPGERQ
                     ARVDALLAEVGLAPDVKYRYPHEFSGGQRQRIAIARALSVEPRLIVCDEPTSALDVSV
                     QAQILNLLKDLQRKKGLAYLFITHNIAVVEFLAHEVAVMYLGRIVEHGSVNEVLSGAK
                     HPYTQALLSAVPVVDPGTRREVIRLQGDLPSPVEPPAGCHFHPRCPAAFARCRDAYPG
                     ISRFTDSHTAACHLYAVK"
BASE COUNT         1037 a         1748 c         1606 g          887 t
ORIGIN      
        1 accggtttcc tgtcggcggt gcgccagccg cggcgcttcc acgaatggat ccattccttg
       61 atccccagct gaacgtactg ggaatcggta tgcacgcggg cgcgggtgcc gggctcgagc
      121 gtctcgagcg cgcgtatcac cgcgaggagc tccatgcggt tgttggtcgt caatcgctcc
      181 ccgccgtgca gttcctgcgg tggcctccca ttgcgcctga tcagcgcgcc ccacccgccc
      241 acgccgggat tgcccttgca ggcgccgtcg gtgtagatct cgatgacttc gctcatgaca
      301 gcgtctcagg taaagcatct caggtaaagc gtctcaggtg tggcgtcccg gacagaacgc
      361 accggaaagg tgggagctgg accgcagtct ctgagacgca ttatctggga cgcaccatct
      421 gcgacgtttt atctgagaca ccgtttcggg caacgacggc ctcatccggc cgcgcgacct
      481 tcttgggaac gggggcaaga tccttcttgg gagccagccg gtcgctccat ctcggcatga
      541 tgagccgcat gccgcgcacg cgcttcaccg cctgcaggaa ataaataccg cccgtgatcg
      601 gccaccagcg gtcgcccgcc gcttccatga aagcgaagcg ctgcagccat ttttcagtgg
      661 cgcagggcgg cgaatagcag cacatctgcc cgcccgttat ttccagctcc agcagcgtga
      721 gccagtcctt cagccgcggg aggttgatga aacggccgtt ccacgggtag tccactttct
      781 tgccgaacac gcgccggaag ccccacaggc tccaggggtt gaagcaggcg atcaccatct
      841 ggccttccgg cagcagcacc cgcgccacct cgcgcaggac ctgatgcggg ttctcgctga
      901 actcgaggac gtgcgccagc acgacgagat cgatgctgtt gctggcgacc ggcaggtcgc
      961 ggaaatcagc ccgcagttcc gcgggacccg aagggtcggc atgcagccgg catgcgatgc
     1021 ggttcgcgcg cagcaggtcg atctgcggca gcccgagctg cagcgcgatg tagccgaaga
     1081 tgtcggctgc cgtcctgtcg aagtacgcct gctcccgcgc gagcaggtac tgtccgagcg
     1141 gcgtggttaa ccactccgcg gcggcacggg ccgtgtcatg attggacatg acgtcagttt
     1201 ataatcgcca cacggtattg gaaagccgct tcgcgagtca catttctcca tgaatcgata
     1261 cgaaatcatt ccgcttcgcg ccttttcgga caattacatc tggaccctcc gtgacgaaaa
     1321 ttgcgccgcg gtcgtggacc cgggcgatgc gcggcccgtg ctcgagtacc tggctcggga
     1381 aaaactcgaa ctggtcgcga ttctcaacac ccaccaccac gccgatcacg tcggtggaaa
     1441 cgccgggctg cttgggcgct ggaaggtgcc ggtgttcgga ccgcgcgatc cgcgtatcgg
     1501 cgaggtcacg cacggcctgg gcaacgggaa gcgctgcacg ctaccgcact tcggcatcga
     1561 gctcgaggtg tcggaaatcc ccgggcacac gcgcagccac atctcgtttc acggcgacgg
     1621 catgctgttc tgcggcgaca ccctgttcgc cgcgggctgc gggcggctgt tcgagggcac
     1681 accgctgcag atgcacgatt ccctctcccg cctgatgcgg ctgccggacg acacccgggt
     1741 cttttgcggc cacgagtaca cggtgtcgaa tgtccgcttc gcccgggcgg tcgagcccgg
     1801 aaacgcggcg ctgcgcgaac tggaagccag ggcgaaacag cagcgcgacc gggatctccc
     1861 cacgctgcct tccaccatcg cgcaggagaa ggcgaccaac cccttcgtcc gggtgcgcga
     1921 gcccgaggtc atcgcctctg ccaaccgata tgccggcaag acgttaaatg atccggtgag
     1981 cgttctggcg gcgatccggg aatggaaaaa ctcgttctga acggcgtgat tggtgaatgg
     2041 tgagcggtga gtggtgcata aaccctcccg caacccgcaa acgtacggcg gaagcgggct
     2101 ccaatcgcca ttcactactc accctgcacg cacccgaagt taggaaactg cttgcagttt
     2161 cgatcagatt atcatgattt ttaagcatat ttatcttgac ggaactagtg gtcttggcta
     2221 ctatcccctc gtcctgtaaa aggcagggtt ggtaacgagc gtgcccctat gctcataaga
     2281 aacctctcta tcgctcttgc ggccgtcctg gtgacggccg gatgcgccca gatgcaaacg
     2341 gctgagccgg atgcgctggc acgaaccgag acggcggccg ctcctgccgc gccggcgcgg
     2401 agcgtggagc cgccggcccc cgcaccggaa ccggcacgcg cgccggagcc acctcccccg
     2461 cccaccgacg tctgggatcg cattcgcgcc ggcctgcgtc tcgagccgat ttcgagcccg
     2521 ctggtgcagg actgggagcg gtattactcg cagcgcccgg agtactttgc gcgcatggtg
     2581 gagcgcagca gccgcttcct ctatcacgtg atcgaggaag tcgagaggcg caagatgccg
     2641 gccgagatcg cgctgctgcc gatgatcgag agcgcctaca acccgcaggc ttattcgcgc
     2701 gcgcacgcct ccggcatgtg gcagttcatt ccctccaccg gcaagctcta cggattgaac
     2761 caggacttct ggtacgacgg gcggcgcgac gtgctggcgg cgaccagcgc cgcgctcgat
     2821 tacctggaga agctccacgg catgttcaac gactggaacc tcgctctcgc ctcctacaac
     2881 tggggcgagg gcgcggtcgc gcgcgccatc tcgaggaacc aggcaagggg gctgcccgcc
     2941 gactacgaga gcctcaccat gccggcggaa acgcgcaact acatccccaa gctccaggcc
     3001 gtcaagaaca tcatcgcaaa ccccgagcag tacggcatca cgctcgccag cgtcccgaac
     3061 cgcccgtatt tcgccacggt cactaccagc cgccacatcg acgtgagact cgccgcgagc
     3121 ctcgccgaga tatcgctgga ggaattccgc tccctcaatc ctgcccacaa caagccggtg
     3181 atcaagtcca acggccagga gacgatcgtg ctgccgatcg agaaggtcgc cgtgttccag
     3241 agcaacctgg aggcccacga ccagccgctg gcgacatggc agatctacac cgtcaagcgc
     3301 agcgacaagc cggaacagat cgcggcaaag cacgggatga cactggccca gctgaaggaa
     3361 ataaacggta tcaccggcgg gaaaaatatc gtcgccggcc agaccctgct cgtgccgatc
     3421 aagggcaccg ccgaacccaa cctgcccgac ctgcccgcgc ccaagatcac gcggacgacc
     3481 taccggggca agcagtaccg gtgcaccatg aagacgtcga atggacagcg ccgggtcgta
     3541 ccctgcgctg cgccgaagaa gcccgcggcc gccaagacgg caaaaaaacc cagcatcaag
     3601 atgtagctga agtcgcccgc gaggagcgcg gcgccggatg agctagccgc gcgcccagaa
     3661 aggcgtgatt ggtgattggt gaatagtgat tggacgctgt gaatcaggta gccgtgtagc
     3721 gccgtcttag cgacttgcgc aatccaacaa gcagctttcc cacgcgttcc gactgaccaa
     3781 ataccgccga attcggccgg ataaagccaa gctgaagcgc gacttcgaga tgagtatcca
     3841 cctcggctct ggatccgctt gcaatcccca agtactggat caactctttc gatgagtttc
     3901 tgccagcgcc ctccgcaata ttggatggga tagaacccgc gctcttattc agctgggcca
     3961 ttaggccgaa caattcttcc cggggaaaac ccgctgtttc cctgtagaca agcacaacga
     4021 gcttgatagc ttcctgccac acgacaagct cacgatgatt ggaaagatca cccatattcc
     4081 gcccccccat gatgcatgga tatagaggct ataacgagcc aacagagtcc gggtttacag
     4141 gtttttcaat tggctccgaa aggggtgaaa aaaagtccat tcaccactca ccaatcacca
     4201 ttcacgcctc ctacttcacc gcgtaaaggt gacaagcagc agtatgtgaa tccgtaaaac
     4261 gcgaaatgcc cggataagcg tcgcggcagc gcgcgaacgc cgccggacac cgcggatgaa
     4321 aatgacaacc cgccggcggc tcgaccggcg acggcaggtc gccctgcaga cggatcacct
     4381 cgcgccgggt tcccggatcc accaccggca ccgccgacag cagcgcctgc gtgtagggat
     4441 gcttcgcgcc ggacagcacc tcgttgacgc tcccgtgctc gacgatgcgc ccgagataca
     4501 tcaccgcaac ctcgtgcgcg aggaattcga ccacagcgat attgtgggtg atgaagagat
     4561 aagccagtcc tttctttctc tgcaggtcct tcagcaggtt caggatctgg gcctgcaccg
     4621 agacgtcgag cgcgctggtc ggctcgtcac acacgatgag ccggggctcg accgacagcg
     4681 cgcgcgcgat cgcgatgcgc tggcgttgcc cgccggagaa ctcgtgcggg tagcggtatt
     4741 tcacgtcagg ggcgagcccc acctcggcga gcagcgcatc cactcgcgcc tgccgctccc
     4801 cgggcccggc gcccacgccc agcgccgcca tgccctcctc cagcgtttcc agcacccgca
     4861 tgcgcgggtt gagcgaggag tacggatcct ggaagatgat ctgcaggtcc ttgcggcggg
     4921 cgcgcaattc gccacgcctc aatcgcgcaa ggtccgtccc gtcgaacagc accgcgccgc
     4981 cggtcggctc ggtaagccgc aggatgccct tgcccaccgt ggtcttgccg caacccgact
     5041 cgcccaccag gcccagtgtc cggccggcgt ggatggcgag ggacacgcca tccacggcgc
     5101 gtacgtgcgc caccacgcgc ttgaacaggc ccttgtggac gggaaagtgc accttgaggt
     5161 cgcggacctc gaggagcggg cgcgcgtccg agggatgagg gagtgagtcc ggggtttcga
     5221 tggacgcacc tgcgtgcgtc cgagccggcg aacgcccgac gcgatgcagc gcgtcggg
//
LOCUS       DBNGSG010000208         2301 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3293049, whole genome shotgun sequence.
ACCESSION   DBNGSG010000208 DBNGSG010000000
VERSION     DBNGSG010000208.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3293049"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="AC158Q_05570"
     CDS             <1..1084
                     /locus_tag="AC158Q_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR3339276.1"
                     /translation="MEFWQFHPTGVAGAGVLITEGVRGEGGYLLNKNGERFMERYAPN
                     AKDLASRDVVSRAMATEIKEGRGAGKDGEFILLKLDHLGEETINKRLPGIREIAKKFA
                     NVDPVKEPIPVVPTCHYQMGGIPTNYHGQVVAPAGDNPEAVIRGFYAAGECSCVSVHG
                     ANRLGCNSLLDLLVFGKSAAEQVIRDVKGTGAAHGALPADAGELTRARLARLEGQKSG
                     ERVSDVGFEMRRVMQLHCGVFRFPDLLVEGVTKIKDVAARGARTEIQDKSKTFNTARV
                     EALELDNLLDTAVASLVSAEARKESRGSHDRADFHDRPGYPNGRDDKVWMKHTLWYKE
                     GSRLAYKPVHLKPLSVEPFEPKARVY"
     gene            1181..1876
                     /locus_tag="AC158Q_05575"
     CDS             1181..1876
                     /locus_tag="AC158Q_05575"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (quinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HIR3339277.1"
                     /translation="MRFSIYSYNPEADEKPRMQDYDVALEPTDRMLLDALVRIKTHDD
                     SLAFRRSCREGVCGSDAMNINGKNRLACVTKITELREPVVIRPLPGQPVVRDLIVDMS
                     QFFKQYHSIKPYLINNDPPPEKERLQSPEDREELNGLYECILCACCTTACPSFWWNPD
                     KYVGPAGLLQAYRFLADTRDQATGERLDDLEDPYRLFRCHSIMNCVDVCPKGLNPMLA
                     IGKIKDLLVKRTV"
     gene            1892..2122
                     /locus_tag="AC158Q_05580"
     CDS             1892..2122
                     /locus_tag="AC158Q_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006105 - succinate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0034552 - respiratory chain complex II
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HIR3339278.1"
                     /translation="MAEFDRIRWRCRRGLLELDLVLNRFLEHRFERLDAEERRLFNEL
                     LDEPDNDLLDLALGRREPTPRYRAVVEMLRAG"
BASE COUNT          462 a          693 c          768 g          378 t
ORIGIN      
        1 catggagttc tggcagtttc acccgacggg cgtggccggg gcgggggtgc ttatcacgga
       61 aggcgtgcgc ggggagggcg gctatctcct caacaagaac ggcgagcgtt tcatggagcg
      121 ttacgcgccc aacgccaagg acctggcgag ccgcgacgtg gtctcgcgcg cgatggcgac
      181 cgagatcaag gaaggccgcg gcgcgggcaa ggacggtgaa ttcatcctgt tgaagctcga
      241 ccacctcggc gaggaaacca tcaacaagcg cctgccgggc atacgcgaaa tcgccaagaa
      301 atttgccaac gtggacccgg tgaaggaacc gatcccggtg gttccaacct gccattacca
      361 gatgggcggc atcccgacca actatcacgg ccaggtggtg gcacccgcgg gtgataatcc
      421 ggaggcggta atccggggtt tctatgcagc aggggaatgc tcctgtgtgt cggtgcatgg
      481 cgcaaaccgc ctgggctgca attcgttgct ggacttgctg gtgttcggca agtccgccgc
      541 cgagcaggtg attcgcgatg tcaagggaac cggcgcggcc catggcgccc ttcccgcgga
      601 cgccggtgag ctcacgcgtg cccggctcgc ccggctcgaa ggacagaaga gcggcgagcg
      661 cgtgtcggac gtcggcttcg agatgcgtcg cgtcatgcag cttcattgcg gcgtgttccg
      721 ctttcccgat ctgctggtcg aaggcgtgac gaaaatcaag gacgtggcgg cacggggcgc
      781 cagaacggaa atccaggaca agagcaagac gttcaacacc gcccgggtgg aggcgctcga
      841 gctcgacaac ctgctggata ccgccgtcgc ctcgctggtc tcggcggagg cccgcaagga
      901 gagccgcggc tcacacgacc gcgcggattt tcacgaccgg ccgggatacc cgaacggccg
      961 cgatgacaag gtctggatga agcacacgct gtggtacaag gaaggcagcc ggctggcgta
     1021 caagccggtg cacctcaagc ctctcagcgt cgagccgttc gagcctaaag cgcgtgttta
     1081 ctgaaaaagt gattcgttac tcgttactgg agccctactg gaacagcaca agacacgaca
     1141 cgagaaacga gaaacgagcc acccgttcac ggagcattta atgcgatttt ccatctacag
     1201 ctataacccc gaggccgacg aaaagccgcg gatgcaggac tacgacgttg cgctggagcc
     1261 gaccgaccgg atgctgctcg atgcgctggt gcgcatcaag acgcatgatg actcgcttgc
     1321 gtttcgccgt tcctgccgcg aaggggtatg cggctccgat gcgatgaaca tcaacggcaa
     1381 gaaccggctc gcctgcgtca ccaagatcac cgaactgcgg gagccggtgg tgatacggcc
     1441 gttgccgggc cagccggtgg tgcgcgacct gatcgtggat atgtcgcagt tcttcaagca
     1501 ataccactcg atcaagccgt acctgatcaa caacgacccg ccgccggaga aggagcggct
     1561 gcagtcgccg gaagaccggg aagaactgaa cggcctctac gagtgcatcc tgtgcgcctg
     1621 ctgcacgacc gcctgcccct ccttctggtg gaacccggac aagtacgtcg gcccggccgg
     1681 cttgctgcag gcctaccgct tcctggccga cacgcgcgac caggcgaccg gcgagcggct
     1741 ggacgatctc gaggatccgt accgcctgtt tcgctgccat tccatcatga attgcgtgga
     1801 cgtgtgccca aaggggttaa atcccatgct ggcgatcggc aagatcaagg acctgcttgt
     1861 gaaacgaacg gtgtaggggc ggcaaccgac catggccgag ttcgaccgca tacgctggcg
     1921 ctgccgcaga ggcctgctgg aactcgacct cgtcctgaat cggttcctgg agcaccgctt
     1981 cgagcgcctc gatgccgaag aacggcggct gttcaatgaa ctgctggacg agccggataa
     2041 cgacctgctc gacctggcgc tggggcgccg ggagccgacg ccgcgctacc gggcggtggt
     2101 ggagatgctg cgcgcgggct gatccggctt cgcgcgcgga gggtgtgggg aacggcacga
     2161 gccgtttgag agtaagagcc tatctcaata ataggcgcgt gatgcgttgt ggccaggacg
     2221 ggatggatgc gaggcgcacg gcgcaggccc tatgtcgata gggtcaagcc gggcaacgaa
     2281 gcagacgccc gtcctggccg c
//
LOCUS       DBNGSG010000209         2600 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2274389, whole genome shotgun sequence.
ACCESSION   DBNGSG010000209 DBNGSG010000000
VERSION     DBNGSG010000209.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2274389"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            371..946
                     /locus_tag="AC158Q_05585"
     CDS             371..946
                     /locus_tag="AC158Q_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3339279.1"
                     /translation="MTHPVPLERLPRIPPEKMTGAQKKAAAEIAAGPRGELRGPFIAL
                     LRSPDLMARVQHLGEYLRFQCPLDRRVMEFATLIAARFWSQSYEWQSHYQHAMKAGLR
                     PAIADALAEGRRPEGMMEDEEIAYELLTESLHNKCVSDATYARAVARFGEAGVVDLVA
                     LAGYYLMLAMVLNMARIALPAGRQPLKPFPC"
     gene            1186..1650
                     /locus_tag="AC158Q_05590"
     CDS             1186..1650
                     /locus_tag="AC158Q_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3339280.1"
                     /translation="MSTQSNTPAAGAKEALTQEFNGKPVTASVFGYQRPEKLEKGKGI
                     VRLCRSDIVYSTVQVLQDGGENNLHAHPAQDGIWIVLKGRVKFYGKDDAVLAELGPMQ
                     GIHIPRGFFYWFESASPEMLEILQVEAIDKSVKNERLNATPQKDFATEIQRF"
     gene            1778..>2600
                     /locus_tag="AC158Q_05595"
     CDS             1778..>2600
                     /locus_tag="AC158Q_05595"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3339281.1"
                     /translation="MRSIQIAAAFVACLSGLAAHAAESWPVRPVRLIVTSPPGGANDT
                     QARVLGAKLAEQLGQAVVIDNRGGGSGMIAAAIVAKSAPDGYTLLFGTNSTYSVNPSL
                     FPQAPYDTLRDFAPVAVTVMTPHVLLVHPSLPAATVKELIALARAQPGRLNYASSGTG
                     SAFHLGMELLKTAAGVAIVHVPFKGSALSANAMIAGEVQMMLVGMPTGMPLAKSGKAR
                     VLGVATPARSVLAPELPTIAEAGVPGYGYQSWFGVVAPAGTPRPVITRLHEGFDRP"
BASE COUNT          449 a          862 c          852 g          437 t
ORIGIN      
        1 tggtcggcat cacgatgggc gagatcttct atctcaagga cctcgccgac gactgcgcgg
       61 ccgaccgggt ctacgaattc ctgttctgcg gcccgccgct tcccatcacc ggcgcggtcg
      121 ggtcgccgat caatcccatg gccgtcaagt aacgagtgtc gagtgtcgag tgtcgggtgt
      181 cgggtgtcga atgtcgaggg aagaaagcca ccattacgtt ttttcacccg acactcattc
      241 ctcgacactc aatccccgac actccgtccc ttctaactcc ctcgacactc aatcctcgac
      301 actttttccc tcatccctga ttgccgcatc accgatcgca acgaccgtta aattacagaa
      361 gggagtattg atgacccacc ccgttcccct tgagcgcctg ccgcggattc cgccggaaaa
      421 gatgaccggg gcgcagaaga aggccgcggc cgaaatcgcg gcgggcccgc gcggcgagtt
      481 gcgcgggccg ttcatcgcgc tgctgagaag ccccgacctg atggcgcggg tgcagcacct
      541 cggcgaatac ctgcgcttcc agtgccccct cgatcgccgc gtcatggagt tcgcgacgct
      601 gatcgccgcg cgcttctggt cgcagtccta cgagtggcag tcgcactacc agcacgcgat
      661 gaaggcgggc ttgaggcccg cgatcgcgga cgccctcgcc gagggacggc gcccggaggg
      721 catgatggag gacgaggaga tcgcctacga gctgctgacc gagtcgctcc acaacaagtg
      781 cgtgagcgac gcgacctacg cgcgcgcggt cgccaggttc ggcgaggcgg gcgtggtgga
      841 tctcgtcgcg ctcgcgggct attacctgat gctggcgatg gtgctcaaca tggcgcgcat
      901 cgcgctgccg gcgggcaggc agccgctcaa gccgtttccc tgttagcggg aagagcgtga
      961 ttcgtctccc accacgccgg cgcgacgcgc caccccgccc gtcccgccgc gctgcatcgc
     1021 gtcggtcgtt cgccggctca aatgtgcgca ggcacatttt tcgaaacccc ggctcaccct
     1081 ccggcagaag aggatgccgt gagtcttgaa tagaccggtt tcgttgcccg ccctgcgcgg
     1141 cgggcgtatt atttgccggc ccaattctga aaagaggatg cccgcatgag cacacagagc
     1201 aacacgcccg ccgccggcgc gaaggaagca ttgacgcagg agttcaacgg caagccggtc
     1261 acggcttccg tgttcggcta ccagaggccg gagaagctgg agaagggcaa gggcatcgtg
     1321 aggctgtgcc gcagcgacat cgtgtattcg acggtgcagg tgctgcagga cggcggcgaa
     1381 aacaacctgc acgcgcatcc cgcgcaggac ggcatctgga tcgtgctgaa ggggcgcgtg
     1441 aaattctacg gcaaggacga tgcggtgctg gccgagttgg ggccgatgca gggcatccac
     1501 atcccgcgcg gcttcttcta ttggttcgag agcgcgagcc ccgagatgct cgagattctc
     1561 caggtcgagg cgatcgacaa gagcgtgaag aacgagcgcc tgaacgccac gccgcagaag
     1621 gatttcgcga cggagatcca gcggttttga agaacaggcg acgcccgttc attcaaggca
     1681 agagggcgga gcgatgagct cactcgtcat tcccgcgaag gcgggaatct aggccaagcg
     1741 ggtatttact ggatccccgc ctccgcgggg atgacttatg cggagcattc agatagccgc
     1801 cgcttttgtt gcctgcctgt cgggcctcgc tgcgcacgcg gcggaatcct ggcccgtccg
     1861 cccggtccgc ctgattgtca cctcgcctcc gggcggcgcc aacgacacgc aagcacgcgt
     1921 gctcggcgcg aagctcgccg agcagctcgg ccaggcggtg gtgatcgaca accgcggcgg
     1981 cggctcaggc atgatcgcag cggcaatcgt ggcgaagtcg gcgcccgacg gctacacgct
     2041 gctgttcggc accaactcca cctactcggt caatccctcg ctgtttccgc aggcgccgta
     2101 cgacacgctg cgcgatttcg cgccggtcgc ggtcaccgtg atgacgcccc acgtcctgct
     2161 ggtgcacccg tcgctgcccg cggcgacggt aaaagagctg attgcgctcg caagagcgca
     2221 gcccggccgg ctgaattacg cgtcctcggg caccggcagc gctttccacc tcggcatgga
     2281 actcctcaag accgcggcgg gcgtcgccat cgtgcacgtt ccgttcaagg gcagcgccct
     2341 gtcggcgaac gcgatgatcg cgggcgaggt ccagatgatg ctggtcggca tgccgaccgg
     2401 gatgccgctg gcgaaatcgg gtaaagcccg tgtgctcggg gtggcgacgc ccgcccgctc
     2461 ggtgctcgcg ccggagttgc cgacgatcgc cgaggcgggc gtgcccggct acggctacca
     2521 gagctggttc ggcgtggtcg cgccggccgg cacgccgcgg ccggtcatca cccggctgca
     2581 cgagggcttc gaccgcccgc
//
LOCUS       DBNGSG010000210         1940 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_236327, whole genome shotgun sequence.
ACCESSION   DBNGSG010000210 DBNGSG010000000
VERSION     DBNGSG010000210.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_236327"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..1026
                     /locus_tag="AC158Q_05600"
     CDS             115..1026
                     /locus_tag="AC158Q_05600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     polyprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HIR3339282.1"
                     /translation="MLGTLRKWGEFVKFSHTVFALPFALASMAIAARDTRGWPGLEKF
                     LLILVAMVCARTCAMAFNRIVDRKFDAANPRTAMRHLPTGEISLASAWTLWAMSAAGL
                     IVASWFLNPVCFYLSPVALAVVCFYSLTKRFTDYTHVFLGIALALAPLGAWLAVRGGF
                     SFEPLLGKTTGLIPIVLAVAVVLWLVGFDIIYALQDFEFDQRAGLRSLVVAWGPKVAL
                     TAAFLAHLFMWGLLAVFGLLSQFKIAYFVGLIFILGCLLVEHWLARKRSLKWINTAFF
                     KLNAVVSTMFLAVTVAEVAFPGFRVVR"
     gene            1069..>1940
                     /locus_tag="AC158Q_05605"
     CDS             1069..>1940
                     /locus_tag="AC158Q_05605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HIR3339283.1"
                     /translation="MYEKVVANERLTEADALRLFESKDLNAVGAIADFARARKTGNRA
                     SYIINRYINYSNYCILSCQFCSFARKKRDADGFELAVPQMVEKAREALKLGITELHVV
                     GGLHPSLPFSYYVDMVRSLKAVDARLQLKCFTAIEILHLAWLAKKPFPDVLAELKAAG
                     LDSLTGGGAEIFRPEVRSAIARGKESAEEYLDVHRAWHRMGGRSTCTMLFGHVESLAD
                     RVDHLRQLRALQDETGGFVGFVPLPYQPENNEIPVEHPPTGFDALRTLAVSRIYLDNF
                     AHITAYWVGLGLKLA"
BASE COUNT          308 a          592 c          633 g          407 t
ORIGIN      
        1 ctcggagcgg gggagaggga gtccgctggg ggcagtgggc ggatacgccc cgatgcgcgc
       61 ccgcaaaccg gatgcgcgtt gacgaacggc aggcagtggc ctgataacgg gcgggtgtta
      121 ggcacgcttc gcaagtgggg cgagttcgtc aaattctccc acaccgtgtt cgccctgccc
      181 ttcgcgctgg cctcgatggc catcgcggct cgggacactc gcggctggcc gggacttgaa
      241 aagttcctgc tcatcctcgt cgcgatggtc tgcgcgcgga cgtgcgcgat ggcgttcaac
      301 cggatcgtgg accgcaagtt cgacgcggcg aacccgcgca ccgccatgcg ccacctgccg
      361 accggggaga tctcgctggc cagcgcctgg acgctctggg ccatgagcgc tgccggactc
      421 atcgtcgcaa gctggttcct gaatccggtc tgtttctacc tttcgcccgt cgcgctggcg
      481 gtggtctgct tctattcgct gacgaaacgg ttcacggact atacccacgt gttccttggc
      541 atcgcgctgg cgttggcgcc gctgggagcg tggctggcgg tgagaggcgg gttttcgttt
      601 gagccgctgc tcggaaagac gaccgggctg atcccgattg tcctggcggt ggcggtcgtc
      661 ctctggctgg tcggcttcga catcatctac gcgttgcagg atttcgagtt cgaccagcgc
      721 gcggggctgc gctcgctggt cgtcgcgtgg gggccgaagg tcgcgctcac cgcggcgttt
      781 ttggcgcacc tgttcatgtg ggggctgctg gcagtgttcg ggttgcttag ccagttcaag
      841 attgcatact tcgtgggctt gatttttatc ctgggctgcc tgttggtgga gcattggctg
      901 gcgcgaaagc gaagcctgaa gtggatcaat accgccttct tcaagctcaa cgcggttgtc
      961 agcacgatgt tcctggccgt cacggtggcg gaagtggcgt tcccggggtt ccgggtcgta
     1021 cggtgatttc taatgaattt cttcctccaa cagagcgagc ttcgcgactt gtatgaaaag
     1081 gtcgtcgcca acgagcgcct caccgaggcg gacgcgttgc gcctgttcga gagcaaggac
     1141 ctcaacgccg tcggcgccat cgcggatttc gcgcgcgccc gcaagaccgg caatcgcgcc
     1201 agttacatca tcaaccgcta catcaactac tccaactact gcattctctc ctgccagttc
     1261 tgctccttcg cgcgcaagaa gcgcgacgcg gacggcttcg aattggccgt gccgcaaatg
     1321 gtcgagaagg cccgcgaggc gctcaaactc ggcattactg aactgcatgt cgtcggcggg
     1381 ttgcacccgt cgctgccgtt cagctactac gtggacatgg tgcgttcgct caaggcggtc
     1441 gacgcgcggc ttcagctgaa gtgcttcacg gcgattgaaa ttcttcacct cgcgtggttg
     1501 gcgaaaaaac cgttccccga cgtgctggct gaactgaagg ccgccgggct cgattcgctc
     1561 actggcggtg gcgcggagat tttccggccg gaagtccggt ccgccatcgc gcgcggcaag
     1621 gaaagcgccg aggaatacct cgacgtgcat cgcgcctggc atcgcatggg cgggcggagc
     1681 acctgcacca tgctgttcgg ccacgtcgaa tcgctcgctg accgcgtcga tcatttgcgc
     1741 cagcttcgtg cgcttcagga tgagacggga ggtttcgtcg gattcgttcc gctgccttat
     1801 cagccggaga acaacgagat tcccgtggag catccgccca cgggcttcga cgccctgcgc
     1861 acgctggccg tgagccgcat ctatttggac aacttcgccc atattacggc ctactgggtc
     1921 gggctgggac tgaaactggc
//
LOCUS       DBNGSG010000211         4634 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1595101, whole genome shotgun sequence.
ACCESSION   DBNGSG010000211 DBNGSG010000000
VERSION     DBNGSG010000211.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4634)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4634
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1595101"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="AC158Q_05610"
     CDS             complement(<1..636)
                     /locus_tag="AC158Q_05610"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3339284.1"
                     /translation="MAASVLKPPALPEFREDGLQTLFAHTEVRAFPRHKVIINEGGPS
                     NSVYLIRTGRVKVFLRSEDGKKVDINVLEPGDFFGEMALDEGPRSASVVTMVPTQLSV
                     IPQSVFRDFVAGDPDFAMRVILKLIMRTRGLLKNVKSLALLDVYGRVARTLMELATLE
                     NGNPVIKEKLSAQDIAYRIGASRESVSRILKDLVQGGYIRKDGMKITIARAL"
     gene            complement(765..1775)
                     /locus_tag="AC158Q_05615"
     CDS             complement(765..1775)
                     /locus_tag="AC158Q_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta/gamma crystallin family protein"
                     /protein_id="HIR3339285.1"
                     /translation="MKAMLMGAWVMAALAMSAQAAAQLTIYEREGFEGRSLTAKSRVS
                     DLRSMGFNDRASSAVVLGSQWEVCESAGFGGRCVVLRPGRYPSLGSMGLNDRVSSMRV
                     MSRSAGMDDRRYAPAPAAAQVIFYENAGFQGRHFITQKQVANFRRFGFNDRASSVVVI
                     GERWEVCENVQFKGRCVVLRPGRYSSLSAMGLNDRVSSVRAVGRGANIADNRYAPPPA
                     PVYDSRRRNNERLYEANVTSVRAVLGTPEQRCWVEREEIVQDQRNTSLSEAIAGILGY
                     QLADAQPEYQNVQRCATVPSRGRPDHWDVTYNFRGRDHRIQTTAPPGPTVTVNAQGEP
                     RV"
     gene            complement(2045..3103)
                     /locus_tag="AC158Q_05620"
     CDS             complement(2045..3103)
                     /locus_tag="AC158Q_05620"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR3339286.1"
                     /translation="MSTSRSDLPPVAADYFDGRTARAHKVTLMLAGGRLRVTGDGIER
                     EEPLHVLRVSEPMGAAPRLISFPGGAHCEVRDHAGLGALLESSGHVDSLVVRLQQRWR
                     AALASVVITVAALVAGYFWGLPALAEWIAFNIPEKLLVQMGSGTLQFLDRAALQPTKL
                     PAARQQELRRAFDRLVAPDGVKARHEIVFRRGGGVGANALALPDGTIVVTDELVGLAA
                     HDEETIAVLAHELGHLNRRHSLRMLIQGSIVAFVVAWYIGDVSNVATGLPTLLLQARY
                     SRDNEREADRFAAAMLKANGISPRRLGDMLMKLEAAHRARVADKDKRRQDDGAGGPFT
                     DYYSSHPATKERIEALAQ"
     gene            complement(3084..4148)
                     /locus_tag="AC158Q_05625"
     CDS             complement(3084..4148)
                     /locus_tag="AC158Q_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003263461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HIR3339287.1"
                     /translation="MDLISSGATAQPVQFTGKGSEYFRIWIVNLALTILTLGIYSAWA
                     KVRRLQYFYRNTSIAGNSFDFHGAPMAILKGRLIAVILLVAYNLAGNFNVFLGLAAFA
                     LLVAVLPWLVQRSFRFRLHNSSYRGLRFRFAGTVGGSYMAFLVWPLAAYFSLFVVAPM
                     AHREIKLYQHANSRFANVSAGFHAGYKPFYIIYLKALGMLLVGAIAMGIVMGVMGASG
                     LFSGDGSDPKQQGKIVAIIFAAIMAFYLLLFLFLGPWFSARMQNLVWNNTTLGPHAFS
                     SNVRARDLFVIYLTNFLAIILTLGLFKPFADIRIARYRLTHMALVAKTSLDEFLAEQE
                     QAVSALGEETADVLDVDISF"
     gene            4387..>4634
                     /locus_tag="AC158Q_05630"
     CDS             4387..>4634
                     /locus_tag="AC158Q_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3339288.1"
                     /translation="MIRRLAFLSVAAWAACAVSPALAQPYPHKPIRLLVGFAAGGGVD
                     KSARTVGQPLGESVGQTVVVDNRPGAAGNLAAGLVAKAT"
BASE COUNT          824 a         1520 c         1510 g          780 t
ORIGIN      
        1 cagggccctg gcgatcgtta ttttcatgcc gtccttccgg atatagccgc cctggaccag
       61 gtccttcagt atccggctga ccgactcccg cgacgcgccg atccggtagg cgatgtcctg
      121 cgcggacagt ttttccttga tgacgggatt gccgttctcc agcgtggcca gctccatcag
      181 cgtccgcgcc accctgccgt agacatccag cagggcgagg ctcttcacgt ttttcagcaa
      241 tccccgcgtc cgcatgatca gcttcaggat gacccgcatc gcgaagtcgg ggtcccccgc
      301 cacgaaatcc ctgaacacgg actgcggaat gacggatagc tgggtgggca ccatggtgac
      361 cacggaggcg gatctcgggc cttcgtcgag cgccatctcg ccaaagaaat cccccggctc
      421 cagcacgttg atgtccactt tcttgccgtc ttcgctgcgc aggaatactt tgaccctgcc
      481 cgtgcggatc aggtagacgg aattggacgg gccaccctcg ttgatgatga ccttgtgcct
      541 cgggaaagcc ctgacctccg tatgggcgaa cagggtctgc agcccgtcct cccggaactc
      601 cggaagggct gggggcttga gcacggaggc agccacgata cgttcgccgg gaccggtaaa
      661 ttctttatgt gccggtgcag ggcgggatca aggaggcgga acggtccacg ggcgggaggc
      721 tgtctctcgc cgtcaagctt tgccggcccg cggccttgca ggcgttatac ccggggttca
      781 ccctgggcgt tgacggtaac cgtcggcccg ggcggagcgg tggtctgtat gcgatgatcg
      841 cggccgcgga agttgtaggt gacatcccag tggtcgggcc ggccccggct gggcacggtg
      901 gcgcaacgct gaacgttctg atattccggc tgcgcgtcgg cgagctggta gccgagtatg
      961 ccggcaatcg cctcggacag actggtgttg cgttgatcct ggacgatttc ctcgcgctcg
     1021 acccagcatc gctgctcggg cgttcccagc acggcgcgaa ctgaagtgac attcgcttca
     1081 tacagccgct cgttgttccg ccggcgggag tcgtagaccg gggccggggg cggcgcgtag
     1141 cggttgtcag cgatgtttgc gcccctgcca actgcccgaa ccgatgaaac gcggtcgttc
     1201 aggcccatcg cggacagcga tgaataccgg cccggacgca ggacaacgca gcggcccttg
     1261 aactggacgt tttcacaaac ttcccagcgc tcgccgataa ccacaaccga cgaggcgcgg
     1321 tcattgaagc caaagcgccg gaagttggcg acctgtttct gcgtaatgaa atgccggccc
     1381 tgaaagccgg cattctcgta aaagatgacc tgcgcggccg cgggagcggg cgcatagcgc
     1441 ctgtcatcca tgcccgcgct cctgctcatg acgcgcatcg atgatacgcg gtcgttcagg
     1501 cccatcgagc ccagcgacgg ataccggccc ggacgcagga cgacgcagcg gccgccgaat
     1561 ccggcgcttt cacaaacctc ccactggctg ccgagaacca cggccgacga ggcccggtca
     1621 ttgaagccca tgctcctgag gtcgctgacc cggctcttcg cggtcaggga ccggccttca
     1681 aagccttcac gttcgtaaat ggtcaactgt gcggccgctt gcgcggacat ggccaatgcg
     1741 gccattaccc atgcgcccat caacattgct ttcatggaag tctcctgtat gaactatgca
     1801 tgccggcgtc cgggcgagcc aggcagccag ccagctaggc ggtcaatatg aagcaccgtc
     1861 gcaccagggt ctgtgcggag tcgaacataa ccggatcggg gggcaggggc cgctttttct
     1921 gcgggccgaa aagcccgcgc agcgctcggc agggggaggg ggcagggttt gcgatccgga
     1981 ctctcaggaa tagccgggat ccggccattg aggccaatgc caatccggcg ccaggcgccg
     2041 gcacttactg cgccaatgcc tcgatccgct ctttggtcgc cgggtgcgat gaatagtaat
     2101 cggtaaacgg gccgcccgcg ccgtcgtcct gccggcgttt atccttgtcc gccacccgag
     2161 cgcgatgggc cgcttcgagc ttcatcagca tgtcgcccag gcggcgcggc gatatgccgt
     2221 tggccttgag catggccgcg gcaaaacggt cggcctcgcg ctcgttgtcg cgcgaatagc
     2281 gggcctgcag cagcagggtc ggcaatcccg tggcgacgtt gctgacatcg ccgatgtacc
     2341 aggcgaccac gaaggcaacg attgagccct gtatcagcat gcgcaggctg tgccgccggt
     2401 tgagatgccc cagctcgtgg gcaaggaccg cgatggtttc ctcgtcgtgc gccgccaggc
     2461 ccaccagctc gtcggtgacg acgatggtgc cgtcgggaag cgccagtgca ttggcgccga
     2521 cgccgccccc ccggcggaat acgatttcgt gccgggcctt cactccatcc ggtgccacca
     2581 gcctgtcgaa cgcgcgacgc agctcctgct gccgtgccgc cggcagtttc gtgggctgca
     2641 gggccgcgcg gtccagaaac tgcagcgtgc cgctgcccat ctgcaccagc agcttttcgg
     2701 ggatgttgaa ggcgatccat tccgccagcg ccggcagtcc ccagaagtag cccgccacca
     2761 gcgccgccac cgtgatgacc accgacgcca gcgccgcgcg ccagcgctgc tgcagccgca
     2821 ccacaaggct gtccacgtgg ccgctcgact cgagcagcgc gccgagcccc gcgtgatcgc
     2881 gcacttcgca gtgcgcgccc ccgggaaacg agatcaggcg cggggcggca cccatcggct
     2941 ccgaaacccg cagcacgtgg agcggctcct cgcgctcgat gccgtcaccg gtgacccgca
     3001 ggcggccgcc cgccagcatc agcgtgactt tgtgcgcccg cgccgtccgt ccgtcaaagt
     3061 agtcggctgc gaccggcggc aggtcagaac gagatgtcga catcgagcac gtcggcggtt
     3121 tcctcgccca gcgcgctgac cgcctgctcc tgctcggcca ggaattcgtc caggctggtc
     3181 ttcgccacca gcgccatgtg ggtcagccgg tagcgggcga tgcggatgtc cgcaaacggc
     3241 ttgaacaggc ccagggtcag gatgatggcc aggaagttgg tgaggtagat cacgaacagg
     3301 tcgcgggcgc gcacgttgct gctgaacgca tgcggcccga gcgtggtgtt gttccagacc
     3361 aggttttgca tgcgcgccga gaaccacggg ccgagaaaca ggaacagcag caggtagaaa
     3421 gccatgatcg cggcgaaaat gatcgcgacg atcttcccct gttgcttcgg gtcgctgccg
     3481 tcgccgctga acagcccgga cgcgcccatc acgcccatca ctatgcccat cgcgattgcc
     3541 ccgacgagca gcatgccgag cgccttgagg tagatgatgt agaacggctt gtaaccggcg
     3601 tggaagccgg ccgaaacgtt ggcaaaccgg ctgttggcgt gctggtagag cttgatttcg
     3661 cggtgcgcca tcggcgcgac cacgaacaac gagaagtagg cggcgagggg ccacaccagg
     3721 aacgccatgt aggacccgcc caccgttccg gcgaagcgaa agcgcaggcc gcgatagctg
     3781 ctgttgtgca gcctgaagcg aaacgaccgc tgcaccagcc agggcaggac cgcgaccagg
     3841 agggcgaaag cggcgaggcc caggaacacg ttgaaattgc cggccaggtt gtacgcgacc
     3901 agcagaatca ccgcgatcag gcgtcccttc agtatcgcca tgggcgcgcc gtgaaagtcg
     3961 aagctgttgc cggcgatgga ggtgttgcgg tagaaatact gcaggcggcg caccttggcc
     4021 cacgccgaat agatgccgag cgtgaggatc gtcagcgcga gattgacgat ccagatccgg
     4081 aaatattccg agcccttgcc ggtgaactga acaggctgcg ccgtcgcccc gctcgatatc
     4141 aggtccatgg tcccccctgt tagatgccct ggactgctgt cgcacgaccg gggcggctac
     4201 cccggattca ttacatgcca acgtcttgtg ggagatgata cgcgatcccc gccgcaccgg
     4261 cattcgcgcc cgcgctgctc gcgccacgcc cgctcccggc gacagcgcag agcactggca
     4321 tgacaggcgg gctcagtgga caatggggcc ggacccagtc agaaaaacac attacctgga
     4381 ggattcatga tcagaaggct tgctttcctg agcgtcgccg catgggcggc ctgcgccgtg
     4441 tcaccggccc tggcgcagcc ctatccccac aagccgatcc ggctgctggt cggatttgcc
     4501 gccggcggcg gcgtcgacaa gtccgcgcga acggtcggcc agccgctcgg cgagtcggtg
     4561 ggccagacgg tcgtcgtgga caaccgtccg ggcgcggcgg gcaatctcgc cgccggcctg
     4621 gtggcgaagg cgac
//
LOCUS       DBNGSG010000212         9885 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1254872, whole genome shotgun sequence.
ACCESSION   DBNGSG010000212 DBNGSG010000000
VERSION     DBNGSG010000212.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9885)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9885)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9885
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1254872"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..919)
                     /locus_tag="AC158Q_05635"
     CDS             complement(179..919)
                     /locus_tag="AC158Q_05635"
                     /inference="COORDINATES: protein motif:HMM:NF024581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3339289.1"
                     /translation="MPIHEQSLIRPENLTTHDNLVLDGVDVSGHWSTFITPRVVTDYN
                     ERIEEEISALPGGEFIHRCWQCGSCTNSCTVNAVNPDFNPRYWIYLIRTGMEAELLRD
                     KDIIWQCVSCNKCTYACPRDVFPEGVMKATAHWLELKGHTPKSKSMHFDEEFTEQVVE
                     TGRIEEGRIIRNFFARTGQPLLQEWLVEMARRVARRLPMKLALLMGLSALIRPRTRGW
                     GRARDAIGEYLSEQEALQRKALGLKEKA"
     gene            complement(934..1773)
                     /locus_tag="AC158Q_05640"
     CDS             complement(934..1773)
                     /locus_tag="AC158Q_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339290.1"
                     /translation="MTQAIVRSALERPHLDLSGPRLARALEVLVSAAEDQGGIERYVE
                     ALRVKTALFQAALASADAIDPGTFKPLFAHLPTVRRRVGPWLVPPRIGELRARIAALL
                     DGREDTATTDERIAAFCAAFPDDREHRWVRDLAAEILHNADPERYPLMCRWVWDVRAN
                     TGALREMWHADDLDRLLIDVPDRYGTFIMLREELAQYLARNGFFRDVVHYVDLLTAQV
                     YAGYISEQGDSYLRADFANPEDPLLHTRRLLGLDGIGSGGRTRLKAVDGTACVVDDVK
                     RLD"
     gene            complement(1784..2836)
                     /locus_tag="AC158Q_05645"
     CDS             complement(1784..2836)
                     /locus_tag="AC158Q_05645"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3339291.1"
                     /translation="MTKSVLIVGGGPAGLAAAHALATVGQRSIVVEKESKLGGAPILS
                     GYARLVPSGEWARDAIGGMVNRVKESPLVTVHTGSTVTSFSGSAGAFQAGLSGGETLE
                     AGAALLCTGFTHFDSVNKPEWGFGTFPDVVTTTQVEQMISSGKGVRCPSDGRKPQRVA
                     ILLCVGSRDRQIGREWCSKICCTVSSNLAMEIREELPDCHVYIYYMDIRTFGLYETRY
                     YWRSQEEFKVKYIKARIAEVTSDGHKLIIKGEDTLVKRPITIPFDLVVHAIGMDPNTG
                     NRTLAKTFGVELEPHGFIGKGNAYGALGETSRPGVFVAGAATGPETIDDSIAQAKAAA
                     MAAFALTNRPEQVATA"
     gene            complement(2833..4188)
                     /locus_tag="AC158Q_05650"
     CDS             complement(2833..4188)
                     /locus_tag="AC158Q_05650"
                     /inference="COORDINATES: protein motif:HMM:NF014774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HIR3339292.1"
                     /translation="MESKLNCGERFTGHGAEWRDANLSPEDARMATAWVEMKVDRRAM
                     LTQKDRVEDVRDIMWQLEKDGEIVVHRVTDAHEPVMVKTLYGWDKKIPTRRLWHHKSC
                     GQCGNTPGYPASLLWLMNRLGVEYLDETDQTSCTAWNYHGSGIGNLESLAAVFLRNFH
                     QAYVSARAQGLPDAYFYPLVHCGTSFGNYKEVRGYLLRSAKLRERVRQILGKLGRLVD
                     GKLLIPEEVVHYSEWVHVMRDRIAQLQEIDCSRVRATIHPACHVYTMVPEDAIYDDAI
                     LGGNRVAVSTGILEALGAQVIDYSTWYDCCGFGFRHIISEREFTRSFAIDRKIRVAVE
                     EAHSDVMIGHDTGCITTLDKNQWISKSGARPVELPVLADCQFAALVCGAHPYKIVQSH
                     WHASPVETLMAKLGIDWQAKKAEFMAYLEEVKAGKPENLYDPRRMITSGPGFVPMAKR
                     P"
     gene            complement(4199..4954)
                     /locus_tag="AC158Q_05655"
     CDS             complement(4199..4954)
                     /locus_tag="AC158Q_05655"
                     /inference="COORDINATES: protein motif:HMM:NF024581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3339293.1"
                     /translation="MTHATTTRVQARIGDPLSEQPPVPREKLESIFKDIQSDLRFDHE
                     LNGCLNCGICTATCPAAHYYDFSPREIVQLLWTENLEGIYDAMQEKIWACAQCYTCAA
                     RCPFGNSPGGLVMLMRETAIKHEMDSAKNVLRPFSRVMLKLISTGNQLSPDMINPDHF
                     ADWGPNISKIDAPLKLLRRAIPMPTLNTTATAWEVNLKTSVELYTIWEETGVLKQLEG
                     IDENLFDVITDIMDEKREDWKDFLADQADTKDD"
     gene            complement(4968..5345)
                     /locus_tag="AC158Q_05660"
     CDS             complement(4968..5345)
                     /locus_tag="AC158Q_05660"
                     /inference="COORDINATES: protein motif:HMM:NF014672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HIR3339294.1"
                     /translation="MAGKLVIVMANTDPRNGEELGAPIFQATVAAAMDYEVDVICTAT
                     SGRLMKKGVAEKLFVKEGSPKSVYDFIKDAHEAGVKFYCCSPNLDLFEMKEDDLIPEC
                     SGIVGGAHLIEEVMEGDCKVLTY"
     gene            complement(5426..6124)
                     /locus_tag="AC158Q_05665"
     CDS             complement(5426..6124)
                     /locus_tag="AC158Q_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339295.1"
                     /translation="MKTVCVVQHTSAEYLGLIEDHLEGRGIRFRYYRPFTESGRLPDR
                     DAVREGLVLLGGGVWGAAGTRDVPTLDAEVRLAHAALMFGSPVIGIGLGAQILAIAAG
                     GRAAPSPLAFEVITARRADEAALNRYLPAQYPLALYMRDWPEPPAYARMLATDPAGRP
                     VLWQIGERAFGFAGHPGLKVAMVEDLIMEFDEAPRDPAPALAQLRAAQRAMEDALVPV
                     MTGLVQLTGWMQPD"
     gene            complement(6154..7221)
                     /locus_tag="AC158Q_05670"
     CDS             complement(6154..7221)
                     /locus_tag="AC158Q_05670"
                     /inference="COORDINATES: protein motif:HMM:NF046470.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl protein ligase domain-containing protein"
                     /protein_id="HIR3339296.1"
                     /translation="MAAVFRVIDTGVRDGRRQIAFDQALIEAHKAGKIPDSIRFLRFP
                     PTALVGRHQVLSREIDLAHCRTNGVGVARRITGGGAIYFDEGQLGWELVLRRSTLGIS
                     SLADFTRRICEAAAAGLSRLGVDAKYRPRNDIEVGGRKVSGTGGFFDGGTLFFQGTVL
                     VDMNPADMLAALKVPRAKLEKHALDSAAQRVATLKELLGAAPPLEAVRDALLAGFSER
                     LGISTVPGTITEFEETLAARLHDEEIGNDAFVAEIDEPAADGETLSASLTCAGGTIEA
                     HIRLEGARRDRIREALITGDFFVTPPRTVFDLEAALRNVEVADAGAVVERFFAQAGVG
                     LLTAAPADFRKAVEDAIAQAR"
     gene            complement(7233..8366)
                     /locus_tag="AC158Q_05675"
     CDS             complement(7233..8366)
                     /locus_tag="AC158Q_05675"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR3339297.1"
                     /translation="MKNGARVKVNNVAPQGVYLPNYNILTPHMRSPEYVQMSTAAAIT
                     LGVMNGRMHRCSCTRCLNLLLTYPEGCRANCAYCGLARHREAERDYADRNFIRVDWPA
                     VPLAEVIDIVARDAAASPFHRMCISMITHPRSDADTVTVLKQWTDRIEPSAIPVSILS
                     NPTTMSRADVARLHDLGADIFTVALDAATPEIFDRTRGKGVQSPHSWAKYWEILMAAR
                     DIFGPQKFGAHIIVGMGETEHDVLALVQRLVDLGGHSHLFCFFPEKGSLMDHLPATPR
                     DQWRRVQLARYLIDYRGVRVDNMRFDGQGRVMDFGLPQGELDTIVGEGVAFRTSGCPG
                     KFRDDVSACDRPYGDSPPSNIASFPFQPGRTDLRRIRRQLRPV"
     gene            complement(8508..9575)
                     /locus_tag="AC158Q_05680"
     CDS             complement(8508..9575)
                     /locus_tag="AC158Q_05680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HIR3339298.1"
                     /translation="MAAERFDVVVIGLGPAGACAAAEAARRGCRVLAVERKREPGIPV
                     QCAEFVPAMIGVEARGFADSVRQSIRAMMTFVEGGAPDVKSDFSGYMLDRAVFDAGLA
                     AAAARAGADCRFGTRVRAISPDGCVELADGQRLAAAVIVGADGPRSAVGRRIGHVNRK
                     LLETRQLTVPLRNPHDATDIFLSAGIPGGYGWLFPKGGIAHVGAGVDAAQRARLRNIV
                     SGLHATLVENGRVGAGILGRTGGAIPAGGMLKPWGTAGDTLALLAGDAAGLTNPVTGA
                     GIPAAVYSGRLAGETAAAWIAGNREAGENYEGELRGVFEQALERAVLRRRELSLHSRG
                     NALPGRAAWRRGWIAYPEYWA"
     gene            complement(9575..>9885)
                     /locus_tag="AC158Q_05685"
     CDS             complement(9575..>9885)
                     /locus_tag="AC158Q_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339299.1"
                     /translation="FYTKPGMFQTPGTGDVGRVFLDARRRLPGRPVLLGCARPAGLHR
                     RVTDAYAVMAGLDGIAFPAEGTLAVATAIGRPAVQEHACCSIRTGQSIKLHRRESLAT
                     "
BASE COUNT         1510 a         3299 c         3385 g         1691 t
ORIGIN      
        1 gtctggacct tcgggtcctt gttcttcacc tccatcgcgc cgcagcagtt ccagttcttc
       61 acctagtcga gctcgagccc gagcgccttc cccacttccc gggtggactc gttgtaggcg
      121 tggccggtgc cctcgagcgc gcagcccggg tagtaggcga cgcgcttgta ttcggtcttc
      181 atgctttctc cttgaggccg agcgccttgc gctgcagcgc ttcctgctct gagaggtact
      241 cgccaatcgc gtcgcgcgct cttccccagc cgcgggtgcg cggccgtatc agggcggaga
      301 gccccatcag cagagcgagc ttcatcggca ggcggcgcgc cacgcgcctc gccatctcga
      361 ccagccactc ctgcagcagc ggctggccgg tgcgcgcgaa gaagttgcgg atgatgcgac
      421 cttcctcgat gcggcccgtc tcgacgacct gctcggtgaa ttcttcgtcg aagtgcatcg
      481 acttcgactt cggcgtgtgc cccttcagtt cgagccagtg cgccgtcgcc ttcatcactc
      541 cctcggggaa aacgtcgcgc gggcaggcgt aagtgcactt gttgcacgac acgcactgcc
      601 agatgatgtc cttgtcgcgc agcagctcgg cttccatgcc cgtgcggatc agatagatcc
      661 agtagcgcgg gttgaagtcc gggttgacgg cgttgacggt gcaggaattg gtgcacgagc
      721 cgcattgcca gcagcggtgg atgaactcgc cgcccggcag cgcggaaatt tcctcctcga
      781 tgcgctcgtt gtagtcggtg acgacgcgcg gcgtgatgaa ggtgctccag tgaccggaga
      841 cgtcgacccc gtccagcacg agattgtcgt gcgtcgtcag gttctcgggg cggatcagcg
      901 attgctcgtg gatgggcatg cgcgggacgt ccttcagtcg agtcgcttca cgtcgtcgac
      961 cacgcaggcc gtgccgtcga ccgctttcag ccgggtccgg cccccgctgc cgatgccatc
     1021 cagccccagc aggcgacggg tgtgcagcag cgggtcctcc gggttggcga aatcggcgcg
     1081 caggtagctg tcgccctgct cgctgatgta gccggcgtaa acctgggcgg tcaggaggtc
     1141 gacgtaatgc acgacgtcgc ggaagaaccc gttccttgcg aggtactgcg ccagctcttc
     1201 gcgcagcatg atgaaggtgc cgtaacggtc cggcacgtcg atcagcagcc ggtcgaggtc
     1261 gtccgcgtgc cacatttcgc gcagcgcacc ggtattggcg cgcacgtccc acacccaccg
     1321 gcacatcagg ggatagcgct ccgggtcggc gttgtgcaga atctcggcgg caaggtcgcg
     1381 cacccagcgg tgctcgcggt cgtcgggaaa ggcggcgcag aaggcggcaa ttctctcatc
     1441 agtggtggcc gtgtcctcgc ggccgtcgag cagagcggcg atgcgcgcgc gcagttcgcc
     1501 gatgcgcggc gggacgagcc agggacccac gcgcctgcgc acggttggca ggtgcgcgaa
     1561 cagcggcttg aacgtgccgg ggtcgatggc atccgcggag gcgagcgccg cctggaacag
     1621 ggccgtcttc acgcgcaggg cctcgacgta gcgctcgatg ccgccctgat cctcggccgc
     1681 ggagacgagg acctccagcg cgcgcgcgag ccgggggccg gagagatcga ggtgcggccg
     1741 ttccagcgcc gatcggacta tcgcctgcgt catggcgcga tcttcaggct gtcgccacct
     1801 gttccgggcg gttcgtcagg gcgaaagcag ccatcgcggc ggcttttgcc tgcgcgatcg
     1861 agtcgtcgat ggtttccggc ccggtggccg cgcccgcgac gaacaccccc gggcgcgagg
     1921 tctcgcccag cgcgccgtag gcgttgccct tgccgatgaa gccgtgcggt tccagctcga
     1981 cgccgaacgt cttcgccagc gtccggttgc cggtgttggg atccatgccg atggcgtgca
     2041 cgaccaggtc gaacgggatg gtgatcgggc gcttcaccag cgtgtcctcg cccttgatga
     2101 tgagcttgtg gccgtcggag gtgacctccg cgatgcgcgc cttgatgtac ttgaccttga
     2161 actcttcctg gctgcgccag tagtagcgcg tctcgtagag gccgaacgtg cggatgtcca
     2221 tgtaatagat gtagacatgg cagtcgggca gctcctcgcg gatctccatc gccagattgg
     2281 aggacacggt gcaacagatc ttggagcacc actcgcgtcc gatctgccgg tcgcgcgagc
     2341 cgacgcacag caggatggcc acgcgctgcg gttttcgtcc gtccgacggg cagcgcacgc
     2401 ccttgccgga ggagatcatc tgctcgacct gggtggtggt cacgacgtcg ggaaaggtgc
     2461 cgaagcccca ttccggcttg ttgaccgagt cgaagtgcgt gaacccggtg cagagcaggg
     2521 cggcgccggc ttcgagcgtc tcgccgccgg agagccccgc ctggaacgcg ccggccgagc
     2581 cggagaacga cgtcaccgtg ctgcccgtgt gcacggtgac cagcgggctt tccttcacgc
     2641 ggttcaccat gccgccgatc gcgtcgcgcg cccattcgcc ggacggcacc agccgggcat
     2701 agcccgacag gatcggcgcg ccgccgagtt tcgattcctt ttcgacaact atggatcgct
     2761 ggccgacggt ggcgagcgcg tgggccgcgg caagaccggc cggcccgccg ccaacgatga
     2821 gaacggactt ggtcatggcc gtttcgccat cgggacaaac ccggggcccg aggtgatcat
     2881 acggcgcggg tcgtagaggt tttccggttt gccggccttg acctcctcca ggtacgccat
     2941 gaactcggcc ttcttcgcct gccagtcgat gccgagcttg gccatcagcg tttcgaccgg
     3001 cgaagcatgc caatgcgact gcacgatctt gtacgggtgc gcgccgcaga ccagcgccgc
     3061 gaactggcag tcggcgagca cgggcagctc cacggggcgc gcgccggatt tgctgatcca
     3121 ctggttcttg tcgagcgtcg tgatgcagcc ggtgtcgtgg ccgatcatca cgtcggagtg
     3181 cgcctcctcg acggcgacgc ggatcttgcg gtcgattgca aacgagcggg tgaactcgcg
     3241 ctcggaaatg atgtggcgga agccgaagcc gcagcagtcg taccacgtcg agtagtcgat
     3301 cacctgagcg ccgagtgcct ccaatattcc ggtcgacacc gccacgcggt tgccgccgag
     3361 gatggcgtcg tcgtagatcg cgtcctccgg caccatcgtg tagacgtggc aggcggggtg
     3421 gatcgtcgcg cggacgcggc tgcagtcgat ctcctgcagc tgcgcgatgc gatcgcgcat
     3481 cacgtgcacc cactccgagt aatggacgac ttcctcgggg atcagcagct tgccgtccac
     3541 cagccggccc agcttgccga ggatctggcg cacgcgctcg cgcagcttcg ccgagcgcag
     3601 caggtagccg cgcacttcct tgtagttgcc gaacgaggtg ccgcagtgca cgagcggata
     3661 gaagtaggcg tccggcagcc cctgcgcgcg ggcggagacg taagcctggt ggaagttgcg
     3721 caggaacacc gcggccaggc tctcgaggtt gccgatgccc gagccgtgat aattccacgc
     3781 ggtgcaggag gtctggtcgg tctcgtcgag gtattcgacg ccgagccggt tcatgagcca
     3841 cagcagcgag gcggggtagc cgggggtatt gccgcactgg ccgcacgact tgtggtgcca
     3901 caggcgccgc gtcgggatct tcttgtccca gccgtagagc gtcttgacca tgaccggctc
     3961 gtgggcgtcc gtcaccctgt gcacgacgat ctcgccgtcc ttctccagct gccacatgat
     4021 gtcgcgcacg tcctccacgc ggtctttctg cgtgagcatc gccctgcggt cgactttcat
     4081 ctcgacccac gcggttgcca tgcgcgcgtc ctcgggcgaa aggttcgcgt cgcgccattc
     4141 ggcgccgtgg ccggtgaagc gctcgccgca gttcagcttg gattccatgt ccgcctcctc
     4201 agtcatcttt cgtgtctgcc tggtccgcga ggaagtcttt ccagtcctcg cgcttctcat
     4261 ccatgatgtc ggtgattacg tcgaacaaat tctcgtcgat cccctcgagc tgcttcagca
     4321 cgccggtctc ctcccagatc gtgtagagct cgaccgaggt cttgagattg acttcccacg
     4381 cggtggccgt ggtgttcaac gtcggcatcg ggatcgcgcg gcgcaggagc ttcagcggcg
     4441 cgtcgatctt ggagatattg gggccccagt ccgcgaagtg atcgggattg atcatgtccg
     4501 gggagagctg gttgccggtg gaaatcagct tcagcatcac gcgcgagaac ggccgcagca
     4561 cgtttttcgc ggaatccatc tcgtgcttga tcgcggtctc gcgcatcagc atgaccaggc
     4621 cgccgggcga gttgccgaac gggcagcgcg cagcgcaggt gtagcactgg gcgcaggccc
     4681 agatcttctc ctgcatggcg tcgtaaatgc cctcgaggtt ctccgtccac aacagctgga
     4741 cgatctcgcg cgggctgaag tcgtaatagt gcgcggccgg gcaggtcgcg gtgcagatgc
     4801 cgcagttgag gcagccgttc agctcgtggt cgaagcgcag gtcggactgg atgtccttga
     4861 agatgctctc cagcttctct cgcggtactg gcggttgctc cgagagggga tcgccgatgc
     4921 gggcctgcac ccgcgtggtc gttgcgtggg tcatctccgt ggcgccttca gtaagtcagc
     4981 accttgcaat cgccctccat cacctcctcg atgaggtggg caccgccgac gatgccggag
     5041 cattcgggaa tgaggtcgtc ttccttcatc tcgaagagat ccaggttggg tgagcagcag
     5101 tagaacttga cgccggcctc gtgcgcgtcc ttgatgaagt cgtagaccga tttcggcgag
     5161 ccttccttga cgaagagctt ctcggccacg ccttttttca tcagccgccc ggaggtggcg
     5221 gtgcagatga cgtcgacctc gtagtccatg gcggcggcga cggtcgcctg gaagatcggc
     5281 gcgcccagct cttcgccgtt gcgcgggtcc gtattggcca tcacgatgac cagcttgccg
     5341 gccatgacgc ctctccgcct ctcgttgtgg ctgccgtgca aaacactata ttaggacttt
     5401 ctaatagatg caagcccgca gcggctcagt ccggctgcat ccatcccgtc agctgcacga
     5461 ggccggtcat gaccggcacc agcgcgtctt ccatggcgcg ctgcgccgcg cgcagttgcg
     5521 ccagcgccgg ggcggggtcc cgcggcgctt cgtcgaactc catgatcagg tcctcgacca
     5581 tcgccacctt gagccccgga tgccccgcga acccgaacgc ccgctcgccg atttgccaca
     5641 ggacggggcg gcccgcggga tccgtcgcca gcatccgggc gtacgcgggc ggctccggcc
     5701 agtcgcgcat gtagagcgca agcggatatt gcgccgggag atacctgttc aaggcggcct
     5761 cgtcggcacg acgcgcggtg atcacctcga acgcgagcgg ggacggcgcg gcgcgcccgc
     5821 ccgctgcaat ggcgagtatc tgcgcgccca gcccgatgcc gatgacgggg ctgccgaaca
     5881 tgagcgcggc atgggccagc cggacttccg cgtccagcgt cggcacgtcg cgcgtgcccg
     5941 cggcgcccca caccccgcct cccagcagca ccagcccttc gcggaccgcg tcgcgatcag
     6001 gcagccgccc gctttcggtg aatgggcggt aataccgaaa gcggatgccg cggccctcga
     6061 gatggtcctc gatcaggccg agatactcgg cggaggtgtg ctggacgacg cagaccgttt
     6121 tcatgcgcgt ctccatgcgc gtcttcatgc cgctcagcgg gcctgcgcga tcgcgtcttc
     6181 cacggccttg cggaagtccg ccggtgctgc cgtgagcagg ccgaccccgg cttgcgcgaa
     6241 gaaacgctcg acgacggcgc ccgcgtcggc aacttcgaca ttgcgcagcg ccgcttccag
     6301 gtcgaagacg gtgcgcggcg gcgtgacaaa gaagtctccc gtgatgagcg cctcacggat
     6361 gcggtcgcgc cgcgcgccct cgagccggat gtgcgcttcg atcgtgccgc cggcgcaggt
     6421 caggctggcc gaaagtgtct cgccgtccgc ggcaggctcg tcgatctcgg cgacgaaggc
     6481 gtcgttgccg atctcctcgt cgtggagccg cgcggcgagg gtctcctcga attcggtgat
     6541 cgtgccgggg accgtcgaaa tgccgagccg ctcggagaaa cccgccagca gcgcgtccct
     6601 gaccgcttcc agcggcggcg cggcgccgag cagttccttc agcgtcgcga cccgctgtgc
     6661 cgccgaatcg agcgcgtgct tctcgagctt ggcgcggggc accttgagtg cggcgagcat
     6721 gtcggcggga ttcatgtcca ccagcacagt gccctgaaag aacagcgtgc cgccgtcgaa
     6781 gaaaccgccg gtgccgctta ccttgcgccc gcccacctcg atgtcgttgc gcggccggta
     6841 tttcgcatcg acgccgaggc gggagaggcc cgccgcagcc gcctcgcaga tacggcgcgt
     6901 gaaatccgcg agcgagctga tgccgagggt cgagcggcgc aggaccaatt cccatcccag
     6961 ctgaccctcg tcgaaataga tcgcgccgcc gccggtgatg cggcgcgcga ctccgacgcc
     7021 attggtgcgg cagtgggcga gatcgatttc gcgcgacagc acctggtggc ggccgaccag
     7081 cgcggtgggc gggaagcgca ggaagcggat ggaatcgggg attttcccgg ccttgtgcgc
     7141 ctcgatcagg gcctggtcga acgcgatctg ccggcgccca tcgcgcacgc cggtgtcgat
     7201 tacacggaag accgccgcca tgccgctttc cttcagaccg gccgcaactg gcgccggatc
     7261 ctgcgcaggt cggtcctgcc gggctggaac gggaagctgg cgatgttgct gggaggcgag
     7321 tcgccgtagg ggcgatcgca cgcggacacg tcgtcgcgga acttgccggg gcagccggag
     7381 gtgcggaacg cgacgccctc gccgacaatg gtgtcgagct cgccttgcgg caggccgaaa
     7441 tccatcacgc gcccttgccc gtcgaagcgc atgttatcga ctcgcacgcc gcggtagtcg
     7501 atcaggtagc gggcaagctg cacgcgacgc cactggtcgc gcggcgtcgc cggcaggtgg
     7561 tccatcagcg agcccttctc ggggaagaag cagaacaggt ggctgtggcc gcccaggtcc
     7621 accaggcgct gcaccagcgc gagcacgtcg tgctcggtct cgcccatgcc gacgatgatg
     7681 tgcgcgccga acttctgcgg accgaagata tcccgcgcag ccatgaggat ctcccagtac
     7741 ttggcccacg agtgcggcga ctgcacaccc ttgccgcgcg tgcggtcgaa gatttccggc
     7801 gtcgcggcgt cgagcgccac ggtgaagatg tccgcgccga ggtcatgcag acgcgcgacg
     7861 tccgcgcgtg acatggtcgt cgggttcgac aggatcgaga ccgggatcgc cgagggctcg
     7921 atgcggtcgg tccactgctt cagcacggtc acggtgtccg cgtcggagcg ggggtgagtg
     7981 atcatcgaga tgcacattcg gtggaacggg ctcgcggcgg cgtcgcgcgc gacgatgtcg
     8041 atgacttccg ccaggggcac cgccggccag tcgacgcgga tgaaattgcg atcggcatag
     8101 tcgcgctcgg cctcgcgatg acgggcgagt ccgcagtacg cacagttggc gcggcagccc
     8161 tccgggtacg tgagcaggag attcaggcag cgcgtgcacg agcagcggtg catgcggccg
     8221 ttcatgacgc cgagcgtgat cgccgccgcc gtcgacatct gcacgtactc cggcgagcgc
     8281 atgtgcggcg tcagaatgtt gtaattgggc aggtagacgc cctgcggcgc cacgttgttg
     8341 accttgaccc gtgccccgtt cttcatctcg cgcggagtag cggcgggcgt ccgcggctgc
     8401 atcacggcag cagcgttaat gtccggcgca gttgagtcag ctacagtgga cgcggtttca
     8461 gggcgatagc agctcatggt ccgttccttt gctgtttcgg gtgagcctca ggcccagtac
     8521 tccgggtagg cgatccagcc gcgacgccac gccgcgcggc cggggagggc gttcccgcgc
     8581 gaatgcagcg agagttcgcg cctgcgcagc acggccctct cgagcgcttg ctcgaacacg
     8641 ccgcgcagct cgccttcata gttttctccg gcctcgcgat tgcccgcgat ccacgccgcc
     8701 gccgtctcgc ccgcgagccg gccggaatag acggcagcgg ggatgccggc gccagtcacc
     8761 gggttggtca agccggcggc gtctcccgcc agcagcgcga gcgtgtcgcc tgccgtgccc
     8821 cagggtttca acatgccgcc ggcgggaatc gcgccgccgg tgcggccgag gatgcccgcg
     8881 ccgacgcgcc cgttttcgac cagcgtcgcg tgcaaccctg agacgatgtt cctgagtcgc
     8941 gcccgctgag cggcgtcgac tccggcgccc acgtgcgcga tcccgccctt ggggaacagc
     9001 cagccgtagc cgccgggaat gccggccgag aggaagatat cggtggcgtc gtgcggattt
     9061 cggagcggca cggtgagctg ccgggtttcc agcaacttcc ggttgacgtg gccgatgcgg
     9121 cgcccgacgg cggagcgggg gccgtcagcg ccgacgatca ccgcagccgc gagacgctgt
     9181 ccgtcggcga gctcgacgca tccgtcgggc gagatcgcgc gcacgcgagt cccgaagcgg
     9241 caatccgcgc ccgcgcgggc cgccgccgcg gccagccctg cgtcgaacac ggcgcgatcg
     9301 agcatgtagc cggagaagtc gctcttcacg tccggcgcgc cgccctcgac gaacgtcatc
     9361 atggcgcgga tggactggcg caccgaatcc gcgaagccgc gggcctcgac gccgatcatc
     9421 gccggcacga actccgcgca ctgcacggga atgccgggct cgcgcttgcg ctcgaccgcg
     9481 aggacgcggc agccgcgccg cgccgcttcc gcggccgcgc aggcgccggc gggcccgagg
     9541 ccgatgacca cgacgtcgaa gcgttcggca gccatcacgt cgctagcgat tcgcgccggt
     9601 gcagcttgat ggattggccg gtgcggatgg agcagcaggc gtgctcctgc accgccgggc
     9661 ggccgatggc cgtggcgacc gccagcgtgc cttcggccgg gaaggcgatg ccgtcgagcc
     9721 ccgccatgac ggcgtaggcg tcggtcaccc ggcggtgcag gccggctggg cgtgcgcagc
     9781 ccagcagcac cgggcgccca ggcaggcgcc gcctcgcgtc gagaaacacc cggcccacgt
     9841 cgcccgtccc gggcgtctga aacatgcccg gcttcgtata gaagg
//
LOCUS       DBNGSG010000213         5503 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1765630, whole genome shotgun sequence.
ACCESSION   DBNGSG010000213 DBNGSG010000000
VERSION     DBNGSG010000213.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5503)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5503)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5503
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1765630"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(240..1073)
                     /locus_tag="AC158Q_05690"
     CDS             complement(240..1073)
                     /locus_tag="AC158Q_05690"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR3339300.1"
                     /translation="MDDLDFTNANRQASSEVYKPSIAKMCFEALGRVEVMSQGGTFFT
                     ENQASDKMYLLVEGEVGLIRGKKVLDTVKAGEVFGEMAAISRQPRSATAAAKTACRTL
                     SLDAGQFQSALQRTPEFAIMLMNIIINRLRLTAALVSRNLPGAAQVQELRVFDPPLLA
                     ALVEHLQHRPPAHAPLNKVIMKEGEGGVFCYIVLEGQVAISIKSKVVNRVGAGGVFGE
                     MALIDQSPRAATATAETDCELLSITRNDFLGLVKSNPAFAVALLKSLADRLRYMTSHH
                     K"
     gene            complement(1083..2450)
                     /gene="xseA"
                     /locus_tag="AC158Q_05695"
     CDS             complement(1083..2450)
                     /gene="xseA"
                     /locus_tag="AC158Q_05695"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HIR3339301.1"
                     /translation="MLERQPGLPLPQIISVAELNRLTRELLESNLPLLWVAGEISNFR
                     RYDSGHCYFTLKDAQAQVDCVMFRGRAQALGWQPQDGVKAEVRALPTLYEARGKFQLN
                     VEAMRRAGLGALYEAFERLKAKLEKEGLFDPARKRPLPRFPRTVGVVTSPQAAALRDV
                     LTTLKRRMPGLPVILYPTPVQGEGAGDRIAAAIALAGARRECDVLVVCRGGGSIEDLW
                     AFNEEAVARAILASPVPVVTGIGHETDFTIADFVADARAPTPTGAAALATPGRDELAR
                     LLLQQAHRLLRVFRRGLEDRMQKLDFLSRRLVHPGERIRNQVSELRHLATRLAGAWER
                     AREDLGWQVRELGLRLKSGAPDVGGRIQDAAALARRLRDTGRRRLERAAALLARLDAN
                     LKHLNPQSVLERGYSITETAGGVIVRDAGRLAIGEDVTITFAKGRAGARVVRKDAAGD
                     REPER"
     gene            2923..3528
                     /locus_tag="AC158Q_05700"
     CDS             2923..3528
                     /locus_tag="AC158Q_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR3339302.1"
                     /translation="MFAIIEAAGWPIWFLIVASVIALAIIGERWWSLRESVVMPRNLL
                     AQVVQDFRQNGVTPQMLTRISNGSPLGQVFAAGLKNARSPTPIIKEAIEESGRTVAIE
                     LERFLTSLGTIAAIAPLLGLFGTVIGMIEIFGSQTPQGGNPLQMAHGISIALYNTAFG
                     LVVAVPAMIFYRHYRAKVDEMLVDMEMQAIKLVEILHGERQ"
     gene            3538..3957
                     /locus_tag="AC158Q_05705"
     CDS             3538..3957
                     /locus_tag="AC158Q_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR3339303.1"
                     /translation="MRFRPRAFREEPEINFIPLIDVLLVILIFLMVTTTYSRFAELQI
                     NLPTADAERQNERPDQIDVAVDAQGRYSINRSPAGFSNATVFSGELRRAAGDAKDPVV
                     VINADANAPHQSVINVMEAARIAGYGKITFTTQAGKK"
     gene            4051..5049
                     /gene="lpxK"
                     /locus_tag="AC158Q_05710"
     CDS             4051..5049
                     /gene="lpxK"
                     /locus_tag="AC158Q_05710"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - lipid-A 4'-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HIR3339304.1"
                     /translation="MAWIERHWQSVTPVSALLYPLSLLYGAVASIRRAASVPVRLPVP
                     VIVVGNITVGGTGKTPLVLWLAAFLKSRGYAPGIVSRGYGGSGRSPRRVLPESDPLDC
                     GDEAVLLARRGGCEVWAGADRVAAGEALIASQPACNVVLSDDGLQHYRLARDVELCVV
                     DAARGFGNGWLLPAGPLREPSSRLATVDAVVMRGRGVPHPSAGSVPAATARFSMTLEG
                     REFRGVAAPERRAAAEAFRGKRVHAVAGIGNPERYFDHLRALGLDFVAHPYPDHHPFA
                     AADLAFAGAEAVLMTEKDAVKCRAFANETHWSLAVDAAPDPGLGEFVLRKLGALRK"
     gene            5094..5273
                     /locus_tag="AC158Q_05715"
     CDS             5094..5273
                     /locus_tag="AC158Q_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HIR3339305.1"
                     /translation="MDSKLLDILVCPLCKGPLLYKKSAAELVCRPCRLAYPIKDDIPV
                     MLEDEARKLPPEEEV"
     gene            5346..>5503
                     /locus_tag="AC158Q_05720"
     CDS             5346..>5503
                     /locus_tag="AC158Q_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004567493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylyltransferase domain-containing protein"
                     /protein_id="HIR3339306.1"
                     /translation="MKFSVIIPARYASTRFPGKPLADVRGKPMVVRVAQRAGRSGADE
                     VLIATDHAA"
BASE COUNT          918 a         1892 c         1742 g          951 t
ORIGIN      
        1 gcatcgcggc cacccgccag ctcgccaagc gccagctcac ctggctgagg tcgatggaaa
       61 gcgcgaccga attcgactgc ctcgaggaaa gcctcgacga actggtgctg gagtacgtcc
      121 gccgcgagct cgaggcacaa ctcgttagtg actgacgagt tttgtcgaag gcaaaggcaa
      181 aaagagctgt aatccctttc gactttcgcc tttcgacttt cgccttaaaa gactccccat
      241 cacttgtgat gggaagtcat gtagcgcagg cgatcagcga gactcttgag cagcgccacg
      301 gcgaacgccg ggttggactt cacgagcccc aggaagtcgt tgcgcgtgat cgagagcagt
      361 tcgcagtccg tctcggccgt ggcggtggcg gcgcgcggcg actggtctat cagggccatc
      421 tcgccgaaga cgccgcctgc gcccacccgg ttgacgacct tggacttgat cgagatcgcc
      481 acctggccct cgagcacgat gtagcagaac accccgccct cgccttcctt catgatcacc
      541 ttgttcaggg gggcgtgcgc gggcggtcgg tgttgcaggt gttccacgag cgcggccagc
      601 aacggcgggt cgaacacccg gagctcctgg acctgcgcgg cgcccggcag gttgcggctc
      661 accagcgccg ccgtcaggcg caggcggttg atgatgatgt tcatcagcat gatcgcgaat
      721 tccggcgtcc tctggagcgc gctctggaac tgtcccgcat ccagcgacag cgtccggcag
      781 gcggtcttcg cggcggccgt ggcgctgcgc ggctggcggc tgatcgcggc catctcgccg
      841 aaaacctcgc cggccttgac cgtgtccagc accttcttcc cgcgtatcag gccgacctcg
      901 ccttccacca gcaggtacat cttgtcgctc gcctggttct cggtgaagaa cgtaccgccc
      961 tgcgacatga cctcgacgcg gcccagcgcc tcgaagcaca tcttcgcgat cgagggcttg
     1021 tagacctcgc tgctggcctg ccggttcgcg ttggtgaaat ccagatcatc cattggcttg
     1081 cctcatcttt ccggctccct gtcccctgcc gcgtccttgc gcacgacgcg cgcgccggcc
     1141 cgcccctttg caaatgttat tgtaacgtcc tcgccgatcg caagccgccc ggcgtcgcgc
     1201 acgatgaccc cgccggccgt ctcggtgatg ctgtagcctc gctccagcac ggattgcggg
     1261 ttgagatgct tgaggtttgc gtcgaggcgc gccagcagcg ccgcggcgcg ctcgagccgc
     1321 cgccgccccg tatcccgcag cctccgcgcc agtgcggcgg cgtcctgaat gcgccccccc
     1381 acgtcgggcg cgccggactt gagccgcagc cccagttcac gcacctgcca gcccaggtcc
     1441 tcccgcgccc gttcccaggc gccggcgagg cgcgtcgcaa ggtgccgcag ctcgctcacc
     1501 tggttgcgga tccgctcccc ggggtgaacc agccggcgcg acaggaagtc gagcttctgc
     1561 atgcggtctt cgagcccccg acggaacacg cgcagcagcc ggtgcgcctg ttgcagcagc
     1621 agccgggcca gctcgtcccg gccgggagtg gcaagcgcag ccgcgccggt cggcgtcggc
     1681 gccctcgcgt ccgcgacgaa atcggcaatg gtgaaatcgg tttcgtggcc gatgccggtg
     1741 acgaccggaa ccggcgaagc caggatggcg cgggcaacgg cctcttcgtt gaacgcccac
     1801 aggtcctcga tgctgccgcc gccccggcac acgacaagca cgtcgcactc ccgccgcgcg
     1861 ccagccagcg cgatcgccgc cgcaatacga tcccccgccc cttcgccctg caccggcgtg
     1921 ggataaagaa tcaccggcag gcccggcatg cgccgcttga gcgtcgtcag gacatcgcgc
     1981 aacgcggccg cctgcggcga ggtcaccact ccgaccgtac gcggaaagcg cggcagcggc
     2041 cgcttcctcg ccgggtcgaa caggccctcc ttctccagtt tcgccttcag gcgctcgaac
     2101 gcctcgtaga gcgcccccag gccggcgcgg cgcatcgctt cgacgttcag ctggaacttg
     2161 ccgcgcgctt cgtagagcgt gggcagcgcc cgcacctccg ccttcacccc gtcctgcggc
     2221 tgccagccga gcgcctgggc gcggccgcgg aacatcacgc agtccacctg ggcttgcgcg
     2281 tccttcaacg tgaagtagca gtggccggag tcgtagcgcc tgaagttgga gatctccccc
     2341 gccacccaga ggagcggcag gttgctctcc agcaattcgc gcgtgaggcg gttcagttcg
     2401 gcgaccgaga tgatttgcgg cagcggcaac cccggctggc gttcgagcat gccgcattct
     2461 aagtcagtgc tgaatgatga gtgataggtg atgagtgaaa tgaatgcggc gcaatgcgcg
     2521 gcctttggag cgaaattttc aattgaaatc aggagttgcg ggaaaatacg ggggcgccgc
     2581 gagggttttt ccttccacag ccgccatgga ttcgccgccg ctgccgcccc gaaacccgcg
     2641 tcccgggccg atctccgggc aagcgggtca acccgttgaa tccatgccct aaagtgttgc
     2701 ggtcaaattt taatcagcac agcaaaattc catatggaaa ctcgacttac atgaacgctc
     2761 gacagtgtat gcacatggtt atccacagcc attgtggaca accgcgcggc ttttttcgcg
     2821 cgccgcttcc aggaaaaaag caagcccgtt tttcgggccc ccatggttgc cgaaaacgca
     2881 ggcggcagat aaagttacgg ccactttcaa ggagtcactc acgtgttcgc catcatcgaa
     2941 gccgccggat ggcccatctg gtttctgatc gtcgcctccg tcatcgcgct ggccatcatc
     3001 ggggagcgct ggtggtcgct gcgcgagtcc gtcgtcatgc cgcgcaacct gctggcgcag
     3061 gtggtgcagg atttccgcca gaacggcgtg acgccgcaaa tgctcacgcg catctccaac
     3121 ggctccccgc tcgggcaggt gttcgccgcc gggctgaaaa acgccaggag cccgaccccc
     3181 atcatcaagg aggcgatcga ggagtccggg cgcacggtcg ccatcgagct cgagcgcttt
     3241 ctcaccagcc tcggcaccat cgccgccatc gcgccgctgc tcgggctgtt cggcaccgtg
     3301 atcggcatga tcgagatatt cggctcgcag accccgcagg gcggcaaccc cctgcagatg
     3361 gcgcacggca tctcgattgc gctctacaac acggcgttcg ggctggtggt cgcggtgccc
     3421 gccatgattt tctaccgtca ctaccgagcc aaggtggacg aaatgctggt ggacatggag
     3481 atgcaggcca tcaagctggt ggaaatcctc cacggcgaga ggcaatagga aagcaggatg
     3541 aggttccgcc cgcgcgcctt ccgcgaagag ccggagatca acttcatccc gttgatcgac
     3601 gtgctgctgg tgatcctgat cttcctcatg gtcaccacca cctactcgcg cttcgccgag
     3661 ttgcagatca acctgccgac cgcggatgcc gagcgccaga acgagcggcc cgaccagatc
     3721 gacgtcgcgg tggacgccca gggacgctac tcgatcaacc gcagcccggc ggggttcagc
     3781 aacgccaccg tcttcagcgg cgagctgcgc cgcgcggccg gcgacgccaa ggacccggtg
     3841 gtcgtgatca acgccgacgc caatgccccc caccagtccg tgatcaacgt catggaggcg
     3901 gcgcgcatcg cgggctacgg caagatcacc ttcaccacgc aagccgggaa gaagtgattc
     3961 gtttcccgtt cctcgtttct cgtttctcca tttccaccgg gttccgcctg acggcaacga
     4021 gcaacgagta acgagcaacg agtcactctg atggcctgga tagagcgcca ctggcaaagc
     4081 gtcactccgg tatccgccct gctctatccc ctgagcctgc tgtacggcgc ggtcgccagc
     4141 atccgccgtg ccgcatcggt ccccgtcagg ctgccggtcc cggtgatcgt ggtcggcaat
     4201 atcacggtgg gcgggaccgg caagacgccg ctggtgttgt ggctggcggc attcctgaag
     4261 tcccgcggtt acgcgccggg catcgtcagc cgcggctacg gcggcagcgg gcgctcgccg
     4321 cgccgggtgc tgcccgagtc cgatccgctc gactgcggcg acgaggccgt cctgcttgcg
     4381 cgccgcggcg ggtgcgaagt gtgggccggc gcggatcgcg tcgccgcggg cgaagcgctc
     4441 atcgcatcgc aaccagcctg caacgtcgtg ctgagcgatg acggcctgca gcattaccgg
     4501 ctcgcccgcg acgtcgagct ctgcgtggtg gatgccgcgc gcggcttcgg caacggctgg
     4561 ctgctgcccg cgggcccctt gcgcgaaccg tcgtcgcggc tggcaacggt tgacgccgtc
     4621 gtgatgcgcg gccgcggcgt gccgcatcca tccgccgggt cggtccccgc cgcaacggcc
     4681 cgcttctcga tgacgcttga agggcgcgaa tttcgaggcg tggccgcccc ggagcggcgc
     4741 gccgccgccg aggcatttcg cggaaaacgc gtgcacgccg tcgccgggat cggcaacccg
     4801 gaacgctact tcgaccatct ccgggcgctg ggtctcgact tcgtcgccca tccttacccc
     4861 gaccatcatc cttttgccgc cgccgacctc gcatttgccg gggcggaagc ggtcctgatg
     4921 acggagaagg atgcggtaaa atgccgggca ttcgcgaacg agacgcactg gtcgctcgcg
     4981 gtcgacgcgg cgccggaccc ggggctgggc gagttcgtgc tgcgcaaact gggggcttta
     5041 cgaaaatgat ccttagccgt cactcgtcat tcgtcacccg ccacgggtcg taaatggaca
     5101 gcaaactgct cgacatactg gtgtgcccgc tgtgcaaggg cccgctgctg tacaagaaat
     5161 ccgcggccga gctcgtctgc aggccctgcc gcctcgccta tcccatcaag gacgacatcc
     5221 cggtgatgct cgaggacgaa gcgcgcaaac ttcctcccga ggaggaagtt tgaaagaagt
     5281 gactcgtttc tcgttgctcg tttctggtgt tggcgaggac gagtaacgag gaacgaatca
     5341 ctcctttgaa gttctccgta atcattcccg cccgctacgc ctctacgcgc ttccccggca
     5401 agccgctcgc ggacgtgcgc ggcaagccga tggtggtgcg cgtggcgcag cgggcgggca
     5461 gaagcggggc cgacgaggtg ctgatcgcca ccgaccacgc ggc
//
LOCUS       DBNGSG010000214         3013 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2105562, whole genome shotgun sequence.
ACCESSION   DBNGSG010000214 DBNGSG010000000
VERSION     DBNGSG010000214.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2105562"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..937)
                     /locus_tag="AC158Q_05725"
     CDS             complement(188..937)
                     /locus_tag="AC158Q_05725"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3339307.1"
                     /translation="MKRWGSGEPLVLLHGGMGSWRHWIRNIDALAGTFAVHALDLPGC
                     GDSDSVPRDISDDDYITRVEEAIAGIGGRQPVRLAGFSFGGILSAMVAARMGTQIRKL
                     SLLSPGGFGKSPRTLDLRKIPPDTAGLAAVREVLRHNLGVMMLADPACITEDTVDLHY
                     ANVRRTRFDGRRFSVSNRMMETLGHIVCPVQTIWGERDALPYPDVRARIAVCRKAVSN
                     IRVDLVPGAGHWVQYEAPEPVNRALLDFLVN"
     gene            complement(1088..2755)
                     /gene="ettA"
                     /locus_tag="AC158Q_05730"
     CDS             complement(1088..2755)
                     /gene="ettA"
                     /locus_tag="AC158Q_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HIR3339308.1"
                     /translation="MAQYVYTMRRVGKVVPPKRVILKDISLSFFPGAKIGVLGLNGAG
                     KSSLLKIMAGVDTAFEGEAVPMPNMRVGFLPQEPQLDANKTVRETVEEGMGEVMEAKK
                     KLEAIYAAYSEEGADFDKLAAEQSKYEAIVAASGGGETDHQLEIAADALRLPPWEAKI
                     GPLSGGEKRRVALCRLLLSKPDMLLLDEPTNHLDAESVDWLEQYLQKFPGTVVAVTHD
                     RYFLDNAAQWILELDRGYGIPWEGNYSSWLDQKQTRLKQEEATESARQKALKKELEWV
                     RQNPKGRQAKAKARIARFEELNSYEYQKRNETQEIFIPVAERLGDSVIEFKGVSKGYG
                     DRLLIDDLSFSVPPGAIVGIIGPNGAGKSTLFRMITGAEKPDRGKVAIGSTVRLAHVD
                     QSRDKLANEQTVFEFVSGGTDILKVGKFEMPTRAYLGRFNFKGTDQQKKVGNLSGGER
                     GRLHLAKATLSGGNVLLLDEPSNDLDVETLRALEDALLEFAGTVLVISHDRWFLDRIA
                     PHILAFEGDSKVALFNGNYQEYEADKKKRLGEEGAQPHRIRFKPLKA"
     gene            2838..>3013
                     /locus_tag="AC158Q_05735"
     CDS             2838..>3013
                     /locus_tag="AC158Q_05735"
                     /inference="COORDINATES: protein motif:HMM:NF022018.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HIR3339309.1"
                     /translation="MAVRSKAIVATTRIEQSILLLRGQKVLLGSTLAELYGVQPKVLM
                     QAVQRNRLRFPADF"
BASE COUNT          489 a         1011 c          944 g          569 t
ORIGIN      
        1 cgtaagcgga agcgtcccgc ctatcatatc cgacggcggc cagactagaa atcatctcaa
       61 aaaccgtttt agccacggaa acacacggaa ccacacagaa attcctgaat gaatacgggg
      121 actccaaggg gtcggcctat tgtccgtgtc attctgtgtg attccgtggc tattattgag
      181 actggttcta gttaaccagg aaatcgagca gggcgcggtt gaccggttcc ggcgcctcgt
      241 actgcaccca gtggccggcg ccgggaacga ggtccacgcg gatgttggag acggccttgc
      301 ggcagacggc tatgcgcgcg cgcacgtccg ggtacggcag cgcgtcgcgc tccccccaga
      361 tcgtctggac cgggcacacg atgtgcccga gcgtttccat catgcggttg ctcacgctga
      421 agcggcggcc gtcgaagcgc gtgcgccgca cgttggcgta gtgcaggtcc accgtgtcct
      481 ccgtgatgca cgccgggtcg gcgagcatca tgacgcccag gttgtggcgc agcacctcgc
      541 gcacggccgc caggcccgcg gtatcgggcg gaatcttgcg caggtcgaga gtcctcggcg
      601 atttgccgaa gccgcccggg ctcagcagcg atagcttgcg tatctgcgtt cccatgcgcg
      661 cggccaccat cgccgacagg atgccgccga atgagaagcc ggcgaggcgg accggctgcc
      721 tgccgccgat cccggcgatc gcctcttcca cccgggtgat gtaatcgtcg tccgaaatgt
      781 cgcgcggcac cgagtcggag tcgccgcaac ccggtaggtc cagcgcgtgc acggcgaatg
      841 tcccggcgag cgcgtcgata ttgcgtatcc agtgccgcca cgagcccatg cccccgtgca
      901 gcagcaccag cggctcgcca ctgccccacc gcttcacagc caagccgcca atgcggcttg
      961 tctgacactc atcacccata attcatcgcc ctaccccctc cctcaccctc ccccttggca
     1021 agggggaggg ataggggggg ggttgtcacc cgtcactcga cactcgacac tcgacaccgc
     1081 atcaggttca agccttgagt ggcttgaacc tgatgcgatg cggctgcgcc ccctcttccc
     1141 ccaggcgctt cttcttgtcc gcctcgtatt cctggtaatt gccgttgaat agcgccacct
     1201 tcgaatcgcc ctcgaacgcg aggatgtggg gggcgatgcg gtcgaggaac cagcggtcgt
     1261 gggatatgac gagcaccgtg ccggcgaact ccagtagcgc gtcttccagg gcgcgcagcg
     1321 tctccacgtc gaggtcgttg gacggctcgt cgagcagcag cacgttgccg ccggagagcg
     1381 tggccttggc gagatgcagc cgcccgcgct cgccgcccga gaggttgccc actttcttct
     1441 gctggtcggt gcccttgaaa ttgaagcggc cgaggtaggc gcgcgtcggc atctcgaact
     1501 tgccgacctt caggatgtcg gtgccgccgg agacgaactc gaacacggtc tgctcgttcg
     1561 cgagcttgtc gcgcgactgg tccacgtgcg cgagcctgac cgtcgagccg atcgccacct
     1621 tgccgcggtc cggcttctcc gccccggtga tcatgcggaa cagcgtggac ttgccggcgc
     1681 cgttcgggcc gatgatgccg acgatcgcgc ccggcggcac gctgaaggaa agatcgtcga
     1741 tcagcaggcg gtccccgtag cccttggaca cgcccttgaa ctcgatcacg gaatcgccca
     1801 ggcgctcggc gacgggaatg aagatttcct gggtctcgtt ccgcttctgg tactcgtacg
     1861 agttaagctc ctcgaagcgg gcgatgcgcg ccttggcctt ggcctgccgc cccttggggt
     1921 tctggcgcac ccattccagc tctttcttca gcgctttctg gcgcgccgac tcggtcgctt
     1981 cttcctgctt cagccgcgtc tgcttctgat cgagccacga gctgtagttg ccctcccacg
     2041 ggatgccgta gccgcggtcg agctcgagga tccactgcgc ggcgttgtcg aggaagtagc
     2101 ggtcgtgggt caccgccacc acggtgccgg ggaatttctg caggtactgc tccagccagt
     2161 ccacgctctc ggcgtcgaga tggttggtcg gctcgtccag cagcagcatg tcgggcttcg
     2221 acagcagcag ccggcacagc gccacccggc gcttctcgcc gccggacaac ggcccgatct
     2281 tcgcctccca cggcggcagg cgcagcgcgt cggcggcgat ttcgagctgg tggtcggtct
     2341 cgccgccgcc gctcgccgcg acgatcgcct cgtatttgct ctgctcggcg gcgagcttgt
     2401 cgaaatccgc gccttcctcg gaataggccg cgtagatggc ttccagcttc ttcttcgcct
     2461 ccatcacctc gcccatgcct tcttccaccg tctcgcgcac ggtcttgttt gcgtcaagct
     2521 gcggctcctg cgggaggaag ccgacgcgca tgttcggcat cgggaccgcc tcgccctcga
     2581 acgcggtatc cacccccgcc atgattttca gcaggctcga cttgccggcg ccgttcaggc
     2641 ccagcacgcc gatcttggcg ccgggaaaaa aggagagcga aatgtccttc aggataacgc
     2701 gcttgggcgg cacgaccttg ccgacccggc gcatggtgta gacgtattgg gccatgggta
     2761 tttgcgctga aagggcgcaa tcataccgtg gtcaacgcac gcccggccat gttcgttatc
     2821 tggctggagg agagaaaatg gctgtgcgca gcaaggcaat tgtcgccacc acgcggatcg
     2881 agcaatcgat cctgctgctg cgcggccaga aggtgctgct aggctccacc ctcgcggagc
     2941 tgtacggcgt gcaaccgaaa gtcctcatgc aggcggtgca gcgaaaccgc ttgcggttcc
     3001 ccgcggattt cat
//
LOCUS       DBNGSG010000215         2780 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1766498, whole genome shotgun sequence.
ACCESSION   DBNGSG010000215 DBNGSG010000000
VERSION     DBNGSG010000215.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1766498"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..960
                     /locus_tag="AC158Q_05740"
                     /pseudo
     CDS             1..960
                     /locus_tag="AC158Q_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011408623.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1047..1721)
                     /gene="rnc"
                     /locus_tag="AC158Q_05745"
     CDS             complement(1047..1721)
                     /gene="rnc"
                     /locus_tag="AC158Q_05745"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015065099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HIR3339310.1"
                     /translation="MSEADLPARLGYRFRDPGLLRQALTHRSYGAPHNERLEFLGDGV
                     LNCVIAWLLFRRFPGLPEGNLSRLRANLVNQDSLSRLALSLDLGEYLLLGEGELKTGG
                     FRRPSILADALEAVLGAVFLDGRFEAAAEVVERLYDPLMAELDPRTQGKDPKTLLQEL
                     LQARRLPLPRYNVVSVHGEAHEQHFHVECLIPELGIRTEGEGPSRRSAEQSAARAAYE
                     TAVDSE"
     gene            complement(1738..2094)
                     /locus_tag="AC158Q_05750"
     CDS             complement(1738..2094)
                     /locus_tag="AC158Q_05750"
                     /inference="COORDINATES: protein motif:HMM:NF027463.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HIR3339311.1"
                     /translation="MRTQRGLSLIGLLLVSGLLVLLALIGFKVLPAYVEYLAVKKAVT
                     DIARAPETRGGQVRDVQMAFDRRAQIDDITSIRGQDLEINKLGDGFEIIATYSVQVPL
                     FGNVSACIDFVAEGGR"
     gene            complement(2123..>2780)
                     /gene="lepB"
                     /locus_tag="AC158Q_05755"
     CDS             complement(2123..>2780)
                     /gene="lepB"
                     /locus_tag="AC158Q_05755"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR3339312.1"
                     /translation="LLVFLLRSFLVEPFKIPSGSMIPTLLVGDFILVNKYTYGIRLPV
                     INVKVLDINRPQRGEVMVFRYPDNPALDYIKRVVGEPGDTVAYRNKQLFVNGEAVPMT
                     PESDYQYAEGGLNYVTTKQLRETLGEHNHAVLINPDVPPMQVAGVRDFPHRENCEYNE
                     SGFQCKVPPGHYFLMGDNRDSSSDSRYWGFVPERNIVGKAFMIWWNFSDFGRIGTRIN
                     "
BASE COUNT          461 a          924 c          908 g          487 t
ORIGIN      
        1 gtgggggggc aagctctctg gcttcaccct gttgttcgag gcgctggtgc tcatgctgtg
       61 ccagcagatg accttcgccg cggccgcgcg cctggtcggc gagagctggc accgggtggc
      121 cgccatctgc gagcgctatg tggagctggc gctggcgcag gcggacttct cgggggtgcg
      181 cgagctggcc atcgacgaga cctcgcgcgc acgtggccat gactacatca cgctggccgc
      241 cgacgcgctg gagcgtcgcg tactggcggt ggccgaaggt cgcagcctgt ggagaccatc
      301 gaagcgctgg caggcgaact ggaggcccat ggctgcgtcc cagcgcaagt cagctcggtg
      361 agcatcgaca tgtccccggc gttcatcaag ggttgcggtg agcgcctgcc caacgcccgt
      421 atcaccttcg acaagtccca cgtcgtctgg catgccagca ctgcggtgga caagatgcgc
      481 cgtatcgggc agcgcaccga ccgctcgctg aagggcatgc gctggtcgct gctcaaggat
      541 cgctcccgcc tctcgcccga ggccgcagcc gacctggacg ccttgatcgc cagaatgacc
      601 acggtgcgca ccgcccgcgc ctgggtctac aaggaacagc tgcgcgagat cctcgagcgc
      661 aagcagatca acgtcgtgcg cgccatgctc cagcactggt gtagctgcgt gatgcgctcc
      721 aaggtcgagc cgatgaagga ggtcgccgag ctcgtgcgcc gccacctgga aggcatcgtg
      781 gcctgggcgc agacgcgaca gaccaacggc ttcctggaag ctctcaatgg cctgttccaa
      841 gccgccaagc gccgcgcccg cggcttcact cgcatgtcca cgatccggac ggtcatcttc
      901 ctgattgccg gcaagctcga cttccgcgcg atcaacccgc atgccggtca acccacttga
      961 aattcaacag agccttttta tctgcgcggc ggggcggtgt ggtgtgcggc gtcatcgctc
     1021 gcgccaaaac gtggatcgga ggtttctcac tccgaatcca cggcggtttc gtacgcggcg
     1081 cgtgcggcgc tctgctcggc gctgcgccgg ctcggtccct cgccctcggt gcggatgccg
     1141 agctcgggga tcaggcattc cacgtggaag tgctgctcat gcgcctcgcc gtgtacgctc
     1201 accacgttgt agcgcggcag cggcaggcgc cgcgcctgca gcagttcctg cagcagggtc
     1261 ttcgggtcct tgccctgagt tcggggatcg agctccgcca tcaggggatc gtagagacgc
     1321 tccaccacct ccgcggccgc ttcgaaccgg ccgtccagga acaccgcgcc gagcaccgct
     1381 tccagggcgt cggcgaggat agacggacgc cggaaaccgc cggtcttgag ctcgccctcg
     1441 cccagcagca ggtactcgcc gaggtccagg ctcagggcca ggcgagacaa gctgtcctgg
     1501 ttcaccaggt tggcgcgcaa ccgcgacagg ttgccttcgg gcaatcctgg gaagcgccga
     1561 aacagcagcc aggcgatcac gcagttgagc acgccgtcgc cgaggaattc caggcgctcg
     1621 ttgtgcgggg cgccgtagct gcgatgggtg agcgcctgcc gcagcaggcc cgggtcccgg
     1681 aagcggtagc ccagacgggc aggcagatcc gcttcgctca tgctccccga cggcgcgtca
     1741 tctcccgccc tcggcgacga aatcgatgca ggcgctgacg ttgccgaaca gagggacctg
     1801 caccgagtac gtggcgatga tctcgaagcc gtcccccagc ttgttgatct cgaggtcctg
     1861 gccgcggatc gacgtgatgt cgtcgatctg ggcgcggcgg tcgaacgcca tctgcacgtc
     1921 gcgcacctga ccgccgcgcg tctccggagc gcgggcgatg tcggtcacgg ctttcttcac
     1981 cgccagatac tcgacgtagg ccggcagcac cttgaagccg atgagggcaa gcaataccag
     2041 cagcccggaa accagcagca gtccgatcag gctcaatccc ctttgcgttc gcatagcgtt
     2101 ctccgccttg tgccgggttg tgctaattga tgcgcgtgcc gatgcggccg aagtcgctga
     2161 agttccacca gatcatgaag gccttgccga cgatgttgcg ctccggcacg aagccccagt
     2221 aacggctgtc gctgctgctg tcgcggttgt cgcccatcag gaagtagtgc cccggcggca
     2281 ccttgcactg gaatcccgat tcattgtatt cgcagttctc gcggtgcgga aaatcgcgca
     2341 cgcccgcgac ttgcatcggc ggcacgtccg ggttgatgag cacggcatgg ttgtgctccc
     2401 cgagcgtctc gcgcagctgc ttcgtcgtca cgtagttgag gccgccttcg gcgtactggt
     2461 aatccgattc gggtgtcatc ggcaccgcct cgccgttcac gaacagctgc ttgttgcggt
     2521 aggccacggt gtcgcccggc tcgcccacca cgcgcttgat gtagtccagg gccgggttgt
     2581 cgggatagcg gaacaccatc acctcgccgc gctgcggccg gttgatgtcc agcaccttga
     2641 cgttgatcac cggcagccgg atgccatagg tgtacttgtt gaccaggatg aaatcgccca
     2701 ccagcagagt cgggatcatc gagcccgacg gaatcttgaa cggctccacc aggaaggagc
     2761 gcagcaggaa caccagcagg
//
LOCUS       DBNGSG010000216         1593 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_408271, whole genome shotgun sequence.
ACCESSION   DBNGSG010000216 DBNGSG010000000
VERSION     DBNGSG010000216.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_408271"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..867)
                     /locus_tag="AC158Q_05760"
     CDS             complement(136..867)
                     /locus_tag="AC158Q_05760"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004528684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042178 - xenobiotic catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HIR3339313.1"
                     /translation="MKYYLYYWPHIQGRGEFVRLALEEAGADYVDVGRLPESEGGGRP
                     GVARMLERAADGRTPFAPPVLKAGALLISQTANILQYLGPRLGLVPKAEAARLWAHQL
                     QLVLADFVVEAHDTHHPVAKSLYYEEQKPESLRRSENFRALRLPKFLGYFERLLETNP
                     GGDRHLVGNRLTYVDLSMFQVIAGLEYAFPKTMQGMSPKYPRLSGLRRMVMQRPRIAA
                     YLASTRRLPFNQHGLFRHYPELDQG"
     gene            complement(1028..1477)
                     /locus_tag="AC158Q_05765"
     CDS             complement(1028..1477)
                     /locus_tag="AC158Q_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3339314.1"
                     /translation="MSAVTPTAANHANLPPLASRFVSVGALPWEKTRHAGVETKALVS
                     DPASGLLTVLTKMAPGAKLPDHEHVLIEQTYVLEGAIHCGEGVCRAGDFVWRPAGSRH
                     EAWAGPEGCVTLAMFQMPNRFFEPGGKVVDFLGNDWDKTWSSLRGPR"
BASE COUNT          317 a          499 c          493 g          284 t
ORIGIN      
        1 agaaatacac tcaaactcaa aaactgatgg ctgaaacaaa cgagctgatc cgtatctttg
       61 tcagtgtagt acggttagta aaaaccccag ctccggtcta gggattcttt ttcctcatcc
      121 ctcatccctc tgccctcatc cctgatccaa ttcgggataa tgacgaaaaa gtccgtgctg
      181 gttgaacggc aggcgccggg tggaagccag gtaggcggcg atgcgcgggc gttgcatgac
      241 catccggcgg agccccgaca ggcgcgggta tttcggtgac atgccctgca tcgttttcgg
      301 gaaggcgtat tcgagccccg cgatcacctg gaacatcgag aggtccacgt aggtcagcct
      361 gttcccgacc aggtggcggt cgccgccggg attggtttcg agcagtcgct cgaagtagcc
      421 gaggaacttc ggcagccgca gcgccctgaa attctccgac cgccgcagcg actccggctt
      481 ctgctcctcg taatagaggc ttttcgcgac cgggtggtgc gtgtcgtgcg cctcgaccac
      541 gaaatcggcg agcacgagct gcagctggtg cgcccacagc cgcgccgcct ccgctttcgg
      601 cacgagaccg agccgcggcc ccaggtactg taggatgttc gcggtctgcg agatcagcag
      661 cgcgcccgcc ttcaataccg gcggcgcgaa cggcgtgcgc ccgtccgccg ctctctccag
      721 catgcgggcg acccccggcc ggcccccgcc ctcgctctcc ggcaggcgcc cgacatccac
      781 gtaatccgcc ccggcctcct ccagggcgag ccggacgaat tcaccgcggc cttgtatatg
      841 aggccagtag tagaggtagt acttcataag ggatgagggc ggaggtatga ggaaaataat
      901 gtcgaggatt gaatgtcgag tgaacgtgag ggggaggagt gtcgaggaat gagtgtcggg
      961 tgaaaaaacg taatggtagc tttcttccct cgaccctcga cacccgacac tcgacacccg
     1021 tcactcgtca ccgcggcccg cgcagggagc tccaggtttt atcccagtcg ttgccgagaa
     1081 aatcgacgac cttgccgccg ggctcgaaga agcggttggg catctggaac atcgcgagcg
     1141 tgacgcagcc ctcgggaccg gcccaggctt cgtggcggct gccggcgggg cgccagacga
     1201 agtcgccggc gcggcacacg ccctcgccgc aatggatggc gccttcgagc acgtaggtct
     1261 gctcgatcag cacgtgctcg tggtcgggca gcttcgcgcc cggtgccatc ttcgtcagca
     1321 cggtcagcag gccggacgcg ggatcggaca ccagcgcctt ggtctcgacg ccggcgtgcc
     1381 gggttttttc ccagggcaat gccccgacgc tcacgaagcg cgaagcgagc ggcggcaggt
     1441 tggcgtggtt cgctgctgtc ggcgtgactg cgctcatctg caacctcctc ccgtcccgga
     1501 aaatcacgaa cagacaggat cagcaagact tgaacaagaa aaccgaaaac ccgtcaactt
     1561 cgtaaatgga caggattaac aagattaaca aga
//
LOCUS       DBNGSG010000217         2742 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1767313, whole genome shotgun sequence.
ACCESSION   DBNGSG010000217 DBNGSG010000000
VERSION     DBNGSG010000217.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1767313"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1162
                     /locus_tag="AC158Q_05770"
     CDS             <1..1162
                     /locus_tag="AC158Q_05770"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SufS family cysteine desulfurase"
                     /protein_id="HIR3339315.1"
                     /translation="GKPLVFLDNAASSQMPQPVIDRWVRYQTTQHSNIHRAVHHLSEV
                     ATAEYEGARRKLQAFINAKEDREVIFTTGTTDAINLVAHGYGRKFIGRDDEIVLTTLE
                     HHSNIVPWQMVAEEKGAKIRVVPVNDRGELIHEEYLKLLGPRTRIVGVGHVSNALGTV
                     NPVREMIEAAHAKGIPVLVDGAQAAPHMKLDMQALDCDFYAFSGHKMFGPTGIGILYG
                     KAAWLEKMQPFKGGGDMILSVTFEKTTYNVPPHKFEAGTPPIAAAITLGAAVDYIDSI
                     GLSRIHAHETDLLDYATDQVNRLPGVRIIGTAEKKAAVLSFVVDGVHPHDVGTLLNEE
                     GVAIRTGHHCAQPVMQRFGIPATSRASFAFYNTHAEVDALVAGIKRVQKVFK"
     gene            1214..1696
                     /gene="sufU"
                     /locus_tag="AC158Q_05775"
     CDS             1214..1696
                     /gene="sufU"
                     /locus_tag="AC158Q_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HIR3339316.1"
                     /translation="MDSKQLYQEVILDHNRKPRNWGVLADATHKAEGHNPLCGDHLTL
                     TLKLAGDAVDAIGFQGESCAICKASASMMTTAVKGKRTADAEQMIREFRDMATGQLDA
                     AAPNHLGRLTVFAGVRDLPTRVKCAILPWHTLHAALNSEASVTTEADADPMNAPVGNA
                     "
     gene            1908..2474
                     /locus_tag="AC158Q_05780"
     CDS             1908..2474
                     /locus_tag="AC158Q_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HIR3339317.1"
                     /translation="MPKIAEIEPTPNPNAMKFILKEPLTWGISRSYENAGEAQGDRLA
                     EALFDIEHVSSVFYVDHWITVTQDGQADWQELMRKLAVPIREAPAADADSAKYAEKAI
                     AITETAELSDADKARLEKINEILDEQIRPYLQGDGGDLYVVGLEGNKLMVHYQGACGS
                     CPSSISGTLTGIENLVRQIEPEMEVVAV"
BASE COUNT          615 a          825 c          864 g          438 t
ORIGIN      
        1 cggcaagccg ctggtgttcc tggacaacgc ggcttcgagc cagatgccgc agccggtgat
       61 cgaccgctgg gtccgctacc agaccaccca gcattccaac atccaccgcg cggtgcacca
      121 cctgtccgaa gtcgccacgg cggaatacga gggcgcgcgc aggaaactgc aggcgttcat
      181 caacgccaaa gaggaccgcg aggtcatctt caccaccggc accaccgacg ccatcaacct
      241 ggtcgcgcac ggttacggcc gcaagttcat cgggcgcgac gacgagatcg tgctgaccac
      301 gctcgagcat cactcgaaca tcgtgccgtg gcagatggtc gcggaagaga aaggcgcgaa
      361 gatccgcgtc gtgccggtca acgaccgcgg cgagctcatt cacgaggaat acctgaagct
      421 gctcggcccg cgcacgagga tcgtgggcgt cggccacgtc tccaacgcgc tcggcaccgt
      481 caacccggta cgcgaaatga tcgaggccgc gcacgcaaag ggcatcccgg tgctggtgga
      541 cggcgcgcag gcggctccgc acatgaagct cgacatgcag gcgctcgact gcgatttcta
      601 tgctttctca gggcacaaga tgttcgggcc gaccggcatc ggcatcctgt acggcaaggc
      661 cgcatggctg gagaagatgc aacccttcaa gggcggcggt gacatgattc tctcggtcac
      721 cttcgagaag actacctaca acgtgccgcc ccacaagttc gaggccggca cgccgccgat
      781 cgccgcggcg atcacgctcg gcgcggcggt ggattatatt gattcgatcg gcctctctcg
      841 catccacgcg cacgaaacgg atttactcga ttacgcgacc gatcaggtga accgcctgcc
      901 gggcgtgcgc atcatcggca ccgcggagaa gaaggcggca gtgctgtcct tcgtcgtgga
      961 cggcgtgcat ccgcacgacg tcggcacgct gctgaacgag gagggggtgg cgatccgcac
     1021 cggccaccac tgcgcccagc cggtgatgca gcgcttcggc atccccgcta cctcgcgcgc
     1081 gtcgttcgcc ttctacaaca cccacgccga agtggacgca ctggtcgccg gcatcaagcg
     1141 ggtgcagaag gttttcaagt gattttttgc atacgagcaa cgagaaacga gctacgagtc
     1201 acttcttgag gttatggaca gcaaacagct ctaccaggaa gtgatcctcg atcacaaccg
     1261 caagccgcgc aactggggcg tgctggcgga cgcgacgcac aaggcggaag ggcacaaccc
     1321 gctgtgcggc gaccacctga cgcttacgct gaagctggcc ggcgacgcgg tggatgccat
     1381 cggcttccag ggcgagtcgt gcgctatctg caaggcgtcg gcctcgatga tgacgaccgc
     1441 ggtcaagggc aaacgcaccg ccgatgccga gcagatgatc cgggagttcc gcgacatggc
     1501 gaccggccag ctcgatgcag cggccccgaa ccatctcggc cgcctcacgg tatttgccgg
     1561 ggtgcgcgac ctgccgacgc gggtgaagtg cgcgattctg ccgtggcaca cgctgcacgc
     1621 ggcgctcaac tcggaagcga gcgtcaccac cgaagccgac gccgacccga tgaacgcccc
     1681 ggtcggtaat gcgtaataaa ttagccgcca agacgccaag gccgccaagg agagcaagga
     1741 taataaggca ggttttccag acgtaacggg acaaacgggg cttggataga gaaggacaaa
     1801 gtagggctgg ttcgagacag gtagtcatga tgaagatgaa tggttttatt ttgatattgg
     1861 tctccttggc gttcttggcg tcttggcggc taatttcggg ttgaacaatg ccgaagatcg
     1921 ctgaaatcga gccgacgccg aacccgaacg cgatgaagtt catcctcaag gagccgttga
     1981 cctggggaat atcgcgctcg tacgagaacg cgggcgaagc gcagggcgac aggctcgccg
     2041 aggcgctgtt cgacatcgag cacgtgtcga gcgtgttcta cgtggaccac tggatcacgg
     2101 tcacgcagga cggccaggcc gactggcagg agttgatgcg caagctcgcg gtgccgatcc
     2161 gcgaggcgcc ggccgccgac gcggactcgg cgaaatacgc ggagaaagcc atcgcgataa
     2221 ccgagaccgc ggagctgtcg gacgcggaca aggcgcgact ggaaaagatc aacgagatcc
     2281 tcgacgagca gatacgcccg tacctgcagg gcgacggcgg cgacctctat gtcgtggggc
     2341 tggaaggcaa caagctcatg gtgcactacc agggcgcgtg cgggagttgc ccgagctcga
     2401 tctccggcac gcttaccggg atcgagaacc tcgtgcggca gatcgagccg gagatggaag
     2461 tcgtggccgt gtagaaatta gccgccaaga cgccaaggcg ccaagaagaa ctaaaataaa
     2521 taattgaacc gccaaggccg ccaagaagaa gaatgacaaa tagtgtgaac taacaatcaa
     2581 ctaattcacg ttaaaagcaa agtgaggccg ggtccacttt tctggaaatg tgaacccagc
     2641 cccatttgac cttcacctgc tcgcgcggac gatcggaaca cgttagacag atacttatga
     2701 ggatgcaatc gtgacagcgg gagaatctga ctacctgctt cg
//
LOCUS       DBNGSG010000218         2284 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1937051, whole genome shotgun sequence.
ACCESSION   DBNGSG010000218 DBNGSG010000000
VERSION     DBNGSG010000218.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1937051"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="AC158Q_05785"
     CDS             <1..549
                     /locus_tag="AC158Q_05785"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3339318.1"
                     /translation="WISLAAVLFSAVQRILLSFTVIYLVAEGGYGLVEAGVMLSLAQI
                     GGSIARIPWGWLADRLKSGLAVLTIICVIMIITSIALVALDATWPKPLVYLLFLLLGA
                     SCVGWNGIVHAECARLSPPGSISLVAGGTSFFVYGGVMMGPPVFAVAYGAIGSYGTTF
                     WLMVAGAVAALGLLWLARRHKG"
     gene            575..1321
                     /locus_tag="AC158Q_05790"
     CDS             575..1321
                     /locus_tag="AC158Q_05790"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HIR3339319.1"
                     /translation="MDALGAFELVFAALVLVVAYAVRGTAGFGGQAVAVPLLALVLPL
                     PVMVSAVVVLTALASLGHWLRDWNRIEWREIARLLPWTAIGVLTGLYLLELVDVHVLT
                     RAFGAFVILYACFALATATRPVRVPARYRGPGRAILSTLAGIAGAAFGAAAGPLYAMH
                     FDSMRMEKNAFRVTITTILGIQALMRVAGYARLGFYDGTVVILIVAGLPLMVLGTRIG
                     HWLAGRLDPHRFSLGISVLLLMSGTALLFK"
     gene            1327..2073
                     /locus_tag="AC158Q_05795"
     CDS             1327..2073
                     /locus_tag="AC158Q_05795"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HIR3339320.1"
                     /translation="MPEGFSVFQIAVSACIVFGAFVVRGMSGFGAGMIGIPLLAFLMP
                     VHTAVAMFGLLVLSLFVFLSVRDWGEVVKDELKRLIAPTLLGVAAGVLLFARLDNHLL
                     LQLLGGFLIGYALYSFAVHTFGLPEFTCSPRWAAPIGFAGAFIDTLFGGGGGTLVVIY
                     LHMRKVGRAGFRATVAALWLAEMIARIAGYGAAGYYSADILLLCALLLPLMAAGTWVG
                     ERLGNRISQEAFSRLLALLLALAGVSLLLK"
     gene            complement(2084..2221)
                     /locus_tag="AC158Q_05800"
     CDS             complement(2084..2221)
                     /locus_tag="AC158Q_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339321.1"
                     /translation="MAFYAFYVVAIVILVLHFTGWLKRNNLEWLVLVLAGATFPAVLF
                     L"
BASE COUNT          301 a          709 c          803 g          471 t
ORIGIN      
        1 tggatttcac tcgccgcggt gctgttctcc gccgtccagc gcatcctgct ctccttcacc
       61 gtgatttatc tcgtcgcgga aggcggctac ggcctggtgg aggcgggggt catgctgtct
      121 ctcgcgcaga tcggcggctc gatcgcccgg attccctggg gctggctggc cgaccgcctg
      181 aaaagcggtc tcgccgtgct caccatcata tgcgtaatca tgatcatcac ctcgatcgcg
      241 ctggtcgcgc tcgacgcaac ctggccgaag ccgctggtgt atctgctgtt cctgctgctg
      301 ggcgcgagct gcgtgggctg gaacggcatc gtgcacgcgg agtgcgcgag attgagtccg
      361 cccggctcga tcagcctggt cgcgggagga acctcgtttt tcgtgtacgg cggagtgatg
      421 atggggccgc cggtgttcgc ggtggcctac ggggcgatcg gatcttacgg cacgactttc
      481 tggctgatgg ttgccggcgc cgtcgcggcg ctgggactcc tgtggcttgc gcgccggcac
      541 aagggatgag cgccggccgc gctccggtag ccagatggat gcgctaggcg ccttcgagct
      601 ggtattcgcg gcgttggtcc tggtcgtggc ctatgccgtg cgcggtactg ccggctttgg
      661 cgggcaggcg gtcgcggtgc cgctgcttgc cctggtgctg ccgttgccgg tgatggtgtc
      721 ggccgtggtc gtgctgaccg cgctggcgtc gctgggtcac tggctgcggg actggaaccg
      781 gatcgaatgg cgcgagatcg cccggctgct gccctggacc gcgataggcg tgctcaccgg
      841 gctgtacctg ctcgagctgg tggacgtcca cgtgctgaca cgggcgttcg gcgcgttcgt
      901 gatcctgtat gcgtgctttg cgcttgcgac ggcgacccgg ccggtgcgcg tgccggcgcg
      961 ctaccggggg ccgggacgcg cgatactgtc cacgctggcg ggcatcgcgg gcgcggcctt
     1021 cggcgccgcg gcgggtccgc tctacgccat gcatttcgat tcgatgcgga tggaaaagaa
     1081 tgcgttccgc gtgaccatta ccacgatact ggggatacag gcgctgatgc gcgtcgcggg
     1141 ttacgcgcgg ctcggcttct atgacgggac cgtcgtgatc ctcatcgtcg cgggactgcc
     1201 gctgatggtc ctgggcacgc gcatcggcca ctggctggcc ggtcggctcg acccgcatcg
     1261 attcagcctc ggcatcagcg tgctgctgct gatgagcggc acggcgctcc tgttcaagta
     1321 ggcgccatgc cggaagggtt cagcgtattt cagatcgccg tctcggcctg catcgtgttc
     1381 ggcgccttcg tcgtgcgcgg catgtcggga ttcggcgcgg gcatgatcgg catcccgctg
     1441 ctcgcattcc tgatgccggt gcacacggcg gtggccatgt tcgggctgct ggtgctgagt
     1501 ctcttcgtgt tcctgagcgt tcgcgactgg ggcgaggtgg tgaaggacga actgaagcgg
     1561 cttatcgcgc ccaccttgct gggcgtggcg gccggcgtcc tgctgtttgc ccgcctcgac
     1621 aaccacctgc tgctgcagtt gctgggcggg ttcctgatcg gctacgcgct ctattcgttc
     1681 gccgtgcaca ccttcggtct gccggaattc acctgctcgc cgcgctgggc ggctcccatc
     1741 ggcttcgccg gcgcgttcat cgacacgctg ttcggcgggg gcggcggcac cctggtggtg
     1801 atctatctcc acatgcgcaa ggtgggccgg gccggcttcc gcgccacggt ggcggcgttg
     1861 tggctcgccg aaatgatcgc gcgcatcgcc ggttacggcg ccgcgggcta ctactcggcc
     1921 gatatcctgc ttctgtgcgc gttgctgctg ccgctgatgg cagcggggac ctgggtgggg
     1981 gagcgcctcg gcaaccgcat cagccaggag gccttttcca ggctgctggc gctgctgctc
     2041 gcgctggccg gggtgagctt gctgttgaaa taacggaagg ggtttagagg aagaggaccg
     2101 cgggaaacgt cgcaccagcc agcaccagca cgagccactc caggttgttg cgcttcagcc
     2161 agccggtgaa atgcaggacc aggatgacta ttgccacgac gtaaaaagca tagaaagcca
     2221 tttacttctc cctgaatatc ccccggccgc aagaataaac ctaattggca cgcaacgaca
     2281 ggta
//
LOCUS       DBNGSG010000219         8337 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1767967, whole genome shotgun sequence.
ACCESSION   DBNGSG010000219 DBNGSG010000000
VERSION     DBNGSG010000219.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 8337)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8337)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8337
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1767967"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..584)
                     /locus_tag="AC158Q_05805"
     CDS             complement(180..584)
                     /locus_tag="AC158Q_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339322.1"
                     /translation="MKTGLLAFLLAAPALQFGGFAASASVEEAYRIVDRAEPLRCEIL
                     ALEERLKRAPEGSEEHAALAAEIGEAKARLKIHYQGTMFEYIAVMKLLPFEERKAVYR
                     YSSDVAGRCAAKACGGAPCSPAAEPAPVRQLK"
     gene            complement(762..1391)
                     /locus_tag="AC158Q_05810"
     CDS             complement(762..1391)
                     /locus_tag="AC158Q_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR3339323.1"
                     /translation="MDTASDTHRIASVEALEKLYGAPSGAAVDKEVDYLHPHYQKLIA
                     ASPFFVLATGGPDGMDASPRGDAPGFVAVEDEKTLLIPDRRGNNRVDSLRNIVANPRV
                     ALFFLVPGIGETLRVNGRAEIRTDPALLARFSVEGKLPRSVLVVRVDTVFFQCARAIF
                     RSQLWDPARHIARSSLPSTGTIVSDLTNSRFDGKQYDDGHYERLKSALY"
     gene            1626..2612
                     /locus_tag="AC158Q_05815"
     CDS             1626..2612
                     /locus_tag="AC158Q_05815"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3339324.1"
                     /translation="MRGARRSSICISLFTAAFLAAAALPAQAAETYPTKPIRVIVPFP
                     PGGSTDYNARAIHDKFSGLLGEQLVIDNRGGASGQIGTGIAMRSAPDGYTLLMHTVAF
                     VTSPILYSNAGYDPVKDFTAISLVSTVPMAISVHPSVPARSVKDLVDLAKSRPGGLHF
                     GSAGIGTNSHITGELFNLLGNTAIVPVHYKGGGPALAAVVSGEVQVAFSNVSQTVRMV
                     EAGRLRPLAVSSLKRSSALPNLPTVADAGLPGFEFVAWHVLAAPAGTPPAIVKTVSDK
                     IRATLADSVILQRFEKGGMDPVPSTPKETAAYLRNEQVKWRRVIKERNIKAQ"
     gene            complement(2750..3757)
                     /locus_tag="AC158Q_05820"
     CDS             complement(2750..3757)
                     /locus_tag="AC158Q_05820"
                     /inference="COORDINATES: protein motif:HMM:NF039362.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6159 family protein"
                     /protein_id="HIR3339325.1"
                     /translation="MKRCKKCNNLMPDDATVCIKCNPAAAKKIAPAPVKRVSPPPPPV
                     EPIKWTNAAGEKPGRIYGAWLLAQQSWKVLMLDKELLVFPLMSGIACFLVLASFFGAA
                     LAAGVTRSGSAAANEVVAWLLLFAYYFVNYFVIVFFNSALVACALVRFQGGNPTVEDG
                     MRAARARIPQIVAWALLAATVGVVLRAIQERVPAVGKFVIGLLGAAWTIATYFIVPLL
                     VVEKLGPFDAVKRSVTLMKQTWGEALASNVGIGAAVMVITVLFLVLAGSATVVLFGLT
                     GSTAVLVTCAVLIVACLVAVALISSALNTIILSALYLYAAEKKVPRAFDGIAQIAFAP
                     K"
     gene            complement(3855..4853)
                     /locus_tag="AC158Q_05825"
     CDS             complement(3855..4853)
                     /locus_tag="AC158Q_05825"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3339326.1"
                     /translation="MITIDIHAHIVPDSLWRAIDANKEWHGFRHEGGEGMGTVIGGGM
                     RTAFTSPKMRYTIKERLKDMDQQKVDVQVLSIHTPLIGYHLEPAQGLALAREVNDEIG
                     ATVRQSTGRLAGLATLPVQDVKIAIGELERAVTKLGLKGASLDTQVNGDQWDEPKFLP
                     FFKAAEQMGAVLFYHPQPQNNFLMQRIRRHGLFNSLGVILDDAIVAGVLICSGVLEKC
                     PDLKVCIAHGGGPTCYAMGRMDKNWHDRPPTRSTPLPPSAYQKKLYYDTVVGSEETLR
                     FLLDRVGADRVVLGSDWPFVPWHPSPVTWVQNLQSLTPEEKEKILWKNLAALLDIQ"
     gene            5067..5360
                     /locus_tag="AC158Q_05830"
     CDS             5067..5360
                     /locus_tag="AC158Q_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HIR3339327.1"
                     /translation="MRIIALRVLRDFWIRRPDAEIPLRSWFAAASRAEWSSPAEVKAA
                     YRNASFIANNRIVFNVKGNDYRLVVAVHYNRGMMFIRFVGTHREYDKIDASRI"
     gene            5373..5747
                     /locus_tag="AC158Q_05835"
     CDS             5373..5747
                     /locus_tag="AC158Q_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3339328.1"
                     /translation="MELRPIRTKREHQAALKEAEALWNAPEGSPEADRLEVLTLLIEA
                     DEREHFPIPDPDPIDFLLHVMEARELTRKDLEPFVGSRARVAEVLNRVRPLSLEMIRK
                     LSEGLDLPAEVLIRGYRVKRAA"
     gene            5783..7090
                     /locus_tag="AC158Q_05840"
     CDS             5783..7090
                     /locus_tag="AC158Q_05840"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR3339329.1"
                     /translation="MTLPARKPAASPIAMTAGEIAAAVAAGKTTCEAVARAYLERIEA
                     REPQVLAWQHHDPARVLAQARLLDQSGRRGPLIGVPFGIKDIIDTFDLPTEMGSPIYA
                     GHRPRGDAACVALTRKAGGVLMGKTVTTEFANRHPGKTRNPHDPARTPGGSSSGSAAA
                     VADGMAALAIGTQTTASTIRPASFCGVFGYRPTYGDLRCVGVKEAAGSLDTLGLIARS
                     IEDIALYRDVLLGESPVPLPAEARVPRIGFCRTPFWSRAEPGTQQMVEDAARRLAQAG
                     AQVRDVELPAEFAGIEDAHRWISSYEFARNFTWEIENHWEKISETLRNNRLKDGLSCS
                     FERYRKARDHAERCRELLTPVFDEHDVLLMPAATGEAPVGLDKTGDASFCLICTTTHA
                     PAVTLPVFKGPHGLPVGLQLMGRRNSDRALFAAGRWVYRTLSS"
     gene            7496..8011
                     /locus_tag="AC158Q_05845"
     CDS             7496..8011
                     /locus_tag="AC158Q_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HIR3339330.1"
                     /translation="MKLAAFEAVAAALQDAGVRYLVAGGIAVNAHGYLRLTYDVDLVI
                     QLQPANIQPAFKALQGLGYRPTVPVTAEQFADPAQRARWIREKGMQVLNFHSDRHRPF
                     TVDVFVSEPFDFDVEYEAAMRGEIAPGLTVRFVSIPTLIAMKQLAKRPRDLDDIEHLR
                     LIAEEKRKNGS"
     gene            8001..8183
                     /locus_tag="AC158Q_05850"
     CDS             8001..8183
                     /locus_tag="AC158Q_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339331.1"
                     /translation="MAPDADADWETATWEGSRRAQLRAALALTVRERLEALEELVRLS
                     QRLAEMPRSEPGSRHS"
BASE COUNT         1465 a         2804 c         2691 g         1377 t
ORIGIN      
        1 gcacagcgcg ttgtccacca gttgatcgac gatgatgcgc gcgtccccgc cgctgtatcc
       61 cacccgcgtc aacgcgcgct cgccgagcgc caccgcctcg gccacgctga gccgcacggt
      121 gccgggccgg tcggcgtcga gccatttctg tgacgagctg ctggcctgca ttcgatatcc
      181 tatttgagct gccgaacggg agccggctcc gccgccgggg aacagggcgc gccgccgcac
      241 gctttcgcgg cgcaacgccc ggcgacgtcg ctggagtagc ggtaaaccgc ctttctctcc
      301 tcgaaaggca gcaatttcat gacggcgatg tactcgaaca tcgtcccctg gtaatggatt
      361 ttcagccggg ccttcgcctc gccgatctcc gcggcgaggg cggcgtgttc ctcgctgccc
      421 tcgggagccc gcttcaagcg ctcctccagg gcgagaatct cgcagcgcaa cggctcggcg
      481 cggtcgacga tacggtaggc ctcctcgacg ctcgcgctag cggcaaagcc gccgaactgc
      541 aaggcaggag ccgccaggag gaaagcaagg agcccggttt tcaccttctg ctttcgccct
      601 gcgtcatttt gccgccgatg aacgccgata cacgccgata aaatcaggaa caatcagaca
      661 aggtcaaacc agatccggtc actcgtcacc gaggtaaagg tcccttttcc cttttgcctt
      721 gtgccttgtg ccctgtgcct tgtgccttgg cgcagcgggc gtcagtacag cgcgctcttc
      781 agccgctcgt agtggccgtc gtcgtactgc ttgccgtcga acctcgagtt cgtgaggtcg
      841 gagacgatgg tgccggtgct cggcagactc gagcgagcga tgtgccgggc gggatcccag
      901 agctgcgagc ggaagatcgc gcgcgcacac tggaagaaca ccgtgtccac gcgcacgacc
      961 agcaccgagc gcgggagctt gccctcgacg ctgaagcgcg cgagcagcgc cgggtcggtg
     1021 cggatctccg cgcggccgtt cacgcgcagc gtctcgccga tgccggggac caggaaaaac
     1081 agcgccacgc gcgggttggc gacgatgttg cgcaggctgt ccacgcggtt gttgccgcgc
     1141 cggtcgggaa tcagcagcgt cttctcgtcc tcgaccgcga cgaaccccgg cgcatcgccg
     1201 cgcggggacg cgtccatgcc atcgggcccg ccggtggcga gcacgaaaaa cggcgaggcc
     1261 gcgatcagct tctggtaatg cgggtggagg tagtcgacct ccttgtccac ggccgcgccg
     1321 gaaggcgcgc cgtagagctt ctccagcgcc tcgacgctgg cgatccgatg cgtgtcgctt
     1381 gcggtgtcca tgggcctctt ccaggatggg gatgaaggcg gaaggatgaa ggatgagccg
     1441 gctcttgtaa aacccgggaa cacgggccgg cgtcgcgcgt cgcctgtcac cgggccataa
     1501 aaaacgccgt gtattccggg ccttcccttc acccgtcgcg gagccctgta gcgcgaggcc
     1561 gtctattggg gaatactggg cgaaattgat cttgaagccg ctcaataacg acggaggaga
     1621 gaacaatgag aggagcccgc cgcagttcca tttgcatcag cctgtttacc gcggccttcc
     1681 tggccgccgc cgccctgccc gcgcaagcgg cggagacata cccgacgaag ccgatacgcg
     1741 tgatcgtgcc gttcccgccg ggcggcagca cggattacaa cgcgcgcgcg atccacgaca
     1801 agttctcggg cctgctcggg gagcagctcg tcatcgacaa ccgcggcggc gcctcgggcc
     1861 agatcggcac ggggatcgcc atgcgcagcg cccccgacgg ctacacgctg ctcatgcaca
     1921 cggtcgcgtt cgtcaccagc ccgatcctct acagcaacgc cggctacgat ccggtgaagg
     1981 atttcacggc gatctcgctg gtatccacgg tcccgatggc gatctcggtg catccgtcgg
     2041 tgccggcgcg ctcggtgaag gacctggtcg atctcgccaa gtcgcgcccg ggcgggctcc
     2101 atttcggctc cgcgggcatc ggcaccaatt cgcacatcac cggcgagctg ttcaacctgc
     2161 tcggcaatac cgccatcgtc ccggtccact acaagggcgg cggcccggcg ctcgccgcgg
     2221 tggtgagcgg cgaagtgcag gtcgccttct ccaacgtctc gcaaacggtg cgcatggtcg
     2281 aggcgggccg gctgcgcccc ctcgccgtca gcagcctcaa gcgctcgtcc gcgcttccga
     2341 acctgccgac ggtcgccgat gccggcctgc ccggcttcga gttcgtggcg tggcacgtcc
     2401 tcgcggcgcc cgcgggcacc ccgccggcga tcgtgaaaac ggtgagcgac aagatccgcg
     2461 ccaccctggc ggattccgtc atcctgcagc gcttcgagaa aggcgggatg gacccggtcc
     2521 ccagcacgcc gaaggaaacc gccgcctatc tcaggaacga gcaggtgaaa tggcggcggg
     2581 tgatcaagga gcggaacatc aaggcgcagt gacgggcttc gcctgacgga aaaagggaaa
     2641 agggaaaagg agcacagcct gacgggtgtc actttcacct ttcgactggc ttttccctcg
     2701 acacccgaca cccgtcacgc gtcaccgcca ccgaaggtgg cgctgaccgc tatttcggcg
     2761 cgaacgcgat ctgcgcgatg ccgtcgaacg cccgcggcac cttcttctcg gcggcgtaga
     2821 ggtagagcgc gctgaggatg atggtgttga gcgcgctgct gatcagcgcc acggccacca
     2881 ggcaggccac gatcaggacg gcgcaggtaa ccagcaccgc ggtgctcccg gtcagcccga
     2941 acagcaccac ggtggcggag ccggccagga ccaggaacag gaccgtgatc accatgacgg
     3001 ccgcgccgat ccccacgttg ctcgcgagcg cctcgcccca ggtctgtttc atgagcgtga
     3061 ccgagcgctt gacggcgtcg aagggcccga gcttctcgac caccagcagc ggcacgatga
     3121 aataggtcgc gatcgtccag gccgcgccca gcaacccgat cacgaacttg ccgacggccg
     3181 ggacgcgctc ctggatcgcc cgcaggacga cgcccaccgt cgcggccagc agtgcccagg
     3241 ccacgatctg gggaatgcgc gcgcgcgccg cgcgcatgcc gtcttcgacc gtcgggttcc
     3301 cgccctggaa acggaccagg gcgcaggcca ccagcgcaga gttgaaaaaa acgatgacga
     3361 aatagttgac gaagtaatag gcaaacagca gcagccacgc gaccacctcg ttggccgccg
     3421 ccgacccgct gcgggtgacg ccggcggcca gggcggcgcc gaagaagctg gcgagcacca
     3481 ggaagcacgc gatcccgctc atgagcggga acaccagcag ctccttgtcg agcatcagca
     3541 ccttccagct ctgctgggcg agcagccacg cgccgtagat ccgcccgggc ttttccccgg
     3601 ccgcattggt ccacttgatc ggctcgacgg gcggtggcgg cggcgacacc cgtttcacgg
     3661 gcgccggggc gatctttttc gcggccgcgg ggttgcactt gatgcacacc gtggcgtcgt
     3721 ccggcatcag gttgttgcac ttcttgcagc gtttcatggc tccctccttc gcggcaacgg
     3781 cgctagttta gcgtttccgc gattgcatga ctcctcaccc gccactgcgt cacagggcat
     3841 gggcccgtcc gccatcactg aatgtcgagc agcgccgcga gattcttcca gaggatcttc
     3901 tccttttcct ccggcgtcag cgactggagg ttctgcaccc acgtgaccgg cgacggatgc
     3961 cacggcacga acggccagtc gctgcccagc acgacccggt cggcgcccac gcggtcgagc
     4021 aggaagcgca gcgtttcctc gctgccgacc acggtatcgt agtagagctt cttctggtac
     4081 gcgctcggcg gcagcggcgt gctgcgcgtc ggcggccggt cgtgccagtt cttgtccatc
     4141 ctccccatgg cgtagcaggt gggcccgccg ccgtgggcga tgcagacctt gaggtccggg
     4201 cacttctcca ggacgccgct gcagatcagc actccggcca cgatggcgtc gtcgaggatc
     4261 acgccgaggc tgttgaagag cccgtggcgc cggatccgct gcatcaggaa gttgttctgc
     4321 ggctgcggat ggtagaagag gaccgcgccc atctgctcgg cggccttgaa gaatggcagg
     4381 aacttgggct cgtcccactg gtcgccgttc acctgcgtgt cgagcgacgc ccccttcagc
     4441 ccgagcttcg tcaccgcgcg ctccagttcc ccgatcgcga tcttcacgtc ctgcaccggc
     4501 agcgtggcga gcccggcgag gcgcccggtg ctctggcgca ccgtcgcgcc gatctcgtcg
     4561 ttcacttcgc gcgccagggc gagcccctgc gccggctcga ggtgatagcc gatcaaaggc
     4621 gtgtggatgg agagcacctg cacgtccact ttctgctggt ccatgtcctt caggcgctct
     4681 ttaatggtgt agcgcatctt gggggaggtg aacgccgtgc gcatgccgcc ccctatcacc
     4741 gtgcccatgc cctcgccacc ctcgtggcga aagccgtgcc actccttgtt cgcatcgatc
     4801 gccctccaca gcgagtccgg gacgatgtgg gcatggatgt ctatcgttat catgagcttc
     4861 cttgtaggag gggccgaggc cggctatgaa tgcgacagat aataaacgat tccagtcgcg
     4921 gtcattccgg aagtcgaaag gagaaaatga aaaaagcact gcttaacggc tgttacttgc
     4981 gacttcaggg aggcgccgcc cccagtcgca gccagcatac cggcgccaaa ggttgctccc
     5041 attttgggag catgctatag tgcggcatgc ggatcatcgc gctgcgggtg ctgcgggatt
     5101 tctggatcag gcgccccgat gcggaaatcc cgctgcgatc ctggtttgcg gcggcgagcc
     5161 gggccgaatg gagcagcccg gccgaagtca aggctgctta ccggaatgca agcttcatcg
     5221 cgaacaaccg tattgtattt aacgtaaagg gcaacgatta tcggctcgtc gtggcagtgc
     5281 actacaaccg gggcatgatg ttcatccgat tcgtgggaac gcatcgcgag tacgacaaaa
     5341 tcgacgccag caggatttga ggaccccgaa cgatggaact gcgccccatt cgaaccaagc
     5401 gagaacacca ggcagccctc aaggaagcgg aagcgctctg gaacgcgccg gaaggctcgc
     5461 cagaagccga ccggctcgag gtgctgacgc tgctcatcga agccgatgag cgcgagcact
     5521 tcccgatccc ggaccccgat ccaatcgatt tcctgctgca tgtcatggaa gcacgtgaac
     5581 tgacacgaaa ggatctggaa ccgtttgtcg gctcgcgcgc gcgggtcgcc gaagtgctga
     5641 atcgcgtgcg gccgctttcg ctggaaatga ttcgcaagct ctcggaaggc ctggatctcc
     5701 cggcggaagt ccttatccgc ggctaccgcg taaagcgcgc ggcgtgaatg cgcgaaacag
     5761 cgtgaacgat gaggatacga taatgaccct tcctgcccgc aaacccgccg cttcgcccat
     5821 cgcaatgacg gcgggcgaaa tcgccgccgc ggtcgccgcc ggcaagacga cgtgcgaagc
     5881 ggtcgcgcgc gcgtacctcg agcgcatcga ggcgcgcgag ccgcaggtgc tcgcgtggca
     5941 gcaccacgac cccgcgcggg tcctcgcgca ggcgcgcctg ctcgaccaga gcggccggcg
     6001 cggccccctc atcggcgtgc cgttcggcat caaggacatc atcgacacgt tcgacctgcc
     6061 gaccgagatg ggctcgccga tctacgcggg ccaccggccg cgcggcgacg cggcgtgcgt
     6121 cgcgctcacg cgcaaggcgg gcggcgtgct gatgggcaag accgtcacca ccgaattcgc
     6181 gaaccgccac ccgggcaaga cgcgcaaccc ccacgacccg gcgcgcacgc ccggcggctc
     6241 ctccagcggc tcggccgccg ccgtcgccga cggcatggcg gcgctggcca tcggcacgca
     6301 gaccaccgcc tcgacgatac gcccggcctc gttctgcggc gtgttcggct accggccgac
     6361 ctacggcgac ctgcgctgcg tcggagtcaa ggaggcggcg ggctcgctcg acaccctggg
     6421 gctcatcgcg cgctcgatcg aggacatcgc gctctaccgc gatgtcctgc tgggcgagag
     6481 ccccgtgccg ctgccggcgg aggcgcgcgt gccgcgcatc ggcttctgcc gcacgccgtt
     6541 ctggtcgcgc gccgagcccg gcacgcagca gatggtcgag gacgccgcgc gccggctcgc
     6601 gcaggcgggc gcgcaggtgc gtgacgtcga gctgcccgcc gaattcgcgg gcatcgagga
     6661 cgcgcaccgc tggatctcct cctacgagtt cgcgcgcaac ttcacctggg agatcgagaa
     6721 tcactgggag aagatcagcg agacgctgcg caacaatcgc ctgaaggacg ggctgtcgtg
     6781 cagtttcgag cgctaccgca aggcgcgcga tcacgccgag cgctgccgcg agctgctcac
     6841 gccggtcttc gacgagcacg acgtgctgct gatgccggcg gccaccggcg aggcgccggt
     6901 cgggctcgac aagacgggcg acgcgtcgtt ctgcctgatc tgcaccacca cgcacgcgcc
     6961 cgcggtcacg ctgccggtgt tcaagggccc gcacggcctg cccgtcggcc tgcaactcat
     7021 gggccggcgc aattccgacc gcgccctctt cgccgctgga cgctgggtgt atcgaacgct
     7081 tagttcataa ggaacctctg cataagtcat ccccgcgcag gcggggatcc agtaacgatt
     7141 gtttaacctg gattcccgcc tgcgcgggaa tgacgaaaaa cgacttttgc agagcatccg
     7201 taagcaaggc gaaagtcgaa agtaactgcc gtcaggcagg acccttctgc cttctgcctc
     7261 gttagcaacg gcgtgaatgg tgattggtga atagtgaatg gactcccgcg cagcgggaga
     7321 ctcatcactc gtcacccatc acccgtcact cgtcactggg tcttaaccct tccgcccttc
     7381 gcgtctcgcc tttcgccttc agttggcacc agccactatt tggcgcgata cttcatgcaa
     7441 tgaagcaggt catcgagacg gcgatgtccg ccggcgccca tgcaggaggc gctggttgaa
     7501 actcgccgct ttcgaggcgg tcgctgcggc gctgcaggat gccggcgttc gctacctggt
     7561 cgcgggtgga atcgcggtga acgcgcatgg ctacctgcgg ctgacatacg acgttgacct
     7621 ggtgatccag ttgcagcccg ccaacattca gccggctttc aaggcgctgc aagggctcgg
     7681 ctaccgcccc acggtgccgg tgacagccga gcaattcgcc gatccggccc agcgcgcgcg
     7741 ctggatccgg gaaaaaggaa tgcaggtgct caatttccac agtgaccgcc accggccgtt
     7801 caccgtggac gtcttcgttt cggagccgtt cgatttcgat gtcgagtacg aagccgccat
     7861 gaggggcgaa attgcaccgg gcctgacggt gcgtttcgtg tccataccca ccctgatcgc
     7921 gatgaagcag cttgccaagc gcccgcggga cttggatgat atcgagcatc tccggctgat
     7981 cgccgaggag aaacggaaaa atggctcctg acgccgacgc tgactgggaa accgcgacct
     8041 gggaaggcag ccgccgcgcc cagttgcgcg cggcgctcgc actgacggtc cgcgagagat
     8101 tggaagcgct ggaagaactg gtccggcttt cgcaacgcct ggcagagatg ccacgatcag
     8161 agccgggcag ccgtcattcg tgaacgtggc gaagatcgac tacgaatacg agacctccgg
     8221 ggagcgggtc cgggcctacc tgcgtgtccc cgagatagac cggctgcggt ggctcgagga
     8281 actttgggct ctcacctcca tggtgcggca ggcgccgacg acttactcac cggcgaa
//
LOCUS       DBNGSG010000220         5515 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1090074, whole genome shotgun sequence.
ACCESSION   DBNGSG010000220 DBNGSG010000000
VERSION     DBNGSG010000220.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5515)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5515)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5515
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1090074"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1396
                     /locus_tag="AC158Q_05855"
     CDS             <1..1396
                     /locus_tag="AC158Q_05855"
                     /inference="COORDINATES: protein motif:HMM:NF013225.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR3339332.1"
                     /translation="GSFVESGLFGASGVYPEQADETQTDGKLAGYARINGRDVCVVVN
                     DFTGKGASPSATNSKKIAHMKRTATERGTPLVVIGESTGARLPDAMGSRGMGLLLGND
                     ITQFRRMRETPMCAAALGPSFGSSTWLTCQADFSVMQKGAVVAVSSPRLVSMALGAKV
                     DPEDLGGWRLHAEVTGLIDQVVDKEEEVFDAIKRFLSYLPSHHMEAPPDAPVPAGSGA
                     DMDKVFSVLPESRTQVYDMKRIVRAVVDKDTFFEIKPRFGKSAAVGLARLGGRSVGIV
                     ANNPLHRGGALDADACRKIVDFLVLCDSFNVPIVLLVDTPGFQIGTEAERAGAPGKIM
                     NFMNAMTLVTVPHLSVIIRKSYGRAYVCMGGGRHSDDIAAWPTAEVSFMSPEFATKIV
                     HGVGVGEAGFEEKFAQIQKGADVWGLAEVYAAQAVIRPQDTRDYLIRMLGVHKLRATR
                     GVGQHLMRNWPTSY"
     gene            1515..2864
                     /locus_tag="AC158Q_05860"
     CDS             1515..2864
                     /locus_tag="AC158Q_05860"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR3339333.1"
                     /translation="MFNKVVVANRGAVAARVLRALNEMGVHSVAVYSEADAGAPYLEM
                     AGETHAIGPAPARESYLNMDALMAVVRQSRADGLHPGYGFLSENAAFAQKVTDAGARF
                     IGPSPRWIGLMGHKTRARELAAQHGMPMSKGSGVLPADRDAILSAARAIGFPVLVKPA
                     GGGGGIGMLPAKDEAELVAAVERSRSMAERGFGNAEVYLERLIGRPRHVEFQVLGDAH
                     GSAAHLYERDCSTQRRNQKVIEEAPAPGIPRERAAAVADQVAGIMRKLGYDNIGTVEM
                     LMGEDGSFSFLEMNTRLQVEHGVTEEVTGVDLVKAQIRSAFGEKLSAILPEKIEVSGH
                     AIQARVYAEDPKNFFPSPGNLRVFRPPRDNSVRVETGYAEGRDVTPHYDPMIAKVIVR
                     ADTRERAIDRLIEALAAFDIQGIKHNIPAVLAVLRSEAFRAGRVHTGLIPEVLKPGK"
     gene            3027..3722
                     /locus_tag="AC158Q_05865"
     CDS             3027..3722
                     /locus_tag="AC158Q_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HIR3339334.1"
                     /translation="MSEITEARKQGRVALDEAAGKQLLASFGVTVPRSIVVQDAAGAA
                     KACGELRPPLALKVISPDILHKSDAGGVKVGLKNAAEVEEVVRAMMKLPAIASARIDG
                     FLLEEMAPAGQEIVIGGLRDPQFGPLVMVGLGGIFVEVLKDVSFRICPVTRADAEDML
                     GELKGAALLDGARGRKPVSRPAIVDVLLKIGGEGGLLMTHARDIAEADINPLIVSETG
                     AVAVDARFILTKA"
     gene            3826..5268
                     /locus_tag="AC158Q_05870"
     CDS             3826..5268
                     /locus_tag="AC158Q_05870"
                     /inference="COORDINATES: protein motif:HMM:NF024994.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIR3339335.1"
                     /translation="MSDSVIERFKPLFEPKTVAVVGASTKGNALPNVFIRRIREFGYA
                     GEIYPIHPSAGAIDGLKAYRGLGDTPNPVDYAYIAIAAAQVPPLLRAARGRVRYAQVI
                     SSGFGEVDEGRELQDELAGAAQAGGMRLIGPNCLGIYTPRGRVTFAEIGPAETGPVGI
                     VSQSGGLGTDIIRRGLARGLKFSGLITVGNCADIGPNDLLEFYLADPQTRVIGMYIET
                     AKDGRRLFEILRDARAAKPVVILKGGRTRHGLAAAVSHTGSLAGDDRAWVALSKQTGC
                     VLAETLDEFIDILLVFQALAPQPRHPARRVVLFGNGGGASVLATDFFARLGLDVEVFG
                     KPAVDALAALKLPPGTSITNPVDAPVGTLQQDDGRVAEKILDIIYGSGRPEALVMHLN
                     LSAFVGRTKPEVLDNLVQAALRVQARYPGQAHFVLVLRSDGEPALEERKRAFRASAVA
                     LGIPVYDEMSNAGRSLAALAFHERFVASRK"
BASE COUNT          970 a         1731 c         1921 g          893 t
ORIGIN      
        1 gggcagcttc gtcgaatcgg ggttgttcgg cgcctcgggc gtctatcccg agcaggccga
       61 cgagacgcag accgacggca agctcgcagg ctacgccagg atcaacggac gcgacgtctg
      121 cgtcgtcgtc aacgacttca cggggaaggg cgcttccccc agcgccacca acagcaagaa
      181 gattgctcac atgaagcgca ccgccaccga gcgcggcacg ccgctcgtgg tgatcgggga
      241 atccaccggc gcgcggttgc ccgacgccat gggctcgcgc ggcatgggac tcctgctcgg
      301 caacgacatc acgcagttcc gccggatgcg cgaaacgccg atgtgcgccg ccgcgctggg
      361 accttcgttc ggctcctcga cctggctcac ctgccaggct gatttttcgg tgatgcagaa
      421 gggcgccgtc gtggccgtgt cgagcccccg cctggtatcg atggcgctgg gtgccaaggt
      481 ggatcccgag gacctcggcg gctggcgcct gcatgccgag gtgacgggac tcatcgacca
      541 ggtggtcgac aaggaagagg aggtcttcga cgccatcaag cgcttccttt cgtacctgcc
      601 gagccaccac atggaagcgc cgccggatgc gccggtgccc gccggttcgg gcgcggacat
      661 ggacaaagtg ttctcggtgc tgcccgagag ccgcacccag gtctacgaca tgaagcgcat
      721 cgtgcgcgcc gtcgtggaca aggacacgtt cttcgagatc aagccgcgct tcggcaagag
      781 cgccgccgtc ggcctcgcgc gcctcggcgg caggagcgtc ggcatcgtcg ccaacaaccc
      841 gctgcatcgc ggcggcgcgc tcgacgccga tgcctgccgc aagatcgtgg acttcctggt
      901 gctgtgcgac tcgttcaacg tgccgatcgt gctgctggtg gacaccccgg gcttccagat
      961 cggcaccgag gccgagcgcg cgggcgcgcc gggcaagatc atgaacttca tgaacgcgat
     1021 gacgctggtc acggtgccgc acctctcggt catcatccgc aagagctacg ggcgcgccta
     1081 cgtttgcatg ggcggcgggc gccattcgga cgacatcgcg gcgtggccga cggcggaggt
     1141 gagcttcatg agcccggagt tcgcgaccaa gatcgtgcac ggcgtgggcg tgggcgaggc
     1201 cggcttcgag gagaagttcg cgcagatcca gaagggcgcc gacgtatggg ggctcgcgga
     1261 agtctacgcg gcgcaggccg tgattcgccc gcaggacacg cgcgactatc tcatccgcat
     1321 gctgggggtg cacaagctgc gggcgacgag gggcgtaggg cagcacctga tgcgcaactg
     1381 gcccacttcc tattaaccaa aggcgtgaat ggtgaatggt gaatagtgaa tagacttcgc
     1441 gcgcacgccg cgcggcaagc cctgaaactc gttttgatcc attcaccatt cactattcac
     1501 cactcacgaa tttaatgttt aacaaagttg tggtggccaa ccgcggggcg gtggcggcgc
     1561 gggtgctgcg ggccttgaac gaaatgggcg tccattcggt cgccgtctat tccgaagccg
     1621 acgccggcgc gccgtacctg gagatggcgg gggagaccca tgcgatcggc ccggcgccgg
     1681 cgcgggagag ctacctcaat atggatgcgt tgatggcagt cgtgcgccag tcacgcgcgg
     1741 acgggctgca tcccggttac ggctttctct ccgagaacgc ggcattcgcg cagaaagtga
     1801 cggatgccgg cgcgcgcttc atcgggccgt cgccgcgctg gatcgggcta atgggccaca
     1861 agacccgcgc gcgcgagctg gcggcgcagc acggcatgcc gatgtcgaag ggctcgggcg
     1921 tgctgcctgc ggacagggac gcgatacttt cggcggcgcg cgcgatcggc ttcccggtgc
     1981 tggtgaagcc ggccggcggc ggcggcggca tcggcatgct gcccgcgaag gacgaggccg
     2041 agctcgtcgc ggcggtcgag cgctcgcgct cgatggccga gcgcggcttc ggcaatgccg
     2101 aagtctacct cgagcggctg atcgggcgcc cgcgccacgt cgagttccag gtgctgggcg
     2161 atgcgcacgg cagcgcggcg cacctctacg agcgcgactg ctcgacgcag cgccgcaacc
     2221 agaaagtgat cgaggaagcg ccggcgcccg gcatcccgcg cgagcgggcc gccgcggtcg
     2281 ccgaccaggt ggccggcatc atgcgcaagc tgggctacga caatatcggc acggtggaga
     2341 tgctgatggg ggaggacggc tcgttcagct tcctcgagat gaacacgcgc ctccaggtcg
     2401 agcacggcgt gaccgaggaa gtgaccgggg tggatctcgt gaaggcccag atccgctcgg
     2461 ccttcgggga aaagctttcc gccatcctgc cggagaaaat cgaggtgagc ggccacgcga
     2521 tccaggcgcg ggtctatgcg gaggacccga agaatttctt cccttcgccc ggcaacctca
     2581 gggtatttcg cccgccccgg gataacagcg tgcgcgtgga aacgggctac gcggaaggcc
     2641 gggacgtgac cccgcactac gacccgatga tcgccaaggt gatcgtgcgg gcggacacgc
     2701 gcgagcgggc gatcgaccgg ctgatcgagg cgctcgcggc gttcgacatc cagggcatca
     2761 agcacaacat cccggcggtg ctggcggtgc tgcgctcgga ggcgttccgg gcggggcggg
     2821 tgcacacggg gctgatccct gaagttctca aacccggaaa atagccgcca agacgccaag
     2881 gccgccaaga ggccgataat aaaaggcaaa aagaaagcga acacgtttga cgaaattcac
     2941 cacagggcaa aatcggaagc cgatttcgtc atttaatttt gattttcttg gcgttcttgg
     3001 cgtcttggcg gctaattaaa ggttttatgt ctgagatcac ggaagccaga aaacaggggc
     3061 gggtggcgct ggacgaagcc gcgggcaagc agctgctcgc gagttttggc gtcacagtgc
     3121 caaggtcgat cgtcgttcag gacgcggcgg gggcggcgaa agcctgcggt gaattgcgcc
     3181 cgccgctggc gctcaaggtc atttcgcccg acatcctgca caaaagcgac gccggaggcg
     3241 tgaaggtggg attgaagaac gccgcggaag tcgaagaggt ggtgcgcgcg atgatgaagc
     3301 tgccggcgat cgccagtgcg cgcatcgacg gctttctcct ggaagagatg gcgcccgccg
     3361 gccaggaaat cgtgatcggc ggtctgcgcg acccccagtt tggcccgctg gtcatggtgg
     3421 gcctgggcgg catcttcgtc gaagtcctga aagacgtgtc tttccgcatc tgcccggtca
     3481 cgcgcgccga cgccgaagac atgctgggcg agttgaaggg cgccgcgctg ctcgacggcg
     3541 cgcgggggcg caaacccgtc tcccggccgg ccatcgtgga tgtgctgctg aaaatcggcg
     3601 gcgagggcgg gctcctgatg acgcacgcgc gggatatcgc ggaagcggat attaatcccc
     3661 tgatagtgtc cgaaacgggc gccgtcgccg tggatgcgcg gtttattctt acaaaggcgt
     3721 gaatggtgaa tggagattgg tgaatggagc aagaccgttg cgctggctgc tttgcatcct
     3781 gggtccaatc accaatcact actcaccatt cacgaattga gcgagatgag cgatagcgta
     3841 atcgagcgct tcaagccgct gttcgagccg aaaaccgtcg cggtggtggg ggcgtcgacc
     3901 aaaggcaacg cgctgcccaa cgtgttcatt cgccgcatcc gcgaattcgg ctacgcgggc
     3961 gagatctacc cgatccaccc gtcggcgggc gcgatcgatg gcttgaaggc ttatcgcgga
     4021 ctgggcgaca cgccgaaccc ggtggactac gcctatatcg cgattgccgc ggcccaggtg
     4081 ccgccgctgc tcagggcggc gcgggggcgc gtgcgctacg cgcaggtgat ctcgagcggt
     4141 ttcggcgaag tggacgaagg ccgggaactg caggacgagc tggccggcgc ggcgcaggcg
     4201 ggcggcatgc gcctgatcgg ccccaactgc ctcggcatct acacgccgcg cgggcgcgtg
     4261 accttcgccg agatcggccc ggcggaaacc ggccccgtcg gcatcgtctc gcagagcggc
     4321 gggctcggca ccgacatcat ccggcgcggg ctcgcccgcg ggctcaagtt ctcggggctg
     4381 atcaccgtgg gcaactgcgc cgatatcggc cccaatgacc tgctcgagtt ctacctcgcc
     4441 gacccccaga ccagggtgat cgggatgtac atcgaaacgg cgaaggacgg gcgccggctg
     4501 ttcgaaatcc tgcgcgatgc gcgcgccgcc aagccggtcg ttatcctgaa aggcggccgt
     4561 acccggcacg ggctggcggc ggcggtctcg cacaccggct cgctcgccgg cgacgaccgc
     4621 gcgtgggtcg cgctctcgaa gcagaccgga tgcgtgctgg cggagacgct cgacgagttc
     4681 atcgacatcc tgctcgtttt tcaggcgctc gcgccgcagc cgcggcaccc ggcacggcgc
     4741 gtcgtgctgt tcggcaacgg cggcggcgca agcgtgctcg ccaccgattt cttcgcgcgg
     4801 ctgggcctcg acgtcgaagt gttcgggaag ccggcggtgg acgcgctcgc ggcgctgaag
     4861 ctcccgcccg gcaccagcat caccaatccg gtggatgcgc cggtgggcac gctgcagcag
     4921 gacgacgggc gcgtggcgga gaaaatcctc gacatcattt acggcagcgg ccggcccgag
     4981 gcgctggtaa tgcatctcaa cctctcggcc ttcgtggggc gcaccaagcc cgaggtgctc
     5041 gataacctgg tgcaggcggc gctgcgcgtg caggcgcgct atcccggcca ggcgcacttc
     5101 gtgctggtgc tgcggtccga cggtgaaccg gcgctggagg aacgcaagcg ggcgttccgg
     5161 gcgagcgcgg tcgccctcgg catcccggtc tatgatgaaa tgtcgaacgc aggccgctcg
     5221 ctcgcggcgc ttgcgttcca tgaacgcttc gttgcatcga ggaagtgact cgttgctcgt
     5281 ttttcgttcc tcgtccacgg tttgcaaaag gactgttaag ctttaaacca gaaacgagca
     5341 acgagtaacg agaaacgagc caccctttat gtattcctac gacttccacg cgacgggcta
     5401 cccgttccgg ctgtacagcg gcaaggacgc actcgagcgg ctgccggatg aagccgcgcg
     5461 tctgcgggcg agccgcgcct tcatcgtgtg cgggcgcacg gtatcgcgca agaca
//
LOCUS       DBNGSG010000221         6734 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2108454, whole genome shotgun sequence.
ACCESSION   DBNGSG010000221 DBNGSG010000000
VERSION     DBNGSG010000221.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6734)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6734)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6734
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2108454"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            225..1187
                     /locus_tag="AC158Q_05875"
     CDS             225..1187
                     /locus_tag="AC158Q_05875"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3339336.1"
                     /translation="MTRILTTLLLLLPLAFPAGAQEYPARPIRLIVAFAPGGVVDTAA
                     RVLTSKMTERLGWQFVIDNRTGGGGLIAGNIAAKAQPDGYTLLLAHTGEFAINQWLYK
                     KIPFDIERDFIPVTMVNDGPMLVVVSSQSPVSSFRELVAAAKAKPGQLSYGSPGSGAV
                     NHLATEWLAAAAGIKLLHVPYKGGAPAVTAVSQGEVLLTVAGVPGVLPHLNSGRVKVL
                     GVTTAKRWDFGKDWPTPQEGGIPGVDASIWTGLFAPRGVPKPVIDRLYRDVAATLKQA
                     DVRERYGTLGSEPVSMTPVQFQKRVKQDAQRYRKIVQDVGITPQ"
     gene            complement(1275..1859)
                     /locus_tag="AC158Q_05880"
     CDS             complement(1275..1859)
                     /locus_tag="AC158Q_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339337.1"
                     /translation="MAQDKGGGSKGGTSVVPTVGGAGKVSVESLVQRYLTIAGSEANA
                     RSLITGLRDGTEITLNGIIQEQVQVPVQTQVTETVMVPVRVPAPPPAPPGSFITVLQP
                     QTVTRTVTTYRTETITRSVTVVFTPPTGSMGLGNVDIALALTEAVLTQQSIAAPNPSQ
                     LQTALMDTNRGILQMRAKKMGWAEIAQALGFEVK"
     gene            complement(2175..3170)
                     /locus_tag="AC158Q_05885"
     CDS             complement(2175..3170)
                     /locus_tag="AC158Q_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339338.1"
                     /translation="MATGSRQKPPAGRKRAPAKRMPKKAAAGSRGIEPRDCRLDANSG
                     EIAEVSRRIEQEGGVVLGTYREPLGGHPVVFASLPIDKVEPTPFQRDLSEAHHKKLAG
                     VMDKTGLFLDPIIAITAPKEGFWTPNGRHRLAAMRRLGTKAVTALVIPNREIAWQILA
                     LNTEKAHNLKERSLEVIRIYKGLIEEDGARPESDFAFYLEEAALVTLGLCYEKKGNFA
                     GGAYAPILRKLETFSPEPLRKAIKEHEKNAEKVFELDELVSAAVAKLKAKGLMSPYLR
                     SFVVARINPLRWIQGDLPPLDDVLKTMRERAAKFNTEKIRQEDLASAGGAPDDEG"
     gene            complement(3201..4088)
                     /locus_tag="AC158Q_05890"
     CDS             complement(3201..4088)
                     /locus_tag="AC158Q_05890"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIR3339339.1"
                     /translation="MADTAFHGVFPYLVSPIDSSGTVKTDVLARLCDDLIRSGVHGLT
                     PLGSTGEFAYLSWPQRRSIVETVVAAAKGRVPVVAGVASTTLADAVMQAREFEGLGCN
                     GILAILEAYFPLSDEGVFEYFKAIAEAASIPIVLYTNPNFQRSDLSLPVIDRLSRIPN
                     IRYIKDASFNTGRLLSIINRVEGRMQVFAASAHIPACVMLIGGVGWMAGPACVAPRQS
                     VELYELCRRKDWSTAMERQRLLWTLNQAFARHNLAACIKGGLELQGYAVGAPLPPQSP
                     LSPEGVDEVRRALMAIGAL"
     gene            complement(4092..4244)
                     /locus_tag="AC158Q_05895"
     CDS             complement(4092..4244)
                     /locus_tag="AC158Q_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339340.1"
                     /translation="MIRKLPSGKYRLYSRHKNTRTGARRNLGTFTTRAAAERHERAVQ
                     FFKRRH"
     gene            complement(4253..4663)
                     /locus_tag="AC158Q_05900"
     CDS             complement(4253..4663)
                     /locus_tag="AC158Q_05900"
                     /inference="COORDINATES: protein motif:HMM:NF016701.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIR3339341.1"
                     /translation="MPQEPVKGSCLCGSVSFDFKPPFSAFRYCHCSRCRKATGSAHAS
                     NVFVAASQFAWSAGETLVNHFKLPGAKTFAVSFCTRCGSRVPHLIAERNEYLVPAGLL
                     DNDPVARPENAIFWGSKAPWHVETQTIPRFDERP"
     gene            complement(4707..4973)
                     /locus_tag="AC158Q_05905"
     CDS             complement(4707..4973)
                     /locus_tag="AC158Q_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339342.1"
                     /translation="MRTAIIILGGFLLLGICILVGRWIGGDNTRALAVAAQVFIPIWL
                     AAAGINMWIGVSQAGYTIIEELPIFILIFALPAAAAAFVWWKFS"
     gene            complement(4992..5909)
                     /locus_tag="AC158Q_05910"
     CDS             complement(4992..5909)
                     /locus_tag="AC158Q_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010814792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3339343.1"
                     /translation="MRDDKTVATVAAPTPLSDLTATFARDRIAIVALGTLSLILLAAV
                     FAPLVSPQNPYNLAQLDILDSKLAPGSSAGAGFTFWLGSDDQGRDMGSAILYGLRISL
                     GVGVVSTLFALAIGMAVGLLSAYAGGWIDNLIMRLVDLQLSFPSILIALILLAVVGQG
                     VDKIVIALITVQWAYYARTVRGAALAEQKKEYVDAARVMAFSSGRIVFRHILPNVLPP
                     LIVVATVQVAHAIALEATLSFLGLGMPITEPSLGLLISNGFAHLLSGRYWISFFPGVA
                     LLVTIVAINLVGDRLRDVLNPRLASHYGA"
     gene            complement(5906..>6734)
                     /locus_tag="AC158Q_05915"
     CDS             complement(5906..>6734)
                     /locus_tag="AC158Q_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008651130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3339344.1"
                     /translation="TAALGLDQPLWRQYWTFISGAFTGDLGKSFSANIPAVRLIFERM
                     PATFELALVAMIMAIALGIPLGLVAGLRPESWAGKTIMAGSILGFSLPTFWVGLMLIM
                     VFSVWLGWLPPTGRGQTVTVLGVPVSFLTWDGLAHLFMPALNLALFKLSLLIRLTRAG
                     TREALSQDYVRFARAKGLSMERVVRVHVLKNIMIPLVTVTGLEFGSLIAFAIVTETIF
                     AWPGMGKLLIDSINVLDRPVIVAYLLVIVFIFVVINLVVDLLYTALDPRVRLAGRPA"
BASE COUNT         1239 a         2103 c         2208 g         1184 t
ORIGIN      
        1 ctcgccgaac agcgcctcgc ggagctgacc gccgtcatcg actacaaccg cgcgctcgtg
       61 aatctgcagg cggtgcagac ggtgcccttg cagtagcaag ggaaaaggga aaagggaaaa
      121 gggaaaaggg aaaaggcaga aggcagaagg cggcaggcga tatgctgccg taacactttt
      181 gccttttgcc tcgtggtgac ttgtgccttc aaggaggggg agagatgacc cgcatactga
      241 cgactttatt gctgttgctg ccgctggcat tcccggcggg cgcccaggag tatcccgccc
      301 gacccatacg cctgatcgtg gcgtttgcgc cgggaggcgt ggtggacacc gcggcccgcg
      361 ttctgaccag caagatgacg gagcgcctgg gatggcagtt cgtgatagac aaccggaccg
      421 gcggcggcgg cctgatcgcc ggaaacatcg ccgcgaaggc gcagcccgat ggttacacgc
      481 tgctcctggc ccataccggc gagttcgcga tcaaccagtg gctgtacaag aaaatcccct
      541 tcgacatcga acgcgatttc attcccgtca ccatggtcaa tgacggcccg atgctggtgg
      601 tcgtcagcag ccagtcgcct gtgtcgagct tccgggaact ggtcgcggcg gccaaggcga
      661 agccgggaca gctgagctac ggttccccgg gctccggcgc ggtgaatcac ctggcgaccg
      721 agtggctggc cgcggccgcc gggatcaaat tgctgcacgt gccgtacaag ggcggcgcgc
      781 ccgccgtgac ggccgtctcg cagggcgagg tcctgctcac cgttgccggc gttcccggcg
      841 tgctgcctca tttgaactcg ggccgcgtca aggtgctcgg cgtgaccacg gcgaagcgct
      901 gggattttgg caaggactgg ccgacgccgc aggaaggggg catacccggg gtcgatgctt
      961 ccatctggac cggcctgttt gcgcccaggg gcgtgccgaa accggtcatc gaccgcctgt
     1021 acagggatgt cgccgcaacg ctcaagcagg ccgacgtgcg ggagcgctat ggcacgctcg
     1081 gatcggagcc ggtcagcatg acgccagtgc agttccagaa gcgcgtgaag caggacgcgc
     1141 agcgttacag gaaaatagtg caggacgtgg ggatcacgcc gcagtagcgg aacggggccg
     1201 cgaagccgca atgaaaaagg gacgaggctg acgccgcgtc cctttgttcg ccgtgcttgc
     1261 agcgggtttg ctggttactt gacctcgaat ccaagcgcct gcgcgatttc agcccagccc
     1321 attttcttcg cgcgcatctg caggatgccc cggttggtat ccatcagtgc ggtctgaagc
     1381 tgggaggggt tgggcgccgc tatgctttgc tgcgtcaaca ccgcttcggt cagcgccagc
     1441 gcgatgtcca cgttgccaag tcccatgctc ccggtgggcg gcgtgaagac gacggttacg
     1501 ctccgggtga tggtttccgt cctgtaggtg gtcacggtcc gggtgacggt ctggggctgc
     1561 aggacggtga taaaggagcc tggaggagcc ggcggcgggg ctggcactcg cacagggacc
     1621 atcacggttt cggtcacttg agtttgcacg ggcacctgca cctgttcctg gatgatgccg
     1681 ttcagcgtga tttcggtacc gtcgcgcagg ccggtgatga gcgatcttgc gttcgcttcc
     1741 gatccggcaa tcgtcagata acgctgcacc agcgattcga cggatacctt gccggcgccg
     1801 ccgaccgtcg gcacgaccga cgtgccgccc ttgctgccgc cgcctttgtc ttgtgccatc
     1861 gtcgcgcagg aaagcgcgag catgacaccc cctaaaagca gaactggcgc tgatttcttg
     1921 aacatttggc gctccttgtc ttaagcgagc cgtcctggct cgcgctaata ctatgacgga
     1981 ggaatcgggc cgagtagcct attccgtcac gcttactttc aacgggaaaa ccccactact
     2041 tcacgaatcc ggggctgaac tgacgccggt ggtcacagtt tgccatgggc cggcagactg
     2101 tgacggccag gggcaaccta acgccatgat cggtaaagag ttatggatgc agacgggcag
     2161 tcagcttgcc ggttttaccc ttcgtcatcc ggcgcgccac ccgccgatgc caggtcttcc
     2221 tgcctgatct tttcggtgtt gaatttcgcc gcgcgttcgc gcatcgtttt cagtacatca
     2281 tccagcggcg gcagatcgcc ctggatccag cgcaggggat tgatgcgcgc caccacgaag
     2341 cttcgcaggt aggggctcat tagccctttc gccttcagct tcgcgaccgc tgccgaaacc
     2401 agctcgtcga gctcgaatac tttctcggcg ttcttctcgt gctccttgat cgccttgcgc
     2461 agcggctccg gcgaaaacgt ctcgagcttg cgcagtatcg gcgcgtacgc tccgccggcg
     2521 aaattgccct ttttctcgta gcagaggccc agtgtcacca gcgctgcttc ttccaggtag
     2581 aaagcgaaat cggattccgg ccgcgcgccg tcctcttcga tcagcccctt gtagatgcgg
     2641 atgacctcga gcgaacgttc cttcaggtta tgagccttct cggtgttgag cgcgaggatc
     2701 tgccaggcga tctcgcgatt cggtatgacg agagcggtga ccgctttcgt gccaagccgc
     2761 cgcattgccg ccagccggtg gcggccgttg ggcgtccaga aaccttcctt cggggctgtg
     2821 atcgcgatga tcgggtcgag gaagaggccg gttttgtcca tcacgccggc gagtttcttg
     2881 tgatgcgcct cggacaggtc gcgctggaac ggcgtgggct cgaccttgtc gatcggcagg
     2941 gaagcgaaca ccacggggtg gccgcccagc ggctcgcggt aggtacccag cactacgccg
     3001 ccttcctgtt cgatacggcg gctcacctcg gcaatctcgc cggagttcgc atcgaggcgg
     3061 cagtccctcg gctcgatgcc gcgcgatccc gccgcggcct tcttcggcat gcgtttcgcg
     3121 ggcgcgcgct tgcgcccggc ggggggtttc tgcctggacc cggtggccat tgcaagttcc
     3181 gttaaccgat cccgcctatc ttacaacgct ccaatcgcca tcagcgcgcg ccggacttcg
     3241 tcgacgcctt caggcgaaag cggtgactgc ggcggcagcg gcgctccgac cgcgtagccc
     3301 tggagctcga gcccgccctt gatgcaggcg gcgaggttgt gccgggcgaa agcctggttg
     3361 agagtccata gcaggcgctg gcgttccatc gcggtgctcc agtccttgcg gcggcagagc
     3421 tcgtagagct caacgctctg cctcggcgcc acgcaggccg gcccggccat ccacccgacg
     3481 ccgccgatca gcatcacgca cgccggaatg tgcgcggagg cggcgaagac ctgcatccgg
     3541 ccctcgaccc ggttgatgat ggaaagcaga cggccggtgt tgaaggatgc gtccttgatg
     3601 taacggatgt tcgggatccg gctcaggcgg tcgatgacgg gcaggctcag gtccgaacgc
     3661 tggaagttgg gattggtata cagcactatc gggatcgatg ccgcctcggc gatcgccttg
     3721 aagtattcga acacgccttc gtcggagagc ggaaagtagg cttccaggat ggcgagaatg
     3781 ccgttgcagc ccaggccctc gaactcgcgc gcctgcatga ccgcgtcggc gagggtggtc
     3841 gaggccacgc ccgcgacgac cgggaccctg cctttcgccg ccgcgaccac ggtttccacg
     3901 atgctccgac gttgcggcca cgacaggtag gcaaattcgc cggtcgaacc gagcggagtg
     3961 agcccgtgca cgccggaccg gatcaagtca tcgcacagcc gcgccaggac gtccgttttc
     4021 accgtgcccg aggagtcaat cggggaaacg aggtacggaa atacgccgtg gaacgcggta
     4081 tcagccatga ctcaatgtcg tcgcttgaag aactgcaccg cccgctcgtg ccgctcggcc
     4141 gccgcgcggg tggtgaacgt gccgaggttg cgccgcgcgc cagtgcgcgt gttcttgtgg
     4201 cgggaataca gacggtactt ccccgacggg agcttgcgga tcatcgcggc cgtcacggac
     4261 gttcgtcgaa tctcgggatt gtctgcgtct cgacgtgcca aggcgctttc gagccccaga
     4321 atatggcgtt ttcgggacgc gcgaccgggt cgttgtccag caatccggcg gggaccaggt
     4381 actcgttcct ctcggcgatc aggtgcggca ccctggagcc gcaacgcgtg cagaaggaga
     4441 ccgcaaaagt cttcgcgccg ggcagcttga aatgattgac cagcgtctcg ccagccgacc
     4501 acgcaaactg gcttgccgcc acgaatacgt tggacgcatg cgcgctgccg gtcgctttgc
     4561 ggcaacggct gcaatgacag tagcggaacg ccgaaaaagg cggcttaaaa tcgaagctca
     4621 ccgatccgca caggcagctg cctttcaccg gttcctgggg catcgtgatt tcctttcttg
     4681 attcgcggag catgcgtcaa ttcttctcag gagaatttcc accagacgaa cgccgcagcc
     4741 gcggcgggca gggcgaagat caggataaag atcggcagct cttcaatgat cgtatagccg
     4801 gcctgggata cgcctatcca catattgatt ccggcggcgg cgagccagat cgggatgaag
     4861 acctgggcgg cgacggccag cgctcttgta ttgtcaccgc caatccatcg cccgacaaga
     4921 atgcagatgc cgagcagcag gaagcctccg aggatgatga tggctgtgcg cacggatgta
     4981 ctccccttgc attatgcccc gtagtggctg gccagcctcg ggttcagcac gtcccgcagc
     5041 cggtcgccca ccaggttgat cgcaacgatc gtgacaagca gcgccacgcc ggggaagaag
     5101 ctgatccagt aacgccccga gagcaggtgg gcgaagccgt tggaaatcag caggccgagc
     5161 gagggctcgg tgatcggcat gccgaggccg aggaaggaga gggtggcttc gagcgcgatg
     5221 gcgtgcgcga cctgcacggt cgcgacgacg atcagcggcg gcagcacgtt cggcaggatg
     5281 tgccggaata cgatgcgccc gctgctgaac gccatgacgc gcgcggcgtc cacgtattcc
     5341 ttcttctgct cggccagcgc cgccccgcgc accgtgcgcg catagtaggc ccactggacg
     5401 gtgatgagcg cgatcacgat cttgtccacg ccttgcccga ccaccgcgag caggatcagc
     5461 gcgatcagga tcgaggggaa ggagagctgg agatccacca gccgcatgat caggttgtcg
     5521 atccagccgc cggcgtaagc cgagaggagg ccgaccgcca tgccgatcgc gagcgcgaac
     5581 agcgtggaca cgacgcccac gcccaggctg atccgcaagc cgtagaggat cgccgaaccc
     5641 atgtcccgtc cctggtcgtc gctgccgagc cagaaggtga atcctgcgcc cgcgctcgac
     5701 cccggcgcga gcttggaatc cagtatgtcg agctgcgcga ggttgtacgg gttctgcggc
     5761 gaaaccagcg gggcgaaaac tgcagccagc aggatcagcg ataacgtgcc aagcgcgacg
     5821 attgcgatgc gatcccgcgc gaaggtcgcg gtgagatcgc tcaacggggt cggagcggca
     5881 accgtcgcca ctgtcttgtc atccctcacg ccgggcgtcc cgccagcctg acgcgcgggt
     5941 ccagcgccgt gtaaagcagg tccacgacca ggttgatgac gacgaagatg aatacgatga
     6001 ccagcaggta ggcgacgatg acggggcgat caagcacgtt gatcgagtcg atcaggagtt
     6061 ttcccatccc cggccacgcg aagatggtct cggtgacgat ggcgaaagcg atgagcgagc
     6121 cgaactcgag ccccgtcacg gtcacgagcg ggatcatgat gttcttcagc acgtgtacgc
     6181 gcaccacgcg ctccatggaa agccccttcg cgcgcgcgaa gcggacatag tcctgcgaca
     6241 atgcttcgcg cgtgccggcg cgggtgaggc ggatcagcag cgagagcttg aacaaggcca
     6301 ggttcagcgc cggcataaag aggtgcgcga gcccgtccca cgtgagaaag ctcaccggca
     6361 cgccgagcac cgtcacggtc tgtccccgcc cggtcggcgg cagccagccg agccacaccg
     6421 agaacaccat gatgagcatc aatccgaccc agaaggtcgg cagcgagaag ccgaggatcg
     6481 agcctgccat gatggttttg cctgcccagg attcgggcct gagccccgcg acgagcccga
     6541 gcgggatgcc gagcgcgatc gccattatca tcgcgacgag ggccagctcg aacgtcgccg
     6601 gcatgcgctc gaagattagc ctgaccgccg gaatgttggc ggagaacgac ttgcccaggt
     6661 cgcccgtgaa tgcgcccgag atgaatgtcc agtactgccg ccacagcggc tggtcgagcc
     6721 cgagcgcggc ggtc
//
LOCUS       DBNGSG010000222         4201 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3128335, whole genome shotgun sequence.
ACCESSION   DBNGSG010000222 DBNGSG010000000
VERSION     DBNGSG010000222.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4201)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3128335"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..856
                     /locus_tag="AC158Q_05920"
     CDS             179..856
                     /locus_tag="AC158Q_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3339345.1"
                     /translation="MRVLIIEDDPAIAANLYDFLEARGHVVDAAADGVTGLHLAITGG
                     FDAIVLDLGLPGMDGGTLCRKLRGEAHDDTPVLMLTARDTLDDKLEGFDQGADDYLVK
                     PFALKEVEARLAALHKRRAGRVTARPLTVGDLFFDPASVAVRFHGRDVRLPPKCMRLL
                     QVLMTQPNRVFSRKELEAEVWGEGQETSDTLRSHMHVLRRELVQAGGRDPIENVHGLG
                     YRLVPNA"
     gene            849..2108
                     /locus_tag="AC158Q_05925"
     CDS             849..2108
                     /locus_tag="AC158Q_05925"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3339346.1"
                     /translation="MRSLGLRHRVALGLALACLLIVGALGITLYAASEDMEESLISQI
                     VAEEMDYLIQRHRENREYQPQRSSNLQSYIVRDPSAQKDLPAFLREIAPGRHEFYLDK
                     DEFHVLVREDGGIRYYVAYEVGLHEQREQEFRLLVLLSVLTAVLASLALGYWLSGVLV
                     RQVVELAHRVGTLKPGQHRVELARPGQDLEVSMLARAFEGYQARIESMIRREQEFTSN
                     ASHELRTPLTAIGTSCEILLGDPALGEKSRARVEWISAAAARMSEQLQTLLFLARGQA
                     LGEIEPVALAECVAEAVEPYHGEFTRKNLSLEVDIARDAVLQLNYPALRLVVGNLVRN
                     AVHYTERGFVKVSYAGKRLAVADSGQGIEAEHLARVFERFFRAGDRADGSGLGLAIVK
                     RICEQYGWKIEIDSAPGRGSTFSITFP"
     gene            2332..2982
                     /locus_tag="AC158Q_05930"
     CDS             2332..2982
                     /locus_tag="AC158Q_05930"
                     /inference="COORDINATES: protein motif:HMM:NF023682.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermostable hemolysin"
                     /protein_id="HIR3339347.1"
                     /translation="MTLNAAPLAGPNAATTGNPGADAKTRRAHWSFSRAGARERPQVE
                     SFIRRGFERAYGARLTRLMPTLMALRCGAETAAACGLRPAAAEQLFLEAYLDRPVETA
                     LSRAAGMQLDRADIVEVGNLVIARAGCARRLIFHLTTHLHAAGPAWVVFTAVPALRNS
                     FRRLGIPLVTLGAADGNRLPAAARAEWGSYYEQAPIVTAVSVTAAFHAVCEAACTR"
     gene            2970..>4201
                     /locus_tag="AC158Q_05935"
     CDS             2970..>4201
                     /locus_tag="AC158Q_05935"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3339348.1"
                     /translation="MHPVIAAIGRHAAAHPRRPALTDGAREISYLTLNRAIADTGAEM
                     RMRTSKPVALCLDNSPEWIIADLALLATRLPCVPLPSFFSAQQQAHAAADAGVECVVT
                     DQPERCIVRLRSAGLPAVRDAEFHLAGRRLACIRIGVQPAAALPRGTVKITYTSGTTG
                     NPKGVCLGGDALAKVARSLASACGLGAGDRHLSLLPLATLLENLGVYASLVAGACCAL
                     PPLEEIGIAGASGVQPAALLDAMARHRATTAIMVPQMLQAVVGRIERGAPVPSNLRFL
                     AVGGAVVTPALLQRAATLGLPVFEGYGLSECGSVVSLNTPQANRRGSAGRPLPHCTLS
                     FGRDGEIYAAGATLLGYHGAAAPAGNAWPTGDIGHLDDEGYLHITGRKKDCFISSYGR
                     NVSPEWIEAALTLEPAIA"
BASE COUNT          687 a         1464 c         1370 g          680 t
ORIGIN      
        1 cctacaagat ccagttccag ccgatgcagg gcctcgtcag cggccgctgg cagccgatcg
       61 cgcctccgcg aaaagggtga ctcgtctctc gtttctcgtt tctcgttccg ggcagttcca
      121 gccgaaaaat ccggccaaag acgagtaacg agcaacgaat aacgaggaac ggagtactgt
      181 gcgcgtactg atcatcgaag acgatccggc gattgccgcc aacctctacg acttcctcga
      241 ggcgcgcggg cacgtggtgg atgccgccgc cgacggcgtg acggggctgc atctcgccat
      301 tacgggcgga ttcgatgcca tcgtgctcga cctcgggctt cccggcatgg acgggggcac
      361 gctctgccgc aagctgcgcg gagaagccca cgacgacacc ccggtgctga tgctgacggc
      421 gcgcgacacc ctggatgaca agctcgaagg cttcgaccag ggagccgacg actatctcgt
      481 caaacccttc gccctcaagg aggtcgaagc gcgtctcgca gccctgcaca agcggcgcgc
      541 agggcgcgtc acggcgcggc cgctcaccgt cggcgatctg tttttcgatc cggccagcgt
      601 ggccgtgcgg ttccacggca gggacgtcag gctcccgccc aaatgcatgc gactgctgca
      661 ggtcctgatg acccagccca accgcgtttt cagccgcaag gaactcgaag cggaggtctg
      721 gggcgagggg caggagacga gcgacacgct gcgcagccac atgcacgtgc tgcgccgcga
      781 gcttgtccag gccggcggcc gcgatccgat cgagaacgtg cacggactcg gctaccgcct
      841 ggtgccgaat gcgtagcctc ggcctgcgcc accgggtcgc gctcggcctc gccctggcct
      901 gcctgctgat cgtgggtgcg ctcggtatca cactctacgc cgcctccgag gatatggagg
      961 aatcgctgat cagccagatc gtcgcagagg aaatggacta cctgatccag cgccaccgcg
     1021 agaaccgcga gtaccagccg cagcgcagct ccaacctcca gagctacatc gtgcgcgacc
     1081 cgtccgcgca gaaagacctt cccgcgtttc taagggaaat cgcgccaggc cggcacgagt
     1141 tttacctcga caaggacgaa ttccacgtgc tcgtccggga agacggcggc atccgttact
     1201 acgtcgctta cgaagtcggc ctgcacgagc agagagagca ggaattcagg ctgctggtgc
     1261 tgctctcggt gctgaccgcc gtgctcgcgt cgctcgcgct cggctactgg ctctccggcg
     1321 tgctggtgcg gcaggtcgtg gaactcgccc accgcgtcgg cacgctcaag ccgggacagc
     1381 accgcgtcga actggcgcgc ccgggccagg acctggaagt ctcgatgctg gctcgagcct
     1441 tcgagggcta ccaggcgcgt atcgagagca tgatccgccg cgaacaggaa ttcacctcga
     1501 acgcgagcca cgaactgcgc acgccgctca cggccatcgg aaccagctgc gagatcctgc
     1561 tgggcgaccc ggcactgggc gagaaatcgc gcgcgcgggt ggaatggatc agcgcagcgg
     1621 ccgcgcgcat gtcggagcag ttgcagactt tgctcttcct cgcccgcggc caggcactcg
     1681 gagaaatcga gccggtcgcg cttgcggagt gcgtggcgga agcggtcgag ccatatcacg
     1741 gagagttcac ccgcaagaac ctgtcgctcg aggtggacat cgcgcgcgat gccgtgctcc
     1801 agctgaacta tccggcattg cgcctggtag tcggcaatct tgtgcgcaac gccgtccatt
     1861 acaccgagcg cggcttcgtc aaggtgagct atgccggcaa gcgccttgcg gtagccgact
     1921 ccggccaggg catcgaggcc gagcacctgg cccgcgtctt cgagcgtttc ttccgcgccg
     1981 gggacagggc ggacggcagc gggctgggac tcgccatcgt caagcgcatc tgcgagcagt
     2041 acggctggaa aatcgagatt gacagcgcgc cggggcgggg ctcgacgttc agcattacat
     2101 ttccctaagg aacctccgca cgagcggccg ccggcgcgac gagcctcggc gaggctgccc
     2161 ctgacgtgag gttgcacgaa gggactgcgt cccctcgttc cgggcgtccc caacccgcct
     2221 acttcacaat tccttcacgg cctgaccacg ggtttttgac gccgtccggg ctaacctcgt
     2281 tgtcgatttc aacagacgcg gccggccagt cccggtgcgg aatagaatcc gatgacgctg
     2341 aacgccgcgc ctctggcagg tcctaacgcc gccacgacgg gaaatcccgg tgctgacgca
     2401 aaaacccgcc gcgctcactg gtcattctca cgcgctggcg cccgcgagcg cccccaggtc
     2461 gagtcattca tacgtcgcgg cttcgaacgc gcatacggcg cccgcctgac gcgattgatg
     2521 ccgacgctga tggccctgcg ctgcggggcc gaaaccgccg cggcctgcgg cctgcgcccc
     2581 gccgcggccg aacaactttt cctcgaggcc tacctcgacc ggccagtcga gaccgcgctc
     2641 tcccgcgccg cgggaatgca gctggatcgc gcggatatag tcgaagtcgg caatctcgtc
     2701 atcgcgcggg ccggctgcgc ccgtcgcctg atatttcacc tgacgacgca tctccacgcg
     2761 gccgggccgg cgtgggtggt cttcaccgcc gtgccggcgc tgcgcaacag tttcaggcgc
     2821 ctcggcatac cgctggtcac gttgggagcc gccgacggca atcgcctgcc cgccgcggcg
     2881 cgcgcggaat ggggcagcta ttacgagcag gcgccgatcg ttacggcggt atccgttaca
     2941 gcggccttcc atgccgtgtg cgaggccgca tgcacccggt aatcgccgcg atcgggcgtc
     3001 acgcggcggc gcatccccgt cgtcccgcac tgaccgacgg cgcgcgggag atcagctatt
     3061 tgacgctcaa ccgtgccatc gccgacaccg gcgccgaaat gcggatgcgg acttcgaagc
     3121 cggtcgcgct gtgcctcgat aactcgccgg aatggatcat cgccgatctg gcactgctcg
     3181 ccacgcggtt gccgtgtgtg ccgctgccct ccttcttctc cgcccagcag caggcgcacg
     3241 ccgccgcgga tgcgggagtg gaatgcgtgg tcaccgacca gccggaacgc tgcatcgtgc
     3301 ggctgcggtc ggctggcctg cccgcggttc gggatgccga atttcacctg gcaggccgcc
     3361 gcctcgcctg catccgcatc ggtgtgcagc cagccgccgc cctgccgcgc ggcaccgtga
     3421 agatcaccta cacctccggc accaccggca acccgaaggg cgtttgcctc ggcggcgacg
     3481 ctctcgctaa agtcgcccgt tcgcttgcgt cggcttgcgg attgggcgcg ggcgaccggc
     3541 acctgagcct gctgccgctg gcgacgctgc tcgagaacct cggggtttac gcgtccctcg
     3601 tcgccggcgc gtgctgcgcg ctgccgccgc tggaggaaat cggcatcgcg ggcgcatccg
     3661 gcgtgcagcc ggcggcgctg ctcgacgcga tggcccgcca ccgcgcgacg accgccatca
     3721 tggtgccgca aatgctgcag gccgtcgtcg ggcgcatcga acgcggcgcg ccggttccct
     3781 ccaacctgcg ttttctggcc gttggcgggg ccgtggtaac gccggctctg ctacagcgcg
     3841 ccgcgacgct cgggctgccg gtattcgaag gttacggcct ctcggaatgc ggctccgtcg
     3901 tatcgctcaa tacgcctcaa gccaatcggc gcggcagcgc cggacgcccg ctgccccact
     3961 gcacgctgtc tttcgggcgc gacggggaga tctacgcggc gggagcgacg ctgctgggct
     4021 atcacggcgc ggccgcgcct gccggcaatg cctggccgac tggtgatatc ggacatctcg
     4081 atgacgaggg ctatctccac atcaccggcc gcaagaagga ttgcttcatc agctcgtacg
     4141 gccgcaatgt ttcacccgaa tggatcgagg ccgcgctcac gctggagccg gccatcgcgc
     4201 a
//
LOCUS       DBNGSG010000223         4995 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_581096, whole genome shotgun sequence.
ACCESSION   DBNGSG010000223 DBNGSG010000000
VERSION     DBNGSG010000223.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4995)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_581096"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="AC158Q_05940"
     CDS             complement(<1..379)
                     /locus_tag="AC158Q_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011901949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3339349.1"
                     /translation="MVAAIQVEQVSKAYGALRALDGVSLSIERGEFFGLLGPNGAGKT
                     TLINVIAGLARPDAGSASVMAADVTSDYRRARRMLGVVPQELVFDPFFTVRETLRIQS
                     GYFGIRRNDAWIDEVMQHLDLTAK"
     gene            complement(468..749)
                     /locus_tag="AC158Q_05945"
     CDS             complement(468..749)
                     /locus_tag="AC158Q_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR3339350.1"
                     /translation="MIACEDGRCTVQGPITLGSVVSLLAQGNGLFTAPQVTLDLAGVT
                     EVDSSALSLLLEWRREAGRNGRAIRYLNLPANLKSLADLYGVTELLGEA"
     gene            complement(746..1387)
                     /locus_tag="AC158Q_05950"
     CDS             complement(746..1387)
                     /locus_tag="AC158Q_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaC/ttg2D family ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR3339351.1"
                     /translation="MMIKRFLLGMLAACFLAGTPARAQEIVAPDVLAKNVTDEVLAVL
                     RADKDIQSGNTKKVVDLVETKVLPHFEFARMTRLAVGPNWRKATADQQKALINEFRSL
                     LVQTYAATLAAYRNQTIEYRPLRMQPADTEVVVKSLIQQSGGKPVTVDYKMEKTATGW
                     KVYDMVVADLSLVQNYRGSFETEVRKGGIDGLIKALVDKNQQLAAGQVAAEKK"
     gene            complement(1421..1996)
                     /gene="mlaD"
                     /locus_tag="AC158Q_05955"
     CDS             complement(1421..1996)
                     /gene="mlaD"
                     /locus_tag="AC158Q_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004542689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HIR3339352.1"
                     /translation="MDRTTLDVWVGIFVTAGIAALFILALKVGNASTTFNVGESYRIS
                     AEFENIGGLKVRAPVKSAGVVVGRVSDIQFDGKKFVARVTINVDKRYEFPKDTSVSIL
                     TSGLLGEQYLGLEAGGDTALLKEGDTITLTQSAVVLEKLIGQFLFSKAAEGGNKESGS
                     KEGGSKESGSKEAGSKESGSKESGSKEAGSK"
     gene            complement(2001..2783)
                     /gene="mlaE"
                     /locus_tag="AC158Q_05960"
     CDS             complement(2001..2783)
                     /gene="mlaE"
                     /locus_tag="AC158Q_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HIR3339353.1"
                     /translation="MKGLRGVGHQVVNGVWRLGYATRFFLVTLTRSATSFRRFRGLTF
                     REIYFAGVLSLIIIIVSGQFVGMVLALQGYETLQKYGSSEAVGTLVALSLVRELGPVV
                     AALLFASRAGSAMTAEIGLMKATEQISAMEMMAVDPIARVIGPRFWGGVISMPLLAAI
                     FSAMGVFGGYLIAVVLIGVDEGAFWSQMQNAVDFRQDIVNGIIKSVVFGVAVTAIALF
                     EGYDAPPTAEGVSGATTRTVVTSSLAILGLDFILTAFMFKGV"
     gene            complement(2791..3609)
                     /locus_tag="AC158Q_05965"
     CDS             complement(2791..3609)
                     /locus_tag="AC158Q_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018060161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3339354.1"
                     /translation="MENLVEIRDVNFSYDARPVLKGINMTVPRGKIVAIMGLSGCGKT
                     TILRLIAGVLRPRAGSVKVAGRAVNELDRDRLYEMRSKMGMLYQFGALFTDLSAFDNV
                     AFQMREHTSLPESMIEDLVLMKLNAVGLRGARDMMPSELSGGMARRVALARTIALDPT
                     LIMYDEPFTGLDPISMGVISNLIRQLNNALGATCIIVTHDVQESLKIVDYVYFMSDGV
                     VVAEGTPEDIRNSKAPFVHQFVWGEMDGPMPFHYPGAPYAQDLELSPGLRTARA"
     gene            3780..4667
                     /locus_tag="AC158Q_05970"
     CDS             3780..4667
                     /locus_tag="AC158Q_05970"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3339355.1"
                     /translation="MKVFVTGSGSHLAAALLPRLLARPEIERVTGVDLDPPHFQHDRF
                     DPVRLDIRSPRLDDLLTGHDALVHLACVRRQGSLSDREMFDINVNGSHKAFHAARRAG
                     IRRFVHLSSAAVYGSGVHLSEAAPFDPLPQLLYARHQAHLETLLQIEFPDCVRLRPSL
                     ILGPHARPALKRLLRLPFYVRTPAPYSLLQCVHEDDVARAVLLALERDVRGPFNLAVE
                     DNFNLREVARRRHRLVLPLPPFAARAGLALARRWLDWDGELAWIEGLTRTLLVNCRRA
                     AVVLGWRSAHSAASVLAQS"
BASE COUNT          863 a         1702 c         1508 g          922 t
ORIGIN      
        1 ccttggcggt gaggtcgagg tgctgcatca cctcgtcgat ccaggcgtcg ttgcggcgga
       61 ttccgaagta gcccgactgg atgcggagcg tctcgcgcac cgtgaagaac gggtcgaaga
      121 cgagctcctg cggcacgacg cccagcatgc gacgcgcgcg ccggtagtcg gacgtgacgt
      181 ccgccgccat tacggacgcc gagccggcat cggggcgcgc gagccccgcg attacgttga
      241 tcagggtggt cttgcccgcg ccgttcggcc cgagcaggcc gaaaaattcg ccgcgctcga
      301 tcgagaggct taccccgtcg agcgcgcgca gcgcgccgta agccttcgag acctgctcga
      361 cctggattgc cgcaaccatt cagcgcgtga tgagtgatag gtgatgggtg atgggcgaaa
      421 ccacccaaga gctactgccc attacccgtt actcattacc gagtcgctca cgcttcgccg
      481 agcaactcgg tgacgccgta cagatcggca aggctcttga ggttggccgg caggttgagg
      541 tagcggatcg cgcggccatt gcgcccggcc tcgcgccgcc attcgagcag cagggagagc
      601 gcggaggaat ccacctccgt gacgccggcg agatcgaggg tcacctgcgg cgccgtgaac
      661 agcccattac cctgggcgag cagcgacacc acgctcccca gggtgatcgg cccctgcacc
      721 gtgcagcggc cgtcctcaca cgcgatcatt ttttttccgc cgccacctgg ccggccgcaa
      781 gctgctggtt cttgtcgacc agtgccttga tcaacccgtc gatgcccccc ttgcgcacct
      841 cggtctcgaa gctgccgcgg tagttctgca ccaggctgag atccgccacc accatgtcgt
      901 acaccttcca cccggtggcg gtcttctcca tcttgtaatc caccgtgacc ggcttgccgc
      961 ccgactgctg gatcagcgac ttgacgacga cttccgtgtc ggcgggctgc atgcgcagcg
     1021 gccggtattc gatggtctgg ttgcgatagg cggcaagcgt cgcggcgtag gtttgcacca
     1081 gcagtgaccg gaattcgttg atcagcgctt tctgctgatc ggcggtcgcc ttgcgccagt
     1141 tcgggcccac ggccagacgc gtcatccggg cgaactcgaa gtgcggcagg acttttgtct
     1201 cgaccaggtc caccaccttc ttcgtgttgc ccgactggat gtccttgtcc gcgcgcagca
     1261 ccgccagcac ttcgtccgtc acgttcttcg ccagcacgtc cggtgccacg atctcctgcg
     1321 cgcgcgccgg tgtacccgcc agaaaacagg cggccagcat tcccagcaag aatctcttta
     1381 tcatcattgg ggtccttacc tcgtcacttc gcacaattag ttacttgctc ccggcttcct
     1441 tgctcccgct ttctttgctg ccgctttcct tgctcccggc ttccttgctc ccgctttctt
     1501 tgctgccgcc ttccttgctc ccgctctcct tgttcccgcc ttccgcggcc ttgctgaaga
     1561 gaaactgccc gatcagcttc tcgagcacca cggccgattg cgtaagcgtg atcgtatcgc
     1621 cctccttcag cagcgcggtg tccccgcccg cctcgagacc gaggtactgc tcgcccagca
     1681 ggccggaggt gagtattgag accgacgtat ccttgggaaa ttcatagcgt ttgtcgacat
     1741 tgattgtcac ccgcgcaacg aacttcttgc cgtcgaactg gatatcgctc acccggccaa
     1801 ccaccacgcc ggcgcttttc accggcgcgc gcaccttcag ccccccgatg ttttcgaact
     1861 cggcggagat gcgatagctc tcgcccacgt tgaacgtcgt gctggcattg cccaccttga
     1921 gggcgaggat aaacagggcg gcaatcccgg cggtcacgaa gatgccgacc cacacatcga
     1981 gagtggttct gtccatgcgc ctagactccc ttgaacatga aagcagtgag gatgaaatcg
     2041 agcccgagta tggcgagcga cgaggtgacc accgtgcgcg tggtcgcccc cgaaacgccc
     2101 tccgccgtcg gcggggcgtc gtagccttcg aacagcgcaa tggcggtcac cgccacgccg
     2161 aacaccacgc tcttgatgat gccgttcacg atatcctgcc ggaaatccac ggcattctgc
     2221 atctgcgacc agaacgcgcc ctcgtctacc ccgatcagca ccaccgcgat caggtagccg
     2281 ccgaacacgc ccattgcgct gaaaatcgct gcgagcagcg gcatggagat cacgccgccc
     2341 cagaagcgcg ggccgatcac gcgcgcgatc gggtccaccg ccatcatctc catcgcggag
     2401 atctgctcgg tcgccttcat caggccgatt tccgcggtca tggccgagcc ggccctgctt
     2461 gcgaacaaca gcgcggcgac caccggcccc agctcgcgca cgagagacag cgcgaccagc
     2521 gtgccgacgg cttccgaaga gccgtacttc tgcagcgtct cgtagccctg cagcgccagc
     2581 accatgccca cgaactggcc cgagacgatg atgatgatga gcgagagcac gccggcgaaa
     2641 taaatttccc ggaacgtcag gccgcgaaag cggcgaaaac tggtggccga tctcgtcagg
     2701 gtgaccagga aaaaccgcgt cgcgtaaccg agccgccaga cgccgttcac cacctggtga
     2761 ccgacaccgc gcagcccttt cacgacgcgc tcaggcacgc gccgtcctca gccccgggct
     2821 cagttccaaa tcctgcgcgt acggcgcccc ggggtaatgg aacggcatcg ggccgtccat
     2881 ttcgccccac acgaactggt gcacgaacgg ggccttcgaa ttgcggatgt cctcgggcgt
     2941 gccttccgca acgacgacgc cgtccgacat gaaatacacg tagtccacga tcttgagcga
     3001 ttcctgcacg tcgtgcgtga cgatgatgca ggtcgcgccc agcgcgttgt tcagctggcg
     3061 gatcaggttg ctgatgacgc ccatcgagat cgggtcgaga ccggtgaacg gctcgtcgta
     3121 catgatcagc gtcggatcga gcgcgatcgt ccgggcgagc gccacgcggc gcgccatgcc
     3181 gcccgatagc tcggaaggca tcatgtcacg ggcgccgcgc agccccacgg cattcagctt
     3241 catcaacacg aggtcctcga tcatggattc cggcaggctg gtgtgctcgc gcatctggaa
     3301 cgcgacgttg tcgaacgccg agagatcggt gaacaaggcg ccgaactggt agagcatgcc
     3361 cattttgctg cgcatctcgt agagccggtc gcggtcgagc tcgttgacgg cgcggcccgc
     3421 cactttcacc gacccggccc tgggcctcag cacgccagca atgaggcgca ggatggtcgt
     3481 cttcccgcag cccgacaggc ccatgatcgc gacgatcttg ccgcgcggca ccgtcatgtt
     3541 gatgcctttg agcaccggac gggcatcgta cgaaaagttg acgtccctga tttcaacgag
     3601 gttttccatc gatccggcca agggcgccaa aaaagcgtca attttaaact acccgttccg
     3661 gcgcaacaat gacggattgg acaccagctg cggcccgaca ttcgagggaa acggcgattt
     3721 ttaagtgacg tccggcacaa tgcggctgca cgcgccggga tagaattcaa cccagttgca
     3781 tgaaagtctt cgtcaccggg tccggctcgc acctcgcggc agcgctgctg ccgcggctcc
     3841 tcgcgcgccc ggaaatcgag cgggtgaccg gcgtcgatct cgacccgccg catttccagc
     3901 acgaccgctt cgacccggtg cggctcgata tccgcagccc gcggctcgat gacctgctca
     3961 cggggcacga tgcgctggtt catctcgcct gcgtgcgacg gcagggcagc ctgtccgacc
     4021 gggaaatgtt cgacatcaac gtcaacggca gccacaaggc gttccacgcc gcgcggcgcg
     4081 ccggcatccg gcgcttcgtc cacctgtcga gcgccgcggt ttacggaagc ggcgtgcatt
     4141 tgagcgaggc agcgccgttc gatccgctgc cgcaactgct ctacgcccgc caccaggcgc
     4201 acctggagac gctgttgcag atcgaatttc ccgattgcgt gcggctgcgg ccgagcctga
     4261 tcctgggacc ccatgcgcgg cccgcgttga agcggctgct gcgcctgccg ttctacgtgc
     4321 gcacgccggc accgtactcc ctgctgcaat gcgtgcacga ggatgacgta gcgcgggccg
     4381 tcctgctcgc gctcgagagg gacgtgcgcg gcccgttcaa cctggccgtc gaggacaact
     4441 tcaatctccg cgaggtcgcc aggcgtcgcc accgcctcgt tctgccgctg ccgcccttcg
     4501 cggcgcgcgc gggcctcgcc ctggcccggc gctggctcga ctgggacgga gagctcgcgt
     4561 ggatcgaggg gctcacccgc acgttgctcg tgaattgccg gcgcgccgcc gtcgtgctcg
     4621 gctggcgcag cgcgcacagc gcggcttccg tgctcgcgca gagctgaatt tagccgccaa
     4681 ggcgccaaga aatccaaata tcgatcctcg gatcgtctta cagttgagtc gatggcaccg
     4741 caccgcatgt tgaacggcgg ccaattcgtc cgttcgaaca ggcggagtta aaccggcgcg
     4801 gcacgtccgg atttaaccgt gcttggcggc tattttgatt cgtcgtttac tattcatcgt
     4861 cttttcggat aacgtatccg catgagcgac atcgcgctgt gcttcgagcg ggtcacgaag
     4921 agctacggcg gcgtgcccgc attgagcgct ttctcgctcg aggtgaagcg cggagcgttc
     4981 ttcgggctgg tcggc
//
LOCUS       DBNGSG010000224         2008 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1091080, whole genome shotgun sequence.
ACCESSION   DBNGSG010000224 DBNGSG010000000
VERSION     DBNGSG010000224.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1091080"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..366)
                     /locus_tag="AC158Q_05975"
     CDS             complement(169..366)
                     /locus_tag="AC158Q_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HIR3339356.1"
                     /translation="MKIVVILFLVFIVASLFSALYFLVRDKGSGERTVKALTLRVTLS
                     VVLFALLMLGYYFGFIPGRLT"
     gene            417..1154
                     /locus_tag="AC158Q_05980"
     CDS             417..1154
                     /locus_tag="AC158Q_05980"
                     /inference="COORDINATES: protein motif:HMM:NF014193.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial respiratory chain
                     complex IV assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HIR3339357.1"
                     /translation="MPARYRFQPRVVTTLAAAAGIALTLALANWQLNRAHEKEGRAAQ
                     LETLAKDPPVPLAASEARVEDIEWRQVTARGRFEPRYGVLIDNRIHRGVAGYHVVMPL
                     EIRSGGGDAARYVLVNRGWIAGNPARARLPEVKTPAGTVEVSGLAVTPSRRFLELAPG
                     AAEGKIWQNLTLERYREAFPLVLHPVLIQQESPLDDGLVREWDPPNLGVDKHYGYAFQ
                     WLALALTILVFYLVTHVRRTPEKTQPG"
     gene            1117..1692
                     /locus_tag="AC158Q_05985"
     CDS             1117..1692
                     /locus_tag="AC158Q_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR3339358.1"
                     /translation="MSDAPPKKRSLGSLWLIVALTVAPVAASYLLYYFWPPGRTVNYG
                     ELIEPRPLPDPKMTLVGGAPFELSQLRGKWLLVSVDSGRCDAHCDRKLLYLRQLRLTQ
                     GKNTERVERIWLVSDEITPRDEAVSQYQGTWVVHADAGLLQLFPARGALADHIYLVDP
                     LGNLMMRFPRDPDPRLMIKDLARLLKASQIG"
     gene            1689..>2008
                     /locus_tag="AC158Q_05990"
     CDS             1689..>2008
                     /locus_tag="AC158Q_05990"
                     /EC_number="1.17.99.9"
                     /inference="COORDINATES: protein motif:HMM:NF014665.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HIR3339359.1"
                     /translation="MKLYRPLVFCAVALTFIVIVAGAYVRLEDAGLGCPDWPGCYGQL
                     IGVPDEAHEVASAERSFPGKTVDTARAWKEMSHRYLAGTLGLLILAIAMIAWLKRGET
                     GRPP"
BASE COUNT          355 a          623 c          664 g          366 t
ORIGIN      
        1 acaacaacaa ggggggaccc cgtcagtagg acaacggacg ccgcgcccga tgtcgcaatc
       61 ggcgcggcgc tttttttagt gctgagtttt tagtgttgag tgttgagttt tgagtgggtg
      121 agttcaggtt ttcactcaac actaagcact caacacttaa cactaccgtc aggtgagcct
      181 gccggggatg aagccgaagt agtatccgag catgagcagc gcgaacagaa ccaccgacag
      241 cgtgacccgc agcgtcagcg cctttaccgt gcgctcgccg cttcccttgt cgcgaaccag
      301 gaaatagagc gcggagaaca ggctggcgac gatgaagacg aggaacagga tgacgacgat
      361 tttcacgcgc ggaccggcgg gctgctgaat tgtccttagc atataccttt acccacttgc
      421 ccgcccgcta ccgattccag ccccgcgtgg tcaccacgct cgccgccgcg gcggggatcg
      481 cgttgacgct ggcgcttgcc aactggcagt tgaaccgcgc gcacgagaag gaaggacggg
      541 cggcgcagct cgagaccctg gccaaggacc ctccggtccc ccttgcggcg tccgaggcga
      601 gagtggagga catcgaatgg cgacaggtta cggcgcgcgg acgtttcgag ccgcgctacg
      661 gcgtgctcat cgacaaccgg attcaccgcg gcgtggcggg ttaccacgtg gtgatgccgc
      721 tcgaaatccg cagcggcggt ggtgacgccg cccgctatgt gctggtgaat cgcggctgga
      781 ttgcgggaaa cccggcgcgc gcgcgcctgc cggaggtcaa gacgccggcg ggtactgtcg
      841 aggtctccgg gctcgcggtc acgccgagcc ggcgctttct ggaacttgcc cccggggcag
      901 cggaaggcaa gatctggcag aacctgacgc tcgagcgcta tcgcgaggcg tttccgctcg
      961 tgctgcatcc ggtgttgatc cagcaggaaa gcccgctcga cgacggcctc gtgcgcgagt
     1021 gggatccgcc caacctcggc gtcgacaagc attacggcta cgcgttccag tggctcgcgc
     1081 tggcgcttac gatactcgtg ttctacctgg tgacccatgt cagacgcacc cccgaaaaaa
     1141 cgcagcctgg gtagcctctg gctgatcgtc gcgctgaccg tggcgccggt cgccgcttcg
     1201 tacctgcttt attatttctg gccgcccggg cgcaccgtga attacggcga gctgatcgag
     1261 ccgcgcccgt tgccggaccc gaaaatgacg ctcgtgggcg gggcgccctt tgaactctcg
     1321 cagctgcggg gaaaatggct tctggtaagc gtggactcgg ggcgctgcga tgcgcattgc
     1381 gacaggaagc tcctctacct gcggcaactg cggctcacgc agggaaagaa cacggagcgc
     1441 gtggaacgga tctggctggt cagcgacgag atcacgccgc gcgatgaggc ggtctcgcag
     1501 taccagggga cctgggtcgt gcatgccgac gccgggttgc tgcagctttt tccagcccgg
     1561 ggcgcgcttg ccgatcatat ctacctggtg gatcctctcg gcaacctgat gatgcgcttc
     1621 ccgcgcgatc ccgatccccg cctcatgatc aaggaccttg cacgcctgct gaaagcatcg
     1681 caaatcggat gaaattgtat cgacctctgg ttttctgtgc cgtcgcgctc accttcatcg
     1741 tcatcgtggc gggcgcgtac gtgcggctgg aggacgcggg gctgggctgt cccgactggc
     1801 cgggttgcta tggacaattg attggagtgc ccgacgaggc gcacgaggtt gccagcgccg
     1861 agcggagctt cccgggcaag accgtggata cggcgcgcgc gtggaaggag atgtcccacc
     1921 gctatcttgc cggcacgctg ggcctgctca tactcgcgat cgcgatgatc gcatggttga
     1981 agcgcggcga gaccggccgc ccgccctg
//
LOCUS       DBNGSG010000225         4107 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4147745, whole genome shotgun sequence.
ACCESSION   DBNGSG010000225 DBNGSG010000000
VERSION     DBNGSG010000225.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4107)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4147745"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="AC158Q_05995"
     CDS             complement(<1..552)
                     /locus_tag="AC158Q_05995"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR3339360.1"
                     /translation="MTPRPENLAPRARPWRPAPAIRLSLLLHVAGVAALVLQPSHWPW
                     VLGGLATNHLLLIAAVLWPRGRVLGPNLVRLPSAAAARNEVSLNFDDGPDPEVTPRVL
                     DLLDRHRARASFFCIGEKAAAFPELVKEIARRGHSVENHTHRHAGIFAFYGPGRLRRE
                     VETAQTAIAAIAGRAPEFFRAPAG"
     gene            complement(549..1220)
                     /locus_tag="AC158Q_06000"
     CDS             complement(549..1220)
                     /locus_tag="AC158Q_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3339361.1"
                     /translation="MDAIHDPAPSAGADRIALVMLPGARDRAQDLVEHGFVQALRTRD
                     LAVDAVAVDARFDHYLEHSIVARLEEDIIAPLRRKGLARIWLMGISLGSTGALSYARE
                     HSGAVEGMILLAPFLGTRGLIAEITQAGGLRHWQPGTIAPQDDERRLLAWLRQYRAGD
                     PALPRICLGYGTEDRYAPASLMLAQQLPERQVMTIPGGHDWPTWLKLWERWLDSDALA
                     SPGKP"
     gene            complement(1204..3540)
                     /locus_tag="AC158Q_06005"
     CDS             complement(1204..3540)
                     /locus_tag="AC158Q_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012356288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HIR3339362.1"
                     /translation="MSSIPSRWAVGAWLVFVAACIAVIARATITTDISAFLPRSPTPA
                     QSILVDQLRDGVVSRLILIGIEGAAPETLARASKQLAAVLRRQDGFASVNNGENAGFA
                     GDRDFLWRNRYLLSRAVTPDRFSPDGLRARLEENLQLLASPAGMLLRRILPNDPTGEL
                     IHLLESMEGQARPASRGGVWFSRDGSRALLVAQTRAAGYDIDAQERALGAIRGAFAQL
                     DGGTEAKLLLSGPGVFSVHSRAAIKGDALRFSLIALLLIGAMTLAFYRSPRVLALSFV
                     PVATGAAAGVAAVSLGFGSVHGITLGFGVTLIGEGVDYAIFLFTRAAPGISPYHTLDR
                     VWPTLRLGVLTSLCGFSAMLFSGFTGLAQLGLFSIAGLIVAVAVTRWVLPALMPAGFT
                     AAAVTALAPVAMAALRRAPPLRYPLLAVIVLAAASLALHRGPLWSDNLSSLSPVPRAD
                     QLLDQQLRRDLGAPDLRYIVVVSAADGETALRSAESAAAVLNDAARAGMLEGYDSPAT
                     YLPSRLAQRARQDALPAPAELRRNLREALKGLPYRPEVFEPFLKDAAKARSMQLLSHD
                     SLRGTSVALKLDTLLVRRGPGWAAMLPLAGVTDAAGIAREIGALSGARAVLLDLKGES
                     DRLYADYRREALTHSALGAVAIVALLFLGLRSPRRVFDVLAPLAAAVVITTGVLATMG
                     QLSIFHLVGLLLVVAVGSNYSLFFDRQVASGPDRDRTVVSLLFACASTIIGFGVLSFS
                     KVPVLNSIGVTVGIGAILALVCSAILSRSMDPIHGRDT"
     gene            complement(3764..>4107)
                     /locus_tag="AC158Q_06010"
     CDS             complement(3764..>4107)
                     /locus_tag="AC158Q_06010"
                     /inference="COORDINATES: protein motif:HMM:NF039988.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LolA-related protein"
                     /protein_id="HIR3339363.1"
                     /translation="ESLVLEGDRLILENKDRNQRRTFALQDQPVIWAFVESIRSTLAG
                     DLATLNRFYQVALDGGERQWRLTLKPAEPDMQGMVSEIRIGGSRNRVNTIEIIETQGD
                     RSVMTITADVP"
BASE COUNT          746 a         1404 c         1372 g          585 t
ORIGIN      
        1 tcccgccggc gcgcgaaaga actcgggcgc ccgccccgcg atggcggcga tggccgtctg
       61 ggctgtttcc acttcacgtc gcaagcgccc gggcccgtag aaagcgaaga tgccggcatg
      121 gcggtgagtg tggttctcca cgctgtggcc gcggcgcgcg atttccttta cgagttcggg
      181 aaacgcagcc gccttctcgc cgatacagaa gaagctcgcg cgcgcccgat ggcgatcgag
      241 caagtcgagc acgcgcggcg tcacctccgg gtcgggccca tcgtcgaagt tgaggctgac
      301 ctcgttgcgc gctgcagcgg cggatggcag gcgcacgaga ttcgggccca gcacgcgccc
      361 gcgcggccac aatacggccg cgatcagcag cagatgattc gtggcgagac cccccagcac
      421 ccacggccag tgcgatggct ggagcacgag cgccgccacg cccgcgacat gcagcagcag
      481 ggatagtctg atggcgggcg ccggccgcca tgggcgggcc ctgggcgcga gattctcggg
      541 gcgcggtgtc atggctttcc cggtgatgcc aatgcatcgc tatccagcca gcgttcccac
      601 aacttcagcc aggttggcca atcatgcccg cccggtatgg tcatgacctg ccgttcggga
      661 agttgctgcg caagcatgag gctagctggc gcatatcggt cctcggtgcc gtatcccagg
      721 cagatgcgcg gcagcgccgg gtcgccggcg cggtattgcc gcagccaggc cagaagccgg
      781 cgctcgtcgt cctgcggcgc aatggtcccg ggctgccagt gccgcaaccc tcccgcttgc
      841 gtgatttcgg cgataagccc gcgcgtgccg agaaacggcg cgagcaggat catgccctcg
      901 acggcgccgg aatgctcgcg cgcgtaggac agcgcgccgg tgctgcccag cgagatgccc
      961 atcagccaga tccgggcgag cccctttctc cgcaacggcg cgatgatgtc ctcctcgagc
     1021 cgcgcgacga tgctgtgctc gagatagtgg tcgaaacgag cgtccacggc gacggcatcg
     1081 accgccagat cgcgggtgcg cagcgcttgc acgaagccgt gctcgaccag gtcctgcgcc
     1141 cggtcgcgcg cgcccggcag catcaccagc gcgatgcggt ctgcgcccgc actgggcgcc
     1201 gggtcatgta tcgcgtccat ggatgggatc catgctgcgg ctcaggatcg cggaacagac
     1261 gagcgccagg atggcgccga tgccgacggt gaccccgatc gagttcagga ccggcacttt
     1321 cgaaaacgac agcacgccga agccgatgat cgtggacgcg caggcaaaca gcagcgacac
     1381 gaccgtgcgg tcgcggtccg gccccgaggc cacctgccgg tcgaagaaca gggaatagtt
     1441 ggagccaacc gccaccacca gcagcagccc cacgaggtga aaaatggaca gctggcccat
     1501 cgtcgccagc acgccggtcg tgatgacgac cgccgccgcg agcggcgcca gcacgtcgaa
     1561 tacccggcgc ggcgagcgca gcccgagaaa cagcagcgcc acgatggcca cggcgcccag
     1621 agccgagtgc gtcagcgcct cgcggcggta gtccgcgtag agccggtcgg attcgccttt
     1681 caggtccagc agcacggccc gcgcgccgga aagcgcaccg atttcgcggg cgatacccgc
     1741 ggcgtcggtc acgcccgcca gcggcagcat ggccgcccag ccgggaccgc ggcggaccag
     1801 caaggtatcg agcttgagcg cgacgctcgt gccccgcagg ctgtcgtggc tcagaagctg
     1861 cattgaccgc gctttcgcag cgtccttcag gaaaggctcg aatacttcgg gacgatacgg
     1921 caggcccttg agcgcctcgc gcaggtttct gcgcagctcg gccggagccg gcagcgcgtc
     1981 ctggcgcgcg cgctgcgcca gccggctcgg caggtacgtc gccggggaat cgtacccttc
     2041 cagcatgccc gcgcgcgctg cgtcattcag gaccgccgcg gccgactccg ccgagcgcaa
     2101 cgcggtttcc ccgtccgcgg cgctaacgac cacgatatag cgcaggtcgg gcgcgccgag
     2161 gtcccggcgc agctgctggt cgagcagctg atcggccctc ggcaccggag agaggctcga
     2221 gaggttgtcg ctccacagcg gtccgcgatg gagcgcaagc gacgcggcag ccagcacgat
     2281 cacggcgagc agcgggtagc gcagcggcgg cgcgcggcgc agcgcggcca tcgccacggg
     2341 cgcaagcgcc gtcaccgcgg cggccgtgaa tccggccggc atcagcgccg gcagcaccca
     2401 gcgcgtcacg gccactgcga cgatgagccc ggcgatggaa aacagcccga gctgggcgag
     2461 cccggtgaaa cccgagaaca gcatcgcgct gaagccgcac agggacgtca gcacgcccag
     2521 ccgcagcgtc ggccagacgc ggtcgagcgt gtggtacggc gagatgcccg gcgccgcgcg
     2581 cgtgaaaagg aaaatcgcat aatccacgcc ttcaccgatc agcgtgacgc cgaagcccag
     2641 cgtgatgccg tgcaccgagc cgaaacccag gctgaccgcg gccacgccgg cagccgcccc
     2701 ggtcgcaacc ggcacgaagc tcaaggcgag gacccgcggc gaacggtaaa aagccagggt
     2761 catggccccg atcaacaaca gggcgatcag cgaaaaccgc aacgcatcgc ccttgatggc
     2821 agcgcgcgag tggaccgaga acacgccggg ccccgacagc agcaatttcg cttccgtgcc
     2881 gccgtccaat tgcgcgaagg cgccccggat cgctcccaat gcgcgctcct gggcgtcgat
     2941 gtcgtatccc gcggcgcggg tctgcgcaac gagcagcgcc cggctgccgt cgcgggagaa
     3001 ccatacgccg ccccggctcg cgggccgcgc ctgcccttcc atgctctcca ggaggtgaat
     3061 cagctcgccg gtcggatcgt tgggcaggat gcgccgcaac agcatgccgg cgggcgaggc
     3121 cagcagttgc aggttttcct ccaggcgcgc gcgcagcccg tccggcgaaa accggtccgg
     3181 agtgaccgcc cggctcagca ggtagcggtt gcgccacagg aaatcccggt ccccggcgaa
     3241 gccggcgttc tccccgttgt tgacggaggc gaagccgtcc tgccggcgca ataccgcggc
     3301 gagctgcttg ctcgcccgtg cgagcgtttc gggcgcggca ccctcgatgc cgatcaggat
     3361 gaggcgcgac accacgccgt cgcgcagctg atcgaccagt atgctctgtg cgggcgtggg
     3421 cgagcgcggc aggaacgcgg aaatgtcggt cgtgatcgtg gcccgggcaa tcaccgcgat
     3481 gcacgccgcg acgaacacga gccaggcgcc caccgcccat cgcgaaggaa tggagctcat
     3541 ctcaggcaat caggcgcacg caaccagaat ggacaggatt tacgggatcg aaaccaaaga
     3601 tcaaaaacaa atagacagga ttaacaagat taacaggatt caaacaacaa cataaaaaaa
     3661 gcgttctgat ttgttcttga tctaatcctg taaatcttgt aaatcctgtc tattaatggg
     3721 gttgacgggt tcactgtcta ttgtcaatcc tgtctattgc ttttcacggc acgtccgcgg
     3781 tgatcgtcat caccgagcgg tccccctgcg tctcgatgat ttcgatggtg ttcacccggt
     3841 tgcggctgcc gccgatgcgg atctcgctca ccatgccctg catgtcgggc tcggccggct
     3901 tcagcgtaag gcgccactgg cgctcgccgc cgtccagggc cacctggtag aagcggttca
     3961 gcgtcgcgag atcgcccgcg agcgtggagc ggatgctctc gacgaaagcc cagatcacgg
     4021 gctgatcctg gagcgcaaag gtgcggcgct gattacgatc cttgttctcg agaatcagcc
     4081 ggtcgccttc cagcaccagg ctttccc
//
LOCUS       DBNGSG010000226         4965 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3977265, whole genome shotgun sequence.
ACCESSION   DBNGSG010000226 DBNGSG010000000
VERSION     DBNGSG010000226.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4965)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3977265"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="AC158Q_06015"
     CDS             <1..193
                     /locus_tag="AC158Q_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339364.1"
                     /translation="AAAGATGLAYFIAHRFLQLQYAPDPAVWLLGVGGTALGVAGAGW
                     LVTRRVLAVPPLQVLRSIG"
     gene            310..1515
                     /locus_tag="AC158Q_06020"
     CDS             310..1515
                     /locus_tag="AC158Q_06020"
                     /inference="COORDINATES: protein motif:HMM:NF014680.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HIR3339365.1"
                     /translation="MPDILSVVLALAILAALVVLIVRLAGQAAAVRSLQEEMVRALEE
                     KHRAMLTDLHGGLAQQSDRLASRLSEELNQTRETLHRLQLSLGASLGDTTEKLNAKID
                     QRLEQISGKVNERLDEGFKKTNETFINVMQRLATIDEAQKKIETLTGSVVSLQELLGN
                     KQSRGAFGEVQLEALVRNVLPPAAYEMQYTLPDGARADCVLKLPEPTGLVCVDSKFPL
                     ENYHRMLAPGANDVERAGAQRQFRADIRKHVDDIAKKYIVPNITSDGAVMFVPAEAVF
                     AEIHAYHAEVVDYANLKRVWIVSPTTLMAVLNTARAVLKDVETRKQIHVIREALRRLA
                     VEFSRFDERMKELSRHIRQAAEDVDRIQVTGGKISQQFQRIESAELEPPEAAQLKVVE
                     MRDDGKGSK"
     gene            1577..2377
                     /locus_tag="AC158Q_06025"
     CDS             1577..2377
                     /locus_tag="AC158Q_06025"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HIR3339366.1"
                     /translation="MFEILKNWRRERVLRRSTLDDAQWHAALDRYPFTRVLSADERGR
                     LRERVILFLHEKSIVGAGGLVLHDEMRTGIAVQACMLILNLDLGYYRGWSEVIVYPDE
                     FVAEYEYMDEHGIVHRVEEPMSGESWLAGPVILSWADTQLRGDGHGYNVVIHEFAHKL
                     DMLNGEANGFPPLHAGMDRGEWSRVFGAAYERFNARIQRGELPGLDPYAGENPAEFFA
                     VISEAFFEVPETVLAAFADVYRQLAAFYRQDPAARLAHEAALERAAAR"
     gene            2374..4770
                     /locus_tag="AC158Q_06030"
     CDS             2374..4770
                     /locus_tag="AC158Q_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR3339367.1"
                     /translation="MMPSLSGTGSKSLLFLAFGLLLSVITALIALGLARVESFNRQVN
                     ALSEAQGRKIGTVSELFLANGQRSTLIDKLFAAETPQARRAVLEHYQRAIAGYSGAVK
                     RLVTLQVDATEREARDTAIAAAGTSRVMGDRIVALLMQGEISTASELNLTQAVQTDSR
                     LQETLYLLLEANHARTAQAIAAANDGMRHGFLLIGAGGLLALLAGIVTAVLVIRVIAR
                     TESRLEREKELAEVTLHSIVDGVITTDASGRVEYLNPVAERYLGWTSAEVAGRGLAEV
                     YRVVDERTGKSIESLPLTDMPATEGGEPIAVRLVDRYGRECPVRYSYSPIRGRDGTVH
                     GMIVVFHDVSQVRAMAQQLIWQASHDSLTGLVNRREFERRLAELVETARTQRREHALL
                     FMDLDNFKAVNDTCGHSAGDELLRQLTAIMLTRMRGSDTLARLGGDEFGALLESCPLE
                     QAVRIANAMRETVREFRFVWEGKTFSVGVSIGLVPIGAESGDLNQLLAAADACCYEAK
                     NQGRDRVQVYRPDSPGYGGRHEELQLVSQINRAFELGHFRLYRQRIAPLGGASQHEPH
                     YEVLIRMLDRDGNAVPATGFMPAAERYNLLTSIERWVVSSLVEFLHRQWTTGAISHAP
                     HGGGERGFYSVNISGASINDRSFPDFLRNLLTRYQLPSGLLCFEITETTAISNLSKAA
                     ELMHELKGMGCRFALDDFGTGMSSFAYLKYLPVDFLKIAGVFIKDIVGDPMDNAIVDS
                     INRISHILGMRTVAESVEDAAILARITELGLDYAQGYFVAEPEELGDEPAQRQTALFA
                     "
     gene            4784..>4965
                     /locus_tag="AC158Q_06035"
     CDS             4784..>4965
                     /locus_tag="AC158Q_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HIR3339368.1"
                     /translation="MKQGRLLVADVGGTWTTIALAPAGADPVIAFHHVYPSQNYDGLE
                     AIVTEFFARPEVAMHGG"
BASE COUNT          886 a         1559 c         1680 g          840 t
ORIGIN      
        1 ggcggctgcc ggggcaaccg gcctggcgta tttcatcgcg caccgtttcc tgcagctgca
       61 atatgcaccc gacccggcgg tgtggctgct cggggtcggc ggcaccgcgc tgggcgtggc
      121 cggggccggc tggctggtca cgcgccgcgt gctcgcggtg cctccgctcc aggtgctgcg
      181 ttcgatcggt tgatgccaat ggacaggaaa tagaaaacaa gaaaggagga aacaggagag
      241 ggcgcggggg cttctatcgt tccatctcct attcccaatt tcccatttcc ccttcatact
      301 ttgcaggcga tgcccgacat cctttctgtg gttctggcgc tcgccatcct cgccgcactc
      361 gtcgtgctga tcgtgaggct ggcaggccag gccgccgcgg tgagatcgct gcaggaagag
      421 atggtccggg cgctcgagga gaagcatcgc gcgatgctga ccgacctgca cggcggtctc
      481 gcgcagcagt ccgaccgcct ggcgtcacgc ctgtccgaag agctcaacca gacgcgtgag
      541 accctgcacc gcctccagtt gtcgctgggc gcgagcctgg gcgacaccac cgagaaattg
      601 aacgccaaga tcgatcagcg cctggagcag atctccggca aggtgaacga gcggctggac
      661 gagggcttca agaagaccaa cgagaccttt atcaacgtga tgcagcgcct ggccacgatc
      721 gacgaggcgc agaagaagat cgagacgctg accggcagcg tggtaagcct gcaggagctg
      781 ctgggcaaca agcagtcccg cggcgccttc ggcgaggtgc agctggaggc gctggtgcgc
      841 aacgtcctgc cgcccgcagc ctatgaaatg caatacacgc tgcccgatgg cgcgcgcgcg
      901 gactgcgtgc tgaagctgcc cgagccgacc gggctggtgt gcgtggactc caaattcccg
      961 ctcgagaact accaccggat gctcgctccg ggagcgaacg acgtggaacg cgcgggagcc
     1021 cagcgccagt tccgtgccga tatccgcaag cacgtggatg acatcgcgaa gaaatacatc
     1081 gtgccgaaca tcacctcgga cggcgcggtc atgttcgtgc cggcggaagc ggtgttcgcc
     1141 gaaatccacg cttatcatgc cgaggtcgtg gattacgcca atctgaagcg cgtatggatc
     1201 gtgtcgccca cgacgctgat ggcggtgctc aataccgcgc gcgccgtgct gaaggatgtc
     1261 gagacccgca agcagatcca tgtcatacgc gaggcgttga ggcgcctggc agtggaattc
     1321 agccgtttcg acgaacggat gaaggagctg tcgcgccaca tcaggcaggc ggcggaagac
     1381 gtggaccgga tacaggtcac gggcggaaag atttcccagc agttccagcg catcgagtcg
     1441 gcggagctgg agccgccgga ggcggcccag ctgaaggtcg tcgaaatgag ggacgatggc
     1501 aaggggagca agtgagcaag accccggcag ccgtcagcgc gagcgcacgt cttcaggcag
     1561 cgcggccccg gtaacgatgt tcgagatcct gaaaaactgg cgccgggagc gggttctcag
     1621 gcgctccact ctcgacgatg cccagtggca cgccgcgctc gaccgttatc cctttacccg
     1681 cgtgctgtcc gcggacgaac gcggccggtt gcgcgagcgg gtgatccttt ttttgcacga
     1741 gaaatccatc gtcggagccg gcggcctcgt gctgcacgac gaaatgcgca ccggcatcgc
     1801 ggtgcaggcc tgcatgctga tactcaacct cgatctcggc tattaccgcg ggtggagcga
     1861 ggtcatcgtg taccccgatg aattcgtcgc cgagtacgag tacatggacg agcacggcat
     1921 cgtgcaccgg gtggaggagc cgatgtccgg ggagtcctgg ctagcggggc cggtgatcct
     1981 gtcatgggct gatacccagt tgcgcggaga cgggcacggt tacaacgtgg tcatccacga
     2041 gtttgcccac aagctcgaca tgctgaacgg cgaagccaac gggtttccgc cgctgcatgc
     2101 gggaatggac cggggggagt ggtcccgggt cttcggggcg gcctatgagc gtttcaatgc
     2161 ccggatccag cgcggcgaat tgcccgggct cgatccttat gccggagaga atccggccga
     2221 gtttttcgca gtgatcagcg aggcgttttt cgaagtcccg gaaaccgtgc tcgccgcgtt
     2281 cgccgacgtc tatcgccagt tggcggcgtt ctatcgccag gatccggctg cgcgcctcgc
     2341 gcacgaggcc gccttggagc gggccgccgc ccgatgatgc cttcactctc cggcaccggg
     2401 tcgaaatccc tgctgttcct ggccttcggg ctgctgctct cggtcattac cgcgctgatt
     2461 gcgctggggc tcgcgcgcgt cgagtcattc aaccggcagg tcaatgcatt gagcgaagcg
     2521 caggggcgca agattggcac ggtctcggag ctctttcttg ccaacggaca gcgctcgacg
     2581 ctgatcgaca agctgttcgc ggcagaaacg ccgcaggccc ggcgggccgt gctggagcat
     2641 taccagcgcg ccatcgccgg ttattcgggc gccgtaaagc ggctggtcac gctgcaggtg
     2701 gacgccaccg aacgcgaagc gcgcgatacg gcgattgcgg cggcgggtac ctcccgggtc
     2761 atgggagacc ggatcgtcgc gctgctgatg cagggtgaga tctcgacagc atcggagctg
     2821 aacctgacgc aggcggtgca gaccgacagc cggctgcagg agacgctcta cctcctgctg
     2881 gaagccaatc acgcccggac cgcacaggcg atcgcggcgg ccaacgatgg catgcgccat
     2941 ggctttcttc tgatcggggc gggcggcctg ctggccctgc tggccggcat cgtgacggcg
     3001 gtcctggtta ttcgtgtcat tgcgcgcacc gagtcgaggc tcgagcggga gaaagagctt
     3061 gcggaagtca cgctgcattc catcgtggac ggggtgatca ccaccgacgc atccggccgc
     3121 gtcgagtacc tcaatccggt cgccgagcgt tacctgggct ggaccagcgc cgaggtggcc
     3181 ggcagggggc tggccgaagt ctatcgcgta gtcgacgagc gcaccggcaa atcgatcgag
     3241 tcgctgccgt tgaccgacat gccggccacc gagggcgggg aaccgatcgc ggtgcggctg
     3301 gtagaccgct acgggcgcga atgcccggtg cgctactcgt attctccgat ccgcgggcgc
     3361 gatggcactg tgcacggcat gatcgtcgtg ttccacgacg tgagccaggt tcgcgccatg
     3421 gcccagcaac tcatctggca ggcaagccac gactcgctga ccgggctggt caaccgccgc
     3481 gaattcgagc gccggctcgc ggagctggtc gagaccgcga gaacgcagcg gcgcgagcac
     3541 gcgctcctgt tcatggacct cgacaacttc aaggcggtga acgacacctg cgggcacagc
     3601 gcgggtgacg aattgctgcg acagctcacc gccatcatgc tgacccgcat gcgcggcagc
     3661 gacacgctcg cgcgcctggg cggagacgaa ttcggcgctc tgctggagtc gtgccccctc
     3721 gagcaggcgg tgcgcatcgc caacgccatg cgcgagacgg tgcgcgagtt tcgtttcgta
     3781 tgggagggca agactttcag cgtgggcgtg agcatcgggc tggtgccgat cggcgcggag
     3841 agcggggact tgaaccagtt gctcgcggcc gctgatgcct gctgctacga ggccaagaac
     3901 cagggcaggg atcgggtgca ggtctaccgg ccggacagcc ccggctacgg cggccggcac
     3961 gaggagctgc agctcgtgtc ccagatcaac cgcgcgttcg agctcggaca tttccggctc
     4021 taccgccagc gcatcgcgcc actcggcggc gcctcgcagc acgagccgca ttacgaagtg
     4081 ctgatacgca tgctggaccg tgacggcaac gcggtgccgg ccaccgggtt catgccggcg
     4141 gcggagcgct acaacctgct cacttcgatc gagcgctggg tggtgagttc gctggtggag
     4201 ttcctgcacc ggcagtggac gaccggcgcc atctcccacg cgccgcacgg cggcggagag
     4261 cgcggcttct actccgtcaa catctcgggc gccagcatca acgacaggag ttttcccgat
     4321 ttcctgcgta atctcctgac ccgctaccag ttgccgagcg ggctgctgtg cttcgagatc
     4381 acggaaacca ccgcgatttc gaacctgtcg aaggcggcgg agctgatgca cgagctgaag
     4441 gggatgggtt gccgcttcgc gctcgacgat ttcggcaccg gcatgtcgtc gtttgcctat
     4501 ctcaagtacc tgccggtgga ctttctcaag atcgccggcg tgttcatcaa ggacatcgtg
     4561 ggcgacccca tggacaacgc catcgtggac tcgatcaacc gcatcagcca tatcctgggc
     4621 atgcgcacgg tggccgaatc ggtggaagat gccgcgatac tggcgcggat caccgaactc
     4681 gggctcgact atgcccaggg atatttcgtc gcggaacccg aggaactcgg cgacgagccg
     4741 gcgcagcggc agaccgcgct gttcgcctga ggggacgccg ggcgtgaagc aggggcgact
     4801 gctcgtggcc gatgtcggcg gcacctggac aacgatcgcc cttgccccag ccggagccga
     4861 tccggttatc gccttccacc acgtttatcc gagccagaac tacgacggcc tcgaagccat
     4921 cgtcaccgag ttttttgccc ggccggaagt ggccatgcac ggcgg
//
LOCUS       DBNGSG010000227         6411 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2619785, whole genome shotgun sequence.
ACCESSION   DBNGSG010000227 DBNGSG010000000
VERSION     DBNGSG010000227.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6411)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6411)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6411
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2619785"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="AC158Q_06040"
     CDS             complement(<1..249)
                     /locus_tag="AC158Q_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339369.1"
                     /translation="MNSQAVIRELTARAFALLAAGFLLLMLGCATRPTGLTNEGRTHL
                     GEIAVAKGYEKPEVKFSGVPNAGTGAMKGILLGILNPMA"
     gene            complement(368..1687)
                     /locus_tag="AC158Q_06045"
     CDS             complement(368..1687)
                     /locus_tag="AC158Q_06045"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HIR3339370.1"
                     /translation="MPLYRFRILGAIAAACVLLAPVPAAANRIVGGWSAAGIAHLPAE
                     NVRLLNKNNEVLATVSRTRVVRLVDVLSRVEKSAGVTAELFVVQYGDSQPNAFAVGKE
                     GRNIVGITPAMLELVGDDFDAYAAIFGHELAHLVKEHGSGRASRQGLLQGLGLIAGIL
                     IGARTGINPSSLIDLGTTIADRSFSREEEREADRLGVEYMIAAGFDSQGAVRLQQRMS
                     EAGKAGSTGFLSTHPSGEERIANLQQILASQPPAAKPPAVARSSDRKDVEELVRRGNW
                     YGLLAVSEEQATANPTDAFWWFHKALAHQKLGDHKEAVPAYQNALKHEPEYKSALLNL
                     GYSYLQTKQPDKSAPAFEGAIRATPDNASAWFGLGIAYFQLGQRDKAWDTHQELKKLD
                     PKRAERFAQIASVPAERPEPQPPNASGVSETMPGQAQDETQEPEAHP"
     gene            complement(1699..2787)
                     /locus_tag="AC158Q_06050"
     CDS             complement(1699..2787)
                     /locus_tag="AC158Q_06050"
                     /inference="COORDINATES: protein motif:HMM:NF021504.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HIR3339371.1"
                     /translation="MWSTHQFWVGLLEALGVNPRKIVFSSDTSEEQGRAFAKGRGTVD
                     CCYPVKSISGHYGELLARDKHRKIDVLFSPMIYSLPSFMKGHVLDTLACPRVMAAPEN
                     IKAGFLKEKDAFAERNIKYVAPLASLGDPPLVPKQLYPALKEIFPELTLEETKEAVRK
                     GFEALEAINTQARKRGRSILEQCARENKPCLLVIARPYHMDPGIGHEIEVDLQAYGYP
                     VLWTQYFPTDPDLLEWMFAEDLKSGLIRSPFDISDVWPSSYSSNTNETLWGAKVAARM
                     PWIAGVIRLASYECGMDQPTYSPVQSIVERSGTLFFSFQELDSTKPAGSVKIRVETIA
                     HYLQKYAGEIIAKKKAVSPGNCPLVRLH"
     gene            complement(2750..4981)
                     /locus_tag="AC158Q_06055"
     CDS             complement(2750..4981)
                     /locus_tag="AC158Q_06055"
                     /inference="COORDINATES: protein motif:HMM:NF013986.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HIR3339372.1"
                     /translation="MKIVGMDVGSTTVKAVLMEDGRQLWRDYKRHNTKQAEMVFEFLG
                     RMESECGLAPKEARMFFTGSGAGFLAPLVGAKVIQEVVAVSAAVEKLHPGIRFVSEIG
                     GEDMKTVFFTPSGDGTSKQVFMQSACSGGTGTFIEKTARKLSIETESLAKMPYEGKSL
                     HKVSSKCGIFAEADSNTLVKSGVPVEEIIASLYEAVVYQNLATLTKGNTPAPEVLLLG
                     GPNLFFEGLQQAWRFHLKKLWKERKIALPEGKTAEEVIHVPADALYYACLGCIEIGLG
                     ENPEVALYQGHDKLRWWIDQGQHEQKAKDGARGLIAGEDDFKAFLSKYDQPKTAAIAT
                     ACSTSDAVFLGCDFGSTTAKAVVLSREKEQLFSCYVQSKGNPIEDAKAILRQISEAGY
                     RNIGGLAITGYGKDLLKDIIGADVGLVETVAHATAALHFFPDADVICDVGGCDVKIML
                     LRLGTVSDFRLNSQCSSGNGAFLQGVAERYAIPLEQYADNAFKARSIPTLAMGCGVFL
                     QSDIVNQQRKGWSAEEIMASLAAVLPLNVWVYAGQLQNLAAAGRKFVLQGGTHRNLAV
                     VKAQVDFITSKVPNAEIVVHPFPGEAGAIGAAICAMERAEGGKPSRFRGFDTIEALEY
                     TSTTNEHTTCRWCPVSCRRTFIDVVLPGAKGRPWSKVPLGPGWERVISGNSCPKGLLE
                     DVNEMKVVKAQMEEVKRAYPNVAEMVREDAFRHARVPGEAEDRDSEGAEHVVNPSVLG
                     RPA"
     gene            complement(4978..>6411)
                     /locus_tag="AC158Q_06060"
     CDS             complement(4978..>6411)
                     /locus_tag="AC158Q_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339373.1"
                     /translation="LFGGLHWRAERLIQGAMENLGYRSRVLPTATREDLLTGRELADI
                     GQCCPTSFTTGNLANFLRAQSRESSPEEVAKKYVYVTAGACGACRFGQYHQSYELALR
                     NVGMESFRMFLLAQNGLDQGAAEGGGLELNLPFTMGAVWAVVVGDLVQDLEYQTRPYE
                     VRPGETERVVRESIEFLHGKFLKSPVRGKKWSTPLWYLTSDYFVEALREVHRRFEAIE
                     LDRLRVKPVVKITGEFYLQTVEGAPNYNIHQWLEQEGAEVYPAAITIWLDYLMRHAGQ
                     DYEDHIGIDRLARLKLGAVKTGMRLLRRTYDGMRSALGGLPHEMPDQYELRELASPYY
                     HSRLNGGEGDMLIGKALWAHHHKKAHMICELSPYSCMPNTMSIGAMAAVIGKHPDLLY
                     APLEIKGDAEVHALSRCQMILTEAKRRAQREFEDTLKVNGLTLERARELLESFPEAKR
                     ATYRIPRHGTAGTAASLVQHLADKARQ"
BASE COUNT         1042 a         2065 c         2018 g         1286 t
ORIGIN      
        1 tgccattggg ttgagtatcc caagcaagat acctttcatc gcacctgtac cggcgttcgg
       61 cacccccgag aatttcactt ccggtttttc gtatcctttc gcaactgcaa tttcccccag
      121 gtgtgtccgc ccctcattgg tcaaccccgt cgggcgcgtt gcgcaaccca gcatgagcaa
      181 caggaatcct gctgccagca atgcaaaagc acgagctgtc aattctctga tgacggcctg
      241 cgaattcatc gtttcacagc ggcaagaacg cagtcgccgc taatcatcta gcgttggggt
      301 acgggtatat ggcgcggtac tgaagccata tccgtttggc caggtattgg cagtacaggg
      361 gctgtgctca tggatgtgcc tcgggctcct gggtttcatc ctgcgcttgg cccggcatgg
      421 tttcgctcac gccggatgcg ttcggtggct gcggctcagg tctttccgcg ggaacggagg
      481 cgatctgggc gaagcgttcg gcccgcttgg ggtcgagctt cttcagttcc tggtgcgtat
      541 cccacgcctt gtcgcgctgc cccagctgga aataggcgat acccaggcca aaccaggcag
      601 atgcattgtc gggtgttgca cggatggcgc cctcgaaagc gggggccgac ttgtcgggct
      661 gcttcgtctg caggtaactg tagccgagat tgagcaacgc gctcttgtat tcgggctcat
      721 gcttgagcgc gttctgatag gccggcacgg cttccttgtg atcccccagc ttctggtgtg
      781 cgagtgcctt gtgaaaccac cagaacgcgt ctgtcggatt ggccgtggct tgctcttcgg
      841 acaccgccag aagtccgtac cagtttccgc gccgaacaag ctcttcgaca tctttcctgt
      901 ccgagcttct cgcaacggca ggaggcttgg ccgcaggcgg ttgagacgcc aggatctgct
      961 gcagattggc gattctctct tccccgcttg gatgcgtgct caggaacccg gtagagcctg
     1021 ctttcccggc ctccgacatc cgctgctgca accgaacggc tccctgcgaa tcgaagcccg
     1081 ccgcgatcat gtactcgacg cccagtcgat ccgcctctct ttcttcctcg cgcgaaaacg
     1141 atcgatcggc tatggtggtc ccgagatcga tcaagctgga cggattgatt cccgtccgcg
     1201 cgccgatcag gatgcctgcg atcaacccca gcccctgaag caagccttgc cgcgacgcgc
     1261 ggcccgagcc gtgctccttc acgagatgcg ccagctcgtg tccgaatatc gcggcataag
     1321 catcgaaatc gtcacccacc agctccagca tcgcaggcgt tatgccgaca atgttccgcc
     1381 cctccttgcc gacggcaaat gcgttgggct gactgtcccc atattgcacc acgaacagtt
     1441 ccgcggtgac gccggcagat ttctccacgc gggacagcac gtcaaccagc cggacaactc
     1501 tcgtcctgct cacggtggcc aagacttcat tgttcttgtt caggagcctg acattttcgg
     1561 ccggcaggtg tgcaattccc gccgctgacc aacccccgac gatgcggttg gccgcggccg
     1621 gaacgggcgc aagcaatacg cacgctgcgg caattgcgcc aagaatcctg aatctgtaga
     1681 ggggcatcgt ggtgacggtc aatgcaagcg cacgagcggg cagttgccag ggctcacggc
     1741 ctttttcttc gcgatgatct cgcccgcgta cttctgcagg tagtgcgcga tggtctcgac
     1801 gcggatcttc accgagccgg cgggcttggt ggagtcgagc tcctggaacg agaagaagag
     1861 ggtgccggag cgctcgacga tactctgcac cgggctgtag gtcggctggt ccatgccgca
     1921 ttcgtagctc gcgaggcgga tcacgcccgc gatccatggc atgcgcgcgg cgactttcgc
     1981 gccccatagc gtctcgttgg tattggagct gtaggacgac ggccacacgt cggagatgtc
     2041 gaacggcgag cggatgaggc cggacttcag gtcttcggcg aacatccact cgagcaggtc
     2101 gggatcggtc gggaagtact gcgtccacag caccggatag ccgtaggcct gcaggtccac
     2161 ttcgatctca tggccgatgc cggggtccat gtgatagggc cgcgcgatga cgagcaggca
     2221 cggcttgttc tcccgggcac actgctcgag gatggaacgg ccgcgcttgc gtgcctgggt
     2281 gttgatggcc tcgagcgcct cgaacccctt gcgcactgct tccttcgttt cctcgagcgt
     2341 gagctcggga aaaatctcct tgagcgcggg gtagagctgc ttcggaacga gcggcggatc
     2401 gccgagcgag gcgagcggcg cgacgtactt gatgttgcgc tccgcgaatg cgtccttctc
     2461 cttgagaaag cccgccttga tgttctccgg cgcggccatt acgcgcgggc acgccagcgt
     2521 gtcgagcacg tgccccttca tgaacgaggg cagcgagtag atcatcggcg agaacagcac
     2581 gtcgatcttg cggtgcttgt cgcgcgcgag gagctcgccg taatgcccgg agatgctctt
     2641 tacggggtag cagcagtcca ccgtgccgcg gcccttggcg aaggcgcggc cctgttcctc
     2701 cgacgtgtcg gaggagaaga cgatcttgcg cgggttgact ccgagcgcct caagcaggcc
     2761 gacccagaac tgatgggttg accacatgtt cagcaccctc ggaatcccga tcctcagctt
     2821 cgcccggtac tcgggcgtgg cggaaggcgt cctcgcggac catttctgca acattgggat
     2881 aagcacgttt gacctcctcc atctgggctt tcacgacttt catttcgttg acgtcctcga
     2941 gcagcccctt ggggcacgag ttgccgctga tcacccgctc ccagccgggg ccgagcggga
     3001 ccttgctcca tggcctgccc ttggcgccgg gcagcacgac gtcgatgaac gtgcgccggc
     3061 acgacaccgg gcaccagcgg caggtggtgt gctcgttggt ggtgctggtg tattcgaggg
     3121 cctcaatggt gtcgaagccg cgaaaccggc ttggcttgcc gccttccgca cgctccatgg
     3181 cgcagatcgc cgcaccgatc gcgccggcct cgcccggaaa cgggtggacc acgatctccg
     3241 cgttcggcac cttgctggtg atgaagtcca cctgcgcctt taccaccgcc aggttacggt
     3301 gcgtgccccc ctgcaggacg aacttcctgc cggcggccgc caggttctgc agctggcccg
     3361 catagaccca gacgttgagc ggaaggaccg cggcaagcga ggccatgatt tcctccgccg
     3421 accagccctt gcgctgctgg ttcacgatgt cggattggag gaacacgccg cagcccatcg
     3481 cgagcgtcgg gatcgagcgc gccttgaacg cgttgtcggc gtactgctcg agtggaatcg
     3541 cgtagcgctc ggccactccc tgcaggaacg cgccgttgcc cgaggagcac tgcgaattca
     3601 gccggaaatc cgacaccgtg cccaggcgca gcagcatgat cttcacgtcg catccgccga
     3661 cgtcgcagat gacgtcggcg tcggggaaga agtgcagtgc cgcggtggcg tgcgcgacgg
     3721 tctccaccag ccccacgtcg gcgccgatga tgtccttcag gaggtccttg ccgtagccgg
     3781 tgatcgcaag gccgccgatg ttccggtagc ccgcctcgct tatctggcgc aggatcgcct
     3841 tggcgtcctc gatcgggttg cctttcgact gcacgtagca ggagaacaac tgctcttttt
     3901 cgcgcgatag cacgacggcc tttgccgtgg tgctgccgaa gtcgcagccg aggaacaccg
     3961 cgtcagacgt ggaacacgct gttgcgattg cggccgtttt cggctggtcg tacttcgaaa
     4021 gaaatgcctt gaagtcgtcc tcacccgcga tcagcccgcg cgcgccgtcc ttcgctttct
     4081 gctcgtgctg gccctggtct atccaccagc gcagcttgtc gtgcccctgg tagagcgcga
     4141 cttccgggtt ctcgccgaga ccgatctcga tgcagcccag gcaggcgtag tagagcgcgt
     4201 ccgctggcac gtggatgacc tcttccgcgg tcttgccctc gggcagcgcg attttccgct
     4261 ccttccacaa cttcttcagg tggaagcgcc aggcctgctg cagtccctcg aagaacaggt
     4321 tgggcccgcc cagcagcagc acctcgggcg ccggcgtgtt gcccttggtg agggtcgcga
     4381 ggttctggta gaccactgct tcgtagaggc tagcgatgat ttcctccacc ggcacgcccg
     4441 acttcaccag ggtgttggag tcggcctcgg cgaagatccc gcacttggag ctgactttgt
     4501 gcaggctctt gccctcgtac ggcatcttgg cgaggctctc ggtctcgatc gagagcttgc
     4561 gcgcggtctt ctcgatgaac gtgccggtgc cgccgctgca ggcggactgc atgaacacct
     4621 gcttgctggt gccatcgccg ctcggggtga aaaacacggt cttcatgtcc tcgccgccga
     4681 tctccgagac gaagcggatg ccggggtgga gtttttccac cgccgcggaa acggcgacca
     4741 cctcctggat caccttggcg cccacgagcg gcgcgagaaa gccggcgccg gagccggtga
     4801 agaacatccg ggcttccttt ggcgcgaggc cgcactcgga ttccatgcgc ccgagaaatt
     4861 cgaacaccat ctcggcctgc ttggtgttgt ggcgcttgta gtcgcgccac aactgccgcc
     4921 cgtcctccat cagcaccgcc ttgacggtgg tggagccgac gtccatgccg actattttca
     4981 ttggcgtgcc ttgtccgcga ggtgctgcac gaggctcgca gcggtgcctg ccgtaccgtg
     5041 acgcgggatc ctgtaagtcg cgcgctttgc ctccgggaag gattcgagca attcacgcgc
     5101 ccgctcgagc gtgaggccgt tcactttcag tgtgtcctcg aattcgcgct gcgcgcggcg
     5161 cttcgcctcg gtgaggatca tctggcaacg cgacagcgcg tggacttccg cgtcgccctt
     5221 gatctcgagc ggcgcgtaga gaaggtcggg atgcttgccg atcaccgccg ccattgcccc
     5281 gatgctcatg gtgttgggca tgcaggagta cggtgagagc tcgcagatca tgtgcgcttt
     5341 cttgtggtgg tgggcccaca gcgccttgcc gatcagcatg tcgccttcgc cgccgttgag
     5401 gcggctgtgg taataagggg aggcgagctc gcgcaattcg tactggtccg gcatttcgtg
     5461 cggcagaccg cccagcgcgc tgcgcatgcc gtcgtaggtc cggcgcagca accgcatgcc
     5521 ggtcttcacc gcgcccagct tcagtcgcgc gagccggtcg atgccgatgt gatcctcgta
     5581 gtcctggccg gcatggcgca tcaggtagtc gagccagatg gtgatggcgg cgggatagac
     5641 ttccgcgcct tcctgctcga gccactggtg aatgttgtag ttgggcgcac cctcgaccgt
     5701 ctgaagatag aactcgccgg tgatcttcac cacgggcttc acccgcaggc ggtccaactc
     5761 aatggcctcg aaacggcgat gcacctcgcg cagcgcttcc acgaagtagt cgctggtcag
     5821 gtaccacagt ggcgtgctcc atttcttccc gcgcacgggc gacttgagga atttcccgtg
     5881 caggaactcg atgctttccc tgacgacgcg ctcggtctcg ccgggccgga cttcgtacgg
     5941 gcgcgtctgg tactcgaggt cctgcaccag gtccccgacc actaccgccc acaccgcgcc
     6001 catggtgaaa ggcagattga gttccagccc cccgccttcc gccgcgccct ggtctaggcc
     6061 gttctgcgcg agcaggaaca tgcggaacga ttccatgccg acgttgcgca gcgcgagctc
     6121 gtagctctgg tggtactgcc cgaagcggca ggcgccgcag gcgccggcgg tgacgtacac
     6181 gtacttcttg gcgacttcct cgggactcga ttcgcgcgac tgcgcgcgca ggaagttcgc
     6241 caggttgccg gtggtgaagc tcgtcgggca gcactggccg atgtccgcca actcgcgccc
     6301 ggtgagcagg tcctccctgg tggcggtggg caggacccgg ctgcggtagc cgaggttttc
     6361 catcgcgccc tggatcagcc gctcggcgcg ccagtgcagg ccgccgaaga g
//
LOCUS       DBNGSG010000228         3745 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1092403, whole genome shotgun sequence.
ACCESSION   DBNGSG010000228 DBNGSG010000000
VERSION     DBNGSG010000228.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3745)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1092403"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="AC158Q_06065"
     CDS             complement(<1..350)
                     /locus_tag="AC158Q_06065"
                     /inference="COORDINATES: protein motif:HMM:NF052759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIR3339374.1"
                     /translation="MEALLLAIYSFFVWLIFIKLKWLPWNIKTQVIVVIIPIVGMAAL
                     ILLLNIFAPSSSDVRVFKYIVPIVSQVRGRVIEVPVEEGNRLVKKGEVLFKIDPEPYR
                     LAVNQLEAQLATAVA"
     gene            complement(350..895)
                     /locus_tag="AC158Q_06070"
     CDS             complement(350..895)
                     /locus_tag="AC158Q_06070"
                     /inference="COORDINATES: protein motif:HMM:NF023170.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HIR3339375.1"
                     /translation="MKPHESGTAGRVALLALLLAPRPAHAAFLPPELMAAAADWVALF
                     VLIVVPIGAVIVFWLVHILPEKIAEKRHHPQKSAINTLCLLSLAFGGLLWPIAWLWAY
                     SRPVAFRLAYGTDKHEDYFREMAREARAGKLTREEVVQLKGELDDIESRRILSPELKR
                     FRDEFAAVLAGAAQTDVRGAS"
     gene            complement(963..1319)
                     /locus_tag="AC158Q_06075"
     CDS             complement(963..1319)
                     /locus_tag="AC158Q_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339376.1"
                     /translation="MRLRINLRRWCFVMSAAVLLGGCVVYEPVPGYYVERSNFDRAWN
                     ASLGGVQDAGVRVISAEREAGLIRGARNGIDVVVTVARQPDGSVRVKFDAAGPVENDP
                     GLGERFSQAYERRMGR"
     gene            complement(1366..1914)
                     /locus_tag="AC158Q_06080"
     CDS             complement(1366..1914)
                     /locus_tag="AC158Q_06080"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3339377.1"
                     /translation="MRGVATEAEFLPVELELREGRRVTVRAVRPQDKEDVQAFLKRLS
                     EQSRYSRFMSTLRELSPKMLERAVNPEQGRELQLVAICGEGAGRTIVGGARYSAAAGS
                     KDCEFALTVDDAWQGFGLARRLLEALIGAARAAGFERMEGHILASNARMLGLAGRLGF
                     ERVESPEEPTVRLVRRDLSRPG"
     gene            complement(1929..2744)
                     /gene="ppk2"
                     /locus_tag="AC158Q_06085"
     CDS             complement(1929..2744)
                     /gene="ppk2"
                     /locus_tag="AC158Q_06085"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012404748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HIR3339378.1"
                     /translation="MASDETKKGKPDGSGRLKRKKYDKQLRKLHVELVKLQEWIRHKG
                     LKVCIVFEGRDGAGKGGTIKAITERVSPRVFRVIALPAPTEREKSQMYIQRYLPHLPA
                     AGEVVIWDRSWYNRAGVERVMGFASEETVKRFLRLAPPFEKIMVESGIILIKYWLEVS
                     MEEQTRRLKARIDDGRKIWKLSPMDLLSYSRWHDYSRARDDMFKATDTKWAPWHVVRS
                     DDKRRARLNVITHLLSRIPYKKAPREKVKLPKRGKAHGYKETNYPFKFIPERF"
     gene            complement(2767..3012)
                     /locus_tag="AC158Q_06090"
     CDS             complement(2767..3012)
                     /locus_tag="AC158Q_06090"
                     /inference="COORDINATES: protein motif:HMM:NF012305.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0051781 - positive regulation of cell
                     division [Evidence IEA];
                     GO_process: GO:0051984 - positive regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA recognition motif domain-containing protein"
                     /protein_id="HIR3339379.1"
                     /translation="MKLWIGNIEPGTTDDEIREFVKKYSPDLEIKAIQRVDGDGSRPA
                     ASIEFVDTPYGSVEKISMRLHGMYWKGRELFVQTVTR"
     gene            3233..>3745
                     /locus_tag="AC158Q_06095"
     CDS             3233..>3745
                     /locus_tag="AC158Q_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339380.1"
                     /translation="MMTSISPAVHGITSVRWQRVAGLAALLAAIMGPPTPVFGALTTG
                     DAYSYQLVNGYNKEVRGRVHYQVDRVDAEGVTVSVNPDNAEAGWAHTETYSKEGTWLR
                     NLVENHGVPVEYVFATAYPAYVFPLEPGKSWSVRVKATVPSLGARRSVRVDAWVIGAE
                     RIRVPAGEFDT"
BASE COUNT          663 a         1276 c         1130 g          676 t
ORIGIN      
        1 gccacggcgg tggcgagctg cgcctcgagc tggttgacgg ccaggcgata gggctccggg
       61 tcgatcttga acagcacctc gcctttcttc accagccggt tgccttcctc gaccggcacc
      121 tcgatcacgc gcccgcgcac ttgtgacacg atcggcacga tgtacttgaa cacgcgcacg
      181 tcggaggagg acggcgcgaa gatgttgagc agcaggatca gcgccgccat cccgacgatc
      241 gggatgatga cgacgatgac ctgcgtcttg atgttccacg gcagccactt cagcttgatg
      301 aagatcagcc agacgaagaa cgagtagatc gccagtagca gggcttccac taggacgccc
      361 ccctcacgtc cgtctgcgcc gcgccggcca gcaccgcggc gaactcgtca cggaaccgct
      421 tcagttccgg cgacaggatg cgccgcgact cgatgtcgtc gagctctccc ttcagctgca
      481 ccacttcctc gcgcgtgagc ttgccggccc gtgcctcgcg ggccatttcc cggaagtagt
      541 cctcgtgctt gtccgtgccg taggccaacc ggaacgccac cgggcgcgag tacgcccaca
      601 gccaggcgat gggccacagt agtccgccga acgccagcga cagcaggcat agcgtgttga
      661 tggcgctctt ctgcgggtga tggcgcttct cggcgatctt ttcgggcagg atatggacca
      721 gccagaacac gatcacggcg ccgatcggca cgacgatcag gacgaacagc gcgacccagt
      781 cggcggcggc ggccatcaac tccggcggca gaaacgccgc gtgtgcgggc cggggggcaa
      841 gcaagagagc aagtagcgcg acgcggcccg cagtccccga ctcatgcggc ttcatggcgt
      901 ctctccgaaa catgccttgt cactccattc ccctgccatg agcggcgcgt tgcgccgcag
      961 tgtcaccggc ccatccggcg ctcgtacgcc tgggagaacc tctcgccgag cccggggtcg
     1021 ttctcgaccg gccccgcggc gtcaaacttg acgcgtacgc tgccgtccgg ctgccgcgcg
     1081 acggtcacga ccacgtcgat tccgttcctg gcgccgcgga tgagccccgc ttcacgctcc
     1141 gccgagatga cgcggacgcc cgcatcctga acgcctccca acgacgcgtt ccatgcccgg
     1201 tcgaaattcg acctctccac ataatacccc ggcaccggct cgtagaccac gcaaccgccc
     1261 agcaacacgg cggcggacat gacgaaacac cacctgcgca gatttatcct caacctcatg
     1321 gcggctatcc tcccgctggc aatatggccc gcagcgacta ttcactcagc ccggcctcga
     1381 caggtcgcgc cggaccaggc gcaccgtcgg ctcttccggg ctctccacgc gctcgaaccc
     1441 cagtcgcccg gcaaggccca gcatgcgcgc gttggacgcg aggatgtggc cctccatgcg
     1501 ctcgaagccg gccgctcggg cggcgccgat cagcgcctcg agaaggcggc gcgcgaggcc
     1561 gaacccctgc caggcgtcgt ccacggtgag cgcaaattcg caatccttgc tgcccgcggc
     1621 ggcgctgtag cgtgcgccgc cgacgatcgt ccgccccgcc ccttctccgc agatggcgac
     1681 gagctgcagc tcgcggcctt gctcggggtt caccgcacgc tcgagcatct tcggagacag
     1741 ctcacgcagc gtggacatga agcgcgagta gcgcgactgt tccgaaagcc gcttcaggaa
     1801 agcctgcaca tcttccttgt cctgcgggcg cacggcgcgc acggtcaccc gccgcccttc
     1861 ccgcaactcc agctcgacgg gaagaaattc agcttcggtc gcgacgcccc tcaccggaca
     1921 gccgcccttc aaaaacgctc gggaatgaac ttgaacgggt aattcgtttc cttgtagccg
     1981 tgcgctttcc cccgtttcgg cagtttcact ttctcgcgcg gcgccttctt gtacggaatc
     2041 cggctgagca gatgggtgat gacattgagg cgcgcgcgcc gcttgtcgtc cgagcgcacc
     2101 acgtgccatg gcgcccattt cgtatcggtc gccttgaaca tgtcgtcgcg cgcccgcgaa
     2161 taatcgtgcc agcggctgta cgacaggaga tccatgggtg agagcttcca gatcttgcgc
     2221 ccgtcgtcga tgcgcgcctt gagccggcgg gtctgctctt ccatgctgac ctccagccag
     2281 tacttgatga ggatgatgcc ggactcaacc atgatcttct cgaaaggcgg cgccaggcgg
     2341 agaaatcgct tcaccgtttc ctccgaggca aaacccatca cgcgctcgac gcccgcgcgg
     2401 ttgtaccagc tgcggtccca gatcaccact tcaccggcag ccggcagatg cggcagatag
     2461 cgctggatgt acatctggct cttctcgcgc tccgtcggcg cgggcagcgc aatgacacga
     2521 aacacccgcg gactcacgcg ctcggtgatg gccttgatgg tgccgccctt gcccgccccg
     2581 tcgcgccctt cgaaaacgat gcagaccttg aggcccttgt gcctgatcca ttcctggagc
     2641 ttgaccagct cgacgtgaag ttttctgagc tgcttgtcgt acttctttct cttcagcctg
     2701 cccgagccgt cgggcttgcc ctttttcgtt tcgtcgctcg ccatgtgccc tcccgaccac
     2761 gcgccttcag cgcgtcaccg tctggacgaa tagctcgcgc cctttccagt acataccgtg
     2821 caggcgcatc gatatctttt cgaccgagcc gtagggcgta tcgacaaact cgatggacgc
     2881 cgccgggcgc gagccgtcgc cgtcgacgcg ctggatggcc ttgatctcga gatcggggga
     2941 atatttcttc acgaactcgc gaatctcgtc atcggtcgtg ccgggttcga tgttgcctat
     3001 ccagagcttc atgtcggcct cgtgtctggc atgcaaataa agtgtaaggg cgggggcgat
     3061 gtccgggctt gatgcaaatc aaggtcaacc ggagctcctg ttcatggtat cagaccaggt
     3121 ccgaaccggg ccccatcccg gaaagaaaaa aggaaaacca attgacgatg ccccgaatca
     3181 gcgcgcattc ttatggacaa gcgggctgta cccttttttg gagcgcccaa tcatgatgac
     3241 aagtatctct ccagcggttc atggcatcac ctccgtccgg tggcagcggg tagcgggcct
     3301 tgcggcgctc cttgccgcga taatggggcc gccaaccccc gtgttcggtg cactcaccac
     3361 gggtgacgcc tacagctatc aactcgtgaa cggctacaac aaggaggtcc gcgggcgggt
     3421 gcattaccag gtcgaccggg tggacgcgga gggcgtcacc gtttcggtca atccggacaa
     3481 tgccgaagcg ggatgggcgc acaccgaaac ctactcgaag gaaggcacct ggctgcgcaa
     3541 cctggtggaa aaccatggcg tgccggtgga gtacgtgttt gccacggcct accccgccta
     3601 cgtgtttccg ctggaaccgg ggaaatcctg gtcggtgcgc gtcaaggcaa ccgtgccgag
     3661 cctgggcgcg cgcagaagcg tgcgcgtgga tgcctgggtg atcggagccg agcgcatccg
     3721 cgtgccggcg ggtgaattcg acacc
//
LOCUS       DBNGSG010000229         3291 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1432011, whole genome shotgun sequence.
ACCESSION   DBNGSG010000229 DBNGSG010000000
VERSION     DBNGSG010000229.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1432011"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1908)
                     /locus_tag="AC158Q_06100"
     CDS             complement(<1..1908)
                     /locus_tag="AC158Q_06100"
                     /inference="COORDINATES: protein motif:HMM:NF019410.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HIR3339381.1"
                     /translation="MSNSDNPAATQQTTPFDSILAQSRELVCERLSDALTAMLDKVEE
                     RLTTLVSETQNRETQELYTETRDKTLAQRDAFEQQFRTRFLKEFQQRSNRVKKIGQSF
                     SEFDLSSLELDLVGEDDLEETLKFNNVAAKLRRFCDDELNALDQRVGVLLGDADLQSE
                     DNPFSPQAICDAYKHACRQVDTGVKVRMVLLKLFDDHVVDDIRSIYKDVNTLLVKNSI
                     LPKIRYGVSRQEGGKAPAAGARDPSEAPAAPAADKAPAGEQDFFSLMQNLMKSGGAGG
                     LSGFPAVPGAPGAVGADGAPIVVLQGAELLSSLTRLQRGDAGGTAEGGLPVIAPPGDA
                     GTTNVLRELKNSSVGAGMGQMDAMTLDIISMLFDQLFDEPRIPIGVKGLIGRMQIPML
                     KVAIADKAFFSKKTHPARQLLDTLGEMAVRLPADFNSSNPLFGRLEAIIQEVLVGFQD
                     NVEIFDAAREKVRALLAEDDQRLAKETEAAAKRIAQQESLAVAKVSAQDEIRARVRPG
                     KTWRPAVAFLVQHWVKFLLLVHVKRGKDSDVWKTAVETMDHLLWTVEPKKTLDERNKL
                     ASMVPGLVKRIGAGLKAAGVEDKVATRFFEELIKRHTEVIAPVVKAAEAAAAGNASKP
                     GPAPAGTAPAAP"
     gene            complement(2309..2494)
                     /locus_tag="AC158Q_06105"
     CDS             complement(2309..2494)
                     /locus_tag="AC158Q_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339382.1"
                     /translation="MARTPVKPAARNPRSNEARRSSPPASKKARQPADADRKQDADDI
                     RRAVDDGMQDLRMKKPG"
     gene            2732..>3291
                     /locus_tag="AC158Q_06110"
     CDS             2732..>3291
                     /locus_tag="AC158Q_06110"
                     /inference="COORDINATES: protein motif:HMM:NF012976.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase family protein"
                     /protein_id="HIR3339383.1"
                     /translation="MKNDDHQTGRILTRREALALLGGAAMLAGGVRPGVAQTLPSCVV
                     TPEQTEGPFFTDVRLNRSDIRTDPSDKSVKEGVALALNLRVSSVGAAGCVPLAGAMVD
                     VRHCDAEGVYTDSDRSTAGRKFLRGHQLTDADGAARFTTLYPGWYPGRAVHIHFKVRG
                     IAGPALGHEFTSQLYFDEAVNDRVLAR"
BASE COUNT          537 a         1064 c         1041 g          649 t
ORIGIN      
        1 tggcgcagcc ggcgcggtcc cggcgggcgc gggaccgggc ttgctcgcgt tgccggctgc
       61 cgcggcttcg gcggccttga cgaccggcgc gatcacctcg gtatggcgct tgatcagttc
      121 ctcgaaaaac cgcgtggcga ctttgtcttc gactccagcg gccttcaagc cggccccaat
      181 gcgcttgacc agcccgggca ccatcgaggc gagcttgttg cgttcgtcca gtgttttctt
      241 gggctcgacg gtccagagga gatgatccat ggtttcgacc gccgtcttcc acacgtcgct
      301 gtccttgccg cgcttgacgt ggacgagcag gagaaacttg acccagtgct gcaccaggaa
      361 cgcgaccgcc ggccgccacg ttttgccggg gcgcacccgg gcccggattt cgtcctgtgc
      421 cgagaccttc gccacggcga gagattcctg ctgcgcgatc cgcttcgcgg cggcctccgt
      481 ctccttcgcc agacgctggt cgtcttcggc gagcagcgcc cgcaccttct cgcgcgccgc
      541 gtcgaagatc tccacgttgt cctggaagcc gaccagcacc tcctggatga tcgcctcgag
      601 acgcccgaac agcggattgg aggaattgaa atcggcgggc aggcgcaccg ccatctcgcc
      661 cagcgtgtcg agcagctgcc gcgcgggatg ggtctttttc gaaaagaacg ccttgtcggc
      721 gatagcgacc ttcagcatcg ggatctgcat gcggccgatg agccccttca ccccgatggg
      781 aatcctgggc tcgtcgaaca actggtcgaa cagcatggaa atgatgtcca gcgtcatcgc
      841 atccatctgg cccatgccgg cgccgacgct gctgttcttc agctcgcgca gcacgttcgt
      901 cgttccggca tcgcccggag gcgcgatcac gggcaggccg ccctccgccg tgccgccagc
      961 gtcgccgcgc tgcaggcgcg tcagcgagct caacagctcc gcgccctgaa gcaccacgat
     1021 cggggcgccg tccgctccga ccgcgcccgg cgcgcccgga accgccggga aaccggacag
     1081 gccgcccgcc ccgcccgact tcatcaggtt ttgcatcaag gagaagaaat cctgttctcc
     1141 ggccggtgcc ttgtcggccg caggcgccgc gggcgcttcg gacggatccc gcgcgccggc
     1201 ggcaggcgcc ttgccgccct cctggcggga cacgccgtag cggatcttcg gcaggatcga
     1261 attctttacc agcaacgtgt tcacatcctt gtagatggac cggatgtcat ccacgacgtg
     1321 gtcgtcgaac agcttgagga ggaccatccg cactttgacg ccggtatcga cctgccggca
     1381 ggcgtgcttg taggcatcgc aaatggcctg ggggctgaag ggattgtctt ccgactgcag
     1441 gtcggcatcg cccagcagca cgcccacccg ctggtcgagg gcattgagtt cgtcgtcgca
     1501 gaagcggcgc aacttggccg ccacgttgtt gaatttgagg gtttcttcca gatcgtcttc
     1561 gcccaccagg tcgagttcga gtgaagacaa gtcgaattcg gaaaagctct ggccgatttt
     1621 cttgacccgg ttgctgcgct gctggaactc cttcaggaag cgggtccgga actgttgctc
     1681 gaacgcgtcg cgctgcgcga gcgtcttgtc gcgcgtctcg gtgtagagtt cctgtgtttc
     1741 ccggttctgg gtctcgctga cgagggtcgt tagcctttcc tcgaccttgt cgagcatggc
     1801 ggtcagagcg tcgctcagcc gctcgcagac cagttcgcga ctttgcgcca gaatgctgtc
     1861 gaacggcgtc gtttgctgcg tagcagcggg attatccgaa ttggacatgt gaacggatca
     1921 gcaccttgat caccccgctc cttgcatcgg gacgcgcagc cggtttggac tagaagcgcc
     1981 gcatcccccg atgggtgtgc atgaccctcg cacaacaccc ggttcgtgcg caaagcaacc
     2041 gccgcttaag cctattatgt ctcagtattt tccagacaaa acatgaccta ataccctaat
     2101 gataatggaa agctctcgcc ccacgctacg ttgtgtcagg tgggccttcg cgggccaggg
     2161 actcacgtaa ccttgacgta aggccattac aggaccggca ttattggcag aattaatggt
     2221 gcagcatcac ttccgatgag acgatgctgc gatgagggga ggaaccgacg aattggatga
     2281 actttcgcct tcgccttcag aacgtccgct acccgggctt tttcatcctc aagtcctgca
     2341 tgccgtcatc gacggcccgc cggatatcgt ccgcgtcctg cttgcggtcg gcgtctgccg
     2401 gttgcctggc ttttttcgac gcgggcgggc tgctgcggcg ggcttcgttt gaccgcgggt
     2461 tgcgcgcggc gggcttgacg ggtgttctgg ccatactacc ctcccctggg caagtgggca
     2521 gggagattct acaaggaacc cctgaaatca gggatgaagg cggagaatga gggatatgga
     2581 ggcaaggcgg gcaaaacaag ggtgccccgg gggcatttct ttcggcgaat ggaggtcgat
     2641 gtaacttttc catcgttccg gggtctgatt tcgcagcttc gggcacctgt tttccccgtc
     2701 gacacccccg tcactcgtca cctgtcactc tatgaagaac gacgaccatc aaaccggccg
     2761 catcctgact cgtcgcgagg cattggccct gcttggcggt gcggcgatgc tggccggcgg
     2821 cgtgcggccc ggcgtcgctc aaacgctgcc gtcctgcgtc gtgacgccgg agcagacgga
     2881 ggggccgttc ttcacggatg tgcgcctcaa tcgctccgat atccggaccg atccgtcgga
     2941 caagtccgtg aaggaaggcg tggcgctggc gcttaacctg cgcgtatcct ccgtcggcgc
     3001 cgcgggctgc gtcccgctcg cgggcgcgat ggtggacgtg aggcattgcg acgcggaggg
     3061 cgtttatact gattcggacc ggagcacggc cggccggaaa ttcctgcgcg gccaccagtt
     3121 gacggacgcg gacggcgccg cgcgcttcac gaccctctac cccggctggt acccgggccg
     3181 ggccgtgcac atccatttca aggtccgcgg gatcgccgga ccggccctgg ggcacgagtt
     3241 cacgtcgcag ctctacttcg acgaggcggt caacgatcgc gtcctggcgc g
//
LOCUS       DBNGSG010000230         3657 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2621076, whole genome shotgun sequence.
ACCESSION   DBNGSG010000230 DBNGSG010000000
VERSION     DBNGSG010000230.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2621076"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            303..1250
                     /locus_tag="AC158Q_06115"
     CDS             303..1250
                     /locus_tag="AC158Q_06115"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3339384.1"
                     /translation="MAVQYFFSAPARAAEAAFPARPIRFIVPFPPGTTTDVVARLVAL
                     RTGERLGQQIVVDNRAGASGTIGAELSARATPDGYTWGLGTTTTHALAAILNPRLGYD
                     PVRDFAPVSLLGDSPYFLTTYPGLPASNVREFVAHARANPGKLHYASVGNFSVGHLTG
                     ELLKKAAGIDMAHVPYKGSNLALIDLLAGRIQMNISTIAASLPHVKAGKLRSLGVTSL
                     KRIATLPQTPTIAESGYPGFQSSLWVGAFVPARTPGALVARLNREINATLEVVEVRSA
                     LADQAFEPGAVTPAAFTRLIKEETARWRKVIQDAGLKPE"
     gene            complement(1392..1916)
                     /locus_tag="AC158Q_06120"
     CDS             complement(1392..1916)
                     /locus_tag="AC158Q_06120"
                     /inference="COORDINATES: protein motif:HMM:NF024675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HIR3339385.1"
                     /translation="MNLYLRLFLLLFRLIGLPRKGLLEESRVAFRVLPSDCDINFHMN
                     NGRYLTFMDLGRVHLVTQVGLMPVIIRKRWRAALAAAEVNFIRPLAPFRKFDLVTRIV
                     TWDEKYCYMEQRFESEGSLCAHAFVKGLFLDAGGRVANSAVVVELGYSEPPPPMPEEL
                     RIWAELGSVKKQKK"
     gene            complement(2019..2657)
                     /locus_tag="AC158Q_06125"
     CDS             complement(2019..2657)
                     /locus_tag="AC158Q_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008112221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HIR3339386.1"
                     /translation="MDLLTGFFDIVLHLDRHLQWLVANYGAWIYAILFLIIFCETGLV
                     VTPFLPGDSLLFVAGTIAAAGDMYVHGLFAALAAASFLGDNTNYWIGRYAGPRVFTRE
                     GSRFLNPAHLARTQRFYDKYGANTIFFARFMPIVRTFAPFVAGIGRMRYLRFLLYSFS
                     GSIAWVGSLTYAGYYFGNVPVVKQNLTLVIVFIVLLSITPGVVEYFRHRSRS"
     gene            complement(2759..>3657)
                     /gene="secF"
                     /locus_tag="AC158Q_06130"
     CDS             complement(2759..>3657)
                     /gene="secF"
                     /locus_tag="AC158Q_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HIR3339387.1"
                     /translation="LFNVISIVTFLVAVGSLATRGLNLGVDFTGGTVMEVAYKQPADL
                     GKIREVMARLGFTEASVQNFGTSRDVMIRLPVKQGVTNAKLSEQVIQELRKSDSSVEM
                     RRVEFVGPQVGSELLEAGALALLFVSIGIVVYLALRFEWKFGVATIIANLHDGVIIVG
                     VFSLFQLEFSLPVLAAVLAILGYSVNESVVVFDRVRETFRNRRMRSLSVPQVLDHAIT
                     ATMSRTIITHGCTQLMVVAMLIFGGETLFHFALALTIGILFGIYSSVLVASPLVMWLR
                     VTREDLARPEAARKPGEDGAVV"
BASE COUNT          737 a         1181 c         1120 g          619 t
ORIGIN      
        1 gacgatcccc tgctcggctt tcgcaacgtg atcatcacgc cgcaccttgc cgccgcgccc
       61 cgctacaatg cgctcgaaga cttcgaggag cttgtgctcg ggctcgacac tgctttgaaa
      121 ctgtcaaatt aaccgtcaag cttgtcctga gcgtgtcgaa gggacgtcaa ggctgccagg
      181 aattcaaaat gaggagtctc ggacctatgc caataaacgc gggcgagaac agaaatagcg
      241 gcggtaacgc gaaccgattg tgttctcggt ccgtattgtt cttccttggc ggccttggcg
      301 tcttggcggt tcagtacttt ttttcggcac ccgcgcgggc agcggaagcg gcatttcccg
      361 cgcggcccat ccgcttcatc gtccccttcc cgccgggaac gacgaccgac gtcgtggcgc
      421 gactcgtggc actgcgcacc ggcgagcgcc tgggccagca gatcgtggtc gacaaccgcg
      481 cgggcgcgag cggcacgatc ggggccgaac tgtccgcgcg cgccacccct gacggctaca
      541 cgtgggggct cggcacgacc accacccatg cgctggcggc catactgaac cccaggctgg
      601 gctacgaccc ggtccgggat ttcgcgccgg tttcactgct gggcgattcg ccttatttcc
      661 tgacgactta tcccgggctg ccggcgtcca acgtccggga gttcgttgcc catgcgcgcg
      721 ccaatcccgg caagctgcac tacgcctccg tcggcaattt cagcgtcggg catctcaccg
      781 gcgagctgct caagaaggcg gccggcatcg acatggcgca cgtcccttac aaggggtcca
      841 acctggcgct gatcgatctc ctggccgggc gcatccagat gaacatcagc accatcgcgg
      901 cgtcgctgcc ccacgtaaag gccggcaagc tgcgctcgct gggcgtcacc agcctgaaac
      961 ggatcgccac gctgccgcag acgccgacca tcgcggaatc gggctatccc ggtttccagt
     1021 cctcgctgtg ggtgggcgca ttcgttccgg cacgcacgcc cggtgcgctg gtcgcgcgcc
     1081 tgaaccgcga gatcaacgcg acgctggaag tcgtcgaagt gcgcagtgcg ctggctgacc
     1141 aggctttcga gcccggcgcc gtcacccccg ccgccttcac gaggctcatc aaggaagaaa
     1201 ccgcgcgctg gcgcaaggtc attcaggacg cgggcctcaa gcccgaataa ccctgacaac
     1261 gggtggctcg tttctcgttg ttcgttgctc gttgctcgtg atttcccgcc gggaaccctg
     1321 acaggctccc aggctgaatc agcgggcgga gtttcaatgc ttcagaccag aaacgaaaaa
     1381 cgagcaacga gtcacttttt ctgtttcttg acgctgccca gctcggccca gatcctcaac
     1441 tcctcgggca tcggcggtgg cggttcggag tagcccagtt ccactaccac cgccgagttc
     1501 gccaccctgc ccccggcgtc caggaacagc cctttcacga aagcgtgcgc gcacagcgag
     1561 ccttccgact cgaacctctg ctccatgtag cagtacttct cgtcccaggt gacgatgcgc
     1621 gtcaccaggt cgaacttccg gaacggggcg agcggacgga tgaagttgac ctcggccgcc
     1681 gcgagcgccg cgcgccagcg tttgcggatg atgacgggca tcagcccgac ctgggttacc
     1741 aggtgcaccc gccccaggtc catgaacgtc aggtagcgcc cgttgttcat gtgaaaattg
     1801 atgtcgcagt cgctgggcag cacgcgaaac gcgacacgcg actcctcgag caaccccttg
     1861 cgcggcagcc cgatcaggcg gaacagcagc aggaacaacc gaagatacaa attcataata
     1921 gacaggattt acaggattag actggaacaa aaaacaaaaa gggttcagat tctctgctta
     1981 aatcttgtta atcttgttaa tcctgtccat tgcgtttgtt acgagcgaga gcggtggcgg
     2041 aagtactcga cgacgccggg agtgatcgag agcagcacga tgaaaacgat caccagcgtc
     2101 aggttctgct tcaccaccgg cacgttgccg aagtaatagc cggcgtaggt gagcgagccg
     2161 acccacgcga tgctgcccga gaaactgtag agcaggaaac gcaggtagcg catgcgcccg
     2221 atgcccgcga cgaaaggcgc gaacgttcgg acgatgggca tgaagcgggc gaagaatatc
     2281 gtgttcgcgc cgtatttatc atagaagcgc tgggtgcgcg ccaggtgcgc cggattcagg
     2341 aagcgagagc cttctcgcgt gaacacgcgt ggaccggcgt aacgcccgat ccagtaattc
     2401 gtgttgtccc cgaggaatga cgccgccgcg agcgcagcga acaggccgtg cacgtacatg
     2461 tccccggccg cggcgatcgt gcccgcgacg aacagcagcg agtcgcccgg caggaacggc
     2521 gtcaccacca ggcccgtctc gcagaagatg atgagaaaca ggatcgcgta gatccacgcg
     2581 ccgtaattcg cgaccagcca ctgcaggtgc cggtcgaggt gcaggacgat gtcgaaaaag
     2641 ccggtgagga gatccacgtt atgccgctcc agctatcgcg gactgtcggc gtcaaccggc
     2701 cgaagcatgc ggcggcgcgg ggatccgggc gtcgtgaccg ctcaaggctg gagcgccgtc
     2761 agacgaccgc tccgtcctcg ccgggctttc ttgccgcttc cggcctggcc aggtcctcgc
     2821 gcgtcacccg cagccacatc acgagcgggc tcgccaccag caccgaggaa tagatgccga
     2881 aaaggatccc gatggtgagc gcaagcgcga agtgaaacag cgtctcgccg ccgaagatca
     2941 gcatcgcgac caccatcaac tgcgtgcagc cgtgagtgat gatggtgcgc gacatggtcg
     3001 ccgtgatcgc gtgatcgagc acctgcggca cggaaagact gcgcatccgg cgattgcgga
     3061 atgtctcgcg caccctgtcg aacaccacca ccgactcgtt gaccgagtag ccgaggatcg
     3121 ccagaaccgc ggcgagcacc ggcagcgaga attccagctg gaacagcgag aaaacgccga
     3181 cgatgatcac gccgtcgtga aggttggcga tgatcgtcgc cacgccgaac ttccactcga
     3241 accgcaacgc gaggtagacg acgatgccga tcgagacgaa cagcagcgcg agcgctcccg
     3301 cctcgagcag ctcgctgccg acctgggggc cgacgaactc gacgcgtcgc atctcgaccg
     3361 aggaatcgga ctttctcagt tcctgtatca cctgctcgga gagcttggcg ttcgtcacgc
     3421 cctgcttcac gggcaggcga atcatcacat cgcgcgaggt tccgaagttc tgcaccgagg
     3481 cctcggtgaa ccccagtcgc gccatcactt cgcggatctt gccgagatcc gcgggctgct
     3541 tgtaggccac ttccatcacc gtgccgccgg tgaaatccac gccgaggttg agaccgcgcg
     3601 tggcaaggga gcccacggca accaggaacg tgacgatgga gatcacgttg aagagca
//
LOCUS       DBNGSG010000231         3425 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1094038, whole genome shotgun sequence.
ACCESSION   DBNGSG010000231 DBNGSG010000000
VERSION     DBNGSG010000231.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1094038"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="AC158Q_06135"
     CDS             complement(<1..190)
                     /locus_tag="AC158Q_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HIR3339388.1"
                     /translation="MLIEVKVPQLSESVAEATLLAWHKKPGDTVKRDENLVDIETDKV
                     VLETPAPDSGVIVKIIKGD"
     gene            complement(215..3067)
                     /locus_tag="AC158Q_06140"
     CDS             complement(215..3067)
                     /locus_tag="AC158Q_06140"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HIR3339389.1"
                     /translation="MMKQMLGNSYLFGANAPFIEALYEAYLKDPASVEARWRDYFDEL
                     QRLDRGVTDVSHFDVQERFAQLAKSGRAATPTVVINRPQLGEKQFAVLQLISAYRILG
                     GRLAEIDPLKRRERPHTPELDPAFYGLGEADMETVFNTGTLVAPREMKLKDIVQFLKD
                     TYCRSVAVEYMHVTDMTQKRWVQERLESVRAAASYDAAYKKHILERLTAAETLEKYLH
                     TRYVGQKRFSLEGGETLIPQLDNLLQGAGKAGVQEMVIGMAHRGRINVLVNTMGKMPK
                     DLFAEFEGKHHDDLLAGDVKYHQGFSSNVMTPGGPMHVTLAFNPSHLEIVNPVVEGSV
                     RARQHRRGDKTGDQVLPVLIHGDAAFAGQGVNMETFEMAQTRGYGTGGTLHLVVNNQI
                     GFTCSDLRDARSTLYCTDIAKMLEIPIFHVNGDDPEAVCFATEIALEYRMKFHKDVVV
                     DLVCYRRLGHNEQDEPMVTQPLMYKTIAKLPTTRKLYADQLSKEGVITESGGDELVAM
                     IRKALDDGHHTNKTILSNHKPPFEIDWSQYRGGKWTEHDDTRLPAETLKALGERITRV
                     PDNFKLHPAVQRVMESRRLMAQGKLPLDWGMAEHLAFATLLNEGYSIRLSGQDSGRGT
                     FFHRHAVLHDQNREKWDQGTYVPLQHVGEKQGDFVVIDSLLSEEAVMGFEYGYSTSAP
                     NELVIWEGQFGDFANGAQVVIDQFIASGEAKWGRLCGLVLMLPHGYEGQGPEHSSARI
                     ERYLQLCADYNMQVCIPATPVQLYYLLRRQMIRPYRKPLVLFTPKSLLRHKESVSPLE
                     EFAADKFNAVNEDWESKDINSDNVKRVVFCSGKVYYDLRAERKSKGLKDLPLVRIAQL
                     YPFPHEDLQAQIEHYRKATEVVWCQEEPRNQGAWHRIQHYLERHLLPHQNLIYAGRDS
                     SASPAAGYKSLHDRQQKELVELALSVGVASRADGIK"
     gene            complement(3159..>3425)
                     /locus_tag="AC158Q_06145"
     CDS             complement(3159..>3425)
                     /locus_tag="AC158Q_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HIR3339390.1"
                     /translation="ALEKVALEDEYFVKRKLYPNVDFYSGIVYKALGIPVAMFTAIFA
                     LARTVGWIAQWKELISDPDQKIGRPRQLFTGATRREFVPMGKRK"
BASE COUNT          576 a         1094 c         1048 g          707 t
ORIGIN      
        1 catcgccctt gataatcttc acgatcacgc ccgagtccgg cgccggcgtt tcgagcacca
       61 ccttgtcggt ctcgatgtcc accaggtttt cgtcgcgctt gacggtgtcg cccggcttct
      121 tgtgccatgc gagcagcgtg gcttcggcta ccgactcgga cagctgcggt accttgacct
      181 cgatcagcac aaataatcct taactatcat cttgttattt tataccgtcg gcgcgagatg
      241 cgacgcccac gctcaacgcg agctcgacca gctctttttg ctgccggtcg tgcagcgact
      301 tgtacccggc ggccggcgag gcggacgaat cgcgccccgc gtaaatcagg ttctggtgcg
      361 gcagcaggtg gcgttcaaga tagtgttgga tgcggtgcca ggcgccctgg ttgcgcggct
      421 cctcctgaca ccagacgacc tcggtcgctt tccggtagtg ctctatctga gcctgcaaat
      481 cctcgtgcgg aaaggggtaa agctgggcga tgcgcaccag cgggaggtcc ttcaaccctt
      541 tgctcttccg ctccgcacgc aggtcgtaat acaccttgcc gctgcagaat accacccgtt
      601 tcacgttgtc ggaattgatg tctttcgatt cccaatcctc gttgaccgcg ttgaacttgt
      661 cggcggcaaa ttcctccagc ggcgacaccg attccttgtg acgcagcagg ctcttgggcg
      721 tgaacaggac gaggggcttg cggtacgggc gtatcatctg gcgccgcagc agatagtaga
      781 gctgcaccgg cgtcgccggt atgcagacct gcatgttgta gtccgcgcaa agctggagat
      841 agcgctcgat gcgcgcggac gaatgctccg gcccctgccc ctcgtagccg tgcggcagca
      901 tcaggaccag cccgcacagg cggccccact tggcctcgcc ggaagcgatg aactggtcga
      961 tgactacctg ggcgccgttg gcgaaatcgc cgaattgccc ttcccagatc accagctcgt
     1021 tgggcgccga ggtggaatag ccgtactcga aacccatgac ggcctcttcc gagagcagcg
     1081 agtcgatgac cacgaagtcg ccctgcttct cgccaacgtg ctgcagcggc acgtaggtgc
     1141 cctgatccca tttctcccgg ttctggtcgt gcagcacggc atggcggtga aagaacgtgc
     1201 cgcggccgct gtcctggccc gagagccgga tcgagtagcc ttcgtttaac agcgtggcga
     1261 atgccaggtg ctcggccatg ccccagtcga gcggaagctt gccctgcgcc atcaggcggc
     1321 ggctctccat gacccgttgc accgcagggt ggagcttgaa attatccggc acccgggtga
     1381 tgcgctcacc cagcgccttc agggtctcgg ccggcaggcg ggtatcgtca tgctcggtcc
     1441 acttgccgcc gcggtactgg ctccagtcga tctcgaacgg aggcttgtgg ttggagagga
     1501 tggtcttgtt ggtgtggtga ccgtcgtcga gcgccttgcg gatcatggcg accagctcgt
     1561 caccgcccga ttctgtgatc accccttcct tcgacagctg gtccgcgtag agcttgcggg
     1621 tggtgggcag tttcgcaatc gtcttgtaca tcagtggctg cgtcaccatc ggctcgtcct
     1681 gctcgttgtg gccgagccgg cggtagcaca cgagatccac caccacgtcc ttgtgaaact
     1741 tcatgcggta ctcgagcgcg atttcggtcg cgaagcacac cgcctcggga tcatcgccgt
     1801 tgacatggaa gatcggtatt tccagcatct tggcgatgtc ggtgcagtag agcgtcgagc
     1861 gcgcgtcgcg caggtccgaa caggtaaagc cgatctggtt gttgacgacg aggtggagcg
     1921 tgccgccggt accgtaaccc cgcgtctgcg ccatctcgaa cgtttccata ttcacgccct
     1981 gccccgcgaa tgcggcatcg ccgtgaatca gtaccggcag cacctggtcg ccggtcttgt
     2041 cgccgcgccg gtgctggcgc gcccgcaccg agccttcgac cacgggattg acgatctcca
     2101 ggtgtgacgg attgaacgcc agggtcacat gcatcgggcc gcccggcgtc atgacgttgg
     2161 acgaaaaccc ctggtgatac ttgacgtccc cggcgaggag atcgtcgtgg tgcttgccct
     2221 cgaattccgc gaacaggtcc ttgggcatct tgcccatggt gttgaccagc acgttgatgc
     2281 gcccgcggtg cgccatcccg atcaccatct cctgcacccc ggccttgccc gcgccctgga
     2341 gcaggttgtc gagctgcggg atgagcgttt cccctccttc gagcgaaaaa cgcttctgcc
     2401 cgacatagcg ggtatgcagg tacttctcca gcgtctcggc cgcggtcagg cgctcgagga
     2461 tgtgcttctt gtacgcggcg tcgtaggacg ccgccgcgcg aacgctctcc agccgctcct
     2521 ggacccaccg cttctgcgtc atgtcggtaa catgcatgta ctcgacggct acgctgcggc
     2581 aataggtatc cttcaggaac tgcacaatgt ccttcagctt catttcccgc ggcgcgacca
     2641 gcgtgccggt gttgaacacc gtctccatgt ccgcttcgcc cagcccgtag aacgcggggt
     2701 cgagctcggg cgtatgcggc ctctcgcggc gcttgagcgg atcgatttcg gccaggcgtc
     2761 cgcccaggat gcggtaggcg gagatgagct ggagcacggc aaactgcttc tcgccaagtt
     2821 gcggccggtt gatcactacc gtcggtgttg ccgcgcgccc cgattttgcc aattgcgcga
     2881 aacgctcctg cacgtcgaag tgggatacgt ccgtcacgcc ccggtccagg cgctgcagtt
     2941 cgtcgaaata gtcgcgccag cgcgcctcga ccgaagccgg gtccttgagg taggcctcat
     3001 agagcgcctc gatgaacggc gcattggcgc cgaatagata ggaattgccg agcatctgct
     3061 tcatcatgac gtcacctcac ggttccgagt gtcgaggatg gagtgtcgag ggatgccgca
     3121 agttcatacc cctcgacacc cgacactcgt ccctcgactc acttgcgctt gcccatgggg
     3181 acgaactcgc ggcgggtagc gccggtgaag agctgccgcg ggcgcccgat cttctggtcg
     3241 gggtccgaga tcagttcctt ccactgggcg atccagccca ccgtgcgcgc gagcgcgaag
     3301 atcgccgtga acatggcgac cggtattcca agcgccttgt agacgatgcc cgagtagaaa
     3361 tccacgttgg gatagagctt gcgtttgacg aagtactcgt cctcgagcgc cactttttcc
     3421 agcgc
//
LOCUS       DBNGSG010000232         1080 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1262501, whole genome shotgun sequence.
ACCESSION   DBNGSG010000232 DBNGSG010000000
VERSION     DBNGSG010000232.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1262501"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="AC158Q_06150"
     CDS             complement(<1..496)
                     /locus_tag="AC158Q_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR3339391.1"
                     /translation="MRRLKTDILILGSGGAGLFAALHAHQVDPKLSITIAVKGLLGKC
                     GCTRMVQGGYNVALAPQDSVERHFMDTIEGGKWLPDQELAWALVEGAIERIRELENEL
                     GCFFDRNADGTVHQKAFAGQTFDRTVHKGDLTGIEIINRLAEQVWARGIGRLEEHRAI
                     EFIRS"
     gene            complement(659..>1080)
                     /locus_tag="AC158Q_06155"
     CDS             complement(659..>1080)
                     /locus_tag="AC158Q_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HIR3339392.1"
                     /translation="AGETEANPTGYAPICIGTTTSDRMERFTRPLMQEYGARIIIGKG
                     GLREGSAAAFRDLGGVYLAVIGGAAALETTWIEQIEDVDLDDLNPESLWKFRVKGFGP
                     LLVSMDSHGGSLYTEVNTAARSKRAAALASLGVKTGN"
BASE COUNT          190 a          356 c          327 g          207 t
ORIGIN      
        1 ggctcctgat gaactcgatc gcgcggtgtt cctccagccg cccgatgccg cgcgcccaga
       61 cctgctcggc gaggcggttg atgatctcga tgccggtgag atcgcccttg tgcacggtgc
      121 ggtcgaacgt ctgccccgcg aacgccttct ggtggacggt gccgtcggcg ttgcggtcga
      181 agaagcagcc gagctcgttc tcgagctcgc ggatgcgctc gatcgcccct tccaccaggg
      241 cccaagccag ctcctggtcg ggcagccact tgccgccctc gatcgtgtcc atgaaatggc
      301 gctcgaccga gtcctgcggc gcgagcgcga cgttgtagcc gccctgaacc atgcgcgtgc
      361 agccgcactt gcccagcagc cccttcaccg cgatggtgat cgagagcttc gggtcaacct
      421 ggtgcgcgtg cagcgcggca aacagccccg cgccgcccga gccgagtatc aggatgtccg
      481 tctttaacct tcgcattaaa ttagccgcca agacgccaag aacgccaaga gaatctatac
      541 aaaataggag aacaaaaacg aaaaaaagaa taaaggaaaa ctatggaact cgaattgttt
      601 ccgatgtgtc cggctgtttg ataattatat tttcttggcg gccttggcgt cttggcggct
      661 aatttccggt tttcacgccg agcgacgcga gggccgcggc gcgcttcgag cgcgcggccg
      721 tgttcacttc cgtgtagagg ctgccgccgt gagaatccat cgacaccagc agcgggccga
      781 agccctttac gcggaacttc cacagcgatt ccggattgag gtcgtccaga tccacgtcct
      841 cgatttgctc gatccaggtg gtttccagcg ccgccgcgcc gccgatcacc gccaggtaga
      901 cgccacccag gtcccggaac gccgccgccg agccttcgcg cagtccgccc ttgccgatga
      961 tgatgcgcgc gccgtattcc tgcatcagcg ggcgcgtgaa ccgctccatg cggtcgctgg
     1021 tggtggtgcc gatgcagatc ggcgcgtagc cggtcgggtt cgcttccgtt tcgcccgccc
//
LOCUS       DBNGSG010000233         2518 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1942476, whole genome shotgun sequence.
ACCESSION   DBNGSG010000233 DBNGSG010000000
VERSION     DBNGSG010000233.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1942476"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="AC158Q_06160"
     CDS             complement(<1..794)
                     /locus_tag="AC158Q_06160"
                     /inference="COORDINATES: protein motif:HMM:NF004781.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HIR3339393.1"
                     /translation="MDVPTATEKLIARKDGAIGWVIFNNPQKRNAMSLEMSEATAAAM
                     DSYAKDPDVRVVILRGEGDKAFVSGADISQFKERRSNMEQVRAAEAVSERTSKAIREC
                     PKPVIAMIRGYCMGGGLGLAVACDIRIAADDSRFGVPAARLGVGYRFSGIRRLAETVG
                     PQFTAEIFYTGRQFTAQEALQMGLINRLLPAAELEQYVMDFAATLAGNAPLTLAAVKK
                     CLIELRKDPADRDLDRCQQMVEACFASEDYIEGRNAFMEKRKPAFKG"
     gene            complement(959..2026)
                     /locus_tag="AC158Q_06165"
     CDS             complement(959..2026)
                     /locus_tag="AC158Q_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3339394.1"
                     /translation="MLIFRQLFDQQSSTYTYLLADPGSREAVMIDPVFEQARRDQALI
                     GELGLKLLWTLETHVHADHVTGAWLHKQRLGSRIAIAAASGAQGADRMLVQGDRIEFG
                     RRALEVRATPGHTNGCQTYVMDDRSMAFTGDALLIRGCGRTDFQQGSAHTLYRSVREQ
                     IFTLPDACLVYPGHDYRGLAASSVGEERQFNPRLGGQILVEDFAGYMTNLGLAHPRQI
                     DIAVPANLRCGRPAQAVRPDPGWAPLTFNFSGIWEVQPDWLEEHADEVQVIDVREPDE
                     FNGAFGHVPGARLVPLGSLAARAGELSKERPIVTVCRSGARSAQATVLLARAGFEQVA
                     NLAGGMLRWRAQGYRVEGGSD"
     gene            complement(2164..>2518)
                     /locus_tag="AC158Q_06170"
     CDS             complement(2164..>2518)
                     /locus_tag="AC158Q_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339395.1"
                     /translation="TPDGELSASVQEGCTAYCVAYLWIVPAQVGGAWMLADGELMLNQ
                     AFQRVTGTLRAGGQETAVTGRLTGDQLSFEAGGVRYTGRVGGNVIRGTATSGAGASGW
                     SAARAAKAAAVAAGS"
BASE COUNT          397 a          901 c          795 g          425 t
ORIGIN      
        1 cccttgaacg ccggcttgcg cttctccatg aaagcattgc ggccctcgat atagtcctcg
       61 ctcgcgaagc acgcttcgac catctgctgg caccggtcga ggtcgcggtc agcggggtcc
      121 ttgcgcagct cgatcaggca tttcttgacc gccgcgagcg tgagcggcgc gttgccggcg
      181 agcgtggcgg cgaaatccat cacgtactgt tccagctcgg cagcgggcag caggcggttg
      241 atcaggccca tctgcagcgc ttcctgcgcc gtgaactgcc gcccggtgta aaaaatctcc
      301 gcggtgaatt gcggccccac ggtctccgcc agccgccgta tgcccgagaa gcggtagccc
      361 acgcccagcc tggccgcggg cacgccgaaa cgcgagtcgt ccgcggcgat gcggatatcg
      421 cacgccaccg cgaggcccag gccaccgccc atgcagtagc cgcggatcat ggcgatcacc
      481 ggcttggggc attcgcgtat cgccttgctg gtccgctccg agaccgcctc ggccgcgcgc
      541 acctgctcca tattggagcg ccgctccttg aactgcgaga tgtccgcgcc ggagacgaag
      601 gccttgtcgc cctcccccct caggatcacc accctgacgt cgggatcctt cgcatagcta
      661 tccatcgccg ccgccgtcgc ctccgacatt tcgagcgaca tcgcattgcg cttttgggga
      721 ttattgaaaa taacccaacc tattgcaccg tccttgcggg cgatgagttt ctcggttgca
      781 gttggcacgt ccatttgctt ctccttgaag gcgtgaatcg tgagtcgtga ttcgaccatg
      841 aaccggatat gagccgctag ggtaaatgaa aaagccccgc cgcagcgggg cttctcaata
      901 cgtggacggg gttcgtgacc ttgcgcttcc cgcgatacgc cgttcgcctt cgacgaagtc
      961 agtcgctgcc cccttcgacc cggtatcctt gcgcgcgcca gcgcagcatc ccgcccgcga
     1021 ggttcgcaac ctgttcgaat cccgctctgg cgagcagcac ggtggcctgc gccgatcgcg
     1081 ccccggagcg gcacacggtc acgatcggcc gctccttcga caactcgccc gcccgcgccg
     1141 ccaggctgcc gaggggcacc aggcgcgcgc ccggcacgtg accgaacgcc ccgttgaatt
     1201 cgtcgggctc gcgcacgtcg atgacctgga cttcatccgc atgctcttcc agccagtcgg
     1261 gttgcacttc ccagatgccg gagaagttga aggttagcgg cgcccaaccc gggtccgggc
     1321 gcaccgcctg cgccggccgg ccgcagcgga ggttggccgg caccgcgata tcgatctgcc
     1381 tggggtgcgc caacccgaga ttcgtcatgt agccggcaaa gtcctccacc aggatctgcc
     1441 cgccgaggcg cggattgaac tggcgctcct cgccgacgct gctggccgcg agcccccgat
     1501 agtcgtggcc cgggtagacc aggcaggcgt cgggcagcgt aaatatctgc tcgcgcaccg
     1561 agcggtagag cgtgtgcgcg ctgccctgct ggaagtcggt gcgcccgcag ccgcggataa
     1621 gcagtgcgtc gccggtaaac gccatcgagc ggtcgtccat cacgtaagtc tggcaaccgt
     1681 tggtgtgccc gggggtggcg cgcacttcga gcgcccgccg gccgaactcg atgcggtcgc
     1741 cctgcacgag catccggtcc gcaccctggg cgccagacgc cgccgcaatc gcgatgcggc
     1801 tgcccagccg ttgcttgtgc agccaggccc ccgtgacgtg gtcggcatgc acgtgcgtct
     1861 ccagcgtcca cagcagcttc agtccgagtt ccccgatcag cgcctggtcg cggcgcgcct
     1921 gctcgaacac gggatcgatc atcaccgcct cgcgcgaccc ggggtcggca agcaggtagg
     1981 tgtaggtcga ggactgctgg tcgaagagct gcctgaaaat aagcattgcc acgccgcgag
     2041 tagtaataga aaacagctta cccctcccgt gccgggcccg gcaatgcggc tcgcgcgtaa
     2101 cgagggtggc gtccaccctc accgttcggt atcaggccag agggaaaata cgtcgcccgg
     2161 ggttcaggac ccggcagcca cggcagccgc cttcgcggcc cgcgccgcgc tccacccgga
     2221 ggcgccggcg ccggacgtgg cggtgccccg gatgacgttg ccgccgacgc gcccggtgta
     2281 gcggacgccg ccggcctcga agctcagttg atcgccggtg agtcgtcccg tcacggcggt
     2341 ttcctgcccg ccggccctga gcgtgccggt gaccctctga aacgcctggt tcagcatcag
     2401 ttcgccgtcc gccagcatcc acgcgccccc gacttgcgcc ggcacgatcc agaggtacgc
     2461 cacgcaatac gcggtgcagc cttcctgcac gctggccgac aattccccgt ccggcgtc
//
LOCUS       DBNGSG010000234         3675 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3810548, whole genome shotgun sequence.
ACCESSION   DBNGSG010000234 DBNGSG010000000
VERSION     DBNGSG010000234.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3810548"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1243)
                     /locus_tag="AC158Q_06175"
     CDS             complement(<1..1243)
                     /locus_tag="AC158Q_06175"
                     /inference="COORDINATES: protein motif:HMM:NF015410.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3339396.1"
                     /translation="MKSLPPNPAAESLAAAALAFLRRYPPFDSMEDEALRFLASRLAL
                     AYYPKDAVIFAPGHGKPQHLHLVQRGVVRCEDGSQPGNPSLATLQPGECFPIDALLER
                     LPGDSSYTASADTFCYQLPAADFDELLHRSPRFQEFATRALASLLRESRRLLAMHSAS
                     LAGEQQAMNRELRSLVKRAPVSCAPDTTIGAALRAMAQARTGSILVVNRDGALAGILT
                     RHDVLDRIALARRDLEAPVGDVMTPHPRTLSAEASAYDAALLIAREGFRHIPVMDGGT
                     LIGIVTERDLFALQHASARGINRTISRAAEPGALRHAAGEIHELARGLLAQGLAAEQL
                     TTIISTLNDALTRRAIEIERPRHALDGIEWCWLAFGSEGRLEQTISTDQDNGIVFADA
                     AGRAPDDFRARLLPFAQAVNRM"
     gene            complement(1503..2582)
                     /locus_tag="AC158Q_06180"
     CDS             complement(1503..2582)
                     /locus_tag="AC158Q_06180"
                     /inference="COORDINATES: protein motif:HMM:NF015552.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance/C4-dicarboxylate
                     transporter family protein"
                     /protein_id="HIR3339397.1"
                     /translation="MSPPSAQTLAPPASLAGRIDQQIRQLYPGYFALVMATGIISNAA
                     LFLGHRVLSDSLLAVNFAAFPVLFLALALRAMRFRRDLWADLMNPRLVFSFFTIVAAS
                     DVFGLQLVLRGYTEPAVALWLFAFLAWTVLGYLSFGVLTFINAGSGADVVHGGWLIAI
                     VGTESLVLLGAQVAPHFGTLSSLAFVTIYALWGVGIVFYGIFITLFSYRLFFLRVEPS
                     DVTPLFWVVMGAAAIATNAGSTLILTDPGMPFLLDLRPFLDGTTLILWAWSTWWIPML
                     VIFGVWKHLVRKEPIVYHAMFWSLVFPLGMYTLATWRLSLAADFPLLQLLPRIMIWVA
                     LGAWVLTMTGALAAFWRVLRPPVQP"
     gene            complement(2706..3158)
                     /locus_tag="AC158Q_06185"
     CDS             complement(2706..3158)
                     /locus_tag="AC158Q_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR3339398.1"
                     /translation="MRLTSFTDYTLRVLIYLGAHQREGRLSTIGDIAAAYGISENHLM
                     KVVHHLAREGHVETVRGKGGGMRLAHAPEKINIGAVVRGAEEDLALVECFQQGGQPCP
                     IASACTLSGLMERALGAFFEVLDRQTLADLLKPQAQFTQIFGGLDRRA"
     gene            3344..>3675
                     /locus_tag="AC158Q_06190"
     CDS             3344..>3675
                     /locus_tag="AC158Q_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015112 - nitrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3339399.1"
                     /translation="MATEKFKQWSVVTASTTAFTVCFMVWMMFAVIGIPIKETLGLNE
                     TQFGILVATPVLTGSLIRLPLGMWTDRFGGRIVFFVLMLSTVFPIWLISRATEFWHFL
                     VTGLFVGVA"
BASE COUNT          641 a         1130 c         1303 g          601 t
ORIGIN      
        1 gcatgcggtt gacggcttgc gcgaacggca agagacgcgc ccggaagtcg tccggagcgc
       61 gcccggcggc gtcggcgaag acaatgccgt tgtcctggtc ggtgctgatc gtctgctcga
      121 ggcgcccctc gctgccgaag gcgagccagc accactcgat cccgtcgagc gcgtgccgcg
      181 gccgctcgat ctcgatcgca cgccgggtga gcgcatcgtt gagggtcgag atgatcgtgg
      241 tcagctgctc cgcggcgagt ccctgcgcga gtaagccgcg cgcgagctcg tggatttcgc
      301 ccgcggcgtg gcgcagggcc ccgggttccg ccgcgcggga gatggtgcgg ttgatgccgc
      361 gcgcgctcgc gtgctgcagc gcgaacaggt cgcgttcggt gacgatgccg atcagcgtgc
      421 cgccgtccat gaccggaata tgcctgaagc cttcgcgcgc gatgaggagg gccgcatcat
      481 aggcgctggc ctcggcggac agcgtcctgg ggtgcggggt catcacgtcg ccgaccggcg
      541 cctcgagatc gcggcgggcg agcgcaatgc ggtcgagcac gtcgtgccgc gtcaggatgc
      601 cggcgagcgc gccatcccgg ttgacgacca gtatcgaacc ggtacgcgct tgcgccatcg
      661 cgcgcagggc cgccccgatg gtcgtgtccg gcgcgcagct caccggcgcc cgtttgacca
      721 gggagcgcag ctcgcggttc atggcctgct gctcgccggc gaggctcgcc gagtgcatcg
      781 cgagcaggcg ccgcgattcc cgcaacaggg aagcgagggc gcgcgtggcg aattcctgga
      841 agcgcgggct gcggtgcagg agttcgtcga agtcggcggc cggcagctgg tagcagaatg
      901 tgtcggcgga ggcggtgtag ctcgagtcgc cgggcagtct ttcgagcagc gcatcgatcg
      961 ggaagcattc ccccggctgc agcgtggcga gggagggatt gcccggctgg ctgccgtcct
     1021 cgcaccgcac cacgccccgc tgaaccaggt ggaggtgctg cggcttgccg tgcccgggcg
     1081 cgaagatcac ggcgtctttc gggtagtagg cgagggcgag gcgggacgcc agaaaacgca
     1141 gcgcctcgtc ttccatcgag tcgaatggcg ggtagcggcg caggaaggcg agcgctgcgg
     1201 cggcgagcga ttcggcggct ggattcggcg ggagcgattt cacgggatga gcgccagttg
     1261 tccggtcgat cttgcccaat tgtatgacgg ctgctgttgc ggaccctagc gggttgcgcc
     1321 ctcgcgggcc gccgctgtgg atctgaatcg acaggcaggg tttccgtttc cgccaggttc
     1381 ggcgcgcctt tgtgaagacc gtgttcgtca tcaccgttgc ggcttgccac atggtcctgc
     1441 tgctccgcgc gagctactga tcgagctgct gatggtggtg gggcagcgac gtgccgggcg
     1501 cgctagggtt gcacgggcgg gcgcagcacg cgccagaacg cggccagcgc tcctgtcata
     1561 gtcagcaccc atgcgccgag cgcaacccag atcatgatgc gcgggaggag ctgcaaaagc
     1621 gggaaatcag cggcaagcga gaggcgccaa gtggcgagcg tatacatgcc gaggggaaac
     1681 accaggctcc agaacatcgc gtgatagacg atcggctcct tgcgcaccag gtgcttccac
     1741 acgccgaaaa tgaccagcat tggaatccac cacgtgctcc aggcccacag aatcagtgtc
     1801 gtcccgtcca gaaacgggcg caggtcgagc aggaacggca tgccggggtc agtcaggatg
     1861 agcgtggagc ccgcgttggt cgcgatagcc gccgcgccca tcacgaccca gaagagcggc
     1921 gtaacgtcgg acggctcgac acgcaggaaa aacaggcgat agctgaacag cgtgatgaag
     1981 atgccgtaaa agacaatgcc gacgccccac agcgcgtaga tcgtcacgaa ggcgagcgac
     2041 gagagcgtcc cgaaatgcgg cgccacctgc gcaccgagca gcacgagcga ctcggtgccg
     2101 acgatggcaa tgagccatcc accgtgcacg acgtcggcac cgctgccggc gttgatgaaa
     2161 gtcagcacgc cgaagctgag gtagccgagc acggtccacg ccagaaacgc gaacagccac
     2221 agcgcgactg ccggttcggt gtagccgcga agcacgagtt gcaggccgaa gacgtcggag
     2281 gcggcgacga tggtgaaaaa tgagaacacc agcctgggat tcatcaggtc ggcccagagg
     2341 tcgcgccgga agcgcatggc gcgcagcgcc aaggcgagaa aaagcaccgg aaaggcggcg
     2401 aaattcacgg ccaacaggct gtctgagagc acgcggtgtc cgaggaacag cgcggcgttg
     2461 gagatgatgc ccgtcgccat tacgagcgcg aagtagcctg ggtagagctg gcggatctgc
     2521 tggtcgatgc ggccggccag ggacgcgggc ggggccaggg tttgcgcgga aggcgggctc
     2581 attatttcac cggggctgtg cgcgtttgcc ggattatcct taatcgtgac acggccgccc
     2641 acttgcagcg cgcgatcgtt tgcagggggc gtctggaggg ggcgacgcaa ggcttaatgg
     2701 aagcactatg cgcgtcggtc caagccgccg aatatctggg taaattgcgc ctggggcttg
     2761 agcagatccg ccagcgtctg gcggtccagc acctcgaaga acgcgccgag cgcgcgttcc
     2821 atgagcccgc tcagcgtgca ggcggacgcg atggggcagg gctgcccgcc ctgctggaag
     2881 cattccacca gggcgagatc ttcctcggcg ccgcgcacga cggcgccgat gttgattttc
     2941 tccggcgcgt gcgcgagccg catgccgccg cctttcccgc gcaccgtttc cacgtggccc
     3001 tcccgcgcca ggtggtgcac caccttcatc aggtgattct ccgagatgcc gtacgcggcc
     3061 gcaatgtcgc ctatggttga gaggcggcct tcgcgctggt gcgcaccgag gtagatgagc
     3121 acgcgcagcg tgtagtcggt gaagctcgtc agtcgcatgg tcttcctggc atggctttcc
     3181 ccggcgctgc gccggggccg ggccaagcac tgcacgaccc ggattctagg aggcttgcgc
     3241 attaagatgg atctgaaata catcttcatt tgacctagat caaaccgggc agggaccttc
     3301 ccccttaact tcttaccgga cgattcggcg gaaggggacg gtgatggcaa cggagaagtt
     3361 caagcagtgg tcggtggtga cggcgagcac gaccgcattc accgtctgct tcatggtgtg
     3421 gatgatgttt gccgtcatcg gcattcccat caaggagacc ctgggcctca acgagacgca
     3481 attcgggata ctggtggcga cgccggtgct gaccggctcg ctgatccgcc tgccgctggg
     3541 catgtggacc gaccgcttcg gcggacgcat cgtgttcttc gtgctgatgc tgtccaccgt
     3601 gtttccgatc tggctgatat cccgcgccac cgagttctgg cacttcctgg tcaccggcct
     3661 gttcgtgggc gtggc
//
LOCUS       DBNGSG010000235         7829 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2452420, whole genome shotgun sequence.
ACCESSION   DBNGSG010000235 DBNGSG010000000
VERSION     DBNGSG010000235.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7829)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7829)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7829
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2452420"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..984
                     /locus_tag="AC158Q_06195"
     CDS             55..984
                     /locus_tag="AC158Q_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010814566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR3339400.1"
                     /translation="MLEIRHLRTLIALHEAGTVSAAAKRVHLTQSALSHQLRAIEAHA
                     GVPVVKRKGQTVELTEAGQRLLALARTVAGEIQGAERDLARLAGKPSGTLRIVLECHT
                     CFDWLMPVMDAFRRNWPEVELDLVSGFHPDPFELLASDRADLVIGSEAKRRRGLVHAP
                     LFRYEIMAVLPTDHPLRSKRWLEPRDFKGQALITYPVPEERIDVIRRVLKPAGIRLQR
                     RTTELTVAILQLVASRRGLAALPNWGLKNYTDYEYVLARRIGRGGLWSDLYATMTKET
                     AGRPYASDFIETARARCFETLDGIKPLEKAVMK"
     gene            complement(1037..1699)
                     /locus_tag="AC158Q_06200"
     CDS             complement(1037..1699)
                     /locus_tag="AC158Q_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339401.1"
                     /translation="MRKAWIATIATAALLSPVFAASAQQQPPMSFFITSAGSGKGADL
                     GGLAGADRHCQSLATAVGAGKRTWRAYLSTSAEGGKPAVNARDRIGKGPWYSQDGRLI
                     ARDVKELHEGVNNINRMTALTEKGERVNNGASPPNMHDILTGSQPDGTAIAGDPGKTT
                     CGNWTMSGAGAAMVGHHDRFGLKVDEPSRSWNASHPSRGCSQDNLRSSGGAGLFYCFA
                     AK"
     gene            1844..3028
                     /locus_tag="AC158Q_06205"
     CDS             1844..3028
                     /locus_tag="AC158Q_06205"
                     /inference="COORDINATES: protein motif:HMM:NF022738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3095 domain-containing protein"
                     /protein_id="HIR3339402.1"
                     /translation="MTSERFYAELPVIIDFGAVTRPECYAPLPDDWHVALCDVRNSTL
                     AVQNGKYKNVNTVGAAAITAVLNAAGDLDIPFSFEGDGCVLCVPPRLLEDTRAALAKT
                     QQIARDSFGLDLRIATVPVTRIRESGYAIRVARIRVSEHYVQAAFAGGGIAHADRLIK
                     DPAMSRSYVIEPGSVAPRGSHDGLECRWQDIPSRHGETVCLMVRALSADAGRAAALYG
                     EVIASVRDIYGGDEACHPVALPALSLSLSGERLGNEVGVRAAGRGTPGKWLALMRTRW
                     VVLLGWFLMKFGIRSGDTDWGRYKETLVSNTDVRKFADIYRQVLAGTEAQRNALAAWL
                     EGRYARRELVYGLHVTDRAHMTCLVFNYAGRHLHFIDGADGGFFLAARAFKERAAKLA
                     GR"
     gene            complement(3073..3738)
                     /locus_tag="AC158Q_06210"
     CDS             complement(3073..3738)
                     /locus_tag="AC158Q_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR3339403.1"
                     /translation="MLAACFALGSQAQIAPSPAERAGYTGLLAAAAQGDAAQIRALTA
                     KGMKPDARDSHQRTPLHVAAYGKHHEAMRALAAAGANPNALERDRYDIVTIASVANDV
                     PTLKLALELGASAKNVTSRYDGTALIAAAHLGHAEVVRTLIRAGAPLDHVNNLGWTAL
                     IESIVLGDGGPRHTDTLRALVEAGASVNLADRNRRTPLALARARGSAYAEMVRILEAA
                     GAR"
     gene            complement(3842..4144)
                     /locus_tag="AC158Q_06215"
     CDS             complement(3842..4144)
                     /locus_tag="AC158Q_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339404.1"
                     /translation="MKPIGPVAVFAVLLSLPVLGLAQAKPAAAPAQPAAKPAAAPVQP
                     AAAPVQPEAKPAAAPAQPAVKLASGSAGRSRANEDARSCLELATNMEIHQCAEKYR"
     gene            complement(4224..4526)
                     /locus_tag="AC158Q_06220"
     CDS             complement(4224..4526)
                     /locus_tag="AC158Q_06220"
                     /inference="COORDINATES: protein motif:HMM:NF015735.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HIR3339405.1"
                     /translation="MLWAISCVDKPNTAAIREKVLQPHRDYLGSQKGILVLAGATQND
                     DGTQAIGSLFIVNVDSRAKAKAFSDGDPFTQNGVFSSITITRMRKGQWNPGAAEGA"
     gene            4548..5705
                     /locus_tag="AC158Q_06225"
     CDS             4548..5705
                     /locus_tag="AC158Q_06225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014601.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HIR3339406.1"
                     /translation="MVAERDYQIPDSGTAGAPVPHYNRHSIIISAAGFHEMAGKDRKI
                     AVLGCGAIGSSVSADLTMAGYDITVIDQWPEQVEALRAAGLHIGMKDGDVKTPPIRAL
                     HLCDLASANLEFDIVLLAVKSYDHRWLAEFIKPYLKTDGVVVGVMNGMNDDSIASIVG
                     RNRTVGCCIELSAEIFTPGQVQRNTTHQGTWFAVGELDGFYTPRVKEIQSILSHVARC
                     DVTNNIYGAKWTKLIANSMTMGPHGLLGLRNGEAAHLPGMADIAVKIGRESLAVGVAM
                     GYRIEPIFGLRADEFAGSSDENLVTARDTLMHHVGGNSRTAPIHDHIKGRRSEMDFIT
                     GIVTRKGKELGIPTPFNDAVLEIDRMINKAEIRMDRSNFDLLMGKLAPHMK"
     gene            complement(5823..7727)
                     /locus_tag="AC158Q_06230"
     CDS             complement(5823..7727)
                     /locus_tag="AC158Q_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HIR3339407.1"
                     /translation="MPNQKLLRVAVASAFAAIMLVTTGAHAAPGKTLTAFASEQEISD
                     LFKRWAAEARKRDAERRSTQAYQDASGMGALMSAAPASAKAAAEASADSITNVQHAGV
                     DEGGIVKRHGDHLVILRRGRLFTVKVGEELTPASVADAYGSGINPGGAWYDEMLISGN
                     TIAVIGYSYQRGGTEIGLFEISDAGRLRYRSTYHLRSNDYYSSRNYASRLIGDKLVFY
                     TPMHLNPWGGEPWGSFPAMRRWRPGATAAEFKPIAPATRIYRTDEPLDPHQGVALHTV
                     TVCDLAKAEMDCESTAVLGSPGRVFYVSGGSVYVWTTQGRRNRAASAPGSAVFRIPLD
                     GAAPTALKTAGSPIDQFSFFEGNDNHLNVLVRADGRGEGMWAAEANAGDFALLRVSLD
                     SFSDGRDAAPAEAYKPLPRPGGHAVQNRYVGPYLLYGTGTGWNRAQRMLHSQVYAVRY
                     ASGAGAQAIPLAHGVDRIEALGANAVIVGSDGRDLHFTSLRLAATASAQDRYTRKDAA
                     QGETRSHGFFYKPDSDNAGMVGLPVIGGGQSAARQLRHNSAAVLFLRNQGLRLSELGA
                     LDSQAAANANDGCRASCVDWYGNARPIFLGNRVFALMGYEIVEGRVAGSRIAEVQRVK
                     FSPVNVSLAR"
BASE COUNT         1217 a         2626 c         2693 g         1293 t
ORIGIN      
        1 gattatggtg taatatatga atattattca taattgacca agtccacgcg ccccatgctc
       61 gaaatccggc atctgcgcac cctgatcgcc ctgcacgagg ccggcaccgt gtcggcggcg
      121 gcgaaacggg tgcacctcac ccagtcggcg ctgtcccacc agctgcgcgc gatcgaagcg
      181 catgccggcg tcccggtcgt caagcgcaag ggccagacgg tggagctgac ggaagccggg
      241 cagcgattgc tcgcgctcgc gcgcacggtg gccggggaaa tccagggcgc cgagcgcgat
      301 ctcgcccggc tcgccggcaa gccgtccgga acgctgcgca tcgtgctcga atgccacacc
      361 tgcttcgact ggctgatgcc ggtgatggac gcgttccgcc gcaattggcc ggaggtcgag
      421 ctcgacctgg tctcgggttt ccaccccgat cccttcgagc tgctcgcttc ggaccgcgcc
      481 gacctggtga tcggctccga ggccaaacgg cggcgcggcc tcgtgcacgc gccgctgttc
      541 cgctacgaga tcatggccgt gctgccgacc gatcacccgc ttcgctcgaa gcgctggctg
      601 gagccgcggg atttcaaggg acaggcgctg atcacgtacc cggtgccgga ggagcgcatc
      661 gacgtgatcc ggcgcgtgct gaagccggcg ggaatccgcc tgcagcgccg cacaacggaa
      721 ctcacggtcg ccatcctgca gctggtggcg agccgccgcg ggctggccgc gctgccgaac
      781 tggggcctca agaactacac ggactacgag tacgtcctgg cccgccgcat cgggcgcggc
      841 gggctgtgga gcgatctcta cgcgactatg acgaaggaga cggcgggccg gccgtacgcc
      901 tccgacttca tcgaaaccgc ccgggcacgg tgtttcgaga cgctggacgg gatcaagccg
      961 cttgagaagg cggtgatgaa gtgacgaagt gacggggtga ggaggggtga actcctcatc
     1021 cctcatccct ccgccctcac tttgcggcga agcagtagaa cagcccggcg ccgcccgagg
     1081 atcgcaggtt gtcctggctg cagccgcgcg agggatgcga ggcgttccag gaacgggagg
     1141 gttcatcgac tttcaggccg aaccggtcgt ggtggccgac catcgcggcg ccggccccgc
     1201 tcatcgtcca gttgccgcag gtcgtcttgc cggggtcgcc cgcaatcgcc gtgccgtccg
     1261 gttgcgagcc cgtcaggatg tcatgcatgt tgggcgggct cgcgccgttg ttgacccgct
     1321 ctcctttttc ggtcaacgcc gtcatccggt tgatgttgtt gacgccctcg tgcaattcct
     1381 tgacatcccg cgcgatgagc cggccgtcct ggctgtacca cggccccttg ccgatgcggt
     1441 cgcgcgcgtt cacggcgggc ttgccgcctt ccgcgctggt gctgaggtag gcgcgccacg
     1501 tgcgcttgcc ggcgcctacg gcggtcgcga gcgactggca gtgccggtcc gcgccggcca
     1561 agccgccgag gtccgcgccc ttgccggatc ccgcgctggt gatgaagaac gacatggggg
     1621 gttgctgttg cgcgctggcc gcgaaaaccg gcgacagcag ggcggccgtt gcgatcgtgg
     1681 ctatccaggc ttttctcatg gggctctcca gtagaggggg gtctgtccct gcataaacat
     1741 cggcactggc ggcggtattc cctcatgctg ccgcgggcat ccgtcgcgtc tgcccgggcg
     1801 tatcacgggt attattctga gtgcctcttt ccccctcgaa cccgtgacca gcgagcgatt
     1861 ctacgccgaa ctccccgtca tcatcgactt cggcgcggtg acccgccccg agtgctatgc
     1921 gccgctgccg gacgactggc acgtcgcgct gtgcgacgtg cgcaactcca ctctcgcggt
     1981 ccagaacggc aaatacaaga acgtcaacac ggtcggcgcg gccgccatca cggcggtgct
     2041 gaacgccgcg ggcgatctcg acatcccgtt cagcttcgag ggtgacggct gcgtgctgtg
     2101 cgtgccgccg cggctgctcg aggatacgcg cgccgcgctc gccaagacgc agcaaatcgc
     2161 ccgcgactcc ttcgggctcg atctgcgcat cgccacggtg ccggtgacgc gcatacgcga
     2221 atccggttat gccatccgcg tcgcgcgcat ccgggtctcg gagcattacg tgcaggcggc
     2281 cttcgcgggc ggcggcatcg cccacgccga caggctgatc aaggacccgg cgatgtcgcg
     2341 ttcgtacgtg atcgagcccg gcagcgtcgc gccccgcggc agccacgacg gtctcgaatg
     2401 ccgctggcag gacataccca gccgccacgg cgagaccgtc tgcctgatgg tgcgcgcgct
     2461 cagtgccgac gccggccgcg ccgccgcgct ctacggggaa gtcatcgcca gcgtgcgcga
     2521 tatctatggc ggcgacgaag cctgccatcc ggtcgcgctg cccgcccttt ccctgtcgct
     2581 gtcgggagag cgcctgggca acgaggtggg cgtgcgcgcc gccggccgcg ggacgccggg
     2641 caagtggctc gcgctgatgc gcacgcgctg ggtcgtgctg ctcggctggt tcctgatgaa
     2701 gttcggcatc cgctccgggg acaccgactg gggccgctac aaggagacgc tggtgagcaa
     2761 caccgatgtg cgcaagttcg cggacatcta ccggcaggtg ctcgccggca ccgaagcgca
     2821 acgcaacgcg ctggccgcct ggctggaggg acgttatgcg cgccgcgagc tggtctacgg
     2881 gctacacgtc accgaccgcg cgcacatgac ctgcctggtg ttcaactatg ccggccggca
     2941 cctgcatttc atcgacggcg ccgacggcgg atttttcctc gccgcgcggg cgttcaagga
     3001 acgagccgcg aagctggccg ggcgctaaaa caagaaacga gaaacgagga acgaatcact
     3061 tctttcgcgc cgtcagcggg cgcccgccgc ttccaggatt ctgaccattt ccgcgtaggc
     3121 actgccgcgg gcgcgggcga gcgcaagcgg cgtgcgccgg ttgcggtcgg ccaggttcac
     3181 gctggcgccg gcttccacca gcgccctcag cgtgtcggtg tggcgcgggc cgccgtcgcc
     3241 cagcacgatc gattcgatca gcgcggtcca gcccaggttg ttgacgtggt cgagcggcgc
     3301 gccggcgcgg atcagcgtgc gcacgacctc cgcgtggccg aggtgcgcgg cggcgatcag
     3361 cgcggtgccg tcgtagcggc tggtgacgtt cttcgcgctc gctccgagct ccagcgcgag
     3421 cttgagcgtc ggcacgtcgt tcgcgaccga ggcgatcgtg acgatgtcgt agcggtcgcg
     3481 ctccagcgcg ttgggattgg cgcccgcggc ggcgagcgcg cgcatcgctt cgtggtgttt
     3541 cccgtaggcc gccacgtgca gcggcgtgcg ctggtggctg tcgcgcgcgt ccggcttcat
     3601 gcctttcgcg gtcagcgccc tgatctgcgc cgcgtcaccc tgtgccgcgg cggcgaggag
     3661 gccggtgtag cccgcccgct cggccgggga cggcgcaatt tgcgcttgcg agccgagcgc
     3721 aaagcatgcc gcaagcagca gggtggcgaa ccgcgccggt tcgagcgaac gaacggcgcg
     3781 gccgtcgcgg agacgggtca actgatgaat cgcgggctgg agctcgttcg ctgccgctgt
     3841 cctagcggta cttctcggcg cactggtgaa tctccatgtt ggtggcgagc tcgaggcagc
     3901 tcctggcgtc ctcgttggcc cgcgagcgcc cggcgcttcc cgaagccagc ttgaccgccg
     3961 gctgcgcggg ggccgccgct ggcttggcct ccggttgcac cggggctgcc gcgggttgca
     4021 ccggagccgc ggcaggcttg gccgccggtt gcgccggggc cgctgcgggc ttggcctgcg
     4081 ccaagcccag gaccggcagg gacaacagca ccgcgaatac cgctactgga ccgatgggct
     4141 tcatggttcg gctcctctca aagagaaaca tgatagtggc gaggcggttc agcgccaagc
     4201 gcgggaataa gtcacgtccc ggcttacgcg ccctcggcgg cccccggatt ccactgtccc
     4261 ttgcgcatgc gcgtaatggt gatgctcgag aacacgccgt tctgcgtgaa cggatcgcca
     4321 tccgagaacg ccttggcctt ggcgcggctg tccacgttga cgatgaacag gctgccgatc
     4381 gcctgcgtgc cgtcgtcatt ctgcgtcgcg cccgcgagca ccaggatgcc tttctggctt
     4441 cccaggtagt cgcggtgcgg ctgcagcact ttttcacgga tcgcggcggt attcggcttg
     4501 tcgacgcaag aaatggccca aagcatggtg ttctctcctg gatgaaaatg gttgcagagc
     4561 gagattacca gattccggat tccggaactg cgggcgcgcc cgttccccat tacaatcgcc
     4621 attcgatcat catatcggca gcgggattcc acgaaatggc gggcaaggac cggaaaatcg
     4681 ccgtgctggg ctgcggcgcc atcggcagca gcgtgagcgc cgacctgacc atggcgggtt
     4741 acgacatcac ggtcatcgac cagtggcccg agcaggtgga ggcgctgagg gcggccggcc
     4801 tgcacatcgg gatgaaggac ggggacgtga agacgccccc gatccgggcc ctgcacttgt
     4861 gcgacctcgc gtcggccaac ctggaattcg acatcgtgct gctggcggtg aaatcgtacg
     4921 atcaccgctg gctggcggaa ttcatcaagc cgtacctcaa gaccgacggc gtcgtggtcg
     4981 gcgtcatgaa cggcatgaac gacgactcga tcgcctccat cgtcgggcgc aaccgcaccg
     5041 tgggctgttg catcgagctg tcggcggaaa tcttcacccc cgggcaggtg cagcgcaaca
     5101 ccacgcacca gggcacctgg ttcgcggtgg gcgagctcga cggcttctat accccgcgcg
     5161 tgaaggagat ccagtccatt ctgtcccacg tggcccggtg cgacgtcacc aacaacatct
     5221 acggcgccaa gtggaccaag ctcatcgcca attcgatgac gatggggccg cacgggctgc
     5281 tgggcctgag aaacggcgag gccgcgcacc tgcccggcat ggccgacatc gccgtgaaga
     5341 tcggccgcga gtcgctcgcg gtgggcgtcg cgatgggcta ccgcatcgag ccgatcttcg
     5401 gcctgcgcgc ggacgagttc gcgggctcca gcgacgagaa cctggtgacc gcccgggaca
     5461 cgctgatgca ccacgtcggc ggcaactccc gcaccgcgcc gatccacgac cacatcaagg
     5521 ggcgcaggag cgaaatggat ttcatcaccg gcatcgtcac gcgcaagggc aaggagctcg
     5581 gcatacccac gccgttcaac gacgccgtgc tcgaaatcga ccggatgatc aacaaggccg
     5641 agatcaggat ggatcgctct aatttcgatc tgctgatggg caagctggcc ccgcacatga
     5701 agtgatgggt gacgggaaaa cagctacaag tcacaaggta caaggtgcaa gggttgtacc
     5761 ttgaaccttg agccttgcaa cttgaacctt gatcctgatc aacccatcac ccatcccccg
     5821 catcagcgcg ccaggctgac gttcaccggc gagaacttga cccgctgcac ttccgcgatc
     5881 cggctgccgg ccacgcggcc ttcgacgatt tcgtagccca tcagcgcgaa cacccggttg
     5941 ccgagaaaga tcgggcgggc attgccgtac cagtccacgc aggaggcgcg gcagccgtcg
     6001 ttggcattgg ccgcggcctg cgagtcgagc gcgcccagtt cggagaggcg cagcccctgg
     6061 tttcgcagga acagcaccgc ggccgagttg tgccgcagct ggcgcgcggc ggactgcccg
     6121 ccgccgatga ccggcagccc gaccatgccg gcgttgtcgc tgtccggctt gtagaaaaag
     6181 ccgtggctgc gcgtctcgcc ctgcgccgcg tccttgcgcg tatagcggtc ctgcgccgag
     6241 gcggtcgccg cgagccgcag gctggtgaaa tgcagatcgc gcccgtccga gcccacgatc
     6301 accgcgttgg cgccgagcgc ctcgatgcgg tccacgccgt gcgcgagcgg gatcgcctgc
     6361 gcgccggccc cggacgcgta gcgcaccgcg taaacctgcg agtgcagcat gcgctgcgcg
     6421 cggttccagc cggtgccggt gccgtagagc aggtacgggc cgacgtagcg gttctgcacc
     6481 gcgtggccgc cgggccgggg cagcggcttg taggcttccg ccggcgccgc gtcgcgcccg
     6541 tcggagaagc tgtcgagaga cacccgcagc agcgcgaaat cgccggcgtt ggcttccgcc
     6601 gcccacatgc cctcgccgcg gccgtcggcg cgcaccagca cgttgagatg gttgtcgttg
     6661 ccctcgaaga aggagaactg gtcgatcggg ctgcccgccg tcttcagcgc ggtcggcgcc
     6721 gcgccgtcga gcgggatgcg gaatacggcc gaccctggcg cagacgcggc gcggttgcgg
     6781 cggccctgcg tcgtccagac gtagaccgag ccgcccgaga cgtagaacac gcgccccggc
     6841 gagccgagca ccgcggtgct ctcgcaatcc atctcggcct tcgcgaggtc gcacaccgtg
     6901 accgtgtgca gcgcgacgcc ctggtggggg tcgagcggct catcggtgcg gtagatgcgg
     6961 gtcgccggcg cgatcggctt gaactcggcg gcggtggccc cggggcgcca gcggcgcatc
     7021 gcggggaacg agccccaggg ctcgccgccc cagggattga gatgcatcgg cgtatagaac
     7081 accagcttgt cgccgatcag ccggctcgcg taattgcgcg aggagtagta atcgttcgat
     7141 cgcaggtggt aggtggaccg gtagcgcagg cggcccgcgt ccgagatctc gaacaggccg
     7201 atttccgtgc cgccgcgctg gtagctgtag ccgatcacgg cgatggtgtt gccggagatc
     7261 agcatctcgt cgtaccacgc gccgcccggg ttgatcccgc tgccgtaggc gtcggcaacc
     7321 gacgccggcg tcagttcctc gccgaccttg acggtgaaga ggcgcccgcg ccgcaggatc
     7381 acgaggtggt cgccgtggcg cttgacgatg ccgccttcgt ccacgcccgc gtgctgcacg
     7441 ttggtgatgg aatccgcgga ggcttcagcg gccgccttcg ccgaagccgg cgcggcggac
     7501 atgagggcgc ccatgccgga tgcatcctgg taggcctgcg tcgagcggcg ttccgcgtcg
     7561 cgcttgcggg cctcggcggc ccagcgcttg aacaggtccg agatttcctg ctcgctggcg
     7621 aacgccgtca gggttttccc gggtgcggcg tgcgcgcccg tagttacgag catgatggcg
     7681 gcgaaggcgg atgcgacggc cacccgcagc aatttttgat tcggcatggt tgctctcctg
     7741 ggggttgttg ttcatcagcc agccggatca aggagttacg actgcggctt ccccgatcgc
     7801 gctgggctac gggggctata aacggcaag
//
LOCUS       DBNGSG010000236         1159 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_585219, whole genome shotgun sequence.
ACCESSION   DBNGSG010000236 DBNGSG010000000
VERSION     DBNGSG010000236.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_585219"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="AC158Q_06235"
     CDS             <1..279
                     /locus_tag="AC158Q_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3339408.1"
                     /translation="LGYADAGERTLKVELAMEVNRILAERALSQDRVAQLLGIRQPHV
                     SDLVRYRLNRFSVERLMDFLARLGKDVEIRIASRRTRRRSAVHVRLVA"
     gene            complement(634..852)
                     /locus_tag="AC158Q_06240"
     CDS             complement(634..852)
                     /locus_tag="AC158Q_06240"
                     /inference="COORDINATES: protein motif:HMM:NF016167.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HIR3339409.1"
                     /translation="MLDWSKCPAVESDSGKLSGAWRFRGTRVPVSALFENLEGGATVE
                     EFLRWFPGVSRAQVDAVLEHAARSAQTA"
     gene            complement(896..>1159)
                     /locus_tag="AC158Q_06245"
     CDS             complement(896..>1159)
                     /locus_tag="AC158Q_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3339410.1"
                     /translation="FMDYALPRAEDFPAPFPWQSHPVPATTNPLGAKGCGEAGCAGSM
                     TSVMNAVVDALSELGIRHFDMPASAPRVWAAIQQAKNRGQSPK"
BASE COUNT          187 a          405 c          372 g          195 t
ORIGIN      
        1 ctcggctacg cggacgccgg ggagcgcacg ctcaaggtcg agctcgccat ggaggtgaac
       61 cgcatcctgg ccgagcgcgc tctctctcag gaccgcgttg cccagctact cggcatccgg
      121 cagccgcacg tctccgacct ggtgcgctat cggctcaacc gcttttccgt cgagcgcctc
      181 atggacttcc tcgcccggct cggcaaggac gtggagatcc gcattgcgtc gcggcgaacc
      241 cgccgccgct ccgccgtgca cgtccgactt gtggcgtgac gggaatggga gtctgagctc
      301 gatcggtcag agctcaattt cgtgatactg acccggccga agagcgctca ccgcagccgc
      361 aatctcttca cccctaccgc gaagccgcgg ccagcttgtg gtggggagga cgacgatggc
      421 cagctggcga ccggcgagat tttgctggct ccgcaggttg cggtcggtcg taatcagcac
      481 gtcgaaacgg ccttcggccc gagataacag ttcgccgttg cttatcgtgg atcagccaag
      541 ctcgaaggct gtcgtaacgc tgtgcccagg aagcagctcg cgtagcggtg cggggacgcc
      601 ctggtcgaaa agaacgcgca ccccgcgcgg ctgtcaggcc gtctgcgcgc tgcgtgcggc
      661 gtgctccagc accgcgtcga cctgtgcgcg cgagacaccg gggaaccagc gaagaaactc
      721 ctctaccgtg gctccgccct cgaggttctc aaagagcgcg cttaccggca cgcgggtgcc
      781 gcggaaccgc caggccccgc tcagtttccc ggaatcggat tcgaccgccg gacactttga
      841 ccagtcgagc atcgaccgat tctactcccg gcatagcgcc ggccctcacc ccgaattatt
      901 tcgggctctg accccgattt ttcgcctgct ggatcgccgc ccagacccgc ggcgcactcg
      961 ccggcatgtc gaagtgcctg atgccgagct ccgagagcgc gtccaccact gcgttcatca
     1021 cggaagtcat cgagcccgcg cagcccgcct cgccgcagcc cttcgcgccg agcgggttgg
     1081 tggttgcagg caccgggtgg ctctgccacg ggaagggcgc ggggaagtcc tcggcgcgcg
     1141 gcagcgcgta gtccatgaa
//
LOCUS       DBNGSG010000237         7105 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3641053, whole genome shotgun sequence.
ACCESSION   DBNGSG010000237 DBNGSG010000000
VERSION     DBNGSG010000237.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7105)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7105)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7105
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3641053"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="AC158Q_06250"
     CDS             complement(<1..568)
                     /locus_tag="AC158Q_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051258 - protein polymerization [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIR3339411.1"
                     /translation="MFEIVDAQAQEAVIKVVGVGGCGGNAVDHMIEKGVQGVEFITAN
                     TDAQALKRNQSKIQLQLGTGITKGLGAGANPEVGRQSALEDRERVAEAIAGADMLFLT
                     AGMGGGTGTGAAPVVAELAKELGILTVAVVTKPFAFEGKRQRIAQEGIEALSPHVDSL
                     IVVPNDKLMEVLGNQVTLDEAFKAANDVL"
     gene            complement(604..1842)
                     /gene="ftsA"
                     /locus_tag="AC158Q_06255"
     CDS             complement(604..1842)
                     /gene="ftsA"
                     /locus_tag="AC158Q_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014898179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HIR3339412.1"
                     /translation="MSKARDHKQLIIALDIGTSKIAAIVAQIRPDGGFEIIGMGNHPS
                     RGLKKGVVVNIETTVNAIQRALEEAELMADCKIREVYTGIAGSHIRSFNSQGMVAVKD
                     KEVAQPDIERVVDTARAVQIPNDQQILHVLNQEFIIDGQEDVREPLGMSGVRLEVKVH
                     IVTGAVSAAQNIIKCVRRCGLEVRDLILQPLASASAVISEDEKDLGVALMDIGGGTTD
                     LVVYTHGAIRHTAVIPIAGDQITNDIAMALRTPTKDAEDIKERYGCALSQLADPQDMV
                     EVPGLGEREPRQLSRKTLAEVIEPRVEELYSLVQTELRRSGYEELLSSGVVITGGSSV
                     MQGMVDLGEEIFHMPVRVGYPGYNGALAETVRHPRYSTGVGLLLAGLQQHRSRELARM
                     NVSPVQHALDRMKSWFTGNF"
     gene            complement(1858..2598)
                     /locus_tag="AC158Q_06260"
     CDS             complement(1858..2598)
                     /locus_tag="AC158Q_06260"
                     /inference="COORDINATES: protein motif:HMM:NF015739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIR3339413.1"
                     /translation="MWDKPALMNSLANALFAAAALLVLAAVAIEVARLPAFSLREVRV
                     VGEVKHVARDEVEDVVRRELRGNFFTIDLPATRAAFERLPWVRNVNVRRQWPARLDIA
                     LEEHVPFARWGAGSLVNTHGEVFTAAHDGELPIFAGPDGTAREIAIQYRYFQRSLGTI
                     GETPVEVQVTARRAWQVKLASGMTLALGREAVEARLERFVAAHQRTLGRLGRRIDYVD
                     LRYANGFAVRIPELRDGKPATRRGRRTG"
     gene            complement(2588..3502)
                     /locus_tag="AC158Q_06265"
     CDS             complement(2588..3502)
                     /locus_tag="AC158Q_06265"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HIR3339414.1"
                     /translation="MTVNFGKVAVLMGGRSAEREISLKSGTMVLGALAKSGVDAHAFD
                     PRDSGLEALKSQRYDRAFIALHGRFGEDGTVQGALEYLGIPYTGSGVMASALAMDKWR
                     AKLLWQAAGLPTPPCELMTARTDSVDVAKRLGLPLMMKPVREGSSIGMTKVTSVEKIA
                     PAYELAARYDDVVIAERFIQGIELTAAILGDEPLPLIRLETPREFYDYEAKYFANDTR
                     YICPCGLPGGQERSVQRTALEAFRVLGCTGGGRVDLMLDRDGSPYLLEVNTIPGMTDH
                     SLVPMAARARGIAFEELCVRILETAHVG"
     gene            complement(3541..4509)
                     /gene="murB"
                     /locus_tag="AC158Q_06270"
     CDS             complement(3541..4509)
                     /gene="murB"
                     /locus_tag="AC158Q_06270"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HIR3339415.1"
                     /translation="MVEANNLTAAGLRGELRLREPMARHTSWRAGGAAERAYFPADLD
                     DLAAFLRALPAEEMVHMTGLGSNLLVRDGGLRGTVIFTHWALRGVRHESPGGILAEAG
                     VASPRVARFAARHDLAGAEFLAGIPGTVGGALAMNAGCYGGETWDIVAGVTTVDRSGS
                     LHKRAREDYEVAYRHVRLKAEGAKQKAEGVVPHPSSLIPHPPEEWFVAATFRLQHGPA
                     VESRRKITELLKRRIATQPLGEPNAGSVFRNPAGDYAARLIEACGLKGRVIGGAQISE
                     KHANFIVNLGRARAADIEALIQLAERAVKERFGIALEREVRIVGDA"
     gene            complement(4512..5933)
                     /gene="murC"
                     /locus_tag="AC158Q_06275"
     CDS             complement(4512..5933)
                     /gene="murC"
                     /locus_tag="AC158Q_06275"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIR3339416.1"
                     /translation="MRHKVKQVHFVGIGGSGMSGIAEVLLTLRYKVTGSDLAENASTR
                     RLRQLGAKIHIGHDEAHIAGADAVVVSTAVKDENPEVAAARARRIPLVPRALMLAELM
                     RLKQGIAVAGTHGKTTTTSLVASVLAEGGLDPTFVIGGRLEAAGSSARLGAGEFIVVE
                     ADESDASFLYLQPTLAVVTNIDADHMETYDQSLDKLRQAFVDFLQRLPFYGVAVLCAD
                     DPNVRAIQPQLTKAVTTYGLAADANVRALDVTASGTQMRFRAVRAGADRAPAAELAVT
                     LNLPGVHNVRNALAAIAVGIEVGVPEAKIVKALAEFKGVGRRFQNHGKVRLAGGGSFT
                     LVDDYGHHPAEMAATLAAARGAFPKRRILLAFQPHRYTRTRDCFEDFVSVLSTADALV
                     LTEVYPAGEAPIVAADGRALARAVRVQGKVEPVFVEKVADLPAAILAAARDGDVVLTM
                     GAGSIGNVAGILASRVRSVNGAA"
     gene            complement(5995..7098)
                     /gene="murG"
                     /locus_tag="AC158Q_06280"
     CDS             complement(5995..7098)
                     /gene="murG"
                     /locus_tag="AC158Q_06280"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012763013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HIR3339417.1"
                     /translation="MSDERKTSGVSRTIMIMAGGTGGHIFPALAVAEHLRAQGWKVVW
                     IGVRGGMEERLIPPRGYAMSWVRFSGVRGRGPVTFALLPLSLLIAFWQTARAIFAHRP
                     DVVLGMGGYVSFPGGMMASLFNKPLAIHEQNSIAGLANRVLAKLADRVLTTFPSAFGE
                     ATAVIWTGNPVREEIVAMAPPENRYAARAGQLRLLVVGGSLGAQVLNTVMPEALALIS
                     PEARPRVTHQAGAAHHAAVKDRYQACAVDATVLPFIDDMAGRYAETDLIVCRAGATTV
                     AEIAAAGIASVLVPYPHSVDDHQTINARFLADRGAALLIPQSELTAQGLADVLSKFTR
                     ERLLAMAQSARAAGRPGATRAVAQACVELAGAA"
BASE COUNT         1067 a         2545 c         2287 g         1206 t
ORIGIN      
        1 gaagcacgtc attggcggcc ttgaacgcct catccagcgt cacctggttg ccgagcactt
       61 ccatcagttt gtcgttgggc accacgatca gcgagtcgac atggggcgac agcgcctcga
      121 tgccttcctg tgcgatgcgc tggcgcttgc cctcgaacgc gaacggtttg gttaccaccg
      181 cgaccgtcag tatgccgagt tccttggcga gttcggccac gaccggcgca gcgccggtgc
      241 cggtgccgcc gcccatgcca gccgtcagga acagcatgtc ggcgccggcg atggcctcgg
      301 cgacgcgctc gcggtcctcc agcgccgact ggcgcccgac ctcggggttg gcccccgcgc
      361 cgagcccctt ggtgatgccg gtgccgagct gcagctggat cttcgactgg ttgcgcttca
      421 gcgcctgcgc gtccgtgttg gcggtgatga actccacccc ctgcacgccc ttctcgatca
      481 tgtggtcgac cgcgttgccg ccgcagccgc ccacgccgac caccttgatt acggcttcct
      541 gtgcttgtgc gtccactatc tcaaacatgg ttgtctcctt tttacgtcaa ccgatgcaaa
      601 aaactagaaa tttcccgtga accatgactt catccggtcc agcgcgtgct ggaccgggct
      661 tacattcatg cgcgcgagct cgcgcgaacg gtgctgctgc aggcccgcca gcagcaggcc
      721 gacaccggtc gagtaacgcg gatggcggac ggtttccgcc agagcgccgt tgtagcccgg
      781 gtagcccacc cgcaccggca tgtgaaatat ttcctcgccc aggtccacca tgccctgcat
      841 cacgctcgag ccgccggtga tcaccacgcc cgaggaaagc agttcctcgt agccgctcct
      901 gcgcagttcc gtctgcacca gggaatagag ttcctccacg cgcggctcga tcacctcggc
      961 gagcgtcttg cgcgaaagct ggcgcggttc gcgctcgccc agtcccggca cctccaccat
     1021 gtcctgcgga tcggcgagct gcgagagcgc gcagccgtaa cgttccttga tgtcctcggc
     1081 atccttggtt ggcgtccgca gcgccatggc gatgtcgttt gtaatctggt cgccggcgat
     1141 cgggatcacc gcggtgtggc ggatcgcgcc gtgggtgtac acgacgagat cggtggtgcc
     1201 gccgccgatg tccatcagcg cgacgcccag gtccttttcg tcctccgaga tcaccgcgga
     1261 ggccgaggcg agcggctgca ggatgagatc gcgcacctcg agcccgcagc ggcgcacgca
     1321 cttgatgatg ttctgggccg cggatactgc cccggttacg atgtgcacct tgacttcgag
     1381 acgcacgccg ctcatgccga ggggctcgcg cacgtcctcc tgcccatcga tgatgaattc
     1441 ctggttgagc acgtgcagga tctgctggtc attcgggatc tgcacggcgc gcgccgtgtc
     1501 gacgacgcgc tcgatatcgg gctgcgcgac ttccttgtcc ttcaccgcga ccatgccctg
     1561 agaattgaag ctgcggatgt ggctgccggc gatgccggta tagacctcgc ggatcttgca
     1621 gtcggccatc aactcggctt cctccagcgc ccgctgtatc gcgttcaccg tggtctcgat
     1681 gttgaccacc acgcctttct tcaggccccg cgacgggtga ttgcccatgc cgatgatctc
     1741 gaagccgcca tccggccgga tctgcgcgac gatcgcggcg atcttggacg tgccgatgtc
     1801 gagggcaatg atcagttgct tgtggtctct ggccttgctc attcgcgctg ttccgtccta
     1861 tcccgtccgc cgtccgcgcc gggtcgccgg cttgccgtcg cgcaattccg gtatgcgcac
     1921 ggcgaagccg ttggcgtagc gcaggtccac gtaatcgatc cgccgcccaa ggcgcccgag
     1981 ggtgcgttga tgcgccgcca cgaagcgttc gaggcgcgcc tcgacggctt cgcgcccgag
     2041 cgcgagcgtc atgccgctcg cgagcttcac ctgccaggcg cgccgcgccg tgacctgaac
     2101 ctccaccggc gtctcgccga tcgtgcccag gctgcgctgg aagtagcggt actggatggc
     2161 gatctcgcgg gcggtgccgt ccggaccggc gaatatcggc agctcgccgt cgtgagcggc
     2221 ggtgaagacc tcgccatgcg tgttgacgag cgagccggcg ccccagcgcg cgaacggaac
     2281 gtgttcctcc agcgcgatat ccagccgggc gggccactgg cgccgcacat tcacgttgcg
     2341 cacccaaggc agccgctcga acgcggcgcg cgtggcggga agatcgatcg tgaagaagtt
     2401 tcctctgagc tcgcgccgca cgacgtcctc gacctcgtcg cgggcgacgt gtttgacttc
     2461 accgacgacc cgcacctcgc gcagggagaa cgccgggagc cgcgccacct cgatcgcgac
     2521 ggcagcaagc acgagcagtg ccgcggcggc gaagagcgca ttggcaagcg agttcatgag
     2581 cgccggctta tcccacatgg gccgtctcca gtatccgcac gcacagctcc tcaaaagcga
     2641 tgccgcgggc acgggcggcc atcggcacca ggctgtgatc agtcatgccg gggatggtgt
     2701 tgacctccag caggtacggg ctgccgtcgc ggtcgagcat caggtccacg cgccccccgc
     2761 cggtgcagcc gagcacgcga aaggcctcca gcgccgtgcg ctggaccgac cgctcctggc
     2821 cgccaggcaa cccgcacggg cagatgtagc gggtgtcgtt cgcgaagtac ttggcctcgt
     2881 agtcgtagaa ctcgcgcggc gtctcgagcc ggatcagcgg cagcggctca tcgccgagga
     2941 tggcggcggt gagctcgatg ccctggatga agcgctccgc gatcaccacg tcgtcgtatc
     3001 gggcagccag ctcgtaggcc ggggcgattt tctcgaccga ggtcaccttg gtcatgccga
     3061 tcgaggaacc ctcgcgcacc ggcttcatca tcaacgggag ccccagccgc ttcgcgacgt
     3121 ccacggaatc ggtgcgcgcc gtcatgagct cgcaaggcgg cgtgggcagc cccgccgcct
     3181 gccacaggag cttggcgcgc cacttgtcca tcgccagcgc gctcgccatc acgccgctgc
     3241 cggtatacgg gatgccgagg tactcgagcg cgccctgcac cgtgccgtcc tcgccgaagc
     3301 gaccgtgcag cgcgatgaac gcgcggtcgt agcgctgcga cttgagcgcc tcgaggccgc
     3361 tgtcgcgcgg gtcgaaggca tgcgcgtcga caccgctctt cgcgagcgcg ccgagcacca
     3421 tcgtcccgct cttgagggaa atctcccttt cggcggacct accacccatg agcaccgcca
     3481 cttttccgaa attgaccgtc actcgtcact cgtcacttgt cactcgtcgt tcgtctctcg
     3541 ttacgcatca cccacaatgc gcacttcccg ctccagcgcg attccgaacc gctccttcac
     3601 cgcccgctcc gcgagctgga tcagggcctc gatatcggcg gcacgcgcgc gcccgaggtt
     3661 gacgatgaaa ttcgcgtgct tctccgagat ctgcgcgccg ccgatcacgc ggcccttcag
     3721 cccgcacgcc tcgatcagcc gcgccgcgta atcgcctgcg ggattgcgga acaccgagcc
     3781 ggcgttgggc tctcccaggg gttgggtcgc gatgcggcgc ttgagcaatt cggtaatctt
     3841 cctgcgggat tccactgcgg gcccgtgctg gaggcggaac gttgccgcga cgaaccattc
     3901 ctcgggagga tgagggatga gggatgaggg atgaggaacc accccttccg ccttctgctt
     3961 cgcgccttcc gccttcaagc gcacgtgccg gtaggcaact tcgtagtcct cgcgcgcgcg
     4021 tttatgcaac gaaccgcttc gatcgaccgt cgtcacgcct gcgacgatgt cccaggtctc
     4081 cccgccgtag cagcccgcgt tcatcgccag cgcgccgccc acggtgccgg ggatgccggc
     4141 gaggaactcc gcgccggcaa gatcatggcg cgcggcaaaa cgcgccaccc tggggctggc
     4201 gacgccggct tcggccagga tgccgcccgg ggattcgtgg cggacgccgc gcagtgccca
     4261 atgggtgaag atcacggtgc cgcgcagtcc gccatcgcgc accagcaggt tgctgcccag
     4321 ccccgtcatg tgcaccatct cctccgcggg cagcgcgcgc aggaacgcgg cgagatcgtc
     4381 gaggtcggcg ggaaagtagg cgcgttcggc cgcgccaccg gcgcgccagc tcgtatgccg
     4441 cgccatcggc tcgcgcaacc gcagctcgcc tcgcagcccg gctgccgtca gattgttcgc
     4501 ttccaccatg ttcatgctgc gccgttcacg cttctcaccc ggctcgcgag gattcccgcg
     4561 acgttgccga tcgagcccgc gcccatcgtg agcaccacgt cgccatcacg cgccgcggcg
     4621 aggatggccg ccggcaggtc ggcgaccttt tccacgaaca ccggttcgac ttttccctgc
     4681 acccgcaccg cgcgggcgag cgcgcggccg tccgccgcca cgatcggcgc ctcgccggcc
     4741 gggtagactt ccgtcaatac cagcgcgtcc gccgtggaca gcaccgacac gaaatcctcg
     4801 aagcagtcgc gcgtgcgcgt gtagcggtgc ggctggaacg cgagcaggat tcttcgttta
     4861 gggaacgcgc cgcgcgcggc ggccagcgtc gccgccattt ccgccgggtg atggccgtag
     4921 tcgtccacca gcgtgaagct gcccccgccg gccaggcgca ccttgccgtg gttctggaac
     4981 cggcgcccga cgcccttgaa ctcggcgagc gccttcacga tcttcgcctc cggcaccccc
     5041 acctcgatgc ccaccgcgat cgccgcgagc gcgttgcgca cgttgtggac accgggaaga
     5101 ttcagggtga ccgccagctc ggcggccggc gcgcggtccg cgcccgcgcg caccgcccgg
     5161 aatcgcatct gcgtgccgct cgccgtgacg tcgagcgcgc gcacgttcgc gtcggcggca
     5221 agcccgtagg tcgtcaccgc cttggtgagt tgcggctgga tcgcgcgcac gttggggtcg
     5281 tcggcgcaca gcaccgccac cccgtagaac ggcagccgct gcaggaaatc cacgaacgcc
     5341 tggcgcagct tgtcgagcga ctggtcgtag gtttccatgt ggtcggcgtc gatgttggtg
     5401 accaccgcca gcgtcggctg caggtacagg aaagaggcgt cggattcgtc ggcctcgacg
     5461 acgatgaact cgccggcgcc gagccgcgcg ctggagcccg cggcctcgag ccggccgccg
     5521 atcacgaacg tcggatcgag gccgccctcg gccagcacac tcgccaccag gctcgtcgtc
     5581 gtggtcttgc cgtgcgttcc cgccaccgcg atgccctgct tcaggcgcat cagctcggcg
     5641 agcatcagcg cccggggcac cagcgggatg cggcgcgcgc gcgccgccgc gacttcgggg
     5701 ttctcgtcct tcaccgcggt cgagaccacc accgcgtccg ccccggcgat gtgcgcctcg
     5761 tcgtgcccga tatggatctt ggcgccgagc tgccgcagcc ggcgggtcga agcattctcg
     5821 gcgagatcgg agccggtcac cttgtagcgc agcgtcaaca gcacctcggc gatgccgctc
     5881 atgccggatc cgcctatgcc aacgaaatgc acttgcttga ccttgtgcct catcggatgt
     5941 gaccgctgtc gcagcccctt ttgccttttg ccttttgcct tttgccttgt ctcttcatgc
     6001 tgcccccgcg agttccacgc acgcctgcgc gaccgcccgt gtcgcccccg gcctgccggc
     6061 ggcgcgcgcc gattgcgcca tggcgagcag ccgctcgcgc gtgaatttgg agagcacatc
     6121 ggccagcccc tgcgccgtca gctcgctttg cggaatcagc agcgcggcgc cgcggtcggc
     6181 gagaaagcgc gcgttgatcg tctggtgatc gtccaccgag tgcggatagg gcaccagcac
     6241 cgaggcaatg ccggccgcgg caatctcggc cacggtggtc gcgccggcgc ggcagacgat
     6301 caggtccgtc tccgcgtagc ggcccgccat atcgtcgatg aagggaagga ccgtggcgtc
     6361 gacggcgcat gcctggtagc ggtccttcac cgcagcgtgg tgcgccgctc ccgcctggtg
     6421 cgtgacgcgc gggcgcgctt ccggcgagat cagcgcgagc gcttcgggca tgacggtatt
     6481 gagcacctgc gcgcccaggc tcccgcccac taccagcagc cgcaactgcc cggcgcgcgc
     6541 cgcgtagcgg ttctccggcg gcgccatggc gacgatctcc tcgcgcaccg ggttgccggt
     6601 ccagatcacg gcggttgctt cgccgaaagc gctggggaaa gtagtgagca cgcggtcggc
     6661 gagtttcgcc agaacgcggt tcgccaggcc cgcgatcgag ttctgctcgt ggatcgccag
     6721 cggcttgttg aacagcgagg ccatcatgcc gcccggaaac gagacgtagc cgcccatgcc
     6781 gagcaccacg tcgggacggt gcgcgaagat cgcgcgcgcg gtctgccaga acgcgatcag
     6841 caggctcagc ggcaataacg cgaaggtgac ggggccgcgg ccgcgcacgc cggagaaacg
     6901 cacccacgac atggcgtaac cgcgcggcgg aatcagccgc tcttccatgc cgccgcgcac
     6961 cccgatccat accactttcc agccctgcgc gcgcaggtgc tccgccaccg ccagcgccgg
     7021 gaagatgtgc cctcccgtgc cacccgccat gatcatgatc gtcctcgaaa cgccggaggt
     7081 tttccgctca tcactcatca ctctt
//
LOCUS       DBNGSG010000238         4934 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3641114, whole genome shotgun sequence.
ACCESSION   DBNGSG010000238 DBNGSG010000000
VERSION     DBNGSG010000238.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4934)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4934)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4934
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3641114"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..176)
                     /locus_tag="AC158Q_06285"
     tRNA            complement(90..176)
                     /locus_tag="AC158Q_06285"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(140..142),aa:Leu,seq:cag)
     gene            219..2468
                     /gene="rnr"
                     /locus_tag="AC158Q_06290"
     CDS             219..2468
                     /gene="rnr"
                     /locus_tag="AC158Q_06290"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006476122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HIR3339418.1"
                     /translation="MNQRKKRTSGTSRQRSRDPHHEREASRYAHPVSSREFILDTLTE
                     QGVPVTEEELGRLLKIPPQDRDAFAKRLSAMQRDGEIMRNRRDAICVVAKLDLVRGRV
                     QGHADGFGFLVRDEGGPDLFLGPKEMRGVMHGDRAMARISGTDRRGRPEGQIVEVLER
                     GHQRLVGRLRSEHGILFVAAEDKRISHEFLVPPDAAGGAKPGQVVTVEIVAQPSRHAQ
                     PVARVVEVLGNYADPGMEIEIALRKHTLPHAFSQAVEHLCAGIPDTVTAEDQAGRTDL
                     TQLPLVTIDGETAKDFDDAVYCEPLLLSPPYQGGDGKRGVSPPSQGGEDRGGWRLIVA
                     IADVSHYVKHGDALDREAASRGNSVYFPRRVIPMLPEKLSNGLCSLNPKVPRLAVVCD
                     MRVGADGDIASYRFYPAVFRSHARLTYTEVAAMLGDENGEAGRRYRALLPHIQNLHRL
                     YKVLVKARERRGAIDFETIETEMIFDAHGKIERIVPVKRNDAHRVIEECMLAANVCAS
                     DFLHGHDHPMLYRIHEGPTPEKLTALREFLKGFGLQLSGGDDPHARDYAKLLAKVRDR
                     PDAQLIQTVMLRSLKQAVYSPDNLGHFGLAYESYTHFTSPIRRYPDLLVHRAIKAVLK
                     RKHYDPGNWHELGAHCSMTERRADEATRDVVSWLKCYYMKDRVGDVFEGSVSGVTAFG
                     AFVALDGVYVEGLLHVSELGSDYFHYDAGKHHLMGERTRKRYRLGDRVRVKVVRVDLD
                     TSRIDFVLG"
     gene            2503..3867
                     /locus_tag="AC158Q_06295"
     CDS             2503..3867
                     /locus_tag="AC158Q_06295"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HIR3339419.1"
                     /translation="MTIPIWEYRSMIPGAAWPAVPGQEGANLLALQFQLEQTQWLSPE
                     KLGELQFRQLDSLVRHAYGTVPYYRERWRGLYDPGERLTSEGYSRLPLLTRRELQANF
                     DALKSTSIPAAHGLVTESRTSGSTGTPVRVLKTGFVDLLWQAILLREHLWFARDLRGK
                     LASIRHGAAAGEADGWGPATTAIVGTGRSATLPIKSDVDAQLQWLESQRPDYLLTYPS
                     NLAELARRSLARGIRLPALREARTFGEMLPPETREFCRRAWGVPVTDTYSTQEVGYIA
                     LQCPLHEHYHVQSECVLMEALNDEGLACAPGATGRVVITSLHNFAMPLVRYDIGDYAE
                     VGMPCDCRRGLPVLRRVAGRVRNMLVLANGARYWPGLNLGSLAGGAPVLQHQLVQKSF
                     DTIEVRLVTARPLTLEEEQHMRQHLLARLPVPFEINFNYCDAISRGAGGKFEECISEM
                     AATG"
     gene            3999..4742
                     /gene="rlmB"
                     /locus_tag="AC158Q_06300"
     CDS             3999..4742
                     /gene="rlmB"
                     /locus_tag="AC158Q_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004198391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HIR3339420.1"
                     /translation="MAESQLIYGFHAVTSRLRQNAASVKEIYLDQSRNDRRAKDLAQL
                     AAQRGARVMLVDGARLDGMTQHARHQGIVARVEPAALARDLDEVLDGLKEPALLLILD
                     GITDPHNLGACLRVADAMGVHAVVAPKDRAAGITPTVEKVASGAAETVPFIAVTNLAR
                     TMRELKEREIWLIGADQAAERDLYSIKLDVPLAWVLGAEGEGMRRLTRENCDELARIP
                     MLGAVESLNVSVSAGVCLAEARRQRAFRK"
BASE COUNT          896 a         1533 c         1670 g          835 t
ORIGIN      
        1 gccgagtatc gagtgctgag ttctgggtgc aatatgggtt gcgtcgtgcc ttcacagcac
       61 tcggcacatt gcactcagcg cttgggaagt ggtgcccagg aagggattcg aacccccaca
      121 ccattgctgg cgccaggacc tgaacctggt gcgtctacca attccgccac ctgggcttgc
      181 gcgggcggaa ttctagccga acaacccact tttttcaaat gaatcaacgc aaaaaacgca
      241 cttccggcac gtcccggcaa cgttcccgtg atccccatca cgagcgcgag gcgagccgct
      301 acgcgcaccc ggtgtcgagc cgggagttca tcctggacac gctgacggag cagggcgtcc
      361 ccgtcaccga agaagagctc gggcgcctgc tcaagatacc gccgcaggac cgcgacgcgt
      421 tcgcaaagcg cctctccgcc atgcagcgcg atggcgagat catgcgcaac cgccgcgacg
      481 cgatctgcgt ggtggccaag ctcgatctcg tgcgcgggcg cgtgcaggga cacgccgacg
      541 ggttcggctt cctcgtgcgc gacgaaggcg ggccggatct cttcctcggc ccgaaggaaa
      601 tgcgcggggt catgcacggc gaccgcgcca tggcgcgtat ctccggcacg gaccgccgcg
      661 gccggcccga gggccagatc gtggaggtgc tggagcgcgg ccaccagcgc ctggtgggcc
      721 ggctacgcag cgagcacggg atacttttcg tcgcggccga ggacaagcgc atcagccacg
      781 aattcctggt gccgccggac gcggcgggcg gcgcgaagcc gggccaggtg gtgacggtgg
      841 aaatcgtcgc gcagccctcc cggcacgcgc agccggtggc gcgcgtcgtg gaagtgctcg
      901 gcaattacgc cgatcccggc atggaaatcg agatcgcgct gcgaaagcac acgctgccgc
      961 acgcgttctc gcaagccgtc gagcatctct gcgcggggat acccgacacc gtcaccgcag
     1021 aagaccaggc gggacgcacc gatctcacgc agctgccgct ggtgactatc gacggcgaga
     1081 cggcgaagga tttcgatgac gccgtttatt gcgaacccct cctcttatcc cccccttatc
     1141 aaggggggga cggaaagagg ggggtctccc ctccttccca agggggggag gaccgggggg
     1201 gttggcggct gatcgttgcc atcgccgacg tcagccatta cgtgaaacac ggcgatgcgc
     1261 tcgaccgcga ggcggcgagc cgcggcaact cggtctattt tccgcgccgc gtgatcccga
     1321 tgctgccgga gaagctctcc aacggcctgt gttcgctcaa cccgaaagtg ccgcggctcg
     1381 cggtggtgtg cgatatgcgg gtcggcgcgg acggcgatat cgcgtcctac cggttctacc
     1441 cggcggtgtt ccgttcgcac gcgcggctga cctacaccga ggtggcggcg atgctgggcg
     1501 acgagaacgg cgaggcgggc cgccgttacc gcgcgctgct gccgcatatc cagaatctcc
     1561 accgcctcta caaggtgctg gtgaaggcgc gcgagcggcg cggggcgatc gacttcgaga
     1621 ccatcgaaac cgagatgatc ttcgatgcgc acggcaagat cgaacgcatc gtcccggtca
     1681 agcgcaacga cgcgcaccgc gtgatcgagg agtgcatgct cgccgccaac gtgtgcgcgt
     1741 ccgacttcct gcacgggcac gaccatccga tgctctaccg catccacgag ggcccgacgc
     1801 ccgagaagct caccgcgctg cgggaatttc tcaagggttt cggcctgcaa ttgagcggcg
     1861 gggacgaccc ccatgcacgc gactacgcga agctgctcgc caaggtgcgg gaccggccgg
     1921 acgcgcagtt gatccagacg gtcatgctgc gctcgctcaa gcaggcggtt tacagccccg
     1981 acaatctcgg ccacttcggg ctcgcctacg agtcctacac ccatttcacc tcgccgatcc
     2041 gtcgctatcc cgacctgctg gtgcaccgcg cgatcaaggc ggtgctgaag cgcaagcact
     2101 atgacccggg caactggcac gagctgggcg cgcactgctc gatgaccgag cggcgggcgg
     2161 acgaggccac ccgcgacgtc gtgtcctggc tgaagtgcta ttacatgaag gaccgggtcg
     2221 gcgacgtgtt cgagggcagc gtgagcggcg tgacggcctt cggcgccttc gtcgcgctcg
     2281 acggcgttta cgtggaaggg ttgctgcacg tgtccgagct cggcagcgac tacttccatt
     2341 acgatgcggg caagcaccac ctgatgggcg agcgcacccg caagcgctac cggctcggcg
     2401 accgcgtgcg cgtgaaggtg gtgcgtgtgg acctcgacac cagccggatc gatttcgtgc
     2461 tgggctgagg tgccgtgaaa cgtaaatcgt gaaacgtgaa tcgtgacgat cccaatctgg
     2521 gaatatcggt caatgattcc gggcgcggcg tggcccgcgg ttccggggca ggaaggcgca
     2581 aacctgctcg ccctgcaatt tcagctggag cagacccagt ggctatctcc cgaaaagctg
     2641 ggcgagctgc agttccgtca gcttgactcc ctggtgcgtc atgcgtacgg gactgtgccg
     2701 tattaccggg agcgctggcg cgggctctac gaccccggcg agagactcac ttccgagggc
     2761 tactcccggc tgccgctgct gacgcgcagg gagttgcaag ctaattttga tgcactgaaa
     2821 agcaccagca ttccggctgc tcatgggctg gtcacggaga gcaggacttc gggctccacc
     2881 ggcacgccgg tgcgggtgtt gaagacgggt tttgttgact tgctgtggca ggccatcttg
     2941 ctgagagagc atttgtggtt cgcgcgggac ctgaggggca agctggcttc gatccggcat
     3001 ggggcggcgg cgggcgaggc tgacggctgg ggacccgcga ccaccgcgat agtcggaacg
     3061 ggccgctcgg ccaccctgcc catcaaatcc gacgtggatg cccagttgca atggctcgaa
     3121 agccagcggc cggattacct gctgacttat ccttccaatc tcgccgagct ggccagacgc
     3181 tcgctggcgc gcggcatccg cctgccggcg ctgcgcgaag cgcgaacgtt cggtgagatg
     3241 ctgccaccgg agacgaggga attttgccgg agggcgtggg gcgtgccggt gaccgacacc
     3301 tattccacgc aagaagtcgg ctatatagcg ttgcaatgtc cgttgcacga gcactaccac
     3361 gtccagtccg agtgcgtcct gatggaagcg ctgaacgacg aggggctcgc gtgcgcgccg
     3421 ggtgcaacgg ggcgggtggt gattacctcg ctgcacaatt ttgcgatgcc gctggtgcgc
     3481 tacgatatcg gcgattatgc cgaagtcggc atgccgtgtg attgccgtcg tggcctgccg
     3541 gtgttgcgcc gtgtcgcggg tcgggtgcgg aatatgctgg ttctcgcaaa tggagcgcgc
     3601 tactggcccg gattgaatct gggaagcctc gcaggcggtg cgccagtact gcagcatcag
     3661 ctcgttcaaa aaagcttcga tacgattgag gttcgcctcg ttacagcacg tccgttgacc
     3721 ttggaagaag agcaacacat gcgtcagcat ttactggcca ggcttccggt tccgtttgaa
     3781 atcaatttca actattgcga cgcgatcagc cgcggtgccg gcggcaagtt cgaggagtgc
     3841 atatcagaaa tggctgcgac cgggtagctt ggaaatgatg cgcggcgcgg agaatggctt
     3901 tgcgcgcggc gttaccggct ctgcggccta cggggtaggc tatgaggtcc ggcggccccg
     3961 cgatgctaag atgagggtgt ccgggagttg cgccagtgat ggctgagtca cagctgattt
     4021 atgggttcca cgcggtgacg agccggttgc ggcagaacgc ggcgagcgtg aaggaaattt
     4081 acctcgacca gtcgcgcaac gatcggcggg cgaaggatct cgcgcagctg gcggcgcagc
     4141 gcggcgcgcg cgtgatgctg gtggacgggg ccaggctcga cggcatgaca cagcacgccc
     4201 gccaccaggg catcgtggcg cgggtggagc ccgccgccct ggcgagagac ctcgacgagg
     4261 tgctcgatgg gctcaaagag cccgctctgc tattgatcct cgacggcatc accgacccgc
     4321 ataatctcgg cgcctgcctg cgggtggcgg acgcgatggg cgtgcacgcc gtggttgcgc
     4381 ccaaggaccg cgcggccggc atcacgccca cggtcgagaa ggtggcgagc ggcgcagccg
     4441 agaccgtgcc cttcatcgcc gtcaccaatc tcgcgcgcac catgcgggag ctgaaggagc
     4501 gcgagatctg gctgatcggc gcggaccagg cggcggaacg cgacctctat tcgatcaaac
     4561 tcgatgtgcc gctcgcgtgg gtgctgggcg cggaaggcga aggcatgcgc aggctcacgc
     4621 gcgagaactg cgacgagctc gcgcgcatcc ccatgctcgg ggcggtggaa agcctcaacg
     4681 tgtcggtgtc ggccggcgtg tgcctggcgg aagcgcgccg tcagcgcgct ttccgtaaat
     4741 agacaggatc aatgattcga acacccgcca aacccgttaa ctgcatcaat ggacaaccgc
     4801 ccccatccta ccttcccccg caagcggggg aaggggataa ccaggattaa caaattcaaa
     4861 aaacaacaat cgaatcgccg aggcgcagag agaaccgggg ttagttaaag cttctgccct
     4921 tttgcgggtt ggcc
//
LOCUS       DBNGSG010000239         2275 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4320574, whole genome shotgun sequence.
ACCESSION   DBNGSG010000239 DBNGSG010000000
VERSION     DBNGSG010000239.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4320574"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="AC158Q_06305"
     CDS             complement(<1..336)
                     /locus_tag="AC158Q_06305"
                     /inference="COORDINATES: protein motif:HMM:NF019590.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0000423 - mitophagy [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HIR3339421.1"
                     /translation="MIYEFRTYDLKPHSLPEVEKRFAEGYEVRKKFSQLAAFWHTEIG
                     PLNQIIHVWPYKDLEERNRIRETAVKESGGKWPPKTAEFLVRQRSEIFIPFSFSPEMK
                     PGKQGPFFEM"
     gene            complement(575..1147)
                     /locus_tag="AC158Q_06310"
     CDS             complement(575..1147)
                     /locus_tag="AC158Q_06310"
                     /inference="COORDINATES: protein motif:HMM:NF039822.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HIR3339422.1"
                     /translation="MQKTLITFLSVLLTVGVAGAQAPERVRGTVTGVNGNTLSVKSSK
                     GNVDIVLAEKTSIVFTQPIAIEDINPGDFLGVTSQKAADGSLTAFEIRRFPKPLNPGH
                     RPFDGRDDQTMTNATVSTTVQATSGRELTLSYEGGSQKVVVSEKAAISTLVPGNRSQL
                     VPGAPVNLTARPGADGKLTALRIQVGPPRK"
     gene            complement(1266..1865)
                     /locus_tag="AC158Q_06315"
     CDS             complement(1266..1865)
                     /locus_tag="AC158Q_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339423.1"
                     /translation="MKSYCAVALAGMLALATAGADAQQKPQRIRGTITAFDGNVLSVK
                     SRDGKDLRIELTEKASVATARAITLADLKPGDYVGSTTLKRADGTLVAREVHMIPRSV
                     KEGHGPWDLEPGSMMTNANVASVVKAAEGQELTLEYSGGSQKILVPPGTPIVTTTPAD
                     RSFLKPGETVYFSAQVSADGKLTTSARIQVSKDGVRPPQ"
BASE COUNT          399 a          645 c          775 g          456 t
ORIGIN      
        1 catctcgaag aacggcccct gcttgcccgg cttcatctcg ggcgagaagg agaacggtat
       61 gaagatctcg gagcgctggc gcaccaggaa ttcagcggtc ttgggcggcc acttgccgcc
      121 gctttccttg accgcggtct cgcggatgcg gttgcgctcc tcgaggtcct tgtacggcca
      181 cacgtggatg atctggttga gcgggccgat ttcggtatgc cagaacgcgg cgagctggga
      241 gaacttcttg cggacttcgt agccttcggc gaagcgcttt tcgacttcgg gcaggctgtg
      301 cggcttcaga tcgtaggttc tgaattcgta gatcatggag gattcccttt ggacaggtct
      361 tggatggacg gcgcggcggc ggccgcgccc ggaaacaggc gggtaacttt actgcgaagc
      421 gcggcggttt gccagcgcta ccgtccacgg ggtgaacccc cgtggcaagg gaaaagggga
      481 aagggaaaag tgaaaagggc gacagttaca agagccaaac ccgcctgtcg tcctgcgtcc
      541 gccgcccccg gcgcatcaag gcggataggc cgggctactt gcgcggcggc ccgacttgta
      601 tgcgcagcgc ggtcagcttg ccgtcggcgc cagggcgggc ggtgaggttg acgggcgcgc
      661 cgggcacgag ttgcgagcga ttgcccggaa cgagcgtcga gatcgccgcc ttctcggaaa
      721 cgacaacctt ctgcgagccg ccctcgtagc tcaaagtgag ttcgcgcccg ctggtggcct
      781 gcaccgtcgt ggacaccgtc gcgttggtca tggtctggtc gtcgcggccg tcgaacgggc
      841 ggtgtccggg attcaggggc ttgggaaagc ggcgtatctc gaaggcggtc agactgccgt
      901 cggcggcctt ctgcgacgtg acaccgagga aatcgcccgg gttgatgtct tcgatcgcga
      961 tcggctgggt gaaaacgatc gaggtctttt cggcgagaac gatgtcgaca tttcctttgc
     1021 tcgacttcac ggagagcgtg ttgccgttga cgccggtgac tgtgccgcgc acccgctcgg
     1081 gcgcctgtgc gccggctacg cccacggtca gcaggacgga gaggaatgta atcagtgtct
     1141 tctgcatgcg acccccagtg gttgaaggga aaaggtaata gggaaaaggg aaacggggaa
     1201 agggtaggga aaggtcggaa aacggggtta ctttttcctt ttccttttcg acttttccct
     1261 tgacgtcatt gcggcgggcg gacgccgtcc ttgctcacct ggatgcgcgc cgaggtggtc
     1321 aacttgccgt cggcgctgac ctgggcggag aagtacacgg tctcgcccgg tttcaggaac
     1381 gagcggtccg ccggcgtggt cgtcacgatc ggcgtacccg gcggcaccag gattttctgc
     1441 gagccgcccg aatactccag ggtgagttcc tgtccttcgg cggccttgac gaccgatgcc
     1501 acattggcat tggtcatcat cgagccgggc tcgaggtccc acggtccgtg gccttccttg
     1561 acgctgcggg ggatcatgtg cacttcccgg gcaacgagcg ttccatcggc gcgctttaga
     1621 gtcgtggagc caacgtagtc gccgggcttg aggtcggcga gcgtgatcgc cctggcggtc
     1681 gcgacggaag ccttctcggt caattcgatc ctgaggtcct tgccgtcgcg cgacttcacc
     1741 gagagcacat tgccgtcgaa tgccgtaatg gtgccgcgga ttcgctgcgg cttttgctgc
     1801 gcgtcggctc cggcggtggc gagcgcgagc atgcccgcga gggctacggc gcaataagac
     1861 ttcatgggtg attcctccgg agtgaaggtg tgccggctgg ctgcctgggt aaacagggtt
     1921 gcgggccata atattccaga ttctggccga tcgaaggcaa atggcggaag cgacactgct
     1981 tgagaagaaa cctttgcgca ggtcgtcctc gcgaaggcgg ggacccagta atgttttgac
     2041 gatcctggat ttccgccttg gcgggaatga cgataccctg cctttcgact ttcgacttca
     2101 gcgaagcgtc aggtcatccc tcggaccacg gcctctgcta aagtgtcagc ttcatgtaag
     2161 gcaaagtaat ggctaagacc ctgaagatcg ggttgatggt tcccgccaac aacacgacga
     2221 tggaagggga actgctcgag tggctgccgc agggcgccac gtgccggacg ctgcg
//
LOCUS       DBNGSG010000240         5912 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3811465, whole genome shotgun sequence.
ACCESSION   DBNGSG010000240 DBNGSG010000000
VERSION     DBNGSG010000240.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5912)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5912)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5912
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3811465"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="AC158Q_06320"
     CDS             <1..850
                     /locus_tag="AC158Q_06320"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HIR3339424.1"
                     /translation="AGEALKLAGFDAVGNANNVNYGPEAIRASVARLKELGIPSTGAG
                     VNRRAAYAPVIIERNGARVGFLQRTSVYWPTNHEAGEHTPGVAVIRGNTAYQVPAHKT
                     RPEIPPMNRPGLPPIILTWAERDHLNRFQEEVAALRAQVDIVVCSNHWGLHQDVLDYM
                     TEIAHAAIDAGADIVVGHGPHYSLPVEIYRGKPIYYGLGSFSFHTGHGGRKHGDWVGM
                     LARVILDGRSVERASFRFARHSDANETVLCAPKDEPEALADIVKRSARFGTKIAMEGD
                     EAVFSA"
     gene            complement(858..2015)
                     /locus_tag="AC158Q_06325"
     CDS             complement(858..2015)
                     /locus_tag="AC158Q_06325"
                     /inference="COORDINATES: protein motif:HMM:NF024771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR3339425.1"
                     /translation="MKITAVRNYTVHPGWRKNLIFVKVETDAGIHGWGEAYSQYDRDT
                     AVTAQLDALGRYAVGRSPFDIKHFTQIAFDDYAQRRGSVELFCAISGIEQAMWDIVGK
                     ACKQPVYNLLGGRCRDKIRVYANGWGYQLKVPADYARAAEEVIKQGFTALKLDPLPAP
                     WRTWIPREEEERAVNVVKAVRAAVGPKVDILIDQHRRLAPMHAIRLDRRLAEFGLYWM
                     EESCQAEFPDELAQIRRETGLPMVIGEATYTKTGFRPLLEKRAADILNPDVACVGGIL
                     ELKEIAAMAEPFLVAMSPHNYNSTLVALASTVHASAVMPNFLITEYFVPFVDFCDRIS
                     PNQLKPKNGYIDLPTAPGLGVDVDEEALRKYPGKPYDTRKLRQPEDEPGGI"
     gene            2251..3120
                     /locus_tag="AC158Q_06330"
     CDS             2251..3120
                     /locus_tag="AC158Q_06330"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR3339426.1"
                     /translation="MTESVLRGVPPTETNLRDFVTALLPQGFAKIVQPIASTTISTDA
                     RGLTAGEVKIQTMTGPIPAYRAMPEKGSKFPLVLVVQEIFGVHEHNKDVCRRLAKLGY
                     FAIAVDLYHRQGDVTQFTDNQEIFAKVVNFVPDSQVMSDLDASVVYAESTGKADTSKL
                     GITGFCWGGRVTWTYCVHNPKVKAGVAWYGRLIAPAKAALQPAYPVELAQHLKVPVLG
                     LYGGADAGISIENVEQMRAALKAAGNTVSEIVVYDGAPHAFYADYRPSYRKEAADDGW
                     KRMQAWLKKYGVA"
     gene            complement(3121..4254)
                     /locus_tag="AC158Q_06335"
     CDS             complement(3121..4254)
                     /locus_tag="AC158Q_06335"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR3339427.1"
                     /translation="MHYVDSITGASYPLAVPRWRGDSGGHLNLADAPGLARNDIDTSL
                     NSLWRYRKALLINAADAVTLGEGWTPLVRGEWDGVPVEFKLEFMMPTGSFKDRGMTVL
                     VSYLRSRGVTSVLEDSSGNAGASLSAYCAAAGVRCRILVPATASYPKIAQIAACGADV
                     VTIKGSRQDVADAALAMSRDIFYASHNWQPFFAEGTKTLAYELWEQLGFRAPDNVVVP
                     VGYGANVLGCDRGCSELLRNREITRMPRLYGVQAANCAPYHAAYRAGVEHFVPVPVSP
                     TVAEGIASSKPTRMREVLAPVRASGGEIVAVTEDEIVRSLAALAKKGFYVEPTSAAAA
                     AGLSQLIARGVVRRGETTVLVLTGSGLKASETIGRLLGLKARD"
     gene            complement(4440..4733)
                     /locus_tag="AC158Q_06340"
     CDS             complement(4440..4733)
                     /locus_tag="AC158Q_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339428.1"
                     /translation="MSKLLTTLIAVAFAATTFGVAAQAPAPATPASPAAPAKGDATKA
                     TPATPATPSAKADKAKKTTKAKKTQGTTKKTEGKAKKSGGEGKKAPAKEGTTK"
     gene            5001..5780
                     /locus_tag="AC158Q_06345"
     CDS             5001..5780
                     /locus_tag="AC158Q_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017424363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase/reductase"
                     /protein_id="HIR3339429.1"
                     /translation="MDLKLTGRTALITGGSRGIGLGVAQALAAEGCHLHLSSRNAADL
                     EAARSKIVAAHKVNVTCHALDLGVSENARKLGQACRDVDILVNNAGAIPQGTITKLEE
                     KTWRDSWDLKVFGYINLTREIYAAMCERKRGVIVNIIGGAGERPSAGYIAGSMANAGL
                     MAFSRALGAEGPNFGVRVLGLNPSATATDRGVVRWRNQAQKDLGDPERWRELTKGFPF
                     GRPASVEEIADVVTFLCSDRASYVSGTVISVDGGAAARTNR"
BASE COUNT         1050 a         1944 c         1878 g         1040 t
ORIGIN      
        1 ggccggcgaa gcgctaaagc tcgccggttt tgacgccgtg ggcaatgcca acaacgtaaa
       61 ctacggcccc gaggcgatcc gtgcctcggt tgcgcgcctg aaggagctgg gcatcccgag
      121 caccggggcc ggcgtcaacc ggcgcgccgc gtatgcgccc gtgatcatcg agcgtaacgg
      181 cgcgcgcgtc ggatttctgc agcgcacgtc ggtctactgg ccgaccaatc atgaggcggg
      241 cgagcatacg ccgggcgtgg cggtaattcg cggcaacacc gcgtaccagg tgcctgcgca
      301 caagacgcga ccggaaatac ctcccatgaa ccgtcccgga ctgccgccca tcatccttac
      361 ctgggcggag cgtgatcatc tcaaccgctt ccaggaggaa gttgcggccc tgcgcgcgca
      421 ggtggatatc gtggtgtgct ccaatcactg gggactgcat caggatgtgc tcgattacat
      481 gaccgagatc gcgcatgctg cgatcgacgc cggtgccgac atcgtggtcg gccatgggcc
      541 gcactattcg ctgcccgttg agatatacag gggaaaaccg atctattacg gcctgggcag
      601 cttctctttc cacaccggac atggtggccg caagcatggt gactgggtag gtatgctcgc
      661 gcgcgtgata ctcgatggac gttcggtaga acgcgcttca ttccgttttg cgcggcacag
      721 cgacgccaat gagaccgtgc tgtgcgcgcc gaaggacgag ccggaggcgc tggcggatat
      781 cgtgaagcgc agcgcgcgct tcggcaccaa gatcgcgatg gagggcgacg aggcggtctt
      841 cagcgcctga ggacggctca gatgccgccg ggctcgtcct caggctgccg cagcttgcgc
      901 gtatcgtagg gtttgccggg atatttccgg agcgcttcct cgtcgacatc gacgcccaat
      961 cccggcgcgg tcggcagatc gatgtagccg ttcttcggct tcagctggtt gggcgagatc
     1021 ctgtcgcaga aatccacgaa cggcacaaaa tattcggtga tgaggaaatt tggcatcacc
     1081 gccgaggcgt gcacggtgga ggcgagagcc accagcgtcg agttgtaatt gtgcggcgac
     1141 atcgccacca ggaacggctc cgccatcgcg gcgatttcct tcagctccag gattccgccc
     1201 acgcaggcca catcgggatt caggatatcg gccgcgcgtt tctccagcag cggccggaac
     1261 ccggtcttgg tataggtcgc ttccccgatc accatcggca gtccggtttc gcgccggatc
     1321 tgcgccaact catcgggaaa ctcggcctgg cacgactctt ccatccagta gagcccgaat
     1381 tccgcgagcc gccggtcgag ccggatcgca tgcatcggcg cgagccgccg gtgctggtcg
     1441 atcaggatgt ccaccttggg ccccacggcg gcgcgcacgg ccttcacgac gtttaccgcg
     1501 cgctcttcct cttcccgtgg tatccaggtg cgccacggcg ccggaagagg atcgagcttc
     1561 aacgccgtga atccctgctt gataacctcc tctgccgcgc gcgcgtaatc cgccggcact
     1621 ttcaattgat agccccagcc gttggcatac acgcggatct tgtcgcggca gcgcccgccg
     1681 agcaggttgt agaccggctg cttgcacgcc ttgccaacga tgtcccacat ggcttgctcg
     1741 atgccggaga tcgcgcagaa tagctcgacc gagccgcgcc gttgcgcgta atcgtcgaaa
     1801 gcgatctggg tgaaatgctt gatgtcgaac gggctcctgc ccaccgcgta gcggccgagc
     1861 gcgtcgagtt gcgccgtgac cgcggtatcg cggtcgtact gggaataggc ctcgccccag
     1921 ccgtggatgc cggcgtcggt ctcgaccttg acgaaaatca gattcttgcg ccagccggga
     1981 tgtactgtgt agttcctgac cgcggtaatt ttcatattca atcccaagtt attcctgata
     2041 ttgcgcgccc attcctgcaa cagccggtcg gcgaattatg cactccgaca caaggccagg
     2101 ccatcgccgg gcggcgcagt tgccgcctgg gaatcgcata gtccacagta cgcactgcgc
     2161 gcccgccggc tggcgaggcg ggcgtaaata tatataattt gcgctccacg ccgccggtct
     2221 cgcggcgagc tttcaaccca ggaggtcctc atgactgaat ccgtgctgcg cggtgtgccg
     2281 ccaaccgaaa ccaacctgcg cgatttcgtg acagcattat tgccacaggg gttcgcgaag
     2341 atcgtgcagc cgatcgcttc caccacgatc tcgaccgacg cccggggcct caccgccggc
     2401 gaggtgaaga tccagaccat gaccggcccg atacccgctt accgggcaat gccggaaaag
     2461 gggagcaagt tcccgctggt gctggtggtt caggaaatct tcggtgtgca cgaacacaac
     2521 aaggacgtct gccgccgcct cgccaagctc ggttacttcg cgatcgccgt cgacctctac
     2581 caccgccagg gcgacgtcac ccagttcacc gataaccagg agatcttcgc caaggtcgtt
     2641 aacttcgtcc ccgacagcca ggtgatgtcg gacctggatg cctccgtcgt ctatgccgaa
     2701 tccaccggca aggccgacac ctccaaactg ggcatcaccg gtttctgctg gggcgggcgc
     2761 gtcacctgga cttattgcgt gcacaacccc aaagtaaagg cgggcgtcgc ctggtacggc
     2821 cgtctcatcg cacctgccaa ggccgcattg cagcctgcct acccggtgga actcgcgcag
     2881 catttgaagg tgccggtgct ggggctctac ggcggggccg acgccggcat ctccattgag
     2941 aacgtcgagc agatgcgcgc cgcgctcaaa gcggccggaa acacggtgag cgagatcgtc
     3001 gtgtacgatg gcgcgccgca cgctttctac gccgattacc gcccgagcta ccgcaaggaa
     3061 gcggcggacg acggctggaa gcgcatgcag gcgtggctca agaaatacgg ggtcgcctga
     3121 tcagtcgcgg gccttcaggc cgagtaatcg gccgatggtc tcggaggcct tcagccctga
     3181 gccggtgagc accagtaccg tcgtctcgcc gcgccggacg acgccgcggg cgatgagctg
     3241 cgacaggccg gcggcggcgg cggccgaagt cggttccacg taaaagccct tcttcgcaag
     3301 cgccgcgagc gagcgcacga tttcgtcctc ggtgactgcc acgatttcgc caccggaggc
     3361 acgaaccggc gcgaggacct ctcgcattcg tgtcggcttc gacgacgcga tcccttccgc
     3421 gacggtcggg ctgaccggga ccggaacgaa gtgctcaacg cccgcccggt atgcggcgtg
     3481 gtatggcgcg cagttcgccg cctgcacgcc gtagaggcgc ggcattcggg taatctcgcg
     3541 gttgcgcaac agctcggagc aaccgcggtc gcagcccagc acgttggcgc cgtagccgac
     3601 cgggactacc acgttatcgg gcgcgcgaaa gccgagttgc tcccacagct cgtaggcgag
     3661 cgtcttggtg ccttccgcga agaacggctg ccagttatgg ctggcgtaaa aaatgtcccg
     3721 gctcatggca agcgcggcgt cagctacgtc ctgtcgcgag cccttgatcg tgacgacgtc
     3781 ggcgccgcag gcggcaatct gcgcgatctt cggatacgac gccgtggccg gcacgaggat
     3841 gcggcagcgc acgcccgctg ctgcgcaata ggcggagagc gaggcgccgg cattgcccga
     3901 cgagtcctcc aggacagaag tgacgccgcg gctcctgaga tagctcacca gcacggtcat
     3961 gccgcggtcc ttgaatgagc ctgtcggcat catgaactcg agcttgaact cgaccggcac
     4021 gccatcccac tcgccgcgga ccagcggcgt ccagccttcg cccaaagtca ccgcgtccgc
     4081 ggcgttaatc agcaacgcct tgcggtagcg ccacagcgaa ttcagcgagg tatcgatgtc
     4141 gttacgggcg agccccggcg catcggcgag attgaggtgg ccgccggaat cgccacgcca
     4201 gcgcggcacg gcgaggggat agcttgcgcc ggttatcgag tcaacgtagt gcatgactgg
     4261 tgtctccgga accggtgcca tacccggtcc cgtgaaagcg agggtcagat tgtagcggtt
     4321 cgctgccccg ccggccggtg accccgccac ggcggacgcg cgcaaagaaa aaggcggctt
     4381 gcgccgcctt ttccgaaacg tctggcgaaa tttcgccgtc ggaacgaaag accagccggt
     4441 tacttcgtcg ttccttcctt ggccggggcc ttcttgcctt cacccccgga cttcttggcc
     4501 ttgccctcgg tcttcttggt tgtgccctgg gtcttcttgg ccttcgtcgt cttcttggcc
     4561 ttgtcggcct tcgcagaggg agtcgcgggg gtagccggcg tcgccttcgt ggcatcgccc
     4621 ttggccggtg ccgcggggga cgccggcgtt gcaggggccg gggcttgcgc cgcgacgccg
     4681 aaagtcgtcg ccgcaaaagc cacggcgatc agggtggtca gcagtttgct catgttttct
     4741 ccttgtcctc ggatgtgggt atgccggatt actctccggt cagagcgtat aacgcgcggc
     4801 cggcccgtcg gttgacttga agagtctgtc gtgcgagccc gacagattcc caggaaacag
     4861 gagaaaggcg cctttattcc gcacgaagtc ggcgaaacgg gagcaagtcc ggttcaatcc
     4921 cctggtacag tagaatagcg cgcccgcccg tccggcggcc ggacccgttc cccatccacc
     4981 tccacccata atcaatcgtc atggacctca aactgactgg ccgtactgca ttgatcaccg
     5041 gcggctcgcg cggcatcggc ctgggcgtcg cgcaagcgct cgccgccgag ggctgccatc
     5101 tgcacctttc ctcgcgaaac gccgccgacc tcgaagccgc acgcagcaag atcgtggcag
     5161 cgcacaaggt caacgtcacg tgccacgccc tcgacctcgg cgtgagcgag aacgcgcgga
     5221 aacttggcca ggcctgccgc gacgtcgaca tcctggtgaa caatgccggc gcgatcccgc
     5281 agggcacgat caccaagctc gaggaaaaga cctggcgcga ctcgtgggac ctgaaagtct
     5341 tcggctacat caacctgacg cgcgagatct acgcggcgat gtgcgagcgc aagcgcggag
     5401 tgatcgtcaa catcatcggc ggcgccggcg aacgcccgtc cgccggctat atcgccggca
     5461 gcatggcaaa cgccggactg atggcgttct cgcgcgcgct gggcgccgag ggcccgaact
     5521 tcggcgtgcg cgtactcgga ttgaacccca gcgcgaccgc gaccgatcgc ggggtcgtgc
     5581 gctggcgcaa ccaggcccag aaagacctgg gcgaccctga gcgctggcgc gagctgacca
     5641 agggctttcc gttcggccgg ccggccagcg tggaggaaat cgcggacgtc gtcacgttcc
     5701 tctgttccga tcgcgcttcc tacgtgtcgg gcaccgtcat ctcggtcgac ggcggcgccg
     5761 ccgcgcgaac caaccggtga cgaagcaacg cggtgacgga cacaggcgtc gcgcgtctcc
     5821 ccatcaattc atcaccccgt cactgtgatt tggcaggcgg cttctcgaaa gcccgatcca
     5881 cgcccttgag cgcctcgtcg tagcggccac gc
//
LOCUS       DBNGSG010000241         6904 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_926070, whole genome shotgun sequence.
ACCESSION   DBNGSG010000241 DBNGSG010000000
VERSION     DBNGSG010000241.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6904)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6904)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6904
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_926070"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2055
                     /locus_tag="AC158Q_06350"
     CDS             <1..2055
                     /locus_tag="AC158Q_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HIR3339430.1"
                     /translation="SCPKCGMALVSIAGTGEADDSELRDLTRRFWAGVALSIPLVVLA
                     MAPMVGIHELFGLAPRARGWVEFALGTPVVLWVGWPILHKFWLSLAHRSLNMYTLIGL
                     GVGLAYLFSLAAVLAPGLFPQEFREHDGAVGTYFEAAAVIVTLVMLGDVLQLRAMGQT
                     GQAIRQLLELAPSVAWRLRDDGTEEQVPLETVKAGDRLRVKPGEKVPVDGTVIEGASR
                     VDESMISGEPMPIAKEPGAKVTGATVNGNGSLVLRAERVGSETLLSQIVHMVAQAQRT
                     RAPIQKLADIIAAYFVQIVVAIAVVTAMAWWFFGPDPRLAYAFLNAVAVLIIACPCAV
                     GLATPISMTVAMGQGARAGILFRNAEAIERMRDIDTLVVDKTGTLTLGHPALTGFVVE
                     GIPENEALALVAGVEQLSEHPIGSAIVAGAKQRGLTVPAASAFTAVNGLGVRADVGGR
                     RVLVGSRGFLAQNGIDTKRWEERAESWRSEAKTVVFLAVDGSAAGIAAVADPVKENTP
                     EAIAALKKSGIRIVMLTGDSKRTAEAVARQLGIDETLAEVLPADKAGHVKRLQAEGRK
                     VAMAGDGINDAPALAQADVGIAMGTGTDVAMESAGVTLVKGDLRGIARAANLSRATMR
                     NIQQNLAFAFGYNALGIPIAAGVLYPFFGILLSPIFAGAAMAMSSVSVVTNALRLRKI
                     AL"
     gene            2230..2361
                     /locus_tag="AC158Q_06355"
     CDS             2230..2361
                     /locus_tag="AC158Q_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339431.1"
                     /translation="MNTLTSKLVFALALIAALSGCASTGPTESAVHWMDSQSVPVGD"
     gene            2465..3184
                     /locus_tag="AC158Q_06360"
     CDS             2465..3184
                     /locus_tag="AC158Q_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3339432.1"
                     /translation="MRNVLLIEDQQDIARLIQLHLRDLPCEVEVEYDGAAGLARAESR
                     NFDLVILDLMLPGIDGLEVCRRLRARPTYTPIMMVTAKAAEFDRVLGLEIGADDYLTK
                     PFSVRELAARVKALFRRIEAMADDRAPASNKAIRCGDLVIDPEKREVRLSDRPVNLTA
                     KEFDLLLHFARNRGRVYSRLQLLDTVWGYGHDGYEHNVNCHINRLRAKIEADHAQPKY
                     ILTVRGVGYKFAEAEAHSARA"
     gene            3187..4761
                     /locus_tag="AC158Q_06365"
     CDS             3187..4761
                     /locus_tag="AC158Q_06365"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3339433.1"
                     /translation="MTLYSKLVAVLLGFALIMAVMFLVAIRYSDLARNQEINQKFYGN
                     LASRMLGENVLDPDGTVSAATVQRVADRIQIINPRIDVYLLDAAGGIIAASGTDGVRR
                     HAVKLEPIRRFLADNPQLPIFGDDPSDGSRERVFSVAGVRLKENAQGYLYLVIRSRSG
                     DTLLQRVQNSYVLREMLLLIACGFLLTLLVSTVIIRFMTRRLQRLAQVMDRFRGSGYA
                     EQPARGALRSEAGDEIDQLAGTFNEMADRIHVQMEELRNTDSKRRELVANISHDLRTP
                     LASLQGHLETLQFKGDRLSPEEKRSYLETALRQSERLSHLVAKLFELAKLDSERAAVL
                     PEPFVLADLVQDVVQQFELAASGRSIRIAANLPLKMPLVLADIGLIERALRNLIENSL
                     RHTPPEGEIRVNVIPGAEHAIVQVSDTGCGIDPADLPRIFDRFYRGEKSRSDSSGNAG
                     LGLAIVKRILELHHSAVTVASEPGMTTIGFTLAYADSGHALPQAEAQSDPAAGDSKST
                     GPSVIPRPLKTRSAAW"
     gene            4965..5459
                     /locus_tag="AC158Q_06370"
     CDS             4965..5459
                     /locus_tag="AC158Q_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339434.1"
                     /translation="MDRISGNRNVLSIGLLAGLAGGAAEVAWVSLYSSATDTSGMTVA
                     QQVTASVAPVAAALPSAPLLGIAIHMLLSVALGLAFAATIWRLAAPRLGSAALLATAA
                     ASLALVWAFNFLVVLPALNPAFVMLMPLGATLASKLLFGIAMGSVLLMSSPRLPAGNP
                     RAFQ"
     gene            5550..5843
                     /locus_tag="AC158Q_06375"
     CDS             5550..5843
                     /locus_tag="AC158Q_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3339435.1"
                     /translation="MNKMTLMVLAAAGTLTAGAGLAQSGADVVKAKGCLNCLEVDKKK
                     VGPAFKDTAAKNKGDKGAEAKLVAMLKAGTAHPKVAASDAELKAAVQYVLSTK"
     gene            6122..>6904
                     /locus_tag="AC158Q_06380"
     CDS             6122..>6904
                     /locus_tag="AC158Q_06380"
                     /inference="COORDINATES: protein motif:HMM:NF021010.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HIR3339436.1"
                     /translation="MSVASIFAGSGAFAADPASINWKNVPTKSVTLFYPGQSTFQWLR
                     SPEHPGAQMVNTGGACLTCHKGTEQKLGDKLVKANKLEPTPIDGKNGTVQLSFQVAYD
                     KENAYFRFQWKTNSPHPGEAYPFLRFDGKEWKNFGNQRLAGPVRKGEQPAVYEDRLSI
                     MLDDGGVPNFATQGCWQTCHNGLRDAPNQPKTDEVQANPLLKALKKNDVRKYLPSTRG
                     DALASWDQGKSLEEIAKVKAAGGFLELMQWRAHRSNPVGMADD"
BASE COUNT         1259 a         2195 c         2247 g         1203 t
ORIGIN      
        1 agctgcccca agtgcggcat ggcgctggtg tcgatcgccg gcaccggtga agcggatgat
       61 tcagagctgc gcgacctcac acggcggttc tgggcgggcg tcgcgctatc gattccgctc
      121 gtggtgcttg cgatggcgcc gatggtcggc atacacgagc tcttcggcct cgcgccgcgg
      181 gcgcgcggct gggtcgagtt cgcgctcggc acgccggtgg tgctgtgggt cggctggccg
      241 atcctgcaca agttctggct gtcgctcgcg caccgctcgc tcaacatgta cacgctgatc
      301 ggcctcgggg tcgggcttgc ctacctgttc agtctcgccg cggtgctcgc gccgggcctg
      361 ttcccgcagg agtttcgcga gcacgatggc gcggtcggca cctacttcga ggcggcggcc
      421 gtgatcgtga ccctggtgat gctcggcgac gtcctgcagt tgcgcgcgat gggccagacc
      481 ggccaggcga tccggcagtt gctggagctc gcccccagcg tggcatggcg gttgcgtgac
      541 gatgggaccg aggaacaggt gccgctcgaa acggtgaagg ccggcgaccg gttgcgcgtg
      601 aaaccgggtg agaaggtgcc ggtggacggc acggtgatcg agggcgcgag ccgggtggat
      661 gagtccatga tctccggtga accgatgccg atcgccaagg aaccgggcgc gaaggtcacc
      721 ggcgctactg tgaatggcaa cggctcgctc gtcctccgcg ccgagcgggt tggctcggaa
      781 accctgctct cgcagatcgt gcacatggtc gcccaggccc agcgcacgcg cgcgccgatc
      841 cagaaactcg ccgacatcat cgcggcgtat ttcgtccaga tcgtggtggc catcgccgtc
      901 gtgacggcga tggcatggtg gttcttcggt ccggatccca ggctggctta cgcatttctg
      961 aacgcggttg ccgtattgat catcgcctgc ccctgtgccg ttggcctcgc tacgcccatc
     1021 tccatgaccg ttgccatggg gcaaggcgcg cgggcgggaa ttctgtttcg caacgccgag
     1081 gccatcgagc gcatgcgcga catcgacacg ctggtggtgg acaagaccgg cacgttgacg
     1141 ctcgggcatc ccgcactgac cggcttcgtg gtcgagggca ttccggaaaa tgaagcgctt
     1201 gcgctcgtcg ccggcgtgga acagctttcc gagcatccga tcgggtcagc catcgtcgcg
     1261 ggcgcgaagc agcgcggtct gaccgtgcct gcggccagcg cgttcacagc agtgaacggg
     1321 ctcggcgtgc gggccgatgt cggcggcagg cgcgtcctgg tgggaagccg cgggtttctt
     1381 gcgcaaaacg gcatcgacac gaaacgctgg gaggagcgcg cggaatcctg gcgcagcgaa
     1441 gcgaaaaccg tcgtgttcct cgcggtggac ggaagcgcgg ccggcatcgc cgcggtcgcc
     1501 gatccggtca aggagaacac gcccgaggcc attgcggcgc tcaagaaatc cgggatacgg
     1561 atcgtcatgc tgaccggcga ttcaaagcgc acggccgaag ccgtcgcccg gcagctcggc
     1621 atcgacgaga cgctcgcgga agtcctgccc gcggacaagg ccgggcacgt gaaacgcctg
     1681 caggccgaag ggcgcaaggt cgcgatggcg ggcgacggca tcaacgacgc gccggcgctc
     1741 gcgcaagccg acgttggcat cgccatggga accggcaccg atgtcgccat ggaaagcgcc
     1801 ggcgtgacgc tggtcaaggg cgatctccgc ggcatcgcgc gcgccgcgaa tctctcgcgc
     1861 gccaccatgc gcaatatcca gcagaacctg gcgtttgcat tcggctacaa cgcgctcggc
     1921 atcccgatcg ccgccggggt cctctatccc ttcttcggca tcctgctctc ccccatcttc
     1981 gccggcgcgg cgatggcgat gagttcggtc tccgtcgtca ccaacgcgct gcggctgcgt
     2041 aaaattgccc tttgaagcgc cgcagcgctt ctgccagccg ttttcgagac gaaaaaacga
     2101 tatagatgcg atgaagtagc gattccgggc ttccatactg taatgagaat acgatgaatt
     2161 tcatcgaaga ttcaacgata tgtcgtattc cccatgatcc cttgttccac tacggtaaag
     2221 gaaacccata tgaatacgct gacaagcaag ctggtttttg ccctggcgct gatcgccgcg
     2281 ttgtccgggt gcgcgagcac cgggcccacc gaatcggcgg tacactggat ggattcgcag
     2341 tccgtaccgg tcggggattg aacttgccgc atcgtggcct ccgcggagga cacgagtttt
     2401 tgcatctcct gatatccttc ccgccggtcg acgggcgcgc agcgggaatt gaccggcttg
     2461 aataatgcgc aacgtcctcc tcatcgaaga ccagcaggat atcgccaggc tgatccagct
     2521 ccacttgcgc gacctgccgt gcgaggttga ggtcgaatac gacggcgccg ccggtcttgc
     2581 ccgggccgag tcccggaact tcgatctcgt catcctcgac ctgatgctgc ccggcatcga
     2641 tgggctggag gtgtgccggc ggctgcgcgc ccgcccgacc tataccccga tcatgatggt
     2701 cacggcgaag gcggccgaat tcgaccgggt gctgggactc gaaatcggag ccgacgacta
     2761 tctcaccaag ccgttcagcg tgcgcgagct ggcggcgagg gtcaaagcgc tgttcaggcg
     2821 catcgaggcg atggccgacg atcgtgctcc cgcctccaac aaggcgatcc gttgcggcga
     2881 tctcgtcatt gaccccgaaa agcgcgaagt ccggctgagc gaccggcccg tcaacctgac
     2941 cgccaaggaa ttcgatctgt tgctgcactt cgcgcgcaac cgcgggcgcg tctacagccg
     3001 gctgcaactg ctcgacacgg tatggggcta cggtcacgac gggtatgagc acaacgtcaa
     3061 ctgccacatc aaccggctgc gcgccaagat cgaggccgac cacgcgcagc cgaagtacat
     3121 attgacggtg cgcggcgtgg gctacaagtt cgccgaggcc gaagcccact ccgcccgggc
     3181 ctgaccgtga cgctctacag caagctcgta gcggtccttc tcgggttcgc actgatcatg
     3241 gcggtgatgt tcctggtcgc aatccgctat tcggacctcg cgcgcaacca ggaaatcaac
     3301 cagaagttct acggcaatct cgcgagccgc atgctcgggg agaacgtgct ggacccggac
     3361 ggcacggtca gcgctgccac ggtgcaacgg gtggcggacc gcatacagat catcaatccg
     3421 agaatcgacg tctatctgct ggatgcggcc ggcgggatca tcgccgcatc cggcacggac
     3481 ggggtcaggc gtcacgcagt gaagctggaa cctatcaggc gcttcctggc ggataacccc
     3541 caattgccca ttttcggcga cgatccctcc gatgggagcc gcgaacgcgt cttctccgtg
     3601 gctggcgtgc gcctcaagga aaacgcccag ggctatctct atctcgtcat tcgcagccgg
     3661 agcggggata cgcttttgca gcgcgtgcag aacagctacg tgctgcgcga aatgctgctg
     3721 ctcatcgcct gcggcttcct gctcacgctg ctggtgagca ccgtcatcat ccggttcatg
     3781 acgcggcgcc tgcagcggct tgcgcaggtg atggaccggt tccgcggcag cggctatgcc
     3841 gagcagccgg cgcgcggcgc gctgcgctcg gaagccgggg acgaaataga ccagctcgcg
     3901 ggcaccttca acgagatggc cgatcgcatc cacgtccaga tggaggagct caggaatacc
     3961 gacagcaagc gccgcgagct ggtcgccaac atctcgcacg acctgagaac gccgctggcg
     4021 tccctgcagg ggcatctcga aacgctgcaa ttcaagggtg accgcctctc gcccgaggaa
     4081 aagcgcagct atctcgaaac cgcgctgcgg cagagcgagc ggttgagcca cctcgtcgcc
     4141 aagctgttcg agctcgcaaa gctcgattcc gagcgggcgg cggtcctgcc cgaaccgttc
     4201 gtgctcgccg acctggtgca ggacgtggtg cagcaattcg agctggcggc atcgggcagg
     4261 agcatccgca tcgccgcaaa cctgccgctg aagatgccgc tggtgctcgc cgatatcggg
     4321 ctcatcgagc gcgcgctccg gaacctgatc gagaattcgt tgcgccacac cccgccggag
     4381 ggcgaaatca gggtgaacgt gatccccggc gcggagcatg caatcgtgca ggtgtccgat
     4441 accggctgcg gcatcgaccc cgcggacctc ccgcgcatct tcgatcgctt ctaccgcggc
     4501 gagaagagcc gcagcgattc ttccggcaac gccggtctcg gcctcgcgat cgtcaagcgg
     4561 atcctggaac tgcaccacag cgccgtcact gtcgcgagcg agccgggcat gacgacgatc
     4621 ggttttacgc tcgcctacgc cgatagcggt cacgccttgc cgcaagccga agcccaatcc
     4681 gatcccgcag ccggtgacag caaatcaacc gggccttccg tcattccgcg gcccctgaaa
     4741 acaaggtcgg cggcctggta gagccatgcc gcgcggcttt attattttga agctggagtt
     4801 cggggccgcc ccctgcccct tccatgcggg ggctctcgcc gtcggaaatg cgtcccttgc
     4861 ggcgcatctt gatccagatc aaaatcgtcg gcagcgcgca tggcatgctg gttaggaacc
     4921 gggacaaaca tcgcggacgg ctattttctt gataaggggg gctcatggat cggatttccg
     4981 gcaacaggaa cgtattgtcg atcggattgc tcgccggctt ggccgggggc gccgctgaag
     5041 tggcgtgggt gagcctgtac agcagcgcca cggacaccag cgggatgacc gtcgcgcagc
     5101 aggtcacggc gagcgtggcg ccggttgctg cggcgctgcc ttccgcgcct cttttgggta
     5161 ttgcaattca catgctgctc tccgtcgcgc tgggcctcgc ctttgcagcc acgatctggc
     5221 gcctggccgc gccgcggctc ggtagcgccg ccctcctggc aaccgccgcc gcctcgctgg
     5281 cgctggtctg ggcgttcaac tttttggtcg tgctgcccgc gctcaatccg gcgttcgtca
     5341 tgctcatgcc gctcggcgcc accctggcgt ccaagctcct gttcgggatc gccatgggct
     5401 cggtgctgct gatgtcctcg ccaaggcttc cagcaggaaa tccgcgcgcg ttccagtagc
     5461 gggagtttga tgcggacggg cggtgcatct aatactgccc gcagtcatgt aatgccgggc
     5521 gtttcgcaac tctcaaatcg gagatgacaa tgaacaagat gacgctgatg gtgctggccg
     5581 ccgcgggaac gctgacggcg ggcgctggcc tcgcgcaatc gggcgccgat gtcgtgaaag
     5641 ccaagggctg cctcaactgt ctcgaggtgg acaagaaaaa ggtcggcccg gcattcaagg
     5701 acaccgccgc caagaacaag ggcgacaagg gcgccgaagc caagctggtc gcaatgctca
     5761 aggccggcac ggcgcacccg aaagtcgcgg cttcggacgc cgaactcaag gcagcggtgc
     5821 aatacgtgct gtcgacgaag taatagcagt cggtcaggca atacgcgggt ttacagcctg
     5881 atcgttattt caggtccccc gcaatcgaca cgccgaaatg tcagcccctt gttcggaaaa
     5941 gggaattcga acggctaatt gattcagatc aaatgacccg ggcagtctgg ctgctaatgt
     6001 cggccctgaa ggtgccgggt tgcgtttccg aggtgagcca aatcggctcg ctccagatgc
     6061 cggattgtga aattcaactg ttggagggat tatgaaagcg ttcggattgg caactgcagc
     6121 gatgagcgtt gcgtcgattt tcgcgggttc cggggcgttt gctgccgatc cggcgagcat
     6181 caactggaaa aacgtcccga cgaaatcggt gacgctattc tatccaggtc aatcgacctt
     6241 ccagtggctg cgcagtcccg aacacccggg cgcgcagatg gtcaacaccg gcggagcctg
     6301 cctgacgtgc cacaagggca cggagcagaa gctgggcgac aagctcgtca aggcgaacaa
     6361 gctggaaccg acgccgatcg acggcaagaa cggcaccgtc cagttgagct tccaggttgc
     6421 ctacgacaag gagaacgcct atttccggtt ccagtggaag acgaacagtc cgcatcccgg
     6481 cgaggcctat ccgttcctgc gcttcgacgg caaggagtgg aagaatttcg gcaaccagcg
     6541 gctggccggc ccggtccgca agggcgagca gcccgcggtc tatgaagacc gcctgtcgat
     6601 catgctcgac gacggcggcg tgcccaactt cgcgacacag ggctgctggc agacctgcca
     6661 taacggcctg cgcgacgcgc cgaaccagcc gaagaccgac gaggtgcagg ccaacccgct
     6721 gttgaaggca ctgaagaaga acgacgtccg caagtacctg ccctcgacgc gcggcgacgc
     6781 gctggcttcc tgggaccagg gcaagagcct ggaggaaatc gccaaggtca aggcggccgg
     6841 gggcttcctc gagctgatgc agtggcgcgc ccaccgctcc aacccggtcg gcatggcgga
     6901 tgac
//
LOCUS       DBNGSG010000242         5540 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1775318, whole genome shotgun sequence.
ACCESSION   DBNGSG010000242 DBNGSG010000000
VERSION     DBNGSG010000242.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5540)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5540)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5540
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1775318"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="AC158Q_06385"
     CDS             complement(<1..290)
                     /locus_tag="AC158Q_06385"
                     /inference="COORDINATES: protein motif:HMM:NF046799.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR3339437.1"
                     /translation="MLSKLQQFGLLVIGVLIGVSISLNYAAEAQRATTAAPLPIEELR
                     AFTEVFGRVKSDYVEPVDDKKLITEAINGMLTGLDPHSAYLDQEAFRELQVGT"
     gene            complement(392..1570)
                     /locus_tag="AC158Q_06390"
     CDS             complement(392..1570)
                     /locus_tag="AC158Q_06390"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC family protein"
                     /protein_id="HIR3339438.1"
                     /translation="MSARAFGLLILLLLASPAAHPAGAGTKEKELRELRGRIEALQKR
                     LAESEESKSEAADALRESERAISETNRELRELGAQSREINQRLAELGAESRRQEEALK
                     AQQERLAQLLYQHYLGGESEPLKLLLNREDPNDIARRLHYFGYISRARARLIVETRES
                     LARLQELARETGQKASELAVVTAESKALRARLEREKRARGQVLTRISRNIQRQRREIG
                     TLKRDETRLTRLIESLAKLMARSKPTPRLRNERVPQASSGDSPFLELKGKLNLPVRGE
                     LANRFGSPRADGGVVWKGLFIAARAGEEVRAVADGRVVFADWLRGFGNLLIVDHGDAY
                     MSLYGNNEALYKQVGDPIRGGEPVAAVGASGGNTDSGLYFELRHQGRPLDPLDWVGTR
                     "
     gene            complement(1567..3096)
                     /gene="gpmI"
                     /locus_tag="AC158Q_06395"
     CDS             complement(1567..3096)
                     /gene="gpmI"
                     /locus_tag="AC158Q_06395"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HIR3339439.1"
                     /translation="MKVTPVLLIILDGFGHREECEHNAICQARKPHWNVLWQTCPHTF
                     IDASEKWVGLPRNQMGNSEVGHMNIGAGRVVYQDYTRIENAIETGEFFGNPALLKAME
                     SARGAGRALHVLGLLSPGGVHSHESQIHSLLEMAARAGVKDVRLHAFLDGRDTPPKSA
                     EASLAALQKKCGELKIGRIASLCGRYYAMDRDNRWERVQTAYDLITQGKAEFRAGTAA
                     EGLEAAYARGETDEFVSATVIGSPERMNDGDAVVFMNFRADRARQLTRALTDAGFKDF
                     KRGHLPRLGAFCTLTSYGEDFAHIPAAFPPQSVAGGFGEIISGQGLKQLRIAETEKYA
                     HVTYFFNGGVEQPYPGEDRTLVPSPRVATYDLKPEMSAYEVTDRLVEAIRSRKYQAII
                     CNFANSDMVGHTGNLAAATRAIEVLDECIGRAVTAMRDIGGEVLITADHGNAETMLDP
                     ETKQAHTAHTLNLVPLLYVGRRATAADRGALQDVAPTLLAMMGLPQPAQMTGKSLLTF
                     T"
     gene            3165..4901
                     /locus_tag="AC158Q_06400"
     CDS             3165..4901
                     /locus_tag="AC158Q_06400"
                     /inference="COORDINATES: protein motif:HMM:NF023274.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PglL family O-oligosaccharyltransferase"
                     /protein_id="HIR3339440.1"
                     /translation="MLAGLAWTVPFLQPHHRYPLTGFYSEWLAFALGLAAALLLLGRE
                     AWRESKLPAVAIAPVGLAALLGLQVALGRVPYPEQALTAVLYLLWASLLILLGHVLRR
                     TLTLTAVATVLAWFLLAGGILSALVGLLQHLQVSTPFDFLVARNLARQAYGNVGQPNH
                     HAAYLSFALVSVIYLYSRRNLHGMLAAGCAALFLLTLTLTASRSPWIYLGAFTLMAWL
                     LHRLRPGADSRRLAVVALWLLPGFMVAQVVAALPFMASPGGVAASSLERVFQLASGVD
                     SRLQLWGEAWWMFLGAPLLGAGWGQFSWQHFLSQAATDAAVAPGVFNHAHNIVLQLMA
                     ETGLVGALIIVGSIGLWASDLRRVRFELEWWWLLALAAVIGIHSLLEYPLWYSYFLGV
                     AALLLGLGAERTFALRFAAGARAVAGLVMVAGWLNLVAMPPTYREFERLVFTPASRAA
                     QLPDEEAFAKAVVGLYREPLLVPYVELAIAYGVTVSVEKLPEKLALTARAVHFAPVPV
                     VAYRYALLLALAGEREAALVQLERSLRVYPEGAGDVAAHLKTLARDRPGEFLPLLKRV
                     ADWRDGRRERTQ"
     gene            5008..5442
                     /locus_tag="AC158Q_06405"
     CDS             5008..5442
                     /locus_tag="AC158Q_06405"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR3339441.1"
                     /translation="MEQFLLFLQKSPFNMLLLGLAVSSGGMLLWPLVSRPFRQTHEVG
                     AFEAVQLINRRDAVVLDVRDTGDHAAGHITNARHVPEAQLAGRMKELDKYKSRPIVVS
                     CRTGSRAPGVAGMLRKQGFAEAFALRGGIAAWQQANLPLEKG"
     gene            5453..>5540
                     /locus_tag="AC158Q_06410"
     CDS             5453..>5540
                     /locus_tag="AC158Q_06410"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HIR3339442.1"
                     /translation="MARITMYSTGVCPFCQMAERLLRSKGVSV"
BASE COUNT          791 a         1835 c         1860 g         1054 t
ORIGIN      
        1 gtgccgacct gcaactccct gaacgcttcc tggtcgagat aagcggagtg agggtcgagc
       61 ccggtcagca tgccgttgat cgcctcggtg atgagcttct tgtcgtccac cggctcgacg
      121 tagtcgctct tgacgcgccc gaacacctcg gtgaacgcgc gcagttcctc gatcggcagc
      181 ggcgcggccg tcgtcgcccg ttgcgcctcg gccgcatagt tgaggctgat cgaaacgccg
      241 atcagcacgc cgataaccag cagcccgaat tgctgaagct tgctaagcat tgctactcct
      301 tgaaacccgc gaatcgtgaa ccgtgagccg ggaaaaccgc acccaacaag ccctaagcgg
      361 ctgttgccgc tcgccaacag ctgccgaccg cttaccgggt gccgacccag tccaaggggt
      421 ccagcggcct gccttgatgc ctgagctcaa agtataaacc cgaatcggtg ttgccgccgc
      481 tcgcgcccac cgcggcgacc ggctcgccgc cgcgaatcgg gtcgccgacc tgcttgtaga
      541 gggcctcgtt gttgccgtag aggctcatgt aggcgtcgcc atggtcgacg atgagcaggt
      601 tgccgaaacc gcgcagccag tcggcgaaca ccacgcgccc atcggcaacc gcccgcactt
      661 cctcgcccgc ccgggcggcg atgaacaatc ccttccagac tactccgcca tcagcccgtg
      721 gactgccgaa acggttcgca agttccccgc ggaccggaag attgagcttt cccttcagct
      781 ccaggaaggg gctgtccccg ctggaggcct gcggcactcg ctcgtttcgc agccgggggg
      841 tgggcttgga gcgcgccatc agcttggcca ggctctcgat gagccgcgtg agccgggtct
      901 cgtcgcgctt caacgtgcca atctcgcgcc gctggcgctg gatgttgcgg gagatccggg
      961 tcagcacctg gccgcgggct cgcttctcgc gctccaggcg cgcgcgcagc gccttgcttt
     1021 cggcggtgac gacggcaagt tccgatgcct tctgcccggt ttccctggcg agctcctgca
     1081 gccgcgcgag gctctcgcgc gtctccacga tgagccgcgc gcgcgcgcgg gagatgtagc
     1141 cgaagtagtg caggcggcgc gcgatatcgt tggggtcctc gcgattcaac aggagcttca
     1201 gcggctcgga ttccccgccc aggtaatgct ggtagagcag ttgcgccagc cgttcctgct
     1261 gcgctttcag cgcctcctcc tggcggcgcg actcggcacc gagctccgcg agccgctggt
     1321 tgatttcccg ggactgggcg ccgagctcgc gcagctcgcg gttcgtctcc gagatcgcgc
     1381 gctccgattc cctcagggcg tcggccgcct cgcttttcga ctcctccgac tcggcgaggc
     1441 gtttctgcag cgcctcgatg cgtccgcgca gctcgcgcag ctccttttcc ttcgtgccgg
     1501 ctcccgccgg atgcgccgcg gggctcgcga gcagcagcag tatcagcagg ccgaaggcgc
     1561 gggcgctcac gtgaaggtga ggagggactt tccggtcatc tgcgccggtt gcggcaggcc
     1621 catcatggcg agcagcgtgg gcgcgacgtc ctgcagcgca ccgcggtccg ccgccgtcgc
     1681 gcggcgtccg acgtagagca gcggaacgag gttcagcgtg tgcgcggtgt gggcctgctt
     1741 cgtctcgggg tcgagcatgg tctcggcgtt gccgtggtcg gccgtgatca gcacctcgcc
     1801 gccgatgtcg cgcatggcgg tgaccgcgcg cccgatgcac tcgtcgagca cctcgatcgc
     1861 gcgggtggcg gccgcgaggt tgccggtatg gccgaccatg tcgctgttgg cgaaattgca
     1921 gatgatggcc tggtatttcc ggctgcggat cgcctccacc agcctgtccg ttacctcgta
     1981 ggcgctcatc tcgggcttga ggtcgtaggt ggcgaccctg ggcgagggca cgagcgtgcg
     2041 gtcttcaccc gggtacggct gctccacgcc gccgttgaag aagtaggtca cgtgcgcgta
     2101 cttctcggtc tccgcgatgc gcagctgttt caacccctgc cccgaaatga tttcgccgaa
     2161 gccgcccgcg acgctctgcg gcggaaacgc ggccgggatg tgggcaaaat cctcgccgta
     2221 gctcgtcagc gtgcagaaag cgccaagcct gggaagatgc ccgcgtttga aatccttgaa
     2281 accggcgtcg gtgagggcgc gcgtcaactg ccgggcgcgg tcggcgcgga aattcatgaa
     2341 cacgaccgcg tcgccgtcgt tcatgcgctc cggggacccg atcaccgtcg cgcttacgaa
     2401 ctcgtcggtc tcgccgcgcg cgtaggcggc ctccaacccc tccgcggcgg tcccggcgcg
     2461 gaattccgct ttcccctgcg tgatgaggtc gtaggcggtc tgcacgcgct cccaccggtt
     2521 gtcgcggtcc attgcgtagt agcggccaca gagcgaggcg atgcggccga ttttcagttc
     2581 gccgcatttc ttctggagcg cggcgagcga ggcttcggcg ctcttcggcg gggtgtcgcg
     2641 gccgtcgagg aaagcgtgca gccgcacgtc cttcacgccg gcgcgcgccg ccatctcgag
     2701 cagagaatgg atttgtgact cgtggctgtg cacgccgccg ggggagagca ggcccaggac
     2761 gtgcagcgcg cgcccggcgc cgcgcgcgga ctccatcgcc ttcaggagcg ccggattgcc
     2821 gaaaaattca ccggtctcga tcgcgttctc gatccgggtg taatcctggt aaacaacgcg
     2881 gccggcgccg atgttcatgt gcccgacctc cgaattgccc atctggttcc tgggcagtcc
     2941 cacccatttt tccgacgcgt cgatgaaggt gtggggacag gtctgccaca ggacgttcca
     3001 gtgcggcttg cgcgcctggc agatggcgtt gtgctcgcat tcctcgcggt gcccgaaacc
     3061 gtcgagtatg atcagcagca ccggggttac tttcatgatt tcacggactt cctggcgtgg
     3121 catcgtctcc agccgatttt aaactatttg cccgcgccag cctgatgctg gccggactcg
     3181 catggacggt tccgttcctg cagccccacc accgataccc gctcaccggc ttctacagcg
     3241 agtggcttgc gttcgcgctg ggactggccg cggcgctgtt gctgctcggc cgggaagcgt
     3301 ggcgggagtc gaagctgccg gcggtggcga ttgccccggt cgggttggcc gcgttgctgg
     3361 ggctgcaggt ggcgctcgga cgcgtgccgt acccggagca ggcgctgacc gccgtactgt
     3421 acctgctgtg ggcgtcattg ctgatcctgc ttggccacgt attgaggcgc acattgacgc
     3481 tgacggccgt cgcgacggtc ctcgcctggt tcctgctggc gggaggtatc ctcagcgcgc
     3541 tggtcggcct gctgcagcac ttgcaggttt ccacgccatt cgatttcctg gtcgcgcgca
     3601 acctggcgcg ccaggcgtat gggaatgtcg gccagcccaa tcaccacgcc gcctacttgt
     3661 ctttcgccct ggtctcggtg atctatctgt acagccgccg taacctgcac ggcatgctgg
     3721 cggcgggttg cgccgcgctg tttctgctga cgctgacgct gaccgcgtca cgcagcccct
     3781 ggatttatct cggcgcgttc accctgatgg cctggctgct gcatcgcctc cgtcccgggg
     3841 ccgatagccg caggctggcc gtcgtggcgc tgtggctgct gcccgggttc atggttgcgc
     3901 aggtcgtcgc ggcactccca ttcatggcgt cacctggagg cgtggcggct tcctccctgg
     3961 aacgcgtgtt tcagcttgcc agcggggtgg attcgaggtt gcagctctgg ggcgaggcct
     4021 ggtggatgtt tctcggcgct ccgttgcttg gtgccggctg gggacagttt tcctggcaac
     4081 acttcctgag ccaggccgcg accgatgcag cggtggcgcc cggcgtattc aaccatgccc
     4141 acaatatcgt gctgcagctc atggccgaaa ccgggctggt tggcgcgctg atcatcgttg
     4201 gttcgatcgg gctttgggcg tccgatttga ggcgcgtcag gttcgaactc gagtggtggt
     4261 ggttgctcgc gctcgccgcg gtcatcggta tccacagcct gctcgagtac ccgttatggt
     4321 actcgtactt cctcggggtc gcggcgttgc tgctgggcct cggcgcggaa cgcacgtttg
     4381 ccctgcggtt cgccgccggc gcgcgcgccg tggcgggact ggtcatggtc gcggggtggt
     4441 tgaatctggt cgccatgccg ccgacgtacc gcgagttcga gcgcctggtg ttcaccccgg
     4501 caagccgcgc ggcgcagctg ccggacgagg aggcattcgc caaagccgtc gtcggcctgt
     4561 accgtgagcc gctgctcgtc ccgtacgtgg aactggccat tgcgtacggc gttacggtca
     4621 gcgtggaaaa gctcccggag aaactcgcgc ttactgcccg cgccgtgcat ttcgcgccgg
     4681 taccggtcgt ggcctatcgc tatgcgctgc tgcttgcgct ggccggcgag cgcgaagcgg
     4741 cgctcgtcca gctcgagcgg tcgttgcgcg tgtatcccga aggggcgggc gatgtcgccg
     4801 ctcacctcaa aacgctagcg cgcgaccggc ccggcgaatt ccttcccctg ctgaaacggg
     4861 tggcggactg gagggacggc cgccgcgagc gaacacaatg aatgtcatga atcccgccgg
     4921 acgggcgcct ctgataaaat cagcctccgt ccagcccgcg caacggcacg cgcagcggga
     4981 ccggcggcaa cgtaaaggat gaatggagtg gagcagttcc tgctttttct gcagaagagc
     5041 ccgtttaaca tgctgctgct cgggttggcg gtttcatcgg gcggcatgct cctgtggccg
     5101 ctcgtctcgc ggccgttccg gcagacccac gaagtgggcg ctttcgaggc ggttcagctc
     5161 atcaaccggc gcgatgcggt agtgctcgac gtgcgcgata ccggggacca cgcggccggg
     5221 cacatcacca acgccaggca cgttcccgaa gcgcagctgg ccggacgcat gaaagaactc
     5281 gacaagtaca agtcgcgccc catcgtcgtc agttgcagga ccggctcccg ggcgcccggc
     5341 gtggccggca tgctgcgcaa gcagggtttc gcggaggcat tcgcgttgcg cggcggcatc
     5401 gcggcttggc agcaggcgaa cctgccgctg gaaaaaggct agaactcccc cgatggccag
     5461 gatcacgatg tacagcacgg gcgtctgccc gttctgccag atggccgagc ggctgctgcg
     5521 ctcgaagggc gtttccgtca
//
LOCUS       DBNGSG010000243         9105 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2794177, whole genome shotgun sequence.
ACCESSION   DBNGSG010000243 DBNGSG010000000
VERSION     DBNGSG010000243.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9105)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9105)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9105
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2794177"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="AC158Q_06415"
     CDS             complement(<1..244)
                     /locus_tag="AC158Q_06415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HIR3339443.1"
                     /translation="MKVLVTVKRVIDPQVKVRVKSDGSGVETANIKMAMNPFCEIAVE
                     QAVRMKEAGVVTEIVAVSIGAPQCQETLRTAMAMGAD"
     gene            complement(343..1233)
                     /locus_tag="AC158Q_06420"
     CDS             complement(343..1233)
                     /locus_tag="AC158Q_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR3339444.1"
                     /translation="MVAFFLAAPAVIYPVFLMKVMCFALFASAFNLLLGYVGLLSFGH
                     AMFLGMAGYTAAHAAKDWGLTPELAILAGAAASSVLGLLSGLIAIRRQGIYFAMITLA
                     LAQMIYFFCVQAPFTHGEDGIQAVPRGRLFGFIDLSDVRAMYATVFVIFTAGFLLIYR
                     VIHSPFGQVLKAIRENETRATSLGYDTDRYKLLAFVLSAGLSGLAGATNAIVFQLAAL
                     NAVHWTMSGEVVLMTLVGGLGTVFGPVAGALVVLAMENYLAQLGAWVTIAQGVIFVVC
                     VLTFRRGIIGELGRLLKKPL"
     gene            complement(1323..2210)
                     /locus_tag="AC158Q_06425"
     CDS             complement(1323..2210)
                     /locus_tag="AC158Q_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR3339445.1"
                     /translation="MSEIFGVPTQALFGQLLIGLINGSFYALLSLGLAVIFGLLNIIN
                     FAHGAQYMMGAFCAWFLLNKLGLNYWWALVITPVAVGATGMAIERLMISRLYQLDHLY
                     GLLLTFGLALIIQGLFRQEFGSTGLPYPMPGALEGGQNLGFMFLPNYRAWVIVASIAV
                     CLGTWFMIEKTKLGSYLRAATENPQLTQAFGINVPRMITLTYGFGVGLAGLAGVMAAP
                     IYSVNPLMGEHLIIVVFAVVVIGGMGSIMGSIITGFVLGLVEGFTKVFYPEASNTVIF
                     IIMAIVLMIRPAGLFGAQK"
     gene            complement(2306..3514)
                     /locus_tag="AC158Q_06430"
     CDS             complement(2306..3514)
                     /locus_tag="AC158Q_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3339446.1"
                     /translation="MTRKHAIGIAAALALAAGAAHAQISDGVIKIGVMNDQSGTYADL
                     AGPGSVVAARMAVEDFGAAKKGMKVEILVADHQNKPEVGSSIARQWYDVDKVDVIVDV
                     PTSSVVLAVNQVTREKNRVLIVSTGATVDLTGKACTPNSVHWTYDTWSLANGTGTAVV
                     KGGGNTWFFLTADYAFGHSLESQTEAVVKKLGGKVNGKVRHPFPSADMSSFLLQAQSS
                     KAKIIGLANAGADTINAIKQGAEFGIVKGGQQFAGLLVFLTDVHSLGLNIAQGLLLTE
                     SFYWDLNDQTRGFSKRFAKRHKGAMPTMGQAGVYSGVLHYLKAVEALKSDQGDKVVAR
                     MKQLPTDDPVFGKGRVRQDGRKIHNMYLFEVKKPSESKGAWDYYKLRGTIPAEQAFRP
                     EKEGGCPLVK"
     gene            complement(3544..4248)
                     /locus_tag="AC158Q_06435"
     CDS             complement(3544..4248)
                     /locus_tag="AC158Q_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3339447.1"
                     /translation="MSEALLTVKGLNAWYGESHILHGVDFEVHAGEVVTLLGRNGAGK
                     TTTLKSVMGMVGRRQGSIAYEGKETIGLASNRIARMGIAFCPEERGIFASLDVQENLL
                     LPPRVREGGFSLRQVYEIFPNLEERSASQGTKLSGGEQQMLAIGRILRTGARLLLLDE
                     PTEGLAPVIVQQIGRTIARLKQEGFTILLVEQNFRFAQAVADRHYVMENGRVVDMIPN
                     AGLDAGMAKIQEYLGV"
     gene            complement(4273..5028)
                     /locus_tag="AC158Q_06440"
     CDS             complement(4273..5028)
                     /locus_tag="AC158Q_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3339448.1"
                     /translation="MSASEIILETRGLTKEFKGFIAVNGVNLKVRRGSIHALIGPNGA
                     GKTTCFNLLTHFLDPTRGSIFFNGREITGSKPSSIARMGLVRSFQISAVFPHLTVIEN
                     VRIALQRRRGASFDFWRSERVLDALNARARELIEAVGLSEFEGAVAVELPYGRKRALE
                     IATTLALDPEMMLLDEPTAGMTHADVERITALIKRVAANRTVLMVEHNLSVVSNLSDR
                     ITVLQRGEVLAEGDYATVSKDPGVVEAYLGKGH"
     gene            complement(5084..6457)
                     /gene="radA"
                     /locus_tag="AC158Q_06445"
     CDS             complement(5084..6457)
                     /gene="radA"
                     /locus_tag="AC158Q_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HIR3339449.1"
                     /translation="MAKAKSLYACTECGGQVLKWQGQCPHCQAWNTLVETVAEAVPAG
                     TNRYGRIAEAGKPQRLSEVEAREESRIPSGVEEFDRVLGGGLVPGGVVLIGGEPGIGK
                     STLLLQSLAEIGKTRKVLYVTGEESPQQIALRARRLGVDAGNVIIFPDTDLGRIESVL
                     KSEQPQVAVIDSIQTLYFAELQSAPGSVAQVRECAARLTRVAKSAETTMVFIGHVTKE
                     GALAGPRVLEHMVYTVLYFDGDTHSRYRLIRAFKNSYGAVNELGVFSMEEKGLRGVSN
                     PSALFLSQHDADVAGSCVMVTQEGTRPMLVEIQALVDEAHAPNPRRLSVGLEQNRLAL
                     LLAVLHRHAGIACFDQDVFINAVGGVRITEPAADLAVLMAVVSSLKNRPLPAKLVVFG
                     EVGLAGEVRPVQRGQERLREAAKLGFTHALIPHANRPRQAVAGIEVIAVRRVEEAVVK
                     MRDLVSR"
     gene            complement(6486..7940)
                     /locus_tag="AC158Q_06450"
     CDS             complement(6486..7940)
                     /locus_tag="AC158Q_06450"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HIR3339450.1"
                     /translation="MTRRPTPLQRYAVAAGAVALAFALRYGIYGTLDHRLPFSFFTTA
                     TLVAAWYGGLGPGMFAAICGLLLGDYFYLPAPEAGTTMSEAERTAIGSYALTTTLIVL
                     LFWNLHSRLRDLEDKLGKPRDGAREGTAASGLLAWVEAVDARQPPCVNSLTGFSQRKG
                     GCMRQALRAAAAFAALCLAPAAWAQQGYPSKPIRMIMPWNPGGTTDVTARILGQKMNE
                     TWGQPVVVDARPGASGIIGTEIAMRAPADGYSLLHANMSQWATAPSLYKTAYDIQRDF
                     VPISLVATAPQLLVVPLGLKATSVRELVQLAKSEPGKLNFGSGGAATLAYMGGELLKK
                     LAGVNIVHVPYKGTIFAVTDVMGGRMHIVFSDMPIALPHARSGKLRALAVTSAKRTPL
                     VSDMPTMVESGLPGYVIENSWGVIAPKGVPAPIIAKLNAEIVRVHNLPDVKERYAALG
                     LDAVSSTPERFAQIIRTDASQYSKLIKETGAKAN"
     gene            complement(8029..9096)
                     /gene="alr"
                     /locus_tag="AC158Q_06455"
     CDS             complement(8029..9096)
                     /gene="alr"
                     /locus_tag="AC158Q_06455"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR3339451.1"
                     /translation="MPRPIRATIRASALRHNLKRARDAAGGAQVWAVVKANAYGHGLE
                     RAAQALAEADGFALLDFQEAARLRLAGVTKPILMLEGFFRPLDVNLLFKYGLTPVIHN
                     TEQVELLKHLDFPAELDLYFKVNSGMNRLGFGIDDLRVNYNALRLLPKVRHLTLMTHF
                     ADAEGLEGVHGQLRWFNELVGGIEAPRSLANSAALLRFKEAREGWVRPGIMLYGCSPF
                     AGRTGIESGLRPAMTLESRLITIQALKPGDTVGYGGMFRAERAMRIGVVACGYADGYP
                     RHAPTGTPVLVDGKLTRTVGRVSMDMLCVDLTGMSETGVGARVVLWGEGMPVERVAEA
                     AGTVGYQLLCALAPRVRIVER"
BASE COUNT         1579 a         3098 c         2904 g         1524 t
ORIGIN      
        1 ggtcggcgcc catcgccatc gcggtgcgca gcgtctcctg gcactgcggc gcgccgatcg
       61 agaccgcgac gatctcggtg acgaccccgg cctccttcat gcgcaccgcc tgctcgacag
      121 cgatctcgca gaaaggattc atcgccatct tgatgttggc ggtctcgact ccgctgccgt
      181 cggatttcac cctgaccttg acctgcggat cgatcacgcg cttgacggta accaggacct
      241 tcatgaatgc gctccggaat tgccgcgaaa cgggggtgga agggccgcca ttttaccgca
      301 tagcagcggc ccgataaacg gcggcccaca ctatgggcat cgctacaacg gctttttaag
      361 gagccgtccc agctcgccga taatcccgcg ccggaaagtc agcacgcaga cgacgaagat
      421 cacgccttgc gcgatcgtga cccaggcgcc gagctgcgcg aggtagttct ccatggcaag
      481 caccaccagc gctcccgcga ccggcccgaa caccgtgccc aggcccccga ccagcgtcat
      541 cagcacgacc tcgcccgaca tcgtccagtg caccgcattc aacgccgcga gctggaacac
      601 gatcgcattg gtggcgccgg cgagcccgga caggcccgcc gacagcacga aggcgagcag
      661 cttgtagcgg tcggtgtcgt agccgagcga agttgcgcgc gtctcgttct cgcggatcgc
      721 cttcagcacc tggccgaaag gggaatggat gacgcgatag atcaggagaa agccggcggt
      781 gaagatcacg aacaccgtcg catacatcgc cctcacgtcg gagagatcga tgaagccgaa
      841 gagccggccg cgcggcaccg cctggatgcc atcctcgccg tgcgtgaacg gggcctgcac
      901 gcagaaaaaa tagatcatct gcgccagcgc cagcgtgatc atcgcgaaat aaataccctg
      961 ccgccggatg gcgatcaacc cggacagcag gcccagcacg ctggaagcag cggcgccggc
     1021 gaggatggca agctcgggcg tcaggcccca gtccttggcg gcgtgcgccg cggtgtagcc
     1081 ggccatgccg agaaacatgg catggccgaa ggacagcagc ccgacgtaac ccagcagcag
     1141 gttgaaggcg ctcgcgaaga gggcaaaaca catcaccttc atcaggaaca ccgggtagat
     1201 cacggccggc gccgcgagga agaaggccac catcgcgagg aaagcgatgc gctggtgcgc
     1261 gggcacgcgg ctccaccacc aacccgtcgc atgccgcgtg gaggccgctt ccatcgcccg
     1321 ccctatttct gcgcgccgaa caggccggcc ggccggatca tgagcacgat cgccatgatg
     1381 atgaagatga ccgtgttcga ggcctcggga tagaacacct tggtgaagcc ctcgaccagc
     1441 cccagcacga agccggtgat gatcgagccc atgatcgagc ccatgccgcc gatcaccacc
     1501 accgcgaata cgacgatgat gagatgctcg cccatcaggg gattcacgct gtagatcggc
     1561 gcggccatca cgcccgcgag ccctgcaagc ccgacgccga agccgtaggt gagcgtgatc
     1621 atccgcggca cgttgatgcc gaacgcctgc gtcaactgcg gattttcggt cgcggcgcgc
     1681 aggtacgacc cgagcttggt cttctcgatc atgaaccagg tgccgaggca taccgcgatc
     1741 gaagcgacga tcacccaggc gcggtagttc ggcaggaaca tgaaacccag gttctgcccg
     1801 ccctccagcg ccccgggcat ggggtacggg agccccgtgg agccgaactc ctgccggaac
     1861 agcccctgga tgatgagcgc cagcccgaaa gtcagcagca gcccgtagag atggtccagc
     1921 tggtacagcc gcgagatcat cagccgctcg atcgccatgc cggtcgcgcc caccgcgacg
     1981 ggcgtgatga cgagcgccca ccaatagttg aggccgagct tgttcaacag gaaccacgcg
     2041 cagaaggccc ccatcatgta ctgggcgccg tgcgcgaagt tgatgatgtt gagcagcccg
     2101 aagatgacgg caagcccgag cgacaacagc gcgtagaacg agccgttgat cagcccgatc
     2161 aggagctggc cgaacagcgc ctgggtggga acgccgaaga tctcggacat caggattctg
     2221 gaatcaggag tcaggattca ggaaaacagc aatgcggctc tcgcattcac tgaatcccga
     2281 atcccggatc ccggatcctg cctttctact tgaccagcgg gcagccgcct tccttctccg
     2341 gccggaacgc ctgctcggcg gggatggtgc cgcgcagctt gtagtagtcc cacgcgccct
     2401 tcgattccga aggcttcttc acctcgaaca ggtacatgtt gtggatcttg cggccgtcct
     2461 ggcgcacgcg cccctttccg aacaccgggt cgtcggtcgg cagctgcttc atcctggcga
     2521 ccaccttgtc gccctggtcc gacttcagcg cctcgaccgc cttcaggtaa tgcagcacgc
     2581 cggaatagac gcccgcctgt cccatggtcg gcatcgcgcc cttgtggcgc ttggcgaagc
     2641 gctttgaaaa gccgcgcgtc tggtcattga gatcccagta gaacgactcg gtgagcagca
     2701 ggccctgcgc gatgttcaac ccgagcgaat ggacgtcggt caggaacacg agcaggccgg
     2761 caaactgctg gccgcccttc acgatgccga actccgcgcc ctgcttgatc gcgttgatgg
     2821 tgtccgcccc ggcgttcgcc agcccgatga tcttggcctt ggacgactgc gcctgcagca
     2881 ggaacgagga catgtcggcc gacgggaacg gatggcgcac ttttccgttc accttgccgc
     2941 cgagtttctt cacgaccgcc tcggtctggc tctcgagcga gtggccgaac gcatagtcgg
     3001 cagtcaggaa gaaccaggta ttgccgccgc ccttgaccac cgccgttccg gtgccgttgg
     3061 cgagcgacca ggtgtcgtag gtccagtgga ccgagttcgg ggtgcatgcc ttccccgtca
     3121 ggtccaccgt ggcgccggtg gagacgatga gcaccctgtt cttctcgcgc gtcacctggt
     3181 tgaccgccag cacgaccgag gaagtcggca cgtcgacgat cacgtccacc ttgtccacgt
     3241 cgtaccactg gcgcgcgatg ctcgacccga cctccggctt gttctggtga tcggcgacga
     3301 gaatttcgac cttcatgccc ttctttgccg cgccgaaatc ctccaccgcc atgcgcgcgg
     3361 cgacaaccga acccggcccg gcgaggtcgg cgtaggtgcc cgactggtcg ttcatcacgc
     3421 cgatcttgat cacgccatcg gaaatctggg cgtgcgcggc tccggcggcg agcgcgagtg
     3481 ctgcggcgat gcctattgcg tgttttcttg tcatgagggt tctcctgttg cgggttgcac
     3541 gaattagacg ccaaggtatt cctgtatctt cgccatgccg gcatccagcc cggcgttggg
     3601 gatcatgtcc acgacgcggc cgttctccat cacgtaatga cggtcggcaa cggcctgggc
     3661 aaagcggaag ttctgttcca cgagcaggat ggtaaaccct tcctgcttga gacgcgcgat
     3721 ggtgcgcccg atctgctgca cgatgaccgg cgcgaggccc tcggtcggct cgtcgagcag
     3781 cagcagccgc gcgccggtgc gcaggatgcg cccgatcgcg agcatctgct gctctccgcc
     3841 ggagagcttg gtgccctggc tcgcggagcg ctcctccagg ttgggaaaga tctcgtatac
     3901 ctgccgcagg ctgaaaccgc cctcgcgcac cctgggaggc agcagcaggt tctcctgcac
     3961 gtcgaggctc gcgaaaatcc cgcgctcctc ggggcagaag gcgatgccca tgcgcgcgat
     4021 gcggttggag gcgagcccga tcgtctcctt tccctcgtag gcaatcgagc cctggcgccg
     4081 gccgaccatg cccatgaccg acttgagggt cgtggtcttg ccggcgccgt tgcgcccgag
     4141 cagcgtcacc acctcgccgg catgcacctc gaaatccacg ccatgcagga tgtgcgattc
     4201 cccgtaccag gcgttcaggc ccttgactgt aagcaatgcc tcactcatca ctcatcattc
     4261 atcgttcatc attcaatgcc cctttcccag gtacgcctcg acgacgccgg ggtccttcga
     4321 cacggtcgcg taatcgccct cggccaggac ctctccccgc tgcagcacgg tgatccggtc
     4381 cgacaggttg gagacgacgg aaagattgtg ctcgaccatc agcaccgtgc ggttggcggc
     4441 aacgcgcttg atgagggccg ttatgcgctc gacatccgcg tgcgtcatgc ccgcggtcgg
     4501 ctcgtcgagc agcatcattt ccgggtcgag cgcgagcgtg gtggcaatct cgagcgcccg
     4561 cttgcggccg tacggcagct cgaccgccac cgcgccctcg aactcggaca gtcccacggc
     4621 ctcgatcagt tcgcgcgcgc gggcgttcag cgcatcgagc actcgctcgg agcgccagaa
     4681 atcgaacgac gctccgcgcc tcctttgcag cgcgatgcgc acgttctcga tcacggtcag
     4741 gtgcgggaac accgccgaga tctggaacga gcgcacgaga cccatgcggg cgatgctcga
     4801 gggcttggag ccggtgatct cgcgcccgtt gaagaagatg ctgccgcgcg tgggatccag
     4861 aaaatgcgtg agcaggttga agcacgtggt cttgccggcg ccgttggggc cgatcagcgc
     4921 gtggatgctg ccgcgccgca ccttgaggtt cacgccattc accgcgatga agcccttgaa
     4981 ctccttggtg agcccgcggg tctcgaggat gatttcgctc gcgctcatac ggaatgcgtg
     5041 acgggtgacg aatgacggtt gacggccgga aacgctgcca gcgtcatctt gagaccaggt
     5101 cgcgcatctt cacgacggct tcctcgacgc gccgcaccgc gatcacttcg atgcccgcga
     5161 ccgcctgcct gggacggttg gcatgcggga tcagcgcgtg ggtaaagccg agcttggccg
     5221 cttcgcgcag gcgctcctgc ccgcgctgca ccgggcgcac ctcgccggca aggcccacct
     5281 caccgaacac gacgagcttg gcgggcagcg gcctgttttt cagcgacgac accaccgcca
     5341 tcagcaccgc gaggtcggcg gcgggctcgg tgattcttac cccgccgacg gcgttgatga
     5401 acacgtcctg gtcgaagcag gcgatgccgg cgtgccggtg cagcaccgca agcagcagcg
     5461 cgagccggtt ctgctcaagc cccaccgaaa ggcggcgcgg gttgggcgcg tgcgcctcgt
     5521 cgaccagcgc ctggatctcg accagcatcg ggcgcgtgcc ttcctgcgtc accatcacgc
     5581 acgagccggc gacatcggca tcgtgctgcg acaggaacag cgccgaaggg ttggatacgc
     5641 ctctcagccc cttctcctcc atggagaaca cgcccagctc gttgaccgcc ccgtagctgt
     5701 tcttgaaggc gcggatcagc ctgtagcgcg agtgcgtgtc gccgtcgaaa tacagcaccg
     5761 tgtacaccat gtgctcgagc acgcgcgggc cggccagcgc gccctccttg gtgacgtgcc
     5821 cgatgaacac catggtggtc tcggcgctct tggcgacccg ggtcagccgg gcggcgcatt
     5881 cgcgcacctg cgccaccgac cccggcgccg actgcagctc ggcgaaatac agggtttgta
     5941 tggagtcgat gaccgcgact tgcggctgct ccgacttcag caccgattcg atcctgccga
     6001 ggtcggtgtc cggaaagatg atcacgttgc ccgcgtccac gcccaggcgc cgcgcgcgca
     6061 gcgcgatctg ctgtggcgat tcctcgccgg tcacgtagag caccttgcgc gtttttccga
     6121 tctccgccag cgactgcagc agcagcgtgg acttgccgat gccgggctcg ccgccgatca
     6181 gcaccacgcc gccgggcacc agcccgccgc ccagcacgcg gtcgaattcc tcgacgccgc
     6241 tgggaatgcg ggactcctcc cgcgcctcga cctcggagag ccgctgcggc ttgcccgcct
     6301 cggcgatgcg cccgtagcgg ttggttcccg cgggcacggc ctcggcgacg gtttcgacca
     6361 gcgtgttcca ggcctggcag tgggggcact ggccctgcca cttcagcacc tggccgccgc
     6421 attcggtgca ggcgtagagc gatttcgcct tggccatgga aaacgtcaga aaacgggcgg
     6481 cgccatcaat tcgccttcgc cccggtctcc ttgatgagct tcgaatactg gctcgcgtcc
     6541 gtgcggatga tctgcgcgaa tcgctccggc gtgctgctga cagcgtccag acccagcgcc
     6601 gcatagcgct ccttgacgtc cggcaggttg tgcacccgca cgatctcggc gttgagtttg
     6661 gcgatgatgg gagccggcac ccctttcggc gcgatgacgc cccaggagtt ctcgataacg
     6721 tatcccggca acccggattc caccatcgtc ggcatgtcgg acacgagcgg cgtgcgcttt
     6781 gcgctggtga ccgcaagcgc gcgcagcttg ccggaccggg catggggcag cgcgatcggc
     6841 atgtcggaga agacaatgtg catccggccg cccatcacgt cggtgaccgc gaagatcgtg
     6901 cccttgtacg gaacatgcac gatgttgacg cccgcaagct tcttgagcag ctcgccgccc
     6961 atgtaggcaa gcgtcgccgc gccccccgat ccgaagttga gctttcccgg ctcggacttc
     7021 gcgagctgga cgagttcgcg caccgacgtc gccttgagcc ccagcgggac taccagcaac
     7081 tgtggcgcgg tggcaaccag gctgatcggc acgaaatcgc gctggatgtc gtaagcggtc
     7141 ttgtacaggc tgggggccgt cgcccactgg ctcatattcg cgtgcaacag cgagtagccg
     7201 tcggcgggtg cccgcatcgc gatctcggtg ccgatgatgc cgctcgcgcc ggggcgtgcg
     7261 tcgacaacga cgggttggcc ccaggtctcg ttcatcttct ggcccagtat cctcgcggtc
     7321 acgtccgtgg tcccgcccgg gttccacggc atgatcatgc gtatcggttt gctgggatag
     7381 ccctgctgcg cccaagctgc gggagcgagg cagagtgccg cgaacgctgc ggcggctcga
     7441 agtgcttgtc gcatacatcc cccttttcgt tgactgaacc cggtcaagct attaacgcat
     7501 ggcggctgtc gtgcgtctac cgcctcaacc cacgccagca accccgacgc ggcagttccc
     7561 tcgcgcgcgc cgtcccgcgg cttgccgagc ttgtcctcga gatcgcgcag gcgcgagtgc
     7621 aggttccaga acagcagcac gatcagcgtg gtggtcagcg cgtagctgcc gatcgcggtg
     7681 cgctcggcct cgctcatcgt ggtgccggcc tcaggtgccg gcaggtaaaa gtaatcgccc
     7741 agcaacaggc cgcaaatcgc ggcgaacatg ccggggccga ggccgccgta ccacgccgcg
     7801 accagcgtcg ccgtggtgaa gaaggaaaac ggcagccggt ggtcgagcgt gccgtagatg
     7861 ccgtagcgca gcgcgaacgc cagcgccacc gcgccggcgg cgacggcata tctctggagg
     7921 ggggttggcc tgcgcgtcat tgcgatggtc taagagtccc taaccgagcg ggcgaatcat
     7981 catacaccgc gcgatacggc atgacagtga gtcgtggcgc tggcggcgtc atctttccac
     8041 gatgcgaacg cgcggcgcga gggcgcatag cagctgatag cccaccgtcc ccgccgcctc
     8101 tgcgacccgc tccaccggca tgccttctcc ccacagcacg acgcgagccc ccacgccggt
     8161 ttctgacatt ccagtcaggt cgacacacag catgtccatg gacacccggc cgacggtgcg
     8221 ggtgagcttg ccgtccacga ggacgggagt cccggtcggc gcgtgccgcg ggtagccatc
     8281 ggcataaccg caggcgacga ccccgatccg catcgcgcgt tcggcccgga acatcccgcc
     8341 gtagcccacg gtgtcgccgg gcttcagcgc ctggatcgtg atgagacggc tctcgagcgt
     8401 catggccggc cgcagtcccg attcgatacc cgtcctgccg gcaaacggcg agcagccgta
     8461 gagcatgatg ccgggccgca cccagccctc gcgcgcctcc ttgaagcgca gcagcgccgc
     8521 cgagttggcg agcgacctcg gcgcctcgat gccgcccacc agctcgttga accagcgcag
     8581 ctggccgtgg acgccttcca gcccttcggc gtcggcgaag tgcgtcatca gcgtgaggtg
     8641 gcgcaccttc ggcagcagcc gcagggcgtt gtagttgacg cgcaggtcgt cgatcccgaa
     8701 gcccagccgg ttcatgccgc tgttcacctt gaagtacagg tccagctcgg ccggaaaatc
     8761 caggtgcttg agcagctcta cctgctcggt gttgtggatg accggggtga gcccgtactt
     8821 gaacagcagg ttgacgtcca acggccggaa gaaaccttcc agcatgagga tgggcttggt
     8881 gacccccgcc agccgcagcc gcgcggcctc ctggaagtcg agcagcgcga agccgtcggc
     8941 ctcggcgagg gcctgggccg cgcgctccag gccgtggccg taggcgttcg ccttcaccac
     9001 ggcccagacc tgcgcgccgc cggccgcgtc gcgcgcccgc ttcaggttgt ggcgcagcgc
     9061 ggacgcgcgg atcgtggcgc ggatcggccg aggcatggcg ctagc
//
LOCUS       DBNGSG010000244         3225 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_757212, whole genome shotgun sequence.
ACCESSION   DBNGSG010000244 DBNGSG010000000
VERSION     DBNGSG010000244.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_757212"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="AC158Q_06460"
     CDS             complement(<1..733)
                     /locus_tag="AC158Q_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006402717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HIR3339452.1"
                     /translation="MDSVDLQVLKSARDWLQAGRRVVMATVIRTWGSAPRPIGALTAI
                     RDDGMVVGSVSGGCIEDDMILQVRSGELVKDKPATTQYGVSAEEAKRFGLPCGGTLEL
                     VLEPLTKESAIDELLARVERHELVKRQLDMKTGRAIVGPAQSNDQLAFDGATLSTVHG
                     PRWRLLIIGAGQLSSFLAQMAQGLDYQVTICDPREEYADGWNVAGVEFKRGMPDDVVL
                     GMNADGHTAVVTLTHDPKLDDMALLE"
     gene            952..1395
                     /locus_tag="AC158Q_06465"
     CDS             952..1395
                     /locus_tag="AC158Q_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3339453.1"
                     /translation="MEMTGEQLIALPQQATWNALNDPDVLKDCIPGCESITRLSDNEY
                     EVVLTAKVGPVSAKFKGKMTVTDADPPRAYTLSFEGQGGVAGFAKGESTVTLVPEGNG
                     TRLSYIAKATVGGKLAQVGARLIDGVAKQLAGKFFEAFNRRASAG"
     gene            1446..2273
                     /locus_tag="AC158Q_06470"
     CDS             1446..2273
                     /locus_tag="AC158Q_06470"
                     /inference="COORDINATES: protein motif:HMM:NF024118.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3339454.1"
                     /translation="MARLKFFGVRGSISVSGQEYQEFGGNTTCVLMEGPKRTVILDAG
                     TGIRELGKEMAKDPHIGIDRQCFLAFSHFHWDHIQGLPFFTPAYDPRRTFTISAIGRD
                     RYGKDLRSIFETQMQQEYFPVNFDGMGATINFLQTPEDNLRVENAAVKVVKHNHPGGA
                     YSYRFQDSEGKVVVFCTDIEHGERIDPQIVDLARDADVLIHEGQYTPEELASHRGWGH
                     SSWAQAVEVAELARVKRLVVTHHDPDHDDDFLLEIERLCQTRFRDTVLAREKMEIVV"
     gene            2347..2967
                     /locus_tag="AC158Q_06475"
     CDS             2347..2967
                     /locus_tag="AC158Q_06475"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR3339455.1"
                     /translation="MLKIWGRTNSINVQKVLWCCAEMNLQYERVDAGGQFGVNNTPEY
                     LAMNPNGLVPTISDEDFILWESHAIVRYLSRKHGLGTLWPKDDKAAADADRWMEWYST
                     TLWNNLRPIFWNLVRVEAGKRDMKAVEEGRSKMAGNLKMVESRLADRPFMAGNALTAG
                     DIPMGVAVHRWFTLPVERPDYPRLAAYYERLKQRPGYAKHCAVPLT"
BASE COUNT          616 a         1043 c         1032 g          534 t
ORIGIN      
        1 cctccagcag cgccatatcg tcgagcttgg ggtcgtgcgt gagcgtgacc acggcggtgt
       61 ggccgtccgc gttcatgccg agaaccacgt cgtcgggcat gccgcgcttg aattccacgc
      121 cggcgacgtt ccagccatcc gcatattcct cgcgcggatc gcagatggtc acctggtaat
      181 cgagcccctg cgccatctgc gcgagaaaac tcgagagctg ccccgccccg atgatcagca
      241 gccgccagcg cggcccgtgg accgtggaaa gagtcgctcc gtcgaatgcc agctggtcgt
      301 tggactgggc gggcccgacg atcgcgcggc cggtcttcat gtcgagctgc cgcttcacca
      361 gctcgtggcg ctcgacgcgc gccagcagct cgtcgatagc gctctccttc gtgagcggct
      421 cgagcaccag ctcgagcgtg ccgccgcacg gcagcccgaa gcgcttggct tcctccgcgc
      481 tgacgccgta ctgcgtggtc gcgggcttgt ccttcaccag ctcgcccgag cgcacctgca
      541 ggatcatgtc gtcctcgatg cagccgcccg aaaccgagcc caccaccatg ccgtcgtcgc
      601 ggatcgcggt aagcgccccg atcgggcgcg gcgcggaccc ccacgtgcgg atcacggtcg
      661 ccatcaccac gcggcggccg gcctgcagcc agtcgcgggc gcttttcaat acctgcaggt
      721 caacactgtc catgtccggt ttccgatcgc ttctggcaag ggttgtattt taccgaatat
      781 aacggtgaag cgcacgcacc agcggaaacc ggaaacaagc ctcgtgccgc ggatcgggcc
      841 cctgcctctg cgtgctcgaa gtcgggttgc gagcgcggtg aacgtgggct agcatccgcc
      901 cctacgccgc ctgatcgggc ccgcggccac atcaccgaga gcaaacctcc catggaaatg
      961 accggagagc agctgatcgc gctgccgcaa caggccacct ggaacgcgct gaacgacccc
     1021 gatgtgctca aggattgcat cccgggctgc gagtcgatca cccgcctctc ggacaacgaa
     1081 tacgaggtgg tcctgaccgc gaaagtcggc ccggtcagcg ccaagttcaa gggcaaaatg
     1141 accgtgaccg acgcggaccc gccgcgcgcc tacacgctct ccttcgaagg ccagggcggc
     1201 gttgcaggtt tcgccaaggg cgagtcgacc gtcactctcg tgccggaagg caacggcacg
     1261 cgcctttctt acatcgccaa ggcgacggtc ggcggcaagc tcgcgcaggt cggcgcgcgg
     1321 ctgatcgacg gcgtggcgaa gcagctcgcg ggcaagttct tcgaggcttt caaccgccgc
     1381 gccagcgcgg gttgatgcca gcggccggac cggaccaggc gttcgcggaa gcaccaggag
     1441 gaaacatggc ccgcttgaag ttcttcggcg tcaggggttc gatctccgtg tccggccagg
     1501 aataccagga attcggcggc aataccacct gcgtcctgat ggaagggccg aaacgcacgg
     1561 tcatcctcga tgccggcacc gggatccgcg agctgggcaa ggagatggcg aaggacccac
     1621 atatcggaat cgaccggcaa tgcttcctgg cgttctcgca cttccactgg gaccacatcc
     1681 aggggctgcc gtttttcacc cccgcctacg atccgcggcg gacgttcacc atcagcgcca
     1741 tcggccggga tcgttacggc aaggatttgc gcagcatctt cgaaacccag atgcagcagg
     1801 aatactttcc ggtcaacttc gacggcatgg gggcgaccat caacttcctg cagaccccgg
     1861 aagacaacct gagggtggag aacgcggccg tcaaggtcgt caagcacaat caccccgggg
     1921 gcgcctatag ctaccgtttc caggactcgg aaggcaaggt ggtggtgttt tgcaccgaca
     1981 tcgagcacgg cgagcgcatc gatccgcaaa tcgtcgatct ggcccgtgac gcggacgtat
     2041 tgattcacga gggccagtac acgccggaag aactggcgag ccaccggggc tggggccaca
     2101 gcagctgggc gcaggcggtg gaagtcgctg aactggcccg ggtcaagcgg ctcgtcgtta
     2161 cgcaccacga ccccgatcat gacgacgact tcctgctcga gatcgagagg ctgtgtcaga
     2221 cccgttttcg ggacaccgtg ctggcgcggg aaaaaatgga aatcgtggtc tgagccccgc
     2281 gcgcgccggc ggtttgccgg gccgaaccac gattcacgga tcacgcattt gaaaccggag
     2341 gtcaacgtgt tgaaaatctg gggccgcacc aattccatca acgtgcagaa ggtgctgtgg
     2401 tgctgcgcgg aaatgaatct tcagtacgag cgcgtggacg cgggcggaca gttcggcgtc
     2461 aataacacgc ctgagtacct ggccatgaat cccaacgggc tggtgccgac gatcagtgat
     2521 gaggacttca tcctgtggga atcgcacgcc atcgtgcgct acctgtcgcg caagcacggg
     2581 ctgggcacgc tgtggccgaa ggacgacaag gcggccgccg acgccgaccg ctggatggag
     2641 tggtacagca cgacgttgtg gaacaacctg cgccccattt tctggaacct ggtgcgcgtc
     2701 gaggcgggca agcgcgacat gaaggcggta gaggaaggcc gctcgaagat ggccggaaat
     2761 ctcaagatgg tggaaagcag actggcggac cgcccgttca tggcgggcaa cgccctcacc
     2821 gcgggcgaca tcccgatggg cgtggcggtg caccgctggt tcaccctgcc cgtcgagcga
     2881 ccggattacc cgcggctggc ggcgtactac gagcgactca agcagcgtcc cggctacgcg
     2941 aagcactgcg cagtcccgct tacttaagcg taagcgggac ccaatagata cagagcctga
     3001 atcgtgccgg cggtcggatg aggcgcggcc gatgatcctg gtgtcggctg cattcacact
     3061 ccctcctgat tgcgatatcc actgcaaaag atgacgcaag tccgcgccta ccgctactac
     3121 gaattcgtga tggcagcgtt cgtcacggtg ctgatctgct ccaacctgat cgggccggcg
     3181 aagatcgcgc agctcgaact gccggtcatc ggcacgttcg tgatg
//
LOCUS       DBNGSG010000245         1718 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4153294, whole genome shotgun sequence.
ACCESSION   DBNGSG010000245 DBNGSG010000000
VERSION     DBNGSG010000245.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4153294"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..1196)
                     /locus_tag="AC158Q_06480"
     CDS             complement(195..1196)
                     /locus_tag="AC158Q_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3339456.1"
                     /translation="MSKRHISILSVLVLATLGAAPGAWAWEPTKSVEFVIPAGTGGGA
                     DQMARFTQGVTAKHGLMKQSVIPVNKSGGAGAEGFLDVKASKGDNHKIIITLSNLFTT
                     PLATGVPFNWKDLTPVAMLALDNFVLWVPADTPYKSAGEYLAAVKKAGPNKYKMGGTG
                     AKQEDQIITVAIEKASGTKFTYVPFKGGGAVATQLVGKHVDSTVNNPIEAVAHWRSGK
                     LRPLCVFEKARMPYKNKVTATMSWNDIPTCREAGIPMDYLMLRGIFMPAGVSQEVVDF
                     YIGFFRKVRGTAEWNKLMEDGAFNQSFMTGKEYVSWVANAENLHRQLMTEAGFIGRK"
     gene            complement(1401..>1718)
                     /locus_tag="AC158Q_06485"
     CDS             complement(1401..>1718)
                     /locus_tag="AC158Q_06485"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3339457.1"
                     /translation="TPVVYRGVLPVGLTTPEMHARAIERTAARHLGFIEKAARRAGVR
                     CRCVHVTSDFPADAIMAAARRERCDLIVMASRSKRGLSGMLLGSQTHKVLAHSKVPVL
                     VQR"
BASE COUNT          280 a          579 c          530 g          329 t
ORIGIN      
        1 aagaccagca gcgcgacgac gaggtcgacg acaacagtgc gcgccgcccc ctgggtggag
       61 ttctcgttat gcgccattca aagatcgctg ccccgccgga atcgcggcgg cggcgccact
      121 cgggcgccgc cgccgggcaa tgaaatagac gggttgccgg gtcagcaccc gatacccgtc
      181 tgttgtggct gtcgttactt cctgccgatg aatcccgcct cggtcatcag ctgccggtgc
      241 aggttttcgg cattggcaac ccagctcacg tactccttgc ccgtcatgaa cgactggttg
      301 aaggcgccgt cctccatgag cttgttccat tccgcggtgc cgcgcacctt gcggaagaac
      361 ccgatgtaga aatcgaccac ctcctgcgac acgccggccg gcatgaagat gccgcgcagc
      421 atgaggtaat ccatcgggat gcccgcctcc cggcaggtcg ggatgtcgtt ccacgacatg
      481 gtcgcggtca ccttgttctt gtacggcatg cgcgccttct cgaacacgca cagcgggcgc
      541 agcttccccg atcgccagtg cgcgaccgcc tcgatcggat tgttgaccgt ggaatccacg
      601 tgcttgccga cgagctgcgt cgcgaccgcg ccgccgccct tgaacggcac gtaggtaaac
      661 ttggtgccgg aggccttctc gatcgcgacg gtgatgatct ggtcctcctg cttggcgccg
      721 gtgccgccca tcttgtactt gttgggcccg gccttcttga ccgcggcgag gtattccccg
      781 gccgacttgt acggcgtgtc cgcgggcacc cacagcacga aattgtcgag cgcgagcatc
      841 gcgaccggcg tcaggtcctt ccagttgaac ggcacgcccg tggcaagcgg cgtcgtgaag
      901 aggttcgaca gcgtgatgat gatcttgtgg ttgtcgccct tcgacgcctt gacgtcgagg
      961 aagccctcgg cgcccgcgcc gcccgacttg ttgacgggga tgacggactg cttcatcagg
     1021 ccgtgcttgg ccgtcacgcc ctgtgtgaag cgcgccatct ggtcggcgcc gcccccggtg
     1081 cccgccggga tgacgaactc gaccgacttg gtgggctccc acgcccacgc gcccggtgca
     1141 gctccgagcg ttgcaagcac taacacgctg agtatcgaga tatgccgctt ggacatgacg
     1201 tccctccttt tatggtttag ctacaccttc gtcaacctgg ccgacgctcc gcccacaccg
     1261 cgggttccga tgggctcgta atgtgccgga acggacccta actatacttg accgaggtca
     1321 atttcccgaa attcgcggac cgcggcaccc gggccaagca tactgcagtg caacatgcca
     1381 ggcggcctgt cccggcagcc tcagcgctgc accagcaccg gcaccttgga atgagccagt
     1441 actttgtggg tctggctccc gagcagcatc ccggagagcc cgcgcttgct gcgggaagcc
     1501 atcacgatga gatcgcatcg ttcccgcctc gcagcggcca tgatcgcgtc cgcggggaag
     1561 tcactggtga cgtggacgca cctgcagcgt acgccggccc tgcgagccgc tttttcgatg
     1621 aacccgagat gtctggctgc cgtgcgctcg attgcgcggg catgcatctc gggtgtcgtc
     1681 aggccgacgg gcagcacccc tctgtagact accggtgt
//
LOCUS       DBNGSG010000246         3547 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_77474, whole genome shotgun sequence.
ACCESSION   DBNGSG010000246 DBNGSG010000000
VERSION     DBNGSG010000246.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_77474"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1581)
                     /locus_tag="AC158Q_06490"
     CDS             complement(1..1581)
                     /locus_tag="AC158Q_06490"
                     /inference="COORDINATES: protein motif:HMM:NF052759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339458.1"
                     /translation="MSRTKVLSAILVAAVLAAGAGAFWWWGARGAAPDYRLAKVERGS
                     ITAAVSTTGTVNPVTVVQVGSQVSGQLKEVLADFNSAVKKGQVIARIDADSFALRVNQ
                     AMADLEAARATVLTQRANVAALHAEVSRARVGLADAEREFQRNKMLHEKNFVSGAALD
                     KAEFAYRNAQEQVKVAQAQLAVGESQVRNVEALVKQRDSQLSQARVDLERTTIRAPVD
                     GIVVKKSVEPGQTVAASLQAPELFVIAQDLRQMQVDTSIDESEVGRIREGQQATFTVD
                     SFPGRTFRGTVGQVRKAALVVQNVVTYTAVIATSNPGLELFPGMTANVRIVVDSRENA
                     LKVPNAALRCRPAGAAPEARAADESAETAATQEKKKGAAGQQAAAAQRERLTRELNLS
                     TEQQAKLEAILNETRDRIRAIATEEKGERRKQAERLRAESRARIAEMLNPEQRKRYEE
                     MTPGRAGGRRTATSGRVWVLDDSGRPKAVNLRIGLADGTYSEVVGGELKEGMQIIVGT
                     QTDAKSRGQAKGGPRFGF"
     gene            complement(1757..2128)
                     /locus_tag="AC158Q_06495"
     CDS             complement(1757..2128)
                     /locus_tag="AC158Q_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339459.1"
                     /translation="MRLRSMIAAAVLALAFAAPSRAGDRPDPFAQLEPLIASGVLYRG
                     LVREEDVTLLFAHLRAALAASQQGREAPPPPEALDRRAQEIAAEIRARGTLAGLLLLT
                     AFEAAALQALRDAFPPTAPAR"
     gene            complement(2186..>3547)
                     /gene="thiC"
                     /locus_tag="AC158Q_06500"
     CDS             complement(2186..>3547)
                     /gene="thiC"
                     /locus_tag="AC158Q_06500"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HIR3339460.1"
                     /translation="QRQHAGQSFGATLPRAITPEFVRDEVARGRAIIPANINHPETEP
                     MIIGRNFLVKINANIGNSALSSGIQEEVEKMTWAIRWGGDTVMDLSTGKHIHETREWI
                     VRNSPVPIGTVPIYQALEKVDGRAEELTWEIYRDTLIEQCEQGVDYFTVHAGVRLPFI
                     PMTAKRMTGIVSRGGSIMAKWCLAHHKESFLYTHFEEICELLKAYDVSFSLGDGLRPG
                     SIYDANDEAQIAELKTLGELTDIAWKHDVQVMIEGPGHVPMQLIRENMDLQLKYCREA
                     PFYTLGPLTTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPDKDDVKDGIM
                     AYKIAAHAADLAKGHPGAQIRDNALSKARFEFRWEDQFNLGLDPDKAKQFHDETLPQE
                     GAKLAHFCSMCGPHFCSMKITQDVREYAERQGVTGEEALEKGMQVKAKEFVESGAEIY
                     RRT"
BASE COUNT          489 a         1243 c         1156 g          659 t
ORIGIN      
        1 ttagaagccg aagcgcgggc cgcccttggc ctggccccgc gatttcgcat ccgtctgcgt
       61 cccgacgatg atctgcatcc cttccttcag ctcaccgccc accacctcgc tgtaggtgcc
      121 gtcggcaagc ccgatgcgca ggttgacggc cttcggcctg ccgctgtcgt ccagcaccca
      181 gacgcgcccg ctcgtggcgg tgcggcggcc tccggccctt cccggggtca tttcctcgta
      241 acgcttgcgc tgctcgggat tcagcatctc ggcgatgcgc gcgcgggatt ccgcgcgcag
      301 ccgctcggcc tgctttcggc gctcgccctt ttcctcggtc gcaatcgcgc ggatccggtc
      361 gcgggtttcg ttcagaatcg cctccagctt cgcctgctgc tcggtgctga gattcaactc
      421 cctggtgagc cgctcgcgct gcgccgccgc agcctgctgc ccggccgcgc ccttcttctt
      481 ctcttgcgtc gctgccgtct cggccgactc gtcggccgcg cgggcttccg gcgcggcccc
      541 cgccgggcgg cagcgcagcg ccgcattcgg caccttgagg gcgttctcgc ggctgtccac
      601 gacgatgcgc acgttggcgg tcatgccggg gaacagctcg aggccgggat tggaagtggc
      661 gatgaccgcg gtataggtga cgacgttctg caccaccagg gccgctttgc gcacctgccc
      721 caccgtgccg cggaaggtcc tgccgggaaa cgaatccacg gtgaaggtcg cctgctggcc
      781 ctcccggatg cgcccgactt ccgactcgtc gatcgaggtg tcgacctgca tctggcgcag
      841 gtcctgcgcg atcacgaaca gttccggcgc ctgcaggctt gcggcgaccg tttgcccggg
      901 ctccaccgat ttcttgacga cgatgccgtc caccggcgcg cggatggtgg tgcgctcgag
      961 atccactctt gcctgcgaga gctgggaatc gcgctgcttc accagcgctt ccacgttgcg
     1021 cacctgggac tcgcccaccg cgagctgggc ctgcgcgacc ttgacctgtt cctgggcatt
     1081 gcggtaggca aactcggcct tgtcgagcgc cgcccccgaa acgaaattct tctcgtgcag
     1141 catcttgttg cgctggaact cgcgctcggc atcggccagg ccgaccctgg cgcgcgagac
     1201 ctcggcatgc agcgccgcga cgttggcgcg ctgggtgagc accgttgcgc gagccgcttc
     1261 caggtccgcc atcgcctggt tgacgcgcaa cgcgaacgag tccgcgtcga tgcgggcgat
     1321 cacctgccct ttcttgaccg ccgagttgaa gtcggcgagg acttccttca actgcccgga
     1381 gacttgcgag ccgacctgca ccacggtcac cgggtttacc gtgccggtgg tcgagaccgc
     1441 cgccgtgatg ctcccgcgct cgaccttggc gaggcggtag tccggcgccg cgccgcgcgc
     1501 cccccaccac cagaacgcgc cagcgccggc ggccagtacc gcggccacga ggatcgcgct
     1561 caggactttc gtgcgtgaca ttcggctctt ttcgatccgt gacgggagac gcgcaatacg
     1621 ccgcccgcat gggcccgccg ccttccatgg cccatgggcg tcgaccggct atcggcgccg
     1681 gccattgcct tgaatagcgg ggattgtaac ggaggcgccg cagccctccc acggcacggc
     1741 agcgcgggtg tcgatctcag cgcgccggcg cggttggcgg aaaagcgtcc cgcaacgcct
     1801 gtagtgccgc cgcctcgaac gcggtaagga gcaacaaccc cgcgagcgtc ccgcgggccc
     1861 tgatctcggc ggcgatttcc tgcgcgcggc gatcgagcgc ctccggcggc ggcggcgcct
     1921 cgcggccctg ctgggaggcg gcgagcgccg cccggaggtg ggcgaacagc agcgttacgt
     1981 cctcctcgcg gaccagtccc cggtagagca cgcccgaggc gatcaacggc tcgagttggg
     2041 cgaaaggatc gggcctgtcc ccggcccggg aaggggcggc gaatgccagc gccagcacgg
     2101 cggcggcaat catcgaacgc aggcgcatgg cagtctcctc atgggcactc gtgacatcct
     2161 ataacgcgcg attggcgctc ccggtttacg tccgccggta gatttccgcg ccgctctcga
     2221 cgaactcttt cgccttgacc tgcatgcctt tctccagcgc ctcctccccg gtgacgccct
     2281 ggcgttcggc gtactcgcgc acgtcctggg tgatcttcat cgagcagaaa tgcgggccgc
     2341 acatcgagca gaaatgcgcg agtttcgcgc cctcctgcgg cagcgtctcg tcgtgaaact
     2401 gcttcgcctt gtcggggtcg agcccgaggt tgaactggtc ctcccagcgg aactcgaagc
     2461 gcgctttcga cagcgcgttg tcgcggatct gcgcgccggg atggcccttg gcgagatcgg
     2521 ccgcgtgcgc cgcgatcttg taggccatga tgccgtcctt gacgtcgtcc ttgtccggca
     2581 ggcccaggtg ctccttcggc gtcacatagc acagcatcgc ggtgccgaac cagccgatca
     2641 tcgccgcgcc gatcgcgctc gtgatgtggt cgtagcccgg cgcgatgtcg gtggtgagtg
     2701 gccccagcgt gtagaacggc gcctcgcggc aatacttcag ctgcaggtcc atgttctccc
     2761 tgatgagctg catcggcacg tgtcccgggc cttcgatcat cacctgcacg tcgtgcttcc
     2821 aggcgatgtc ggtcaactcg cccagcgtct tcagttccgc gatctgcgcc tcgtcgttgg
     2881 cgtcgtagat cgatccgggg cgcaggccgt cgccgagcga gaacgacacg tcgtaggcct
     2941 tgagcagctc gcagatctcc tcgaagtgcg tgtagaggaa gctttccttg tggtgggcga
     3001 ggcaccactt ggccatgatc gagccgccgc gcgagacgat gccggtcatg cgcttcgcgg
     3061 tcatcgggat gaacggcagg cgcacgccgg cgtgcacggt gaagtaatcc accccctgct
     3121 cgcactgctc gatcagcgtg tcgcggtaga tctcccaggt cagctcctcg gctcgcccgt
     3181 ccaccttctc cagcgcctgg tagatcggca cggtgccgat cggcaccggc gaattgcgta
     3241 cgatccattc gcgagtctcg tggatgtgct tgccggtgga gaggtccatc acggtgtcgc
     3301 cgccccagcg gatcgcccag gtcatcttct cgacctcctc ctggatgccg gaggagagcg
     3361 cggaatttcc gatgttggca ttgattttca ccaggaagtt gcggccgatg atcatcggct
     3421 cggtctccgg gtgattgatg ttggcaggga tgatcgctcg gccgcgcgcg acctcgtcgc
     3481 gcacgaactc gggcgtgatt gcccgcggca gtgtggcgcc gaatgactgc cccgcatgct
     3541 gccgctg
//
LOCUS       DBNGSG010000247         4502 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1097542, whole genome shotgun sequence.
ACCESSION   DBNGSG010000247 DBNGSG010000000
VERSION     DBNGSG010000247.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4502)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4502)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4502
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1097542"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..1482
                     /locus_tag="AC158Q_06505"
     CDS             73..1482
                     /locus_tag="AC158Q_06505"
                     /inference="COORDINATES: protein motif:HMM:NF006056.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3339461.1"
                     /translation="MALQIIKNATIISVDPKVGVIHGGDILIDGGRIAEVAPRISADT
                     AQVLDGTDMIVLPGLVNAHIHTWEFPLRGIGANWVSKRDYHGNMHRNMAMYYRAGDVH
                     VANLLGALNQINHGTTTIFDWCHIVRDSEMTDAAIDGLEESGIRAVFARGTVKPPVRE
                     GQTPYYDIPFPREEIHRLRTGRFASDDRLVTLAMATLGPDYATYEVCAQDIKLARDYG
                     LLNSAHTWARKGQRKLEDGMWRLAKDGLLGPDHNISHGNCLEDDELKMVLDHGCTVSA
                     TCFTEMLNSERVAMLGRLLKYGAMPSLGTDCDPYFNSSMLWVMRHAFQHQREIDNRTL
                     HAQGRWPAVDSQHATHTRDALEWATMGGAKMMRMDSRIGSITPGKQADLIMINARRMN
                     IFPVLHGGDPVHAAVMYAESSDIDTVMIAGKLAKRGGKLLFPEKRLAALQEQLLASRQ
                     RIMREGNYTYQPAPNGPRP"
     gene            1508..2494
                     /locus_tag="AC158Q_06510"
     CDS             1508..2494
                     /locus_tag="AC158Q_06510"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3339462.1"
                     /translation="MARLTLTALLLAGTLAAPVLAAQAGPAATAWPAKPIRMIHGFIP
                     GGAVDITARLVAAGMTDAFGQQVIVDGRPGAGGTVGAAIVARGEPDGYTLFLMASGHA
                     ASAGLYRKLPYDAVRDFTMISMVAGNPFVLAATMNFPAKSVNDLVRMARAEPGKINYG
                     TGGVGTGMHLASLLFQNRAGIKMNHVPYKGGNAAPMALLSGEIPLIFNTPSGVETHVR
                     SKRMRVLAITTRNRFALWPDAPTIAESAVPGFDVKGWYAVAAPKNLPAGRVKLLNQTV
                     RNALKRPDIGGKLTELGAEVTPTSPDEAQRFLASEVARWGKLIRDEKIPPQN"
     gene            2697..3971
                     /locus_tag="AC158Q_06515"
     CDS             2697..3971
                     /locus_tag="AC158Q_06515"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF044949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HIR3339463.1"
                     /translation="MTELAIQLSLMAFEAAFAAAVLLALFRARRVLGLAALYTTVGVF
                     YYLATLLAATTFIKVAPGLLMSPGSVALFPACLFAVLLVYIREDANEARSMIYGLLAA
                     NVSASLLGIFVSQHLQSPLAVNPIALPPELFVQSPRLFIVGTLALFADTILIILAYEA
                     VSRFVRPLFLRIWVSLALVLVFDTLLFVTGGFIEHPAYREILLSGILGKVGAALVYAS
                     ALTLYLPRAAAVDPQTTDRIRPGLGDLFQVLTYRQKYEALRAQVTRDPLTGVHNRGFL
                     DDTLNAQLAAVRRGGAPLTVMMVDVDDFKRVNDAHGHAEGDRALRVIARALVRVARAS
                     DIVCRYGGEEFSLILPNTRLEAAAQLAGRIREEVPAACQREGIGGGMRITVTIGLAVC
                     PDDGTEPDALMRAADRRLYQGKDSGRDRVVAG"
     gene            4078..>4502
                     /locus_tag="AC158Q_06520"
     CDS             4078..>4502
                     /locus_tag="AC158Q_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339464.1"
                     /translation="MTTFGYLLPRAAVLGMLLVLAVPAPAVQDAPKSRDHPLLTRYPS
                     SHISEYEKNYNAVEFAVGGKGGAPQRKPIEGEATFIRYFHNNADKQPSPLQLIRNYQN
                     AIKAIGGEVVYERLPRDGDGGETTLKVTTGGKEVWVRVEP"
BASE COUNT          820 a         1446 c         1469 g          767 t
ORIGIN      
        1 cggtggtaat ctttcgcagc ggactcgagc ggtttcgtat cggtccagca tttggaattc
       61 aggagcgcga ttatggcact gcagatcatc aagaacgcca ccatcatctc ggtggatccc
      121 aaagtcggcg tcattcacgg cggcgacatc ctgatcgacg gcgggcgtat cgccgaggtg
      181 gctccccgca tcagcgcgga cacggcccag gtgctcgacg ggaccgacat gatcgtgctg
      241 ccgggactgg tcaacgccca tatccacacc tgggagttcc cgctgcgcgg catcggcgcg
      301 aactgggtga gcaagcgcga ttaccacggc aacatgcacc gcaacatggc catgtactac
      361 cgggccgggg atgtgcacgt ggccaatctt ctcggcgcgc tcaatcagat caatcacggc
      421 acaacgacca ttttcgattg gtgccacatc gtgcgcgatt ccgagatgac cgatgcggcg
      481 atcgacggcc tcgaggaatc cgggatacgc gccgtattcg cgcgcggcac ggtgaagcca
      541 ccggtgaggg aagggcaaac gccgtactac gatatcccgt ttccccgcga ggaaatccat
      601 cgcctgcgca ccgggcgctt cgcgtccgac gaccggctgg tcaccctggc catggcgacg
      661 ctgggaccgg actacgcgac ctacgaggta tgcgcgcagg acatcaagct cgcgcgcgac
      721 tacgggctgc tgaacagcgc gcacacctgg gcgcgcaagg gccagcgcaa gctcgaagac
      781 ggcatgtggc gcctggcgaa ggacggactg ctcggtcccg accacaatat ttctcacggc
      841 aactgcctgg aggacgacga gttgaagatg gtgctcgacc acggctgcac ggtttcggcc
      901 acgtgcttca cggagatgct caatagcgag cgggtggcaa tgctcgggcg cctgctcaag
      961 tacggcgcca tgccttcgct cggcaccgac tgcgacccgt acttcaacag ctcgatgctg
     1021 tgggtcatgc gccacgcctt ccagcaccag cgcgagatcg acaaccgcac gctgcacgcg
     1081 caaggccgct ggccggctgt cgacagccag cacgccacgc atacgcgcga tgcgctcgag
     1141 tgggcgacca tgggcggcgc caaaatgatg cgcatggaca gcaggatcgg ctcgatcacg
     1201 cccggcaagc aggccgacct catcatgatc aacgcgcgcc ggatgaacat ctttcccgtg
     1261 ctccacggtg gggatccggt gcacgcggcg gtgatgtacg cggaatcctc cgatatcgat
     1321 acagtgatga tcgcgggcaa gctcgccaag cgcggcggca agctcctgtt cccggagaag
     1381 cggcttgccg cgttgcagga gcagctgctg gcctcgcgcc agcgcatcat gcgggaaggt
     1441 aattacactt atcaaccagc gcccaacggg ccccgcccgt aggcccatgg gccgaggaga
     1501 accgcaaatg gcaagactga cattgaccgc gctgttattg gccgggaccc tggcggcgcc
     1561 tgtcctggca gcgcaggccg gccccgcggc aaccgcctgg cctgccaagc cgatacggat
     1621 gatccacgga ttcataccgg gcggcgccgt cgacatcacg gcccggctgg tggccgccgg
     1681 aatgacggat gctttcggac agcaggtcat cgtggatgga agaccggggg cgggcggcac
     1741 ggtaggcgcg gcgattgtcg cgcggggcga gcccgacggt tacacgctgt ttctgatggc
     1801 ctcgggccac gccgcttcgg ccgggcttta ccgcaagctg ccgtacgacg cagtcaggga
     1861 cttcacgatg atctcgatgg tcgcgggaaa ccccttcgtt cttgccgcaa cgatgaactt
     1921 tcccgccaag tcggtgaacg acctggtgcg catggccagg gccgagccgg gcaagatcaa
     1981 ctatggcacc ggcggcgtgg gaacgggcat gcacctcgcc tcgcttctgt tccagaaccg
     2041 tgccggcatc aagatgaatc acgtgccgta caagggcggc aacgccgcgc cgatggccct
     2101 gctgtccggg gagattccgc ttatcttcaa caccccgtcg ggagtggaga cccacgtcag
     2161 gagcaagcgg atgcgcgtgc tcgccatcac cacccgcaac cgctttgcac tgtggccgga
     2221 cgcgccgacc atcgcggagt cggccgtgcc cggattcgac gtcaagggct ggtatgcggt
     2281 ggcagccccg aaaaacctgc ctgcgggcag ggtgaagctg ttaaaccaga ccgtgcgcaa
     2341 tgcgctcaag cgccccgaca tcggcggcaa actgacggag ctcggcgccg aggttacgcc
     2401 cacttccccg gacgaagcgc agcgcttcct cgcgtcggaa gtggcgcgct ggggcaagct
     2461 gatccgggat gaaaagattc ccccgcaaaa ctagcgttaa atccggatgg tccgcaccgg
     2521 tttgacccgg cgtgtgcgaa cggaaggccc tgctgccgtt caacgcgcgg ttgccgggaa
     2581 accgggtgca ggatcatgtt ttttttgcgc ggaagcggat tgctgaaacg actattcgta
     2641 aactgtttcc ggccttttcc tccaatcctg caaatttgta acctgccgcg cgccggatga
     2701 ccgaactggc catacaacta tcgctgatgg ccttcgaggc cgcgttcgct gccgcggtgc
     2761 tgctcgcgct gttccgcgcc cgcagggtcc tcggtctcgc cgcgctctac accacggtcg
     2821 gggtgttcta ctatctggcg acgcttcttg cggcgaccac cttcatcaag gtggcccccg
     2881 ggctgctgat gagtccgggc tcggttgcgc tgtttccggc gtgcctgttt gccgtgcttc
     2941 tcgtctacat ccgcgaggac gcgaacgagg cgcgcagcat gatctacggc ctgctcgcgg
     3001 caaacgtgtc cgcaagcctg ctcggcattt tcgtcagcca gcacctgcag agcccgctcg
     3061 cggtgaatcc gatcgccttg ccgccggagt tgttcgtgca gtctccgcgc ctgtttatcg
     3121 tcggcacgct cgcgctgttc gccgacacca tcctcatcat cctggcctac gaggcggttt
     3181 cgcgtttcgt gaggccgctg tttctgagga tctgggtctc gcttgcgctg gtgctggtat
     3241 tcgatacgct gctcttcgtc accggcggct tcattgagca cccggcttac cgggagatcc
     3301 tgctctcggg aatcctcggc aaggtcggcg cggcgctggt ctacgcgtcg gcgctgaccc
     3361 tgtatctgcc gcgggcagca gccgtcgacc cacagaccac ggacaggatc cggcccgggc
     3421 tcggcgacct gtttcaagtg ctcacctacc ggcaaaaata cgaggcgttg cgcgcgcagg
     3481 tcacgcgtga ccccctgacg ggagtgcaca atcgcggctt tctcgacgac acactgaatg
     3541 cgcagctcgc ggcggtgcgg cgcggcgggg cgccgctcac cgtgatgatg gtggacgtgg
     3601 acgatttcaa gcgggtcaac gatgcccacg ggcacgccga gggagaccgt gcgctgcggg
     3661 taatcgcgcg ggcactggtg cgcgtagcgc gcgcttcgga catcgtctgc cggtacgggg
     3721 gcgaggaatt ctccctcatt ctccccaaca cgcgcctcga ggccgcggcg caactggccg
     3781 gccgcatacg tgaggaggtg cccgcagcct gccagcgcga gggtataggc ggcggtatgc
     3841 ggatcaccgt taccatcggc ctcgcggtct gcccggacga cggcacggag cccgacgcgc
     3901 tcatgcgcgc ggcagaccgg agactctacc agggcaagga cagcggtcgc gaccgggtgg
     3961 tggcagggtg agcggcagga ggcggggcag actggtcaat aagaggaaca agttcctatc
     4021 ggatgaatcg cggccagtgc aattcttgaa accagggtcg acaatagggg ggatgacatg
     4081 actacgtttg gatacctgct gccacgggcc gcggtgctcg gaatgctgtt ggtccttgcc
     4141 gtgccggctc cggctgtcca ggatgcgccc aaatccagag accatccgct gctgacgcgc
     4201 tacccgtcct cgcacatcag cgagtacgag aaaaactaca acgcggtgga gttcgcggtc
     4261 ggaggcaaag gcggcgcgcc ccagcgcaag ccgatcgagg gcgaggccac attcattcgt
     4321 tactttcaca acaacgcgga caagcagccg agcccgctgc agctcatccg caattaccag
     4381 aacgcgatca aggcgatcgg cggcgaggtg gtgtacgagc gcctgccccg cgacggcgac
     4441 ggcggggaga ccacgctgaa ggtgacaacc ggcggcaagg aagtgtgggt gcgcgtcgag
     4501 cc
//
LOCUS       DBNGSG010000248         2685 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_418157, whole genome shotgun sequence.
ACCESSION   DBNGSG010000248 DBNGSG010000000
VERSION     DBNGSG010000248.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_418157"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="AC158Q_06525"
     CDS             <1..850
                     /locus_tag="AC158Q_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR3339465.1"
                     /translation="EDALIERIRNEGDKSPADVLITVDAGRLWRAEQLGLFQPVKSKV
                     LETRIPSSYRHPQGLWFGFSARARIIAYNKERVKPGDISSYEDLASPRWKGRICTRSA
                     GHVYNLSLISSLVNYLGEAKTEEWARAVASNLARSPKGGDTDQLKAVAAGECDLGISN
                     TYYYVRLLKSKRPDEKAAAGKIGVILPNQKDRGTHVNISGGGVLKHAPNREAAVRFLE
                     YLASDEAQSYFANGNNEWPVVPSVKLDNAALATLGTFKTDSLNLGVIGRNQPTAQKIA
                     DRSGFK"
     gene            complement(878..2548)
                     /locus_tag="AC158Q_06530"
     CDS             complement(878..2548)
                     /locus_tag="AC158Q_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3339466.1"
                     /translation="MNSFIGAAVFRNRGWNAVSVAAGLAACVLAVPVLVVLGSLFLPA
                     GDAWGHLVATVLPEYIRNTLWLMLGVAIGVTVFGVATAWLTTLCRFPGRGFFEWALIL
                     PLAVPAYVMAYAYTDFLQFTGPVQTWLRGYFAWGPREYWFPEVRSVGGAAVIFTFAFY
                     PYVYLLARAAFLEQAAGMIEAGRSLGYGPWSSFFRLALPLARPGIAAGVTLALMETLA
                     DFGTVTYFALPTFTTGIYRAWLSLGDRTAAAQLAAALLGFVVLVLLVERVSRGRARFD
                     ERSVRRRLVPRQLRGLPAIVALAVCLAPLMIGFVLPAAILLDLSLSGGDFHFGARYFR
                     LVANSVSVSALTAVCAVALALLMAYAARSGGNAIALAAHRLASLGYAVPGAVIAVGIL
                     IPVTRLDHALSGWLLSWFGISTGLILTGGIAALVYAYLVRFIAVALQSVEAGLTKVTP
                     TMDDAARSLGLTPMATLLRVHTPLLLPSVLTAALMVFVDVMKELPATFMMRPFNFDTL
                     AVHAYNLASDERLSELAVPALTIVAVGVVPLVLLSRSLVRARVAAEQG"
BASE COUNT          595 a          879 c          857 g          354 t
ORIGIN      
        1 cgaggacgcg ctgatcgagc gcatccgcaa cgaaggcgac aagagcccgg ccgacgtgtt
       61 gatcaccgtg gatgcgggcc ggctgtggcg tgccgagcag ttagggctgt tccagccggt
      121 caagtcgaag gtgctggaaa cacgcatccc ctcctcctac cggcatccac aaggcctgtg
      181 gttcgggttc tccgcgcggg cgcggatcat tgcctacaac aaggagcggg tgaagccggg
      241 cgatatttcg agctacgagg acctcgcgag tccgagatgg aaaggcagga tctgcacgcg
      301 ctcagcgggc catgtctaca acctctcgct catcagctcg ctggtgaatt acctgggcga
      361 ggcgaagacc gaggaatggg ccagggccgt cgcctccaac cttgcccgct cgcccaaggg
      421 tggtgacacc gatcagctca aggccgtggc cgccggggaa tgcgatctcg gcatcagcaa
      481 cacctactac tatgtccgcc tgctcaaatc gaagaggccc gacgagaaag cggcagccgg
      541 caaaatcggg gtgatcctgc ccaaccagaa ggatcgcggc acgcacgtca atatttccgg
      601 cggcggggta ttgaagcacg cgcccaaccg ggaagccgcc gtccgcttcc tggagtacct
      661 ggccagcgat gaggcgcaga gttattttgc caacggcaac aacgagtggc cggtcgtccc
      721 gtcagtgaaa ctcgataacg cggcactggc gacactggga acattcaaga ccgactcgct
      781 gaacctgggc gtcatcggca gaaaccagcc gaccgcgcaa aaaatcgcgg accgctcggg
      841 attcaaataa gggcggaggc gcctcgagcc gcgctcacta cccctgttcc gcggcgaccc
      901 gcgcgcgaac cagggagcgg gacagcaaaa ccagcggcac gacacccacc gcaacgatgg
      961 tgagcgcagg cacggcgagc tcgctcaggc gctcgtcgct cgccaggttg taggcatgca
     1021 cggccagcgt gtcgaaattg aacggccgca tcatgaaagt tgccggcagc tccttcatga
     1081 cgtccacgaa caccatcagg gcggcggtca gcacgctggg caacaggagg ggcgtgtgca
     1141 cccgcaggag agtggccatg ggcgtcaacc ccaggctgcg cgcggcgtca tccatggtgg
     1201 gggtgacctt ggtcaacccc gcctccacgc tctgcagcgc cacggcgata aagcgcacca
     1261 ggtacgcgta taccaaggca gcgatgccgc cggtgagtat cagccccgtc gagatcccga
     1321 accaggacag cagccagccg gacaaggcgt gatcgagccg cgtcacgggt atcaggatgc
     1381 ccaccgctat caccgcgccc ggcacggcat accccagcga agcgagacgg tgggcggcca
     1441 gcgcgatggc gttgccgccg ctgcgcgcgg cgtacgccat gagcaatgcc agtgccacgg
     1501 cacataccgc agtgagcgcg gaaaccgata cgctgttggc aaccagcctg aaataccgcg
     1561 ctccgaaatg aaagtccccg cccgatagcg aaagatcgag caggatggcg gccggcagaa
     1621 cgaacccgat catgagcggg gccaggcaga ctgccagcgc gacaatcgca ggcaggccgc
     1681 gcaattgccg tggaaccagg cggcggcgca ccgagcgctc gtcgaagcgt gcccggccgc
     1741 ggctcacccg ctcgacgagc agcaccagca cgacgaagcc cagcagcgca gcggcgagct
     1801 gcgccgcggc cgtgcggtcg cccagcgaaa gccaggcgcg gtatatcccg gtggtgaagg
     1861 tcggcagcgc gaaataggtg acggtgccga agtcggccag cgtttccatg agagcgagcg
     1921 tcacgcccgc cgcgatgccc gggcgcgcca gcggcaaagc gagccggaaa aaactgctcc
     1981 atggaccgta accgaggctg cggccagcct cgatcattcc cgctgcctgc tcgagaaaag
     2041 cagcgcgagc cagcagatac acgtagggat agaaagcaaa ggtgaagatc accgcggcgc
     2101 cgccgaccga gcgcacctcc gggaaccagt actccctggg accccaggcg aaatagccgc
     2161 gcagccaggt ctgcaccggc cccgtgaact ggagaaaatc ggtgtaggcg tacgccatca
     2221 cgtacgccgg taccgcaagc ggaagtatca gcgcccattc gaaaaaaccg cgccccggga
     2281 agcggcacag ggtcgtcagc caggccgtgg caacgccgaa caccgtcacg ccgatcgcaa
     2341 cgcccagcat gagccacagc gtgttccgga tatattccgg caggacagtc gcaaccaggt
     2401 gcccccacgc atccccggca ggcaagaata agctgcccag cacgaccagc accggcaccg
     2461 ccagcacgca ggccgccagg ccggccgcga ccgatacggc gttccagccc cggttccgga
     2521 agacggccgc gccgatgaat gaattcatgg ctcgctgcac ggcgtgtcga cccgagtgta
     2581 gatagttacc gatcgcatta cccggttagt ccttccgcgg taatcgtatg aatatcaagg
     2641 aggtttattc tatcaatcac agggcgacat atccaggaaa atgag
//
LOCUS       DBNGSG010000249         1473 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1946431, whole genome shotgun sequence.
ACCESSION   DBNGSG010000249 DBNGSG010000000
VERSION     DBNGSG010000249.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1946431"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="AC158Q_06535"
     CDS             complement(<1..246)
                     /locus_tag="AC158Q_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HIR3339467.1"
                     /translation="MSRRRVVVTGLGIISPVGNGAPEAWKNILAGASGISRITRFDAS
                     AFTSQIAGEVKGFDPAQVLSVKEARRFDTFIHYGLAAS"
     gene            complement(271..510)
                     /gene="acpP"
                     /locus_tag="AC158Q_06540"
     CDS             complement(271..510)
                     /gene="acpP"
                     /locus_tag="AC158Q_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR3339468.1"
                     /translation="MENIEQRVKKIVAEQLGVNEADVKSESSFVNDLGADSLDTVELV
                     MALEEEFETEIPDEEAEKIATVQQAIDYIKSHAKA"
     gene            complement(656..1399)
                     /gene="fabG"
                     /locus_tag="AC158Q_06545"
     CDS             complement(656..1399)
                     /gene="fabG"
                     /locus_tag="AC158Q_06545"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HIR3339469.1"
                     /translation="MNLESKIALVTGASRGIGQAIALELARCGAVVVGTATSASGAAG
                     IGQKLTSAGAKGAGLALNVNHPQQTEAAVQDIQKRFGDIAILVNNAGITRDNLLVRMK
                     DEEWDEVMATDLKSVYRLSRLVLRSMMKARYGRIINITSVVGMTGNAGQTNYAAAKAG
                     IIGFSKALAREVGSRNITVNCIAPGFIDTDMTRALSEQQRTALIETIPLGRLGRPEDV
                     AAAVAFLASPAADYVTGCTLHVNGGMFMD"
BASE COUNT          248 a          459 c          478 g          288 t
ORIGIN      
        1 cgacgcggca agcccgtaat ggatgaaggt atcgaagcgc cgggcttcct ttacggaaag
       61 gacttgcgcc ggatcgaagc ctttcacctc gcccgcgatc tgggaggtga aagcggaagc
      121 gtcgaagcgg gtgatgcggg agatgcccga cgcgccggcg aggatgttct tccaggcttc
      181 aggggccccg ttgccgaccg gggagatgat gccaagcccg gtgacgacga ctctgcgcct
      241 ggacaaattc gctccggacg ggggtggaac tcaggccttg gcgtggctct tgatgtagtc
      301 gatggcctgc tggacggtgg cgatcttctc ggcttcctcg tccgggatct cggtctcgaa
      361 ttcttcctcg agcgccatca cgagctccac ggtgtcgagc gagtccgcgc cgagatcgtt
      421 gacgaacgaa gactcgctct tgacgtctgc ctcgttgaca cccagctgct cggcaacaat
      481 tttcttgact cgttgctcga tgttctccat tcagttcccc tttcctcgac aggagtgaaa
      541 agaccgcagg taaccccggc gcgcggcacg gtgcgcggtg gccggattta agcagggcgc
      601 tattttacca gattcatccg tggacaaaat tcccgttttt caaggatctg ggccatcaat
      661 ccatgaacat gccgccgttg acgtgcaatg tgcatcccgt cacgtaatcg gccgccggag
      721 aggccagaaa tgcgacggcg gcagccacgt cctccggccg ccccagccgc cccagcggga
      781 tggtctcgat cagggcagtg cgctgctgct ccgagagcgc gcgcgtcatg tcggtgtcga
      841 tgaatccggg ggcgatgcag ttgacagtga tgttacggct gccgacctcg cgcgcaagcg
      901 ccttggaaaa cccgatgatg ccggccttcg cggcggcgta gttggtctgc ccggcattgc
      961 cggtcatacc cacaaccgaa gtgatgttga tgatacggcc gtagcgcgcc ttcatcatcg
     1021 agcgcagcac caggcgggac aggcggtaga cggatttcag gtctgtcgcc atgacctcat
     1081 cccattcctc gtccttcatg cgcaccagca ggttgtcgcg ggtgatgccg gcgttgttga
     1141 cgaggatggc gatgtcgccg aagcgcttct ggatatcctg cacggccgct tccgtctgct
     1201 gcgggtgatt gacgttgagc gccagcccgg cgcccttggc gcccgccgag gtgagcttct
     1261 ggccaatgcc cgccgcacct gacgcgctgg tcgcggttcc gaccaccacc gcgccgcagc
     1321 gcgcgagctc gagcgcaatc gcctgaccga tgccgcgcga ggcaccggtg acgagggcaa
     1381 tcttgctttc gaggttcatg gcttgagcgc cagcagcgcc tgctggagcg actgcgcgtc
     1441 cgcgagggca tggctctgga gggcgccgtc gct
//
LOCUS       DBNGSG010000250         3386 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2286683, whole genome shotgun sequence.
ACCESSION   DBNGSG010000250 DBNGSG010000000
VERSION     DBNGSG010000250.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2286683"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="AC158Q_06550"
     CDS             <1..215
                     /locus_tag="AC158Q_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HIR3339470.1"
                     /translation="TIKMLALSFLILVGVALIGEGWGLHIPKGYIYFAMAFSVAVEML
                     NIRMRAKRMNAVQLRKQVPGARPAGQ"
     gene            complement(225..1088)
                     /locus_tag="AC158Q_06555"
     CDS             complement(225..1088)
                     /locus_tag="AC158Q_06555"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA dioxygenase family protein"
                     /protein_id="HIR3339471.1"
                     /translation="MLSVHRSSAPLGAEIRGVDLSRDVSDEIFRRIVGLFHEHEVIFF
                     RDQDLAPEQHVAFSRRFGALEHHVRQECCRPGYPELFVVSNILENGRPIGSQDAGLFW
                     HSDLCYLKEPSRGSLFYAREVPTDAQGIPRGDTMFASATAAYDALPDADKRTLDGLKA
                     VNSYAKGYYRDRKSGPRKELTEEQKRRTPDVEHPIVRTHPYTGKKCLFLNEGYTSRVV
                     GMSEPESNALLARLFEHCTRPEFVYRHQWRVGDFLMWDNCSTQHRAVIDYALPQRRRM
                     ERATLVGNAPF"
     gene            complement(1129..2322)
                     /locus_tag="AC158Q_06560"
     CDS             complement(1129..2322)
                     /locus_tag="AC158Q_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HIR3339472.1"
                     /translation="MTAPMPTTKPAFHWEDPLLLEDQLTDDERMVRDAARAYCQEKLM
                     PRVLEANRHEKFDREVMNEMGSLGFLGSTLSGYGCAGVNHVCYGLVAREVERVDSGYR
                     STLSVQSSLVMYPIHEFGTETQRRKFLPRLASGEWIGCFGLTEPDVGSDPGGMVTRAR
                     KVDGGYVLQGAKMWISNAPYADVFVIWAKDDTGEIRGYLLEKGMKGLTTSKIEGKFSL
                     RVSPTGEVSMDQVFVPEENLLSGVSGLKGPFSCLNKARFGIAWGALGAAEFCWHAARN
                     YSLERKQFGRPLAANQLVQLKLANMQTEIALGLQAALRLGRLMDEERATPEMISLCKR
                     NACGKSLDIARVARDMHGGNGIADEFHVIRHVMNLEAVNTYEGTHDIHALILGRAQTG
                     IPAFS"
     gene            complement(2392..2934)
                     /locus_tag="AC158Q_06565"
     CDS             complement(2392..2934)
                     /locus_tag="AC158Q_06565"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR3339473.1"
                     /translation="MKFCSSCGAPVALEVPPGDTLPRHVCGACHTIHYQNPRMVVGCI
                     PEWEDRILLCRRAIEPRHGLWTVPAGYMENGETTFQGALRETLEEANARVEIGSLYAL
                     YNIPHINQVYILFRARLLDRNVSPGAETLEVRLFSESEIPWDRIAFASVRNTLSHYYA
                     DRRSGEYRFHMGTIEQPRKV"
     gene            3025..>3386
                     /locus_tag="AC158Q_06570"
     CDS             3025..>3386
                     /locus_tag="AC158Q_06570"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3339474.1"
                     /translation="MTRKQSLGIFAAILFCLAAIGRLEAALDAGEHEFIITHEGLRRS
                     YLVQVPPVAGAGKPLPVVVNFHGGGSNAKVQKAYSRMDAAASRDGYIAVYPNGTGGIG
                     GRFLTWNAGPCCGPAAANR"
BASE COUNT          574 a         1052 c         1099 g          661 t
ORIGIN      
        1 ccacgatcaa gatgctggcg ctgtcattcc tgatcctggt cggcgtcgcg ctgatcgggg
       61 agggctgggg cctgcatatc ccgaagggct acatctactt cgcaatggcg ttttcggtcg
      121 cggtcgagat gctgaacatc cgcatgcgcg cgaagcggat gaacgcggtc cagctgcgca
      181 agcaggttcc gggcgcgcgg cccgcaggac aatagggggg tgcctcagaa gggcgcgttg
      241 cccaccaggg tggcgcgctc catgcgccgg cgctgcggca gcgcgtagtc gatcaccgcg
      301 cggtgctgcg tggagcagtt gtcccacatc aggaagtccc cgacccgcca ctgatggcgg
      361 tagacgaatt cgggacgcgt gcagtgctcg aagaggcggg cgagcagcgc gttgctttcc
      421 ggctcgctca tgccgacgac gcgcgaagtg tagccttcat tcaggaacag gcacttctta
      481 ccggtgtagg ggtgcgtgcg cacgatcgga tgctcgacat cgggcgtgcg ccgcttctgc
      541 tcttcggtga gttccttgcg cggtccgctc ttgcgatcgc ggtagtagcc cttggcgtag
      601 gaattgaccg ccttcaggcc atccagcgtg cgcttgtccg catcgggcag cgcgtcgtag
      661 gcggcggtcg cgctggcgaa catcgtatcg ccccttggaa tgccctgcgc atccgtcggg
      721 acttcccgcg cgtagaacag cgagccgcgg ctcggttcct tcaggtagca gaggtccgag
      781 tgccagaaca gcccggcgtc ctgggagccg atgggcctgc cattttcaag gatattggaa
      841 accacgaaga gttccgggta gcccggccgg cagcattcct gccgcacgtg gtgctcgagc
      901 gcgccaaagc ggcggctgaa ggcgacatgc tgttccggcg ccagatcctg gtcgcggaag
      961 aatatgacct cgtgctcgtg gaacaggccc acgatgcggc ggaaaatttc gtcactgacg
     1021 tcgcgggaca gatcgacgcc gcgaatttcg gcgccgagtg gcgcgctgga tcggtgtacg
     1081 ctcaacatga tctttgaaaa ttctctccgc gccgaatatt atcagagctc agctgaatgc
     1141 ggggatgccg gtctgggcgc gtccgaggat gagggcgtgg atgtcgtggg tgccctcgta
     1201 ggtgtttacc gcttccaggt tcatgacgtg gcggatgacg tggaactcgt cggcaatgcc
     1261 gttgccaccg tgcatgtcgc gggcgacgcg cgcgatgtcg agcgattttc cgcacgcgtt
     1321 gcgcttgcac agggagatca tctcgggcgt ggcgcgttcc tcgtccatca gccgcccgag
     1381 ccgcagtgcg gcctgcagcc ccagcgcgat ctcggtctgc atgttggcga gcttcagctg
     1441 cacgagctgg ttggccgcga gggggcggcc gaactgcttg cgctccaggg agtaattgcg
     1501 cgccgcgtgc cagcagaact cggcggcacc cagtgcgccc caggcgatgc cgaagcgagc
     1561 cttgttgagg caggagaagg gtcccttcag tcccgatacg ccggacagca ggttttcttc
     1621 cggaacgaac acctggtcca tggaaacctc gccggtgggc gacacgcgca ggctgaactt
     1681 gccttcgatt ttcgaggtgg tgagcccctt catgcctttt tccagcaggt aacctcggat
     1741 ctcgccggta tcgtccttgg cccagatcac gaacacgtcg gcatagggcg cgttgctgat
     1801 ccacatcttg gcgccctgca gcacgtagcc gccgtcgact ttcctggccc gcgtcaccat
     1861 gccgccggga tccgagccga catcgggctc cgtcagcccg aagcagccga tccattcgcc
     1921 gctggcgagc ctgggaagga atttcctgcg ctgggtttcg gttccgaact cgtgaatggg
     1981 gtacatgacg aggctcgatt gcaccgacag cgtcgagcgg tagccgctgt ccacgcgctc
     2041 cacttcgcgc gccaccaggc cgtagcacac atgattgacg ccggcgcagc cgtaacccga
     2101 aagggtcgag ccgagaaatc cgaggctccc catttcgttc ataacctcgc ggtcgaattt
     2161 ctcgtgccgg ttcgcttcca gcacccgcgg catgagcttt tcctggcaat aggcgcgcgc
     2221 ggcgtcgcgc accatgcgct cgtcatcggt cagctgatct tccagcagca gtggatcttc
     2281 ccaatggaaa gcaggcttgg tcgtcggcat gggcgcggtc attcattttt ctccagggga
     2341 tgcgcgtatt ttacgcttcg acccgggttt ccggctggat ccgccgagcc gtcacacctt
     2401 tctcggctgc tcgatagtcc ccatgtgaaa gcgatattct ccgctccggc ggtccgcgta
     2461 gtaatggctc agtgtgttgc ggacgctggc gaaagcgatt ctgtcccagg gaatctcgct
     2521 ttccgaaaac aaccggacct cgagcgtttc ggcgccggga ctcacattcc tgtccagcag
     2581 gcgggcgcga aacagaatgt agacctggtt gatatgcggg atattgtaca gggcgtagag
     2641 cgagccgatc tcgacgcggg cattggcttc ctccagcgtc tcgcgcaacg cgccctggaa
     2701 agtggtttcg ccgttctcca tgtaaccagc cggcacggtc cacaatccgt ggcgcggctc
     2761 gatggcgcgg cggcacagca ggatgcgatc ttcccactcg ggaatgcagc ccaccaccat
     2821 gcgcggattc tggtaatgaa tcgtatggca ggcgccgcat acgtggcggg gaagcgtgtc
     2881 gccaggcggc acttcgagcg cgaccggtgc accgcagctg ctgcagaatt tcatgacgat
     2941 gcgggctcca tcgggttgtg actatacggt aacattcctg cgtcgcgacc gcttgctttc
     3001 gcgcgcgctg gatagatcga atccatgact cgcaaacaat ccctgggcat tttcgccgcc
     3061 atcctttttt gcctggcggc gatcggcagg ctggaagccg cgctcgacgc gggggagcac
     3121 gaatttatca tcacgcacga gggcctgcgt cgcagctacc tggtgcaggt gccgccagtt
     3181 gccggcgcgg gcaagccgct acccgtcgtt gtcaactttc acggtggtgg cagcaatgcg
     3241 aaagtccaga aagcctactc acgcatggac gccgctgcca gccgggacgg ctatatcgcg
     3301 gtgtacccga atggcactgg cggcatcggc ggccgcttcc tcacctggaa cgcagggccc
     3361 tgctgcggcc ctgcggcggc gaaccg
//
LOCUS       DBNGSG010000251         1629 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3135797, whole genome shotgun sequence.
ACCESSION   DBNGSG010000251 DBNGSG010000000
VERSION     DBNGSG010000251.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3135797"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="AC158Q_06575"
     CDS             <1..366
                     /locus_tag="AC158Q_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339475.1"
                     /translation="NPCDPVDIAYAFSVFTHTSEEATGAILRAVRSVLPRGGLFVTSI
                     RPIEFWERINKKVPVAVKDMEHAHRTRGYAFTPKKEMEHFGSTSMKPEYLAGPDWNVL
                     GTERSLVDPVQLVVTLQAQ"
     gene            587..>1629
                     /locus_tag="AC158Q_06580"
     CDS             587..>1629
                     /locus_tag="AC158Q_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR3339476.1"
                     /translation="MPELSLDFPAGLSGWVLVETRLDSRYRNCFSSLRVHRGSPEGRT
                     EEVPLPVFMDGRIVELVELSRDTARLSWRPPDGGQFDASLLRIRRVGWAERTVRMAIR
                     VVRTYLRVSEDHRRESGLTPGRSLLDLPRAYRIATTFRVRYQLLNYPEWIERFDTLRE
                     PDVSGIRAHIARFAARPHFHVLLTSDAGGAPAARATLASLRAQLYANFSCNVLDPPGT
                     LGAGFDTAAELKGVGTQSIFVPQPDVAGWLSGFNAALARAGADEWVMLLRAGDTLPAH
                     ALYWFACEAQARPDAAVFYSDDDSLDEQGRRVSPRLKPDWSPAHLHSTHYVGSAAILR
                     AGSVAAAGGVDPD"
BASE COUNT          267 a          532 c          521 g          309 t
ORIGIN      
        1 aacccgtgcg atcccgtgga catcgcttac gctttttccg tgttcacgca cacatcggag
       61 gaagcgaccg gggccatact tcgcgcggtg cgctcggtgc tgcccagggg gggactgttc
      121 gtcaccagca ttcggccgat cgaattctgg gagcgcatca acaagaaagt gcccgttgcc
      181 gtgaaagaca tggaacacgc gcaccggacc cggggttacg ctttcactcc gaaaaaggag
      241 atggagcatt tcggcagcac ttcgatgaaa cccgaatacc tcgccggccc cgactggaac
      301 gtcctcggca cggagcgcag cctggtcgac ccggtccagc tggtcgtcac cctccaggcg
      361 caatagaatc aaatgcaata aaaggcgcca ggccgctttt ctccccgggt tcctgtcaac
      421 acccgtcaac accgtaaatg aacgggattc acgggattta caggatcaag cctgtttagg
      481 cggaaatggg tctttgcttg aatcctgtta atcttgttaa tcctgtctat tttggatctt
      541 gacgggtatt ccgattcgtt cgtgaatcct gtccgttacg attctcatgc ccgaactgag
      601 tctcgatttc cccgccggct tgtcgggatg ggtcctcgtc gagacgcgtc tggacagccg
      661 ctaccgcaat tgcttttcca gcctgcgagt gcatcgcggc tcgcccgaag gcaggacgga
      721 agaggttccc ctgcccgtct tcatggacgg ccgcatcgtc gagctggtcg agctgtcgcg
      781 cgacaccgcc cggctgagct ggcgcccgcc cgatggcggg cagttcgacg cctccctgct
      841 caggatccgg cgggtcggat gggccgagcg caccgtgcgc atggcgatcc gcgtggtgcg
      901 gacctacctg cgggtgtccg aggaccatcg ccgcgagtcg ggcctgacgc ccgggcgctc
      961 gctgctcgac ctgccgcgcg cctaccgcat cgccaccacg ttccgcgtgc gttatcagct
     1021 gctgaattac ccggaatgga tcgagcgatt cgacaccttg cgggaaccgg acgtctccgg
     1081 aatccgggcg cacatcgcgc ggttcgcggc gcgcccgcat ttccacgtgc tcttgacgag
     1141 cgatgcggga ggcgcgccgg ccgcgcgggc gacgctggcg tcattgcgcg cccagctcta
     1201 cgcgaatttc agctgcaacg tgctggatcc gcccggcacg ctcggagccg gcttcgatac
     1261 cgccgccgaa ttgaagggtg tgggcacgca atcgattttc gtgccgcagc ctgacgtggc
     1321 tggctggctg tccgggttca acgccgcgct cgccagggct ggcgcggatg agtgggtcat
     1381 gctgctgcgc gccggcgaca cgctgcccgc gcatgccttg tactggttcg cctgcgaagc
     1441 gcaggcgcgg ccggacgcgg cggtcttcta ttcggacgac gactccctgg acgaacaggg
     1501 acggcgcgtg agcccacgcc tcaagcccga ctggtcgccg gcgcacctgc attccacgca
     1561 ttacgtgggc tcggcggcga tcctgcgcgc cggctccgtg gcagcagcgg ggggcgtgga
     1621 tcccgatag
//
LOCUS       DBNGSG010000252         4656 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2455965, whole genome shotgun sequence.
ACCESSION   DBNGSG010000252 DBNGSG010000000
VERSION     DBNGSG010000252.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4656)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4656
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2455965"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..1500)
                     /locus_tag="AC158Q_06585"
     CDS             complement(130..1500)
                     /locus_tag="AC158Q_06585"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HIR3339477.1"
                     /translation="MNRVLIALVRHALTAALLAAPAAVAQPQADARGMPTLAPLVNEV
                     TPAVVNISVVTRAPIENNPLFRDPFFRRFFNLPDKPQRQEQAAGSGVIVDAARGLILT
                     NHHVIRDAEQAIVTLKDRRQFPAKLVGADPGTDIAVLQIEAQKLTAIRIGDSDTLQVG
                     DYVIAIGNPFGIGQTVTSGIVSALGRSGLSPEGYEDFIQTDASINPGNSGGALVNLRG
                     ELVGINSAILSASGGNLGIGFAVPSNMARIVMNQIVRFGEVRRGRLGIDMVDLTPAIA
                     KKLGVSAPEGVVITTVRTGSPAEKAGLRANDVVIALNGRPVRAAADLRARLGLTPVGE
                     EIELRVLRARDGLNIRVRIAAPQEQPDAKGQAVAQLPGMSVIEIERGSPLYQRLRGGG
                     LVVSAVEDDSRAHQAGFRAGDILYAVNRRRVQTIAEFQAALRGAQGGYAVSLLRGDFN
                     VTIIVR"
     gene            complement(1550..3547)
                     /locus_tag="AC158Q_06590"
     CDS             complement(1550..3547)
                     /locus_tag="AC158Q_06590"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004766 - spermidine synthase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HIR3339478.1"
                     /translation="MLLYALTIFTSAFLLFLVQPVMAKQILPWFGGSAAVWTTCLVFF
                     QFLLLAGYAYSDWTTRRLDPRRQAALHVALIAASLASLPIIPDAGWKPAGDEDPTWRI
                     LGLLTVTIGLPYFLLSTTGPLVQAWFARTFPTGTVYRLFALSNFASLLALIAYPFAVE
                     PWISTVHQSWSWSAAYVLFAALCAASAIYGARARPAAVATKAAAGPPPSLRDYGAWLL
                     LSAMGSFMLLAVTNHITHDVASVPFLWILPLTIYLVTFILCFEGGGWYRRKVFIVPLL
                     AIVSAMAWALHAETGLMDIKLAIPLALAGLFVMCMFFHGELAAIKPEPRYLTRFYLMV
                     SLGGALGGVAVGMVAVKLFSTYYEFGIGLVATLAIAAYVSRFMNFIVPMLVFAAIGFS
                     GFHVHQYITALTQGARVMTRNFYGTLRVKDVGAGDDAVRRLMHGVIMHGEQYQARERR
                     NEPTTYYGATSGIARVLKTLDASALRVGVVGLGTGTLAVYGRKGDVYRFYEINPQVVE
                     VAQREFSFLGDSGAGIETILGDARLAMEREAPQNYDVLAIDAFSSDSIPVHLITREAL
                     AVYLRHLKPGGVIAFHVTNRFLHLAPVVERIAGEAGLHTALIDDGAEDSELASTDWVL
                     VTRDKALLERPGIAQHVSPVERIPGLGVWTDDFNNLFQILK"
     gene            3648..4523
                     /locus_tag="AC158Q_06595"
     CDS             3648..4523
                     /locus_tag="AC158Q_06595"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008684 - 2-oxopent-4-enoate hydratase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HIR3339479.1"
                     /translation="MSLPRHKTLVSLFALTAALSWPGDAEAYCPSRERVNTILRFREA
                     REPVRGLRSDLSMQDAECGRLRLVERLEAGDNRIVGYKAGLTSRAAQERFGVSSPVRG
                     VLLEQMLLADGAEVPFDFGARPVFEPDLLVVVKDAAIHQAKTHLDVLRALSLVIPFIE
                     LPDLLVAEGEKLSAPLIVSLNVGARLGMVGKGIPVRATPEFAAALAAMRVVVTDQKNR
                     ELASGKGAAILDHPLNAVLWLVRDLEKSRVRLKPGDRLSLGSFTAPLTPRPGLTVTVR
                     YEGLPGNPAVSVRFK"
BASE COUNT          815 a         1507 c         1621 g          713 t
ORIGIN      
        1 ggcgatcagg gagggcattc ggtgcacgct tcagttccgt tcgacatgcg cgaccaatgc
       61 ccgcgcgatg agcggcggac catcgtccgc ccgatcgcgc ggaggtaaac cggcgcgccg
      121 cgtccggctt taccgaacga tgatcgtaac gttgaaatcc ccgcgcagca gcgagacggc
      181 gtagccgccc tgcgcgccgc gcagggcggc ctggaactcg gcgatcgtct gcacgcgccg
      241 gcggttgacc gcgtaaagga tgtcgccggc gcggaagccc gcctggtggg cgcggctgtc
      301 gtcttccacc gcggacacca ccaggccgcc gccccgcagc cgctggtaga gcgggctgcc
      361 gcgctcgatt tcgatcacgc tcatgcccgg cagttgcgcc accgcctggc ccttggcgtc
      421 cggctgctcc tggggcgcgg cgatgcgcac ccggatattc agcccgtcgc gcgcgcgcag
      481 cacccgcagc tcgatttcct ctcccaccgg cgtcaacccg agacgcgcgc gcaggtcggc
      541 ggccgcgcgc accgggcgcc cgttcaacgc gatcaccacg tcgttcgcgc gcaaccccgc
      601 tttctccgcc ggcgagccgg tccgcaccgt cgtgatgacc acgccttccg gcgcgctcac
      661 gccgagcttc ttcgcgatgg cgggcgtgag atcgaccatg tcgatgccga gccgcccgcg
      721 ccgcacctcg ccgaagcgca cgatctggtt catcacgatg cgcgccatgt tggagggcac
      781 cgcgaaaccg atgccgaggt tgccgcccga cgcgctcagg atcgcgctgt tgatgccgac
      841 cagttcgccg cgcaggttga ccagcgcgcc gccggaattg ccggggttga tcgaggcgtc
      901 ggtctggatg aagtcctcgt aaccctcggg cgagagaccg ctcctgccga gcgcgctgac
      961 gatgccggag gtgacggtct ggccgatgcc gaacgggttg ccgatcgcga tcacgtaatc
     1021 gcccacctgc agcgtgtcgg agtcgccgat ccggatcgcg gtgagtttct gcgcttcgat
     1081 ctgcagcacc gcgatgtcgg tgccggggtc ggcgccgacg agcttcgcgg gaaactggcg
     1141 gcggtccttc agcgtgacga tggcctgctc ggcgtccctg atcacgtggt ggttggtgag
     1201 gatcagcccg cgcgcggcgt ccacgatcac ccccgagccg gcggcctgct cctgccgctg
     1261 cggcttgtcg ggcaggttga agaagcggcg gaagaacggg tcgcggaaga ggggattgtt
     1321 ctcgatcggg gcgcgcgtga cgaccgagat gttcaccacg gccggcgtta cttcattcac
     1381 cagcggcgcc agcgtcggca tcccgcgcgc atccgcctgc ggctgcgcca ccgcagcggg
     1441 cgcagccagc agcgcagcgg tcagagcgtg gcggacaagg gcgatcagca ctcgattcat
     1501 ggctgtaaac ctacaccact caccattcac cattcaccat tcacgcattt tacttaagaa
     1561 tctggaacag attattgaaa tcatccgtcc acacccccag cccggggatg cgctcgaccg
     1621 gtgacacgtg ctgggcgatg ccgggccgct ccagcagcgc cttgtcgcgc gtgaccagca
     1681 cccagtcggt gctggcgagc tcgctgtcct cggcgccgtc gtcgatcagc gcggtgtgga
     1741 gcccggcctc gccggcgatg cgctccacca ccggcgccag gtgcaggaaa cggttggtga
     1801 cgtggaacgc gatcacgccg ccgggtttca ggtgccggag gtagaccgcc agcgcctcgc
     1861 gcgtgatcag gtgcaccggg atggaatcgc tcgaaaacgc gtcgatcgcg aggacgtcgt
     1921 agttctgcgg cgcctcgcgc tccatcgcga gccgcgcgtc gcccagaatg gtctcgattc
     1981 ccgcgccgct gtcgccgagg aagctgaact ctcgctgcgc gacctccacg acctgcgggt
     2041 tgatctcgta gaagcggtag acatcgccct ttcttccgta gaccgcgagc gtgccggtgc
     2101 cgagcccgac gacgcccacc cgcagcgcgg acgcatcgag cgtcttcagg acgcgcgcga
     2161 tgccggacgt cgcgccgtaa tacgtggtcg gctcgttgcg cctctccctg gcctggtact
     2221 gctcgccgtg catgatcacg ccgtgcatca ggcgccgcac ggcgtcgtcg ccggcgccca
     2281 cgtctttcac gcgcagggtg ccgtagaaat tgcgcgtcat gacgcgggcg ccctgcgtca
     2341 gcgccgtgat gtactgatgg acgtgaaagc cgctgaagcc gatggccgcg aacaccagca
     2401 tgggcacgat gaaattcatg aaccgcgaga cgtacgccgc gatcgccagc gtcgcgacca
     2461 gcccgatgcc gaattcgtag taggtgctga acagcttcac ggccaccatg ccgaccgcga
     2521 ccccgccgag cgcgccgccc agcgacacca tcaggtagaa gcgggtcagg tagcgcggct
     2581 cgggcttgat cgcggcgagc tccccgtgga agaacatgca catcacgaac agcccggcca
     2641 gggcgagcgg tatcgccagc ttgatgtcca tcaggccggt ctcggcgtgc agcgcccacg
     2701 ccatggcgga cacgatcgcg aggagcggca cgatgaagac cttgcgccgg taccagccgc
     2761 cgccctcgaa gcagaggatg aaggtcacca ggtagatcgt gagcggcagg atccacagga
     2821 acggcaccga ggcgacgtcg tgcgtgatgt gattggtcac ggcgagcagc atgaaagagc
     2881 ccatcgccga gagcagcagc cacgcgccat agtcgcgcaa cgacggcggc ggcccggcag
     2941 cggccttggt cgcgacggcg gcgggacggg cgcgggcgcc gtagattgcg gaagcggcgc
     3001 acaaggcggc gaacagcacg taggccgcgc tccagctcca cgactggtgc acggtgctga
     3061 tccacggctc gacggcgaac gggtacgcga tcagcgccag cagcgaggcg aagttggaga
     3121 gcgcgaacag ccggtacacc gtccccgtgg ggaaggtgcg cgcgaaccac gcctgcacca
     3181 gcgggccggt ggtcgagagc aggaagtacg gcagcccgat ggtgacggtg agcagtccca
     3241 ggatgcgcca ggtcgggtcc tcgtctccgg ccggcttcca gcccgcgtcc gggatgatgg
     3301 gcagcgaggc gaggctcgcg gcgatcagcg caacgtgcaa tgccgcctgc ctgcgcggat
     3361 cgagcctgcg cgtggtccag tcggaatagg catagccggc gagcagcagg aactggaaga
     3421 acacgaggca ggtcgtccac accgcggcgg agccgccgaa ccacggcagg atctgcttcg
     3481 ccatgaccgg ctggaccagg aacagcagga acgcgctcgt gaagatcgtg agtgcgtaaa
     3541 gcagcattat tcttgttgga gaagggtggg gcgggtgggg atgggattcg ggaagcgcgt
     3601 attataccgg cggcaataat tcagtgatga gtgatgaatg atgagtgatg agtttgccca
     3661 ggcataaaac gcttgtaagc ctgtttgcgt tgacagcggc gctgtcgtgg ccgggcgacg
     3721 cggaggctta ttgcccgagc cgcgaacggg tcaacacgat actgcgcttc agggaggcga
     3781 gggaaccggt gcgcggtttg cgctcggatc tttccatgca ggatgcggag tgcggccgcc
     3841 tgcgcctggt cgagcggctg gaggcgggcg acaaccgcat cgtcggctac aaggcgggat
     3901 tgaccagccg cgcggcgcag gaacgcttcg gcgtctcgag cccggtgcgc ggcgtgttgc
     3961 tcgagcagat gctgctcgcg gacggcgcgg aggtgccttt cgatttcggc gcgcggccgg
     4021 tgttcgagcc ggacctgctg gtcgtggtca aggatgctgc cattcaccag gcgaagacgc
     4081 acctcgacgt gctgcgcgcg ctgtcgctgg tgatcccgtt catcgaattg cccgacctcc
     4141 tggtggcgga aggcgagaag ctctccgcgc cgctgatcgt gtcgctcaac gtgggcgcgc
     4201 gcctgggcat ggtggggaag ggcatcccgg tgcgggcgac gcccgaattc gcggcggcgc
     4261 tcgcggcgat gcgcgtggtc gtgaccgatc agaagaacag ggaactggcg agcggcaagg
     4321 gcgcggcgat cctcgatcac ccgctgaacg cggtgctgtg gctcgtgcgg gacctggaga
     4381 aatcccgcgt ccggctgaaa ccgggcgacc ggctgagcct ggggtcgttc accgcgccgc
     4441 tgacgccgcg gccggggctg acggtgacgg tgcgatacga gggattgccg gggaatccgg
     4501 cggtgtcggt gaggtttaag taaaacctga aattagccgc caagacgcca aggcgccaag
     4561 aaatgcaaaa ataaaattga accgccaaga ccgccaagaa agacaatcat ggaaccgctg
     4621 atgaacgccg atacacgccg ataaaatcaa gaacaa
//
LOCUS       DBNGSG010000253         2538 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3305705, whole genome shotgun sequence.
ACCESSION   DBNGSG010000253 DBNGSG010000000
VERSION     DBNGSG010000253.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3305705"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /locus_tag="AC158Q_06600"
     CDS             complement(<1..1202)
                     /locus_tag="AC158Q_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR3339480.1"
                     /translation="METLTHILNGFAVCLQPINLWYTFLGVFLGTIIGVLPGIGPSAG
                     IALLIPVTYGMNPTSALIMMAGIYYGTKYGGSTTAILINTPGEAASVMTAIDGYQMAK
                     KGRAGAALAISAVGSFIAGTLGVIGLTLFAIPLTSMALKFGPAEYFMLMFFAMTAVSS
                     LAGRSPAKAMISTVLGLMIATIGIDLQSGQPRYTMGVPELQDGVGFVIAVVGLFAIAE
                     VFSTMEELTKGTRPEIIRLKGKLWFTKNEWDRSLMPIMRGSVIGFVIGVLPGAGGTIA
                     SIMTYIWEKRLSKHPEEFGNGAIEGVAGPEAANNSDTAGAMVPLLTLGIPGGGATAVM
                     LGAFIMYGIQPGPLLFQNRPDLVWGLIDSMYVGNIMLLILNLPLIGLFVRLLYIPGGI
                     LMPLIMAIS"
     gene            complement(1210..1752)
                     /locus_tag="AC158Q_06605"
     CDS             complement(1210..1752)
                     /locus_tag="AC158Q_06605"
                     /inference="COORDINATES: protein motif:HMM:NF018979.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HIR3339481.1"
                     /translation="MMTDRVIFACTIVIAAVYLYATTLIPSLEIGDPLGPKAFPRLLG
                     IGLLIAAGLLFVEMWKDRKLQAPAPAAAAPRDVRHLWILAGVVVWTGVYYALLEKLGY
                     IVDSTIYLFALMAWFNRGKWVANGLTAVLYSVLSYIMFVKLDVNLPRGVEGISGISDA
                     VIVATNSVVNLFKSVPGFLK"
     gene            2038..>2538
                     /locus_tag="AC158Q_06610"
     CDS             2038..>2538
                     /locus_tag="AC158Q_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HIR3339482.1"
                     /translation="MSHNLFNSLQEFKYGGKAGKYYSLPALEKAGLGKISRLPVSFRV
                     VLESALRNCDGKKITEAHLRSMAAWKPKAKRSQEVPLVVARVLLQDMTGVPLVVDFAA
                     MREAAKAMGKDPEIIEPLCPGHLIMDHSVQIDNYGTPDALRKNMEIEFKRNRERYQFL
                     KWSAGAF"
BASE COUNT          517 a          817 c          777 g          427 t
ORIGIN      
        1 gagatggcca tgatgagcgg catcaggatg ccgcccggta tgtagagcag gcgcacgaac
       61 agcccgatca gcggcaggtt gagtatcaac agcatgatgt tgccgacata catgctgtcg
      121 atgaggcccc acaccagatc cgggcggttc tggaacagca gcggtcccgg ctgaatgccg
      181 tacatgatga acgcgccgag catcacggcg gtcgcgccgc ctccgggtat gccgagcgtc
      241 aacagcggca ccatcgcgcc ggcggtgtcc gaattgttcg ccgcctcggg tcccgctacg
      301 ccctcgatgg caccgtttcc gaattcctcg gggtgcttgg acaaccgctt ctcccagatg
      361 taggtcatga tcgaggcgat agtcccgccg gcgcccggca acacgccgat gacgaaaccg
      421 atgacgctgc cgcgcatgat cggcatcagc gagcggtccc actcattctt cgtgaaccag
      481 agcttgcctt tcaggcggat gatctccggt cgcgtgcctt tcgtcagttc ctccatcgtc
      541 gagaatacct cggcgatcgc gaacagcccg accaccgcga tcacgaagcc gactccgtcc
      601 tgcagctccg ggacacccat ggtgtagcgc ggctgcccgc tttgcaggtc gatgccgatc
      661 gtcgcaatca tcaggccgag gacggtcgag atcatcgcct tggcgggcga tctgccggcg
      721 agcgaggaaa ccgcggtcat ggcgaagaac atcagcatga agtattcggc ggggccgaac
      781 ttgagcgcca tgctcgtgag cgggatggcg aacagggtga gtccgatcac gcccagcgtg
      841 ccggcgatga acgatcccac cgccgatatc gcgagcgcgg ccccggcgcg acccttcttc
      901 gccatctggt agccgtcgat cgcggtcatc accgacgcgg cctcgccggg cgtattgatc
      961 aggattgccg tggtggaacc gccgtacttg gtgccgtaat agatgcccgc catcatgatc
     1021 agcgcggagg tcggattcat gccgtaggtc accgggatca gcagcgcgat gcccgccgac
     1081 ggcccgatgc cgggcaggac gccgatgatg gtgccgagga aaacgccaag gaacgtgtac
     1141 cagagattga tcggctgcag gcagaccgcg aaaccgttga ggatatgggt cagtgtttcc
     1201 attcgcagcc tacttgagga atccgggcac cgacttgaac aggttgacga cggaattggt
     1261 cgccacgatg acggcgtcgc ttatcccgct gatgccctcc accccgcgcg gcagattgac
     1321 atcgagcttg acgaacatga tgtagctcag cacgctgtag agcactgcgg tcagcccgtt
     1381 cgcgacccac ttgccgcgat tgaaccaggc catcagtgcg aacaggtaaa tggtggaatc
     1441 gacgatgtac cccagcttct cgagcaaagc gtagtacacc ccggtccaga cgacgacccc
     1501 cgccaggatc cagagatgac ggacatcccg cggcgcagcc gcggcggggg ccggcgcctg
     1561 cagcttgcgg tccttccaca tctcgacgaa caacagcccg gccgcgatca gcaagccgat
     1621 accgagcagg cgcggaaaag ccttgggacc cagcgggtca ccgatttcga gcgagggtat
     1681 cagcgtcgtg gcgtagaggt acaccgccgc gatgacgatg gtgcaggcga agatgacccg
     1741 gtcggtcatc atgatgggat gctcctcctc aggcggctca tcgttcgagt tgtcacggat
     1801 ctgcagtcgc cgcgcccgcg tcacatgccg ggccacatct tacccaagaa cacggccgac
     1861 tagaagctag cagcgcccct ccccgggccc gttttccaca tattggagtg cgctatcccc
     1921 ctgacgcgtc gcaatatata attcccatcc cttgctggcg gcccggtgcg ccgccacaac
     1981 gtgaaccgtc cagttcggcc ctgtgcgctg gcggcacaac aaaatccagg agcctaaatg
     2041 agccacaacc tgttcaactc cctgcaggaa ttcaagtacg gcggcaaggc cggcaaatat
     2101 tattcgctgc ccgcgctgga aaaagccggg ctcggcaaga tttcacgcct gccggtgtcg
     2161 ttccgcgtcg tgctcgaatc cgccctgcgc aactgcgacg gcaagaagat taccgaagcg
     2221 catttgcgca gcatggccgc gtggaagccc aaggccaagc gcagccagga agtgccgctg
     2281 gtcgtcgcgc gcgtgctgct gcaggacatg accggcgtgc cgctggtggt ggacttcgcc
     2341 gccatgcgcg aagccgcaaa agccatgggc aaggaccccg agatcatcga gccgctctgc
     2401 cccggccacc tcatcatgga ccactcggtg cagatcgata actacggcac ccccgacgcg
     2461 ctccgtaaaa acatggagat cgagttcaaa aggaaccgcg agcgctacca gttcctgaaa
     2521 tggagcgcgg gcgcgttc
//
LOCUS       DBNGSG010000254         4299 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_759274, whole genome shotgun sequence.
ACCESSION   DBNGSG010000254 DBNGSG010000000
VERSION     DBNGSG010000254.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_759274"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="AC158Q_06615"
     CDS             complement(<1..775)
                     /locus_tag="AC158Q_06615"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3339483.1"
                     /translation="MPSAATLPAWYVVLVTTAAQMVVAMANIVLPTIAPKVAETLQVD
                     PVLVGYHVGLTFGAATLTSVYGGLAVMRWGAASATQFAMLSAVAGLGMLALPHLGFIA
                     LGSIAVGIGMGLAAPAAAHLLVRHTPPERRNLVFSIKQTGVPLGGVIVSLTAPALAVT
                     VGWQWALAMIAVAGVATLLLTRRASALWDADRNAAGGLHSNPFGGVPLVWKLEPLRWI
                     SLAAVLFSAVQRILLSFTVIYLVAEGGYGLVEAGVMLSLA"
     gene            1027..1752
                     /locus_tag="AC158Q_06620"
     CDS             1027..1752
                     /locus_tag="AC158Q_06620"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR3339484.1"
                     /translation="MTHPPAGKPLADVRSMPSLSDRAYERIRHDIITCAIAPGTEVSE
                     AQLCAHFRLGKAPVRMALNRLAHDGLVRAIPRRGYRVTAITIRDIHDVFELRLMLEPA
                     AARMAAGRVDAPRLRALDEVCRAGYLPGDTKSTSRFLEANKAFHVEIARSAGNSRLAD
                     AVEQLLDEMTRLLHLGLGPRNRSLEMQREHRALVKALERGDSGTAERICREQIEAARG
                     MVLSAILTSRSVMNLALGAEGPR"
     gene            1749..2261
                     /locus_tag="AC158Q_06625"
     CDS             1749..2261
                     /locus_tag="AC158Q_06625"
                     /EC_number="1.13.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF017778.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HIR3339485.1"
                     /translation="MNVAPQLQRLAGRVAAASGLAPDDMAHEIKAALHEAAAETGWLP
                     PERRRASHEHYARHLVHADPEGRFSILAIVWDRGQMSPIHAHHCWCAVGVYQGLLTET
                     FYRAGAAGEPPVEIGSARHGAGASTFDPGGSGIHRIANYSGVLAVSLHIYGVAGDRIA
                     TGVNRVYSTA"
     gene            2368..3375
                     /locus_tag="AC158Q_06630"
     CDS             2368..3375
                     /locus_tag="AC158Q_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015038776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR3339486.1"
                     /translation="MKAVVLGKHGGLEELQYVKDFPDPKVSDGHVVIRVGATSFNYHD
                     VFTVRGMPGIKVPMPMIIGLDLAGEILETGRGVDPKWKKGDRVLVNPLNREKGLMGEM
                     MHGGLAEKCLVAEHQLIRMPEDVSFEEAASLPVAYGTAHRMLITHKTVNSGDKVLVLG
                     ASGGVGTGCVLLAKMLGAEVIACASSDDKIQRLKALGADHVINYKETDFSKWAIEKFG
                     KPQRRSYEGGVDVVINFTGGDTWAPTLRCVKRGGKILVCGATAGYDPKEDLRYIWSFE
                     LQVIGSNSYYDDNLQALLGLIQEGRMKPVIDKVLPLEKAAEGLRLIENREVFGKVVVT
                     P"
     gene            3533..>4299
                     /locus_tag="AC158Q_06635"
     CDS             3533..>4299
                     /locus_tag="AC158Q_06635"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR3339487.1"
                     /translation="MTVPRPEPRGYPGNLGLLFAGHANSDRPAIIELRDPARPRTVSF
                     RELDAGCNAVARGLARDGLKPGDRVGILSLNRAEFVMTLLGAMRAGVVPVPVNVKLPA
                     DQISFILDDAGARRVFTEPGLRRHCPPHLPIIEFGGNFDSFLDLGSFKAYEPGPNSIA
                     IQPYTSGSTGRPKGVLLSHFGQNWSRRILAHTRGTTERDVILVAAPLYHKNALNAIKQ
                     GLTAGATLPLLPQFNVERYIEAIGHYRCTVISGVPTM"
BASE COUNT          792 a         1418 c         1393 g          696 t
ORIGIN      
        1 gcgcgagaga cagcatgacc cccgcctcca ccaggccgta gccgccttcc gcgacgagat
       61 aaatcacggt gaaggagagc aggatgcgct ggacggcgga gaacagcacc gcggcgagtg
      121 aaatccaccg cagcggctcc agcttccaca ccagcggcac gccgccgaaa gggttgctgt
      181 gcaggccgcc cgcggcattg cggtcggcgt cccagagggc gctggcgcgc cgcgtcagca
      241 gcagcgtcgc cacaccggcc acggcgatca tggcaagcgc ccactgccag ccgacggtga
      301 cggcgagggc cggggccgtc agcgacacga tcacgccgcc gagcggaaca ccggtctgct
      361 tgatcgagaa caccaggttc cggcgctccg gcggggtgtg cctcaccagc aggtgcgccg
      421 ccgccggtgc cgccagtccc atgccgatgc cgaccgcgat cgatcccagc gcgatgaatc
      481 ccaggtgcgg cagcgccagc attcccaggc cggcgacggc ggacagcatg gcgaactggg
      541 tcgccgatgc ggcgccccag cgcatcaccg caaggccgcc gtaaacggag gtcagcgtcg
      601 ccgcaccgaa cgtcaaaccc acgtgatagc cgaccaggac gggatcgacc tgcagcgttt
      661 cggccacctt gggagcgatg gtgggcagca cgatgttcgc catcgccacc accatctgcg
      721 ccgcggtcgt caccagcacg acgtaccagg cgggtaaagt ggcggcggaa ggcatggcgg
      781 cgtttatcca ggggaaggtt cgcgggcgcc gagcggcggc cctctgcgaa ccagggcaac
      841 ctgtctcgga tggtgcccgc tcgaacgcgc gcagttcagc ctgcgcgggg catccggaat
      901 tgacgaatgt tagcagcgaa actgcgcgcg cgttcccgct tcgtccagac tgtggtccgg
      961 tgccgggttt tgacatgtca cggccgcatt catagaattt caacaggctg ccttttcgcc
     1021 ctgcccatga cccaccctcc cgccggaaaa ccgctcgctg acgtccgctc gatgccctcg
     1081 ctgtcggacc gggcttacga acgtatccgg cacgacatca tcacctgcgc catcgcgccc
     1141 gggaccgagg tgtcggaggc gcagttgtgc gcgcacttca ggctcggcaa ggcgccggtg
     1201 cgcatggcgt tgaaccgcct cgcgcacgat ggcctggtgc gcgccattcc gcgccgcggc
     1261 taccgggtga cggcgatcac gatcagggac atccatgacg tgttcgagtt gcgcctcatg
     1321 ctcgagcccg ccgcggcgcg catggcggcc gggcgggtgg acgcgccgcg gctgcgcgcc
     1381 ctcgacgaag tctgccgcgc cggctacctg ccgggagaca ccaagagcac gtcgcgcttt
     1441 ctcgaggcga acaaggcgtt ccacgtggaa atcgcccggt cggccggcaa ttcgcggctc
     1501 gccgacgcgg tggagcagtt gctcgacgag atgacccgcc tgctgcacct gggcctgggc
     1561 ccgcgcaacc gttccctgga aatgcagcgc gagcatcgcg cgctggtaaa agcactcgag
     1621 cgcggcgaca gcggcaccgc cgagcgcatc tgccgcgagc agatcgaggc ggcgcgcggc
     1681 atggtgctgt cggcgatcct gacttcacgc tcggtgatga atcttgcgct cggagcggaa
     1741 ggaccgcgat gaacgtggcc ccgcaattgc agcgcctcgc aggccgcgtt gccgccgcgt
     1801 ccgggcttgc gccggacgac atggcccatg aaatcaaggc ggcgctgcac gaagcggccg
     1861 cggaaaccgg ctggctcccc cctgaacggc gccgggccag ccacgagcat tacgcccgcc
     1921 acctggtcca cgccgatccc gagggccgct tctcgatcct tgccatcgtc tgggaccgcg
     1981 gccagatgag ccccatacac gcccatcact gctggtgcgc ggtcggcgtc taccagggtc
     2041 tgctcaccga gacgttctac cgcgcgggcg cggccgggga gccgccggtg gagatcggca
     2101 gcgcccgcca tggcgcgggc gcctcgacct tcgatcccgg cgggtccggg atccaccgca
     2161 tcgccaatta cagcggcgtg ctggccgtct cgctgcatat ctatggggtc gccggcgacc
     2221 gcattgcgac cggcgtcaac cgtgtttact caactgcgtg agtggtgatt ggtgaatggt
     2281 gaatggaccc ggcgcaaatc gaattgacga tctgaatcca atcaccaatc actattcacc
     2341 attcgcgcct ttacagcgga gcacaagatg aaagcagtag tgctgggaaa acatggcggg
     2401 ctcgaagagc tccagtacgt gaaggatttt cccgatccga aggtgagcga cggccatgtc
     2461 gtgatccggg tcggggcgac ctcgttcaat taccacgacg tcttcaccgt gcgcggcatg
     2521 ccgggcatca aggtgccgat gccgatgatc atcgggctcg acctggccgg ggaaatactc
     2581 gagaccgggc gcggcgtgga cccgaagtgg aaaaagggcg accgcgtgct ggtgaacccc
     2641 ctcaaccgcg agaaggggct gatgggcgaa atgatgcacg gcggtctcgc cgagaaatgc
     2701 ctggtcgcgg aacaccagct gatccgcatg cccgaggacg tcagcttcga ggaagccgcc
     2761 tcgctccccg tcgcctacgg cacggcgcac cgcatgttga tcacgcacaa gacggtaaac
     2821 agcggcgaca aggtgctcgt gctcggcgcg agcggcggcg tcggcaccgg ctgcgttctg
     2881 ctcgcaaaga tgctgggggc ggaagtgatc gcctgcgctt ccagcgacga caagatccag
     2941 cggctgaagg cgctcggcgc cgatcatgtc atcaattaca aggaaaccga tttctcgaag
     3001 tgggcgattg agaaattcgg caagccgcag cgccgcagct acgagggcgg cgtggatgtc
     3061 gtgatcaact tcaccggcgg cgacacctgg gcgccgacgc tgcgctgcgt caagcgcggg
     3121 ggcaagatcc tggtgtgcgg cgccaccgcc ggctacgatc ccaaggagga cctgcgctac
     3181 atctggagct tcgagctgca ggtgataggc tcaaacagtt actacgacga caatctgcag
     3241 gcgctgctgg gcctgatcca ggaaggccgc atgaaaccgg tcatcgacaa ggtgctgccg
     3301 ctcgagaagg ccgccgaagg attgcggctg atcgagaacc gcgaggtctt cggcaaggtc
     3361 gtcgtcaccc cgtgaacaac gtaatggaca ggatttacat gattaacaag atttaaacaa
     3421 accccataca cgatcgggtc aagctttctt ggttttttat tcaatcctgt aaatcctgtt
     3481 aatcctgtct attgcttttg acgcttggtt ttcaatcccg tcaatcctgt ctatgactgt
     3541 tccacggcca gaaccgcgcg gctatcccgg caacctcgga ttgttgttcg ccgggcacgc
     3601 gaattccgac cggccggcga tcatcgaact gcgcgacccg gcgcggccac ggacggtgag
     3661 cttccgcgaa ctcgacgcag gatgcaacgc cgtcgccagg ggactcgcgc gggacgggct
     3721 caagccgggc gatcgtgtcg gcatcctctc gctcaaccgc gccgagttcg tcatgacgct
     3781 gctcggcgcg atgcgcgcgg gcgtcgtgcc ggtgccggtc aacgtcaagc tcccggcgga
     3841 tcagatatcg ttcatcctcg atgacgccgg agcacggcgg gtattcaccg agcctggcct
     3901 ccgtcgtcat tgtccgccgc atcttcccat aatcgaattc ggcggaaact tcgactcgtt
     3961 cctcgacctc ggatcattca aggcttacga gcccgggccg aattcgatag cgatccagcc
     4021 ttatacctcc ggttcaaccg ggcggccgaa gggcgtgttg ctgtcgcact ttggtcagaa
     4081 ctggagccgg cgcatactcg cgcacacgcg cggcacgacc gagcgggacg tgatcctggt
     4141 cgccgccccg ctctatcaca agaacgcgct gaatgcgatc aagcaggggc tcaccgcggg
     4201 ggcgacgctg ccgctgctgc ctcagttcaa cgttgagcgc tacatcgaag cgatcggaca
     4261 ctaccgctgc accgtcatct ccggcgtgcc gacgatgat
//
LOCUS       DBNGSG010000255         8533 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2965770, whole genome shotgun sequence.
ACCESSION   DBNGSG010000255 DBNGSG010000000
VERSION     DBNGSG010000255.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 8533)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8533)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8533
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2965770"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..1161)
                     /gene="hflX"
                     /locus_tag="AC158Q_06640"
     CDS             complement(34..1161)
                     /gene="hflX"
                     /locus_tag="AC158Q_06640"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HIR3339488.1"
                     /translation="MLDRPANGTAAVLVGLDLGEAGYAESLEEIRLLADSAGIATRAL
                     IKGRRARPDPALFAGKGKVEEVTRALADTGASLAIFNHELSPAQERNLERATGCRVVD
                     RTSLILDIFAQRARSHEGKLQVELAQLEHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRQLLGKRVRVLREKLAKVRSQRAVQRRARGRSNLLAVSLVGYTNAGKSTLFNRMT
                     RAGVYAADQLFATLDTTTRRLHSENGTGIVISDTVGFIRRLPHTLVAAFRATLEETIQ
                     ADLLLHVVDAASEDRDAQIAAVNVVLAEIGADAIPQVLVMNKIDRTPLPARVERDEYG
                     RISRVWASAQSGQGVEFIRLALEESAAAAAASAERNSPAVA"
     gene            complement(1182..1418)
                     /gene="hfq"
                     /locus_tag="AC158Q_06645"
     CDS             complement(1182..1418)
                     /gene="hfq"
                     /locus_tag="AC158Q_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018574547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HIR3339489.1"
                     /translation="MSNKGQLLQDPFLNTLRKEHIPVSIYLVNGIKLQGQIDSFDQYV
                     VLLKNTVTQMVYKHAISTVVPARPVTIPAESTDG"
     gene            complement(1487..2827)
                     /gene="der"
                     /locus_tag="AC158Q_06650"
     CDS             complement(1487..2827)
                     /gene="der"
                     /locus_tag="AC158Q_06650"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013698242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HIR3339490.1"
                     /translation="MLPIIAIVGRPNVGKSTLFNRLTRSRDAIVANVPGLTRDRHYGR
                     GRVGSKAYLAVDTGGFEPVATDGIMHQMARQARQAVDEADAVLFLVDARAGLVPQDRA
                     VAEELRRSGRRVFLVVNKAEGMAPGIAGADFHALGMGDPLPVSAEHGDSVSDLMDLVL
                     ADVPSPKEAKGEDDRHPRIAIVGRPNVGKSTLVNALLGEERVVVYDQPGTTRDSIHIE
                     FERGGRRYTLIDTAGLRRRGRVADATEKFSVMKTLQAIDDANVAILVLDARQDISEQD
                     AHVAGFILEAGRAMVVAVNKWERLPEEERDQVKRALGRKLEFLGFARLHFVSALTGHG
                     LAPLMKSVDEAYASAMARLPTPRLSRALAAAVAQQAPPRAGVLRPKLRYAHQGGVNPP
                     LIIVHGNALERVPETYRRYLERFFREAFKLQGTPLRVSFKTGSNPYADKARSRR"
     gene            complement(3010..4164)
                     /gene="bamB"
                     /locus_tag="AC158Q_06655"
                     /gene_synonym="yfgL"
     CDS             complement(3010..4164)
                     /gene="bamB"
                     /locus_tag="AC158Q_06655"
                     /gene_synonym="yfgL"
                     /inference="COORDINATES: protein motif:HMM:TIGR03300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HIR3339491.1"
                     /translation="MSLRLSLALAALLLGACQTISDGYDRLFGGRPGPKPAALIPIQP
                     KAALRVLWQGSVGSAEKNVFFPWKSGNVVYAVGAAGNVTGFDIARGTPVARAEAGQRV
                     SGGVGAGEGLVLLGTAKGEVLAFDRSGKAMWKTQVSGEVLAPPQAQEGIVVARAGDGR
                     VFGLEAASGKQKWVYQRSTPALSVRTHAGFVIERGAVFVGFPGGRLVGIALANGKVGW
                     DAVVALPRGSTELERVADITSLPVIDGQSVCAVAYQGRVACFDAVRGNLIWAREVSSA
                     AGLSVDERYLYVTDDKNAVMALDKGRGASLWRQDKLAGRGVSAPLAIGPYVAVGDFEG
                     YVHLLSREDGSFAARIATDGSAILAPPLALDLTSFLVQTRNGGVFAIIIQ"
     gene            complement(4161..4796)
                     /locus_tag="AC158Q_06660"
     CDS             complement(4161..4796)
                     /locus_tag="AC158Q_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0044877 - protein-containing complex
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HIR3339492.1"
                     /translation="MAYDLEEQEKLDELKSWWAEYGTLVLGAVAACALTIAGFQGWRY
                     YQHSQAIAAVTLYGQLDQAERAGDHKKVRDIAGQITGQYGTTIYAAFAALGSARASFE
                     SGDLAGAKSQLQWVMDNAKEGEIRDIARLRLAGVLLDEKNHAEALKLVDAKPADSLLG
                     LYADLKGDILLAQGKKAEARNAYQLALDKSEAGSSYRSIIQMKLDALGEAT"
     gene            complement(4898..6160)
                     /gene="hisS"
                     /locus_tag="AC158Q_06665"
     CDS             complement(4898..6160)
                     /gene="hisS"
                     /locus_tag="AC158Q_06665"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HIR3339493.1"
                     /translation="MALKTIQAIRGMNDVLPDETPLWESLEESVREWLRAYGYRNIRT
                     PVLEQAELFVRSIGAATDIVEKEMYTFTDGLNGESLALRPEGTAACVRAVIEHNLLYN
                     GPQRLWYWGPMFRHERPQKGRYRQFHQVGVEAIGFSGPDIDAEHLVMCARLWRSLGIE
                     GVTLDLNNLGDAAARARYRSRLVKYLERHEQSLDADSRRRMSTNPLRVLDSKNPAMQE
                     ILEGAPRLADDLDDASGSSFESLQQLLRAAKLEFRINPRLVRGLDYYNGAVYEWVSGN
                     LGAQNAVCAGGRYDGLIEQVGGKPAPACGFAMGIERLLQLMKAAGRSPASAADVYLVN
                     QGEHAQRFAFLAAEQLRDGGVTVTLDVGGASFKSQMKKADARGARFAVVIGDDEAAAG
                     VVGVKPLREDREQKRVSIAEAIALIHKA"
     gene            complement(6287..7570)
                     /gene="ispG"
                     /locus_tag="AC158Q_06670"
                     /gene_synonym="gcpE"
     CDS             complement(6287..7570)
                     /gene="ispG"
                     /locus_tag="AC158Q_06670"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HIR3339494.1"
                     /translation="MKCYPEHGLAPRRGSRQVRVGHVAIGGGAPIVVQSMTNTDTADI
                     TATVRQVAELARAGSEIVRVTVNNSDAAAAVPAIRDQLDRMGINVPVVGDFHFNGHRL
                     LARHPACAEALAKLRINPGNVGRGSKRDEQFATMIEFACRYGKPVRIGVNWGSLDPEL
                     LARMMDDNARLPQPEDAAVVTRKALVVSALESAQQAEALGLPRDRIVLSCKVSGVQDL
                     ISVYRDLASRCDYALHLGLTEAGMGSKGIVASTAAMAVLLQEGIGDTIRVSLTPEPGG
                     DRTREVVVAQEMLQTMGLRSFAPMVIACPGCGRTTSTYFQELADRIQSWLRAQMPQWR
                     ERYPGVENMHVAVMGCVVNGPGESKHANVGISLPGSGENPVAPVYVDGAKTVTLKGED
                     SATDFQRIVEQYVADTYSRDAAAGKKNPAKDAAHA"
     gene            complement(7567..8439)
                     /locus_tag="AC158Q_06675"
     CDS             complement(7567..8439)
                     /locus_tag="AC158Q_06675"
                     /inference="COORDINATES: protein motif:HMM:NF024855.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HIR3339495.1"
                     /translation="MNDAHNSEALAGAGPAHTAPGGRLAEARRVQNLTPADVARQLKL
                     SVWQVDALEAGRYQQLPGPIFVRGFIRNYARLVKLDPNELLRAAGDSIPQAAARPEMP
                     PSQDIPFPAGNRTRWPELAGAAAILVGLLAAYEFYWNEPEAPVTPAIAVSPVPTARPL
                     KNEGPRAAPQPGGEKRAVVPGITVETATQAAPQALPAAGGSQSGQEDSPTPGERQISL
                     VFDQESWVEIRDRNNKVVFSQLNRAGTQQSLSGLPPLSVVVGNSHGVRMRYDDQPVDL
                     ARHTRVDVARLILQ"
BASE COUNT         1355 a         2929 c         2706 g         1543 t
ORIGIN      
        1 tacgtaactg atcatttcag gttagggtgc gtcttacgcc acggcggggc tattgcgttc
       61 tgcggatgcc gcagcagcag cggcgctttc ctcgagtgcc aggcgtatga attcgacgcc
      121 ctgtcccgat tgtgcgctcg cccagacccg agaaattcta ccatactcgt cgcgctcgac
      181 ccgggccggc agcggcgtgc ggtcgatctt gttcatcacc aggacctgcg ggatcgcgtc
      241 cgccccgatt tcggccagga ctacgttgac cgccgcgatc tgcgcgtcac gatcctcgct
      301 cgccgcgtcc accacgtgca gcagcaggtc ggcctggatg gtctcctcca gcgtcgcccg
      361 gaacgccgcg accagggtgt gcggcagccg ccggatgaaa ccaacggtat ccgaaatgac
      421 gatgccggtg ccgttttcac tgtgcagccg ccgggtcgtg gtatcgagcg tggcgaacag
      481 ctggtcggcg gcatagacgc cggcgcgggt catgcgattg aacagcgtgg acttgccggc
      541 attggtgtag cccaccagcg agaccgccag cagattggag cggccgcgag cgcgccgctg
      601 caccgcgcgc tgggagcgga ccttggccag cttctccctc agcaccctga cccgcttgcc
      661 gagcagctgc cgatcggttt cgagctgggt ttcgcccggt ccgcgcaacc cgatgccccc
      721 cttctgccgc tccaggtggg tccagccccg caccagccgg gtcgccagat gctcgagctg
      781 cgctagctcg acctgcagct tgccctcgtg actgcgggcg cgctgggcaa agatatcgag
      841 tatcaggctg gtgcggtcga cgacacggca gccggttgcg cgctcgaggt tgcgttcctg
      901 cgccggcgac agctcgtggt tgaagatcgc aaggctcgcg ccggtatcgg ccagcgcccg
      961 cgtcacctct tccactttgc ccttgccggc aaagagcgcc gggtcgggcc gggcgcggcg
     1021 acccttgatc agggcgcggg tggcgatgcc ggcactgtcg gcgagcaggc ggatctcttc
     1081 caggctctcc gcatagccgg cttcgcccag gtcgagaccg accaggaccg ccgccgtgcc
     1141 gttcgcggga cggtcgagca tctagcggga aaagacagcc atcagccgtc tgtgctctcg
     1201 gccgggatag tgacgggacg cgcaggcacc acggtcgaga tggcgtgctt gtagaccatc
     1261 tgggtcaccg tgttcttcag cagcaccacg tactggtcga aggagtcgat ctgtccctgc
     1321 aacttgatgc cgttgaccag gtagatcgat acggggatgt gttccttgcg caatgtgttg
     1381 agaaaggggt cttgtagcag ctgccctttg ttgctcatgt cggcctcgtc ttttggtgtt
     1441 tttgggatgg gtactctact cgttattctg ctgttatgcc atagggttag cggcgcgagc
     1501 gcgccttgtc cgcatacggg ttggagccgg tcttgaagct cactcgcaac ggcgttcctt
     1561 gcagtttgaa agcttcccgg aagaaacgtt ccaggtaacg gcggtaggtt tccggcacgc
     1621 gctccagggc gttgccgtgg acgatgatga gaggtgggtt gacgccgccc tggtgcgcgt
     1681 agcgcagctt ggggcgcagc acgccggcgc gaggcggcgc ctgctgggcc accgctgccg
     1741 ccagcgcgcg gctgaggcgg ggggtgggca ggcgcgccat tgccgatgcg taggcctcgt
     1801 ccaccgactt catcagcggc gcgaggccgt ggccggtcag cgctgaaacg aaatgcagcc
     1861 gcgcgaatcc cagaaactcg agcttgcgcc cgagggcgcg cttgacctgg tcgcgttcct
     1921 cctcgggcaa tcgctcccat ttgttcaccg ccaccaccat cgccctgccc gcctcgagaa
     1981 tgaagccggc gacgtgcgcg tcctgttccg agatgtcctg gcgcgcgtcg agcaccagga
     2041 tggcgacgtt ggcgtcgtcg atcgcctgca gcgtcttcat caccgagaac ttctcggttg
     2101 cgtccgccac ccgcccgcgc cggcgcagcc ccgccgtgtc gatcagcgtg tagcggcgcc
     2161 cgccgcgctc aaattcgatg tgaatcgagt cgcgtgtcgt gccgggctgg tcgtagacga
     2221 cgacgcgctc ctccccgagc agcgcgttga ccagcgtgga cttgccgacg ttgggccggc
     2281 cgacgatcgc gatcctagga tggcggtcat cctcgccctt ggcctccttt ggcgagggaa
     2341 cgtcggccag cacgaggtcc atcaggtcgg agacggaatc gccgtgttcg gccgaaaccg
     2401 gcagcgggtc gcccatcccg agcgcgtgga aatccgctcc ggcgattccc ggcgccatgc
     2461 cttcggcctt gttgacgacc aggaaaacac ggcggccgct gcggcgcagt tcctcggcga
     2521 ccgcgcggtc ctggggaacc agcccggcgc gcgcatccac caggaacagg acggcgtcgg
     2581 cttcgtcaac cgcctggcgc gcctggcgcg ccatttgatg catgatgccg tccgtcgcca
     2641 ccggctcgaa gcctcccgtg tccaccgcga ggtacgcctt gctcccgacg cggccccgcc
     2701 cgtagtgacg gtctcgcgtc aggcccggca cattggcgac gatcgcgtcg cgcgaacggg
     2761 tcagccgatt gaaaagcgtg gacttgccga cattgggccg gccgacgatg gcaataatag
     2821 ggagcatgga aaccggaaat tagccgccaa ggcgccaaca gcgccaagca atacaaaaat
     2881 aaagagaaca acccaggagc tgggacttac gctgagtaac ggtaaccata gcccccgtga
     2941 ttcagaacgg gcgaaaatat tttctgactg gctttccttg gcggccctgg cgtcttggcg
     3001 gctattttat tactggataa tgatcgcgaa aacgccgccg ttgcgggtct gcaccaggaa
     3061 gcttgtcagg tcgagcgcca aggggggcgc aagaatagcg ctgccgtcgg ttgcgatgcg
     3121 cgccgcgaaa gagccgtcct cgcgcgaaag caggtgcacg tagccctcga aatcgccgac
     3181 cgcgacgtaa ggcccgatcg ccaggggcgc gctgacgccg cggccggcga gcttgtcctg
     3241 ccgccagaga ctcgcgccgc gccccttgtc gagcgccatc accgcattct tgtcgtcggt
     3301 cacgtacagg taacgctcgt ccacgctcaa cccggccgcg ctggagacct cccgcgccca
     3361 tatgaggttg ccgcggacgg cgtcgaagca ggctacccgg ccctggtagg ccaccgcgca
     3421 cacgctttgc ccgtcgatca ccggcaggct ggtgatatcg gcaacgcgct ccagctcggt
     3481 cgacccgcgc ggcagcgcca ccaccgcgtc ccagccgact ttgccattgg caagcgcgat
     3541 gccgacaagg cgcccgccgg gaaacccgac aaataccgcg ccgcgctcga tcacgaagcc
     3601 ggcatgcgtg cgcaccgaga gcgccggcgt ggagcgttga tacacccatt tttgcttgcc
     3661 gctggccgct tccagaccga aaacacggcc gtcgccggcg cgcgcgacta cgatgccttc
     3721 ctgcgcctgc ggtggcgcaa gcacttcacc tgagacctgc gttttccaca tcgctttccc
     3781 gctgcggtcg aatgcgagca cttcgccctt ggcggtaccg agcagcacca ggccctcccc
     3841 ggcgccgacg ccgccggaga cgcgctggcc ggcctcggcg cgcgccacgg gtgtgccgcg
     3901 cgcgatgtcg aaaccggtga cgttgcccgc cgcgccgacg gcataaacca cgtttccgga
     3961 tttccatgga aaaaagacgt ttttctccgc tgatcccacg ctgccctgcc acagcaccct
     4021 cagcgcggct ttgggctgta tcggtatgag cgcggccggc ttcgggcccg ggcgaccgcc
     4081 gaacaggcgg tcatagccgt cggagatcgt ctggcaggcg cccagcagca gcgccgccag
     4141 cgccaggctc aagcgcaggc tcacgtggct tcgcccagcg cgtcgagctt catctggatg
     4201 atggaacgat aggagctgcc cgcctcgctc ttgtccagcg cgagctggta ggcgttgcgc
     4261 gcttccgcct tcttgccctg cgcgagcagg atgtccccct tgagatcggc ataaagcccg
     4321 agcagggaat cggccggctt ggcgtccacc agcttcagcg cctcagcatg gtttttctcg
     4381 tcgagcagca cgccggcgag ccgcaggcgc gcgatgtccc ggatctcgcc ctccttcgcg
     4441 ttgtccatca cccactgcaa ctgggatttg gcaccggcga ggtccccgga ctcgaaactg
     4501 gcgcgggcgc tccccagggc agcgaacgcg gcgtagatcg tcgtcccgta ctggccggtg
     4561 atctggcctg caatatcgcg caccttcttg tggtcaccgg cgcgctcggc ctgatcgagc
     4621 tgaccgtaga gcgtcaccgc cgctatcgcc tggctgtgct ggtaatagcg ccagccctga
     4681 aatcccgcga tggtgagcgc gcaggcggca acggccccca gcaccagcgt gccgtattcc
     4741 gcccaccacg acttgagctc gtccagcttt tcctgttctt cgagatcgta tgccatttag
     4801 tttccttgac tgcgtgattc gtgaatggtg aatcgtgatt caaaaaaaca gcgctcacgg
     4861 actttcccgt cacgaagcac aactcacgaa tcaccattca cgccttatgt atcagagcga
     4921 tcgcttccgc tatcgaaacg cgtttttgtt cccggtcctc gcgcagcggt ttcacgccga
     4981 caacgcccgc tgcagcctcg tcgtcgccga tgacgacggc gaagcgcgcg ccgcgtgcgt
     5041 ccgccttttt catctgggac ttgaagctgg cgccgcccac gtcgagcgtc actgtgaccc
     5101 cgccgtcgcg cagctgctcg gccgccagga acgcaaaccg ctgcgcgtgc tccccctgat
     5161 tcaccagata gacatccgcg gcggacgccg gtgaccgccc ggcggctttc atcaactgga
     5221 gtagccgctc gatccccatg gcgaacccgc aagcgggcgc gggcttgcct ccgacctgct
     5281 cgatcagccc gtcataacgg ccgcccgcgc ataccgcgtt ctgtgcaccc agatttccgc
     5341 ttacccactc gtacaccgcg ccgttgtagt aatccaggcc gcgcaccagc cgtggattga
     5401 tgcgaaactc cagtttcgcg gcccgcaaca attgctgcag gctctcgaag ctgctccccg
     5461 atgcatcgtc caggtcgtcg gcgagtcttg gcgcgccctc gaggatttcc tgcatggccg
     5521 ggttcttgct gtcgagtacg cgcagggggt tcgtgctcat ccgcctccgg gaatcggcat
     5581 cgagagactg ttcgtggcgt tccaggtatt tcaccaggcg cgaacggtaa cgggcccgtg
     5641 ccgccgcgtc gccaaggttg ttgagatcga gcgttacgcc ttcgatgccc aggctgcgcc
     5701 acagccgggc gcacatcacc aggtgctcgg cgtcaatgtc ggggcccgaa aacccgatcg
     5761 cctccacccc gacctggtga aactggcggt agcgcccttt ttgtggccgc tcgtgacgga
     5821 acatcggccc ccagtaccac aggcgttgcg ggccgttgta gagcaggttg tgctcgatca
     5881 ccgcgcgcac gcatgccgcc gttccctccg ggcgcagcgc caggctctcg ccgttcaatc
     5941 cgtcggtgaa agtgtacatc tccttttcga cgatatcggt tgctgctccg atcgaacgca
     6001 cgaacagctc ggcctgctcc aggaccggcg tgcggatatt gcgataaccg taggcgcgca
     6061 gccactcgcg taccgactcc tcgagcgact cccacagcgg ggtctcgtcc ggcaggacat
     6121 cgttcatccc gcggattgcc tgtatcgttt ttagagccat tgcgatcggg gccggttatt
     6181 cgcagcgtat cgttttatcc cggaactgcg agaatcgtga ttcgaacaga cacctcttca
     6241 ctcaccaatc accgctcgct actcgcgaat cacgcatttc ggggagtcag gcgtgcgcgg
     6301 cgtccttcgc cggatttttc ttccccgcag cagcgtctcg tgaataggta tccgccacgt
     6361 actgctcgac gattctctgg aagtccgtgg cactgtcctc gcccttcaac gtcacggtct
     6421 tggcgccgtc gacgtacacc ggcgcgaccg ggttttcgcc cgacccgggc aggctgatcc
     6481 cgacgttggc gtgcttgctc tcgcccggcc cgttcaccac gcagcccatc accgcgacgt
     6541 gcatgttctc cacgcccggg tagcgctccc gccactgcgg catctgcgcg cgcagccagc
     6601 tctggatgcg gtcggccagt tcctgaaaat aagtgctggt ggtgcgcccg caacccgggc
     6661 aggcgatcac catcggcgcg aacgaccgca accccatggt ctgcagcatc tcctgcgcca
     6721 ccaccacttc gcgggtacgg tctccgcccg gctccggcgt gagcgaaaca cggatcgtgt
     6781 ccccgatacc ctcctgcagc agcaccgcca tcgccgcggt ggaggcgacg atgcccttcg
     6841 agcccatgcc ggcctcggtc agccccaggt gcagcgcgta gtcgcagcgg gacgccaggt
     6901 cgcggtagac ggaaatcagg tcctgcaccc cgctcacctt gcacgagagc acgatgcggt
     6961 cgcgcggcaa tccaagtgcc tcggcttgct gtgcgctttc cagcgctgag accaccagtg
     7021 ccttgcgcgt caccaccgcg gcatcctcgg gctgaggcag ccgggcattg tcgtccatca
     7081 tgcgcgcgag tagctccggg tcgagactgc cccaattgac gccgatgcgt accggtttgc
     7141 cgtagcggca ggcgaactcg atcatggtgg cgaactgctc gtcgcgcttc gatccgcgcc
     7201 ccacgttgcc gggattgatg cgcagcttgg ccagcgcttc ggcgcacgcc ggatgccggg
     7261 cgagcaggcg gtgcccgttg aaatgaaaat cgccgacgac cggcacgttg attcccatcc
     7321 tgtcgagctg gtcgcggatt gcgggcactg ccgccgccgc gtcggaattg ttgacggtca
     7381 cgcgcacgat ttcggaaccg gcgcgcgcca gttcagcgac ctggcgcacg gtagcggtga
     7441 tatcggccgt atcggtgttg gtcatggact ggaccacgat cggggcgccg cccccgatcg
     7501 cgacgtggcc cacccgcacc tggcggctgc cgcggcgcgg cgccagtccg tgttccggat
     7561 agcacttcat tgcagtatca ggcgcgcaac gtcgaccctg gtgtggcgcg caaggtcgac
     7621 tggctggtcg tcgtagcgca tccgcacgcc atgcgaattg ccgaccacga ccgacaacgg
     7681 cggcagcccg ctgagggact gctgcgtgcc cgcgcggttg agctgggaga acaccacctt
     7741 gttgttgcgg tcgcggattt ccacccacga ctcctggtcg aagacgaggc tgatctgcct
     7801 ctcccccggc gtgggcgaat cctcctggcc ggattgagag ccgccggccg caggcaacgc
     7861 ctgcggcgcc gcctgagtcg ccgtttccac ggtgatgcct ggtacgacgg cgcgtttttc
     7921 gccgcccggt tgcggagcgg cccgcggccc ttcgttcttc agcggccgcg ccgtcgggac
     7981 cggggaaacg gcgatcgctg gcgtcaccgg cgcctccggc tcgttccagt aaaactcgta
     8041 ggccgcgagc aggcccacga gaatggccgc ggcgccagcc agctcaggcc agcgcgtccg
     8101 gtttccggct ggaaatggaa tgtcctgtga cggcggcatc tccggccgcg ccgcagcttg
     8161 cggaatgctg tcgcccgccg cgcgcagaag ctcgttcggg tcgagcttca ccagccgcgc
     8221 gtaatttcgg atgaatccgc gcacgaagat cggtccgggc aactgctggt agcgcccggc
     8281 ctcgagcgca tccacctgcc acacggaaag cttcagttgg cgggcgacat cggcgggcgt
     8341 caggttttgc acccggcgtg cctcggccag gcgcccgccc ggcgccgtgt gagcggggcc
     8401 ggcgcccgcc agcgcctcac tgttgtgggc gtcgttcatt cgtaccggcc cgcgctcagc
     8461 gcgcgcgcct ccgcggaatt cggataccga ttgcgcaatt gcgacgcata gctcgcctcc
     8521 gagttgcgat cgc
//
LOCUS       DBNGSG010000256         1113 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4494341, whole genome shotgun sequence.
ACCESSION   DBNGSG010000256 DBNGSG010000000
VERSION     DBNGSG010000256.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4494341"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="AC158Q_06680"
     CDS             complement(<1..184)
                     /locus_tag="AC158Q_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339496.1"
                     /translation="MLVATVLGAPAVCWASGLPWILVDTEALTLTVFSAENRVLARFQ
                     DISIGSGGAAKIHRRGD"
     gene            224..>1113
                     /locus_tag="AC158Q_06685"
     CDS             224..>1113
                     /locus_tag="AC158Q_06685"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR3339497.1"
                     /translation="MKSMPFFTTPTALRVILGLHILVLLVCASLPARGASFLLPTDGS
                     NIIGQFQVVTADSRNTLLDIARHFDLGSEEITVANPAVSAWLPGEGTPIVVPTVFILP
                     PKPWQGIVLNIPQRRLYYFPRHKANEAATVVTFPIGTARAGWPTPLGTTRITGKYKDP
                     AWFVPKSIQEEHRKQGEFDFPEYFPPGPDNPMGMLAMETGFDKIFIHSTNRPWGVGMR
                     VSHGCIRLYPEDAAYLFPELPVGAPVRIINQPVMVGERDKLLYLSVSKPVGEYPDDVD
                     DLLLRAVDAVALYLEAQPIAP"
BASE COUNT          205 a          389 c          323 g          196 t
ORIGIN      
        1 cgtcgccgcg ccgatggatt ttggctgcac cgccgctgcc gatggaaata tcctggaagc
       61 gtgccagaac gcggttttca gcggaaaaga ccgtcagggt aagcgcctcg gtatcgacca
      121 ggatccaggg cagtccgctt gcccagcaaa ccgcgggggc ccccaggacg gtggcgacga
      181 gcaaggcgac gagcccccgc ttgaggattg ccgcgagctg cccatgaaat ccatgccatt
      241 tttcacgacc ccgacagcgc tgagagtgat tctcggtctg catatcctcg tgctgctcgt
      301 atgtgcgtcg ctccccgccc ggggtgcgag cttccttctg cctaccgatg gcagcaatat
      361 catcggccag ttccaggtgg taaccgccga ttcccgcaac accctgctcg acatcgcccg
      421 tcattttgac ctcggctccg aggaaattac cgtcgccaat cccgctgtct cggcctggct
      481 gcccggcgag ggaaccccga tcgtcgttcc caccgtgttc atactgccgc ccaagccgtg
      541 gcagggtatc gtcctcaaca tcccgcagcg tcgactgtac tatttccccc gccacaaggc
      601 caacgaagcc gcgacggtcg tcaccttccc gatcggcacc gcgcgggccg gctggcccac
      661 gccgctgggc acgacccgca tcaccggcaa gtacaaggac cccgcctggt tcgtcccgaa
      721 gagcatccag gaagagcacc gtaaacaggg cgagttcgac ttcccggaat acttcccgcc
      781 cggccccgac aaccccatgg gcatgctcgc catggaaacc ggattcgaca agatcttcat
      841 tcatagcacc aaccgaccct ggggggtcgg gatgcgcgtg agccatggct gcatccgcct
      901 gtatcccgag gacgccgcct acctttttcc ggaactgccc gtcggcgcgc cggtgagaat
      961 catcaatcaa ccggtgatgg taggcgagcg tgacaagctt ctctacctca gcgtgtccaa
     1021 gccggtgggc gagtacccgg acgacgtcga cgacctgctt ctgcgggcgg tcgacgcggt
     1081 ggcgctctac ctggaggcgc aaccgatcgc gcc
//
LOCUS       DBNGSG010000257         4362 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1099302, whole genome shotgun sequence.
ACCESSION   DBNGSG010000257 DBNGSG010000000
VERSION     DBNGSG010000257.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4362)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4362)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4362
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1099302"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="AC158Q_06690"
     CDS             complement(<1..532)
                     /locus_tag="AC158Q_06690"
                     /inference="COORDINATES: protein motif:HMM:NF014073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR3339498.1"
                     /translation="MVETFNNLMLGFSVALQPSILVYAFVGCLIGTLVGMMPGLGPLA
                     GISLLLPATFGLNPIVAIVLLSGVYYGAMYGGSTTSILMRIPGEAASVMTCVDGYAMT
                     QNGRAGPALAIAAIGSFIAGTLGVVGLMLLAPTLAEFALRFGPPEYTALLLMGLFILA
                     YMSGGSMLKTLAMAAFG"
     gene            complement(620..1093)
                     /locus_tag="AC158Q_06695"
     CDS             complement(620..1093)
                     /locus_tag="AC158Q_06695"
                     /inference="COORDINATES: protein motif:HMM:NF018979.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HIR3339499.1"
                     /translation="MTESPGSGLRGLRSDQFSGLMLLALALYVAWENRAYPLGTLQEP
                     GPGYLPLLLAIFLGSLGLLVALWGMKSAPLASVRWTESKRAIIILVACLVATFALERI
                     GYRLTVIALLVFFLGVLERRPPLPVALVSAGFGLVSFYVVGDLLHVPLPRSPWGF"
     gene            complement(1214..2191)
                     /locus_tag="AC158Q_06700"
     CDS             complement(1214..2191)
                     /locus_tag="AC158Q_06700"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3339500.1"
                     /translation="MKIHLGLLAICLLAPLAASAQEAFPNRAITMIVAFPPGGVADIT
                     ARPTAAAMEKILKQPVSVVNRPGAAGAVGNAAVANARPDGYTVLMALSSISVIPAADE
                     LFSRKPAYSLDQFAPIALVSADPTILVVHPSLPVKTLKELLTIARSKPGALSYSSSGI
                     YGALHMPMEMFVHAAKIRMRHVPTNGGGPAITAVLGGHVELTAGGPAAISGHVKSGKM
                     RPIASWGGARHSSYPQIPTFKELGYKDVEYYIWAGMFAPRGTSDAVMKVLRDAASRAV
                     QDPAFKATMAKVDSIVDYKDAPDFQQYWLADAKRLAALVKVVGKVEAKK"
     gene            complement(2245..3222)
                     /locus_tag="AC158Q_06705"
     CDS             complement(2245..3222)
                     /locus_tag="AC158Q_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3339501.1"
                     /translation="MNLSFPVPRAAVLAAVILGCAGVHAQTYPVKPIRVIVPFGTGGP
                     ADIYARYLGQHLQGPLGQPFVVEARPGAGSIIGTDIVAKSPADGYTLLVMSNTHTVNE
                     SLVPKKPFALMKDFVGIAPINYSDLLLVVHPSLPAKSVKELVALAKKNKGGLNYSSSG
                     TGTPYHMAGELFKALAGVDILHVPHKSSGDARTSVMSGQTELMLDAITTMGPIARAGR
                     VRALGTSGKERSKIMPDVPTIAEAGVKDYETTIWLGVMAPAGTPKTILERLNTEITKV
                     TSRPDVQAAWAKQGATPMKMTVAEFEKHLNADIAKWARVVKIAGARADK"
     gene            complement(3393..3953)
                     /locus_tag="AC158Q_06710"
     CDS             complement(3393..3953)
                     /locus_tag="AC158Q_06710"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3339502.1"
                     /translation="MDRVVQKLVAFQRDEIIRHLRHLSPQDRRLRFGAPATDAAIESY
                     AKGIDFARDKVFGIFEHDLALTGVAHLALDVETDTAELGLSVVPEHRGKGYGYALLAR
                     AKLHARNLTYKKLYMHCLAENQVMIHLARKAGMTVVAEFGEADAHVALGDGPLTDYTR
                     EAIHDQIALVDYLFKHHASWLTRKSV"
BASE COUNT          785 a         1413 c         1391 g          773 t
ORIGIN      
        1 ggccgaacgc cgccatcgcc agcgtcttca gcatcgagcc gccgctcatg taggcgagga
       61 tgaacagccc catcagcagc agcgcggtat attccggcgg gccgaagcgc agcgcgaact
      121 cggcgagcgt gggcgcgagc agcatcaggc cgaccacccc cagcgtgccg gcgatgaatg
      181 agccgatggc cgcgatcgcg agcgccggcc cggcgcgccc gttctgcgtc atcgcgtagc
      241 catccacgca ggtcatgacc gacgcggcct ctcccggtat gcgcatgagg atcgacgtgg
      301 tggagccgcc atacatcgct ccgtaataca cgccggagag cagcacgatg gcgacgatcg
      361 ggttcaaccc gaaagtcgcc ggcagcagca ggctgatgcc cgcgagcggt ccgagccccg
      421 gcatcatgcc gaccagcgtg ccgatcaggc agccgacgaa cgcgtagacc aggatcgacg
      481 gctgcaacgc aaccgagaat cccagcatca ggttgttgaa tgtttcaacc atgccgaggg
      541 atgagggatg aggaacaaat gcgaaacttc aaccgctcca gccggctttc tcctccgccc
      601 tccgccctca tccctgctcc tagaagcccc atgggctgcg cggcaatgga acgtggagga
      661 gatcgccgac gacgtagaag gacaccaggc cgaagccagc cgagaccagc gcgaccggca
      721 gcgggggcct gcgctcgagc acgcccagga agaacaccag cagcgcgatc acggtcaacc
      781 ggtagccgat gcgctcgagc gcgaacgtgg cgaccaggca cgccaccagt atgatgatcg
      841 cgcgcttcga ctcggtccac ctgaccgaag cgagcggggc cgacttcatc ccccacaagg
      901 cgaccagcag gcccagggag ccaaggaaga tcgccagcag cagcggcagg tacccggggc
      961 cgggctcctg cagcgtgccg agcgggtacg cgcggttctc ccacgccaca tagagcgcga
     1021 gcgcgagcag catcaacccc gaaaactgat cgctgcgcag gccgcggagg ccggagcctg
     1081 gggactccgt catccggcgc cccgggggaa gaggtatgag agatgaggga tgagggttgg
     1141 gctcgtcact cgtcaccgcc actcttcacc gccactcgtc acccgacact cgacacccga
     1201 cactcgtcac ccgtcacttc ttggcttcga ccttgccgac cactttgacc agcgcggcca
     1261 ggcgcttcgc gtcggcgagc cagtactgct ggaagtcggg ggcgtccttg taatcgacaa
     1321 tcgaatccac cttcgccatg gtggccttga aggccggatc ctgcaccgcc ctgctcgcgg
     1381 cgtcacgcag taccttcatc acggcatcgg acgtgcctct gggcgcgaac atgccggccc
     1441 agatgtagta ttcgacgtcc ttgtagccaa gctccttgaa cgtggggatc tgcgggtacg
     1501 aggagtgccg cgccccgccc cagctggcga tcgggcgcat tttcccggac ttgacatggc
     1561 cgctgatcgc cgccggcccg cccgcggtga gctcgacgtg tccaccgagc accgcggtga
     1621 tcgccggccc accaccattg gtcggcacgt gccgcatcct gattttcgcc gcatgcacga
     1681 acatttccat cggcatgtgc aacgcgccat agatcccgga ggacgagtag gacagcgccc
     1741 ccggtttcga ccgggcgatt gtcagcagct ccttgagcgt cttcaccggc agcgagggat
     1801 gcaccaccag gatggtgggg tcggccgaaa ccagcgcgat cggggcgaac tggtcgagtg
     1861 agtacgccgg cttgcggctg aacagctcgt cggcggcggg aatcacggaa atgctcgaca
     1921 gcgccatgag cacggtgtag ccgtcgggcc tggcgttggc cacggcggcg ttgccgaccg
     1981 cgccggccgc gcccggccgg ttcacgaccg agaccggctg cttgagtatc ttctccatcg
     2041 ccgccgcggt cgggcgggcc gtgatgtcgg ccaccccgcc aggcgggaac gcgacaatca
     2101 tcgtgatggc gcggttcgga aacgcttcct gcgcgctggc cgccagcggc gccagcaggc
     2161 agatggccaa caatcccaaa tgaatcttca tgtttcctcc tcgctgttgc tgtcttttta
     2221 acgcgtcact tgtcactcac cgcattactt gtcggcacgg gccccggcga tcttgaccac
     2281 cctcgcccac ttcgcgatgt cggcattcag gtgcttctcg aattcggcga cggtcatctt
     2341 catcggcgtc gcgccctgct tggcccacgc cgcctggacg tcaggacggc tcgtgacttt
     2401 cgtgatctcc gtgttgagcc tttccaatat cgttttcggg gtcccggccg gagccatgac
     2461 accgagccag atcgtggtct cgtagtcctt cacgcccgcc tccgcgatgg tcggcacgtc
     2521 gggcatgatc ttcgaacgct cctttccgct ggttcccagc gcgcggaccc ggccggccct
     2581 ggcgatcggt cccatcgtgg tgatcgcgtc gagcatcagc tcggtctggc cactcatgac
     2641 gctggtgcgc gcgtcgccgc tgctcttgtg gggaacgtgc aggatatcca ccccggcgag
     2701 cgccttgaac aattcgccag ccatgtggta cggcgtgccg gtgcccgacg acgaatagtt
     2761 gagcccgccc ttgtttttct tcgcaagcgc gaccagctcc ttcactgatt tcgcgggaag
     2821 cgacgggtgc accacgagaa gcaggtctga ataattgatc ggagcgatac cgacaaaatc
     2881 cttcatcagc gcaaacggct ttttcgggac cagcgactcg ttgaccgtat gagtgttgga
     2941 catgaccagc agcgtatagc cgtcggcggg cgacttggcc acgatatcgg tgccgatgat
     3001 ggagccggcg ccgggccggg cttcgacgac gaacggctgg ccgagaggcc cctgcaggtg
     3061 ctggccgaga tagcgggcgt agatatcggc cggtccgccc gtgccgaaag gaacgatgac
     3121 gcgaatgggt ttcacggggt aggtctgcgc gtgaacgcca gcgcacccca gaatgaccgc
     3181 ggccagcacg gcggcgcgtg gcaccgggaa cgaaagattc attggctcct cctggcgggt
     3241 tgaagaataa cgcccccttt atagatatta tcggggcatt gcagaggcag tatccaatcc
     3301 cctgcgggcg gcgtcaaccg ctgcggaatg aggcgtggac cgtgattcgt gagtcgtgat
     3361 tcgacccgct ccggcgcgcc ggtcagcaca tatcagacac tcttgcgggt aagccagctc
     3421 gcatggtgct tgaacaggta atccacgagc gcgatctggt cgtggatggc ttcgcgcgtg
     3481 tagtcggtga gcggcccgtc cccgagcgcc acgtgcgcgt ccgcctcgcc gaactcggcg
     3541 acgacggtca tgccggcctt gcgcgcgagg tggatcatca cctggttctc ggcgagacag
     3601 tgcatgtaga gcttcttgta ggtcaggttt ctggcatgaa gcttggcgcg cgcgagcagc
     3661 gcgtagccgt agcccttgcc gcggtgctcg ggcaccaccg acaggcccag ctcggccgta
     3721 tccgtctcca cgtcgagcgc gaggtgcgcg acgcccgtga gcgcaaggtc gtgctcgaag
     3781 atgccgaaca ccttgtcgcg ggcgaaatcg atgcccttcg catagctctc gatcgcggcg
     3841 tcggtcgcgg gcgcgccgaa acgcaaccgg cgatcctgcg gcgagagatg tcgcaggtgg
     3901 cggatgattt cgtcgcgctg gaatgcaacc agcttctgta cgactctgtc catgcaatga
     3961 gctattgggg gatcgttagc cgcgcagcca taatgctgca ttgcaatata acagatttga
     4021 ctcatggggc acctgaatag ttctaatgta tttcaagtat tttcttacac tgttgtgttt
     4081 ttaaaatatc gcaacgatgc ttcatgtcgc gcattttttg tgcttcgcac cattacgaag
     4141 catggcgtgc caaatgcagt gctggtagct tgcttcgggc tgccaccatc ccgggagacg
     4201 agtcatgctg gataacagaa agccgtaccg ccgtcagttc gtgcggatgg ccgcgacggt
     4261 tgccgcggcc ctcgcgctgg ccggcgcgcc ctgcgcgctg cgagccgccg atgcgcccaa
     4321 gggtttgcgg atcggcgtca tcgggtcggg caacgtcggc gg
//
LOCUS       DBNGSG010000258         2401 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4495203, whole genome shotgun sequence.
ACCESSION   DBNGSG010000258 DBNGSG010000000
VERSION     DBNGSG010000258.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4495203"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="AC158Q_06715"
     CDS             <1..455
                     /locus_tag="AC158Q_06715"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HIR3339503.1"
                     /translation="NAADLKLWTGGAAPALALTDLEGGQHRLADYRGRVVLINFWATW
                     CAPCRDEMPSIQRLKEKLAGKPFAVLAVNLDEPESRIRKFLSGMKLDFTILLDPRRTA
                     ARAWNARVLPASFIIGPDGRVRYTLVGEINWANEHVVARISELLPAGR"
     gene            complement(551..1492)
                     /gene="cysB"
                     /locus_tag="AC158Q_06720"
     CDS             complement(551..1492)
                     /gene="cysB"
                     /locus_tag="AC158Q_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HIR3339504.1"
                     /translation="MKLQQLRYLREVAKRGLNLSEAAETLHTSQPGISKQIRQLEDEL
                     GVDILVRHGKRVVDMTEPGRVILGIAERILQDTENLKQVAREFGAEGTGSFTIATTHT
                     QARYALPPVITRFSQRYPKVRLSLRQGSPKDIAELVRSGEADIAIATEAIEFYDGLVL
                     LPCSQWNRCVITPLRHPLVKEKQISIEAIARHPLITYDFAFTGDSPIKRAFDNKGITP
                     NVVLTAIDADVIKAYVEMGMGIGILARMAFDPARDLGLRLIDASHLFEPSTTRIAMKP
                     NAYLRGYIYDFIELFAPHLKRAVVDATLKGEGSSYEL"
     gene            1601..>2401
                     /locus_tag="AC158Q_06725"
     CDS             1601..>2401
                     /locus_tag="AC158Q_06725"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HIR3339505.1"
                     /translation="MYQYDEIDQRLVDERVAQFRDQTHRFLAGKLSEDDYRPLRLRNG
                     LYIQRYAPMLRVAIPYGLLSSRQLRTLAHIARKWDRGYGHFSTRQNIQYNWPKLEDVP
                     DILAELAKVEMHAIQTSGNSFRNITTDHYAGVARDEIVDSFVWCELLRQWSTFHPEFT
                     YLPRKFKIAVNGAAADRAAVFLHDIGLQALRDDADEVGFKVIVGGGMGRTPIVGHVVR
                     EFLPWRHLFTYVEAVLRVYNRHGRRDNLYKARIKILVKERTPDKFREEV"
BASE COUNT          437 a          734 c          763 g          467 t
ORIGIN      
        1 cgaacgcggc ggatctgaag ctttggacag gcggggctgc gcccgcgctg gcgctgacgg
       61 atctcgaagg gggccagcac cggctggccg actatcgggg cagggtggtc ctgatcaact
      121 tctgggcgac ctggtgcgcg ccgtgccgcg acgagatgcc ttcgatacaa cgcctgaagg
      181 aaaagctggc gggaaagccc tttgcggtgc tggcggtcaa cctggacgag cccgagtcgc
      241 gcatccggaa attcctctcc ggaatgaagc tggatttcac catactgctg gatcccagga
      301 ggacggcggc cagggcctgg aacgcccgcg tcctgccggc gagcttcatc atcgggcccg
      361 acggccgggt ccgctacacc ctggtcggcg agatcaactg ggccaacgag cacgttgtcg
      421 cgcgcatttc ggaactgctg ccggccggcc gctaacggcg tgaatagtga atagtgaatg
      481 gtgattggcg attggaattc gcgcccgcgc gaagcgcgcg caggaaattc tatttaccat
      541 tcacgccttc ttacagctcg tagctcgagc cctcgccctt cagcgtcgca tccaccaccg
      601 cgcgcttcag gtgcggggcg aacaattcga tgaaatcgta gatgtagccg cgcaggtagg
      661 cgttgggctt cattgcgatg cgcgtggtgc tgggctcgaa caggtggctc gcgtcgatca
      721 gcctcaatcc caggtcgcgc gcggggtcga acgccatcct ggccagtatg ccgatgccca
      781 tgcccatctc gacgtaggcc ttgatgacat cggcgtcgat cgccgtcagc accacgttgg
      841 gcgtgatgcc cttgttgtcg aacgcgcgct tgatggggga gtcgccggtg aacgcgaagt
      901 cgtaggtgat cagggggtga cgcgcgatcg cctcgatcga gatctgcttc tccttcacca
      961 gcgggtgcct gagcggcgtg atgacgcagc ggttccactg ggagcacggc agcagaacca
     1021 ggccgtcgta gaactcgatg gcctcggtgg cgatcgcgat atcggcttcg cccgagcgca
     1081 ccagttccgc gatgtccttc gggctgcctt gccgcagcga caaccgcacc ttgggataac
     1141 gctggctgaa gcgggtgatg accggcggca gcgcataccg cgcctgggtg tgagtggtcg
     1201 ctatcgtgaa cgagccggtg ccctctgcgc cgaactcccg cgcgacctgc ttcaggttct
     1261 cggtatcctg caggatgcgc tcggcgatgc cgagtatcac gcgccccggc tcggtcatgt
     1321 ccacgacccg cttgccgtgc cgcaccagga tatccacccc caattcgtcc tccagttggc
     1381 ggatctgctt gctgatgccg ggctgggacg tatggagcgt ttcggcggct tcggacaggt
     1441 ttagcccgcg cttggccacc tcacggagat accttaattg ctgtagtttc atggagatat
     1501 cattaatact ttatagttat aaaagtctaa cacaatattc ttttgtagta taacaatccg
     1561 ttgctaccat gcgagcctcg gatttgattg ggaaaacacg gtgtaccagt acgacgaaat
     1621 cgatcagcgg ctggtggacg aacgggttgc ccagttccgc gaccagaccc accgctttct
     1681 ggccggaaag ctgtcggagg acgattaccg cccgctgcgg ctgagaaacg gcctctacat
     1741 tcagcgctac gcgccgatgc tgcgcgtggc gattccttac ggtctgctgt cttcgcgcca
     1801 gttgcgcacg ctcgcgcaca tcgcccgcaa gtgggaccgg ggttacggcc acttcagcac
     1861 gcgccagaac atccagtaca actggccgaa actggaggat gttcccgata tcctggccga
     1921 gctggcgaag gtggaaatgc acgcgatcca gaccagcggc aattcctttc gcaacatcac
     1981 gaccgatcac tacgcgggcg ttgcgcgcga cgagatcgtg gattccttcg tctggtgcga
     2041 gctgctgcgc cagtggtcca cgtttcaccc cgaattcact tacctgccgc gcaagttcaa
     2101 gatcgcggtg aacggggcgg cggccgaccg cgcggccgtc tttctccacg acatcgggct
     2161 gcaggcgctg cgagatgacg cggacgaagt cggcttcaag gtcatcgtcg gcggcggcat
     2221 gggacgcact ccgatcgtcg ggcacgtggt ccgcgaattc ctgccctggc ggcacctgtt
     2281 tacctatgtc gaggcggtgc tccgggtcta taaccgccac ggccggcgcg acaacctgta
     2341 caaggcgcgc atcaagatcc tggtgaagga gcgcacgccg gataagttcc gggaggaagt
     2401 c
//
LOCUS       DBNGSG010000259         4081 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_760737, whole genome shotgun sequence.
ACCESSION   DBNGSG010000259 DBNGSG010000000
VERSION     DBNGSG010000259.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_760737"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            664..1431
                     /locus_tag="AC158Q_06730"
     CDS             664..1431
                     /locus_tag="AC158Q_06730"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HIR3339506.1"
                     /translation="MLESLSPLLLVWVLCVILVAGLLQGALGFGFPFVATPLIALVSD
                     MRTAIILVLLPTLATICVALAASGPLRATLARFWMMPLYMILGSAAGTWVFVSAPQAP
                     YTLVLALLTLFYLSLDRLGRTEWAVVKRNERAFAPLSGLAAGAFEGTANVAAPPLIIL
                     YLSLSLTPAMLVQALNICFLVGKTTQFTVLSFRGGVGVNEWLATLPLVAIGVAGSLIG
                     VRIRNRIDAHAFRVWVKRALFVIALGLLAQYAWSLTE"
     gene            1531..2610
                     /gene="ada"
                     /locus_tag="AC158Q_06735"
     CDS             1531..2610
                     /gene="ada"
                     /locus_tag="AC158Q_06735"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HIR3339507.1"
                     /translation="MNLSSNNEILAARTKKDPRWAAVVRRDPGADGKFFYSVRTTGVY
                     CRPSCGSRRARPENVRFFASGADAERAGFRPCKRCRPDQPTRDQQLAAKVAETCRFIE
                     AAAETPGLRELAARAGLSAYHFHRVFKAVTGVTPRAYAAAHRARRVRGELRRGGTVTE
                     AIYGAGYGSNGRFYETADAVLGMTPGAYRAGGANTEIRFAIGKCALGSILVAQSARGI
                     CAIFLGDDPGALARELQDRFPRARLIGGDRAYEKLVAKVVGFVEAPALGLDLPLDVRG
                     TAFQQRVWQALRRIPAGRTASYTEIAKRIGSPQSVRAVAGACAANALAVAIPCHRVVR
                     NDGGLSGYRWGVERKRALLKREAGT"
     gene            complement(2681..2998)
                     /gene="grxD"
                     /locus_tag="AC158Q_06740"
     CDS             complement(2681..2998)
                     /gene="grxD"
                     /locus_tag="AC158Q_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HIR3339508.1"
                     /translation="MSIQDVIKKQVVENRVVLYMKGSPDFPQCGFSANAVQILRACGA
                     NDIYTVNVLENPDIRQGIKEYANWPTIPQLYVNGEFVGGSDIMTEMFKNGELKKLLEK
                     TTA"
     gene            complement(3034..3867)
                     /gene="prmC"
                     /locus_tag="AC158Q_06745"
     CDS             complement(3034..3867)
                     /gene="prmC"
                     /locus_tag="AC158Q_06745"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008112568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIR3339509.1"
                     /translation="MTIAEALRIAGVDFADARVLLREILRADEAHLLAHPGQHLTEAQ
                     RAGYLAWVERRRSGEPVAYLTGSREFYGLALRVTPAVLIPRPETELLVEAALERIPAD
                     QVCRVLDLATGSGCVAVAIAKHRPRAQITATDVSSAALAIARENAARHGVSIEFFESD
                     WFAALAGRKFDLIVSNPPYVAEDDPHLDSGDLRFEPRSALAAGLAGLDSIEVIVEQAV
                     RHLASGGQLLFEHGYDQGPCSRTRLAAAGFLDIATRRDLAGIERVSGGGFDGAPGQKL
                     K"
     gene            complement(3893..>4081)
                     /locus_tag="AC158Q_06750"
     CDS             complement(3893..>4081)
                     /locus_tag="AC158Q_06750"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR3339510.1"
                     /translation="AVLAMYFALTTGPVQVVAPVVATYPLFTLALSALFLRHEQLTGR
                     LIGGVLLTVAGVVILLAR"
BASE COUNT          697 a         1355 c         1286 g          743 t
ORIGIN      
        1 catgggctct cctcaggtaa cggcgcagct gtcttcggcc ggaccggtca ttatacgctc
       61 cgatgaaagt gctgcaagcg ctttcattat tgaagataac tatatgaata ataatatatt
      121 aatggtatat catccgcggc catctcaggt tcggccgccc acctggccca acccgtcgac
      181 tacgggtcgg gcctgatacc cggagcaccg cctggccggc gttttttcaa cccgcaaagc
      241 gcacttcgat ccagcgcggg cttggcgttt accaaacttc gagccgatat atatccaggc
      301 gttcaccggc cgcacctcaa attcttgcct ctcctattcc gtttttcgcg acgctcgatt
      361 gctgcgaaaa acgatacact gcggcttcgc atgcctgctt gcctccgcgt tgcgccatga
      421 gtgcgtctgc cgcgccccat tccctccagt ccgatgccgc cgcgctcgag cgcgccttgc
      481 gcgcgcggcg gcggcatttc gcggtgcggg cagtgcgtcg aagtcgctgt ggtgatcgac
      541 gcctcgcgcc atctcaaccg tgcgcgtcgc gcgctcccta taaccgaggt cgcccgcctg
      601 tctctggcga aaaaagcatg agccccatca cccaacactc atcacccatc acgcctttcg
      661 aacgtgcttg agtcgctctc gccgctgctg ctcgtgtggg tgctttgcgt gatcctggtc
      721 gccggcctgc tccagggcgc gcttggcttc ggctttccgt tcgtggcgac gccgctgatc
      781 gcgctggtga gcgatatgcg caccgcgatc atcctggtgt tgctgccgac gctggcgacc
      841 atctgcgtcg cgctcgccgc cagcggtccg ctgcgcgcca cgctcgcgcg cttctggatg
      901 atgccgctct acatgatcct ggggtcggcc gccggcacct gggtattcgt gtccgcgccg
      961 caagcaccgt atacgctcgt gctggcgctg ctgacgctgt tctatctcag cctcgaccgc
     1021 ctgggccgca ccgagtgggc ggtcgtcaag cgcaacgagc gggcgttcgc gccattgtcc
     1081 ggcctcgccg ccggagcgtt cgaaggcacg gcaaacgtcg ccgcgccgcc gctcatcatc
     1141 ctctacctct cgctcagcct gacgcccgcc atgctggtgc aggcgttgaa catctgcttc
     1201 ctggtgggca agaccacgca gttcacggtg ctcagttttc gcggcggcgt cggcgtaaac
     1261 gaatggctcg cgacgctgcc gctggtcgcg atcggcgtgg ccggttcgtt gatcggcgtg
     1321 cgcatccgca accgcatcga tgcgcatgcg ttccgcgtct gggtaaagcg cgcgctattt
     1381 gtcatcgcgc tgggactgct ggcgcagtac gcctggtccc tgactgagta gcgccgggtg
     1441 aagcggaatt caacagcaag agccggagtg cggtcccctg gcgaggccga ttaagctatg
     1501 ccaaccttga acggattggc agacacgacc atgaacctca gctccaacaa cgaaatactc
     1561 gccgccagga cgaagaaaga ccctcgttgg gcggccgtcg tcaggcgcga ccccggcgcg
     1621 gacggcaaat tcttctactc ggtcaggacc accggcgtgt actgccgccc ttcctgcggg
     1681 tcgcgtcgcg cacggccgga gaatgtgcgg tttttcgcct cgggagccga tgccgagcgg
     1741 gcgggttttc gcccctgcaa acgctgccgg cccgatcagc ccacgcggga ccagcagctt
     1801 gcggcaaagg ttgcggaaac ctgtcgcttc atcgaggccg ctgctgaaac gccgggcctg
     1861 cgcgaactgg ccgccagggc gggactgagc gcttaccact ttcaccgcgt cttcaaggcc
     1921 gtgacggggg taacgcccag ggcttacgcg gcggctcacc gcgccaggcg ggttcgcgga
     1981 gaattgcgcc ggggcggcac ggtcacggag gcgatttacg gcgccggtta cggctccaac
     2041 ggacgcttct acgaaaccgc ggacgcggta ctcggaatga cgccgggcgc gtaccgcgcg
     2101 ggcggcgcga acacggagat ccggtttgcc atcggcaagt gcgcgctcgg ctccatactt
     2161 gtcgcgcaga gcgcgcgcgg catctgcgcc atcttcctcg gcgacgaccc gggagcactg
     2221 gcgcgcgaac tgcaggatcg cttcccgcgc gcccgcttga tcgggggcga cagggcatac
     2281 gagaagctgg tggccaaagt ggtgggattc gtggaggccc ccgcactggg cctcgacctg
     2341 ccgctcgatg tgcgcgggac tgcgtttcag caacgcgtct ggcaggcact gcgcaggatt
     2401 cccgccggca ggaccgcgag ctacacggaa atcgcgaagc ggatcggatc gccccagtcg
     2461 gtgagagcgg tggcgggcgc gtgcgcagcc aacgcgctgg cggttgcgat tccgtgccat
     2521 cgcgtcgtca ggaacgacgg agggctgtca ggctatcgct ggggcgtcga gcgcaagcgg
     2581 gcgctgctga aacgcgaagc cggtacgtaa tggcgagatc aatgccagaa aagaaaacgg
     2641 ccagcgatca actggccgtt ttccatgggc aagacgttgc tcaggcagtg gtcttttcca
     2701 gcagcttctt cagctcgccg ttcttgaaca tctcggtcat gatgtccgag ccaccgacga
     2761 attcgccgtt cacgtagagc tggggaatgg tcggccagtt ggcgtattcc ttgatgccct
     2821 ggcggatatc gggattctcc agcacgttga cggtgtagat atcgttcgcg ccgcaggcgc
     2881 gcaggatctg caccgcgttg gcggaaaacc cgcactgcgg aaaatcgggc gaacccttca
     2941 tgtaaagcac gacccggttt tcgactacct gtttctttat aacatcctga atactcatgg
     3001 tttattccta tttccgagta aaacactcaa acattatttt aacttctgcc ccggggctcc
     3061 gtcaaaccct cccccggaga cccgctcgat gccggccagg tcgcggcggg tggcgatatc
     3121 cagaaatccc gccgcagcga gccgcgtgcg cgagcagggt ccctggtcgt aaccatgctc
     3181 gaacaggagc tgaccgccgg acgccaggtg gcgcaccgcc tgctccacga tgacttcgat
     3241 gctgtcgagc cccgccagcc ccgccgcgag cgcgctgcgc ggctcgaagc gcaagtcgcc
     3301 tgagtcgagg tgcggatcgt cctcggcgac gtacgggggg ttcgatacga tgagatcgaa
     3361 tttccgtccc gccagcgccg cgaaccagtc gctttcgaag aattcgatac tgacgccgtg
     3421 ccgcgccgcg ttctcgcgcg cgattgcgag tgccgcggac gacacgtcgg tcgcggttat
     3481 ctgcgcccgg ggacggtgtt tggcgatcgc aaccgcaaca caaccgctgc cggtcgccag
     3541 atcgagcacc cggcacactt gatccgccgg aattcgctcg agcgcggcct cgaccagcag
     3601 ctcggtttcc ggccgcggaa tcaataccgc cggcgtcacc cgtaatgcga ggccgtagaa
     3661 ctcgcggctg cccgtgagat acgcgaccgg ctcgccgctg cgccggcgct ccacccacgc
     3721 aagatagccc gcgcgctggg cttcggtgag atgctgcccg gggtgtgcga gaagatgcgc
     3781 ctcgtccgcc cgcaggattt cccgcagcag cacgcgggca tcggcgaaat caacccccgc
     3841 gatgcgcagc gcctcggcta tcgtcatgga cgacctcaat ttggctgatc ggttaccggg
     3901 ccagcaggat aacaacgccc gcgacggtga gcagcacccc cccgatcagc cggccggtca
     3961 actgctcgtg ccgcaagaac aacgcgctca gggcgagcgt gaacagcgga taggtcgcaa
     4021 caaccggcgc aacgacctgg accgggccgg tggtgagcgc gaagtacatc gcgagcaccg
     4081 c
//
LOCUS       DBNGSG010000260         3751 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1269498, whole genome shotgun sequence.
ACCESSION   DBNGSG010000260 DBNGSG010000000
VERSION     DBNGSG010000260.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1269498"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..169
                     /locus_tag="AC158Q_06755"
     CDS             <1..169
                     /locus_tag="AC158Q_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR3339511.1"
                     /translation="TMIVVTHEMGFANKVAHRVIFMDQGRIVEDAPKAEFFGKPRSER
                     AVQFLSKILHH"
     gene            complement(538..1713)
                     /gene="argE"
                     /locus_tag="AC158Q_06760"
     CDS             complement(538..1713)
                     /gene="argE"
                     /locus_tag="AC158Q_06760"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013691296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HIR3339512.1"
                     /translation="MGSDTARPLSSVEMLRALVAFPTVSRDSNLALIHYVRDALAPCK
                     ADVRLTYDDGRRKANLFATLGPRGAGGIALSGHTDVVPVEGQAWDTDPFSMVEKDGRL
                     YGRGTSDMKGFIACVLALVPEFMERGLKAPLHLAFSYDEEVGCIGVGRLISDLRSAGI
                     RPRSCIVGEPTEMRPVIAHKGKKGYRCTVRGLAGHSAYAPHGVNAVEYAAEAVAFLKG
                     LARRHRDRGPYDRGFDIAHTTVHTGVIRGGTALNVIPHDCAFDFEFRHLPGDDPDRLL
                     HEFEDYLRGKLEPEMRAVHAAAGFDIEPLSAIPVLDTGPESEVVALAQELSGNSEFGK
                     VSFGTEGSQFQQAGIPTVICGPGSIAQAHKPNEYVTLDQIKRCEAFLRRLMDRVCKA"
     gene            1845..3398
                     /locus_tag="AC158Q_06765"
     CDS             1845..3398
                     /locus_tag="AC158Q_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3339513.1"
                     /translation="MKTLPAVVLLALSLSTSAADLSIALGGDVTSIDPHFHNLTPNNN
                     VGAHVFDSLVMKDASGRIKPGLAESWRAVDDLTWEFQLRKGVKFHDGTDFTAADVAFS
                     LDRVPNVPNSPSPFTTYTKQVTEKIVVDPYTLRLKSAAPYPQMPNDMSTVLIVSAKAA
                     AGATTDDFNSGKATIGTGPFKFVRWQKGDRIELARNDAYWGRKSPWDKVTFRIITSDP
                     TRVASLLAGEVRAIENVPTNDLARLGANKDLSLFRTVSVRLMYLHMDTARDKSPFAMD
                     KAGRPLDKNPLRDLRVRRAMSKAINRQALVERVMEGAAVATGQLMPEKFFGHTPALRT
                     EPYDPEGARKLLAEAGYPDGFGLTLHAPNNRYVNDEQIAQAIAQMLSRIGIQTRVDAM
                     PSSVYFSRGSKLEFSLMLVGWGAETMEASSPLKALLATFSKEKGMGAANRGRYSNTRL
                     DAALGQALATVDDARREKLLQQATEIGVGDLGIIPLYHQHNLWAARRGITYDARADER
                     TFAHEFRPQ"
     gene            3554..>3751
                     /locus_tag="AC158Q_06770"
     CDS             3554..>3751
                     /locus_tag="AC158Q_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3339514.1"
                     /translation="MSCLAAAQAAELRIGLSADVTTMDPHFVAAQPNLTAQQHVFEAL
                     IQTDARTRPVPGLASWSNPDSL"
BASE COUNT          728 a         1244 c         1140 g          639 t
ORIGIN      
        1 gaccatgatc gtcgtcaccc acgagatggg ctttgcgaac aaggtcgcgc accgcgtcat
       61 cttcatggat cagggccgca tcgtcgaaga tgcgcccaaa gcggaattct tcggcaagcc
      121 gcgctcggag cgggcggtgc agttcctgtc gaaaattctc caccactaaa tcgaaccgcc
      181 aagacgccaa ggcgcagaga aaacccagat ctataatgaa ccgccaaggc cgccaagaaa
      241 ctcaataaca atgagtgtca actaatttct ggcatctaat tcttgtcgac taatctaaga
      301 acacctaaca aaatgaaaat cggcgaccga ccgttcatgg ttcgacacgc tcaccacgaa
      361 cgggccgttc gccctgagct cgtcgaaggg caacgagccc ggtcattttg ttaggcgttc
      421 taagttaaat tgcttctttg cttctctcga cgtctcgctg gtctaattat tgttatctgt
      481 ttttcttggc ggccttggcg tcccttcgac tcgctcagga caggcttggc ggttaattca
      541 agctttgcaa acgcgatcca tcagccgccg caggaacgcc tcgcagcgct tgatctgatc
      601 gagcgtcacg tactcgttcg gcttgtgcgc ctgcgcgatc gagcccgggc cacagatgac
      661 ggtcggtatc cctgcctgct ggaactgtga accttcggtg ccgaacgaaa ccttgccgaa
      721 ttcgctgttg cccgacagct cctgggcgag cgccaccacc tcgctctccg gaccggtgtc
      781 cagcaccggt atcgccgaaa gcggctcgat gtcgaaaccc gccgcggcat ggaccgcgcg
      841 catttccggc tccagtttgc ccctgagata atcctcgaac tcgtgcagca gccggtcggg
      901 atcgtcgccc ggcaggtgcc ggaactcgaa atcgaacgcg cagtcatgcg gaatgacgtt
      961 cagcgccgtg ccgccgcgaa tcacgccggt atgcacggtg gtgtgagcga tgtcgaagcc
     1021 gcggtcatac ggcccccggt cgcggtgacg gcgcgcgagg cccttcagga aagcgaccgc
     1081 ctccgccgcg tactcgacgg cgttgacgcc gtgcggcgca taagcggagt gcccggcgag
     1141 cccgcgcacc gtgcagcggt atcctttctt gcccttgtgc gcgatcaccg ggcgcatttc
     1201 ggtcggctcg ccgacgatgc atgagcgcgg gcggatgccg gcactgcgca aatcggagat
     1261 gagccgcccc acgcctatgc agcccacctc ctcgtcgtag gaaaacgcga ggtgcagcgg
     1321 cgccttgagt cccctctcca tgaattcggg caccagcgcc agcacgcagg cgatgaagcc
     1381 cttcatatcg gaagtgccgc ggccatacag ccggccgtcc ttctcgacca tcgagaacgg
     1441 atcggtgtcc caggcctgcc cttcgaccgg cacgacgtcg gtgtgcccgg agagcgcgat
     1501 gccgcccgcc ccgcgcggac ccagcgtggc gaacaggttg gccttgcgcc tcccgtcgtc
     1561 gtaggtgagc cgcacgtccg ccttgcaggg cgcgagagcg tcacgcacat aatggatcag
     1621 cgcgaggttc gaatcgcggc tcaccgtcgg aaacgcgacg agcgcccgca acatctcgac
     1681 actggacaaa ggcctggctg tatccgatcc catgacgccc gcggcaggag gtggttcgtc
     1741 gagcataggg aggcggtacg ggagtgtcaa accgcaaagc gcgcgggcac aggcgcgccg
     1801 atttacggta agcttcagga agcccgagcg ctcaaggaag aaacatgaaa acgcttccag
     1861 ccgtagtact tctcgccctc tcgctctcga cctctgccgc cgatctcagc atcgccctcg
     1921 gcggagacgt gacctcgatc gacccgcact tccacaacct cacgccgaac aacaacgtcg
     1981 gcgcgcacgt gttcgattcg ctggtgatga aggatgcaag cggccgcatc aagcccggcc
     2041 tcgcggaatc gtggcgcgcg gtggacgatc tcacctggga attccagctg cgcaaaggtg
     2101 tcaaattcca cgacggcacg gattttaccg ccgccgacgt cgcgttctcg ctcgaccgcg
     2161 tcccgaacgt gccgaacagc ccttcgcctt tcaccacgta taccaagcag gtgaccgaga
     2221 agatcgtggt ggacccgtac acgctgcggc tgaagagcgc ggcgccctac ccgcaaatgc
     2281 cgaacgacat gagcaccgtg ctgatcgtct ccgcgaaggc ggcggcgggc gccacgacgg
     2341 acgatttcaa ttcgggcaaa gccacgatcg gcaccggccc gttcaagttc gtgcgctggc
     2401 agaaaggcga ccgcatcgag ctcgcccgca acgacgccta ctgggggcgg aaatccccgt
     2461 gggacaaggt cactttccgc atcatcactt ccgacccgac gcgcgtcgcg tcgctgctgg
     2521 cgggggaggt gcgcgccatc gagaacgtgc ccaccaacga cctggcgagg ctcggcgcca
     2581 acaaggacct gagccttttc cgcacggtgt cggtgcggct catgtacctc cacatggaca
     2641 cggcgcgcga caagtcgccg ttcgcgatgg acaaggcggg acggccgctg gacaagaatc
     2701 cgctccggga cctgcgcgtg cggcgtgcga tgtcgaaggc gatcaaccgc caggcgctgg
     2761 tcgagcgcgt catggagggc gccgccgtcg ccaccgggca gctcatgcca gagaaattct
     2821 tcggccacac gccggcgctc cgcaccgagc cgtacgatcc ggaaggcgcg cgcaagctgc
     2881 tcgccgaagc gggctacccc gacggcttcg ggctgacgct gcacgccccc aacaaccgat
     2941 acgtgaacga cgagcagatc gcacaggcca tcgcgcagat gctgtcgagg atcggcatcc
     3001 agacgcgggt ggacgccatg ccgtcctcgg tgtacttctc gcgcggctcg aagctggagt
     3061 tcagcctcat gctggtcggc tggggcgcgg aaaccatgga agcctcctcg cccctgaaag
     3121 ccctgctcgc gaccttcagc aaggaaaaag ggatgggagc ggcgaaccgc ggccggtatt
     3181 ccaataccag gcttgacgcg gcgctgggcc aggcccttgc gacggtggac gacgcccggc
     3241 gcgagaagct gctgcagcag gccaccgaga tcggcgtcgg cgatcttggc attatcccgc
     3301 tctaccacca gcacaacctg tgggcggcgc gcaggggcat cacttacgat gcgcgcgcgg
     3361 acgaacgcac cttcgcacac gagtttcgcc cgcagtaggc ttcaaaagcg tgaatggtga
     3421 gtggtgaatg gtgattggat tcttgaaaaa cgttttgctg cgccggccgc tggcccgggt
     3481 gaatgaggac cattcaccaa tcactaatca ccaatcacgc atttctgctg ccgcgagctg
     3541 gcttctgctt tatttgtcat gcctcgcggc agctcaagca gcggagctgc gcatcggcct
     3601 gtcggccgac gtcacgacga tggacccgca cttcgtcgcg gcgcaaccca acctgaccgc
     3661 gcagcagcac gtcttcgagg cgctcatcca gaccgacgcg cgcacccgcc cggtgcccgg
     3721 gcttgcctcc tggagcaatc cggattcgct c
//
LOCUS       DBNGSG010000261         1614 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3479177, whole genome shotgun sequence.
ACCESSION   DBNGSG010000261 DBNGSG010000000
VERSION     DBNGSG010000261.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3479177"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="AC158Q_06775"
     CDS             <1..914
                     /locus_tag="AC158Q_06775"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-linked N-acetylglucosamine transferase,
                     SPINDLY family protein"
                     /protein_id="HIR3339515.1"
                     /translation="AIHADGIDVLVDLTGYTQWGRPGILALRPAPVQVNFLGFPGTLG
                     ADFADYILADDFLVPPGESRHYTEQIAFLPRFQPNDHRRPRPGTASREEHGLPRDALV
                     LCCFNQTFKISPPLFDIWMRLLAAVPGAVLWLTRSNPRVEDNLRAEAARRGVDPRRLV
                     FAPWIQGDLARHYARVALADLFLDTAPFNAGATASDALWCGVPVLTRAGDSFVSRAAG
                     SLLTALALPELITSTPQQYERRALELARDRSRLAALRARLAHNRDTGKLFDTPSYVRN
                     LESAYRLMWERYRAGEPPGALRVSAGG"
     gene            1404..>1614
                     /locus_tag="AC158Q_06780"
     CDS             1404..>1614
                     /locus_tag="AC158Q_06780"
                     /inference="COORDINATES: protein motif:HMM:NF012573.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HIR3339516.1"
                     /translation="MAIIINPEGQTYTGGAGDDELIGTALDDTLIGLGGNDLLDGAEG
                     HDSIVGGDGHDELFGYGGEDTLIAGA"
BASE COUNT          253 a          573 c          534 g          254 t
ORIGIN      
        1 gcgcgatcca cgccgacggc atcgacgtcc tggtggacct gaccggctac acccagtggg
       61 gccggcccgg catcctcgcg ctgcggccgg cgccggtgca ggtgaacttc ctcggcttcc
      121 ccggcacgct cggcgcggat ttcgccgact acatccttgc cgacgatttc ctcgtgccgc
      181 cgggcgagtc ccgccactac acggagcaga tcgcctttct gccgcgcttc cagccgaacg
      241 accaccggcg cccgcgcccc ggcacggcgt cgcgcgaaga gcacgggctg ccgcgcgacg
      301 cgctggtgct ctgctgcttc aaccagacct tcaagatctc gccgccgctg ttcgacatct
      361 ggatgcggct gctggcggcg gtgcccggcg ccgtgctgtg gttgacgcgc tccaacccgc
      421 gcgtcgagga caacctgcgc gccgaggcgg cgcgccgcgg cgtggacccg cggcgcctgg
      481 tgttcgcgcc gtggatccag ggcgacctcg cgcgccacta cgcgcgcgtc gcgctcgccg
      541 atctcttcct cgacaccgcg ccgttcaacg ccggcgccac cgcgagcgac gcgctgtggt
      601 gcggcgtgcc ggtgctgacg cgcgccggcg acagcttcgt ctcgcgcgcc gccggcagcc
      661 tgctgaccgc gctcgcgctg ccggagctga tcacgtcaac gccgcagcag tacgagcgcc
      721 gcgcgctgga gctcgcgcgc gaccgcagcc gcctggcggc gctgcgcgcc cgcctggcgc
      781 ataaccgcga cactggaaag ctgtttgaca cgccgagcta cgtccgcaat ctggaatccg
      841 cctaccgcct catgtgggag cgctatcgcg cgggggagcc acccggcgcg ctgcgggttt
      901 ccgctggcgg ctgagcgtag cggcctgcgg caccgcacca ttccggttcc ggcggacagg
      961 ctcacgcccg tttcccggcc gggcgaaatg cgacgatact gaccggacgg gaaacagaat
     1021 agctttgtca aaacaatggc atgcaggttc ggcctacttc gggccgcgag ctgccctgct
     1081 ccgctgctga tctggaaccg gcacgggcac gttcaacgat caagcctgcc aatgcggtgc
     1141 ggcatgaggg gccgatgcgg gaaacggcgg cgcggtccgg cgtgacgaat tcgtgacgga
     1201 tttcacgctg ggaaatgagc ggcggcctcg gcccggggcg ccgccggttg cggctcgcaa
     1261 acacgctcga ataattcgct tgcaatgcac gcgccggcct gcctaaaatc aaccccaatc
     1321 ataaaagtgg aatcggtgcg ttcgctcgcg accggttccg ttgtgaaatt ttcgcaggta
     1381 aaaaatttta ggggactctc gccatggcta tcatcatcaa tcccgaaggg caaacctata
     1441 ccggcggcgc cggtgacgat gaactcatcg gcaccgcgct cgatgacacg ctgatcggtt
     1501 taggtggcaa cgacctcctc gacggggcgg aaggccacga ctcgatcgtc ggcggcgacg
     1561 gccacgacga gctcttcggc tacggtggcg aagacaccct catcgccggc gcgg
//
LOCUS       DBNGSG010000262         9568 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1441454, whole genome shotgun sequence.
ACCESSION   DBNGSG010000262 DBNGSG010000000
VERSION     DBNGSG010000262.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9568)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9568)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9568
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1441454"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /gene="atpA"
                     /locus_tag="AC158Q_06785"
     CDS             complement(<1..892)
                     /gene="atpA"
                     /locus_tag="AC158Q_06785"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012354280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIR3339517.1"
                     /translation="MQQLKASEISELIKNRIQNLALSADVRTQGTVVSVTDGICRVHG
                     LSDVMQGEMLEFPKNTFGLALNLERDSVGAVILGEYEHITEGDTVKCTGKILSVPIGP
                     ELLGRVVNSLGAPIDGKGPINAKLTDVIEKVAPGVIARQSVSQPVQTGLKAIDAMVPV
                     GRGQRELIIGDRQTGKTAVAVDTIINQKGKDLFCVYVAIGQKASTIANVVRKLEEHGA
                     MAYTIVVAAPASESAAMQFIAPYSGCTMGEYFRDRGQDALIIYDDLTKQAWAYRQVSL
                     LLRRPPGREAYPGDVFYLHSR"
     gene            complement(912..1448)
                     /locus_tag="AC158Q_06790"
     CDS             complement(912..1448)
                     /locus_tag="AC158Q_06790"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014895498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HIR3339518.1"
                     /translation="MAEAVTIARPYAEAAFAFADAGGTLAKWAEALDTMAQLVAHPEM
                     HAAIGNPRLTDEQLYGLFAVLCGDGLTAEMQNFVRVLIENGRLALAPEIRKHFLELKN
                     EREGVVDAVVESALPLDAAQTAALTADLERRFKRKVKAQVTVNKELIGGVRVQVGDEV
                     IDSSVRGQLAAMAAALRN"
     gene            complement(1452..1925)
                     /locus_tag="AC158Q_06795"
     CDS             complement(1452..1925)
                     /locus_tag="AC158Q_06795"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HIR3339519.1"
                     /translation="MSFNLTLIAQMLAFAAFILFTVKVVWPHLLRAIETRQKTIADGL
                     AAAERGRQDLESAAKRTAEMMGEARQQVQDTITQADKRGAQIIDEAKGAAKAEGDRLV
                     SAAKAEIEQEVARAKEALREQVAALAIAGAEKILRREVDAKAHADLLAQLKQELR"
     gene            complement(1968..2240)
                     /gene="atpE"
                     /locus_tag="AC158Q_06800"
     CDS             complement(1968..2240)
                     /gene="atpE"
                     /locus_tag="AC158Q_06800"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013204713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HIR3339520.1"
                     /translation="MEKLQLLAMVQAYTGIGIGLMVGLGAAGACIGVGIMCSKFLEAA
                     ARQPELTNSLQGKVFLLLGLIDASFIIGLGIAMFFAFASPLATPFR"
     gene            complement(2284..3093)
                     /gene="atpB"
                     /locus_tag="AC158Q_06805"
     CDS             complement(2284..3093)
                     /gene="atpB"
                     /locus_tag="AC158Q_06805"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIR3339521.1"
                     /translation="MAAGKELNPSDYIQHHLTFYRASVGEGEFWTLNVDTVVTSVLLG
                     VIGLGFIWWVVRGATPGVPGKRQAFVELLIEFVDNQVKGIFHHGDRNRFIAPAALTVF
                     VWVTLMNAMDFLPVDWVAGALGLVGLHEWRPVPTADINATFALSISVLLMSIYFAIAA
                     KGLKGWAHEQFCAPFGSHPLLWPANLLFNLVEYVSKTLSHSLRLFGNIYAGEILFFLL
                     WLWAATGLVGTFFGFLFGLGWAIFHILIVLLQAYIFMMLTIVYISMAHEHH"
     gene            complement(3100..3468)
                     /locus_tag="AC158Q_06810"
     CDS             complement(3100..3468)
                     /locus_tag="AC158Q_06810"
                     /inference="COORDINATES: protein motif:HMM:NF015834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HIR3339522.1"
                     /translation="MFHDLGKPIRTVLRWQLLATAALTLVAGAWAGAHSALSAGLGGS
                     VSIFSGLASAVVASSGKTGSAGGVVTAALTAEAVKIGLIVLLLWLVLANYDGVVVPAF
                     IGSFLVTVLIFAMAFFVREH"
     gene            complement(3569..4417)
                     /locus_tag="AC158Q_06815"
     CDS             complement(3569..4417)
                     /locus_tag="AC158Q_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR3339523.1"
                     /translation="MAKIKGLGRGLDALLGSAEEPAAGGDTLTTLAVEDLQPGRYQPR
                     ARMDPETLAELAESIKSQGVMQPILARPLGAGRYEIVAGERRWRAARMAGLTQVPVLV
                     REVPDRHALAIALIENIQREDLNPLEEATGVKRLIEEFGMTHAEAAGALGRSRAAITN
                     ALRLLELAPPVQELMREGKLDMGHARALLALPALKQIELAREAVERQLSVRQVEQMVA
                     GLGKRAAAGAHPRPDRDVARLEEEWSQRLGTTVKIQTGMKPGSGRLVIQYASLDQLDT
                     LLRKLN"
     gene            complement(4429..5196)
                     /locus_tag="AC158Q_06820"
     CDS             complement(4429..5196)
                     /locus_tag="AC158Q_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR3339524.1"
                     /translation="MPKILAITNQKGGVGKTTTGVNLAASLAAAQRRVLLVDLDPQGN
                     ATMGSGVDKRVLAATVYHVLLGERTIQDVRVRSENGGYDLAPANRELAGAEIELVEME
                     HRETRLKEALAKVASEYDFILIDCPPALNLLTVNGLTAAQAVMIPMQCEYYALEGLSD
                     LVQTIKKVRGHLNPTLEIEGLLRTMYDPRNMLAQQVSEQLIEHFGDKVYRTVIPRNVR
                     LAEAPSHGLPALKFDQASKGAQAYLALAGEMLNRASG"
     gene            complement(5199..5822)
                     /gene="rsmG"
                     /locus_tag="AC158Q_06825"
     CDS             complement(5199..5822)
                     /gene="rsmG"
                     /locus_tag="AC158Q_06825"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HIR3339525.1"
                     /translation="MNLAAQLAQGVAQLDLTLPSQAQPRLLEYLALLQKWNRIYNLTA
                     VREASRMVSHHLLDCLAIVPHVTVDTVLDVGSGAGLPGIPLALARPRTHVTLLESSHK
                     KAAFLQQAKFELKLDNVAVVCERVEAWRPGRSFELVISRAFSDLAEFVAAAGRHVAPG
                     GRLAAMKGVYPHEEITRLAPGWRLHEAVPLTVPGLHAQRHLLQLGPE"
     gene            complement(5824..7761)
                     /gene="mnmG"
                     /locus_tag="AC158Q_06830"
     CDS             complement(5824..7761)
                     /gene="mnmG"
                     /locus_tag="AC158Q_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010811274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HIR3339526.1"
                     /translation="MQHIDKYDVIVVGGGHAGTEAALAAARMGCETLLLTHSIETLGQ
                     MSCNPSIGGIGKGHLVRELDALDGAMAAATDEAGIQFRILNSRKGPAVRATRAQADRV
                     LYRKAIRGRLENQPRLRIFQQAVDDLLLEGDHVTGVVTQIGLRFSARAVVLTTGTFLA
                     GLIHVGLKNYQAGRAGDPPALTLAQRLRELNLPVGRLKTGTPPRIDGRSIDFSVMTEQ
                     PGDEPTPVFSFLGRREQHPPQVPCWITHTNPRTHEIIRAALDRSPLYTGVIEGVGPRY
                     CPSIEDKITRFAGKTSHQIFLEPEGLTVREFYPNGISTSLPYDAQQELVRSIRGLECA
                     HITRPGYAIEYDYFDPRGLKRSLETKAIAGLYFAGQINGTTGYEEAAVQGMLAGINAA
                     LCVKERAPWCPLRNEAYLGVLVDDLVTRGVSEPYRMFTSRAEYRLSLREDNADLRLTE
                     AGYRLGVVSEARWEAFSRKREAIAREQERLKSTWINPRLTTPDAIVSALGQSLEREHS
                     LAELLRRPDIGYEQLMTLPGAGPGVVDPKVAEQVEIQAKYQGYIERQREEIARSEHYE
                     NLRLPEVLDYRDVRGLSVEAQQKLNQHRPETLGQASRISGITPAAISVLLVHMKRKLA
                     STYFDKSTGPGIAGRAAKKTA"
     gene            complement(7953..9293)
                     /gene="mnmE"
                     /locus_tag="AC158Q_06835"
     CDS             complement(7953..9293)
                     /gene="mnmE"
                     /locus_tag="AC158Q_06835"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006763048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HIR3339527.1"
                     /translation="MSENDTIAAIATAPGLSGIGVVRLSGPWLGPIVAGLVGKPLPPR
                     VAVLSGFRDAHGETIDQGLALFFPAPHSYTGEDVLELQGHGGPVVLQQLLKRCLELGA
                     RTAMPGEFTQRAYLNDKLDLAQAESVADLICAATAEAARGALRSLQGVFSLKIEELVR
                     GLIELRTRVEATLDFPDEEVDFLKQTDADSRLAMLRIQLQEVLSASQQGSLLRDGVQV
                     VLAGQPNVGKSSLLNRLAGEELAIVTEIPGTTRDVIRQSISVEGVPVHIIDTAGLREA
                     REPVERLGIERTWAAIEKADLLLLLMDATQGESEADQEIRKRLPSDLPCIKVMNKIDL
                     LAGRPAQTGQNDLNTVWLSAKTGMGIDRLRKSLLGMAGWQGSGEGLFMARERHVHALG
                     RARAHLESAIRQGRHPELFAEELRLAQESLGTITGAFTSDDLLGEIFSRFCIGK"
     gene            complement(9340..>9568)
                     /locus_tag="AC158Q_06840"
     CDS             complement(9340..>9568)
                     /locus_tag="AC158Q_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HIR3339528.1"
                     /translation="LMAATMWIQTKLNPEPPDPVQARVMKIMPIVFSVFFFFFPAGLV
                     LYWLVNNVLSIAQQWKITRTLEHEAAAAKKH"
BASE COUNT         1679 a         3150 c         2908 g         1831 t
ORIGIN      
        1 gccgcgaatg gagatagaac acgtcgcccg gataggcctc gcgccccggg gggcggcgca
       61 gcagcagcga tacctggcga taggcccagg cctgcttggt caggtcgtcg taaatgatta
      121 gcgcgtcctg gccgcggtcg cggaagtact cgcccatggt gcagccagaa tacggtgcga
      181 tgaactgcat cgccgcggac tcggatgcgg gggccgcgac cacgatggtg taggccatcg
      241 cgccgtgttc ctcgagcttt ctcaccacgt tggcgatggt ggatgccttc tgcccgatcg
      301 cgacgtaaac gcagaagaga tccttgccct tctggttaat gatcgtatcc accgccaccg
      361 cggttttgcc ggtctggcgg tcgccgatga tcagctcgcg ctgccctcgg cctaccggca
      421 ccatcgcgtc gatcgccttc agccccgttt gcaccggctg cgacaccgat tgccgcgcga
      481 tcacgcccgg cgccactttt tcgatgacgt cggtcagctt ggcgttgatc gggcccttgc
      541 cgtcgatcgg cgcgcccagc gaattgacca cgcggccgag caactcgggg ccgatcggca
      601 ccgagaggat cttgccggtg catttgaccg tatcgccttc ggtgatgtgc tcgtattcac
      661 ccagaatcac cgcgccgacc gaatcgcgct cgaggttcaa cgccagcccg aaggtattct
      721 tcgggaattc cagcatctct ccctgcatca cgtcggagag cccgtgcacg cggcagatgc
      781 cgtcggtcac cgacaccacc gtgccctggg tgcggacgtc cgccgagagc gcaagattct
      841 ggatgcggtt cttgatcagc tcgctgatct ctgaggcttt gagttgttgc atggttacct
      901 tcctcggatt gctagtttct caatgcggcg gccatcgctg cgagctggcc gcgcaccgag
      961 ctgtctatca cttcgtcgcc gacctgcacc cgcacgccgc cgatcaattc cttgttcacc
     1021 gtcacctgcg ccttgacctt gcgcttgaag cggcgctcga ggtccgcggt gagcgccgcg
     1081 gtctgcgcgg catccagcgg aagggcgctc tccaccacgg catccaccac gccttcgcgc
     1141 tcgttcttca actccaggaa gtgcttgcgt atttcgggcg cgagcgcgag ccgcccgttt
     1201 tcgatcaaca cgcgcacgaa attctgcatc tccgcggtca acccgtcgcc gcacaacacg
     1261 gcaaaaaggc catagagttg ctcgtccgtc agcctgggat tgccgattgc cgcatgcatt
     1321 tccgggtgcg cgacgagctg cgccatcgtg tcgagcgcct ctgcccactt ggcgagcgtg
     1381 ccgcccgcgt cggcaaatgc gaacgccgct tccgcgtacg ggcgcgcgat ggtgacagct
     1441 tcggccatgg tttagcgcaa ctcctgcttc aactgcgcga gcagatcggc gtgcgccttg
     1501 gcgtccactt cccgccgcag gattttctcc gcgccggcaa tcgcaagggc tgccacctgt
     1561 tcgcgcagcg cttccttggc tcgggcgacc tcctgctcga tctcggcctt ggcggcggaa
     1621 accagccggt cgccttcggc cttggcggcg cctttggcct cgtcgatgat ctgcgccccg
     1681 cgtttgtcgg cctgggtaat ggtgtcctgc acctgctgcc tggcctcgcc catcatttcg
     1741 gccgtgcgct tggcggcgct ctccaggtcc tgcctgccgc gttccgccgc ggccagtccg
     1801 tcggcaatcg ttttctgccg cgtttcaatg gcgcgcagca ggtggggcca gaccaccttc
     1861 acggtgaaca ggatgaacgc cgcgaaggcg agcatctgtg caatcaacgt cagattgaag
     1921 ctcatacgtt ttccccggaa attactgcgc gcgccgcgcg cggcgttcta gcgaaacggt
     1981 gtcgccagcg ggctggcaaa agcgaagaac atcgcgatgc cgagaccgat gatgaacgag
     2041 gcatcgatca ggcccagcag caggaacact ttgccctgca gcgagttggt gagctcgggc
     2101 tggcgcgcgg ccgcctccag gaatttgctg cacatgatgc ccacgccaat gcatgcgccc
     2161 gcagcgccga ggcccaccat caggccgatg ccgatgccgg tgtaggcctg gaccatagcc
     2221 aacagctgaa gtttttccat tgtgatttcc tttcctgggc gaaaagccgg ttgttatggg
     2281 aaattagtgg tgctcgtgcg ccatcgaaat gtagacgatg gtcagcatca tgaagatata
     2341 ggcctgcagc agcacgatca gtatgtggaa gatcgcccat cccaggccga acaggaaccc
     2401 gaagaaggtg ccgaccagcc cggtcgccgc ccacagccac agcaggaaga acaggatctc
     2461 gccggcgtag atattgccga agagccgcag cgagtgtgac agcgttttcg agacgtattc
     2521 gaccaggttg aacagcaggt tggccggcca caatagcgga tgcgacccga acggcgcgca
     2581 gaactgctcg tgcgcccagc ccttgaggcc tttcgcggcg atcgcgaaat agatcgacat
     2641 cagcagcacc gagatcgaca gcgcgaaggt ggcattgatg tcggcagtcg gcaccgggcg
     2701 ccattcgtgc aggccgacga gtcccagcgc gccggcgacc cagtcgaccg gcaggaaatc
     2761 catggcgttc atcagcgtca cccacacgaa caccgtcaag gcggcgggcg cgatgaaccg
     2821 gttgcggtcg ccgtgatgaa aaatgccttt cacctgattg tcgacgaact cgatcaacag
     2881 ctcgacgaat gcctgccgct tgccgggcac ccctggagtc gccccgcgca ccacccacca
     2941 gatgaacccc aggccgatga cgccgagcag cacggacgtg accacggtgt cgacgttgag
     3001 cgtccagaac tcgccctcgc ccaccgacgc gcggtagaac gtcaggtggt gctggatgta
     3061 gtcagacggg ttgagttctt tgccggcagc catcgttgtc tagtgttcgc gcacaaagaa
     3121 cgccatggca aaaatcagga cggtcaccag gaacgacccg atgaaggccg gcaccacgac
     3181 accgtcgtag ttcgccaaca cgagccacag gagcagcacg atcaggccga ttttaacggc
     3241 ttcggccgtg agcgctgccg tgactacccc ccctgcggac cccgtcttgc ctgaagaggc
     3301 cacgaccgcc gacgcgagtc cggagaagat gctgaccgag ccgccgagac ccgcggacag
     3361 agcactatgg gcgccagccc aagccccggc aacaagcgtc agtgcggcgg tggccagcaa
     3421 ttgccaccgg agaacggtgc gtatcggctt gcccaggtcg tgaaacatgg cgttgatatt
     3481 ctccgcccgg gaaagccacg gcagtatagc cttgcggcgc gataggggtc aatttgtcgc
     3541 ggcgcagcaa acgcgtggtt gcgccgggtc agttgagctt gcgcagcaga gtgtccagtt
     3601 gatcgaggct cgcgtactgg atcaccagcc tgccgctgcc gggtttcatt ccggtttgta
     3661 ttttcaccgt ggttccgagc cgctggctcc attcttcctc gagccgcgcc acatcgcgat
     3721 cgggtctcgg gtgcgcgcct gcagccgccc gcttgcccag gcccgcgacc atttgctcga
     3781 cctggcgcac cgacagctgt cgttccaccg cttcgcgcgc gagttcgatc tgcttcagcg
     3841 ccggcagcgc cagcaaggcc cgcgcgtgtc ccatgtccag cttgccttcg cgcatcagtt
     3901 cctgcaccgg cggcgcgagt tccagcagcc gcagcgcatt ggtgatggcg gcgcgcgagc
     3961 ggccgagcgc gcccgcggct tcggcatgcg tcatgccgaa ttcctcgatc aggcgcttga
     4021 cgccggtggc ttcctcgagc gggttcaggt cctcgcgctg gatattctcg atcaacgcga
     4081 tggcaagcgc gtgccggtcg ggcacttcgc gcaccagcac cggcacctgc gtgagccccg
     4141 ccatgcgggc ggcccgccag cgccgttcac cggcgacaat ttcgtagcgt ccggctccca
     4201 gcggtcgcgc gaggatcggt tgcatcacgc cctgggattt gatcgattcg gccagttcgg
     4261 cgagcgtctc cgggtccatg cgcgcgcgcg gctggtaacg ccccggctgc aagtcctcca
     4321 ccgccagcgt agtgagtgtg tccccccccg ccgcgggctc ctctgccgag cccagcaggg
     4381 catccagccc ccgtcccagt cccttgatct tggccatcga ttcgctcctc atccgctagc
     4441 gcggttcagc atttcacctg caagcgcgag gtacgcctgc gcgcccttgg aagcctggtc
     4501 gaacttgagc gcgggcaggc catggctggg cgcttccgcc agccgcacgt tgcgcgggat
     4561 cacggtgcga taaaccttgt cgccaaaatg ctcgatcaat tgctccgaga cttgctgcgc
     4621 gagcatgttg cgcgggtcgt acatggtgcg caacagcccc tcgatttcca gcgtcggatt
     4681 gaggtgtccg cgcaccttct tgatggtctg cacaagatca gagaggcctt ccagcgcgta
     4741 gtactcgcac tgcatcggga tcatcaccgc ctgcgctgcg gtcagcccgt tgaccgtgag
     4801 caggttcagc gccggcggac aatcgatcag aatgaagtcg tactccgaag caaccttggc
     4861 gagcgcttct ttcagccgcg tctcgcggtg ctccatttcc accagttcga tttccgcacc
     4921 ggcaagttcg cggttggccg gcgccaggtc gtagccgccg ttttccgagc gtacccgcac
     4981 gtcctggatg gtgcgctcgc cgagcagcac gtgatagacc gtcgcggcca ggacgcgctt
     5041 gtccacgccg ctgcccatgg tggcgttacc ctgcggatcg aggtcgacca gcagcacccg
     5101 ccgctgcgct gcggcaagac tcgcggcaag attgaccccg gtggtggtct tgccgacgcc
     5161 gcccttctga ttggtgatgg cgagtatctt cggcatgttc attccgggcc cagctgcaac
     5221 agatggcgct gggcatgcaa tccgggtacg gtcagcggga ccgcctcgtg caagcgccat
     5281 cccggcgcca gccgggtgat ttcctcgtgc gggtagacgc ccttcatcgc cgcaagccgc
     5341 ccgcccgggg caacgtgccg tcccgccgca gcgacgaatt cggccagatc cgagaaggcg
     5401 cgggagatca ccaactcaaa cgagcgcccc ggccgccagg cctccacccg ctcgcacacc
     5461 accgccacgt tatcgagttt cagctcgaat ttcgcctgct ggagaaacgc ggccttcttg
     5521 tggctcgatt cgagcagcgt cacgtgcgtg cgcggtcgcg cgagagcgag cggaatgccg
     5581 ggcaatcccg cgccgctgcc aacgtcgagc accgtatcca ccgtcacgtg cggcacgatt
     5641 gcgaggcaat cgagcaagtg atggcttacc atcctggagg cttcgcgcac ggcggtcagg
     5701 ttgtaaatcc ggttccattt ctgcagcagc gccagatact ccagcaagcg cggctgcgcc
     5761 tgcgacggca acgtcaggtc gagctgggca acgccctgcg cgagctgcgc tgcgaggttc
     5821 atgtcacgct gtcttctttg cggcgcgtcc cgcgatcccg gggccggtcg acttatcgaa
     5881 gtaagtgctt gctaacttgc gcttcatgtg caccagcagc actgaaatcg cggctggcgt
     5941 gattccggag atacgcgaag cctggccgag cgtttcgggg cggtgctgat tgagtttttg
     6001 ctgtgcctcg accgaaagcc cgcgcacatc acggtaatcc aggacttccg gcagccgcag
     6061 gttctcgtaa tgctcggagc gcgcgatttc ctcgcgctgg cgttcgatgt agccctggta
     6121 cttcgcttgg atttccacct gttcggccac cttcggatcg acaacgccag gccccgcgcc
     6181 gggcagtgtc atcaactgct catagcctat gtccgggcgc cgcagcaact ctgctagtga
     6241 atgctcgcgc tcgagtgatt gccctagggc tgagacaatt gcatccggcg ttgtcaggcg
     6301 cggattgatc caggtgctct taagccgctc ctgttcccgg gctatcgctt cccgcttgcg
     6361 cgaaaacgcc tcccaccgcg cctcgctcac cacgcccagt cggtaacccg cttcggtcaa
     6421 acgcaggtcg gcgttgtcct cacgcaggga caaccggtat tccgcgcggc tcgtgaacat
     6481 gcggtacggc tcggatacac cgcgcgtgac gagatcgtcc accagcacac ccaggtaggc
     6541 ttcgttacgc agcgggcacc acggcgcgcg ttcctttacg cacagcgctg cattgatgcc
     6601 ggcgagcatg ccctgcaccg cggcttcttc gtaccccgtg gtgccgttga tctgcccggc
     6661 gaaatacagc ccggctatgg ccttggtctc gagtgatcgc ttcagcccgc gcggatcgaa
     6721 gtaatcgtac tcgatggcat agccgggtcg cgtgatgtgc gcgcactcga ggccgcggat
     6781 cgaacgcacc agttcctgct gcgcgtcata cggcaggctg gtggagatgc cgttgggata
     6841 gaactctcgg acagtgagtc cttctggctc gagaaaaatc tggtgcgacg tcttcccggc
     6901 aaagcgcgtt atcttgtcct cgatcgacgg acaatagcgc gggccgacgc cttcgatcac
     6961 gccggtgtag agcggcgagc gatcgagcgc ggcgcggatg atctcgtgcg tgcgcgggtt
     7021 ggtgtgcgtg atccagcacg gcacttgcgg cggatgctgt tcgcggcgtc ccagaaaaga
     7081 aaacaccggc gtgggttcgt cgccgggctg ctccgtcatt acggagaaat cgatgctgcg
     7141 cccgtcgatg cgcggcggcg tgccggtttt caagcggccg acgggaagat tcagctcgcg
     7201 caaccgctgc gcaagcgtca acgccggcgg gtcgcctgca cgccccgcct gataattctt
     7261 caggccgacg tgaatcagcc ctgcgagaaa agtaccggtc gtgagcacga ccgcgcgagc
     7321 ggagaacctg agcccgatct gcgtcacgac cccggtgacg tgatcacctt cgagcagcaa
     7381 gtcatcgacg gcttgctgaa aaatacgcag ccgcggctgg ttctcgagcc ggccgcgtat
     7441 cgccttcctg tagagcacgc ggtcggcctg cgcgcgcgtt gcacgcaccg ccgggccctt
     7501 gcgtgaatta agaatgcgaa actggatgcc ggcctcgtcg gttgcggcgg ccattgcgcc
     7561 gtccagtgcg tccagctccc gcaccaggtg gcctttgccg atgcccccaa tcgagggatt
     7621 gcaggacatc tggcccaggg tttcgatgct gtgggtcagc agcagcgttt cacaacccat
     7681 gcgcgcggca gcaagggccg cttcggtacc ggcatggcca ccaccaacca caataacatc
     7741 atatttatca atatgttgca tcaatatttc caaggcaatg ctgtgagcac gcaatgatac
     7801 cgtaaattca gcagcggcag aacctgcaag caggcatgtt ccacgtgaaa cctttgatgc
     7861 tgcaatgcag catgacaatg tatatatgtt tgttccacgt gaaacattcc cagggtgtcg
     7921 ataggagcga aatgcccctc agacccaggg tattactttc ctatacaaaa ccggctgaat
     7981 atttccccca gcagatcgtc ggaagtgaat gcccccgtaa tggtccccag ggactcctga
     8041 gccagtctca gctcttctgc gaacagctcc gggtggcgtc cttgccggat cgcgctttcc
     8101 aagtgtgccc gcgcccgtcc caaggcgtgc acatggcgct cgcgcgccat gaacaaccct
     8161 tccccactgc cttgccagcc cgccatcccc aatagtgatt tacggagtct gtcgatgccc
     8221 atgcctgtct tggctgacag ccagaccgta ttcagatcgt tctgtcccgt ttgcgcaggc
     8281 cgccctgcca acaggtcgat cttgttcatg accttgatgc agggcaggtc cgatgggagt
     8341 ctttttcgga tttcctggtc cgcttcactc tcaccctgtg tcgcgtccat cagcaacagc
     8401 agcaaatcgg ctttctcgat ggctgcccag gtgcgctcga tgcctaggcg ctcgacgggt
     8461 tcacgagcct ctcgcaagcc cgccgtatcg atgatatgaa ccgggacacc ttccacgctg
     8521 atgctctgcc ggatcacgtc gcgggtggtt ccgggaattt ctgtgacgat ggccagttcc
     8581 tcgcccgcaa gacggttgag caggctcgac ttgcccacat tgggttggcc tgccaacacg
     8641 acttgcactc catcccgcag caggctgcct tgttgtgaag ccgacagcac ttcctgcaac
     8701 tggatgcgca gcatcgcgag cctgctatcc gcatccgtct gtttcaggaa atcgacttcc
     8761 tcatcgggaa aatccagcgt ggcttcgacc cgagtccgca attcgatcag tccgcgcacc
     8821 agctcctcga tcttgagcga gaacacgccc tggagtgagc gcaaagcacc gcgggctgct
     8881 tctgccgtgg ctgcgcagat caagtccgct acgctctcgg cttgcgcaag gtcgagcttg
     8941 tcgttgagat aggcccgctg agtgaactcg cccggcattg ccgtacgcgc acccagttcg
     9001 aggcagcgct tgagcaactg ctgcagcacg actggcccgc catgcccctg caactcgagt
     9061 acgtcttcgc cggtgtagga gtgtggcgcc gggaaaaaaa gcgcgaggcc ctggtcgatt
     9121 gtctcgccat gcgcatcgcg gaatccggac agcacggcca cgcgtggagg cagcggcttg
     9181 ccgacgaggc ccgctacgat gggacccagc cacggaccgc tgagtcgcac cacgccgata
     9241 ccgctcaacc cgggcgcggt cgcaatggcg gcgatggtgt cgttctcgct catcaccctg
     9301 cagatgccct ggcgcgggcc ctcgacggta ctgctgatgt cagtgtttct tggccgccgc
     9361 tgcctcgtgt tccagcgtgc gcgtaatttt ccactgctgc gcgatcgaga gcacgttgtt
     9421 caccagccag tagagcacca gccccgccgg gaaaaagaag aagaagacgc tgaacacgat
     9481 cggcatgatc ttcatgaccc gcgcctgcac cggatccggc ggctcgggat tgagtttggt
     9541 ctggatccac atggtcgcgg ccatcagg
//
LOCUS       DBNGSG010000263         3291 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3480158, whole genome shotgun sequence.
ACCESSION   DBNGSG010000263 DBNGSG010000000
VERSION     DBNGSG010000263.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3480158"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="AC158Q_06845"
     CDS             complement(<1..498)
                     /locus_tag="AC158Q_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HIR3339529.1"
                     /translation="MKHSLQLRLSQHLTLTPQLQQSIRLLQLSTLELNQELERILQDN
                     PLLERDDEAREYPVTAVENVPHDVAASTPATEAPPADAEPGDRENADFSALDDAPASG
                     YRDDAEDGEYQQVAADAPTLRAHLLAQLSLTNLPERDRRLAALLIDSLDDDGYLTQGL
                     EELAAL"
     gene            complement(657..1451)
                     /gene="lptB"
                     /locus_tag="AC158Q_06850"
     CDS             complement(657..1451)
                     /gene="lptB"
                     /locus_tag="AC158Q_06850"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HIR3339530.1"
                     /translation="MPITLNKLADDPGQAAAAMPATARNYELRAQRLSKRYRSRVVVK
                     DVSLEVRSGEVIGLLGPNGAGKTTCFYMMVGLVPMDGGELHLDDIRLTHMPMHRRARL
                     GLSYLPQEASIFRRLTVAENVQAILELYNDDPEVISNKLDSLLLDLHVSHLHASPAIS
                     LSGGERRRVEIARALATEPRFILLDEPFAGVDPIAVLDIQKIIGFLKARGIGVLITDH
                     NVRETLGICDRAYIINDGGVLAYGKPDEIVYNESVRKVYLGEHFRL"
     gene            complement(1481..2137)
                     /gene="lptA"
                     /locus_tag="AC158Q_06855"
     CDS             complement(1481..2137)
                     /gene="lptA"
                     /locus_tag="AC158Q_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HIR3339531.1"
                     /translation="MIRAKLRSGDAASARTGRPGRALAALCVLAALALPASAEKADRD
                     KPVNLEADRVTIDDAKQIATFEGNVVLSQGTLQIRGDRMEVRQDKAGFKQGTTWGSPA
                     SFRQKREGYDEYIEGWAERIEYDGRIETLQMFDRAQLKRGQDEVRGNYISYDARTEFF
                     QVTGGTRNATAPGAPEGRVRAVIQPKSKENPPAAPPVSLKPAAAIATPREEAGARKTP
                     "
     gene            complement(2109..2696)
                     /gene="lptC"
                     /locus_tag="AC158Q_06860"
     CDS             complement(2109..2696)
                     /gene="lptC"
                     /locus_tag="AC158Q_06860"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HIR3339532.1"
                     /translation="MRKNESLTTAAPLLLVGMLAALTFWLDLVSQPPERGAGPSRNDP
                     DYIVDGLSAVSMSEAGAASYTLSAVKMMHYPEGDTTLLTAPKFVNYGKAKAPVTITAS
                     EAVVSANGQHVYFQDNVRLTRAAHGPNSELVVRTSFLHVIPDENLAKTDRAVTLSDAA
                     TTVTADGLELNNETRVIKLLSSVRGTYDPGKAPQR"
     gene            complement(2683..3210)
                     /locus_tag="AC158Q_06865"
     CDS             complement(2683..3210)
                     /locus_tag="AC158Q_06865"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KdsC family phosphatase"
                     /protein_id="HIR3339533.1"
                     /translation="MQEIYERARNIRVAAFDVDGVLTDGALYYADSGEELKRFSVHDG
                     HGMKMLKDSGIQLAIITSRRSRCVEERARNLGIELLYQGATDKLAAFRELLGRCGVDA
                     AACAYVGDDLVDLPVMKRCGLAVTVPGAPAPVRRQAHFVTRRPGGGGAVREFCELVLY
                     AQGSLAMRVAEYAEK"
BASE COUNT          500 a         1053 c         1106 g          632 t
ORIGIN      
        1 gagggccgcg agttcttcca gtccctgcgt gaggtagccg tcatcgtcca gggagtcgat
       61 cagaagggcg gccagcctgc ggtcacgctc gggcaggttg gtgagcgaca actgggctag
      121 cagatgcgcc cgcagcgtgg gcgcgtccgc ggccacctgc tggtactcgc cgtcctcggc
      181 gtcgtcgcgg tagccggatg cgggggcatc gtccagcgcg ctgaaatcgg cgttctcgcg
      241 gtcgcccggc tcggcatcag cgggcggggc ctccgtggcg ggtgtcgagg cggccacatc
      301 gtgcgggaca ttctccacgg cggtaacggg atattccctg gcctcatcgt cgcgctcgag
      361 cagggggttg tcctgcagga tacgctcgag ttcctggttc agctccagcg tggacagctg
      421 cagcagacgg atcgattgct gcagctgcgg cgtgagcgtg agatgctggg acagccggag
      481 ttggagggaa tgcttcatgc cgtgatgggt gggcttatgc cgtgttcccg tgggccgtcg
      541 gaagggtggg ttggctcgcc gttctggttg tcctctacgg caatcctggc gctccacgaa
      601 aatcgggtta cctcgccatg tgatcagccg ggtgaaagca acccgttgga ccggcactag
      661 agacggaagt gctctcccag gtaaaccttt cttacgcttt cattataaac gatttcatca
      721 ggcttgccgt aggcgagaac gccgccgtcg ttgatgatgt aagcccgatc gcagatgccg
      781 agagtctctc tcacattatg gtcggtaatc agcacgccga tgccgcgcgc cttgagaaac
      841 cctatgatct tctggatgtc cagtacggcg atcggatcga cgcccgcgaa cggctcgtcg
      901 agcaggatga agcgcggttc ggtcgcgagc gcgcgcgcga tctccacgcg gcgccgctcg
      961 ccgccggaga ggctgatcgc ggggctcgca tgcaggtggc tgacgtgcag atcgagcagc
     1021 aggctgtcga gcttgttgct gatcacctcg ggatcgtcgt tatagagttc caggatggcc
     1081 tgcacgttct cggcgaccgt gaggcgccgg aagatcgagg cttcctgcgg caggtaggag
     1141 agacccaacc gcgcgcggcg gtgcatcggc atatgagtga gccggatgtc atcgaggtgc
     1201 aattcgccgc cgtccatcgg caccagcccc accatcatgt agaaacaggt ggtcttgccg
     1261 gcgccgttcg gcccgagcag cccgatgacc tcgccgctgc gcacctcgag ggagacgtcc
     1321 ttcaccacga cgcgcgagcg gtagcgcttg gagagccgct gcgcgcgcaa ctcgtaattg
     1381 cgcgccgtgg ccggcatggc ggccgcggcc tggccggggt cgtcggccag cttgttcaga
     1441 gtgattggca tggagacagc ctggcgtgtg tttcccggcg tcacggagtc ttgcgggcgc
     1501 cggcttcctc acgcggcgtg gcgatggccg ccgcaggctt gaggctcacc ggcggcgcgg
     1561 ccggcggatt ttccttcgac ttgggctgta ttaccgcccg cactcggccc tcgggggcgc
     1621 cgggcgctgt cgcattcctg gtgccgccgg tcacctggaa gaactcggtg cgggcatcgt
     1681 acgagatgta atttccgcgc acctcgtcct gcccgcgctt caactgggcg cggtcgaaca
     1741 tctgcagggt ctcgatgcgg ccgtcgtact cgatgcgctc ggcccagcct tcgatgtact
     1801 cgtcgtagcc ctcgcgcttc tgacggaatg aagcggggct gccccacgtg gtgccctgct
     1861 tgaagcccgc cttgtcctgg cgcacttcca tgcggtcgcc gcggatctgc agggtgccct
     1921 ggctcaatac gacgttgcct tcgaaggtcg cgatctgctt tgcgtcatcg atggtcacgc
     1981 gatccgcttc caggttgacc ggcttgtcgc ggtcggcttt ttccgcggac gcgggcaggg
     2041 cgagcgcggc cagcacgcac agcgcggcca atgcgcggcc gggccggccc gtcctggctg
     2101 acgcggcgtc accgctgcgg agctttgccc ggatcatagg ttccacggac gctggagagc
     2161 agcttgatga cgcgggtttc gttgttcagc tcgaggccgt ccgccgtgac cgtcgtcgcc
     2221 gcgtcgctga gcgtaaccgc ccggtcggtc ttggccaggt tttcatccgg tatcacgtgc
     2281 aggaaggatg tgcgcacgac gagctcgctg ttcggcccgt gggccgcgcg cgtcaggcgc
     2341 acgttgtcct ggaaatagac gtgctggccg ttcgccgaaa ccaccgcctc gctggcggtg
     2401 atcgtaaccg gcgctttcgc cttgccgtag ttgacgaatt tcggcgcggt cagcaaggtg
     2461 gtatcgcctt cgggatagtg catcattttc accgccgaca gtgtatagct ggccgcgccc
     2521 gcctcgctca tgctgaccgc cgagaggccg tcgacgatgt agtcgggatc gttgcggctg
     2581 ggtccggcgc cgcgctcggg aggctgtgaa accaggtcca gccagaaagt gagcgccgcc
     2641 agcattccga cgagcagaag gggcgctgcc gtggtcaggc tctcattttt ccgcatattc
     2701 ggccacccgc atcgccagcg acccctgcgc gtacaggacg agctcgcaga attcgcgcac
     2761 ggcgccgcct cccccggggc gccgggtgac aaagtgggcc tggcggcgca ccggcgccgg
     2821 ggcgcccggc acggtcaccg ccagtccgca tcgtttcatc accggcagat ccaccagatc
     2881 gtcgccgacg taggcgcatg ccgccgcatc gacgccgcaa cggcccagca gttcccggaa
     2941 cgccgcgagc ttgtccgtgg cgccctgata cagcagctcg atgcccaggt tgcgcgcgcg
     3001 ttcctcgacg cagcgcgagc ggcggctggt gatgatggcg agctggatac cgctgtcctt
     3061 cagcattttc atgccatggc cgtcgtggac gctgaagcgc ttcagctcct cccccgaatc
     3121 ggcgtaatag agcgcgccgt cggtcagcac gccgtccacg tcgaacgcgg cgacgcggat
     3181 atttcgtgcc ctctcataga tttcctgcat atttcagttt ccagtttcca gtttccagtt
     3241 catatcgtcc gggcgtccgc accaaactgg aaactggaaa ctcgcaactc g
//
LOCUS       DBNGSG010000264         7261 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2121567, whole genome shotgun sequence.
ACCESSION   DBNGSG010000264 DBNGSG010000000
VERSION     DBNGSG010000264.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7261)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7261)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7261
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2121567"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="AC158Q_06870"
     CDS             <1..514
                     /locus_tag="AC158Q_06870"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017538.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HIR3339534.1"
                     /translation="DRLEAMGRGAEFVAPALPHYPAQAAALLDALAARHPGAAVVGSS
                     LGGYYATYLAEKHDLKAVLLNPAVRPYDLFAGHLGPQQNLYTGERYEITMRHVGELRA
                     LEVASITPARYLLIVATGDETLDYRAAVARYRGCRSIVIEGGDHGLSDFGGCLVPVLE
                     FCGVADPEKH"
     gene            829..1875
                     /locus_tag="AC158Q_06875"
     CDS             829..1875
                     /locus_tag="AC158Q_06875"
                     /inference="COORDINATES: protein motif:HMM:NF048867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Kelch repeat-containing protein"
                     /protein_id="HIR3339535.1"
                     /translation="MIGMIKLLRPCFAALLAVSILVPVAYAQKWSRLAPFPEPSEELW
                     GAGAGGKLYVFGGLAPGWKPKALVYEYDPDSDAWTKKKNMPLASHHVAAAELNGKIYM
                     FGGFKYPDSGKPGWQPIDNAWEYDPAKDAWRALAPMPTKRGSSNAVVHGGKVYVIGGA
                     SMHPGSKDAFVHPARPHRALGTTEVYDPATNTWEARSPMPTARNHAAAGMANNKIYVI
                     GGRVGAVFITGGGRGVDLVEEYDPATNQWGRLQAPMPTARSAVAWGTYGGKIYVAGGE
                     AQDSRMLSTFRAVEAFDAAKNTWTSLPPMRSPRHGLAGAFVGSRLHLVSGDAQSALSG
                     AETHVEYHDVLELP"
     gene            1989..3860
                     /locus_tag="AC158Q_06880"
     CDS             1989..3860
                     /locus_tag="AC158Q_06880"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012975.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease catalytic domain-containing
                     protein"
                     /protein_id="HIR3339536.1"
                     /translation="MNVFYEEEGSFKVGSILADQDTSLQVEAPHGKRSKIKASAVLFR
                     FEGGGLPQFMDEAHKLADEIEADFLWQCTGHQEFPFETLAREYFGRAPAPVESAGLLL
                     KLHGAPMYFYKKGKGRYRPAPPEALKAALASIERKRRDTEKRGQYVAQLMASRLPPEF
                     GPVKAQLLYKPEKQSLEWKALEEACEKLKVTPARLFERCGALPSPHDYHLDRFLFEHF
                     PRGAALPAIPVPPPPRDLPFAEVEAFSIDDVTTTEIDDALSVTKRPGGGASIGIHIAV
                     PALGIAPESALDAAARERLSTVYYPGGKVTMLPETAIDAYTLAERSERPVLSLYLEIA
                     ADGSIASRTTRVEQVRIAENLRHDALEAAFNETTLAAGRVEHRFAEELTTLWHFARSL
                     ESARRGAAAEYEPRPEYSFYVENDRVSIVRRLRGTPVDKIVSELMIQANGAWGRQLAE
                     SGTAAIYRVQGGGKVRMSTVPSGHEGLGVAAYAWTSSPLRRYVDLVNQRQLVALARGE
                     PVIYGANDERLLAAMRDFEIAHDAYAEFQRQMERYWCLRWLLQENVRTITATVIRESL
                     ARFDELPLVARVPSLPALAPGTRVELEVSEMDLLELGLRCEFRGQLAPEVRAAGAAG"
     gene            3964..4977
                     /locus_tag="AC158Q_06885"
     CDS             3964..4977
                     /locus_tag="AC158Q_06885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR3339537.1"
                     /translation="MKRMGLLPDRKVVPLRRRAKHARHAPWTAALARLDARVAELERN
                     MAALDRRMTALGKHGRMKVAVSASILFHAILIVGVSFTMPDPPKFAQQPLEVTLVNAK
                     SQAKPLKADALAQANLDGGGNTDAKRRAKSPLPVPRKAQEAAELAMAKKRVELLEREA
                     KQLMTQAKSTTAVASVPETPQPKEEPQAAPSAVEIMNRSLEIARLEASIAREWESYQQ
                     RPRRRFIGARTQEFRFARYIEDWRTKVERVGETNYPQAARDHRIYGSLVVTVSIKSDG
                     SIDRVDINRSSGQRILDAAALRIVQLAAPFARFPADIAKDTDVISITRTWLFTRSDQF
                     VAE"
     gene            5082..5906
                     /gene="aroE"
                     /locus_tag="AC158Q_06890"
     CDS             5082..5906
                     /gene="aroE"
                     /locus_tag="AC158Q_06890"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HIR3339538.1"
                     /translation="MTDQYAVIGNPVAHSLSPAIHAEFARATGQDISYSRILAPLDGF
                     KGATLAFRESGGKGLNVTLPFKQEAWRLADTHTPGALAAGAVNTLQFESGRIAGHNTD
                     GTGLARDLKDNLDCDIRGRRILLMGAGGAVYGVMEPLLRELPQRVVVANRTLEQAVAL
                     IGHFEKLQKFCSRGVTALPYGALAGSRFDIVINATSTGLVDVMPPLPRDSFAPGALAY
                     EMVYGRQTLFMRFAAECGARAVDGLGMLVEQAAESFFIWRGVRPATKPVIEMFRKA"
     gene            5991..6686
                     /gene="mtgA"
                     /locus_tag="AC158Q_06895"
     CDS             5991..6686
                     /gene="mtgA"
                     /locus_tag="AC158Q_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HIR3339539.1"
                     /translation="MKVLFRWIWHILLLALIALAAVQFWFLVHIWYWTDHNPESTAFM
                     RARLEIIREDEPGARLSHRWVPYNRISGYLKRAVVAAEDDKFMDHTGFDWEAIQKAYV
                     RNLREGEIVSGASTIPQQLAKNLFLSGRRTWWRKAQEAVITAMLETVLTKRRILEIYL
                     NVAEWGDGVYGAEAAARYHFGVSAAALTPAQAARLAVMLPSPRSYPPGRDTVYLQNRT
                     ATILGRMQYARVP"
     gene            6826..>7261
                     /locus_tag="AC158Q_06900"
     CDS             6826..>7261
                     /locus_tag="AC158Q_06900"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR3339540.1"
                     /translation="MLRRQFLAGAGALAVYGWAGAQEQADALRGPDVIFVPTPNEVVS
                     KMMELAKVTQKDTVYDLGCGDGRIVITAAQRYGARGVGIDIDPERVKEATDNVRKAGV
                     ADRVKIVRGDLFEADISAATVVTLYLLTDLNLKLRPKLLRDLK"
BASE COUNT         1318 a         2297 c         2403 g         1243 t
ORIGIN      
        1 agacaggctg gaggcgatgg gacgcggcgc ggaattcgtc gcgcctgccc tgccgcacta
       61 tccggcgcag gctgcggcat tgctggatgc gctcgccgcc cggcatcccg gcgccgccgt
      121 ggtgggcagc tcgctcggcg gctattacgc gacctatctc gccgagaagc acgatctcaa
      181 ggccgtgctg ctcaatcccg cggtgcggcc gtacgatctc ttcgccggcc atctcgggcc
      241 gcagcaaaat ctctataccg gcgagcgcta cgagatcacg atgcggcacg tcggggaatt
      301 gcgcgcgctg gaggtcgcca gcatcacgcc cgcccgctac ctgctgattg tcgccaccgg
      361 cgacgaaacg ctcgactacc gcgcggcggt tgcgcgttac cgggggtgcc gcagcattgt
      421 gatcgagggc ggcgaccacg ggttgagcga tttcggcggt tgcctggtgc ccgtgctgga
      481 attctgcggc gtggcagatc cggagaagca ttagccacgg aaacacacgg aagtaaaaga
      541 aagcgagccg ggaattcagg aaatacattc atcccattca ggcctttggt tctctatgcg
      601 ccgtcctcct gtttcagaat gccattgttg atcttgttcc gtgtgattcc gtgtgtttcc
      661 gtggccgagt tgctcttgtc aggctttgaa cagacttcag ccatgccggg tcggcatgcc
      721 gttgcccgca gctcgggatt gggcgtagca tgaaagcccg tgcgtgagga gcgtgctcga
      781 caggaaccgc acacaagaat gcggtctcat tttcaggagg aggttccgat gatcggtatg
      841 atcaagttgt tgcgtccgtg cttcgctgcg ctgctggcgg tatcgattct agttccagtc
      901 gcctatgcgc agaagtggag caggctcgcc cccttcccgg agccctcgga agaactctgg
      961 ggcgccggcg ccggcggcaa gctttatgta ttcggtggac tcgcgccggg ctggaagcca
     1021 aaggccctgg tctatgagta cgacccggac agcgatgcct ggaccaagaa gaagaacatg
     1081 ccgctcgcct cgcatcacgt cgccgctgcc gagctgaatg gaaaaatcta catgttcggg
     1141 gggttcaagt atccggactc gggcaaaccc ggatggcagc cgatcgacaa cgcatgggag
     1201 tacgaccctg cgaaggatgc ctggagggcg ctcgcgccga tgccgaccaa gcgcgggtcc
     1261 tccaatgcgg tggtgcatgg cggaaaggtt tatgtgatcg gcggcgcatc catgcaccct
     1321 ggctccaagg atgccttcgt gcatccggcg cgtccgcatc gcgcgctggg taccaccgag
     1381 gtctacgatc ctgcgaccaa cacttgggag gcccgaagcc cgatgcccac cgcgcgcaat
     1441 cacgcggccg caggaatggc aaacaacaag atttacgtca tcggcggccg cgtcggcgcg
     1501 gtgttcatca cgggaggagg gcggggtgtt gatctcgtgg aggagtatga cccggcgacc
     1561 aaccaatggg gccgcttgca agcaccgatg cctacggcaa ggagcgccgt ggcatggggc
     1621 acttatggcg gcaagatcta tgtggcgggt ggggaagcgc aggactcccg catgttgagt
     1681 acattccgcg cggtcgaggc gttcgatgcg gcgaaaaaca cctggacgag cctgccaccg
     1741 atgcggtccc cccgccacgg gcttgccggg gcgttcgtcg gcagccggct gcatctcgtc
     1801 agcggtgacg cacagtctgc cctgagtgga gcggagaccc acgtggaata ccacgatgta
     1861 ctggaacttc cgtaagagtt gagcttcgtc acacgcgggt tgggccgcga cgtcgcttat
     1921 aattacgggc gcagccgttc gagccgcgcc cgttttttca ctccggcagc ccctcccgac
     1981 cgtcccagat gaacgttttt tacgaagaag aaggctcgtt caaggtcggc agcattcttg
     2041 ccgaccagga cacctctttg caggtggaag ccccgcacgg caagcgttcc aagataaagg
     2101 cttccgccgt gctgttccgt ttcgaagggg gcggcctgcc gcagttcatg gacgaggcgc
     2161 acaagctcgc ggatgagatc gaagcggatt tcctgtggca atgcaccgga caccaggagt
     2221 tcccgttcga gacgctcgca agggaatact tcgggcgcgc gccggcaccg gtggaatcgg
     2281 cgggcctgct gctgaagctg cacggtgccc cgatgtactt ctacaagaaa ggcaagggcc
     2341 gctacaggcc ggcgccgccg gaagcgctga aggccgcgct cgcctcgatc gagcgcaaac
     2401 gccgcgatac ggaaaagcgc gggcaatacg tggcgcaact gatggcctcg agactgccgc
     2461 cggaattcgg gccggtcaag gcgcaactgc tctacaagcc cgagaagcag agcctggaat
     2521 ggaaggcgct cgaagaggcg tgcgagaagc tcaaggtgac gccggcgcgg ctgttcgagc
     2581 gctgcggtgc actgccatca ccgcacgact atcacctgga ccgcttcctg ttcgagcact
     2641 tcccgcgcgg cgcggcgctg cccgccatcc cggtgccgcc gccgccccgg gacctgccgt
     2701 tcgccgaagt cgaggctttc agcatagacg acgtcacgac caccgaaatc gacgatgcgc
     2761 tttcggtgac gaagcggccg ggcggggggg cctccatcgg catccatatc gccgtgcccg
     2821 cgctcggcat tgcgccggaa tccgcgctgg acgccgccgc ccgcgagcgc ctgtccacgg
     2881 tttactaccc ggggggcaag gtcaccatgc tgccggagac ggcgatcgat gcctacacgc
     2941 tggcagagcg cagcgagcgc cccgtgctgt cgctgtacct cgaaatcgcg gctgacggct
     3001 ccatcgcgtc gcggacgacg cgcgtcgagc aggtgcgcat cgccgagaat ctccgccacg
     3061 acgcgctgga ggccgcgttc aacgaaacga cgcttgccgc ggggcgcgtc gagcaccgtt
     3121 tcgcggagga actcaccacg ctgtggcatt tcgcccgctc gctggagtcg gcgcggcgcg
     3181 gcgctgccgc cgaatacgag ccgcgtcccg aatacagttt ttacgtcgag aacgatcgcg
     3241 tgagcatcgt gcggcggttg cgcggcacgc cggtcgacaa gatcgtctcc gagttgatga
     3301 tccaggcaaa cggcgcctgg ggccgccagc tggcggagag cggcaccgcg gccatctacc
     3361 gcgtgcaggg cggcggcaag gtcaggatga gcacggttcc ttcggggcac gaagggctcg
     3421 gcgtcgcagc ttacgcgtgg acgagctcgc cgctgcgccg ctacgtggac ctcgtgaacc
     3481 agcgccagct ggtcgcgctc gcgcgcggcg agccggtcat ctacggcgct aatgacgagc
     3541 ggctcctggc ggcgatgcgg gatttcgaga tcgcgcacga tgcgtatgcc gagttccagc
     3601 gccagatgga gcgctactgg tgcttgcgct ggctgctcca ggaaaacgtc cggacaatta
     3661 ccgccaccgt gatccgcgag agtctggcgc gcttcgacga gttgccgctg gtcgcgcgcg
     3721 tgccctcgct gccggcgctg gctcccggca cccgggtcga gctcgaggtg tcggaaatgg
     3781 acctgctgga actcggcttg cgttgcgagt ttcgggggca gctggcgccc gaagtgcggg
     3841 cggcgggggc ggcgggctag cgtcccctgg attaccgcat attcgcgatt tcacattaga
     3901 tttttcaggc aaccccccgt taataaaagc atttctgtgc aggattatta gaggtacgct
     3961 agaatgaagc gcatggggtt gttaccggat cggaaagtcg tgccgctcag gcgacgggcg
     4021 aagcacgcgc gccacgcgcc gtggacagcc gcgcttgcgc ggctcgatgc gcgcgtcgcc
     4081 gaactcgagc gcaacatggc cgcgctcgac cggcgcatga ccgcgctcgg caaacacggg
     4141 cgcatgaagg ttgccgtgtc cgcgtcaatc ctgtttcatg ccatcctcat cgtgggcgtc
     4201 agcttcacga tgcccgaccc gcccaagttc gcgcagcaac cgctggaagt gacgctggtc
     4261 aacgccaaga gccaggccaa accgctgaag gccgatgcgc ttgctcaagc caatctcgac
     4321 ggcggcggca ataccgacgc caagcgccgc gccaagagcc cgttgccggt gccgcgcaaa
     4381 gcacaggagg cggccgagct cgccatggcg aagaagcgcg tggagttgct ggagcgcgag
     4441 gcgaaacagc tgatgaccca ggcgaaatcg acgacggccg tcgcctcggt gcccgagacg
     4501 ccgcagccga aggaagaacc gcaggccgcg cccagcgcgg tcgagatcat gaaccggagc
     4561 ctggagatcg cgcggctgga ggcgagcatc gcgcgcgagt gggagagcta tcagcagcgg
     4621 ccgcggcgtc gtttcatcgg cgcgcgcacc caggaattcc ggttcgcgcg ctacatcgag
     4681 gactggcgca ccaaggtcga gcgcgtgggc gagacgaatt atccccaggc cgcgcgcgac
     4741 cataggatct acggcagcct ggtcgtgacc gtgtcgatca agtccgacgg ctcgattgat
     4801 cgcgtggaca tcaaccgttc ctcggggcag cgcattctcg acgcggctgc gctgcgcatc
     4861 gtccagctcg cggcgccgtt cgcgcggttt cccgccgaca ttgccaagga caccgacgtc
     4921 atcagcatca cccggacctg gctcttcacg cggtcggatc agttcgtcgc tgaataacag
     4981 tttcaagata caaggctcaa gatccaagta aagctgcgca cagccgcgtg cgcccggtga
     5041 tacagtcttg taacttgcat cttgaaactt gagtctgttg catgactgac caatatgccg
     5101 ttatcggcaa ccccgtcgcg cacagcctgt caccggcgat ccatgccgaa ttcgcgcgcg
     5161 cgaccgggca ggacatctcc tatagccgaa tccttgcgcc gctcgatggc ttcaagggag
     5221 caacgctggc attccgcgag agcggcggca aggggctcaa cgtcacgctg ccgttcaagc
     5281 aggaggcctg gcggcttgcc gacacgcata cgcccggcgc gctcgctgcg ggcgcggtca
     5341 acacgctcca gttcgagagc gggcgcatag ccggccacaa taccgacggg accgggttgg
     5401 cgcgcgatct caaggacaac ctggactgcg acatccgcgg ccggcgcata ctgctgatgg
     5461 gggccggcgg cgcggtctat ggtgtgatgg agccgttgct gcgcgaactc ccgcagcggg
     5521 tggtcgtcgc caaccgcacg ctggaacagg cggtcgccct catcgggcat ttcgagaagc
     5581 tgcagaaatt ctgctcccgc ggcgtcacgg cgctgcccta cggggcgctg gcgggctcgc
     5641 gcttcgacat cgtcatcaac gccacttcta cgggcctcgt cgatgtcatg ccgccgctgc
     5701 cgcgcgacag tttcgcgccg ggcgcgctcg cctacgagat ggtctacggc aggcagacgc
     5761 tcttcatgcg gttcgcggcc gagtgcggcg cgcgcgcggt ggacggtctg ggcatgctgg
     5821 tcgagcaggc cgccgaatcg tttttcatct ggcgcggcgt gcggccggcg acgaagccgg
     5881 tcatcgaaat gttcagaaag gcgtgattcg tgatttgtga ttcgtgagtc gtccaacgcc
     5941 atgaagacgg ttcactcgat tcgcgattca cgaatcacaa ttcacgcact ttgaaagtcc
     6001 tcttccgctg gatttggcac atcctcctgc tggcgctgat cgcgctggcg gcggtgcagt
     6061 tctggttcct cgtgcacatc tggtactgga cggatcacaa tcccgaaagc accgcgttca
     6121 tgcgcgcccg gctggaaatt atccgcgagg atgagcccgg cgcccgcctg tcccaccgct
     6181 gggttcccta taaccgcatc tcgggatacc tcaagcgggc ggtcgtcgcc gctgaagatg
     6241 acaagttcat ggaccatacc ggctttgact gggaagcgat ccagaaagct tacgttagga
     6301 acctgcggga aggagagatc gtctccggcg cgtccacgat cccgcagcag ctcgcgaaaa
     6361 acctgtttct ctcgggccgc cgcacctggt ggcgcaaggc gcaggaggcg gtgatcacgg
     6421 cgatgctgga aaccgtgctg accaagcgcc gcatcctcga gatctatctc aacgtcgccg
     6481 agtggggcga cggcgtgtac ggcgcggagg ccgctgcgcg ttaccacttc ggggtgtccg
     6541 cggcggcgct gacgccggcc caggcggccc ggcttgccgt catgttgcca agcccgcgca
     6601 gctatccgcc aggccgcgac accgtgtacc tgcaaaaccg caccgcgacg atactcgggc
     6661 gcatgcagta cgcccgggtg ccctgaaggg agggcagagt gtcgaggagt gagtgtcgag
     6721 tgaagagagc ataagcgggt tacccctcga ccctcgactc ccggcactcg accctggtcg
     6781 ggtagaatgt cccgccctga ttaaatcatc gattggagat tcctcatgct aagacggcag
     6841 tttctagcag gcgccggtgc cctggccgta tatggctggg ccggcgccca ggagcaggcc
     6901 gatgccctgc gcggccccga cgtcattttc gtgccgacgc caaacgaagt cgtgagcaag
     6961 atgatggagc tggcgaaagt gacccagaaa gacacggtct acgatctcgg gtgcggcgac
     7021 ggccgcatcg tgatcaccgc cgcgcagaga tacggcgcgc gcggcgtcgg catcgacatc
     7081 gatcccgagc gggtgaagga agcgacggat aacgtgcgca aggccggcgt ggccgaccgg
     7141 gtgaagatcg ttcgcggcga cctgttcgag gccgacatca gcgccgcgac ggtggttacc
     7201 ctctatctcc tcaccgacct caatctcaag ctgcgcccga agctgctgcg cgacttgaag
     7261 c
//
LOCUS       DBNGSG010000265         2402 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4329501, whole genome shotgun sequence.
ACCESSION   DBNGSG010000265 DBNGSG010000000
VERSION     DBNGSG010000265.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4329501"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="AC158Q_06905"
     CDS             <1..864
                     /locus_tag="AC158Q_06905"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR3339541.1"
                     /translation="EWLEKNAPRAVRCSITGYGSSGPDSALPGYDFILQAESGLMSIC
                     GEPDGTPTKYGVAIVDVVTGLYACNAILGALAARERTGRGQHVEVCLYDSGIAMLINV
                     ASNYLVSGKDARRFGNGHPSIVPYTTYPTADGMIAVAVGNDGQFARFAETIGRGEWAR
                     DARFARNPDRVTNREALDGLIAQALESGKTAAWIEKLRAAGVPCGPINTVAEALTDPH
                     TLARDMVRTIRHPKVDELKMVGIPFRMNGTPPTIRRAPPLLGEHTDEVLTGELGFSAE
                     RIAQLRAEKVV"
     gene            1037..1480
                     /gene="mce"
                     /locus_tag="AC158Q_06910"
     CDS             1037..1480
                     /gene="mce"
                     /locus_tag="AC158Q_06910"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HIR3339542.1"
                     /translation="MITGLSHVSIVVPSLEAAAKRLKELYGLEIGAAKVNEQQGVRLA
                     YVELPNARIELMEPSRPDSPVSKFLERNPAGGIHHFCLGVDDVPAAAAGIAARGARVL
                     GEGKEQRNVHGERIAFVHPKDFLGALVELEESQSKIESRKSKGKT"
     gene            1477..>2402
                     /locus_tag="AC158Q_06915"
     CDS             1477..>2402
                     /locus_tag="AC158Q_06915"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3339543.1"
                     /translation="MKIFCTLLAGVFFLLSPFAFRLESAALAAGYPERPVRMLIPFAP
                     GGATDIIARMIEPRMSRALGQQVVVDNRPGAAGNIAVELTAQAQPDGYTLLVGNISTN
                     SINPILFAGRTKVNAVRDLAGITKLVAIPNFMLGSPKVPGTTLKEAIEYARSRQGQLN
                     FGAPLGSYAHLDMLAFTARAGIRMTHIPTKGAGETLANLLRGDSHIQISNVASNIGPV
                     RAGQIKAYAVTSEKRLADMPDVPTMAEAGFAGIGSLNWNGLFAPVRTPKPVLARLHQV
                     SVAAMKELESEGVLAKRQTPMSLSSSPAEFDA"
BASE COUNT          439 a          764 c          801 g          398 t
ORIGIN      
        1 gaatggctgg aaaagaacgc cccgcgcgcc gtgcgctgct cgatcaccgg ttacggatcg
       61 agcggccccg attccgcgtt gccgggctac gacttcatcc tgcaggcgga atccggcctg
      121 atgagcatct gcggcgagcc cgacggcacg cccacaaagt acggcgtggc gatcgtggac
      181 gtcgtgacgg ggctttacgc ctgcaacgcc atccttgggg cgctcgcggc gcgggaacgc
      241 accggccgcg gccagcacgt cgaagtctgc ctctacgatt cgggcatcgc gatgctgatc
      301 aacgtcgcgt cgaactacct ggtctcgggc aaggacgcgc gccgtttcgg caacgggcac
      361 cccagcatcg tgccgtacac gacctacccg accgccgatg gcatgatcgc ggtggcggtg
      421 ggcaacgacg gccagttcgc gcgcttcgcc gaaaccatcg gccgcggcga atgggcgcgc
      481 gatgcccggt tcgcgaggaa ccctgaccgc gtcacgaacc gcgaagcgct cgacgggttg
      541 atcgcgcagg cgctggaatc cggaaagacc gcggcctgga tcgagaaact gcgcgcggcc
      601 ggcgtgccgt gcggaccgat caacacggtg gcggaggcgc tcaccgaccc gcacacgctg
      661 gcacgcgaca tggtgcgcac catccggcat cccaaggtgg acgagctcaa gatggtgggc
      721 atccctttcc gcatgaacgg cacgccgccg acgatacggc gcgcgccgcc gctgctgggc
      781 gagcataccg acgaagtgct gaccggggag ctgggtttca gcgcggagcg gatcgcgcaa
      841 cttcgcgcgg agaaagtcgt ttaatagaca ggattaacag gattaacaag attcaatcaa
      901 tcaaatcggt ttctgttctg ctttttgatc tatttctgta aatcttgtat atccccttcc
      961 cccgtcttgg gggaaggtag gatgggggca gctgtctatt gcttttgacg ttggcgggtc
     1021 cttgccagga gcggtgatga ttaccggatt gagtcatgtg tctattgtcg tgccgagcct
     1081 ggaagcggcg gcgaagcggc tgaaggagct gtacggcctc gagatcggcg cggcaaaagt
     1141 gaacgagcag cagggcgtgc gcctcgccta cgtcgaactg ccgaacgccc gcatcgagct
     1201 gatggaaccg tcgcgccccg attcgccggt gtcgaaattc ctggagcgca accccgcggg
     1261 cggcatccac catttctgcc tcggcgtgga cgacgtgccc gccgccgcag ccggcatcgc
     1321 ggccaggggc gcgcgcgtgc tcggggaggg aaaggaacag cgcaacgtgc acggcgagcg
     1381 catcgctttc gtgcacccga aggattttct gggggcgctg gtcgagctcg aggagagcca
     1441 gtcgaagatc gaaagtcgaa agtcgaaagg aaaaacatga aaatcttttg cacgctcctt
     1501 gccggtgtct tcttcctcct ttcgccattc gcctttcgcc ttgagtcggc ggcgctcgcc
     1561 gccggttacc cggagcggcc ggtgcggatg ctgattccgt tcgcgccggg cggcgccacc
     1621 gacatcatcg cgcgcatgat cgagcccagg atgagccggg cgctgggcca gcaggtcgtc
     1681 gtggacaacc gcccgggcgc ggccggcaac atcgcggtgg agctgacggc gcaggcgcag
     1741 cccgacggct acacgctgct ggtcggcaac atctccacca actcgatcaa cccgattctg
     1801 ttcgccggca ggacgaaggt aaatgccgtc agggatctcg ccggcatcac caagctggtg
     1861 gcgatcccca atttcatgct gggcagcccg aaagtgccgg gcaccacgct gaaggaggcg
     1921 atcgaatatg cccggtcgcg gcaggggcag ctcaatttcg gcgcgccgct gggttcctac
     1981 gcgcacctcg acatgctggc attcacggcg cgggcgggga tccgcatgac gcacatcccg
     2041 accaaggggg cgggcgagac actggcgaac ctactgcgcg gcgattccca catccagata
     2101 tccaacgtcg cgtccaatat cggcccggtg cgggcgggac agatcaaggc ttacgcggtc
     2161 acttcggaga agcggctcgc cgacatgccc gacgttccca ccatggcgga ggccggcttt
     2221 gcgggcatcg gcagcctgaa ctggaacggc ctgttcgcgc cggtccgcac gccgaagccg
     2281 gtgctcgcca ggctgcacca ggtgtccgtc gcggcgatga aagagctgga gtcggaaggc
     2341 gtgctcgcca agcgccagac cccgatgagt ctcagttcct cgccggccga gttcgatgcc
     2401 ta
//
LOCUS       DBNGSG010000266         4737 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4670146, whole genome shotgun sequence.
ACCESSION   DBNGSG010000266 DBNGSG010000000
VERSION     DBNGSG010000266.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4737)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4737)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4737
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4670146"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="AC158Q_06920"
     CDS             <1..192
                     /locus_tag="AC158Q_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012364442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339544.1"
                     /translation="GKAQSYLEASLAIEPTYSAHLALARLHERLGSPDAADRHYRESL
                     GLALAQLSSITGGRRRTPH"
     gene            complement(208..897)
                     /locus_tag="AC158Q_06925"
     CDS             complement(208..897)
                     /locus_tag="AC158Q_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HIR3339545.1"
                     /translation="MGHREFHGSARSGWLRAAVLGANDGLISTSSLVVGFASAQAAPE
                     SILLAASAGLVAGAFSMAAGEYVSVSSQADAERADLAREKHELATDPAKERAELAAIY
                     VGRGLTQKLANQVSDQLMAKDPLAAHARDELGIVETTRARPLQAAIASAAAFSVGAAL
                     PLVLTAIVPARVLSLAVIGGTLLLLACLGAVAARLGGAPVLAGALRVTLWGAVAMAGT
                     ALVGMLFGTAP"
     gene            complement(935..1948)
                     /locus_tag="AC158Q_06930"
     CDS             complement(935..1948)
                     /locus_tag="AC158Q_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006413312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HIR3339546.1"
                     /translation="MPHQVTIQASGHSFTVNDGETVLAAALREGIVIAYGCRHGVCGT
                     CKGKLVEGAVDYGTYQEHALPAEEKDAGMALFCQARPRSDLLIQCRELGAAVKGMQIR
                     MMPARVMKMERAAPDVMLLELKLPANEKFMFLAGQYVDLILRDGTRRSFSMANAPHDN
                     AFLQLHLRNYGGPFSQHAFGAMKEKDILRIEGPFGTFFLREEGAKPIVLLASGTGFAP
                     IKSLVEHALHTGIKRPMTLYWGCRVRADLYRNELAEGWQRDGILRYVPVLSEATAPDH
                     WAGRTGFVHRAVMEDFPDLSGHQVYACGAPIVVESAHRDFTAQCGLPEDEFFSDSFTP
                     SVP"
     gene            2143..2472
                     /gene="cutA"
                     /locus_tag="AC158Q_06935"
     CDS             2143..2472
                     /gene="cutA"
                     /locus_tag="AC158Q_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HIR3339547.1"
                     /translation="MDDIVIVLTNLPDRAAAVKLAHELVARRLAACVNVLAECTSVYR
                     WKGGIENAAEVPVLIKTRAGRYAEVEAAIRELHPYELPEIVAVPVQRGFDEYLQWVTD
                     ETAIAIG"
     gene            2598..4379
                     /gene="dsbD"
                     /locus_tag="AC158Q_06940"
     CDS             2598..4379
                     /gene="dsbD"
                     /locus_tag="AC158Q_06940"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     [NAD(P)H] activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HIR3339548.1"
                     /translation="MRAILILLLVLCGSALAAEPELLEPDKAFRFAARLKDERTIEVT
                     YQIAPGYYLYRDKFRFTLTPADAKPGAPQMPAGKKHRDEFFGEVETYRGGLSILLPFE
                     SADAKVIQLAALSQGCADIGVCYVPHEQKAELRLAALGPGGVRADAPGMTFGGPSAPG
                     EDDRVAALLKGGFWFAIASFFGFGLLLSFTPCVLPMVPILSSVIVGAGHPVTKTKGFL
                     LAIAYVLGMAITYAVAGVAAGLSGAMLSAALQNPWVVGAFAAMFVVLAASMLGAYELQ
                     LPVALQSRIAAASGRFHGGHAAGVFSMGVLSALILGPCVAAPLAGALLYISQSRDVVL
                     GGTALFAMALGMGVPLLVIGASAGALLPKAGPWMDTVKRFFGVLLLGVAIYLVSPFIA
                     VSVQMFLWGALLIGMAVYLKVFDSLPPGTHGFQRAAKGIGVLALIAGAAYVVGAFSGA
                     RDILRPLSGLRESATGAASAHETPFQKVRNLAELDAAIRAAAGRTVMLDFYADWCVSC
                     QEMERYTFSDERVKDRFRDMALLQADVTANNADDAALLKRFRLFGPPGIVFFDREGRE
                     IQGLRVIGFQPADRFVTVLDQALGMRR"
     gene            4376..>4737
                     /locus_tag="AC158Q_06945"
     CDS             4376..>4737
                     /locus_tag="AC158Q_06945"
                     /inference="COORDINATES: protein motif:HMM:NF012787.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HIR3339549.1"
                     /translation="MKKAHALAALAVVAIAAAAGGAAYHGWSRDNAAARAAVEQVYGS
                     RLADLQGGIQPLARWRGQVLVVNFWATWCAPCREEIPGVVRLQQRYGARGLTFVGIAI
                     DQPDKVAEFAREFRINYPL"
BASE COUNT          730 a         1560 c         1636 g          811 t
ORIGIN      
        1 ggcaaggccc agagttatct cgaggcgagc ctcgcgatcg agccgactta ctccgcccac
       61 ctcgcgctgg cgcggctgca cgagcggctg ggcagcccgg acgcggcaga ccggcattat
      121 cgcgagagcc tcgggctcgc gctggcccag ttgagcagta tcaccggcgg ccgccgtcga
      181 acaccgcact aacagggcac ccggaagtta gggcgccgtg ccgaacagca ttccgaccag
      241 ggcggtgccg gccatcgcga cagcgcccca gagagtgacc cgcagcgcgc cggccagaac
      301 cggggcgccg cccagccggg cggcaacggc gcccaggcaa gcgagcagca gcagcgtgcc
      361 gccgatcacg gcgagggaaa gtaccctggc ggggacgatc gctgtcagga ccagcggaag
      421 cgccgcgccg acggaaaacg ccgcagccga ggcgatcgcg gcctggaggg ggcgcgcgcg
      481 cgtcgtctcg acgatgccaa gctcgtcccg ggcgtgggcg gccagcggat ctttggccat
      541 gagctgatcg gacacttggt tggcgagctt ttgcgtgagg ccgcggccga cgtagatggc
      601 agcgagctcg gcccgttcct tggcgggatc ggtggcgagc tcgtgcttct cgcgcgcgag
      661 atccgcccgc tctgcgtcag cttgcgaact caccgagaca tactcgccgg ccgccatcga
      721 aaaagcgccc gccaccaggc cggcggacgc ggccagcagg atgctctcgg gcgcggcctg
      781 ggccgaagcg aaaccgacga cgaggctgct ggtggatatc agaccgtcgt tggctcccag
      841 caccgcggcc cggagccagc cgctgcgggc gctgccatga aattccctgt gacccatgat
      901 tctccggtgt cgagccttcg gctgatgttc aacgctacgg cacggaaggc gtgaaggagt
      961 cggagaagaa ttcgtcctcc ggcagcccgc actgcgccgt gaaatcccgg tgcgcggatt
     1021 ccaccacgat cggggcgccg caggcgtaga cctggtggcc ggacaggtcg gggaagtcct
     1081 ccatcaccgc ccggtgcacg aatcccgtgc gcccggccca gtggtcaggg gccgttgcct
     1141 ccgagagcac cggcacgtag cgcagaatgc cgtcgcgctg ccagccctcg gcgagctcgt
     1201 tgcggtacag gtcggcgcgc acccggcagc cccagtagag cgtcatcggg cgcttgatcc
     1261 cggtgtgcag cgcgtgctcg acgagagact tgatcggcgc gaaaccggtg ccgctcgcga
     1321 gcaggacgat gggcttcgcg ccctcctcgc gcaggaagaa agtgccgaac ggcccctcga
     1381 tgcgcagtat gtctttttct ttcatcgcgc cgaacgcgtg ctggctgaac ggcccgccgt
     1441 agttcctgag gtgcagctgc aggaacgcgt tgtcgtgcgg cgcgttcgcc atcgagaaac
     1501 tgcggcgcgt gccgtcgcgc aatatgaggt ccacgtactg gccggcgagg aacatgaact
     1561 tttcgttcgc cggaagcttc aactcgagca gcatgacgtc cggcgcggcg cgctccattt
     1621 tcatcacgcg cgcgggcatc atccggattt gcatgccctt taccgcggcg ccgagctcgc
     1681 ggcactggat caggaggtcg gagcgcggcc tggcctggca gaacagcgcc atgccggcgt
     1741 ctttctcctc ggcgggcagg gcgtgctcct ggtaggtgcc gtaatccacg gcgccctcga
     1801 ccagcttgcc cttgcacgtg ccgcagacgc cgtggcggca gccgtaggcg atgacgatgc
     1861 cctcgcgcag cgcagccgcg aggacggttt caccgtcgtt gacggtaaac gagtgtccac
     1921 ttgcctggat cgtgacctga tgcggcatgg gcctttcaat atgaggggat actcccctca
     1981 tgctccctac attaggggct gtttcctgca tatgtgaaac agcctcgtac cgtaccgccg
     2041 ccccgggacg gcggcgcact taagttaaaa ttcccctatg acaccggggc ggggcagcag
     2101 tctatcacgg cggccggacg ggatccgtga ggagggcaat ccatggacga catcgtgatc
     2161 gtgctcacca atcttcccga ccgcgccgcg gcggtgaagc tcgcgcacga actcgtcgcc
     2221 aggcggctcg ccgcgtgcgt gaacgtgctc gcggaatgca cctcggtcta tcgctggaag
     2281 ggggggatcg agaacgccgc cgaagtgccg gtgctgatca agacgcgcgc gggacgctat
     2341 gccgaggtgg aggccgcgat ccgtgaactg catccctacg aacttcccga aatcgtcgct
     2401 gtcccagtgc agcgcggttt cgacgagtac ctgcaatggg tgacggacga aaccgccatc
     2461 gccatcggtt gatgcaatgc cccagtcgca aaaatacagc ggtcggaagg gaacccggtt
     2521 cgtgatcgcg aacagcgggg atggcattgc attactgaac gatatcttga ttacgagacg
     2581 tgctcgttac atctagcatg cgtgcgattc tgatcctttt gctggtgctg tgcggctccg
     2641 cactcgcggc ggagccggag ctgctcgagc ccgacaaggc gttccggttc gctgcacggc
     2701 tgaaggacga gcgcacgatc gaggtcacct accagatcgc gccgggttat tacctctacc
     2761 gcgacaagtt ccgcttcacg ctcacgcccg cggacgcgaa gcccggcgcg ccgcaaatgc
     2821 cggccggtaa aaaacaccgc gatgagttct tcggcgaggt cgagacctat cgcggcggcc
     2881 tgagtatcct gctgccgttc gagtccgcgg atgcgaaggt cattcagctt gccgcgctgt
     2941 cgcagggctg cgcggacatc ggcgtgtgct acgtgcccca cgagcaaaag gccgagttgc
     3001 ggctggctgc gctcggcccg gggggcgtgc gcgccgacgc cccgggcatg acgttcggcg
     3061 ggccgtccgc gccgggagag gacgatcggg tcgcggcgct gctcaaaggc ggtttctggt
     3121 tcgccatcgc aagctttttc ggctttggcc tgctgctctc gttcacgccg tgcgtgctgc
     3181 cgatggtgcc gatcctctcg agcgtcatcg tcggcgccgg gcatcccgtc accaagacga
     3241 aaggcttcct cctcgctatt gcctatgtgc tcggcatggc gattacctac gcggtggcgg
     3301 gcgtcgcggc gggactgtcc ggggcgatgc tctcggcagc gctgcagaat ccctgggtgg
     3361 tgggcgcgtt cgccgcgatg ttcgtggtcc tcgcggcctc gatgctcggc gcctacgagc
     3421 tgcaactgcc ggtggcgctg cagagccgca tcgcggccgc cagtggccgc tttcacggcg
     3481 ggcacgcggc gggcgtgttc tcgatgggcg tgctctcggc gctgatcctc ggcccttgcg
     3541 tggccgcgcc gctcgcgggc gcgttgctct acatcagcca gagccgcgat gtcgtgctgg
     3601 gtggcaccgc cctgttcgcg atggcgctcg gcatgggcgt gccgttgctc gtgatcggcg
     3661 cctcggccgg cgcgctgctg ccgaaggcgg gaccgtggat ggacacggtc aaacgcttct
     3721 tcggcgtgct gttgctgggc gtggcgatct atctggtttc ccccttcatt gccgtttcgg
     3781 tgcagatgtt cctgtggggc gcgctgttga tcgggatggc ggtctacctg aaggtgttcg
     3841 attcgctgcc gcccggcacg cacggcttcc agcgcgcagc caagggcatt ggcgtgctcg
     3901 cgctgatcgc cggcgccgcc tacgtggtcg gggcgttctc gggtgcccgc gatatcctgc
     3961 ggccgctctc gggcctgcgc gaatcggcca ccggcgccgc cagcgcacat gagaccccgt
     4021 tccagaaggt aaggaatctg gccgagctcg acgcggcaat ccgggccgcg gcggggcgca
     4081 cggtgatgct ggacttctat gccgactggt gcgtgtcgtg ccaggaaatg gagcgctata
     4141 ccttcagcga cgagcgcgtg aaggaccggt tccgcgacat ggcgctgctg caggccgacg
     4201 tcaccgccaa caatgccgat gacgccgcgc tgctgaagcg attccgcctg ttcggtccgc
     4261 ccggcatcgt tttcttcgac cgcgagggcc gcgagatcca gggcctgcgc gtgatcgggt
     4321 tccagcccgc cgaccggttc gtgacggtgc tcgatcaagc cctcggtatg cgccggtgaa
     4381 gaaagcgcat gcactcgcgg cgctggccgt ggtggcgatt gccgccgcag cggggggcgc
     4441 ggcttatcac gggtggtcgc gcgacaacgc cgctgcccgc gccgccgtcg agcaggtcta
     4501 tgggtcacgt ctcgccgatc tccagggcgg catccagccg ctggcgcggt ggcgcgggca
     4561 ggtgctggtg gtcaatttct gggccacctg gtgcgccccg tgccgcgagg agatacccgg
     4621 agtcgtcagg ctccagcagc gctacggcgc ccgcggactg acgttcgtcg gcatcgccat
     4681 cgaccagccg gacaaggtcg ccgagtttgc ccgcgagttc cggatcaact accccct
//
LOCUS       DBNGSG010000267         4651 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4330108, whole genome shotgun sequence.
ACCESSION   DBNGSG010000267 DBNGSG010000000
VERSION     DBNGSG010000267.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4651)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4330108"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..1520)
                     /gene="tcuA"
                     /locus_tag="AC158Q_06950"
     CDS             complement(39..1520)
                     /gene="tcuA"
                     /locus_tag="AC158Q_06950"
                     /inference="COORDINATES: protein motif:HMM:NF006130.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HIR3339550.1"
                     /translation="MKFEYDVVVVGKGNAALCAALSARDQDAKVAMLEAASEEEHGGN
                     SRFAGGVMRFAYGSLEDLRRVTDISDEEAANSDFGTNTREEYLDVLYRLTSFRTDPDL
                     SEMLVNGSLDTMAWLRSKGARFILNHGRQSGMVGAKRKYFGSMPIEVNGGGAGLVQYL
                     DAAAKKAGIEVFYETRAASLLYDGERVTGVRAQHKGRAAEFRAKSVVLASGGFEANPE
                     MRARYLGPGWELAKVRGSRFNVGDGLRMALDIGAAPCGNWSGCHATGWDRYAPEFGDV
                     NVGDQFQKHSYIFGLLINAEGRRFVDEGADFHSFTYAKYGGEVLKQPGQFAWQVFDSK
                     VTKLLRSEYRIKFVTKESANTLEELAPKLEGVNAEQFLKTVRDYNAAVKKDVPFDHTV
                     KDGKCTVGLSPAKSNWAQPLDTPPFDAYATTCGITFTFGGLRIDKTTGQVLDVHFHPI
                     PGLYTAGEMVGGLFYFNYPSGTGLVSGAVFGRIAGTGAGKAAK"
     gene            complement(1691..3298)
                     /locus_tag="AC158Q_06955"
     CDS             complement(1691..3298)
                     /locus_tag="AC158Q_06955"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3339551.1"
                     /translation="MEGGAKLNLSAALGLRSKIALVALVLLVIPWVGYSYVKSVERLL
                     RENQQRQIVATARAVATALQDRPGLMELRAAPPAPEGPPSPAASAAMEEVRLLLAGLA
                     RSGVRIWVLDARLNLVAIAGSLDAPPPPAADDATFGPLERLVHRVLQPVFKRVLGTPA
                     PPAEEFIPNDVVLGGREVERALDGSPAWRMRPAPAGSGVIFSAAHPVWGGQAVVGVVV
                     AEEDTDALLALRNRAFEQLVAVTLIAFTAAALVLLLFASRLSWRLRRLRDEAENAIDS
                     QGRVRALVGAEHARDEIGDLSRSFSTVLERLAQYNAYLEKLAGRLSHELRTPIAVVRS
                     SLDNLKLQSPSAETGVYLARAEEGIDRLETLLTRMAEATRLEQIVRSGERERFEAKTV
                     LAGCVEGYRAAYPQRRFELALIEEPVPLMGAPDLFAQMLDKLAANAADFAAPGSAIQI
                     SLARATREAVLRFVNTGPTLPAEMRATLFESMVSVRKERSGGAPHLGLGLYIVRLVAE
                     FHGGRVSATNRPDGNGVVFELRFPMAG"
     gene            complement(3277..3975)
                     /gene="pdsR"
                     /locus_tag="AC158Q_06960"
     CDS             complement(3277..3975)
                     /gene="pdsR"
                     /locus_tag="AC158Q_06960"
                     /inference="COORDINATES: protein motif:HMM:TIGR03787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteobacterial dedicated sortase system
                     response regulator"
                     /protein_id="HIR3339552.1"
                     /translation="MRRRIAIVEDDPVIRANYVDVLAKHGYEVAAYEGRREALAALRS
                     RLPDLVLLDIGLGDEVDGGFTLCRELRTLSGTLPIIFLTARDSDFDTVSGLRMGADDY
                     VTKNVSLPHLLARVSALFRRAELAREPAQGESILERGALRLDLDRFAVSWRGRGLELT
                     LTEFWLVHALARFPGHVKNREQLMGEAHVVVDDSTITSHVKRIRRKFGAVDPQFDSID
                     TVYGMGYRWKAEPS"
     gene            4158..4307
                     /locus_tag="AC158Q_06965"
     CDS             4158..4307
                     /locus_tag="AC158Q_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339553.1"
                     /translation="MQLQQKTTAFNARAVALEMTALMGYALLTGLAVSVLIALPVVWL
                     SATAL"
     gene            4438..>4651
                     /locus_tag="AC158Q_06970"
     CDS             4438..>4651
                     /locus_tag="AC158Q_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339554.1"
                     /translation="MILRLLAIGFIFFCTSIAWLVLAGVTSSRTHTADASLRSQVERV
                     WGAPQSQQQPGVTWTERVARRVESVED"
BASE COUNT          688 a         1616 c         1628 g          719 t
ORIGIN      
        1 aaccgcgagg atgcttcgcg ctcggcgctc agcactcgtc acttcgccgc cttgccggct
       61 ccggtgccgg cgatgcggcc gaacaccgcg ccggagacga gcccggtgcc gctcgggtag
      121 ttgaagtaga acaggccccc gaccatctcg cccgccgtgt agagccccgg gatcgggtgg
      181 aagtggacgt cgagcacctg gccggttgtc ttgtcgatgc gcagcccgcc gaaggtgaag
      241 gtgatgccgc aagtcgtggc gtaggcgtcg aacgggggcg tgtcgagcgg ctgcgcccag
      301 ttcgacttcg ctggcgagag ccctaccgtg catttgccat ccttgaccgt gtgatcgaac
      361 ggcacgtcct tcttcaccgc ggcgttgtaa tcgcgcacgg tcttcaggaa ctgctcggcg
      421 ttgacgccct ccagcttcgg ggcgagctcc tccagcgtgt tggcgctttc cttggtgacg
      481 aacttgatgc ggtactcgga gcgcagcagt ttcgtgacct tggaatcgaa cacctgccag
      541 gcgaactgcc ccggctgctt cagcacttcg ccgccgtact tggcgtaggt gaaggagtgg
      601 aaatccgcgc cctcgtccac gaagcgcctg ccctcggcgt tgatcagcag cccgaagatg
      661 tagctgtgct tctggaactg gtcgccgacg ttgacgtcgc cgaactccgg cgcgtagcgg
      721 tcccagccgg tggcgtggca gcccgaccag ttgccgcacg gcgcggcgcc gatgtcgagc
      781 gccatcctga ggccgtcgcc gacgttgaag cgcgagccgc gcaccttggc gagctcccag
      841 cccggcccga ggtagcgcgc gcgcatctcc gggttggcct cgaagccgcc gctggcgagg
      901 accaccgatt tcgcgcggaa ttcggccgct cttcccttgt gctgcgcgcg cacgccggtc
      961 acgcgctcgc cgtcgtacag gagcgacgcg gcgcgcgtct cgtagaacac ctcgatgccg
     1021 gctttctttg ccgccgcgtc caggtactgc accaggccgg cgccgccgcc gttcacttcg
     1081 atcggcatgc tgccgaagta cttgcgcttg gccccgacca tgcccgattg ccggccgtgg
     1141 ttcaggatga agcgcgcgcc cttcgagcgc agccacgcca tggtgtcgag gctgccgttg
     1201 accagcatct cggagaggtc gggatcggtg cggaagctcg tcagccggta gaggacgtcg
     1261 aggtattcct cccgggtgtt ggtgccgaaa tcgctgttgg cggcttcctc gtcgctgatg
     1321 tcggtgacgc gccgcaggtc ctcgagcgag ccgtaggcga agcgcatcac gccgccggcg
     1381 aagcggctgt tgccgccatg ctcttcctcg ctcgccgctt ccagcatggc gaccttcgcg
     1441 tcctgatccc tggccgacag cgccgcgcac agcgccgcgt tgcccttgcc gaccaccacc
     1501 acgtcgtatt cgaatttcac aggattgccg ggaccggtat ggaaaatgcg ggatcaagcc
     1561 aggagttggc cgcgaacgct gcgattctaa cccgccagcg agcgagtttc accgtgcctg
     1621 gcgcggcacg gacagcgcgc gcccagcatg tggacgagcg ttccggcgga atcggccggc
     1681 cgcggcggcg tcagccggcc atggggaagc gcagctcgaa cacgacgccg ttgccgtccg
     1741 gccggttcgt cgccgacacg cggccgccgt gaaattccgc gacgaggcgc acgatgtaga
     1801 ggccgagccc gaggtgcggc gcgccgccgg agcgctcctt gcgcaccgag accatggact
     1861 cgaacagcgt ggcgcgcatc tcggcgggaa gcgtcgggcc cgtgttgacg aaccgcagca
     1921 cggcctcgcg ggtcgcgcgc gcgagcgaaa tctggatcgc gctgccggga gcggcgaagt
     1981 ccgccgcgtt ggccgcgagc ttgtcgagca tctgggcgaa cagatcgggc gcgcccatga
     2041 gcggcaccgg ctcctcgatc agcgccagct cgaagcgccg ctgcggatac gccgcgcggt
     2101 agccctccac gcagcccgcc agcaccgtct tcgcctcgaa gcgctcgcgc tcgccgctgc
     2161 gcacgatctg ctccagccgc gtggcctcgg ccatgcgcgt cagcagcgtt tccagccggt
     2221 cgatcccttc ctcggcgcgg gccaggtaaa cgccggtttc cgcggacggc gactgcagct
     2281 tcaggttgtc gagcgaggag cgcaccacgg cgatcggcgt gcgcagctcg tgcgagagcc
     2341 gcccggcgag cttctcgaga taggcgttgt actgcgccag ccgctcgagc acggtggaga
     2401 agctgcgcga gagatcgccg atctcgtcgc gcgcgtgctc ggcgccgacc agcgcgcgca
     2461 cgcgtccctg cgagtcgatc gcgttctccg cctcgtcgcg cagccgccgc aggcgccacg
     2521 acaggcgcga ggcgaacagc agcagcacca gcgccgcggc cgtgaacgcg atcagcgtca
     2581 ccgcgaccag ctgctcgaag gcgcggttgc gcagcgccag cagggcgtcg gtgtcctctt
     2641 ccgccaccac cacgccgacc acggcctgcc cgccccagac cgggtgcgcg gcggagaaga
     2701 tgacgccgct gccggcgggg gcggggcgca tgcgccacgc cggagagccg tcgagcgcgc
     2761 gctcgacctc gcgcccgccg aggacgacgt cgttgggaat gaattcctcg gccggcggcg
     2821 ccggcgtgcc cagcacgcgc ttgaacacgg gctgcagcac ccggtgcacc aggcgctcga
     2881 gcgggccgaa ggtggcgtcg tcggccgcgg gcggcggcgg cgcatcgagg ctgcccgcga
     2941 tggcgacgag attgagcctg gcgtccagca cccagatgcg cacgcccgag cgggcgaggc
     3001 cggcgagcag caaccgcact tcttccatgg cggcgctggc ggccggcgaa ggcggtccct
     3061 ccggcgcggg cggcgccgcg cgcagttcca tcaggcccgg ccggtcctgc agggcagtgg
     3121 caactgcgcg cgcggtggcg acgatctgcc gctgctggtt ctcgcgcagc agccgctcca
     3181 ccgacttcac gtaggagtag cccacccacg ggatgacgag caggaccagc gcgaccagcg
     3241 cgatcttgga gcgcaggccg agggccgcgg acaggttcag cttggctccg ccttccaccg
     3301 gtagcccatg ccgtagacgg tatcgatcga atcgaactgc gggtccaccg cgccgaactt
     3361 gcggcggatg cgcttgacgt gggaggtgat ggtgctgtcg tccaccacca cgtgggcttc
     3421 gcccatcagc tgctcgcggt tcttcacgtg gcccgggaac ctggcgaggg catggaccag
     3481 ccagaactcg gtgagcgtga gctcgaggcc gcggccgcgc cagctgacgg cgaagcggtc
     3541 gagatcgagc ctgagcgccc cgcgctccag gatgctctcg ccctgggcgg gctcgcgcgc
     3601 gagctccgcg cggcggaaga gggcgctcac gcgcgcgagc aggtgcggca ggctcacgtt
     3661 cttggtgacg taatcgtcgg cgcccatgcg caggcccgac accgtgtcga aatcgctgtc
     3721 gcgcgcggtg aggaaaatga tcggcagcgt gcccgacagc gtgcgcagct cccggcacag
     3781 cgtgaagccg ccgtccacct cgtcgccgag cccgatatcg agcagcacca ggtccggcag
     3841 gcgcgagcgc agggcggcga gcgcctcgcg gcggccctcg taggcggcca cctcgtagcc
     3901 gtgttttgcc aggacgtcga cgtaattggc gcggatgacc gggtcgtctt caacgatggc
     3961 aatgcggcgg cgcatgcgga tttccgtggg gtgcgcttcg actataccgc atgggaatgc
     4021 gctcgcaaga ccgccgcggg aattgccata ttgtcgccac cattgaccgc cggatcgcca
     4081 ttttccggcc ccggcgcgcc catctttccg gtctccaata cgggtgtcgc cacattgcgg
     4141 cggcagaaag gatccggatg cagctccagc agaagacgac cgcgttcaac gcccgcgcgg
     4201 tggcgctcga gatgacggcg ctgatgggct acgccctgtt gacgggcttg gcggtctccg
     4261 tgctgatcgc cctgcccgtg gtgtggctgt cggcgaccgc cttgtaatgc cggtgccggg
     4321 gcgctgaggg ccggtggctg ggagtttctc cccacgtggc agcgcacaag gagcggtgcc
     4381 ggtttccagc cactggcccc cagcgcctcg ccagacaaat ataaacggga ggacacgatg
     4441 atcttgcgac tgctggccat cggcttcatc ttcttctgca cctcgatcgc ctggctcgtg
     4501 ctggcgggcg tcacgtcctc ccgcacccac accgcggacg cgagcctgcg ctcgcaggtc
     4561 gagcgcgtgt ggggcgcgcc gcagtcccag cagcagccgg gcgtcacgtg gaccgagcgg
     4621 gtcgcgcgcc gggtcgagag cgtcgaggac g
//
LOCUS       DBNGSG010000268         5119 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1104867, whole genome shotgun sequence.
ACCESSION   DBNGSG010000268 DBNGSG010000000
VERSION     DBNGSG010000268.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5119)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5119)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5119
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1104867"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="AC158Q_06975"
     CDS             <1..641
                     /locus_tag="AC158Q_06975"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3339555.1"
                     /translation="RRRESHFRWIKREIDAGRFGKLVQAEGNISRDRLGKIDLSSWRY
                     QAAGMPGGVMLQIGIHYTDVLEYLLGPIVAVRGQLAQLVLPGDNPDVANMILEHENGA
                     LSNVTASYASASEFYMMNIYGKEASAYYDLHQGLRFLKRGAERSEPVPFEKNDTIREE
                     LDEFADAARGKGRPEMDGEASTRSLAVLRAGIKSAREGRRVEVAEILNGGDI"
     gene            758..1288
                     /locus_tag="AC158Q_06980"
     CDS             758..1288
                     /locus_tag="AC158Q_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR3339556.1"
                     /translation="MSTEKKNASWPLDIYEMFRKLGIRQVAYVPDAGHIHLIKSCHAD
                     RNISTVSLTTEEEGVAMLAGSWLGGERGALLMQSSGAGNCINMLGIIQECRFPLLMLV
                     TMRGEWGEFNPWQLPMGQSTADILGRAGSVVQHVDDAARVGETVFAAGQMAFSTGRAV
                     AVLIGQRVVGFKDWSK"
     gene            1409..2005
                     /locus_tag="AC158Q_06985"
     CDS             1409..2005
                     /locus_tag="AC158Q_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010105480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR3339557.1"
                     /translation="MKKRIKSTLDRRVALGAILAKRGDALLVTGLGSTCYDAGTMDHP
                     QTFYLWGGMGAAAMLGLGLALAQPKRRVIVATGDGEMLMALGALATIGAQRPHNLAIV
                     VMDNELYSETGMQPTHTSRGVDLAGVAKAAGFARSGTVYNLKQLNAWVPALYKTPGPV
                     FLAAKVTSQRYPMSIRFRDGTAIKNRFREALLGSKAYD"
     gene            2157..3635
                     /locus_tag="AC158Q_06990"
     CDS             2157..3635
                     /locus_tag="AC158Q_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007749167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HIR3339558.1"
                     /translation="MAKYKMFIGGEWVEAATREHFESDNPFTGKPWALIPKGSAQDAD
                     RAVRAANKAFISGEWPKLNASKRGALLRKLGDLIGEKAKFLAEIEVRDNGKLLAEMSA
                     QTAYMAQWYYYYGGLADKIEGHVIPTDKPEMLNYTRYEPLGVVAAITPWNSPLLLTAW
                     KLAPALAAGNTVVLKPSEFTSASVLEFMKLIEQAGFPPGVVNVVTGFGPDVGTPLVEH
                     PLVAKVAFTGSDRTGTLIYQTAAKGLKRVSMELGGKSPNIVFDDAHIENAVKGVVSGI
                     FAATGQTCIAGSRLLVQQSIHDEFVGKLVAFAKTAKMGNPMSLDTQVGPVTNPPQFKK
                     ILDYIDIAKGEGAKPVLGGGKAARPECGDGWFVEPTIFTGVKNSMRIAQEEVFGPVLS
                     VIPFKDEEDAVAIGNDVVYGLAAGVWTQNMRRSLMMAERLQAGTVWINTYRAVSYLSP
                     FGGYKRSGLGRESGQEMIKDYMQVKSVWISTAAEVPNPFIMR"
     gene            4361..>5119
                     /locus_tag="AC158Q_06995"
     CDS             4361..>5119
                     /locus_tag="AC158Q_06995"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3339559.1"
                     /translation="MKQESIPTRSRSRELIEFVHGMKLEALPPDIVNRAKGCLLDTLG
                     CALFGSLQPWARIMAEEMLAEGSRGRSTLIGRKQTLAAPAAALCHGTASHGFELDDLL
                     DEAIVHPGAIIIPAALAAAESVDAPGSRLLLGIIAGYEILNRVGLAAGVEPAHRGFHK
                     TTVIGPVGAAVAAGVILELSAEQLVSATGLACSTSSGIKSFAAGTGGGMMKRMHAGRA
                     AESGVRMAQLAARDFTGPPTAIDGRFGLLEVYGGK"
BASE COUNT         1017 a         1614 c         1608 g          880 t
ORIGIN      
        1 agcggcgtcg cgaaagccat ttccgctgga tcaaacgcga gatcgacgcc ggccgcttcg
       61 gaaagctggt gcaggccgaa ggcaacattt cgcgtgatcg gctcggcaag atcgacctgt
      121 cctcgtggcg ctatcaggct gccggcatgc cgggcggcgt gatgctgcag atcggcattc
      181 actacaccga cgtgttggaa tacctgctgg ggccgatcgt ggctgtgcgc ggacaactcg
      241 cccagctcgt gctgccgggc gacaacccgg acgtcgccaa tatgatcctc gagcacgaga
      301 acggcgccct gtcgaacgtc accgcgtcct acgcctcggc gtccgagttc tacatgatga
      361 acatctacgg caaggaagcg agcgcatatt acgacctgca ccagggactg cgctttctca
      421 agcgcggagc cgagcgctca gagccggtgc cgttcgagaa aaacgacacg atacgcgaag
      481 agctggacga attcgcggac gccgcgcgcg ggaaaggcag gccggagatg gacggcgagg
      541 cctccacccg ctcgcttgcc gtgctgcgcg cggggatcaa gtcggcgcgc gagggacggc
      601 gcgtggaagt ggccgagatc ctgaacggcg gggacatctg agactcgagg ccggggtttc
      661 cggcagtatt ccgtcccgtc ataccactat cgttatccgt tcgttgcagt tatagccccc
      721 cctcgctagc attcagtctg gaaaccggag gaacccgttg agcacagaga aaaaaaacgc
      781 atcctggccg ctggacatct acgagatgtt caggaagctc ggcatccgcc aggtcgccta
      841 cgtgcccgat gccggccata tccacctcat caagagctgc cacgccgaca ggaacatctc
      901 gaccgtatcc ctcacgaccg aggaagaagg cgtcgccatg ctggccggca gctggctggg
      961 cggcgagcgc ggcgcgctgc tgatgcagtc ctccggcgcc ggcaactgca tcaacatgct
     1021 cgggatcatc caggaatgcc gcttcccgct gctgatgctg gtcaccatgc gcggtgaatg
     1081 gggcgagttc aacccctggc aattgccgat ggggcaaagc accgccgata tcctcggccg
     1141 cgccggctcg gtcgtgcagc acgtggacga cgctgcgcgc gtcggcgaga cggtgttcgc
     1201 ggccggccag atggcgttca gcacgggccg cgccgtcgcc gtgctgatcg gccagcgcgt
     1261 ggtcggcttc aaggactgga gcaagtgacc cccaccctat ccctccccct tgccaagggg
     1321 gagagatggg aggggattcg tgaatcgtga atcgaaaatt tttttcctgg cgccccttcg
     1381 acgtgctcag gacaagcttg gctcatatat gaaaaaacgc attaaatcga ctcttgaccg
     1441 ccgcgtggcg ctgggcgcca tcctcgcgaa gcgcggggat gccctgctgg tgaccggcct
     1501 gggctccacc tgctacgacg ccggcaccat ggaccatccg caaaccttct acctgtgggg
     1561 cggcatgggc gctgccgcga tgctgggcct ggggctcgcg ctggcgcagc cgaaacgcag
     1621 ggtgatcgtc gccaccggcg atggcgagat gctgatggcg ctgggggcgc ttgcgaccat
     1681 cggcgcgcaa cgcccgcaca accttgccat cgtggtgatg gacaacgaac tctatagcga
     1741 aaccggcatg caacccaccc acaccagccg cggcgtcgac ctggccggcg tggcaaaggc
     1801 cgccggtttc gcccgctccg gcaccgtcta taacctcaag cagttgaacg cctgggtgcc
     1861 ggcgctctac aagacccccg gcccggtttt ccttgcggcc aaggtgacgt cgcagcgcta
     1921 ccccatgtcg atccgcttcc gggacggcac cgcgatcaag aaccgtttcc gcgaggcctt
     1981 gctgggcagc aaggcctatg actagaagtg actcgttgct cgtttctcgt tcctcgttca
     2041 gtacacgcct tgcggacttc gcgcagcaaa gcacgagaaa cgagtaacga gttacgaatc
     2101 actttttaga aacaagcgcg agcgtaaaga cccatatgga tacccctacc agtacgttgg
     2161 ccaagtacaa aatgttcatc ggcggcgagt gggttgaagc cgccaccagg gagcatttcg
     2221 agagcgacaa cccgttcacc ggcaagccat gggcgttgat tccgaaaggc agcgcgcagg
     2281 acgccgaccg cgcagtgcgc gcggcgaaca aggcgttcat ctcgggtgag tggccgaagc
     2341 tcaatgcctc gaagcgcggc gcgctgctga ggaagctcgg cgacctcatc ggcgagaaag
     2401 cgaagttcct tgccgagatc gaggtgcgcg acaacggcaa gctcctcgcc gaaatgagcg
     2461 cacagaccgc ctacatggcg cagtggtatt actactacgg cggcctcgcg gacaagatcg
     2521 aagggcatgt catccccacc gacaagcccg agatgctgaa ctacacgcgc tacgaaccgc
     2581 tcggggtcgt cgccgctatc acgccgtgga actctcccct gctgctcacc gcgtggaagc
     2641 ttgcgccggc gctcgcagcc ggcaacacgg tggtgctcaa gccctccgag ttcacctcgg
     2701 cttccgtgct ggagttcatg aagctgatcg agcaggccgg attcccgccc ggcgtggtca
     2761 acgtcgtgac cggtttcggg cccgacgtcg gcacaccgct ggtggaacat cctctcgtgg
     2821 cgaaagtcgc gttcaccggc tcggaccgga ccggcacgct gatctaccag accgcggcca
     2881 agggcctgaa gcgcgtcagc atggagctcg ggggcaagtc gcccaacatc gtgttcgacg
     2941 acgcgcacat cgagaacgcg gtgaagggcg tggtatccgg gattttcgca gcgaccggcc
     3001 agacctgcat cgccggctcg cgcctgctgg tacagcaaag tatccacgac gagttcgttg
     3061 ggaagctggt cgcgttcgcg aaaaccgcga aaatgggcaa tccaatgagc ctcgacacgc
     3121 aggtcgggcc ggtcaccaac ccgccgcaat tcaagaaaat cctcgattac atcgacatcg
     3181 cgaagggcga aggcgcgaaa ccggtgctgg gcggcggcaa ggccgcgcgc cccgagtgcg
     3241 gcgacggctg gttcgtcgag cccaccatct tcaccggcgt gaagaactcg atgcgcatcg
     3301 cgcaggagga agtgttcggc ccggtcctgt cagtcatccc gttcaaggac gaggaagacg
     3361 cggtcgcgat cggcaacgac gtggtctacg ggcttgccgc cggcgtgtgg acgcagaaca
     3421 tgcgccgttc gctcatgatg gccgagcgcc tccaggccgg cacggtgtgg atcaacacct
     3481 accgcgcggt gtcctacctc tcccccttcg gcggctacaa gaggagcggc ctcgggcgcg
     3541 agagcggtca ggagatgatc aaggactaca tgcaggtgaa gagcgtgtgg atctcgacgg
     3601 cggcggaagt gccgaatcct ttcatcatgc gctaaaacct gaatcgaacc gccaagacgc
     3661 caagagaaac aaactatact gaaccgccaa ggccgccaag cacgccaaga aagacagatc
     3721 attatggtgt caactaattt gacatctaac aagtcaacca attcgtcaac taattgaagt
     3781 tagacagcgc acatgcccta tcgcgctctt actcttgttg ccgcattgag cacgccaccg
     3841 gtctgggcga gtttgatttc tgtcccgact caggaacaat gtttgaagca gagcggcatt
     3901 tggtcgaagg atgaccggct ggacgcacca ggtcactatt gcctctttcc agagaagaaa
     3961 gggaactgtg agtcaggtgg cggagaatgg gtaccgtttg gaggggtaac tttcacgtgt
     4021 cggcacaaga gtcgagacgg aggcaaaccg tgcaaagact catcagaatg ccagtttcgt
     4081 tgcatacctg aaaaagacaa gacaagaagc ggcacgtgtg cacccgatga caacccgttc
     4141 ggatgttcgc tggtcaagag tggcgccgtc atatgcgttc gataacgctg tctaaccccg
     4201 cgctcaacac ggacgtggtg cgtccgcagc gcgccggtta gcgcgaacgt taaattttga
     4261 ttatggattc tcttggcgtg cttggcggtc ttggcggttc gtatttattt ttgattgatt
     4321 ttctcggcgg ccttggcgtc ttggcggtta atttaatgct atgaaacaag aaagcatccc
     4381 cacgcgcagc cgctcgcgcg agctgatcga gttcgtgcac gggatgaagc tggaagcgct
     4441 gccgccggat atcgtcaacc gcgccaaggg ctgcctgctc gacacgctcg gctgcgccct
     4501 cttcggcagc ttgcaaccgt gggcgagaat catggccgag gagatgctcg cggaaggctc
     4561 gcgtggccgc agcaccctca tcggccggaa gcagacgctg gcggcaccgg ccgcggcgct
     4621 gtgccatggc accgcctcgc atggcttcga actcgacgac ctgctcgacg aggcaatcgt
     4681 ccaccccggg gccatcatca tcccggccgc gctggccgcc gccgaatccg tggacgcgcc
     4741 gggctcccgg ctgctgctcg gcatcatcgc cggctacgag atcctcaacc gcgtcggcct
     4801 cgccgccggc gtcgagcccg cgcaccgggg ctttcacaag acgaccgtca tcggcccggt
     4861 cggcgccgcc gttgctgccg gcgtgatcct tgagctgagc gccgagcaac tcgtgtccgc
     4921 gaccggcctc gcctgctcca cctcctccgg catcaagagc ttcgctgcgg gcaccggcgg
     4981 cggcatgatg aaacggatgc acgccggtcg cgccgcggaa tcgggcgtgc gcatggcgca
     5041 gctcgccgcg cgcgacttca cagggccgcc caccgcgatc gacgggcgct tcgggctgct
     5101 ggaggtctac ggcggaaag
//
LOCUS       DBNGSG010000269         1615 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1783977, whole genome shotgun sequence.
ACCESSION   DBNGSG010000269 DBNGSG010000000
VERSION     DBNGSG010000269.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1783977"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="AC158Q_07000"
     CDS             <1..255
                     /locus_tag="AC158Q_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339560.1"
                     /translation="RSCGAAIAEKAIVCYRCGAPTAAEPAPVRPASRAPALWVTLLLV
                     AAIAVAGWMAAQTSVVLTLVIWIMVAVLLAVPVLVRRRRR"
     gene            272..>1615
                     /locus_tag="AC158Q_07005"
     CDS             272..>1615
                     /locus_tag="AC158Q_07005"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR3339561.1"
                     /translation="MYTDTLEMGEPRIEVRDVLGERTVRVDRMPFTIGRRETNDLRLG
                     GSEVSREHAEIVNTSGRYLLRDKESRYGTFVNGELVTECELRSGDKVRLGRGGGADLV
                     FLSAEDSRTGSRSTTGARDDLRQITALLEGFRALGSGRVLQDVLALVLDAALEISGAE
                     RGFIMLSTAEGQLEFRLARGRDKQTLTDSTFAVSRKIPEEVYLSGKTKVVADLLDGGL
                     ADVHVGTVALGIRNVVCIPLNWLRLVESIEARGEDRRIGVLYLDSRERGALLSDPTRS
                     ALETLAAEASVAIENAQLYRANLEKARMEQEMRIAAEIQQALLPKPRASLGFVEAAAA
                     SIPCRSIGGDFFDYLDEPGRAFGFTLGDVAGKGPPAALMSALMQGMFASQALYADSPS
                     VAVTQMNKALCRRGLESRFVTLMFGIMDSDGALAYCNAGHNPPLVIGTSGIRRLEA"
BASE COUNT          228 a          535 c          587 g          265 t
ORIGIN      
        1 cgatcttgcg gcgcggccat cgccgagaag gccattgtct gttaccggtg cggcgcgccg
       61 acggctgccg agccggcacc ggtgcggccg gcgtcccgcg cgcccgcgct gtgggtgacg
      121 ctgctgctgg tcgccgccat cgccgtcgcg gggtggatgg cggcacaaac ttccgtggtg
      181 ctcacgcttg taatttggat catggtggcc gtgctgctgg cggtgccggt cctggtccgc
      241 cggcggcggc ggtagcgccg gcggagcgtt cgtgtacact gacacgcttg agatgggtga
      301 accgaggata gaggtccggg acgtcctggg cgagcgcacg gtccgggtcg accggatgcc
      361 gtttaccatc ggacgccggg agaccaacga ccttcgcctg ggcggcagcg aagtgtcccg
      421 tgagcacgcc gaaatcgtca acacttccgg gcgctacctc ctccgcgaca aggaatcccg
      481 ctacgggacg ttcgtgaacg gggagctcgt caccgagtgc gagctccgaa gcggcgataa
      541 agtgcgcctc ggccggggcg gtggcgccga tctcgtcttt ctgtcggccg aggactcgcg
      601 cacgggttcg cgttccacga ccggcgcgcg cgatgacctg cgccaaatca cggccctgct
      661 cgagggcttt cgggcgctcg gctcgggccg cgtgctgcag gacgtgctgg cgctcgtcct
      721 cgacgccgcg ctcgagattt ccggggcgga gcgcgggttc atcatgctct ccacggccga
      781 ggggcagctc gagttccggc tggcgcgcgg ccgcgacaag cagacgctga ccgacagcac
      841 ctttgccgtc agccggaaga tccccgaaga ggtctacctg tcgggcaaga ccaaggtcgt
      901 cgccgacctc ctcgacggcg gactggccga cgtccacgtc ggcacggtcg cgctcggcat
      961 ccgcaacgtg gtctgcatcc ccttgaactg gttgcggctg gtggagtcca tcgaagcgcg
     1021 gggcgaggat cggcgcatcg gcgtgctcta cctcgacagc cgggagcgcg gagcgctcct
     1081 gtcggatccc acgcggtcgg cgctcgagac gctggcggcg gaggcgtcgg tcgccatcga
     1141 gaacgcgcag ctctaccgcg cgaacctcga aaaggcgcgc atggagcagg agatgcgcat
     1201 cgcggctgag attcagcagg cgctgctgcc gaagccgcgc gcgagcctcg ggttcgtgga
     1261 ggcggccgcc gcgagcattc cgtgccggtc gatcggaggc gattttttcg attacctcga
     1321 cgagccgggc cgggcgttcg gcttcacgct gggcgatgtg gccggcaagg gtcccccggc
     1381 cgcgctcatg agcgcgctga tgcagggcat gttcgcgtcg caggcgctct acgccgacag
     1441 cccgtcggtc gcggtgaccc agatgaacaa ggcgctgtgc cgccgcggcc tggagtcgcg
     1501 gttcgtgacg ctgatgttcg gcatcatgga ctccgatggc gcgctggcgt actgcaacgc
     1561 cggccacaat ccgccgctcg tgatcggcac ctccggtatt cgtcgcctgg aggcc
//
LOCUS       DBNGSG010000270         7450 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2971913, whole genome shotgun sequence.
ACCESSION   DBNGSG010000270 DBNGSG010000000
VERSION     DBNGSG010000270.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7450)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7450)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7450
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2971913"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /gene="ispH"
                     /locus_tag="AC158Q_07010"
                     /gene_synonym="lytB"
     CDS             complement(<1..813)
                     /gene="ispH"
                     /locus_tag="AC158Q_07010"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HIR3339562.1"
                     /translation="MQVLLANPRGFCAGVDRAIEIVERALTIHGAPIYVRHEVVHNKF
                     VVQDLRAKGAVFVEDLAEVPEGATVIFSAHGVSQAVRREAQARRLKVFDATCPLVTKV
                     HVEVDKMRAAGREIVMIGHRGHPEVEGTMGQSAGGMYLVEKPGDVAQLKVRDENHLAF
                     VTQTTLSVDDAQHTINALKARFPAIVGPKKDDICYATQNRQDAVKALAKRCDVVIVVG
                     SPNSSNSTRLREVAEHQGVAAYMVDNALELDPEWVAGKGVIGITAGASAPEVL"
     gene            complement(920..1438)
                     /gene="lspA"
                     /locus_tag="AC158Q_07015"
     CDS             complement(920..1438)
                     /gene="lspA"
                     /locus_tag="AC158Q_07015"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HIR3339563.1"
                     /translation="MMKGYALMRRWLALAALIVVADQAAKYAAIQYFAANQAVAVTPF
                     LNLVLVYNPGAAFSFLSDAAGWQRGLFIAIALIASAWIIYLLRQYAHQRLFALALALV
                     LAGAVGNVIDRIYIGAVVDFIDFHAYGWHWPAFNVADSAITCGAVLLVWDALRPTKDP
                     IPEPPINADKRR"
     gene            complement(1435..4245)
                     /gene="ileS"
                     /locus_tag="AC158Q_07020"
     CDS             complement(1435..4245)
                     /gene="ileS"
                     /locus_tag="AC158Q_07020"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HIR3339564.1"
                     /translation="MADYKKTLNLPDTPFPMRGDLAKREPLMLKAWQERRFYERIRKA
                     RRGRERFVLHDGPPYANGDIHICHAVNKILKDIIVKSKSLSGFDSPYVPGWDCHGMPI
                     EVQIEKIHGKNLPAKETQKLARAYAAEQVERQKRDFIRLGVLGDWDNPYLTMACRNEA
                     DEIRALGVLLEKGYVYRGLKPVNWCFDCGSALAEAEVEYEDRKDIAIDVGFPFADPEK
                     IAKAFGLARLPDQPGYAVIWTTTPWTLPGNQALNVHPEYSYNLVQTERGLIILAAELT
                     TACLDRYDLKQRDVLGTCKGAALEHIHFRHPFYDRLAPVYLGDYVTLDTGTGIVHSAP
                     AYGVEDFISCRRYGMKDDEILTPVMGDGKYAPALPLFGGEMIWKANPKIVTALGERGM
                     LFAKADHVHSYMHCWRHKTPVILRATVQWFAGMDEVPGYHGVKPSESLRDTALRGVEK
                     TQFYPAWGKARLHGMIANRPDWTLSRQRQWGVPMPFFIHKETGRLHPRSLDLLEQVAK
                     RVERGGIEAWQSLEAEELLGDDAASYEKVRDTLDVWFDSGSTHRTVLRGSHQTDGTFP
                     ADLYLEGSDQHRGWFHSSLLVASMMDGEAPYKALLTHGFVIDLEGRKMSKSKGNAMSP
                     QNIVDTLGADILRLWVASSDYSGELTISSEILKRVVESYRRIRNTLRFLLANTADFNL
                     ARHRLPAAEWVEIDRFALELAHGLQKELTGHYERYEFHLAMQKFVKFCSEDLGGIYLD
                     ILKDRLYTTAEDSKSRRSAQNALWHITNSLLRLMSPVLSFTAEEAWEVFSGNPERCIF
                     EEEWYALPLSRLDQSRIDGWWDVRNTREIANKKIEEKRAQGVIGSSLQAELDIYASGP
                     ILESLNRLGDDLNFVFLTSRATVHHREAAGPGVEVTSSPHRKCERCWHYRADMGADAA
                     HPEICGRCVSNVSGNGELRTHA"
     gene            complement(4280..5233)
                     /locus_tag="AC158Q_07025"
     CDS             complement(4280..5233)
                     /locus_tag="AC158Q_07025"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HIR3339565.1"
                     /translation="MRVTRGVPAAAAGPAALTIGNFDGVHLGHQAMLAELKRAAGRLG
                     LPACVLTFEPHPREFFAPDKAPTRLTSLREKLELLAGCGVDRVHICRFNYGLAQTTAE
                     EFVERIIARGLMARWVLVGDDFRFGARRAGSLVMLKQAGPQLGFEVAAIASVMLDGER
                     VSSTALRHALVAGDLDRAGRLLGRGYSISGRVVAGDGLGRKLGYPTANVQMKRNRPPL
                     SGIFTVKMHGAAERPVNGVASLGVRPTVKQQGAPVLEVHVLDFKGDLYRRHVRVEFLR
                     KLRDEEKYADLATLTRQIALDVEHAKSFFSRQDAKTPRKTG"
     gene            complement(5310..6086)
                     /gene="cobA"
                     /locus_tag="AC158Q_07030"
     CDS             complement(5310..6086)
                     /gene="cobA"
                     /locus_tag="AC158Q_07030"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HIR3339566.1"
                     /translation="MKRAMKQAAGRVFLVGAGPGAPDLLTLRAAEVLKRADVVFHDAL
                     VHPEVLALAGHAEKIAVGKRCGRFATAQQFINKRLADAAWKHNVVVRLKGGDPMLFGR
                     AQEEISALEAAGVPYEVVPGVTSALAAAAEAGVSLTQRGVARSVTFVTPRVGQGEAPS
                     AWAKSAVAADTAIFYMGAGQAQEITTALLAAGAAPDLPVLVAENATLPQARRIALTLE
                     ELPEIARYGLTGPTLIMVGRAFAAALAAAEQEALSKYARA"
     gene            complement(6091..6624)
                     /locus_tag="AC158Q_07035"
     CDS             complement(6091..6624)
                     /locus_tag="AC158Q_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017423669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HIR3339567.1"
                     /translation="MALIIKDRNVVTDPWLRLELGADASFPPVPPAGDIIVPLAVWQR
                     QRVHLIVRPGRLGVWLDSHEDPGVIAEDLKLFGVVAVNFPKFGDGRGYSTARLLRERY
                     GYRGELRAIGDVIRDVLFQLSSCGFNAFALRQGEDPQEALAGFRDFSESYQATIDQPL
                     PLFRRRFPVSTDGAERS"
     gene            complement(6569..>7450)
                     /locus_tag="AC158Q_07040"
     CDS             complement(6569..>7450)
                     /locus_tag="AC158Q_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008112348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HIR3339568.1"
                     /translation="ATLTEEEVRRVEGRFTRPRYETLPGDDAGHLARMASEPAYAAWT
                     KRNVHPHKAPGYAAVTLSLKKTGVPPGDATDGQMDAVADLADRYSQGELRVSHEQNLI
                     LADVRQSDLHALWQQARALGLATPNIGLITNIIACPGGNFCSLANAKSIPVAQAIQER
                     FDRLDYVYNIGELDLNISGCMNSCGHHHVGHIGILGVDKNGEEWYQISIGGNQGLTRP
                     GAPAAVGKVIGPSFARDDIPDVIEKLVREYLDRRESEEERFIDVVWRAGIEPFKERVY
                     GTHHQRQKRRNRSLVAA"
BASE COUNT         1278 a         2439 c         2380 g         1353 t
ORIGIN      
        1 cagtacttcc ggcgctgaag cgccggccgt aatgccgata acacccttgc cggccaccca
       61 ctctggatca agctccagcg cgttatccac catataggcc gcgaccccct ggtgctcggc
      121 cacttcgcgc agccgggtcg agttggagct gttgggcgag cccaccacga tcaccacgtc
      181 gcagcgcttc gccagcgcct tcacggcgtc ctggcggttt tgcgttgcgt agcagatgtc
      241 gtccttcttc ggtccgacga tggcgggaaa gcgcgctttc agggcattga tcgtgtgctg
      301 cgcgtcgtcc accgacagcg tggtctgagt cacgaaggcg agatggttct cgtcccggac
      361 cttgagctgc gcgacatctc ccggcttctc cacgaggtac atgccgccgg cgctctgccc
      421 catggtgccc tcgacctcgg gatggccgcg gtggccgatc atgacgatct cgcgcccggc
      481 cgcgcgcatc ttgtcgacct cgacgtgcac tttggtcacc agcggacaag tggcgtcgaa
      541 cactttcagc cggcgcgctt gcgcctcgcg cctcactgcc tgcgacacgc cgtgggcgct
      601 gaaaatgacc gtggcgccct cgggcacctc ggcgaggtcc tcgacgaaga ccgcgccctt
      661 ggcccggagg tcctgcacca cgaacttgtt gtgcaccact tcgtggcgca cgtagatcgg
      721 cgcgccgtgg atggtgagag cgcgctcgac gatctcgatc gcgcgatcga cgccggcgca
      781 gaaaccgcgg ggattggcta gcaatacttg cattacgaag aattataaat cgatcaaatc
      841 agccgccgat ggacgctgat ggacgcggat aagatcaaaa ctccaactag acaggatata
      901 tgccgtccat ttccctattt catcggcgtt tatcggcgtt tatcggcggc tcaggaatcg
      961 ggtctttagt tggacgcaag gcgtcccaga cgagcaggac cgcgccgcag gtaatggccg
     1021 aatcggcgac attgaacgcg ggccagtgcc atccgtaagc gtggaaatcg ataaaatcca
     1081 ccaccgcccc gatgtagatg cggtcgatga cgttgcccac cgcgccggcc agcaccagcg
     1141 ccagcgccag cgcaaacagg cgctggtgcg cgtactggcg cagcagatag atgatccagg
     1201 ccgaagcgat gagcgcgatg gcgataaaca ggccgcgctg ccagccggcc gcgtccgaaa
     1261 gaaagctgaa cgcggccccg gggttataga ccaggaccag gttgagaaaa ggcgtgaccg
     1321 cgacggcctg gttcgcggcg aaatactgga tcgcggcgta tttggcggcc tggtccgcga
     1381 cgacgatcag cgccgccaat gctagccacc tacgcatgag tgcgtagccc ttcatcatgc
     1441 atgcgtgcga agctcgccat ttccggaaac gttggagacg cagcgcccgc agatttccgg
     1501 atgcgctgca tccgcgccca tgtcggcacg gtaatgccag caacgctcgc attttctgtg
     1561 agggcttgac gtaacctcca cgcccggccc cgctgcctcc cggtgatgca cggtcgctcg
     1621 cgaagtgagg aaaacaaagt tcaagtcatc tcccaggcgg ttcagggact cgagtatcgg
     1681 cccggacgcg tagatatcca gttcggcctg cagggacgaa ccgataacgc cctgtgcccg
     1741 tttctcctcg attttcttgt tcgcgatctc cctcgtgttt cggacatccc accagccgtc
     1801 gatccgggat tgatccaggc gcgacaacgg aagcgcgtac cattcttcct cgaagatgca
     1861 gcgctcgggg ttcccgctga acacttccca ggcttcttcg gcggtgaagc tgagcacggg
     1921 cgacatcaat cgaagcaggc tgttcgtgat gtgccacaac gcgttctgcg cggaccggcg
     1981 ggacttggaa tcttccgccg tcgtgtacaa ccggtccttg aggatgtcga gatagatccc
     2041 gcccaggtcc tccgagcaga atttgacgaa tttctgcatc gccaggtgaa attcgtaacg
     2101 ttcgtagtgg cccgtcaact ccttttgcag gccgtgcgcc agttccagcg caaagcgatc
     2161 gatctcgacc cactcggccg ccggcagccg gtggcgggcc aggttgaaat cagcggtgtt
     2221 ggcgagcaga aaccgcagag tattgcggat gcggcggtag gactcgacca cgcgtttcag
     2281 aatctcgctt gaaatggtga gctcgcccga ataatcgctg ctggcgaccc atagcctcag
     2341 gatatccgcg ccgagcgtgt ccacgatgtt ctgcggggac atcgcgttgc ccttcgactt
     2401 cgacatcttg cggccttcga ggtcgatgac gaagccatgg gtcagcagcg ccttgtatgg
     2461 cgcctcgccg tccatcatgg aagcgaccag cagcgaggaa tggaaccatc cgcggtgctg
     2521 gtccgagccc tcgaggtaca ggtccgccgg gaatgtgcca tcggtctggt gagagccgcg
     2581 cagcacggtg cggtgcgtcg agccggaatc gaaccacacg tcgagcgtgt ccctcacctt
     2641 ctcgtaactt gccgcgtcgt ccccgagcag ctcttccgcc tcgagcgact gccaggcctc
     2701 gatgccgccg cgctcgacgc gtttcgcgac ctgctccagc aggtcgagcg aacgcggatg
     2761 gagccggccg gtttccttgt ggatgaaaaa cggcatcggc actccccact ggcgctggcg
     2821 cgacagcgtc cagtcggggc ggttcgcgat cattccgtgg aggcgcgcct tgccccatgc
     2881 cgggtagaac tgcgtctttt cgaccccgcg cagtgccgta tcgcgcagcg attcggacgg
     2941 cttcacgccg tggtagccgg gcacttcatc catgccggcg aaccactgca cggtggcgcg
     3001 caggatgacg ggtgtcttgt gccgccagca gtgcatgtag ctatgaacgt ggtccgcctt
     3061 cgcgaacagc atcccgcgct ccccgagcgc tgtcacgatc ttcgggttgg ccttccagat
     3121 catctcgccg ccgaagagcg gcagcgcggg ggcatatttg ccgtcaccca tgaccggcgt
     3181 gaggatctcg tcgtccttca ttccgtagcg gcggcacgag atgaagtctt ccacgccgta
     3241 ggccggcgcc gaatgcacga tgccggttcc ggtatcgagc gtcacatagt caccgaggta
     3301 aaccggagcc agccggtcgt agaacgggtg tcggaagtgg atgtgctcga gcgccgcgcc
     3361 cttgcaggtt ccgagaacgt cgcgctgctt gaggtcataa cgatcgagac aggccgtcgt
     3421 cagctcggca gcaagaatta tcaatccacg ctcggtctgc acgaggttgt acgagtactc
     3481 ggggtgcaca ttgagcgcct ggttgcctgg tagcgtccac ggcgtcgtgg tccagatcac
     3541 cgcgtagccc ggctggtcgg gaagcctggc caggccgaac gctttcgcga tcttttccgg
     3601 atcggcgaac gggaaaccga cgtcgatggc gatgtccttg cgatcctcgt attcgacctc
     3661 ggcctcggcc agggccgatc cgcagtcgaa gcaccagttg acgggcttga gtccgcggta
     3721 gacgtaaccc ttctccagca gcacgccgag cgcccggatt tcgtcggcct cgttccggca
     3781 ggccatggtg aggtacggat tgtcccagtc gcccaggacg ccgagacgga tgaaatcccg
     3841 tttctgccgc tcgacctgct cggcggcata ggcgcgggcg agcttctgcg tttccttcgc
     3901 cggcaggttc ttgccgtgga tcttctcgat ctgcacctcg atcggcatgc cgtggcagtc
     3961 ccagccgggc acatacggcg agtcgaaccc ggacagggat ttgctcttga cgatgatgtc
     4021 cttgagtatc ttgttgacgg cgtgacagat gtggatgtct ccgttcgcgt acggcgggcc
     4081 atcgtggagc acgaaacgct cgcggcctcg gcgggccttg cggatgcgtt cgtagaacct
     4141 gcgctcctgc caggccttca gcatcagcgg ctcgcgcttc gcgaggtcgc cgcgcatcgg
     4201 gaacggggta tccggcaggt tgagcgtttt cttgtaatca gccatgagca gcggtcagcg
     4261 gtcagctatc agcatgttgt taacccgtct tccttggcgt cttggcgtct tggcggctga
     4321 aaaagctctt cgcgtgctct acgtcgagcg cgatctggcg cgtcagggtc gcgagatcgg
     4381 catacttctc ctcgtcgcgc agcttgcgca gaaattccac gcgcacgtgg cgccggtaga
     4441 gatcgccctt gaagtcgagc acatggacct ccagcaccgg ggctccctgc tgctttaccg
     4501 tcgggcgcac gccgaggctg gccacgccgt tcaccgggcg ctccgccgcg ccatgcatct
     4561 ttaccgtgaa aatgccgctg agcggaggac gattgcgctt catttgcacg ttggcggtcg
     4621 ggtagccgag cttgcgtccc agcccgtcgc cggcgaccac gcgcccgctg atcgagtagc
     4681 cgcgacccag caaacggccc gcgcggtcga ggtctcccgc aacgagcgcg tggcgcagcg
     4741 cggtgctcga aacgcgctcg ccatccagca tcacgctggc gatcgcggcg acctcgaagc
     4801 cgagttgggg gcccgcctgc ttcagcatca cgaggctgcc cgcgcgccgc gcgccgaaac
     4861 gaaagtcatc gcccaccagc acccagcgcg ccatcaggcc ccgggcgatg atgcgctcga
     4921 cgaattcctc cgcggtcgtc tgggccagcc cgtagttgaa gcggcagatg tgaacgcggt
     4981 ccacgccgca gccggcgagc aactccagct tctcgcgcag cgaagtgagc cgcgtcggcg
     5041 ccttgtcggg ggcgaagaat tcgcgcgggt gcggctcgaa ggtcagcacg catgcgggca
     5101 ggccgaggcg gccggccgcg cgcttcagtt cggccagcat cgcctggtgg ccgagatgaa
     5161 cgccgtcgaa attgccgatg gtgagcgcag ccgggccggc tgcggcggcg ggaacgccgc
     5221 gagtaacgcg catacgaccc agctttaagg cgaagcctaa aagcttgaat tgtagcgtgt
     5281 tttaagagat acggtccagc ttctcgagct cacgcgcggg cgtacttgct cagtgcttcc
     5341 tgttcggccg cagcgagcgc cgcagcgaat gcgcgcccga ccatgatgag cgtcgggccg
     5401 gtgagtccgt agcgcgcgat ctccggcagt tcctccagcg tcagcgctat gcgccgggcc
     5461 tgcggcaacg ttgcgttctc cgcgaccagc acgggaagat caggcgcggc gccggcggca
     5521 agcaaggcgg tcgtgatttc ctgcgcctgt cccgcgccca tgtagaagat cgccgtgtcc
     5581 gcggcaacgg cgctcttcgc ccacgcactt ggggcctcgc cctgccccac cctgggagtg
     5641 acgaaggtga cgctgcgcgc aacaccgcgc tgtgtcagcg acacgcccgc ctcggcggca
     5701 gccgccagcg cggaggtcac gccggggaca acctcatacg gcacgccggc cgcctcgagc
     5761 gcgctaatct cttcctgggc gcgcccgaac agcatgggat cgccgccctt gaggcggaca
     5821 accacgttgt gtttccatgc cgcatccgcg agccgcttgt tgatgaactg ctgcgccgtg
     5881 gcgaagcggc cgcagcgctt gccgaccgcg attttctcgg cgtggccagc cagggcaagc
     5941 acttccggat gcaccagcgc gtcgtggaac acgacatcgg cccgcttcag gacctccgct
     6001 gcgcgcaacg taagcaggtc cggcgcgccg ggaccggcgc ccacgagaaa taccctgccc
     6061 gccgcctgtt tcatggcccg cttcatgtcg tcacgaccgt tccgcgccat cggttgaaac
     6121 cgggaagcgg cggcgaaaca acggcagcgg ctgatcgatc gtggcctggt agctctcgct
     6181 gaaatccctg aacccggcca gcgcctcctg cgggtcttcg ccttgacgca gcgcgaaggc
     6241 attgaagccg caactggaca gctggaagag cacgtcgcga atcacgtcgc cgatcgcgcg
     6301 cagctcaccc cggtaaccat agcgttcgcg cagcagccgc gcagtcgaat agccgcgccc
     6361 atcgccgaat ttcgggaaat tcaccgccac gacgccgaag agcttcaggt cttcggcaat
     6421 gacgcccgga tcctcgtggc tgtccagcca caccccgagc cggccgggac ggacgatcag
     6481 atgcacgcgc tgccgctgcc acacggcgag cggcacgatg atgtctcccg cgggcggtac
     6541 cgggggaaaa ctggcgtcgg cgcccagttc aagccgcaac cagggatcgg ttacgacgtt
     6601 tctgtctttg atgatgagtg ccataaacac gttctttgaa gggttcgatt ccggcgcgcc
     6661 ataccacgtc gatgaagcgc tcttcctcgc tctcgcgccg gtcgaggtat tccctgacca
     6721 gcttctcgat cacgtcgggg atatcgtcgc gggcgaacga cgggccgatg accttcccga
     6781 ccgccgcggg cgcgccgggg cgcgtgagcc cctggttgcc gccgatggaa atctggtacc
     6841 actcctcgcc attcttgtcg acgccgagga tgccgatgtg gccgacgtgg tgatggccgc
     6901 aggaattcat gcagccggag atgttgagat cgagttcgcc gatgttgtac acgtaatcca
     6961 gccggtcgaa gcgctcctgg atcgcctgcg cgaccggaat ggacttggcg ttggcaagcg
     7021 agcagaaatt gccgccgggg caggcgatga tgttggtgat gagtccgatg ttgggcgtgg
     7081 cgagccccag cgcccgcgcc tgctgccaca gggcgtgcag gtccgactgg cgcacgtcgg
     7141 cgaggatcag gttctgctcg tgggacaccc gcagctcgcc ctggctgtag cggtcggcga
     7201 ggtccgccac cgcgtccatc tgcccatcgg tggcgtcgcc cggcggcacg ccggttttct
     7261 tcagcgacag cgtcaccgcg gcgtagcccg gcgccttgtg cgggtggacg ttgcgcttcg
     7321 tccaagcggc ataggcgggc tccgatgcca tcctcgcgag atgcccggca tcgtcgcccg
     7381 gcagcgtctc ataccgcggg cgggtaaagc gcccctccac gcgccgcacc tcttcctcgg
     7441 tcagggtggc
//
LOCUS       DBNGSG010000271         3750 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4671524, whole genome shotgun sequence.
ACCESSION   DBNGSG010000271 DBNGSG010000000
VERSION     DBNGSG010000271.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4671524"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..404
                     /locus_tag="AC158Q_07045"
     CDS             27..404
                     /locus_tag="AC158Q_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339569.1"
                     /translation="MRALSLPVMVLAGCATLADVDKARDSWHGARFDEVVSRWGPPNR
                     SATLADGREISTWVAEGVSGGLPVAIGVFGGSGGGGIGASIGLPGGGELQRCERTLTF
                     RNDRVVEQTWLGHPALCSSFKKE"
     gene            complement(504..1424)
                     /locus_tag="AC158Q_07050"
     CDS             complement(504..1424)
                     /locus_tag="AC158Q_07050"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3339570.1"
                     /translation="MLAAGVASAQQYPVKPVRIITGFSGGSELMARMVAQQLTPALGQ
                     QVLVDPRLGAAGSIGFEAAARSAPDGYTLLMGAVPLLTNPFIQPRVGFDPMRDFAPIV
                     LLSTIPNGLFLHPSVPAKSLRELTQLARKSPGKIAYGSGGVGSANHLAAEFLQLIAKV
                     RFTHVPYKSAGLGLVGAMSGDVDMVIIVVSSGAAYVKDGRMRGLAVLDTKRSVSMPDV
                     PTSGEAGMPELQAVNWYALLAPAGTPRAIIERLHAESVKVMSPPDIQARLRAMGGEPE
                     ARSPDQTAAFLRAEYARWGKVIRAAGIKAE"
     gene            complement(1535..2236)
                     /locus_tag="AC158Q_07055"
     CDS             complement(1535..2236)
                     /locus_tag="AC158Q_07055"
                     /inference="COORDINATES: protein motif:HMM:NF012804.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR3339571.1"
                     /translation="MSAAGMTPDAMLTASALKAWRFLYRRGYIEGFGHLSVRLGADRF
                     LLTRHSLGLKADADDFLLVDMEGKKLSGRGELPGEYPIHLEILRSRPDVGSVIHYHGM
                     YSTAFTTSEQTLKPIHLMGTLFHDGIPVFPDPRLVSNRSRGEALAKALGPHRAVLLRA
                     HGAAVTGAGVEEAVGGAFLFEENARRACISAGLGKPVWMDDATAADAGAELLKNRGPF
                     RRVWAMVEAEDEEQG"
     gene            complement(2233..>3750)
                     /locus_tag="AC158Q_07060"
     CDS             complement(2233..>3750)
                     /locus_tag="AC158Q_07060"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HIR3339572.1"
                     /translation="IGPYMKDKLWVPPPDLEEKPNPYMNWETVNPLWWCPRGYALLRV
                     DTRGAGKSPGKSDPSSYQEALDCYDCIEWVAKLPWCNGNVGTCGISYHAAFQWRVASL
                     QPPSLKCILPWEGRADQYRDQAYHGGIFALGFIARWANNNTALHLLGKPRSYNPDSFD
                     NNLLWNYMRNDLDSEYWRLNSARWDRITVPVFSAGNWGGSSMHLRGNTEGYLSATASK
                     FRKLRIHTGTHYHPFHSEEARVDQLRWFDQWLKGIDTGILDEPPVKLEIRTGGSQKPY
                     PFRFEDDWPIPRTQWTKYHLRIDRAGTEDPQAVEGELTKTAPQKSATVSYQQSAPSRP
                     GKMPRGVSFETLPMAEDLEITGPIVLNIWVSSTGEDTDLFATLRNIDPGGKDVCEVGQ
                     MHEPMPCVTKGWLRASHRKLDPAKSLPYRPYHAHDERLWLKPGEAVECQVEIWPTSMV
                     FRKGHKLRLDVSPADGVGTQHFTHFHADYNQGAETTIHSGGNRASYLLLPVIPAK"
BASE COUNT          574 a         1265 c         1243 g          668 t
ORIGIN      
        1 gaggacaagg atcagcagga aaggaaatga gggccctgtc gctgccggtc atggtgcttg
       61 ccggctgcgc gaccctggcc gacgtcgata aagcccgcga ctcgtggcac ggcgcgcgct
      121 tcgacgaagt cgtgtcgcgt tggggccctc ccaaccgcag cgcgacgctc gccgacggcc
      181 gcgaaatctc gacctgggtg gccgagggcg tgtccggggg gctccccgtg gcaatcggcg
      241 tgttcggcgg ctccggagga ggcggcatcg gcgccagcat cgggctgccc ggcggcggcg
      301 agctgcagcg ctgcgagcgc acgctgactt tcaggaacga tcgcgtggtc gagcagacct
      361 ggctgggtca cccggccctg tgcagctcat tcaaaaaaga gtgatgcagc aggctcaagt
      421 ttcaagtttc aagttttttg gatttggacg ccgtgccttg taacttgtac cttgtacctt
      481 gtaccttgtt ccttgtacct ggcttattcg gccttgattc ccgccgcccg gatgactttc
      541 ccccagcgcg cgtactcggc gcgcaggaac gccgcggtct gatcgggcga gcgcgcctcc
      601 ggctcgccgc ccatcgcgcg caggcgcgcc tggatatccg gcggcgacat cactttcacc
      661 gactcggcat gcagccgctc gatgatcgcg cgcggagtgc cggcgggagc gagcagcgcg
      721 taccagttca ccgcctgcag ctccggcatc ccggcctcgc ccgacgtggg cacgtccggc
      781 atcgagaccg agcgcttcgt atccagcacc gccaggccgc gcatgcgccc gtccttcacg
      841 tacgccgcgc cgctcgagac cacgatgatg accatgtcga cgtccccgct catcgcgccg
      901 accagcccga ggcccgcgct cttgtacggc acgtgcgtga acctgacctt ggcgatcagc
      961 tgcaggaatt cggccgcgag atggttggcc gagccgacgc cgccggagcc gtaggcgatc
     1021 ttgccgggag acttgcgcgc gagctgcgtc aactcgcgca ggctcttcgc cggcaccgag
     1081 ggatggagaa acagcccgtt gggtatggtg gagagcagca cgatcggcgc gaagtcgcgc
     1141 atcggatcga agccgacccg cggctggatg aacgggttcg tcagcagcgg caccgcgccc
     1201 atcagcagcg tgtagccgtc gggcgcggag cgggcggcgg cctcgaagcc gatgctgccc
     1261 gcggcgccca gccgcgggtc gaccagcacc tgctgcccca gcgcgggcgt caactgctgc
     1321 gccaccatgc gcgccatcag ctccgagccg ccggagaacc cggtgatgat cctcaccggc
     1381 ttcacgggat actgttgcgc gctcgcgaca cctgccgcga gcacgcaacc aaggatgagg
     1441 gatgagggat gagggatgaa attaaaccag ccaaaccctt ttgcctcttg ctttgtgcct
     1501 cttgatttat tcctcatccc tccgccctcc tccctcatcc ctgttcttcg tcctctgcct
     1561 cgaccatcgc ccacacgcgg cggaacggcc cgcggttctt caggagctcg gcaccggcat
     1621 cggcggcggt cgcatcgtcc atccacacgg gcttgccgag gccggcgctg atacaggcac
     1681 ggcgcgcgtt ctcctcgaac aggaacgcgc cgcccaccgc ctcctccacc ccggcgccgg
     1741 tgaccgccgc gccatgggca cgcaggagca ccgcgcggtg cggacccagc gccttcgcca
     1801 gcgcctcgcc gcggctgcga ttgctcacca gccgcgggtc gggaaacacc ggaatgccgt
     1861 cgtggaacag cgtgcccatg agatggatcg gcttcagcgt ctgctcgctg gtggtgaatg
     1921 ccgtcgaata catgccgtga taatgaatca cgctgccgac gtcggggcgc gaccgcagga
     1981 tttccaggtg tatcgggtat tcgcccggca gttcgccgcg gccggagagc ttcttgccct
     2041 ccatgtccac gagcaggaaa tcgtccgcgt ccgccttgag tcccagcgaa tggcgggtga
     2101 gcagaaaccg gtccgcaccg aggcgcacgc tcagatggcc gaagccctcg atgtatcccc
     2161 gccggtagag aaagcgccag gccttgagcg cgctggccgt cagcatcgcg tcgggcgtca
     2221 tcccggccgc gctcatttcg cgggaatcac cggcagcagc aggtacgacg ccctgttgcc
     2281 gccggaatgg atcgtggtct cggcgccctg gttgtaatcg gcgtgaaagt gagtgaagtg
     2341 ctgggtgccg acgccgtcgg cgggcgagac gtcgaggcgc agcttgtggc ccttccggaa
     2401 caccatgctg gtcggccaga tctcgacctg gcattccacc gcttcgccgg gcttgagcca
     2461 gagccgctcg tcgtgggcgt gatacggccg gtacggcagg gacttcgccg ggtcgagctt
     2521 gcggtgcgaa gcgcgcagcc agcccttggt cacgcagggc atcggctcgt gcatctggcc
     2581 cacctcgcac acgtccttgc cgccgggatc gatattgcgc agcgtcgcga agagatccgt
     2641 gtcctctccc gtgctcgaca cccagatatt gagcacgatc ggccccgtga tttccaggtc
     2701 ctcggccatc ggcagcgtct cgaaggacac gccgcgcggc atcttgccgg ggcgggacgg
     2761 tgcgctctgc tggtagctca ccgtggcgga tttctgcggc gccgttttcg tcagctcgcc
     2821 ctccaccgcc tgcgggtctt ccgtgccggc gcggtcgatc ctcagatggt atttcgtcca
     2881 ttgcgtgcgc gggatcggcc agtcgtcctc gaagcggaat gggtacggct tctggcttcc
     2941 cccggtgcgg atctcgagct tgaccggcgg ctcgtcgagg atgccggtgt cgatcccttt
     3001 cagccactgg tcgaaccagc gcagctggtc gacgcgtgcc tcctccgaat ggaacggatg
     3061 gtaatgcgtt ccggtgtgga tgcgcagctt cctgaacttc gacgccgtgg cgctcaggta
     3121 gccctcggta ttgccgcgca ggtgcatgga ggaacccccc cagttgccgg cgctgaacac
     3181 cggcacggtg atccggtccc agcgcgcgct gttcaggcgc cagtactcgg aatcgaggtc
     3241 gttcctcatg tagttccaca gcaggttgtt gtcgaacgag tccgggttgt agctgcgcgg
     3301 cttgccgagc aggtgcaggg ccgtgttgtt gttggcccag cgcgcgatga agcccagcgc
     3361 gaagatgccg ccgtgatagg cctggtcgcg gtactggtcg gcgcgcccct cccagggcag
     3421 gatgcatttc agcgacggcg gctgcaggct cgcgacccgc cactggaagg ccgcgtggta
     3481 cgagatgccg caggttccga cgttgccgtt gcaccacggg agtttcgcga cccattcgat
     3541 gcagtcgtag cagtcgagcg cttcctggta cgagctcggg tccgattttc cgggcgactt
     3601 gccggcgccg cgcgtatcca cgcgcagcag cgcgtagccg cgcgggcacc accagagcgg
     3661 gttcaccgtt tcccagttca tgtagggatt gggcttctcc tcgaggtcgg gcggcggcac
     3721 ccacagcttg tccttcatgt acggcccgat
//
LOCUS       DBNGSG010000272         6100 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4672273, whole genome shotgun sequence.
ACCESSION   DBNGSG010000272 DBNGSG010000000
VERSION     DBNGSG010000272.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6100)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6100)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6100
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4672273"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1147)
                     /gene="bioF"
                     /locus_tag="AC158Q_07065"
     CDS             complement(<1..1147)
                     /gene="bioF"
                     /locus_tag="AC158Q_07065"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HIR3339573.1"
                     /translation="MKREERAFAPFSALTTELAELDCRGLKRSRRVLETPQGGRVRVD
                     GREYTAFCSNDYLGLAAHPELIEAAREGAARFGVGAGASHLILGHTMAHHELEQRLAV
                     FAGLPRALLFSTGYLANLAAVTALVGREDAVFADRLNHASLNDAALLARAQFKRYAHA
                     DLDSLERQLAASRARRKLVVTDAVFSMDGDIAPVPGLVALCERHDAWLLLDDAHGFGV
                     LGSEGRGTLTHFGVNSPRVIYMATLGKAAGVSGAFVAGETEVVEMLIQRARAYIYTTA
                     SPPMLAVTLMKSLELIRDGESRRDHLRRLIALLRQQLKPARWRLMPSQSAIQPLVIGP
                     NGEAVAVSETLAHGGLLIPAIRPPTVPQGTARLRISLSAAHSIEDIER"
     gene            complement(1144..2151)
                     /gene="bioB"
                     /locus_tag="AC158Q_07070"
     CDS             complement(1144..2151)
                     /gene="bioB"
                     /locus_tag="AC158Q_07070"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HIR3339574.1"
                     /translation="MNTPTFNESRAIAADNTPATPRWSARQVEELFDSPFNDLIYRAH
                     GVHRSHFDPNAVQLSTLLSIKTGGCPEDCGYCPQAARYHTGVANEELMTVEQVTAAAR
                     LAKASGATRFCMGAAWRGPRQRELERVIEMVKAVRAEGLESCATLGLLREGQAEQLRA
                     AGLDYYNHNIDTAPEFYGEIITTRTQDDRLETLDRVRHAGLHVCCGGIVGMGESRRTR
                     AALIAQLAGMEPYPESVPINNLVQVPGTPLAGTEPLDPLEFVRTIACARITMPKAMVR
                     LSAGRQAMPEAIQALCFFAGANSIFYGEKLLTTGNPDVEQDRALFARLGLRPMEARSE
                     V"
     gene            2223..2930
                     /locus_tag="AC158Q_07075"
     CDS             2223..2930
                     /locus_tag="AC158Q_07075"
                     /inference="COORDINATES: protein motif:HMM:NF039185.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
                     /protein_id="HIR3339575.1"
                     /translation="MSNFVPGILNNCLAFARSLLPQSCQLCGADSGSAALCPPCLEDL
                     PWLARARCAVCALPLTSGRVCGACLERPPRFNRVEAVFAYRFPADTLIHAYKYGRRLA
                     LARALGGLLARQVARDVDAIVPMPLARGRLAERGFNQALEIARVAAARTGVALLPHAC
                     RKVVETPAQATLPWEERARNVRRAFVCDSELEGKRVAVVDDVLTTGATLNELARVLRK
                     AGAVEVRGWVVARTLPR"
     gene            complement(3009..4166)
                     /locus_tag="AC158Q_07080"
     CDS             complement(3009..4166)
                     /locus_tag="AC158Q_07080"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3339576.1"
                     /translation="MSVINQLLIDLDRRRASGAERSNLPNHVRALPQAERPTPWGWIA
                     AGGAGVAVVLAMAWALISGFDWTQQRAAAAPPVGTEIVIEKVVAASAGVTADARPGDD
                     SEGVRKEALASRLSFDLSSPPASAAREGADTPPPLSTARVIGQAGSDASASSEPSPVP
                     AGRPAAARTPAVASARVAAGAPSAKAEIQKEVRQPSSRDLAENEYRKATALLHQGRLA
                     ESEEGFRATLNLLPGHLGARQALVGLLLDARKYGDAERVLQEGLALAPAQTGFAMTLA
                     RLQVDRGETAQGIATLRKGLEHAQGGADYLAFLAALLQRQGRHEEAIEQFQAALRTRP
                     AAGVWWLGLGISLQSVNRTADAQEAYRRARASNNLSPELAAFAEQRLRQLQ"
     gene            complement(4163..5023)
                     /locus_tag="AC158Q_07085"
     CDS             complement(4163..5023)
                     /locus_tag="AC158Q_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExeA family protein"
                     /protein_id="HIR3339577.1"
                     /translation="MPFGITPDTSFAYACNTHQEALNTLLVAVKNGEGFIKITGEVGT
                     GKTLLCRRFLAMLDQKHVSAYIPNPYLEPRTLLFALAEELGVALPRGTDQHQLMKEIT
                     RALLNFARQGKTVVLCLDEAQAMPVETMEALRLLTNLETEKRKLIQVVLFGQPELDRK
                     LEHASIRQLRQRITFQYRLMALGRDETDQYVAHRLRVAGYRGNRLFARQATRLAYRYS
                     GGVPRLINIMAHKAMLLAYGEGVQQVQPSHLRKAAADTPVPRQRRPWRWLGFAMLLLS
                     VGGLGWAVLS"
     gene            complement(5072..>6100)
                     /gene="mshL"
                     /locus_tag="AC158Q_07090"
     CDS             complement(5072..>6100)
                     /gene="mshL"
                     /locus_tag="AC158Q_07090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus (MSHA type) biogenesis protein MshL"
                     /protein_id="HIR3339578.1"
                     /translation="SGVIVVRALPAEMRSVESFLKAMQLIVERQVVLEAKIISVSLGD
                     SFGAGINWAAFRDGSTRAAGGMIRPGTTIGNTGTLSTPTARSPDGSLAADSAFTATLG
                     SVASLAAGVGAPGSIFGLALQTSNFAALLTFLETQGTVNVLSSPRVATINNQKAVLKV
                     GTDDFFVTNVSTTSTTSGTSTTISPTITVQPFFSGIALDVTPQIDDNNNVILHIHPQV
                     SEVVERRKVVDLGTLGTFTLPLASSSINETDTIVRVQDGNIVAIGGLMRQESTNDTSQ
                     VPGLGDQPGFGALFRQRGRTNIKSELVILLKPTIVHSDREWQQDLTDTRDRIRALEPP
                     QPAQPTER"
BASE COUNT          948 a         2051 c         2087 g         1014 t
ORIGIN      
        1 ggcgctcgat gtcttcaatc gaatgagccg ccgacagcga gatgcgcagg cgtgcagtgc
       61 cctgcggcac ggtgggcggc cggattgccg gtatcagcaa tccgccgtgc gccagcgttt
      121 cggaaacggc gacggcctcg ccgttgggtc cgatcacgag cggctgtatc gccgattgcg
      181 acggcatcag ccgccagcgc gccggtttca gttgttgccg cagcagcgcg atcaggcgcc
      241 gcaggtgatc ccgacgcgat tccccgtcgc gtatgagctc taagcttttc atgagcgtca
      301 ccgccagcat cggcggggat gcagtcgtat agatataggc ccgggcgcgc tgtatgagca
      361 tctccaccac ttccgtctcc cccgcgacaa aagcgccgga gacgcccgcg gccttgccca
      421 gcgtcgccat gtaaatcacg cgcggggaat tcacgccgaa gtgcgtgagc gtgccccgcc
      481 cctcgctccc cagcaccccg aagccatgag cgtcatcaag cagcagccag gcgtcgtggc
      541 gctcgcacag ggcaaccagg cccggcaccg gcgcgatgtc cccgtccatg ctgaacaccg
      601 catcggtgac gaccagcttg cggcgcgccc tggatgcggc cagctgccgt tcgagcgaat
      661 cgaggtcggc gtgcgcatag cgcttgaact gcgcgcgcgc gagcagcgcc gcgtcgttga
      721 gcgatgcgtg gttcaggcgg tcggcgaaga ccgcgtcttc gcgtcccacc agcgccgtca
      781 ccgcggcgag gttggccaga tagccggtcg agaaaagcaa cgcgcgcggc agtcccgcga
      841 ataccgccag ccgctgctcc agctcgtgat gcgccatggt atgcccgagg atcaggtggg
      901 acgcaccggc gccgacgcca aaacgcgccg ccccctcgcg cgcagcctcg atcaattcgg
      961 gatgggcggc aaggccgaga taatcgttgc tgcagaacgc ggtgtactcg cgcccatcga
     1021 ccctgacacg gccaccctgc ggcgtctcta gcacccggcg cgagcgcttg aggccgcggc
     1081 aatcgagctc ggcaagttct gtagtgagcg cggaaaaggg cgcgaacgcc cgctcctcac
     1141 gcttcacacc tcactcctcg cctccatggg gcgcaggccg agcctggcga acagcgcgcg
     1201 atcctgctcg acgtcggggt tgccggtggt gagcagcttc tcgccataga agatcgagtt
     1261 ggcgcccgcg aaaaagcaca gcgcctggat cgcctccggc atcgcctgcc ggccggcgga
     1321 gagccgcacc atggccttcg gcatggtgat gcgcgcgcag gcgatggtgc ggacgaactc
     1381 gagcgggtcg agtggctccg tgccggcgag cggggtaccc ggcacctgca ccaggttgtt
     1441 gatcggcacc gattcggggt acggctccat gccggccagc tgcgcgatca acgccgcgcg
     1501 cgtgcggcgc gactcgccca tgccgacgat cccgccgcaa cagacgtgca ggccggcgtg
     1561 gcgcacgcga tcgagcgtct ccaggcgatc gtcttgcgtg cgcgtggtga tgatctcgcc
     1621 gtagaactcg ggcgcggtgt cgatgttgtg gttgtagtag tcgaggccgg cggccctgag
     1681 ctgctcggcc tggccttccc tcagcaatcc gagcgtggcg cagctttcaa gcccttccgc
     1741 gcgcaccgct ttcaccatct cgatcacgcg ctcgagctcg cgctgcctgg ggccgcgcca
     1801 ggcggcaccc atgcagaagc gcgtggcgcc gctggctttc gcgagccgcg ccgccgccgt
     1861 cacctgctcg accgtcatca gttcctcgtt ggcgacgccg gtgtgatagc gcgcggcctg
     1921 cggacaatag ccgcagtcct cgggacagcc accggtcttg atggagagca gcgtcgacag
     1981 ctgcaccgca ttgggatcga aatggctgcg gtgcacgccg tgcgcgcgat aaatgaggtc
     2041 attgaacgga gaatcgaaca attcctcgac ttgccgcgcg ctccagcgcg gcgtggccgg
     2101 tgtgttgtca gcggcgatgg cacgggactc gttaaaggta ggtgtgttca ttcggttaac
     2161 cgatgagggc aaggaaaatc atgcgttttg aatcacttgc atcttcgggc cgggcgcgcc
     2221 gcttgtcaaa tttcgtccct ggaatattga acaactgcct cgcttttgca cgctccctgc
     2281 tgccgcaatc gtgtcagttg tgcggcgctg acagcgggag cgcggccctg tgcccgccgt
     2341 gcctggagga cctgccgtgg ctggcgcgcg cccgctgcgc ggtgtgcgcc ctgcccctga
     2401 ccagcggccg ggtatgcggc gcctgcctcg agcgcccgcc gcgcttcaac cgggtggagg
     2461 cggttttcgc ctaccgcttc ccggcggaca cattgatcca cgcctacaag tacggcaggc
     2521 ggctggcgct ggcccgcgcg ctgggcgggt tgctcgcgcg gcaggtcgcg cgcgatgtgg
     2581 atgcgatcgt gccgatgccg ctcgcgcgcg ggcggctcgc cgagcgcggg ttcaaccagg
     2641 cgctggaaat cgcgcgggtg gcagcggcgc gcaccggcgt cgccctgctg ccgcacgcct
     2701 gccgcaaggt cgtcgagacg cccgcgcaag ccacgctgcc gtgggaagag cgggcgagaa
     2761 acgtgcgccg ggcattcgtg tgcgattccg aactcgaagg caagcgcgtc gccgtggtgg
     2821 atgatgtgct cacgaccggg gcgacgctga acgagctggc gcgcgtattg cgcaaggcag
     2881 gcgccgtgga agtgcgcggg tgggtggtgg cgcgaaccct gccacgataa cttcaggatc
     2941 caggaatccg gagtcaggat tcagaaaccg gcttaccctg aatccgggat cctgaatccc
     3001 gaatcctctt attgaagttg cctgaggcgt tgctcggcga aggctgccag ttcgggactg
     3061 aggttgttgc tcgcgcgcgc gcggcggtag gcttcttgcg catcggcggt gcgattgacc
     3121 gactgcagcg aaatgccgag cccgagccac cacacgcccg cggcgggccg cgtccgcagg
     3181 gcagcctgaa actgctcgat cgcctcctcg tgccggccct ggcgctgcag cagcgcggcg
     3241 aggaaggcga ggtaatcggc gccgccttgc gcgtgctcca gcccttttct gagagtggcg
     3301 atcccctggg cggtttcccc gcggtccacc tgcagccgcg cgagcgtcat ggcgaacccg
     3361 gtctgcgccg gagcgagcgc caacccttcc tgcagcacgc gttcggcgtc gccgtacttt
     3421 ctggcgtcca gcagcaatcc caccagcgcc tggcgcgcgc cgaggtgccc cggcaggaga
     3481 ttcagggtcg cccggaagcc ctcctcggat tcggcgaggc gcccctggtg cagcagagca
     3541 gttgccttgc ggtactcgtt ctctgccaga tcgcgcgacg aaggttgccg cacctccttc
     3601 tggatctccg ccttggcgga aggcgcgccc gccgccactc tggcagacgc aacggcgggc
     3661 gtcctggcgg ccgcgggccg cccggcagga acgggggacg gctcgctgct cgccgaggca
     3721 tccgaacccg cttgtccgat cactcgcgcc gtcgacaacg gagggggcgt atccgcaccc
     3781 tcgcgcgccg cggaagcggg agggctggac agatcgaaac tcaagcgcga agcgagcgcc
     3841 tccttgcgca cgccctcgct gtcatcgccc ggtcttgcat ccgcggtcac gcccgcggag
     3901 gcggccacca ctttttcgat gacgatctcg gtgccaaccg ggggggcggc cgccgcgcgc
     3961 tgttgcgtcc agtcgaatcc ggaaatgagc gcccacgcca tggcaagcac caccgcaacg
     4021 cccgcgccac cggccgcgat ccagccccag ggggtcgggc gctcggcctg cggcagcgcc
     4081 cgcacgtgat tgggcaggtt gctgcgctcg gccccggacg cccggcgcct gtccagatcg
     4141 atcaggagct ggttgattac gctcatgaca gcaccgccca gccgagaccg ccaaccgaca
     4201 gcagcaacat ggcaaatcca agccaccgcc acggccgccg ctggcgcggc accggcgtat
     4261 ccgccgcggc cttgcgcagg tgggacggct gcacctgctg cacgccctct ccgtaagcca
     4321 gcagcatggc cttgtgcgcc atgatgttga tgagccgcgg caccccgccg ctgtagcggt
     4381 atgcgagtcg cgtcgcctgc cgcgcgaaga ggcggttgcc acggtagccc gccacgcgca
     4441 gccggtgcgc gacgtactgg tccgtttcat cccgaccgag cgccatcagc cggtactgga
     4501 aggtaatgcg ctgcctcaac tgacggatcg aagcgtgctc gagcttgcgg tcgagctcgg
     4561 gctggccgaa gaggaccacc tggatcagct tgcgcttctc ggtctcgagg ttcgtcagca
     4621 ggcgcagcgc ttccatcgtc tcgaccggca tcgcctgcgc ctcgtccagg cacagcacca
     4681 ccgtcttgcc ctgccgcgcg aagttgagca gcgcgcgcgt gatctccttc atcagctgat
     4741 gctggtcggt ccccctgggc agcgcgaccc cgagctcctc ggccagcgcg aacaggagcg
     4801 tgcgcggctc gaggtaggga ttgggaatgt aggccgagac gtgcttctgg tcgagcatgg
     4861 cgaggaagcg gcggcagagc agagtcttgc cggtgcccac ctcgccggtg atcttgatga
     4921 agccttcccc gttcttgacc gccaccagca gcgtgttgag cgcctcctgg tgggtattgc
     4981 aggcataagc gaagctcgta tcgggcgtga tcccgaatgg catttcgcga aggccgaagt
     5041 ggctgcggta catggcgtca atccgactgt atcaccgctc ggtcggttgt gccggctgcg
     5101 gcggctccag cgcgcggatg cggtcccgcg tatcggtcag gtcctgctgc cattcgcggt
     5161 cgctgtgaac gatggtgggt ttcagcagga tcacgagctc gctcttgata ttggtcctgc
     5221 cccgctgccg gaaaagcgcg ccaaagccgg gctggtcgcc gaggccgggc acttgcgagg
     5281 tgtcattcgt cgactcctgg cgcatgaggc cgccgatcgc cacgatgttg ccgtcctgca
     5341 cgcgcacgat ggtgtcggtc tcgttgatgc tgctggaagc gagcggcagc gtgaaggtgc
     5401 cgagcgtgcc caggtccacc accttgcgcc gctccaccac ttcgcttacc tgcggatgga
     5461 tgtgcaggat gacgttgttg ttgtcgtcga tctgcggcgt cacgtcgagc gcgatgccgg
     5521 agaagaacgg ctgcacggtg atggtcggcg agatcgtcgt gctggtgccg ctggtggtcg
     5581 aggtggtgga gacgttggtg acgaagaagt catcggtgcc gaccttgagc accgccttct
     5641 ggttgttgat ggtcgccacg cgcgggctgg acagcacgtt gaccgtgccc tgggtctcga
     5701 ggaacgtcag caatgcggcg aaattgctgg tctgcaacgc gaggccgaat atgctgccgg
     5761 gcgcccccac ccccgcggcg agcgaggcca cgctgccgag cgtggcagtg aaagcggagt
     5821 cagcggccag cgatccgtcc ggcgagcgcg ctgtcggcgt ggacagcgta ccggtgttgc
     5881 cgatggtcgt tcccgggcga atcatgccgc cggcggcgcg ggtgctgccg tcccggaacg
     5941 ccgcccagtt gatgccggcc ccgaagctgt cgccgagact gaccgagatg attttcgctt
     6001 cgaggaccac ctgccgctcg acgatcagct gcattgcctt gagaaaagac tcgaccgacc
     6061 gcatctccgc ggggagcgcg cgcactacga tcaccccgga
//
LOCUS       DBNGSG010000273         6979 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2633887, whole genome shotgun sequence.
ACCESSION   DBNGSG010000273 DBNGSG010000000
VERSION     DBNGSG010000273.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6979)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6979)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6979
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2633887"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="AC158Q_07095"
     CDS             complement(<1..1115)
                     /locus_tag="AC158Q_07095"
                     /inference="COORDINATES: protein motif:HMM:NF006514.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR3339579.1"
                     /translation="MARPGPGARAIRLARRMAEIEPFHVMVLLGRARELEAQGRSIIH
                     MEIGEPDFPTPEPIVRAGIRALEKGELYYTPTPGLPPLREAIAGYYRSRYGVEVSPSR
                     ILVTTGSSAALMLTCAALVDPGDEILLADPGYPANRHFVRVVEGVPRAVPVDAASGYQ
                     LAPEHLERYWSPRTVAALVATPSNPTGTLISTERLAAMAEIARGKGGTLIVDEIYHGL
                     VYDDDTTTALALADDVFVINSFSKYFNMTGWRLGWMVAPEPYVRELDKLAQNLYLAAP
                     TPAQYAALAAFEPETLAILAGRAQEFKARRDYLVPALKRLGFDIPVTPQGAFYVYAGC
                     GKLTQDSFGFARDLLERAGVAITPGIDFGSNAPERHVR"
     gene            complement(1121..2077)
                     /locus_tag="AC158Q_07100"
     CDS             complement(1121..2077)
                     /locus_tag="AC158Q_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012358222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HIR3339580.1"
                     /translation="MKTLLVAMVAAVGLATAAHGQEVKLSISTGGTGGVWYPMGGAMA
                     NVLTKHMPGGAAVTAEVTGGSVANINLLASRKSEIAFSMVDAAWDGLHGTGKFKAKVP
                     MRTIAVVHPLVMHVVTAEGRGIETMADLKGKRISTGSPGSGTEVMALRILEASGINPD
                     KDVVRERLGVSEAANALKDRKIDAFMHAAGVPLPAVTDVAASPGIRIKLVDHAGALKA
                     MTSKYGPIYAAGTIPARSYSGQEKDNQTVNVWGVLLVHESMDEKLVYDILKTLFDRHK
                     DIVIAHREAANMTLANQSIGATAVPWHPGAVKYFAERGVTAR"
     gene            complement(2231..3145)
                     /locus_tag="AC158Q_07105"
     CDS             complement(2231..3145)
                     /locus_tag="AC158Q_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR3339581.1"
                     /translation="MEHLMAMAVFARVVEAKSFSGAAQRLGISKSAVSKHVSGLERAL
                     KARLLNRTTRRLSLTEVGAQFYEHCTRMLAEAEAAELAVSRLHAAPRGVLKVTSPAAF
                     GHLMLAPAIPDLLARYPEMSVQIAMNDRPVDLAEEGFDVAIRLADRLPPNVVARKLMT
                     VRWVVCASPAYLARHGTPRTPQELQRHNCLFYSFLESAIEWRFRSARGETRVLVKGNF
                     TANNSEAIRETALKGLGVALVPGFSVDADLRDGRLKEVLGGYDVEGAVATDVYALYLP
                     TRYLSPKVRAFVDFFVERFGAGFASDKP"
     gene            3302..3481
                     /locus_tag="AC158Q_07110"
     CDS             3302..3481
                     /locus_tag="AC158Q_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339582.1"
                     /translation="MEATIGRLPAQRIEAAAADWLRATRRLLRALARGAGHVLVHYSR
                     AAILLRGGERPFRRL"
     gene            complement(3501..4187)
                     /locus_tag="AC158Q_07115"
     CDS             complement(3501..4187)
                     /locus_tag="AC158Q_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HIR3339583.1"
                     /translation="MVRRTKDEAQETRNRILDAAEHLFCERGVSRTSLEDIAQAAGVT
                     RGAIYWHFKDKSDLFAGMVNRVTLPMEAMVARASDESVEDPIASLKACAVNALKRTAT
                     DPQVQRVFDVVTHKCEYLGEMAGVNNRISAVQKGCVDRAEQTIRNAVRRGLLPAGVNP
                     RLAAVGLDAMLYGLISNWLANPGYLPLERHAEAMIDLYLDGLRNGGARRAHSPAKRRP
                     RGPARRSKAR"
     gene            4354..5631
                     /locus_tag="AC158Q_07120"
     CDS             4354..5631
                     /locus_tag="AC158Q_07120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3339584.1"
                     /translation="MNTLSSISRGGLRAALPAIFSMALALLAAGCQPAGGPPGGFPPP
                     LVTVSVVEPRDVPVTYEYVAQTAGYREIEVRARVTGILLKRNYKEGATVRQGESLFTI
                     DPAPFQVALAKAEADLGVAEARFAQAKRESGRLKPVIDARAASQKELDDAVSAEQVAD
                     AEVRSARARVAEAKLNLDYTRVESPISGITSRAVVSEGALVSGPNVLLTTVTQTDPMY
                     VIFGIPDREFLAIRRDVAAGRLKIPEGGRFKATIKLSDGSFYDKSGTLNFADVRVNGQ
                     TGTSEARAEVANSGNLLRAGEFARVVLEGAMRPGAMVIPQRAVLENPKGKYVFIVTAD
                     GKSEPRPVEVGDWTGDGWIIISGLNPGDRVVVDGVMKLSLMPPGSPVKIADDAAAPED
                     KKGPPEKKGPADKKGAEGSKAPAVKKAPPEKKV"
     gene            5643..>6979
                     /locus_tag="AC158Q_07125"
     CDS             5643..>6979
                     /locus_tag="AC158Q_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3339585.1"
                     /translation="MISRFFIDLPIFSAVLSIFLVIAGLASMRVLPIAQYPEIAPPVV
                     TVRAIYPGASAQVLEQTVAAPLENQITGVENMLYMSSTSTSSGVVEIQVTFEIGADVD
                     TAALNVNNRVKQVEPRLPLEVRRQGVTVEKGSSSFLQVIAFTSPDGSRDDLFISNYVT
                     LNVLDSLKRIPGTTNVQIFGAKDYAMRIWVRPDRLAQLKVSPSDLIRAMNEQNAQFAA
                     GKIGQSPTSGPQELVYTITTKGRLTEPAEFENIVIRANPDGSILRMKDVARVELGSRD
                     YEFMGRVNGKSATLVGIFLQPNANAIATGDRVKRAMGDLASRFPPGLEHHVVYDTTRF
                     VEVSIREVVITLAEAMALVFLVVFLFLQNWRATLIPFAAVPVSLIGTFAGLVMLGYSI
                     NTLTLFGMVLAIGIVVDDAIVVLENVERIMHEEHLQAREAAIKAMKEVTSPIIA"
BASE COUNT         1198 a         2332 c         2282 g         1167 t
ORIGIN      
        1 cgcacgtgcc gctcgggggc atttgaacca aaatcgattc ccggcgtgat cgcgacgccg
       61 gcccgctcca gcaggtcgcg cgcgaagccg aaactgtcct gcgtcagctt cccgcagccg
      121 gcgtagacgt agaacgcgcc ctgcggcgtc accggaatgt cgaatcccag ccgcttcagc
      181 gccggcacca ggtaatcgcg ccgcgccttg aattcctgcg cgcgcccggc cagtatggcc
      241 agcgtttccg gctcgaacgc ggcgagcgcg gcgtactgcg ccggcgtcgg cgcggcgaga
      301 tagaggttct gggcgagctt gtcgagttca cgcacgtaag gctccggcgc taccatccag
      361 cccaggcgcc agccggtcat gttgaagtac ttggagaaac tgttgatgac gaagacatcg
      421 tcggcaagcg ccagcgccgt cgtggtgtcg tcatcataaa caagcccgtg atagatctcg
      481 tccacgatca gggtgccgcc cttcccgcgc gcgatctccg ccatcgccgc gagacgctcg
      541 gtcgagatca gcgtgccggt gggattcgaa ggagtggcga cgagcgcggc gacggtgcgc
      601 gggctccaat agcgctcgag atgttccggg gcgagctggt acccgctcgc ggcatccacc
      661 ggcaccgcgc gcggcacgcc ctccaccacc cgcacgaaat ggcggttggc gggatagccg
      721 ggatcggcga gcaatatttc gtctcccgga tcaacgagcg ccgcgcaggt cagcatcagc
      781 gcggcggatg agccggtcgt gacgaggatg cgcgaggggg aaacctccac gccgtagcgc
      841 gagcggtagt agccggcgat cgcctcccgc aatggcggca gcccgggcgt gggcgtgtag
      901 taaagttcgc ccttctccag cgcccggatc ccggcgcgca cgatcggttc cggcgtcggg
      961 aaatccggct cgccgatttc catgtggatg atggagcggc cctgcgcctc cagctcgcgc
     1021 gcgcggccaa gcagcaccat cacgtggaac ggctcgatct ccgccatccg ccgcgcgagg
     1081 cggatggcgc gcgcccccgg tcctgggcgc gccatcggcg ctacctggcg gtcactccgc
     1141 gctcggcgaa gtacttcacc gctcccggat gccagggcac cgccgtcgcg ccgatcgact
     1201 ggttcgccag cgtcatattg gcggcttcgc ggtgcgcgat gacgatatcc ttgtgccggt
     1261 cgaacagcgt cttcaggatg tcgtagacca gcttttcatc catgctctcg tgaaccagca
     1321 gcacgcccca gacgttgacc gtctggttgt ccttctcctg cccgctgtag gagcgcgcgg
     1381 gaatggtacc cgccgcgtag atcgggccgt atttcgaggt catcgccttg agggcgccgg
     1441 catggtccac gagctttatc cggatccccg ggctggcggc aacgtcggtc accgccggca
     1501 gcgggacccc ggccgcgtgc atgaaggcgt cgatcttgcg gtccttcagc gcgttggccg
     1561 cttcggaaac acccagccgc tcgcgcacga cgtccttgtc ggggttgatg ccggacgctt
     1621 cgagtatgcg cagcgccatc acttcagtcc cgctcccggg cgagccggtg gaaatgcgct
     1681 tgcccttgag atcggccatc gtctcgatgc ccctgccctc ggcagtgacg acatgcatga
     1741 cgaggggatg gaccaccgcg atcgtgcgca tcggcacctt cgccttgaac ttgccggtgc
     1801 cgtggaggcc gtcccacgcc gcgtcgacca tcgagaaggc aatctcggac tttctcgaag
     1861 cgagcaggtt gatgttggcc acgctgccgc cggtgacttc ggcggtcacc gcggcgccgc
     1921 cgggcatgtg cttggtgagc acgttcgcca tcgcgccgcc catggggtac cagacgccgc
     1981 cggtgccccc ggtcgagatg gaaagcttga cttcctggcc gtgagctgcg gttgccaatc
     2041 cgaccgcggc caccattgca acgagcaacg tcttcatgtt ctctcctccg ggaaagcgag
     2101 tcgtttctgg aatgatggat tgaacggcaa tggctatgct acgccaaaaa gcgatcaggg
     2161 ggacccgccg agggatgagg gatgaatccg gcccccgccg cattgcgcgc cggggcctgc
     2221 tgctcgcgaa tcaaggttta tccgatgcaa aaccggcgcc gaatcgctcg acaaaaaagt
     2281 ccacgaacgc ccgcaccttg ggcgagagat agcgcgtggg cagatacagg gcgtaaacat
     2341 cggtggcgac ggcgccttcg acgtcatagc cgcccagcac ctccttcagc cgcccatcgc
     2401 gcaggtcggc gtccacggaa aaaccgggca ccagcgcgac accgaggcct ttcagagccg
     2461 tctcccggat tgcctccgag ttgttcgccg tgaagttacc tttcaccagg acccgggttt
     2521 ccccgcgggc gctccggaaa cgccattcga tcgccgattc gagaaacgag tagaacaggc
     2581 agttgtggcg ctgcagttcc tgcggcgtgc gcggcgtccc gtgccgcgcg aggtaagcgg
     2641 gggacgcgca gacaacccag cgcacggtca tcagcttgcg cgcaacgacg ttgggcggca
     2701 gccggtccgc gagccggatc gcgacgtcga atccctcctc ggccaggtcc accggccggt
     2761 cgttcatcgc gatctgcacg ctcatctcgg gataccgcgc gagcaggtcc gggatggcag
     2821 gcgccagcat caggtggccg aacgcagcgg gcgaggtcac tttcagcact ccgcgcggcg
     2881 cggcgtgcag gcgcgagacc gcgagttccg ccgcttcggc ctccgccagc atgcgcgtgc
     2941 agtgctcata gaactgcgcg cccacttcgg taaggctcag gcgccgcgtg gtgcggttga
     3001 gcagccgcgc cttgagcgcg cgttcgaggc ccgagacgtg cttgctcacc gccgacttgg
     3061 atatccccag ccgttgcgcc gcgcctgaaa agctctttgc ctccaccacc cgggcgaata
     3121 ccgccatggc catcaggtgc tccatcgtca atcctattgt cttttaatag aaacaataat
     3181 attccatttt ggatgattgt aaatacccgg aaacggtgca aactctgttc aacacagcac
     3241 cggaggtggg gcggaacgcc ccgccgttca ccggatagct gggccggaaa ggagcacgat
     3301 catggaagca actattggaa gactgccagc acaacgaatc gaagccgcgg ccgcagactg
     3361 gctgcgcgcg acgcggcggc tgttgcgcgc gctggcgcgc ggagcaggac acgtgctggt
     3421 tcactacagc cgcgccgcga ttctgcttcg cggcggcgag cgcccgttcc ggcgcctttg
     3481 aacccgccgg cctggcgagg ctagcgcgcc ttcgaacggc gcgctggtcc gcgcggacgg
     3541 cgtttcgcgg gcgaatgcgc gcgtcgcgcg cccccgttcc gcagcccgtc cagatagagg
     3601 tcgatcatgg cctcggcgtg gcgctcgagc ggcagatagc cgggattggc aagccagttg
     3661 gaaatcagcc cgtagagcat ggcatccagg ccgacggccg ctagccgcgg gttcacgcct
     3721 gccggcagca ggccgcgccg gaccgcgttg cgtatggtct gctcggcgcg gtccacgcac
     3781 cctttctgga cggcgctgat ccggttattg accccggcca tctcgcccag gtactcgcac
     3841 ttgtgcgtga ccacgtcgaa cacgcgctgg acctgagggt cggtcgccgt gcgcttcagc
     3901 gcgttcacgg cgcaggcctt caaagaggcg atcgggtcct cgacggattc gtcgctcgcg
     3961 cgcgccacca tcgcctccat cggcagggtc acgcggttca ccatgccggc gaagagatcg
     4021 ctcttgtcct tgaagtgcca gtagatcgcg ccgcgcgtca cgcccgcggc ctgggcgatg
     4081 tcctcgagcg acgtgcgcga aaccccgcgc tcgcagaaca ggtgctcggc cgcatcgagg
     4141 atgcggttgc gggtttcctg cgcctcgtcc ttggtgcgcc gtaccactaa gcggttctcc
     4201 gttgcccgat gtgaaacatc gggcggccat aattcggtgc gataaattcg gtctttacat
     4261 acattcgcga ttgtatgtaa actgcctgct cttcgcaaca ggttgatacc cggaagggcc
     4321 gtgcgcccct gccgggccag ggaaaacaac gtcatgaaca cgctctccag catttcacgg
     4381 ggcggcctgc gcgctgcgct gcccgccatt ttctccatgg cgctcgcgct gcttgccgca
     4441 ggctgccagc ccgccggggg cccgcccggc ggattcccgc ctcccctcgt caccgtctcg
     4501 gtcgtcgaac ccagagatgt gccggtcacc tacgaatatg tcgcccagac cgcgggttac
     4561 cgcgagatcg aggtgcgcgc gcgcgtcacg ggcatcctgt tgaagcgcaa ttacaaggaa
     4621 ggtgcgacgg tccgccaggg cgagtcgctc ttcaccattg acccggcccc gttccaggtt
     4681 gcgctggcaa aagctgaagc cgacctgggg gtggcggaag cgcgcttcgc gcaggcgaag
     4741 cgcgagagcg gccggctgaa gcccgtgatc gatgccaggg cggcgagcca gaaggagctc
     4801 gacgacgccg tctccgccga gcaggtcgcc gacgcggagg tgaggagcgc ccgcgcgcgg
     4861 gtggcggaag cgaagctgaa cctcgattac acgcgggtgg agtcgccgat ctccggcatc
     4921 acgagccgcg ccgtcgtttc ggaaggggcg ctcgtgtccg gccccaacgt gctgctcacc
     4981 accgtgaccc agaccgatcc gatgtacgtc atcttcggca ttcccgaccg cgagttcctg
     5041 gcaatacggc gcgatgtcgc cgccggtcgc ctgaagatcc cggaaggcgg gcgcttcaag
     5101 gccacgatca agctctccga cggcagcttc tacgacaaga gcggcacgct caatttcgcc
     5161 gacgttcgcg tcaacggcca gaccggcacc agcgaggcgc gcgccgaagt cgccaactcg
     5221 ggcaatctgc tgcgcgcggg ggagttcgcc cgcgtggtcc tggagggagc aatgcgtccc
     5281 ggcgcgatgg tgataccgca gcgcgcagtg ctggagaacc ccaagggcaa gtacgtcttc
     5341 atcgtcaccg cggacggcaa gtcggaaccg cggccggtcg aggtcggcga ttggaccggc
     5401 gacggctgga tcatcatttc cggcctgaat cccggcgacc gcgtcgtcgt ggacggcgtg
     5461 atgaaactct cgctgatgcc gccagggtcc ccggtaaaga tcgccgatga cgcggcggcg
     5521 cccgaggaca agaagggccc gccggagaaa aaagggcccg ccgacaagaa gggggccgaa
     5581 ggcagcaagg cgccggcagt caaaaaagcg ccgccagaga agaaagtata aggcagccgc
     5641 acatgatttc acgcttcttc atcgacctgc cgatcttttc ggccgtgctc tcgattttcc
     5701 tcgtcatcgc ggggctcgca tcgatgcgcg tgctgccgat cgcgcagtat cccgagattg
     5761 cgccaccggt ggtcaccgtg cgcgcgatct atcccggcgc atcggcccag gtgctcgagc
     5821 agaccgtggc ggcgcccctc gagaaccaga ttaccggcgt cgagaacatg ctctacatga
     5881 gctcgacctc cacctcgagc ggcgtggtcg agatccaggt gaccttcgag atcggcgccg
     5941 acgtggatac cgcggcgctg aacgtcaaca accgcgtgaa gcaggtcgag ccgcgcctgc
     6001 cgctggaagt gcggcggcag ggcgtcacgg tcgaaaaggg ctcttcgtct ttcctccagg
     6061 tgatcgcttt cacctcgccc gacggcagcc gcgacgacct cttcatctcg aactacgtca
     6121 cgctgaacgt gctcgactcg ctgaagcgca tccccggcac gaccaacgtg cagatcttcg
     6181 gcgcgaagga ctacgcgatg cgcatctggg tgcgcccgga ccggctcgcg cagctgaagg
     6241 tctccccttc cgacctgatt cgcgcgatga acgagcagaa cgcccagttc gcggccggca
     6301 agatcggcca gtccccgacc agcggaccgc aggagctggt gtacacgatc accaccaagg
     6361 gccggctgac ggagcccgcg gaattcgaga acatcgtgat acgggcgaac cccgacggtt
     6421 ccatcctccg gatgaaggac gtcgcgcgcg tcgagctcgg ctccagggac tacgagttca
     6481 tgggccgcgt caacggcaag tcggcgacgc tcgtcggcat tttcctgcag ccgaacgcca
     6541 acgcgatcgc gaccggtgac cgcgtgaagc gggcgatggg cgatctggcc agccgtttcc
     6601 ccccggggct cgagcaccac gtcgtgtacg acacgacacg cttcgtggag gtttcgattc
     6661 gcgaggtcgt gatcacgctt gccgaggcga tggcgctggt gttcctggtg gtgttcctgt
     6721 tcctgcagaa ctggcgcgcc accctgatcc cgttcgccgc ggtgccggta tcgctgatcg
     6781 gcacttttgc cggcctggtc atgctcggct actcgatcaa cacgctcacc ctgttcggca
     6841 tggtgcttgc gatcggcatc gtggtggacg acgcgatcgt cgtgctggaa aacgtcgagc
     6901 gcatcatgca cgaggagcac ctgcaggcgc gcgaggcggc gatcaaggcg atgaaggagg
     6961 tcaccagccc gatcatcgc
//
LOCUS       DBNGSG010000274         3279 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1106233, whole genome shotgun sequence.
ACCESSION   DBNGSG010000274 DBNGSG010000000
VERSION     DBNGSG010000274.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1106233"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            255..992
                     /locus_tag="AC158Q_07130"
     CDS             255..992
                     /locus_tag="AC158Q_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007839084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3339586.1"
                     /translation="MHSTTALSGKHSPRQNHLLAALPAADYERLLPDLELVPLPLGWA
                     VYEAGGKLGYVYFPTSSIISLLYVMEDGASAEIAVTGHDGLVGIALFMGGETTPSRAV
                     VQSAGYGYRLKAAVLKKEFEQGGTLQHLALRYTQALITQMAQTAVCNRHHTVEQQLCR
                     WLLLSLDRLPSNELAMTQELIANMLGVRREGVTEAAGHLQAAGLIHYSRGHITVLDRP
                     KLEKRVCECYAVVKREMDRLLPDKTAH"
     gene            complement(1017..1595)
                     /locus_tag="AC158Q_07135"
     CDS             complement(1017..1595)
                     /locus_tag="AC158Q_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIR3339587.1"
                     /translation="MKTRPGWFVPNAALAGVMALVVAGCNKPREAAGTPPPVTTVGTA
                     IDDSVVTGRVKSALLADADVRSADFQVETRKGLVQLSGFVDNQAQIDRAMAVTRTVAG
                     VKDIENNASVKGAATTLGNKVDDGIVTTRVKAALLADASIRSRDISVVTRKGEVQLSG
                     FVDSLNQIDRAVEVARDVEGARSVSNEMSVKK"
     gene            1877..>3279
                     /locus_tag="AC158Q_07140"
     CDS             1877..>3279
                     /locus_tag="AC158Q_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase family protein"
                     /protein_id="HIR3339588.1"
                     /translation="MIPGSDRIRETLRRLAARLRGRHTALQHAGDEPPLRAALFSADQ
                     MERHGKVLAGMHRLGPGRAPDQLLARLAENEDILLHACGLLSATVKANRRITPAGEWL
                     LDNFYLIEEQVRTAKRHLPKRYSGELPRLAQGPSAGLPRVYDIALEAISHGDGRVDPE
                     SLDRFVAAYQKVTPLKLGELWAIPIMLRLALIENLRRVAARIVAGAVNRELADEWADR
                     MMEVAETDPKSLILVIADMARSTPPMVSPFVAEFARRLQGQSPALALPLTWIEQRLSE
                     ESATIEQLVQAETQSQAASQVSISNSIGSLRFLGAMDWRRFVETMSVVEQKLLEDPGG
                     VYGAMDFATRDSYRHAVEKIARKSILSEGDVARNAIRLAHDGAAGAPGTGGAAREAHV
                     GYYLVDKGLPQLEQSVQARWSAIETACRTSRRFPLALYLGAITALTAIFTWGLLAHAR
                     IGGLSGGPSDGMSDAMSG"
BASE COUNT          582 a         1094 c         1079 g          524 t
ORIGIN      
        1 cccccccccc cccccaaacg ccggtagcgg aggcccgcca cgaataaagc ccccggcatc
       61 aactgccggg ggccggaacc cgccaattca taatgaggga gatcattggc ggggaggagg
      121 aggctgaatt gttcacgtcc gctccccgcc tctctgtgcg ccagcgtaca gagtgggtag
      181 aatgcatttg gtataaagcc cgggcgaaac tcgggttctc gagtctgtcg ctccctccat
      241 cagacgttgc gcccatgcac agcacaaccg ccctatccgg caagcacagc ccgaggcaga
      301 atcatcttct ggccgctctg cccgcggcgg attacgagcg cctgctgccc gacctggaac
      361 tcgtgccgtt gccgctgggc tgggcggtct acgaagccgg cggcaagctg ggctacgtgt
      421 acttccccac cagcagcatc atttccctgc tctacgtcat ggaagacgga gcgtcggcgg
      481 aaatcgcggt caccggccac gatggcctgg tcggcatcgc gctgttcatg ggcggcgaaa
      541 ccacgccgag ccgggcggtg gtgcaaagcg ccggctacgg ctaccggctc aaggcggcgg
      601 tactgaaaaa ggaattcgag cagggcggca cgctgcagca cctggcgctg cgctacacgc
      661 aggcgctgat cacgcagatg gcgcagaccg cggtctgcaa ccggcaccat acagtggagc
      721 agcaactgtg ccgctggctc ctgctgagcc tggaccggct gccctcgaac gagctggcca
      781 tgacccagga actgatcgcc aacatgctgg gggtgcgccg cgagggcgtc acggaggcgg
      841 ccgggcacct gcaggcggcc gggttgatcc attacagccg cggccacatc accgtgctcg
      901 accgcccgaa gctggagaag cgggtgtgcg agtgctacgc ggtggtgaaa cgggaaatgg
      961 accggctgct tcccgacaag acagcacact gattaccgct gtcccgaaat tgcgtcttac
     1021 ttcttgacgc tcatctcgtt gctgacgctg cgcgcgccct cgacgtcgcg ggccacttcc
     1081 acggcgcggt cgatctggtt caggctatcg acgaagccgc tcaactggac ctcgcccttg
     1141 cgcgtcacga cggagatgtc ccggctcctg atgctcgcat cggcaagcag ggcggccttg
     1201 acccgggtcg taacgatccc gtcatcgacc ttgttgccca gcgtcgtcgc cgcgcctttc
     1261 acgctcgcgt tgttttcgat gtccttgaca cccgcaaccg tgcgcgtgac ggccatggcg
     1321 cgatcgatct gcgcctggtt gtcgacgaag ccgctcaact ggaccagtcc cttgcgggtt
     1381 tcgacctgga aatccgcgct cctgacgtcc gcgtcggcaa gcaatgctga tttgaccctg
     1441 cctgtaacca cgctgtcgtc aatcgccgtc cccactgtcg tcaccggcgg cggcgtgccg
     1501 gccgcttccc ggggcttgtt gcagccggcg acaaccaatg ccatcacgcc cgccagcgct
     1561 gcattcggga caaaccatcc ggggcgggtt ttcatgtgaa attcatttcc agcatttatc
     1621 cgtgggccag gcggcaaccg tgaatgcgac cctcctccct ttcgccgcgt ttctctgtgc
     1681 gccagcgcac gtaagaatcg ggccattcct gtcaaaattc gcgtagaacc cgccaggcac
     1741 atcgaagcgg gtgacggcgt ccggccgaat caggtcgcgg ccggggtcgt tccgcgggcg
     1801 gcccgtcgtc ctcaatcatc ctcgccacgg ccggcgtgaa ggcatcgcct cgtcacggcg
     1861 gagaaggaaa cctgaagtga taccgggcag cgacagaatc cgcgagaccc tgcgccgact
     1921 ggcggcgcgc ctgcgcggca ggcacacggc gctccagcac gccggcgatg agccgccgtt
     1981 gcgggcggcg ctgttcagcg ccgaccagat ggagcggcac ggcaaggtcc tggcgggcat
     2041 gcaccgcctg ggtcccgggc gcgcgcccga ccagctgctc gcgcgcctgg ccgagaacga
     2101 ggacattctg ctgcacgcct gcggcctgtt gagcgccacg gtgaaggcaa accgccggat
     2161 tacgccggcc ggggaatggc tgctcgacaa tttctacctg atcgaagagc aggtgcgcac
     2221 ggccaagcgc cacctgccga agcgctacag cggcgaactg ccgcgcctcg cgcaaggccc
     2281 gtcggccggg ttgccgcgtg tatacgacat tgccctggaa gcgatctcgc acggcgacgg
     2341 gcgcgtcgac ccggaaagcc tcgaccgctt cgtggcggcg tatcagaaag tcacccccct
     2401 caagctgggt gaactatggg cgatccccat catgctgcgc ctggcgctga tcgagaatct
     2461 gcgccgcgtc gcggcccgca tcgtcgccgg cgcggtcaac cgcgagctcg ccgacgaatg
     2521 ggccgaccgg atgatggagg tcgccgagac cgacccgaag agcctgatac tcgtgatcgc
     2581 ggacatggcg cgctcgacgc cgccgatggt gagtccgttc gtcgctgaat tcgcgcgccg
     2641 tttgcagggc cagagcccgg cgctggcgct gccgctgacc tggatcgagc aacgattgtc
     2701 cgaagagagc gcgacgatag agcaactggt ccaggcggag acccaaagcc aggccgcgag
     2761 ccaggtctcc atcagcaaca gcatcggcag cctgcgcttc ctgggggcaa tggactggcg
     2821 caggttcgtc gaaacgatga gcgtcgtcga gcagaagctg ctggaagacc cgggcggagt
     2881 ctacggcgcg atggatttcg ccacgcgcga cagctaccgg cacgcggtcg agaaaatagc
     2941 gagaaaaagc attctgtcgg aaggcgacgt cgcgcgcaac gcgatccggc tggcgcacga
     3001 tggcgcagcg ggagcgcccg gcacgggcgg cgctgcgcgc gaggcgcatg tcggctacta
     3061 cctggtggac aaggggctgc cgcaactcga gcaatcggtg caggcgcgct ggtccgcgat
     3121 cgaaaccgcg tgcaggacga gccgccggtt tccgctggcc ctgtacctgg gggccatcac
     3181 ggccctgacc gcgattttca cgtggggctt gctggcccat gcccggatcg gaggactgag
     3241 cggcggaccg agtgacggaa tgagtgacgc aatgagcgg
//
LOCUS       DBNGSG010000275         6684 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1275500, whole genome shotgun sequence.
ACCESSION   DBNGSG010000275 DBNGSG010000000
VERSION     DBNGSG010000275.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6684)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6684)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6684
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1275500"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..599
                     /locus_tag="AC158Q_07145"
     CDS             147..599
                     /locus_tag="AC158Q_07145"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3339589.1"
                     /translation="MTIGAICDRRIATASRATSVFVAAKSMCAFDERVLVVTDRKDGR
                     LSAVGIVTEHEFVSRIAALRADPAALTLQDIMRHDIAFVGEADSLHDTVRWMRRNRFR
                     EAVVLGEAGGLIGIVTLEQLCASMAEESAASVSPGAAGPVELGDNAIH"
     gene            650..2140
                     /gene="pap"
                     /locus_tag="AC158Q_07150"
     CDS             650..2140
                     /gene="pap"
                     /locus_tag="AC158Q_07150"
                     /EC_number="2.7.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043751 - polyphosphate:AMP
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate:AMP phosphotransferase"
                     /protein_id="HIR3339590.1"
                     /translation="MFESAELGHKLSKGQYKRIEPQLRADLLDAQYDLFKNGKFPVIV
                     LISGVRGAGKGETVNLLNEWMDPRHIHTHAFDDPSDEERDRPPMWRYWRALPPRGRIG
                     ILFGSWYTAPIIDRVFKRTTSKDLMRSVIEINQFERMLADEKALILKFWFHLSKKRQK
                     KRLKELESDPETRWRVTRRDWDFLKHYDRFYRISESTLRDTSTADAPWIVVEGSDPEY
                     RAVTVGKTLLEAMRKRLQGPPERRVAKSQAAPIIRPVDKLHVLAKMDFSLKLEKPKYE
                     ERLEKLQRKLALATRHPKFGSRNVVCLFEGMDAAGKGGAIRRITAALDARLYHTIPVA
                     APSDEERAQPYLWRFWRHVPRKGRFTLYDRSWYGRVLVERIEGFCSEADWMRAYSEIN
                     DFEQQLARNGAIIVKYWLQISKEEQFRRFKEREKTRFKRFKITAEDWRNREKWDAYQV
                     AASDMIERTSTAHAPWVLVEANDKYHARIKMLSTLVDAIEGALGKR"
     gene            2163..3731
                     /locus_tag="AC158Q_07155"
     CDS             2163..3731
                     /locus_tag="AC158Q_07155"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR3339591.1"
                     /translation="MGTFATGAWQLFWREELPYLLVAAALVALILARVPRMERGALTH
                     TLLFLALALAGELCGALLEAGGAARSGAIVRDAAIVATGLAVIRLAGLALFRAALPAV
                     GITPPRIAEDIVLVITYVAWGLLRLRLAGMDLASLVTTSAVITAVVAFAMQDTLGNVL
                     GGLFLEMDGSISIGDWIRLDDLSGRVTEIRWRHTAIRTRNGELVIVPNSALMKSRFAV
                     IGNPDEEEVRWRRWIWFDVSFDVSPATVLAAAGQALAGAEIGNVAREPAPSCVLMDFG
                     PSSCRYALRYWLRDPQPDDVTDTAVRAHLWAALQRAGIAFAFPVSIVHRIKEGEAREA
                     ALHAREIEARMAALRGVEIFSYLDEQELRSLADHLVPAPFAAGDIMTRQGAIAHWLYI
                     LTEGEAEVWVDTPGAPRRRVAVIGSGNVFGEMGMMTGEPRTATVTARSDVRCYRLDKA
                     GFEGILHSRPAIAEEISRVLEARASGLRDIVEAARAEAGAARARPGKMLERIRAFFGL
                     DRPPTAGAHVAPRR"
     gene            complement(3854..5707)
                     /locus_tag="AC158Q_07160"
     CDS             complement(3854..5707)
                     /locus_tag="AC158Q_07160"
                     /inference="COORDINATES: protein motif:HMM:NF012449.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HIR3339592.1"
                     /translation="MTVPTTNERADALRASLLGLMTAIAEEEEAQRAHIDAVLPKHRL
                     SAANLAHYLGLRKQDIHRLQLDLSAVGLSSLGRSEGHVRDTLLRLCAWLAGERGDAMT
                     GESEERLDWAKAERILHANTRALFGPRPPDRHVYIMVTAPEAEEVTGAWADEILKAGA
                     NLLRINGAHESPQEWTAIVNVFKDRAFALGKTARIVVDLPGPKLRTEILQKDAAVLRL
                     RRVKDRLGRTVAPTEILFVAEFQDKPQIPVPAHWPPQLHAGDLVSLTDAGGRYRDIVV
                     AGPAGGGVAARCDRSLYVMPGLPLVWRRGDVVMEEGRVGPLPGLPGELRLSVGDAFLV
                     NASGQSDDPDAPGALAFPDAGLLSQVRPGERVVLDDGRIVAVAEAERPGGLLCRVQQA
                     LKAPTRLRSGKGIAFPDSELVLGQLGPQDELALQFALDHADGVNISFVNSAEDVAVVS
                     ERLRESGKAGFGMTLKLETRAAMRNLPAILFEALKYDPVGIMIARGDLAVELSFERLA
                     EMQEELLWFGEACHIPVIWATQVLDTVAHSGLPTRAEITDAAMSMRAECVMLNKGPYL
                     ALATRMLADIIRKMETHQYKKRSLYRQLAVAKSTRSAGKAELPVPAAAKRS"
     gene            5924..6589
                     /locus_tag="AC158Q_07165"
     CDS             5924..6589
                     /locus_tag="AC158Q_07165"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HIR3339593.1"
                     /translation="METGQFRNRQDAALRLARRLKPLELKSPVVLAVPRGGVPIGAVL
                     ARELGGELDVVLARKLRAPYQREYAIGSVGEDGTVYLDPDARRVEGVTEAYLQAEREL
                     QIAEIAQRRQRFRSAVPALDVAGRAVIVTDDGIATGSTMLAALHVLGALKPREVIVAV
                     PVAPPDTLERFRAKCDRVVCLLAPDYLGAISQFYEDFTQVEDEEVVRLLRESLAAQVA
                     SKP"
BASE COUNT         1048 a         2206 c         2368 g         1062 t
ORIGIN      
        1 gatctgaatc aatccgtccc cccctcgccc ggcgcggtcg tcgccgacct cggccatcgc
       61 catcgccgcg attgatccaa atcaataaag tttttcccgc agcgataaga atggagcagg
      121 cgaattccgg ttcacggagg tgcagtatga cgataggggc gatatgcgac cggcgcattg
      181 caacggcctc gagggccacc agcgttttcg tggcggcgaa atccatgtgc gcattcgacg
      241 agcgcgtcct ggtggtcacc gacaggaagg atggacggct gtccgcggtg gggatcgtga
      301 cggagcacga gttcgtgtcc cggatcgccg cgctgcgggc cgaccccgcc gcgctcacgc
      361 tgcaggacat catgcgccac gacatcgcgt tcgtcggtga agccgactcc ctgcacgaca
      421 ccgtgcgctg gatgcgccgc aaccggttca gggaggccgt cgtgctgggc gaggcgggcg
      481 gcctcatagg catcgtgacc ctcgagcagc tgtgcgccag catggcggag gaatccgctg
      541 cttcggtgtc gccgggcgcc gccggcccgg tcgagctcgg cgataacgcg attcattaga
      601 cgcggccgcc cgagacgcgg gctgcgcgca gggaagacag gggaacccca tgttcgaatc
      661 cgcggagctg ggccacaagc tgtccaaggg gcaatacaag cgcatcgagc cccagctgcg
      721 cgccgacctg ctcgacgcgc agtacgatct tttcaagaac ggaaaatttc cggtcatcgt
      781 gctcatttcg ggcgtgcgcg gcgccggcaa gggggagacc gtcaatttgc tgaacgagtg
      841 gatggatccc cgtcatatcc atacccacgc cttcgacgac ccgtcagacg aggagcggga
      901 tcgcccgccc atgtggcgct actggcgggc gctgccgccg cgcgggcgca tcggcatcct
      961 gtttggctcc tggtacacgg cgcctatcat cgaccgggtg ttcaagcgca ccacctcgaa
     1021 ggacctgatg cggtcggtca tcgaaatcaa ccagttcgag cgcatgctgg ccgacgagaa
     1081 ggcgctcatc ctgaagttct ggttccacct gtcgaagaag cgccagaaga agcggctgaa
     1141 ggagctcgaa tccgacccgg aaacccgctg gcgcgtcacc cggcgcgact gggacttcct
     1201 gaagcactac gaccgcttct accgaatcag cgagagcacg ctgcgggaca ccagcaccgc
     1261 ggatgcgcca tggatcgtgg tcgagggctc ggaccccgag taccgcgcgg tgacggtggg
     1321 caagacgctg ctcgaagcga tgcgcaagcg cctgcagggg ccgcccgagc ggcgggtcgc
     1381 caagagccag gccgcgccga tcatccggcc ggtcgacaag ctgcacgtgc tggcgaagat
     1441 ggatttcagc ctgaagctgg agaagccgaa gtacgaggag cgcctcgaga agctgcaacg
     1501 caagctggcg ctggcgacgc ggcacccgaa gttcggcagc cgcaacgtcg tgtgcctgtt
     1561 cgagggcatg gacgccgccg gcaagggcgg cgcgatccgc cgcatcaccg ccgcgctcga
     1621 tgcgcggctc tatcacacca tccccgtcgc ggcgccgtcc gatgaggagc gggcgcagcc
     1681 ctacctctgg cggttctggc ggcatgtccc gcgcaaggga cgcttcaccc tgtacgaccg
     1741 cagctggtac gggcgcgtgc tggtggagcg gatcgagggc ttctgctcgg aggcggactg
     1801 gatgcgggcg tactccgaga tcaacgactt cgagcagcag ctcgcgcgca acggggcgat
     1861 catcgtcaag tactggctgc agatctccaa ggaagagcag ttccggcgct tcaaggagcg
     1921 ggagaagacg cgcttcaagc gcttcaagat cacggccgag gactggcgca accgtgagaa
     1981 atgggacgcc taccaggtgg cggcatcgga catgatcgag cgcaccagca cggcgcacgc
     2041 gccgtgggtg ctggtcgagg cgaacgacaa gtaccacgcg cgcatcaaga tgctgtccac
     2101 cctcgtggat gcaatcgagg gcgcgctggg caagcgctga ggcgaggcgc gggaagccat
     2161 ccatgggtac cttcgcaacc ggggcgtggc agctcttctg gcgcgaggag ctgccatacc
     2221 tgctcgtcgc tgccgcgctc gttgcgctga tcctcgcgcg cgtgccgagg atggagcgcg
     2281 gcgcgctgac tcacacgctg ctgttcctcg cgctcgcgct ggcgggggag ctttgtggcg
     2341 cgctgctcga ggccggcggg gcggcgcggt ccggggcgat cgtgcgcgat gccgccatcg
     2401 tcgccacggg gctcgccgtc atccgcctcg ccgggcttgc gctgttccgg gccgcattgc
     2461 cggccgtggg catcaccccg ccgaggattg ccgaggacat cgttctggtg atcacttacg
     2521 tcgcctgggg cctgctgcgg ctgcgcctcg ccgggatgga cctggcgagt ctggtgacga
     2581 cttcggcggt gatcacggcg gtggtggcat ttgcgatgca ggacacgctt ggcaacgtgc
     2641 tgggcgggct gttcctcgag atggacggct cgatctccat cggtgactgg atccggctcg
     2701 acgacctgtc aggccgcgtc acggaaatcc gctggcgcca caccgcgata cgcacgcgca
     2761 acggcgagct cgtgatcgtg ccgaacagcg ctctcatgaa atcgcgcttc gccgtcatcg
     2821 gcaacccgga cgaggaagag gtgcggtggc ggcgctggat ctggttcgac gtcagcttcg
     2881 atgtgtcacc ggcgacggtg ctggcggccg cgggccaggc gctcgccggc gccgaaatcg
     2941 gcaacgtcgc gcgcgagccg gcgccgagct gcgtgctgat ggatttcggt cccagcagtt
     3001 gccgctacgc cctgcgctac tggttgcgcg atccccagcc tgacgatgtc accgataccg
     3061 ccgtgcgcgc ccacctgtgg gccgctctgc agcgcgcggg catcgcgttc gccttcccgg
     3121 tgtctatcgt gcaccggatc aaggagggcg aggcacgcga ggcggcactg cacgcgcgcg
     3181 aaattgaagc gcggatggcc gcgctgcgcg gcgttgagat tttttcgtat ctggacgaac
     3241 aggaattgcg ttcgctggcg gaccatctcg tgccggcgcc atttgccgcg ggtgacatca
     3301 tgacccggca gggcgcgatc gcgcactggt tatacatcct gaccgagggg gaggccgagg
     3361 tgtgggtgga tacaccgggc gcgccccggc gccgggtcgc ggtgatcggt tccggcaacg
     3421 tcttcggcga gatggggatg atgaccggag agccgcgcac cgcgacggtg accgccagga
     3481 gcgacgtgcg atgctatcgc ctggacaagg cgggattcga gggcatcctc cattcgcgcc
     3541 cggccatcgc ggaggagatt tcccgcgtgc tggaggcgcg cgcaagcggt ctgcgagaca
     3601 tcgtcgaggc ggcgcgggcg gaggcaggcg cggcacgagc gcggccgggc aaaatgctcg
     3661 agcgcatccg cgcgttcttc gggctcgaca ggccgccgac ggccggcgcg cacgttgcgc
     3721 cgcggcgctg actccggcag tgaggaagaa cgtgacgcag tgacgcagcg atgcagtgac
     3781 gtgctcccct cttcgggaag gaggggaatg ttttctcgta atgactgacg actaacgagt
     3841 gtcgcgtctc ccgtcacgac ctcttggccg ccgccgggac tggcagctcg gctttgccgg
     3901 cagagcgcgt gctcttcgcc acggccagct gccggtagag cgaccgcttc ttgtactggt
     3961 gcgtttccat cttgcggatg atatcggcga gcatgcgcgt cgccagggcg aggtagggcc
     4021 ccttgttcag catcacgcac tcggcgcgca tggacatcgc cgcgtcggtg atctcggccc
     4081 gcgtcggcag gccggaatgg gccacggtgt cgagcacctg cgtcgcccag atcaccggga
     4141 tgtggcaggc ctcgccgaac cacagcagct cttcctgcat ctcggcgagg cgctcgaagg
     4201 agagctccac cgcgaggtcg ccgcgcgcga tcatgatccc gacgggatcg tacttgagcg
     4261 cctcgaacag gatggcgggc aggttgcgca tggcggcgcg cgtctccagc ttgagggtca
     4321 tgccgaaccc cgccttcccg gattcccgca ggcgctcgct gaccacggcc acgtcttcgg
     4381 cggaattgac gaaggagatg ttgaccccgt cggcatgatc gagcgcgaat tgcagcgcga
     4441 gttcgtcctg cgggccgagc tgcccgagca ccagctcgct gtcggggaac gcgatgccct
     4501 tgccggagcg caggcgggtc ggcgccttga gcgcctgctg cacgcggcac agcaggccgc
     4561 cggggcgctc ggcttccgcg accgcgacga tgcgcccgtc gtcgagaacc acccgctctc
     4621 ccggccgcac ctgcgacagg agccccgcgt ccgggaaggc cagtgcgccc ggcgcgtccg
     4681 ggtcgtcgct ctgcccgctc gcgttcacca ggaaggcgtc accgacgctc aatcgcagct
     4741 ccccggggag gccggggagg gggccgacgc ggccttcctc catcacgacg tcgccgcgcc
     4801 gccagacgag ggggagcccg ggcatcacgt agagcgagcg gtcgcagcgc gcggccacgc
     4861 cgccgccggc cggcccggcg acgacgatgt cgcggtagcg cccgccggcg tcggtcaggc
     4921 tcaccaggtc gccggcatgc aattgcggcg gccagtgcgc cggcacgggg atctgcggct
     4981 tgtcctggaa ttcggcgacg aacaggattt cagtcggcgc cacggtccgc cccagccggt
     5041 ccttgacgcg ccgcaaccgc agcacggccg cgtccttctg caggatctcg gtgcgcagct
     5101 tcgggccggg gaggtcgacg acgatgcgcg cggtcttgcc gagcgcgaag gcgcggtcct
     5161 tgaagacgtt cacgatcgcc gtccactcct gcggcgattc gtgcgccccg ttgatccgca
     5221 gcaggttggc gcccgccttg aggatctcgt ccgcccaggc gccggtgacc tcttccgctt
     5281 ccggggccgt caccatgatg tagacgtggc ggtccggcgg ccgcggcccg aacagcgcgc
     5341 gggtgttggc atggagaatg cgctcggctt tcgcccagtc cagccgctct tccgattctc
     5401 ccgtcatcgc gtcgccgcgc tcgcccgcca gccacgcgca caggcgcagc agcgtgtcgc
     5461 ggacgtggcc ttccgaccgg cccagcgagg agaggccgac cgccgagaga tcgagctgca
     5521 ggcggtggat gtcctgcttg cgcagcccga ggtagtgcgc gaggttcgcg gcgctcagcc
     5581 ggtgcttcgg caggaccgcg tcgatgtggg cgcgctgggc ttcctcctcc tcggcgatcg
     5641 ccgtcatgag tccgagcagg ctggcacgca gcgcgtcggc gcgttcgttg gttgttggga
     5701 ccgtcatctc cccgccttgg gcccggttcg ctcgacccat gggaatcatg agttacggcc
     5761 cctggtcgaa ccttgatctg gatcagcccc ttccgggatg cttgcgatgg gcagaccggg
     5821 cggcgagcgc cctgcttgat atgcatcaag ccggtccagc cgggccaggt tcaggataca
     5881 agctggcggg gcgtattgca gtcgatccga gcggggtgtt gccgtggaaa cgggccagtt
     5941 caggaaccgg caggatgcgg cgctgcggct cgcccggcgc ctgaagccgc tagaactgaa
     6001 gtcgccggtc gtgctggccg tgccgcgtgg gggcgttccc atcggcgccg ttctcgcccg
     6061 cgagctgggc ggggagctcg acgtcgtgct cgcgcgcaaa ttgcgcgcgc cctatcagcg
     6121 cgaatacgcg atcggttccg tcggggagga tggcacggtc tacctggatc ccgatgcgag
     6181 gcgcgtcgag ggcgtgacgg aagcctacct ccaggcggag cgcgaactgc agatcgcgga
     6241 aatcgcgcag cgccgccagc ggtttcgctc cgccgttccg gcgctggatg tcgccgggcg
     6301 agcggtcatc gtgaccgacg acggcattgc caccggctcg accatgctgg cggcgctgca
     6361 cgtcctcggc gccctgaaac cgcgcgaagt catcgtggcg gtgcccgtcg cgccaccgga
     6421 cacgctcgag cgctttcggg cgaaatgcga tcgcgtggtc tgcctgctcg cgccggacta
     6481 cctgggcgcg atcagccagt tctacgagga tttcacgcag gtcgaggatg aggaggtggt
     6541 caggctgctg cgcgagtccc tcgccgcgca ggtcgcgtcg aagccatgaa gatcggcgtc
     6601 ccgagagaaa ccaaggtcca ggaatatcgc gtgggcctca ctccggagag cgcgggcacg
     6661 ctgaccgcgc gcggccaccg cgtc
//
LOCUS       DBNGSG010000276         2705 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3653361, whole genome shotgun sequence.
ACCESSION   DBNGSG010000276 DBNGSG010000000
VERSION     DBNGSG010000276.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3653361"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..375
                     /gene="glnK"
                     /locus_tag="AC158Q_07170"
     CDS             37..375
                     /gene="glnK"
                     /locus_tag="AC158Q_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HIR3339594.1"
                     /translation="MKLVTAIIKPFKLDEVREALSAIGVTGITVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKVEAAIKSEMLEQVIEAIEKSANTGKIGDGKVFVFELEQVIRI
                     RTGETGADAL"
     gene            386..1876
                     /locus_tag="AC158Q_07175"
     CDS             386..1876
                     /locus_tag="AC158Q_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIR3339595.1"
                     /translation="MKKILTILALFGALSFAVMPAAAQDKPAEAPAAAPAAAAPAAAP
                     AAPAATPAPAPTPNKGDIAWLLTCTALVLMMSVPALALFYGGMVRAKNMLSVLMQVFV
                     TFSLITVLWCIYGYSLAFTEGNAFFGGFDRLFLGGTFDSAKGEFAMAATFSKGVVVPE
                     LAFVAFQATFAAITCCLIVGAFAERVKFSAVLIFMVLWFTFSYTPIAHMVWYWMGPDA
                     YTSMKLVDELNGKAGLLWQWGALDFAGGTVVHINAGVAGLVGAYLVGKRIGFGKEAMT
                     PHNLPMTMIGAALLWVGWFGFNAGSALEAGTSATLAFINTFLATACAVLAWTFGEWLF
                     KGKPSMLGAASGAVAGLVAITPAAGNVGIPGAFVIGTAAGFVCLWAVNQLKRWLGADD
                     SLDVFGVHAVGGILGALLTGVFNAPDLGGPGFVNNWFEMKPGFGSIADQVWIQAKAVG
                     VTVIWTAVVALISFKIADLIVGLRVTEEEEREGLDVNSHGETAYHT"
     gene            2040..>2705
                     /gene="gshA"
                     /locus_tag="AC158Q_07180"
     CDS             2040..>2705
                     /gene="gshA"
                     /locus_tag="AC158Q_07180"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HIR3339596.1"
                     /translation="MTVPRLTTALAGPLPDLERQLLKSQAEAEHWLRGQWQDHATPFY
                     CSVDLRNSGFKLAPVDTNLFPGGFNNLNPEFRPLCVQAAMAAIEKACPEARGMVLIPE
                     NHTRNQYYLQNVAVLQTILRQAGISVRIGSLLPEITAPTEITLENGAKLKLEPLERRG
                     NRLAVNGFDPCVVLLNNDLSAGTPGILKNLEQAVLPPLHAGWTVRRKSNHFAAYRQVA
                     EEFA"
BASE COUNT          480 a          877 c          856 g          492 t
ORIGIN      
        1 cctgcatggc cggtcccatt tccacgggga atcccaatga aactcgtcac tgcaatcatc
       61 aagccgttca agctcgacga ggtgcgtgaa gcgctgtccg ccatcggcgt gaccggcatc
      121 accgtcaccg aggtcaaggg attcgggcgg caaaaggggc ataccgaact ctaccgcggg
      181 gccgaatacg tggtggactt cctgccgaag gtcaaggtcg aggccgcgat caagagcgaa
      241 atgctcgagc aggtgatcga ggcgatcgag aaatccgcca ataccggcaa gatcggcgac
      301 ggcaaagtct tcgtcttcga gctcgaacag gtcatccgca tccgcaccgg cgagaccggc
      361 gccgatgcgt tatagggaga atgcaatgaa gaaaatactg actatcctcg cgctgttcgg
      421 cgcattgagc tttgccgtca tgccggcagc ggcccaagac aagccggccg aggcgccggc
      481 tgccgcgccc gcggcggctg cgcctgctgc ggcacctgcc gcgccggcgg caactccggc
      541 gcccgcaccg acgcccaaca agggcgacat cgcgtggctg ctaacctgca ccgcgctggt
      601 cctgatgatg agcgtgccag ccctcgcgct gttctacggc ggcatggtgc gcgccaagaa
      661 catgctctcg gtgctgatgc aggtgttcgt caccttctcg ctgatcacgg tgctgtggtg
      721 catctacggc tacagtctcg cattcaccga gggcaacgcc ttcttcggcg gcttcgatcg
      781 cctcttcctg ggcggcacat tcgattccgc caagggcgaa ttcgccatgg ccgcgacttt
      841 cagcaagggt gtcgtggtac ccgagctggc ctttgtcgcg tttcaggcga ctttcgctgc
      901 gatcacctgc tgcctgatcg tcggcgcctt tgccgagcgc gtgaaattct ccgcggtgct
      961 gattttcatg gtgctgtggt tcacgttcag ctacacgccg atcgcgcaca tggtgtggta
     1021 ctggatgggg cccgacgcgt acacgagcat gaaactggtg gacgagttga acggcaaggc
     1081 cggtctcctg tggcaatggg gcgcgctcga tttcgcaggc ggaacggtcg tgcacatcaa
     1141 cgccggggtc gccggcctgg tgggcgctta cctcgtcggc aagcgcatcg gcttcggcaa
     1201 ggaagccatg acgccacaca acctgccgat gaccatgatc ggcgctgcgt tgctgtgggt
     1261 gggctggttc ggcttcaacg cgggctccgc gctcgaggcc ggcacgtcgg ctactctcgc
     1321 cttcatcaac accttcctcg ccaccgcctg cgcagttctg gcatggacct tcggcgagtg
     1381 gctgttcaag ggcaagcctt ccatgctggg cgccgcttcc ggcgccgtcg cgggtctggt
     1441 cgcgatcaca ccggctgccg gcaacgtcgg cattcccggt gcgttcgtga tcggcaccgc
     1501 agccggcttc gtctgcctgt gggcggtcaa ccagctgaag cgctggctcg gcgcggacga
     1561 ttctctcgac gtgttcggcg tgcacgccgt cggcgggata ctcggtgcgc tgctgaccgg
     1621 tgtattcaac gcgccggacc tcggcggacc cgggttcgtc aacaactggt tcgagatgaa
     1681 gcccggattc ggcagcatcg ccgaccaggt ctggatccag gccaaggccg tcggtgtgac
     1741 cgtaatctgg accgccgtcg tagcgctcat ctccttcaaa atcgcagacc tcatcgtggg
     1801 gctgcgcgtg acggaagaag aggaacgcga aggcctcgac gtgaactccc acggcgagac
     1861 ggcctatcac acttgagatt ttgcttcagg aagcatccgg acgggcgcct tcgggcgccc
     1921 gttttcctgt accgagatcc tggcctgaga gccttgtttt gacacgataa aaccccgccc
     1981 gggaaacggt cttttgcctt ccgttacaat aggcgcctcc gaggaagagg taaaaaaaca
     2041 tgaccgttcc gcgattgacc accgcgctcg ccggaccgtt gcccgatctc gagcgccagc
     2101 tgctcaagtc ccaggccgag gccgagcact ggctgcgcgg ccagtggcag gaccacgcta
     2161 cgcccttcta ttgctcggtg gacctgagga acagcggttt caagctcgcg ccggtggaca
     2221 ccaatctttt ccccggcggc ttcaacaacc tcaacccgga gttccgcccg ctgtgcgtgc
     2281 aggcggcgat ggcggcgatc gaaaaagcct gccccgaagc ccgcgggatg gtgctgatcc
     2341 ccgagaatca cacgcgcaac cagtactacc tgcagaacgt cgcggtgctg caaacgatcc
     2401 tgcggcaggc gggcatcagc gtgcgcatcg gcagcctgtt gcccgagatc acggcgccga
     2461 ccgagatcac cctcgaaaac ggcgcgaagc tgaaactcga gccgctcgag cgccgcggca
     2521 accggctggc ggtgaacggg ttcgatccgt gcgtggtgct gctcaacaac gacttgtccg
     2581 ccggcacgcc cgggatactg aagaacctcg agcaggcggt gctgccgccg ctgcacgccg
     2641 gctggaccgt gcgccgcaaa tcgaaccatt tcgcggccta ccgccaggtg gccgaggagt
     2701 tcgcc
//
LOCUS       DBNGSG010000277         5175 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1786026, whole genome shotgun sequence.
ACCESSION   DBNGSG010000277 DBNGSG010000000
VERSION     DBNGSG010000277.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5175)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5175)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5175
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1786026"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="AC158Q_07185"
     CDS             <1..203
                     /locus_tag="AC158Q_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR3339597.1"
                     /translation="DYMYYLERACQTQVTAMAGGAKLRIPAPAVAAKTADQFRALPYK
                     PKRPEWKALLRMLDKTDPTYKN"
     gene            complement(462..1352)
                     /locus_tag="AC158Q_07190"
     CDS             complement(462..1352)
                     /locus_tag="AC158Q_07190"
                     /inference="COORDINATES: protein motif:HMM:NF020650.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3339598.1"
                     /translation="MEKIKIQFTLFSAFYSPLISAMSGGFLKAEGLDPEWSISPPGVS
                     ALAALTDGTAHVAQSALSQGFTTLNKGETPATVHFAQVNEMDGFFLTGRGADPAFTWK
                     KLEGAEVVMFEGGQPLAMFQYACHKAGIDFGRIKPICPGGAAQIDRAFRDGKGQYVHQ
                     QGPYPQQLQADGVGHIVAQVGPQIGPCGFSSLAATREWLNTGMAKTFIRAYRRTRAYM
                     IATPAAAIARAQKPFFPDIGESVLADCIATYQKLGCWTPHVEITRAAYEKTLDVYEYN
                     GLLKQRYRYEQVCAAPPAVD"
     gene            1501..1803
                     /locus_tag="AC158Q_07195"
     CDS             1501..1803
                     /locus_tag="AC158Q_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HIR3339599.1"
                     /translation="MRVIALSTLRAFWTGKAAYADAREPTLAWYRHVLQADWRSPAEV
                     KREFGSASILRDGRVVFNIAGNKYRVVTWINYPYRVVYIRFIGTHAQYDRIDAQSI"
     gene            1816..2190
                     /locus_tag="AC158Q_07200"
     CDS             1816..2190
                     /locus_tag="AC158Q_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3339600.1"
                     /translation="MEIRPIKTKADYRAALKEIEALMAAERDTPEGERLDVLVTLVEA
                     YERKHYRFDLPDPVEAIKFRMEQKGMTPKDLVPMIGRINRVYEILSRKRPLTLAMVQR
                     LHSGLGIPAESLIKQPDRRKAA"
     gene            2439..3395
                     /locus_tag="AC158Q_07205"
     CDS             2439..3395
                     /locus_tag="AC158Q_07205"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HIR3339601.1"
                     /translation="MKARFPVCMLAAGLAAALAAPAHAQNYPVKPIRIVTSPAGGGND
                     FPARLIARSLPGPLGQQIVVDNRATILIADIVAKSPPDGYTLLVTGSAHWIGPLLDKV
                     TYDPIRDFAPITLIDRAPSVLVVHPSMPLKSIKQLIALARARPGEMNFSVGGPGSSGY
                     LGAILFNHMAGVNIVSVPYKGTAPALAAVMSGEVHAMFGSAGSVAPHAASGRLRPLAV
                     GSAQRSRLAPGLPTVAEAGLPGFLSEALHGLFAPAGTPAAAIALLNREVGNYLRSPEA
                     QGIFLKAGIEPSPGAPEELTAIMKSEMTRIGAVLKATGTARR"
     gene            3563..4327
                     /locus_tag="AC158Q_07210"
     CDS             3563..4327
                     /locus_tag="AC158Q_07210"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3339602.1"
                     /translation="MGQLQGKIALVAGAGRNNGRAIALAFAREGADLVLVAREKQSAL
                     DEVAHECEKLGARVLPLLCDVSEHEQVSRMAQQALGHFGHIDVLMGVAGRRAHQDFWE
                     IGLEEWHKTFAVNLHSLFYLARAIVPSMMERRGGSIVALGGLASLTAQPQRAHVVASK
                     TGLYGLVRALALELGPYNIRANLIALGMIENVRANPEWYPGTQGGAYKDDELAGIALK
                     RAGKPQEVANVAVFLASDQSSFVTGDRIVCAGGKYL"
     gene            complement(4347..4982)
                     /locus_tag="AC158Q_07215"
     CDS             complement(4347..4982)
                     /locus_tag="AC158Q_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339603.1"
                     /translation="MEAILKGSAFWQWIGQVMTVFFLIGGIVCVAVGVSLIVNSQRTL
                     RFFGTMNRWVSLRSASRPLEIPRDTTQAVQKRRYWLGALFIAGGVYATYYLATRFSAG
                     AAIQLFNLAALPAALAAWIVDSARWILIAGNLVAIAIGVLLAFSPATLAALEAGGSRW
                     FSERRLLKGADTQNLSLDKWVAAYPRRAGVIIVVGALLMLGNFGITLLGMR"
BASE COUNT          946 a         1605 c         1779 g          845 t
ORIGIN      
        1 tcgattatat gtactatctc gagcgcgcct gccagaccca ggtgaccgcg atggcgggcg
       61 gcgcgaaact gcgcatcccc gcgccggccg tcgccgcgaa aacggcggat cagttccggg
      121 cgctgccgta caagccgaag cgcccggaat ggaaggcgct gctgcggatg ctggacaaga
      181 ccgacccgac gtacaagaat tagtgtcgag tgtcgagtgt cgagggacga gggatgaggg
      241 atgagggatg agggatgagg gatgagggat gagggacgag ggatgaggga tgagggacga
      301 gggatgaggg atgagggatg agggacgagg gatgagggac gagggacgag cgaacaaaac
      361 gttacagagc cttacccctc gacactcgac actcgacact cgacactcga cacccgacac
      421 ccgacactcg tcacccgaca cccgagcaac gagtcacttt ctcagtcaac ggccggcggc
      481 gcggcgcaaa cctgctcgta gcggtagcgc tgcttgagca ggccgttgta ttcgtacacg
      541 tccagtgttt tttcgtacgc cgcgcgcgtg atctcgacgt gcggcgtcca gcagccgagc
      601 ttctggtagg tcgcgatgca atcggcgagg acggactcgc cgatgtcggg gaaaaacggc
      661 ttctgcgccc tggcgatcgc ggcggccggc gtcgcgatca tgtaggcgcg cgttctccgg
      721 taggcgcgga tgaacgtctt cgccatcccc gtattcagcc actcgcgcgt cgccgccagg
      781 ctggaaaagc cgcacggccc gatctgcggg ccgacctggg ccacgatatg gccgacaccg
      841 tcggcctgca gttgctgcgg gtatgggccc tgctgatgga cgtactgtcc cttgccgtcg
      901 cggaaggcgc ggtcgatctg ggcggcgccg ccggggcaga tcggcttgat tctgccgaag
      961 tcgatgcccg ccttgtggca ggcgtactgg aacatggcga gcggctggcc gccctcgaac
     1021 atgacgacct cggcgccttc cagtttcttc cacgtgaacg cgggatccgc gccgcggccg
     1081 gtgaggaaga atccgtccat ctcgttgacc tgggcgaaat gcaccgttgc cggcgtctcg
     1141 cccttgttga gggtggtgaa gccctggctc agcgccgact gcgcgacgtg cgccgtgcca
     1201 tccgtcagcg cggccagcgc cgagacgccc ggcggcgaga tcgaccactc gggatccagg
     1261 ccctcggcct tgaggaatcc gcccgacatc gccgagatga gcggcgagta gaacgcggag
     1321 aacagcgtga actggatttt gatcttttcc aaggcgattg cctcctcgcg cgcgtctgtg
     1381 ccgattgcgc gggtaacatc ctaagccatt tcgaaggcaa ggcggaagat gaggcgtacc
     1441 aggtgaagcg tcccggatcg ggaatattgc gtaagtccca atttgggact acactgcgcc
     1501 atgcgcgtca tcgccctgtc caccttgcgg gcgttctgga ccggcaaggc ggcttacgcc
     1561 gacgcgcgcg agccgacgct ggcgtggtac agacatgtgc ttcaggcgga ctggcgctct
     1621 cccgcggagg tcaagcgtga attcggcagt gcgagcattc tgcgtgacgg acgggtggtg
     1681 ttcaatattg ccggcaacaa gtaccgcgtc gtgacctgga tcaattatcc gtatcgcgtc
     1741 gtgtacatcc ggttcatagg tactcacgcg caatacgaca ggatagacgc gcagtccatc
     1801 tgaaaaggcg acgatatgga aatcaggccg atcaagacca aggcggatta ccgcgctgcg
     1861 ctgaaggaga tcgaagcgtt gatggcggcg gaacgcgaca cgccggaagg cgagcgcctg
     1921 gatgtgctgg tcacgctggt cgaggcgtac gagcgcaagc actaccggtt cgatcttccc
     1981 gacccggtgg aagcgatcaa atttcgcatg gagcagaagg gaatgacgcc caaggacctg
     2041 gtgccgatga tcgggcgcat caaccgagtg tacgaaatcc tgagccgcaa gcgcccgctg
     2101 acactcgcga tggtccagcg gctgcacagc gggctgggta tacccgccga atccctgatc
     2161 aagcagcccg acaggcgcaa ggcagcctga ttccctgacg aatggcaata aatgaggcca
     2221 tcacggcccg ctcactaccg ggccggacta ccgcaacccc ggcgctggca tcggaatacc
     2281 cgagcgaaac acgggggctg gcacgcgttt tggcccatgc cttcgtcgct cgccttgcta
     2341 atatcggcat attggccgcg gctcgcttcg cgctctgagc caaaatcgtg acagcgcggc
     2401 gcacgtgtca ttatgtcagg gacttttgag gaggatttat gaaggcaagg tttcccgttt
     2461 gcatgctcgc ggccgggctg gcggccgcgc tcgccgcgcc ggcccacgcc cagaattacc
     2521 cggtcaagcc gatccgcatc gtgacttcgc ccgcgggcgg cggcaacgat ttcccggcgc
     2581 ggctgatcgc gcgctcgctc cccggtccgc tcgggcagca gatcgtcgtc gacaaccggg
     2641 ccacgatcct catcgcggac atcgtcgcca agtcgccgcc ggacggctac acgctgctgg
     2701 tgaccggcag cgcgcactgg atcgggccgc tgctcgacaa ggtgacctac gatccgatcc
     2761 gggatttcgc gccgatcacg ctgatcgacc gggcgccgag tgtgctcgtc gtgcatccgt
     2821 cgatgccgct gaagtcgatc aagcagttga tcgcgctcgc cagggcgagg cccggcgaga
     2881 tgaatttctc ggtcggcggg ccgggcagct cgggctacct gggcgcgatc ctcttcaatc
     2941 acatggcggg cgtgaacatc gtgagcgtgc cgtacaaggg cacggcgccc gcgcttgccg
     3001 ccgtgatgag cggcgaagtg cacgcgatgt tcggctcggc cggcagcgtc gccccgcacg
     3061 cggcgtcggg acgcctgcgc ccgctcgcgg tgggcagcgc ccagcgctcc cggctcgcgc
     3121 cgggactgcc cacggtcgcg gaggcgggcc tgcccggctt cctgtcggag gcgctgcacg
     3181 ggctgttcgc gccggcgggc acgccggctg cggccatcgc gctgctcaac cgcgaggtcg
     3241 gcaattacct gcgctcgccc gaagcgcagg gcattttcct caaggcgggc atcgagcctt
     3301 cgccgggggc gcccgaggag ctgacggcga tcatgaaatc ggaaatgacg cgcatcggcg
     3361 cggtgctcaa ggccaccggc accgcgcggc gctgacgcgc tgcccggaag tgcttaagga
     3421 aaaagggaaa aggaaaaagg gaaaaatgtc gagaaacgag tgatggatga tgagggcctg
     3481 gctttttgaa aagctttcct ttgtgtcctt cgtgtccttc gtgtcgaggc ttttttcaat
     3541 ttcgtaggtg cagggagaac cgatggggca gctgcaaggc aagatcgcgc tcgtggcggg
     3601 cgcgggccgc aacaacggac gagcgatcgc gctcgccttc gcgcgcgaag gcgccgatct
     3661 cgtgctggtc gcgcgggaga agcagtcggc gctggatgaa gtcgcgcacg agtgcgagaa
     3721 gctcggcgcc cgggtgctgc ccctgctctg cgacgtgagc gagcacgagc aggtgagccg
     3781 catggcgcag caggccctcg gccatttcgg ccacatcgac gtgctgatgg gtgttgccgg
     3841 caggcgggcc caccaggatt tctgggagat cggcctcgag gagtggcaca agaccttcgc
     3901 ggtcaacctg cattcgctct tctatctcgc cagggcgatc gtgccctcga tgatggagcg
     3961 caggggcggg agcatcgtcg cgctcggcgg cctcgcctcc ctgaccgcgc agccgcagcg
     4021 cgcgcacgtg gtcgcgtcca agaccggcct ctacggcctc gtccgcgcgc tcgcgctcga
     4081 gctggggccc tacaacatcc gggccaacct catcgcgctc ggcatgatcg agaacgtgcg
     4141 cgcgaacccc gagtggtatc cggggaccca gggcggcgcc tacaaggatg acgagctcgc
     4201 cggcatcgcg ttgaaacgcg ccggcaagcc gcaggaagtg gcgaacgtcg ccgtgttcct
     4261 cgcgtccgac cagtcgtcgt tcgtcaccgg cgatcgcatc gtgtgcgccg gcggcaaata
     4321 cctatgactc cgggttgccc cgggcgctag cgcatcccga gcagcgtgat cccgaagttg
     4381 cccagcatca acagggcgcc gaccacgatg atcacgccgg cgcgccgcgg ataggcggcc
     4441 acccatttgt cgagcgacag gttctgggtg tccgcgccct tcaagagccg gcgttcggag
     4501 aaccagcggc tgccgccggc ttccagcgcg gccagggtgg cgggggaaaa ggcgagcagg
     4561 acgccgatcg cgatcgcaac caggttgccc gcgatcagga tccagcgggc gctgtcgacg
     4621 atccacgcgg ccaacgccgc cggcagagcc gccaggttga acaattgtat ggctgcgccg
     4681 gcgctgaacc gggtggcgag gtagtaggtc gcgtagacgc cgccggcgat gaagagcgcc
     4741 ccgagccagt agcggcgttt ttgcacggcc tgggtggtgt cgcggggaat ctcgagcggc
     4801 cgcgaggcgc tgcgcaggga cacccagcga ttcatggtgc cgaaaaaccg caacgtccgc
     4861 tggctgttta cgatcaggct cacgccgacg gcaacgcaca cgattccccc gatgaggaag
     4921 aacacggtca tgacctggcc gatccactgc cagaacgcgc tgcctttcaa tatcgcttcc
     4981 atgcggctct cccgggtggg gatgggttga atggtgcggt caattgtacg ggaaccccgg
     5041 aggttgcgcc caaacgagga gggagcagga gctatttaag gcacaaggcg gaagatgagg
     5101 cgtcgcaggt gaggcgtcgc agatgaagcg tcgcagatga agcgtcgcag atgaggcgtc
     5161 gcaggtgaag cgtcg
//
LOCUS       DBNGSG010000278         3648 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2635074, whole genome shotgun sequence.
ACCESSION   DBNGSG010000278 DBNGSG010000000
VERSION     DBNGSG010000278.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2635074"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="AC158Q_07220"
     CDS             <1..662
                     /locus_tag="AC158Q_07220"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HIR3339604.1"
                     /translation="IEIRDVEDLRPFTQLIDRLDEFDYAIFISPNSVERSMKLINARR
                     QLPPGLKIATIGGGGMRALEAHGVAGVIAPQDRHDSEGLLDLPAFAVVSFKRVVIFRG
                     VGGREHLSETLRERGAAVEYAECYYRYRPDLDPAPLLNAWARDELHAVTVTSSEGLHN
                     LFESVGEAGRGQLVRTPLFVPHPRIAEAAHGLEVQTVVVTGPGNDGLLTGLSAFFSSR
                     R"
     gene            764..1846
                     /locus_tag="AC158Q_07225"
     CDS             764..1846
                     /locus_tag="AC158Q_07225"
                     /inference="COORDINATES: protein motif:HMM:NF016275.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HIR3339605.1"
                     /translation="MNDSNQPDSSTTAPAPAAAGSTEAQGTGGLSAWRVPAALLFAAL
                     ALGLSAWQWHDSRGEIAALRQELAKKLSDTDAESKESRQIAEQVRETVADAQAKLGML
                     ESRLAESQSQQIALESLYQELSRNRDEWAYAEIEQSLFIASQQLTLAGNVKGALIALQ
                     AADTRLQRMNRPQLATLRKAINRDIERLKSAPQVDAVAISVRLDNLIAQADKLPLAMD
                     ARPQPAEPAAAPQQEADPWWSRFWRDTWTELKQLVRVQQMDLPDAALLAPAQSYFLRE
                     NLKLRLIGARLALLARDAASYKADLKAARDWLTRYYDTRHNSVAYALGLLRNLHDAEV
                     SIEAPDITASLEALRTLRAARERTAR"
     gene            1843..3051
                     /locus_tag="AC158Q_07230"
     CDS             1843..3051
                     /locus_tag="AC158Q_07230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HIR3339606.1"
                     /translation="MRSLLWLVGIVALAVALTLAARYNTGYLLLVRPPYRVELSLNVL
                     IVLLVAVFAAGYALVGLVSATLRLPRQAREFQLARRKEKARVTLTEALNAYFAGRYGR
                     AEKAAAAALDLGEQPGLAAVLAARAAHELRAYERRDAFLARAAAAASGDAVVHIVTAA
                     DLLLDERRYQDALNLLGQLPRRHTAALRLELKAQQQARNWEQVILITEELRKRNVYDL
                     TQAGEVRRHALAENLKRKALDARALEEAWDKAPPTDRRDRRVAIAAAQCFIGLGDFDQ
                     ARRIIEEALEAAWDSDLVGLYAGCEGGDAVKRIERAESWLEQHPSDAVLLLTLGRLCA
                     DQGLWGKAQSYLEASLAIEPTYSAHLALARLHERLGNAEATDRHYRESLGLALAQLRS
                     VTGGRRRTPF"
     gene            complement(3468..>3648)
                     /locus_tag="AC158Q_07235"
     CDS             complement(3468..>3648)
                     /locus_tag="AC158Q_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HIR3339607.1"
                     /translation="RVLSLAVIGGTLLLLACLGAVAARLGGAPVLAGALRVTLWGAVA
                     MAGTALVGMLFGAAP"
BASE COUNT          653 a         1174 c         1231 g          590 t
ORIGIN      
        1 tgatcgagat ccgcgatgtc gaggacctgc gtccgttcac gcagttgatc gaccggctcg
       61 atgagttcga ctacgcgatc ttcatcagcc ccaattcggt cgagcgctcg atgaaactga
      121 tcaacgcgcg ccgccagctt ccgccggggc tgaagatcgc cacgatcggt ggcgggggca
      181 tgagagcgct cgaagcccac ggcgttgccg gggtcatcgc gccgcaggat cgccacgaca
      241 gcgagggact gctggatctg cctgcgttcg cggttgtgag tttcaaacgc gtggtgatat
      301 tccggggcgt gggcgggcgc gagcacttga gcgagacatt gcgcgagcgc ggcgcggcgg
      361 tcgaatacgc cgagtgttac taccgctaca ggcccgatct cgatcccgcg cctttgctca
      421 atgcctgggc gcgcgacgag ctgcatgcgg tcacggtgac gagcagtgaa ggactgcaca
      481 acctgttcga atcggttggc gaggccggtc gcggtcagct cgtgcgcact ccgctgttcg
      541 tgccgcaccc gcggatcgcg gaggccgcgc acggactcga ggttcaaacg gtggtcgtga
      601 ccggtcccgg caacgatggg ctgctgaccg ggctgagcgc ttttttcagt tcccggcgct
      661 gaaagtccga tctggcgtgg gcggtgtccc ggagttcgcg ctttgcattg cgagccggca
      721 gcgacgcctc catactatcg cttgatcgcg cattgttccc ggcatgaacg attccaacca
      781 gccagactcg tcgacaaccg ctcccgcgcc ggccgcggca ggcagcaccg aggcgcaagg
      841 taccgggggc ttgtccgcat ggcgtgtgcc cgcggcattg ctgttcgccg cgttggcgct
      901 gggcctgtcc gcgtggcagt ggcatgacag ccgcggcgaa atcgccgcct tgcgccagga
      961 actcgcgaaa aagctctccg ataccgatgc cgagagcaag gaaagccgcc agatcgccga
     1021 gcaggtgcgg gaaaccgtgg ccgacgccca ggcgaagctc gggatgctcg agagccggct
     1081 cgccgagtcg cagagccagc agatcgcgct cgagtcgttg taccaggagc tttcgcgcaa
     1141 ccgcgacgag tgggcctacg ccgagatcga gcaatcgctg ttcatcgcca gccagcagct
     1201 gactcttgcc ggcaacgtca agggggcgct gatcgccctg caggcggccg acacgcggct
     1261 gcagcgcatg aaccggccgc aactggcgac gttgcgcaag gcgatcaacc gggacatcga
     1321 acggctgaag tcggccccgc aagtggatgc ggtggcgatc agcgtgcggc tcgataacct
     1381 cattgcgcag gcggacaaac tgccgctcgc catggatgcg cgaccgcagc cggccgagcc
     1441 ggccgccgcg ccgcagcagg aggcggaccc ttggtggtcg cggttctggc gtgacacctg
     1501 gacggagctg aaacaactgg tgcgggtgca gcagatggac ctgccggacg cggcgctgct
     1561 ggcaccggcg cagtcctatt tcctgcgcga gaacctgaag ctgcggctga tcggcgcgcg
     1621 gctggcattg cttgcacgcg atgcggcgag ctacaaggcg gacctgaagg cggcgcgcga
     1681 ctggcttacc cgctactatg acacgcgcca caacagcgtc gcctacgcgc tgggcctgtt
     1741 gcgcaacctg cacgacgccg aggtcagcat cgaggcgccg gacatcaccg catcccttga
     1801 agccctgcgc accttgcggg ccgcccgcga gcgcactgcg cgatgaggtc gctcctgtgg
     1861 ctggtcggca tcgtggcgct ggcggtggcg ctcacgctgg cggcgcgcta caacaccggc
     1921 tacctgctgc tggtgcggcc gccgtaccgc gtcgaacttt ctctcaatgt tctgatcgtg
     1981 ctgctggtgg cggttttcgc ggcaggttat gccctggtgg ggctggtttc cgcgacactg
     2041 cgcctgccgc gccaggcgcg tgaattccaa ctggcgcgcc gcaaggaaaa ggcgcgcgtg
     2101 acgctcaccg aggcgctcaa cgcgtatttt gccggccgct acggccgggc ggagaaagcc
     2161 gccgccgccg cgctcgacct gggcgagcag cccgggcttg ccgccgtgct ggcggcacgc
     2221 gccgcgcacg agttgcgcgc ctacgagcgg cgcgatgcct ttctcgcgcg cgctgctgcc
     2281 gccgcatccg gcgacgcggt agtgcacatc gttaccgccg ccgacctgct tctcgacgag
     2341 cgccgctacc aggacgcgct gaacctcctc ggccagctgc cgcgccggca taccgcggcg
     2401 ctccgcctcg agctgaaggc gcagcagcag gcgcgcaact gggaacaggt catcctgatt
     2461 accgaggaac tgcgcaagcg caatgtatac gatttgacgc aggcgggtga agtgcgccgc
     2521 cacgcgctcg cggagaatct caagcgcaag gcgctcgacg cgcgcgcgct ggaggaggcc
     2581 tgggacaagg ccccgccaac ggaccgccgg gaccggcgtg ttgcgattgc tgccgcgcaa
     2641 tgcttcatcg gcctcggcga cttcgaccag gcgcgccgca tcatcgagga agcgctggag
     2701 gcggcctggg acagcgatct cgtcggcctt tacgccggat gcgagggcgg cgacgccgtc
     2761 aagcgcatcg agcgcgccga gtcctggctg gagcagcacc cgagcgatgc cgtgctattg
     2821 ctgacgctcg gccggctatg cgccgaccag gggctgtggg gcaaggccca gagttatctc
     2881 gaggcgagcc tcgcgatcga gccgacttac tccgctcatc tcgcgctcgc gcggctgcac
     2941 gagcggctgg gcaacgcgga agcgaccgac cggcattatc gcgagagcct cgggctcgcg
     3001 ctggcccagc tgcgcagtgt caccggtggc cgccgtcgaa caccgttttg attagccgca
     3061 gagacgcaaa ggcgcaaaga aaacgatcat cgaaccgcca agcacgccaa gagaaacaga
     3121 attagccaca gagaacacag agagcacaga gaacagcaga atcaagacga gaagaaaatc
     3181 atggccggct gcaaatagcc ggccaattca tttgatcgct ggtgatacga gattaacttg
     3241 tattagatga cagtcatcta gttgggaaaa gtatataccg gcagcgagaa atcactatta
     3301 gtagcgataa atccccagta atagtgagtt gcagctgcca ggccttgatc ttttatcgtg
     3361 tatttcctgg cgtgcttggc ggttcgattg cttttcttgg cgccttggcg ccttggcgtc
     3421 ttggcggcta atttattact agcgggttag acgttatcgc ttccagacta aggcgccgcg
     3481 ccgaacagca ttccaaccag ggcggtgccg gccatcgcga cagcgcccca gagagtgacc
     3541 cgcagcgcgc cggccaggac cggggcgccg cccagccggg cggcaacggc gcccaggcaa
     3601 gcgagcagca gcagcgtgcc gccgatcacg gcgagggaaa gtaccctg
//
LOCUS       DBNGSG010000279         1934 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_427920, whole genome shotgun sequence.
ACCESSION   DBNGSG010000279 DBNGSG010000000
VERSION     DBNGSG010000279.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_427920"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..543
                     /locus_tag="AC158Q_07240"
     CDS             199..543
                     /locus_tag="AC158Q_07240"
                     /inference="COORDINATES: protein motif:HMM:NF026905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB domain-containing protein"
                     /protein_id="HIR3339608.1"
                     /translation="MRRGKTRTLYASLIRMISRQEARALVEAEINRPDPCGPRGAVEI
                     VEESTLERSWGWVFFYQSSEYLRTGNVSAMLAGNSPYIVNRHTGEVIVTGTAWPTRKY
                     IDDYESRLRSGV"
     gene            628..999
                     /locus_tag="AC158Q_07245"
     CDS             628..999
                     /locus_tag="AC158Q_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajD family HNH nuclease"
                     /protein_id="HIR3339609.1"
                     /translation="MKKSSPDTGRLDRVIAEDRRERAAREKGYRAQALKLYPWVCGRC
                     AREFTHANLQLLEVHHRDGNHDNNPPDGSNWELLCTYCHEAEHRKLLDNTSSGDGDKS
                     AATHKPFEALAELLKRKDRES"
     gene            1149..>1934
                     /locus_tag="AC158Q_07250"
     CDS             1149..>1934
                     /locus_tag="AC158Q_07250"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3339610.1"
                     /translation="MRMPDYQVTGAGKVTVFLLHGIYGSKEYWRPQTERLVARGYRVV
                     AWDAPGYGLSPLPDPFGFEVVADAGAQLIKAQGTERNVVHGHSMGGQITPRIRLKAPD
                     LVHGIVISSTIGYFGNRTKEEQEEFVRKRTAPPPPGTDPVTVNRMVVDSMLGPTSKGP
                     EVDLVREWASRTPPDTVKAAVKAVQTFPEADAVAGFRAIDVPTLLVAGEVDQVGHPAG
                     MRRVADMIKGCEFATVSGSGHYPWAENPAEFNAAFFGYLSMHFR"
BASE COUNT          376 a          616 c          599 g          343 t
ORIGIN      
        1 gatgcacagg ctcgccgcat tcgtgatctc gaggccctcc tgctcagcgt cctccaaccg
       61 atccccaaat ggaacaaggt caagaccaat ttcgtcggag cgcggcgact tgccatccca
      121 gcttgaccgt gcgcactaca cgagtgcccg gcctaacagc gcgctgttga ccgacgcctt
      181 cagctcgcta cgctgcgcat gcggcgcggc aaaacgcgaa cgttatacgc ctcgctcatc
      241 cgcatgattt cacggcagga agcgcgcgca ctggtcgaag cggagatcaa ccggccggat
      301 ccctgcggcc cacgcggtgc tgtcgagatc gtcgaggagt ccacgctcga gcgaagctgg
      361 ggttgggtct tcttctacca gtcgtcggag tacttgcgaa cgggaaacgt gtccgcaatg
      421 ctggccggca actcgcccta catcgtcaac cgacacaccg gtgaagtcat cgttacgggg
      481 accgcgtggc ccacacggaa gtacatcgat gattacgagt cacggttgcg ttccggcgta
      541 taacaagcag ttggagcgga ccgtcatgcg gcgtcacttt ctaggatgtc gaatcttgta
      601 aatcttgtta atcctgtctc tgtagctttg aaaaagtctt cgcctgacac cggccggctg
      661 gaccgtgtaa tcgcggaaga ccggcgcgag cgggccgcgc gcgaaaaggg ctatcgggcg
      721 caggcgctca agctatatcc atgggtctgc ggccgctgtg cgcgagaatt cacgcacgcg
      781 aacctgcagc tgctcgaggt ccatcaccgc gacggcaatc acgacaacaa cccgcccgat
      841 ggcagcaact gggagctgct ctgcacctac tgccacgaag ccgagcaccg caagctgctc
      901 gacaacacga gcagcggtga cggagacaaa agcgcggcga cccacaagcc gttcgaggct
      961 ctcgccgaac tgctcaagcg gaaggatcgc gaatcgtaaa acgtgaatcg gaaatcgtaa
     1021 cccccttcca accctccccc ttggcaaggg ggagggccag ggtgggggtt tgaatcctgt
     1081 taatcttgtt aatcctgtct atttgttttt cttggcggcc ttggcggtta attgaagggg
     1141 tttagcaaat gcgaatgccg gattaccagg tcaccggcgc aggcaaggtc acggtctttc
     1201 tcctgcacgg catttacggc tccaaggaat actggcgccc ccagaccgaa cgcctggtcg
     1261 cgcgcggcta ccgcgtggtg gcgtgggacg cgccgggata cgggctctcg cccctgcccg
     1321 acccgttcgg gttcgaagtc gtcgccgatg ccggcgcgca actcatcaag gcgcagggca
     1381 ccgagcgcaa cgtcgttcac ggccacagca tgggcgggca gatcacgccg cgcatccgcc
     1441 tgaaggcgcc ggatctcgtt cacggcatcg tgatttcctc gacgatcggc tatttcggca
     1501 accgcaccaa ggaagagcag gaggaattcg tccgcaagcg gaccgcgccc ccgccgcccg
     1561 gaacggaccc ggtcacggtc aaccggatgg tggtggactc gatgctcggg ccgacctcca
     1621 agggacctga ggtcgacctc gtgcgcgagt gggcttcgcg caccccgccc gacacggtga
     1681 aggccgcggt gaaggcggtg cagacgtttc ccgaggcgga cgcggtggcg ggattccgcg
     1741 cgatcgacgt accgaccctt ctggtggcgg gtgaagtgga ccaggtcggc caccccgccg
     1801 gcatgaggcg cgtggccgac atgatcaagg gctgcgaatt cgcgaccgtc tccggctccg
     1861 gccattatcc ctgggcggag aatcccgccg agttcaatgc cgccttcttc gggtacctta
     1921 gcatgcactt ccgc
//
LOCUS       DBNGSG010000280         4260 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_938098, whole genome shotgun sequence.
ACCESSION   DBNGSG010000280 DBNGSG010000000
VERSION     DBNGSG010000280.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4260)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_938098"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="AC158Q_07255"
     CDS             <1..363
                     /locus_tag="AC158Q_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HIR3339611.1"
                     /translation="PAKEVLGAAKEREAIPYDVPDVHFSHDGELCSFDAFLKTYRLTG
                     DPALARLAVIVRGADTARLDLAPQAAGLLAVSLGLSRNFTDDHEMLKRGMVMYDALYA
                     WCREGQDETHTWNPAAYR"
     gene            complement(419..1372)
                     /locus_tag="AC158Q_07260"
     CDS             complement(419..1372)
                     /locus_tag="AC158Q_07260"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3339612.1"
                     /translation="MIRTALISLLLLACPLPAAAQYAERPLTILSGYPAGGMVDIVAR
                     LLAEGMKGKFPKGVLVANRTGAGGALAAAEVATGKPDGTTIVLTPLSTLVIQPQILDV
                     RFKTPDDYEPIINVVSYYPLLVVQAEARWKNVQEFIAEAKANPGKMRVGSPGEGTSSH
                     LNLEELMHVSGIKVTHVPYRGWGESSPALLGGHIEAIVAQPGEAKPMVEGKRMRALVV
                     FQNTPHPAFPGTPTAKELGWNVANGVWFLLMAPKGTPAPVIKQIHDAAKAAIEEPAFK
                     AAMDLRGVDVDYRPGSQLRTELWREYKAHTAILKRIGMIKQ"
     gene            complement(1466..1894)
                     /gene="fur"
                     /locus_tag="AC158Q_07265"
     CDS             complement(1466..1894)
                     /gene="fur"
                     /locus_tag="AC158Q_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HIR3339613.1"
                     /translation="MSSPNDLKTIGLKATLPRLRILELFEKSPVRHLSAEDVYKTLSK
                     DGTDTGLATVYRVLTQFEQAGLLIRHHFETGKAVFELNQGGHHDHLVCVQCGHVEEFF
                     DPEIEKRQEKIAKDRGFKIHDHSLHLYAECTKANCPHKNR"
     gene            1944..2372
                     /locus_tag="AC158Q_07270"
     CDS             1944..2372
                     /locus_tag="AC158Q_07270"
                     /inference="COORDINATES: protein motif:HMM:NF016258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990063 - Bam protein complex [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HIR3339614.1"
                     /translation="MRLRHLTALVLLLAGCSKQMLPTLPGLTPYKMDVQQGNVVTQEM
                     IAKVQPGMSRSQVRFALGTPLIVDPFHSDRWDYVYFYQKGGVTTEQRRIAVIFKDDRL
                     VRIDGDVVPAGAGGATPPAKTDRPAVPAPAATPQPQAESK"
     gene            2394..3209
                     /gene="dapB"
                     /locus_tag="AC158Q_07275"
     CDS             2394..3209
                     /gene="dapB"
                     /locus_tag="AC158Q_07275"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HIR3339615.1"
                     /translation="MTKLKIAVAGSTGRMGRALLEAVLRSSDFSLAAALEAKGNAAAG
                     KDAGELVGSPCGVKISDDAAKAIAGCDVLIDFTRPEATVAYLAACRKQRVKMVIGTTG
                     FSEAQKKEIANAAREIAIVMSPNFSVGVNVAFRLIEVAAGALGRGYDVEIVEAHHRHK
                     VDAPSGTALRMGEVVAQALGRDLKKHAVYGREGVTGERRDETIGFASVRGGDTVGDHS
                     VMFIGTGERLEIAHHASSRANFASGALRAARFLAPQKQGLFDMADVLGFARKG"
     gene            3385..>4260
                     /gene="carA"
                     /locus_tag="AC158Q_07280"
     CDS             3385..>4260
                     /gene="carA"
                     /locus_tag="AC158Q_07280"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - L-glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HIR3339616.1"
                     /translation="MSRIPAILVLKDGTVFRGTSIGAEGLAAGEVVFNTAMTGYQEIL
                     TDPSYCRQLVTLTYPHIGNTGVNSVDVESDRIYAAGLVIRDLPLLASNWRKQWTLSDY
                     LKQHGVPAIADIDTRRLTRLLRDKGAQDGCLMTGKVNEKEALKAARGFPGLVGMDLAR
                     VVSCGKPYGWDEGTWALGQGYSRLTEPRFHVVAYDFGVKRNILRKLASRYCRVTVLPA
                     QTPADEALALKPDGVFLSNGPGDPEPCDYAIKAIRKFLDIGIPTFGICLGHQLMGLAS
                     GAKTLKMKFGHHGANH"
BASE COUNT          739 a         1339 c         1395 g          787 t
ORIGIN      
        1 ccggcgaagg aggtgttggg cgcggcgaag gagagggagg cgattccgta cgacgtgccg
       61 gacgtgcatt tctcgcacga cggcgagttg tgcagcttcg atgccttcct caagacctac
      121 cggctcaccg gcgacccggc gctggcccga ctcgcggtga tagtgcgggg agcggacacg
      181 gcccgcctcg atctcgcacc gcaggcggcc gggctgctcg cggtttcact gggcctgtcc
      241 cgcaatttta ctgacgacca cgagatgctg aagcgcggaa tggtgatgta cgacgcgctc
      301 tacgcctggt gcagggaggg gcaggacgag acgcatacct ggaaccctgc ggcttaccgc
      361 taaatcttta ccgccaaggc gccttggcgg ttaatttctg gttctagccg tcactgcgtc
      421 actgtttaat cattccgatc cgcttcagaa tcgcggtgtg cgccttgtat tcccgccaca
      481 actcggtgcg gagctggctg ccgggtcggt agtcgacgtc cacgccgcgc aggtccatcg
      541 cagccttgaa tgccggctcc tcgatcgccg ccttggccgc gtcgtgtatc tgcttgatca
      601 caggcgcggg cgtgcccttc ggcgccatca gcaggaacca gacgccgttc gcgacgttcc
      661 agcccagttc cttcgccgtg ggcgtgcccg ggaacgcggg atggggcgtg ttctggaaca
      721 ccaccagcgc gcgcatcctc ttgccttcca ccatgggctt cgcctctccg ggctgcgcga
      781 caatcgcctc aatgtgcccg ccgagcaagg ccggtgacga ctcgccccag ccacggtagg
      841 gaacgtgcgt caccttgatt ccgctcacgt gcatcagctc ttcgagattc aggtggctgg
      901 acgtgccctc gccgggcgag ccaacgcgca tcttgcccgg gttcgctttt gcctctgcga
      961 tgaattcctg gacgttcttc cagcgcgctt cggcttgcac caccagcagc gggtaatacg
     1021 acaccacgtt gatgatgggc tcgtagtcgt cgggcgtctt gaagcgaaca tccaggatct
     1081 gcggctggat gaccagcgtg gacagcggcg tgagcacgat ggtggtgccg tccggcttgc
     1141 ccgtggcgac ttccgcggca gctaacgcgc ctcccgcgcc ggtccggttg gccaccagga
     1201 cccccttcgg aaacttgccc ttcatgcctt cggccagcag gcgcgcgacg atgtccacca
     1261 tcccgccggc ggggtagcct gacaatatcg tcagcggccg ctccgcgtat tgcgctgccg
     1321 cgggcagggg gcaagccagt aacagcagac tgatcagtgc agtgcgaatc atgtgctgtc
     1381 ctccttcaca ttcacccgcg tgttgagcgg cagcaaattc atccgttcga acgcgcgaag
     1441 tcaaaccggc gcgaagtgtc cggacttaac ggttcttgtg cgggcagttc gctttcgtgc
     1501 actcggcgta gagatgcagc gagtggtcgt ggatcttgaa gccgcggtcc ttggcgattt
     1561 tttcctgccg cttctcgatc tcggggtcga agaattcctc gacgtgcccg cattgcacgc
     1621 acacgaggtg gtcgtggtgc ccgccctgat tcagctcgaa caccgccttg ccggtttcga
     1681 aatggtgccg gatcagcagc cccgcctgct cgaactgggt gagtacccgg tacaccgtcg
     1741 caagcccggt atcggtgccg tccttcgaga gggttttgta gacgtcctcg gccgagaggt
     1801 ggcgcaccgg actcttctcg aacagctcca gtatgcgcag gcgcggcagc gtcgctttca
     1861 ggcctatcgt tttgagatcg ttgggggaac tcaccgctcg tcactgtgcg aatagttggg
     1921 cgtcggttat catattcgga ttcatgcgac tacgccatct caccgccctc gtgctgctac
     1981 tcgctggctg ctcgaaacag atgctgccca cgctgccggg gctgacgccg tacaagatgg
     2041 acgttcagca gggcaacgtc gttacccagg aaatgattgc caaggtccag cccggcatga
     2101 gccgcagcca ggtgcgcttt gcgctcggca cgccgctcat cgtggacccg ttccacagcg
     2161 accgctggga ctatgtttat ttctaccaga agggcggcgt gacgaccgag cagcgcagga
     2221 tagcggtcat attcaaggat gacaggctgg tgcggatcga cggtgatgtc gtgccggccg
     2281 gcgcgggcgg tgcgacgccc cccgcgaaaa cggacaggcc ggctgtcccg gcaccggcgg
     2341 ccactccgca gccgcaggcg gaaagcaaat agcgccgacg ggatggttac gtgatgacga
     2401 agctcaaaat tgcagttgcc ggcagcacgg gccggatggg gcgcgcgctg ctcgaggcgg
     2461 tgctgcgatc atcggatttc agtctcgcag cggcgcttga ggcaaagggc aatgccgccg
     2521 ccggcaagga cgccggcgag ctggtcggct cgccttgcgg cgtgaaaatc agtgacgacg
     2581 cggcgaaagc catcgcgggc tgcgacgtgc tgatcgactt cacccggccg gaagccacgg
     2641 tcgcctacct ggccgcctgc cgcaagcagc gcgtgaagat ggtgatcggc acgaccggct
     2701 tcagcgaggc gcagaagaag gagatcgcga acgcggcacg cgaaatcgcg atcgtgatgt
     2761 cgcccaactt cagcgtcggc gtcaacgtcg cgtttcgtct gatcgaggtg gcggcgggcg
     2821 cgctcggccg gggttacgac gtcgaaatcg tcgaggcgca ccaccggcac aaagtggacg
     2881 cgccttccgg caccgccctg cgcatgggag aagtggtggc gcaggcgctc gggcgcgacc
     2941 tgaaaaaaca cgcggtctac ggccgcgagg gcgttaccgg cgaacgcagg gatgagacca
     3001 tcggttttgc ctccgtgcgc ggcggcgaca cggtgggcga ccacagcgtg atgttcatcg
     3061 gcaccggcga gcggctcgag attgcccacc acgcctccag ccgcgccaac ttcgccagcg
     3121 gcgcgttgcg cgccgcgcgc ttcctggcgc cgcagaagca aggtctcttc gacatggccg
     3181 atgtacttgg cttcgccagg aagggatgag gtatgagggc ggagggatga gggaaaagcc
     3241 cagttttttg tagtgttcac ccatttagca gcctccggcg cgttgctgcc ggaggctgtt
     3301 tcgcatataa ttcactcggt tggcgggagg ggctgaaaag cctctcccgc tttgtgtata
     3361 tacacctgaa atcaaggggt tagtgtgtca cggattcctg ccatcctggt gctcaaagat
     3421 ggcacagttt tccgcggcac ttcgatcggt gccgagggcc tcgccgcagg ggaggtggtg
     3481 ttcaacaccg ccatgaccgg ctaccaggaa atcctcaccg acccgtccta ctgccggcaa
     3541 ctcgtcaccc tcacttaccc gcacatcggc aacactggag tcaattcggt ggacgtcgag
     3601 tcggaccgga tctacgccgc gggcctggtc atccgcgatc tgccgctgct ggcctcgaac
     3661 tggcgcaagc agtggacgct ttccgattac ctgaagcagc acggcgtgcc cgcgatcgcc
     3721 gacatcgaca cgcggcggtt gacgcgcctg ctgcgcgaca agggggcgca ggacggctgc
     3781 ctgatgacgg ggaaggtgaa cgagaaagaa gcgctcaagg ccgcgcgcgg ttttcccggg
     3841 ctggtcggca tggatctcgc ccgggtggtg agctgcggca agccctacgg gtgggacgag
     3901 ggcacgtggg cgctggggca gggctacagc cgcctcaccg agccgcgttt tcacgtcgtc
     3961 gcctacgatt tcggcgtaaa gcgaaacatt ttgaggaagc ttgcatcgcg ctactgccgc
     4021 gtgacggtgc tcccggcgca gacgcccgcg gacgaggcgc tcgcgctgaa gccggatggc
     4081 gtgttcctct ccaacgggcc cggcgacccc gagccgtgcg attacgccat caaggcgatc
     4141 cgcaaattcc tcgatatcgg catcccgacg ttcggcattt gcctcgggca ccaattgatg
     4201 gggctcgcaa gcggtgcgaa gaccctgaag atgaagttcg ggcaccacgg cgcgaaccat
//
LOCUS       DBNGSG010000281         5651 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1616880, whole genome shotgun sequence.
ACCESSION   DBNGSG010000281 DBNGSG010000000
VERSION     DBNGSG010000281.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5651)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5651)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5651
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1616880"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="AC158Q_07285"
     CDS             complement(<1..777)
                     /locus_tag="AC158Q_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HIR3339617.1"
                     /translation="MAKRIWDDFLTERDRQVMAAAGYGALQGYGKRPALMVIDVNYFF
                     CGDRPEPILESIKRWRNSCGEDAWPAIKVIGRLLEAARGKGVPVIYTTGTARADLWNR
                     GSWLWINDRAGEDSAVRRPRNVDGNAIIPDIAPAPRDIVVRKEKPSGFFGTPLQSYLV
                     LLGCDSLIVTGTTTSGCVRATVTEAFSNNYRVTVVEDACFDRSQASHAVNLCDMNAKM
                     ADVRPSAEVLEFLKQYPAGQFKLPSGEGMDGATPAPAVASG"
     gene            complement(808..1812)
                     /locus_tag="AC158Q_07290"
     CDS             complement(808..1812)
                     /locus_tag="AC158Q_07290"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3339618.1"
                     /translation="MTDGYSSREHRRALARRVACAALVLCCAAVGAASAQTYPARPIR
                     ILLPFGPGGVTDITARLLAQRLTDSLKQQVIIDNRPGAGGIVATELARKAEPDGHTIM
                     WLTTGHALAVAMFKSLPYDPVKDFRPVSTVGFFALALVVTGESPVKSVPALIAAVKVN
                     PARFNFAITNIGSSHHLSSELFKSMTGLPAQIVPFKTTPALVSALRSGEAQAAFEFIS
                     PVLPHLKSGALRALAVTSARRFAGLPEIPTVAEAGLPGFEVSAWNGTAVPAGTPRRVI
                     DRLNREVNAALAIPEVKARLQELGIEARPSTPEGLRDLLAAEIAKWNGVVDKAKIQRQ
                     "
     gene            complement(2022..>5651)
                     /gene="metH"
                     /locus_tag="AC158Q_07295"
     CDS             complement(2022..>5651)
                     /gene="metH"
                     /locus_tag="AC158Q_07295"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HIR3339619.1"
                     /translation="YRLTEADYRGSRLRDHPHDLKGNNDLLTLTRPQVIREIHEQYLA
                     AGADIIETNTFNSTSIAQADYHLQHLVRELNVEAAKLARAAAEAYTAKNPGKPRFVAG
                     ALGPTNRTASISPDVNDPGFRNVTFDELKTAYSEAVRGLVEGGVDILLLETIFDTLNA
                     KAALFAIDEFFEEQGIRLPVMISGTITDASGRTLSGQTPEAFWNSVRHAHPLAVGLNC
                     ALGAKLMRPYIEELSGVADIPVFAYPNAGLPNPLAPTGYDETPEQTAAFLLDFAKSGF
                     INIAGGCCGTTPAHIQAIAEALKDMPPRAVPVIEHKTRLAGLEPLNIGDDSLFVNIGE
                     RTNVTGSKAFARLILAGDYPAALSVARQQVESGAQMIDVNMDEAMLDSKQAMTTFLGL
                     IASEPDISRVPVMIDSSKWEVIEAGLKCVQGKCVVNSISMKEGREEFVKHARLARRYG
                     AAVVVMAFDEKGQADTLERRIGISRTAYDILVNEVGFPPEDVIIDPNIFAIATGIEEH
                     ANYGKDFIEATRWIRQNLPHARVSGGLSNISFSFRGNDRVREAIHTAFLYHAIKAGLT
                     MAIVNAGQIGVYDEIPKDLLEHAEDIIFNRRPDAAERMVTFAETVKGKGREQVEDLAW
                     RKGPVEERITHALVRGITDFIVEDTEEARQKLEKAGGRPIQVIEGPLMDGMNVVGDLF
                     GSGKMFLPQVVKSARVMKQAVAHLIPYIEAEKARVGDARPKGKVVIATVKGDVHDIGK
                     NIVSVVLQCNNYEVINLGVMVPWKKIFEVARDECCDIIGLSGLITPSLEEMAHNAREM
                     EREGLTIPLLIGGATTSRTHTAVKIAPNYSGPVIWVPDASRSVSVCSNLLSDDLRAGY
                     LKDVKSEYERVRTQHQGKKGPELITLEAARANAFKTDWAAYVPPRPEMPGVKLLKNYD
                     LAEIAEHIDWGPFFQTWELSGPFPAILEDPVVGEAARNVYAEGRAMLKKIIEGRWLTA
                     NAVFGLFRAVSVNSDDIEIYGDEPRGRPLMTWHNLRQQNVKPTGNPNLCLADFIAPKA
                     SGVRDTIGAFAVTAGIGIEKQIAKFEKQHDDYSGIMLQALADRLAEAFTELLHKRVRR
                     EFWGYGKDENLDYAALIAEQYRGIRPAPGYPACPDHTEKGGLFDLLNAKRAGIELTES
                     FAMYPASSVSGFYFSHPRSQYFAVGKLGRDQIGDYARRKGIAVGEAERWLAPYLGYEP
                     GQRDE"
BASE COUNT          903 a         1896 c         1804 g         1048 t
ORIGIN      
        1 gccgctcgcc accgcgggcg cgggcgtcgc gccgtccatc ccctcgcccg aaggcagctt
       61 gaattgcccg gccggatact gcttcaggaa ctccagcacc tcggcgctcg ggcggacgtc
      121 ggccatcttc gcgttcatgt cgcacaggtt gaccgcgtgg gaagcctgcg agcggtcgaa
      181 gcaggcgtcc tcgacgacgg tgacgcggta attgttggag aaggcctcgg tcaccgtcgc
      241 ccgcacgcag ccgctggtgg tggtgccagt cacgatcagg ctgtcgcagc cgagcagcac
      301 gagatacgac tgcagcggcg tgccgaaaaa cccgctcggc ttttccttcc tcacgacgat
      361 atcccgcggc gcgggcgcta tgtccgggat gatcgcgttg ccatccacgt tccgggggcg
      421 ccggaccgcg gaatcctcgc ccgcgcggtc gttgatccag agccagctgc cgcggttcca
      481 caggtccgct cgcgcggtac ccgtggtgta gatcaccggc acccccttcc cgcgtgccgc
      541 ctcgagcagg cgcccgatga ccttgatggc cggccacgcg tcctcgccgc acgagttgcg
      601 ccagcgcttg atggattcga ggatcggctc cggccggtcg ccgcagaaaa agtaattgac
      661 gtctatcacc atcagcgccg ggcgcttgcc gtagccctgc agcgccccgt agccggcggc
      721 cgccatgacc tgccggtccc gttcggtgag gaaatcatcc cagatgcgtt tggccattgt
      781 ggtgtctccg tgtgaaagca gtcctcgtca ttgccgctgg atcttcgcct tgtcgaccac
      841 gccgttccac ttggcgatct cggcggccag caggtcgcgc aggccttccg gcgtgctcgg
      901 gcgcgcttcg atgccgagtt cctgcagtcg cgccttcact tccgggatgg cgagggcggc
      961 gttgacttcc ctgttcaggc ggtcgatcac ccgtcggggc gtccccgcgg gcacggccgt
     1021 tccattccat gccgacacct caaaccctgg cagccccgcc tcggccaccg tcggaatctc
     1081 gggcagaccg gcgaaccggc gcgcggaagt caccgccagc gcccgcagcg ctccgctctt
     1141 gaggtgcggc agcacgggcg agatgaactc gaacgccgct tgcgcctccc cgctccggag
     1201 cgccgagacg agcgcgggcg tggtcttgaa gggcacgatc tgagccggca atcccgtcat
     1261 ggacttgaac agctcggatg agaggtgatg gctgctaccg atgttggtga tggcgaagtt
     1321 gaagcgggcc gggttcacct tcaccgccgc gatcagcgca ggcacggact tgacgggcga
     1381 ttctccggtg acgaccagcg ccagcgcgaa aaagcccacg gtcgaaaccg gccggaaatc
     1441 ctttaccggg tcgtagggca gcgacttgaa catcgcaacc gcgagcgcgt ggcccgtcgt
     1501 gagccacatg atcgtgtggc cgtcgggctc ggctttcctg gcaagttccg tggcgacgat
     1561 gccgcccgcg cccggccggt tgtcgatgat gacctgctgc ttcagggaat cggtcagccg
     1621 ctgcgcgagc aggcgcgcgg tgatgtccgt gaccccgccc ggaccgaacg ggagcaagat
     1681 cctgatggga cgcgcggggt aggtctgcgc gctcgccgcg ccgacggcgg cgcaacacaa
     1741 caccagggcc gcgcacgcga cccgcctggc gagcgcgcgc cggtgttccc gcgaagagta
     1801 gccatcggtc atggtgccgc tcccccttca catcgagtca caattgtaac cagttccaac
     1861 cgccccctgc atccacgcgg gcaacgcggc gttaacaggc ctgcggcgac tcttcaggga
     1921 tgagggcgga gggattaaga atgagaagaa cgagatcctg gcccgtcacc cgacacccga
     1981 cacccgacac ccgacacccg acactcgaca ctcgacatta gtcactcgtc acgctgtccc
     2041 ggttcatagc cgaggtacgg cgcaagccac cgctccgctt ccccgaccgc gatgcccttg
     2101 cggcgcgcgt aatccccgat ctggtcgcgg ccgagtttgc ccaccgcgaa gtactgtgag
     2161 cgcggatgcg aaaaatagaa tccgctcacc gacgaggccg ggtacatggc aaagctttcg
     2221 gtgagctcga tgcccgcgcg cttggcattg agaaggtcga acagcccgcc tttttcggtg
     2281 tgatccgggc acgccgggta accgggcgcc gggcggatgc cgcggtactg ctccgcgatc
     2341 agcgcggcgt agtcgaggtt ctcgtccttg ccgtaacccc agaattccct gcgcacgcgc
     2401 ttgtgcagca gctcggtgaa cgcttcggcg agccggtcgg caagcgcctg cagcatgatg
     2461 cccgagtagt cgtcgtgctg cttctcgaat ttcgcgatct gcttttcgat gccgataccg
     2521 gcggtcacgg caaacgcgcc gatggtgtcc cgcacgccag aggccttcgg cgcgatgaag
     2581 tcggcgaggc acagattggg attgccggtg ggcttcacgt tctgctggcg caggttgtgc
     2641 caggtcatga gcggccgccc gcgcggttcg tcgccgtaga tctcgatgtc gtcactgttg
     2701 acgctgaccg cccggaacag cccgaacacc gcgttcgccg tgagccagcg gccttcaatg
     2761 atcttcttca gcatggcgcg gccctcggca tagacgttgc gcgccgcctc ccccaccacc
     2821 ggatcctcca ggatcgcggg aaacgggccg gacaattccc aggtctgaaa gaacgggccc
     2881 cagtcgatgt gttcggcgat ttcggcgaga tcgtagttct tgagcagctt cacgccgggc
     2941 atctcgggcc ggggcggcac ataggcggcc cagtcggtct tgaacgcatt ggcgcgcgcc
     3001 gcctccagcg tgatgagttc cggtcccttc ttgccctggt gctgggtgcg gacgcgctcg
     3061 tattcactct tgacgtcctt gagatagccc gcgcgcaggt cgtcggacag caggttgctg
     3121 cacacgctca cgcttctcga tgcgtccggc acccagatca ccgggcccga gtagttcggc
     3181 gcgatcttga ccgcggtgtg ggtgcgcgag gtcgtggcgc cgccgatcag cagcgggatc
     3241 gtgaggccct cgcgctccat ttcgcgcgcg ttgtgcgcca tctcctcgag cgagggcgtg
     3301 atcaggcccg acaacccgat gatgtcgcag cactcgtcgc gcgcgacttc gaagatcttc
     3361 ttccacggca ccattacgcc gagattgatc acctcgtagt tgttgcactg gagcacgacc
     3421 gagacgatgt tcttgccgat gtcgtgcacg tcgcccttca cggtggcgat cacgaccttc
     3481 cccttcggcc gcgcatcacc gacgcgcgcc ttctcggcct cgatgtacgg gatgagatgc
     3541 gcgaccgcct gtttcatgac gcgcgccgac ttcaccacct gcggcaggaa catcttgccc
     3601 gagccgaaca ggtcgccgac cacgttcatg ccgtccatca acgggccttc gatcacctgg
     3661 atcgggcgcc cgcctgcctt ttccagtttt tgccgcgctt cctcggtgtc ctcgacgatg
     3721 aagtccgtga tgccccgcac cagcgcatgc gtgatccgct cctcgaccgg acccttccgc
     3781 cacgcgaggt cctcgacctg ctccctgcct ttgcccttca ccgtctcggc aaacgtcacc
     3841 atgcgctccg cggcatcggg ccggcggttg aagatgatgt cctccgcgtg ctccagcagg
     3901 tctttcggga tctcgtcgta gacgccgatc tggccggcgt tgacgatcgc catggtgagt
     3961 cccgccttga tcgcgtgata gagaaacgcg gtatggatcg cctcgcgcac ccggtcgttg
     4021 ccccggaacg agaacgagat attcgacagc cccccgctca ccctggcgtg cgggaggttc
     4081 tgccggatcc agcgcgtcgc ctctatgaag tctttgccgt agttcgcatg ctcctcgatc
     4141 cccgtcgcga tggcgaagat attgggatcg atgatcacgt cttccggcgg aaagcctacc
     4201 tcattgacga ggatgtcgta ggcggtcctg gaaattccaa ttcggcgctc gagtgtgtcg
     4261 gcctggccct tctcgtcgaa ggccatcacc accaccgcgg cgccgtagcg gcgcgcgagc
     4321 ctggcatgct tgacgaactc ctccctgcct tccttcatgc tgatggagtt caccacgcac
     4381 ttcccctgca cgcacttcag tcctgcctcg atcacttccc atttcgagga atcgatcatg
     4441 accggcacgc gcgaaatgtc gggttccgat gcgatcaggc ccaagaaggt cgtcatcgcc
     4501 tgcttcgagt cgagcatcgc ctcgtccatg ttgacgtcga tcatctgcgc gccgctctcc
     4561 acctgctggc gcgcgaccga cagcgccgcc gggtagtcgc cggcgaggat caatcgggcg
     4621 aacgccttgg agccggtgac gttggtgcgc tcgccgatat tgacgaacag ggaatcgtcg
     4681 ccgatattga gcggctcgag gccggcgagg cgcgtcttgt gctcgatcac cggcaccgcc
     4741 cgcggcggca tgtccttcaa cgcctcggcg atcgcctgga tgtgcgccgg cgtggtgccg
     4801 cagcagccgc cggcgatgtt gatgaagccg ctcttcgcga aatcgagcag gaaggcggcg
     4861 gtttgctccg gcgtctcgtc gtaaccggtc ggcgcgaggg gattgggcag cccggcgttg
     4921 ggataggcga acacgggtat gtccgcgacc ccggacagct cctcgatgta aggccgcatc
     4981 agcttcgcgc cgagcgcgca gttcagcccc accgccagcg gatgggcgtg gcgcaccgag
     5041 ttccagaacg cctccggcgt ctgtccggag agcgtgcgtc ccgacgcgtc ggtgatggtg
     5101 ccggaaatca tcacgggcag acgaattcct tgctcctcga agaattcgtc gattgcaaac
     5161 agggcagcct tggcattgag cgtatcgaat atggtctcga gcaggagaat atccactcct
     5221 ccctctacga gaccgcggac agcctcgctg tacgcggtct tgagctcgtc gaaggtcacg
     5281 ttgcgaaagc ccggatcgtt gacgtcgggc gaaatcgagg cggtgcggtt ggtgggtccg
     5341 agcgcgcccg cgacgaaacg cggcttgccc ggattcttcg cggtatacgc ttcggccgcg
     5401 gcgcgcgcga gcttcgccgc ttcgacgttc aactcgcgca ccaggtgctg gaggtggtaa
     5461 tcggcctgag cgatcgaggt cgagttgaag gtgttggtct cgatgatgtc ggcgcccgcg
     5521 gcgagatact gctcgtggat ctcgcggatg acctgcggcc gcgtgagcgt gagcagatcg
     5581 ttgttgccct tcaggtcgtg cggatggtcc cgcaaccggc tgccgcggta atccgcctcc
     5641 gtcagccgat a
//
LOCUS       DBNGSG010000282         8157 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2126468, whole genome shotgun sequence.
ACCESSION   DBNGSG010000282 DBNGSG010000000
VERSION     DBNGSG010000282.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 8157)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8157)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8157
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2126468"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="AC158Q_07300"
     CDS             <1..1108
                     /locus_tag="AC158Q_07300"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3339620.1"
                     /translation="FGHADETDDFEPTTKAHPGCSVVPAALAMAEREGRSGTELLNGV
                     ALGYDLCCRLLLALGPDLVRGSHRSAEGTSSTFGAVGAAASLARLDEKGMRHALSYAA
                     QQVSGVWSWVRDAEHVEKAFDFSGMGARNGVTAAVMAQMGFSGVWDVLDGEHNVLIAL
                     STAPKPEEMINGLGSRYFVTETAIKPYSVGYPIQSPLDAFLRLHREHKLTPDNVGRIV
                     VKVPEDGARVVNDRAMPDVNCQHIIALALVEGAVSFEDSHSYERMKDPKVRAAKALVE
                     LVADKSLMDPEAPRSGRVEVTLKDGRTVSHFTRHAPGTKENPLTTEEVSAKARSLMAP
                     VLGAGPTEEIIRRVVALEAVKDVRELSPLFSRAH"
     gene            1124..2800
                     /locus_tag="AC158Q_07305"
     CDS             1124..2800
                     /locus_tag="AC158Q_07305"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR3339621.1"
                     /translation="MDTPERKPVKTVTAARALVDGLLREGIDHVFGIPGTQNLAFLDA
                     LRDTPQIRFILTRHEQGAAFMAYGFARARNAPAVVTATEGPGVTNLVTAIAAAHKGNV
                     PLISICGVQESAMRERDATQDLDQVPFMRPITKWAYSIPYPDRVQEAVRKAFRVALTE
                     PQGPTHIEACSEVLLEPVKPETTAPAAYRNTVPPVCDPAQLDAAWKLIAGAERPLFVV
                     GRGVMKENAVPAMQALADATGIPVAALQYSPDAFPTVHPLALGPLGRNAFSSANRAAP
                     KADVVVAIGAHFDVFSTMYQYGVIGESAKLVHHTAAPGQIGIVFPVALGVTGSSASFI
                     KGLAERAGQGRPRQPWVDVARARAEFDAELAAAMKPDAFPIQSQFVAHMIRKVLPENG
                     ILVVDAGNGGKHVRSYFKSYEPGTFLCIDDWASVGGSLPIALGVKLARPDRPVLCASG
                     DMGALVNIGELETAVRENIPVVYVVFNDQGLGNERAFQNEHFGGRFFAVDYRNPDLGA
                     LARVFGAHGEHVTKPGELEGAIRRAFASGKPAVVDVMIDQNTLAPVIFKQ"
     gene            complement(2896..3504)
                     /locus_tag="AC158Q_07310"
     CDS             complement(2896..3504)
                     /locus_tag="AC158Q_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3339622.1"
                     /translation="MNDSNTATDTRRFKLIPLEKLTPEQKVLADAIRSGPRAAMKSSA
                     ASKPGPLGGPFNVWLRSPGMGNMIQQLGSEIRFRSSIPSRLNEMAILITARKWTSQYE
                     WYAHHKVAMEAGLDPAIAEAIAQGRRPEKMSADERIVYDFSTGLHETQGASDAAYQAV
                     LDRFGERGIVDLIAVNGFYVLVSMCLNVDRTPLPEGEKLPLS"
     gene            3616..3855
                     /locus_tag="AC158Q_07315"
     CDS             3616..3855
                     /locus_tag="AC158Q_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339623.1"
                     /translation="MKIVLNLLVIWLGAFAGFSAAAQADKPALPDPAYCSQRDADPEK
                     CVIQNGPAPAPIVRKPSPPPTPPAPQTPVPGKPKS"
     gene            4049..4231
                     /locus_tag="AC158Q_07320"
     CDS             4049..4231
                     /locus_tag="AC158Q_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339624.1"
                     /translation="MRSLIAGLLAVTILGGCVAVPYYAESAPYGYYYYGPAYPPASIQ
                     FRYDYHHYHRGPRHYR"
     gene            complement(4288..5676)
                     /locus_tag="AC158Q_07325"
     CDS             complement(4288..5676)
                     /locus_tag="AC158Q_07325"
                     /EC_number="2.8.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0046952 - ketone body catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase"
                     /protein_id="HIR3339625.1"
                     /translation="MDKIYASPAQAVADIPDGATIAIAGFSVGHRFATSLILALRDQG
                     TKELCLVCNSLGDPGATRGQILAENKQVRKLIAAFSVRPGTPTASEDQIVAGDMEVEL
                     VPQGILVERCRAGGAGIPAFYSPTSVGTALTEGREIRDFGGKPHVLEQAIRVDYAFLR
                     GYRADRLGNVQFRGGSQNFNPSFAKAARVAIVEVDEIVEPGGIPPELVDLPGIFVARV
                     VKTVEKFNLWRRPERRPSDKPRLYNGKPGLTRAGIAKRAAALVRDGTYVNLGVGIPTM
                     VSNYLVERDVILHAENGVLGYGHMVTEEKDIDPDTYNAAGQFVSLKPGASFFDSVTSF
                     EMARGGRIDTVILGAYEVDESGSVANWSTADAKRGGIGGAMDLLSGKGDLIIVMEHAD
                     SKGRPKLRKRCTYPLTGKGCVSYVVTDLALLRWHGGRFVLDEVAPGFTPQEVLALTEM
                     EVAVGENVCPMA"
     gene            complement(5702..6061)
                     /locus_tag="AC158Q_07330"
     CDS             complement(5702..6061)
                     /locus_tag="AC158Q_07330"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3339626.1"
                     /translation="MTLKLNHLHIKTRDPEKTARFYVDTFGATIVSQSPRGGYRLDLL
                     GLHLNVTPFLDDQKREQKYGMEHLAIDTDELDTLVEKLEAQGIPILEKTVVSGGRRVC
                     FFEGPDGVQLEFIEMKK"
     gene            complement(6170..6616)
                     /locus_tag="AC158Q_07335"
     CDS             complement(6170..6616)
                     /locus_tag="AC158Q_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339627.1"
                     /translation="MDYLLAAVAVAVAVVLVLAFVYLRRGAGHPQIGPLDVSGRVERS
                     AEEFHRDFYADLASTTIPDIRAVLEAISRATGLPAGKLEPSDPIGAIAAANGLKRAFL
                     VAFLVNEFPEAQGEILQKAAAGEFVCLHHAIALVVNARIAARAGQS"
     gene            6737..7354
                     /locus_tag="AC158Q_07340"
     CDS             6737..7354
                     /locus_tag="AC158Q_07340"
                     /inference="COORDINATES: protein motif:HMM:NF025850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HIR3339628.1"
                     /translation="MKLFGTPGSPFARKVRIVLEEKRIAHEYIVERGSAPGSRVPQFN
                     PLAKVPVLALDDGRALYDSPVIVEYLDSLGSGPRLIPDAFDERIEVKRWEALGDGIAE
                     ATVAINHDLREPQEKQHAPAWHERQRLKIDRGLAVMEKDLGAGEFCFGGRFTLADIAA
                     GYALEYLDFALPDIEWRKAHPALARLAARLAARKSFSGTQHVKSA"
     gene            7628..>8157
                     /locus_tag="AC158Q_07345"
     CDS             7628..>8157
                     /locus_tag="AC158Q_07345"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HIR3339629.1"
                     /translation="MAFALPSLPYATNALEPHISRRTMEYHHGKHHETYVANLNRLIE
                     GKPYAGMTLEEIIRRSASKSEDRAIFNNAAQAWNHTAFWNSLKPGGGGEPGGEIAVRL
                     HEAFGSYGGFREAFSAAAVGQFGSGWAWLVLNGRRLAVISTPDAVNPLVSGQAPLLAC
                     DVWEHAYYLDYQNRRKA"
BASE COUNT         1364 a         2792 c         2706 g         1295 t
ORIGIN      
        1 gttcggccac gccgatgaga ctgacgactt cgagccgacc acgaaggcgc atccgggctg
       61 ctcggtcgtg cccgccgcgc tggcgatggc cgagcgcgag gggcgctcgg ggacggagct
      121 gctcaacggc gtggcgctcg gctacgacct gtgctgccgc ctgctgctcg cgctcgggcc
      181 cgacctcgtg cgcggcagtc accgcagcgc cgagggcacc agctccacgt tcggcgccgt
      241 cggcgccgcc gcgtcgctcg cccgcctcga cgagaagggc atgcgccacg cgctctccta
      301 cgccgcgcag caggtgtcgg gcgtgtggag ctgggtgcgc gacgccgagc acgtcgagaa
      361 ggccttcgac ttctccggca tgggcgcgcg caacggcgtc accgcggccg tgatggcgca
      421 gatgggcttt tccggcgtgt gggacgtgct cgacggcgag cacaacgtgc tgatcgcgct
      481 ctccaccgcg ccgaagccgg aggaaatgat caatggcctg ggcagccgct acttcgtcac
      541 cgagaccgcg atcaagccct actccgtcgg ctacccgatc cagtcgccgc tcgacgcgtt
      601 cctgcggctc caccgcgagc acaagctcac gcccgacaac gtcgggcgca tcgtcgtgaa
      661 ggtgccggag gacggcgcgc gcgtcgtcaa cgaccgcgcc atgcccgacg tgaactgcca
      721 gcacatcatc gcgctcgcgc tggtggaggg cgccgtctcg ttcgaggaca gccactccta
      781 cgagcggatg aaggacccga aagtgcgcgc cgcgaaagcg ctggtcgagc tcgtcgccga
      841 caagagcctg atggaccccg aggcgccacg cagcggccgg gtggaagtca cgctcaagga
      901 cggccgcacc gtcagccact tcacccgcca cgcgccgggc accaaggaga atccgctcac
      961 cacggaagaa gtgagcgcca aggcgcgctc gctgatggcg ccggtgctgg gcgcgggccc
     1021 gaccgaggaa atcatccggc gggtggtcgc gctggaagcg gtgaaggacg tgcgcgagct
     1081 gagcccgctc ttttcccgag cgcactgaac agaggagcgt cacatggata cccccgagcg
     1141 caagcccgtc aagaccgtca ccgccgcgcg ggcgctggtc gacggactgc tgcgcgaagg
     1201 catcgaccac gtcttcggca tacccggcac gcagaacctc gcgttcctcg acgcgctgcg
     1261 cgacacgccg caaatccgct tcatcctgac gcgccacgag cagggcgccg cgttcatggc
     1321 ctacggcttc gcgcgcgcgc gcaatgcgcc cgcggtcgtc accgccaccg agggcccggg
     1381 cgtgaccaac ctcgtcaccg cgatcgccgc cgcgcacaag ggcaacgtgc cgctgatcag
     1441 catctgcggc gtgcaggaga gcgcgatgcg cgagcgcgac gcgacccagg acctcgacca
     1501 ggtcccgttc atgcgcccga tcaccaagtg ggcctacagc atcccctacc cggaccgggt
     1561 gcaggaggcg gtgcgcaagg ctttccgcgt ggcgctcacc gagccgcagg gaccgacgca
     1621 tatcgaggcc tgcagcgaag tgctgctgga gccggtgaag cccgagacga ccgcccccgc
     1681 cgcctaccgc aataccgtgc ccccggtgtg cgacccggcg cagctcgacg cggcctggaa
     1741 gttgatcgct ggcgccgagc gcccgctgtt cgtggtcggc cgcggcgtca tgaaggagaa
     1801 tgccgtcccc gcgatgcagg ccctcgcgga cgcgaccggc attccggtcg ccgcgctgca
     1861 gtattcgccc gacgcgttcc cgaccgtgca tccgcttgcg ctgggaccct tgggccgcaa
     1921 cgccttctcc agcgccaatc gcgccgcgcc gaaagcggac gtcgtcgtcg ccatcggcgc
     1981 gcatttcgac gtgttctcga ccatgtacca atacggcgtt atcggcgaat ccgcgaaact
     2041 ggttcaccac accgccgccc ccggccagat cggcatcgtg ttcccggtgg cgctcggcgt
     2101 gaccggctcc tcggcgagct tcatcaaggg cctcgccgag cgcgccggcc agggacgccc
     2161 gcgccagccc tgggtggacg tcgccagggc gcgcgccgaa ttcgacgccg aactcgcggc
     2221 cgcgatgaag cccgacgcgt tcccgatcca gtcgcagttc gtcgcgcaca tgatccgcaa
     2281 ggtgctgccg gagaacggca tcctcgtcgt ggacgccggc aacggcggca agcacgtgag
     2341 aagctacttc aagagctacg agcccggcac ctttctctgc atcgacgatt gggcttccgt
     2401 cggcggctcg ctgccgatcg cgctgggcgt gaagctcgcg cggcccgacc gaccggtgct
     2461 gtgcgcctca ggcgacatgg gcgcgctggt gaacatcggc gagctggaaa ccgccgtgcg
     2521 cgagaacatc ccggtggtgt acgtggtgtt caacgaccag ggcctcggca acgagcgcgc
     2581 gttccagaac gagcacttcg gcgggcgctt cttcgccgtg gattacagga acccggatct
     2641 tggcgcgctg gcgagagtat tcggcgcgca cggcgagcac gtcacgaagc ccggcgagct
     2701 cgagggcgcg atccggcgcg cgttcgcgag cggcaagccc gccgtcgtcg acgtgatgat
     2761 cgaccagaac acgctcgcgc cggtgatctt caaacagtaa ggcgaaaggc gaaaggcgaa
     2821 agtcgaaagg cgaaagtgat tctgccttac gggtgttact tccgacttcc gactttcgac
     2881 ttccgacttt cgacttcagc tgagcggcag cttctctccc tccggcagcg gcgtgcggtc
     2941 cacgttcagg cacatcgaca ccagcacgta gaacccgttc accgcgatga ggtcgacgat
     3001 gccgcgctcg ccgaagcggt ccagcacggc ctggtaagcg gcgtcgctgg cgccctgcgt
     3061 ttcgtgcagc ccggtcgaga agtcgtagac gattctctcg tccgccgaca tcttttccgg
     3121 gcggcggccc tgcgcgatgg cctccgcgat cgcggggtcg agcccggcct ccatcgcgac
     3181 cttgtgatgc gcgtaccact cgtactgaga cgtccacttg cgcgccgtga tcaggatcgc
     3241 catttcgttg agccgggagg ggatcgaact gcggaagcgg atctcggagc cgagctgctg
     3301 aatcatgttg cccatgcccg ggctgcgcag ccagacgttg aacggcccgc ccagcgggcc
     3361 cggtttcgag gcggcggagc tcttcatcgc ggcgcgcggc cccgagcgga tcgcatccgc
     3421 gagcactttc tgctccggcg tcagcttctc cagcgggatc agcttgaacc ggcgcgtatc
     3481 cgtggcggtg ttcgaatcat tcatggcgat tcccgaagtt ggaaaacgct gaacttacca
     3541 tgaagcaagg cgaaaggccg gaaggcctcg ctatcctgcc gggaacgcaa tatcccggca
     3601 tgggtcaaac gggatatgaa gatagtcctg aacctgctgg tgatctggct tggcgccttc
     3661 gcgggctttt ccgccgccgc gcaagcggac aagcctgcgt tgcccgaccc cgcgtactgc
     3721 tcgcagcgcg acgccgaccc ggaaaaatgc gtgatccaga acgggccggc tcccgcgccg
     3781 atcgtgcgca agccgtcgcc gccgccgaca cccccggcgc cgcagacgcc ggttccggga
     3841 aaaccgaaat cctgaaggcg aaagtaacga ccgttcggca ggaaacctgc cgcctttcgc
     3901 caatggcgac cgtcgttgcc gggcgacact aaacgcctgc tcccggttac aaacgtttac
     3961 accttttcgg gcactgccgg catctacgcg ggcctccgct tgtcgtacag acaggacgac
     4021 tctcgaagtc ctgatgaagg aggcgcttat gagaagcctg atagccggct tgctggcggt
     4081 cacgatactg ggcggctgcg tggcagtgcc gtattacgcg gagtccgccc cctacggcta
     4141 ttactactac gggccggcct acccgcccgc gagcatccag ttccgctacg actaccatca
     4201 ctaccatcgg ggcccgaggc actaccggtg atgcagtgat gtggctcccg cgccacggga
     4261 gtcgttttta acgcctgggg ttttccctca cgccatgggg cagacgttct cccccaccgc
     4321 gacctccatc tcggtgagcg ccagcacttc ctgcggggtg aaccccggcg ccacttcgtc
     4381 gagaacgaag cggccgccgt gccagcgcag cagcgcgagg tcggtcacga cgtagctgac
     4441 gcagcctttg cccgtcaggg ggtaagtgca gcgcttgcgc agcttggggc ggcccttgct
     4501 gtcggcgtgc tccatgacga tgatgaggtc gcccttgccc gagagcagat ccatcgcgcc
     4561 gccgatgccg ccgcgcttcg cgtccgccgt gctccagttg gcgacgctgc cggactcatc
     4621 cacctcgtag gcgccgagga tcacggtgtc gatccgcccg ccgcgcgcca tctcgaagga
     4681 agtcacgctg tcgaagaaag aggcgccggg cttcagggag acaaactggc cggcggcgtt
     4741 gtaggtgtcc gggtcgatgt ccttctcctc cgtgaccatg tgcccgtagc cgaggacgcc
     4801 gttctcggcg tggaggatga cgtcgcgctc gacgaggtag ttcgacacca tggtgggaat
     4861 gccgacgccc aggttgacgt aggtgccgtc ccggaccagc gcggccgcgc gtttcgcgat
     4921 gccggcgcgc gtcaaccccg gcttgccgtt gtagagccgc ggcttgtcgg acggccgccg
     4981 ctccggtcgg cgccagagat tgaatttctc caccgttttc acgacgcgcg cgacgaagat
     5041 gcccgggaga tcgacgagct ccggcgggat cccgcccggc tccacgatct cgtccacctc
     5101 gacgatggcg acgcgcgccg ccttggcgaa gctgggattg aagttctggc tgccgccgcg
     5161 gaactggacg ttgcccaggc ggtcggcgcg atagccgcgc aggaacgcgt agtccacgcg
     5221 gatcgcctgc tcgaggacgt ggggcttgcc gccgaagtcg cggatttccc tgccctcggt
     5281 cagcgccgtg ccgacgctgg tcggcgaata gaacgccggt atccccgccc cgcccgcgcg
     5341 gcagcgctcg accaggatgc cctgcggcac cagctccact tccatgtccc cggcgacgat
     5401 ctggtcttcg ctcgcggtgg gcgtgccggg ccgcacggaa aaggccgcga tcagctttct
     5461 cacctgcttg ttctccgcga ggatctgccc gcgcgtcgcg ccggggtcgc ccagggaatt
     5521 gcagacgagg cacagctcct tcgtgccctg gtcgcgcagg gcgaggatca ggctggtcgc
     5581 gaagcggtgc ccgacgctga atccggcgat cgcgatcgtc gccccgtccg ggatgtccgc
     5641 gacggcctgc gccggcgatg cgtagatctt gtccatccct tatcccctat cccttaccac
     5701 gctatttctt catttcaatg aactcgagct gcacgccgtc cggtccctcg aagaagcaca
     5761 cgcgccggcc gccggagacg acggtcttct cgaggatggg gatgccctgg gcttcgagct
     5821 tctccacgag cgtgtcgagc tcatcggtgt cgatggcaag atgctccatg ccgtacttct
     5881 gctcgcgctt ctggtcatcg aggaaaggcg tgacgttgag gtgcagtccg agcaggtcca
     5941 ggcggtagcc gccgcgcggg gactggctca cgatcgtcgc gccgaaggtg tcgacgtaga
     6001 accgggccgt cttctccgga tcccgggtct tgatatgcag gtggttcagc ttgagcgtca
     6061 tgggactccc attcagccgg tggcgtttcg ggaacaggca atttactcca aagcgcgcgg
     6121 caacagtaag cggggaagcg caagcgcggt ccggctcccg gctgaacgat cacgactggc
     6181 cggcccgcgc ggcgatccgc gcgttcacca ccagcgcgat ggcgtgatgc aggcagacga
     6241 actcgccggc cgccgctttc tgcaggatct cgccctgcgc ctcgggaaac tcgttgacga
     6301 ggaaggccac gaggaacgcg cgcttcagcc cgttcgcggc ggcaatcgcg ccgatcgggt
     6361 ccgaaggctc gagcttgccg gcgggcaggc cggtggcgcg cgatatcgcc tcgagcaccg
     6421 cgcgtatgtc ggggatcgtc gtgctcgcga gatcggcgta gaaatcgcga tggaattcct
     6481 cggccgagcg ctccacgcgc ccgctgacgt cgagcggccc gatctgcgga tgcccggcgc
     6541 ccctgcgaag gtagacgaac gcgagcacca gcaccacggc gacggcaacg gcaacggcag
     6601 cgagcaggta atccatgatg tccccctgtc ccatttctcg aggcggcagg cccggggaat
     6661 cctatcagcc gctgcgcccg gtggccaacg caggagcccc gccgccggtt agaatccacg
     6721 aaaccaggga gaacgcatga aattattcgg cacgccgggc agcccgttcg cgcgcaaggt
     6781 gcgcatcgtg ctcgaggaga agcgcattgc ccacgagtac atcgtcgagc gcgggtcggc
     6841 gccgggctcg cgcgtgccgc agttcaatcc gctcgccaag gtaccggtgc tggcgctcga
     6901 cgacggccgc gcgctctacg attccccggt gatcgtcgag tatctcgatt cgctggggag
     6961 cggcccgcgg ctgataccgg acgcgttcga cgagcgcatc gaggtcaagc gctgggaagc
     7021 gctgggcgac ggcatcgccg aggcgacggt ggcgatcaac cacgatttgc gggagccgca
     7081 ggagaagcag cacgcgccgg cgtggcacga gcgccagcgg ttgaagatcg accgcggcct
     7141 cgccgtcatg gagaaggatc tcggcgccgg ggagttctgc ttcggcggcc gcttcaccct
     7201 ggcggacatc gccgccggct acgcgctgga gtatctcgac ttcgccctgc cggacatcga
     7261 gtggcgcaag gcccatcccg ccctcgcccg cctcgccgcg cggctcgccg cccgcaagtc
     7321 gttcagcggc acccagcacg tcaagagcgc ttgacgaaaa acttcgccgc caggcctgtc
     7381 ctgggtaatt cgaaggggac gccaggggca cgagacttgc gtccccgcgc aagcggtatc
     7441 ggttgccccg ctgcaattta gtgaacttcg ggaacgaggc ggggtcgtca ttcatacagg
     7501 cacggaccgg cgcgcgattt tgcgcgcgat cccccggccc ggaacgcgga taacgccagg
     7561 taccgcgaat tttccgctgg cggggcggcc tgcctgattg caaacccgga aaaggagacc
     7621 cgatacgatg gctttcgcac ttccatcatt accgtatgca acgaatgcgc tggagccgca
     7681 tatttcacgg cgcaccatgg aatatcacca tgggaaacat cacgaaacct acgtcgcgaa
     7741 cctgaaccgg ctcatcgagg gcaaaccgta cgccgggatg acgctcgagg aaatcattcg
     7801 caggtcggcc tcgaagtccg aagatcgcgc cattttcaac aacgccgcgc aggcctggaa
     7861 tcacaccgcc ttctggaaca gcctgaagcc gggcggaggc ggcgaacccg gaggcgagat
     7921 cgccgtgcgg ctgcatgagg ccttcggcag ctacggcggg ttccgcgagg cgttctccgc
     7981 cgccgcggtg ggccagttcg gaagcggttg ggcgtggctc gtgctgaatg gccgcaggct
     8041 cgccgtgatc agcacgccgg acgccgtgaa cccgctggtc tcgggccagg cgccgctgct
     8101 cgcgtgcgac gtgtgggagc acgcctacta tctcgattac cagaaccggc gcaaggc
//
LOCUS       DBNGSG010000283         8722 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3994154, whole genome shotgun sequence.
ACCESSION   DBNGSG010000283 DBNGSG010000000
VERSION     DBNGSG010000283.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 8722)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8722)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8722
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3994154"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..720
                     /locus_tag="AC158Q_07350"
     CDS             70..720
                     /locus_tag="AC158Q_07350"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR3339630.1"
                     /translation="MHETRLIVIRHAETLWNRDKRMQGTTDTPLSDVGLRQAQALGRR
                     FAGHGFSALYSSDLSRARDTARAIAEHAGRELIMDPRLRERRFGIFEGLLAAEILSRF
                     PEEHARFASRDPDYAVPGGESARNFSRRCVGCLAEIAEGHPGEEIVVVTHGLVLDALY
                     RAAHGLDHGMPRPVPLINASVNLFGHGHGAWRMETWGDVSHLGPGLVTQYRGVAAV"
     gene            826..1473
                     /locus_tag="AC158Q_07355"
     CDS             826..1473
                     /locus_tag="AC158Q_07355"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3339631.1"
                     /translation="MTAAQETVVLVHGLWVHGIAMELMRRRVARSGYRVLAYSYPTVR
                     LTLAENAERFARYCRELAAPRLHFVGHSLGGLIVLRMLERATSQEPGRIVLMGVPVAA
                     SLAAQRLARLPGGRAALGRSMAEWLESGQPAAGGGRDIGVIAGRLPVGLGRIVAPDLP
                     PPNDGAVSVAETVLPGARDNILLDVSHSGMLVSRAVARQICAFLRDGAFERRDEG"
     gene            1484..2590
                     /locus_tag="AC158Q_07360"
     CDS             1484..2590
                     /locus_tag="AC158Q_07360"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008453014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIR3339632.1"
                     /translation="MRAGEKRFVTRHCVTASLRLAIAAAVLLLASGCTNLGYYWQSVN
                     GQLDVWRRERPIEEVIADPSTARALKSRLARVLEIRAFASRELGLPDNQSYRRYADLG
                     RPFVVWNVFAAPEFSVQPEQWCFVFAGCVSYRGYFAREDAERFAAGLAAGGYDVFVGG
                     VPAYSTLGWFADPVLNTVISYSGPRLARLLFHELSHQVVYVRGDTVFNESFAVAVEKE
                     GVRRWLASRGSPQDKAGYEAAVARQADFLRLAGTYQAKLASLYRSRMAPGAMRARKKE
                     LFGEMEREYRGLKDRWGGFAGYDRWFAQKPNNALLASVGIYSQLVPAFEALLEREGRD
                     LPRFYAAVRELAALPKEEREARMRALTPPVMSDK"
     gene            2682..3311
                     /locus_tag="AC158Q_07365"
     CDS             2682..3311
                     /locus_tag="AC158Q_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HIR3339633.1"
                     /translation="MRLTSTSFSNNRKIPAEFAFCAPDPKTHVTLSKNLNPHLAWYDV
                     PPNTKSLALICHDPDVPSKPDDVNQEGRTISAKLPRMDFYHWVLVDIPATTIQIQQGE
                     YSSGITARGKPGPESLNGSRQGLNDYTAWFASDASMEGKYFGYDGPCPPWNDEIRHRY
                     VFTLYALDFARCPVGGAFKAQEVLAAIKGHVITQATHTGVYALNPKVKL"
     gene            3465..3764
                     /locus_tag="AC158Q_07370"
     CDS             3465..3764
                     /locus_tag="AC158Q_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HIR3339634.1"
                     /translation="MNKLTAGSELQRGATTSDELELARLIVVTLNLEVRAEDIDPEAP
                     LYGEGLGLDSIDILELALAASKTYGVKLRSDDENNNRIFSSLRSLNRHIQQSREK"
     gene            3765..4424
                     /locus_tag="AC158Q_07375"
     CDS             3765..4424
                     /locus_tag="AC158Q_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COG4648 family protein"
                     /protein_id="HIR3339635.1"
                     /translation="MRSAIGWAWKALIVLAFIAYQYGVYLSVSNARAGLLHTLLMWLP
                     LAVLAGWILARSGNKPLWLTAVLAAGMFVFLAEHEERLGLVAVNGMSHAAVYLFLLWY
                     FGRTLARGREPLISRFSRTVHGALDPEMALFTRRVTIAWCLFFAIQLIVSALLLAFAP
                     LGAWALFVNLLNLPLLALMFVGQIAYRVIRYPDQPRTSLWQAIQVFIKDTSPSKSAEV
                     R"
     gene            4434..5789
                     /locus_tag="AC158Q_07380"
     CDS             4434..5789
                     /locus_tag="AC158Q_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3339636.1"
                     /translation="MASAPLVRGFGAGAAFAYRRGRRIGVEQFLGDVSRLAALLPERQ
                     YLLNLCADRYHFCVGFAAALVRRQVNLLPPNETPDLIERLVARYPGTYCLSDGPTGLA
                     SLETMLFPEPDGHGAQILPVPAVPVPAVPETQIAAIVFTSGTTGEPVPYRKSWGSLVR
                     VARAEIEILGLDARPGMVVVGTVPSQHVFGLEATVLQVMQGGLALYARRPFYPADVRV
                     ELASLPRPRSLVTTPVHLRVLLAEPDDLPPVDFLLCATAPLSPQLAAAAEARFAAPLH
                     EIYGCTESGGIASRRTVETEEWRAMRNVKLRAGGKRTWVKGGHVEFEVPLADIIELRS
                     RGKFVLHGRTADLVNIAGKRTSLAHLNYHLNSIEGVRDGAFVLPEQEGAAVTRLTAYV
                     VAPALTPSALMDALRLRIDAAFMPRPLHFVDALPRNDVGKLPRQSLSEFGTELAGRVG
                     G"
     gene            5791..7143
                     /locus_tag="AC158Q_07385"
     CDS             5791..7143
                     /locus_tag="AC158Q_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3339637.1"
                     /translation="MPTATIPLVHGYERLSVFAYRKGRPVGLDDFLRDAARLAARLPR
                     RRHVLNLCADRYRFAVGFAAALLREQVSLLPPNDTPDLIERLVAQYPDVYCLTDGPAG
                     PAALETVSFAESPNAGGSEAPAVPAFPDSQMAAILFTSGSTGEPVPYPKTWGSLARSA
                     RAEIDRLGVRALPGMAVLGTVPPQHSYGLESTVLMVMQGGLAMHAGRPFYPADIRADL
                     ASLPRPRCLVTTPVHLRVLLAEPDELPPADFLLCATAPLSPQLAAQAEARFHAPLYEI
                     YGCTEAGQIAARRTVKSSEWRALAGIKLRAHGKGTWVSGGHVETEVLLADIIEMRGRE
                     KFLLHGRTADLVNVAGKRTSLAHLNYQLNSIEGVLDGVFIVPAEKGESVVRLTAFVVA
                     PGHTSSTLMEALRQRIDPVFLPRPLCFVDVLPRNATGKLPRQPLDRIAAGFGSSTEPG
                     "
     gene            7140..7451
                     /locus_tag="AC158Q_07390"
     CDS             7140..7451
                     /locus_tag="AC158Q_07390"
                     /inference="COORDINATES: protein motif:HMM:NF047268.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApeI family dehydratase"
                     /protein_id="HIR3339638.1"
                     /translation="MTVIEKVFPLDHPASNGHFPGNPIIPGAVLLSETAQAIAAGLDS
                     AFALYQVRSAKFVHPVRPGDRVLIQFSRDDRGEVRFTCAVEGRPVLTGQMACSAPPTA
                     K"
     gene            7424..8344
                     /locus_tag="AC158Q_07395"
     CDS             7424..8344
                     /locus_tag="AC158Q_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family acyltransferase"
                     /protein_id="HIR3339639.1"
                     /translation="MLRPADSEVNESQAPEWMTAPERSTMLALRSIAWVALALGRPAA
                     RLLLYPISLYFVLFSRRPRTASRQYLSKVLGRKAGMADTFRHYHTFAAVILDRLYLLK
                     DQISRFEVHVHGAEIVEELIAGGEGCVLLGAHMGSFEIIRTAGRARGFKVSMVMYEHN
                     ARKIRKLLEAINPALHMEVIGLGSVDSMLKVEAALARGEIVGLLADRTIQGGGTVSCP
                     FLGEQAAFPIGPVRIAAMLKRPIALMFGIYRGGNRYEVHFERLAGLDRIDGRERDAMT
                     AQVLRRFAERLEHYCRIAPYNWFNFYDFWR"
     gene            8328..>8722
                     /locus_tag="AC158Q_07400"
     CDS             8328..>8722
                     /locus_tag="AC158Q_07400"
                     /inference="COORDINATES: protein motif:HMM:NF039988.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LolA family protein"
                     /protein_id="HIR3339640.1"
                     /translation="MISGGSPGTRRRRAVLALLAAGFCSPRAIAAEASEPGAWDVNRL
                     MRELGQVKSSRARFVESKHIAILSAPLESTGTLLYTAPGRLEKHTQTPRRESLVLEGD
                     RLTLENKDRNQRRTFALQDQPVIWAFVES"
BASE COUNT         1427 a         2789 c         2932 g         1574 t
ORIGIN      
        1 gtccaataat gacgcatcac gggccgataa tgatccattg cccttcagac acgattgctg
       61 aaacgaacca tgcatgaaac cagattgatc gtgattcgtc atgccgaaac cctctggaat
      121 cgcgacaagc gcatgcaggg cacgaccgat acgccgctct cggacgtggg cctccgccag
      181 gcgcaggcgc tcggccgccg gttcgccggg catggattca gcgcgctcta cagcagcgac
      241 ctgtcccggg ccagggacac ggcccgcgcg atcgcggagc acgcgggccg cgagctcatt
      301 atggatccgc ggctgcggga gcgccggttc gggatcttcg agggactgct cgccgccgag
      361 atcctgtctc gctttccgga ggagcacgcc cgcttcgcca gccgcgaccc ggactacgcc
      421 gtgcccggcg gcgagagcgc gcgcaacttt tcgcggcgct gcgtcggttg cctcgccgag
      481 atcgccgaag ggcaccccgg agaggagatc gtggtcgtca cccacgggtt ggtgctcgat
      541 gcgctctatc gcgcggcgca cggcctcgac cacggcatgc cgcggccggt gccgctgatc
      601 aacgcgagcg tgaatctctt cggccacggc catggcgcct ggcggatgga aacgtggggc
      661 gacgtgagcc atctcggccc cgggctggtc acgcagtatc gcggcgttgc cgctgtctga
      721 aaaagtgact cgtttctcgt tactcgtttt tcgtttttcg cgaagcgcgc tcattgcttc
      781 gaccgggaac gagaaacgag caacgagcca ctctcccggc cgtcgatgac ggctgcccag
      841 gaaaccgtcg tcctggtgca cggcctgtgg gtgcacggaa tcgcgatgga gctgatgcgg
      901 cggcgcgtgg cgcgctccgg ctatcgtgtg ctcgcctact cctatcccac cgtgcggctc
      961 acgctcgccg agaacgccga gcggttcgcc cgttactgcc gcgagctggc ggcgccgcgg
     1021 ctgcacttcg tcgggcacag cctgggtggg ctcatcgtgc tgcgcatgct cgagcgtgcg
     1081 accagccagg aacccggacg catcgtgctc atgggcgtcc cggtcgcggc cagcctggcc
     1141 gcgcagcgtc tcgcccgcct gccgggaggc cgcgcggcgc tggggcgcag catggcggaa
     1201 tggctggaat ccggacaacc ggcggccggc ggcggccgcg acatcggcgt catcgcgggc
     1261 cggctgccgg tgggtcttgg gcgaatcgtt gcgccggacc tgccgccgcc gaacgacggc
     1321 gcggtcagcg tcgccgaaac ggtgttgcca ggcgcccgcg ataacatcct gctggacgtg
     1381 agccacagcg gcatgctcgt ctcgcgcgcc gtcgcccggc agatttgcgc gtttctgcgc
     1441 gacggcgcgt tcgaacgccg ggatgaggga tgagggcgga gggatgaggg ccggagaaaa
     1501 gcggtttgtc acccgtcact gcgtcaccgc gtcactgcga ctcgccattg cagcagcagt
     1561 actgctgttg gcgagcggct gcacgaacct cggctactac tggcagtcgg tcaacgggca
     1621 actcgatgtc tggcgccgcg aacggccgat cgaggaggta atcgccgatc cgtccactgc
     1681 ccgggcgctg aaaagcaggc tcgcgcgcgt gctggagatt cgggcgttcg cgagccgcga
     1741 gctgggactc cccgataacc aaagctaccg ccgctacgcc gatctcggac ggccgttcgt
     1801 ggtgtggaac gtgttcgcgg cgccggagtt ctcggtacag cctgaacagt ggtgcttcgt
     1861 gttcgccggt tgcgtttcgt accgcggcta cttcgccagg gaggatgccg agcgtttcgc
     1921 cgccggactc gccgcagggg gatacgatgt ctttgtgggt ggcgtgccgg cttactcgac
     1981 gctgggatgg tttgccgatc ccgtgctcaa cacggtgatc agctattcgg gcccgcggct
     2041 cgcgcggctg ctgtttcacg agctttccca tcaggtggtt tacgtgcgcg gcgataccgt
     2101 gttcaacgag tcgttcgcgg tcgcggtcga aaaggagggc gtgaggcgct ggctcgcgag
     2161 ccggggaagc ccgcaggaca aggcgggtta cgaagccgcg gtcgcgcggc aggccgattt
     2221 cctgcgactg gccgggacct accaggcgaa gctcgcatcg ctgtaccgga gccggatggc
     2281 gcccggggcg atgcgcgcgc gcaagaagga attgttcggg gaaatggaac gggagtaccg
     2341 cggcctcaag gacaggtggg gaggatttgc gggctacgac cgctggttcg cgcaaaaacc
     2401 caacaacgcc ctgcttgcct cggtcggcat ctactcgcag ctcgtgccgg ctttcgaggc
     2461 gttgctcgag cgcgagggcc gcgatctccc gcgcttttat gcggcggtca gggaactggc
     2521 tgccttgcct aaggaggagc gggaggcgcg tatgcgcgct ctcacgcctc ctgtgatgag
     2581 tgacaagtga cgagacccgg cctgatcccg aatcccgatc ggcgcggcaa cgcgccgatg
     2641 ctttgatata attttttgca gtattgatcg aggaggcgat catgaggctc accagcacca
     2701 gcttctccaa caaccggaaa attcccgccg agttcgcgtt ttgcgcgccg gatcccaaga
     2761 cccacgtgac gctttcgaag aatctcaatc cgcacctggc gtggtatgac gtgccgccga
     2821 acaccaagtc gctcgcgctg atctgccacg atcccgacgt gccgagcaag ccggacgacg
     2881 tgaaccagga aggccgcacc atttccgcga aactgccgcg catggacttc tatcactggg
     2941 tgctggtgga cattccggcg acgacgatac agatccagca gggcgagtac tcgagcggca
     3001 tcacagcgcg cggcaaaccc gggccggaga gcctgaacgg ctcgcgccag gggctgaacg
     3061 actacaccgc gtggtttgcc tcggacgcgt ccatggaggg caagtacttc ggctatgacg
     3121 gcccgtgccc gccgtggaac gacgaaatcc ggcatcgcta cgtgttcacg ctctacgcgc
     3181 tcgactttgc ccggtgcccc gtgggcgggg ccttcaaggc gcaggaagtg ctcgcggcga
     3241 tcaagggcca cgtcatcacg caggcgacgc acaccggcgt ctacgccctg aatccgaaag
     3301 tcaagcttta agtccgttac ccggcttgat ttgagggggg attctgacgg gccgcacgcc
     3361 cgtgacccct ggcctggcca aaataaacaa tgtcgtctac cgcacataag ggtcccgaca
     3421 atgtcacaat tacgactgcc tggagaatcc cggaggattg cgccatgaac aagctcacgg
     3481 ccggcagcga attgcagcgc ggggccacga catccgatga gctcgagctg gcccggctga
     3541 tcgtggttac gctcaacctg gaagtgcgag cggaggacat cgacccggaa gccccgctct
     3601 acggggaggg gctggggctg gattcgatcg atatcctgga gctggctctt gccgcctcca
     3661 agacctacgg cgtcaagctg cgttccgatg acgagaacaa caacaggatt ttcagttcgc
     3721 tgcgcagcct gaaccggcac atccagcagt cccgagaaaa atagatgcgc agcgcgatcg
     3781 gttgggcgtg gaaagcgctc atcgtgctcg cattcattgc gtaccaatac ggcgtttact
     3841 tgtcggtcag caacgcgcgg gcgggtctgt tgcacaccct cctgatgtgg ctgccgctgg
     3901 cggtgctggc gggatggatt cttgcgcgct caggcaacaa gccactttgg ctgacggccg
     3961 tgttggcagc agggatgttc gtttttctgg cggagcatga ggagcgtctg ggcctcgttg
     4021 cggtaaacgg catgtctcat gcggcggtct acctgtttct gttgtggtat ttcggacgca
     4081 cgctggcgcg cggaagagag ccgctcatca gccggttttc gcgcacggtg cacggcgcgc
     4141 tggatccgga gatggcgctg ttcacgcgca gggtcacaat cgcctggtgc ctgttcttcg
     4201 ccatccagtt gatcgtctcg gcgctgttgc tcgcgttcgc ccctctcggc gcctgggcgc
     4261 tgttcgtcaa tctcctcaat ttgccattgt tggcactgat gttcgtgggg caaattgctt
     4321 accgggtaat ccgctacccc gaccagccgc gaacttccct gtggcaggcg atccaggtgt
     4381 tcatcaagga cacatcgccc tcaaagagcg cggaagtgcg atagcataat cccatggctt
     4441 cggcaccgct cgttcgcgga tttggcgcag gcgcagcatt cgcatatcgc cgggggcgcc
     4501 gcatcggcgt cgagcaattc ctgggagacg tgtcgcgcct tgccgctttg cttcccgagc
     4561 ggcagtacct gctgaacctc tgcgcggacc gatatcattt ttgtgtcggg tttgcggcgg
     4621 cgctggtgcg aaggcaggtg aacctgttgc cgcccaacga gacgcccgac ctgatcgagc
     4681 gcctggttgc ccggtatccc gggacctact gcctgagcga cgggccgacg ggcctggcgt
     4741 cgctcgaaac gatgcttttt cccgagcccg atggccacgg cgcgcaaatc ctcccggttc
     4801 ccgctgtgcc agtccccgcg gtgccggaaa cccagattgc ggcaattgta ttcacatcgg
     4861 gcacaaccgg cgaaccggtg ccctaccgca agagctgggg cagtctcgtc cgggtcgcgc
     4921 gcgcggagat cgagatactc gggctggacg cgcgaccggg catggtggtc gttggcaccg
     4981 tgccgtcgca gcacgtgttc gggctggaag ccaccgtgct gcaggtcatg cagggcggcc
     5041 tggcattgta cgcgcggcgg ccgttctatc cggccgacgt ccgtgtggaa ctggcgtcgt
     5101 tgccgcggcc gcgcagcctg gtgactacgc cggtacattt gcgcgtgctg ctggcggaac
     5161 ccgacgacct gcccccggtc gattttctgc tgtgcgccac ggcgccgttg tcgccgcaac
     5221 tggctgctgc ggcggaagcg cgtttcgccg cgccgctgca cgagatctac ggttgcactg
     5281 aatcaggggg aatcgccagt cgccgcacgg tggaaaccga ggaatggcgc gcgatgagga
     5341 atgtgaagct gcgcgccggc ggcaagcgga cgtgggtcaa gggcggccac gtcgagttcg
     5401 aggtgccgct ggccgacatc atcgaattgc ggagccgggg aaaattcgtt ctgcacggac
     5461 gcaccgctga cctggtgaat atcgccggca agcgcacgtc gctggcgcac ctcaactacc
     5521 atctgaactc gatcgaaggc gtgcgcgacg gcgcattcgt gctgcccgaa caggagggcg
     5581 cggcagtgac ccggcttacg gcatacgtgg tggcgccggc gctcaccccc tcggcgttga
     5641 tggacgcgct gcgtctgcgc atcgatgccg catttatgcc ccggccgctg catttcgtgg
     5701 acgccttgcc gcgcaacgat gtgggaaagc tgccgcgcca gtcgctcagc gaattcggga
     5761 ccgaattggc cggacgagtg gggggctaac gtgccaacgg ccacaatccc cctggttcac
     5821 ggatacgagc gcctttcggt gtttgcctat cgcaaagggc gccccgttgg tctcgacgat
     5881 tttctgcgcg acgcggcacg gctggcggcg cggctgccgc ggcgacggca tgtgctcaac
     5941 ctctgtgccg accgctatcg gttcgccgtc ggctttgccg cggcgctgct gcgggaacag
     6001 gtcagcctgc tgccgcccaa cgacacgccc gatctcatcg agcggctggt cgctcaatac
     6061 cctgatgtct attgcctgac ggacggcccc gcggggcccg ctgcgctcga gacggtgtct
     6121 tttgcagagt cccccaacgc cggcgggtcc gaagcgccgg ccgtgccggc tttcccggat
     6181 tcgcaaatgg ccgccatcct gttcacttcg ggctccaccg gcgagccggt gccttatccc
     6241 aagacgtggg ggagcctcgc gcgcagcgcc cgcgctgaaa tcgaccgtct cggggtgcgc
     6301 gcgctgcccg gcatggccgt actcggcacc gtcccgccgc agcactctta tggcctggaa
     6361 tccaccgtgc tgatggtcat gcagggcggg ctggccatgc acgccgggcg gccgttttac
     6421 ccggccgaca tacgcgcgga cctggcgtcg ttgccgcggc cgcgctgcct ggtgaccaca
     6481 ccggtgcacc tgcgcgtgct gctcgcggag ccggacgagc tgccgcccgc tgatttcctg
     6541 ctgtgcgcga cggcgccgtt gtcgccgcag ctcgcggcgc aggcggaagc gcgcttccac
     6601 gcgcccctct acgaaatcta cggctgtacc gaggccggcc agatcgccgc gcggcgcact
     6661 gtcaagtcca gcgaatggcg cgcattggcc gggatcaagt tgcgcgccca tggcaaaggc
     6721 acctgggtca gtggcggaca cgtcgagacg gaagtcctgc tggcggacat catcgagatg
     6781 cgcggccggg agaagttcct gttgcacggc cgcacggcgg acctcgtgaa cgtcgcgggc
     6841 aagcggactt cgctcgcgca cctcaactat cagctgaact cgatcgaagg cgtgctcgac
     6901 ggagtcttta ttgttcccgc agagaagggc gagtcggtgg tgcggttgac ggcgttcgtg
     6961 gtggcgccgg ggcatacgtc ttctaccctg atggaggcgc tgcgccagcg catcgatccc
     7021 gtcttcctgc cgcgcccgct gtgcttcgtc gacgtcctgc cgcgcaacgc caccggcaag
     7081 ctgccgcgcc agccgctcga ccggatcgcg gccggattcg gatcgtcgac ggaaccggga
     7141 tgaccgtgat cgaaaaggtc ttcccgctcg atcacccggc gtcgaacggg cattttcccg
     7201 gcaatcccat catcccgggc gcggtgctgt tgagcgaaac tgcgcaagcc atcgcggcgg
     7261 gcctcgactc cgctttcgcg ctttatcagg tcaggtcggc caagttcgtg catccggtcc
     7321 ggccgggcga ccgggtcctg atccaattct cccgcgatga ccggggcgag gtccggttta
     7381 cttgcgccgt ggaggggagg ccggttctga ccggccagat ggcatgctcc gccccgccga
     7441 cagcgaagtg aatgagtcgc aggcgccgga gtggatgacg gcgccggaac gcagcactat
     7501 gctggcgttg cgttccatcg cctgggtggc gctggcgctc gggcggcccg cggcgcggct
     7561 gctgctctat cccatctcgc tctacttcgt gctgttctct cgcaggccgc gaaccgcctc
     7621 gaggcaatac ctgagcaagg tgctgggacg caaggcgggc atggcggata cgttccgcca
     7681 ctaccacacc tttgcggccg tcatactcga ccggctttat ctcctcaagg accagatctc
     7741 ccggttcgag gtgcacgtcc acggcgccga aatcgttgag gagctgattg cgggcgggga
     7801 aggctgcgtg ctgctcggcg cgcacatggg cagcttcgag atcattcgca cggccggaag
     7861 ggcgcgcggg ttcaaggtca gcatggtgat gtacgagcac aacgcgagga aaatccggaa
     7921 attgctcgaa gccatcaatc cggctttgca tatggaggtc ataggcctgg ggagcgtgga
     7981 ttccatgctc aaggtcgaag ccgcactggc gcgcggcgaa atagtgggcc tgctggcgga
     8041 ccggacgatc cagggtggag gcacggtatc ctgtcccttc ctcggcgagc aggccgcttt
     8101 cccgattggg ccggtccgga tcgccgcaat gctgaaacgg ccgatcgcgc tgatgttcgg
     8161 gatctatcgg ggcggcaatc gttatgaagt ccacttcgag cggttggcgg gcctcgaccg
     8221 gatcgacggc agggagcgcg atgcgatgac cgcacaggtg ttgcgacgtt tcgcggaacg
     8281 actggagcat tattgccgga tcgcgcccta caactggttc aatttctatg atttctggcg
     8341 gtagcccggg cacgcgccgc aggcgggcag tgctggcgct gctcgctgcc ggcttctgtt
     8401 cgccccgcgc tatcgccgcc gaggcgagtg agcctggcgc ctgggacgtc aatcggctca
     8461 tgcgcgagct gggccaggtg aaatcctcca gggcgcggtt cgtcgaaagc aaacacatcg
     8521 ccatactgag cgcgccgctg gaatcgacgg gaaccctgct ctataccgcg cccggcaggc
     8581 tcgaaaagca tacgcagacg ccccggcggg aaagcctggt gctggaaggc gaccggctga
     8641 ctctcgagaa caaggatcgc aaccagcgcc gcacctttgc gctccaggat cagcccgtga
     8701 tctgggcttt cgtcgagagc at
//
LOCUS       DBNGSG010000284         4093 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_768857, whole genome shotgun sequence.
ACCESSION   DBNGSG010000284 DBNGSG010000000
VERSION     DBNGSG010000284.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4093)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_768857"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="AC158Q_07405"
     CDS             complement(<1..752)
                     /locus_tag="AC158Q_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HIR3339641.1"
                     /translation="MRKLWLVFAQTATLCLAVLFVVSTLRPDLLAWNSRGNVVTVKEP
                     PALHAPARATASFSDAARKAMPAVVNIFTSQEVKRPRHPFTDDPLFRYFFGDQLEAGP
                     QRREGLGSGVIVSEQGYILTNHHVVESVDQIEIALSDSRKLKARVVGTDPETDLAVLK
                     VEKQKLPAITFAKSEQLRVGDVVLAIGNPFGIGQTVTLGIVSGLGRSHLGITVFENFI
                     QTDAAITPGNSGGARVDADGNLVGVNTAIYSQT"
     gene            950..1543
                     /gene="petA"
                     /locus_tag="AC158Q_07410"
     CDS             950..1543
                     /gene="petA"
                     /locus_tag="AC158Q_07410"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - quinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HIR3339642.1"
                     /translation="MADKELDQGKRRLLLAATGVAGGVAGVAAAVPFVASMLPSERAK
                     AAGAPVEADVSKLEPGEKMVVEWRGKPVWIVRRTKEMLAAAKADEVKVADPASAKPMQ
                     PDYAKNEYRSIKPEYLVLVGICTHLGCSPVDKLQAQAESFASDWKGGFYCPCHGSLFD
                     LAGRVYKNKPAPENLEVPPHKYLSDTRILIGEDSKGA"
     gene            1548..3005
                     /locus_tag="AC158Q_07415"
     CDS             1548..3005
                     /locus_tag="AC158Q_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008121 - quinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HIR3339643.1"
                     /translation="MAAAEKQPVTITCAGPLNGPLSGFLTWVDQRFPMMSLWRDHLAE
                     YYAPKNFNFWYYFGSLAMAVLVLQIVTGIFLTMHYKPDAAQAFASVEYIMRDVPGGWL
                     IRYLHSTGASAFFIVVYLHMFRGLMYGSYRQPRELIWIFGMLIYLCLMAEAFFGYLLP
                     WGQMSYWGAQVIVNLFNALPVIGPDLALWIRGDYVVSDATLNRFFAFHVIAIPLVLLG
                     LVAAHIMALHEVGSNNPDGVEIKKTKDPKTGHPVDGIPFHPYYTVKDTLGVVVFAVVF
                     CVILFFMPEMGGYFLEYNNFIPADPLKTPPHIAPVWYFTPYYSILRAITEDFMWVLAI
                     GVVAYTALIWISVRDKRIRYAAAAIGFVVIMLMKGPQVELDLGFGALKLPFEAKVWGV
                     VLMGVSTLIFFLLPWLDRSPGKSIRYKGALCKTALAIFVITFLILGYLGTLTVTEGRT
                     LAAQVFTLIYFAYFLLMPWYTRMDRTRPEPARVTW"
     gene            3044..3790
                     /locus_tag="AC158Q_07420"
     CDS             3044..3790
                     /locus_tag="AC158Q_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008115638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HIR3339644.1"
                     /translation="MLLAVPLVALAAEGVKLDRMPADVNPNDPVSLQRGAQVYVNYCL
                     GCHSVSYMRYNRLQDLGITEQQIRDNLIFTGAKVGDLMTIAMTPRDAKEWFGAPPPDL
                     SVIARSRSSHAGSGADWLYTYLRGFYRDSTRPTGWNNTVYPNVGMPHMLHALQGEQVL
                     KTEVVSIPRGNKGEVEKHEVQKLVLAKPGTLKPAEYDRLVADLVNFLSYVGEPAKQLR
                     TQLGIYVLMFLGVLFALAYALKKEYWKDVH"
     gene            3883..>4093
                     /locus_tag="AC158Q_07425"
     CDS             3883..>4093
                     /locus_tag="AC158Q_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006999756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HIR3339645.1"
                     /translation="MMTLYSGTTCPFSQRCRIVLYEKGMDFQIVDVDLFNKPEDLAVM
                     NPYNQVPVLVERDLILYQSNIINEYI"
BASE COUNT          725 a         1301 c         1291 g          776 t
ORIGIN      
        1 gtctgggagt agatcgcggt gttcacgccg accaggttgc cgtccgcgtc cacccgcgcg
       61 ccgcccgaat tgccgggagt gatcgcggcg tcggtctgga tgaaattctc gaaaaccgtg
      121 atgccaaggt ggctgcggcc gagaccgctg acgatgccca gcgtgacggt ctgcccgatg
      181 ccgaatgggt tgccgatcgc gagcacgacg tccccgacgc gcaactgttc cgacttggcg
      241 aaggtgatcg cgggcagctt ctgcttctcg accttgagca ccgcgagatc ggtttcgggg
      301 tcggtgccga ccacgcgcgc cttcaacttg cgcgagtcgg atagcgcgat ctcgatctgg
      361 tccaccgatt cgaccacgtg gtggttggtc aggatgtagc cctgctcgct gacaatcacg
      421 cccgaaccca gtccctctcg gcgctgcggc ccggcctcga gctggtcgcc aaagaaatag
      481 cggaacaggg gatcgtccgt gaacggatgg cgcgggcgtt tgacttcctg gctggtgaaa
      541 atgttgacga ccgccggcat cgccttgcgc gccgcgtcgc tgaacgaggc ggtcgcgcgg
      601 gccggcgcgt gcagcgccgg cggttccttg accgtcacga cattgccgcg cgagttccag
      661 gcgaggagat ccggccgcaa cgtcgagacc acgaacagca cggcgaggca gagcgtcgcg
      721 gtttgtgcga aaacgagcca gagcttgcgc atgggggccg atagcggggc gatgacgccg
      781 ggatagtccg tcgagtccga atcatagcaa accggctgaa aacggctttg cacggcaatc
      841 cgggatgacc gcgtaatacc atgattaaga taaataattt cacgttgcgc ttatgttttc
      901 cgggggcttt gtgtagaatg ccactctttt aggcagtgga tagaatcgta tggcggacaa
      961 agagttggat cagggcaagc ggcgcctgtt gctcgccgcc accggggtcg cgggcggcgt
     1021 cgcgggtgtc gcggcagccg tgcctttcgt cgccagcatg ctgccctcgg agcgtgccaa
     1081 ggcggcgggc gcgccggtcg aggccgatgt cagcaagctc gagccgggcg agaagatggt
     1141 cgtggaatgg cgcggcaagc cggtatggat cgtgcgccgc acgaaggaaa tgctggcggc
     1201 ggcgaaggcg gacgaagtca aggtcgccga cccggcgtcc gccaagccca tgcagccgga
     1261 ctacgcgaaa aacgagtatc gctcgatcaa gcccgagtac ctggtgctgg tcggcatctg
     1321 cactcacctg ggctgttccc cggtggacaa gctccaggcc caggccgaat cctttgcttc
     1381 ggactggaaa ggcggcttct actgtccgtg ccatggctcg ctgttcgacc tcgcgggccg
     1441 cgtctacaag aacaagccgg cgcccgagaa cctggaagtg cctccccaca agtacctctc
     1501 cgacacgcgc atcctgatcg gcgaagacag caagggagcc tgacgacatg gcggccgccg
     1561 aaaaacaacc ggtcacgatc acctgcgccg gcccgttgaa cgggccgttg agcggcttcc
     1621 tcacctgggt cgaccagcgc tttccgatga tgtcgctatg gcgcgaccac ctcgccgagt
     1681 actatgcgcc gaagaacttc aatttctggt actacttcgg ctcgctcgcg atggcggtgc
     1741 tggtgctgca gatcgtcacc ggcatcttcc tgaccatgca ctacaagccg gacgcggcgc
     1801 aggccttcgc gtccgtcgag tacatcatgc gcgacgtgcc gggcggctgg ctgatccgct
     1861 acctgcactc gaccggggcc tccgccttct tcatcgtcgt ctacctccac atgtttcgcg
     1921 ggctcatgta cggctcctac cggcagccgc gcgagttgat ctggatcttc ggcatgctga
     1981 tctacctgtg cctcatggcc gaggcgttct tcggctacct gctgccgtgg ggccagatgt
     2041 cctactgggg cgcgcaagtg atcgtcaacc tgttcaacgc gctgccggtc atcgggcccg
     2101 acctcgccct gtggatccgc ggcgactacg tggtgtcgga tgcgacgttg aaccgctttt
     2161 tcgcgttcca cgtgatcgcg atcccgctcg tgctcctcgg gctcgtcgcg gcgcacatca
     2221 tggcgctgca cgaggtagga tcgaacaacc cggacggcgt cgagatcaag aagacgaagg
     2281 acccgaagac cgggcacccg gtcgacggca tcccgttcca tccttattac acggtcaagg
     2341 ataccctggg cgtggtggtg ttcgcggtcg tgttctgcgt gatcctgttc ttcatgccgg
     2401 agatgggcgg ctatttcctc gagtacaaca acttcattcc ggccgacccg ctgaagacgc
     2461 ccccgcacat cgcgccggtg tggtacttca cgccctatta ctcgatcctg cgcgcgatca
     2521 ccgaggattt catgtgggtg ctcgcgatcg gcgttgtggc ctacacggcc ctgatctgga
     2581 tctcggttcg cgataaacgc attcgttacg cggctgcggc catcgggttt gtcgtgatca
     2641 tgctgatgaa ggggccgcag gttgaactcg atctgggatt cggcgcgctc aagcttccct
     2701 tcgaagccaa ggtatggggc gtggtgctga tgggcgtgtc caccctgata ttcttcctgc
     2761 tgccctggct cgaccgcagc ccgggcaagt ccatccgcta caagggcgcg ctgtgcaaga
     2821 ccgcgctggc gatattcgtg atcacgttcc tgatcctggg ctacctgggg acgctgaccg
     2881 tgacggaagg acgcacgctt gccgcgcagg tcttcacgct catttatttc gcctacttcc
     2941 tgctgatgcc gtggtatacc cggatggacc ggacccggcc cgaaccggcg cgggtgacgt
     3001 ggtgaggcgc gcgcgcttga cgccagcccg aaccctcgcg tggatgctgc tcgcggtgcc
     3061 gctcgtcgcg cttgccgcgg agggcgtgaa gctcgaccgc atgccggccg acgtgaaccc
     3121 gaacgacccg gtctcgctgc agcgcggcgc gcaggtctac gtcaactact gtctcggctg
     3181 tcacagcgtg tcctacatgc gctacaaccg gctgcaggac ctggggatca ccgaacagca
     3241 gatccgcgac aacctgatat tcaccggggc caaggtcggc gatctcatga cgatcgccat
     3301 gacgcccagg gacgccaagg agtggttcgg cgcgccgccg ccggacctgt ccgtgatcgc
     3361 gcgctcccgc tcatcgcacg cgggcagcgg ggcggactgg ctctacacct atctgcgcgg
     3421 cttttaccgc gattccaccc gccctaccgg ctggaacaac acggtttacc cgaacgtcgg
     3481 catgccgcac atgctccatg cgctgcaagg cgagcaggtt ttgaagaccg aggtggtgtc
     3541 cataccccgc ggcaacaagg gggaggtgga aaaacacgag gtgcagaagc tggtgctggc
     3601 gaagcccggg accctgaagc ccgcggagta cgaccgccta gtggccgatc tcgtgaactt
     3661 cctctcgtac gtgggcgagc ccgccaaaca attgcgcacg caactcggca tttacgtgct
     3721 gatgttcctg ggcgtgctgt tcgcgctcgc ctatgccctg aaaaaagaat actggaaaga
     3781 cgtccactga tgagcggtaa gcggcgggtg gagccataac tgcccgcagt cccgctgtcg
     3841 tttccccatc ctcaccgccg tcacggcccc ctcaccgctc atatgatgac cctctactcg
     3901 ggcaccacct gcccattcag ccagcgctgc cgcatcgtgc tctacgagaa gggcatggat
     3961 ttccagatcg tggatgtgga cctcttcaac aagccggaag acctggcggt gatgaatccc
     4021 tacaaccagg tgccggtgct ggtggagcgc gatctcatcc tctaccagtc gaacatcatc
     4081 aacgagtaca tcg
//
LOCUS       DBNGSG010000285         7709 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1787482, whole genome shotgun sequence.
ACCESSION   DBNGSG010000285 DBNGSG010000000
VERSION     DBNGSG010000285.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7709)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7709)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7709
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1787482"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1288)
                     /gene="ffh"
                     /locus_tag="AC158Q_07430"
     CDS             complement(<1..1288)
                     /gene="ffh"
                     /locus_tag="AC158Q_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HIR3339646.1"
                     /translation="MFDNLTGRLSRVIKTLRGEARLTESNVQEALREVRVALLEADVA
                     LPVVRAFIERVREKALGQEVVGSLTPGQAAVGVVYRELVALMGEKNDSLNLAAQPPAV
                     ILLAGLQGAGKTTTAGKLARWLKTELKKKVLLASCDVYRPAAIEQLKTLALQVEAGFF
                     PSAIGEKPLDIATRALDYARKHYADVLIVDSAGRLAIDEAMMREIGELHRALNPVETL
                     FVVDAMQGQDAVNTAKAFADALPLTGIILTKLDGDARGGAALSVREVTGKPVKFAGVG
                     EKLAALEPFHPDRMASRILGMGDVLSLIEDVQKGVDHEEAVKLAKKVKSGKDFDLEDF
                     KQQIAQMKKMGGLAALADKLPGNLGQLAQGAPGMDDKAIRRIEGIINAMTREERAKPE
                     ILKASRKRRIAAGAGVAVQEVNRLLNQFEQTQKMMKM"
     gene            1355..2164
                     /locus_tag="AC158Q_07435"
     CDS             1355..2164
                     /locus_tag="AC158Q_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C assembly family protein"
                     /protein_id="HIR3339647.1"
                     /translation="MINALLYAGLAFYFWRTRWSAAGSAPAEGASFGVFEHYATLAPL
                     ALHTWLLARAMFAPDGLHLGVGNAVSAILWLTVLIYWLGNFHYRLDGLQSLVLPVAAA
                     ASLLPTVLPSLKPLPNTDLMAFKIHLLIAMAAYSLFTIASLHVLLMAMLERRLHDGAL
                     PRTLRGLPPLLAMETLLFRIIWAGFILLTLTIASGVAFSEELFGKAAKLNHKTVFGVL
                     SWIVFAALLGGRHVYGWRGRIAVRWTLAGFLMLVLAYIGSKFVLEVILGRG"
     gene            2222..3082
                     /locus_tag="AC158Q_07440"
     CDS             2222..3082
                     /locus_tag="AC158Q_07440"
                     /inference="COORDINATES: protein motif:HMM:NF013741.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIR3339648.1"
                     /translation="MEILILASLIFINALFAMSEVALLTARKPRLATLAKNGDMLAAA
                     AVKLAEEPTRFLSTIQIGITSIGLLSGIVGEAVLAAPLAVWFQSLGMEPKASGIVATA
                     IVVLFITYFSIVVGELVPKRLGQHNAEGIARLVSWPMRMLATASAPFVYLLSASTDAI
                     LKPLLKLLRIQHESGTQSLSVDEIRTIVLESSNFLPKKHVSILLNLFDLESITVNDVM
                     VPRNHLEAVDIDAPLEQIKHQIATAHHRRLLVYRGNLDNVTGTLRVRDVLNLLENDGL
                     TKERLQEIIH"
     gene            3092..3514
                     /locus_tag="AC158Q_07445"
     CDS             3092..3514
                     /locus_tag="AC158Q_07445"
                     /inference="COORDINATES: protein motif:HMM:NF015436.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HIR3339649.1"
                     /translation="MPAGTPLFSQMQNFQEQKDRMSLVVDEYGELQGLVTLEDILEEI
                     IGEFTTQSPLQTGGYTRQPDGSYLVEGATLLRELNRKLGFNFPLDGPKTLSGLILEHL
                     QTIPEPSTSVKIAGHPLEIVQTQGRVVKAVRVFPAPAA"
     gene            3637..4869
                     /locus_tag="AC158Q_07450"
     CDS             3637..4869
                     /locus_tag="AC158Q_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR3339650.1"
                     /translation="MATAAAAVRKASDVKEFNFTWIGKDKSNKVVRGEMKATGEAQVN
                     AALRRQGIRVEEIKQQKLGRGGSISDKDITLFVRQLATMMKSGVPLLQAFDIVGKGHT
                     NAAVARLLMDIKTEVETGSSLKQAFEKHPLYFDALFCNLVGAGEQAGILDSLLDRLAT
                     YKEKILAIKSKIKSALFYPVAIVIVAFIITAVIMLFVIPAFKQVFSSFGADLPTPTLI
                     VMGISEFFVEYWYAIFGVIGGAGYGFFWTWKRSEKMQIFMDKVMLRLPIFGDLVRKSC
                     IARWTRTLSTMFAAGVPLVEALDSVAGASGNHEYFVATKKIQGEVSTGSSLTSSMQGT
                     TVFPPMVIQMVQIGEEAGSLDAMLSKVADFFEAEVDDAVEALSSLMEPIIMVVLGTLI
                     GGLVVAMYLPIFKLGAAV"
     gene            4944..5804
                     /locus_tag="AC158Q_07455"
     CDS             4944..5804
                     /locus_tag="AC158Q_07455"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HIR3339651.1"
                     /translation="MSVFAQLQSSPATLITLAVLFGLMVGSFLNVVIHRLPRMVERQW
                     REEYAELAGTALPPAERFNLLVPRSACPKCGHKIAALENVPVASYIALGGKCSACRAP
                     ISPRYPVVEALAGLLAGYVAWRYGPGTAMLGALLFVWAMIALAFIDLDTFYLPDAITL
                     PLLWAGLLFNLGGAFTDIRSAVIGAAAGYLALWSVYWAYKLATGKEGMGYGDFKLLAA
                     IGAWLGWKMLPLVILLSSLLGAAVGLALIAFARHGRNVPIPFGPYLALAGLIALFWGE
                     PLTRQYLNLF"
     gene            5808..6413
                     /gene="coaE"
                     /locus_tag="AC158Q_07460"
     CDS             5808..6413
                     /gene="coaE"
                     /locus_tag="AC158Q_07460"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234741.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HIR3339652.1"
                     /translation="MPFCVGLTGGIGSGKSTAAEIFKELGAALVDTDEISRSLTAPGG
                     SALAEVRRQFGPEYIAADGSLDRDRMRARVFSDPDAKSRLEAILHPMIRAESRVRVAA
                     ARAPYVILVVPLLFETGACLDLVQRVLAVDCSEALQIERATRRSGISAEEVRAIMAAQ
                     LPRAARRARADDVIDNDSGMEALRGQVGKLHSRYLTLARSA"
     gene            6523..7278
                     /gene="zapD"
                     /locus_tag="AC158Q_07465"
     CDS             6523..7278
                     /gene="zapD"
                     /locus_tag="AC158Q_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006756831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HIR3339653.1"
                     /translation="MISYEYPLSERVRTLLRLEDLFERVDYFLHKNDPHEHHVALLSI
                     FEILEVSGRADLKSDLLQELERQKHTLESLRENPDVSEEALDNVLWQIDQASSRLFQA
                     SGKIGQELRENEWLMSIRQRTNIPGGVCEFDLPSYHYWLQQAPEQHRHDLQLWLAPFL
                     PMRDGIAIVLRLLRESGKTTAQVAYQGMYQQMMAGRMSQMLRIRLKREYHCVPEISAN
                     KYALNIRFTTQEGVQRPKAADADVEFDLTFCNL"
     gene            7324..7539
                     /locus_tag="AC158Q_07470"
     CDS             7324..7539
                     /locus_tag="AC158Q_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HIR3339654.1"
                     /translation="MNREPRSVACPKCGKSVLWTPENAWRPFCSERCKLVDLGAWAAE
                     QYRVPIVEDKDEPESGDSDTTPPASEM"
BASE COUNT         1295 a         2536 c         2417 g         1461 t
ORIGIN      
        1 tcatcttcat catcttctgc gtctgctcga actggttcaa cagccggttc acttcctgca
       61 ccgcgacgcc ggcgcccgcc gcgatgcggc gcttgcgtga ggccttgagg atctcgggct
      121 tcgctcgctc ctcgcgcgtc atcgcgttga tgatgccctc gatcctgcga atcgccttgt
      181 cgtccatccc cggcgcaccc tgcgcgagct gcccgagatt gccgggcagc ttgtcagcga
      241 gcgcggcaag cccgcccatc tttttcatct gcgcgatctg ctgcttgaaa tcctcgaggt
      301 cgaaatcctt cccggacttc acttttttcg cgagctttac ggcctcctcg tgatccacgc
      361 ccttctgcac atcctcgatc agcgagagca cgtcgcccat gccgaggatg cgcgaggcca
      421 tgcggtcggg gtggaacggt tccagcgcgg caagcttctc gccaacgccg gcgaacttga
      481 ccggctttcc ggtcacctcc cgcaccgaga gcgccgcgcc gccgcgggcg tccccgtcga
      541 gcttggtgag gatgatgccg gtcagcggca gcgcgtcggc gaaggccttc gccgtgttga
      601 ccgcgtcctg gccctgcatc gcgtccacca cgaacagcgt ctcgaccgga ttcagtgcgc
      661 ggtgcagttc gccgatctcg cgcatcatcg cctcgtcgat cgcgagccgg cccgcgctgt
      721 caacgatcag cacgtcggca taatgcttgc gggcgtaatc cagggcgcgc gtcgcgatgt
      781 cgagcggttt ctcgccgatg gcggaaggaa aaaacccggc ctcgacctgt aacgccagcg
      841 tcttcagctg ctcgattgcc gccgggcggt agacgtcgca acttgcgagc aggaccttct
      901 ttttcaactc ggtcttgagc cagcgcgcga gcttgcccgc agtcgtggtc ttgcccgccc
      961 cctgcagtcc cgcgagcagg attacggcgg gcggctgggc cgccaggttg agcgaatcgt
     1021 tcttctcgcc catcaatgcg acgagttcgc ggtaaaccac gcccaccgcc gcctggcccg
     1081 gcgtcaggct gccgacgact tcctggccca gcgccttctc gcgcacgcgc tcgatgaacg
     1141 cgcgcaccac cggcagcgcg acgtcggctt ccagcagcgc cacgcgcact tcgcgcagcg
     1201 cttcctggac gttggactcg gtgagccgcg cctcgccgcg cagcgtcttg atcacgcgcg
     1261 agaggcgccc ggtcaggttg tcgaacacgc ggggcgggtt agggtgacta gggggttggt
     1321 gtacactcgg aaagtgcctg aaattctacc ttatttgatc aatgccctgc tctacgcggg
     1381 gcttgcgttc tacttctggc gcacgcgctg gagcgcggcg ggcagcgcgc cggcggaggg
     1441 cgcttccttc ggcgtgttcg agcactacgc gacgctcgct ccgctcgcct tgcatacctg
     1501 gctgctcgcg cgcgccatgt tcgcgccgga cggcctgcac ctcggcgtcg gcaacgcagt
     1561 ctccgcgatc ctctggctca cggtgctgat ctattggctc ggcaatttcc actaccgcct
     1621 cgacggcctg caatcgctgg tgctgccggt cgcggcggcg gcgtccctgc tgccgacggt
     1681 gcttccgtcg ctgaagcccc tccctaacac cgatttgatg gcgttcaaga tccacctgct
     1741 gatcgcgatg gccgcctaca gcctgtttac catcgcttcg ctgcacgtgc tgctgatggc
     1801 catgctcgag cgccgcctgc acgacggcgc gctgccgcgg acgctgcgcg ggctgccgcc
     1861 gctgctcgcg atggaaacgc tgctgttccg catcatctgg gcgggtttca tcctgctgac
     1921 gctcaccatt gcaagcggcg tggcattttc agaggaattg ttcgggaaag cggcgaagct
     1981 gaaccacaag acggtattcg gcgtgctctc ctggatcgtc ttcgcggcgc tgcttggcgg
     2041 ccggcacgtc tacggctggc gcgggcgcat cgccgtgcgc tggacgctcg ccggctttct
     2101 catgctggtg ctcgcctaca tcggcagcaa gtttgtcctg gaagtgattc tcggccgcgg
     2161 ttgagcgcgt gggcgcccgg ccgccctgtg agatgtggca tccgcgcggc aaggtgaaat
     2221 catggaaatt ctgatactgg ccagtctgat attcatcaac gctcttttcg ccatgtccga
     2281 ggtggcgctg ctcaccgcgc gcaagccccg tctcgcgacg ctcgccaaga acggggacat
     2341 gctcgctgcg gcggccgtga aactcgccga ggagccgacg cgcttcctgt ccaccatcca
     2401 aatcgggatt acttccatcg gtctcttgag cggcatcgtc ggcgaggcgg tgctggccgc
     2461 gccgctcgcc gtctggttcc agtcgctggg catggagccc aaggcgagcg gtatcgtggc
     2521 caccgccatc gtcgtgctct tcatcacgta tttctcgatc gtcgtcggcg aactggtccc
     2581 caagcgcctg ggccagcaca acgcggaggg catcgcgcgg ctggtctcct ggccgatgcg
     2641 catgctcgcg acggcttccg cgccgttcgt gtacctgctg tcggcctcca ccgacgcgat
     2701 actcaagccg ctgctgaagc tgctgcgcat acagcacgag tcggggacgc aatcgctgtc
     2761 ggtcgacgag attcgcacga tcgtgctgga atcctcgaat ttcctgccga aaaaacacgt
     2821 ctcgatcctg ctgaacctgt tcgacctcga gtcgatcact gtgaacgacg tgatggtgcc
     2881 gcgcaaccat ctcgaggcgg tcgacatcga tgcgccgctc gagcagatca agcatcagat
     2941 cgccacggcg catcaccggc ggctgctggt ttaccgcggc aacttggaca acgtgaccgg
     3001 cacgctgcgc gtgcgcgacg tgctgaacct gctggaaaat gacgggctca ccaaggagcg
     3061 gctgcaggaa atcatccact agccgaactt cgtgccggcc ggcacgccgc tgttctccca
     3121 gatgcagaac ttccaggagc agaaggaccg catgagcctg gttgtggacg agtacggtga
     3181 gctgcagggg ctggtcaccc tggaggacat cctcgaggaa atcatcggcg aattcaccac
     3241 gcagtccccg ttgcagacgg gcggctacac ccggcagccc gacggcagct acctcgtgga
     3301 gggcgcgacg ctgctgaggg aactgaaccg caagctcggc ttcaacttcc cgctcgacgg
     3361 gccgaagacg ctgagcgggc tgatcctcga gcacctgcaa accatacccg agcccagcac
     3421 cagcgtgaaa atcgccggcc acccgctgga aatcgttcag acccagggac gggtggtaaa
     3481 agcggtgcgg gtgtttccgg cgccagccgc gtgaatatgc cgccgcccgg cccgcggtgt
     3541 ggcaaactag gctttacaaa ggcaggcaag atgggcatta tcctgttaac aagcttgcct
     3601 aagctgttgg ttcattaggg taattggggg tcaatcatgg caactgcggc cgcggcggtc
     3661 agaaaagcat ctgacgtcaa ggaattcaat ttcacctgga tcggcaagga caagtccaac
     3721 aaggttgtcc gcggcgagat gaaggcgacg ggcgaagcgc aggtcaacgc cgcgctgcgc
     3781 cgccagggca tccgggtcga ggagatcaag cagcagaaac tcggccgggg cgggtcgatc
     3841 tccgacaagg acatcacgct cttcgtccgc cagctcgcga cgatgatgaa atcgggcgtg
     3901 ccgctgttgc aggctttcga catcgtcggc aagggccaca ccaacgcggc ggtcgccagg
     3961 ctgctgatgg acataaagac cgaggtcgag accggctcca gcctgaagca ggcgttcgag
     4021 aagcacccgc tctatttcga cgccctgttc tgcaacctcg tgggcgcggg ggagcaagcc
     4081 ggtatcctgg actccctgct cgaccggctc gcgacctaca aggaaaaaat cctcgccatc
     4141 aagagcaaga tcaagtctgc cctgttctac ccggtcgcga tcgtcatcgt ggcattcatc
     4201 atcaccgcgg tgatcatgct gttcgtgatc ccggcgttca aacaggtgtt ctcgagcttc
     4261 ggcgccgacc tgccgacccc gacgctgatc gtcatgggga tctcggagtt cttcgtcgaa
     4321 tactggtacg ccatattcgg cgtcatcggc ggcgcgggct acggcttctt ctggacctgg
     4381 aagcgttccg aaaaaatgca gattttcatg gacaaggtga tgctccggct gccgatattc
     4441 ggcgacctgg tacgcaagtc gtgcatcgcg cgctggacgc ggacgctgtc gacgatgttc
     4501 gcggcaggcg tgccgctggt cgaggcgctc gactcggtgg ccggcgcgtc cggcaatcac
     4561 gaatatttcg ttgcaaccaa gaagatccag ggcgaggtct cgaccggctc gagcctcacc
     4621 tcctcgatgc agggcaccac ggtttttccc cccatggtga tccagatggt gcagatcggc
     4681 gaggaggcgg gctcgctcga tgccatgctg tccaaggttg ccgacttttt cgaggccgag
     4741 gtggacgacg ccgtcgaggc gctgtcgagc ctgatggaac cgatcatcat ggtggtgctg
     4801 ggcacgctga tcggcggcct cgtggtcgcg atgtatctgc ccatcttcaa gctgggcgcc
     4861 gcggtataaa ccgcaggatt ggggatcgag ttgtcagggt tgaggccggg ccgcagccct
     4921 cgatcctcga tccccggtcc ttgatgagcg tcttcgcgca gctccaaagt tcccccgcga
     4981 ccctcatcac cctcgcggtg ctgttcggcc tgatggtcgg cagcttcctc aacgtcgtca
     5041 ttcaccgcct gcccaggatg gtcgagcgcc agtggcgtga agagtacgcc gagctggccg
     5101 ggaccgcgct gccacccgcg gaacgattca acctgctggt gccgcgctcc gcctgcccga
     5161 agtgcggtca caaaatcgcg gccctcgaaa acgtcccggt cgcgagctac attgccctcg
     5221 gcggaaaatg ctcggcgtgc cgggcgccga tctcgccgcg ctatccggtg gtggaggccc
     5281 ttgccgggct gctggccggc tacgtggcgt ggcgttacgg gcccggcacc gcgatgctgg
     5341 gggcgctgct tttcgtctgg gcgatgattg cgctcgcctt catcgacctc gacacctttt
     5401 atctcccgga cgccatcacg ctgccgctgc tctgggccgg attgctgttc aacctaggtg
     5461 gcgcctttac cgatatccgc tcggcggtca tcggcgccgc ggccggctac ctcgcgctat
     5521 ggtcggtgta ctgggcttac aagctcgcta ccggcaagga gggcatgggc tatggcgatt
     5581 tcaagctgct cgccgccatt ggcgcctggc tcggctggaa gatgctgccg ctcgtgatcc
     5641 tgctctcctc cctgctcggt gccgcggtgg gactggcgtt gatagcgttc gcgcgccacg
     5701 gccgcaacgt cccgattcca ttcggcccct acctcgccct cgccggattg atcgcgctgt
     5761 tctggggcga gccgctgacg cggcagtact tgaatctttt ctgagttatg ccgttttgcg
     5821 tcggcctcac cggtggcatc ggatcaggca agtcgaccgc ggccgaaata ttcaaggaac
     5881 tcggcgcggc gctggtggac accgacgaga tctcgcgcag tctcaccgca cccggtggca
     5941 gcgcattagc cgaagtccgc cggcaattcg gcccggagta tatcgcggcg gacggcagcc
     6001 tcgaccggga ccgcatgcgc gcgcgggtgt tcagcgaccc ggacgcgaaa tccaggctcg
     6061 aagcaatcct gcacccgatg atccgcgcgg agtcgcgcgt gcgggttgcc gcggcgcgcg
     6121 cgccctacgt catcctggtc gtgccgctcc tgtttgaaac cggcgcgtgt ctggatctcg
     6181 tgcagcgcgt gctggcggtg gactgcagcg aggcgctcca gatcgagcgc gccacgcgcc
     6241 gcagcgggat ttccgccgag gaagtgcgcg cgatcatggc ggcgcagctc ccgcgcgcgg
     6301 cgcggcgcgc gcgcgccgat gacgtgatcg acaatgacag cggcatggag gcgctgcgcg
     6361 gccaggtcgg gaaactccat tcccgctacc tcacgctggc tcgctcggca tgagcacccg
     6421 ggcgcgcgct gtgcttcaac aatcctcaat aaacccttta tttttttcgg gttttgatga
     6481 atacttagtt gcaaatccgg ccctcgaacg gatcatccca acgtgatcag ctacgagtat
     6541 ccactgagcg agcgcgtgcg cacgttgctg cggctcgagg acctgttcga gcgcgtcgat
     6601 tatttcctgc acaagaatga cccgcacgag caccacgtcg cgctgttgtc gatcttcgag
     6661 attctcgagg tgtcggggcg cgcggacctg aaatccgatc tcctgcagga gctggagcgg
     6721 cagaagcaca ccctcgaatc cctgcgcgag aaccccgacg tgtcggagga ggcgctcgac
     6781 aacgtcttgt ggcagatcga ccaggcctcc tcccgcctgt tccaggcctc gggcaagatc
     6841 gggcaggaac tgcgcgagaa cgagtggctg atgagcatac gccagcgcac caacattccg
     6901 ggaggcgtct gcgagttcga tctgccgtcg taccactact ggctgcagca ggccccggaa
     6961 cagcaccgcc acgatctcca gttgtggctg gcgcccttcc tgccgatgcg tgacgggatc
     7021 gcgatcgtgc tgaggctgct gcgtgaatca ggcaagacca ccgcgcaggt cgcctaccag
     7081 ggcatgtacc agcagatgat ggccgggcgc atgtcgcaga tgctgcgcat ccggctgaag
     7141 cgcgagtacc actgcgtgcc ggagatcagc gccaacaagt acgcgctcaa tatccgcttc
     7201 accacccagg aaggcgttca acggccgaaa gcagccgacg ccgacgtcga gttcgatctc
     7261 accttctgca acctgtagga attcaccgga ccggagccgg acccatttct cacccggcat
     7321 ggcatgaatc gagaaccacg ttccgtcgcc tgcccgaaat gcggcaagag cgtgttgtgg
     7381 acgccggaga acgcctggcg gccattctgc tccgagcgct gcaagctggt cgatttgggc
     7441 gcgtgggccg ccgagcagta ccgtgttccc atcgtagaag ataaagacga accggagtcc
     7501 ggcgattccg acacgacgcc acctgcatct gaaatgtaac tccctgccat agggttgccg
     7561 tcgctgcact cgcgcaagaa tcagctccag gccccacgca tcatgctgat gccgtgtgcg
     7621 ccggattccc aggctcgctc caggtctgcg gccttgagcc cgcccagcgc atagaccggc
     7681 acccggctgt ccttcaacag cccggcgaa
//
LOCUS       DBNGSG010000286         4794 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2975261, whole genome shotgun sequence.
ACCESSION   DBNGSG010000286 DBNGSG010000000
VERSION     DBNGSG010000286.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4794)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2975261"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="AC158Q_07475"
     CDS             complement(<1..405)
                     /locus_tag="AC158Q_07475"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3339655.1"
                     /translation="MDQTVEAPASLLPAQRPSPARIKKLGHVVIQVRNRERSIRFYTE
                     VLNFRVSDNATAGGVFLTAIGDHHTIGLFDSDGENAEIPSKGAVRLHHFAMQVDSLEE
                     LFEFRAYLKERGIPIVWEGRRAMGGHTSVEFLD"
     gene            629..1612
                     /locus_tag="AC158Q_07480"
     CDS             629..1612
                     /locus_tag="AC158Q_07480"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3339656.1"
                     /translation="MKNGGLIIAFIALSLAAGGGYAQQATSTGSGQGYPNKPIRLIVP
                     FPPGGQTDVVARTISQKLSEAFGQSVVVDNRPGAAGSIGAEIAVNAPADGYTMIQIST
                     SYAGNVALRKLPYDPINDVVPVVMIGEIANLVTVNPSGPYKNIKELIAYAKANPGKIN
                     YASGGTGSGNHLATEEFAQMAGIRMTHVPYKGSTAGVNDLMGGRIQLIFSGITGMIPH
                     HKSKRVLGIAVTSAKRNQAVPELPTVGETLPGYASVSWSAILAPKGTPGQITARWNAE
                     LNRILQMPDVKARMDSIGLDVVGGTQAHLRKVLTQDIATWKRVVKTADIKL"
     gene            complement(1641..2498)
                     /locus_tag="AC158Q_07485"
     CDS             complement(1641..2498)
                     /locus_tag="AC158Q_07485"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR3339657.1"
                     /translation="MNRIIVACSAAFALLSAAPSAAQLDLGFPAEPEREVRLPGRGAF
                     TGARLYAPEGAGPFPAVVLSHTSGALREHVFEWAQRFHAAGYAALIVDHLGPRNKRTN
                     TDFSVSVTEYAQDDVAAMRHLRTLPFVDGKRIVQMGFSYGGMAGLREASKDFRDRNLG
                     GERFAAIVSVYPWCHHQGPGRERRDHHWNFFDDTDIPLLVLVGADDDEADPRSCITKA
                     KANAGRGLPVELAVFPNTTHAYDHSWMGDKPVMARLGNRMVTYRFNRESVEASWKLTL
                     DFLARHLKQ"
     gene            complement(2646..2981)
                     /locus_tag="AC158Q_07490"
     CDS             complement(2646..2981)
                     /locus_tag="AC158Q_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339658.1"
                     /translation="MESFIDHWAKQRAASEGDSLYARLYVSVHIGERVAPDTTRYNWA
                     RMKKSLEDLIARYPDPVHKNRYASYACVARDKNVFGEAMRRLPPNEIDRNAWLHGHSY
                     EACLRWAGI"
     gene            complement(3020..3880)
                     /locus_tag="AC158Q_07495"
     CDS             complement(3020..3880)
                     /locus_tag="AC158Q_07495"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR3339659.1"
                     /translation="MLKIIAAISGLAFLASCATTPTLSLSPQARSELAPTGKVRVGLN
                     YGNPLFARRGADGEGSGIAVDLARELGRRMGVAVELVGYDSGGQLTAGLNAGGWDIAF
                     LAYEQTREKEITFASHFAEIEGSYLVPAGSPFRNAAEVDREGVRIAVSQGGGNALFLA
                     RALKHAKLVPMKGSAAAFKAFVADKLDAYAGLKPTLINVSAKLPGSRVLDGSYTVIPY
                     SVGTLKGRDAGARYLRDFIEEAKASGFVGRSIEKNGIRGVSVARPAPVAQPGSEMYEI
                     LHRITNTGTD"
     gene            complement(4051..>4794)
                     /locus_tag="AC158Q_07500"
     CDS             complement(4051..>4794)
                     /locus_tag="AC158Q_07500"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3339660.1"
                     /translation="YERGDVRVHYEEAGSGFPLMLIPGGGLNSSFASWQAAPFKPMEI
                     FKDDFRCICAELRNANPGQSSGPLEVDRPWDAYTDDQLGLMDHLGIREFLVMGFCIGG
                     PMIHNLIRRAPERVVAAAMIQPSGYRPEIPDNFRQNNMKGWGPALCARRPDITMDRVH
                     DFLTNMYTNRADFVFTVTRDFVRSMKTPLLIAPDDVPSHPYKVAMEVASLAPNAEVTI
                     YPWKESKETIDEVVAHARRFLKMHEPVRV"
BASE COUNT          867 a         1557 c         1496 g          874 t
ORIGIN      
        1 atcgaggaac tcgacgctgg tgtgaccacc catggcgcgc cggccttccc acacgatcgg
       61 gatgccgcgc tccttgagat aggcgcggaa ttcgaacagc tcctccaggg aatccacctg
      121 catggcgaag tgatgcagcc ggaccgctcc cttggaggga atctccgcgt tttccccgtc
      181 agagtcgaaa aggccgatgg tgtgatggtc tcctatggcg gtcaggaaca ccccgccggc
      241 cgtagcgtta tccgagacgc gaaagttcaa tacctccgtg tagaagcgta tcgagcgttc
      301 gcggttgcgc acctggatga ccacgtgacc cagcttcttg atgcgggccg ggctgggacg
      361 ctgggcgggc agcagggaag ccggggcctc gactgtttga tccatacggt tatctccttg
      421 tcttgcgcgg gcgtgccttc agttcgctgt gagcgtagcc tcgaccgcgg ccgaaggcaa
      481 gtcgccttgc acctgcggcg cgagcccccc ggctgcaagg ccgcgcggac agttgccatg
      541 cttccgccat tggcatagat tgccgggaaa tcctgcgctg aacggcaggc gcgtcaggca
      601 gggacaatag ttccgacagg aggagtccat gaaaaacggc gggctgataa tcgcatttat
      661 tgcactttca ctggcagcgg gcggcggtta cgcccagcag gccacttcga caggctcagg
      721 acagggctac ccgaacaagc cgatccggct gatcgtgccc ttcccgcccg gcggccagac
      781 cgatgtcgtg gcacgcacca tatcgcagaa actctccgag gctttcggtc agtcggtggt
      841 ggtggacaac cggcccggcg cggcaggaag catcggcgcc gaaatcgcgg tgaatgcgcc
      901 cgccgacggc tacaccatga tccagatatc aacgagctac gccggcaacg tggcgctgcg
      961 caagctgcct tatgacccga tcaatgacgt cgtgcccgtc gtgatgatcg gcgagatcgc
     1021 caatctggtg acggtcaatc cgtcgggccc ctacaaaaac atcaaggagt tgatcgccta
     1081 cgccaaggcg aatcccggca agatcaacta tgcctcggga ggcaccggaa gcggcaacca
     1141 ccttgccacc gaggagttcg cccagatggc cggcatccgg atgacgcacg tgccgtacaa
     1201 gggttccacc gccggcgtaa acgacctgat gggcggccgc atccagctca ttttcagcgg
     1261 cattacgggg atgatcccgc accacaagtc gaagcgcgtg ctcggcatcg cggtgaccag
     1321 cgcgaagcgc aaccaggccg tgccggaact cccgaccgtc ggcgaaacct tgcccggcta
     1381 tgcatccgtg agctggtcgg ccattctggc gcccaagggc acgcccgggc aaattaccgc
     1441 gcgctggaac gcggagctca atcgcatcct gcaaatgccc gacgtgaagg cgcgcatgga
     1501 ctcgatcggc ctggatgtgg tcggcggcac gcaggcgcat ctgcgcaagg tcctcacgca
     1561 ggacatcgcg acgtggaaga gagtggtcaa gaccgccgat atcaagctgt aagcggtctc
     1621 taaaacaagt caaaggccat ttactgcttc aggtgccgcg ccaggaaatc gagcgtcagc
     1681 ttccagctcg cctcgacgga ttcccggttg aagcggtagg taaccatccg gttgcccagc
     1741 cgtgccatga cgggcttgtc gcccatccat gagtggtcgt acgcgtgcgt ggtgttcggg
     1801 aacacggcga gctccaccgg caacccccgg cccgcgttcg ccttggcctt ggtaatgcac
     1861 gagcgcgggt cggcctcgtc atcgtcggcg ccgaccagga ccagcagcgg aatatcggtg
     1921 tcgtcgaaaa aattccagtg atgatcccgg cgctcccggc cggggccctg gtggtggcac
     1981 cacgggtaga cgctgacgat cgccgcgaag cgctccccgc ccaggttcct gtcgcggaag
     2041 tccttgctcg cctcgcgcag ccccgccatc ccgccgtagg agaatcccat ctggacgatg
     2101 cgcttgccgt ccacgaacgg cagggtgcgc aggtgccgca tggcggccac gtcgtcctgg
     2161 gcatactcgg ttacgctgac gctgaaatcg gtgttcgtcc gcttgttgcg cgggccgaga
     2221 tgatccacga tcagggccgc gtagccggcg gcatgaaacc gctgcgccca ttcgaagacg
     2281 tgctcgcgca gcgcgccgct ggtgtgcgag agcaccaccg ccggaaacgg gccggcgcct
     2341 tccggagcgt aaagcctcgc cccggtgaac gcgccgcggc ccggcagcct gacctcgcgt
     2401 tcgggctcgg ccgggaatcc aagatccagt tgcgcggcgc tcggtgccgc gctcaaaagt
     2461 gcaaaagctg ccgagcatgc aacgatgatc cggttcatcg gtcgccctcc gcatttagcg
     2521 ataataatat cccttcacgc agccgggcga ggccacatac gatgcgcgat ccgtgcggtt
     2581 cgcgctcgat cagcaggttt aacccgcgcc tagaaggtca gttcagtcca acccggagtt
     2641 ggagactaga ttccagccca ccgcaggcag gcttcgtatg agtggccgtg cagccatgca
     2701 ttccgatcga tctcgttcgg cggaagccgg cgcatcgcct ctccgaacac gttcttgtcc
     2761 ctcgccacgc acgcgtagct cgcatatcga ttcttgtgaa ccgggtcggg atatcgggca
     2821 atcaggtcct cgagactctt cttcatccgg gcccagttgt agcgggtcgt atccggcgcg
     2881 actctctcgc caatgtgcac gctcacgtag agcctcgcgt acagcgaatc gccttcactg
     2941 gcggcccgtt gtttcgccca atgatcaatg aaggactcca ccgcctccca gttgccgccc
     3001 cacttgggtg tcagtcgcgt cagtccgttc ccgtatttgt tattcgatgc aatatttcgt
     3061 acatctcgct gcccggctga gcgaccggcg caggccgcgc cactgaaacg ccgcgtatgc
     3121 cgttcttctc gatcgagcgg ccgacgaatc ccgaagcctt tgcctcctcg atgaaatcgc
     3181 gcagataacg ggcgcccgcg tcgcggccct tcagagtgcc gaccgaatac gggatcaccg
     3241 tgtagctccc gtcgagcacg cgcgagccgg gcagcttcgc ggatacgttg atcagtgtcg
     3301 gcttcaggcc ggcgtaggcg tcgagcttgt ccgcgacgaa cgccttgaat gcagcggcgc
     3361 tgcccttcat gggcaccagt ttcgcgtgct tgagcgcgcg ggcgaggaac agcgcgttgc
     3421 cgcctccctg cgaaacggcg atgcgcacgc cctcgcggtc gacttccgcg gcgttgcgaa
     3481 acggggaccc cgcgggcacc aggtagcttc cttcgatctc cgcgaagtgc gaggcaaagg
     3541 taatctcttt ttcacgcgtc tgttcgtacg cgaggaacgc gatgtcccag cccccggcat
     3601 tcaatcccgc ggtcaactgg ccgcccgaat cgtagccgac cagctcgacc gcgacgccca
     3661 tccgccggcc gagctcgcgg gcaagatcga cggctatgcc gctcccctcg ccatccgccc
     3721 ctctcctggc gaacaacgga ttgccgtaat tgagccccac gcgcaccttg cccgtcggcg
     3781 cgagttccga acgcgcctgc ggcgagagcg agagcgtggg cgtggtggcg caggacgcca
     3841 ggaaagccag accggaaatc gccgcaatta ttttgagcat gattcccccc tgttcgccct
     3901 gcgtactgag caacagaccg gggtcggagc cccagttcct tacttcgctg caatacgttc
     3961 caggaaatag ggctccgtcc ccttgttatc ccaggaattt ctgtgtggtt ccgtgtggtt
     4021 ccgtggctaa agcggttttt gagatgactt ctagaccctg acgggttcgt gcatcttgag
     4081 gaagcgccgc gcatgcgcca cgacttcgtc gatggtctcc ttggattctt tccagggata
     4141 gatcgtcacc tcggcgttcg gggcgaggct cgccacctcc atcgcgacct tgtacgggtg
     4201 cgagggcacg tcgtcgggcg cgatcagcag cggcgtcttc atggagcgca cgaaatcgcg
     4261 cgtcacggtg aaaacgaagt cggcgcggtt ggtgtacatg ttggtcagga agtcgtggac
     4321 cctgtccatc gtgatgtcgg gccgcctcgc gcacagcgcc ggcccccagc ctttcatgtt
     4381 gttctggcgg aagttgtccg ggatctcggg ccggtagccg ctgggctgta tcatcgcggc
     4441 cgcgaccacg cgctccggcg cccgccgaat caggttgtgg atcatcgggc cgccgatgca
     4501 aaagcccatg accaggaatt cccggatgcc gagatgatcc atcagcccga gctggtcgtc
     4561 ggtgtaggca tcccaggggc ggtccacctc cagcgggccg ctggactggc cgggattggc
     4621 gttgcgcaac tcagcgcaga tacagcggaa gtcgtccttg aagatttcca tcggcttgaa
     4681 cggcgccgcc tgccaggacg cgaaggacga attgagtccg ccgccgggga tgagcatcag
     4741 cgggaagccg gagccagcct cttcgtaatg gacccggacg tcgcctctct cgta
//
LOCUS       DBNGSG010000287         5216 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3485949, whole genome shotgun sequence.
ACCESSION   DBNGSG010000287 DBNGSG010000000
VERSION     DBNGSG010000287.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5216)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5216)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5216
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3485949"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="AC158Q_07505"
     CDS             <1..509
                     /locus_tag="AC158Q_07505"
                     /inference="COORDINATES: protein motif:HMM:NF024901.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HIR3339661.1"
                     /translation="TVISFHEADNPAGMNSRRCPVGNVMWKACIAALLCAAAGAPAQT
                     VHKQVDDTGHVTYSDRPDMTPPTLPLPRMTAVSALDVARALAANIPISSRGAARVDAS
                     EAARRLRRAQRERELGAEPLPGEQAPGADARAVNDRYRQRQEKLRHAVEQAQRRSSQL
                     RAPLRTPR"
     gene            1159..1284
                     /locus_tag="AC158Q_07510"
     CDS             1159..1284
                     /locus_tag="AC158Q_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339662.1"
                     /translation="MIPLVTYDLGYVKLNAVYFPKFGHYNEVDAVGFYISIPFSK"
     gene            complement(1990..3195)
                     /gene="gspF"
                     /locus_tag="AC158Q_07515"
     CDS             complement(1990..3195)
                     /gene="gspF"
                     /locus_tag="AC158Q_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HIR3339663.1"
                     /translation="MEAFQYQALDADGRTVSGVVQADTARQARTQVRAQGLLPSVIGK
                     VRARERARQAWARGISASELSLLTRQMALLLDSGLTMEQALNALIEEADTPIAREVLT
                     GVKTEVTSGLSFAAALGAYGRSFPDFYRALVHGGEESGALPTVLQHLADYLDARQALK
                     QKTGLALLYPALVTLVAVMIVTGLLVYVVPQVVEVFQQSRQSLPWLTRALIALSDFLR
                     GAWPYLAAAVIGGWAAAHMTLRRDAARRRWHALLLRLPGLGPLIRGVNTSRFASTLAI
                     LVGGGVPLLSALASGAQVMTNQVLKQAVERAIERVREGASLARALGETRAFPPLLVHL
                     VASGEASGKLEQMLERAAQLETRALERRLAVFLTLLEPVMILVMGAIVLLIVLAILLP
                     IIEINQLVR"
     gene            complement(3215..4684)
                     /gene="gspE"
                     /locus_tag="AC158Q_07520"
     CDS             complement(3215..4684)
                     /gene="gspE"
                     /locus_tag="AC158Q_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HIR3339664.1"
                     /translation="MKPAAIPYAFAQANGIIVTALEKDSAEVALRDGAPPRALAEVRR
                     ALGLPLRARRVGADEFDELVSALYNGADAGAAVLAGDLAQDIDLSRLLQEIPRVEDLL
                     DSQDDAPVIRLINAMLTQALRDGASDIHIEPFEARSVVRLRIDGTLRDLIEPARALHA
                     AIVSRVKIMAQLDIAEKRLPQDGRITLRVAGKPIDVRVSTIPTGHGERVVLRLLDKQA
                     GRLDLARLGMDDAELAHMDRLIRDPHGIVLVTGPTGSGKTTTLYAALSRLDATALNIM
                     TVEDPIEYDLDGISQTPINTRIEMSFARALRTILRQDPDVVMIGEIRDLETAQIAVQA
                     SLTGHLVFATLHTNDAVSAVTRLVDMGVEPFLLASSLIGIGAQRLVRRLCLECRRPFA
                     ADAAQLRALGLAPAGGTLYTAQGCPACNRTGYRGRTGIYELLTVDDELRRLIHDRASE
                     QALRASVVARGMRSLRDDGMRWAAQGVISLEEVVRVTRE"
     gene            complement(4702..>5216)
                     /locus_tag="AC158Q_07525"
     CDS             complement(4702..>5216)
                     /locus_tag="AC158Q_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HIR3339665.1"
                     /translation="GGAVTPTPVQTIERRDVGLTLRIKPQISEGGTVRLQIYQEVSSV
                     VDTIVTPSAGPTTNKRAVETTVLVDDGQIVVIGGLIQDSVKDGVEKVPLLGDIPLLGA
                     LFQYKTRSRSKTNLMVFLRPVVVRDGQGSAVLSGERYDYIIGEQRKARPAPSLGLPDV
                     EAPELPPMNP"
BASE COUNT          843 a         1772 c         1743 g          858 t
ORIGIN      
        1 gaacggtgat ttcattccac gaggccgaca atccggcggg tatgaactct cgccgctgcc
       61 ctgtggggaa tgtcatgtgg aaagcctgca ttgcggcgtt gctttgcgcc gcggcgggcg
      121 cgccggcgca aacggtccac aagcaggtcg atgacacggg gcatgttacg tactccgatc
      181 ggccggacat gacgccgcca acgttaccgt tacctcgcat gacggcagtc tcggcgctgg
      241 acgtggcgcg cgcgctggct gccaacatcc ccatttcctc gcgcggcgcc gccagggtgg
      301 acgcgagcga agcggcgcgc aggctgcgcc gggcgcaacg ggagcgcgag ctgggcgcgg
      361 agccgctgcc cggcgagcag gccccgggcg ccgatgcgcg cgcggtgaac gatcgctacc
      421 ggcagcgcca ggaaaaactg cggcacgccg tcgagcaggc acagcgccgg tcgagccagc
      481 tccgcgcacc cctgagaact ccgcgctgaa tcgactgact ggggctattc gcgcatcctg
      541 tcgggagatg cgggtgcgag gacccgcatg gcggcgagaa ttttttccgg cttgcgtacg
      601 ctggcgtacg gacaaaatcc cacgttgtgg ccgccatcgc gaaggaacat actcgcggcc
      661 gcctcgtaag cacagcgcct gaccttcgaa ggttgccccg tcagccgcgc tggtgcgaca
      721 ggcaaggggc agatgaacta tctcgattcc gggttgctgg ctttcgcggt tgccctgggg
      781 ttgtgcggtt gcggcgccac acttgccgac gaaagcgttg aaacttcgca tgacgtcaga
      841 ccggccgata actgactcaa tctcgaagcg ctcgaactca acgtctacgg gctctcctac
      901 catcccgacc gcaagaccgc caagcgcctg ggactggata atgaagtcaa tcccggactc
      961 ggtcttcact ataaactggc cgatgatgcg cgcggcgtca cctttgccga gatcggcgcg
     1021 taccgtgatt cggggcacaa ctgggcgaag ttcgcggcgc tgggctacca gttcaaactc
     1081 ggcgagcgct ggaagctagg cggcgccctt gccctcttca acagccggac ctacaaccgc
     1141 ggtgcggcct ttatcggcat gattccgctg gttacctacg atctgggcta cgtcaagctg
     1201 aacgcggtgt actttccgaa gttcgggcat tacaacgagg tcgatgccgt cggcttttac
     1261 atcagcatac cgttcagtaa gtgaaccctc ccggcgcgcg ccggacctga ttatctcagg
     1321 ggcatttcac cgggcaggcc cggcaatccg gcgggaatcc gttgtgaagc agtgcttacg
     1381 cggctttggc tgcccggtac gcggcaatgc ctgccgtgcc ggcaccgatt attgcaacat
     1441 caacttccag cgccttcatg ccttatcctc gttttgccga cggacgccgc ccggacgagc
     1501 aaggccccgt ctaccgcaac gggggcccca caagttcatc gtcgaacccg ccaataccag
     1561 gccaggtttc gctccggacc cgcgttagcg agccggcacg atcacgacgg tgccgccggt
     1621 atctccagtt gagccagtcc ggccggttga gccggtcctg ccggttgcgc cggtatctcc
     1681 ggttgcgccg gtgtatccgg tcgagccggt cgagccggtt ctaccggttg cgccggtgta
     1741 tccggtcgag ccagtcgagc cggtagcgcc ggtatctcca gtggcacctt gcggaccggc
     1801 cggccccgcg ggcaacgtca agcaaccacc cagcgcgagt gcggctatcc ctgcgacaaa
     1861 cagtttcgat tgcttcatgg tgatttcctt tgttcggcta acacgaacta cgcacgttat
     1921 tgtgagcagt cacccgcacg aggctacgcg aaccgccctg ccgcctcggt gcgctggcgc
     1981 gcatagtgtt cagcgcacca actggttgat ctcgatgatg ggaagcagga tagcgagcac
     2041 gatcagcagc acgattgccc ccatcaccag gatcatcacc ggctcgagca gcgtcaggaa
     2101 caccgccagg cgccgctcga gcgcccgggt ttccagctgc gcagcgcgct cgagcatctg
     2161 ctcgagcttc ccgctcgctt ccccgctcgc caccaggtgc accagcagcg gaggaaaagc
     2221 gcgcgtctcg cccagggccc gcgccaggct cgcgccttcg cgcacccgct cgatggcccg
     2281 ttcgaccgcc tgcttcaaca cctggttggt catcacctgc gcgccggaag cgagcgcgct
     2341 gagcaacggc acgccaccgc cgacaaggat ggcgagcgtg ctggcgaagc gcgaggtgtt
     2401 gacgccgcga atcagcggtc ccagcccggg caggcgcagc aggagagcgt gccagcggcg
     2461 ccttgccgca tcgcggcgca gcgtcatatg cgcggctgcc catccgccga tcacggcggc
     2521 cgcgagatac ggccaggccc cgcgcagaaa atcactcaag gcaatcagcg cgcgagtgag
     2581 ccacggcagg ctctgccgcg actgctggaa cacttccacc acctgcggca ccacgtagac
     2641 caggaggccg gtgacgatca tgacggccac gagcgtgacg agcgcggggt agagcagggc
     2701 gaggccggtt ttctgcttca gcgcctggcg ggcgtcgaga taatcggcaa gatgctgcag
     2761 cacggtcggc agggcgcccg actcctcgcc gccatgcacc agcgcccggt agaaatccgg
     2821 gaagctcctg ccgtaggcgc ccagcgccgc ggcaaacgac aggcccgagg tgacttcggt
     2881 cttcacgccc gtcagcacct cccgcgcgat cggcgtgtcg gcctcctcga tcagggcgtt
     2941 gagcgcctgc tccatcgtca ggccggagtc gagcagcagc gccatttgcc gggtgagcag
     3001 gctcagctcg gacgccgaga tgccgcgcgc ccaggcctgc cgcgcgcgct cgcgcgcccg
     3061 gaccttgccg atcaccgagg gcagcagacc ctgtgcgcgc acctgcgtgc gcgcctggcg
     3121 cgcggtgtcg gcctgcacca cgcccgatac cgtgcggccg tcggcgtcca gcgcctggta
     3181 ttggaatgct tccatggcgg ggccggggtt ttccctattc gcgcgtgacg cgcacgacct
     3241 cttcgaggct gatcacgcct tgcgcggccc agcgcatgcc gtcatcgcgc aacgagcgca
     3301 tgccgcgggc aacgacactc gcccgcagcg cctgttcgga ggcacgatcg tggatcagcc
     3361 ggcgcagctc gtcgtcgacc gtcagcaatt cgtagatgcc ggtgcgcccc cggtatccgg
     3421 tgcggttgca ggcggggcag ccttgcgccg tataaagtgt tccgcccgcc ggcgcgagcc
     3481 ccagcgcgcg gagttgcgcc gcatcggcgg caaagggcct gcggcattcc aggcacagcc
     3541 ggcgcaccag gcgctgggcg ccgatcccga tcaggctgga ggccagcaga aacggctcca
     3601 cccccatgtc gacgagccgg gtgacggcgc tgaccgcatc gttggtgtga agggtggcga
     3661 acaccaggtg gcccgtgagg ctcgcctgga cggcgatctg cgcggtctcc aggtcgcgaa
     3721 tctcgccgat catgaccacg tcgggatcct ggcgcaggat ggtgcgcagc gcgcgcgcaa
     3781 aactcatctc gatccgggtg ttgatcgggg tctggctgat gccgtcgagg tcgtactcga
     3841 tcgggtcctc gacggtcatg atgttgaggg ctgtcgcgtc caggcgcgac agcgcggcgt
     3901 acagcgtcgt cgtcttgccc gagccggtcg gcccggtgac cagcacgatg ccgtgagggt
     3961 cccggatgag ccggtccatg tgcgcgagtt cggcgtcgtc catacccagc cgcgcaaggt
     4021 cgaggcgtcc ggcctgcttg tcgagcaggc gcagcaccac gcgctcgccg tgcccggtgg
     4081 gaatcgtcga cacccgcaca tcgatcggct tgcccgccac ccgcagcgtg atgcgcccgt
     4141 cctgcggcag gcgtttctcg gcgatgtcga gttgcgccat gatcttcacg cgcgagacga
     4201 tcgccgcatg cagcgcgcgc gccggctcga tcaggtcgcg cagcgtgccg tcgatgcgca
     4261 gccgcaccac cgagcgcgcc tcgaacggct cgatgtggat gtcggaggcg ccatcgcgca
     4321 gcgcctgcgt gagcatcgcg ttgatcaggc ggatgaccgg cgcatcgtcc tggctgtcga
     4381 ggaggtcctc tacccggggg atttcctgca gcaaccggga gagatcgata tcctgggcga
     4441 gatcgccggc gagcaccgcc gcgcccgcgt ccgcgccgtt gtagagcgcg gagacgagct
     4501 cgtcgaattc atccgcgccg acgcggcgcg cgcgcaacgg caggccgagc gcgcgccgga
     4561 cttcggcgag cgcgcgcggc ggggccccgt cgcggagcgc cacctccgcc gaatctttct
     4621 ccagcgcggt gacgatgatg ccgttggctt gcgcgaaagc gtagggaatg gcggcgggtt
     4681 tcatcagggc aggtacctga tttatggatt catgggcggc agctcgggcg cttccacgtc
     4741 gggcagcccc aggctgggcg cgggcctggc cttgcgctgc tcgccgataa tgtagtcgta
     4801 gcgctcgccc gagagcacgg ccgaaccctg gccgtcgcgc accacgaccg gccgcaggaa
     4861 taccatcagg ttcgttttgc tgcgcgagcg cgtcttgtac tggaatagcg cgccgagcag
     4921 cgggatgtcg ccgagcagcg ggactttctc gacgccatcc tttaccgagt cctggatcag
     4981 cccgccgatc accacgatct gcccgtcgtc caccagcacc gtcgtctcga ccgcgcgctt
     5041 gttggtggtc ggccctgcgc tgggggtcac tatggtgtcg accacgctcg agacttcctg
     5101 gtaaatctgc agccgcacgg tgccgccctc ggagatctgg ggcttgatcc gcagcgtgag
     5161 gccgacgtcg cgccgctcga tggtctggac cggggtcggg gtgacggcgc cgccgg
//
LOCUS       DBNGSG010000288         5322 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2975573, whole genome shotgun sequence.
ACCESSION   DBNGSG010000288 DBNGSG010000000
VERSION     DBNGSG010000288.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5322)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5322)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5322
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2975573"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /gene="pyrH"
                     /locus_tag="AC158Q_07530"
     CDS             <1..578
                     /gene="pyrH"
                     /locus_tag="AC158Q_07530"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008652269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HIR3339666.1"
                     /translation="VAVVIGGGNIFRGIAPAASHMDRATADYMGMLATVMNALALQDA
                     MQRVNLVSRVQSALHIEQVVEPYIRGKAMRYLEEGKVVIFAAGTGNPFFTTDTAAGLR
                     GMEMNVDLVLKATKVDGVYTDDPKTHPDAMRYKRLSFDEAIIKNLKVMDATALTLCRD
                     QKLPINVFSIFKPGALKRVVLGEDEGTFLHN"
     gene            598..1158
                     /gene="frr"
                     /locus_tag="AC158Q_07535"
     CDS             598..1158
                     /gene="frr"
                     /locus_tag="AC158Q_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HIR3339667.1"
                     /translation="MMIADVKKTAEQKMKKTLETLKSDLGKVRTGRAHTGILDHVMVD
                     YYGTPTPIPQVANVTLIDARTVGVSPWDKKMSGAIEKAIRDSDLGLNPATMGETVRVP
                     MPALTEERRKELIKVVRHEGESARVAIRNVRRDANNHLKDLLKQKKVAEDEERRAQDD
                     VQKLTDRHIAEIDKLLQQKESDLMAI"
     gene            1158..1955
                     /gene="uppS"
                     /locus_tag="AC158Q_07540"
     CDS             1158..1955
                     /gene="uppS"
                     /locus_tag="AC158Q_07540"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HIR3339668.1"
                     /translation="MTGFSSSTLDIPDAHRVPRHIAIIMDGNGRWAKQRYLPRVAGHR
                     RGLDAVRTAVRACAERGVEFLTLFAFSSENWRRPADEISFLMQLFAMALEQEIEKLHE
                     NGIRFRSIGDLDRFEPKLGRLLREAEKLTAPNQRLTLTIAANYGGRWDILQAMNRMLR
                     ERPELASGFNERDLAPHLALSYAPEPDLFIRTGGEQRISNFVLWQLAYTELYFTDTLW
                     PDFDAAALDRAIVSYQQRERRFGRTSEQLPVDAGLATFRVGSTRTSD"
     gene            1990..2808
                     /locus_tag="AC158Q_07545"
     CDS             1990..2808
                     /locus_tag="AC158Q_07545"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HIR3339669.1"
                     /translation="MFTARLATAIALLVICISALLLLPNPWWTALLLPALLAASWEWG
                     GLMGLSHAARSLFVGFMVASLIMVWLLAAHFEPVAGRLTVPEVLIYGASCLFWCFIAL
                     VWLRRRWPLHSMLVLGAVGWIVLIPSWLVLARFQDSPGRLLAVLTIIWLADTAAYLSG
                     KAWGKHKMAPAVSPGKTWEGAAGAAAAVAVYYLILSRVVPEWGWWNGFGGAALFAGVA
                     VMSVVGDLFESAIKRQAGVKDSGTLLPGHGGVLDRMDSMTAALPVAALLLPYTG"
     gene            2818..4044
                     /gene="ispC"
                     /locus_tag="AC158Q_07550"
     CDS             2818..4044
                     /gene="ispC"
                     /locus_tag="AC158Q_07550"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017778143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HIR3339670.1"
                     /translation="MTGRQRLTILGSTGSVGVSTLDVVARHRDRFDVVALTARSRTEA
                     IFKQCLEFQPRIAVVQEATAAQDLRARLRQAGLDCEVRCGVESLEEVASLPDVDTVMA
                     AIVGIAGLRPTLAAAHAGKKVLLANKESLVTAGAVLMDAVRTNGATLLPIDSEHNAIF
                     QALPRGYSAPGTAGVRRILLTASGGPFRSATMRQLEDVTPDQACAHPNWAMGRKISVD
                     SATMMNKGLEVIEARWLFDVSPECIEVVIHPESVVHSLVEYIDGSVIAQLSNPDMRIP
                     IAFALGFPGRITAGVDYLDLARIGALHFERPDTERFPCLRLAYDALRAGGTAPTVLNA
                     ANEVAVASFLSGGLGFSQIPALIEAVLSDVPCNPVSGLEDVLTADHAARERADHWLFS
                     AGVATGTGLLRQAASS"
     gene            4056..>5322
                     /gene="rseP"
                     /locus_tag="AC158Q_07555"
     CDS             4056..>5322
                     /gene="rseP"
                     /locus_tag="AC158Q_07555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HIR3339671.1"
                     /translation="MTLIITIIAFVVALGCLIVIHELGHYAVARACGVKVLRFSVGFG
                     KPLWTRLLGQDATEWVIAAIPLGGSVKMLDEREGPVADEDLPRAFNRQSVWRRFAIVV
                     AGPAANFLLAIALYWVLFIHGIPGIRPVIGGPPPATAAHAAGFAAGDTLVRIAEEPVS
                     TWQDARWILLQHAVQKSVVMIEVRGAGDTINWRKLDLSALSPSDLDSDFLRALGLARL
                     QPKLKPVIGELVSGGAAQRAGLKAGDEVVAIDNQRIENWDQVVKIVRSQPGTPLVIEI
                     RRNGKLQPPVPVTPEVTVVNGQKTGRIGAAPRVDESTLAEMIVEVRYGPWESLVRAFA
                     KTWETSVFSLKMLGRMIMGDVSLKNLSGPITIADYAGQSAQSGLTSYLLFLALISISL
                     GVLNLLPIPLLDGGHLMYYTFEIINGRPIS"
BASE COUNT          970 a         1563 c         1766 g         1023 t
ORIGIN      
        1 aggtggcggt cgtcatcggc ggcggtaaca tatttcgcgg cattgcgccc gcggcttcgc
       61 acatggaccg cgcgaccgcc gactacatgg gaatgctcgc gacggtgatg aacgcgctgg
      121 cgctgcagga cgcgatgcag cgggtgaatc tcgtgagccg cgttcagtcc gcgctccaca
      181 tcgagcaggt ggtcgagccc tatatccggg gcaaggcgat gcgctacctg gaagaaggca
      241 aggtcgtgat attcgcggcg ggcaccggca atccgttttt caccactgac acggctgccg
      301 gtttgcgcgg catggagatg aacgtggatc tcgtgctcaa ggcgaccaag gtggacggag
      361 tttacacgga tgatcccaag actcaccccg atgcgatgcg ctacaaacgc ctgagcttcg
      421 acgaagccat catcaagaac ctgaaggtga tggacgcaac cgcattgacg ctgtgccggg
      481 atcagaaatt gccgatcaac gtcttcagca tcttcaagcc gggggcgctg aagcgcgtgg
      541 tactgggaga ggacgaagga actttcctgc ataattgaac ctgatcgcgg gaggtggatg
      601 atgattgccg acgtcaagaa aaccgccgag cagaagatga agaaaacgct ggagaccctg
      661 aagagcgatc tcggcaaagt ccgcaccggt cgcgcccata cggggattct cgatcacgtc
      721 atggtcgatt actatggcac gccgacgccg ataccccagg tggccaatgt gacgctcatc
      781 gatgcccgga cggtcggggt cagcccgtgg gacaaaaaaa tgtcgggtgc gatcgagaag
      841 gcgatacgcg attccgatct gggtctcaat ccggcaacga tgggggagac ggttcgggtg
      901 ccgatgccgg cccttaccga ggagcgccgc aaggaattga tcaaggtggt gcgccatgag
      961 ggcgaaagcg cgcgcgtggc aatacgcaat gtgcggcgcg acgccaacaa ccacctgaag
     1021 gatctgctca agcagaaaaa ggtggcggag gatgaggagc gccgcgcaca ggacgacgtc
     1081 cagaagctga ccgatcgcca catcgcggag atagacaagc tactgcagca aaaagaatct
     1141 gacttgatgg ctatctgatg actggcttca gcagctccac cctcgacata cctgacgcgc
     1201 accgcgttcc gcgccacatt gccatcatca tggacggcaa tgggcgctgg gccaagcagc
     1261 gctacctgcc acgagtggcg gggcatcggc gcgggctgga tgcagtgcgt acggccgtgc
     1321 gggcatgcgc cgagcggggt gtggaattcc tgacgctgtt cgcattcagc agcgaaaact
     1381 ggcgccggcc ggcggacgag atttcctttc tgatgcaact gtttgccatg gcgctcgagc
     1441 aggagatcga gaaacttcat gaaaatggaa tccgcttcag gtcgatcggg gatctggatc
     1501 gtttcgaacc caagctcggg cgcctgctcc gggaagccga gaagctgact gcccccaacc
     1561 agagacttac gctgaccatc gctgccaatt atggagggcg gtgggatatc ctgcaggcga
     1621 tgaaccgcat gctgcgcgag cggccggaac tcgcttccgg tttcaacgag cgcgacctgg
     1681 caccgcacct cgcgctgagt tacgcgccgg aacccgacct gttcatccgt accggtggcg
     1741 agcagcgcat cagcaatttc gtgctgtggc agctggccta caccgagctc tatttcaccg
     1801 acacgctgtg gccggacttc gacgctgcgg cgcttgaccg ggctattgtt tcctaccagc
     1861 agcgcgagcg ccgcttcggc cgcaccagcg agcagcttcc ggtcgacgcc gggctggcga
     1921 cgttccgggt cggcagcacc cgcacctctg actgagatcg cgcggcactc gacgcggatt
     1981 gccccgacca tgttcactgc gcgacttgcc acggcaatcg cactgcttgt catatgcatc
     2041 tctgctctgc ttttgctgcc aaacccgtgg tggacggcgc tgctgttgcc ggctttgctt
     2101 gccgccagct gggaatgggg cggcctgatg gggctgtcgc acgctgcgcg cagcttgttt
     2161 gtcggattca tggtggcttc attgatcatg gtctggctgc tcgcagccca tttcgagcca
     2221 gttgccggcc gtctcaccgt gccggaggtc ctgatctacg gcgcgagctg cctgttctgg
     2281 tgttttatcg cactggtgtg gctgcgccgc cgttggcccc tgcactcaat gctggtgctg
     2341 ggggccgtgg gctggatcgt tctgattccc tcctggctcg tgcttgcgcg ttttcaggat
     2401 agccccggcc ggctgcttgc cgtgctgaca atcatatggc tcgccgatac cgcggcctac
     2461 ctgagcggca aggcatgggg caaacacaag atggcgcctg ctgtcagccc gggtaaaacc
     2521 tgggagggcg cagcgggcgc agccgcagcg gtggcagtgt attatttgat actgtcccgc
     2581 gtggtgcccg agtggggctg gtggaacggt ttcggcggcg cggcattatt cgcgggcgtg
     2641 gcggtcatga gcgtggtggg tgacctgttc gaatcggcga tcaagcgcca ggcgggggtc
     2701 aaggacagcg gcacgctgct gcccgggcac ggcggggtcc tggaccgcat ggatagcatg
     2761 accgcagccc tgcccgtagc tgcgcttttg ctgccgtaca cgggctaggc acttttcatg
     2821 acgggcagac agcgacttac catactggga tccacgggca gcgtgggagt cagcacgctc
     2881 gatgtcgtcg cgcgtcaccg ggacaggttc gacgtagtgg cgctgaccgc gcgcagccgg
     2941 acggaagcca tcttcaagca gtgcctcgaa ttccagccgc gcattgctgt cgtgcaggag
     3001 gctacggcag cgcaagacct gagagcgcgg ctccggcagg ccggtcttga ctgcgaggtt
     3061 cgctgtggcg tggaatcgtt ggaggaagtc gcgagcctgc ctgacgtgga cacggtcatg
     3121 gcggccatcg tgggcatcgc cgggttgcgt ccgaccctgg ccgccgcgca tgccggcaag
     3181 aaggttctgc tggccaacaa ggaatccctg gtgacggcag gcgccgtgct gatggacgcg
     3241 gtacgtacga acggagcgac gctgcttccg atcgacagcg agcacaatgc catcttccag
     3301 gcgttgcccc gcggttactc cgccccgggt acggccggag tgcggcgcat cctgttgacg
     3361 gcatccggcg ggccattccg ctcggctacg atgcgccagc tcgaagacgt cacgcccgac
     3421 caggcttgcg ctcatcccaa ttgggcgatg gggagaaaga tttcggtgga ttcggcaacg
     3481 atgatgaaca aggggctgga agtaatcgaa gcccgctggc tgttcgatgt cagtcccgag
     3541 tgtatcgagg tcgtgataca cccggagagc gtggtgcatt ccctggtcga gtacatcgac
     3601 ggatcggtca ttgcgcaact gagcaatccc gacatgcgta tccccatcgc attcgcgctc
     3661 ggtttcccgg ggcggatcac cgcaggtgtg gactatctcg atctggcgcg catcggagcc
     3721 ctgcatttcg agcgtcccga cacggaacga tttccctgcc ttcggcttgc ctacgatgcg
     3781 ctcagggccg gcggcacggc tcccaccgtg ctcaatgccg ccaacgaggt cgcggttgcg
     3841 agcttccttt ccggcgggct cggattcagc cagatacctg ccctgattga agccgtgttg
     3901 tccgacgttc cctgcaaccc cgtatccggt ctggaagacg tattgactgc cgaccacgcc
     3961 gcgcgcgagc gcgccgacca ctggctgttc tcggcgggcg ttgccactgg cacgggacta
     4021 ttgcgccagg ccgcaagttc ctgagtggag cggcgatgac actgatcatt acaatcattg
     4081 cgttcgtggt cgccctgggc tgcctgatcg taattcatga gcttggccac tatgccgtgg
     4141 ctcgggcctg cggcgtaaaa gtgttgcgct tttcggtcgg attcgggaaa ccgctgtgga
     4201 cgaggctatt ggggcaggat gcgaccgagt gggtcatcgc cgccattcct ctcggcggtt
     4261 ccgtcaagat gctggacgag cgcgaggggc ccgtggcgga cgaagacctg ccgcgggcgt
     4321 ttaatcgcca gtcggtctgg cgtcgctttg ccatcgttgt tgcgggaccg gcggccaatt
     4381 ttctgctggc gattgcgctt tactgggtac tgttcattca cggaattccg ggaatcaggc
     4441 cggtgattgg agggcctcca cccgctaccg cagcgcacgc ggccgggttt gctgcaggcg
     4501 atacgctggt cagaattgca gaggagccgg tgtcgacgtg gcaggatgcg cgctggatac
     4561 tgctgcagca cgccgttcag aaatccgtgg tcatgatcga ggtgcgcggc gccggggaca
     4621 ctatcaactg gcgcaaactc gacctgtccg cgctgtcacc gtccgacctt gacagcgatt
     4681 tcctgcgcgc gctgggcctg gcgcgcctgc agccgaagtt gaagcctgtc atcggtgaac
     4741 tggtgagcgg gggggcggcc cagcgcgccg gcttgaaggc gggcgacgaa gtcgttgcaa
     4801 tcgacaatca gcgcatcgag aactgggatc aggtggtgaa aatcgtgcgc agccagcccg
     4861 ggacgccgct ggtcatcgag atcaggcgga acggaaaatt gcagccgccc gttccagtca
     4921 ctcccgaagt aacggtagtg aacgggcaga agaccggccg tatcggggct gcgccgaggg
     4981 tggacgaatc gacgctggcc gagatgatcg tcgaggtgcg ttatggtccg tgggagtccc
     5041 tggtgcgggc gttcgcgaag acctgggaaa cctcggtgtt cagcctgaag atgctcggca
     5101 ggatgatcat gggcgacgtt tcgctcaaga acctctccgg cccgataacc attgccgact
     5161 acgcggggca atcggcgcag agcggcctga cgtcgtactt gctgtttctt gcgctgatca
     5221 gcatcagcct cggtgtgctg aatctgctgc cgattccgtt actggatggc gggcatctaa
     5281 tgtattatac gttcgagatt atcaacggtc gtccgatctc gg
//
LOCUS       DBNGSG010000289         5799 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2467499, whole genome shotgun sequence.
ACCESSION   DBNGSG010000289 DBNGSG010000000
VERSION     DBNGSG010000289.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5799)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5799)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5799
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2467499"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="AC158Q_07560"
     CDS             complement(<1..845)
                     /locus_tag="AC158Q_07560"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3339672.1"
                     /translation="MKTLSALLLSVAMLAAVGAPAAETPKSGGLLKFAVGAEPPNYDC
                     HAQTSFAFIHPVRPHYNTLLKFDTAKYPAIRGDLAESWTVSKDGLTYTFRLRKGVKFH
                     DGSAFSSADIKATYERLRNPAPGVRSVRQATYADIASIDTPDPQTVVFKLKKPNSGML
                     ASFASPWDCVYSAAKLKQDPKFPERNIMGTGPFTFSGHVAGSHWTGRKFDGYFEKGKP
                     YLNGYVAIFIGGAPMVNALAAGEVLVEFRGHSPADRDRLVKALGDRATVQETPWVCSL
                     VVTFNT"
     gene            complement(936..3011)
                     /locus_tag="AC158Q_07565"
     CDS             complement(936..3011)
                     /locus_tag="AC158Q_07565"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3339673.1"
                     /translation="MDQPTALHGPAAPAPAQSPWLLEVEDLHVHFVTTRGVVRAVEGI
                     SYRVRPGETVALVGESGCGKSVSSLAIMRLLVKPAGRIVAGRILFEGRNLLELAEDQM
                     REIRGRDIAMIFQEPMTSLNPVLTIGFQIMEPLLIHLAMTEAAARARAVELLKMVGIP
                     DPERRLGQYPHQFSGGMRQRVMIAIALSCNPKLIIADEPTTALDVTIQAQILKLMKDL
                     SRDLGIAMVIITHNLGIVARYADRVNVMYAARLAEQGTAVAVFAKPLHPYTAGLLRSV
                     PRLDKPRGLKLETIDGLPPNLLDAPPGCRFAPRCPARQDACVAAPPPLIEAEPHHYSA
                     CVRAAELAQVGPTGLGLQSASPRPPETKSLDRTKPLLQVHDLCTYFEVGAGLQMLKSA
                     RVEVRAVDGVSFEVFRGETLGLVGESGCGKTTVGRTLLRLEQATSGEIVFEHANVTRA
                     AGKQLKDYRRKIQVIFQDPYSSLNPRMTIGEIIAEPMRVYRLVPDAKAAREKVAELLT
                     QVGLFEYMAERYPHELSGGQRQRVGIARALAMRPSFIVCDEPVSALDVSIQAQIINLL
                     EDLQQKYQLTFLFIAHDLAVVRHISDRVIVMYLGKVMEIADRDTLYADPLHPYTKALL
                     DAVPVPDPLIEAKREYRVLGGEVPSPLNPPRGCVFHTRCPLATDECKMAIPELREVRP
                     QHFAACIKI"
     gene            3291..3896
                     /gene="gstA"
                     /locus_tag="AC158Q_07570"
     CDS             3291..3896
                     /gene="gstA"
                     /locus_tag="AC158Q_07570"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HIR3339674.1"
                     /translation="MKLYYSPGACSLAVHIALRELKYKFDLDRVDLAAKKTESGGDYL
                     SINPKGYVPALQLDDGTMLTEVGVILQYLADQKPRRGLAPKPRTPERYKLMEWLTFIS
                     SEIHKALGDFFNPKMTPEWREAKLDRLGKRFDYLERALGSNEHLMGEFSIADPYLFTV
                     LNWAKRHDVDLANWPNLQSFIQRMADRNTVKKAMKAEGLAK"
     gene            4004..4780
                     /locus_tag="AC158Q_07575"
     CDS             4004..4780
                     /locus_tag="AC158Q_07575"
                     /inference="COORDINATES: protein motif:HMM:NF023984.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3750 domain-containing protein"
                     /protein_id="HIR3339675.1"
                     /translation="MKMPSALQLTGLLAAFLVVALLLVARGGAADWRTASREPVGLAP
                     DPAQTPEAVVQVYGARAVRWRGYFGVHTWVAVKPAGAPGYTVYELVGWRLRHSDTAVV
                     IHERLADGRWFGNEPELLADKRGPGVDALIARIDKAARDYPYAGEYSVWPGPNSNTFT
                     AWIARAVPELELDLPPTAIGKDYNGPRLIGPAPSGSGLQLSLFGLFGVVVSGVEGLEI
                     NVLGLTFGVNPFAPALKLPVFGRLGPVRLFQNPRIVNRES"
     gene            complement(4831..>5799)
                     /locus_tag="AC158Q_07580"
     CDS             complement(4831..>5799)
                     /locus_tag="AC158Q_07580"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3339676.1"
                     /translation="RIATASAVFLLTALAVSPAIAQKTDWPARPVRFIVPFPPGGTVD
                     PLARLVGARLTPVLGQQFIVDNRPGASGSLGTGIAAKATPDGHTFVFVFDTHAVNPAL
                     LRLPFDTMKDLAPVMLVSTAPYGIATHPGKPYRNLADVIRTAKGKPDTVTFGSVGSGS
                     LGHLMMMLVQQAGAFRVVHVPYKGGGPMTVDALGGNIDLAVGTVALLTPFATGGKLRV
                     VGVTSSQRSAALPEVPTVAEQGFPGFSALAWWGILTTAGTPAPVIDRFNAELVKVFAL
                     PDVRKQLVGTLGMDLIASSPGHLRKFVAAEIARWGKVVREHKIRAD"
BASE COUNT          953 a         1948 c         1904 g          994 t
ORIGIN      
        1 gtattgaagg tcaccaccag cgagcacacc cacggcgtct cctgcaccgt cgcccggtcg
       61 ccgagcgcct tcaccaggcg gtcgcggtcg gccggcgaat ggccgcggaa ttcgaccagc
      121 acctccccgg ccgcgagcgc gttcaccatc ggcgcgccgc cgatgaagat cgcgacatag
      181 ccgttcaggt acggcttgcc cttctcgaag tagccgtcga acttgcgccc ggtccagtgc
      241 gatcccgcca cgtgcccgga gaaggtgaac ggcccggtgc ccatgatgtt gcgctcggga
      301 aatttcgggt cctgcttgag cttcgcggcg ctgtagacgc agtcccacgg gctggcgaag
      361 ctcgccagca tgccggagtt gggtttcttc agcttgaaca ccaccgtctg cgggtcgggc
      421 gtgtcgatcg aagcgatgtc ggcataggtc gcctgccgca ccgagcgcac gcccggggcc
      481 gggttgcgca gccgctcata ggtcgccttg atgtccgcgg acgagaacgc cgagccgtcg
      541 tggaacttga cgcccttcct cagcctgaag gtgtaggtga ggccgtcctt cgacaccgtc
      601 cacgactccg ccaggtcgcc ccggatcgcg gggtacttcg cggtgtcgaa cttgagcagc
      661 gtgttgtagt gcgggcgcac ggggtgtatg aaggcgaacg acgtttgcgc gtggcaatcg
      721 tagttgggcg gctccgcgcc cacggcgaac ttgagcagcc cgccggactt gggcgtttcc
      781 gccgccggcg cgccaacggc agcgagcatc gcgacgctca gaagcagtgc agacagtgtt
      841 ttcataccct cctccttttt taataatgcg tgattggtga tgggtgattc gagcaaccgg
      901 ttcaacttcg actcacgact cacgattcac gattttcaga tcttgatgca tgcggcgaaa
      961 tgctgcggcc gcacttccct caattcggga atcgccatct tacactcgtc cgtcgccagt
     1021 gggcagcgcg tatggaacac gcagccgcgc ggcgggttca gcgggctcgg cacctcccct
     1081 cccagcacgc ggtactcgcg cttggcctcg atcaggggat cgggcaccgg caccgcgtcg
     1141 agcagcgcct tcgtataggg atgcagggga tcggcataga gcgtgtcgcg gtcggcgatc
     1201 tccatcacct tgcccaggta catcacgatc acgcggtcgg aaatgtgccg caccaccgcg
     1261 agatcgtgcg cgatgaagag aaaggtgagc tggtacttct gctggaggtc ctccagcagg
     1321 ttgatgatct gcgcctggat cgagacgtcg agcgcggaga ccggctcgtc gcacacgatg
     1381 aaggacggcc gcatggcgag cgcgcgcgcg atgcccacgc gctggcgctg gccgccggag
     1441 agctcgtgcg ggtagcgttc cgccatgtac tcgaacagcc cgacctgggt cagcagctcg
     1501 gccactttct cgcgcgccgc cttcgcatcg ggcaccagcc ggtacacccg catcggctcg
     1561 gcgatgatct cgccgatggt catgcgcggg ttcaacgagc tgtaggggtc ctggaagatc
     1621 acctggatct tgcggcggta gtccttcagc tgttttcccg cggcacgcgt gacgttggcg
     1681 tgctcgaaca cgatttcgcc cgaggtcgcc tgctcgagcc gcagcagcgt cctgccgacg
     1741 gtggtcttgc cgcaacccga ttcgcccacc agccccagcg tctcgccgcg gaagacctcg
     1801 aacgagaccc cgtccaccgc gcgcacctcg accctggcgc ttttcagcat ctggagtccg
     1861 gcgccgactt cgaagtaagt gcaaagatcg tgcacctgca gcagcggttt cgtccggtca
     1921 agcgacttgg tctcgggcgg gcgcgggctg gcgctttgca gacccagccc ggtcgggccg
     1981 acctgcgcca gttcggccgc gcgcacgcag gccgagtagt ggtgcggctc ggcctcgatc
     2041 aggggcggcg gggcggccac gcaggcgtcc tgccttgccg ggcagcgcgg cgcaaagcgg
     2101 cagcccggcg gcgcgtcgag caggttgggc ggcaggccgt cgatggtctc gagcttcagg
     2161 ccgcgcggct tgtcgagccg cggcaccgag cgcagcaacc ccgcggtata cggatgcagc
     2221 ggtttcgcga acacggccac ggcggttccc tgttccgcca ggcgcgcggc gtacatcacg
     2281 ttcacgcggt cggcgtagcg ggcgacgatg cccaggttgt gggtgatgat caccatcgcg
     2341 atgccgaggt cgcgcgagag gtccttcatg agcttgagga tctgcgcctg gatcgtcacg
     2401 tcgagcgcgg tcgtgggctc gtcggcgatg atcagcttcg gattgcacga gagcgcgatc
     2461 gcgatcatca cgcgctggcg catgccgccc gagaactggt gggggtactg gccgagccgg
     2521 cgctccgggt cgggaatgcc gaccatcttc agtagctcca ccgcgcgcgc gcgcgccgcg
     2581 gcttcggtca tcgcgaggtg gatcagcagc ggctccatga tctggaagcc gatggtgagc
     2641 acggggttca acgacgtcat cggttcctgg aagatcatcg cgatgtcccg gccgcggatc
     2701 tcgcgcatct ggtcttccgc gagctccagc aggttcctgc cctcgaacag gatgcggccc
     2761 gcgacgatgc gcccggcggg cttcacgagc agccgcatga tggcgagcga ggacaccgac
     2821 ttgccgcagc cggattcgcc gaccagggcc acggtctcgc cgggcctgac cctgtaggaa
     2881 atgccttcga ccgcgcgcac cacgccgcgc gtggtcacga aatggacgtg caggtcctcg
     2941 acctcgagca gccaggggct ttgcgcgggc gcgggcgcgg cggggccgtg cagtgcagtg
     3001 ggttggtcca tcctccgggg aaactgcttt ctggtagtgg gttcaggcta ttgaccacga
     3061 tgcggattgt caagtctccg caggaccgga cgcggcgcgc cggcctgcgt ccgcgcgttc
     3121 gaacggacgg aatttccgcc gctcaacgcg cagcagcggc gcgagaactt gccggaatag
     3181 acccggtcta aaccggctca ccgcgatagt tttgtagaat acggacgaag cttctcccat
     3241 tccccgtcac ttcgtcaccg cgtcagctca tcacttttcc gaaggaaccc atgaaactct
     3301 attactcccc cggcgcctgc tcgctggccg tgcatatcgc gctgcgcgag ctgaagtaca
     3361 agttcgatct cgaccgcgtc gatctcgcgg cgaagaagac cgagagcggc ggagattacc
     3421 tctcgatcaa tccgaagggc tacgtgccgg cgctccagct cgacgacggc acgatgttga
     3481 ccgaagtggg cgtcatcctc cagtacctcg ccgaccagaa gccgcgccgc ggccttgcgc
     3541 ccaagcccag aacgcccgag cgctacaagc tgatggaatg gctcaccttc atctcctccg
     3601 aaattcacaa ggcgctcggc gacttcttca acccgaaaat gacgccggag tggcgggaag
     3661 cgaagctcga ccgcctcggc aagcgctttg actatctcga gcgggcgctc ggctccaacg
     3721 agcacctcat gggcgaattc agcatcgcgg acccctacct gttcaccgtg ttgaactggg
     3781 cgaagcgcca cgacgtcgac ctggcaaact ggccgaacct ccagtcgttc atccagcgca
     3841 tggccgaccg caataccgtc aagaaggcga tgaaggccga agggctcgcg aagtgaggga
     3901 tgagggatga gggatgagga atacccgtca ctcgtcaccc gtcacccgtc acccgtcacc
     3961 cgtcacccgt cactcgtcac tcgtcactcg tcactaattt gaaatgaaaa tgccctccgc
     4021 gctccagttg accggcctgc tcgcggcctt ccttgtcgtg gcgctgctgc tcgtcgcgcg
     4081 cggcggcgcg gcggactggc gcacggcgag ccgcgagccg gtcgggctcg cgcctgaccc
     4141 cgcgcaaaca ccggaagccg tggtccaggt gtacggggcg cgcgcggtac gctggcgcgg
     4201 ctattttggc gttcacacct gggtcgcggt gaagccggcc ggcgcccccg gctataccgt
     4261 ctatgagctg gttggctggc gcctgcgcca ttccgatacg gcggtggtga ttcacgagcg
     4321 gctggccgac ggccgctggt tcggcaacga gccggaattg ctggcggaca agcgcgggcc
     4381 gggcgtggac gcgctgatcg cgcgcatcga caaggccgcg cgcgactacc cgtacgccgg
     4441 cgaatacagc gtctggccgg gaccgaattc caacacgttc accgcctgga tcgcccgcgc
     4501 ggtgcccgag ctcgagttgg acctgccgcc gaccgcgatc ggcaaggact acaacggccc
     4561 ccgcctgatc gggcctgcgc cgagcggcag cggcctgcag ctgtcgctgt tcggcctgtt
     4621 cggcgtggtg gtgagcggcg tcgaaggcct cgagatcaac gtgctcgggc tcaccttcgg
     4681 cgtgaacccc ttcgcccccg cgctgaaact gccggtcttc ggacgtctcg gacccgtgcg
     4741 cctgtttcag aatccgcgca tcgtcaaccg cgaatcgtaa aacgcaactg cgttctttcg
     4801 tttcacgatt cccgtttcgc gtttcacgag tcagtcggcg cgtatcttgt gctcgcgcac
     4861 gacttttccc cagcgcgcga tctcggcggc cacgaacttc cgcaggtgcc ccgggctgct
     4921 cgcaatgagg tccatgccca gcgtcccgac gagctgcttg cgcacgtccg gcagggcgaa
     4981 aaccttcacg agctcggcat tgaacctgtc gatgacgggt gcgggcgttc cggccgtcgt
     5041 caaaatgccc caccaggcca gcgcagagaa cccggggaat ccctgctccg cgaccgtcgg
     5101 cacttcggga agcgccgcgg agcgctggct gctggtgacg ccgacaacgc gcagcttgcc
     5161 gcccgttgcg aacggcgtca gcaacgccac cgtgccgacg gcgaggtcga tgttgccgcc
     5221 cagcgcgtcc acggtcatcg ggccgccgcc cttgtagggg acgtgcacga ccctgaatgc
     5281 gcccgcctgc tgcacgagca tcatcatgag gtggccgagg ctgccgctgc cgaccgagcc
     5341 gaaagtaacc gtgtcgggct ttcccttcgc cgtcctgatc acgtccgcca ggtttcggta
     5401 gggtttgccg ggatgggtcg cgataccgta gggcgccgtg ctgaccagca tgacgggcgc
     5461 gagatccttc atcgtgtcga acggcagccg caggagcgcc ggattcaccg cgtgcgtgtc
     5521 gaacacgaaa acaaacgtgt ggccgtcggg tgtggccttc gctgcgatgc ccgtgccgag
     5581 cgaacccgaa gctcccggcc ggttgtccac gatgaactgc tgccccagca ccggcgtcag
     5641 ccgggctccc accagccgcg caagcgggtc caccgtgccc ccgggcggga acggcacgat
     5701 gaatcggacc ggcctggccg gccaatcggt cttctgcgca atcgccggcg ataccgccag
     5761 cgcggtcaac aggaaaacgg cgctcgctgt cgcgatccg
//
LOCUS       DBNGSG010000290         1180 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1278657, whole genome shotgun sequence.
ACCESSION   DBNGSG010000290 DBNGSG010000000
VERSION     DBNGSG010000290.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1278657"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="AC158Q_07585"
     CDS             complement(<1..340)
                     /locus_tag="AC158Q_07585"
                     /inference="COORDINATES: protein motif:HMM:NF013293.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HIR3339677.1"
                     /translation="MSVQIAISGVSGRMGRALLEAVAADADCGLRAAIDRAGSPLVGQ
                     DAGAAWGAASGVRVTDQPASALQGAHALIDFTRPEATFGYLDACATAGVPMVIGTTGF
                     DEAGRARIAAA"
     gene            complement(337..798)
                     /locus_tag="AC158Q_07590"
     CDS             complement(337..798)
                     /locus_tag="AC158Q_07590"
                     /inference="COORDINATES: protein motif:HMM:NF016258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990063 - Bam protein complex [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HIR3339678.1"
                     /translation="MRHFLITALFLSLAAGCSGGKIGPYRIDVQQGNALDQESVARLK
                     PGLSRSQVRFLLGTPLVVDPFRTDRWDYVYLYHRAGKVTEQKRVTLFFDGETLVRIEG
                     DLPEPAERPPVAGPSGQPGAPAPVLNPKTNSDAMVPPATQEKLPTPEGVQP"
     gene            878..>1180
                     /locus_tag="AC158Q_07595"
     CDS             878..>1180
                     /locus_tag="AC158Q_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HIR3339679.1"
                     /translation="MSNPSDLKSIGLKATLPRLKILDLFEQSNKRHMTAEEVYRLLSD
                     EGQDIGLATVYRVLTQFEQAGILIRHHFESGKAVFELNQGGHHDHLVCIQCGQVEEF"
BASE COUNT          226 a          396 c          369 g          189 t
ORIGIN      
        1 ccgccgccgc aatgcgcgcg cgccccgcct cgtcgaagcc cgtggtgccg atcaccatcg
       61 gcacaccggc cgtggcgcag gcatcgagat agccgaaagt agcctcgggc cgggtgaaat
      121 cgatcagcgc gtgcgcgccc tgcaaggcag aagccggctg atcggtcacg cgcaccccgc
      181 tggccgcgcc ccatgcggcg ccggcatcct gccccaccag cgggctgcct gcgcggtcga
      241 tcgcagcgcg caggccgcag tcggcatcgg ccgcgaccgc ctcgagcagc gcgcgcccca
      301 tgcggccgga caccccggaa atggcaatct gcacactcat ggctggacac cttcaggggt
      361 cggcagcttc tcctgcgtgg cggggggcac catggcgtca ctgtttgtct tgggattcag
      421 caccggggcg ggcgcaccag gttgtccact cgggccggcc acaggaggcc gctcagccgg
      481 ttccggcagg tcgccctcga tgcgtaccag cgtttcaccg tcgaaaaaca gcgtgacccg
      541 cttttgttcg gtcaccttgc ctgccctgtg atacaggtag acataatccc aacggtcggt
      601 gcggaaagga tcgaccacca gcggcgttcc cagcaggaag cgaacttgcg aacggctcag
      661 gcccggcttc aggcgggcga cgctctcctg gtcgagcgcg ttgccctgct gcacatcgat
      721 gcggtagggg ccaatcttgc cgcccgagca gccggcagcc aggctcagaa acagcgcagt
      781 gatcaggaaa tgacgcatgt ccaggaaacc ggaagggttg gggcggttgg aaaaaaacgt
      841 atcataacgt attcgcgttc tcgttctgga gaccccattg agcaatccgt ccgatctcaa
      901 gagcatcggt ctcaaagcca cgctgccgcg cctgaaaatc ctcgatctgt tcgagcagag
      961 caacaagcgt cacatgaccg ccgaagaggt ctatcgcctg ctgtccgacg aaggccagga
     1021 tatcggactg gccacggtgt accgcgtgct cacccagttc gagcaggcgg gcatcctgat
     1081 ccgccaccat ttcgaatccg gcaaggcggt attcgaactc aaccagggcg gccaccacga
     1141 tcacctggtc tgcatccagt gcggacaggt cgaggaattc
//
LOCUS       DBNGSG010000291         3491 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2467951, whole genome shotgun sequence.
ACCESSION   DBNGSG010000291 DBNGSG010000000
VERSION     DBNGSG010000291.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2467951"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="AC158Q_07600"
     CDS             <1..932
                     /locus_tag="AC158Q_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339680.1"
                     /translation="PLHTEATHADTDFSQIGSGMNQPPNSEELQQILFAGLSQFWQAT
                     AMHRRLDMLVEQYWAAKREKSKNPLLKYGEKFFSQHDEDGILLEICKRIRLTRGVFVE
                     IGVGNGLENNSLILLMNGWQGVWLGAEPLAFDVPDSGPLLFQQAWVTRETCSALVTHG
                     LTALGQGQPNVLSVDIDGNDLYVVEAIIASGISPDIVICEYNAKFPPPIRWSINYDPA
                     HSWDGSDYQGVSLQLLVDVLGKYDYRLVACNVTGSNAFFVLNRHAGDFDDVPRSAAEL
                     FMAADYNWFFGRGHATSPRTIAGFLKPNGNEPE"
     gene            948..3275
                     /locus_tag="AC158Q_07605"
     CDS             948..3275
                     /locus_tag="AC158Q_07605"
                     /inference="COORDINATES: protein motif:HMM:NF025214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3339681.1"
                     /translation="MTAWPFTGESRHDALARHRNGLELLQRNQASLALAEFQQAVQLD
                     PDNAEFLKSRGNAERALGNLQGAVESYRRSLEAAPEYLPALYNMGLVLREMNRLEEAE
                     SYFRRVHELDPRDPDALFHLGALLHRRSQFADAAKSYRLALELAPGNPHLWLYLGKTY
                     GEIPAQLHEAIRCLRKCLELEPGLAEAHYQLGLAHKNLGRGEEAVACYRKTLELEPHS
                     AEAHDGLGNVLQEQGRLDEAIEHYREAIRLSPDSPVAHSNLGCALVRKGRLNEALRCF
                     LEAIDLHPDFAGAHLNLGNLHSLRGERDQALRCFDIALRIRPDDAAAREGLLFEMQHV
                     CDWSRFEELCTLQRRSLFGHPQQPVSPFSLLSIPSTPMEQLQCAKNYAERQLGAVARD
                     RERMTFRFERNAGRKTRVGYLSADFREHVVARLAVELFELHDRSRFEITAYSYGIDDG
                     SAMRARLSRAFDRFVDIGSLSYADAAARIHADQIDILVDLQGYTQHARTEIMALRPAP
                     VQVNFLGYTGTMGASFIDYAVVDRFVAPPGADAAFSEALVRLPESLQVNSRVRPMVET
                     PLRRNLGLPDKSFVFCCFNQTYKILPEIFAIWMRLLEAVPGSVLWLVESNAWATQNLR
                     REARSRGVDPDRLILAPTLPYERHLARLGAADLFLDTLPYNAHTSTSDALWVGLPVLT
                     CPGETFASRMAGSLLTSAGMPELIVRSLPEYETSALRLARTPGELSALREKLARNRST
                     APLFDTPGFVRYLESAYERMWHNYLAGNEPRRIDF"
BASE COUNT          683 a         1006 c         1075 g          727 t
ORIGIN      
        1 ttcccctaca cactgaggca acccatgctg atacggactt tagccagata ggatcgggta
       61 tgaaccagcc ccccaatagt gaagagttgc agcagatatt gtttgcaggg ctgtcgcaat
      121 tctggcaggc gaccgccatg cacaggcggc tcgacatgct ggttgagcag tattgggcgg
      181 caaaacggga aaaatcgaaa aatcccctgc tgaaatatgg agaaaaattc ttttcccaac
      241 acgacgaaga tggaattctt ctggaaatct gcaagcgaat ccgtttgacg cgcggcgtat
      301 tcgtggagat cggtgtaggc aacggcctgg agaataactc cctgatattg ctcatgaatg
      361 gctggcaggg tgtctggcta ggcgctgaac cgctcgcgtt tgacgtgccg gattccgggc
      421 ctttgctgtt tcaacaggca tgggtgacca gggaaacctg tagcgcgctc gtgacccatg
      481 ggctgaccgc gctcggccag gggcagccga acgtgctctc ggtggatatt gatggcaacg
      541 acctgtatgt cgtcgaggcg atcatagcgt cgggcatttc tcctgatatc gtcatttgcg
      601 agtacaacgc gaaatttcct cctccgatac gctggtcaat caactatgac cctgcgcaca
      661 gttgggatgg ttccgattat cagggtgtat ccctgcaatt gctggttgat gttcttggca
      721 aatacgatta caggctggtt gcctgtaatg taaccggctc aaacgctttc ttcgtgttga
      781 accggcacgc gggggatttt gacgatgttc cgcgcagcgc agcggagctt ttcatggctg
      841 cggactacaa ctggtttttt ggccgcgggc atgcaacgtc gccgcggacc atcgccggtt
      901 ttctcaagcc caacggaaat gaaccggaat aaggaatctt ccgagccatg acggcgtggc
      961 cattcacggg agagtcgcgt cacgatgcgc tggcgcgcca ccgcaacggg ctcgagttgc
     1021 tgcaacgcaa tcaagcgtcc ctcgcgctgg cggagttcca gcaggcggta caactggatc
     1081 ccgataatgc cgagtttctc aagagccgcg gtaacgcgga acgagctttg gggaatttgc
     1141 agggtgccgt tgagagttat cgtcgttcgc ttgaggcggc gccggaatac ctgccagccc
     1201 tttacaacat gggcctggtc ctgcgtgaga tgaaccggct cgaggaggcc gagagttatt
     1261 tccgcagagt tcacgaactg gatccgcggg atccggacgc gctttttcac ctgggtgcgc
     1321 tgctccaccg gcgctcgcag tttgcggacg cggcgaagag ttaccgcctg gcgctggagt
     1381 tggcgccagg caatccccac ctgtggttgt acttggggaa aacctatggg gagataccgg
     1441 cgcagctgca cgaggccatc cggtgcctgc gaaaatgcct ggaactcgaa ccaggccttg
     1501 cggaagccca ttatcagctc ggcctggccc acaagaacct gggcagaggc gaggaggcgg
     1561 ttgcctgcta ccgcaagacg ctggaactgg agccgcactc tgccgaggcg cacgacggtc
     1621 tgggcaatgt acttcaggaa cagggccgcc tggacgaggc catcgaacat tatcgggagg
     1681 cgatccggct gtctccggat tctccagtgg cacacagcaa cctcggctgc gcgctcgtgc
     1741 gcaagggccg gctgaatgag gcgctgcgat gttttcttga agccattgat ttacaccctg
     1801 attttgccgg cgcccatttg aatctcggca atcttcacag ccttcgcggc gagcgcgacc
     1861 aggcgctacg ctgctttgac atcgcgttgc ggattcggcc ggatgacgcc gccgccaggg
     1921 aaggcctgct gttcgagatg cagcatgtgt gcgactggtc gcgctttgag gagctttgca
     1981 ccctgcagcg ccggagcctt ttcggccacc cgcaacagcc tgtcagccca ttcagcctgc
     2041 tatcgatacc ttcgactcca atggagcagt tgcaatgcgc aaagaactac gcggaacggc
     2101 aactgggtgc cgttgcaagg gaccgcgaac gcatgacatt tcgcttcgag cgaaatgccg
     2161 ggcgcaagac aagggtcggg tatctttccg ccgattttcg cgagcacgtc gtcgcccgcc
     2221 tggcggtgga actcttcgaa ttgcacgacc ggagccgctt tgaaattact gcctactcgt
     2281 acgggatcga cgacgggagc gcgatgaggg cgcgcctatc ccgcgcgttc gatcgtttcg
     2341 tggacattgg gtccctgtcg tacgcggatg cggcggcgag gatccatgcc gatcaaatcg
     2401 acatactggt agacctgcag ggatacacgc aacatgcccg gaccgagatc atggcgttga
     2461 ggcccgctcc cgtccaggtg aactttctgg gctacacggg aacgatgggc gcatccttca
     2521 tcgactacgc cgtggtggac cggtttgtcg cgcccccggg cgcggacgct gctttcagtg
     2581 aggcgctggt ccggctgccg gagagcctgc aggtgaatag ccgcgtgcgg ccaatggtcg
     2641 aaacaccgtt gcgccggaac ctgggtttgc cggataagtc atttgtcttt tgctgtttca
     2701 atcagaccta caagatactg ccggagatct tcgcgatatg gatgagattg ctcgaggcgg
     2761 ttccgggcag cgtgctgtgg ctggtcgaga gcaatgcctg ggcgacgcaa aaccttcgcc
     2821 gcgaggcccg cagccggggc gtcgatcctg accggttgat tctcgcgccg acactgccat
     2881 acgaacgtca ccttgcccgg ctgggggccg ccgacctgtt tctcgataca ctgccctaca
     2941 acgcgcatac gagcaccagc gacgcgctgt gggtagggtt gccggtcctg acctgcccgg
     3001 gggaaacctt cgcgtcgcgc atggccggca gcctgctcac ttcggcaggc atgccggaac
     3061 tgatagtgcg gtcgctgccg gaatacgaaa cgtctgcgct acgcctcgcg cgtacgcccg
     3121 gggaactgag cgcgctgcgc gagaaacttg cccggaaccg gtcgaccgcg cccttgtttg
     3181 acacgcccgg ttttgtgcgc tacctcgagt cggcgtacga gcggatgtgg cataactatc
     3241 tggcaggaaa tgagccgcgc cgaatcgatt tctaggcacg caggatcgcc tcccggccag
     3301 cggatgcgcg catgaaccgt ccacgtgccg atatttgtca gtaatcgacg gttcaggaca
     3361 cctgtctcgc ccccacgcct ggagtcccat caccgatgtc gagggtttat tgtaacttgc
     3421 tgacttctgg acagattagt tcagtcatta cctggcccgt ttcctgctag ggtgaaaccc
     3481 gaatattcag c
//
LOCUS       DBNGSG010000292         2535 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3318265, whole genome shotgun sequence.
ACCESSION   DBNGSG010000292 DBNGSG010000000
VERSION     DBNGSG010000292.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3318265"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1196
                     /locus_tag="AC158Q_07610"
     CDS             <1..1196
                     /locus_tag="AC158Q_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIR3339682.1"
                     /translation="LSKSLVSDVKARSAHDAAALIAEHPAATIAPILMELNPGFAQDI
                     LAELPGGLKDAVLHAIPPEMALQWQRNQTYEQASIGRLMEPAYAVFHPGMTVAQTVER
                     LRILLKVAFITYGYVIEEGGKLAGLITMRDLLFANEGERLETLMMRNVFTLNPKTPLG
                     EAMKLVLERHYPVYPVCDENGILLGLVRGQAMFEEQAFEITAQAGTMVGVEKEERLAT
                     PLKQSFKFRHPWLQLNLVTAFVAGAVVAIFQDTVDRLVILAVFLPILAGQSGNTGCQA
                     LAVTLRGMTLGELKGGKERALVTKEAWLGFYNGSLTGLVAGTGMLVMAIYQDNPNAIA
                     LAFVVWLALVGACVVSGICGALIPITLKRMNLDPATASSIFLTTATDVVSMGMLLGLA
                     AFLIS"
     gene            complement(1330..>2535)
                     /locus_tag="AC158Q_07615"
     CDS             complement(1330..>2535)
                     /locus_tag="AC158Q_07615"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005882785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HIR3339683.1"
                     /translation="QRTLMGPGPTEIHPRVLTTMSQPAIGYLDPVFIEMMEELKTLLR
                     YVYQTKNPLTFPVSGPGSVGMEYCFVNLVTPGAKVIVCRNGVFGGRMIENVERCGGTA
                     VVVEDKWGEPIDPNKLEDALKKNPDVRLVAFVHAETSTGVQSDARKLIEIAHKHDALT
                     IVDAVTSLGGTPVLVDEWKVDAIYSASQKCLSCTPGLSPVSFSERVVEHVKARKDKIH
                     SWFMDMNLLLGYWGATNRTYHHTAPTNSLFALHEALLLINEEGIENSWARHQRHHVAL
                     KAGLEAMGLKFLVKPEYQLPQMNAVFCPEGVDEAKVRRTLLTEFNLEIGAGLGPLAGK
                     IWRFGLLGYSCRPDNVMLCLSALGSVLEDMGYAVHVGDAEAAAHAAYAAMHAKAAQAK
                     AKTRTKAAA"
BASE COUNT          416 a          845 c          824 g          450 t
ORIGIN      
        1 ccctgagcaa gtcgctggtg tccgacgtca aggcgcgctc cgcgcacgac gcggcggcat
       61 tgatcgcgga acatccggcg gcaacgatcg cgcccatcct gatggaattg aatcccggct
      121 tcgcgcagga catcctcgcg gagttgccgg gcgggctgaa ggacgcggtg ctgcatgcga
      181 ttccgcccga aatggcgctc cagtggcaac gcaaccagac ctacgagcag gccagcatag
      241 ggcggctgat ggagcccgcg tacgccgtgt tccacccggg gatgaccgtt gcccagaccg
      301 tggagcggct gcggatcctg ctcaaggtcg cgttcatcac ttacggctat gtgatcgagg
      361 aaggcggcaa gctggccggg ctgatcacga tgcgggacct gctgttcgcc aacgaaggcg
      421 agcggctcga gacgctgatg atgcgcaacg tgttcacgct caatccgaaa acgccgctgg
      481 gcgaggcgat gaagctggtg ctcgagcgcc attacccggt ctacccggtg tgcgacgaga
      541 acggcatcct gctcggcctg gtgcggggcc aggccatgtt cgaggagcag gccttcgaga
      601 tcaccgcgca ggccggcacc atggtcggcg tggaaaagga ggagcggctc gccacgccgt
      661 tgaagcagag cttcaagttc cgccacccct ggctgcagct gaacctcgtg accgcgttcg
      721 tcgccggtgc ggtcgtcgcc atcttccagg acacggtgga ccggctcgtg atactcgccg
      781 tgttcctgcc catactcgcc ggtcaatccg gcaacaccgg ctgtcaggcg ctcgccgtca
      841 cgctgcgcgg catgacgctg ggcgaactga agggcgggaa ggaaagggcg ctggtgacga
      901 aagaagcctg gctcggcttc tacaacggct cgctgaccgg cctcgtcgcg ggcacgggca
      961 tgctggtcat ggcgatctac caggacaatc cgaacgccat cgcgctcgcg ttcgtggtgt
     1021 ggctggcgct ggtcggcgcc tgcgtcgtca gcggcatctg cggcgccctg attccgatca
     1081 cgctgaagcg catgaacctc gatcccgcca ccgcctccag catcttcctc accaccgcca
     1141 ccgacgtggt cagcatgggc atgctgctgg ggctcgccgc ctttcttatc agctgacgcg
     1201 gaaagccgca ggcgctcgct ccggctgcca aaaacaggcc ggagtgatta ctaacttact
     1261 gcccatttcc cctatgaaaa cccccaccct gtccctcccc ctggactagg gggaggggtc
     1321 ggggacggct caggcggcgg ctttcgtccg ggtcttggct ttcgcctgcg ccgccttggc
     1381 gtgcatggcc gcataagccg cgtgcgcggc ggcctccgcg tccccgacgt gcacggcata
     1441 ccccatgtcc tcgagcaccg agccgagcgc cgacaggcac agcattacgt tgtccggccg
     1501 gcaggaatag ccgagcagcc cgaagcgcca gatcttgccc gcgagcggac cgagccccgc
     1561 cccgatttcg aggttgaact cggtgaggag cgtcctgcgc accttggctt cgtccacgcc
     1621 ttccgggcag aacacggcgt tcatctgcgg cagctggtac tccggcttca ccaggaactt
     1681 caggcccatc gcctccaggc cggccttcaa cgcgacgtgg tggcgctggt ggcgcgccca
     1741 cgaattctcg atgccttcct cgttgatcag cagcagcgcc tcgtgcagcg cgaacagcga
     1801 gttggtcggc gcggtgtggt ggtaggtgcg gttggtggcg ccccagtagc ccaacagcag
     1861 gttcatgtcc atgaaccagg aatggatctt gtccttgcgc gctttcacgt gctcgaccac
     1921 gcgctcggaa aatgacaccg gcgacaggcc cggcgtgcag gacaggcatt tctggctcgc
     1981 cgagtagatc gcgtccactt tccactcgtc caccagcacg ggcgtcccgc cgagcgaggt
     2041 caccgcgtcc acgatggtca gcgcgtcgtg cttgtgcgcg atctcgatca gttttctcgc
     2101 gtcggactgc acgccggtgg aggtctccgc gtgcacgaag gccaccaggc gcacgtcggg
     2161 attctttttc agcgcgtctt cgagcttgtt cgggtcgatc ggctcgcccc acttgtcctc
     2221 gaccacgacc gccgtgccgc cgcagcgctc gacgttctcg atcatgcggc cgccgaatac
     2281 gccgttgcgg cacacgatca ccttggcgcc cggcgtcacc aggttgacga agcagtactc
     2341 catcccgacc gagccggggc ccgagaccgg gaaggtgagc gggttcttcg tctggtagac
     2401 gtagcgcagc agcgtcttca gctcctccat catctcgatg aagacggggt cgaggtagcc
     2461 gatcgccggc tggctcatgg tggtgagcac gcgcgggtga atttccgtgg gccccggccc
     2521 catgagggtg cgttg
//
LOCUS       DBNGSG010000293         3238 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4506884, whole genome shotgun sequence.
ACCESSION   DBNGSG010000293 DBNGSG010000000
VERSION     DBNGSG010000293.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4506884"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..1047
                     /locus_tag="AC158Q_07620"
     CDS             202..1047
                     /locus_tag="AC158Q_07620"
                     /inference="COORDINATES: protein motif:HMM:NF025598.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HIR3339684.1"
                     /translation="MRRSMWLNALLAAAVAVLGAWAYFKSPRDAPSGHALSALKPSEA
                     ASIHIERPGAAPISLEKKAGAWVMTAPFAARGDESRVQRLLEILEAKSAHRLAATDLS
                     RFELDPPQARVTIGRQAFSFGMVNAVTREQYLLTNGTVFALHPNYGTALPAHPGEMAS
                     PRLLGSTEKPVRIELIAFTVEQRNGKWTQMPAAKDPSQDDFLRWVELWQLSPAARVEP
                     YVKGKLLEAIRIQLKDGATLALGVLAREPELVLLRADEKLQYHFRAELAKRLLSPPGT
                     AVKEK"
     gene            1097..1915
                     /gene="mutM"
                     /locus_tag="AC158Q_07625"
     CDS             1097..1915
                     /gene="mutM"
                     /locus_tag="AC158Q_07625"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HIR3339685.1"
                     /translation="MPELPEVETTRRGLEPLLVGQRIRAAIVRDRRMRQPVPRKLPQL
                     LAGATIRGLARRGKYLLADCGAGTLILHLGMSGRLWVVRDGEPPAPHDHFDLVLENGT
                     VVRLRDPRRFGLVLWQPGDPLTHALLANIGPEPLSTGFGGDTLHAATRGRSSAIKHVL
                     MDSRVVAGVGNIYANEALFHAGISPRTAAGKLTRARCTVLAEKIRETLELAIRAGGSS
                     LRDYVRSDGMAGNFQSQFMVYDRAGGPCQRCGTSIREIRQGQRSTFYCPKCQKS"
     gene            complement(1964..2215)
                     /locus_tag="AC158Q_07630"
     CDS             complement(1964..2215)
                     /locus_tag="AC158Q_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003066028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HIR3339686.1"
                     /translation="MALKITDECINCDVCEPECPNDAISQGEEIYVIDPHKCTECIGH
                     YPDPQCVKVCPVDCIPLDPDFVETKEELHAKYLKLTEKA"
     gene            complement(2411..2905)
                     /gene="coaD"
                     /locus_tag="AC158Q_07635"
     CDS             complement(2411..2905)
                     /gene="coaD"
                     /locus_tag="AC158Q_07635"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006760399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HIR3339687.1"
                     /translation="MNHKAVYPGTFDPITLGHEDLIRRAARLFDTVIVAVADSRAKRP
                     FFTRDERVDMARRVLKDLPNVEVVGFAGLLMKFIRSHKARVVIRGLRAVSDFEHEFQL
                     AGMNRGMYPDVETLFLTPGEQYMFVSATIVREISILGGDVRKFVPPLVARRLKEKFKQ
                     LGGK"
     gene            complement(2902..>3238)
                     /locus_tag="AC158Q_07640"
     CDS             complement(2902..>3238)
                     /locus_tag="AC158Q_07640"
                     /inference="COORDINATES: protein motif:HMM:NF015559.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HIR3339688.1"
                     /translation="SVVMVENDAVVYRALAASREALAAAAVELRKADALEFLRSDAGV
                     YDVVFADPPFKAGYWPRFAPLLPPRLAPGALVYYEGAAPPELPQGWEAVRQGRAGQVS
                     YQLLRWIQP"
BASE COUNT          563 a         1083 c         1010 g          582 t
ORIGIN      
        1 gccctctatc tcatcgcgtt cagcttcctg ttcgtgctgc cgcttgcctt catgctcacc
       61 ggcgcggtga tctggtggcg caggcgaagg atatgagccg ggccctatcg atgcccgcgg
      121 taatgaaaag aactccattt ccgcgccgta cattttccct ctccccccgg gacagggcga
      181 aggggtgagg gccgagcggc gatgcgacgc tcgatgtggc tgaacgcgct gctcgccgct
      241 gctgtcgccg tgctgggcgc ctgggcttat ttcaaatcgc cgcgcgacgc gccctccggg
      301 catgcgctgt ccgcgttgaa accctccgag gccgcatcca tccatatcga gcgcccgggc
      361 gcggcgccca tctccctgga aaaaaaagcg ggtgcctggg tgatgaccgc gccgtttgcc
      421 gcgcgcggcg acgaatcgcg ggtgcagcgg ctgctcgaga tcctcgaggc aaaatccgcc
      481 caccggctgg ccgctaccga cctctcgcgc ttcgagctcg atccgccgca ggcgcgcgtg
      541 acgatcggca ggcaggcttt cagcttcggc atggtgaacg cggtgacgcg cgagcaatac
      601 ctgttgacga acggcaccgt gtttgccctg cacccgaact acggcacggc gctgccggcg
      661 catcccggcg agatggcgag tccccgcctg ctcggttcca ccgagaaacc ggttcgcatc
      721 gagttaattg cgttcacggt ggaacagcgc aacggcaagt ggacgcaaat gcccgcggcg
      781 aaggatccca gccaggatga tttcctgcgc tgggtggagc tgtggcagct cagcccggca
      841 gcgcgcgtcg aaccctacgt caagggcaag ctgctggagg ccatccgcat ccagttgaag
      901 gacggcgcca cgctcgcgct cggcgtgctc gcgcgcgagc cggagctggt gctgctgcgc
      961 gcggacgaga agcttcagta tcacttccgc gccgagctgg cgaagcgcct gctctcgccg
     1021 cccggcaccg ccgtgaagga aaagtgatgt cctgtcaccc gccacccgtc actcgtcgct
     1081 cgccaccagc ccgggcatgc ccgaattacc ggaagtagaa accacccggc gcggcctgga
     1141 gccgctgctc gtcggccagc gcatccgggc tgcgatcgtc agagaccggc ggatgcgcca
     1201 gcccgtgcca cgcaagctgc cgcagctcct cgcgggcgcc acgatccgcg gcctcgcgcg
     1261 gcgcggcaaa tatcttctgg cggattgcgg cgccggcacg ctgatcctgc acctcggcat
     1321 gtcgggacgg ctgtgggtgg tacgggacgg cgagccgccg gcgccgcacg atcactttga
     1381 tctggtgctg gagaacggga cggtggtgcg gttgcgcgac ccgcgccgct tcggcctggt
     1441 gctgtggcag ccgggtgatc ccctcaccca cgccctgctc gcgaacatcg gccccgagcc
     1501 gctctcgacc ggattcggcg gcgacacgct gcacgcggcg acgcgcggcc gcagctcagc
     1561 catcaagcac gtgctgatgg acagtcgcgt ggtcgcgggc gtgggcaaca tctacgccaa
     1621 cgaagcgctg tttcacgccg gcatcagccc gcgcaccgcg gcaggcaagc tcacccgcgc
     1681 ccgctgcacc gtgctcgcgg agaaaatccg cgagacgctc gagctggcaa tacgcgccgg
     1741 cggcagcagt ctcagggatt acgtgcgcag cgacgggatg gccggcaact tccagagcca
     1801 gttcatggtc tacgaccgcg cgggcggacc ctgccagcgc tgtggaacat ccattcggga
     1861 gatccggcag ggacagcgct cgacgttcta ctgccctaaa tgccagaaat cgtgattcgt
     1921 gaatcgtgaa tggacctgaa aaccggggtc cccgaatcac gattcacgct ttttctgtca
     1981 gcttgagata cttcgcgtgc agctcctcct tcgtctccac gaagtcgggg tcgagaggta
     2041 tgcagtcgac cgggcacact ttcacgcact gcgggtcggg gtaatggccg atgcactcgg
     2101 tgcacttgtg cgggtcgatg acgtagatct cctcgccctg cgagatcgcg tcgttcgggc
     2161 actccggctc gcacacgtcg cagttgatgc attcgtcggt gatcttcagc gccatctgca
     2221 acctgaatag acaggattca caatagaacg ttatgacccg tcaaacccga aaatagacag
     2281 gattaacaag atttgcagga ttggatcaaa gacagaatct cgacgttact tttcttgtcg
     2341 gtattgatct tgtcaatcct gttaatcctg tccatcttga tcttgatcta atctgcggtt
     2401 gatcctgtca ttatttgcca cccagttgct tgaacttttc cttgagccgc cgcgccacga
     2461 gcggcggcac gaacttcctc acgtcgccgc cgaggatcga aatttcgcgc acgatggtcg
     2521 cggacacgaa catgtactgt tccccgggcg tcaggaacag ggtttcgaca tcgggataca
     2581 tgccgcggtt catcccggcg agctggaatt cgtgctcgaa gtccgatact gcgcgcagtc
     2641 cgcggatcac cacgcgcgcc ttgtggctcc ggatgaattt catcagcagc ccggcgaacc
     2701 cgacaacctc gacgttgggc aggtctttca gcacccgccg cgccatgtcc actcgctcgt
     2761 cgcgggtaaa aaacggccgt ttggcgcgcg agtcggccac cgccacgatt accgtgtcga
     2821 acagccgcgc cgcgcgccgg atcaggtctt cgtggccgag ggtgatcgga tcgaacgtcc
     2881 ccgggtagac tgccttgtgg ttcatggttg tatccatctc agtaattggt agctcacctg
     2941 ccccgcccgg ccctgcctga ccgcttccca tccctgcggc agttcgggag gcgccgcccc
     3001 ctcataatag accaatgccc ccggcgcgag gcgcggcggc agcagcgggg cgaagcgcgg
     3061 ccagtaacct gccttgaacg gcgggtcggc aaagacgacg tcatagacgc ccgcgtccga
     3121 gcgcaagaac tccagcgcat ccgctttcct gagctccact gcggcagcgg cgagcgcctc
     3181 gcggctcgcg gcgagcgcgc ggtagaccac cgcgtcgttc tccaccatga ccacgctg
//
LOCUS       DBNGSG010000294         3423 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4678154, whole genome shotgun sequence.
ACCESSION   DBNGSG010000294 DBNGSG010000000
VERSION     DBNGSG010000294.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4678154"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..1265
                     /gene="purD"
                     /locus_tag="AC158Q_07645"
     CDS             3..1265
                     /gene="purD"
                     /locus_tag="AC158Q_07645"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HIR3339689.1"
                     /translation="MGSGGREHALAWKLARCPRISRVLVAPGNAGTAREDGLANVAAS
                     SIPEWIDIARRETVAFTVVGPEAPLAEGIVDAFQAAGLKIFGPVQAAARLESSKEFAK
                     SFMQRHGIPTAAYAAFSEAAPAHDYIDRQGAPIVIKADGLAAGKGVVVAMSAAEAHAA
                     VEMMLTEKKMGDAGGRVVVEEFLDGEEASFIVLADGHHALALATSQDHKRLQDGDRGP
                     NTGGMGAYSPAPLVTPTIHARVMREVIQPALTGMEKDGTPYSGFLYAGLMIGKEGRIK
                     VLEFNCRMGDPETQPIIMRLKSDLVSLLEHALAGTLDRAEAEWDRRVALSVVLAAHGY
                     PDAPRKGDVIHGLPALEEDCHVFHAGTALDGKDVIVNGGRVLAVTALGGNVSAAQRRA
                     YEIAEKIHFDGMQFRRDIGHRALAGGRR"
     gene            1284..2138
                     /locus_tag="AC158Q_07650"
     CDS             1284..2138
                     /locus_tag="AC158Q_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339690.1"
                     /translation="MNANYDRARQDLGNIVLLEHVNVTIPDQRFATIFYLSGLGLTRD
                     PYLMTGIDNMWVNVGRHQFHLPSRGIQVLRGQVGIVMSDLDALKRRLQKVAPLLAETR
                     FAWEERDGAIETTCPWGNRDRGHAPAPEFGDTELAIAYVAFDVPPGAARGIARFYEEI
                     LGAHAALEGDTAIISVSATQRLRFRDTARPLPEYDGHHVQIYVSDFSGPHRRLLERGL
                     VTEESSEHQYRFRDLVDPASGKALFAIEHEVRSLTHPLYARPLVNRNPAQTNRDYTRG
                     QDAFRGTY"
     gene            2234..3223
                     /locus_tag="AC158Q_07655"
     CDS             2234..3223
                     /locus_tag="AC158Q_07655"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3339691.1"
                     /translation="MPVPFRIAPRFLTAVISATALLAFSHATPAQEAFPGKRPIEFVV
                     LFPAGSSADVTARVLAEGMGRELGTQIVVVNRPGGGGAVGYKYAAGQAADGHHLVWNS
                     NSISTTYHTGMLAFDYRSFAPVARVQVETPLLFVRGGAPWKSLRDLLQYTRANPGKLN
                     VGNSGTGSHTHFSSVWLFRTAGAQIVDVPFNAAQVVPGLLGGHVEAIMQLPGALAGHV
                     QAGTVRALVALSGARDPFFPDVPTAREQGVDAVAELWRGVAVPRGTPTRVVARLEDAI
                     RKTVTSPEFARATARLLVTPAFLPAAGFGKLIAKEDAEIAQAVKALGLRPSPK"
     gene            3258..>3423
                     /locus_tag="AC158Q_07660"
     CDS             3258..>3423
                     /locus_tag="AC158Q_07660"
                     /inference="COORDINATES: protein motif:HMM:NF013390.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HIR3339692.1"
                     /translation="MDVAAVKEFLVGLQQTIVTRLAEAGGQAFRRDEWIRPGGGGGIT
                     RMLEEGAVFER"
BASE COUNT          578 a         1132 c         1171 g          542 t
ORIGIN      
        1 tcataggttc aggaggacgc gaacatgcgc tggcgtggaa actcgcgcgc tgcccgcgca
       61 tctcgcgggt gctcgtcgcg cccggcaatg ccggcaccgc tcgcgaggac gggctcgcca
      121 acgtcgccgc gtcctcgata cccgagtgga tcgatattgc aagaagggaa acggtcgcat
      181 ttaccgtcgt tggcccggag gcgccgctcg ccgagggcat cgtggatgcg ttccaggcgg
      241 cgggattgaa gatcttcggg ccggtccagg ccgcggcgcg gctcgagagt tccaaggagt
      301 tcgccaagag tttcatgcag cgccacggga taccgaccgc agcctatgcg gcgttcagcg
      361 aggcggcgcc cgcccacgac tatatcgaca ggcagggcgc gcccatcgtc atcaaggccg
      421 acgggctggc cgcaggcaag ggcgttgtcg tggcgatgag cgccgccgaa gcgcacgcag
      481 cggtggaaat gatgcttacc gagaagaaaa tgggcgacgc cggcggccgg gtggtggtcg
      541 aggagttcct ggacggggaa gaggcgagct tcatcgtgct cgccgacggc caccacgcac
      601 tcgcgctcgc gaccagccag gaccacaagc gattgcagga cggcgaccgc gggcccaata
      661 ccgggggcat gggcgcctat tcaccggcgc cactggtaac gcccaccatc cacgccaggg
      721 tgatgcgtga agtgatccag ccggcgctca ccgggatgga gaaggacggc acgccctaca
      781 gcggatttct ctacgctgga ttgatgatcg gcaaggaagg gcggatcaag gtgctggaat
      841 tcaactgccg catgggcgac cccgaaacgc agccgatcat catgcggttg aagagcgacc
      901 tcgtgtcgct cctcgagcac gcgctcgcgg gcaccctcga cagggccgag gccgaatggg
      961 accggcgggt cgccttgagc gtcgtgctcg ctgcgcacgg ctatcccgac gccccgcgca
     1021 agggtgacgt gatccacggc ctgccggcgc tggaggagga ttgccacgtg tttcacgcgg
     1081 gcacggcact cgacggcaag gacgtgatcg tcaacggcgg gcgcgtgctt gcggtcaccg
     1141 cgctgggcgg caacgtgagc gcggcgcagc ggcgcgccta cgagatcgcc gagaagatcc
     1201 acttcgacgg aatgcagttc cggcgcgata tcggccaccg cgcgcttgcc gggggccggc
     1261 ggtagcagga gggaggaatc gaaatgaacg cgaattacga ccgcgcgcgc caggacctgg
     1321 gcaatatcgt gctgctcgag cacgtgaacg tcaccattcc cgaccagcgt ttcgccacga
     1381 ttttctacct cagcggcctg ggcctgacgc gcgacccgta tctcatgacc gggatcgaca
     1441 atatgtgggt gaacgtcggc cgccaccagt ttcatctgcc ctcgcgcggc atccaggtat
     1501 tgcgcggcca ggtcggtatc gtgatgtccg acctggacgc gctcaagcgc cgcctgcaaa
     1561 aggtggcgcc gctgctggcc gaaacgcgct tcgcctggga agagcgcgac ggcgccatcg
     1621 aaacgacgtg cccgtggggc aaccgtgacc gcggccatgc gcccgccccc gagtttggcg
     1681 ataccgagct tgcgattgcg tacgtggcct tcgacgttcc gcccggcgcc gcgcggggga
     1741 tcgcacgttt ctacgaagag atcctgggag cgcacgccgc gctggagggc gatacggcca
     1801 tcatctccgt cagcgccacc cagcggctgc gttttcgcga caccgcgcgc ccgctgccgg
     1861 aatacgacgg gcatcatgtc cagatttatg tttccgattt ttccggtccc caccgtcgcc
     1921 tgctcgagcg cggcctggtc accgaggagt cgagcgaaca ccagtaccgc ttccgcgatc
     1981 tggtcgaccc ggcgagcggg aaagcgctgt tcgccatcga gcacgaagtg cgcagcctga
     2041 cccatccgct ttatgcgcgc ccgctcgtca accgtaaccc ggcgcagacc aatcgcgatt
     2101 acacccgcgg gcaagatgcc ttccgcggca cgtactgacg cgccgccccc ttttcagtac
     2161 cctgctagaa ttcccgcatc accccgcgca gcggcggttc cggctcgcgg ggaacaagcg
     2221 aagaggaggc cccgtgccgg tcccgttccg catcgctccc aggttcctca cggcggtcat
     2281 ttcggccacc gcgctgctcg ctttttccca tgcgacaccg gcgcaggaag cgtttccggg
     2341 gaaaaggccg atcgagttcg tcgtgctgtt tccggccggc agctccgccg acgtcaccgc
     2401 ccgcgtactg gccgaaggca tgggccggga gctggggacg cagatcgtcg tggtgaaccg
     2461 gccgggcggc ggcggggcgg tcggctacaa gtacgccgcg gggcaggctg cagacggcca
     2521 tcacctggtg tggaactcca attcgatctc gaccacctat cacaccggca tgctggcttt
     2581 cgattacagg tcgttcgcgc cggtggcgcg cgtgcaggtc gagactcccc tgttgttcgt
     2641 ccggggcggg gcgccgtgga agagcctgcg cgatctcctg cagtacacga gggccaaccc
     2701 cggcaagctg aacgtcggca actccggcac gggcagtcat acccatttct cgtcggtgtg
     2761 gctgttccgg accgccggcg cgcagatcgt ggacgtcccg ttcaacgccg cgcaagtggt
     2821 gcccggtctg ttgggcggtc acgtcgaggc catcatgcaa ctgccgggcg cgctggccgg
     2881 ccacgtgcaa gccggcaccg tgcgggccct ggtcgccctg agcggagctc gcgacccgtt
     2941 tttccccgat gtgccgaccg cgcgcgagca gggcgtggat gccgtcgccg agttgtggcg
     3001 cggcgttgca gtccccaggg gcacgccgac acgcgtcgta gcaaggctgg aagatgcaat
     3061 ccgcaagacc gtgacgagcc cggaattcgc cagggccact gcaagattgc tggttacgcc
     3121 tgcctttctg ccggcggccg ggtttggcaa gctgattgcc aaggaagacg cggagatagc
     3181 ccaggcggtc aaggcgctcg gcctcaggcc gtccccgaag taacactcgc gccgccgaac
     3241 tgcccccgcc gttgaccgtg gatgtcgccg ccgtaaagga gttcctggtc gggctgcagc
     3301 agacgattgt gacccgcctc gccgaggcgg ggggtcaagc cttccgccgt gacgaatgga
     3361 tacgccccgg gggcggcggg ggtatcaccc ggatgctgga ggaaggcgcc gtattcgagc
     3421 gcg
//
LOCUS       DBNGSG010000295         1934 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1621513, whole genome shotgun sequence.
ACCESSION   DBNGSG010000295 DBNGSG010000000
VERSION     DBNGSG010000295.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1621513"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="AC158Q_07665"
     CDS             complement(<1..215)
                     /locus_tag="AC158Q_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339693.1"
                     /translation="MTVLLTILAAIAVSWVLAYHRLPAIAWTVVFAIGLGLLAVYMRW
                     PQPALVTLWVLFAVAALLFNPGPVRRAL"
     gene            426..>1934
                     /locus_tag="AC158Q_07670"
     CDS             426..>1934
                     /locus_tag="AC158Q_07670"
                     /inference="COORDINATES: protein motif:HMM:NF052741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3339694.1"
                     /translation="MAQARQRGVAFAKTTRPVIGSAVRREALFVCLDGTPGRTVAWIS
                     GPAGSGKSTLAASYVEARNLPCVWYQLDPDDADVATFFHYLSHAARRLDDGRARELTA
                     FAPQYADDVASFSRRFFRQLFMRAKAPAMLVLDNLHEVPANSPLHAAIEAGLTQVPNR
                     SCVMIISRTEPPASLARMRVGGGMVHLGWEDLRIGPQEIADIANLRGQALSPDAVAKL
                     HERTQGWAAGLVLMLEHAKVSGNIAELPGGATPKVIFDYLAGEIFDRFEPKTQDFLLR
                     IACLPRMSAEVAAALTGEARAGRLLLNLAQNDYFVREVAEDAERIYQLHPLLREFLRN
                     RAAQALPDAVSAANLARAAEILRGAGHLEDAVSLLAEGRDWAGVSRIIAEEADDMLLQ
                     GRSETLCAWLELLPSGLMEADPRLLYAHAAGRIHASPRSAQRLFAHAYESFQLAGNSR
                     GMTRSCCGVVRAIILELDDLTTLDHWIGVLAKLTAESADPAAAATLIRALLLR"
BASE COUNT          338 a          648 c          643 g          305 t
ORIGIN      
        1 agcgcacggc ggaccgggcc gggattgaac agcagcgcgg caacggcgaa caggacccac
       61 agcgtgacga gcgccggctg cggccagcgc atgtagaccg ccaggaggcc gagcccgatc
      121 gcgaacacga cggtccacgc gatggcaggc aggcggtgat aagcgagtac ccaggaaacc
      181 gcgatcgcgg ccaggatggt cagcaggacg gtcatgactg cccctttatt cgagccggtg
      241 cgacacggac agttgcgccg ccgccggttc gggccagcat acactcgcga tccggccgca
      301 gcaaccgctt caattccgta actcattgac ccattgagat tttgtggaca tgcgatgacc
      361 ccggcgggct ttgactgccc gctgaccatc atgcgaagat caactggggt gggggagaaa
      421 ccagaatggc tcaagccagg cagcgcggag ttgccttcgc gaagacgacg cggccggtca
      481 tcggcagcgc cgtgcggcgc gaggcgcttt tcgtctgcct cgacgggaca cccggccgga
      541 ccgtagcgtg gatctcgggt ccggccggtt ccggcaagag caccctggcg gcaagttacg
      601 tagaggcacg caatcttccc tgcgtctggt accagctcga cccggacgac gccgacgtcg
      661 ccacgttttt ccactacttg agccacgccg cgcgcaggct ggacgatggc cgcgcgcgcg
      721 agctcacggc attcgcaccg cagtacgccg acgatgtcgc gtcgttctcg cgcaggttct
      781 tccgccagct gttcatgcgc gccaaggcgc ccgccatgct ggtgctcgat aacctgcacg
      841 aggtgccggc caacagcccg ctgcacgccg caatcgaagc aggcctcacc caggtgccga
      901 atcgttcctg tgtgatgatc atcagccgca ccgagccgcc agcgtccctg gcgcggatgc
      961 gcgtaggcgg cgggatggtg cacctcggct gggaagacct gcgcatcggg ccgcaggaga
     1021 tcgccgacat cgccaatctg cgcggccagg ctctttcgcc ggatgcggtg gcgaagctcc
     1081 acgagcgcac gcagggctgg gcggccggac tggtgctgat gctcgaacac gcgaaggtct
     1141 ccggcaacat cgccgagctg ccgggaggcg cgacgccgaa ggtgatattc gattaccttg
     1201 cgggcgaaat cttcgaccga ttcgagccca aaacacagga tttcctgctc aggatcgcct
     1261 gcctgccgcg gatgagcgcg gaagtggccg cggccctgac cggtgaagcg agggccgggc
     1321 gcctcctgct caatctggcg cagaacgatt acttcgtgcg ggaagttgcg gaggatgcgg
     1381 agcgcattta tcaacttcat ccgctgctgc gcgaatttct gcgcaaccgc gctgcccagg
     1441 cgcttcccga cgcggtgagc gccgcgaacc tcgcgcgcgc agcggagata ttgcgcggtg
     1501 ccggtcatct cgaggacgcc gtatcactgc tcgcagaagg ccgcgactgg gcgggggtct
     1561 cccgcatcat tgctgaagag gcggacgata tgctgctgca gggccgcagc gagactctct
     1621 gcgcctggct ggaactgctg ccctccgggt tgatggaagc cgacccgcgg ctgctctacg
     1681 cgcatgccgc cggccgcata cacgcgagcc ccagatcggc gcaacggctg ttcgcgcacg
     1741 cctacgaaag tttccagctc gccggcaact cgcgcggcat gacgcggagt tgctgcggcg
     1801 tggtgcgcgc gatcatcctg gagctcgacg acctcacgac cctcgatcat tggatcggcg
     1861 ttcttgccaa gctcaccgcc gagagcgccg atccggctgc cgccgcgacg ctcatccgcg
     1921 cgctgctcct gcgc
//
LOCUS       DBNGSG010000296        15482 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1113041, whole genome shotgun sequence.
ACCESSION   DBNGSG010000296 DBNGSG010000000
VERSION     DBNGSG010000296.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 15482)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15482)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15482
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1113041"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="AC158Q_07675"
     CDS             <1..349
                     /locus_tag="AC158Q_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339695.1"
                     /translation="FALYNAVCDVVTGADGTLERMMKGLVGILVAGVLLVQPLADARD
                     RRTGHSPVLQVQGQPAQKKGPGSFQRGERDKRVERDKRVERDKGRQNRLTQEERRELN
                     RDLDRANREIYRR"
     gene            610..1746
                     /locus_tag="AC158Q_07680"
     CDS             610..1746
                     /locus_tag="AC158Q_07680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein"
                     /protein_id="HIR3339696.1"
                     /translation="MLATRIRQKDGIFYFASIPAKDILAKVRFISRFYGEGDQIAPQT
                     ISQSDEIAQFIARIEHNDAAFQRQLSRAKVRALKNFYEMAVSQPPIPGTVLLFTSEKL
                     EFRALGSEQSVGHLQEPGSKFLIIDGQHRLAALQFYLRERPGEGNNIHVPCVIFDSRS
                     EDFAAEMFVIINSTPTRINKSHLVDLYERISWAEPDRRFAAKLVDKLYREGDSPLKYR
                     INRLGGRSQQEKWILQAELFNELHRWVSGEWRKIQRASHVTREADRYYAIVRDFLKAC
                     QKVWTTSWGHPNYMVTKPVTLKAMIRVCADLARADAEPADGRQARWEKRLAPWTERMR
                     EFRMEGFYERFPAKGQVERVARLHRDLAKMADIEVQGRQRNAGN"
     gene            complement(1853..2200)
                     /locus_tag="AC158Q_07685"
     CDS             complement(1853..2200)
                     /locus_tag="AC158Q_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012356116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR3339697.1"
                     /translation="MSIKRHNPGPVLCRAVEHGGLVYVAGTTAANRSAPCKAQTEEVL
                     KTIDAALAAAGSDKSKLLWAQVWLADIREKEQMDAAWKAWVDPDNKPVRACIEARLGT
                     PDTRVEIMVTAAK"
     gene            2306..2743
                     /locus_tag="AC158Q_07690"
     CDS             2306..2743
                     /locus_tag="AC158Q_07690"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013392.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HIR3339698.1"
                     /translation="MPKVLVIHGAGMNMRGKAQIEVFGPMKLPEYDKHIRKYASELGF
                     KVEIFHSNVEGAVINKLYASHDGGVDAAIINPAGFTTGYPALVAAINQVRFPVIELHI
                     SNPARRGRVSEVATASRGVVTGFGIFGYWVAMRGIQALLSAGK"
     gene            2787..3980
                     /locus_tag="AC158Q_07695"
     CDS             2787..3980
                     /locus_tag="AC158Q_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR3339699.1"
                     /translation="MAMNKPLPLDGIRVVEFCHVVMGPTCGLVLADMGAEVIKVEPLD
                     GDHTRKLVASGAGFFPAYNRNKKSFAVDLRSPQGREAVLKLVASADVVTENFRPGAMD
                     KLGFGEQALRKLTPALVYCSLKGFLPGPYEHRTGLDEVVQMMGGLAYMTGGRYGPLRA
                     GASVNDVMGGMFGAIAILAALHQRNRTGEGQYVQSALYENNAFLMAQHMMEGLATGKP
                     VSPMPDRVRAWALYDNFKTRDGELVFVGVVTDTQWKIFCEAFGLRELLADPSLKTNPQ
                     RVEARPRIVPIVTAIFAGMTKQELMDKCEELGLPFAPIAKPEDLFDDPHLNASGGLTP
                     VTLANGTKTRVPALPIEMDGERFGTRLDIPRVGGHTRAVLRDLGYSSEEVEGLIERRA
                     VYAAQ"
     gene            complement(4000..4317)
                     /locus_tag="AC158Q_07700"
     CDS             complement(4000..4317)
                     /locus_tag="AC158Q_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339700.1"
                     /translation="MKQVLDYLNSFVTVILILAGLGGVSYNLFKEGGWLGVILGKWWS
                     AQMAHPMIAIPVTIAVIVIGKLWIDHNKAKGHTSKVPDVMVYVIMAAGVFFIYRLIFE
                     GTF"
     gene            complement(4389..5441)
                     /locus_tag="AC158Q_07705"
     CDS             complement(4389..5441)
                     /locus_tag="AC158Q_07705"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HIR3339701.1"
                     /translation="MREYKIAAIPGDGIGVEVIAAGLKALDVLGQRDRGFKLKVEQFP
                     WSSDYYKKQGHYIPEGGLERLKQFDAIFFGAVGAPDVPDHVSLWGLRLPICQGFDQYA
                     NVRPARVLPGITSPLANGNAIDWVIIRENSEGEYSGSGGRAHRGLPEEVATETSIFTR
                     TGVERIHRFAFKLARSRPRKHLTLVTKSNAQRHGMVMWDEIFYEVAKDYPDVGTQRVL
                     VDAVTTLMVLKPQTLDTFVATNLHADILSDLAAALSGSLGIAPTANLNPERKFPSMFE
                     PIHGSAFDITGKGIANPVATFWTASMMLEHLGEKEAAARLMRAVEAVTAKRIHTPDLG
                     GEATTDIVTSAVCDSL"
     gene            5600..6277
                     /gene="tsaB"
                     /locus_tag="AC158Q_07710"
     CDS             5600..6277
                     /gene="tsaB"
                     /locus_tag="AC158Q_07710"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HIR3339702.1"
                     /translation="MKILALDTSTEYCSVALWRDGEVDAREVIAGQSHSQLLLPMVDE
                     LLDRHGLAIKSLDGIAYGRGPGSFTGLRIACGVVQGLAFGAGIGVVGVVTLAAMAQAT
                     GAGRVVCCLDARMGEVYHAAYVRAGANWDTVQAPGLYAPASAPVPPAGDWTGCGSGFA
                     AHGATLNQRYADRLSAIMPDVFPHARDIARLAAVEFKAGRAMAPEQAVPLYIRDKVAL
                     KTHERAT"
     gene            6261..6725
                     /gene="rimI"
                     /locus_tag="AC158Q_07715"
     CDS             6261..6725
                     /gene="rimI"
                     /locus_tag="AC158Q_07715"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 - protein-N-terminal-alanine
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HIR3339703.1"
                     /translation="MSARLDLNPRYRGMTAADLDAVTAIENAIYPHPWTRGNFSDSLE
                     AGYHCWMVECGGEPAGYCVVMIAAGEAHLLNLSVAAPWQRRGIGREALNFVARLARDN
                     GAGKILLEVRPSNTAAIALYTTTGFSEIAVRRDYYPAGDAREDAVVMQLVLE"
     gene            6973..7557
                     /locus_tag="AC158Q_07720"
     CDS             6973..7557
                     /locus_tag="AC158Q_07720"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR3339704.1"
                     /translation="MSLEWAQLKQRVAGCTDCPLHAKRNKTVFGVGDENADWLFVGEG
                     PGADEDAQGEPFVGQAGKLLDSMLAAIRLQRGQNVYIANVVKCRPPGNRNPEHAEALA
                     CEPYLHRQIDLIRPKLIIALGRIAAANLLASDASVASMRGKVHQYRSTPLIVTYHPAY
                     LLRSPHDKAKAWADLCFAVRTMEGMQGGADAPRS"
     gene            7732..8364
                     /locus_tag="AC158Q_07725"
     CDS             7732..8364
                     /locus_tag="AC158Q_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019255708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HIR3339705.1"
                     /translation="MFKRALSMAMLIAAALLSGCGYNTLQRNDESIKAAWAEVVNQYQ
                     RRADLIPNLVNTVKGFAGQEQAVLLGVTEARAKVGTIQATPELLNDPQAFTRFQHAQG
                     ELSSALSRLMVVVERYPELKSNQNFLDLQAQLEGTENRITVARNRYIKAVQEYNIVVR
                     SFPTNLTAMLFGHKEKPQFTVENEKAISRPPVVDFGAPRPAPASPAPAAK"
     gene            8374..9876
                     /locus_tag="AC158Q_07730"
     CDS             8374..9876
                     /locus_tag="AC158Q_07730"
                     /inference="COORDINATES: protein motif:HMM:NF014073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR3339706.1"
                     /translation="MLEAIGSGLTALLTPQAILFMFIGVVYGLVIGILPGLGGVVAMA
                     LLLPFTYGFEKAATLALLLGAHIATIWGDSVTSILFSVPGSAKGLALCFDGYPMTKQG
                     QAKRALAASATGALLGGLIGAVFLALCIPLIRPVILALGPSEYLMMALWGLTVIATFS
                     QGSLMKGLIASLLGVLTAFIGMDVVTATPRFTFGSEFLMDGISFPVAMIGLFAVSEMM
                     KLAIKGTPIVDRSLVTEVSTVWQGVCDAFRHWWLVVRSSLLGLWIGVLPGVGASVAGL
                     AAYAQAAQTSRTPERFGKGAVEGVIAPDATMGANEGGGLMPTLAFGIPGGESMALLLV
                     AFIGLGVVPGPDMLTKHLDLVFSMVWIIVIANVLVTLIGLGITQWLARLPSLQANLMV
                     PIVLSVCFVGAYATRGHIEDVLVAAVFGVLGYLMDKYHYSRANFVIGMVLAIMIERNL
                     HISLTLYGRDFLLDRPIAAAMLIFVVVTAALPFVRNWRRARKQSIAAVKT"
     gene            9873..10400
                     /locus_tag="AC158Q_07735"
     CDS             9873..10400
                     /locus_tag="AC158Q_07735"
                     /inference="COORDINATES: protein motif:HMM:NF018979.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HIR3339707.1"
                     /translation="MSRRAQENIVAAALLALFAGVIWLAQDFGPRARMIPLPLAVFGI
                     VLTVIQILWQNLRSTDELQMDLIAVRAPGALPEPDGGAGAAKGAGGKVTWRQEAGALA
                     IVAALLGLIFLAGLIPAVFLFTGGYFRVTRLYSWRASLIYTAILTAAVYLLFVEALQI
                     QPYHGLLAPLVEHFR"
     gene            10425..11444
                     /locus_tag="AC158Q_07740"
     CDS             10425..11444
                     /locus_tag="AC158Q_07740"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3339708.1"
                     /translation="MKAMRRKMLLAGALAPVVMGVPGRGRAQASDFYKDKVVNINVAG
                     GASGGHTRYARLIQPYLQKHLGAREVRITNMPGGGGLKAANYIWRAKPDGLQIFFGNV
                     STLILAPLAGSKGATFDTSKFNFLGRATSEPRVLAVGGKSAIKSIQDVQKLKRPFVYP
                     SQGTDEDFYTMAVLADAFGFKVKAVTGYEGNADTALAVIKGDGDGHITGWSESLGPVR
                     SGDKHPILMVTSQKAPEFPYIPVVTEVAPAAKRATARAMANILETHRSYIGPPGMDAQ
                     AVAAFRAGVTAALKDPALLEETKKGERPVAPMEGAVQQKVIAEITRASASLSPILTAA
                     VKEIQ"
     gene            11479..13194
                     /locus_tag="AC158Q_07745"
     CDS             11479..13194
                     /locus_tag="AC158Q_07745"
                     /inference="COORDINATES: protein motif:HMM:NF013086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR3339709.1"
                     /translation="MSTDWVSIDTDVLVVGGGVAGTMAAIPALEAGLKVVICEKGKVL
                     DHCVSIGCGVDHYLTVMDTGPEWDTPEFLLRHVPELTDGIVDMAVAGRVIREMPRIFR
                     KIESFGVDFRDRKTGDYYRLRSFGLPGTYHINFDGTDFKKHLGERVRKGKATVLMRTM
                     VVQLLVRDNRACGAIAFNFRTGEWFTIRAKAVVLAAGDVNRISQNASGLAFDSWHFPY
                     NTGDAQAMAYRAGAALANMESVEATLTPKGFSTQGLNALVSLGAYFVNKSGERFMFRY
                     DEKGENARRAVLADAVINEYLLGNGPVYLDCRHLPQDMLDRMEYTLQVDRYTLPAFYR
                     QKGVNFREELVEISVSELSIRRSGVYFRGSGLAVDTNAETSVEGLFAAGDCATVSGGI
                     SGAAVLGHIAGEGVVARVRATPGALPAIDPRAIEGVRAQAADHLRQKNGPSWRQFEDE
                     TRETVTNYVGVRRTGKGLRLALDTLCALAKREPRLAAGDLHGLMRVHEAKNIRQNAEI
                     MAVASLARHETRTGSAHWRLDYPETDDVNWRKFVLVERGPDGPAVRTLSTDRPLSDAF
                     ARAVV"
     gene            13279..13551
                     /locus_tag="AC158Q_07750"
     CDS             13279..13551
                     /locus_tag="AC158Q_07750"
                     /inference="COORDINATES: protein motif:HMM:NF026048.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3339710.1"
                     /translation="MANAKPYNPIVIDAQKCVRCDLCDWICPGDLIHHEEGNKEKLPV
                     IAYPDECWYCGLCQSICPVDAIHIVFPEQMVRNRTEVVSLLGKVVE"
     gene            13752..14600
                     /locus_tag="AC158Q_07755"
     CDS             13752..14600
                     /locus_tag="AC158Q_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3339711.1"
                     /translation="MKTAVNRVRGVERDIVQVPQPGDKAPYYEPQGNEIAVFEAAYRK
                     RLPVMLKGPTGCGKTRFLEYMAFQLKRPLVTVSCHEDLTSSDLVGRFLLEGAETVWQD
                     GPLTRAVKEGAICYLDEIVEARTDSTVVIHSLTDHRRQLTIEKKGQEIEAHEDFMLVI
                     SYNPGYQTVLKDLKPSTKQRFIALNFDFPAEETERKIILNEAPGISPELVHQLVTAGR
                     KARLLKDHGLEESASTRALVYAANMMNAGLDPVAACQVAMVNPITDDVELAEALMEIV
                     QAHFGA"
     gene            14802..>15482
                     /locus_tag="AC158Q_07760"
     CDS             14802..>15482
                     /locus_tag="AC158Q_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339712.1"
                     /translation="MGSPSSRQAAIDEQLTQLRDASLVAHRELEQVLPAIRPHGEEAA
                     LAWIIACRKLHDYDREAGRAFIRGSLEAERVSEEVLAWTGQAVQFTRWRGAWRALEGF
                     MSNLPRAYGSLGHAGQRRWAEIGFVWCGRQVDSGCAYFATPVVDLSGRQGITGIEQLT
                     QPAEELFESRKLLLGTYLGGAIRVRNLLGAQAILPWALRGADILQAARARGEAYFRLE
                     SEESLALLL"
BASE COUNT         2682 a         5006 c         5197 g         2597 t
ORIGIN      
        1 tttcgcgctt tataatgctg tttgcgatgt tgtaaccggc gctgacggga ctctggagag
       61 gatgatgaaa gggttggtcg ggattcttgt tgcaggcgtg ctgctggtgc agcctctcgc
      121 ggatgcccgc gaccggcgga ccggacacag ccccgttctg caagtccagg gccagcccgc
      181 gcagaagaag gggcccggat cgtttcagcg gggcgagcgc gacaagcggg tcgaacgcga
      241 caagcgggtc gaacgcgaca agggccgtca gaacaggctc acccaggagg aacgccgcga
      301 attgaaccgg gaccttgacc gggccaatcg cgagatttac cggcgttaga cgaggcggcc
      361 ggtcacctat cgccgcccga acctctcaac ggtgccggtt gcggcggggg attcggtgcc
      421 cggccccgat tccacgtcta tgacaccgta ttgacagccg ggcgccgctt gcaattccac
      481 atcgtgtaac cccccggggc tggttcgcgc cagcgccgct aaaacgctaa aatacggctc
      541 tcgcagccgc cctgaattgg ggcgtttctg gttcgcctgc cggggggccc ataacgggat
      601 aacaagacca tgcttgccac gcgcatcaga cagaaggacg gcatattcta tttcgcgagc
      661 atcccggcga aggacatcct cgccaaggtg cgcttcatca gccgcttcta cggggagggc
      721 gatcagatcg cgccccagac gatctcccag agcgacgaga tcgcccagtt catcgcccgc
      781 atcgagcaca acgacgcggc tttccagcgg cagttgtcgc gcgccaaggt ccgggcgctc
      841 aagaacttct acgaaatggc ggtatcccag ccgccgatcc ccggcaccgt gctgctgttc
      901 accagcgaaa agctggaatt cagggcgctg ggctcggagc agagcgtcgg ccacctgcag
      961 gagccgggct ccaagttcct catcatcgac ggccagcacc gcctggcggc gctgcagttc
     1021 tacctgcgcg agcgccccgg cgagggcaac aacattcacg tgccgtgcgt catcttcgac
     1081 agccgcagcg aggacttcgc cgcggagatg ttcgtcatca tcaactccac acccacgcgc
     1141 atcaacaaga gccacctggt ggacctctac gagcgcatct cgtgggccga gccggaccgg
     1201 cgcttcgcgg cgaagctcgt ggacaagctc taccgcgagg gcgacagccc gctcaagtac
     1261 cgcatcaacc gcctgggcgg ccgcagccag caggaaaaat ggatactgca ggcggagctt
     1321 ttcaacgaac tgcaccgctg ggtgagcggc gaatggcgca agatccagcg cgcctcccac
     1381 gtgacgcgcg aggccgaccg ctattacgca atcgtgcggg acttcctcaa ggcgtgccag
     1441 aaagtctgga ccacctcctg gggacacccg aactacatgg tcaccaagcc ggtcacgctg
     1501 aaagcgatga tccgcgtgtg cgccgatctc gcgcgtgccg acgcggagcc ggccgacggg
     1561 cgccaggcgc gctgggagaa gcggcttgcg ccgtggaccg agcgcatgcg cgagttccgg
     1621 atggaaggct tctacgagcg cttccccgcc aaggggcagg tcgagcgcgt cgcgcgcctg
     1681 caccgcgacc tggcgaaaat ggccgatatc gaggtccagg ggcggcagcg caacgccggc
     1741 aactaggccg caacagcgtg aatcgtacct ctcccaaccc tcccccttgc caagggggag
     1801 ggatagggtg ggggttcgtg agtcgtgatt cgactctctg agcggcgaag cgctacttcg
     1861 ccgctgtgac catgatctcc acccgcgtgt cgggcgtgcc gagccgggcc tcgatgcaag
     1921 cgcgcaccgg cttgttgtcc gggtccaccc acgccttcca cgccgcgtcc atctgctcct
     1981 tctcccggat gtcggccagc cagacctggg cccacagcag ttttgacttg tcgctgccgg
     2041 ctgcggcgag ggcggcgtcg attgtcttca gaacctcttc ggtctgtgcc ttgcacggcg
     2101 cgctccggtt ggcggcggtg gtgccggcga cgtagaccag cccgccgtgc tcgacggcgc
     2161 ggcacagcac ggggccgggg ttgtggcgct tgatgctcat ggggttcctc ctgggctcgg
     2221 gttggtcgaa ttttccacgg gcgaactgta tcaccaaaga tgcagtaaag tgtcggggtc
     2281 atcgaccgat caggagacgg gtgtcatgcc aaaagttcta gtcatccacg gcgccgggat
     2341 gaacatgcgc ggaaaagcgc agatcgaggt tttcggaccc atgaagctgc cggaatacga
     2401 caagcacatc cgcaagtacg cgtccgaact gggattcaag gtcgagatat tccactcgaa
     2461 cgtggaaggc gcggtcatca acaagctcta cgcgtcccac gacgggggag tggatgccgc
     2521 gatcatcaac ccggccggct ttaccacggg atatcccgcg ctggtcgcgg ccatcaacca
     2581 ggtgaggttc ccggtgatcg aactccatat ttccaatccc gcgcgccggg ggcgggtttc
     2641 ggaagtcgcg acggccagcc gcggtgtggt caccggcttc ggcatcttcg gctactgggt
     2701 cgcgatgcgc ggaatccagg ccctattgtc cgccggcaaa tagctcgcgg ccgggcgggg
     2761 gattcttgcc gggttccgga ccgtgaatgg ccatgaataa gccgctgccg ctcgacggca
     2821 tccgcgtggt cgagttctgc cacgtcgtca tggggccgac ctgcggcctg gtgctcgcgg
     2881 acatgggtgc cgaggtcatc aaggtcgagc cgctcgacgg cgaccacacg cgcaagctcg
     2941 ttgcctccgg cgcggggttc ttcccggcct acaaccgcaa caagaagagc ttcgcggtgg
     3001 atctcaggtc gccgcagggc cgggaagcgg tgctgaagct cgtcgccagc gcagacgtcg
     3061 tgaccgaaaa tttccgtccc ggcgcgatgg acaagctggg cttcggcgag caggcactga
     3121 ggaagctcac gcccgcgctc gtctactgct cgttgaaggg cttcctgccc ggcccctacg
     3181 agcaccgcac cggtctcgac gaggtagtgc agatgatggg cgggctcgcc tacatgaccg
     3241 gcgggcgcta cgggccgctg cgggccggcg cttcggtgaa cgacgtcatg ggcggcatgt
     3301 tcggtgcgat cgcgatactg gccgcgctgc accagcgcaa ccggacaggc gagggccagt
     3361 acgtgcagag cgcgctctac gagaacaacg cgtttctgat ggcgcagcac atgatggagg
     3421 gccttgcgac gggcaagccc gtatccccga tgcccgaccg cgtgcgcgcg tgggcgctct
     3481 acgacaattt caagacccgc gacggcgagc tggtgttcgt cggtgtggta accgacacgc
     3541 aatggaagat tttctgcgaa gcgttcgggc tgcgggagct cctcgccgac ccctcgctca
     3601 agaccaatcc gcaacgggtc gaagcgcgcc cgcgcatcgt ccctatcgtg accgcgatct
     3661 tcgccgggat gaccaagcag gagctgatgg acaagtgcga ggaactggga ttgccgtttg
     3721 cgccgatcgc gaagccggaa gacctgttcg acgacccgca tctcaacgcg tcgggcgggc
     3781 tcacgccggt gacgctggcg aacggcacga aaaccagggt cccggccctt cccatcgaga
     3841 tggacggcga gcgcttcgga acgcggctcg atatcccgcg ggtcggcggg cacacgcgcg
     3901 cggtgctgcg ggatctgggt tacagcagcg aggaagtgga ggggttgatc gaacggcggg
     3961 ccgtgtacgc cgcgcagtga agcggccgcc ggcaacgcgt cagaacgtgc cttcgaagat
     4021 gagccggtag atgaagaaga cgcccgccgc catgatcacg tagaccatca cgtcggggac
     4081 cttgctggtg tggcccttcg ccttgttgtg gtcgatccag agcttcccga tgacgatcac
     4141 ggcgatggtg accggtatcg cgatcatcgg gtgcgccatc tgggcgctcc accacttgcc
     4201 caggatgacg ccgagccagc cgccttcctt gaacaggttg taggacacgc cgccaaggcc
     4261 cgccagtatc aggatgacgg tgacgaacga attgaggtaa tccagaacct gtttcacgcg
     4321 gttgccccgg tagattgcgc ggaagttggc cgctattgta tcagcgggcg cacgaaaata
     4381 gtgcgggatt agaggctgtc gcagaccgcg gaagtgacga tatccgtggt ggcttcgcct
     4441 cccaggtcgg gggtatggat gcgctttgca gtcacggcct cgaccgcgcg catcaggcgc
     4501 gccgctgcct ccttctcgcc gaggtgctcg agcatcatgg acgcggtcca gaacgttgcg
     4561 accgggttgg cgatgccctt gccggtgatg tcgaacgcgg agccgtggat cggctcgaac
     4621 atggatggga atttgcgctc cgggttcagg ttcgcggtcg gggcgatgcc gaggctgccc
     4681 gagagcgcgg cggcgagatc ggacaggatg tcggcgtgca ggttggtggc gacgaaggtg
     4741 tcgagcgtct gcggcttcag caccatcagc gtcgtcaccg cgtccaccag cacccgctgg
     4801 gtcccgacgt ccgggtagtc cttcgccacc tcgtagaaga tctcgtccca catcaccatg
     4861 ccgtggcgct gcgcgttcga cttggtgacg agagtgagat gcttgcgcgg gcggctgcgc
     4921 gcgagcttga acgcgaagcg gtgtatgcgc tcgacgccgg tgcgtgtgaa gatcgaagtt
     4981 tccgtcgcca cctcttcggg caggccgcgg tgcgcgcgcc cgccgctgcc ggagtattcg
     5041 ccctccgagt tctcgcggat gatcacccag tcgatcgcgt tgccgttcgc gagcgggctc
     5101 gtgatccccg gcagcacgcg cgccgggcgc acgttggcgt actggtcgaa gccctggcag
     5161 atcggcagcc gcagccccca cagcgagaca tggtcgggca cgtcgggagc gcccaccgcg
     5221 ccgaaaaaga tggcgtcgaa ctgcttcagc cgctcgaggc cgccctcggg aatgtaatgc
     5281 ccctgcttct tgtagtagtc gctgctccac gggaactgtt cgaccttgag tttgaagccg
     5341 cggtcgcgct gccccagcac gtccagcgcc ttcaggccgg cggcgatgac ctcgacgccg
     5401 atgccgtcgc cggggatggc cgcaatcttg tattcacgca tgacttaccc tgaaaattag
     5461 ccgccaagac gccaaggcgc caagaaacac aatatcaaat cagatcgaaa cgccaggcac
     5521 gccaagagaa acaaagcggt gcgcattata ccgcccgcgc ggggaaccga cttgcgcata
     5581 ggctaaaatt cgctccagcg tgaagatcct cgccctcgat acttccaccg agtactgctc
     5641 cgtcgcgctg tggcgcgacg gcgaggtgga cgcgcgcgag gtgattgccg gacagagcca
     5701 ttcgcaactg ctcctgccga tggtggacga gttgctcgac cggcatggtt tggcgataaa
     5761 gtctctcgat ggcattgcct atggccgggg cccggggtcg ttcaccgggc tgcgcatcgc
     5821 ctgcggcgtg gtgcagggcc tggccttcgg cgccggtatc ggggtggtgg gcgtcgttac
     5881 gcttgccgcg atggcgcagg caacgggcgc agggcgcgtg gtctgctgtc tcgacgcgcg
     5941 gatgggagag gtctaccacg ctgcttacgt gagggccgga gcgaactggg atacggtgca
     6001 ggcacccggg ctttatgcgc ccgcgagcgc gccggtgccg ccggcaggcg actggaccgg
     6061 ctgcggcagc gggtttgccg cgcatggagc gacgctgaat cagcgctacg cggaccggct
     6121 ctcggctatc atgccggacg tgttcccgca cgcgcgggac atcgcgcggc tcgccgcggt
     6181 ggagttcaag gcgggcaggg cgatggcgcc cgagcaggcg gtgccgctgt acatccgtga
     6241 caaggtggcg ttgaaaaccc atgagcgcgc gacttgacct gaacccacgc taccggggca
     6301 tgaccgccgc cgacctcgat gcggtcaccg cgatcgagaa cgccatttat cctcatccct
     6361 ggacgcgcgg taatttcagc gactcgctcg aggccggcta tcactgctgg atggtggagt
     6421 gcggcggcga gcccgccggc tactgcgtgg tgatgatcgc ggccggcgag gcgcacctgc
     6481 tcaatctcag cgtggccgcg ccgtggcagc gccggggcat cgggcgcgaa gcgctcaact
     6541 tcgtcgccag gctcgcgcgc gacaacggcg ccgggaaaat cctcctcgag gtgcggccgt
     6601 cgaacaccgc ggccatcgcg ctgtatacca ccaccggatt ttccgagatc gcggtgcgcc
     6661 gcgactacta tccggccggt gacgcgcgcg aggacgcggt ggtcatgcag cttgtgctgg
     6721 aataagagtg actcgttgct cgtttctcgt tactcgtttt actcgtaaac cgtgacaaac
     6781 cgccgcgact tgatccttga ggaaatgggc ctgacgccgg tctggcgtct gcgcgagagc
     6841 gcgccggccg ccgaacaggc tccggccgcc gcacccgcgg cggttcaaga gccgcgagcc
     6901 acaagtaacg agaaacgaga aacgagaaac gagtcaccgc agcccgccac gggagatcgc
     6961 cgcggtcaga tcatgtccct tgagtgggcg cagttgaagc agcgggtggc gggctgcacg
     7021 gactgcccgc tgcacgcaaa acgcaacaag accgtgttcg gtgtcggcga cgagaacgcc
     7081 gactggctgt tcgtgggcga gggccccggc gcggacgagg acgcgcaggg cgagccattc
     7141 gtgggccagg ccggcaagct gctcgacagc atgctggcgg cgatccggct gcagcgcggc
     7201 cagaacgtgt acattgccaa cgtcgtcaag tgccggcctc ccggcaaccg caatcccgag
     7261 catgcggaag cgctggcgtg cgagccttat ctccatcgcc agatcgacct gatccggccg
     7321 aaattgatca tcgcgctggg caggatcgcg gcggccaatc tgctggcgag cgacgcgagc
     7381 gtcgccagca tgcgcggcaa ggtgcatcag taccgcagta caccgctcat cgtcacctat
     7441 cacccggctt acctgctgcg cagcccgcac gacaaggcca aggcctgggc cgatctttgt
     7501 ttcgcggtcc gcaccatgga aggcatgcag ggcggggcgg acgccccacg cagctgaact
     7561 gcgtctcaga tgaaacgtct cagatgaagc gtcccagacg aggcgtctca gatgaaacgt
     7621 atcagatgaa gcgttccgta aagcgttata gactcgctgt atcggcgacg cagtacctga
     7681 gacgtagtac ctgagacgcc ctatctgaga cgctcttgag aggaacccac tatgtttaag
     7741 cgtgctcttt cgatggcgat gttgatcgca gcggctttgc tgtccggctg cggctacaac
     7801 acgctgcagc gcaacgacga gagtatcaag gcggcatggg cggaggtcgt gaaccagtac
     7861 cagcggcgcg ccgacctgat cccgaacctg gtgaacaccg tcaaggggtt cgccgggcag
     7921 gaacaggcgg tgctgctcgg cgtcacggaa gcgcgcgcca aggtgggcac gatccaggcg
     7981 acgcccgagc tcctcaacga cccgcaggca ttcacccgct tccagcatgc ccagggcgag
     8041 ctgtcctcgg cgctgtcgcg cctgatggtc gtggtcgagc gctatcccga gctcaagtcc
     8101 aaccagaatt ttctcgacct gcaggcgcag ctcgaaggca ccgagaaccg catcacggtg
     8161 gcgcgcaatc gctatatcaa ggctgtgcag gagtacaaca tcgtcgtgcg ttcctttccc
     8221 acgaacctga ccgcgatgct cttcggccac aaggaaaaac cgcagttcac ggtcgagaac
     8281 gagaaagcga tcagccggcc gccggtggtg gatttcggcg cgcccaggcc cgcgccggcg
     8341 tcaccggctc ccgcggccaa gtaagcccgc ttcatgctgg aagccatagg cagcggcctg
     8401 accgcgctgt tgacgcccca ggccatcctg ttcatgttca tcggggtcgt ctacggcctc
     8461 gtcatcggca ttctgcccgg cctgggcggc gtcgtcgcca tggcgctgct gctgccgttc
     8521 acttacggct tcgagaaggc cgccacgctc gcgctgctgc tcggcgcgca catcgccacc
     8581 atctggggcg actcggtcac cagcatcctg ttcagcgtgc ccggctcggc caaggggctc
     8641 gcgctgtgct tcgacggtta cccgatgacc aagcagggac aggcgaaacg cgccctcgcg
     8701 gcctcggcca ccggcgcgct gctgggcggg ctgattggcg cggtgtttct cgccctgtgc
     8761 atcccgctga tccgcccggt catcctcgcg ctcgggccga gcgagtacct gatgatggcg
     8821 ctgtgggggc ttacggtcat cgcgaccttc agccagggct cgctcatgaa agggctgatc
     8881 gcgagcctgc tgggcgtgct caccgctttc atcggcatgg acgtcgtgac cgccaccccg
     8941 cgcttcacgt tcggcagcga gttcctgatg gacgggatct cgttcccggt ggcgatgatc
     9001 gggctgttcg cggtctcgga aatgatgaaa ctcgccatca agggcacgcc cattgtggac
     9061 cgctccctgg tcacggaggt gagcacggta tggcagggcg tctgcgatgc tttccggcac
     9121 tggtggctgg tggtgcgctc gagcctgctc gggctgtgga tcggcgtgct gccgggggtc
     9181 ggcgcgagcg tggccgggct cgcggcttac gcccaggcgg cgcagacgtc caggaccccg
     9241 gagcggttcg ggaaaggcgc cgtggaaggc gtgatcgcgc cggacgcgac gatgggcgcg
     9301 aatgagggcg ggggattgat gcccacgctc gccttcggca taccgggcgg cgagagtatg
     9361 gcgctgctgc tcgtcgcctt catcggcctg ggcgtcgtgc cgggacccga catgctcacc
     9421 aagcacctgg atctcgtctt cagcatggtg tggatcatcg tcatcgcgaa cgtcctggtc
     9481 accctgatcg gcctcggcat cacgcagtgg ctcgcgcgcc tgccgtcgct gcaggcgaac
     9541 ctgatggtgc cgatcgtgct gtcggtgtgc ttcgtcggcg cctatgccac ccgcggccat
     9601 atcgaggacg tgctggtcgc cgccgtgttc ggcgtgctgg gctacctcat ggacaagtac
     9661 cactactcgc gggcgaactt cgtcatcggc atggtgctcg ccatcatgat cgagcggaac
     9721 ctccatatct cgctcacgct ctacggcagg gattttctcc tcgaccggcc gatcgcggct
     9781 gccatgctga tcttcgtcgt ggtcaccgcc gcgctgccgt tcgtccgcaa ctggcgccgc
     9841 gcgcgaaaac aatccatcgc agcggtgaaa acatgagccg gcgcgcgcag gagaatatcg
     9901 tcgcggcggc gctgctcgcg ctgttcgcgg gcgtcatctg gctcgcccag gacttcggcc
     9961 cgcgcgcgcg catgatcccg ctgccgctcg ccgtcttcgg catcgtgctc accgtcatcc
    10021 agatcctgtg gcagaacctg cgctcgaccg acgagctgca gatggacctg atcgcggtcc
    10081 gggcccccgg cgcgctgccc gagccggacg ggggcgccgg ggcggcgaag ggcgccggcg
    10141 gaaaggtcac ctggcgccag gaagccggcg ccttagccat cgtcgctgcc ctgctcggcc
    10201 tgatcttcct ggcgggcctc atccccgccg tgttcctgtt caccggcggc tatttccgcg
    10261 tgacccgcct gtattcatgg cgggcgagct tgatttacac tgccatcctg accgcagcgg
    10321 tatacctgct gttcgtggag gccctgcaga ttcaacccta ccacgggctg ctcgcgccgc
    10381 tggtggagca tttcagataa accggtatcc ggaggagatc agcaatgaag gcgatgcgca
    10441 ggaaaatgct gttggcgggg gcgctcgcgc ccgtggtgat gggcgtgccg gggcgtgggc
    10501 gcgcgcaggc ttccgatttc tacaaggaca aggtcgtcaa catcaacgtg gccggcggcg
    10561 cgtccggggg gcacacgcgc tacgcgcggc tgatccagcc ttacctccag aagcacctgg
    10621 gcgcgcgcga ggtgcggatc accaacatgc cgggcggcgg gggcctcaag gccgccaact
    10681 atatctggcg ggccaagccc gacgggctcc agatcttctt cggcaacgtc tccaccctga
    10741 tcctcgcgcc gctcgcggga agcaagggcg ccacgttcga cacgtcgaag ttcaactttc
    10801 tcggccgcgc caccagcgag ccgcgggtgc tcgccgtcgg cggcaagtcc gcgatcaaat
    10861 cgatccagga cgtgcagaaa ctgaagcgtc ccttcgtcta tccctcgcag ggcaccgacg
    10921 aggatttcta caccatggcg gtgctcgccg atgccttcgg cttcaaggtg aaggcggtga
    10981 ccggctacga gggcaacgcc gacaccgcgc tcgccgtgat caagggcgac ggcgacggcc
    11041 acatcaccgg ctggagcgag tcgctggggc cggtgcgctc cggcgacaag cacccgatcc
    11101 tcatggtgac ctcgcagaaa gccccggaat tcccgtacat accggtggtc accgaggtcg
    11161 cgcccgcggc gaagcgcgcg accgcccgcg cgatggcgaa catcctcgag acgcaccgca
    11221 gctacatcgg ccccccggga atggacgccc aggcggtggc ggccttccgc gccggggtga
    11281 ccgccgcgct gaaggatccg gccctgctgg aagagacgaa aaagggcgag cggccggttg
    11341 cgccgatgga aggcgccgtg cagcagaaag tgatcgcgga gatcacccgc gccagcgcaa
    11401 gcctctcgcc catcctcacg gccgccgtga aggaaatcca gtagtgccca ctgttccgcg
    11461 cgccgggcgg cattccgttt gagcactgac tgggtctcga tcgacaccga cgtcctcgtc
    11521 gtcggcggcg gcgtcgcggg cacgatggcc gcgatcccgg cgctggaggc ggggttgaag
    11581 gtcgtgattt gcgagaaggg gaaggtgctc gaccactgcg tcagcatcgg ctgcggcgtg
    11641 gaccattacc tgacggtgat ggataccggg ccggagtggg acacgcccga gttcctcctg
    11701 cgccacgtgc cggagctgac cgacggcatc gtcgacatgg cggtcgccgg gcgcgtcatc
    11761 cgcgagatgc cgcgcatctt ccggaagatc gagtcgttcg gcgtggactt cagggaccgc
    11821 aagaccgggg attattaccg gctgcgctcg ttcggcctgc ccggcaccta ccacatcaac
    11881 ttcgacggca ccgatttcaa gaagcacctc ggcgagcgcg taagaaaagg caaggcgacg
    11941 gtgctgatgc gcaccatggt cgtgcagctg ctggtgcgcg acaaccgcgc ctgcggcgcg
    12001 attgctttca acttccgcac cggcgagtgg ttcacgatcc gggcgaaggc agtggtgctg
    12061 gccgcgggcg acgtgaaccg catctcgcag aacgcttccg gcctcgcgtt cgacagctgg
    12121 catttcccct acaacaccgg cgacgcgcag gcgatggctt accgcgcggg cgccgcgctc
    12181 gccaacatgg aatcggtgga ggcgacgctg acgcccaagg gcttctcgac gcaggggctg
    12241 aacgcgctgg tgagcctggg cgcgtacttc gtgaacaaga gcggcgagcg cttcatgttc
    12301 cgctacgacg agaagggcga gaacgcccgc cgcgcagtgc tggcggacgc cgtcatcaac
    12361 gagtacctgc tgggcaacgg gccggtctac ctcgattgcc gccatctgcc ccaggacatg
    12421 ctcgatcgca tggaatacac gctgcaggtg gaccgctaca cgctgccggc cttctacagg
    12481 cagaaaggcg tcaattttcg cgaggagctg gtcgagattt cggtctccga gctttccatc
    12541 cgccgcagcg gcgtctattt ccgcggcagc gggctggcgg tggacaccaa cgcggagacc
    12601 tcggtggagg gcctgttcgc ggcgggcgac tgcgccacgg tgagcggggg catctccggg
    12661 gcagccgtgc tcggccatat cgcgggcgag ggcgtggtcg cgcgcgtccg cgccacgccc
    12721 ggggccctgc ccgcgatcga tccgcgcgcg atcgagggcg tgcgcgccca ggctgcggac
    12781 cacctcaggc agaagaacgg cccgtcctgg cggcaattcg aggacgagac gcgcgaaacg
    12841 gtgaccaatt acgtcggcgt gcggcgcacc gggaagggcc tgcgcctcgc gctcgacacg
    12901 ctgtgcgcgc tggcgaagcg cgagccgcgg ctcgcggccg gcgacctgca cggtctcatg
    12961 cgcgtgcatg aagcgaagaa catccggcag aacgccgaga tcatggccgt ggcgtcgctc
    13021 gcccggcatg agacccgtac cggctcggcg cattggcggc tcgattaccc ggagacggac
    13081 gacgtgaact ggcgcaagtt cgtgctggtc gagcgcgggc ccgacggccc ggcggtgcgc
    13141 acgctgtcca ccgaccgccc gttgtccgac gcctttgcgc gcgcagtcgt ctgaaccccg
    13201 tatcaattag ccgccaagtc gccaagcaaa tcaatcaata ttcgaaaccc cggctcactc
    13261 cctcgacact cgaccaatat ggcgaacgcc aagccctaca acccgatcgt gatcgacgcg
    13321 cagaagtgcg tgcgctgcga cctgtgcgac tggatctgcc cgggggacct cattcaccac
    13381 gaggaaggca acaaggagaa gctgccggtg atcgcctacc cggacgagtg ctggtactgc
    13441 ggcctgtgcc agtccatctg cccggtggac gccatccaca tcgtctttcc cgagcaaatg
    13501 gtgcgcaacc ggaccgaggt ggtcagcctg ctgggcaaag tcgtcgaatg accggtgagg
    13561 cagtgacgaa gtgacgaagt gacgtagtga cggtggttcc agccctgttt gtcctgaccc
    13621 tgaatctccg atccccgcgc ctgtccaact ggtgggcgga tttcccagcc gcgcgtcgta
    13681 cgcttacaat atgattattc tgattttctt ggcggtcttg gcgtcttggc ggctaattta
    13741 acggggttta gatgaagaca gcagtaaatc gcgtacgcgg cgtcgagcgc gacatcgtcc
    13801 aggtgccgca gccgggcgac aaggcgccgt actacgagcc gcagggcaac gagatcgcgg
    13861 tgttcgaggc cgcttaccgc aagcgcctgc cggtgatgct gaaaggtccg accggctgcg
    13921 gcaagacgcg tttcctcgag tacatggcgt tccagctgaa gcgaccgctc gtcaccgtgt
    13981 cgtgccatga ggacctgacc agttccgacc tggtcgggcg cttcctgctc gaaggcgcgg
    14041 agaccgtgtg gcaggacggg ccgctcaccc gcgcggtgaa ggaaggggcg atctgctatc
    14101 tcgacgagat cgtcgaggcg cgcaccgact ctaccgtcgt gattcactcg ctcaccgacc
    14161 accggcgcca gttgacgatc gagaagaagg gccaggagat cgaggcgcac gaggatttca
    14221 tgctggtgat ctcgtacaac ccgggctacc agacggtgct gaaggatctg aagccctcga
    14281 ccaagcagcg cttcatcgcg ctcaacttcg attttcccgc cgaggaaacc gagcgcaaga
    14341 tcattctcaa cgaggcgccg ggcatctcgc cggaactcgt tcaccagctg gtcaccgccg
    14401 ggcgcaaggc gcggttgctg aaggaccacg ggctagagga gtcggcctcc acgcgcgccc
    14461 tggtgtacgc cgccaacatg atgaacgccg ggctcgaccc ggtcgcggcc tgccaggtcg
    14521 cgatggtcaa tcccatcacc gacgacgtgg agctcgccga ggcgctgatg gagatcgtcc
    14581 aggcgcactt cggcgcgtga gcaagagtat tttggccacg gaatcacacg gaaacacacg
    14641 gactcgcgga caaggactgt tgcctgaggg cgaggggcaa gaacaggggt ctgctcgtcg
    14701 gcccggcgcc gtacccggca gaagctcggc gttttgtttt gtgttggctc ccgtgtcatt
    14761 ccgcgtgttt ccgtggctga acaccgctcc tggcctttga gatgggttcc ccttcttcca
    14821 gacaagccgc gatcgacgag cagctgacgc aactgcgcga cgcgagcctc gtcgcgcacc
    14881 gcgagctgga gcaggtgctg cccgcgatcc ggccgcacgg cgaggaagcc gcgctcgcct
    14941 ggataatcgc ctgccgcaag ctccacgatt acgaccgcga ggcgggccgg gccttcatcc
    15001 gcggcagcct ggaggcggag cgggtttccg aggaagtcct ggcgtggacc gggcaggcgg
    15061 tgcaattcac ccgctggcgc ggcgcgtggc gcgcgctcga aggtttcatg tcgaacttgc
    15121 cgcgcgctta cggctcgctc ggccacgccg ggcagcggcg ctgggcggaa atcggctttg
    15181 tctggtgcgg ccgccaggtc gacagcggct gcgcctattt cgcgaccccg gtcgtggacc
    15241 tgtcgggacg ccagggcatc accggcatcg agcagctgac gcagccggcg gaagagctgt
    15301 tcgagtcgcg caagctcctg ctgggcacct acctcggcgg cgcgatccgg gtgcgcaacc
    15361 tgctgggcgc ccaggcgatc ctgccgtggg cgctgcgcgg cgccgacatc ctgcaggcgg
    15421 cgcgcgcgcg cggcgaggcg tactttcggc tcgaatccga ggagagcctg gcgctgctgc
    15481 tc
//
LOCUS       DBNGSG010000297         2317 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2811027, whole genome shotgun sequence.
ACCESSION   DBNGSG010000297 DBNGSG010000000
VERSION     DBNGSG010000297.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2811027"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..133)
                     /locus_tag="AC158Q_07765"
     CDS             complement(<1..133)
                     /locus_tag="AC158Q_07765"
                     /inference="COORDINATES: protein motif:HMM:NF024214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HIR3339713.1"
                     /translation="MTGNQKNIVAVLLAAGAGKRFGGDKLLHPLEDGVAIAAHAARNL
                     "
     gene            278..1054
                     /gene="hyi"
                     /locus_tag="AC158Q_07770"
     CDS             278..1054
                     /gene="hyi"
                     /locus_tag="AC158Q_07770"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HIR3339714.1"
                     /translation="MPKFCANLTMLWNELEFMDRFGAAAKAGFKGVEYLFPYDHDKNR
                     IAAELKKHGLVQVLHNLPAGDWAAGERGIGCHPGRTKEFQDGVAKAIEYATALGCSQV
                     NCLAGIRPRHGDPIDARETFIKNLQYAAPRLKDAGVRLLIEAINTRDIPGFFLNHTKQ
                     ALDIIQAVGSDNLFVQYDIYHMQIMEGDLARTIEANLKLIPHMQLADNPGRNEPGTGE
                     INYPFLFRHIDRLGYAGWIGCEYKPATTTDAGLGWVRPYL"
     gene            1156..2100
                     /locus_tag="AC158Q_07775"
     CDS             1156..2100
                     /locus_tag="AC158Q_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HIR3339715.1"
                     /translation="MNVTRKLSLCAIALTAAFALPSAAQELKMMTGPQGGSWYPLGGA
                     IQGLLEKNIPGVKMSVAPGAGISNVLGVQTGKADLGFGNASSTYDGAIGRDPFKQKTA
                     NVCHVATLYFQYFHMVALADAGIKNVGDATGKALTTQQKGNTGEQMTRDVLKLYGLDY
                     GKMSKVNYGSYNDSVSQLKDGHAQLFGLITTVPASAVMDLASAREIRVLEIPEDKFKA
                     AQALNRGYDKRIIKAGSYPKQDKDIQTIGTWTHLIASCKLPEQLVYNITKTLATNVND
                     LGNVVAAVKGLSVKDMATDVGVPLHPGARKYYLEAKAL"
BASE COUNT          467 a          795 c          685 g          370 t
ORIGIN      
        1 tcagattgcg cgcggcatgg gcggcgatcg ccacgccgtc ctccagcggg tgcagcagct
       61 tgtccccgcc gaaacgcttc ccggcgccgg ccgcgagcag gaccgcgaca atattcttct
      121 gattcccagt catatcgccg ccgattataa cggcaccgcc gcccgcgtcc gcgcctgaac
      181 cccctgcgcg acgagaaaac cggcgagccg ctatactgcg gctctccgcc ttgtttcagc
      241 cagccgtcac ccatcactca ccaccgaagt taacgccatg ccgaaattct gcgccaatct
      301 cacgatgctc tggaacgagc tcgaattcat ggaccgcttc ggcgccgccg cgaaagcggg
      361 attcaagggc gtcgagtacc tgttccccta cgaccacgac aagaaccgca tcgccgcgga
      421 gctgaagaag cacggactcg tgcaggtgct gcacaacctg cccgccggcg attgggcagc
      481 gggtgagcgc ggcatcggct gccacccggg ccgcacgaag gagttccagg atggcgtcgc
      541 gaaagcgatc gaatacgcca ccgcgctcgg ctgcagccag gtcaactgcc tcgccggcat
      601 acgcccgcgg cacggggatc ccattgatgc gcgggaaacc ttcatcaaga acctgcagta
      661 cgcggcgccg cggctgaagg acgccggcgt ccggctgctg atcgaggcga tcaatacccg
      721 cgatatcccg ggcttcttcc tgaaccacac gaagcaggcg ctggacatca tccaggcggt
      781 cggctcggac aacctgttcg tgcagtacga catctaccac atgcagatca tggagggcga
      841 cctcgcccgc accatcgagg ccaacctcaa gctgataccc cacatgcaac tcgccgacaa
      901 cccggggcgc aacgaacccg gcaccggaga gatcaattac ccgttcctgt tccggcacat
      961 agaccgcctg ggctatgcgg gctggatcgg ctgcgagtac aagccggcca ccaccaccga
     1021 tgccgggctg gggtgggtca ggccttacct gtaacggccg gcttgcttga cggcgggcga
     1081 acgtcgccac acaatcactt cacaatcgcg cccggccgcc gcggtgcgcc cgccattacc
     1141 cacaaggagg gaatcatgaa cgttacccgc aagttgtcgc tttgcgcgat cgccctgact
     1201 gccgcattcg cgctgccgtc tgccgcgcag gaactcaaga tgatgaccgg cccgcagggc
     1261 gggtcgtggt acccgctcgg cggcgccatc cagggcctcc tggagaagaa catccccggc
     1321 gtgaagatgt ccgttgcccc gggcgccggg atttccaacg tgctgggcgt gcagaccggc
     1381 aaggccgacc tcgggttcgg caacgccagt tccacctacg acggggcgat cggccgcgat
     1441 cccttcaagc agaagaccgc aaacgtatgc cacgtcgcca cgctctactt ccagtacttc
     1501 cacatggtcg cgctcgccga cgcgggcatc aagaacgtgg gggacgcgac cggcaaggcg
     1561 ctcacgaccc agcagaaggg aaataccggc gagcaaatga cccgcgatgt gctgaagctc
     1621 tacggcctcg actacggcaa gatgtcgaag gtgaactacg gctcctacaa cgattcggta
     1681 tcacagctga aggacggcca tgcccaactg ttcgggctga tcaccacggt gcccgcttcg
     1741 gcggtgatgg atctcgcctc ggcgcgcgaa attcgcgtgc tcgagattcc cgaggacaag
     1801 ttcaaggctg cgcaggcgct caacaggggc tacgacaagc gcattatcaa ggcgggaagc
     1861 tatccgaagc aggacaagga tatccagacc atcgggacct ggacccatct catcgcaagc
     1921 tgcaagctgc ccgagcaatt ggtctacaac atcaccaaga ccctggcgac gaacgtcaac
     1981 gacctcggca acgtggttgc cgcggtcaag ggcctgtcgg tgaaggacat ggcgaccgac
     2041 gtcggcgtgc ccttgcaccc cggcgcgcgc aagtactacc tggaagccaa ggcgctctga
     2101 tcgcctcgag caatcacagc ggcgcgggca tcgcgccgct gtttttttgt ccggctaatc
     2161 cgaccccatg acgaccttcg agcgcacgct gcggcaactg atcgccgtca tcgcgatcgg
     2221 aatgtcgctg taccatctga ccatcgcctt caacggcgcg ccgcagcagc tcttcttccg
     2281 cagcacccac ctgctgttcg cgctggagct ggtgttc
//
LOCUS       DBNGSG010000298         2226 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_773919, whole genome shotgun sequence.
ACCESSION   DBNGSG010000298 DBNGSG010000000
VERSION     DBNGSG010000298.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_773919"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..962
                     /locus_tag="AC158Q_07780"
     CDS             222..962
                     /locus_tag="AC158Q_07780"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3339716.1"
                     /translation="MTDPIIVTRGLTKEYRMDSYTVAALRGVSLEIAAGEFVAVMGPS
                     GSGKSTFMNLLGCLDSPSGGEYHLAGDRVSEQSGDALAAIRNRRIGFVFQNFNLLART
                     SAVENVELPLLYSHCPAEERRQRAMARLAQVGLADRAHHHPSQLSGGQQQRVAIARAL
                     VNNPVLILADEPTGALDTRTSFELMGLLQQLNRDGMTVVLVTHEHDIATFASRIIGFR
                     DGHVVDDQRVARPADAQALLRDFDKEAA"
     gene            1008..>2226
                     /locus_tag="AC158Q_07785"
     CDS             1008..>2226
                     /locus_tag="AC158Q_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3339717.1"
                     /translation="MTWLASIRIALRALRVNKLRSTLTMLGIIIGVAAVITMIAVGSG
                     AQARVEDQIKSLGSNLIMLLPGSVTSGGVRMGAGSRNTLTEADGYAIGREIADVQASA
                     PQLRGTGQVVAGNNNWSTVFYGVTPDYFEARNWVIATGNGFEPADLTGAAKVALLGET
                     VARNLFGESDPVGQVVRIRKVPFTVIGTLQRKGQSLMGQDQDDVILMPISTARNRVLG
                     GSTARQRTVGSIFVKIREGADMKEAENEIRSLLRQRHRLQPGADDDFTLRNLSEVLAA
                     REESSRIMTLLLAAVASVSLLVGGIGIMNIMLVSVTERTREIGLRMAVGARGRDILSQ
                     FLVEAVTLALIGGLLGIAFGVAGSYAIGHFAEWHTELDFEAIVLAVGFAAAVGVFFGF
                     YPARKASRLLPIEA"
BASE COUNT          363 a          718 c          772 g          373 t
ORIGIN      
        1 tgccgacggc acctacagcg aggtggtggg cggtgagctg aaggaaggga tgcagatcat
       61 cgtcgggacg cagacggatg cgaaatcgcg gggccaggcc aagggcggcc cgcgcttcgg
      121 cttctaacgg ccgtaaatcg taaaacgcga atcgtgaatc gcaagagcgc tgttccggtt
      181 cacgatttac gattcacgtt tcacggcaca tgggtttgaa catgactgac ccgatcatcg
      241 tcacgcgcgg tctcaccaag gagtaccgca tggacagcta caccgtcgcg gcgctgcgcg
      301 gggtgtcgct cgagattgcc gcgggggagt tcgtcgcggt gatgggtccg tcgggctccg
      361 gcaaatccac gttcatgaac ctgctcggct gcctcgattc gcccagtggc ggcgaatacc
      421 acctcgccgg ggacagggta tccgaacagt ccggtgacgc gctcgcggcg atccgcaacc
      481 ggcgcatcgg tttcgtgttc cagaatttca acctgctcgc gcgcacttcg gcggtcgaga
      541 acgtggagtt gccgctgctc tacagccatt gcccggccga ggaacggcgg cagcgcgcca
      601 tggcgcggct cgcccaggtg gggctcgccg atcgcgccca tcatcacccc tcgcagctct
      661 ccggcggcca gcagcagcgg gtcgctatcg cgcgggcgct ggtcaacaac ccggtgctga
      721 tactggccga cgagccgacc ggcgcgctcg acacgcgcac cagcttcgag ttgatgggcc
      781 tgctgcagca gctgaaccgg gacgggatga cggtggtgct ggtcacccat gaacacgata
      841 tcgccacctt cgcctcgcgc atcatcggct ttcgcgacgg gcacgtggtg gacgaccagc
      901 gcgtcgcccg gccggccgat gctcaggcgc tgctgcgcga tttcgacaag gaagcggcat
      961 gagcggcgga ggcgcagcgt gattaccgag acttctggag agttgacatg acctggctcg
     1021 cgagcatccg cattgcgctg cgcgcgctgc gcgtgaacaa gctgcgcagc acgcttacca
     1081 tgctgggcat catcatcggg gttgccgccg tgatcaccat gatcgcggtc ggctccggcg
     1141 cccaggcgcg ggtggaggac cagatcaagt cgctcggctc caacctgatc atgctgctcc
     1201 cgggctcggt gacctcgggc ggcgtgcgca tgggcgcggg ctcgcgcaac acgctgaccg
     1261 aggccgacgg ctacgcgatc gggcgcgaga ttgcggatgt gcaggcgtcc gccccgcagt
     1321 tgcgcggcac cgggcaggtg gtggcgggca acaacaactg gtccacggtg ttctacggtg
     1381 tgaccccgga ctacttcgaa gcgcgcaact gggtcatcgc caccggcaac ggcttcgagc
     1441 cggcggacct caccggcgcg gccaaggtcg cgctgctcgg cgagacggtg gcgcggaatc
     1501 tgttcggcga atccgatccg gtcgggcagg tcgtgcgcat ccgcaaggtg ccgttcacgg
     1561 tgatcggcac cctgcagcgc aagggtcaaa gcctgatggg gcaggaccag gacgacgtca
     1621 tcctgatgcc gatctccacc gcccgcaacc gcgtgctggg cggcagcacc gcgcgccagc
     1681 gcaccgtggg gtcgattttc gtcaagatcc gcgaaggcgc cgacatgaaa gaggccgaaa
     1741 acgagattcg aagcctgctg cgccagcgcc accggctgca gcccggggcg gatgacgact
     1801 tcaccctgcg caacctgtcg gaagtgctgg cggcccgcga agagtcgagc cgcatcatga
     1861 cgctgctgct cgcggcggtg gcctcggtct ccctgctggt gggcggcatc ggcatcatga
     1921 acatcatgct ggtgtcggtg accgagcgca cccgcgaaat cgggttgagg atggcggtcg
     1981 gcgcgcgcgg ccgcgacatc ctgtcgcagt tcctggtgga agccgtgacg cttgcgctca
     2041 tcggcgggct gctcggcatc gcgttcggcg tggcgggttc ctacgcgatc ggccatttcg
     2101 ccgaatggca caccgagctc gacttcgagg ccatcgtcct ggcggtgggc tttgccgccg
     2161 cggtgggcgt gttcttcggt ttttatcccg cgcgcaaggc ttcgcgcctg ctgccgatcg
     2221 aggcgc
//
LOCUS       DBNGSG010000299         6284 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3491270, whole genome shotgun sequence.
ACCESSION   DBNGSG010000299 DBNGSG010000000
VERSION     DBNGSG010000299.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6284)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6284)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6284
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3491270"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="AC158Q_07790"
     CDS             <1..632
                     /locus_tag="AC158Q_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HIR3339718.1"
                     /translation="RALADWRSLPTDLGAIFDKELSLDGGAVAPMVTWGTNPEQAGPV
                     TGMVPDLRGVSDENRRAEYESALDYMGLQSGMPLQDIRVDRVFIGSCTNSRIEDLRAA
                     AEVARLGKATIPAWVVPGSGTVKAQAEREGLDRVFIAAGFEWREPGCSLCTAINGDQL
                     KPGERCASTSNRNFRGRQGTGGRTHLVSPAMAAAAALKGRITDVRELIK"
     gene            641..1261
                     /gene="leuD"
                     /locus_tag="AC158Q_07795"
     CDS             641..1261
                     /gene="leuD"
                     /locus_tag="AC158Q_07795"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF002458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HIR3339719.1"
                     /translation="MQPFTQLTAIGAPIDEPNIDTNQLCPTRFNKVPRGPKHRDILFH
                     DRRFNADGSEKEHLLNVEPFRRAQIIVGNRNWGGGSSRESAVYALYEFGIRCVIAPSF
                     ADIHSNNCAKNGLLTVVLPDGECAVLRKQLREKPGTTLSVDLESQAVTDAQGKVYRFN
                     IHPVRKKCLLEGLDDVARTAQYDGAFAAFESDYYAERPWLRSNDSP"
     gene            1366..2376
                     /locus_tag="AC158Q_07800"
     CDS             1366..2376
                     /locus_tag="AC158Q_07800"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3339720.1"
                     /translation="MKAVFASIAVLVLSVVFPAAGNAQTSPYEGKTITIVCGFKTGGG
                     YDRTARILARHLPKYIPGNPTVIVQNMPGANSIIAANHVYGVAKPDGLTIGTFNRNLV
                     LAQLTKVSGVKFDQTKYAWIGSAASESTVLTIRNDLPYKNFDELKKAGKPIVIGATGP
                     GANTYDFPLLLKELLGVDIKIVSGYSSSSDIMLAVERKEVDGRAGSYSSLRTFIDRGL
                     VRPLVRARAIEKGIENLPVDESLATNPRAKAIMALRSAPEVVGRPYVMAPGTPDAMLK
                     IMRDAFAKALKDPELLAEAKKARMDLEFMPGDEALKIIQEVLAQPKDVVDEFGKYIKF
                     GE"
     gene            2373..3884
                     /locus_tag="AC158Q_07805"
     CDS             2373..3884
                     /locus_tag="AC158Q_07805"
                     /inference="COORDINATES: protein motif:HMM:NF014073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR3339721.1"
                     /translation="MIDAFFEGLLLVLQWKAFAFMLLGIVLGFWVGLLPGLGGTTTLA
                     LMLPFIYTMEPVEGFAFLLGMHAVVATTGDITSVLFGVPGEGTTAATILDGHAMAKNG
                     EAGRALGAVLASSLVGALVGALALALAVPVVRPLVLSFGSPELFMIAIVGLAFIASLS
                     AQGARGMLRGFLAGGLGLMVATVGQDPQAGVARYTFDSLYLWGGIDLVPVLIGIFAIP
                     EIIDLAIRGTSIAGELPPGRLNRGALEGVKDTFRHFWLTIRCSLIGTFIGIMPGLGGA
                     VAQWMAYGHAAQSARTAEERKGFGQGDVRGVLGPGAANNSKEGGALIPTVAFGVPGSS
                     SMAVLLGGFFLLGLVPGPDMLTKHLAVTFSMVWTIALANIITVVACFLFLNQLARLTA
                     VRGHLLIPVIVVLIFIGSYTANSHYGDILLMLLFGGIGYLMIVGGWPRAPFVLGLVLG
                     KIAENYLYISVARYDAAWLTRPIVLVLLALAVLVICYPLVQSRRERAKDNRHA"
     gene            3877..4353
                     /locus_tag="AC158Q_07810"
     CDS             3877..4353
                     /locus_tag="AC158Q_07810"
                     /inference="COORDINATES: protein motif:HMM:NF018979.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HIR3339722.1"
                     /translation="MHNKASLVLGVAIMISSGCAVFVASGWPWKAALFPIVIGIPVFC
                     LAAAEVLWVILGSASASPAMDLRMSSDLPARIVARRTLQAAAWMIGFFAAIALTGFPV
                     AVPLFVFLYLRLQGRESWWQSLAFSALVWGCFYGIFDRLLHLPFPAGWIQGWLGIA"
     gene            complement(4420..5322)
                     /locus_tag="AC158Q_07815"
     CDS             complement(4420..5322)
                     /locus_tag="AC158Q_07815"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3339723.1"
                     /translation="MDAPCLPPLPNPSQPELKTPALACDSHFHIFGPTSRFPFSPERA
                     YTPAEAPLEKLLALHALLGFERGVAIQGNGHGTDNSAMLDALKREPGRLRGVAIVKES
                     TTRQEVRRMADAGVRALRFHIAPRQPGRFSSIGIDAFEKLAPFMAECDMHVQFFMDAR
                     ELPGFLQRLKDWKRPIVLDHFGSTKAAEGTAAPGFDTLRKQLGEGRIWVKVSGAYRAS
                     GQYPDYPDARPLHEALLKANPDQLLWGSDWPHPRLDKDMPDTGHLLDLFNAWTPGASL
                     RRKILVDNPAKLYGFSENLISDRR"
     gene            5423..>6284
                     /locus_tag="AC158Q_07820"
     CDS             5423..>6284
                     /locus_tag="AC158Q_07820"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR3339724.1"
                     /translation="MIPAKRLRYSAIVDRPPLRLPEKARVVVWPVVNVEVWDIGRPMP
                     RQVLPPPTGVTLLPDVPHWGWHEYGNRVGFWRIKAILDELKIVPSLSVNARVCLDYPR
                     IARACRDAGWEFMGHSYDQRPTHTEPDAAEMIRRSVKVLREFTGKRPVGWMGPGLTET
                     LDSPEQLAAAGIRYVADWVIDDEPCLIRTRKGPLVTMPYTLELNDIAMMVVQHHTAKE
                     FETRCMDQFDRLYQEGRERAKVMAIAVHPYISGVPHRIRYFESVMKRLRKQKNVLIWN
                     GEQILDWYLKS"
BASE COUNT         1067 a         1950 c         2107 g         1160 t
ORIGIN      
        1 ctagggcgct tgccgactgg agatcgctgc cgaccgacct gggcgcgatc ttcgacaagg
       61 aattgtcgct ggacggcggt gccgtcgcgc cgatggtgac ctggggcacg aacccggaac
      121 aggccggccc ggtcaccggc atggtccccg acctgcgcgg cgtgtcggac gagaaccgtc
      181 gtgccgagta cgagtcggcg ctcgattaca tggggctgca atccggcatg ccgctgcagg
      241 acatcagggt ggaccgcgtg ttcatcggct cgtgcaccaa ttcgaggatc gaagatttgc
      301 gcgcggccgc cgaggtggct cgcttgggga aggcaaccat tccggcgtgg gtggttcccg
      361 gctccggcac ggtaaaagcc caggccgaga gggaaggcct cgaccgcgtt ttcattgcgg
      421 cgggtttcga atggcgcgag cccggctgct ccctgtgcac cgccatcaac ggcgaccagc
      481 tgaaacccgg tgagcgctgc gcgtcgacgt cgaaccgcaa cttccgcggc cgccagggca
      541 ccggcggcag gacgcacctg gtcagtccgg cgatggcggc cgccgccgcg ctgaagggac
      601 gcattaccga cgtgcgtgaa ctgatcaagt aaaggacacc atgcaaccgt ttacccagct
      661 caccgccatt ggcgcgccga tcgacgagcc caatatcgac accaatcagc tttgcccgac
      721 gcgctttaac aaggtgccgc gcgggccgaa gcaccgcgac atcctgttcc acgatcgccg
      781 cttcaacgcg gatggctcgg agaaggagca tttgctgaac gtcgagccat ttaggcgcgc
      841 gcagatcatc gtcggcaatc gtaactgggg cggcggctcg tcgcgggaga gcgcagtcta
      901 tgcgctgtac gagttcggca ttcgctgtgt cattgccccc agctttgcag acatccattc
      961 caataattgc gccaaaaacg ggctgctgac cgtcgtgctg ccggacgggg aatgcgcggt
     1021 gctgcgcaag caactgcgcg aaaagccggg taccaccctt tcggtggacc tggagtcgca
     1081 ggccgtgacg gacgcgcagg gaaaggtgta ccgtttcaac atccacccgg tgcgcaagaa
     1141 atgcctgctt gaagggctgg acgacgtcgc ccgcaccgcg cagtatgacg gtgcctttgc
     1201 agcattcgaa tcagactatt acgcggagcg cccgtggctg cgcagcaacg acagtccgta
     1261 agcaccgccg gcgctgggcg gcaggctctc tgatatcatg aagtcccgat cgcgcggcgc
     1321 cctcgggggc ggccggtaat cggcaaaatc agaacaggag ggggtatgaa agcagtattc
     1381 gcatccatcg ctgtgctggt gttgagtgtg gtcttcccgg ccgccgggaa cgcccagacg
     1441 tcgccgtacg aaggcaagac catcaccatc gtttgcggtt tcaagaccgg cggcggctac
     1501 gaccgcaccg cccgcattct ggcgaggcac ctgccgaaat acattcccgg caatcccacc
     1561 gtcatcgtgc agaacatgcc cggtgccaac agcatcatcg cggccaacca tgtctacggt
     1621 gtcgccaagc ccgacgggct gacgatcggg acgttcaacc gcaacctggt gctcgcgcag
     1681 ttgacgaaag tgagcggagt gaaattcgat caaaccaagt acgcctggat cgggtcggcg
     1741 gcgagcgaat ccaccgtact gaccatccgc aacgacctgc cgtacaagaa cttcgacgag
     1801 cttaaaaagg ccggcaagcc catcgtgatc ggcgcgaccg ggccgggagc caacacgtat
     1861 gactttcccc tcctgttgaa ggagctgctc ggcgtcgata tcaagatcgt gagtggctat
     1921 tccagcagct cggacatcat gctggccgtc gagcgcaagg aggtggacgg gcgcgccggg
     1981 tcgtattcct cgctgcggac ctttatcgac cggggcctcg ttcgcccgct ggtccgggcg
     2041 cgcgctatcg agaagggcat cgagaatctc ccggtggacg agagcctggc gacgaatccc
     2101 cgggcgaagg ccatcatggc gctgcgctcc gcgcccgaag tcgtcgggcg cccgtacgtg
     2161 atggcccctg gcacgcccga cgcgatgctg aaaatcatgc gcgatgcgtt cgccaaggcc
     2221 ctcaaggatc ccgagctgct cgcggaggcg aagaaggcaa ggatggacct cgagttcatg
     2281 ccgggtgacg aagcgctcaa gatcatccag gaagtcctgg cgcaaccgaa ggacgtcgtc
     2341 gacgagttcg gcaagtacat caagttcggc gagtgatcga cgcttttttc gagggcttgc
     2401 tgctcgtcct gcagtggaag gcgttcgcct tcatgctgct gggcatcgtg ctcggcttct
     2461 gggtcggctt gctgcccggt ctgggcggca cgacgaccct ggcgctgatg ctcccgttca
     2521 tctatacgat ggagccggtc gagggcttcg cgttcctcct ggggatgcac gccgtcgtgg
     2581 cgacgaccgg agacatcacg tcggtgctct tcggcgtgcc cggcgaaggg accacggcgg
     2641 cgacgatact cgatggccac gccatggcga agaacggcga ggccgggcgc gcgctggggg
     2701 cggtcctcgc cagctccctg gtcggcgcgc tggtcggcgc gctcgcgctc gctctcgcgg
     2761 tccccgtggt gcgcccgctg gtgctctcct tcggctcgcc cgaactgttc atgatcgcca
     2821 tcgtcggact cgcgttcatt gcctcgttga gcgcgcaagg cgcgcgcggc atgctgcggg
     2881 gctttctcgc gggcgggctg ggcctgatgg tcgccacggt cggccaggac ccgcaggccg
     2941 gggtcgcgcg ctacaccttc gattcgctgt atctctgggg cggtatcgac ctcgtgccgg
     3001 ttctcatcgg catcttcgcg attcccgaga tcattgacct cgcgattcgc gggacgtcca
     3061 tcgccggcga attgcccccg ggccgcctca accggggtgc cctcgagggc gtcaaggata
     3121 cctttcgcca tttctggctc acgatccgct gcagcctgat cggcaccttc atcggcatca
     3181 tgccggggct cgggggcgcc gtcgcccaat ggatggcgta cggccatgcc gcgcaaagcg
     3241 cgcgaaccgc ggaagagcgc aagggcttcg ggcaaggtga cgtgcggggc gtcctgggcc
     3301 ccggggcggc caacaattcg aaggagggcg gcgccctcat tcccacggtg gcgttcggcg
     3361 tgccgggcag ttcgtccatg gcggtgttgc tgggcggttt cttcctgctg gggctggtgc
     3421 cggggccgga catgctgacg aagcacctgg cggtcacttt ttcgatggtc tggacgatcg
     3481 ccctggccaa tatcatcacg gtcgtggcgt gcttcctgtt cctgaaccag ctcgccaggc
     3541 tgaccgcggt gcgcggccac ctgctgatcc cggtgatcgt cgtgctgatc ttcatcggga
     3601 gctacacggc gaacagccat tacggagaca ttctcctgat gctgcttttc ggtggaatag
     3661 gttatctcat gatcgtcggc ggctggccgc gggcgccgtt cgtgctcggc ctcgtgctgg
     3721 gcaagatcgc ggaaaactat ctttatatct cggtggcccg ctacgacgcg gcctggctga
     3781 cgcggcccat cgtgctggtc ctgctcgcgc tggcggtcct ggtgatctgc tatccgttgg
     3841 tgcagtcgcg ccgcgagcgc gcgaaggaca atcggcatgc ataacaaggc atcgctggtg
     3901 ctcggtgtcg cgatcatgat cagctcgggc tgtgccgtct tcgtcgccag cggctggccg
     3961 tggaaggccg cgctatttcc gatcgtgatc gggatacccg tgttctgcct cgccgcggcg
     4021 gaggttctgt gggtgatcct cgggtccgca tccgccagcc cggcgatgga cctgcggatg
     4081 tccagcgacc tgccggcgcg gatcgtagcc cggagaacgc tgcaggcggc cgcttggatg
     4141 atcggctttt ttgcggctat cgcgctgacc ggcttcccgg tcgccgttcc gctgttcgtc
     4201 tttctctacc tcaggctgca gggcagggaa tcgtggtggc aatcgctcgc cttcagtgcc
     4261 ctggtatggg ggtgttttta tgggatcttc gatcggctgc tccacctgcc tttcccggcg
     4321 ggctggatac agggctggct gggcattgcc tgaccgcaaa ggcgagacaa gcggcgggtg
     4381 attggtgatg aggggccccg gctgcgtcgg gatcgagcac tatcgtctat cacttatcaa
     4441 gttttcggaa aatccgtaga gcttcgcagg attgtcgacc agaattttcc ggcgcaacga
     4501 tgcgcctggc gtccaggcat tgaacaggtc gagcaggtgc ccggtatcgg gcatgtcctt
     4561 gtcgagacgc ggatgcggcc agtcgctgcc ccacaacaat tggtcggggt tggccttaag
     4621 cagcgcctcg tgcaacggcc gggcatcggg gtaatcggga tattgcccgg aggcccggta
     4681 cgccccggat accttcaccc agatgcggcc ttcgccaagc tgcttcctga gggtgtcgaa
     4741 gccgggtgcg gcagttccct ccgccgcctt ggtcgagccg aagtggtcca gcacgatcgg
     4801 acgcttccag tccttaagcc gctgcaggaa gccgggaagt tcgcgcgcgt ccatgaagaa
     4861 ctgcacgtgc atgtcgcatt cggccatgaa cggggcgagc ttttcgaatg cgtcgatccc
     4921 gatgctgctg aagcgtcccg gctggcgcgg cgcgatatga aagcgcagcg cacgcacgcc
     4981 ggcgtcggcc atgcgccgga cttcttgccg cgtggtggat tccttcacga tcgccacgcc
     5041 acgcaggcgc ccgggttcgc gcttgagcgc gtcgagcatg gcggagttgt cggtgccgtg
     5101 cccattgccc tggatcgcga cgccgcgctc gaacccgagc agcgcgtgca gcgcgagcag
     5161 tttttccagc ggggcttcgg cgggcgtgta cgcgcgctcg ggagagaatg gaaaccggct
     5221 ggtcggcccg aagatgtgga aatgcgagtc gcaagccaga gctggcgtct tcagctcagg
     5281 ctgactggga tttggcagcg ggggaaggca gggagcatcc attatttttc ccgtttttcc
     5341 ccgatgacta tatcagaccg ctaggggcac ccaagtcgct ataatggcgg gtaaagcccc
     5401 gctcgtcggg aaaaataaat ccatgattcc agcaaagcgc ctgcgctatt ccgccatcgt
     5461 cgatcgtccg ccgctcaggc ttccggagaa ggcgcgtgtc gtcgtgtggc cggtcgtcaa
     5521 cgtcgaggtc tgggacatcg ggcgcccgat gccgcggcag gtgctgcccc cgccgaccgg
     5581 cgtcacgctg ctgcccgatg tgccgcactg gggctggcac gagtacggca acagggtcgg
     5641 gttctggcgc atcaaggcga tactggacga gctgaagatc gtaccgtcgt tgtcggtcaa
     5701 cgcgcgggtg tgtctcgact atccgcgcat cgcccgggcg tgccgcgatg ccggatggga
     5761 attcatgggg cattcctacg accagcggcc gacgcacacc gagcccgacg cggcagaaat
     5821 gatccgccgc tcggtcaagg tgctgaggga attcaccggc aagcggccgg tgggctggat
     5881 ggggccgggg ctcaccgaga cgctcgacag cccggagcag ctcgccgccg cgggcatccg
     5941 ctacgtcgcc gattgggtga tcgacgacga gccgtgcctg atcaggaccc gcaagggacc
     6001 gctggtgacc atgccctaca cgctggaatt gaacgacatc gcgatgatgg tggtgcagca
     6061 ccatacggcg aaagagtttg agacgcgctg catggaccag ttcgaccggc tgtaccagga
     6121 gggcagggag cgcgcgaagg tgatggccat cgccgtgcac ccctatattt caggcgtgcc
     6181 gcaccgcatc cggtattttg aatccgtgat gaagcggctc aggaagcaga agaacgtgct
     6241 gatctggaac ggggagcaga tcctcgactg gtatctcaag agca
//
LOCUS       DBNGSG010000300         3823 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4340479, whole genome shotgun sequence.
ACCESSION   DBNGSG010000300 DBNGSG010000000
VERSION     DBNGSG010000300.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3823)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4340479"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /gene="rlmH"
                     /locus_tag="AC158Q_07825"
     CDS             <1..337
                     /gene="rlmH"
                     /locus_tag="AC158Q_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HIR3339725.1"
                     /translation="VGAVERVLESEGKRIRAALPAGCVKVVLDERGTLMRTMELARRL
                     GRWRESGRDVAFIVGGADGLDAGVKQSADFAWSLSPLTLPHGLARVLLAEQLYRAHSI
                     LHNHPYHRE"
     gene            435..1055
                     /locus_tag="AC158Q_07830"
     CDS             435..1055
                     /locus_tag="AC158Q_07830"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HIR3339726.1"
                     /translation="MSISDKRIYLASRSPRRRDLLKQIGVPFELLLLREDLRRGADVD
                     ETPLADESPGVYVLRIARTKVEMAVRQIAYRLLPQKPVLAADTTVIFDGQIVGKPDDA
                     EHAARILRALSGREHQVLTAVAVALREQVETQISVSSVWFRELTETDVRRYCASREPL
                     DKAGAYAIQGRAGAFVTRISGSYSGIMGLPLAETTELLQKFNISVW"
     gene            1074..2540
                     /gene="rng"
                     /locus_tag="AC158Q_07835"
     CDS             1074..2540
                     /gene="rng"
                     /locus_tag="AC158Q_07835"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011545847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HIR3339727.1"
                     /translation="MTEEILVNVTPQETRVAVIEQGVTQEVHIERASSRGLVGNITMG
                     RAVRVLPGMQSAFIDIGGERAAFLHVADMRGHRHNSGHNGEEPVPIEKLLGEGQPLMV
                     QVVKDPIGTKGARLSTQISIAGRFLVYLPQGSHIGISQRIEDEEERAHLREKLQLLLP
                     PEEKGGFIIRTVAETATDRELKSDIDYLRRLWAGIQDKAATAAPLTSLYQDLGLSLRV
                     LRDFVHEDTARVLVDSRETWQRMLEFGQDFTPAAAERVEHYTGERPLFDLYGVEDEIE
                     KALARRVDLKSGGYLVIDQTEALTTIDVNTGGFVGVRSFDDTIFKTNLEAAQVIARQL
                     RLRNIGGIIIVDFIDMDTEEHRNAVLNEFRKALARDRTHMSVNGFTQLGLVEMTRKRT
                     RESLAQILCEPCPACDGRARLKTAQTVCYVILRELTREARQFSAREFRILASQPVIDL
                     FLDEESQALAMLSDFIGKPVSLQVETAYNQEQYDIILM"
     gene            complement(2565..2870)
                     /locus_tag="AC158Q_07840"
     CDS             complement(2565..2870)
                     /locus_tag="AC158Q_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008540292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HIR3339728.1"
                     /translation="MNRDSTWCRYDAAAQKLTLSLHVQPGARKSAFAGIHGDALKIRI
                     AAPAVDNKANAALVGFLSETLGVPKSVIAVRRGATGRRKIIEITGGPELVGRVANLT"
     gene            complement(2897..3475)
                     /locus_tag="AC158Q_07845"
     CDS             complement(2897..3475)
                     /locus_tag="AC158Q_07845"
                     /inference="COORDINATES: protein motif:HMM:NF014389.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HIR3339729.1"
                     /translation="MLSGALIFLIETAFGLFIVALLLRFYLQWARAPHRNPLSEFLQA
                     LTDFMVKPARRFIPGLWGLDLATLALAWLIQLLELFIVLQLRGYQAGAGAGMVFATLV
                     LLALVTLARLGLYIVLAAVIIQVVLSWLSPSSPAMPLLNSLTRPFLRVFQRRIPPVGN
                     VDLSPLFLLVVIQLLLMLPVAWAEISLSRLLQ"
BASE COUNT          751 a         1196 c         1255 g          621 t
ORIGIN      
        1 cgtcggagcg gtcgagcgcg tgctcgagtc cgaagggaaa cgcatacgtg ccgcgctgcc
       61 cgcgggctgc gtgaaagtgg tgctcgacga gcgcggcacc ctgatgcgca cgatggagct
      121 cgcgcggcgc ctcgggcgct ggcgggaatc gggacgggac gtcgccttca tcgtgggcgg
      181 cgccgacgga ctcgacgcgg gcgtgaagca atccgccgat ttcgcatggt cgctgtcccc
      241 gctgacgctc ccccacgggc tcgcgcgggt attgctcgcc gagcagcttt atcgcgcgca
      301 ctcgatactg cacaatcacc cctatcatag agaataagga ttcaggatcc gggattcggt
      361 attcagagag cgacgcggga aagtgattat ccgaacgccg aatcctgatt cctgaatcct
      421 gaatcctgct ttcaatgagc atcagcgaca agcgcatcta tctcgcctcc cgcagcccgc
      481 ggcggcgcga tctgctgaag cagatcggcg tgccgttcga gctgctgctg ctgcgcgagg
      541 acctgcggcg cggcgcggac gtcgacgaga cgccgctggc ggacgagtcg cccggcgtct
      601 atgtcctgcg catcgccagg accaaggtgg agatggcggt gcgccagatc gcgtaccggc
      661 tgctgccgca gaagccggtg cttgccgccg ataccacggt cattttcgac ggccagatcg
      721 tcggcaagcc ggacgacgcg gagcacgcgg ctcgcatcct gcgcgcgctc tccggccgcg
      781 agcaccaggt gttgacggcg gtggcggtcg cgctgagaga gcaggtcgag acgcaaatct
      841 cggtctcgag cgtatggttc cgtgaattga ccgaaaccga cgtccggcgc tactgcgcga
      901 gcagggaacc gctcgacaag gccggcgcct acgccatcca gggacgggct ggcgcgttcg
      961 tgactcgaat ctcgggcagc tattccggta tcatgggcct gccgctggcg gaaacgacgg
     1021 aactgctgca gaagttcaat atctcggtct ggtaaggact tcatcccatg gccgtgaccg
     1081 aggaaatact ggtcaacgtc accccgcagg agacgcgggt agccgtgatc gagcagggcg
     1141 tcacgcagga agtgcacatc gagcgcgcgt cctcgcgcgg gctggtcgga aacatcacca
     1201 tgggacgcgc ggtccgcgtg ctgccgggca tgcagtcggc gttcatcgac atcggcggcg
     1261 agcgcgccgc gttcctgcac gtcgcggaca tgcgcggcca ccggcacaac agcggccaca
     1321 acggcgagga gccggttccc atcgagaaac tgctcggcga gggccagccg ctgatggtgc
     1381 aggtggtgaa agatcccatc ggcaccaagg gcgcgcggct gtcgacgcag atttcgattg
     1441 ccggccgctt tctggtctac ctgccgcagg gatcgcatat cggcatctcc cagcgcatcg
     1501 aggacgagga ggaacgggcg cacctgcgcg agaagctgca gctgctgctg ccgccggagg
     1561 agaagggcgg gttcattatc cgtaccgtgg cggaaaccgc caccgaccgc gagttgaagt
     1621 ccgacatcga ttacctgcgc cggctctggg ccggcatcca ggacaaggcg gcgaccgccg
     1681 cgccccttac ctcgctgtac caggacctgg gcctctccct gcgggtgctg cgcgacttcg
     1741 ttcacgaaga caccgcgcgc gtgctggtgg actcgcgcga gacttggcag aggatgctcg
     1801 aattcgggca ggacttcacc cccgccgccg ccgagcgcgt cgagcactac accggcgagc
     1861 ggccgctgtt cgatctctac ggtgtcgagg acgagatcga gaaagcgctg gcgcggcgcg
     1921 tggacctgaa atcgggcggc tacctcgtca tcgaccagac cgaggcgctc accaccatcg
     1981 atgtcaacac cggcggcttc gtcggcgtgc gcagcttcga cgacaccatt ttcaagacca
     2041 acctcgaggc cgcgcaggtc atcgcccgcc agctgcggct gcggaacatc ggcggcatca
     2101 tcatcgtcga tttcatcgat atggacaccg aggagcaccg aaacgcggta ctcaatgaat
     2161 tccgcaaggc gcttgcgcgc gaccgcaccc acatgtcggt gaacggcttc acccagcttg
     2221 ggctcgtcga gatgacgcgc aagcgcacgc gcgagtcgct cgcgcagatc ctgtgcgagc
     2281 cgtgcccggc gtgcgatggt cgcgcgcgcc tgaagactgc gcagacggtg tgctacgtga
     2341 tcctcaggga attgacgcgt gaagcgcggc agttcagcgc acgcgagttc cgcatcctcg
     2401 cctcccagcc ggtgatcgat cttttcctcg acgaggaatc gcaggcgctt gccatgctct
     2461 ccgacttcat cggcaagccg gtgtcgctgc aggtcgagac cgcgtacaac caggagcagt
     2521 acgacatcat cctgatgtga cgagtgacga gggacgcgcc cgcatcacgt cagattcgcg
     2581 actctcccga ccagttccgg gccgccggtg atttcgatga tcttgcggcg gccggttgcg
     2641 ccgcggcgga cggcaatcac ggacttcggg acgccgaggg tttcgctcag aaacccgacc
     2701 agcgcggcat tcgccttatt gtccaccgcg ggcgcggcga tcctgatttt cagcgcatcg
     2761 ccgtgaatgc ccgcgaatgc gctttttctt gccccgggct gcacgtggag actcaatgtg
     2821 agcttttgcg ccgcggcgtc gtaacggcac caggtcgaat cacgattcac gaatcacggc
     2881 ccatgcggat tcggcctcac tgaagcagcc gcgagagaga gatctccgcc cacgccaccg
     2941 ggagcatcag cagcagctgg atcaccacga gcaggaacag gggcgaaagg tcgacgttgc
     3001 cgaccggcgg aatgcggcgc tggaacacgc gcaggaaggg gcgcgtcagg ctgttcagga
     3061 gcggcatcgc cgggctggaa gggctcagcc aggacagcac gacctggatg atcacggcgg
     3121 cgaggacgat gtagaggccg agcctggcga gcgtgaccag cgcgagcagg accagcgtgg
     3181 cgaacaccat gccggcaccc gctccggcct ggtaaccgcg gagctggagc acgatgaaga
     3241 gctccagcag ctggatgagc cacgccagcg cgagcgtcgc gaggtcgagg ccccacaggc
     3301 cgggaatgaa acggcgggca ggcttcacca tgaaatcggt cagcgcctgc aggaactccg
     3361 agagcggatt gcggtgcgga gcgcgcgccc actgcaggta gaagcgcagc agcagggcca
     3421 cgatgaacag cccgaaagcg gtctcgatca ggaagatcag cgcgccggac agcaagcgtc
     3481 aactccaaaa tgagccgccg gtgaaagtcg atgaacgccg atgaagttca aaaaatcgac
     3541 aggatttaca ggatttccca ggatcaatga gattaagacg ggcatttcaa aaaagtccgg
     3601 cttcgaagtt ccatcctgaa aataatgttg aatcctgttt attctgatct tgattctatc
     3661 ggcgtccatc ggcgttcatc ggcggtttcg atacttagag tccctaacat aatgaaacgc
     3721 gcgctgcgct gtcacgattt tggctcaggg cgcgaagcga gccgcagcca atatgtcgat
     3781 attggcaagg cgagcgacaa agccatgggc caaaactcgt gcc
//
LOCUS       DBNGSG010000301         4800 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_605809, whole genome shotgun sequence.
ACCESSION   DBNGSG010000301 DBNGSG010000000
VERSION     DBNGSG010000301.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4800)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_605809"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            306..1535
                     /locus_tag="AC158Q_07850"
     CDS             306..1535
                     /locus_tag="AC158Q_07850"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045240 - alpha-ketoacid dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HIR3339730.1"
                     /translation="MPQLGETVAEGTVTRWYKKVGDTVAADDVLFDVETDKVSTEVPA
                     QANGVLTEILVAEGVTVKVGAKLAVIRETAPQQASRNSTASAAAPIKKAENESIRTAP
                     TAAHAIKAVAGERLSPVVRRLLAGHTLDPSAIAGTGREGRITREDVLAHIEKLGTTAR
                     TEERPPYEVGGAGTETIPLNNVRRRTAEHMAKSWATVPHVLQVVEADFFRVDQARKAA
                     GAQWKAREGWPLTYLPFIAHAVVAALAKFPRLNASFNGDHLAVHRRINLGIAVDLNFD
                     GLLVPVLKDAAGKSVAQLARAMNDLAARARAGKLKPDDMTEGTYTITNNGAFGTLITA
                     PIISPPQVAILSTDAIRKRPVVIEGPEGDTITIRSVGMLAQTFDHRAVDGSYSGAFLN
                     HVKTLIETTDWQKHLHN"
     gene            1699..2628
                     /locus_tag="AC158Q_07855"
     CDS             1699..2628
                     /locus_tag="AC158Q_07855"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3339731.1"
                     /translation="MANMKAHKLGWIGIGRMGYAMAERLAKAGCDITVWNRTRSKAEP
                     LAKSGAKIAGSLAELAACDIVFTMVSTGKDVKEVLYGPAGVTSQGKAPKIVVDSTSIS
                     LEESAEIREKLAKLGVKLLAAPVSGNAKVIKAGKLTVVASGPKDAFDAVAPYLQAVGP
                     GVSYVGEGELSRIAKICHNVMLGVVIQNLCEITLLAEKAGMKRHAFLDFLNKSVMGST
                     FTRYKAPALVNLDFHVTFTPELLRKDIDLGLAAGRAFGVPMPLTSLARDTVQTLIGNG
                     YTDQDFSTLLLLQAQASGVKLEPEQVEVADGLS"
     gene            2655..3617
                     /locus_tag="AC158Q_07860"
     CDS             2655..3617
                     /locus_tag="AC158Q_07860"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3339732.1"
                     /translation="MSKLISVILLAFIAAPLTGLAQNYPTKPVRTIIAFPPGSATDII
                     GRVIVGKLTEMWGQQVLADNRGGAGGSLGTSIAARGAPDGYTLLINSGAFTVAPAMYI
                     KLPYNVQKDFVDIAALVAMPNVLSVSPSSRLKSVGGFIAEAKANPGKINFAFAGLGSG
                     THLNNEKFKLAAKIDFTNVAYKGSGEVFPDLLAGRVDAYFAPLSAGLPYIQSGKLRPL
                     GVTSQKRASQLPNVPTLAESGVPNFEFLLWFGLWGPTGIPQPVLNKIRAGFAQALADP
                     GVKEKLGTLGTDVMNMTPAQFSKFVKSELAVSAQLFKAAGIQPQ"
     gene            complement(3699..>4800)
                     /locus_tag="AC158Q_07865"
     CDS             complement(3699..>4800)
                     /locus_tag="AC158Q_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339733.1"
                     /translation="PARAAAARPVAIHLAMARPSLSRLLRPLLWLVLLVLLSVVIPAL
                     LIITAPIFLLVWSLGFAMAVFSSSFRKEMDVTRLGVALWLPFWIFVIVGSAVPAVAAA
                     HAVVLWLDGQTPSLLPTYLDMLVRQLPRDARELLAELNVSRRDWGWFCIISGVTGVSS
                     NLVYAFIDIPWRLKQIRLLRALPRSKARSAAIGLAEFEGTVRATSADGFVTKSLTEPA
                     RPFHLEDETGRILVDPRGATVRPRSGSGVSLQVNEIEEGLREGDRVYVLGNVQPRADA
                     PPGALGPDALVVRPLDQTLVSSPVARLLFAKGRAMADRDTPNIFIVDRGREHDVALRL
                     RMTLWDFCAFGAVYVASSLWLVQAAWPWISPR"
BASE COUNT          913 a         1536 c         1605 g          746 t
ORIGIN      
        1 tgggacctcg acgggccgat cctgcgcatt accacgccgc acatcccgct gccgtcggcg
       61 gatgcgctgg agtacgcgac gattccgtcg gtggagcgca tcgtgcgcga agtgcgtgac
      121 aagatagact gaaccgccaa gaccgccaag ataaatacac agaaacaatt ttcgatcagg
      181 gcgcccggca ctttccgtat tatctgagtg caaggacgag gcgtgagtac cgatttccga
      241 tctgagtttt tcttggcggt cttggcggtt aataaggctt tcggagtttc tgaatggatg
      301 taatcatgcc gcagctcggc gagacggtcg ccgagggcac ggtcaccagg tggtacaaga
      361 aggtcggcga cacggttgcc gccgacgacg tgctgttcga cgtcgagacc gacaaggtca
      421 gcaccgaggt tccggcgcaa gcgaacggcg tgctcaccga gatcttggtc gccgagggcg
      481 tcacggtgaa ggtcggcgcg aagctcgcag tgatccgcga aacggcgccg caacaggcgt
      541 cccgcaattc cacggcttcg gcggcggcgc cgataaagaa ggcagaaaac gaatcgatac
      601 ggacagcgcc gacggccgct catgcgatca aggcagtcgc cggcgagcgc ctgtcgccgg
      661 tggtgcgccg gttgctggcc ggacacacac tggacccctc ggccatcgcg ggcaccggcc
      721 gcgaaggccg catcacgcgg gaagacgtgc tcgcccacat cgaaaagctc ggcaccaccg
      781 cccggacgga agagcgcccg ccttacgagg tgggcggcgc cggcacggag accatcccgc
      841 tcaacaatgt gcgcaggcgc accgccgagc acatggcgaa atcgtgggcc acggtcccgc
      901 acgtgctcca ggtcgtggag gcggattttt tccgggtgga ccaggcgcgc aaggccgcgg
      961 gcgcgcagtg gaaggcgcgc gagggctggc cgctcactta cctgccgttc atcgcgcacg
     1021 cggtcgtggc cgcgctcgcg aaattcccgc gcctgaacgc cagcttcaac ggcgatcacc
     1081 tcgcggtgca ccggcgcatc aatctcggca tcgcggtgga cctcaacttc gacgggctgc
     1141 tggtcccggt gctgaaggac gcggcgggca agtccgtggc gcaacttgcg cgcgcaatga
     1201 acgatctcgc cgcgcgcgcc cgggcgggta agctcaagcc cgacgacatg acggaaggca
     1261 cctacacgat caccaacaac ggcgccttcg gcacgctgat caccgcgccc atcatcagcc
     1321 cgccccaggt cgcgatcctg tccaccgacg ccatccgcaa gcgcccggtc gtgatcgagg
     1381 ggcccgaggg cgacaccatc acgatccggt cggtcggcat gctcgcgcag accttcgacc
     1441 accgcgcggt cgacggctcc tactccggcg ccttcctgaa tcacgtgaaa acgctcatcg
     1501 agacgacaga ctggcaaaag cacctgcaca attgaaccgc caagaccgcc aagataaata
     1561 cacggaaaca attttcggtt ggggcatccg gcactttccg tattgcctgg gtgcaaggac
     1621 gaggcgtgaa taccgatttt cgatctgctt ttttcttggc ggtcttggcg gttaattgat
     1681 ctttttgaag gataagcaat ggcgaacatg aaagcgcata aattggggtg gatcggcatc
     1741 ggccgcatgg gatacgcgat ggcggagcgc ctcgcgaagg cgggctgtga catcacggtg
     1801 tggaaccgca cccggtcgaa agcagagccg ctggcgaaat ccggcgcgaa gatcgcgggc
     1861 tcgctcgccg agctggccgc gtgcgacatc gtgttcacga tggtgtcgac cggcaaggac
     1921 gtgaaggaag tgctgtacgg tcccgccggc gtgacgtcgc agggcaaggc gccgaagatc
     1981 gtggtggaca gcacttcgat ctcgctcgag gagtcggcgg agatcaggga gaagctcgcc
     2041 aagctgggcg tgaagctcct cgccgcaccg gtctccggca acgccaaggt gatcaaggcc
     2101 ggcaagctga cggtcgtcgc ctcggggccg aaggacgctt tcgacgccgt cgcgccatac
     2161 ctgcaggcgg tgggcccggg cgtctcctac gtcggcgagg gcgagctctc gcgcattgcc
     2221 aagatctgcc acaacgtgat gctgggcgtg gtgatccaga acttgtgcga gatcacgctg
     2281 ctcgcggaga aggcggggat gaaacgccac gccttcctcg actttctcaa caagagcgtg
     2341 atgggctcga ccttcacgcg ctacaaggcg ccggcgctgg tgaacctcga cttccatgtc
     2401 accttcaccc ccgagctgct gcgcaaggac atcgacctgg ggctcgcggc ggggcgggcg
     2461 ttcggggtgc cgatgccgtt gacatcgctt gcccgggaca cggttcaaac cttgatcggc
     2521 aacggctata ctgaccagga cttttccacg ctgctgctgc tgcaggcgca ggcctcgggc
     2581 gtcaagctcg agccggaaca ggtggaagtc gcggacgggc tgtcttgatg attcaaccca
     2641 gcggaggagt gttcatgagc aagcttataa gtgtcatcct gttggcattc atcgccgcgc
     2701 ccctgacggg cctggcgcag aactacccga ctaagccggt ccgcaccatc atcgcgttcc
     2761 cgccgggcag tgcgaccgac atcatcggcc gcgtgatcgt gggcaagctc acggaaatgt
     2821 ggggccagca ggtactggcg gacaaccgcg gcggcgcggg cgggtcgctc ggcacctcga
     2881 tcgcggcgcg gggagccccg gacggctaca cgctgctcat caattcgggc gcgtttacgg
     2941 tcgccccggc catgtacatc aagctgccct acaacgtgca gaaggatttc gtggacatcg
     3001 ccgccctggt cgcgatgccc aacgtgctga gcgtcagccc gagctcgagg ctgaagtcgg
     3061 tcggcggctt cattgccgag gccaaggcca accccggcaa gatcaatttc gcgttcgcgg
     3121 gactgggcag cggcacgcat ctcaataacg agaaattcaa gctcgcggcc aagatcgatt
     3181 tcacgaacgt ggcctacaag ggctcgggcg aagtgttccc cgacctgctg gcggggcgcg
     3241 tcgatgccta tttcgcgccg ctctcggccg gcctgccgta catccagtcg ggcaagctga
     3301 ggccgctggg cgtgacgtcg cagaagcgcg cgagccagct gccgaacgtg ccgaccctcg
     3361 cggaatccgg ggtgcccaac ttcgagttcc tgctgtggtt cgggctgtgg gggccgacgg
     3421 gaattccgca gccggtgctg aacaagatcc gcgcgggctt tgcgcaggcg ctggccgacc
     3481 ccggcgtgaa ggagaagctc ggcacgctcg gcaccgacgt catgaacatg accccggcgc
     3541 agttcagcaa gttcgtgaaa agcgagctcg cggtcagcgc gcagctgttc aaggcggcgg
     3601 ggatccagcc gcagtgaagc caaaggcgtg aatcgtgatt cgtgaatggt gaatagcgaa
     3661 aggccgcctg agggcggcct ttttctttcg ggcgggcgct agcgcgggga gatccagggc
     3721 cacgccgcct gtaccagcca gagcgaggac gccacgtaga ccgcgccgaa ggcgcagaaa
     3781 tcccacaagg tcatgcgcag gcgcagcgcg acgtcgtgct cgcgcccgcg gtcgacgatg
     3841 aaaatgttcg gcgtatcgcg gtccgccatc gccctgcctt tggcaaacag cagccgcgcg
     3901 accgggctcg acaccagcgt ctggtcgaga ggccggacga ccagggcgtc cggcccgagc
     3961 gccccgggcg gcgcgtccgc gcgcggctgc acgttgccga gcacgtagac gcgatcgcct
     4021 tcgcgcaagc cttcctcgat ctcgttgacc tgcagggaca cgccggagcc cgaccgtggg
     4081 cgcacggtgg cgccccgggg gtccaccaaa atgcgcccgg tctcgtcctc gaggtggaac
     4141 ggccgcgccg gttccgtcag tgatttcgtc acgaatccgt ccgcgctcgt ggcgcggacg
     4201 gtgccctcga actcagcgag cccgatggcc gcggagcgcg ccttcgatcg cggcagcgcc
     4261 cgcagcaggc ggatctgctt caggcgccac gggatatcga tgaacgcgta caccaggttg
     4321 ctgctgacgc ccgtcacgcc ggagatgatg cagaaccagc cccagtcccg ccgcgacacg
     4381 ttgagttcgg ccaggagttc gcgtgcatcc cgcgggagtt gcctgacgag catatcgagg
     4441 taggtcggca gcagcgacgg cgtctgcccg tccagccaga gcacgaccgc gtgcgcggcg
     4501 gccacggccg ggacggcgga accgacgatc acgaagatcc aaaaaggcag ccagagcgcc
     4561 acccccagcc gggtgacgtc catctccttg cgaaaagagc tggagaaaac ggccatcgcg
     4621 aaaccgaggg accagaccag caggaagatc ggtgccgtga tgatcagcag tgccgggatc
     4681 actaccgaca gcagcaccaa cagtaccagc cacagcagtg gtcgcagcag ccggctgaga
     4741 gacgggcgtg ccatggccaa gtgtatcgct accgggcgag ccgcggctgc gcgggccggc
//
LOCUS       DBNGSG010000302         5900 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3153428, whole genome shotgun sequence.
ACCESSION   DBNGSG010000302 DBNGSG010000000
VERSION     DBNGSG010000302.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5900)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5900)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5900
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3153428"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /gene="lipB"
                     /locus_tag="AC158Q_07870"
     CDS             <1..367
                     /gene="lipB"
                     /locus_tag="AC158Q_07870"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:TIGR00214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HIR3339734.1"
                     /translation="VIYLLLDLRRRAITVRPLVRMMEQAVIDLLASYGIAAEGRNDAP
                     GVYVNGAKIAALGLRIRNGCCYHGLAFNVDMDLSPFRVIDPCGYSGLAVTQARDLGID
                     DGADLLGEKLVRQLLRKLA"
     gene            382..1302
                     /gene="lipA"
                     /locus_tag="AC158Q_07875"
                     /pseudo
     CDS             382..1302
                     /gene="lipA"
                     /locus_tag="AC158Q_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
     gene            complement(1489..1998)
                     /locus_tag="AC158Q_07880"
     CDS             complement(1489..1998)
                     /locus_tag="AC158Q_07880"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008333584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HIR3339735.1"
                     /translation="MPGIRVGTALAAVAAVAVTLSLLGVFWRAVPAPQVTFVSLTGER
                     IATAGLKGRVALVSFWATDCAICVKEMPDMVRTYDRFRGRGLELIAVAMRHDPPNYVI
                     RFTEQNRLPFKVALDPVGEHARAFGDVRFTPTTFVIDKRGNIVLKITGEVNFARLNAL
                     LEEKLKEPA"
     gene            2140..2820
                     /locus_tag="AC158Q_07885"
     CDS             2140..2820
                     /locus_tag="AC158Q_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HIR3339736.1"
                     /translation="MRAILSFFALLVISGHAQASLDSISNTDAVSGLRQALNDGSLAA
                     VAKLGVQNGYFANPKVKIPLPPSLQRIESALRFAGMKKQADELVLSMNRAAEAAAPEA
                     KQLLVDAVKKMSVQDAKKILTGGDTAGTEYFKRTTQSQLSQRFLPIVKKATDRVGLAQ
                     QYNSLAGQGAQLGLVKKDQATIEQYVTQKALDGLYLMIGEQEKAFRQNPVGASSDIVR
                     KVFGAMKR"
     gene            2955..3305
                     /locus_tag="AC158Q_07890"
     CDS             2955..3305
                     /locus_tag="AC158Q_07890"
                     /inference="COORDINATES: protein motif:HMM:NF017470.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HIR3339737.1"
                     /translation="MDYKKLLFSFEGRINRKPYWMFILAAIVVNIIVAVLAAMLGDVV
                     GLILTIILAIAMIWAGLAVSVKRWHDRDKSGWWVLIGLIPVIGPIWVLVECGFLTGTA
                     GQNRFGSDPLSGSA"
     gene            complement(3321..>5900)
                     /gene="polA"
                     /locus_tag="AC158Q_07895"
     CDS             complement(3321..>5900)
                     /gene="polA"
                     /locus_tag="AC158Q_07895"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006761695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HIR3339738.1"
                     /translation="FDAKGRTFRDDLYADYKANRPAMPDDLVAQLGPLKETIAAMGWP
                     LLEIKGVEADDVIGTLATQAERQGLRVIIASGDKDLAQLVNSVITMENADPRTGGREI
                     LDEAGVAKKFGVPPERIIDYLTLIGDSVDNVPGVADVGPVTAVKWLAEYGTLDNLVAH
                     AAEIEGKRGENLRKALPWLPQARALLTIKSDVELPVKAQDLALRPPDTAKLAELFERL
                     DFKSWRKELEGAPVENEQARQPAAPEVIPVPAARVHPPAPDRNYETLLTEKQLDAWIA
                     RIDKAELVAFDTETTSLDPVRAQLVGMSFSVEAGAAAYLPLAHRYTGAPPQLELESVL
                     RRLKPWLEDARRAKLGQNAKYDTHVLANHDVRLAGVKHDTLLQSYVLESHQRHDMDSL
                     AERHLGVKTITYDEVTGKGAGRIGFEQVAVERATEYAAEDADVTLRLHQALFPQVQSD
                     EKLARIYREIEMPLVAVLFGMERNGVLLDTGLLRDLSREFGEKMGKIEKKAFDEAGQP
                     FNLNSPKQIQEILFEKQGLKPVRKTPSGQPSTDEESLAELALNHPLPKLLLEYRGLAK
                     LKSTYTDTLPEMINPKTGRVHTTYGQAVAVTGRLASNDPNLQNIPIRAAEGRRIREAF
                     IAAPGCQIVSADYSQIELRIMAHISQDASLLAAFAAGEDIHRATAAEIFSLAPKDVDA
                     EQRRYAKVINFGLIYGMSAFGLTRQLGIERAAAQQYMDRYFARYPGVAEYMKRTREAA
                     RVAGFVETVFGRRLWLPEVKSGNPARRQGAERAAINAPMQGTAADLIKMAMIAADRWL
                     REQRFESRLIMQVHDELVLEVPERELGEVRRELPRLMGGVADLSVPLVVDVGAGPNWD
                     RAH"
BASE COUNT         1007 a         1913 c         1927 g         1053 t
ORIGIN      
        1 cgtgatttac ctcctgctcg acctgcggcg ccgcgcaatc acggtgcgcc cgctggtgag
       61 gatgatggag caggcggtga tagacctgct cgcctcctat ggcatcgcgg ccgaagggcg
      121 gaacgacgca ccgggcgtgt acgtcaacgg cgccaagatc gctgcgctcg ggttgcgcat
      181 ccgtaacggc tgctgctatc acggcctcgc cttcaatgtc gacatggacc tgtcgccttt
      241 ccgcgtcatc gacccctgcg gctactccgg cctcgccgtg acgcaggcgc gggatctcgg
      301 catcgatgac ggcgcggacc tgctgggcga gaaactcgtc cggcagctgc tgcggaaact
      361 cgcatgagct ccatggggga aaaacagaaa ggccggctga agacggcgcg cattcccgtc
      421 aaggtcgtgc cggcggcgcc gctcgcgaag cccgactgga tccgcgtgcg cctcggcaac
      481 ggcgggcgct tccaggaaat caagcgcatc cttcgcgagc atcgcctgca cacggtatgc
      541 gaagaggcga cctgtcccaa cattggcgag tgcttcagca agggcacggc gaccttcatg
      601 atcctcgggg acctctgcac acgccgctgc ccgttctgcg acgtcgcgca cggcaaaccg
      661 ctgtcgcccg atgccgagga gcccgccaac ctggcgcgca cgatttccga attacggctc
      721 cggtacgtgg tcatcaccag cgtggaccgc gacgatctgc gcgacggcgg cgcgcggcat
      781 ttcgccgatt gcatccgcgc ggtgcgcagc ctctcgcccc agacccggat agaagtgctg
      841 gtgccggatt tccgcggccg gctcgaggcc gcgctcgatg cgctgtcgga agcgccgccg
      901 gacgtcatga accacaacct cgaaaccgtc ccgcggctgt accggcaggc gcgtcccggc
      961 gcggattacg ccaactcgct caacctgctg aaggacttca agcagcgctt ccctgcggtt
     1021 ccgaccaagt cgggactgat ggtggggctg ggcgaaaccg acgaggaaat cctcgcggtc
     1081 atgcgcgacc tgcgcgccca cggcgtggag atgctcacca tcggccagta cctgcagccg
     1141 tcggcccatc atctgccggt ggcgcgctac gtcgtgcccg cggtgttcga cgattacgcg
     1201 cgccaggctg aggagatggg gttcgcgcac gccgcctgcg gaccgctggt gcggtcgagc
     1261 taccatgccg accggcaggc gcacgaagcc ggcgtcgcgt gaatagacag gattgtttcc
     1321 ccgctcaaaa cccgtcaaaa ccacaaatag acaggattaa caggatttac aagatttaaa
     1381 gattcacgaa acgaaaaagc cgttgtttga atcctgttaa tcttgttaat cctgtctatt
     1441 aatgacgttg acgggttttc ggtttttcga tgaagttaat cctgtcgatc aggccggctc
     1501 cttcagcttt tcttcgagca gcgcattcag cctggcgaaa ttcacttcgc cggtgatctt
     1561 gagcacgatg ttgccgcgct tgtcgatcac gaaggtcgtg ggcgtaaacc tcacgtcgcc
     1621 gaaggcgcgg gcatgctcgc ccaccggatc gagggcgacc ttgaacggca ggcggttctg
     1681 ttccgtgaag cggatcacgt agttgggcgg gtcgtgccgc atagccaccg cgatgagttc
     1741 caggccgcgc ccgcgaaacc ggtcgtaggt ccgcaccatg tccggcatct ccttgacgca
     1801 gatcgcgcaa tcggtggccc agaagctgac cagcgccacc cttcccttga ggcccgcggt
     1861 ggcgattctc tcgcccgtga gcgagacgaa agtcacctgc ggcgcgggga ctgcccgcca
     1921 gaataccccg agcagcgata gcgttaccgc gacggcggca accgcagcca gtgccgttcc
     1981 gacccgtatt cctggcatgg ctagcccgaa atcccgcccg ggcgggttcg cgcaaaaccg
     2041 gcgggattga ccacaagtag ccggaacggc actaaaatga gagtgcggat ggggcccagg
     2101 gggggccggc tggatggatg tgacaccggg ggtgcggcca tgcgtgccat tttgagtttc
     2161 tttgcgctgc ttgtcatcag cggccacgcg caagcgagcc tcgacagcat cagcaacacc
     2221 gacgcggtga gcggcctgcg gcaggcgctc aacgacggct cgctcgccgc ggtggccaag
     2281 ctcggcgtcc agaacggcta cttcgccaac cccaaggtca aaataccgct gccgccctcg
     2341 ctgcagagga tcgagagcgc attgcggttt gccggcatga aaaaacaggc cgacgagctg
     2401 gtgctgtcga tgaaccgcgc ggcggaagcg gcagcgccgg aagccaagca gttgctggtg
     2461 gacgccgtga agaagatgtc ggtgcaggac gccaagaaga tactcaccgg cggcgacacc
     2521 gcgggcaccg agtatttcaa gcgcaccacc cagtcccagc tctcccagcg cttcctgccg
     2581 atcgtcaaga aagccaccga ccgggtcggt ctcgcgcaac agtacaattc cctcgcgggc
     2641 cagggcgcgc agctcgggtt ggtcaaaaag gaccaggcga cgatcgagca gtacgtcacc
     2701 cagaaggcgc tcgacggcct ctacctcatg atcggcgagc aggaaaaggc gttccggcag
     2761 aaccccgtcg gcgcgtcgag cgacatcgtc aggaaggtat tcggcgccat gaagaggtga
     2821 cgcggacaag aagtgacgcg gacaagaagt gacgcggaca agaagtgacg cggacaagag
     2881 gtgacgcgga cgggtcccgc gtcatgcgtt tgcggcagtg accccgtacg gttcaaccaa
     2941 agggagagga aagtatggat tacaaaaaat tgctgttctc gttcgaaggc cgcatcaacc
     3001 gcaagccgta ctggatgttc atactcgcag cgatcgtcgt gaacatcatc gtggcggtgc
     3061 tcgccgcgat gctgggcgac gtggtgggtc tgatcctcac gatcatcctg gccatcgcga
     3121 tgatctgggc cggcctggcg gtgtcggtaa agcgctggca cgaccgggac aaatccgggt
     3181 ggtgggtgtt gatcggcctg atcccggtca tcggcccgat ctgggtcctg gtcgagtgcg
     3241 ggttcctgac gggaacggcg ggacagaacc gtttcgggtc cgacccgctc tcgggcagcg
     3301 cgtgacggcg tagaacggcg tcagtgggcc cggtcccagt tggggccggc gcccacgtcc
     3361 accaccagcg gcacggacag atccgcgacg ccgcccatca gccgcggcag ttcgcgcctg
     3421 acctcgccca gctcccgctc cggcacttcg agcaccagct cgtcgtgcac ctgcatgatg
     3481 agccgcgact cgaagcgttg ctcccggagc cagcggtccg ccgcgatcat cgccatcttg
     3541 atgagatcgg ccgccgtgcc ctgcatcggc gcgttgatcg cggcgcgctc cgcgccctga
     3601 cgccgggcgg gattgccgct cttcacctcc ggcagccata agcgcctgcc gaacaccgtc
     3661 tcgacgaaac cggccacgcg cgccgcctcg cgcgtgcgtt tcatgtactc cgccaccccc
     3721 gggtagcgcg cgaaatagcg gtccatgtac tgctgggcgg cagcccgctc gatcccgagc
     3781 tggcgcgtga gcccgaacgc cgacatgccg tagatcagcc cgaagttgat gaccttggcg
     3841 tagcgccgct gctcggcgtc cacatccttc ggggcgaggc tgaagatctc ggcggcggtg
     3901 gcgcgatgga tgtcctcgcc ggcggcgaaa gccgcgagca ggctcgcgtc ctgcgagatg
     3961 tgcgccatga tccgcaattc gatctgggaa tagtcggctg acacgatctg acaacccggc
     4021 gccgcgatga acgcttcccg gatgcggcgg ccttcggcag cgcggatcgg gatgttctga
     4081 aggttggggt cgttgctcgc gagccggccg gtcaccgcca ccgcctgccc gtacgtggta
     4141 tgcacgcggc ccgtcttcgg gttgatcatt tccggcagcg tgtccgtgta agtggatttg
     4201 agcttggcga gtcctcggta ctccaggagc agcttcggca gcgggtgatt gagcgcgagc
     4261 tcggcgagcg actcctcgtc ggtcgacggc tggccggagg gagtcttcct gaccggtttc
     4321 aggccctgtt tttcaaacag gatttcctgg atctgcttcg gcgaattgag gttgaacggc
     4381 tgccccgcct catcaaatgc tttcttttcg atcttcccca tcttttcgcc gaactcgcgt
     4441 gacaggtccc tcagcaatcc ggtgtcgagc agcacgccgt tgcgctccat gccgaagagc
     4501 accgcgacca gcggcatctc gatttcccga tagatgcggg cgagcttctc gtcggattgg
     4561 acctgcggga acagcgcctg gtgcagccgc agcgtcacgt ccgcgtcctc ggccgcgtac
     4621 tcggtggcgc gctccaccgc cacctgctcg aagccgatgc gccccgcgcc cttgccggtg
     4681 acctcgtcgt aggtaatggt cttcacgccg agatggcgct cggccaggct gtccatgtcg
     4741 tggcgctggt ggctttccag cacgtaagac tgcagcagcg tgtcgtgctt gacgccggca
     4801 agacggacat cgtggttcgc cagcacgtgc gtgtcgtatt tcgcgttctg gccgagcttg
     4861 gcgcggcgcg catcttcgag ccagggcttg aggcgcctca acaccgactc gagttcgagc
     4921 tgcggtggcg cgccggtgta gcggtgcgcg agcggcaggt aggcggcagc cccggcctcg
     4981 accgagaacg acatgcccac cagttgcgcc cggacgggat cgaggctggt ggtctcggtg
     5041 tcgaacgcaa caagttcggc cttatcgatc cgggcgatcc atgcgtccag ctgcttctcc
     5101 gtgagcaggg tttcgtagtt tcgatccgga gcaggcgggt gaacgcgcgc tgcggggacc
     5161 gggatcacct cgggggcggc gggttgccgc gcctgctcgt tttcgaccgg cgcgccctcc
     5221 agttcctttc tccacgactt gaagtcgagg cgttcgaaca gctcggcgag ctttgccgtg
     5281 tcgggcgggc gcaaggcgag gtcctgcgct ttcaccggca gctcgacgtc cgacttgatg
     5341 gtaagcagcg cgcgcgcctg gggcagccac ggcagcgcct tgcgcaggtt ctcgccccgc
     5401 tttccctcga tctcggccgc gtgcgccacc aggttatcga gggtgccgta ttcggcgagc
     5461 catttcacgg cggtcaccgg gccgacgtcg gccacgcccg gcacgttgtc cacgctgtcg
     5521 ccgatcaagg tcaggtagtc gatgatgcgc tcgggcggga cgccgaattt ttttgccacg
     5581 ccggcctcgt cgagaatctc gcgcccgccg gtcctggggt cggcattctc catcgtgatg
     5641 acggagttca cgagctgcgc gaggtccttg tcgccgctgg cgatgatgac gcgcaggccc
     5701 tggcgctcgg cctgcgtggc taacgtgccg attacgtcgt ccgcctccac ccccttgatc
     5761 tcgagcagcg gccagcccat cgcggcaatg gtttccttga ggggcccgag ctgcgccacg
     5821 agatcgtccg gcatcgccgg gcggttcgcc ttgtaatcgg catagaggtc gtcgcggaaa
     5881 gtcctgccct tcgcgtcgaa
//
LOCUS       DBNGSG010000303         2731 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4511574, whole genome shotgun sequence.
ACCESSION   DBNGSG010000303 DBNGSG010000000
VERSION     DBNGSG010000303.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4511574"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..806
                     /locus_tag="AC158Q_07900"
     CDS             81..806
                     /locus_tag="AC158Q_07900"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3339739.1"
                     /translation="MPEARQETLRLDDVRKSYGIGTPVQTEVLHGIDLVLERGEFAAL
                     IGPSGSGKSTLLNVIGLLDHPTAGRVFIERQEAGTLGDDELTRLRGRSIGFVFQHHYL
                     LPEFTALENVMMPILAARGRPDEDMRGVAATLLDRVGLTPWRDHKATNISGGQQQRVA
                     IARSLAMKPALVLADEPTGNLDTRSADSVFDVLRDINKSSDTTFLIVTHDPRLAQRCD
                     RIVELVDGRIVSDKANHEVVRRG"
     gene            1025..2236
                     /locus_tag="AC158Q_07905"
     CDS             1025..2236
                     /locus_tag="AC158Q_07905"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008342762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3339740.1"
                     /translation="MFLEDRLERVIDRIRGNAAIPLRLELWDGRRFDFSSNPTVTVAI
                     PRRSALQYFIPPDLNKLGEAFVEGYIRVEGPIHEVFRAAEGLARAAARTRAGIGRVAH
                     HDREQDRKAIEYHYDVSNDFYSLFLDRELVYSCAYYRSEDDSLEMAQAQKLDHILNKL
                     ALKPGERFLDIGCGWGALIRRAAKHYGAVSTGITLSKNQYEYARERIRSEGLEDRCTV
                     ELRDYRDVPGTALFDKIASVGMFEHVGLKNLAGYFSKIHSLLKEGGLVLNHGITTSDV
                     DNGWMGLGAGEFIDRYVFPDGELPHIALVLKEMSAAGLEVADVESLRRHYARTCHEWA
                     SRLEANARQAERTAGEKRVRTWEIYLAGCAFGFARGWMNVYQVLAFKAAPDRVSPLPL
                     TRDYMYPPFRP"
     gene            2521..>2731
                     /locus_tag="AC158Q_07910"
     CDS             2521..>2731
                     /locus_tag="AC158Q_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339741.1"
                     /translation="MTIGAICDRRIATASRTTSVFVAAKSMCAFDERVLVVTDRKDGR
                     LSAVGIVTEHEFVSRIAALRADPAAL"
BASE COUNT          486 a          857 c          913 g          475 t
ORIGIN      
        1 ataaccatcg ccccctcacc ccttccccct ctcccgaggg gagaggggaa acggatcgcg
       61 gcaagcggtc cacaaaccac ttgccggaag ctcggcagga aacgctgcgc ctcgacgacg
      121 tgcgcaagtc ctacggcatc ggcacgccgg tgcagacgga ggtgctgcac ggaatcgacc
      181 tggtgctcga gcgcggggag ttcgccgcgc tcatcgggcc gtccggctcg ggcaagagca
      241 cgctgctcaa cgtgatcggg ctgctggatc atccgacggc cggccgggtg ttcatcgaga
      301 gacaggaggc gggcacgctg ggcgatgacg agctgacgcg gctgcgcggc cgttcgatcg
      361 gcttcgtgtt ccagcaccac tacctgctgc cggaattcac cgcgctcgag aacgtcatga
      421 tgccgatact ggcggcgcgc ggccggcccg acgaggacat gcggggcgtg gcggcgacac
      481 tgctcgaccg cgtcggcctg acgccctggc gggaccacaa ggcgaccaac atttcaggcg
      541 ggcagcagca acgggtcgcc attgcccggt cgctcgccat gaagccggcg ctggtgctgg
      601 ccgacgagcc caccggcaac ctggacacca ggtcggccga cagcgtgttc gacgtgctgc
      661 gcgacatcaa taagtccagc gacacgactt tcctgatcgt gacccatgac ccgcggctcg
      721 cgcagcgttg cgaccgcatc gtcgagctgg tggatggccg catcgtctcc gacaaggcca
      781 accacgaagt cgtgcggcgg ggatagcgcc gccgcgcctg cccgtcacac ggtcccgacg
      841 cctggcacgc cattgccatg caacgcccgg gcactccggg cgaccggggt ggtcttatga
      901 caggggctcc tggtccccct gcccgggaac ccggcaggtt ggcgacccgg attgacgcaa
      961 atcaacttgc cacgggcgga agggtgtaat ttaatcgatt ctcgggggca tcgggagcgt
     1021 agccatgttc cttgaagacc gactcgagcg ggtcatcgat cgcatccgcg gcaacgcggc
     1081 cattccgttg cgcctggaat tatgggatgg acggcggttc gatttctcgt ccaatcccac
     1141 cgtaacggtg gcgattccca ggcgttcggc actgcagtat ttcatccctc cggatctcaa
     1201 caagctcggc gaggcgttcg tcgagggata catccgcgtc gaagggccga tccacgaggt
     1261 cttcagggcc gcggaaggcc tggctcgcgc cgccgccagg actcgcgctg gaatcggccg
     1321 tgttgcgcat cacgaccgtg aacaggaccg caaggcgatc gaataccact acgatgtctc
     1381 gaatgatttt tactcccttt ttctcgaccg cgagctggtc tattcctgcg cctactaccg
     1441 gagcgaggac gactccctcg agatggccca ggcgcagaag ctcgaccaca tactgaacaa
     1501 gcttgcgctg aagcccgggg agcggtttct tgacatcggg tgcggctggg gcgcgctcat
     1561 ccggcgggcg gcgaagcact acggcgccgt ttcgaccggc atcacgctgt cgaaaaacca
     1621 gtacgagtat gcgcgagaac ggatccggtc cgagggcctg gaggaccggt gcacggtgga
     1681 gttgcgcgat taccgcgatg tgcccggcac cgcgctgttc gacaagatcg ccagcgtcgg
     1741 catgttcgag cacgtcggcc tgaagaatct ggcaggttat ttttcgaaga tccattcgct
     1801 tctgaaggag ggggggctcg tgttgaatca cggcatcacg accagcgacg tggacaatgg
     1861 ctggatgggt ctcggagcgg gggagttcat cgatcgttac gtattcccgg atggcgagct
     1921 gccgcacatt gcgctcgtcc tcaaggaaat gtcggcggcc ggactggagg ttgcggacgt
     1981 cgaatcgttg cggcgccact acgcgcgcac ctgccacgaa tgggcgagcc ggctcgaggc
     2041 caacgccaga caggcggagc gcaccgcggg agaaaagcgg gttcgtacct gggagatcta
     2101 cctggcgggg tgtgccttcg gatttgcccg gggctggatg aacgtctacc aggtgcttgc
     2161 gttcaaggcc gcgcccgatc gggtcagtcc gctgccgctc acgcgggatt acatgtatcc
     2221 gccgttccgt ccttagtcgt tcgacccggg cggagtcggg aggcttgatt tggatcaggc
     2281 cgtgcccgtt gcgacctgac tattctcgaa tcgccatccg ggatgccggg cgagtcccgg
     2341 ttgccagggc gggggttcga gacattccat ggctgcatca atccgccccc gccgcccggc
     2401 gcggtcgtcg ccgacctcgg ccatcgccat cgccgcgatt gatccaaatc aatgacgttt
     2461 tttccacatc gataagaatg gagcaagcaa attccggcag acgtggacgg gaggtgtgac
     2521 gtgacgatag gggcgatatg cgaccggcgc attgcaacgg cctcgaggac caccagcgtt
     2581 ttcgtggcgg cgaaatccat gtgcgcgttc gacgagcgcg tcctggtggt caccgacagg
     2641 aaggatggac ggctgtccgc ggtggggatc gtgacggagc acgagttcgt gtcccggatc
     2701 gccgcgctgc gggccgaccc cgccgcgctc a
//
LOCUS       DBNGSG010000304         6518 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4512346, whole genome shotgun sequence.
ACCESSION   DBNGSG010000304 DBNGSG010000000
VERSION     DBNGSG010000304.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6518)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6518)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6518
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4512346"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            276..785
                     /gene="pyrR"
                     /locus_tag="AC158Q_07915"
     CDS             276..785
                     /gene="pyrR"
                     /locus_tag="AC158Q_07915"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HIR3339742.1"
                     /translation="MPGNPLPDAEQLLKALIEQMRPAIGPETGLIGIHTGGVWVAERL
                     HQALKLQTPLATLDVSFYRDDYQARGLHRDVKRSEIPFEVEGRDLILVDDVLYTGRTI
                     RAALNEIFDYGRPTAVRLATLVDRGGRQLPIAAQFVGATLALTPGRSVGLKRDARGRL
                     ELEMRAEKS"
     gene            785..1738
                     /locus_tag="AC158Q_07920"
     CDS             785..1738
                     /locus_tag="AC158Q_07920"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014897897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HIR3339743.1"
                     /translation="MWLHPSNPQLDKKGQLHHLLTLDGLPAEILWQILDTAKSFVGTR
                     VKKVPLLRGKSVFNVFFEPSTRTRTTFEIAAKRLSADVINLNVTVSSQSKGESLLDTV
                     DNLSAMHADMFVVRHGESGAPHFIARHVAPDIHVINAGDGRHSHPTQGLLDMYTIRHY
                     KKDFSNLRVAIVGDVLHSRVARSQIHALTTLGCPEIRVIGPKTLLPAHVEQLGVHVYH
                     DMRQGLKDVDVVTMLRLQNERMQGALLPSAQEFYKFYGLTDDKLAVARPDAIVLHPGP
                     MNRGVEIDSSVADGKQSVILPQVTFGIAIRMAVMSIMAGNK"
     gene            1835..3112
                     /locus_tag="AC158Q_07925"
     CDS             1835..3112
                     /locus_tag="AC158Q_07925"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008646280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIR3339744.1"
                     /translation="MKIHIKGGRLIDPKSGVDAKKDVYITAGRIVSIGDAPNGYTANR
                     VIDAAGLVVCPGLVDLAARLREPGFEYMATLDSEMEAAIAGGVTSLACPPDTDPPLDE
                     PGLVEMLKFRAKNLNQARVYPVGALTQGLKGARLTEMVELVDAGCVAVSQADAPFADT
                     QVFYHALQYAATFGFPVWLRPQDPALAQHGVAHDGPVATRLGLPAIPVCAETVALSTI
                     LILMRATGARVHLARLSSAEGVEMVRQAKHEKLPISCDVAIHHAHLTEMDIGYFDSNS
                     RLDPPLRSQRDRDALRAALADGTVDALCSDHTPVDADVKQLPFAEAEAGATGLELLLP
                     LTLKWAEESKVPLATALARITSEAARVLNVDAGHLTPGHAADICIFDPGRHWKVEPAA
                     LKSQGKNTPFLGLELKGRARYTLVDGQVVYETK"
     gene            3221..5266
                     /locus_tag="AC158Q_07930"
     CDS             3221..5266
                     /locus_tag="AC158Q_07930"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012213068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HIR3339745.1"
                     /translation="MNPLLDFSGLPRFADFNPGLITPAVETLLAENRALIERVVDPGV
                     PASWRDFVQPLEDANERLRRAWGVAGHLNAVMNSPELREAYNANLPKVTQYYTELGQH
                     EGLYAKFKALGRGPGFEGLTRAQKKIVENELRDFRLGGAELPPGKKARFKEVSEKLSQ
                     LSSRFSDNLLDATNAFAHDITDPAEVSGIPEDVLAAARAAAQADGKTGWKFTLHAPSY
                     LPVMQYADRRSLRELMYRAYVTRASEFGKPEWDNTSLIAGILQLRQELARLIGFASYA
                     EYSLEPKMAESPQQVLDFLGELAARAKPYAERDLKEVTEFARAELGLPKLESWDFAYA
                     SEKLRAKRYAFSDQEVKQYFPETRVLPGMFKVVETLYGLTIAPADAPVWHPDVRFYAV
                     RDRAGGLVGQFYVDLYARPSKRGGAWMDDAVARRLKDGRVQAPVAYLNCNFSAPVGGK
                     PALFTHDEVNTLFHEFGHGLHHLLTRVDYLGVSGINGVEWDAVELPSQFMENFCWEWG
                     VLAPMTAHVDTGAPLPRALFDKMLAARNFQSGMQMVRQLEFALFDLRLHHDYDPADTK
                     TGAKTVLELLDEVRRRVAVVFPPDYNRFPHNFSHIFAGGYGAGYYSYKWAEVLSADAY
                     SLFEENGVLDPRTGERFWSEILGVGGSRPALESFVAFRGREPRMDALLRHNGMVERA"
     gene            5363..6181
                     /locus_tag="AC158Q_07935"
     CDS             5363..6181
                     /locus_tag="AC158Q_07935"
                     /inference="COORDINATES: protein motif:HMM:NF012675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HIR3339746.1"
                     /translation="MKTAAFGICVLSAVSGAVLAAQLYRWVDERGNVEWRDTPPPASA
                     KKVETRKVGGGTVPAAELPYSVQLAVKNFPVTLWTTDCGDACAKARAHLARRGVPHTE
                     KDPRSDFENFKKLTGGAEVPVLFVGGNRLKGYLEGDWDSALDTAGYPRTALVKPQPKV
                     QPKPAPDDKGAPAEKGAEAPAAPAPSSGVKLYTTADCGPNCTNAKQLLGDRGVSFQEI
                     AVENPPQIEELKNMTGDTIVPVLLVGQFWLQGYNAEDYESALDKAGYKRQAAPQ"
     gene            6197..>6518
                     /locus_tag="AC158Q_07940"
     CDS             6197..>6518
                     /locus_tag="AC158Q_07940"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006712787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HIR3339747.1"
                     /translation="MKLATWNVNSLKVRLPQVLEWTARHSPDALCLQETKLDSPKFPE
                     AEIEAAGYRALHNGQKTYNGVAIVSRAPPGETILEIPGFEDPQRRVLAATIGGVRIIN
                     VYVPN"
BASE COUNT         1197 a         2172 c         2130 g         1019 t
ORIGIN      
        1 atcgtccctg cgcgccaccg gcgcgcgcgg cacgcggctc aaggccggcc tcgacgcggt
       61 ggccgcccag cgcatactgg aagcctattt cgcgggcgcg aaataggtca tggtgattgg
      121 gtgattaagt gaccaagtga ttcggttaga ttcagacagc ccattgtgcc cgagcgactc
      181 aatcactcaa tcacctaatc actcaattac tcattaccgc tcacacgtta gcggtgattt
      241 atggtagtct tgcaccccgc gaaaacattt aaaacatgcc cggcaatccg ttacctgacg
      301 ccgagcagct gctgaaagcg ctgatcgagc agatgaggcc cgcaatcggg ccggaaacgg
      361 gtttgatcgg catccatacc ggcggcgtct gggtcgccga acgcctgcac caggcgttga
      421 agctgcaaac gccgctcgcc accctcgacg tctccttcta ccgcgacgat taccaggccc
      481 gcgggctgca ccgcgacgtc aagcgctccg aaatcccgtt cgaggtcgag ggccgcgatc
      541 tcatcctggt cgacgacgtg ctctacaccg ggcgcaccat ccgcgcggcg ctcaacgaaa
      601 ttttcgatta cggccggccg accgccgtgc ggctcgccac gctggtggac cggggcgggc
      661 ggcaactgcc gattgccgct caattcgtgg gcgccacgct cgctctcacg cccggccgga
      721 gcgtcggctt gaagcgcgac gccaggggac gcctcgagct cgaaatgcgc gcggaaaaat
      781 cctgatgtgg ctccaccctt ccaaccccca gctcgacaag aaagggcagc tgcatcacct
      841 gctcacgctg gacgggctgc cggccgagat cctgtggcag atcctcgaca cggccaagtc
      901 cttcgtcggc accagggtca agaaagtccc gctgctgcgc ggcaaatccg tgttcaacgt
      961 gttcttcgag ccgtccacgc gcacgcgcac gaccttcgag atcgccgcga agcgcctctc
     1021 ggccgacgtc atcaacctca acgtcaccgt gtcctcccaa tccaagggcg agagcctgct
     1081 cgacacggtg gacaacctgt cggcgatgca cgccgacatg ttcgtggtgc ggcacggcga
     1141 gagcggcgcg ccgcatttca ttgcgcgcca cgtcgcgccc gacatccacg tgatcaacgc
     1201 cggcgacggg cgccattcgc acccgacgca gggcctgctc gacatgtaca ccatccgcca
     1261 ctacaagaag gatttctcga acctgcgggt ggcgatcgtg ggcgatgtcc tgcactcgcg
     1321 ggtggcgcgc tcgcagatcc acgcgctcac cacgctcggc tgcccggaga tccgcgtgat
     1381 cggaccgaag acgctgctgc ccgcgcacgt cgagcagctc ggcgtgcacg tctaccacga
     1441 catgcgccag gggctgaagg acgtggacgt ggtgacgatg ctgcggctgc agaacgagcg
     1501 catgcagggg gcgctgctgc cgtcggccca ggagttctac aagttctacg gcctgaccga
     1561 cgacaagctc gcggtcgccc ggccggacgc catcgtgctc caccccggcc cgatgaaccg
     1621 cggcgtggaa atcgattcca gcgtcgcgga cggaaagcaa tcggtgatcc tgccgcaagt
     1681 caccttcggc atcgccatcc gcatggcggt gatgagcatc atggcaggca acaagtgagt
     1741 gatgagtttt tagtgttgcg tgttgagttg aaggcaaaac cgctcaatgc atcgcactta
     1801 actcaaaact caacattcaa aactcaacac tgatatgaag attcatatca aaggcggccg
     1861 gctgatcgac ccgaagagcg gcgtggacgc gaagaaggac gtctatatca ccgcgggcag
     1921 gatcgtctcg atcggcgacg cgccgaacgg ctataccgcc aaccgcgtca tcgacgcggc
     1981 gggcctggtc gtctgccccg gcctggtgga cctcgcggcg cggctgcgcg agccgggctt
     2041 cgaatacatg gcgacgctgg actccgaaat ggaggcagcc atcgccgggg gcgtgaccag
     2101 cctcgcctgc ccgcccgaca ccgacccccc gctggatgag cccgggctgg tcgagatgct
     2161 caagttccgc gcgaagaacc tgaaccaggc gcgcgtctat cccgtcggcg cgctcaccca
     2221 gggcctcaag ggcgcgcgcc tgaccgagat ggtggagctg gtggacgcgg gctgcgtcgc
     2281 tgtctcgcag gcggacgcgc cgttcgccga cacccaggtg ttctaccacg cgctccagta
     2341 cgccgcgacc ttcggcttcc cggtatggct gcgcccgcag gatcccgcgc tggcgcagca
     2401 cggcgtcgcc cacgacgggc cggtcgccac gcgcctgggg ctgccggcca tacccgtgtg
     2461 cgccgagacc gtggcgctct ccacgatact gatcctcatg cgcgcgaccg gcgcgcgcgt
     2521 gcatctcgcg cggctgtcca gcgccgaggg cgtggagatg gtgcgccagg cgaaacacga
     2581 gaaattgccc atcagctgcg acgtcgccat ccatcatgcc cacctgaccg agatggacat
     2641 cggctacttc gactccaaca gccggctcga cccgccgctg cgcagccagc gcgaccgcga
     2701 cgcgctgcgc gcggcgcttg ccgacggcac ggtggacgcg ctgtgctcgg atcacacgcc
     2761 ggtggacgcg gacgtgaagc agctgccgtt cgccgaggcc gaggccggcg cgaccggact
     2821 cgaactgctg ctgccgctca cgctcaagtg ggcggaggaa tcgaaggtgc cgctggccac
     2881 cgcgctcgcg cggatcacgt ctgaggcggc gcgcgtcttg aacgtggacg ccggccacct
     2941 caccccgggg cacgccgccg acatctgcat cttcgacccc ggccgccact ggaaggtcga
     3001 gcccgccgcg ctgaaaagcc agggcaagaa caccccgttc ctcggcctgg agctcaaggg
     3061 ccgggcacgc tacaccctgg tcgacgggca agtggtctac gaaaccaaat aaccggttcc
     3121 aagtttcaag ttacaagttg caagatgcaa agctggatgg ttgccgagcc ttgcttgaaa
     3181 cctggaactt ggaacttgta actcaatctt atagatcgtc atgaatcccc ttctcgactt
     3241 ctccggcctg ccccggttcg ccgatttcaa tcccgggctg atcacgccgg cggtggagac
     3301 gctgctcgcg gagaaccggg cgctgatcga gcgcgtcgtg gacccgggcg tgcccgccag
     3361 ctggcgcgat ttcgtgcagc cgctggagga cgccaacgag cggctgcgcc gggcatgggg
     3421 cgtggcgggg cacctcaacg cggtgatgaa cagccccgag ttgcgcgagg cctacaacgc
     3481 caacctgccg aaggtcacgc agtactacac cgagctcggc cagcacgaag ggctctacgc
     3541 caagttcaag gcgctcggcc gcgggccggg attcgagggg ttgacgcgcg cgcagaagaa
     3601 gatcgtcgag aacgagctgc gcgatttccg gctgggcggc gcggagctgc cgcccgggaa
     3661 aaaggcgcgc ttcaaggagg tgagcgagaa gctgtcgcag ctctcgtcgc gtttttccga
     3721 caacctgctc gatgccacca acgcgttcgc gcacgacatc accgatccgg cggaagtttc
     3781 cggcatcccc gaagacgtgc tggcggccgc gcgcgcggcg gcgcaagccg acggcaagac
     3841 cggctggaaa ttcaccctgc atgccccctc ctacctgccg gtcatgcagt acgcggaccg
     3901 gcggagcttg cgcgagctga tgtaccgggc ctatgtcacg cgcgcctccg agttcggaaa
     3961 accggagtgg gacaacacgt ccctcatcgc cgggatactc cagctgcggc aggaactggc
     4021 gcggctcatc gggtttgcca gttacgcgga atactcgctc gagcccaaga tggcggagtc
     4081 gccgcagcag gtgctcgatt tcctcggcga actcgcggcc cgggcgaaac cctacgccga
     4141 acgcgacctg aaggaggtga cggagttcgc ccgtgccgag ctcgggctgc cgaagctgga
     4201 gtcctgggac ttcgcctacg cctcggaaaa gctgcgggcg aagcgctatg ccttttccga
     4261 ccaggaagtg aagcagtact tcccggagac gcgggtgctg cccggcatgt tcaaggtggt
     4321 ggaaacgctc tacggcctca cgatcgcgcc ggccgacgcg ccggtgtggc accccgacgt
     4381 gcgcttctac gcggtacgcg accgcgccgg cgggctggtc gggcaatttt acgtggacct
     4441 ttacgcgcgc ccgtccaagc gtggcggcgc gtggatggac gatgcggtcg cgcggcggct
     4501 caaggacggg cgcgtgcagg cgccggtcgc ttacctgaac tgcaacttct cggcgccggt
     4561 cggcggcaag ccggcgctgt tcacgcacga cgaggtcaac acgctgtttc acgagttcgg
     4621 ccacggcctg caccacctgc tgacgcgggt ggattacctg ggcgtctccg gcatcaacgg
     4681 cgtggaatgg gacgcggtcg agctgccctc ccagttcatg gaaaatttct gctgggaatg
     4741 gggcgtgctc gcgccgatga ccgcccacgt ggacaccggc gcgccgctgc cgcgcgcgct
     4801 gttcgacaag atgctcgccg ccaggaattt ccagagcggg atgcagatgg tgcgccagct
     4861 cgagtttgcg ctgttcgacc tgcgcctgca ccacgattac gatcccgccg atacgaaaac
     4921 gggcgcgaag accgtgctcg aactgctcga cgaggtgcgc cggcgcgtcg cggtcgtgtt
     4981 cccgccggac tacaaccgct tcccgcacaa cttctcgcac atcttcgcgg gcggttacgg
     5041 cgcgggatac tacagctaca aatgggccga ggtgctgtcg gcggatgcct acagcctgtt
     5101 cgaggaaaac ggcgtgctcg acccgcgcac cggggagcgc ttctggagcg aaatactcgg
     5161 cgtgggcggc agccgccccg cgctggaatc cttcgtcgcg tttcgcgggc gggaacccag
     5221 gatggatgcc ttgctccggc acaacgggat ggtggagcgc gcctagccga ggttgaaatg
     5281 tgaaagggtt cacgccgcgc ctttgcaccg ggtaggccga agtgctagag tcaattcagg
     5341 caataactca taaggttgcg tcatgaaaac agccgcgttc ggaatttgcg ttctgtccgc
     5401 cgtctccggc gccgttctcg ccgcgcagct ctaccgctgg gtggacgaga ggggcaacgt
     5461 cgagtggcgc gatacgccgc cgcccgcatc ggccaagaag gtcgagaccc gcaaggtcgg
     5521 cggcggcacc gtgccggcgg ccgagctgcc gtacagcgtc cagttggcgg tgaagaactt
     5581 cccggtcacg ctgtggacga ccgattgcgg cgatgcctgc gccaaggcgc gcgcccacct
     5641 ggcgcgccgc ggggtgccgc acacggagaa ggacccgcgc agcgatttcg agaacttcaa
     5701 gaaactgacc ggcggcgccg aagtcccggt gctgttcgtg ggcggcaacc ggctgaaggg
     5761 gtatctcgag ggcgactggg attcggcgct ggatacggcg ggctacccca gaacggccct
     5821 ggtcaagccg cagcccaagg tgcaacccaa accggccccc gatgacaagg gcgcgccggc
     5881 cgagaaagga gcggaggcgc ccgccgcgcc cgcgccctcg tcgggagtga agctgtacac
     5941 gaccgccgat tgcggcccga actgcaccaa tgccaagcag ctgctgggcg accgcggcgt
     6001 gagcttccaa gagatcgcgg tcgagaatcc gccgcagatc gaggagttga agaacatgac
     6061 cggcgacacc atcgtgccag tgctgctcgt cgggcagttc tggctgcagg gctacaacgc
     6121 cgaggactac gaaagcgcgc tcgacaaggc aggctacaag cgccaggccg cgccgcagta
     6181 gaagcgcggc cggccggtga agctcgcgac ctggaacgtc aactcgctca aggtcaggct
     6241 gccgcaggtt ctcgagtgga cggccaggca tagccccgac gcgctgtgcc tgcaggagac
     6301 caagctcgat agcccgaagt ttcccgaggc cgagatcgag gccgccggct atcgcgcgct
     6361 ccacaacggc cagaagacct acaacggcgt cgccatcgtt tcgcgcgcgc cgccgggcga
     6421 aacgatattg gaaattcccg gattcgaaga ccctcagagg cgggtgctgg cggcgaccat
     6481 cggcggggtc aggatcatca acgtctacgt ccccaacg
//
LOCUS       DBNGSG010000305         2016 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1796335, whole genome shotgun sequence.
ACCESSION   DBNGSG010000305 DBNGSG010000000
VERSION     DBNGSG010000305.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1796335"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..>2016)
                     /locus_tag="AC158Q_07945"
     CDS             complement(9..>2016)
                     /locus_tag="AC158Q_07945"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HIR3339748.1"
                     /translation="PRDRLALLVDKLFDKYEIAVLGFDVVFAEREESSGIRVLERLGT
                     RELRDVPQYQDVLRRVKPQLEYDDIFARRMKGRPVVMGYIFSGEEQGLAPKKGVLPPP
                     IFTAESFAARPIRTTSWNGYTSNLEVLQKNAANAGHFNSHTDDDGIVRRVPMLAEYNG
                     AYYEPLSLAMVRVLLGSPPVKAISPDGALAPEGYPDLEFLEVGSLRIPVDDTASALVP
                     YRGRKFSYKYHSVVDVINDRIDPSELKGKIVIVGTTAPGLLDLRATPVDAVYPGVEVH
                     ANMIAGMLDGNIKQRPPYVVGAEFVLLLLSGLVLALLLPLLNPLRLTLVTILVLTAVL
                     VGNVMVFEHGHLVLPIASGAVMILLLYTLNMAYGFFVEARGARLITGLFGQYVPPELV
                     EKMAENPEQFNMAPRAEELSVLFSDVRGFTTISESLTPEDLSHYINEYLTRMSLVIRE
                     RHRGTLDKYIGDAVMAFWGAPVADRQHARNAVLAALDMQKEAVGLNEEFKAKGWPTFA
                     IGIGVNSGVVRVGDMGSKIRKAYTVMGDAVNTASRLEGITKQYGADIIIGEGTHKLIS
                     GFVCREVDRVRVKGKEEPVAIYEPFGLEGQVDKAKLDEIAVWNQALEYYRTQQWDLAE
                     MQLINLQKMDPEVELYDIYIERVAIFRKEPPGDGWDGVWKFETK"
BASE COUNT          404 a          670 c          557 g          385 t
ORIGIN      
        1 aagctccatc acttggtctc gaatttccag acaccatccc acccgtctcc gggaggctcc
       61 ttgcgaaaga tggcgacccg ctcgatatag atgtcataca actcgacctc gggatccatc
      121 ttttgcaagt tgatcagctg catttcggcc agatcccact gttgcgtgcg gtagtattca
      181 agcgcttgat tccaaacagc aatttcgtca agcttggcct tgtcaacctg gccctccaat
      241 ccgaacggct cgtagatcgc aaccggttcc tccttgccct tcacccgcac ccggtcaacc
      301 tcgcggcata cgaagcccga aatcagcttg tgggtgccct ctccgataat aatatcagcc
      361 ccatactgtt ttgtgatgcc ttcgagccgc gaggcggtgt ttaccgcgtc gcccatcaca
      421 gtgtaagcct tgcgaatctt cgagcccatg tcgccgacgc gcaccacgcc cgaattcact
      481 ccgatgccga tcgcgaatgt cggccagccc ttcgccttga actcctcgtt gagaccgacg
      541 gcttccttct gcatatccag cgccgcgagc acggcattcc tcgcgtgttg acggtcggca
      601 acgggagcgc cccagaacgc catcaccgca tcaccgatgt acttgtcgag cgtgccgcga
      661 tgccgctccc ggatcaccag gctcatgcgg gtaagatact cattgatgta gtgggacagg
      721 tcctcgggag tcaggctttc cgaaatggtc gtgaatccgc gcacatccga aaacagcacc
      781 gaaagttcct ccgcgcgcgg cgccatgttg aactgttccg ggttttccgc catcttctcc
      841 accagttccg gcggcacata ctggccgaac aggccggtta tgaggcgcgc gccacgcgcc
      901 tcgacgaaga aaccgtaagc catgttgagc gtatacagca gcagtatcat caccgcgccc
      961 gaggcgatcg gcagcaccag gtggccgtgc tcgaacacca tcacgttacc gaccagcact
     1021 gccgtcagca ccagtatggt caccagcgtc agcctgagcg ggttgagaag cgggagcagc
     1081 agcgccagga ccaggcctga cagcagcagc agaacgaact cggcgcccac cacgtacggc
     1141 ggacgctgct tgatgttgcc gtcgagcatt cccgcaatca tgttggcgtg cacctcgacc
     1201 ccggggtaaa ccgcatccac gggggttgcc ctcaggtcga gcaggccggg ggccgtcgtg
     1261 ccaacgatca cgattttgcc cttcagctcg gacggatcga tacggtcgtt gatcacgtcg
     1321 acgaccgagt gatacttgta gctgaacttg cgcccgcgat agggaaccag agcgctcgcg
     1381 gtatcatcca ccggtatcct gagtgagccc acctccagaa actccaggtc gggatagcct
     1441 tcgggcgcga gcgccccgtc cggggaaatc gccttgaccg ggggcgagcc gagcaggacg
     1501 cgcaccatcg ccagcgaaag aggctcgtaa taagcaccgt tatactcggc cagcattggc
     1561 acgcgccgca cgatcccgtc gtcgtcggta tgcgaattga aatgccccgc attggccgcg
     1621 ttcttctgca gcacttcgag gttcgacgtg tagccgttcc aggaggtggt ccggatcggc
     1681 cgcgcggcaa acgattcggc ggtgaatatc ggcggcggca gcacgccctt cttcggcgcc
     1741 agcccctgct cttcgccgga aaaaatatac cccatcacca ccgggcggcc cttcatcctt
     1801 cgcgcgaaga tgtcatcgta ttcgagctga ggtttgaccc gcctcaacac atcctgatac
     1861 tgcgggacat cgcgcaattc ccgcgtgccc aggcgttcga ggacgcggat gccggaagac
     1921 tcttcgcgct ccgcaaacac cacatcgaac ccgagcaccg cgatctcgta cttgtcgaac
     1981 agcttgtcga ccagcagcgc caggcggtca cgcggc
//
LOCUS       DBNGSG010000306         8198 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2137920, whole genome shotgun sequence.
ACCESSION   DBNGSG010000306 DBNGSG010000000
VERSION     DBNGSG010000306.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 8198)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8198)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8198
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2137920"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="AC158Q_07950"
     CDS             complement(<1..292)
                     /locus_tag="AC158Q_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339749.1"
                     /translation="MKPVNPAHFLLIVSALASVAAVAQQFPPVPAPAEGRSSQPAKPL
                     SGTTLLSELRKGGYVIYFRHTSTDMSRDDSRSRSEDDCGNQRPLTDRGREEAR"
     gene            412..1824
                     /locus_tag="AC158Q_07955"
     CDS             412..1824
                     /locus_tag="AC158Q_07955"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012612.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HIR3339750.1"
                     /translation="MKVTRRAALKSLAALAAASWYAPARAAEARLTAGVARQQVVPAE
                     YPQTEVWSYNGAVPAPELRFRQGETARIVVANRLPQPTTVHWHGLRVPNRMDGVPHLT
                     QPPIAPGSEFVYEFPLPDAGTFWYHPHLASYEQVARGLYGVLVVEERKPIEADRDVTW
                     VLSDWRFNADASQREDFGSVFDLTHGGRIGNTVTINGRFTLKDGVFEVRSGERIRLRL
                     VNTAVARIFLLRFSGHEPRVIALDGQPVEPHAPERGLIALAPGMRADIVLDCMERPGS
                     SVAVTDEYNPRAESSLITLAYRDEPPLRDRPSAAPVSLAPNPLPEPDPARAQRHEIVF
                     QGGAMGTLRDAQYAGSRVPLAVLVKQHHLAWTVNGVAVKEHVHEPMLVLKRNGHFVLA
                     MKNDTAWPHPIHLHGHSFRVIGRDGKPTRHREWRDTVLMGPREAVDIAFVADNPGDWM
                     FHCHILAHQAGGMMAMFRVV"
     gene            2033..2932
                     /locus_tag="AC158Q_07960"
     CDS             2033..2932
                     /locus_tag="AC158Q_07960"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3339751.1"
                     /translation="MPVRTLKDWGLLAALVALWGSNFMFVKIGVATVPPATLVASRLV
                     IGAVILVAVVRAMGHTFPPLGRIWIPYVVLAVVGNSIPFWLITTGQQSLDSALAGILM
                     AIMPLTTLVLAHFFVAGERMTRNRLAGFLLGFAGIIVLMGPAALSGLGGSPVAILAQL
                     CVLAGALCYATQSVLVRMMLKGNVMVASAAIIAIAALISLPIALVVDQPWELAPSRAS
                     VYTVIWIGIGPTALATIVYLKLIGSAGPTFMSLTNYCIPVVALLLGMALLGEEPGANA
                     YTGLLMILSGIAISQLRRSASRR"
     gene            3148..3387
                     /locus_tag="AC158Q_07965"
     CDS             3148..3387
                     /locus_tag="AC158Q_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008351849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTH_I0359 family protein"
                     /protein_id="HIR3339752.1"
                     /translation="MTMNIIYASDNYYVVEYPGQHGYELVDKRSQRGTFFQGDVADRF
                     KQSLQAAVAEEASVERVDEFLGGFDILLNQPLVVH"
     gene            complement(3478..4215)
                     /locus_tag="AC158Q_07970"
     CDS             complement(3478..4215)
                     /locus_tag="AC158Q_07970"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF005451.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HIR3339753.1"
                     /translation="MTSKNELEARIDLAAALRWADRLGLGEGICNHFSVEIPGRPGHF
                     LINPQGLHWSEVTPGDLVVVDAAGRKVEGRYGVEPTAFFIHGAMHRSKPSAKCVMHTH
                     MPYATTLTVVHGGRLEWASQNSLKFHGRVAYDDNYNGLALDEAEGSRMCAQAREADVL
                     FLANHGVIVCGPTVAAAFDDLYYLERACMLQVLAMGTGKPLRMVPERIAGLTAEQMAQ
                     DSEQTGLHFAAIKRMLDRDAPGWSRLD"
     gene            complement(4291..5679)
                     /locus_tag="AC158Q_07975"
     CDS             complement(4291..5679)
                     /locus_tag="AC158Q_07975"
                     /inference="COORDINATES: protein motif:HMM:NF013122.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HIR3339754.1"
                     /translation="MLDQPLAFLDLETTGINAVHDRITEVGLIEIGPGDRITEWSTLV
                     NPGVRIPPTIQNITGITDDMVALAPAFSEIARPLLARLDGKLLVAHNARFDYGFLRNE
                     FRRAGHRYASRVLCTVKLSRKLYPHEARHNLDALMARHDVACEARHRALGDARAIWLL
                     LQRWRRELGPERLAAVVADLVKTPAVPAGLPEGALDDIPEAPGVYLFHGENGVVLYVG
                     KSINLRSRVMAHFSGDHRDYRDMKISQQVRRVNWIETPGELGALIEEARLIKQLAPVY
                     NRRLRRIAELCAWHWQAEVPASPPRLVTARELDETGLGDLYGLFRSRAAALETLREVA
                     AAHHLCPIVLGLEKGAGPCFAHQLKRCRGACVGKETRLAHGLRLGAALAQLRMRPWPF
                     KGRIGVRERRAGALIVLDRWCYLGTVSSEGELAELAQQRPRPGFDLDTYKILTRFFAS
                     SRRDYQVVELAA"
     gene            complement(5807..6529)
                     /locus_tag="AC158Q_07980"
     CDS             complement(5807..6529)
                     /locus_tag="AC158Q_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIR3339755.1"
                     /translation="MMSLLDALARHATPVNLKQLATETRLHPSTAHRILSVMVDNRLV
                     DRIEPGTYRLGIRLLELGSLVKSRINVRQEALPHMQRLHQELGETVNLSVRHDDEVVY
                     IERTSGNSSMMRVVQIIGARAPLHITAVGKVFLAEDSADQSADYARRTGLPRYTDNTL
                     TDADSLAKELEKIRRQGYALDEEEAEKGVSCIGAGIFNDEGRLVAGLSVSAPSDRLNK
                     GWAAQVRQTAEKISRAIGHQRQ"
     gene            6932..7405
                     /gene="greB"
                     /locus_tag="AC158Q_07985"
     CDS             6932..7405
                     /gene="greB"
                     /locus_tag="AC158Q_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HIR3339756.1"
                     /translation="MTPLGYARLKAELTKLLDSERPDLVKTISWAASNGDRSENGDYI
                     YGKKRLREIDRRIHYLNKRLDSAEVVDPAKRGESDQIYFGATVTYAYVRGGKDERTVT
                     IVGVDEVDTKRGQVSWLSPIARVLLKRRSGDIVTLATPSGEAEIEIIGVRYRAPA"
     gene            7666..>8198
                     /locus_tag="AC158Q_07990"
     CDS             7666..>8198
                     /locus_tag="AC158Q_07990"
                     /inference="COORDINATES: protein motif:HMM:NF024758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HIR3339757.1"
                     /translation="MTVSFFAAALSSWRAAHLSVPELRGDDISQAAVVKLSIKAVPNA
                     RTSENLGAEREGTGVVFGEKGLILTIGYLILEADSILIVAGDGRVYPASVIGYDHATG
                     FGVLRASVDCRPLELGSSSGVRELSTVLVAAHGAAGGTSRACVVSRRRFTGWWEYMIE
                     GGIFTAPPRFEHSCAALL"
BASE COUNT         1408 a         2598 c         2689 g         1503 t
ORIGIN      
        1 cccgcgcttc ctcgcggccc cggtcagtca aggggcgctg gttgccgcaa tcatcctcgc
       61 tcctcgagcg cgagtcgtcg cgcgacatgt cggtcgaggt gtggcggaag tagatgacgt
      121 agccaccctt gcgcaactcg gaaagcagcg tggtcccgga caacggtttc gcgggctggg
      181 aactgcgccc ctcggcgggc gcggggaccg gcgggaactg ctgcgccacc gccgcaacgg
      241 aggccagcgc agaaacaatc aacaggaagt gggcaggatt cacaggtttc ataagattag
      301 gctggaagag aaactctgta aatctccgct gtctgaccgg aacattatcg gcgttccctt
      361 cggcaggctc aggacaggca tcggcggctc attgtgttat atccttcgcg catgaaagtc
      421 acccgccgcg ccgcattgaa aagcctcgcc gccctggccg ccgcgtcctg gtacgcgccg
      481 gctcgcgccg ccgaggcaag gctgacggcc ggcgtcgcac ggcagcaggt cgttcccgcc
      541 gagtacccgc agaccgaggt atggagctac aacggcgcgg tgccggcgcc cgagctgcgc
      601 ttccggcagg gcgagaccgc gcgcatcgtg gtcgccaacc gcctgccgca gccgaccacc
      661 gtgcactggc acgggctgcg cgtgcccaac cgcatggatg gcgtgccgca cttgacgcaa
      721 ccgccgatcg cgcccggcag cgaattcgtt tacgaattcc cgctgcccga tgccggcaca
      781 ttctggtacc acccgcatct cgcgagctac gagcaggtcg cgcgcggcct ctacggcgtc
      841 ctggtcgtcg aggagcgcaa gccgatcgag gccgaccgcg atgtcacctg ggtgctgtcg
      901 gactggcggt tcaatgccga tgcctcgcag cgcgaggatt tcggcagcgt cttcgatttg
      961 acgcacggcg gccgcatcgg caatacggtg acgatcaacg gccggttcac gctgaaggac
     1021 ggcgtgttcg aggttcgcag cggcgagcgt atccgcctgc ggctcgtcaa caccgccgtc
     1081 gctcgcattt tcctgctgcg gttttccggg cacgagccac gggtaatcgc gctcgacggc
     1141 cagccggtcg aaccccatgc gccggaacgg ggattgatcg cgctggcgcc gggcatgcgc
     1201 gcggacatcg tcctcgattg catggagcgg ccgggatcga gcgtcgcggt gaccgacgag
     1261 tacaatccgc gcgccgaatc gagcctgatc acgctggctt accgcgacga gccgccgctg
     1321 cgagaccggc cgtccgccgc cccggtctct cttgcgccca atccgctgcc ggaacccgac
     1381 ccggcgcggg cgcaaaggca cgaaatcgtg ttccagggcg gtgccatggg aaccctccgg
     1441 gatgcgcagt atgcgggcag ccgcgtgccg ctggcagtgc tggtgaagca gcaccacctt
     1501 gcctggaccg tcaatggcgt cgcggtgaag gagcacgtgc acgaaccgat gctcgtcctg
     1561 aagcgcaacg gccatttcgt gctcgcgatg aaaaacgaca ccgcatggcc gcacccgatc
     1621 cacctgcacg gacactcttt tcgcgtcatc gggcgggacg gaaaaccgac gcggcaccgg
     1681 gaatggcgcg acacggtgct gatggggccg cgtgaagcgg tggacatcgc cttcgttgcc
     1741 gacaaccccg gtgactggat gttccactgc cacatccttg cccaccaggc gggcgggatg
     1801 atggccatgt tccgcgtcgt ttaacaatag ccgccaagaa gcacggaata aatccgaatg
     1861 gggaggacgc ggcttcgtgt attcttgcgc gtttccggtc acgaagaagc gctggagggg
     1921 atctgaaaca tcgtgctttc ttgcgttcgt tcgctttgac tgaaattgtg ttcgattatt
     1981 ttgctttcct tggcgttctt ggcgccttgg cggctaattt aatgcgttgt gcatgccggt
     2041 ccgcacgctg aaagactggg gcctgctcgc cgcgctagtc gcgctgtggg gctcgaactt
     2101 catgttcgtc aagatcggcg tcgcgaccgt gccgcccgcg acgctcgtcg catcgcgcct
     2161 ggtgatcggc gccgtcatcc tggtcgcggt ggtgcgggcg atgggccaca cctttccgcc
     2221 gctggggcgc atctggattc cttatgtggt gctcgccgtc gtcggcaaca gcattccgtt
     2281 ctggctcatc accaccggcc agcagtccct cgacagcgcg ctggcgggca tcctgatggc
     2341 gatcatgccg ctcaccacgc tcgtgctcgc ccattttttc gtcgccggcg agcgcatgac
     2401 gcgcaaccgc ctcgcgggct ttctgctggg tttcgcgggg atcatcgtgc tgatggggcc
     2461 ggcggccctg agcgggctgg gcggatcacc ggtcgcgatc ctggcccagt tgtgcgtgct
     2521 ggcgggcgca ctttgctacg ccacgcaaag cgtactggtt cgcatgatgc tgaagggcaa
     2581 cgtgatggtg gcgtccgcgg cgatcatcgc cattgccgcg ctgatcagcc tcccgattgc
     2641 gctcgtggtg gaccagccgt gggagctcgc gcccagccgg gcgtcggtct ataccgtgat
     2701 ctggatcggc atcggaccga ccgcacttgc gaccattgtc tatctcaagc tcatcggctc
     2761 ggccggcccc accttcatgt ccctgaccaa ctactgcatc ccggtcgtcg ccctgctgct
     2821 cggtatggcg ctcctcggcg aagagcccgg cgccaacgcc tacaccgggt tgctgatgat
     2881 tctttcgggt attgccatca gccaactccg ccgctccgcc agcaggagat aagtgacaag
     2941 cgacacgaat tcccacgata caattgttga cacttgtaac catcacctgt cacgcatttc
     3001 cctcaagccg tttgttgctt gaaaacaacc gttcgcgccc ccagataccg ctagtgtgag
     3061 ttaattcacg gccctgtttg aacatggcgg ttccggccgt gtcatcctgt gagggtggtg
     3121 agcgttgtat ctggctatga ggcggacatg acgatgaaca tcatttacgc gagcgacaac
     3181 tattacgtgg tggaatatcc cggccagcac ggctacgagc tggtggacaa gcgcagccag
     3241 cgcgggacgt tcttccaggg cgacgtcgcg gaccggttca agcagtcgct tcaggccgcg
     3301 gtggcggagg aagcttccgt cgagcgcgtg gacgaattcc tcggcggctt cgacatcctg
     3361 ctcaaccagc cgctggtggt gcactgacgg caggtacaag atacaagggg caaggtacaa
     3421 gaagaagcat ggacacgccg ttccttgaac cttgtaactt gcaacttgta gctgaggtca
     3481 gtcgagacgt gaccacccgg gagcgtctcg atcgagcatc cgcttgatcg ccgcaaaatg
     3541 cagcccggtc tgctcgctgt cctgggccat ctgctccgcg gtcaagccgg cgatgcgctc
     3601 cggcaccatg cgcagcggct tcccggtgcc catcgccaat acctgcagca tgcacgcgcg
     3661 ctccaggtag tagaggtcgt cgaaagcggc cgcgacggtc ggcccgcaca cgatgacgcc
     3721 gtggttggcg aggaacagca cgtcggcctc ccgcgcctgc gcgcacatgc ggctgccttc
     3781 cgcttcatcc agcgcgagcc cgttgtagtt gtcgtcatag gcaacgcgcc cgtggaattt
     3841 aagcgaattc tggctcgccc attccaggcg gccgccgtgc accacggtga gcgtggtggc
     3901 atacggcatg tgggtgtgca tcacgcattt cgctgacggc tttgaccggt gcatggcgcc
     3961 gtggatgaaa aaagcagtgg gctcgacgcc gtatcggccc tccaccttgc gcccggcggc
     4021 atccactacc acgagatcgc ccggcgtcac ttccgaccaa tgcagcccct gggggttgat
     4081 caggaaatgt ccggggcgtc ccgggatttc cacgctgaag tgattgcaga tgccctcgcc
     4141 caggccgagc cggtcggccc agcgcagagc cgccgcgaga tcgatccgcg cttcaagttc
     4201 gttcttgctt gtcatgccgg cctggctcga aatcgtggtt tccaggcaat tctaacccgc
     4261 tcgagatgac ggggcccggc acccggcgtt tcaggcggca agctcgacga cctggtagtc
     4321 gcggcgtgaa ctcgcgaaga aacgcgtgag tatcttgtaa gtatcgaggt cgaaaccggg
     4381 tcgcgggcgc tgttgcgcga gttcggcgag ctccccctcg gaactgacgg tgccgagata
     4441 gcaccagcga tcgaggacga tcagcgcccc ggcgcggcgt tcccgcactc caatgcgccc
     4501 cttgaacggc cacggccgca ttctgagttg cgcgagcgcg gcgccgagac gcaggccgtg
     4561 ggcgagacgg gtctccttgc ccacgcaggc gccgcggcag cgcttcagct ggtgggcgaa
     4621 gcacggtccc gcgcctttct ccagcccaag cacgatgggg cacagatgat gagctgcagc
     4681 gacttcgcgc agcgtctcca gcgcggccgc gcgcgagcgg aacaggccgt acagatcgcc
     4741 gagcccggtc tcatcgagct cgcgcgcggt gaccaggcgg ggaggagacg ccgggacctc
     4801 ggcctgccag tgccacgcgc aaagctcggc gatgcggcgc aggcggcggt tgtaaaccgg
     4861 cgcaagttgc ttgatgagcc gcgcctcctc gatcagggcg cccagctcgc caggggtttc
     4921 gatccagttc acgcgccgca cctgctgcga gattttcatg tcccggtagt cgcgatggtc
     4981 cccggagaaa tgcgccatca cgcgggagcg caggttgatg ctcttgccca cgtacaggac
     5041 gacgccgttc tcgccgtgaa aaaggtagac gcccggcgcc tcgggaatgt catccagcgc
     5101 gccttcgggc agcccggcgg gcacggcggg tgttttcacc aggtcggcca ccacggcggc
     5161 cagccgttcc gggccgagtt cgcgccgcca cctctgcaga agcagccaga tcgcgcgggc
     5221 gtcgcccagg gcgcggtggc gcgcttcaca cgcgacgtcg tggcgcgcca tcagtgcatc
     5281 caggttgtgg cgtgcctcgt gcgggtagag cttgcgcgag agcttgacag tgcaaagaac
     5341 ccggctggca tagcggtggc cggcgcgtcg gaactcgttg cggagaaagc cgtaatcgaa
     5401 gcgggcgttg tgcgcgacga gcagcttgcc gtccagccgg gccagcaggg gccgggcgat
     5461 ctcggagaag gccggggcga gagccaccat gtcgtcggtg atgcccgtga tgttctggat
     5521 cgtcggagga atcctgacgc cggggttcac cagcgtggac cactcggtga tgcggtcgcc
     5581 cggcccgatc tcgatcaggc cgacttcggt gatgcggtca tgaaccgcat tgatgccggt
     5641 ggtctcgagg tccaggaagg caagcggctg atcaagcatt ggttgaaagg cgtgattcgt
     5701 gattggtgat tcgtgattcg agtctattgc ctgggttcac ttccctcgcc tgcctgggca
     5761 ggtcctttac gcgggaacgt cgattcacga ctcacgaatc acgcggttac tggcgttgat
     5821 ggccaatggc ccgcgagatc ttttcggcgg tctgccggac ctgggcggcc cagcccttgt
     5881 tgagccggtc ggagggagcg gaaaccgaga ggccggcgac aaggcggcct tcgtcattga
     5941 agatgccggc gccgatgcag gagacgcctt tctcggcttc ctcctcatcg agagcgtagc
     6001 cctggcggcg gatcttctcg agttcctttg caaggctgtc cgcgtcggtc agggtgttgt
     6061 cggtgtatct cggcaaaccg gtgcggcgcg cgtaatcggc ggattggtcc gcgctgtcct
     6121 cggcgaggaa gaccttaccc accgcggtga tgtgcagcgg cgcgcgcgcg ccgatgatct
     6181 gcaccactcg catcatggaa ctgttgcccg aggtacgttc gatgtaaacg acctcgtcgt
     6241 catgtcgcac agacaggttg acggtctcgc ccagctcctg gtgcagccgc tgcatatggg
     6301 gcagggcctc ctggcgcacg ttgatgcgcg atttgaccag gctgcccagc tccagcaggc
     6361 ggatgccgag gcgataggtg ccgggctcga tgcggtccac caggcggttg tccaccatga
     6421 ccgagaggat acggtgcgcc gtggacgggt gcaggcgggt ttcggtggcg agctgcttca
     6481 gattgaccgg cgtggcgtgg cgggcaaggg cgtccagcag gctcatcatt cgttcgatga
     6541 cctggatcga ggacttggcg gctttttgtg ccacgacggg ggtttgtccg ggagttggca
     6601 ctcgatttta tatactgaaa tccggagcgg caacccaaac cgctgatttc gaggccggat
     6661 tcgcgcccta ccacaggtat cgccagtaat acattgtttt tgctataata tgaggctccc
     6721 ctgcttgttg gacacggtac ccatcatgac aaaggcagtt ctactgtcac gcagtcgcaa
     6781 cggtaatggt cgagttggtc acggccgaaa cggtaacggc agtggccgga acggcaacgg
     6841 aagtggccgg aacggcaacg gaagtggccg gaacggcaat ggccgcagca gtgtcgcaac
     6901 ggaacaggcg gcgcaggaag tcaagaatta catgacgccg ctgggctatg cccggctcaa
     6961 ggccgagctc acgaagctcc tcgacagcga gcgtcccgac ctggtcaaga ccatctcctg
     7021 ggcggcctcc aacggcgatc gctccgagaa cggtgactac atctacggca agaagcggct
     7081 gcgcgagatc gaccgccgca tccactatct caacaagcgc ctcgacagcg ccgaggtcgt
     7141 cgatccggcg aagcgcggtg aatccgacca gatctacttc ggcgcgaccg tgacttatgc
     7201 ctatgtgcgg ggcgggaagg acgagcgcac ggtcaccatc gtcggggtgg atgaagtgga
     7261 caccaagcgt ggccaggtga gctggctctc gcccattgcc cgggtgctgc tgaagcggcg
     7321 ctccggcgac atcgtcaccc tcgctacccc ctccggcgaa gccgaaatcg agattatcgg
     7381 cgtccgctac cgggcacccg cctgagggtt cgaaaacagg cataaaaagg gggcgatgcc
     7441 ccctttttta atgtccggtc cggaccctgc cagcacccgt tgccgggtgg cgggtgcccc
     7501 gataacatgg gttaaatccg gacgcggagc gccggtttaa ctctgcgtgt tggaacggac
     7561 aggaatattg ccgttcaaca cgcggcccaa tccggatgcc gagcgctgct ataacttcgc
     7621 gtgttttaac gggaaaatga tcaccgttca acgcgcacac cgagcgtgac cgtatccttc
     7681 ttcgccgctg cactttcttc ctggcgcgcc gcgcacctat ccgtgcccga gctgcgcggc
     7741 gacgacattt cgcaggccgc ggtggtgaaa ctctccatca aggcggtgcc gaatgcgcgc
     7801 acctccgaga acctgggcgc cgaacgggaa gggaccgggg tggtgttcgg cgagaaaggg
     7861 ctgatcctca ccatcggcta tctcatcctc gaagcggact ccatcctgat cgtggccggt
     7921 gacgggcgcg tttacccggc gagcgtgatc ggctacgacc acgccacggg attcggggtg
     7981 ctgcgtgcca gcgtcgattg ccgcccgctc gaactgggca gttcctccgg cgtgcgtgaa
     8041 ctgtccaccg tgctcgtggc cgctcacggg gccgccggcg gcacctcgcg cgcctgcgtg
     8101 gtgtcgcggc gccgcttcac aggctggtgg gaatacatga tcgagggggg gatcttcacc
     8161 gcgcctccgc gcttcgagca cagttgcgcg gcgctgct
//
LOCUS       DBNGSG010000307         3042 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1798224, whole genome shotgun sequence.
ACCESSION   DBNGSG010000307 DBNGSG010000000
VERSION     DBNGSG010000307.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1798224"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1490)
                     /locus_tag="AC158Q_07995"
     CDS             complement(<1..1490)
                     /locus_tag="AC158Q_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339758.1"
                     /translation="MADKLLIGISAQGAIVADWRGGRIVEYQSFADDEDGQAGYKDYL
                     AQFSGVPVFIMVDAVEEDYRFETLPHAGGSDRADMVGRKLKQHYRNTPYMASWLLGRE
                     PDKRRDDRYLFSALTNPEVIADWLQPVAACDLPLAGIYLLPMVSAAVLEKLSVKATNL
                     LLVAQHPNGLRLTFFRDRQFRLSRLTRGDSGRAENRVRYFAEEISNTRLYLHALRTMT
                     LDEQLTVLMVDRGDELAEVAAGVMRENPSLDCMRLGRQDLAGQLGIAEPLLDASPYLI
                     YLHLLGVKEPPSNLAPASITAGYKRYQARRALFAGCGALAAAAMIWSGANFDQAMALR
                     GETEDAARQTALLSAQYQEVTRQFPQAPTSADNLKKAVDIAQKLREATHNPQRMMTLV
                     SRAVESSPTIVVRGFGWKYGATEIDAEGGSRAAATGGTSAPTVSTSGAPVPLRRESAL
                     IEGEIRPFRGDYRSAIVTINGLAGRLAKEPEVAEVRVVKLPLNVSPT"
     gene            complement(1603..2811)
                     /locus_tag="AC158Q_08000"
     CDS             complement(1603..2811)
                     /locus_tag="AC158Q_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR3339759.1"
                     /translation="MPSYEYKAIDKAGRHARGGVDAVNEVDLELRLRRMGLDLITYKQ
                     VDRRAAGVGSGGRITRQDLINFCFDLEQMIRSGIPIIDGLRDMRDSIDSPRFRDVLTA
                     MTEDMEGGKVLSQCMAAHPEVFDTVFASLVRAGEQTGQLPEVFKNLADNLRWLDELIS
                     QTRRLLILPLLTLAVVLAVLVALLIVLVPQIAQLFKSMGLALPTQTKVLIAVSGFMTQ
                     YWYVVFGLPVVVFVTVVVVVRRSARAAYMWDYAKLRLPLVGSTLQKIILSRFANIFAL
                     MYRSGITVLDAIKTCESVVNNRVISDSLVRAGQQIAAGESLTETFRNLGVFPPLVIRM
                     LRVGENTGALDTALMNITYFYNRDVKESVDKAQKMLGPAMTLVLGGLLAFVVWAVLGP
                     VYDILGKIKF"
BASE COUNT          506 a         1006 c          994 g          536 t
ORIGIN      
        1 gtggggctga cgttgagcgg cagcttgacc acccgcactt cagccacctc cggctctttg
       61 gcgaggcggc cggcaaggcc gttgatggtg acgatcgcgc tgcggtaatc gccgcggaac
      121 ggccggatct cgccttcgat cagcgcgctt tccctgcgca acggcaccgg tgccccggac
      181 gtggataccg tgggcgctga cgttcccccc gtggcggcag cccgggagcc cccttccgca
      241 tcgatttccg tggcgccgta cttccacccg aaccccctga cgacgatcgt ggggctcgat
      301 tccaccgccc ggctgacaag cgtcatcatg cgctgcgggt tgtgagtggc ttcgcgcagc
      361 ttttgcgcga tatcgaccgc ttttttcagg ttgtcggccg aggtcggcgc ctgcggaaac
      421 tggcgcgtga cttcctggta ctgcgcggac agcaacgccg tctggcgcgc ggcgtcctcg
      481 gtttccccgc gcagcgccat cgcctgatcg aagttggcgc cggaccagat catcgcggcc
      541 gcggcgagcg cgccgcatcc ggcaaagagc gcccggcgcg cctggtagcg cttgtagccg
      601 gccgtgatgc tggccggcgc gagattgctt ggcggctcct tcacgccgag caggtgcagg
      661 taaatcaggt acggcgaggc gtcgagcagc ggctcggcga tgccgagctg cccggccagg
      721 tcctggcggc cgaggcgcat gcaatcgagg ctcggattct cccgcatgac gccggcggcc
      781 acctccgcga gctcgtcgcc ccggtcgacc atgagcaccg tgagctgctc gtcgagcgtc
      841 atggtgcgca gcgcgtggag atagagccgg gtattggaga tttcctcggc gaagtagcgc
      901 actcggttct ccgctctgcc gctgtcgcct cgcgtcagcc gggagaggcg gaactggcgg
      961 tcgcggaaaa aggtgagccg caggccgttg ggatgctgcg ccaccagcag caggttggtc
     1021 gccttgacgc tcagcttctc cagcacggcc gcgctgacca tgggcagcag gtagatgccg
     1081 gccagcggga gatcgcacgc ggccacgggc tggagccagt ccgcgatcac ctcgggattg
     1141 gtgagcgcgg agaaaagata gcggtcgtcg cgccgcttgt cgggttcgcg cccgagcagc
     1201 caggaggcca tgtagggcgt gttgcggtag tgctgcttga gcttgcgccc gaccatgtcg
     1261 gcgcggtcgc tgccgcccgc atgcggcagg gtctcgaagc ggtaatcctc ctcgaccgcg
     1321 tccaccatga tgaagaccgg cacgccgctg aattgcgcca gataatcctt gtatccggcc
     1381 tgcccgtcct cgtcatccgc gaacgactgg tactcgacga tcctgccgcc gcgccagtcg
     1441 gcgacgatcg cgccctgcgc cgaaatgccg atgaggagtt tgtcagccat tcttgagtga
     1501 cgagtgacga gtgacgagtg acgggcaacg ggtgccgttg cctgggtaag gcgaaggcgt
     1561 tcgggtcatc gggtcgtcct gactggtcac gatttccgcc ttttaaaact tgatcttacc
     1621 caaaatatca tacaccggcc ccagcacggc ccagacgacg aaagcgagca ggccgcccag
     1681 caccagcgtc atcgccggcc ccagcatttt ctgggccttg tccaccgatt ccttgacgtc
     1741 gcggttgtag aaataggtga tgttcatgag cgcggtgtcc agcgcgcccg tgttctctcc
     1801 cacccgcagc atgcggatga cgagcggcgg gaacacgccc aggttgcgga aggtctcggt
     1861 caggctctcg ccggcggcaa tctgctggcc ggcccgcacc aggctgtccg agatgacccg
     1921 gttgttcacg acggactcgc aggtcttgat cgcatcgagc acggtgatgc cggagcggta
     1981 catgagcgcg aagatgttcg cgaagcgcga caggatgatc ttctgcagcg tggagccgac
     2041 cagcggcagg cgcagcttcg cgtaatccca catgtaagcg gcccgggcac tgcgccgcac
     2101 caccacgacc accgtcacga acaccacgac cggcaggccg aacaccacgt accagtactg
     2161 agtcatgaac ccggacaccg cgatcagcac cttggtctgc gtgggcagcg cgagccccat
     2221 ggacttgaag agctgcgcga tctgcggcac gaggacgatc agcagcgcga cgagcacggc
     2281 gagcacgacc gcgagcgtga gcagcggcag gatcagcagc cggcgggtct gcgagatcag
     2341 ctcgtccagc caccggagat tgtccgccag gttcttgaag acttccggca gttgcccggt
     2401 ctgctccccc gccctcacca ggctcgcgaa aaccgtgtcg aacacctccg ggtgcgccgc
     2461 catgcactgg gagagcacct tgccgccttc catgtcctcg gtcatcgccg tcagcacgtc
     2521 gcgaaagcgc gggctgtcga tcgagtcgcg catgtcgcgc aggccgtcga tgatgggaat
     2581 gcccgaccgt atcatctgct ccaggtcgaa gcagaagttg atgaggtcct ggcgcgtgat
     2641 tctgccgccc gagccgacgc cggcggcgcg gcggtccacc tgcttgtagg tgatgaggtc
     2701 gagccccatg cgccgcagcc gcagctcgag gtccacctcg ttgaccgcgt ccactccgcc
     2761 gcgcgcgtgc cggccggcct tgtctatcgc tttgtattcg taagaaggca ctgaaggtca
     2821 atgatgaatg atgagtgatg agtgatgaac tggaaccgcc cagcttttca ttccgcattc
     2881 atcgttcatc attccgcatt gtcctgtcag ccatggcggc cggtgagatc gatctcgcgc
     2941 gcgacttcga ccaggctggt gctgccttcg accacccgct gcagcccctc ctcggcgagc
     3001 gagcggtagc ccttgccgaa cgccgccaag cgcagttcct tc
//
LOCUS       DBNGSG010000308         3354 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_950645, whole genome shotgun sequence.
ACCESSION   DBNGSG010000308 DBNGSG010000000
VERSION     DBNGSG010000308.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_950645"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..789)
                     /locus_tag="AC158Q_08005"
     CDS             complement(82..789)
                     /locus_tag="AC158Q_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HIR3339760.1"
                     /translation="MVNSELPVVTCLPSSEVVMMVGARLFSIFTGSAVLLVVALALSA
                     ATASAATILAYGDSLSAAYGLPQEQGWVSLLTRRLAAEKFDYKVANASISGETTHGGR
                     NRIEAALKAHRPSIVILELGANDGLRGTTLDATRANLEAIIDACRNAGARVLLVGMRL
                     PPNYGTDYSHKFQQIYVEVARRKKLPLVPFLFEGFADKREYFLEDGFHPAANGQMMML
                     DTVWKELRPLLKKSGKR"
     gene            707..1399
                     /locus_tag="AC158Q_08010"
     CDS             707..1399
                     /locus_tag="AC158Q_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3339761.1"
                     /translation="MLNSLAPTIITTSELGKQVTTGSSELTILSGVSFEVRAGEAVAI
                     VGVSGSGKSTLLGLLAGLDTPSAGAVRIDGHELSALDEDGRAALRGRMVGFVFQSFQL
                     LPAMTALENVMLPLELAGADDPEPAAREMLGRVGLGDRLHHYPKQLSGGEQQRVAIAR
                     AFVTRPKLLFADEPTGNLDAATGAQVIDLLFELNRESGATLLLVTHDEALTSRCDRVL
                     RLAGGRLQDGET"
     gene            1448..>3354
                     /locus_tag="AC158Q_08015"
     CDS             1448..>3354
                     /locus_tag="AC158Q_08015"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3339762.1"
                     /translation="MNFAREIHLAWRLLSRDYRAGELALIAVAIVIAVASVTTVGFFV
                     SRVQGALDQQANRLMGADLVIADSRPLAQDLRDGAASRGLSVIDILRFPSMAIGGDRS
                     ILAEVKAVTPGYPLRGELRITDRLFAPDRPADAIPDPGDVWVDERLYTSLELAPGGLL
                     TLGNREFRVAAIMTHEPGVEMGFLSGAPRVLLNAGDLASTGLNQPGSRVRYRLNVAGA
                     DEAVEQYREWARGRLQPGQRLEGIRDARPELRSALERAQRFLNLAALTSALLAAVAIA
                     LATRRYLQRHLDGCAVMRCLGAEQSRITRLYVLHFTMLGLLAAATGVAIGALAQLALS
                     AWLGEVVAISLPAAGVLPAIQGMLVGLLLLLGFALPPLMSLAQVPTLRVLRRELGIPK
                     ASGALGYVLGIAVIAGMILWQAQDLRFGFTVLGWLVLAMAAASALGWLLLRALGTIRA
                     RGVSWRFGVASLRRRTLGTILQVVALGLGVMALLTLTLTRGDLLRTWRESLPPNAPNR
                     FIINIQRDQVDPVQKFFQARGLPAPEIFPMVRGRLVRIGDRGVSAEDYTEERPRRLVT
                     REFNLSWASHMQADNRIVEGAWWQGSRARSDQFSVERGLAEALGIRMGDVLTFDIAGT
                     PVTATVTSLRSV"
BASE COUNT          519 a         1085 c         1154 g          596 t
ORIGIN      
        1 gcggtgagcc tcgaattatc gtgtcctagg tgacgcagaa aggaattgcg gcttgtacgc
       61 catgtcgcac cggctcaccg cttatcgctt tcctgatttc ttcagcaaag gccgcaattc
      121 cttccacacc gtatcgagca tcatcatctg gccgttggcg gcggggtgaa agccatcctc
      181 caggaaatac tcccgcttat cggcaaaccc ctcgaacagg aacggcacca gcggcagctt
      241 tttgcgcctg gcgacctcca cgtagatttg ctggaactta tgggagtagt ccgtgccgta
      301 gttgggtggc aggcgcatgc cgaccagcag gacgcgcgcg ccggcgttgc gacaggcgtc
      361 gatgatggcc tccaggttgg cgcgcgtcgc gtcgagggtg gtcccgcgca ggccgtcgtt
      421 cgcgccaagc tccaggatga ctatggatgg gcgatgggcc ttgagcgcgg cttcgatgcg
      481 gttgcggccg ccgtgggtgg tttcaccgct gatgctcgcg ttggctactt tataatcgaa
      541 tttttccgcg gccagacgcc gcgtcagcag gctcacccat ccctgctcct ggggcaggcc
      601 gtaagcagcc gacaggctgt cgccgtaggc cagtatggtc gcggcggaag ccgtcgcggc
      661 cgacagggcc agcgcaacaa ccaacaagac cgcagaaccc gtaaaaatgc tgaatagcct
      721 ggcaccaacc atcataacga cctcggaact cggtaagcag gttaccaccg gcagttccga
      781 attgaccatt ctgagcggcg tgagtttcga agtccgtgcc ggcgaggcgg tcgccatcgt
      841 cggcgtctcg ggatcgggca agtccacgtt gctcggactg ctggcggggc tcgacacgcc
      901 gagcgccggg gccgtgcgga tcgacggtca cgaattgtcc gcgctcgacg aggacgggcg
      961 cgccgcgctg cgcggacgga tggtcggctt cgtgttccag tcattccagc tgctgccggc
     1021 gatgacggcg ctggagaacg tgatgctgcc gctggaactt gccggcgcgg atgacccgga
     1081 gccggcggcg cgggaaatgc tggggcgcgt cggacttggc gaccgccttc accactaccc
     1141 gaagcagctg tcgggcggcg agcagcagcg ggtggcgatc gcgcgcgcgt tcgtgacccg
     1201 gcccaaactg ctgtttgccg acgagccgac cggcaatctc gatgcggcca ccggcgcgca
     1261 ggtgatcgac ctgctgttcg agctgaaccg ggaaagcggc gcgaccttgc tgctcgtgac
     1321 ccacgacgag gcgctgacga gccgctgcga ccgcgtgctg cggctcgccg gtggccgcct
     1381 ccaggacgga gaaacctaga atgaaccgcc aggaccgcca agcacgccaa gattttaatc
     1441 ttatgttatg aatttcgcgc gcgaaattca tctggcgtgg cggctgctct cgcgcgatta
     1501 ccgggcggga gaactcgcgc tgattgcggt ggccatcgtg atcgcagttg cttccgttac
     1561 caccgtgggg ttcttcgtga gccgcgtcca gggggcactc gaccagcagg cgaaccggct
     1621 gatgggggcc gatctcgtga tcgccgactc ccggccgctc gcgcaggatc tcagggacgg
     1681 cgcggccagc cgcgggttgt cggtcatcga catcctgcgt tttcccagca tggcgatcgg
     1741 cggggaccgc agtattctgg ccgaggtcaa ggcggtcacg cccggatacc cgctgcgggg
     1801 cgaattgcgc atcacggacc gcctgttcgc gcccgaccgg ccggctgacg caatacccga
     1861 tccgggcgac gtctgggtgg atgagcggct ttatacctcg ctcgaacttg ccccgggcgg
     1921 cctccttacg ctgggcaacc gcgaattcag ggtggctgcc atcatgaccc acgaaccggg
     1981 ggtggagatg ggttttctct ccggcgcacc gcgggttctg ctgaacgccg gcgatctcgc
     2041 ctccaccgga ctcaaccagc cgggcagccg agtgcgttac cggctgaatg tcgcgggagc
     2101 cgatgaggcg gtcgagcagt accgggagtg ggcgagagga cgattgcagc cgggccagcg
     2161 cctggaaggg atacgcgatg cccgtccgga gctgcgctcc gcgctcgagc gcgcccagcg
     2221 cttcctcaac ctcgcggcgc tgaccagcgc gctgctggcg gcggtggcaa tcgccctcgc
     2281 cacgcggcgc tacctgcagc gccatctcga cggctgcgcg gtcatgcgct gcctgggggc
     2341 ggagcagtca cggattaccc gtctctacgt cctgcatttc accatgctgg gcctgctggc
     2401 cgccgcaacc ggggtcgcga tcggcgccct ggcgcagctc gcgctgtcgg cctggctggg
     2461 ggaagtcgtg gcgatttccc tgccggcagc gggcgtattg cccgccatcc agggcatgct
     2521 ggtcggattg ctgctgctgc tgggctttgc gctgccgcca ctcatgagtc tcgcgcaagt
     2581 gccgacgctg cgcgtgctgc ggcgcgagct gggcatcccg aaggcttcgg gtgccctggg
     2641 ctacgtcctg ggcatcgcgg tcattgcagg catgatcctg tggcaggcgc aagacctgcg
     2701 ttttggcttc acggtcctcg gctggcttgt gctggccatg gccgccgcct ccgcactggg
     2761 ctggctgctg ctgcgcgcgc tggggacgat acgcgcccgg ggagtctcgt ggcgattcgg
     2821 cgtggcgagc ctgcggcgcc gcacgctcgg caccatcttg caggtcgtgg cgctcggtct
     2881 cggcgtcatg gcgctgctca cgctcacgct gacccgcggc gacttgctgc gaacctggcg
     2941 cgaaagcctg ccgccgaatg cgccgaaccg cttcatcatc aatatccaac gcgaccaggt
     3001 cgacccggtt caaaagtttt tccaagcgcg cgggctgccg gctccggaga tattcccgat
     3061 ggtgcgcggg cggctcgtca ggatcggcga ccgcggcgtg tccgcagagg attacaccga
     3121 ggaacgcccg cggcggctcg tcacccggga gttcaacctg tcgtgggcgt cgcacatgca
     3181 ggcagacaac cgcatcgttg aaggtgcgtg gtggcagggc agccgcgcgc gctccgacca
     3241 gttctccgtc gagcgcgggc ttgccgaggc gctgggcatc aggatggggg atgtgctcac
     3301 gtttgatatc gccggaacgc cggtcaccgc cacggtgacg agcctgcgca gcgt
//
LOCUS       DBNGSG010000309         9534 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4005750, whole genome shotgun sequence.
ACCESSION   DBNGSG010000309 DBNGSG010000000
VERSION     DBNGSG010000309.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9534)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9534)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9534
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4005750"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="AC158Q_08020"
     CDS             complement(<1..1059)
                     /locus_tag="AC158Q_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339763.1"
                     /translation="MLSVASAIAQVNGGQGPLPSFRELETAGAVIGEIRISSENIFDL
                     NDPRESSWFYRFGNAIHVPTRVGLLRSLLLFRSGERVSVQLIDETERLLRNQGYLYDV
                     SIRAVAYRNGVVDIEVATRDSWSLDPAIKLTREGGTNTSGLGLKENNFLGTGLALGYA
                     YESDVDRSGNEFSIGHGNAFGSRTVIEYRRADFSDGERDAFRLERPFYELDARWAAGL
                     SATRHGRIDSIYSGGAVSGQYRHQADSGEVYGGLSRGLVNGFAHRVSLGIEYRDDAYR
                     ADPALVAPPELPQDQKLVAPFVRYELVEDDFRKTQNLDRVQRAEYLRMGTNLRLQLGR
                     AMTGLGSTGDWWLYKLEGS"
     gene            1306..2220
                     /locus_tag="AC158Q_08025"
     CDS             1306..2220
                     /locus_tag="AC158Q_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3339764.1"
                     /translation="MTIQATAKKTQVAIMNAVHDLPVLVARDKGFFRDEGLDIEFVTT
                     PGMAQVSTSHSVKFDSVFDRPLDSVYNEGGIDQYRMCEWGIMKRAVEADCSGLRRRKI
                     VALGASMSSFAIAVAADSKIYEPEMLKDKEIAVTPNNGSHFTTLKLMEGFLAQEHIKT
                     TAAGSMPRRIEALKQGKVAAVSLMEPWISVAQKMGLRILIESHSTRSEAAGDDLDGKT
                     LAAMFRAQARAVKELEKDPTPYIHYFIAETGGLLQPHEFQTWRLLHAAPQPYTRERFD
                     DTYNWTVKWNMVVPGATYEKIVDNRAWQ"
     gene            2362..2871
                     /locus_tag="AC158Q_08030"
     CDS             2362..2871
                     /locus_tag="AC158Q_08030"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HIR3339765.1"
                     /translation="MTGDVATGHSGWLSRAAAGLILVALAGCATAPQQTDQDIDVGAD
                     AAQHALGMVGIPYRFGGNSPQGFDCSGLVQYSYARAGMRVSRTVEEQWANSRPVPSEQ
                     IRPGDLLFFHMDGKHNSHIGLYIGNDRFVHAPSTGKHVSVASLANRFWRQHFSIARRP
                     FPDQAEGGL"
     gene            complement(2986..3402)
                     /locus_tag="AC158Q_08035"
     CDS             complement(2986..3402)
                     /locus_tag="AC158Q_08035"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3339766.1"
                     /translation="MRYLVICAALVAGCTAVAPQPETTIPAPAPESVAVAGLMEGARA
                     DATAGRLANAAASLERALRIEPRNPRLWQELARVRLKQGEYAQAESTAARSSSWAGGD
                     NGLRAENWRLIAEARSARGDSRGARAALERSKQLER"
     gene            complement(3399..5663)
                     /gene="mrcB"
                     /locus_tag="AC158Q_08040"
     CDS             complement(3399..5663)
                     /gene="mrcB"
                     /locus_tag="AC158Q_08040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1B"
                     /protein_id="HIR3339767.1"
                     /translation="MPFIRYLKFALLAAALLTAGFALYLDFRVRDEFEGRRFGAPARV
                     YARPLELHVGLRSSQAAVEQELRQLGYRETASGNEPGWFMRRSDGIEIALRPFVFWDG
                     PQPAQRLRLTFDRGRIATLRNAENRDLSLARLDPLLIGGIYPGNNEDRVLVKLEDVPR
                     HLVQALIAIEDRRFYSHAGVDPRGLARAATSLLSGQVQGGSTLTQQLVKNFFLTRERT
                     LRRKFTELIMALLLELHYGKDEILETYLNEIYLGQDRDRAIHGIGLAAQFYFGKPVRQ
                     LTVPEAALLVGMAKGPSYYDPHRQAQRALDRRNLVLREMRNQGHLTMAQYSAARATGL
                     AVSAKPAMGTSPYPAFLQLVYRQLREDYHEADLRSEGLRIFTTLDPRVQGAAERALDR
                     RLVQFDKEKRFGGPGLEGAVVVTDSQNGEVQALVGGRDASYRGYNRALGAARQVGSLL
                     KPAIYLTALSEPSRYTLLTPIDDGPFVWKSRGAKDWRPANYDKRFHGAVPLRVALAQS
                     YNVAAARLGTELGIERVLANVRKLGIQRELRPYASTLLGAVELSPLEVAQLYQTIASG
                     GFRTPLRAIREVLTQDDRPLRRYPLSVEQAFAPEPVYLLTAAMQDVVREGTAQALNNW
                     LPPEMAVAGKTGTTDEQRDSWFAGFTGNRLAVVWVGYDDNRAARLSGATAALPVWGEM
                     MAALDLEPLVLPKPDTIEHVWVDPDTGLRADSGCAGARELPFAEGSAPAEHSPCAGDV
                     PARVRGWLQRLFGR"
     gene            complement(5776..6507)
                     /locus_tag="AC158Q_08045"
     CDS             complement(5776..6507)
                     /locus_tag="AC158Q_08045"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HIR3339768.1"
                     /translation="MEKVTPAALQQMKRDGRKIVGVVAYDYPTAQLADRAGGDLVSVG
                     DSVGANLWGQPTEAEVTIEELLIACKAVRRGTKRALVSCDVPQGPVNAGIEAATRVAA
                     RLAKEGGAEMVKVLGTPELVRAIVRAGVPVFAEFHGENVSVERLVEQARGLEEAGATL
                     LDFRHSGPVAGPAVVKAVSVPVIGGLGGGPWLDGRVRLAHTAIGYGAKWIDSKTETYV
                     NTAKLSLEAFTALCADVRAGRQIKG"
     gene            complement(6523..7383)
                     /locus_tag="AC158Q_08050"
     CDS             complement(6523..7383)
                     /locus_tag="AC158Q_08050"
                     /EC_number="4.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006403946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase family protein"
                     /protein_id="HIR3339769.1"
                     /translation="MALPRLNGIIRALESGQHAFTTFLPPDVESAIAVQASKYDGVVF
                     EGEHNAWDIRVLRDCLQYLLNRAQIAKAGSVPPAVTPTVRIPPNGNEMNQFFAKQALD
                     LGYYGIIWPHVSTVEEAYNAVAACRYPRLKGKPMYEPAGIRGDGPAQAARYWGVTQQE
                     YYERADVWPLNPQGEIFVILLIEDTAGIANLDDMLKNVPGIGAILIGEGDLSQELGYP
                     RQYEHKAVLDAMAQVVATCRKHNVVVGHPHVEAANAARIIGEGYRYLMCSAPRSFATL
                     EKARGLAGRS"
     gene            complement(7482..8519)
                     /locus_tag="AC158Q_08055"
     CDS             complement(7482..8519)
                     /locus_tag="AC158Q_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3339770.1"
                     /translation="MIIDIHGHYTTEPQALHMFRDKQLAGLADAARKPGSTDLGISDE
                     VLVKSVQPQIKFQTERGGDLTIFSPRAGGMAHHVGTEAISRQWTTICNDLIHRICTLL
                     PDNFAPVGQLPQFPGVPPKNCIPELERIVKELGFVGVNLNPDPSGGYWTDPPMTDRYW
                     YPIYEKLCELDVPAMIHVSSSCNPNFHHTGAHYINGDTSVFMQLIQGNLFKDFPTLRL
                     VIPHGGGAVPYHWGRYRGLCQDMGKPPLAEYLLKNVYFDTCVYHLPGIELLAKVIPVD
                     NILFASEMIGAVRGVDPVTGHYYDDTKRYVDQLKGLSEQERHKIFEGNARRVYPRLDA
                     LLKKRGKPKKQ"
     gene            complement(8610..>9534)
                     /locus_tag="AC158Q_08060"
     CDS             complement(8610..>9534)
                     /locus_tag="AC158Q_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR3339771.1"
                     /translation="VAPEGIELNVLELPVEETFFRMARYREFDVSEMSMSSYTVSLNR
                     DPQPFIAIPVFPSRFFRHSSIFVSSKSGIHEPKDLAGRKIGTPEYQMTAPVWIRGILS
                     DDYGVKPDGVEYWTGGEEETQRDEKIKLDLPSKFRVRPIGRDKTLARMIAAGEIDALY
                     TARAPSTFYSKPDAVRRLFPDFVTVERDYYRRTRIFPIMHVVAIRREVYEKNRWIAMS
                     LYKAFAEAQRRTYRSLRETAAHKAMLPWLTAHVEDAVRELGEDWWSYGFQANRHVLDT
                     FLRYHHEQGLSKRRLQPEEMFAPETMEAFKI"
BASE COUNT         1616 a         3195 c         3046 g         1677 t
ORIGIN      
        1 gctgccctcc aatttgtaga gccaccagtc cccggtggag cccaggcccg tcattgcgcg
       61 ccccagttgc aggcgcaggt tggtgcccat ccgcaggtat tccgcgcgct ggacccggtc
      121 gaggttctgg gtcttgcgga aatcatcctc gaccagctca tagcgcacga agggcgcgac
      181 cagcttctgg tcctgcggca gttcgggcgg cgccaccagg gcaggatccg ctcgataggc
      241 gtcgtcgcgg tactcgatgc ccagcgagac gcggtgagca aacccgttga cgaggccgcg
      301 cgagagcccg ccatagactt ccccggagtc ggcctggtgg cgatactgcc cggaaacggc
      361 acccccgctg tagatcgagt cgattcgccc gtgccgcgtc gccgacaggc ccgccgccca
      421 gcgggcatcc agttcgtaaa agggccgctc cagcctgaag gcatcccgct cgccgtcgct
      481 gaaatcggcg cgccggtact cgatcacggt gcggctgccg aaggcattgc cgtggccgat
      541 ggagaactcg tttccgctgc ggtccacgtc ggactcgtac gcgtagccca gggccagccc
      601 cgtgcccagg aaattgttct ccttgaggcc caatccgctg gtattggtgc cgccttcccg
      661 ggtcagcttg atggccggat cgagcgacca cgaatcgcgg gtcgcaacct cgatgtcgac
      721 aacgccgtta cggtaagcga cggctcggat gctgacgtcg tagagatacc cctggttgcg
      781 cagcaggcgc tcggtttcgt cgatcagctg caccgacacc cgctcgccgc tgcggaacag
      841 caagaggctt ctcagcagtc caacgcgcgt cgggacgtgg atcgcgttgc cgaaccggta
      901 gaaccaactg ctctccctcg gatcgttgag gtcgaaaatg ttctcgctgc ttatccggat
      961 ctcaccgatg acggcgccgg cggtttccag ctccctgaac gacggcaggg gaccctgccc
     1021 gccgttgacc tgcgcaatcg cggacgcgac cgacaacaca acggttgcgc aaagtccgca
     1081 tatccgccgg atcaagcgag ccgtcgaggt tcggggagtc accaggcgaa ttatcagccc
     1141 ggaacccggg tgcggcacac atcaatcgtg cgaacgggca tagttcacgg taacccgggc
     1201 cttgatcgcc tcgtcaccct ggagtccctt gtctggcggc cgccccgttg acagccaaaa
     1261 ggacgaagca gaacatgggc gcaacccgct cacccggagg gcgccatgac aatccaggca
     1321 actgcgaaaa aaacccaggt tgcgatcatg aacgcggttc acgacctgcc cgtgctggtc
     1381 gcgcgcgaca aggggttttt cagggacgag ggcctcgata tcgagttcgt gaccacgccg
     1441 gggatggcgc aggtttcgac ctcccattcc gtcaaattcg actccgtttt cgaccgcccg
     1501 ctcgactcgg tttacaacga gggcggcatc gaccagtacc gcatgtgcga atggggaatc
     1561 atgaagcgcg cggtcgaagc cgattgctcg ggcctgcgcc ggcgcaaaat cgtcgcgttg
     1621 ggcgcctcca tgtcgtcgtt cgccatcgcc gttgccgccg actcgaagat ctatgagccc
     1681 gagatgctca aggacaagga gatcgccgtg acgccgaaca acggctccca tttcaccacc
     1741 ttgaagctga tggaaggctt tctcgcgcag gagcacatca agaccacggc agcgggctcg
     1801 atgcccaggc gcatcgaggc gttgaagcag ggcaaggtcg cggcggtgag cctgatggag
     1861 ccctggatca gcgtggcgca gaagatgggc ctgcgcatcc tgatcgagtc gcactcgaca
     1921 cgcagcgagg cggccggcga cgacctcgac ggcaagacgc tcgcggcgat gttccgcgcg
     1981 caggcgcggg ccgtgaagga gctcgaaaag gatcccacgc cctatattca ctatttcatc
     2041 gccgagaccg gcgggctgct gcagccccat gagtttcaga cctggcgcct gctgcacgcc
     2101 gcgccgcagc cctacacgcg ggaacggttc gacgacacct acaactggac ggtgaagtgg
     2161 aacatggtgg tgcccggcgc cacctacgag aagatcgtcg ataaccgcgc ctggcagtaa
     2221 ccttttgagc cggcgtcggc cgccgacagg ggccgggagg gccgcacccg acggtttttg
     2281 acctgtggca cgatcaccgg gaacgccgga acgcgggcgt tgtctttccg gtcatcagcc
     2341 agtgaataca gagcgggtcc aatgaccgga gatgttgcca ccggccactc aggctggtta
     2401 tcgcgcgctg ctgccggact gatcctggtc gcgctcgccg gttgtgcgac cgcaccgcag
     2461 caaaccgatc aggatatcga tgtcggcgcc gatgccgccc agcacgcgct cggcatggtc
     2521 ggcataccgt accgctttgg cggcaactca ccgcagggtt tcgactgcag cggactcgtg
     2581 caatacagct atgcccgcgc cggcatgcgc gtatcgcgaa ccgtggaaga acagtgggcc
     2641 aattcgcgcc ccgtccccag cgagcaaatc cgtccgggcg acctgctgtt ctttcacatg
     2701 gacggcaagc acaactcgca catcgggctc tacatcggca acgaccgctt cgtgcatgcg
     2761 ccttctaccg gcaagcacgt ctcggtcgcg agcctggcca accggttctg gcgccagcat
     2821 ttttccattg cgcggcgccc gtttcccgat caggcggaag gcggattgtg acgggcgatg
     2881 gaaaattgcg ctacagaact caaggcttga accggacctt tgaccgggaa acgggaattg
     2941 ttctatccac gccacactgc cttacgcccg ttccatcatg attacctagc gctcgagctg
     3001 cttcgagcgt tcgagcgctg cccgggcacc ccgggaatca ccgcgtgcgc tgcgcgcttc
     3061 cgcgatcagg cgccagttct cggcgcgcaa gccgttgtcg ccgcccgccc acgaactgga
     3121 gcgcgcggca gtgctctcgg cctgcgcata ttcgccctgt ttcagccgca cgcgcgcaag
     3181 ctcctgccac aggcgcgggt tgcgcggctc gatgcgaagc gcacgctcga gggaggccgc
     3241 ggcgttggcg agccggccgg ccgtggcatc ggcacgcgcg ccttccatca ggccggcgac
     3301 ggcgacgctc tccggggcgg gcgcggggat ggtggtttcg ggctggggcg caacggcggt
     3361 gcaaccggcc accaacgccg cgcagatgac gaggtatctc atcggccgaa cagccgttgc
     3421 aaccagcccc tgacacgcgc gggcacgtcg ccggcgcaag gcgagtgctc tgccggcgca
     3481 gaaccttcgg caaacggcag ttcccgcgcg ccggcgcagc cgctgtcggc gcgcaatccg
     3541 gtatccgggt cgacccacac gtgctcgatg gtgtccggct tgggcaatac cagaggctcg
     3601 agatcgaggg cagccatcat ttcgccccag acaggcagcg cggcggtggc gccgctcagg
     3661 cgtgccgcgc gattgtcgtc gtagccgacc cagacgaccg ccagccggtt gccggtgaag
     3721 ccggcgaacc agctgtcgcg ctgctcgtcg gtcgtgcctg ttttgcctgc gaccgccatc
     3781 tccggcggca gccagttatt aagcgcctgc gccgtgccct cgcgcacgac atcctgcatc
     3841 gcggcggtca atagatacac cggctcgggc gcgaacgcct gttccaccga gagcggatag
     3901 cgcctgagcg gccggtcgtc ctgcgtgagc acttcacgga tcgcgcgcag cggcgtgcga
     3961 aacccgccgc tggcgatcgt ctggtagagc tgcgcgacct cgagcggact cagctcgacc
     4021 gcgcccagca gcgtcgatgc gtacggccgc agttcgcgct ggatgcccag cttgcgcacg
     4081 ttggccagca cccgttctat cccgagctcg gtgccgagcc gcgcggccgc aacgttgtag
     4141 gattgcgcca gcgccacccg cagcggcact gcgccgtgga accgcttgtc gtaattggcc
     4201 ggccgccagt ccttcgcgcc gcggctcttc cagacgaacg gcccgtcatc gatgggagtg
     4261 agcagcgtgt agcgcgacgg ctcgctcaac gcggtgagat agatcgcggg cttcagcagc
     4321 gatccgacct ggcgggcggc gccgagcgcc cggttgtagc cacggtagct cgcgtcacgc
     4381 cctccgacca gcgcctgcac ttcgccgttc tggctgtcgg tcaccacgac cgccccttcg
     4441 agcccgggtc caccgaagcg cttttccttg tcgaactgca caaggcgccg gtccagggcg
     4501 cgctcggcgg ctccctgcac gcgggggtcc agcgtggtga agatccgcag gccttccgag
     4561 cgcaggtccg cttcgtggta atcctcgcgc agctggcggt aaacgagctg caggaaagcc
     4621 gggtacggcg aggtgcccat ggccggcttc gcactgaccg cgagtccggt cgcgcgcgcg
     4681 gcggaatact gtgccatcgt gaggtggccc tgatttcgca tctcgcgcag aacgagatta
     4741 cgccggtcga gcgcgcgctg cgcctggcga tgcgggtcgt agtacgacgg gcccttggcc
     4801 atcccgacca gcagcgccgc ttccgggacc gtaagttgcc gcaccggctt gccgaaataa
     4861 aactgcgccg cgagcccgat gccatggatg gcacggtcgc ggtcctggcc gagatagatc
     4921 tcgttcaggt aggtctccag gatctcgtcc ttgccgtaat gcagctcgag cagcagcgcc
     4981 atgatcagct cggtgaactt gcgccgcagc gtgcgctccc gggtcaggaa gaaattcttg
     5041 acgagttgtt gcgtcaaggt gctgccgccc tgaacctggc ccgacaggag gctcgtcgcc
     5101 gcccgcgcga ggccgcgcgg gtctacgccc gcatgcgaat agaatctccg gtcttctatc
     5161 gcgatcagcg cctgcacgag gtgccgcggc acgtcttcga gcttcaccag cacccggtcc
     5221 tcgttgttgc ccggatagat cccgccgatc agcaaaggat ccaggcgcgc aagggaaaga
     5281 tcccggttct ctgcgttgcg tagtgtcgcg atcctgcccc ggtcgaaagt cagccgcagc
     5341 cgctgcgccg gctggggtcc gtcccagaat acgaaaggcc ggagcgcgat ctcgatcccg
     5401 tcggatctgc gcatgaacca gccgggttcg ttcccgctcg ccgtctcgcg atagccgagt
     5461 tggcgcagct cctgctccac ggccgcctgc gaactgcgca gcccgacgtg caattccagc
     5521 ggccgcgcat aaactcgcgc cggcgcgccg aaacgacgac cctcgaattc gtcgcgcacg
     5581 cggaagtcga ggtacagcgc gaacccggcc gtgagcagcg cggccgcaag cagtgcgaat
     5641 ttgaggtatc gaatgaaagg catcactcgc aattacagac cgccggcggc gcgcttagtt
     5701 cgcgccgggg gccgcgccgc ctggtcgccg tgaagcattt gccgcccgct acccaacgcc
     5761 tcgacgatga gcgtgttacc ccttgatctg ccggccggcg cgcacatcgg cgcacagggc
     5821 cgtgaatgcc tcgaggctca atttggcggt gttcacatag gtttccgtct tcgagtcgat
     5881 ccatttcgcg ccgtagccga tcgccgtgtg cgcgaggcgt acccgcccgt cgagccacgg
     5941 cccgccgccg agcccgccga tcaccggcac ggacacggcc ttaacgaccg ccggcccggc
     6001 aaccggcccc gagtgcctga agtcgagcag cgtggcgccg gcttcttcca gccccctcgc
     6061 ctgctctacg agccgctcga cggaaacgtt ctcaccatga aattcggcaa agaccgggac
     6121 gcccgcgcgc acgatcgccc gtaccagctc gggcgtgccc aggaccttga ccatttcggc
     6181 cccgccctct ttcgcaagcc gggcggcgac ccgggtggca gcctcgatgc ccgcattgac
     6241 cggcccctgc ggcacgtcgc agctcaccag cgcgcgcttc gttccgcgcc gaaccgcctt
     6301 gcacgcgatg agcagctcct cgatcgtgac ttccgcttcc gtcggctgcc cccacaggtt
     6361 cgcacccacg gaatccccga cggacacgag atcccctccg gcgcggtcgg cgagttgcgc
     6421 ggtggggtaa tcgtaggcga ccactccgac gatcttgcgc ccgtcgcgct tcatctgctg
     6481 cagcgcggcc ggggtcacct tctccatatc gggaacaccc ggtcagctac ggcccgccag
     6541 gccacgcgct ttctcgagcg ttgcgaaact gcgcggcgca gagcacatca ggtagcggta
     6601 accttccccg atgatgcgcg cggcgttcgc ggcctccacg tgcggatggc cgacgacgac
     6661 gttgtgcttc ctgcaggtgg cgacgacctg cgccatcgcg tcgagcacgg ccttgtgttc
     6721 gtactggcgc gggtagccga gctcctggct caaatcgccc tcgccgatga ggatggcgcc
     6781 tatgcccggc acgttcttca acatgtcgtc caggttggcg atgccagccg tgtcctcgat
     6841 caggagaatg acgaagatct cgccctgggg attcagcggc cacacgtcgg cgcgctcgta
     6901 gtattcctgc tgcgtgacgc cccagtagcg cgccgcctgc gccggcccgt cgccgcggat
     6961 gcccgccggc tcgtacatcg gcttgccctt gagccgcggg tagcggcagg ccgcgaccgc
     7021 gttataggcc tcctcgacgg tgctgacgtg gggccagatg atgccgtaat aaccgaggtc
     7081 caacgcctgc ttggcgaaga actggttcat ctcgttgccg ttaggcggga tgcgcaccgt
     7141 cggcgtcacc gccggcggca ccgacccggc cttggcgatc tgggcgcggt tcagcaggta
     7201 ctgcaggcag tctcgcagca cccggatgtc ccaggcattg tgctcgccct cgaacaccac
     7261 gccgtcgtac ttcgacgcct gcacggcgat cgccgactcg acgtccggcg gcaggaacgt
     7321 ggtgaacgcg tgctggccgg actcgagcgc gcgaatgatg ccgttcaaac ggggtaatgc
     7381 cattctctct ccttgctgga taaaagtgac gagctgacgc agtgacttag tgacgcagcg
     7441 atcggtctgg cgccctgcct tttcgtcacc tcttcactgc gtcactgctt cttgggcttg
     7501 ccgcgcttct tcagcagcgc gtcgagacgc ggatacacgc ggcgggcatt gccctcgaag
     7561 atcttgtggc gctcctgctc gctcaacccc ttcaactggt cgacgtagcg cttggtgtcg
     7621 tcgtagtagt gcccggtcac ggggtcgact ccccgcaccg cgccgatcat ttcagaggcg
     7681 aacaggatgt tgtccaccgg tatcaccttg gcaagcagct cgatgccggg cagatggtag
     7741 acgcaggtgt cgaaatagac gttcttcagc aggtattccg cgagcggcgg cttgcccatg
     7801 tcctggcaca agccgcgata gcggccccag tgatacggca ccgcgccccc gccatggggg
     7861 atgacgagcc gcagcgtcgg gaaatccttg aagaggttgc cctggatcag ctgcatgaag
     7921 acgctggtgt cgccattgat gtaatgcgcg ccggtgtgat ggaaattcgg gttgcacgat
     7981 gaggacacgt ggatcatcgc cggcacgtcg agctcgcaca gcttttcgta gatcggatac
     8041 cagtaccggt ccgtcatcgg gggatcggtc cagtagccgc ccgagggatc gggattgagg
     8101 ttgacgccga cgaatcccag ctccttcacg atgcgctcga gctcgggaat gcagttcttc
     8161 ggcggcaccc ccgggaactg gggcagctgg ccgaccggcg cgaaattgtc cggcaacagc
     8221 gtgcagatgc gatggatgag gtcgttgcag attgtcgtcc attgccggct gatcgcctcg
     8281 gtgccaacgt ggtgcgccat gccgccggcg cgcggcgaga agatggtgag atctccgccc
     8341 cgctccgtct ggaacttgat ctgcggctgc acgctcttca ccaggacttc gtcgctgatg
     8401 ccgagatcgg tggaacctgg cttgcgcgct gcgtcggcga gccctgccag ctgcttgtcc
     8461 ctgaacatgt gcagcgcctg cggctcggtc gtgtagtggc cgtggatatc aatgatcata
     8521 aatgcgtgat aagcgatgag tgataggtga taggagttta cctgctccgc tgcgcgggag
     8581 tcttatcact tatcacctgt cacttatcac tagattttga aagcttccat cgtttccggc
     8641 gcgaacatct cctcgggctg gagccgccgc ttcgacaacc cctgctcgtg gtggtaccgc
     8701 agaaacgtat cgagcacgtg gcggttggct tgaaagccgt acgaccacca gtcctccccc
     8761 agctcgcgca ccgcgtcctc gacgtgcgcc gtcagccagg gcagcatcgc cttgtgcgcg
     8821 gcggtctcgc gcaggctgcg gtaggtgcgg cgctgcgcct cggcgaaggc cttgtagagc
     8881 gacatcgcga tccagcggtt cttttcatag acctcgcggc ggatcgccac cacgtgcatg
     8941 atcgggaaga tcctcgtgcg ccggtagtag tcccgttcca ccgtgacgaa atccgggaac
     9001 agccgcctga ccgcatcggg cttcgagtaa aaagtcgagg gcgcgcgcgc cgtgtagagc
     9061 gcgtcgatct cgcccgctgc gatcatcctt gccagcgtct tgtcgcggcc gatcgggcgc
     9121 accctgaatt tggaagggag atcgagcttg atcttttcgt cgcgctgagt ctcctcttcc
     9181 cctccggtcc agtactccac gccgtcgggc ttgacgccgt aatcatcgga caggatgccg
     9241 cggatccaca ccggcgcggt catctggtac tcgggggtgc cgatcttcct gcccgccagg
     9301 tccttcggct cgtggatgcc gctcttcgag gagacgaaga tgctggaatg acggaaaaag
     9361 cgcgacggga ataccgggat ggcgatgaac ggctgcggat ctcggttaag cgaaaccgtg
     9421 tacgacgaca tcgacatctc ggagacgtcg aattcccggt agcgcgccat ccggaagaag
     9481 gtctcttcga ccggcagctc cagcacgttg agctcaatgc cttccggcgc gacc
//
LOCUS       DBNGSG010000310         1353 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_100810, whole genome shotgun sequence.
ACCESSION   DBNGSG010000310 DBNGSG010000000
VERSION     DBNGSG010000310.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_100810"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="AC158Q_08065"
     CDS             <1..712
                     /locus_tag="AC158Q_08065"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HIR3339772.1"
                     /translation="ETKNWGPDKLYYAARSELDSKNYQKAIGLYQKLESRYPYGRFAQ
                     QAQLDIAYAYWKDTEPALALAACDRFIREHPNHPNVDYAYYLKGRVNFNEDLGILGFI
                     SRKDLTERDPLAARDAFEAFKVLIQRFPDSKYASDARARMLFLVNALSAHEANVAEYY
                     LRRGAYVAAVNRAQSVLAHYPQTPAVERALVVLVKGYEAMGIERLRDDSLRTLKANFP
                     ENTLAVKNGRDRAWWMFW"
     gene            complement(749..1051)
                     /locus_tag="AC158Q_08070"
     CDS             complement(749..1051)
                     /locus_tag="AC158Q_08070"
                     /inference="COORDINATES: protein motif:HMM:NF014624.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HIR3339773.1"
                     /translation="MPATKRSNLKPARLERDKTGLLKRLNRIEGQVRGVNRMVDEGRY
                     CVDILTQISAIRSALDAVALELLRDHTHGCVQDAVRSGSGTAAIDELLTVVEKFAH"
     gene            complement(1029..1235)
                     /locus_tag="AC158Q_08075"
     CDS             complement(1029..1235)
                     /locus_tag="AC158Q_08075"
                     /inference="COORDINATES: protein motif:HMM:NF012621.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HIR3339774.1"
                     /translation="METAVLKVSGMTCGGCVKSVTNVLRSVAGVDAAEVSLERGEARV
                     SFDPAKASLSQLKEAIEDAGYQTQ"
BASE COUNT          246 a          441 c          410 g          256 t
ORIGIN      
        1 cgagaccaag aactgggggc ccgacaagct ctattacgcc gcgcgctcgg agctcgacag
       61 caagaactat cagaaagcga tcggcctgta tcagaagctg gagtcgcgct acccctacgg
      121 ccgcttcgcg caacaggccc agctcgacat tgcatacgct tactggaagg acaccgagcc
      181 ggcactggcg ctcgcggcct gcgaccgctt catccgcgag catcccaatc atcccaacgt
      241 cgactacgcc tattacctga agggccgggt caacttcaac gaggatctcg gcatactcgg
      301 gttcatcagc cgcaaggacc tgacagagcg cgacccgctc gcagcccggg acgccttcga
      361 ggcgttcaag gtgctgatcc aacgttttcc ggacagcaag tacgcttccg acgcgcgtgc
      421 caggatgctg ttcctcgtca acgcgctgtc ggcgcacgag gcgaacgtcg ccgagtacta
      481 tctgcggcgc ggcgcttacg tggcggcggt taatcgcgcg cagagcgtgc tggcccacta
      541 tccgcagacg ccggccgtcg agcgggcgct ggtggtgctg gtcaagggct atgaggcaat
      601 ggggatcgaa cggttgcgcg acgacagcct gcgcacgctc aaggcgaatt ttcccgagaa
      661 cacgcttgcc gtgaagaatg ggcgcgatcg cgcctggtgg atgttctggt agcgatccgg
      721 gtgggccgat ttgcccgcgc accgggaatc agtgggcaaa tttttccacg acggtcagca
      781 gttcgtcgat tgccgcggtt ccgctgccgg agcgcaccgc atcctgcacg cagccatggg
      841 tatgatccct gagcagctcg agcgcgacgg cgtcgagggc ggaacggatc gccgagatct
      901 gggtgagtat gtcgacgcaa tagcggcctt cgtcgaccat ccgattcacg ccgcggacct
      961 ggccttcgat gcgattgagg cgcttgagaa ggccggtctt gtcgcgctcg agccgcgcgg
     1021 gtttgaggtt actgcgtttg gtagccggca tcttcgatcg cctccttgag ttgcgacaga
     1081 ctggctttcg cgggatcaaa actcactcgc gcctcgccgc gctccagaga aacttcggcg
     1141 gcgtccacgc ccgccaccga cctgagcaca ttggtcacgc ttttcacgca accgccgcag
     1201 gtcataccgc ttactttcag taccgccgtt tccatccgtt ctccttcaat aattccaacc
     1261 gcagaggcct acctcacccc ccgccctttc cactgcgtgg agagggagcc gacgtggctg
     1321 gattcttgtg ggtttctgac accccctccg ccc
//
LOCUS       DBNGSG010000311         3501 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3836268, whole genome shotgun sequence.
ACCESSION   DBNGSG010000311 DBNGSG010000000
VERSION     DBNGSG010000311.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3836268"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="AC158Q_08080"
     CDS             complement(<1..433)
                     /locus_tag="AC158Q_08080"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3339775.1"
                     /translation="MKALIVAIIAALYVIPARDAGAQAYPAKPIRLIVPFAPGGPNDV
                     IGRVVAQKVSELIGQPIVIDNRTGAGGAVGTAAAGTSAPDGYTLLISGTSSLAINPSL
                     YKKLPYDPLRDFAPVSLVGTAPSLLAMHPSVPVRSVKDLIAL"
     gene            556..1047
                     /locus_tag="AC158Q_08085"
     CDS             556..1047
                     /locus_tag="AC158Q_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HIR3339776.1"
                     /translation="MPSFDIVSEVNHVEIRNAVDQANKEITNRFDFKGSDARVEINEK
                     EKTLAAYADDEFKLRQVRDVLTGKLAKRGVDIRALEPGKTEAVSGNKVKETIKVREGI
                     EQERAKGIVKLIKDSKLKVQGSIQGDLVRVSGAKKDTLQEAIALLRKSVTDIPLQFKN
                     FRD"
     gene            complement(1048..1764)
                     /locus_tag="AC158Q_08090"
     CDS             complement(1048..1764)
                     /locus_tag="AC158Q_08090"
                     /inference="COORDINATES: protein motif:HMM:NF012345.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HIR3339777.1"
                     /translation="MKPVAIFRHAASEGPGYFATYLDRHGVPWRVIRIDEAEAVPENP
                     LEFSGLAFMGGPMSVNDDLPWIASELRLIRSAVAGGVPVLGHCLGGQLIAKALGGVVA
                     RNPVREIGWGRVDVLPNDTARAWFGTGPLSFESFHWHGEAFSIPPGAVRLLSSPYCEN
                     QAFALGPHFGMQCHVEMTPELIHAWCREWGKEVETLARRVPSVQTPAQMTQRVEERAA
                     RLNAIADRLYDRWTEGLRRS"
     gene            1803..2444
                     /locus_tag="AC158Q_08095"
     CDS             1803..2444
                     /locus_tag="AC158Q_08095"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease family
                     protein"
                     /protein_id="HIR3339778.1"
                     /translation="MPTILAGLVLFLAHAFAAAADINVIALTAGKAVVTINNGKPLTM
                     ATGQVTPEGVRMIGATTESATFEINGKRQTLTMGQSISIGGGPVSAQRATLIADTGGH
                     FVTVVAVNGVSMRFMVDTGASLVTLSSGDAKRAGINYLSGQKAVLQTANGTTAAFRVK
                     LDTVRLGDITLNDVDGVVVEGNVMGELGLLGLSFLNRLNMRREGSTMTLTRRF"
     gene            complement(2455..3123)
                     /gene="ribA"
                     /locus_tag="AC158Q_08100"
     CDS             complement(2455..3123)
                     /gene="ribA"
                     /locus_tag="AC158Q_08100"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006766158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HIR3339779.1"
                     /translation="MTGKKTSKSGTDVRLLAETTLPTRKAGAFRLLVYGDSMDNNVLL
                     ALVKGQVKSGEPTLVRLHSQCLTGDVFGSERCDCGEQLDTALALIEKQGAGVLVYMFD
                     EGRGIGLLNKIRAYALQDQGRDTVEANHALGFAADMRDYRTGAHILLDLGARKVRLMT
                     NNPDKVRQLEQYGLTVTERIPLEIAPGKSNRDYLRTKRTKFGHLLSLVPEQQAAAPAP
                     GKKR"
     gene            complement(3150..>3501)
                     /gene="folE"
                     /locus_tag="AC158Q_08105"
     CDS             complement(3150..>3501)
                     /gene="folE"
                     /locus_tag="AC158Q_08105"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HIR3339780.1"
                     /translation="DIELYSLCEHHVLPFFGKCHVAYIPKGRIVGLSKIPRVVDAFAR
                     RLQVQERLTVDIRNCIEETLKPQGVAVVIEAQHMCMVMRGIQKQHSIATTSAFTGEFN
                     TDKTRAEFLRLIGR"
BASE COUNT          645 a         1123 c         1132 g          601 t
ORIGIN      
        1 cgagcgcgat caggtccttc accgatctca ccggcaccga tggatgcatg gcgagcaggc
       61 tcggcgcggt gcccaccagg ctgaccggcg cgaaatccct gagcgggtcg tagggcagct
      121 tcttgtacag gctcggattg atcgccagcg aagaggtgcc cgagatcagc agcgtgtagc
      181 cgtccggggc cgacgtgccg gcggcagcgg tgccaaccgc gccgccggcg ccggtacggt
      241 tgtcgatgac gatcggctgt ccgatcagct cactcacttt ctgcgccaca acgcgcccga
      301 tcacgtcgtt gggaccgccg ggcgcaaacg gcacgatcaa gcgtatcggt tttgcgggat
      361 aggcctgtgc gccggcgtcg cgcgcgggaa tcacgtatag cgctgcaatg attgccacga
      421 tcaatgcttt catggaagtt cctcccgggc cgattatgca tcaatccttc aaacgacgag
      481 gaaaccgtaa accgggggcc tcatatccgg cttgcttcat ggggtagaat tgcagcggtg
      541 ttggaaggag gactcatgcc gtctttcgat atcgtctccg aagtgaacca cgtcgagatc
      601 cgcaacgccg tggaccaggc gaacaaggaa atcaccaacc gtttcgactt caagggttcc
      661 gacgcgcgcg tcgaaatcaa tgaaaaggag aagacgctgg cggcgtacgc cgacgacgag
      721 ttcaagctgc gccaggtgcg cgatgtcctc accggcaagc tcgccaagcg gggcgttgac
      781 atccgcgccc tggagcccgg gaaaacggag gcggtgagcg gcaacaaggt caaggaaacg
      841 atcaaggtgc gcgaaggcat cgagcaggag cgcgccaagg gtatcgtgaa gctcatcaag
      901 gacagcaagc tgaaggtcca gggcagcatc cagggcgacc tggtgcgcgt ttccggcgcc
      961 aagaaggaca ccctgcagga agcaatcgcg ctgctgcgga agtcggtcac cgacatcccg
     1021 ctgcagttca agaatttccg ggactgatca ggatctccgc agcccctccg tccagcggtc
     1081 gtagaggcga tcggcgatcg cgttaagccg cgccgcccgt tcctcgacgc gctgcgtcat
     1141 ctgcgcgggc gtctgcaccg agggcacgcg gcgcgcgagc gtctcgactt cctttcccca
     1201 ctcccggcac caggcgtgaa tcagctccgg cgtcatctcg acgtggcact gcatgccgaa
     1261 atgcggcccc agcgcgaagg cctggttctc gcaatacggg ctcgacagca ggcgcaccgc
     1321 gcccggcggg atggaaaacg cttctccgtg ccaatgaaat gattcgaacg acagtggccc
     1381 ggtgccgaac caggcccggg cagtgtcatt gggcagcaca tcgaccctgc cccagccgat
     1441 ttccctgacc gggttgcgcg ccaccacccc gcccagcgcc ttcgcgatca gctggccgcc
     1501 gaggcaatgg ccgagcaccg gcacgccgcc ggcaaccgcg gagcggatca gccgcagctc
     1561 cgaggcgatc cacggcaggt cgtcgttcac gctcatcggc ccgcccatga acgcgagccc
     1621 gctgaattcg agcgggtttt ccgggactgc ttcggcctcg tctatcctga tcacccgcca
     1681 ggggacaccg tggcggtcca ggtatgtggc aaaataaccg gggccttcgg aggcggcgtg
     1741 acggaatatc gctacgggtt tcactgtttg ccctgaaagt ccctaaggac ggaaactcgg
     1801 caatgccaac cattctagcc ggtcttgttc ttttcctcgc gcacgcgttc gccgcggcgg
     1861 cggacatcaa tgtgatcgcg ctgacggccg ggaaagcggt ggttaccatc aacaacggca
     1921 agccgctgac gatggcgacg ggacaggtta cccccgaggg cgtcagaatg atcggcgcca
     1981 ccacggaatc ggccacgttc gagatcaacg gcaagcgcca gacgctgacc atggggcaaa
     2041 gcatatccat cggcggcggc cccgtcagcg cccagcgggc gaccctcatc gcggacaccg
     2101 gcggtcattt tgtgaccgtc gtggccgtca acggcgtttc catgaggttc atggtggata
     2161 ccggcgcctc gttggtcacc ctcagcagcg gtgacgccaa gcgcgccggg atcaattacc
     2221 tgtcggggca gaaagcggtg ctgcagaccg cgaacggcac cacagcggcc ttccgcgtca
     2281 agctcgacac cgtgcggctt ggcgatatca cgttgaacga cgtggacggc gtggtggtcg
     2341 agggcaacgt catgggagaa ctgggcctcc tcggcctgag ctttctcaat cgcctcaaca
     2401 tgcggcggga aggctcgacg atgaccctca cccgccgctt ctagggaagc ggcgtcaacg
     2461 cttcttgccc ggcgcgggtg ctgcggcctg ctgttcggga acgagcgaca gcaggtggcc
     2521 gaacttggtg cgcttggtgc gcaggtaatc gcggttggac ttgccgggcg ctatttcgag
     2581 cggaatgcgc tcggtgacgg tcaggccgta ctgctcgagc tggcgcacct tgtccgggtt
     2641 gttggtcatg agccggacct tgcgcgcgcc gagatcgagc aggatgtgcg cgccagtcct
     2701 gtaatcgcgc atgtcggcgg cgaaaccgag cgcgtggttg gcctcgaccg tgtcgcggcc
     2761 ctggtcctgc agggcgtagg cgcggatctt gttgagcagc ccgataccgc ggccttcgtc
     2821 gaacatgtag accagcacgc cggctccctg cttctcgatc agcgcgagcg ccgtatcgag
     2881 ttgttccccg caatcgcaac gctcgctgcc gaagacgtca ccggtcaggc actgcgagtg
     2941 cagccgcacc agcgtcggct ccccggactt gacctgcccc ttcacgaggg caagcagcac
     3001 gttgttgtcc atgctgtcgc cgtacacgag caaccggaaa gcgccggcct tgcgcgtggg
     3061 cagcgtcgtt tcggcaagca ggcgaacgtc cgtgccggac ttgctggtct tcttgcccgt
     3121 cacgggtcac ccatcgcgcg gcgcgggcat cagcgcccga tcaatcgcag gaactcggcc
     3181 cgcgtcttgt cggtgttgaa ctcgccggtg aatgccgaag tggtcgcgat cgagtgctgc
     3241 ttctggatgc cgcgcatcac catgcacatg tgctgcgcct cgatcaccac cgccacgccc
     3301 tgcggcttca gggtctcttc gatgcagttg cggatatcga ccgtcaggcg ttcctgcacc
     3361 tggagccgcc gcgcgaaagc atccactact cgcggaatct tggacaagcc cacgatgcgg
     3421 cccttgggga tgtaggcgac gtggcacttg ccgaagaacg gcaggacatg gtgctcgcac
     3481 agcgagtaga gctcgatgtc c
//
LOCUS       DBNGSG010000312         7314 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1969706, whole genome shotgun sequence.
ACCESSION   DBNGSG010000312 DBNGSG010000000
VERSION     DBNGSG010000312.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7314)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7314)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7314
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1969706"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            324..1901
                     /locus_tag="AC158Q_08110"
     CDS             324..1901
                     /locus_tag="AC158Q_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HIR3339781.1"
                     /translation="MPEHQVQNWHVRKPLARSRGGIVASQNRVAAQAGAQVLAAGGNA
                     VDAAVATGFALAAVEPWNSGLGGIGFMLVYSAKQRRVSVVDFGPVSPAGLDPAAYPLT
                     GAGSTTRDLFTWPAVKDDRNVHGPLSIAVPGHPDGLGLALEQFGTMKLADVMKPAIAF
                     AEQGIAVDWFLTLKVATMAKELARYPVTRDIWLPNGFPPVTAAGAALERLKLKGLAAT
                     LKRLAGAGRRDFYEGEIASSIVKDVKAMGGVLGTEDLRGYRARVVEPLMAAHNGAQFA
                     LAPGLTAGPSMLRALGALRGVRFRRNGPHADAFVAYARVLRQAYTERLETMGETADRR
                     SPSTTTHLNVVDRHGNMVALTQTLLSVFGSKVVLPSTGILMNNGIMWFDPRLGMPNSL
                     APAKRPLTNMCPVIALRGGRAWFAIGASGGRKILPAVLQISSFLVDHGLDLDEAFHQP
                     RIDASEVEYVALDPRLPASVHKALAKQFPTRSNELVVYPTNYACPSAVQRDAKSRENS
                     GMTDVMSPWSGAVAEGE"
     gene            2120..3130
                     /locus_tag="AC158Q_08115"
     CDS             2120..3130
                     /locus_tag="AC158Q_08115"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3339782.1"
                     /translation="MINAAIIGLGRWGQNLVNSVHGKSDAIRFVAGVVRDPVKTQAYA
                     RDKGFPLYDSYGKVLADPKVDAVVLATPHTRHAEQIVAAAKAGKHVFTEKPLALTAKS
                     AATALRACAKARVTLGVGYNWRFQPALQEIRRMLEDGRLGKLLHVEGNFCGPSAYRFP
                     KGHWRQAREEGPAGGMTGRGVHVVDAFLYLAGRVESVHAQSARRVLRYGIDDTTSMLF
                     RFRGGASGYLGTVIATAETWRMQVFGSKGWVEVGDVEHLTTWEMKTCFVDPQNLLVHH
                     KPQVKRFDAVSTERAELEYFARAAAARRPLAVAGGDEVHGVSVFEAILKSAKARKTVR
                     LR"
     gene            complement(3160..3735)
                     /locus_tag="AC158Q_08120"
     CDS             complement(3160..3735)
                     /locus_tag="AC158Q_08120"
                     /inference="COORDINATES: protein motif:HMM:NF016269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HIR3339783.1"
                     /translation="MITRLLMGGVLVAAMALPYGQAVAQGKDDRQAKRAEVQKMASDT
                     LAQLYKAKPEAKGKIEKAAGYGVFSNFGITIVFLGGAGGKGMVHDNASKKVTYMNMGQ
                     AQAGLGLGATKYKAVFIFKDAKTLQTFVDKGLELGGQGGVAAKAGKAGAADTAGTSIH
                     PGIEIIQLSETGAIVGVTLAGTKYWKDGDLN"
     gene            complement(3871..5106)
                     /locus_tag="AC158Q_08125"
     CDS             complement(3871..5106)
                     /locus_tag="AC158Q_08125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3339784.1"
                     /translation="MKKVTIIGAGIVGMATAAYLRRDGFDVTVVTEHPPGEYCSFGNA
                     GMLNNAGCVPQAMPGVVWKVPGYLSDPLGPLVVRWPYLAQSMPWMLRFLGNANRKQAE
                     HASKALYSLIKDTVDTYEKLLLIAGSTDLVRRSGYLVAYDSERSYQDDALAWQLRRDR
                     GVKMEVLDAAGIRALVPQLAPAYLRGVHVLDQGYVTNPERLVKSLAAWFQKDGGTILH
                     RKVTGIETDAGGARKLVTPSGRLPLETLVVCAGVHSGELSAMLGEPVPIEAERGYHVT
                     YSDPGLRLPLPVHVSDAKVFATPMEMGVRVAGQAEFAGIYAEPNYQRAEVLETHMRRM
                     FPQVRTADSTRWMGRRPSMPDSLPVIGKSARHRNVYYAFGHGHLGLCGGAPTGKIIAA
                     VVSGRKSPIDLEPFRVDRF"
     gene            complement(5227..5301)
                     /locus_tag="AC158Q_08130"
     tRNA            complement(5227..5301)
                     /locus_tag="AC158Q_08130"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5267..5269),aa:Val,seq:cac)
     gene            5466..6311
                     /gene="nudC"
                     /locus_tag="AC158Q_08135"
     CDS             5466..6311
                     /gene="nudC"
                     /locus_tag="AC158Q_08135"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HIR3339785.1"
                     /translation="MADDYIPLYQPPEARSEPALWFAFRKSEILVVSGADRPDLPCCM
                     ELGEHGLQPQRSQYLGLYGGRHCYAAEIHESLPLPAGWAALGLRDLFGLVETTLAALS
                     GRAYQILEWDRNHQYCSRCGTPTRLRSDERSRTCPACQYTTYPPVTPAIMILVTHGRK
                     LLLARKPAFPKGRFSALAGFVEPGEMLEDTVIRETREEVGVEIRNIRYFGSQPWPFPH
                     SLMVAFTAEYAGGEVRPDGVEIEEAQWFEAGELPKLPPSISISRRLIDTVAGRLARGE
                     PASGA"
     gene            complement(6313..7023)
                     /gene="dnaQ"
                     /locus_tag="AC158Q_08140"
     CDS             complement(6313..7023)
                     /gene="dnaQ"
                     /locus_tag="AC158Q_08140"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011656490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HIR3339786.1"
                     /translation="MRQIILDTETTGLEPELGHRVIEVAGVELVNRRFTGKDFHRYFN
                     PGRDSDAAALEIHGLTTEFLADKPKFTDVAAEFLDFIKDAELVIHNSAFDIAFLNREL
                     DLADLKPVTEYCSGVVDTLRMARELHPGKRNGLDALCERYQIDNSARTLHGALLDARL
                     LAEVYLSMTRGQESLMMEVEAPVTAESAAAAEIATGKLNLIIVSATPAELEAHARQLE
                     AIDKASKGATLWRKLEKA"
     gene            complement(7034..>7314)
                     /locus_tag="AC158Q_08145"
     CDS             complement(7034..>7314)
                     /locus_tag="AC158Q_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006758689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNase H family protein"
                     /protein_id="HIR3339787.1"
                     /translation="ETLEPGTRARVHTDSQYVQLGIKEWIHSWKRRGWRTADRKPVKN
                     DDLWRQLDALREKHEIEWLWVRGHDGNPGNEQADALANQGVESVMNKR"
BASE COUNT         1254 a         2433 c         2309 g         1318 t
ORIGIN      
        1 tcttcatgag gtgttccgga tactgcgtcg cagatacaac gtctcaggta cggcgacccg
       61 gatagagcgt gaagttgcgc attacctgga acgacctatc tgagacgctc cacctgagac
      121 gcatcacctg agacgcctta ccaggcacgc tcacccgtta ccccttttcc gttatgattc
      181 ggcgtgatta tatcgttcgc cttcccattc ccctagtgcc gttccaacgt attccgacca
      241 atccatgatg cgcgatcgag gcacaatatt cagtagaagg atcctcgtgg ttatcaggcc
      301 aaccaggttg gaacggcact agcatgccgg agcaccaagt acagaactgg cacgttcgca
      361 agccattggc gcgctcgcgc ggcggcatcg tggcgagcca gaaccgtgtc gccgcacagg
      421 caggcgcgca agtgcttgcg gcgggcggca acgcggtaga cgcggcggtg gcgacaggct
      481 tcgcgcttgc cgctgtggaa ccctggaaca gcggacttgg cggcatcggc ttcatgctgg
      541 tctattcggc gaagcagcgg cgcgtatccg tcgtggactt cggcccggtc tcgcccgccg
      601 ggctcgaccc cgcggcctat cccttgaccg gcgcgggcag caccacgcgc gatctcttca
      661 cctggccggc ggtcaaggac gaccgcaacg tgcacgggcc gctctcgatc gccgtgcccg
      721 gccatcccga cgggctcggg ctcgcgctcg agcaattcgg cacgatgaag ctcgccgacg
      781 tcatgaagcc ggcgatcgcg ttcgcggaac aaggcatcgc cgtggactgg tttctcacgc
      841 tcaaggtcgc caccatggcg aaggagctcg cgcgctaccc ggtcacgcgc gatatctggc
      901 tgccgaacgg ctttccgccg gtcaccgccg cgggcgccgc cctggaacgc ctgaagctca
      961 agggcctcgc cgccacgctc aagcgactcg ccggcgccgg ccgccgtgat ttctacgagg
     1021 gcgaaatcgc atcctccatc gtcaaggacg tcaaggcgat gggaggcgtc ctcggcaccg
     1081 aagacctgcg cggctaccgc gcacgcgttg tcgaaccgct gatggccgcc cataacggcg
     1141 cgcaattcgc gctcgcgccg gggctcaccg ccggccccag catgctgcgg gcattgggcg
     1201 cgctgcgcgg cgtgcgcttc cgccgcaacg ggccgcatgc cgacgctttc gtcgcctacg
     1261 ccagggtatt gcgccaggcg tacaccgaac ggctcgagac catgggcgag accgcagacc
     1321 ggcgctcccc ctccaccacc acgcacctca acgtggtgga ccgccacggc aacatggtgg
     1381 cgctcacgca aacgctgctc tcggtgttcg gctccaaggt ggtgctgccc tcgaccggaa
     1441 tcctgatgaa caacggcatc atgtggttcg acccccgcct gggcatgccc aacagccttg
     1501 cgccggccaa gcgccctctc accaacatgt gcccggtcat cgcgctgcgc ggcggcaggg
     1561 cctggttcgc gatcggcgcc tccggcggcc ggaagattct cccggcggtg ctgcagattt
     1621 catccttcct ggtggaccac gggctcgacc tcgacgaagc cttccaccag ccgcgcatcg
     1681 acgcgagcga ggtggaatac gtggcgctcg acccacgcct gcccgcctcc gtgcacaagg
     1741 cgctggcaaa gcagtttccg acgcggtcga acgagctggt ggtgtatccc accaactatg
     1801 cctgcccgag cgcggtgcag cgcgacgcga agagccgcga gaactccggc atgacggatg
     1861 tcatgtcgcc gtggtccggc gcggtagcag agggcgaata gacaggatta actgaactcg
     1921 atcgaaagac ggcaaacccg ccaaaaccgt aaatggacag gattaacaag atttacagga
     1981 ttagaccaaa aacaaaaccg ggaatgatct gttgtttgaa tcttgttaat cctgtaaatc
     2041 ctgtctatta atgggtttta cgggtttttg aaatactctg tacatcctgt ctattgatga
     2101 atttgacggg ttcttgaaaa tgatcaatgc cgccatcatc ggcctcggcc gctggggaca
     2161 gaacctcgtc aacagcgtgc acggaaaaag cgacgcaatc cgcttcgtcg cgggcgtggt
     2221 ccgcgacccc gtaaagactc aagcctacgc ccgtgacaag ggtttcccgc tctacgactc
     2281 gtacgggaaa gtgctcgccg acccgaaggt ggacgccgtg gtgctcgcca cgccgcatac
     2341 ccgccatgcg gagcagatcg ttgccgccgc gaaggcgggc aagcacgtct tcaccgaaaa
     2401 accgctcgcg ctgacggcga agagcgcggc cacggcgctg cgcgcctgcg cgaaagcgcg
     2461 cgtcaccctc ggcgtcggct acaactggcg tttccagcct gccctgcagg agatccggcg
     2521 catgctggag gacggccgcc tcggcaaact gctccacgtg gaaggcaatt tctgcgggcc
     2581 cagcgcctac cgcttcccga agggccactg gcgccaggcg cgcgaggaag gcccggcggg
     2641 cggcatgacc ggtcgcggcg tgcacgtggt ggacgctttc ctctatctcg ccggacgcgt
     2701 cgaatcggta cacgcgcaga gcgcccgccg cgtgctccgt tacggcatcg acgacacgac
     2761 ctcgatgctg ttccgcttcc gcggcggcgc cagcggctac ctcggcaccg tgatcgccac
     2821 cgcggaaacc tggcgcatgc aggtgttcgg atcgaaaggc tgggtcgagg tgggcgacgt
     2881 ggagcacctg acgacgtggg aaatgaaaac ctgcttcgtc gacccgcaga acttattggt
     2941 gcatcacaag ccgcaggtca agcgcttcga cgccgtcagc accgagcgcg cggaactgga
     3001 atacttcgcc cgcgccgccg cggcgcgccg cccgctcgcc gtcgccggcg gcgatgaagt
     3061 gcacggcgtc tcggtcttcg aggctatcct gaagtcggca aaggcacgga aaaccgtccg
     3121 ccttcgctga cgcctcacac cgttcccggc ccgcccgggt cagttgagat cgccgtcctt
     3181 ccagtacttg gtgcccgcga gcgtaacgcc gacgatcgca ccggtctccg agagctggat
     3241 gatctcgatc cccggatgta tcgacgtgcc cgccgtatcg gctgcgccgg ccttgcccgc
     3301 cttcgccgcg acaccccctt gcccgcccag ttccaggccc ttgtcgacga aggtctgaag
     3361 agtcttggcg tccttgaaga tgaacacggc cttgtatttc gtggcgccga ggccgagccc
     3421 ggcctgcgcc tgccccatgt tcatataggt aaccttcttc gatgcgttat cgtggaccat
     3481 gcccttgccg ccggcacccc ccaggaacac gatggtgata ccgaagttgc tgaatacacc
     3541 gtaacccgcc gccttctcga tcttgccctt ggcttccggc ttcgccttgt agagttgggc
     3601 cagggtatcg gatgccatct tctgaacttc cgcgcgcttc gcttgcctgt cgtccttgcc
     3661 ttgcgcaaca gcctgaccgt acggcaaggc cattgcggcg acgagaacgc cacccatcag
     3721 cagtcgagta atcatgtgga ctcctttggt ttcttgcggc ccggggccgc ggcctcgaac
     3781 tggcgccgga tattgattac ccggccaaca gtgcgaatcc gctgcgaggc aactacggtc
     3841 aggaaacagc aagctgccgg cagctagctg ttagaagcga tccaccctga aaggttcaag
     3901 gtctatcggc gactttctcc cggacacgac agcggcgatg atcttcccgg tgggcgcgcc
     3961 gccgcacagg cccaggtgcc cgtggccgaa agcgtaatag acgttgcggt gtctggctga
     4021 tttcccgatc accggcagcg aatcgggcat ggatgggcgc cggcccatcc agcgcgtgct
     4081 gtcggcggtg cgcacctgcg ggaacatgcg ccgcatgtgg gtttcgagca cctccgcccg
     4141 ctggtaattg ggctcggcat agatgccggc gaattccgcc tgccccgcga cgcgcacgcc
     4201 catctccatc ggcgtcgcga ataccttggc gtccgacacg tgaaccggca gcggcagccg
     4261 cagccccgga tcggagtagg tgacgtggta gccgcgctcc gcctcgatcg gcaccggctc
     4321 gcccagcatt gccgagagct ccccggaatg cacgccggca cagacaacca gcgtctccag
     4381 cggcagccgg ccggatggcg ttacaagctt gcgggcgccg ccggcatcgg tctcgatgcc
     4441 ggtgaccttg cgatgcagga tcgtgccgcc gtctttctgg aaccaggcgg cgagcgattt
     4501 caccagccgc tccggattcg tgacgtagcc ctggtcgagc acgtggaccc cgcgcaggta
     4561 agccggcgca agctgcggca ccagggcgcg aatgcccgcg gcgtcgagca cctccatctt
     4621 cacgccccgg tcgcggcgca gctgccaggc gagcgcgtcg tcctggtagc tccgctcgct
     4681 gtcgtaggcc accaggtagc cgctgcggcg cacgaggtcg gtcgagcccg cgatcagcag
     4741 cagcttctcg taggtgtcca ccgtgtcctt gatcagcgag tacagcgcct tcgaggcatg
     4801 ctccgcctgc ttcctgtttg cgttgccgag aaagcgcagc atccacggca tcgattgggc
     4861 caggtacggc cagcgcacca ccagcggccc caggggatcg ctgagatagc cgggcacctt
     4921 ccagaccacg ccgggcatgg cctgcggcac gcagcctgcg ttattcagca tgccggcgtt
     4981 gccgaacgaa cagtactcgc cgggcgggtg ctcggtgacg acggtgacgt cgaagccgtc
     5041 gcggcgcagg taggccgcgg tcgccatgcc gacgatgccg gcgccgatta ttgttacctt
     5101 cttcatgagc tattggtccc gcggagccgg gcgctggttc cgctcacggg cgtgaatggt
     5161 gaatggactg agcgcgcaaa gcgtgaacag ggtcaggtcg attcactatt aacgcagttg
     5221 agataatggt gggtggtaca gggatcgaac ctgtggcccc ttccgtgtga aggaagtgct
     5281 ctaccgctga gctaaccacc cgctgattaa ggcgtgagga tcattcggag cgcgcattct
     5341 atcaaaagaa cggctttccg gtcaccgatc acgccacttc gactcgcgcc gcacaatgtt
     5401 cccgctatgg ccgaaagggc gggcgttggg ttatggtagc aattcaatcg gaacccgtgg
     5461 cagcaatggc cgacgactac atcccgcttt accagccgcc cgaagcgcgc agcgagcctg
     5521 cgttgtggtt cgctttccgg aaatcggaaa tcctggtggt gagcggcgcg gaccggcccg
     5581 atctgccgtg ctgcatggaa ctcggcgagc acggcctgca gccgcagcgc agccagtatc
     5641 tcgggctcta cggcggccgg cattgctacg cggccgagat ccacgaatcg ctgccgctgc
     5701 cggcaggctg ggcggcattg ggcctgcgcg atcttttcgg gctggtggaa accacgctcg
     5761 cagcgctctc ggggcgcgcc tatcagatcc tcgagtggga ccgcaaccac cagtactgca
     5821 gccgttgcgg cacgcccacc cggctgcgca gcgacgagcg ctcgcgcacc tgccccgcct
     5881 gccagtacac gacctacccg ccggtcacgc ccgccatcat gatcctggtg acgcacggcc
     5941 gtaagcttct gctcgcgcgc aagccggcgt tccccaaggg gcgcttttcg gccctcgcgg
     6001 gtttcgtcga accgggcgaa atgctggagg atacggtgat ccgcgagacg cgtgaggaag
     6061 tcggcgtgga gatccgcaac atccgctatt tcggcagcca gccgtggccg tttccgcatt
     6121 cgctgatggt cgcctttacc gccgaatacg cgggcggcga ggtccggccg gatggcgtgg
     6181 agatcgagga ggcgcagtgg ttcgaagcgg gagagctgcc gaagctgccg ccgtcgatca
     6241 gcatctcgcg ccggttgatc gacaccgtcg ccggccggct cgcgcgcggt gaaccggcgt
     6301 caggagcgtg attcacgcct tttcgagctt gcgccacagc gtggcgccct tgctggcctt
     6361 gtcgatggcc tcgagttgcc gggcgtgggc ctcaagctcg gccggggtcg cggacacgat
     6421 gatcaggttc aatttgcccg tcgcgatctc ggcggctgcg gcgctctccg cggtcaccgg
     6481 cgcctccact tccatcatca ggctttcctg cccgcgggtc atggagagat agacctcggc
     6541 gagcagccgc gcgtcgagca gtgcgccgtg aagcgtgcgg gcggaattgt ctatctggta
     6601 gcgctcgcac aaggcgtcga ggccgtttct cttgccggga tgcagttccc gcgccatgcg
     6661 aagcgtgtcc accacgcccg agcagtactc ggtgacgggc ttcaagtcgg ccagatcgag
     6721 ttcccggttg aggaaggcga tatcgaacgc ggagttatga atgaccagtt ccgcgtcctt
     6781 gatgaaatcc aggaactccg cggcgacgtc ggtgaacttc ggcttgtcgg ccaggaactc
     6841 cgtcgtgagg ccatggatct cgagcgccgc ggcatcgctg tcgcgcccgg gattgaagta
     6901 gcggtggaaa tccttgcccg tgaagcgccg gttgacgagt tcgactcccg cgacctcgat
     6961 gacgcggtgg cccagctcgg gttccaggcc cgttgtttcg gtgtcgagga tgatctgcct
     7021 cattcctccg ccttcatcgc ttgttcatta cggattcaac cccctggttg gccagcgcgt
     7081 ccgcctgctc gttgccgggg ttgccgtcgt gtccccggac ccacagccat tcgatctcgt
     7141 gcttctccct cagcgcgtcg agttgccgcc ataaatcgtc gttctttacc ggtttcctgt
     7201 cggcggtgcg ccagccgcgg cgcttccacg aatggatcca ttccttgatc cccagctgaa
     7261 cgtactggga atcggtatgc acgcgggcgc gggtgccggg ctcgagcgtc tcga
//
LOCUS       DBNGSG010000313         4161 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4346341, whole genome shotgun sequence.
ACCESSION   DBNGSG010000313 DBNGSG010000000
VERSION     DBNGSG010000313.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4346341"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..1169
                     /locus_tag="AC158Q_08150"
     CDS             252..1169
                     /locus_tag="AC158Q_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3339788.1"
                     /translation="MTQRIEKVVAQVGGIILGKEGQIRLAIACLLARGHLLIEDIPGV
                     GKTTLAHALATSLGLAFQRIQFTSDLLPADIIGVSIYNRDTGGFQFHAGPIFSQVVLA
                     DEVNRATPKAQSALLEAMEEHQVTTEGETRRLPEPFFVIATQNPSYQVGTFPLPESQL
                     DRFLMRIHLGYPDGGAERALLQGQDRRDLLAVLTPAMSPGELAELQRTAQQIHVSGAL
                     LDYVQALADHTRRSADYVNGLSPRAALALLHAARAWAMMAGRGHVVPEDVQAVLPVVV
                     GHRLVPAGEVTRANGADIASRLIKQVAIP"
     gene            1283..2251
                     /locus_tag="AC158Q_08155"
     CDS             1283..2251
                     /locus_tag="AC158Q_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR3339789.1"
                     /translation="MYVALKNRLINWLFQLRGPEPGVIVLVQRRVFILPTRQGLLFAV
                     VLLVMLTGSINYALGLGFVLTFLLVALGVNAMIHTFRNLANLRITPGRSSPVFAGDIA
                     HFTVHLENDSDTERFAIGLTRDRKEATFVDVPARANRPASIGIPAARRGILRPGRLTL
                     FTRFPLGLYYAWSYLEFEMRCLVYPRPAFPGLPLPAATANAGAGAEHGRGQEDFAGLR
                     QYHVGDSPRHIAWKAAARDQGLLTKQFSGRADTELWLDWALLPAPLGVEERLSHLARW
                     VLDAHAAGISYGLRIPGRTVDMAVGEAQRDLCLEALALFESDKIEA"
     gene            2358..>4161
                     /locus_tag="AC158Q_08160"
     CDS             2358..>4161
                     /locus_tag="AC158Q_08160"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF023418.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase TgpA family protein"
                     /protein_id="HIR3339790.1"
                     /translation="MTDPKQALERRHVAYLLLSLTLVTVPHVFLVPWWTIGLVCALIA
                     WRAYLGYARLAMPNSWLLFLIAISAALGVFISYRTIFGRDAGVTLLVIMLGLKLLETR
                     TLRDAMLLIFLGYFLVITNFLRDQAIPTALYMLACTLVITAVMISLNYARAEPPFRAQ
                     LRRAGVMLAQSVPLMLVLFLLFPRVSGPLWGLPQDAFSGVSGLSDTMAPGSLSRLTLS
                     DAVAFRVKFASPMPRRRHLYWRGPVMWDFDGRTWSAARFFYNTPTIEATGEPVEYEIT
                     VEPHYKRWLFALDVPAMVPPLAFIGGDFQLYSSDPVNSRKRYDMRSYVNATYGMVENG
                     FSLRRALRLPSGPNPRTLEFARTLRAKHTDDLALVEEVLQMFRNQNYYYTLEPPLLGE
                     NSVDEFLFNTRTGFCEHYASAFAVLMRGAGIPARVVTGYHGGDLNEFGNYLIVRQAEA
                     HAWTEIWIRERGWIRIDPTSMVSPLRVDSGISAAVQGTDSLPFMIRTDFEVLRKLRLS
                     WDYMATAWNQWVLGYTPERQLQLMSRIGIDNATWQKLTAILLVLGGVIVAVFVALTLR
                     ELKGWVRDPVKAAYLRFCEKLRRKGLPRSPSEGPA"
BASE COUNT          701 a         1373 c         1291 g          796 t
ORIGIN      
        1 tgttgatgca ggccttgatg acggccgcgc gcgccgccgg cgtgcgcatc atgtacggcg
       61 acgtgctggc aagcaacaac aagatgctgc ggctcatggg caagatgggg tttaccgtga
      121 atttcaacga gcaggatgcg cgcatgatgc gcgtcgaaat caatctgtaa ggcggtggcg
      181 ccgtggttcc ggacacagga cgaaatccgc aggatatggc aaactcggcg cgtgaattga
      241 ttgcgccaaa catgactcaa cgaatcgaaa aagtcgtcgc ccaggttggc ggcatcatcc
      301 tcggcaagga aggccagatc cggctcgcga tcgcatgcct gctcgcgcgc ggccacctgc
      361 tgatcgagga catacccggc gtgggaaaaa ccacgctcgc gcacgcgctg gcgacttcgc
      421 tcgggctcgc gttccagcgc atccagttca ccagcgatct gctccccgcc gacatcatcg
      481 gggtctccat ctacaaccgg gataccggcg gttttcaatt ccatgccggg cccatcttca
      541 gccaggtcgt gcttgccgac gaagtcaacc gggcaacccc caaggcgcag agcgcgctgc
      601 tcgaagcaat ggaagagcac caggtgacca ccgagggcga aacgcggcgc ctgcccgagc
      661 catttttcgt gattgccacg caaaacccct cataccaagt cggcacgttt ccgctgccgg
      721 aatcccagct cgatcgcttt ctcatgcgca tacacctggg ctatccggac ggaggcgcgg
      781 aacgcgcttt gctgcagggc caggaccggc gcgacctgct cgcggtgctg acgcccgcca
      841 tgagcccggg ggaactggcg gaattgcagc gcaccgcgca gcagatacac gtgtcggggg
      901 ccttgctcga ttacgtgcag gcgctcgccg accatacccg ccgctcggcc gactacgtga
      961 acgggctgtc gccgcgcgcg gcgctggcgc tgctgcatgc ggcccgcgcc tgggcgatga
     1021 tggcgggacg cggccacgtc gtgcccgaag acgtgcaggc cgtgctgccg gtggtcgtcg
     1081 gccaccggct ggtacccgcc ggggaagtca cccgggccaa cggcgccgat atcgcgagcc
     1141 gacttatcaa gcaggtggcc ataccctgag agcgtgaatc gtgattggcg aagggtgatt
     1201 cgaaaaagca tgttgccgac gcgatagcga cgaggtattt acttttccgg cgactcacga
     1261 ctcacgaatc acgccattcg aaatgtacgt cgctctcaag aaccgcctta tcaactggct
     1321 gttccagctg cgcgggcccg agcccggcgt gatcgtgctg gtgcagcggc gggtgttcat
     1381 cctgcccacg cgccagggac tgttgtttgc cgtcgtgctg ctggtcatgc tgaccggatc
     1441 gatcaattac gcgctgggcc tcggcttcgt gctcacgttc ctgctggtag cgctgggcgt
     1501 gaacgccatg atccacactt tccgcaacct ggcgaacctc aggatcacgc ccggtcgctc
     1561 cagtcccgtg tttgccggcg atatcgcgca tttcaccgtt cacctggaaa acgacagcga
     1621 caccgagcgc tttgccatcg gactcacccg ggaccggaag gaagcaacct ttgtcgacgt
     1681 gcccgcgcgc gcgaaccgtc ccgcgtcgat cggaattccc gccgcgcgcc gcggcattct
     1741 tcgcccgggt cgcctgacgc tgttcacccg cttcccgctc ggtctctact acgcctggtc
     1801 ttatctcgag ttcgagatgc gttgtctcgt ctatccgcgc ccggcgttcc cgggactgcc
     1861 cctgccggcc gcgaccgcga acgccggtgc gggcgccgag cacggccgcg gccaggagga
     1921 ttttgccggc ctgcgccaat accatgtggg cgattcaccg cgccatatcg cgtggaaagc
     1981 ggccgcgcgc gaccaggggc tgcttaccaa gcagttcagc gggcgcgctg ataccgaatt
     2041 gtggctcgac tgggcgctgc tgcccgcgcc gctgggcgtg gaagaacgcc tgtcccacct
     2101 ggcgcgctgg gtgctcgacg cgcatgcagc aggaatttcc tacggcctgc gcatccccgg
     2161 caggacggtg gacatggcag tgggcgaagc ccagcgggac ctctgcctcg aagcgctcgc
     2221 gctatttgaa tcggacaaaa tcgaggcgtg aattgtgatt agtgaatgga ttcagccgtt
     2281 gacgatccgt ccggaaggac ggggggcgtg ttccattcac cgctcactat tcaccattca
     2341 cgattttacg tgtcgggatg actgacccca aacaggccct cgagcggcgc catgtggcgt
     2401 acctgctgct gtcgctcacg ctggtgaccg tgccgcacgt gttcctcgtg ccgtggtgga
     2461 cgatcgggct ggtgtgcgcc ctcatcgcgt ggcgcgccta tctgggatac gcgcgcctcg
     2521 ccatgccgaa cagctggcta ctgtttctca tcgcaataag cgccgcactc ggcgtcttta
     2581 tcagctaccg gaccatcttc gggcgcgatg cgggcgtaac gctgctcgtg atcatgctcg
     2641 ggctgaagct gctcgaaacg cgcacgctgc gtgacgcgat gctgctgatc ttccttggct
     2701 atttcctggt gatcaccaac ttcctgcgcg atcaggccat tcccacggcg ctctacatgc
     2761 tcgcgtgcac cctggtgatc accgcggtca tgatcagcct gaattacgct cgcgccgagc
     2821 cgccgtttcg cgcgcagctg cgcagggccg gcgtgatgct ggcccagtca gtgccgctga
     2881 tgctggtgct gttcctgctc tttccgcgcg tgtccggccc cctctgggga ctgccccagg
     2941 atgcattcag cggcgtgagc ggcttgtccg acaccatggc gccgggcagc ctctcccggc
     3001 tcaccctctc ggacgccgtt gcgttccggg tgaaattcgc atcgcccatg ccgcggcggc
     3061 gtcatctcta ctggcggggc ccggtgatgt gggatttcga tggccgcacc tggtcggcgg
     3121 cgcgcttctt ctacaacacc ccgaccatcg aggccacggg cgagccggtc gaatacgaaa
     3181 tcaccgtcga gccccactac aagcgctggc tctttgcgct cgatgtgccc gccatggttc
     3241 caccgctcgc gttcatcggc ggcgattttc agctctattc ctccgatccg gtcaacagcc
     3301 ggaaacgcta cgacatgcgc tcctacgtca acgccaccta tggcatggtc gaaaacggct
     3361 tttccctgcg ccgtgcgctg cgcctgccca gcggcccgaa tccgcggacg ctggaattcg
     3421 cgcgaacgtt gcgcgcaaag cataccgacg atcttgctct cgtcgaagaa gtccttcaga
     3481 tgttccgcaa ccagaactat tattacacgc tcgagcctcc cctgctcggc gagaactcgg
     3541 tggacgaatt cctgttcaac acccgcaccg gtttttgcga acactacgcc tccgccttcg
     3601 cggtgttgat gcgcggggcg ggcataccgg cgcgggtcgt gacgggatac catggcggcg
     3661 acctgaacga gttcgggaat tacctcatcg ttcgccaggc cgaagcccat gcctggacgg
     3721 aaatctggat ccgggaacgg ggctggatcc ggatcgaccc cacttccatg gtatcgccgc
     3781 tgcgcgtgga ctccggcatt tcggccgcgg tgcagggcac ggattccctg ccgttcatga
     3841 tccgcaccga cttcgaggtg ctgcgcaagt tgcggctctc atgggactac atggccaccg
     3901 cctggaacca gtgggtgctg ggttatacgc cggagcggca gcttcagctg atgtcccgca
     3961 tcggcatcga taatgcgacc tggcagaaac tcaccgccat tcttcttgtc ctgggaggcg
     4021 tgatcgtggc cgtgttcgtg gcactcacgc tgcgcgaact gaagggctgg gtccgcgacc
     4081 cggtcaaggc cgcttacctc cggttctgcg agaagctgcg gcgcaaggga ttgcctcgca
     4141 gcccgtcgga aggcccggcg g
//
LOCUS       DBNGSG010000314         8468 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1799818, whole genome shotgun sequence.
ACCESSION   DBNGSG010000314 DBNGSG010000000
VERSION     DBNGSG010000314.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 8468)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8468)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8468
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1799818"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1753
                     /locus_tag="AC158Q_08165"
     CDS             <1..1753
                     /locus_tag="AC158Q_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HIR3339791.1"
                     /translation="ARAILRMGVPVSPRNIFPSNIQGLPTWYEVRVNENGYVGRRGGV
                     DLMVAMNPQTWDNDLKEIEPGGYLFYDNSKPLPKSRFRDDINVLGMPLTEICNATYTD
                     PRQRQLFKNIIYVGALSALIDIDPAEIEKLFSEQYKGKEALLQSNVKALRLGRDYALQ
                     NLSCPLGIRIKRANNVGSRIFVDGNTAAALGAVYGGATVCAWYPITPSSSLAEAFIKH
                     CQKLRVDPATGKSRYAIIQGEDELASVGIAIGAGWNGARSFTATSGPGISLMTEFIGL
                     AYFAEIPVVLIDVQRGGPSTGMPTRTQQSDLLACAYASHGDTKHVLLFPEDPAECFDF
                     TARAFDLAERLQTPVFVMTDLDIGMNTRLCAPFQWDDTRTYDRGKVMSHDDLESGKDF
                     GRYLDVDGDGVPYRTYPGTHPTKGGYFTRGTTKDRYARYSETGPDYVYNMERLLRKFE
                     TAKKLVPHPLLTPAEKPARFGAIFYGSTAPAMYEALDVLSARGIHVNALRVRGFPFAD
                     EIADFAASHPWVFVVEQNRDAQLKTLLVNEAKVNPSRLLSVLHYDGTPITARYIVDQI
                     SQHAAARTVVPLKKVVS"
     gene            1750..2805
                     /locus_tag="AC158Q_08170"
     CDS             1750..2805
                     /locus_tag="AC158Q_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HIR3339792.1"
                     /translation="MTYLAKPKLHHPTLAKNQVGYTRRDYEGKISTLCAGCGHDSISA
                     AIIQACWELDIQPHRVAKLSGIGCSSKTPDYFLGNSHGFNTVHGRMPSVATGANLANR
                     DLIYMGVSGDGDSASIGIGQFAHCMRRGVNLVYIVENNGVYGLTKGQFSATADKGSKS
                     KRGVANTDEPLDMIGMALLLGATYVARSFPGDKKQLVPLIKGAIEHKGAAFIDVVSPC
                     VAFNNHPGSTKSYEYVRQHNEAVNRLDFIEGREEITADYAPGEAVTVTQHDGSMLRLR
                     KLATDYDPTDRIKAMNFLQERAARGEVITGLLYVQPDSADLHEHLNTVETPLNSLTER
                     ELCPGNVALERINASLR"
     gene            complement(2912..3244)
                     /locus_tag="AC158Q_08175"
     CDS             complement(2912..3244)
                     /locus_tag="AC158Q_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339793.1"
                     /translation="MKPINAIAVIFLFVAGIVAGIASASGGAPVRVFDAGELTPDRYT
                     VLERIWTGTWRTLFWIPGHDDAGAAVAALTDKARSLDADGVVNLHCLNDGGWGGGYYC
                     YALAIKLK"
     gene            complement(3416..4786)
                     /locus_tag="AC158Q_08180"
     CDS             complement(3416..4786)
                     /locus_tag="AC158Q_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIR3339794.1"
                     /translation="MQALKTGSDQGAQHFSLTKTHFRFLLISMMALLLAPLLFHAPLA
                     AAAEPPKLDSANTAWMLTSSVLVLFMTLPGLALFYAGLARAKNVLSILMQCFAIACMA
                     TLAWVVVGYSIAFGDAGGLNAWYGGFDKAFLSGIDVKTVKGSIPETVFSMFQLTFAII
                     TPALVIGAYAERVKFSGMLLFSLLWLLIVYCPVAHWVWGDGWLQKMGLMDFAGGTVVH
                     LNAGMAALVAALMLGQRRGFPETPMPPHNLAMTVTGASMLWVGWFGFNAGSALAADGA
                     AGMAMLVTHIGAATGAFAWMVCEWVKYGKPSVLGIVTGMVAGLGTITPASGFVGPLGA
                     LAIGGAAGVVCFVATNFMKRSLRIDDSLDVFPVHGVGGILGTILTGVFVASTFGGIGY
                     PEKVTMADQVLVQITGVLAVGAWSGIVTWLLLKLTDLVTGLRVTAEEESEGLDTVLHN
                     EKGYNL"
     gene            5059..6867
                     /gene="tfpB"
                     /locus_tag="AC158Q_08185"
     CDS             5059..6867
                     /gene="tfpB"
                     /locus_tag="AC158Q_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly ATPase TfpB"
                     /protein_id="HIR3339795.1"
                     /translation="MSTVKAAQPQTNRRLELAQVLADLAADGLVPKEVADDLAKNRRF
                     ARAEVHPLVVIADQKWKDPRGPKKILHLEALTQWLAEKVGLPYFHIDPFKIDFAAVTK
                     TMSSAYAERFRILPVAINTREVTIATSEPFVRDWEEQLKQVLRLEIKRVLANPIELQS
                     YLVEFYNLAQSVKGASAKDKSAISDIANFEQLVQLGRSGKLDANDQHVVHICDWLFNY
                     AFEERASDIHLEPRRDVGNVRFRIDGVLHQVYQIPTPVMGAMTSRIKVLGRMDVVEKR
                     RPQDGRLKTVTPDNQEVELRLSTMPTAFGEKLVMRIFNPENLVKDYKALGFPDEDERK
                     WTQMTSQPHGIILVTGPTGSGKTTTLYSTLKQLATPDVNVCTVEDPIEMIEPSFNQMQ
                     VQHSIGVDFASGIRTLMRQDPDIIMVGEIRDLETAEMAIQAALTGHLVLSTLHTNDSP
                     SAVTRMLDLGVPPYLLQSTILGVMAQRLLRTLCPHCKEQRELEDSAWEHLVSPWKAPK
                     PMGTTFTTKGCLECRMTGYMGRVGIYETMMMTNELRKAITAETDLAALRDKAYQEGMK
                     PLRLSGALKVAAGLTTIDEVLQTAPPPTTDRRQQPR"
     gene            6945..7370
                     /locus_tag="AC158Q_08190"
     CDS             6945..7370
                     /locus_tag="AC158Q_08190"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HIR3339796.1"
                     /translation="MKDTLKPGLAYQFKFKVPASKTVPALYPESESFQQMPQVLATGY
                     MVGLMEWACIEALRPHLDWPAEQSLGTQVNLSHLAATPPGLTVTVDVRLDRIEGRKLT
                     FSVSAHDGVDKITEGTHQRTVIDSARFTAKVAEKARLIS"
     gene            7383..7808
                     /locus_tag="AC158Q_08195"
     CDS             7383..7808
                     /locus_tag="AC158Q_08195"
                     /inference="COORDINATES: protein motif:HMM:NF015669.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HIR3339797.1"
                     /translation="MDLHLARLLAIAAVLAGCAHEPAARATLLETYWRPVEIEGHAVR
                     IPPGTREPHFVLSREGNRVRGFTGCNNLAGAFEQGADSFRFKQLVTTRMACLPDNDLE
                     ARFLSAVNATVAQRIVGAMLELRDQENRVRMRLEARHLK"
     gene            7829..8293
                     /locus_tag="AC158Q_08200"
     CDS             7829..8293
                     /locus_tag="AC158Q_08200"
                     /EC_number="5.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HIR3339798.1"
                     /translation="MAQAPYVPRAQESRNKTVVQRYFSEVLDQKKIELMPELMARDVV
                     LHRPGFDVTGLDAAMQRLRAVLQDYAAFSSELSGLIAEGDMVSVRVFHRVRARPHVFR
                     CRAGDVPIASERDLNWAAIVQFRLKDGKIAEEWVMRDELGMLAQMGKITVGG"
BASE COUNT         1681 a         2819 c         2566 g         1402 t
ORIGIN      
        1 cgcccgcgcc atcctgcgga tgggcgtgcc ggtcagcccg cgcaatatct tcccgtccaa
       61 catccagggc ctgcccacct ggtacgaggt ccgcgtcaac gagaacggtt atgtcggtcg
      121 ccgcggcggc gtggacctca tggtggcgat gaacccgcag acctgggaca acgacctgaa
      181 ggagatcgag cccggcggat atctcttcta cgacaattcc aagccgctgc cgaaatcgag
      241 gttccgggat gatatcaacg tcctcggcat gccgctcacc gagatctgca acgcgaccta
      301 caccgacccc cggcagcggc agctgttcaa gaacatcatc tacgtgggcg cgctctccgc
      361 tctgatcgac atcgatcccg cggagatcga gaagcttttt tccgagcagt acaagggcaa
      421 ggaagcgttg ctgcagtcca acgtgaaagc gctgcggctc ggccgggatt acgcactgca
      481 gaacctgagt tgcccgctcg gcatccgcat caagcgcgcc aataatgtcg gcagcaggat
      541 tttcgtggac ggcaataccg cggcggcgct gggcgcggta tacggcggcg ccacggtctg
      601 cgcctggtac ccgatcaccc cttcctcctc gctggcggaa gctttcatca agcactgcca
      661 gaagctgcgc gtggatcccg ccaccggcaa gagccgctac gcaatcattc aaggggaaga
      721 cgagctcgcc tccgtcggca tcgccatcgg cgcgggctgg aatggggcgc gctcctttac
      781 cgcgacctcg gggcccggca tctcgctgat gaccgagttc atcggtctcg cctacttcgc
      841 ggaaatcccg gtcgtgctga tcgacgtcca gcgcggcggg ccgtcgaccg gcatgccgac
      901 ccgcacccag caatccgacc tcctcgcctg cgcctacgcc tcgcacggcg acaccaagca
      961 cgtgctgctg tttccggaag acccggcgga gtgcttcgat ttcaccgcgc gggcgttcga
     1021 cctcgcggag cggctgcaga ctccggtatt cgtgatgacg gacctcgata tcggcatgaa
     1081 tacccgtctg tgcgcgccct tccagtggga cgacaccagg acctacgacc gcggcaaggt
     1141 catgagccac gatgacctgg agtccggcaa ggacttcggg cgctacctcg acgtggacgg
     1201 cgacggcgtg ccgtaccgca cttatcccgg cacgcacccc accaagggcg gctacttcac
     1261 gcgcggcacg acgaaagacc gttatgcgcg ctacagcgaa accgggccgg attacgtgta
     1321 caacatggag cggctgctgc ggaagttcga gaccgccaag aagctcgtgc cgcaccccct
     1381 gctcaccccg gccgaaaagc cggcccgctt cggggcgatc ttctacggtt cgaccgcgcc
     1441 ggcgatgtac gaggcgctcg acgtgctctc cgcgcgcggc atccacgtca acgcgctgcg
     1501 ggtgcgcggc ttccctttcg cggatgagat cgccgatttc gccgcatcgc atccgtgggt
     1561 attcgtggtc gagcaaaacc gcgacgccca gctgaaaacg ctgctcgtca acgaagccaa
     1621 ggtaaacccc tcgcggctcc tgtccgtgct gcactatgac ggcacgccga tcacggcgcg
     1681 ctacatcgtc gatcagatat cccagcacgc cgccgcccgc accgtcgtgc cgttgaagaa
     1741 ggtggtctca tgacttatct cgcgaaaccc aaactccacc acccgacgct ggccaagaac
     1801 caggtcggct acacgcgccg cgactacgag ggaaagatct cgacgctctg cgccggctgc
     1861 ggccacgact cgatctcggc cgccatcatc caggcgtgct gggaactcga tatccagcct
     1921 catcgcgtcg ccaagctctc cggcatcggc tgctcgtcga agacgccgga ttacttcctc
     1981 ggcaactcgc acggcttcaa caccgtccac ggccgcatgc cctcggtcgc caccggcgcc
     2041 aacctggcca accgcgactt gatctacatg ggcgtgtcgg gtgacggcga ttcggcctcg
     2101 atcggcatcg ggcagttcgc gcactgcatg cggcgcggcg tgaatctcgt gtacatcgtc
     2161 gagaacaacg gcgtctacgg cctcaccaag ggccagttct cggccaccgc ggacaagggc
     2221 tcgaagtcca agcgcggcgt cgccaacacc gacgagccgc tcgacatgat cggcatggcg
     2281 ctgctgctcg gcgccaccta cgtcgcgcgc agcttccccg gcgacaagaa gcagctggtg
     2341 cctctcatca agggggcgat cgagcacaag ggcgcggcgt tcatcgacgt ggtctcaccg
     2401 tgcgtcgcgt tcaataacca cccgggctcg accaaaagct acgaatacgt ccggcagcac
     2461 aacgaggcgg tcaaccggct cgacttcatc gagggccggg aggagatcac cgccgactac
     2521 gcgccgggcg aggccgtcac cgtcacccag cacgatggct cgatgctgcg cctgcgcaag
     2581 ctcgcgacgg actacgaccc gaccgaccgc atcaaggcga tgaatttcct gcaggaacgc
     2641 gccgccaggg gggaagtgat caccgggctg ctctacgtgc aacccgattc cgccgacctg
     2701 cacgagcacc tcaacaccgt cgagacaccg ctcaattctc ttaccgagcg ggaactgtgt
     2761 cccggcaacg tcgcgctgga gcgcatcaac gcctcgctgc ggtagaagcg tcaatcccgc
     2821 ggaagcggaa atccaggaag ccaggccacc atgctttgga tccccgcctt cgcggggatg
     2881 acggaccgcg ccgttcaggc cttgtggtcc gtcacttgag ctttatcgcc agggcgtaac
     2941 agtagtagcc gccgccccat ccgccgtcgt tcagacagtg caggttgacg acgccgtcgg
     3001 cgtccaggct gcgcgccttg tccgtgagcg cggccaccgc tgcccccgca tcatcgtgtc
     3061 ccggtatcca gaacagcgtg cgccacgtgc ccgtccagat tcgctcgagc acggtatagc
     3121 gatcgggcgt cagttccccc gcgtcgaata ccctgaccgg cgccccaccg gaagcgctgg
     3181 ctatccctgc aacaattccc gctacgaaca ggaatatcac tgcgatggcg ttgattggtt
     3241 tcatgggtga cgctccttag gcgcgcagat ctaacgtccg gactgacgaa aattatcgat
     3301 gacttggacg cgctcggcgc aacctgccca gcactgatac gcgcgatgag cggcgcagcg
     3361 catccgctcg atcgcgcgta agcaaaccgg cgcgccgcca gcgcgtccgg atttgctaaa
     3421 ggttgtagcc cttctcgtta tgcagcaccg tgtcgaggcc ttcggactcc tcttccgccg
     3481 tcacgcgcag tcccgtcacg aggtcggtga gcttgaggag cagccaggtc acgattccgg
     3541 accacgcccc caccgccagc acgccggtaa tctgcaccag cacctggtcc gccatcgtta
     3601 ccttctcggg atagccgatg ccgccgaacg tggaggccac aaacacgccg gtgagtatcg
     3661 tgccgaggat cccgccaacg ccgtgaaccg ggaatacgtc gagcgagtcg tcgatacgca
     3721 gggaccgctt cataaaattg gtggcgacga agcacaccac gccggcggca ccgccaatgg
     3781 ccagcgcgcc gagcggtccg acgaagcccg atgccggtgt gatcgtgccc aggccggcga
     3841 ccatcccggt cacgatgccg agcaccgagg gcttgccgta tttcacccat tcgcagacca
     3901 tccaggcgaa agccccggtc gccgcgccta tgtgcgtcac cagcatcgcc atgcccgccg
     3961 cgccgtcggc cgcaagtgcg ctgcccgcgt tgaaaccgaa ccagcccacc cacaacatgc
     4021 tcgcgccggt cacggtcatg gcgaggttgt gcggcggcat gggcgtctcg ggaaatcccc
     4081 tgcgctgtcc cagcatcagc gcggccacca gcgccgccat ccccgcattc aggtgcacga
     4141 ccgtgccgcc cgcgaaatcc atcaagccca ttttctgcaa ccagccgtct ccccagaccc
     4201 agtgcgcaac cgggcaatag acgatcagca gccagagcag gctgaacagg agcatgccgg
     4261 agaacttcac gcgttcggcg taagcgccga tgaccagcgc cggagtgatg atggcgaacg
     4321 tcagctggaa catggagaac acggtctccg ggatgctgcc cttgaccgtc ttgacgtcga
     4381 tgccggacag aaaggccttg tcgaatcccc cgtaccaggc attgagcccg cccgcgtctc
     4441 cgaacgcaat gctatagccg accacaaccc acgccagtgt agccatgcag gcaatagcga
     4501 agcactgcat gagtattgaa agcacgttct tcgcccgggc cagcccggcg tagaacaagg
     4561 ccaggccggg gagcgtcatg aacaggacca gcaccgagga cgtgagcatc cacgccgtgt
     4621 tcgcgctgtc cagcttcggc ggctcggccg ccgcggcgag cggcgcatga aacagcaacg
     4681 gggccagcag cagggccatc atggaaatca gaagaaaacg gaagtgggtc ttcgtgagcg
     4741 aaaaatgctg cgccccctgg tcgctgccgg ttttcagtgc ttgcatggcg tgtccccctt
     4801 ttggtttaac ccggaataat aagcaaaagg cattcctcat gactatgtca ttgatagtca
     4861 tcgattatga attttcagcg tcacaaaatg gcaccggtta agtgcattat ggtattttgt
     4921 tgcacgttaa cggtgcaaac gggaccctgc atcaccgcgg cagcgcggaa ctagactgcg
     4981 tggcaggtgt tttccgtaac ccattgatag atttaggtct gcgggttata gtcgcgctca
     5041 actcagggaa ttgacgcgat gtccaccgta aaagccgccc agccccagac caaccgccgc
     5101 ctggagctgg cgcaggtgct cgcggacctg gcagccgacg gcctggtgcc caaggaagtt
     5161 gccgacgatc tcgccaagaa ccggcggttc gcccgtgccg aggtgcatcc gctggtggtc
     5221 atcgccgacc agaagtggaa ggacccgcgc ggcccgaaga agatcctgca cctcgaagcg
     5281 ctcacgcagt ggctggcgga gaaagtcggc ctgccctatt tccacatcga tcctttcaag
     5341 atcgatttcg ctgcggtgac caagacgatg tcgagcgcct acgccgagcg cttccgcatc
     5401 ctgccggtcg ccatcaacac gcgcgaggtc accatcgcca ccagcgagcc gttcgtgcgc
     5461 gactgggaag agcagctgaa gcaggtgctg cgcctggaga tcaagcgcgt cctcgccaac
     5521 cccatcgagc tgcaaagcta cctggtcgag ttctacaacc tggcgcagtc ggtcaagggc
     5581 gcaagcgcga aggacaagag cgcgatcagc gacatcgcca acttcgagca gctggtgcag
     5641 cttggaagga gcggcaagct cgacgcgaac gaccagcacg tcgtgcacat ctgcgactgg
     5701 ctgttcaatt acgctttcga ggagcgcgcg agcgatatcc acctcgaacc gcggcgcgac
     5761 gtcggaaacg tgcgatttcg catcgacggc gtgctgcacc aggtctacca gatccccacg
     5821 ccggtgatgg gggcgatgac cagccgcatc aaggtactgg ggcgcatgga cgtggtcgag
     5881 aaacggcgcc cccaggacgg gcgcctgaag accgtcaccc ccgataacca ggaggtggag
     5941 ctgcggctgt ccaccatgcc caccgcgttc ggcgagaagc tcgtgatgcg gatcttcaac
     6001 cccgagaatc tggtgaagga ctacaaggcg ctcgggttcc cggacgaaga cgagcgcaaa
     6061 tggacgcaga tgacgagcca gccgcacggc atcatcctgg tgaccggccc caccggttcg
     6121 ggcaagacca ccaccctcta ctcgacgttg aagcagctcg ccacgccgga tgtgaacgtc
     6181 tgcaccgtgg aggatccgat cgaaatgatc gagccctcct tcaatcagat gcaggtgcag
     6241 cactcgatcg gcgtggactt cgcgagcggc atccgcacgc tgatgcgcca ggatccggat
     6301 atcatcatgg tgggcgagat ccgcgatctc gagaccgccg aaatggcgat ccaggcggcg
     6361 ttgaccgggc acctcgtgct ctcgaccctg cacaccaacg attcgccctc ggccgtcacc
     6421 cggatgctgg atctcggcgt gccgccttac ctgctgcagt ccaccatcct cggcgtaatg
     6481 gcgcagcggc tgctgcgcac gctctgccct cactgcaagg aacagcgcga actggaagat
     6541 tccgcgtggg agcacctggt atcgccctgg aaagcgccca agcccatggg caccaccttc
     6601 actaccaagg gctgcctcga gtgccgcatg acgggctata tggggcgcgt cggcatctac
     6661 gagacgatga tgatgacgaa cgagctgaga aaggcgatta cggccgaaac cgacctggcc
     6721 gcgctgcggg acaaggccta ccaggaaggc atgaagccgc tgcggctcag cggcgccctg
     6781 aaagtcgccg ccgggctcac caccatcgac gaggtgctgc agaccgcccc gccgcccacc
     6841 accgaccggc gccagcaacc cagataagca agcgtgacgg cggaagggtg acgagtgatg
     6901 ggtgataata acgacgctcc tcgattcccg ccatgcatca gccgatgaaa gacacgctca
     6961 agcccggcct cgcttaccag ttcaagttca aggttcccgc cagcaagacc gtccccgcgc
     7021 tctatcctga atcagaatcc ttccagcaga tgccgcaggt gctggccacg ggctacatgg
     7081 tcgggctgat ggaatgggcg tgcatcgagg cgctgcggcc ccacctcgac tggcccgccg
     7141 agcagtcgct cggcacccag gtcaatttat cccacctggc ggcgacaccg cccggactca
     7201 ccgtgaccgt ggacgtcagg ttggacagga tcgagggccg caagctcact ttctctgttt
     7261 cggcgcacga cggcgtggac aaaatcaccg agggcacgca ccagcgcacg gtgatcgaca
     7321 gcgcgcgctt cacggcgaag gtggctgaaa aggcgcggct gatcagctga cggcaatcgg
     7381 tcatggacct ccatctggca cgtcttctgg cgattgccgc agttctggcc ggctgcgcgc
     7441 acgagccagc tgcgcgggcc acgctgctgg agacttactg gcggccggtc gagatcgaag
     7501 gccacgcggt aaggatcccg cccgggaccc gcgagcccca tttcgtgctg tcgcgcgagg
     7561 gaaatcgcgt gcgcggattt accggctgca acaacctcgc cggcgctttc gagcagggcg
     7621 ccgacagctt ccggttcaag caactcgtca cgacgcggat ggcctgcctg ccggacaacg
     7681 acctcgaagc gcgctttctg tcggcggtca acgccaccgt ggcgcagcgt atcgtcggag
     7741 caatgctcga actgcgcgac caggaaaaca gggtccgcat gcggctcgag gcgcgtcacc
     7801 tgaaatagct gcgggaggaa ccagagttat ggctcaagcg ccttacgtgc cgcgagctca
     7861 ggagagccgc aacaagaccg tcgtgcagcg ctacttcagc gaggtgctcg accagaagaa
     7921 aatcgagctc atgcccgaac tcatggcgcg cgacgtcgtc ctgcaccggc cgggcttcga
     7981 cgtgaccggc cttgacgcgg cgatgcagcg cctgcgcgcg gtgctgcagg actacgcggc
     8041 cttttcttcc gagctgtcgg gcctgatcgc ggaaggcgac atggtgtcgg tgcgcgtctt
     8101 tcatcgcgtt cgcgcgcgcc cgcacgtctt ccgctgccgg gcaggcgatg taccaatcgc
     8161 ttccgaacgc gacctaaact gggcagcgat cgttcagttc cggctgaagg acggcaagat
     8221 agccgaggaa tgggtgatgc gcgacgagct cggcatgctc gcgcagatgg ggaaaattac
     8281 ggttggcggc tgacgcccgc tgccgcgtcg atttaaaccc gccaacaccg taaatggaca
     8341 ggattaacag gattcaggaa ggcaaatcat tcgtctgtag gtcttaatct tgtaaatcct
     8401 gttaatcctg tctattgatg ggtttgacgg gttttcgagc ggggtcgatc ttgtaaatcc
     8461 tgtctcaa
//
LOCUS       DBNGSG010000315         7292 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2310480, whole genome shotgun sequence.
ACCESSION   DBNGSG010000315 DBNGSG010000000
VERSION     DBNGSG010000315.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7292)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7292)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7292
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2310480"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1324)
                     /locus_tag="AC158Q_08205"
     CDS             complement(<1..1324)
                     /locus_tag="AC158Q_08205"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3339799.1"
                     /translation="MNADQIEIDRPALERAERESGYMALKFKSILFVAETLHRRFFIG
                     VHRRSSAVAFFLMLAALPAWAILPIQHWQTANGARVYFVENRDLPMLDVSVEFPAGAG
                     YDAADKAGIAAMVNRLLTAGADGLTEEDIAQRLADVGAQSGGRFDTDRAGFALRTLTS
                     PRERRQSLDIMARLLSGPVFPQTVLEREKVRLIGALKEADTRPDTIASRNFYRMVYRD
                     HPYALRASGEPDTVEKITREELSAFYRRHYAAPYAVVAMIGDVTRAEAETIAEQLTRG
                     LPAASGAEPVLPPVTDLSAGQTRIIAHHATQAHILIGAPGIRRDDPDYFPLFVGNHVL
                     GGGGFVSRITNEVRQKRGLAYSAYSYFSPLKSAGPFVIGMQTQRDQAGEALKVADTVL
                     REFLERGPTDTEVAAAKKGIVGGFPLRIDTNRKIHGYLALIGFYRLPLT"
     gene            complement(1341..2720)
                     /locus_tag="AC158Q_08210"
     CDS             complement(1341..2720)
                     /locus_tag="AC158Q_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3339800.1"
                     /translation="MTFIRKLIGVLGALALAAPPGLAADDTTERKLANGLRVIVKPDR
                     RAPVAVVMAWYRVGSIDEVNGVTGVAHVLEHMMFKGTSATPAGEYSRIISAAGGRDNA
                     FTSNDYTGYFAMLQKTHLELALKLEADRMTNLTLAPGEFAKEIKVVMEERRLRTEDRP
                     RSLVYEQLMATALTAHPYRNPIIGWMNDLENMRVEDARAFYRQWYAPNNVVLVVVGDV
                     EPGQVFGLVEKHFGGIPSRPLPPRKPQEEPAQLGAKRITVKAPAELPYVMMAFRVPVL
                     RDPERDWEPYALDMLAAVLSGNETARLNRRLVREQRVASSAGASYDGIARGPDFFFLS
                     ATPTPGKSVAEVEQGLRREMARIIGEGVTVEELDRVKAQAIAAEVYQRDSMFFQARVI
                     GTLEMTGLSHRTVDLQLAKLREVTPEQVREVARKYFREDALTIAYLDPQPLSGKRPAP
                     PPAGLRHTQ"
     gene            2840..3820
                     /gene="ftsY"
                     /locus_tag="AC158Q_08215"
     CDS             2840..3820
                     /gene="ftsY"
                     /locus_tag="AC158Q_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIR3339801.1"
                     /translation="MLSFLKSSDGGGWFTRLRDGLAKTREQLGGRFSGLFGTTRPLDE
                     AFYEELETALIAADVGVAASAHLLAELRRRARREGITDSTRLKQALQQTLLDLLQPVA
                     VPLEVEAHRPFVIMLAGVNGSGKTTSIGKLAHYYQSRGKSVLLAAGDTFRAAAREQLV
                     AWGERNNVAVITQRSGDAAAVIYDAVNAAIARGIDVVLADTAGRLPTQLHLMEEIRKV
                     KRVIDKALPGAPHEVLLVLDANTGQNALAQVRAFDATIGVTGLVLTKLDGTAKGGVVA
                     SIAHARSSVALARSSASGEKPIPVRFIGVGEGLDDLRPFNAGEFVAALFD"
     gene            3907..4560
                     /gene="ftsE"
                     /locus_tag="AC158Q_08220"
     CDS             3907..4560
                     /gene="ftsE"
                     /locus_tag="AC158Q_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HIR3339802.1"
                     /translation="MISFSQVTKRYPSGLEALKGVTLTIEAGELVFITGHSGAGKSTL
                     LKLAAAIERPSSGSVIVNGQNVGALRARAVPFLRRNFGLIFQDQKLLFDRSAFDNVML
                     PLAITGFERREAARRVRAALDKVGLLPKEKFFPVTLSGGEQQRLAIARAIVHRPAILV
                     ADEPTGNLDTAYAAELGELFRSFNQVGVTVVIATHDEAFAKRLKPRLVALKEGRLAK"
     gene            4557..5462
                     /gene="ftsX"
                     /locus_tag="AC158Q_08225"
     CDS             4557..5462
                     /gene="ftsX"
                     /locus_tag="AC158Q_08225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HIR3339803.1"
                     /translation="MTWFWHHLDALRVTLARLARTPVVTLLNVGVIGIALALPVGLYV
                     ALVNLQGFAHTLASDPQLSVFLALDAGRADTEKIAARLKNHAGVRAARYVSREQALKE
                     LKASTGLADVVDSLPQNPLPDAFVVLPRDTQPQALEKLRDEVRGWPKVTHVQLDTAWA
                     RRLEAGLKLARLAVGLLATMFAFALVAVTFNTIRLQILTRREEIEVSKLIGATDPFIR
                     RPFLYFGALQGLAGGIAAWAIVWVGVWLLNGALAELAQLYASRFELRHLGLEDSLSLL
                     AFSAALGWFGSWLSVNQHLAAIEPR"
     gene            5605..6459
                     /gene="rpoH"
                     /locus_tag="AC158Q_08230"
     CDS             5605..6459
                     /gene="rpoH"
                     /locus_tag="AC158Q_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HIR3339804.1"
                     /translation="MSSLVLPVPSAVGSLESYVQSVNRFPLLTQEQELDFARRYRKEG
                     DLDAARELVVSHLRVVVAISRGYLGYGLPQADLIQEGNIGLMKAVKRFDPERGVRLVS
                     FAVHWIRAEMHEYILRNWRIVKVATTKAQRKLFFNLRSLKQSFNSLGFEDVRKVAKQL
                     GVKPEEVVEMETRLTGRDVAIEPAGDDEEDKFSPISYLAAEDAEPGRILEHEETARLR
                     SQGLGEALAGLDARSRRIVEARWLAEKDPATLHDLAAEFGVSAERIRQIETKALARMK
                     GVIARQQA"
     gene            complement(6527..7108)
                     /locus_tag="AC158Q_08235"
     CDS             complement(6527..7108)
                     /locus_tag="AC158Q_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR3339805.1"
                     /translation="MRFLPALALSALLAACGGGAEPPRFKLTDVSGVAFGKELNLTDH
                     NGKPRTLADFRGKVVMVFFGFTHCPDVCPTTLAEMAQVAKALGPDADKLQVLFVTVDP
                     ERDTPQVLKQYVPSFNPAFLGLSGSLDATARAAKEFKVFYQKQPARNGGYSVDHGAGT
                     FILDPQGRLRLFAQYGQGAPVLLHDIRLLLAGA"
BASE COUNT         1233 a         2469 c         2374 g         1216 t
ORIGIN      
        1 aggtgagcgg caggcggtag aagccgatca aagcgaggta gccgtggatc ttgcggttgg
       61 tgtcgatgcg cagcgggaac ccgccgacga tgcccttctt ggctgccgcc acctcggtgt
      121 cggtcgggcc gcgctcgagg aattcccgca gcaccgtgtc agcgaccttg agcgcctcgc
      181 cggcctggtc gcgctgcgtc tgcatgccga tcacgaacgg gcccgcgctc ttcaatggcg
      241 agaagtagct gtaggccgaa taggccaagc cgcgtttctg ccgaacttca ttggtgatgc
      301 gcgacacgaa gccgccgccg cccagcacgt ggttgccgac gaacaacggg aaataatccg
      361 ggtcgtcgcg gcggatgccg ggagcgccga tcaggatgtg cgcctgcgtg gcgtggtgag
      421 cgattatgcg cgtctgcccg gcggacagat cggtgaccgg gggcagcacc ggctccgcgc
      481 ctgaagccgc cggcagccct cgcgtcagct gctcggcaat cgtctcggct tccgcgcggg
      541 tcacgtcccc gatcatggcc accaccgcat aaggcgcggc gtagtggcgc cggtaaaatg
      601 cggacagctc ctcgcgcgtg attttctcga ccgtatcggg ctcgcccgag gcgcgcagcg
      661 cgtacggatg gtcgcggtag accatgcgat aaaagttgcg cgacgcgatg gtatcggggc
      721 gggtgtcggc ttccttcagc gccccgatca gccggacctt ttcgcgctcc agcaccgttt
      781 gcgggaaaac cggcccgctc aggagcctgg ccatgatatc gagcgactgg cggcgttccc
      841 gcgggctcgt cagcgtgcgc agggcaaacc cggcccggtc ggtatcgaag cgccccccgg
      901 actgggcgcc gacatcggcc aggcgctgcg cgatgtcctc ctcggtaaga ccgtccgcgc
      961 ccgcagtgag caggcgattc accatggccg cgatgccggc cttgtccgcg gcgtcgtatc
     1021 ccgcccctgc cggaaactcc acgctcacgt cgagcatcgg caggtcgcga ttctcgacga
     1081 aatagacgcg cgccccgttc gcggtctgcc agtgctgtat cggcaggatc gcccaggcgg
     1141 gcagcgccgc caacatcaag aagaatgcaa ccgccgatga acgccgatga acgccgatga
     1201 aaaacctccg atgcaaagtc tcggcgacga aaagaatcga cttgaacttc aaggccatgt
     1261 acccggattc ccgttccgcg cgctccaggg caggcctgtc gatttcgatc tgatcggcgt
     1321 tcatcggcgg ttcccgggtt ttactgcgta tggcgcaggc ccgcgggcgg cggcgccggg
     1381 cgtttgcccg aaagcggctg ggggtcgagg taagcgatgg tgagcgcatc ctcccggaaa
     1441 tattttcgcg cgacttcgcg cacctgctcc ggcgtgactt cccgcaactt cgcgagctga
     1501 agatccaccg tccggtgcga caggccggtc atctccagcg tgccgatcac gcgcgcctgg
     1561 aagaacatcg aatcgcgctg gtaaacctcg gcggcgatcg cctgcgcctt cacccggtcg
     1621 agttcctcga cggtcacccc ttcgccgatg atccgcgcca tttcgcgcct caggccctgc
     1681 tccacctcgg ccacgctctt tcccggcgtc ggcgtcgcgc tgagaaagaa gaaatccggc
     1741 ccgcgcgcga tgccgtcgta gctcgcgccc gccgaactcg ccacgcgctg ctcgcgcacc
     1801 aggcggcggt tcagacgtgc ggtctcgttg ccgctcagga cagccgccag catatccagc
     1861 gcatacggct cccaatcgcg ctcggggtcg cgcagcaccg gcacccggaa cgccatcatc
     1921 acgtacggca gctccgcggg cgccttgacg gtgatgcgct tcgcgcccag ctgcgccggc
     1981 tcctcctgcg gcttgcgcgg aggcagcggg cgcgacggga tgcccccgaa atgtttctcg
     2041 acgagaccga acacctgccc gggctcgacg tcgcccacca ctaccagcac cacgttattg
     2101 ggcgcatacc actgccggta gaacgcgcgc gcgtcctcga cccgcatgtt ctccaggtcg
     2161 ttcatccagc cgataatggg attgcggtac ggatgagcgg tcagcgccgt tgccatcagc
     2221 tgctcgtata ccagcgaacg cggccggtcc tcggtgcgca gccggcgctc ctccatcacc
     2281 accttgattt ctttcgcgaa ctccccgggc gcgagcgtaa ggttcgtcat gcggtcggct
     2341 tcgagcttca acgcgagctc caggtgggtt ttctgcagca tggcgaagta gccggtgtaa
     2401 tcgttgctgg taaacgcgtt atcgcgcccg cccgcggcgg agatgatccg ggaatattcc
     2461 cccgccgggg tcgcggatgt gcccttgaac atcatgtgct cgagcacgtg ggcgacgccg
     2521 gtgacgccgt tcacttcgtc aatgctgccg acgcggtacc acgccatcac caccgccacc
     2581 ggcgcgcgcc ggtcgggctt cacgatgacg cgcaggccgt tggcaagctt gcgctcggtg
     2641 gtatcgtccg ccgccaggcc tggcggggcc gcgagcgcca gcgcgcccag cacgccaatc
     2701 agcttcctga tgaatgtcac gggattgttc ttcgacgagg tgggtttcag gctagaattg
     2761 tatccactcg cgggccggat aggacaactt tcttggcgcg gcccgcggat tttccatctg
     2821 cctctagact gcgcaaccct tgttaagttt cctcaaatcg tccgatggcg gcggctggtt
     2881 cacgcgcctg cgggacggtc tcgccaagac acgcgagcaa ctgggcggcc gcttctcggg
     2941 gctgttcggc acgacgcgcc cgctggacga ggcgttctac gaggaactgg aaacggcgct
     3001 gatcgccgcc gacgtcgggg tggcggcgag cgcgcacctg ctcgccgagt tgcggcgccg
     3061 cgcccggcgc gagggcatca ccgattccac gcgcttgaag caggcgctgc agcaaaccct
     3121 gctcgacctg ctccagccgg ttgcggtccc gctcgaggtg gaggcccacc gcccgttcgt
     3181 gatcatgctg gccggcgtca acggcagcgg caagaccact tcgatcggca agcttgccca
     3241 ttattaccag agccgcggca agtccgtgct gctcgcggcg ggcgacacct tccgcgctgc
     3301 cgcgcgcgag cagctcgtcg cgtggggcga gcgcaacaac gtcgccgtaa tcacgcagcg
     3361 gtccggggac gcggcggcgg tgatctacga cgcggtcaat gcggcaatcg cccgcggcat
     3421 cgacgtggtg ctggccgaca ccgcgggacg gctgccgacc cagctccacc tcatggagga
     3481 aatccggaag gtcaaacgcg tgatcgacaa agcgttgccg ggtgcgccgc acgaagtgct
     3541 gctggtgctc gacgccaata ccggccagaa cgcccttgcc caggtccgcg cgttcgacgc
     3601 gacgatcggg gtgaccggac tcgtcctgac caagctcgac ggcaccgcca agggcggcgt
     3661 ggtcgcttcc atcgcgcatg cgagatcctc cgtcgcgctt gcgcgatcca gtgcctccgg
     3721 ggagaagccc atacccgtgc gtttcatcgg cgtcggcgag ggtctcgacg acctgcggcc
     3781 gttcaacgcc ggcgaattcg tcgccgccct cttcgattga cccggggccg ctcgggcacc
     3841 aacgaaagcc gcgttacagc caccccagtg agtgcccact gacctcctcc ggctccggat
     3901 aactgcatga tttcattcag ccaggtcacc aagcgctacc cgagcgggct ggaagccctg
     3961 aagggcgtca cactgacgat cgaagccggt gaactggtct tcatcaccgg ccattccgga
     4021 gccggcaaga gcacgctgct caagctcgcg gcggcgatcg agcggccgtc gagcggcagc
     4081 gtcatcgtca acggccagaa cgtgggggcg ctgcgggcgc gcgccgtgcc attcctgcgc
     4141 cgcaatttcg gcctcatctt ccaggaccag aaactgctgt tcgaccgcag cgcgttcgac
     4201 aacgtaatgc tgccgctcgc catcaccggc ttcgagcggc gcgaggccgc ccggcgcgtg
     4261 cgcgcggcgc tcgacaaggt cggcctgctc cccaaggaaa aattcttccc ggtcacgctc
     4321 tccggcggcg agcagcaacg gctcgcgatc gcgcgcgcca tcgtgcaccg tcccgccatc
     4381 ctggttgccg acgagcccac cggcaatctc gacaccgcct acgccgccga gctgggcgag
     4441 ctgttccggt cgttcaacca ggtcggcgtc accgtggtca tcgcgaccca cgacgaagcc
     4501 ttcgccaagc gactgaagcc caggctcgtc gcgctcaagg aaggaaggct ggcgaaatga
     4561 cctggttctg gcaccacctt gacgccctgc gcgtgacgct cgcgcgtctc gcgcgcacgc
     4621 cggtcgtgac gctgctcaac gtgggcgtga tcggcatcgc gctcgcgctg ccggtcgggc
     4681 tttacgtcgc tctcgtcaac ctgcagggct tcgcgcacac gctcgcctcc gatccccagc
     4741 tctcggtgtt cctcgccctc gatgccgggc gcgccgacac cgagaaaatc gcggcgcgcc
     4801 tgaagaacca cgcaggcgtg cgcgcggcgc gttacgtgtc gcgcgagcag gcgctgaaag
     4861 agctcaaggc gagcaccggg ctcgccgacg tcgtggacag cctgccgcag aacccgctgc
     4921 cggacgcctt cgtggtgctg ccgcgggaca cgcaaccgca ggcgctcgag aaactgcgcg
     4981 acgaggtgcg cggatggccc aaagtcacgc acgtgcagct cgacacggcg tgggcgcggc
     5041 gcctggaggc gggactgaag ctcgcgcggc tggccgtggg cctgctggcg acgatgttcg
     5101 ccttcgcgct ggtggcggtg acattcaata ccatccgcct gcaaatcctc acccgccgcg
     5161 aagagatcga ggtctccaag ctgatcggcg cgaccgaccc ctttatccgc cgcccatttc
     5221 tttatttcgg cgcgctgcag gggctggccg gcggcatcgc agcctgggcg atcgtctggg
     5281 tgggcgtgtg gctactgaat ggcgcgctcg ccgaactggc gcagctctat gcctcccgct
     5341 tcgagctacg gcacctcggt ctggaggaca gcctgtccct gctcgcgttc tccgccgcgc
     5401 tgggctggtt cggctcctgg ctctcggtca accagcacct ggcagcgatc gagccccgct
     5461 agggcggcag cgtgtccaac cccctgatta tgggcctgtt ttagcctcaa aatgccgact
     5521 taacacactg aatacaagga actttcatgg attctggcac tcttaatcct tgagtgctaa
     5581 aattgcaggc acagaggggg ttccatgagc agcctggtgt taccggtccc atcggcagtc
     5641 ggcagcctgg agagctacgt ccagagcgtc aaccgcttcc cgctcctcac ccaggagcag
     5701 gagctggatt tcgcccgccg ctatcgcaaa gagggtgacc tcgacgccgc gcgggaactg
     5761 gtggtctcgc acctgcgggt ggtggtcgcg atctcccgcg gctacctggg ttatggcctg
     5821 ccgcaggccg acctgatcca ggaaggcaat atcggcctca tgaaggcggt caagcgcttc
     5881 gaccccgagc gcggcgtcag gctggtctcg ttcgccgtgc actggatccg cgccgagatg
     5941 catgaataca tcctgcgcaa ctggcgcatc gtgaaggtcg cgaccaccaa ggcgcagcgc
     6001 aagctcttct tcaatctgag gagcctcaag cagagcttca acagcctcgg cttcgaggac
     6061 gtcaggaaag tggcgaagca gcttggcgtc aagcccgaag aggtcgtcga gatggaaacg
     6121 cggttgaccg gcagggatgt cgcgatcgag cccgcgggcg acgatgaaga agacaagttc
     6181 tcgccgattt cgtacctcgc ggcggaagac gccgagcccg gccgcatcct cgagcacgag
     6241 gaaaccgcgc ggctgcgcag ccaagggctg ggagaggcgc tcgcaggcct ggacgcgcgc
     6301 agccgccgca tcgtcgaggc gcgctggctc gccgagaagg atcccgcgac gctgcacgat
     6361 cttgccgccg aattcggcgt ctccgccgag cgcattcgcc agatcgaaac gaaggcactc
     6421 gccaggatga aaggcgtcat cgcccgccag caggcgtaac tgattcggga aagcgtgtcc
     6481 gacaaagccc cggcgccgcc ggggcttttt tacgtccagg gtatttctac gcgccggcga
     6541 gcagtagccg gatatcgtgc agcagcaccg gggcgccctg cccgtactgg gcgaacaggc
     6601 gcagccttcc ctgcgggtcg aggatgaagg tgcccgcgcc gtgatccacg ctgtagccgc
     6661 cattcctggc cggctgtttc tggtagaaca ccttgaattc cttcgccgcg cgggcggtgg
     6721 catcgaggct cccggagagc cccaaaaaag cgggattgaa cgacggcacg tactgtttca
     6781 gcacctgcgg cgtgtcgcgc tccgggtcca ccgtgacgaa cagcacctgc agcttgtcgg
     6841 cgtcggggcc gagtgccttg gccacctgcg ccatctcggc gagcgtcgtc gggcagacgt
     6901 cggggcagtg cgtgaatccg aaaaacacca tcaccacctt gccgcggaaa tccgccagcg
     6961 tgcgcggctt tccattgtga tcggtgaggt tgagctcttt gccgaaagcc actcccgaga
     7021 cgtcggtgag tttgaaccgg ggtggttccg cgccgccgcc gcaggccgcg agcagcgccg
     7081 acaatgccaa tgcagggaga aacctcatgg gaaaggcgcg gacctcgcgc cgctacgacg
     7141 gcacgcggat atagtgatcg gcgagcagcg cggcgaacat cgccgtgaga taaaagatgg
     7201 aatagcggaa cgtgcgctgt gcgagccggt cggtgtaggc gacgtagatg cgcaccgcgt
     7261 accacaggaa cacggcgttc agcacgatcg cg
//
LOCUS       DBNGSG010000316         1337 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_612576, whole genome shotgun sequence.
ACCESSION   DBNGSG010000316 DBNGSG010000000
VERSION     DBNGSG010000316.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1337)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_612576"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..719)
                     /locus_tag="AC158Q_08240"
     CDS             complement(33..719)
                     /locus_tag="AC158Q_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339806.1"
                     /translation="MRELEGIWIKGQYLEALEKSRMPHQTARKVPPVVIGIQRQGRSY
                     PIVTTNFFDKASVQAVLDIEPGKEPGLYRLVIGPDDRPVSNAEVKYLWFRGQRNAQGG
                     FDRLMMAELVFSKGEWAEYVFLGKELAPRVNRAVLAGKYKDEKGGTWEFSDGGEAYWP
                     DKTFAYEISLNDLAANCEYLEAEDLRAPDAKLRYGYAWRGGKLLLYPARLADKSVRCG
                     PRPFATLTPQ"
     gene            complement(851..>1337)
                     /locus_tag="AC158Q_08245"
     CDS             complement(851..>1337)
                     /locus_tag="AC158Q_08245"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HIR3339807.1"
                     /translation="QVFRAWHFELSYGELLDSNRRLMKDTVVWNIEQGRALSGSRVGA
                     AERRRTALFHRMRQFMDNYDFLAVPVSQVLPFDVSQPYVTEIAGHKLDTYIDWMKSCY
                     WISVTGQPAVCVPCGFSAGGLPVGLQIVGRYRDDFGVLQLAHAFERATGFWQQKPGVA
                     A"
BASE COUNT          232 a          439 c          408 g          258 t
ORIGIN      
        1 atcttatact ttgccccgca gcgcggggct gtctactgcg gcgtgagggt cgcgaagggc
       61 cgggggccgc accgcaccga tttgtccgcc agccgggccg gatacagcag aagcttgccg
      121 ccccgccagg catatccgta acgcagtttt gcgtccgggg ccctcaagtc ctccgcttcc
      181 aggtactcgc agttggcggc gagatcattg agcgagattt cgtaggcgaa ggtcttgtcg
      241 ggccagtagg cttcgccgcc gtcggagaat tcccaggtgc cgcctttttc gtccttgtac
      301 ttaccggcca gcaccgcgcg gttgacgcgc ggcgcgagct ccttgccgag gaaaacgtac
      361 tcggcccact cgcccttgct gaacaccagc tccgccatca tcaggcggtc gaatccgccc
      421 tgagcgttgc gctggccgcg gaaccacagg tatttcacct cggcgttgga caccggccgg
      481 tcgtcgggcc cgatgaccag gcggtacagg cccggctcct tgccgggttc gatgtcgagc
      541 acggcctgca ccgacgcctt gtcgaagaag ttggttgtca cgatcggata ggaacgcccc
      601 tgccgctgga tcccgatcac caccggggga actttcctcg ccgtctggtg gggcatgcga
      661 ctcttttcca gcgcctccag atattgcccc ttgatccaga ttccctcgag ttcgcgcaaa
      721 tagactcggt ccggccgtcg gggctgagtc tgggcaatag acagcgaaga cgccagtaaa
      781 gccagcgccg ccatgaggca taacaaggcc ttgctcctgc tctgcattcg gtttcccatt
      841 cagttcgaag tcatgcggca acgccgggct tttgctgcca gaagcccgtt gcgcgctcga
      901 aggcgtgtgc gagctgcagc acgccgaagt catcgcgata gcggccaacg atttgcagtc
      961 ccaccggcag cccgccggcg ctgaacccgc agggcacgca gaccgctggc tgtcctgtca
     1021 cgctgatcca gtaacaggac ttcatccagt cgatgtaggt gtcgagcttg tgaccggcga
     1081 tctctgtcac gtagggctgc gacacgtcga acggcaggac ctgcgagacc ggcactgcca
     1141 ggaagtcgta attgtccatg aattggcgca tgcgatggaa aagcgcggtg cgcctgcgct
     1201 cggccgcgcc cactcgcgac ccgctgagcg cgcgcccctg ctcgatgttc catacgaccg
     1261 tgtccttcat cagcctgcgg ttgctatcca atagctcgcc gtaggacagc tcgaagtgcc
     1321 aggctcgaaa tacctgg
//
LOCUS       DBNGSG010000317         2456 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2141497, whole genome shotgun sequence.
ACCESSION   DBNGSG010000317 DBNGSG010000000
VERSION     DBNGSG010000317.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2141497"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..481)
                     /locus_tag="AC158Q_08250"
     CDS             complement(65..481)
                     /locus_tag="AC158Q_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR3339808.1"
                     /translation="MSALCEAVKTYGPLAGRILLALLFIASGWGKIGGFEGTVGYIAS
                     RGLPLPQVAAAGAIAVELVGGILLIAGWQARWAATALFLFLIPTTLIFHNFWAVDAAQ
                     QGTQQIQFMKNLCIMGGMLYVMAFGAGPLSLDNRKS"
     gene            complement(648..1454)
                     /locus_tag="AC158Q_08255"
     CDS             complement(648..1454)
                     /locus_tag="AC158Q_08255"
                     /inference="COORDINATES: protein motif:HMM:NF020893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HIR3339809.1"
                     /translation="MEEMTGLLTQHGLALVFVNVLLTQSGVPVPAVPILIIAGAFVTQ
                     GQIALAPLVLATVAASLIGDTLWYAAGRLYGYRILRTLCRVAIEPDSCVKQTETIFER
                     WGAPSLMIAKYVPGFSTIAPPLAGTMRVGLPAFLGYSTVAALLWSGLPIALGALFHTE
                     VERALEWLESMGTGAVAAIAAIVLFYVAVKSVQRYLLIRFLRMVRIGADELMQLMAKE
                     TKPVVLDVRSATARRLDPRGIPGAIWVDIAAPQAALVAVPPDRDVVVYCS"
     gene            complement(1621..2229)
                     /locus_tag="AC158Q_08260"
     CDS             complement(1621..2229)
                     /locus_tag="AC158Q_08260"
                     /inference="COORDINATES: protein motif:HMM:NF015823.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HIR3339810.1"
                     /translation="MKSAMRIALACSTAALIAGCLSSGERSYQRYYVLEDSGVHAAPA
                     KSPRASTLLVAPTTASGFYETQDIVYSRTPGTRAYYQYHAWVERPGRAIGELLVARLA
                     RGGAFKAITRATSGIQGELVLNTHLSEFYYDASAEPGSVRVSLTAELIDSVRRVLVAR
                     RTFTQTMPAATYDAPGAARALNQATVVVLDEVSAWVDRTAPR"
     gene            complement(2226..>2456)
                     /locus_tag="AC158Q_08265"
     CDS             complement(2226..>2456)
                     /locus_tag="AC158Q_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339811.1"
                     /translation="VREISASIRQVSADLARLSARADALFADGNIELRVTAQELRAAA
                     DSLGATARRFGDPGKVLFGPPAGSLGPGEAAK"
BASE COUNT          442 a          845 c          776 g          393 t
ORIGIN      
        1 gtgacgacaa tgccgtcggc aggataacgg tgaatcttga ccgtgctcat ggctgcccgt
       61 cccctcaact cttgcggttg tcgaggctca gcggccccgc gccgaacgcc atcacgtaca
      121 gcatgccgcc catgatgcag agattcttca tgaactggat ctgctgcgtg ccctgctgcg
      181 ccgcgtccac cgcccagaaa ttgtggaaga tgagggtggt cgggatcagg aacaggaaca
      241 gcgccgtcgc tgcccagcgc gcctgccagc cggcgatgag aaggatgccg ccgaccagct
      301 cgaccgcgat tgccccggcc gcggcgacct gcggcagcgg caggcccctg ctggcgatgt
      361 agccgaccgt gccctcgaag ccgccgatct ttccccagcc ggaggcgatg aaaagcagcg
      421 ccagcaggat gcgccccgcg agcggtccgt aggtcttcac tgcttcacac aatgcgctca
      481 tatgccctcc tcatgccatg gatgatggct cactgcgctg cttcgacaac ctcggtcacc
      541 ccgcccgcct cgatccagcc ttccagcccg ccttcgagcg ggcgcacgcg ccggtagccc
      601 cggtccatca gctcgcgggc gacacgcgcc gcgctggctt cgttgggtca actgcaatag
      661 acgaccacgt cgcggtcggg cggcaccgcg acgagagccg cctgcggcgc cgcgatatcc
      721 acccagattg cgccgggtat cccgcgcggg tcaagcctgc gcgccgtggc cgagcgcaca
      781 tcgagcacga ccggctttgt ctccttcgcc atcaactgca tcaattcgtc cgcgccgatc
      841 cgcaccatgc gcagaaaccg gatcagcagg taacgctgta ccgacttgac ggcgacgtaa
      901 aacagcacga tcgccgcgat cgccgccacc gcgccggtgc ccatgctttc cagccactcc
      961 agggcgcgct cgacctccgt gtggaagagt gcgccgagcg cgatcggcag ccccgaccac
     1021 aacagggcgg cgacggtgct gtagcccagg aaagccggga gcccgacccg catcgttccg
     1081 gccagcggcg gggcgatggt ggaaaatccc gggacgtact tggcgatcat caacgaggga
     1141 gcgccccagc gctcgaagat ggtctcggtc tgctttacgc aggaatccgg ctcgatggcg
     1201 acgcggcaca aggtgcgcag gatgcgatag ccgtagagcc ggccggctgc ataccacagc
     1261 gtgtctccga tcagcgaggc cgccacggtc gcgaggacga gcggcgccag cgcgatctgc
     1321 ccctgcgtga cgaatgcgcc ggcaatgatc aggatcggga ccgcgggaac cggcaccccc
     1381 gattgcgtca gcaaaacgtt gacgaacacc agcgcgaggc cgtgttgcgt cagcaatccc
     1441 gtcatctctt ccatggttca attcccggga aatagtaaac gatagccacg gaatcacgcg
     1501 gaatgacacg gacaatggct cgacccccgc gggcgccccg taagcattca agaattttct
     1561 gcgtggttcc gtgtgtttcc gtggctgaag cggttctcaa gatgacttcc agtcactcat
     1621 ttaacgcggt gccgtgcgat ccacccacgc cgacacttcg tcgagcacca cgaccgtggc
     1681 ctggttgagc gcccgcgccg ctcccggcgc atcgtacgtc gcggcgggca tcgtttgcgt
     1741 gaatgttcgc cgcgccacca gcacccgccg cacggaatcg atcaattccg cggtcaggga
     1801 aacccggacg ctgcccggct ccgctgacgc gtcgtagtag aactcggaga gatgggtatt
     1861 gagaaccagc tctccctgga tgccgctggt ggctctcgtg atcgccttga acgcaccgcc
     1921 acgcgcgagg cgcgcgacca ggagctcgcc gatagcgcgg ccgggccgtt ctacccaggc
     1981 atggtactgg taataggccc gcgtgcccgg agtgcggctg tatacgatgt cctgggtttc
     2041 gtagaatccc gatgcggtgg tcggagcgac caatagcgtc gatgcgcggg gcgattttgc
     2101 cggcgcggcg tgaacgcccg aatcctccag cacgtaatag cgctggtacg aacgctcacc
     2161 ggaagacagg catcccgcga tcagcgccgc ggtcgagcac gccagagcaa tccgcatggc
     2221 cgatttcatt tcgccgcctc ccccgggccc aggctgcctg ccggcggtcc gaacaggacc
     2281 ttgccggggt cgccgaaccg gcgtgcggtt gctcccagcg aatcggctgc cgcgcgcaac
     2341 tcctgcgccg tgacccgcaa ttcgatgttg ccgtcggcga acagggcgtc cgcgcgcgcc
     2401 gaaagccgcg cgagatcggc gctgacctgg cggatggagg cggaaatttc ccgtac
//
LOCUS       DBNGSG010000318         4044 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_953874, whole genome shotgun sequence.
ACCESSION   DBNGSG010000318 DBNGSG010000000
VERSION     DBNGSG010000318.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_953874"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1399
                     /locus_tag="AC158Q_08270"
     CDS             <1..1399
                     /locus_tag="AC158Q_08270"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3339812.1"
                     /translation="TLARFIVNHKYADVPQKVRHEAARSFLNWVGCAVGGARHETVGN
                     ALAALAPFSGPAEATVLGRGDRLDIMLAALMNGITSHTFDFDDTHLKTVIHPSGPVAS
                     AILALAERKPVRGEDFLHAFILGVEAECRIGNAVYPEHYDVGWHITGTAGVFGAAAAA
                     GRLLGLTEQQMTWALGIAATQSSGLREMFGTMCKPFHPGNAARNGLLAALLAKSNFTS
                     SLQGIEAKRGFGNVLSTGFRPGEITDRLGETWEISLNTYKPFACGIVEHPIIDGCIQL
                     RNAHKLRPEDIEGVSLKVHPLVLELTGKKTPQTGLEGKFSVYHSAAVGVIYGAAGEEQ
                     YSDAVVREPRVIALRDKVQATQEKGVHEDQARVAIRVRSGKVHETFIEHSVGSLDRPI
                     SDAGLEAKFRGLSAGILSKGESDNLIRLCWDVGKLKDAGEVARASVPGQKAKASTTTA
                     AAQAKKSAAAKGRAR"
     gene            1396..1983
                     /locus_tag="AC158Q_08275"
     CDS             1396..1983
                     /locus_tag="AC158Q_08275"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024809.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR3339813.1"
                     /translation="MKLYLTDNSGNSYKVRVLASILKVPYKKVYVDWEGKEHKQPAFL
                     KMNPRGQVPVMVIKGQAFWDSTAHLVYIARKYGGRKWLPTDALGLAEVSQWLAFAQNE
                     VYFGLQWARGVAKGLRGGNLEEYQGYGRKALDTLTWRLQEHDWLALNRPTIADIACYP
                     YVKRAPEGGLSLATYPAVSAWLKRCEKIPGWLKLD"
     gene            2147..3205
                     /locus_tag="AC158Q_08280"
     CDS             2147..3205
                     /locus_tag="AC158Q_08280"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HIR3339814.1"
                     /translation="MSLKIGILLGDDIGLEVVPEAVKVMKAAAAKTGLAIEWQDLPIG
                     KRGHEQHGHTMPPLTVDALAKTDGWVCGPIGHNAYPRNDPTWVMPPLRKKFELFANVK
                     PVKSYAGIPSVHKDVDIVFLRETTEGMMSSSVVFAGSGEFQPNDEIAIGMRVVTRKGA
                     NRVAREAFEVARTRRRKMVTALHKEPVYRLVCGMFARECRKVAQEYPDVQFNEVLIDG
                     FALKLVMKPQQFDVVVTTNQFGDIVTDEGAGLVGGLGLAPGLVVGEAQAMAQATHGSA
                     PDIAGRNIANPYAMIMSGKMLLEWLGRKRGEPKATQAAKLIDAAMDKVIAEARHLTGD
                     LGGKASTTEMGDAVAAAV"
     gene            3324..>4044
                     /locus_tag="AC158Q_08285"
     CDS             3324..>4044
                     /locus_tag="AC158Q_08285"
                     /inference="COORDINATES: protein motif:HMM:NF012407.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HIR3339815.1"
                     /translation="MLKIGILLGDDIGLEVVPEAVKVMKAAAKKAGLEIEWSDHPIGR
                     RGHELHGHTMPQATVAALEKTDGVLCGPIGHGAYPRNDPTWIMPPLRKRLDLYASIKP
                     VKSYPNIRSVHKNVDIVFLREVTEGMQSSDTVVAGAGEFRPNDEITIASRVITRKGSR
                     RVAKEAFEIARTRRRKTVTSVHKEPVYRLACGMFAEECRKLAKEYPDVTLNEVLVDGF
                     AMKLVMNPQQFDVVVTTNQFGD"
BASE COUNT          792 a         1257 c         1373 g          622 t
ORIGIN      
        1 gacgcttgca cgattcatcg tcaaccacaa atacgccgac gtgccgcaga aggtgcggca
       61 cgaggcggcg cgctcgttcc tgaactgggt cggctgcgcg gtcggcggcg cgcgccacga
      121 gacggtcggg aacgcgctcg cggcgctcgc gccgttctcc gggccggcgg aggcaaccgt
      181 gctggggcgc ggcgaccggc tcgacatcat gctcgcggcg ctcatgaacg gcatcacgtc
      241 gcacaccttc gatttcgacg acacgcatct caagacggtg atccatccca gcgggccggt
      301 ggcttccgcc atcctcgcgc tggccgagcg caaaccggtc aggggcgagg acttcctgca
      361 cgccttcatc ctgggcgtgg aggcggagtg ccgcatcggc aatgccgtct atcccgagca
      421 ttacgacgtc ggctggcaca tcaccggcac cgccggcgta ttcggtgccg ccgccgcagc
      481 gggcaggctg ctcgggctca ccgaacagca gatgacgtgg gcgctcggca tcgccgcgac
      541 ccagtcctcc ggcctgcgcg agatgttcgg caccatgtgc aagccgttcc atcccggcaa
      601 cgccgcccgc aacggcctgc tcgcggcgct gctcgcaaag agcaacttca ccagttccct
      661 ccagggcatc gaggcgaagc gcggcttcgg gaacgtgctg tccaccggct tcaggccggg
      721 agagatcacg gaccggctcg gcgagacctg ggaaatatcg ctcaacacct acaagccgtt
      781 tgcctgcggt atcgtcgagc accccatcat cgacggctgc atccagttgc gcaacgcgca
      841 caagctgaga ccggaggata tcgagggcgt ttcgctcaag gtgcacccgc tggtgctgga
      901 gctcacgggc aagaaaacgc cgcagaccgg cctcgaaggc aagttcagcg tctaccactc
      961 cgccgcagtt ggcgtgatct atggcgccgc gggggaggaa cagtacagcg atgccgtggt
     1021 gcgcgaaccc cgggtcatcg cgctgcggga caaggtccag gcgacccagg agaagggcgt
     1081 gcacgaggac caggcgcgcg tcgcgatccg ggtcaggagc ggcaaggtgc acgaaacgtt
     1141 catcgagcat tccgtgggca gcctcgaccg cccgatcagc gatgccggcc tcgaggcgaa
     1201 gttccgcggc ctgtccgcag gcatcctgtc gaagggcgag tccgataacc tgatccggct
     1261 gtgctgggac gtgggcaaac tgaaggacgc gggcgaggtc gcgcgggcgt cggttccggg
     1321 ccagaaggcg aaagcgagca cgacgacggc ggccgcgcag gcgaagaaga gcgcggcggc
     1381 caaaggacgg gcgcgatgaa actgtatctg acggacaact cgggcaactc gtacaaggtg
     1441 cgggtgctcg cttccatcct caaggtgccg tacaagaagg tgtacgtgga ctgggagggg
     1501 aaagagcaca agcagccggc ctttctcaag atgaacccgc gcggccaggt gccggtgatg
     1561 gtcatcaagg gccaggcctt ctgggattcg acggcgcacc tcgtttacat cgcgcgcaag
     1621 tacggcggca ggaaatggct gccgaccgac gcgctgggcc tggcggaagt ctcgcagtgg
     1681 ctcgccttcg cgcagaacga ggtgtatttc gggctgcaat gggcgcgcgg ggtcgcgaag
     1741 gggctgcgcg gcggcaatct cgaggagtac cagggttacg gccgcaaggc gctcgatacc
     1801 ctgacctggc ggctgcagga gcacgattgg ctcgcgctca accgcccgac catcgcggat
     1861 atcgcgtgct atccctacgt gaagcgcgcg ccggaaggcg gcctgtcgct cgccacctat
     1921 cccgcggtga gcgcgtggct caagcgctgc gagaagattc cgggctggct caagctcgat
     1981 taagaatgga caggattaac aggattcaat taaaaaacaa gaacgaaaac gctttatttg
     2041 ttcagtttca gttccaatct tgttaatcat gtaaatcctg tccatttgtt tttggcgggt
     2101 ttgcggcaaa tcgtcagtta atcctttcta ttttttgagg gtggaaatgt ccctgaagat
     2161 cggaatcctg ctgggcgacg acatcgggct ggaagtggtg cccgaggccg tcaaggtgat
     2221 gaaggccgct gcggcgaaga ccgggctcgc catcgagtgg caggacctgc cgatcggcaa
     2281 gcgcggccac gagcagcacg gccacacgat gccgccgctc accgtggacg cgctggcgaa
     2341 gacggacggc tgggtgtgcg gccccatcgg ccacaacgcc tatccgcgca acgaccccac
     2401 gtgggtcatg ccgccgctgc gcaagaagtt cgagctcttc gccaacgtga agccggtgaa
     2461 gtcctatgcc ggcattccct ccgtgcacaa ggacgtggac atcgtgttcc tgcgcgagac
     2521 caccgagggc atgatgtcct ccagcgtggt gttcgccggc agcggggaat tccagcccaa
     2581 cgacgagatc gcgatcggca tgcgcgtcgt cacccgcaag ggcgccaacc gcgtggcgcg
     2641 cgaggcgttc gaggttgccc gcacccggcg ccgcaagatg gtgacggcgc tgcacaagga
     2701 gccggtctac cggctggtgt gcggcatgtt cgcgcgcgag tgccgcaagg tcgcgcagga
     2761 atacccggac gtccagttca acgaggtgct gatcgacggc ttcgcgctga agctggtgat
     2821 gaagccgcag cagttcgacg tagtggtgac caccaaccag ttcggcgaca tcgtcacgga
     2881 cgagggggcg ggactggtcg gcggcctcgg gctcgcgccg ggactggtcg tgggcgaggc
     2941 gcaggcgatg gcgcaggcga cgcacggctc ggcgccggac attgccggaa gaaatattgc
     3001 caatccttac gccatgatca tgtcgggcaa gatgctgctc gagtggctcg gcaggaagcg
     3061 cggcgagccg aaagcgacgc aggcggcgaa gctgatcgac gccgcgatgg acaaggtcat
     3121 tgccgaggcc aggcatctca ccggggacct tggcggcaag gcgagcacta cggagatggg
     3181 cgacgccgtc gcggcggcgg tgtagccgcc gcctaacgaa gtcgaaagtc gaaagtcgaa
     3241 agtcgagagc cgagaggcag ttgcgaactg cgcaggactc atcacccatc acccatcact
     3301 catcacgcat ttggacgaac agcatgctga aaatcggaat cctgctgggc gacgacatcg
     3361 gcctggaggt cgtgcccgag gccgtcaagg tgatgaaagc ggcggcgaag aaggccggac
     3421 tcgagatcga gtggagcgat catccgatcg gcaggcgggg ccacgagctg cacggccaca
     3481 ccatgccgca ggccacggtg gcggcgctgg agaaaaccga cggtgtcctg tgcggcccga
     3541 tcgggcacgg cgcctacccg cgcaatgacc cgacctggat catgccgccg ctgcgaaaac
     3601 gcctcgacct ctacgcgagc atcaagccgg tcaagtccta tccgaacatc cggtcagtcc
     3661 acaagaacgt ggacatcgtg ttcctgcgcg aggtgaccga gggcatgcaa tcctccgata
     3721 ccgtggtcgc cggcgccggc gagttccgcc ccaacgacga gatcaccatt gcctcgcgcg
     3781 tcatcacgcg caagggctcg cggcgcgtcg cgaaagaggc attcgagatc gcacgcacgc
     3841 gccggcgcaa gaccgtcacc tcggtgcaca aggagccggt ttaccggctc gcctgcggca
     3901 tgttcgccga ggaatgccgc aagctcgcga aggagtaccc ggacgtcacg ctgaacgaag
     3961 tgctggtgga cggcttcgcg atgaagctgg tgatgaaccc gcagcagttc gacgtggtgg
     4021 tgaccaccaa ccagttcggc gaca
//
LOCUS       DBNGSG010000319         1605 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_953988, whole genome shotgun sequence.
ACCESSION   DBNGSG010000319 DBNGSG010000000
VERSION     DBNGSG010000319.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_953988"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1275
                     /locus_tag="AC158Q_08290"
     CDS             <1..1275
                     /locus_tag="AC158Q_08290"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HIR3339816.1"
                     /translation="YPKGFRVLTCSTSSPGKLSSILRLGIEKSHHGLVQKLRGLPKQW
                     QAEAVNYDPVFVETGPVFENVLSNGKVDLSTFPSPFWHEKDGGRYIGTGCSVVTKDFD
                     SDWVNVGTYRVQAHDRNHVGLDMVPGKHGAIQYDKTMKAGKPFPVVIVCGADPLGYLI
                     SGIEVPFGMCEYNYIGAILKEPVAVVKGQLTGLPFPAASEIVLEGWAQPGDVRTEGPF
                     GEFHGYYPGKASTAPAVTIERIYYRNAPIMVGSPPAKPPNDYSYSKAVMRSALLFDAL
                     EAAGVPDVKAVWAHEIGGARMFNVVAIKQRYAGHAKQAGHILNQCGVGAYMSRYSVVV
                     DEDIDPSNLQEVMWAVATRTDPVTDIDIIQRGMGSKNDPMYVAYAYKAPFSSKAIIDA
                     CRPFDHLHEFPEVAEASKELQEKVRKKWADLF"
     gene            1373..>1605
                     /locus_tag="AC158Q_08295"
     CDS             1373..>1605
                     /locus_tag="AC158Q_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3339817.1"
                     /translation="MTRILTTLLLLLPLAFPAGAQEYPARPIRLIVAFAPGGVVDTAA
                     RVLTSKMTERLGWQFVIDNRTGGGGLIAGNIAA"
BASE COUNT          317 a          476 c          527 g          285 t
ORIGIN      
        1 tatccgaaag gtttccgcgt ccttacctgc agcacgagca gccccggaaa actctcctcc
       61 atcctgcggc tcggcatcga gaaatcacac cacggcctgg tgcagaagct gcgcgggctg
      121 ccgaaacagt ggcaggccga ggcggtcaat tacgatcccg tattcgtcga gaccggtccg
      181 gttttcgaaa atgtgctcag taatgggaaa gtggacttgt ccactttccc atcccctttc
      241 tggcacgaga aagacggcgg gcgctatatc gggaccggct gcagcgtggt gacgaaagat
      301 ttcgattccg actgggtgaa cgtcggcact tatcgcgtcc aggctcacga ccgcaaccac
      361 gtcgggctcg acatggtgcc gggcaagcac ggcgcgattc aatatgacaa gaccatgaag
      421 gcgggcaagc cgttccccgt cgtcatcgtg tgcggcgccg atccccttgg ctacctgatt
      481 tccgggatcg aggtgccgtt cggcatgtgc gagtacaact acatcggcgc gatcctgaag
      541 gagccggtgg cggtcgtgaa ggggcagttg accggcctgc cgttcccggc agcgtccgag
      601 atcgtgctgg aaggctgggc gcaaccgggc gacgtgcgca ccgaggggcc gtttggcgag
      661 tttcacggct attaccccgg caaggcctcg accgcgccgg cggtcacgat cgaacgcatc
      721 tattaccgca acgccccgat catggtgggc agtcccccgg cgaaaccgcc caatgattat
      781 tcgtactcga aggcggtcat gcgctcggcg ctcctgttcg atgcgctcga agccgccggc
      841 gtgccggatg tgaaggcggt gtgggcccac gagatcggcg gggcgcgcat gttcaacgtg
      901 gtggcgatca agcagcgcta tgccgggcac gcgaagcagg ccgggcatat cctcaaccag
      961 tgcggcgtcg gcgcttacat gtcgcgctac tcggtggtcg tggacgagga tatcgacccg
     1021 tccaacctgc aggaagtcat gtgggcggtg gcgacgcgca ccgatccggt gacggacatc
     1081 gacatcatcc agcgcggcat gggctcgaag aacgatccga tgtacgtcgc ctacgcctac
     1141 aaggcgccgt tcagctcgaa ggcgatcatc gacgcgtgcc ggccgttcga ccacctgcac
     1201 gagtttcccg aggtggccga agcgagcaag gagttgcagg aaaaggtccg caagaagtgg
     1261 gcggaccttt tctaagggaa aaaggaaaaa aaggcagaag gcggcaggag atatgctgcc
     1321 gtaacacttt tgccttttgc ctcgtgactt gtgccttcaa ggagggggag agatgacgcg
     1381 catactgacg actttattgc tgttgctgcc gctggcattc ccggcgggcg cccaggaata
     1441 tcccgcccga cccatacgcc tgatcgtggc gtttgcgcca gggggcgtgg tggacacggc
     1501 ggcccgcgtt ctgaccagca agatgacgga gcgcctggga tggcagttcg tgatagacaa
     1561 ccggaccggc ggcggcggcc tgatcgccgg aaacatcgcc gcgaa
//
LOCUS       DBNGSG010000320        11798 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2651180, whole genome shotgun sequence.
ACCESSION   DBNGSG010000320 DBNGSG010000000
VERSION     DBNGSG010000320.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 11798)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11798)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11798
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2651180"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3389)
                     /locus_tag="AC158Q_08300"
     CDS             complement(<1..3389)
                     /locus_tag="AC158Q_08300"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3339818.1"
                     /translation="MAADSIPVSGSGFTFDDLYQREGLVKLDHSFLAGLARADAQLHE
                     QLARARAGPCALARKQESELLLALVPHLERFVVRLFGIEVEAATLAARHDELAPLYHT
                     KRNFVQRKAMHKFKPDEAEGLDGPALERELEGAFGEPFNEIAFARHVTKWQKNEAAHT
                     TQLDTALRYAAWAALSGAGKARHRHGVLFKAPAKLDPQRLVPLMTEESDGYRTHRLDP
                     EHLRRREGFKLTDPGTNLVGALDEAHYCIWCHEQGKDSCSKGLLEKGAVGKGTGSFKK
                     SPFGVTLAGCPLEEKISEFHKAKTEGHAIGALAIIIVDNPMVAATGHRICNDCMKSCI
                     YQKQEPVNIPQAETRTLKDVLELPWGFEIYSLLTRWNPLNLRTPYPKPASGKRVLVTG
                     MGPAGIALSHHLMNEGHAVAGIDGLKIEPLPSEISGVDEHGKRAPFRPVRDIRDLYES
                     LDRRVMAGFGGVAEYGITVRWDKNLLKVVRLLLERRSEFAVIGGVRFGGTVTAEEAFT
                     MGFDHVALAIGAGRPTVLDLPNGLARGVRAASDFLMALQLTGAAKPDSIANMQLRLPV
                     VVVGGGLTAIDTATESLAYYPLQVEKFLQRYERLAREHGETAARIAWNEEERDIADEW
                     LAHARAIRGERMRAVAEEREPRTLDLLQSWGGVTIAYRKRLIDSPSYTLNHEEVLKAL
                     EEGIRFAEGLTPLAIEVDKTNHARALKVSVQRRADDGRWEETGRAELPARTILIAAGT
                     QPNTVLAREDSTHFKLDGRYFSACDENGEPVAAEKAISKPVEARVLLSRRDDGRCISY
                     FGDVHPSYFGNVVKAIGSAKQGYPVVSRVLERVRPASGTGHREFLDRLNDQLRATVHE
                     VTRLTPLIVEVVVRAPLAASRFRPGQFYRLQNYETFAPRVEGSRLAMEGIALTGAWVD
                     AERGLISLITLEMGGSSDLCVLLKPGDPVVVRGPTGAPTEIPENETVILAGGGLGNAV
                     LFSIGQAMRRAGNRVLYFAAYKHMADRYKIPEIEAASDAVVWCCDEEPGFTPDRAQDK
                     SFVGNIVQGMHKYASGDLGEQPIPFGAADRIIAIGSDRMMAAVARARHDLLRPWLKPG
                     HLGIGSINSPMQCMMKEICAQCLQPHVDPQTGKVSYVFSCF"
     gene            3558..3704
                     /locus_tag="AC158Q_08305"
     CDS             3558..3704
                     /locus_tag="AC158Q_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339819.1"
                     /translation="MLNVRMTQWKRVLLWLILATLAALVTYFGIRGYLSPELLLNFSN
                     TFTC"
     gene            complement(3710..4594)
                     /gene="folD"
                     /locus_tag="AC158Q_08310"
     CDS             complement(3710..4594)
                     /gene="folD"
                     /locus_tag="AC158Q_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011325711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HIR3339820.1"
                     /translation="MSASSEAAPATMIDGAAAAREMISGLKERVATLRESNIRPGLAA
                     VQVGDNPASKIYLRNKVRACDEAGVRSEVHPLPADCPQGRVIDTLERLNGDPQVHGIL
                     LQLPLPHHLDSDRIMQCVAPEKDVDGLTRANLGALVTGHPAFEPCTPAGVIFLLDRAG
                     VALDGRHAVVVGRSAIVGKPMALMLMARSATVTICHSRTRGLPDHTRRADILVAALGR
                     PRFITADMVKPGAAVIDVGINRLADGRLAGDVDFAAVRAVAGWITPVPGGVGPMTVAM
                     LIANTVRAAEHSATARAV"
     gene            complement(4596..4904)
                     /gene="ftsB"
                     /locus_tag="AC158Q_08315"
     CDS             complement(4596..4904)
                     /gene="ftsB"
                     /locus_tag="AC158Q_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HIR3339821.1"
                     /translation="MRVLTLTLAALIVLIQYPLWLGKGSWFRVWEVDQQIRSQREANR
                     QLQERNRALEAEVRDLKVGLEAIEERARSELGMIRQDEIFFQVLEPAPAARAPGPSKP
                     "
     gene            complement(4911..6194)
                     /gene="eno"
                     /locus_tag="AC158Q_08320"
     CDS             complement(4911..6194)
                     /gene="eno"
                     /locus_tag="AC158Q_08320"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HIR3339822.1"
                     /translation="MSAIVDVIAREILDSRGNPTIEADVLLESGVLGRAAVPSGASTG
                     SREAIELRDGDDGRYGGKGVLKAVENVNTEICEAIIGVDATEQSFVDRTLIDLDGTEN
                     KSRLGANAVLAVSLAVAKAAAEESGLPLHRYLGGAGAMSMPVPMMNVINGGAHSDNGL
                     DMQEFMVVPVGFSSFRDALRCGAEVFHALKKLLADRRLSTAVGDEGGFAPRLPKHEAA
                     IKMILEAIEAAGYQPGEQVALALDCASTEFFEDGEYTLDAEGQSMTAEQFVDFLNAWV
                     EKYPIISIEDGMAEDDWDGWKLLTRRLGKKVQLVGDDLFVTNTRFLKQGIEKGVANSI
                     LIKVNQIGTLTETFAAIEMAKRSGYTSVVSHRSGETEDTTIADIAVASNALQIKCGSL
                     SRSDRLAKYNQLLRIEEDLGDTASYPGRGAFYQLR"
     gene            complement(6227..7063)
                     /gene="kdsA"
                     /locus_tag="AC158Q_08325"
     CDS             complement(6227..7063)
                     /gene="kdsA"
                     /locus_tag="AC158Q_08325"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HIR3339823.1"
                     /translation="MELCGFKAGLEQPLFLIAGPCVVESREMALDTAGELKEICTQVG
                     LNFIYKSSYDKANRTSIKSFRGIGMNKGLQILDEVRKKIRVPVLTDVHEIEEIEEVAA
                     AVDVLQTPAFLCRQTDFICAVAAAGKPVNIKKGQFLAPGDMRNVVDKAKSVSQRNNIL
                     VCERGVSFGYNNLVSDMRSLMIMRDTGCPVVFDATHSVQLPGGQGTSSGGQREYVPVL
                     ARAAVASGISGLFMETHPNPEKALSDGPNAWPLPLMKPLLETLKALDALVKARGFAED
                     QL"
     gene            complement(7146..8819)
                     /locus_tag="AC158Q_08330"
                     /pseudo
     CDS             complement(7146..8819)
                     /locus_tag="AC158Q_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673302.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
     gene            complement(8910..11303)
                     /gene="ppsA"
                     /locus_tag="AC158Q_08335"
                     /pseudo
     CDS             complement(8910..11303)
                     /gene="ppsA"
                     /locus_tag="AC158Q_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901747.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
     gene            11376..>11798
                     /locus_tag="AC158Q_08340"
     CDS             11376..>11798
                     /locus_tag="AC158Q_08340"
                     /inference="COORDINATES: protein motif:HMM:NF015575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HIR3339824.1"
                     /translation="MADPKRTAFFVSDRTGITAEMLGHSLLTQFDDVRFNEITLPFVD
                     SVEKAHDVVKQINQQGVTDGVRPLVISTLARTEIAQIIGQSKALFLDCFEIFISPLER
                     ELGIRASHAIGRTHNVSDFVNYHHRIESVNYTLSHDDGI"
BASE COUNT         2004 a         3886 c         3652 g         2256 t
ORIGIN      
        1 ttgaagcagg agaacacgta actcaccttg ccggtctgcg ggtccacgtg cggttgcagg
       61 cactgggcgc aaatttcctt catcatgcat tgcatcggtg agttgatcga gccgatgccc
      121 aggtggccgg gcttgagcca gggcctgagc agatcgtgcc gcgcgcgcgc aacggccgcc
      181 atcatgcggt ccgagccgat ggcaatgatg cgatccgccg ccccgaacgg tatcggctgc
      241 tcgcccaagt cgccgctcgc gtacttgtgc atgccctgca cgatgttgcc cacgaaggac
      301 ttgtcctgcg cccggtccgg cgtgaatccc ggctcctcgt cgcagcacca caccaccgcg
      361 tccgaggcgg cctcgatctc ggggatcttg tagcggtcgg ccatgtgctt gtaggcggcg
      421 aagtacagca cccggttgcc ggcgcggcgc atcgcctgcc cgatcgagaa cagcaccgcg
      481 ttgccgagtc cgccgccagc gagtatcacg gtctcgttct ccgggatttc ggtcggcgcc
      541 ccggtcggcc ccctcacgac gaccgggtcc ccgggcttga gcaacacgca taagtccgaa
      601 gagccgccca tctcgagcgt gatcagcgaa atcaggccgc gctcggcgtc cacccacgcg
      661 ccggtcagcg cgatgccttc catcgccagc cgcgaacctt cgacgcgcgg cgcgaatgtc
      721 tcgtaattct gcaggcggta gaactgcccc ggccggaaac ggcttgcggc aagcggcgcg
      781 cgcaccacga cttccacgat gagcggcgtc aggcgcgtca cctcgtgcac ggtggcgcgc
      841 aactggtcgt taaggcggtc gagaaactcc cggtgccccg tcccggacgc aggccgcacc
      901 cgttccagca cgcggctgac gacgggatag ccctgctttg cactgccgat cgccttcacg
      961 acgttgccga agtacgaggg gtgcacgtct ccgaagtagc tgatgcagcg gccgtcgtcg
     1021 cggcgcgaga gcagcacccg ggcttcaacc ggcttcgaga tcgctttttc ggcggcgacc
     1081 ggctcgccgt tttcgtcgca tgcgctgaaa tagcggccat cgagcttgaa gtgcgtggag
     1141 tcctcgcggg cgaggaccgt gttgggctgg gtgccggccg caatcaggat ggtcctggcc
     1201 ggcagctcgg cccggcccgt ctcttcccac cggccgtcgt cggcgcggcg ctggaccgat
     1261 accttcaggg cgcgggcatg attggtcttg tccacttcga tggcaagcgg ggtcagccct
     1321 tccgcgaagc ggatgccctc ctcgagcgcc ttaagcactt cctcgtgatt cagcgtgtag
     1381 gaagggctgt cgatcagccg cttgcggtag gcaatggtca cgccgcccca cgactgcagc
     1441 aggtccaggg tgcgcggctc gcgctcctcc gcgaccgcgc gcatgcgctc gccccgtatc
     1501 gcgcgcgcgt gggcgagcca ttcgtccgcg atatcgcgct cctcctcgtt ccaggcgatc
     1561 cgtgcggctg tctcgccatg ctcgcgtgca agccgctcgt agcgctgcag gaatttctcc
     1621 acctgcaggg gataataggc gagcgactcg gttgccgtgt cgatggcggt cagcccgccc
     1681 ccaaccacga ccaccggcag cctcaattgc atgttggcga tcgagtccgg cttcgccgcc
     1741 ccggtcaact ggagcgccat caggaaatcc gacgccgcac gcaccccgcg ggcgagcccg
     1801 ttgggcagat ccagcaccgt gggccggccg gcgccgatcg cgagcgccac gtggtcgaag
     1861 cccatggtga aagcctcttc ggccgtgacc gtgccgccga aacgcacgcc gccgatcacc
     1921 gcgaactcgc tgcggcgctc gagcagcagg cgcaccacct tgagcagatt cttgtcccac
     1981 cgcaccgtga tgccgtactc ggcaacgccg ccgaagccgg ccatgacgcg ccggtccagg
     2041 gactcgtaga gatcgcggat atcccgtacc gggcgaaacg gcgcgcgttt cccgtgctcg
     2101 tccacgcccg atatttcgga tgggagcggc tcgatcttga gcccatctat tccggcaacg
     2161 gcgtggccct cgttcatcag gtggtgcgag agcgcaatgc ccgccgggcc catcccggtc
     2221 accagcaccc gcttgccgct ggccggcttc ggatacggcg tgcgcaggtt gaggggattc
     2281 cagcgcgtga gcagcgaata gatctcgaac ccccacggca gttcgagcac gtctttcagc
     2341 gtgcgggtct cggcttgcgg aatgttgacc ggttcctgct tctggtagat gcacgatttc
     2401 atgcaatcgt tgcagatgcg gtgcccggtg gccgcgacca tcgggttgtc cacgatgatg
     2461 atcgcgagcg cgccgatggc gtggccctcg gtcttggcct tgtggaattc ggaaatcttc
     2521 tcttcaagcg ggcaaccggc gagcgtcacc ccgaacgggc tcttcttgaa cgagcccgtc
     2581 cctttcccca cggcgccttt ctcgagcagc cccttcgaac acgaatcctt gccctgctcg
     2641 tggcaccaga tgcagtaatg ggcttcatcg agcgccccga caaggttggt gccaggatcg
     2701 gtgagcttga aaccctcgcg cctacgcagg tgttcggggt cgagacggtg ggtcctgtaa
     2761 ccgtccgatt cctccgtcat cagcggcacc agtcgctgcg ggtccagctt ggccggggcc
     2821 ttgaacagca cgccatggcg gtgcctggct ttgcccgccc ccgacagggc cgcccatgcc
     2881 gcgtagcgca gtgcggtgtc gagttgcgtc gtgtgcgccg cttcgttctt ctgccatttg
     2941 gtgacatggc gcgcaaaggc aatttcgttg aatggctcgc caaatgcccc ctccagttcg
     3001 cgctcgaggg cggggccgtc caagccttcc gcttcatccg gcttgaactt gtgcatggcc
     3061 ttgcgctgca cgaagttccg cttggtgtgg tagagcggcg cgagctcgtc gtgccttgct
     3121 gccagtgtcg cggcttccac ctcgatgccg aacaggcgca cgacgaagcg ttcgaggtgc
     3181 ggcaccagcg ccagcagcaa ttcggattcc tgtttcctgg ccaacgcgca aggcccggcg
     3241 cgcgcgcgcg ccagctgctc gtgcagctgg gcgtcggccc gcgcgagccc ggcgaggaaa
     3301 ctgtggtcga gtttcaccag tccctcgcgc tggtagaggt cgtcgaaggt gaatcccgaa
     3361 ccgcttacag ggatggagtc agccgccaag gtaagccgta gatgattgaa aaacggtaaa
     3421 ttatatcaat cggttaccgg gagcctcata accgcgtgtt ctaacgatat gtgacacgcc
     3481 cggcgccatc gccagctcga tgcctgtgca ataatgcggt cgacccgtaa ccggaacact
     3541 cgttcgcgca aacggccatg ctcaacgtcc gcatgacgca atggaagcgc gtcctgttgt
     3601 ggttgatcct ggccacactg gctgcgctgg tgacctactt cgggatccgc ggttatctca
     3661 gtccggaatt gctgcttaat ttctccaata cctttacctg ctgagcgcac tagacggcgc
     3721 gcgccgtggc gctgtgttca gcggcgcgca cggtattggc gatgagcatg gcgaccgtca
     3781 tgggacccac tccgcccggc acgggcgtga tccagccggc gacggcgcgc accgccgcga
     3841 agtccacgtc gcctgcgagc ctgccatccg cgagccggtt gatgccgacg tcgatgacgg
     3901 ccgcacccgg ttttaccatg tccgcggtga taaaacgcgg ccggcccagc gcagccacca
     3961 ggatatccgc ccggcgcgtg tgatccggca gcccgcgggt gcggctgtgg cagatcgtca
     4021 ccgtcgcgct gcgcgccatc agcatgagcg ccatcggctt gccgacgata gcgctgcgtc
     4081 ccacgactac cgcgtgacgg ccgtcgaggg cgacgccggc acgatcgagc aggaagatca
     4141 cgccggcagg cgtgcacggc tcgaacgcgg gatggcccgt gaccagcgct cccaggttcg
     4201 cccgggtcag cccgtcgaca tccttttccg gcgcaacgca ttgcatgatg cggtcggagt
     4261 cgagatgatg cggcagcggc agttgcagca ggatgccgtg cacctgcgga tcgccgttga
     4321 gcctttccag ggtgtcgatc acccggccct gcgggcagtc ggccggcagt gggtgcacct
     4381 cggagcggac gccggcttca tcacacgccc gcaccttgtt gcgaagatag atcttcgagg
     4441 ccgggttgtc gcctacttgc accgcggcca gaccgggacg gatattcgat tccctgagcg
     4501 tggcaacgcg ctccttcaac ccggaaatca tttcgcgcgc tgccgccgcc ccgtcgatca
     4561 tggtggcggg tgccgcctcg cttgacgctg acatgtcagg gcttcgaggg tccgggcgca
     4621 cgcgcggccg gcgcgggctc cagcacctgg aagaagatct cgtcctgccg gatcatgccg
     4681 agctcgctgc gcgcgcgctc ctcgatcgcc tccagcccca ccttgagatc ccgcacctcg
     4741 gcttccagcg ccctgttgcg ctcctggagc tggcggttcg cttcgcgctg cgagcggatc
     4801 tgctggtcga cctcccagac cctgaaccag ctgcccttgc ccagccacag cggatactgg
     4861 atcagcacga tcaatgcagc gagggtcaag gtcagcactc tcacgccgat ctagcgcaat
     4921 tggtagaacg ccccgcggcc cggataactc gccgtatctc ccaggtcctc ctcgatgcgc
     4981 agcaactggt tgtacttggc gaggcggtcc gagcgggaca gcgagccgca cttgatctga
     5041 agcgcattgg aagcgacggc gatgtcggca atagtcgtgt cctcggtttc gcccgagcgg
     5101 tgcgacacga cggaggtata gcctgaacgc ttggccatct cgatggccgc gaatgtctcg
     5161 gtcagtgtgc cgatctggtt cactttgatc aggatcgaat tggccacgcc tttttcgatg
     5221 ccctgcttga ggaaccgtgt gttggtcacg aacaagtcgt cgcccaccag ctgcaccttc
     5281 ttgcccaggc gccgggtcag cagtttccat ccgtcccagt cgtcctccgc catcccgtcc
     5341 tcgatgctga tgatcgggta tttctccacc caggcattga gaaagtccac gaattgttcc
     5401 gcggtcatgg actgcccctc cgcgtcgagc gtgtactccc cgtcctcgaa gaactcggtg
     5461 ctggcgcagt cgagcgcgag cgctacctgc tcgcccggct ggtagccggc ggcttcgatc
     5521 gcctccagaa tcatcttgat cgccgcttcg tgcttgggca ggcgcggtgc gaagccacct
     5581 tcgtccccca ctgcggtcga gagtctgcga tcggccagca gtttcttcaa ggcatggaac
     5641 acctcggcgc cgcaacgcag cgcatcgcgg aacgaggaaa aacccaccgg caccaccatg
     5701 aactcctgca tgtcgaggcc gttatcagaa tgcgcgcccc cgttgatcac gttcatcatc
     5761 ggcaccggca tcgacattgc gcccgccccg cccaggtagc ggtgcagcgg cagtcccgat
     5821 tcctccgctg ccgccttggc cacggccagt gacacggcga gcactgcatt cgcgcccagc
     5881 cgcgacttgt tctcggtgcc gtcgagatcg atcagcgtcc tgtcgacgaa actctgctcg
     5941 gtggcgtcca cgccgatgat cgcctcgcag atctcggtat tgacgttttc gaccgctttg
     6001 agcacgccct tgccgccgta gcggccgtcg tctccgtcgc gcagctcgat ggcttcgcgg
     6061 ctgccggttg acgctcccga cggcactgcg gcgcgcccca gcacaccgga ttccagcagc
     6121 acgtcggcct cgatggtcgg attgccacgc gagtccagta tttccctcgc tatcacgtca
     6181 acgatcgcgc tcattgtttt tcctgttgtg atcgacggtt aaacggctac agctggtcct
     6241 cggcgaaccc gcgggccttg accagggcgt ccagggcctt gagcgtctcg agcagcggct
     6301 tcatgagcgg cagcggccag gcgttgggcc cgtcggatag tgctttctcg ggattgggat
     6361 gggtttccat gaacaaaccc gagatgccgc ttgccaccgc cgcgcgcgcc agcaccggga
     6421 cgtattcacg ctggcctccg ctggaggtgc cctgcccgcc gggcaactgg accgaatggg
     6481 tggcatcgaa caccaccggg cagccggtat ctcgcatgat catgagcgaa cgcatgtcgg
     6541 aaacaaggtt gttgtagccg aatgaaacgc cccgctcgca gaccagaatg ttgttccgct
     6601 gactcaccga tttggcctta tccacgacgt tcctcatgtc gcccggcgcg agaaactggc
     6661 ctttcttgat gttgaccggc ttgccggcgg cggccacggc acagatgaaa tcggtctgcc
     6721 ggcacaggaa tgcgggtgtt tgcagcacgt ccactgctgc cgccacctcc tcgatttcct
     6781 cgatctcgtg gacgtcggtc aggaccggga cccgaatttt tttcctgacc tcatctaaaa
     6841 tctgaaggcc tttattcatc cctatgcccc taaaggattt tattgaagtc ctgtttgcct
     6901 tgtcgtagga cgacttatag atgaaattga gccccacctg agtgcagatt tccttgagtt
     6961 ctccggcagt atcgagcgcc atttctctcg actcgaccac gcaggggccg gcgatgagaa
     7021 agagcggctg ctccaggccc gccttgaagc cgcacagctc gataacgccg cgggaggccg
     7081 gaacacggtg ccgcggcgcc ggcgccggtt gcgatcgtcg cgggcgggaa gtcatggggc
     7141 agctctcagg cgatgttttt cagtcgcccg gcatcgcccg ccaacccggc ctggtccggc
     7201 tgggcccggc tttgcgcggc ctgcacgaac gccttgaaca gcgggtgtcc gtggcgcggg
     7261 gtcgaggtga actccgggtg gaactggcag ccgacgaacc agggatgtcc gggcagctcg
     7321 accatctcgc atagcccgtc cttggccgat accccgctta ccttgagtcc cgcggccttg
     7381 atccggggca ggtaatgatt gttaacctcg tagcgatggc ggtgccgctc catgatgcgg
     7441 tcctggccgt aaatactgcg caccgcggaa tcggcctcga gcacgcactc ctggccaccc
     7501 aggcgcatgg tgccgccaag ttccgaaccg gcatcgcgcc gctcgacccg gccatcccgg
     7561 tcctgccact ctgtgatgag cgcaatcacg ggatgcgggg tgtcggaatc gaattcggtg
     7621 ctgtgggcgc cgacgaggcc tgccacgttg cgggcgaact cgattaccgc gagctgcatt
     7681 cagaggcaga tgcccaggta cgggacgcgg ttctcgcgcg cgtagcggat tgccctgatc
     7741 ttgccttcca ccccgcgctt gccgaacccg cccggcacca ggatcgcgtc catgctggcc
     7801 aggcatccgg tgccggtctt ctcgatgttc tcggaatcca tgtaatggat cttgaccctg
     7861 gccccggtgt gtatgccggc atgaatcagg gcctcggtca gcgacttgta ggattcagtg
     7921 aggtcgacgt acttgcccac cagcgcgatg ttgaccgtgc gcagcgggtg ctccagcgcg
     7981 tagaccagct tctcccaggc cacgagattg gcgggcggag gcttcaaccc cagcttgcgg
     8041 caaacgatgt cgtcgagatt ctgcgcgtgg agctccgaag gaatcttgta gatgctgtcg
     8101 acgtcgacgg ccgagatgac cgcttccacc tgcacgttgg tgaacagcgc gatcttcctg
     8161 cgctcgtccg cgggcacggg gcggtcggcg cggcacagca ggacgtcggg ctggatcccg
     8221 atctcccgca actccttgac ggaatgctgg gtgggcttgg tcttgatctc gcctgccgcc
     8281 gcgacgtacg gcaccagcgt cagatggatg aagcaggtat tctcgcgccc gagttcgatc
     8341 gccatctggc ggatcgcctc caggaacggc aacgactcga tgtcgcctac cgtgccaccg
     8401 acctcgacga tggtgacctc ggcctcgcct gccccgcggc ggatgaagtg cttgatctcg
     8461 tcggtgatat gcggaatgac ctgcacggtg ccgccgagat aatcgccgcg gcgttccttc
     8521 ttgatcacgc tctcgtagat ctggccggtg gtgaagttgt tgcgcttcga catcttgacg
     8581 tgcaggaaac gctcgtaatg gccgaggtcg agatcggttt cggcgccatc ctcggtcacg
     8641 aagacttcgc cgtgctggaa cgggctcatg gttcccggat ccacgttgag atagggatcc
     8701 agcttcacca tcgagatttt gaggccgcgg gactcgagaa tggcggccag ggaggcggca
     8761 gcgatgcctt tgcccaggga ggaaaccaca ccacctgtga caaagatgta ttgagtcatc
     8821 gcataagcca gcggtttggc tattttagcc agaaaccgtg ccgcgttcaa cgacttggtc
     8881 gcaaaaaaac tgccgcaagg gcagccatgc taggtcttgc gggaactgcc gcactctttc
     8941 gccaggtaca gccaggtttc gaccaccgtg tcgggattga gggacaggct ttcgataccc
     9001 tcctccacca gccacttggc gaggtccgga tggtcggaag gaccctgccc gcagatgccg
     9061 acgtacttgc cggccttacg gcacgcctgg atcgcgagat gcagcatctg cttcaccgcc
     9121 gggtcgcgtt cgtcgaactg ggccgcgatg atgccggaat cccggtcgag acccagcgtg
     9181 agttgagtca tgtcgttgga gccaatggaa aaaccgtcga aatgctcgag aaactggtcg
     9241 gccaggatcg cattggaggg aatctcgcac atcatgatgg tgcgcaggcc cttgtccccg
     9301 cgcttcaacc cattttccgc caggagctgg agtactttct ttgcgtcgtc caccgtgcgc
     9361 acaaagggca ccatgatttc gatgttggtc agccccatct cgtcgcgcac cttcctgagc
     9421 gcgcggcact cgagctcgaa gcagtcgcga aacgaaggat cgacatagcg ccccgcccca
     9481 cggaagccga gcatggggtt ttcctctcgc ggctcgtagc gcgtgccgcc ggtcaggctc
     9541 gaatactcgt tcgacttgaa atcagacagg cgcacgatca ccggcttcgg ccagaatgcc
     9601 gctcccagcg tcgaaatgcc ctccgccagc ttttccacgt agaacgagac cgggtcggga
     9661 tagccggcgc tgtgctcctc gacggcgacc cggacgtccg ccgggatatc gggataggcg
     9721 agcacggcct tggggtgcac cccgatcatg cgggcaatga tgaactcgag ccgcgccaac
     9781 cccactcctt cgttgggcag tcgctgaaat tcgaaggcca gctccggatt accgacgttc
     9841 atcgtgatct tcaccggcga tttcggcatg ctggagagcg ataaatcgat gacctcggtc
     9901 tcgagcatac cgcggtagac gaagccggtg tcgccctcgg cgcaagaaac ggtgacggga
     9961 cgcccgtctt tcaagacttt cgtggcatca ccgcaaccca cgactgccgg gatgccgagt
    10021 tcgcgcgcga tgatggcggc atggcaggtg cgaccgccac gattggtgac gatcgcagcc
    10081 gcgcgcttca tcaccggttc ccagtccggg tcggtcatgt ccgtgaccag tacatcgcct
    10141 tcgcgcacgc tcgacatttc ggcggctgtc ttgatgattc gcaccggtcc gctgccgatt
    10201 ttctgcccga tcgccctgcc ggtcgccagc acctcggatc gctgcttgag gcggtagcgt
    10261 cgcagcgtgt cgaccttttc gctggtcttc accgtttcgg ggcgcgcctg cagcacgtag
    10321 agcttgccgt cggcgccgtc ctttcaccac tcgatgtcca tcggacgccc gtaatgggct
    10381 tcgatgatca tcgcgtaacg ggacagttct tcgacttccg tgtcgctgat cgagaaccgc
    10441 cgccgttccg tttccggcac attgaccgtc ctgaccgatt gcccgtcctc ccgcccgtcc
    10501 gcatagacca tcttgatggc cttggagccc agcccgcggc gcaggatcgc gcgcttgccc
    10561 tcgcgcaagg cgcgcttgta aacatagaac tcgtcgggat tgacctgccc ctgcaccacg
    10621 gtctcgccca gcccgtaggc cgaagtgatg aagacgacct ggtcgaatcc ggactcggtg
    10681 tcgagcgtga acatcacgcc gctcgcaccc aagtcgctcc gtaccatgtg ctggatcccg
    10741 gcggacaaag cgatctcctg gtgcgtgtag cccttgtgca cgcggtagga gatggcgcgg
    10801 tcgttgtaga gcgaggcaaa aactttcttg atggcggcat aaaggggatc gaggccgcgg
    10861 atattcagaa atgtctcctg ctggcccgcg aaggaagcgt ccggaaggtc ctccgccgtt
    10921 gcggaggaac gcaccgcgaa cgacgcatcc gcacccgcgc catcggcgag ccggccgaaa
    10981 gctgtcgcaa tctcctcgcg caatcggccg gggaacgcct gcgccgagat ccagccgcgg
    11041 atctccagtc cggctttcgc gagagcggtc acgtcgttaa cgtccagccc gtcaatgcgc
    11101 cgggcaatgc gctcatccag cttgtcctgg gccaggaaat tccggtacgc ctcggccgtc
    11161 gtggcgaagc cgccgggaac ccgcacgccg gcgctgccga gctggctgat catttcgccg
    11221 agcgaggcat tcttgccgcc aacgcggggc acatcgctca tgcgcagcgc ggcgaagtca
    11281 agtatcagtg gagacatgct cattcctgca gggtgtggta atttgccggg ttcggaaagc
    11341 gcgttatttt accggaacac agcggcaccg ccgagatggc agatcccaag cgcacggcat
    11401 ttttcgtatc cgaccgcacc ggcattaccg ccgagatgct gggtcacagt ctattgaccc
    11461 agttcgatga cgtacgtttc aacgagatca ccctgccgtt cgtggattcg gtggaaaagg
    11521 cgcacgacgt cgtaaagcag atcaatcagc agggcgtaac ggacggggtg cgcccactcg
    11581 tgatcagcac ccttgcccgg actgaaatcg cgcagatcat cggccagtca aaggcgttgt
    11641 tcctcgactg cttcgagatc ttcatctccc ccttggagcg tgagctcggt atccgggcat
    11701 cacatgccat cggccggacc cacaatgtgt cggacttcgt caattaccat caccgcatcg
    11761 aatcggtgaa ctacacactg tcgcacgatg atggcatc
//
LOCUS       DBNGSG010000321         9582 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_444102, whole genome shotgun sequence.
ACCESSION   DBNGSG010000321 DBNGSG010000000
VERSION     DBNGSG010000321.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9582)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9582)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9582
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_444102"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..495)
                     /locus_tag="AC158Q_08345"
     CDS             complement(64..495)
                     /locus_tag="AC158Q_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339825.1"
                     /translation="MRPARRHWVWVPLAAVVFGATAWAAFAPVGSDSREQVFVIPKGT
                     WARRSAGEKLEVLPSEIRLTLGIKDILVMKNQDDVPQMFGPVLIMPGQSFRLPFGVAS
                     SNQFACTAHVSGTLTVTVQPAPAPGWDRLRWRAGAIVNRNG"
     gene            complement(503..964)
                     /locus_tag="AC158Q_08350"
     CDS             complement(503..964)
                     /locus_tag="AC158Q_08350"
                     /inference="COORDINATES: protein motif:HMM:NF016221.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HIR3339826.1"
                     /translation="MIAFMLRLLVLSVALPVTSLAYGQAGDAEMRVEGAWARRAPMME
                     GGGSKGGSGNGAVYAVLVNPGKQPDALVAAASDAAAAVEIHESYQDMGMMMMRPVKKI
                     EVPAGRKVEMKPGGFHIMLLGLKRDLKAGQVVNLTLQFQKTGKIPVKAEIR"
     gene            complement(984..1577)
                     /locus_tag="AC158Q_08355"
     CDS             complement(984..1577)
                     /locus_tag="AC158Q_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR3339827.1"
                     /translation="MALHHRLTALLALLVIAAAAGAAEGPGLKSGAFNPPRTAPDFSL
                     QGSDGSELKLSRYRGKVVALGFGYTFCPDVCPTTLVDLAAARKKLGADGEKFQVVYVT
                     VDPERDSVKQLRDYLAHFDPTFVGATGTPEQLAAVNKAYGILVSKKVYKGSQTAYLVH
                     HSSFVYLIDPQGKQRALVPFGRSADDIAHDVRALLKK"
     gene            complement(1594..2637)
                     /locus_tag="AC158Q_08360"
     CDS             complement(1594..2637)
                     /locus_tag="AC158Q_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3339828.1"
                     /translation="MRAANEMIARFPRRRQLLLGTAAALCPGVALPQTARGGSSPLQV
                     GGLPVTCNLTLPVACLASANDAKGVKGGSQIFEFSKYSGWPEIKESLMAGRIKAAYML
                     APLIMDLADKSIPVKVVSLGHRSGAVIMVRTDSPYKNFKSLAKKRVAIPSRFAVDLLY
                     LRNMLKAEGMTEKDVEIVEMAPPDMPAALYAKAVEAYATGEPFGAVAQRAGYARPLSM
                     TRDAWPNYICCVLTVHESLIRENRPLVQQLVNYVLGAGVWLDTVPANRTKGIEIAAGP
                     KFFNQDPNVLKFVMENPADRVTYGDLQLIKPEFEELLQLSIEAGTIKRRIPYEKYVDE
                     SFMKNAKPARIAL"
     gene            2832..3767
                     /locus_tag="AC158Q_08365"
     CDS             2832..3767
                     /locus_tag="AC158Q_08365"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HIR3339829.1"
                     /translation="MNELIEIRHLRYFLAVAETENFTRAAERLRVTQPSVSQQIAHLE
                     RALRTPLFRRIGKRVQLTEAGAAFRRSAEVVLRKLEDACGSVHDVAGLVIGHVDLGVI
                     PALHIAWVPRVLERMSRDYPGVTVGVQERSSSEIETELEAGRMDLGFGLVTRNSPNIR
                     YEHLFSEPFSLLVSDRNELAGRKAIDIEDLDGMRFVLLPNSFDMRRSANDVFRRARVR
                     PRVAFEIGNIDSVMATVARAGTPTLLPAIVLRGREALGLRAIPLAGKIRPIDFGLLWA
                     IASTASPAALALAAALKAVISGVAPSSKQDPTLRR"
     gene            complement(3998..5035)
                     /locus_tag="AC158Q_08370"
     CDS             complement(3998..5035)
                     /locus_tag="AC158Q_08370"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR3339830.1"
                     /translation="MIRSRAACCVLLAALAFTQAHAAQPKGESFNPGPHAIDVPSWFR
                     ETFLDFREDVREAAKDGKRLLVYFGQDGCPYCRELMRVSFRQKDIADKTRRHFHAVAI
                     NIWGDRETAWIDGKTLTEKALAARLKVQFTPTLLFFDEKGAVVLRINGYYPPHRLHAA
                     LDYVAGRHEGKVPFADFLAREAREPASGRLHDQLFFLKPPLDLGRPRKPAARPLAVLF
                     EQRQCAACDELHKYGFSDPPARALISKFDVARLELFGTERLITPRGKPLTAEQWGREL
                     NVAYTPTLVFFDAGGREVFRIEAYLKPFHLASSLDYVASGAHRKEPNFQRYIQARAER
                     IRAAGGKVDLW"
     gene            complement(5083..5673)
                     /locus_tag="AC158Q_08375"
     CDS             complement(5083..5673)
                     /locus_tag="AC158Q_08375"
                     /inference="COORDINATES: protein motif:HMM:NF014269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HIR3339831.1"
                     /translation="MPDPDQEVHIFPLNTVLFPDGLLPLKVFEQRYVEMTKACLSGNR
                     PFGVCLIREGREVGTAAVPEPVGCLAAIEHWDMPQLGVFQLVARGGGRFRIREMRVAT
                     NHLITATIEPLAPDDAAGTVDPLCREVLKAIIDKVGAERFSSPPRLDDAAWVGYRLAE
                     VLPLDTRVKQELLELMDAERRLERLHAVLVKQGLAA"
     gene            complement(5783..6619)
                     /gene="rapZ"
                     /locus_tag="AC158Q_08380"
     CDS             complement(5783..6619)
                     /gene="rapZ"
                     /locus_tag="AC158Q_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HIR3339832.1"
                     /translation="MQLILITGLSGSGKSVALNVLEDSGYNYVDNLPLKLLRQTVELL
                     AAAGRERAALSIDMRSAAELAELPGQLAEFRKRGHDVRVLFLEAKTETLIKRFSETRR
                     RHPLADDRLTLTECIERERELLADIAGLAHHVDTSELGPNTLRGWVRDFVGLPHAGLT
                     LLFESFGFKHGVPLNADFVFDVRCLPNPNYDPRLRPLTGRDGPVVEFLGSDPSARKML
                     EDIRRFVADWLPAFERDNRSYLTVAIGCTGGQHRSVYLVEALAGLFRDRAQALVRHRE
                     LS"
     gene            complement(6712..7644)
                     /gene="hprK"
                     /locus_tag="AC158Q_08385"
     CDS             complement(6712..7644)
                     /gene="hprK"
                     /locus_tag="AC158Q_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HIR3339833.1"
                     /translation="MPQVSIARLFEDNREKLRLEWVGGRQGGAKALNSELTKDSSQGL
                     IGHFNFIHPNLIQVLGASETAYLNGLDAASCRSLLAKAATRELACFIVAGAEHVPPPL
                     AEVAESSSTPLFRSPVASVELMWMLRPYLARALAESTTAHGVFLDVLGMGVLITGDSG
                     VGKSELALELVSRGSGLVADDVVELYRIGPETVEGRCPALLKDFLEVRGLGVLNIRTI
                     FGEAALRPRKNLKLIVRLGKPTEKMERLPLNAGSETVMGVDIRKVTIPVAAGRNLAVL
                     TEAAVRNYVLQLRGFDSTREFVGRQSQEMQKRES"
     gene            complement(7613..8098)
                     /gene="ptsN"
                     /locus_tag="AC158Q_08390"
     CDS             complement(7613..8098)
                     /gene="ptsN"
                     /locus_tag="AC158Q_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-carbohydrate
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HIR3339834.1"
                     /translation="MNLISKLLAPANVITDIDASSKKRVFEQVGLLFENNHQIARSQV
                     FDSLFAREKLGSTGLGQGVAIPHGRIKGLKDALGVLVRMKQPIPFDAPDGQAVSLIFV
                     LLVPDRATDLHLQILSELAQMFSDKAFRERLLAAGGAPELHQLVTQWQPHASGQHSPV
                     V"
     gene            complement(8138..8464)
                     /gene="hpf"
                     /locus_tag="AC158Q_08395"
     CDS             complement(8138..8464)
                     /gene="hpf"
                     /locus_tag="AC158Q_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation-promoting factor, HPF/YfiA
                     family"
                     /protein_id="HIR3339835.1"
                     /translation="MNLHLTGHHLEITPSIRDYVSTKLERIKHHFDHVIDVNVILTVE
                     KLQQKVEANVHVSGKDIFCESTANDMYAAIDGLMDKLDRTIIKHKEKSLGHRHDGSSL
                     KNAPGE"
     gene            complement(8484..>9582)
                     /gene="rpoN"
                     /locus_tag="AC158Q_08400"
     CDS             complement(8484..>9582)
                     /gene="rpoN"
                     /locus_tag="AC158Q_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011353225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HIR3339836.1"
                     /translation="QVAADAPTLRAHLLAQLSLTNLPERDRRLAALLIDSLDDDGYLT
                     QGLEELAALLPPELEVDPEELQIALRHVQNLDPAGVGARSLSECLALQLAVMPADTPH
                     LGAALETVKNHLEALAARDFTRLKKLLRCEDATLRSVQKVITGLNPKPGRNFFTEETR
                     YVVPDVIVKRVKGVWIASLNPDAMPKLRINRLYAEILQRNRNAGSQQLTSQLQEAKWL
                     IKNVQQRFDTILRVSQAIVDRQRHFFEHGEVAMRPLVLREIAETLDLHESTVSRVTTQ
                     KFMHTPRGIFELKYFFGSHVTTEAGGSASSIAIRALIKQLVSAENARKPLSDGQISEI
                     LSQQGIMVARRTVAKYRESMQILPVNLRKNL"
BASE COUNT         1641 a         3037 c         3114 g         1790 t
ORIGIN      
        1 gtcagcagcg acagcggggg aagaatttgc ctggctttcc gcacggtgaa gctgtccccg
       61 aggctacccg ttccgattca cgattgcgcc cgcccgccag cgaagccggt cccaaccggg
      121 ggccggcgcc ggctggacgg tcacggtcaa tgtgccgctc acgtgcgccg tgcacgcgaa
      181 ctgattgctg gacgcgacac cgaacggcag ccggaagctc tgcccgggca tgatcagcac
      241 cggcccgaac atctgcggca cgtcatcctg gttcttcatg acgagaatgt ccttgatgcc
      301 gagcgtcagc cggatttcgg agggcaatac ttcgagcttc tccccggcgc tcctgcgcgc
      361 ccatgttccc ttgggaatga cgaagacctg ctctctcgaa tcggagccta cgggagcgaa
      421 cgccgcccac gcggtcgcgc cgaaaacgac tgcggccagc ggaacccaga cccaatggcg
      481 tcgggcgggc ctcatcggcg catcacctga tttccgcctt taccgggatc tttccggttt
      541 tttgaaattg aagcgtgagg ttcaccacct gtccggcctt gaggtcgcgc ttgaggccca
      601 gcagcatgat gtgaaagccg ccgggcttca tctcgacctt cctgcccgcc gggacttcga
      661 tcttcttgac cgggcgcatc atcatcatgc ccatgtcctg gtaactctcg tgtatctcca
      721 ccgccgcggc ggcgtcgctt gcggcggcga ccagggcatc gggctgcttc cccgggttga
      781 ccagcacggc gtagacggcg ccattgccgc tcccgccctt cgatccgccg ccctccatca
      841 tcggcgcccg gcgcgcccag gccccttcca cccgcatttc cgcatcaccg gcctgcccgt
      901 acgccagtga cgtcacggga agggccacgg acaacaccag caatcgcaac atgaaagcaa
      961 tcattatgtt catttccttt cgttcatttt ttcagcaagg ccctgacatc gtgcgcgata
     1021 tcgtcggcgg agcgcccgaa cggcaccagc gcgcgctgct ttccttgagg atcgatcagg
     1081 tagacgaacg acgagtggtg gaccaggtag gccgtttggc ttcccttgta gactttcttg
     1141 gagacgagga tgccataggc cttgttcacc gctgcaagtt gctcgggcgt accggtcgcc
     1201 ccgacgaaag tcgggtcgaa gtgggcgaga taatcgcgca gctgcttcac gctgtccctt
     1261 tccggatcca cggtgacgta gacgacctgg aatttctcgc cgtccgcgcc cagtttcttg
     1321 cgggccgccg cgagatccac cagggtggtg gggcagacat cggggcagaa ggtgtagccg
     1381 aaacccagcg caaccacctt tccgcggtag cggctcagct tgagctccga gccgtccgat
     1441 ccctggaggg aaaagtcggg cgccgttcgg ggcggattga aggcgcctga cttgagcccg
     1501 gggccctccg cggcgcccgc ggcggccgcg ataacgagca gcgcaagcag tgcggtcaga
     1561 cgatgatgca atgccatgct tccaggcctg gagctaaagc gcaatgcggg caggctttgc
     1621 gtttttcatg aagctctcgt ccacgtactt ttcgtaaggg atgcggcgct tgatggtccc
     1681 ggcctcgatg gaaagctgca acaattcctc gaactcaggc ttgatcagct gcagatcgcc
     1741 gtaggtcacc cggtccgccg gattttccat cacgaacttg agcacgttcg ggtcctggtt
     1801 gaagaatttg ggcccggctg cgatctcgat ccccttggtg cggtttgccg ggacggtatc
     1861 cagccagacc ccggcgccga gcacgtagtt gacgagctgc tgcaccaggg ggcggttctc
     1921 cctgatcagc gattcatgca cggtcaggac gcagcagatg tagtttggcc acgcgtcgcg
     1981 cgtcatgctg agcggcctcg cgtagcccgc gcgctgcgcg accgccccga agggctcgcc
     2041 ggtggcatag gcttccaccg ccttggcgta gagggccgcc ggcatgtcgg gcggagccat
     2101 ctccacgatc tcgacatcct tttccgtcat tccttccgct ttcagcatgt tcctgagata
     2161 aagaagatcg acggcgaaac ggctggggat ggcgacccgc tttttcgcga gcgacttgaa
     2221 attcttgtac ggagagtcgg tccgcaccat gatgacggcg cccgagcggt gccccaggga
     2281 caccaccttg accggtatcg acttgtcggc gaggtccatg atcagtggcg ccagcatgta
     2341 ggcggccttg atccgtcccg ccatgagcga ttccttgatc tcgggccagc cgctgtactt
     2401 gctgaactca aaaatctggc tgccgccctt cacccctttt gcgtcattcg cgctggccag
     2461 gcaggcgacg ggcagcgtga ggttgcaggt gacgggcagg ccgcccactt gcagcgggct
     2521 gctgccgccg cgtgctgtct ggggcagcgc gacgccggga cataacgccg ccgccgttcc
     2581 aagcagcaac tgcctgcggc gagggaagcg tgcaatcatt tcgttggcgg cgcgcatctc
     2641 gtttctccct gtctatggtg gtcccgggcc ttccggcatg ggcgcgccga ttatacgtaa
     2701 aatcgccggt ctgccatagg catatcattt gatatcataa atattgctaa tatagaggca
     2761 gggcccgggc gagagccggt tcgcgcgggc gattgccgct cgatgccacg cagccagatg
     2821 aaagactcac catgaacgag ttgatcgaga tccggcacct gcgctacttt ctggcggtcg
     2881 ccgaaaccga gaatttcacc cgcgccgcgg aacggcttcg cgtcacgcaa ccgagcgttt
     2941 cccagcaaat cgcgcacctc gagcgcgccc tgcgcacgcc gctattccgg cgtatcggca
     3001 agcgggtgca actgacggag gcgggcgccg catttcgaag aagcgcggag gtcgtgctgc
     3061 gaaaactcga ggacgcctgc ggatccgtgc acgacgttgc gggcctcgtc atcgggcacg
     3121 tcgacctcgg cgtgattccc gcgctgcata tcgcatgggt gccgcgggtg ctcgagcgca
     3181 tgtcgcgcga ctatccgggc gttaccgtcg gcgtgcagga acgctcatcg agcgagatcg
     3241 aaacggaatt ggaggccggg cgcatggatc ttggatttgg cctcgtgacc cgcaactcgc
     3301 cgaatatccg ctacgagcac ctgttctccg agccgttctc gttgctggtt tcagaccgga
     3361 acgaattggc gggccgcaag gcgatcgaca tcgaggacct cgacggcatg cgcttcgtgt
     3421 tgctgcccaa cagcttcgac atgcgaaggt ccgcgaacga cgtcttccgg cgcgcgaggg
     3481 tgcgcccgcg ggtcgccttc gagatcggca acatcgactc cgtcatggcg accgtcgccc
     3541 gtgcgggaac gcctacgctc ctgccggcca ttgttctgcg cgggcgcgaa gcgctggggc
     3601 tgcgcgccat tccgctcgcg ggcaagatcc ggcccatcga cttcgggttg ctttgggcca
     3661 ttgcaagcac cgcgagccca gccgcgctgg cgctggcggc ggcgctgaag gcggtgatta
     3721 gcggagttgc gccgagctcg aagcaggacc cgaccctgcg ccggtgaggc gcatcctcgc
     3781 cgcgctatga aacgtcaata gacaggattg acagaagact tcatcgaaca cccgtcaaaa
     3841 ccacacatag acaggattaa caggatttac aagattaagg cacacaaagc gaaaagaggt
     3901 tgtagtttga atcctgttaa tcttgttaat cctgtccatt gacggggtta acgggtttac
     3961 gtctctacga ttgaatgttg ttaatcctgt ctgttcctta ccagaggtcg accttgccgc
     4021 ccgcggcgcg gattctctcc gcgcgcgcct ggatatagcg ctggaaattc ggctccttgc
     4081 ggtgcgcgcc gctggcgacg tagtccaggc tggaggcgag gtggaacggc ttgaggtagg
     4141 cttcgatgcg aaagacttcc cggccgccgg catcgaagaa cacgagggtc ggcgtatagg
     4201 ccacgttgag ttcgcgaccc cattgttccg cagtcagcgg tttgcctcgc ggcgtgatca
     4261 gccgctcggt gccgaataat tcgagccgcg ccacgtcgaa tttcgaaatc agcgcgcgcg
     4321 ccggggggtc gctgaacccg tacttgtgca gctcatcaca ggcagcgcac tgcctttgct
     4381 cgaacaacac ggcaagcggc cgcgcggcgg gcttgcgcgg ccgccccagg tcgagcggcg
     4441 gcttcaggaa aaagagctgg tcgtggaggc ggccgcttgc cggttcccgg gcctcgcgag
     4501 caaggaaatc ggcgaagggg accttgccct cgtgcctgcc ggcgacatag tccagcgccg
     4561 cgtggagccg gtgcggcggg tagtacccgt tgatgcgcag taccacggcc cctttctcat
     4621 cgaaaaacag cagagtcggc gtgaattgca ccttgagccg ggccgccagc gccttctcgg
     4681 tgagcgtctt gccatcaatc caggcggtct cgcgatcgcc ccagatgttg atggcgacgg
     4741 cgtggaagtg ccggcgcgtc ttgtccgcga tatctttctg cctgaagctg acgcgcatca
     4801 gctcgcggca gtacgggcag ccgtcctgtc cgaaatagac cagcaggcgc ttgccgtcct
     4861 tcgccgcctc gcgcacgtcc tcgcgaaagt ccaggaaggt ttccctgaac cacgaaggca
     4921 cgtcgatcgc gtgcgggccg gggttgaatg attcgccctt gggctgggcc gcgtgcgcct
     4981 gcgtgaaggc cagcgctgcc agcagcacgc agcacgcagc gcgcgatcga atcaacagaa
     5041 gcagaatcga atcacgactc acgaaacacg actcacgcat tctcaggccg ccaggccctg
     5101 tttcacgagc acggcgtgca gccgctcgag ccgccgctcc gcgtccatca gctcgagcag
     5161 ctcctgcttc acgcgcgtgt cgagcggcag cacctcggcc agccggtagc cgacccacgc
     5221 ggcatcatcg agcctgggcg gcgaggaaaa gcgctcggca ccgaccttgt cgatgatggc
     5281 cttgagcacc tcccggcaca acgggtccac cgtgcccgcc gcgtcatcgg gcgccagcgg
     5341 ttcgatcgtg gccgtgatca ggtgattggt cgcgacccgc atctcgcgga tgcggaagcg
     5401 cccgccgccg cgcgcgacca gctggaagac cccgagctgg ggcatgtccc aatgctcgat
     5461 ggcggcgagg cagccgaccg gttccggcac tgccgccgtt ccgacctcgc gcccctcccg
     5521 gatcaggcat acgccgaacg ggcggttgcc gctcaggcag gccttggtca tctcgacgta
     5581 gcgctgctcg aacaccttca gcggcagcag accgtccggg aacaggacgg tgttcagcgg
     5641 aaatatgtgg acttcctggt cggggtccgg catcacgttt cctgcctgac aaagcgtgat
     5701 tcgcgagtcg tgattcgtga gtcgagtaag cgctgcaacc gaagtctccg attcacgatt
     5761 caccaatcac gattcacgct cttcaagaga gctcgcggtg ccggaccagt gcctgggcgc
     5821 ggtcgcgaaa caggccggcc agggcctcga cgaggtagac ggagcggtgc tggccgccgg
     5881 tgcatccgat cgcgacggtg aggtaactgc ggttgtcgcg ctcgaaggcg ggcagccagt
     5941 cggccacgaa gcgccggata tcctccagca tcttccgcgc cgacgggtcg gaaccgagaa
     6001 attccaccac cggtccgtca cgcccggtca gcgggcgcag gcgcgggtcg tagttggggt
     6061 tgggcaggca gcgcacgtcg aacacgaaat cggcgttgag cggcacgccg tgcttgaagc
     6121 cgaaggattc gaacagcagg gtgaggccgg cgtgtggcag cccgacgaaa tccctgaccc
     6181 agccgcgcag cgtattggga ccgagctcgc tcgtgtccac gtggtgggcg aggccggcga
     6241 tgtcggccag cagctcgcgc tcgcgctcga tgcactcggt gagcgtcagc ctgtcgtcgg
     6301 ccagggggtg gcgccgccgt gtctcggaga agcgcttgat cagcgtctcg gtcttggcct
     6361 cgaggaacag gacccgcacg tcgtgaccgc gcttgcggaa ttccgcgagt tgcccgggca
     6421 gctcggcgag ctccgcggcg gagcgcatgt cgatgctcag ggccgcccgt tcccgcccgg
     6481 ccgcggccag caattccacc gtctgccgca acagcttcag cggcaggttg tccacgtaat
     6541 tgtagccgct gtcctcgagc acgttgagcg ccacgctctt gcccgagccg gagaggccgg
     6601 tgatgaggat cagctgcatt ctgccgaaag gcgtgattcg tgattcgtga tccgtgattc
     6661 gcatggcccg gcggcgggtc cagccaacaa agtccaatca cggctcacga ttcacgactc
     6721 gcgcttttgc atttcctggg actggcggcc gacgaattcc cgcgtgctgt cgaagccgcg
     6781 cagctggagc acgtaattgc gcactgccgc ctcggtgagc accgcgaggt tgcggcccgc
     6841 ggccacgggg atggtgacct tgcggatgtc cacgcccatg acggtctcgg agccggcgtt
     6901 gaggggcagc cgctccatct tctccgtcgg ctttccgagg cgcacgatga gcttcaagtt
     6961 cttgcggggg cgcagcgccg cctcgccgaa gatggtgcgg atattgagca cgccgagccc
     7021 cctcacttcc aggaaatcct tgagcagcgc ggggcagcgg ccttccactg tttcggggcc
     7081 gatgcgatag agctcgacga cgtcgtcggc gacgagtccg ctgccgcggc tcaccagctc
     7141 cagcgcgagc tcgctcttgc ccacgcccga gtccccggtg atgaggacgc ccatgccaag
     7201 cacgtcgaga aagacgccgt gggcggtggt gctttctgcc agcgcccgcg ccaggtaagg
     7261 gcgcagcatc cacatcagtt cgacgctggc caccggcgag cggaacagcg gagtggagga
     7321 agattcggcg acttccgcga gcggcggcgg tacgtgttcg gccccggcga caatgaagca
     7381 ggcgagttcc cgggtggcgg ccttggcgag cagcgaccgg cagctcgcgg catccaggcc
     7441 gttcagatac gccgtttcgg aagcgcccag cacctggatc aggttgggat gaatgaaatt
     7501 gaagtgaccg atcagcccct gcgaggaatc cttggtcagt tcgctgttca atgccttggc
     7561 gccgccctgg cggccgccga cccattccag gcggagcttc tcccggttgt cctcaaacaa
     7621 ccgggctatg ctgacctgag gcatgaggtt gccattgggt aaccagctgg tgcagctccg
     7681 gcgcgccccc ggccgccagc agccgctcgc ggaaggcctt gtcgctgaac atctgggcga
     7741 gctcggagag gatttgcagg tgaaggtcgg tggcgcggtc ggggaccagc agcacgaaaa
     7801 tcaggctgac cgcctggccg tccggcgcat cgaaggggat gggctgcttc atgcgcacca
     7861 gcaccccgag tgcgtccttc aggcccttga tgcgcccgtg cggaatcgcg acaccctgcc
     7921 cgaggccggt cgagcccagc ttctcgcgcg cgaacaggct gtcgaagacc tggctgcgcg
     7981 caatctggtg gttgttctcg aacagcagac cgacctgttc gaagacgcgt ttcttgctgc
     8041 tcgcgtcaat gtcggtgatg acgttcgccg gcgccagcag cttggaaatc aggttcatgt
     8101 cgcaatcctg gaatagtcgt gtgccgtcac gccgcagtca ctcgccgggc gcgttcttga
     8161 gcgaggagcc atcgtggcga tgcccgaggg atttttcctt gtgcttgatg atggtgcgat
     8221 cgagcttgtc catcaggccg tcgatcgcgg cgtacatgtc gttcgccgtg ctttcgcaaa
     8281 agatgtcctt gccgcttacg tggacgttgg cctcgacttt ctgttgcagc ttctcgaccg
     8341 tgaggatgac attgacgtcg atcacgtggt cgaagtgatg cttgatgcgc tcgagcttgg
     8401 tcgagacgta atcgcggatg gagggggtga tctcgagatg atgtccggtc aggtgcaggt
     8461 tcaatcgggg ctcctaggag tgcctaaagg ttctttctga ggttgacggg aagtatctgc
     8521 atggattcac ggtactttgc cacggtgcgg cgcgccacca tgatgccctg ctgggagagg
     8581 atttcggaaa tctggccgtc ggacagcggc ttgcgcgcgt tctccgccga caccagctgc
     8641 ttgatcagcg cccgtatcgc gatgctcgat gcggacccgc cggcctcggt ggtgacgtgg
     8701 ctgccgaaga agtacttgag ctcgaaaata ccgcgagggg tgtgcatgaa tttctgcgtc
     8761 gtgacgcgcg acaccgtgga ctcgtggaga tcgagcgtct ccgcgatctc gcgcaggacc
     8821 aggggccgca tcgccacttc gccgtgctcg aagaagtgcc gctgccggtc gacgatggct
     8881 tgcgacacgc gcaggatcgt gtcgaagcgc tgctgcacgt tcttgatgag ccacttggct
     8941 tcctgcagct ggctggtgag ctgttgcgat ccggcgttgc ggttgcgctg caggatttcc
     9001 gcgtagagcc ggttgatccg cagcttgggc atcgcgtccg ggttgagcga ggcgatccat
     9061 acccccttca ccctcttcac gatgacgtcg ggcacgacgt agcgcgtttc ctcggtgaaa
     9121 aagttgcgcc cgggcttggg gttgaggccg gtgatcacct tctgcaccga gcgcagcgtg
     9181 gcgtcctcgc agcgcagcag cttcttcagg cgggtgaaat cgcgcgctgc cagggcctcg
     9241 agatgatttt ttaccgtctc cagcgccgcg ccaagatgcg gagtgtccgc gggcatcacg
     9301 gcaagttgca gcgccaggca ttcggagagg cttctcgcgc ccacgccggc gggatcgagg
     9361 ttctgcacgt gcctcaacgc gatctgcaac tcctcggggt ccacttccag ctccggcggc
     9421 aggagggccg cgagctcttc cagtccctgc gtgagatagc cgtcgtcgtc cagggagtcg
     9481 atcagaaggg cggccagcct gcggtcacgc tcgggcaggt tggtgagcga caactgggcc
     9541 agcagatgcg cccgcagcgt gggcgcgtcc gcggccacct gc
//
LOCUS       DBNGSG010000322         8110 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_954616, whole genome shotgun sequence.
ACCESSION   DBNGSG010000322 DBNGSG010000000
VERSION     DBNGSG010000322.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 8110)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8110)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8110
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_954616"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1457)
                     /gene="ggt"
                     /locus_tag="AC158Q_08405"
     CDS             complement(<1..1457)
                     /gene="ggt"
                     /locus_tag="AC158Q_08405"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione gamma-glutamate
                     hydrolase [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIR3339837.1"
                     /translation="MLERGGNAFDAAVAAAAVLAVVEPYSSGLGGGGFFLLHRARDGR
                     QVMVDARETAPAGVRREMYLDPDGRPVRGATTRGGTAAGIPGVPAALVHVAGRYGALP
                     LAETLAPAIRHARDGFAVDGRYARIAGLRERFLQDLPEASRIFLEDSRAPRAGWRLRQ
                     PELAGTLERMARDGHDGFYAGPVARALVESVNRAGGAWRLSDLAGYRAIEREPLRIRY
                     RGATITTAALPSAGGVALVQCLGMLEHFSLADSRAPGSAHLVIEALRRSFHDRARYLG
                     DADFVAVPVERLASKDYSAQQAAAINPDAASRSEAPEHGPSPGSGNTTHLSVVDTAGN
                     RVAATLTINLLFGSGVVAGGTGILLNNEMDDFSLHPGLPNAFRLRGGDANAVAPGKRP
                     LSSMTPTFVEDGKGVIVLGAPGGSRIVSQVLLAVLDYLHAPAVNLPGIVNAPRYHHQW
                     WPDIVEIEPGAFTDGWRAELEARGHRIQLVKRRWG"
     gene            complement(1630..2193)
                     /locus_tag="AC158Q_08410"
     CDS             complement(1630..2193)
                     /locus_tag="AC158Q_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339838.1"
                     /translation="MRGDIVTQPTPANFSVCHGGTCAIVSQASLDEGQWRRIAAVFPA
                     PASNAEEERARIAEAIARFESIVGAITDTADDRAENQAGVNWWSQMDCIDESTNSTTY
                     LRILARAGLLIWHRVEARATRGYFLFGWPHTTAVLSEPSGARWTVDSWFHENGRPPEI
                     VPLESWKSGWRPAKDGLKPSMNADVRR"
     gene            complement(2303..3163)
                     /locus_tag="AC158Q_08415"
     CDS             complement(2303..3163)
                     /locus_tag="AC158Q_08415"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3339839.1"
                     /translation="MNRLCNAAGRKLARRLAAGVCLLAVMGGCTHQPASSDSALIDRA
                     IAGGHRSMADRARDIYRHPRETLLFFGLRPEMTVVEIWPGAGWYTEILAPVLRERGRY
                     YAAHFHVDEKSPRYMGPSRQNFEKRLADRAEIYDKTTVTAFSAPQQTAIAPRGTVDLV
                     LTFRNVHNWTAARNDEASFRAFYEALKPGGVLGVVEHRAQEGAPLEQMIKTGYMTEAY
                     VIALAERAGFRLVARSEINANPGDTRDHPRGVWTLPPTFRLGDQDRAKYAAIGESDRM
                     TLKFIKPASP"
     gene            3286..5043
                     /locus_tag="AC158Q_08420"
     CDS             3286..5043
                     /locus_tag="AC158Q_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR3339840.1"
                     /translation="MTAKENLSPIVHDAPVRSRESDEFWGSDVFAAMLRELDMPYISL
                     NPGASYRGLHDSIVNYLGNRTPQMLLSIHDENAVTIAHGYWKASDRMMAAALHSNVGL
                     MHATMSVFDAWCDRAPVLILGATGPWDAAKRRPWIDWVHTCSDQGALIRDYTKWDNQP
                     GSVEAGMEAMLRAAQIAQTAPRGPVYVNLDISLQMAKVGAMPALPDVKRYCAPDAVKP
                     STELVARAAKLLSGAKNPVMLAGRCLRGLDAWNQRVALAEKLNMRVLTNMKLAAAFPT
                     DHRLHAAPPANRFGPEALKMLAEADVILSLDWLDLGGLFKQAYGSKPVTAKIIQVSCD
                     QHSHRGWSMDYQALPPIDIYLMCEPEAAVPMLLEASKARAAGVPDRTSVKTPSVSGDA
                     VSMSGVAHALHAATKGTDVCYTHLPIGWSGAYSHFNHPLDYLGAEGGAGVGSGPGIAV
                     GAALALKGSGRLPIGVLGDGDFLMANTAVWTAAHYQIPCLIVVCNNRSFFNDELHQER
                     VAKERGRPPENRWIGMRIGDPDIDLAAMAKSQGAVGIGPVTKAGDLQKAIDQGVEVVA
                     KGGVCVVDARVLPGYDADG"
     gene            complement(5129..6151)
                     /locus_tag="AC158Q_08425"
     CDS             complement(5129..6151)
                     /locus_tag="AC158Q_08425"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HIR3339841.1"
                     /translation="MRILIYGAGAVGGFAGGILAAAGADVTLVARGAHHDAMKSRGLI
                     LEGKLNGRPEPIRVNVCKPGEEKPPYDFILVGLKSHQIAAAAKHMVSLLAKEGMILLG
                     QNGLPYWYFEKIDTPLRGSRLKSVDPEGTLASTIPIDAIIGGVMNKPADLVEPGRIRL
                     ADQPTDRLVIGELDNRVTPRLQAIKSLLDPAGWPVFITDNIRAAKWRKLLSNAVFNPL
                     GALTQSNARQIADFLPTQGIAKRMMDEVMAVATSVGVTPEMTPDQMMEDVLKRVGILS
                     STLQDVRFGRELELDAIVNAVIDVGRLTRVATPYLEFASACAGLLNRRIVDDGVAFAP
                     AAVKKA"
     gene            complement(6277..7386)
                     /locus_tag="AC158Q_08430"
     CDS             complement(6277..7386)
                     /locus_tag="AC158Q_08430"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIR3339842.1"
                     /translation="MAENVLAAVRTGPSRTEFREFPMPDVPADSALLKMEVAGICGTD
                     VKLYAHPPSGAPVIMGHENIGVIAKAGREFTRRKGFREGDLVFVEHYVMCGKCEWCHL
                     GQYRHCENTDWRTNPDGIRYGYTSAEKAPHLWGGFAQYVYLPWNAVVHAVPKGVTPEL
                     AGLVTPMANGVEWSLFDGGVGYNSTVLIQGPGQQGLSQTVICKQAGASLIVVTGTSRD
                     AARMKVARELGADHVIDVEQEDPLARIMEITGGKGVDVALDCTAGAGTLPILLGVNAL
                     KRKGGTIVVQGERKEFPNFPLEKVTVKFITIKSARGHSYRACELALAQLASKRFPLEK
                     VTTHRFGLKDVDLAIRSVGGKGVPDVIHASLMPWQ"
     gene            7431..7715
                     /locus_tag="AC158Q_08435"
     CDS             7431..7715
                     /locus_tag="AC158Q_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339843.1"
                     /translation="MKNEQRIQALARVKVWTRERFALPDAAAILVSEMACRLPGCPPL
                     ETHVLFLPPNGKRHHFKVFKPVTDVAPDDLPYAWLKDTLVLPDGAGCDCC"
     gene            complement(7766..>8110)
                     /locus_tag="AC158Q_08440"
     CDS             complement(7766..>8110)
                     /locus_tag="AC158Q_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339844.1"
                     /translation="LIGGDSGLRGYPSRYQSGTRRALMTVENRYYTDWFPFRLFRVGA
                     AAYIDHGRAWGGDNPNSGNPGWLTDIGFGLRIFSDRSATGRVLHIDLAFPLDADPAIR
                     SWQVIFKSRASF"
BASE COUNT         1373 a         2789 c         2569 g         1379 t
ORIGIN      
        1 ttgccccagc ggcgtttcac gagctggatg cggtgcccgc gcgcctcgag ttccgcgcgc
       61 cacccatccg tgaaggcccc cggctcgatc tccacgatat ccggccacca ctggtggtga
      121 tagcgcggcg cattcacgat ccccggcaaa ttcaccgccg gagcgtgcaa gtagtcgagc
      181 accgcgagca ggacctggct gacgatgcgc gaacccccgg gcgcgcccag cacgatcacg
      241 ccctttccgt cctccacgaa ggtcggcgtc atgctggaaa gggggcgttt tcccggcgcc
      301 acggcattgg catcgccgcc gcgcaggcga aaagcattgg gcaggcccgg gtgcagcgaa
      361 aaatcatcca tctcgttgtt gagcagaata cccgttcccc cggcaacgac tcccgaaccg
      421 aacagcaggt tgatcgtcag cgtggcggcg acgcgattgc cggcggtatc caccaccgag
      481 agatgcgtgg tgttcccgct gccaggcgac ggcccgtgct ccggcgcctc gctgcggctg
      541 gcggcatccg ggttgatggc cgcggcctgc tgcgctgagt aatctttgct ggcgagccgc
      601 tccaccggca ccgcgacgaa atcggcgtcc cccaggtaac gcgcgcgatc atgaaaggag
      661 cggcgcagcg cctcgatcac caggtgcgcg ctcccgggcg cgcggctgtc ggcgagactg
      721 aaatgctcca gcatgccgag acactgaacg agcgcgacgc cgcccgcgga aggtagggct
      781 gccgtggtaa tagtcgcgcc acgataccgg atgcgcagcg gctcacgctc gatcgcgcga
      841 tagcccgcga ggtcggatag tcgccatgcg ccccccgccc ggttcaccga ctcgacgagg
      901 gcgcgggcga cagggccggc atagaacccg tcgtgcccgt cccgcgccat tcgttcgagc
      961 gtgccggcga gctccggctg ccgcagccgc cagcccgcgc gcggcgcgcg gctatcttcg
     1021 agaaaaatgc gggaggcttc gggcaggtct tgcaggaacc gctcgcgcaa gccggcaatg
     1081 cgcgcataac gcccgtccac ggcaaaaccg tcgcgcgcat ggcggatcgc cggtgccagc
     1141 gtctcggcaa gcggcagcgc gccgtaacgt ccggcaacgt ggaccagcgc ggccggcacg
     1201 cccggtatgc ccgccgcggt accgccccgg gtcgtggcgc cgcggaccgg cctgccatcc
     1261 ggatccaggt acatctcccg tcttaccccg gccggcgcag tctcgcgcgc atccaccatt
     1321 acttggcggc catcccgcgc ccggtgcaga aggaagaaac ccccgccgcc cagcccggaa
     1381 gaataaggct cgactaccgc cagcacggcc gcggcggcga cggccgcgtc gaaggcattg
     1441 ccgccgcgct cgagcatttc gtgccccgcg gccgtggcga gtggatgagc tgaagcaatt
     1501 gccgaactat ttccttgcgc cgaagcgcac ggatacgaca gcaacgcggc aacaaaaacc
     1561 aggccccgcc acggcacgga tcgcgaaatg gacgcccctc ctggcttcgc ccctggaacc
     1621 ttgcccgatt tatcggcgta catcggcgtt catcgagggt ttcaagccgt ccttggccgg
     1681 ccgccatccc gacttccacg actcgagcgg cacgatctcg ggcggcctgc cgttctcgtg
     1741 aaaccaggaa tccaccgtcc acctggctcc cgacggctcg ctcagcacgg ccgtggtgtg
     1801 cggccagccg aacaggaagt atccgcgggt cgcgcgggcc tcgacgcggt gccatatcag
     1861 caggcccgcg cgggcaagaa tgcgcaggta cgtggtcgag ttggtggact cgtctatgca
     1921 gtccatctgg ctccaccagt tcacgccggc ctggttctcc gcgcggtcgt ctgcggtatc
     1981 cgtgatcgcc ccgacaatgc tctcgaaccg cgcgatggcc tcggcaatac gggcccgctc
     2041 ttcctccgca ttggacgcgg gggccggaaa caccgccgca atccgccgcc actgtccctc
     2101 atccaggctt gcctggctga cgatggcgca ggtgccgcca tggcacactg aaaaattcgc
     2161 cggcgtaggc tgggtcacaa tgtcgccgcg cacgaacgta tcagcggcgt accggtctgc
     2221 cgcgcccgcc tccgctgcga acagtaacgt cgcaacgcat aacgcaagcg tccttcgctt
     2281 cctgccgttc acggggcaac ggtcacgggc ttgccggctt gatgaacttg agcgtcatgc
     2341 gatcgctctc gccaatcgcg gcatacttcg cgcggtcctg atcccccagg cggaacgtcg
     2401 gaggcagcgt ccacactccg cgaggatggt ccctggtgtc tcccgggttg gcattgattt
     2461 cggagcgggc caccaacctg aagcccgctc tttcggccag cgcgattacg tacgcctcgg
     2521 tcatgtagcc ggtcttgatc atctgctcca gcggggcgcc ctcctgcgcg cggtgctcga
     2581 ccacgcccag gacgccgccc ggcttcagcg cttcgtagaa cgcgcgaaac gacgcttcat
     2641 cgttcctcgc ggcggtccag ttgtgcacgt tgcggaacgt cagcacgaga tccacggtcc
     2701 ccctgggcgc gatggccgtc tgctgcggcg cgctgaacgc ggtgaccgtc gtcttgtcgt
     2761 atatttccgc gcggtccgca agccgtttct cgaaattctg gcgcgacggc cccatgtagc
     2821 gcgggctttt ctcgtccacg tggaaatggg cggcgtaata cctgccccgc tcacgcaata
     2881 ccggcgcgag tatttccgtg taccagcccg cgcccggcca gatttccacc accgtcatct
     2941 ccggccgcag gccaaagaac agcagcgttt cacggggatg gcgatagatg tcgcgcgcac
     3001 gatcggccat cgagcggtgc ccgccggcga tcgcccggtc gatcaaggcg gaatcactac
     3061 tggccggctg atgggtgcag ccccccatca cggccagcag gcaaaccccc gcggccagac
     3121 gccttgccag tttgcgtcct gcggcgttgc ataagcgatt catcaacgcg gccccccgga
     3181 aaatcgctgt tatcatagca cccgaaattc acacgtccgc gtcactcatc gctgtttttt
     3241 agggtaagct acgccgtctt ttttcacctg tcaccgggaa caataatgac cgccaaggaa
     3301 aatctttccc ccatcgtcca cgacgcgcca gtgcgctcgc gggagagcga cgaattctgg
     3361 ggcagcgacg tatttgccgc catgctgcgc gaactcgaca tgccgtacat ctcgttgaat
     3421 cccggcgcga gttaccgcgg gctgcacgac agcatcgtca attatctcgg caaccgcacg
     3481 ccgcagatgc tgctctcgat ccacgatgag aacgcggtca ccatcgcgca cggctactgg
     3541 aaggcttccg accggatgat ggccgcggcg ctgcactcca acgtcgggct gatgcacgct
     3601 acgatgtcgg tctttgacgc ctggtgcgac cgcgcgccgg tgctgatcct gggcgcgacc
     3661 ggcccctggg acgccgcgaa gcgccgcccc tggatcgact gggtccatac ctgctccgac
     3721 cagggcgcgc tgatccgcga ctacaccaag tgggataacc agccaggctc ggtggaagcg
     3781 ggaatggaag cgatgctgcg cgcagcgcag atcgcgcaga cggctccgcg cggcccggtg
     3841 tacgtgaacc tcgacatttc cctgcaaatg gccaaggtcg gcgcgatgcc ggccctgccg
     3901 gacgtgaagc gctactgcgc cccggacgcg gtcaaaccgt cgaccgagct ggtcgctcgc
     3961 gcggcaaagc tgctgtcagg cgccaagaac ccggtaatgc tcgctggccg ctgcctgcgc
     4021 ggcctggatg cctggaacca gcgcgtcgcg ctcgccgaga agctcaatat gcgcgtgctg
     4081 accaacatga agctcgccgc tgccttcccg accgaccacc gcctgcatgc cgcaccgccg
     4141 gcgaaccggt tcggtccgga agcgctgaaa atgctggccg aggcggacgt gatcctgtcg
     4201 ctcgactggc tcgatctggg cggtctcttc aagcaggcgt acggttcgaa gccggtcacg
     4261 gcgaaaatca tccaggtttc ctgcgaccag cacagccacc gcggctggag catggactac
     4321 caggcactgc ccccgatcga catctacctc atgtgcgagc cagaagccgc cgtgccgatg
     4381 ctgctggaag catcgaaagc acgcgccgcg ggcgtgccgg acaggacctc ggtcaagacg
     4441 ccgtcggttt cgggcgacgc ggtttcgatg agcggcgtcg cgcatgcgct tcatgctgca
     4501 acgaaaggca ctgacgtctg ctatacccac ctcccgatcg gctggagcgg cgcctacagc
     4561 cacttcaacc atcccctgga ttatctcggc gccgagggcg gcgccggggt gggctcggga
     4621 ccgggcatcg cggtgggcgc ggcgctggcg ttgaaaggca gcggacggct gccgatcggc
     4681 gtgctcggtg acggcgattt cctgatggcc aataccgcgg tgtggaccgc ggcgcactac
     4741 cagattccgt gcctgatcgt ggtctgcaac aaccgctcgt tcttcaacga cgagctgcac
     4801 caggagcgcg tcgccaagga acgcggccgg ccgccggaga atcgctggat cggcatgcgc
     4861 atcggcgatc ccgacatcga cctcgcggcg atggcgaaat cgcagggcgc ggtcggcatc
     4921 ggtcccgtca ccaaggcggg cgacctgcag aaagccatcg accagggcgt cgaagtcgtc
     4981 gccaagggcg gcgtgtgcgt cgtggacgct cgggtgctgc cgggctacga cgccgatggt
     5041 tgaggaatag acaggattaa caggatttac aagattgaaa caacctcagc cacgaggtta
     5101 gtcctgtcaa tcatgtaaat cctgtccatt acgccttttt tacagcggcg ggcgcgaagg
     5161 caacgccgtc gtccacgatg cgccggttga gcagcccggc gcaggccgaa gcgaactcca
     5221 ggtaaggcgt cgcgacgcgg gtcaggcggc ccacgtcgat caccgcattg actatggcgt
     5281 cgagctccag ctcgcgcccg aaccgcacgt cctgcagggt cgaggacagg atccccaccc
     5341 gcttcagcac gtcctccatc atctgatccg gggtcatctc gggcgtgacg ccgaccgatg
     5401 tggccactgc catcacttcg tccatcatcc gcttcgcgat gccctgggtc ggcaggaaat
     5461 ccgcgatctg gcgcgcgttc gattgcgtaa gtgcgccgag cgggttgaac acggcgttcg
     5521 acagcagctt tctccacttg gccgcccgga tgttgtccgt gatgaacacg ggccagccgg
     5581 cgggatcgag caacgacttg atcgcctgga gccgcggcgt gacgcggttg tcgagctcgc
     5641 cgatgacgag gcggtcggtg ggctggtcgg cgaggcggat gcggcccggt tcgacgaggt
     5701 ccgccggctt gttcatgacg ccgccgatga tcgcgtcaat gggaatcgtg cttgcgagcg
     5761 tgccctccgg atcgaccgac ttcaggcgcg agccgcgcag cggcgtatcg atcttctcga
     5821 aataccagta cggcagcccg ttctggccga gcaggatcat gccctccttc gcaagcaggc
     5881 tcaccatgtg cttcgcggcc gccgcgatct ggtgcgattt cagcccgacg aggatgaaat
     5941 cgtaaggtgg cttctcctcc cccggcttgc aaacgttgac acggatgggt tccggccgcc
     6001 cattcaactt gccttcgagg atcagccccc ggctcttcat ggcatcgtgg tgcgcgccac
     6061 gggcaacgag cgtcacgtcc gcgcccgccg ccgccaggat gccgccggca aatccgccga
     6121 ccgcgccggc gccatagatc agtatccgca ttgcttctcc gaaaagtgga tggttatcgg
     6181 tgacgcagtg atacagtgat gcagtgacgc ggtgacggcg ctgcccgtgg gctttcgtca
     6241 cttcgtcact gtctcacttc acatcactgc agtttcttat tgccagggca tcagcgacgc
     6301 gtgaatgacg tccggcacgc ccttgccgcc caccgagcgg atggcaaggt cgacatcctt
     6361 cagcccgaac ctgtgggtcg tcaccttctc cagcgggaag cgcttcgagg ccagttgcgc
     6421 cagcgcgagc tcgcacgccc ggtagctgtg gccgcgcgcg cttttgatgg tgatgaactt
     6481 caccgtgacc ttctcgagcg ggaagttcgg gaactccttc ctctcgccct ggaccacgat
     6541 cgtgcccccc ttgcgcttca gggcgttgac gccaagcagg atcgggaggg tccccgctcc
     6601 cgcggtgcag tcgagcgcaa cgtccacgcc cttgccgccc gtgatctcca tgatccgcgc
     6661 gagcggatcc tcctgctcca cgtcgatcac gtggtcggcg ccaagctccc tggcgacctt
     6721 catccgcgcg gcatccctgg acgtgcccgt gacgacgatg agggatgcgc ccgcctgctt
     6781 gcagatgacg gtctgggaaa gtccttgctg gccgggcccc tggatcaata cagtcgagtt
     6841 gtagccgaca ccgccgtcga acagcgacca ctcgacgccg tttgccatgg gcgtcaccag
     6901 tcccgccagt tccggcgtga cgcctttcgg gacggcatga acgacggcgt tccagggaag
     6961 gtagacgtac tgggcgaatc cgccccatag atggggcgcc ttctcggccg aagtgtagcc
     7021 gtaacgaatg ccgtcgggat tggtgcgcca gtcggtgttc tcgcagtgac ggtactggcc
     7081 gaggtggcac cattcgcact tgccgcacat gacgtaatgc tcgacgaaca ccaggtcgcc
     7141 ttccctgaag cccttgcgcc gggtgaactc gcgtcccgcc ttggcgatga ccccgatgtt
     7201 ctcgtgtccc atgatcaccg gcgcgccgga cggcggatgc gcgtagagct tgacgtcggt
     7261 gccgcagatt cccgccacct ccatcttcag cagcgcgctg tccgccggca cgtccggcat
     7321 cgggaactcc cggaattccg tccggctcgg gccggtccgc acggccgcca gcacattttc
     7381 cgccacggcg ctatcctccg ctcaatgatt gcgtcggccg ccattctcgc atgaagaacg
     7441 agcagcgtat ccaggcgctt gcccgtgtca aagtgtggac gcgggaacgt ttcgcgcttc
     7501 ccgacgcggc cgccatactg gtgtcggaaa tggcgtgtcg cctgccgggc tgcccgccac
     7561 tcgagaccca cgtgctgttc ctgccaccga acggaaaacg tcaccacttc aaggtgttca
     7621 agccggtgac ggatgtcgcc ccggacgatc tcccctacgc ctggctcaag gacacgctgg
     7681 tcctgcccga cggcgccggt tgcgactgct gctgaccgtt gccgggacgc gggtcgtcgg
     7741 atccccgcgg ccacgacagg cgtgctcaga agctcgcccg cgacttgaag atcacctgcc
     7801 acgaccggat ggcgggatcg gcatcgagcg ggaatgccag gtcgatgtgc agcacgcggc
     7861 cggtagccga acggtcgctg aagatgcgca acccgaagcc gatgtcggtg agccatccgg
     7921 ggttgccgct gttcggattg tcgccgcccc atgcgcggcc gtgatcgatg taggccgccg
     7981 cccccacccg gaacaggcgg aacgggaacc aatccgtgta gtagcggttc tccacggtca
     8041 tcagcgcccg ccgtgtgccg ctctggtagc ggctcgggta gccgcgcagg ccggagtcgc
     8101 cgccgatcag
//
LOCUS       DBNGSG010000323         4097 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1633048, whole genome shotgun sequence.
ACCESSION   DBNGSG010000323 DBNGSG010000000
VERSION     DBNGSG010000323.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1633048"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="AC158Q_08445"
     CDS             <1..326
                     /locus_tag="AC158Q_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011614944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding enzyme"
                     /protein_id="HIR3339845.1"
                     /translation="RGENISGPELDRVIGEHPAVHEAAAIAVASELGEDEILVAVTLK
                     PGQSCSAQDIAAWSRQHLAPQKAPRYVVFVDELPHTPTHKVQKAVLRADATLKARAVD
                     LQANR"
     gene            complement(584..1171)
                     /locus_tag="AC158Q_08450"
     CDS             complement(584..1171)
                     /locus_tag="AC158Q_08450"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3339846.1"
                     /translation="MGDIRRAFVFLSLAVAAGSAMAQSRGDAARGETKAKACAACHGE
                     PGRAPLPLTPWLAGQLEEVTVLQLILIREGLREVPQMAGMLDKATDPDLMDIAAYYAR
                     QAPPRASSGGDAGLRARGAGLARSMGCGSCHLDDYSGQKQVPRLANQREDYLAATLKS
                     YRDNKRTGPDTSMNGIMYRVSDGDIEALAHFLAHQ"
     gene            complement(1199..2470)
                     /locus_tag="AC158Q_08455"
     CDS             complement(1199..2470)
                     /locus_tag="AC158Q_08455"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR3339847.1"
                     /translation="MNSFLQSCLRSTPLKAFVSCALGLAGAYALAQQLVGPQTKPPSA
                     KEVEARKSKGGYFPERMKNPNLSAHPAKMTVTPPSEIPLDRIKVPKGFKVELWAHGMP
                     GVRMMARGDKGTIWAGTRIIGRVYEIKDKGGERTHRILAQKLTQPNGVAYLAGSLYVA
                     AINRVFRYDGIEDDPNVKPVELTAQFKLPPDVHHNWKFMAFGPDSKLYIQVGSPCNIC
                     EPSDQHGQIRRYVADGSNMEVVARGVRNSVGFDFHPKTGELWFTDNGRDWQAENGPQD
                     ELNRVSKIGQFFGFPYCHANGIPDMDFKKKDPCKGVTLPVALMGPHAAALGMRFYTGG
                     MFPKEYQNSIFVARRGSWNRSSLFGFDVANVRVGADGKGAKVTPFMTGFMDPKENKFW
                     GRPVDVLQLPDGSLLVSDEQVGAIYRVTYQK"
     gene            2633..3643
                     /locus_tag="AC158Q_08460"
     CDS             2633..3643
                     /locus_tag="AC158Q_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3339848.1"
                     /translation="MNSHGNFRFWTGAFMALAMLGAPPAGLAQGPSTGSGQAYPNKPV
                     RLVLPYPPGGGSDTIARPLAQKMSEGLGQQVVVENRGGANGNIAMEYVARSAPDGYTF
                     VFGLNAQFAINPGLYRKIAYDPVRDFAPISLFGSGVYILVVHPSLPVKTVRELIVLAK
                     SRPGQIAYSSSGNGSGGHLAAELLNSMAGIRMLHVPYKGGGPALVDLIAGQVQVLFST
                     QLAGWPHVQSGRVRALAVSAAKRPSSIPNLPTLAEAGVSGYDAVPWYGVLAPAGTPRE
                     VVARLHNEIVRALNQPDFRGFLVNNGVEPIGGPPEDLARAIKADLVKWAKVIKEAGVR
                     VD"
     gene            3818..>4097
                     /locus_tag="AC158Q_08465"
     CDS             3818..>4097
                     /locus_tag="AC158Q_08465"
                     /inference="COORDINATES: protein motif:HMM:NF013567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3339849.1"
                     /translation="MRARLASEHPGVKFLAVSDREPARARELAQKTGAQFHSGDNAAV
                     ISRREVNAVIVSSSEGAHVEPILQAIELGKTVLVEKPIALSLADGDRII"
BASE COUNT          705 a         1306 c         1345 g          741 t
ORIGIN      
        1 gccgcggcga gaacatctcg gggcccgagc tcgaccgcgt gatcggcgag catcccgcgg
       61 tgcacgaagc ggcggccatc gcggtggcga gcgagctggg cgaggacgag atcctcgtcg
      121 ccgtgaccct gaagcccggg cagtcctgct cggcgcaaga catcgccgcg tggtcgcggc
      181 agcacctcgc gccgcaaaag gcgccgcgtt acgtcgtgtt cgtggacgag ctgccgcaca
      241 cgcccacgca caaggtgcag aaggccgtgc tccgcgccga cgcgacgctc aaggccaggg
      301 ccgtcgacct tcaggccaat cgctgacagt tacaaggttc aagaatcaag gtacacggta
      361 caagataagc attaatccct taacagggtc ttgaaaaaca gctgtcactc ccgcaaaggc
      421 gggagtccag aaaacctgcc ttaatacgaa ttaacgagtt actggattcc cgcctctgcg
      481 ggaatgacgt tgttgcatta cgtaacgtgt tttcaacacc ccgctaaacc ttaaactttg
      541 aacctcgaac cttgtaactt gtaacttgaa acttgttcct ggttcactgg tgcgccagga
      601 aatgcgccag cgcctcgatg tcgccgtcgg acacccggta catgatcccg ttcatgctgg
      661 tgtcggggcc ggtgcgcttg ttgtcgcgat acgatttcag cgtcgcggcg aggtagtcct
      721 cccgctggtt cgccagccgc ggcacctgtt tctggccgct atagtcgtcg agatggcagc
      781 tgccgcatcc catcgatctt gcgagcccgg cgccgcgcgc gcgcaatccg gcatcccctc
      841 cgctgctcgc acgcggcggg gcctggcgcg cgtagtaggc ggcgatgtcc atcaagtccg
      901 ggtcggtggc cttgtccagc atacccgcca tctgcgggac ttcgcgcagt ccttcccgta
      961 tcaggataag ctgcaatacg gtgacttctt ccagctgccc ggcgagccag ggcgtcagcg
     1021 gaagcggcgc gcgtcccggt tcaccgtggc acgctgcgca ggctttcgcc ttcgtctcgc
     1081 cgcgcgccgc gtcgcccctg gactgtgcca tcgccgaccc cgcggcgacg gcgaggctca
     1141 gaaaaacaaa ggcgcggcgg atgtctccca tccgccgcgc cgtgccgggg cgtaacatct
     1201 acttctggta ggtcacccgg taaatggcgc ccacctgctc gtcggatacc agcaacgacc
     1261 cgtcaggtaa ttgcaacacg tccaccggcc ggccccagaa cttgttttcc ttcgggtcca
     1321 tgaagccggt catgaacggc gtcactttgg cgcccttgcc gtcagcgccg accctcacgt
     1381 tcgccacgtc gaaaccgaac aggctgctgc ggttccacga gccccggcgc gccacgaaga
     1441 tcgagttctg gtactccttc gggaacatcc cgccggtgta gaagcgcatg ccgagcgcgg
     1501 cggcgtgcgg acccatgagc gcaaccggca gggtcacgcc cttgcacggg tccttcttct
     1561 tgaagtccat gtcgggaatg ccgttcgcgt ggcaatacgg aaagccgaag aactggccga
     1621 tcttggagac gcggttcagc tcgtcctgcg ggccgttctc ggcctgccag tcgcgcccgt
     1681 tgtcggtgaa ccacagctca ccggtcttcg ggtggaaatc gaagccgacc gagttcctca
     1741 ctccgcgcgc gaccacctcc atgttcgacc cgtccgcgac gtagcgtcgg atctggccat
     1801 gctggtcgct cggctcgcag atgttgcacg gcgagccgac ctggatatag agcttgctgt
     1861 cgggaccgaa ggccatgaac ttccagttgt ggtgcacgtc ggggggcagc ttgaactggg
     1921 ccgtgagctc caccggcttg acgttcgggt catcctcgat gccgtcgtag cggaagacgc
     1981 gattgatggc ggcgacgtag agcgagccgg ccaggtaggc gacgccgttc ggctgggtca
     2041 gcttctgggc gaggatgcgg tgcgtgcgct cgccgccctt gtccttgatt tcgtagactc
     2101 ggccgatgat gcgcgtgccg gcccagattg tgcccttgtc gccgcgcgcc atcatgcgca
     2161 cgccgggcat gccatgcgcc cacagctcga ccttgaagcc cttgggcacc ttgatcctgt
     2221 cgagcggaat ctccgacggc ggcgtcaccg tcatctttgc cgggtgtgcc gacaaattcg
     2281 ggttcttcat ccgttcgggg aaatagccgc ccttggactt gcgcgcttcg acctccttgg
     2341 cgctcggcgg cttggtctgc gggcccacca gctgttgtgc cagggcataa gcgcctgcca
     2401 atccgagcgc gcaggacacg aatgccttga gcggggtcga gcgaagacaa ctttgcagaa
     2461 atgaattcat ggtcgttctc ctctttgatg cagggggatt cccgccgcca gcgcgggaca
     2521 cttaatgata cacacgggat ttcaagcgta aagcgggatt tgaagcgtaa agtggcggcg
     2581 gcgccgaccg tcctgctggt aaactccggg agcatgggag gccgaggccg tcatgaattc
     2641 acacggtaat ttcaggttct ggaccggcgc gttcatggcg ctggccatgc tgggggcgcc
     2701 gccggcgggc ctggcccagg gcccttcgac cggatcagga caggcgtatc cgaacaagcc
     2761 ggtgcggctg gtgctgccgt acccgccggg cgggggctcg gacacgatcg cgcggccgct
     2821 ggcgcagaaa atgtcggaag gcctcgggca acaggtcgtg gtggagaacc gcggcggggc
     2881 gaacggaaac atcgcaatgg aatacgtggc gcgttcggcg cccgacggct ataccttcgt
     2941 cttcgggctg aacgcccagt tcgcgatcaa tcccggcctg taccggaaga tcgcttacga
     3001 cccggtccgg gatttcgcgc cgatttcgct gtttggctcg ggcgtctaca tcctggtcgt
     3061 gcatccttcg ctgccggtga agacggtgag agaactcatc gtgctcgcga agtcgcggcc
     3121 cgggcagatc gcctattcct cctcgggcaa cggcagcggc gggcacctcg ccgcggagct
     3181 gttgaacagc atggccggaa tccgaatgct gcacgtcccg tacaaggggg gcgggccggc
     3241 gctggtggac ctgatagcgg ggcaggtgca ggtcctgttc tcgacgcagc tcgcgggatg
     3301 gccgcacgtg caaagcgggc gcgtccgtgc tctggccgtg agcgccgcga agcggccgtc
     3361 gtcgattccg aacctgccta ccctggcgga ggccggcgtg tcgggctacg acgcggtgcc
     3421 ctggtacggc gtactcgcgc cggcggggac cccgcgcgag gtcgtcgcga gactgcacaa
     3481 cgagatcgtc cgcgcgctca accagcccga cttccgcggc ttcctggtca acaacggggt
     3541 cgagccgatc ggcggcccgc ccgaagacct ggctcgcgcg atcaaggccg acctggtgaa
     3601 gtgggcgaag gtgatcaagg aagccggcgt gcgggtggat tgaaaagcag ggatgaggga
     3661 tgagggcgga gggatgagta tccgggcctc cgtgattgcc ggccaacgat caaatggttg
     3721 agacgatcgc tctgcgtttg ccgcttattg ccgggagaag accatcgaac gcaaggattt
     3781 aggcattgca gtcattggcg ccggccgcat cggggggctg cgcgcacgcc tcgcatcgga
     3841 gcatcccggc gtcaagtttc ttgcggtatc cgacagggaa ccggcgcgcg cgcgcgaact
     3901 cgcgcagaaa accggcgcgc agtttcattc gggcgacaac gccgccgtta tttcgcgccg
     3961 cgaggtcaac gccgtcatcg tctcttccag cgagggggcg cacgtcgagc cgatcctaca
     4021 ggcgatcgag ctcggcaaga ccgtgctcgt cgagaagccg atcgcgctca gcctcgcgga
     4081 cggcgaccgg atcattc
//
LOCUS       DBNGSG010000324         6192 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_104967, whole genome shotgun sequence.
ACCESSION   DBNGSG010000324 DBNGSG010000000
VERSION     DBNGSG010000324.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6192)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6192)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6192
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_104967"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="AC158Q_08470"
     CDS             complement(<1..300)
                     /locus_tag="AC158Q_08470"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3339850.1"
                     /translation="MATLLVIEDEAPLRANIVRLLTAEGYRVIAAADGDEGIRRARED
                     RPDLVICDILMPRVDGYGVLATLRSQPETAATPFIFLTASADKEDFARGLQSGAND"
     gene            complement(304..813)
                     /locus_tag="AC158Q_08475"
     CDS             complement(304..813)
                     /locus_tag="AC158Q_08475"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3339851.1"
                     /translation="MLDNVLIIGRAEASMLEFRPAPTDLAAFCEELADEMRLTAGSRH
                     SLDFSYEGARGTVRADEKLLRHVLVNLISNAFKYSPDGGPVAFQVRVGNGQAAFEVAD
                     RGIGIPPEDQPRLFETFHRARNVGNISGTGLGLAIVRKSLDLHGGSIRFDSAPGRGTS
                     FRVIIPLES"
     gene            complement(787..1158)
                     /locus_tag="AC158Q_08480"
                     /pseudo
     CDS             complement(787..1158)
                     /locus_tag="AC158Q_08480"
                     /inference="COORDINATES: protein motif:HMM:NF012722.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            complement(1213..3111)
                     /locus_tag="AC158Q_08485"
     CDS             complement(1213..3111)
                     /locus_tag="AC158Q_08485"
                     /inference="COORDINATES: protein motif:HMM:NF013736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR3339852.1"
                     /translation="MTAVKSRLDADLLAAAFNDARLGVCFVDERGHFMEVNPAFCEMT
                     GYAPAELLGKSWIVAAPPEIAAQAERFLGAVLADSSKVPDQWKVKRKDGRLVDVLVSF
                     RPLAREVGRRCAVITFVDVTGRIQAERSLKESETRFRQIAENVREVLWVTDPAKSQML
                     YVSPAYERVWGRSVEELLRNPLSFVDAIHPEDRGRVVAAFHKQPRGDYDEVYRIVRPD
                     GSVRWIRDRAFPVKDGGGEVYRVTGIAADITDTKLAEAESRRLQTDLERRISERTEAI
                     RRQSAVLLELAALDKSDKRAAFEKILAADARTLGVERVSYWSIVGATAAIECEALFVL
                     TRGATDPAFAGTRLSATDYPTYFSAVLANRPVAANRAQTDAATREFARGYLEPLGITS
                     MLDVPVWFQGRVVGVICHEHIGPAREWSGEDISFASSIASMVSLALEESRWQEAMDAL
                     TRSEEKYRQVVENANEAIAVVQDGRIRYANPQCTRLSGFSLEELYARPFLEFVHGDDA
                     QLVLGNYMKRMRGEQTDSSYEFRIIDKAGLTRWVHINAVAMEWDGRPATLNFLADVTE
                     RHQLQENLARSLAEREVILKTAPVGISFASGRRHRWVNDAFAAMLGYDKNELVGQTSL
                     MHFDDRAS"
     gene            3177..4169
                     /locus_tag="AC158Q_08490"
     CDS             3177..4169
                     /locus_tag="AC158Q_08490"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin--[biotin carboxyl-carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HIR3339853.1"
                     /translation="MKALTANVLKRLAGGGFHSGSALAREFGVSRASVWLAIRDIETL
                     GVEVYKVHGRGYRLPQPLVLLDRAAIERRLGGHAARFVLDIRDAANSTNTLLVERAVA
                     GAESGTVIAAEWQPGGRGRLGRAWHAGVGEALTFSLLWRFQRGAGALSGLPLAVGVAL
                     ARALAAAGAPAAALKWPNDVLWRGRKLCGILIELAGDALGPTAAVIGIGLNVRLSDAT
                     RARIDQPVTDLEAARGAAPDRNELLALVLIELAGVLETFAREGFAPLRAEWQRLHAHQ
                     EKRVTLLLPDGSRATGRARGVAEDGSVLLETRSGVKRYHSGEISLRSASAVKIK"
     gene            4324..5058
                     /locus_tag="AC158Q_08495"
     CDS             4324..5058
                     /locus_tag="AC158Q_08495"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HIR3339854.1"
                     /translation="MRILAIDAGNSRIKWGAHEDDSWRVQGGIATARAARLAREWSGL
                     DAPDAIIAANVAGARVARSIAAAARRFRRRVRFLKGAESQCGVTNSYDRPAQLGADRW
                     AALIGARHLHRGPCVVVNAGTTLTVDALAADGVFLGGMIVAGPDLMRGALARSTAQLR
                     PRRGNFAYFPARTADAIESGAVNALAGAVERMLHFMRQAGQAAPLTVVSGGAAPFLAP
                     HLNGTVEVVDNLVLEGLLRIALEKKA"
     gene            5206..5919
                     /locus_tag="AC158Q_08500"
     CDS             5206..5919
                     /locus_tag="AC158Q_08500"
                     /inference="COORDINATES: protein motif:HMM:NF016895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR3339855.1"
                     /translation="MRALFLLLVLANLMFFAWAHVAREGAGTEDQIRQLQISPEKIRL
                     LKAAGQVVTDKPRIPGKAIPPALPKSALSAPAACLEWGVFAGPGVARAESALAQLELP
                     EGRVGRVVTDAGGYWVHMPPQKTRADADRKLAELKELGVTEFYLVQEPAKWRNAISLG
                     IFRTEEAAQAFLAKLKERGVRTAVAARRENFLKQVAFYVREPGEATVARLTLIQQEFP
                     GSEIKAGPCPPAAAVAAKG"
BASE COUNT          951 a         2064 c         2156 g         1021 t
ORIGIN      
        1 atcgttggcg ccactctgca ggccgcgcgc gaagtcctcc ttgtcggcgc ttgccgtgag
       61 gaagatgaag ggggtcgccg ccgtctccgg ttgcgagcgc agcgtcgcga gcacgccgta
      121 gccgtccacc cgcggcatga ggatatcgca gatcaccagg tccggccggt cctcgcgcgc
      181 gcgccggatg ccctcgtcgc cgtcggcggc cgcgatcacc cggtagcctt ccgcggtgag
      241 caggcgcacg atattcgcgc gcagcggcgc ttcatcctcg atgacgagca gcgtggccac
      301 gcgtcagctt tcaagcggaa tgatgacgcg gaaggacgtg ccgcgtcccg gcgcgctgtc
      361 gaagcggatg gagccgccgt gcaggtcgag cgattttctc acgatcgcca gtcccagccc
      421 ggtgcccgag atgttgccga cgttgcgcgc gcggtggaag gtttcgaaca ggcgcggctg
      481 gtcctcgggc ggaatgccga tgccgcggtc cgcgacctcg aacgccgcct gcccgttgcc
      541 cacccgcacc tggaatgcga ccgggccgcc atccggggag tacttgaagg cgttggaaat
      601 caggttgacc agcacgtggc gcagcagctt ttcgtcggcc ctgaccgtgc cgcgcgcgcc
      661 ctcgtacgag aagtcgagcg agtgccggct gccggccgtg agcctcatct cgtcggccag
      721 ttcctcgcag aacgcggcga ggtcggtcgg cgcgggcctg aactccagca tgctcgcctc
      781 ggcgcgcccg atgatgagca cgttgtcgag catcttggtc atgcgctcca cggccgcgcg
      841 gatgctctgg tggagctcgt gctgttcctc cgccggcagg cgcgcgttgt aatgctcgag
      901 cagctccgcg gaggacagga tggtggcgag cggggtgcgg aactcgtgcg aggtcatggc
      961 gacgaagcgc gacttcagct cgttcagctc cttctccttt tccagcgcgc gcaggatttc
     1021 ctcctcggcg cgcttgcgct cggtgacgtc cacgttgatc cagatcgcgc cgcgcgcgag
     1081 gtcgccgggg tccaccgcgt tgcccgtgac ctcgcaccag aaccgcgacc cgtccttgcg
     1141 cctcatcagc cgctcggtga cgtaagtccg ccccgcggcg agcgcgggat aggcttcggc
     1201 gccgaacgct tcctagcttg cgcggtcgtc gaagtgcatc agcgaggtct gccccaccag
     1261 ctcgttcttg tcgtagccca gcatggcggc aaaggcgtcg ttgacccagc ggtggcggcg
     1321 gcccgaggcg aagctgatgc cgaccggggc ggtcttcagg atcacctcgc gttcggcgag
     1381 cgagcgcgcc aggttttcct gcagctggtg gcgctcggtg acgtcggcga ggaaattgag
     1441 cgtggcgggc cggccatccc attccatcgc gaccgcgttg atgtgcaccc agcgcgtgag
     1501 gccggccttg tcgatgatgc ggaactcgta gctcgagtcg gtttgctccc cgcgcatgcg
     1561 cttcatgtag ttgccgagca cgagctgggc atcgtcgccg tgcacgaact cgaggaacgg
     1621 gcgcgcgtag agttcctcca gcgaaaaacc cgagagccgc gtgcactgcg ggttggcgta
     1681 acggatgcgc ccgtcctgca ccaccgcgat cgcctcgttc gcgttctcca cgacctggcg
     1741 gtatttctcc tcggaacggg tgagcgcgtc catcgcttcc tgccagcgcg actcctcgag
     1801 cgcgagcgag accatgctgg cgatcgagga ggcgaaggag atgtcctcgc cgctccattc
     1861 gcgcgccggc ccgatatgct cgtggcagat gacgccgacc acccggccct ggaaccagac
     1921 cggcacgtcg agcatcgagg tgatgccgag cggctcgagg tagccccgcg cgaactcgcg
     1981 cgtcgccgcg tcggtctgcg cccggttggc ggcgaccggg cggttcgcga gcaccgccga
     2041 aaaataggtc gggtaatccg tggcggagag ccgcgtgccg gcaaaggcgg ggtcggtcgc
     2101 gccccgcgtc aggacgaaaa gcgcctcgca ctcgatcgcc gccgtggcgc cgacgatcga
     2161 ccagtagctc acgcgctcga ccccgagggt gcgcgcgtcg gcggcgagga tcttctcgaa
     2221 ggcggcgcgc ttgtcggact tgtccagcgc cgccagttcg agcaggaccg cgctctggcg
     2281 gcggatcgcc tcggtgcgct cggagatgcg gcgttcgagg tcggtctgca ggcgccgcga
     2341 ttccgcctcc gccagcttgg tatcggtgat gtcggccgcg atgccggtga cgcggtacac
     2401 ctcgcccccg ccgtccttca ccggaaaggc gcggtcgcga atccagcgca cgctcccgtc
     2461 cgggcgcacg atgcggtaga cctcgtcgta gtcgccgcgc ggctgcttgt gaaaggccgc
     2521 gaccacgcgg ccgcggtcct cgggatgaat cgcgtccacg aaggacagcg ggttgcgcag
     2581 gagctcttcc acgctgcggc cccagacgcg ctcgtaggcg gggctcacgt agagcatctg
     2641 cgacttcgcg ggatcggtca cccacagcac ttcgcgcacg ttctcggcaa tctggcgaaa
     2701 gcgcgtctcg ctctccttca ggctgcgctc ggcctggatg cggccggtca cgtccacgaa
     2761 cgtgatcacg gcgcagcgcc ggccgacttc gcgcgcgagc ggccggaagc tcaccagcac
     2821 atcgaccagc ctgccgtcct tgcgcttcac cttccactgg tcgggcacct tcgacgagtc
     2881 ggcgagcacg gcgccgagga agcgctccgc ctgcgccgcg atctccggcg gcgccgcgac
     2941 gatccaggac ttgccgagca attccgcggg cgcgtagccg gtcatctcgc agaacgcggg
     3001 attgacctcc atgaagtgcc cgcgctcgtc gacgaagcac acgccaaggc gtgcgtcgtt
     3061 gaaggcggcg gcgagaaggt ccgcatcgag gcgggatttc acggcggtca tggcgctgct
     3121 cgcggcgagg cgagcggttt tcccccctgc gggatgggct attctatacc cgatccatga
     3181 aggccctgac cgcgaacgtg ctgaagcggc ttgccggagg cggtttccat tccgggtcgg
     3241 cgctcgcgcg cgaattcggc gtctcgcgcg cgagcgtgtg gctcgcgata cgcgatatcg
     3301 agacgctggg cgtcgaggtc tacaaggtgc acggccgcgg ctaccggctg ccgcaacccc
     3361 tcgtgctgct cgatcgcgcc gccatcgagc gccgcctggg cgggcacgcg gcgcgcttcg
     3421 tgctcgacat ccgcgacgcc gcgaattcga ccaataccct gctcgtcgag cgcgcggtgg
     3481 ccggcgcgga gagcgggacc gtgatcgccg ccgaatggca gcccggcggg cgcgggcgcc
     3541 tcggccgtgc ctggcatgcg ggcgtggggg aggcgctcac cttttcgctg ctgtggcggt
     3601 ttcagcgcgg cgccggcgcg ctctccgggc tcccgctcgc ggtcggcgtg gcgctcgccc
     3661 gcgcgctcgc cgcggccggc gcgccggccg cggccctgaa atggcccaac gacgtgctgt
     3721 ggcgcggccg caagctctgc ggcattctca tcgagctcgc gggcgacgcg ctggggccga
     3781 ccgccgcggt gatcggcatc gggctgaatg tgcggctgtc ggacgcgacg cgcgcgcgca
     3841 tcgatcagcc cgtgaccgat ctcgaggcgg cgcgcggcgc cgcgcccgac cgcaacgagc
     3901 tcctcgcgct cgtgctgatc gagctcgccg gcgtcctgga aaccttcgcg cgcgagggtt
     3961 tcgcgccgct gcgcgccgaa tggcagcgcc tgcacgccca ccaggagaag cgcgtgacgc
     4021 tgctgcttcc cgacggctcc cgcgcgaccg ggcgcgcgcg cggcgtggcg gaggacggat
     4081 cggtcctgct cgagacgcgc tcgggcgtga agcgctatca cagcggggaa atcagcctgc
     4141 gctctgcctc cgcagtgaaa ataaaataga caggatcaac agaacttcat cgaaaacccg
     4201 tcaacaccgt caatggacag gattaacagg atttacaaga ttaaggcaca tagagttccc
     4261 aaagttgttg tagtttgaat cctgttaatc ttgttaatcc tgtctattgt ttttgacctt
     4321 gacgtgagaa tactcgccat cgacgccggc aactcccgca tcaagtgggg cgcgcacgag
     4381 gacgactcgt ggcgggtgca gggcgggatc gccacggcgc gggcggcacg gctcgcgcgg
     4441 gaatggagcg ggctcgatgc gccggacgcc atcatcgccg ccaatgtcgc cggtgcgcgc
     4501 gtggcgcgct cgatcgcggc agccgcgcgc cgcttcaggc gccgcgtgcg attcctgaag
     4561 ggtgcggaga gccagtgcgg cgtgacgaat tcgtatgatc gcccggcgca gctcggcgcc
     4621 gaccgctggg ccgcgctgat cggcgcccgg cacctgcacc gcggcccatg cgtggtggtc
     4681 aatgcgggca cgacgttgac cgtggacgcg ctggccgcgg acggcgtttt cctcgggggc
     4741 atgatcgtcg cgggaccgga tctcatgcgc ggcgcgctgg cccgcagcac ggcgcagctg
     4801 cgcccgcggc gcgggaactt tgcctacttc ccggcgcgca cggcggatgc gatcgaaagc
     4861 ggcgcggtca acgcgctcgc gggcgccgtg gaacgcatgc tccacttcat gcggcaggcg
     4921 ggccaggcgg cgccgctgac ggtggtctcg ggcggcgcgg cgccctttct cgcgccgcac
     4981 cttaacggca ccgtcgaggt cgtggataat ctcgtgctcg agggcttgct tcgcattgcc
     5041 ctggagaaaa aagcgtgact ggtgattggt gaatggtgaa tggatgaata cggcagggct
     5101 tgagcgccgt tccgagaacc ccccgccccg cgggcgggga gagccaggac accacagtcc
     5161 attcactatt cactattcac tattcacgcc tgttccagcg aagcgatgag agccctgttt
     5221 ctcctgctgg tgctggccaa cctgatgttc ttcgcctggg cgcacgtcgc gcgcgagggc
     5281 gcgggcaccg aggaccagat ccggcagctg cagatttctc ccgagaaaat caggctgctc
     5341 aaggcggcgg ggcaggtggt caccgacaag ccgaggattc cgggcaaggc catcccgccc
     5401 gcgctgccga agtccgcctt gtcggcgccg gcggcctgcc tcgagtgggg cgtgttcgcc
     5461 gggcccggcg tggccagggc cgagagcgcg ctggcgcagc tcgagctgcc ggaagggcgg
     5521 gtggggcgcg tggtgaccga cgccggcggc tactgggtcc acatgccgcc ccagaagacc
     5581 cgggccgacg ccgaccgcaa gctggccgaa ttgaaggagt tgggcgtgac cgagttctac
     5641 ctggtgcagg agcccgccaa gtggcgcaac gcgatttcgc tcggcatctt ccgcaccgag
     5701 gaggcggcgc aggcgtttct cgcgaagctg aaggagcgcg gcgtgcgcac cgcggtcgcg
     5761 gcgcggcgcg agaacttcct gaagcaggtc gcgttctacg tgcgcgagcc gggcgaggcg
     5821 acggtggcgc gcctgacgct catccagcag gaatttcccg gcagcgagat caaggcgggg
     5881 ccgtgtccgc ccgccgccgc tgtcgcggcg aaaggctaga acctttcacc gccaagacgc
     5941 caagaccgcc aagagaatcc atatatatgt agatatatcc acgcatatgc atatatctac
     6001 atctatagag ttatatatgt cggtatacag attcagaact gctgcaatgc tgtctattac
     6061 aagttaggtt cacgtgcgat tctcagagcg actggcactc cgctttcgaa aactgtgcgt
     6121 cggatgtgac cgctatagcc gtgcgatgaa caaggagcac tactggccgg tctggttgat
     6181 taagcgcact gg
//
LOCUS       DBNGSG010000325         6097 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_444728, whole genome shotgun sequence.
ACCESSION   DBNGSG010000325 DBNGSG010000000
VERSION     DBNGSG010000325.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6097)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6097)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6097
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_444728"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..1543)
                     /locus_tag="AC158Q_08505"
     CDS             complement(194..1543)
                     /locus_tag="AC158Q_08505"
                     /inference="COORDINATES: protein motif:HMM:NF018513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR3339856.1"
                     /translation="MFGTGEMFALLAIMLVMVFAGVHVAVALGAGAVIGIYMMQGDIV
                     VVRTFIAGTAYEALRDYVFAVIPLFMLMGDFLAKCGAARDLFALINRLMRRLPGRLAI
                     ATVLANAVFAFVTGVSIAAAAAFSRIAYPEMKRYGYERKFSLGCIAGSACLGMLIPPS
                     VLMIVWGVLTEQAIGKIFIAGILPGFVLVAIYCAYILGVAWLQPERVGAGKRPVGEVP
                     EPEMTRAEFRVVLISTLMVLLLIAAVLGGIWLGFFTPTEGAGVGAALALLLAIWKGMR
                     AKELFQVVLSVGRTSAPLLLLLFCAQLYSRVLSMTGVTNAAKEFFIASGLGPYEVLAI
                     MVVIWFVLGCLIDSISIILLTVPVFGPVAMALGFDPLAFAILGILAIETGLLTPPFGI
                     LVFTVKAAVPREGTVGEIFRGASPYWIALFAVIVVIAAFPQIATWLPAVGTAIATKG"
     gene            complement(1577..2107)
                     /locus_tag="AC158Q_08510"
     CDS             complement(1577..2107)
                     /locus_tag="AC158Q_08510"
                     /inference="COORDINATES: protein motif:HMM:NF016200.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR3339857.1"
                     /translation="MLIRINDWIARALLVVAASLAFLLCFLVCADVIGRVVFNAPVKG
                     TPEIVSISIVIILWLQAAYAIRSGGMIWVDAVHAHLPNRVQRACEVFAALLGVAFFAL
                     VCWGSVDPALYSWTSDEFEGEGALRVPVWPARWIVFLGSLLATFNYVLIAIERLRALV
                     TGGGPPPAAALEDKIV"
     gene            complement(2204..3271)
                     /locus_tag="AC158Q_08515"
     CDS             complement(2204..3271)
                     /locus_tag="AC158Q_08515"
                     /inference="COORDINATES: protein motif:HMM:NF015445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter
                     substrate-binding protein"
                     /protein_id="HIR3339858.1"
                     /translation="MKRFALASGAAALAFSFWSGAAPAEDIKLRIASGHPPANTYVNL
                     MQNFFVPEVTKRVAARTKHKVEFIEGYGGTMIKVADTLEGVQSGIIDIGGYCFCFEPS
                     NLPLHAFQVMLPFGTMDPVVSVKMTREVYNKVPYLSEVFEKKFNQKLIGLIADNGYNL
                     GTSFDWNNVADLKGRKLAGAGLNLKWLEYAGATPVQSSLPEAYTSMQTGVYNGWIMFP
                     SGYVNFKLYEVGKFYTEVGFGAITWHGLTINRARWAKLPKDVQAIILEVGREYEARTG
                     TVNKENYPKQLEQLKGFGTQVKKVPDSVRVDWANSLKGWPQEKATELDKAGLPGTRVL
                     KLALEEAEKLGHKWPLRYPIK"
     gene            3440..3904
                     /locus_tag="AC158Q_08520"
     CDS             3440..3904
                     /locus_tag="AC158Q_08520"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3339859.1"
                     /translation="MPLSHIEHFLVAADDIEQTAEWYCRVLGMKAGPHPDFGFPVKWL
                     YLGGVDIVHIGPSAKSADANQKKYLGRTSQDTGAGTGALDHIAFRASGLKQMMAHLRS
                     QKIAFSERRASGQALYQLFLYDPNGIKIELNYDAAEAEGLQAQVMASDFARG"
     gene            complement(4019..4420)
                     /locus_tag="AC158Q_08525"
     CDS             complement(4019..4420)
                     /locus_tag="AC158Q_08525"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014906058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR3339860.1"
                     /translation="MAFEQALETHDQDFGKFFLARLFGCEFEYTDDACKVSFDLKDFM
                     LNPQGGIHGGVTAFVLDISMGHLLWKKSGAGTTLEMKVQYARAAKAGRLTATGRFLRQ
                     GRSISYLRSELTDESGELIAFATSTWMSLKK"
     gene            complement(4489..4785)
                     /locus_tag="AC158Q_08530"
     CDS             complement(4489..4785)
                     /locus_tag="AC158Q_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HIR3339861.1"
                     /translation="MAKAYWIAFYRSINNPEAFAAYAKLAPAAIQAHGGRFLARGNPA
                     KVYEAGIAQRVVVIEFDSVEKAIAAHDGPGYQEALRVLGKGAVEREMRIVEGIA"
     gene            4930..5274
                     /locus_tag="AC158Q_08535"
     CDS             4930..5274
                     /locus_tag="AC158Q_08535"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR3339862.1"
                     /translation="MPSTTTPSGLIIDDIVVGNGAAAAAGQKVTVHYTGWLTNGTKFD
                     SSRDRNDPFEFPLGAGRVIKGWDEGVAGMKIGGKRKLTIPPALGYGARGAGGVIPPDA
                     TLVFEVELLGVA"
     gene            complement(5388..>6097)
                     /locus_tag="AC158Q_08540"
     CDS             complement(5388..>6097)
                     /locus_tag="AC158Q_08540"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HIR3339863.1"
                     /translation="YTLIMAAGSWGTISTLFKLPFDLQKDLAPVAFIGTSPYVLVAQA
                     TLPVKTMAELVAHAKANPGKLTIAGSTPGSLQRLAGELLKRTAGIDLLYVPFKGPGAV
                     MPDLLGGRLDLAIDNVLVLAPYIRKGQLRALGVTSSKRSVVYPELPTLAETGMPGFHA
                     VGWFGVFAAARTPQPIVARLNAEIVALLKEPATRDRLLAQGAEPLPGPPDVLRKHLAG
                     EIAKWGKVIREAGIKAE"
BASE COUNT         1111 a         2110 c         1860 g         1016 t
ORIGIN      
        1 ccgaccccga ggaccacgcg caggcgatgc tggtcctcgc ctccgacgcc gccggctaca
       61 tcaacggcgt cacgctcgac gtgaacggcg gacggatcat gatgtaaccc ccaccctgcc
      121 ctccccctta tcaaggggga ggatcaagga gggcaccctc ccccttatca agggggagga
      181 tcaaggaggg ggttcatccc ttggtggcta tcgccgtccc caccgccggc agccaggtcg
      241 cgatctgggg aaacgccgca atcacgacga tgacggcgaa cagcgcgatc cagtagggac
      301 tcgcgccgcg gaagatctcg ccgaccgtgc cctcccgcgg caccgccgcc ttcaccgtga
      361 atacgagtat gccgaagggc ggcgtcagca gcccggtctc gatcgcgagg ataccgagga
      421 ttgcgaatgc cagcggatcg aagccgagcg ccatggcgac cggcccgaac accggcacgg
      481 tcagcaggat gatggaaatc gagtcgatca ggcagcccag cacgaaccag atcacgacca
      541 tgatcgcgag cacctcgtac gggccgaggc cggaagcgat gaagaattcc ttcgctgcgt
      601 tggtgacccc ggtcatcgac agcacgcgcg aatagagctg ggcgcagaac agcagcagga
      661 gcagcggggc ggaagtccgg ccgacggaca gcaccacctg gaacagctcc ttcgcgcgca
      721 tccccttcca gatcgcgagc agcagcgcga gcgcggcgcc cacgccggcg ccctcggtcg
      781 gcgtgaaaaa gccgagccag atgccgccca gcaccgccgc gatcagcagg agcaccatca
      841 gcgtgctgat caggaccacc cggaactcgg cccgggtcat ctcgggctcc ggcacttccc
      901 caaccggccg cttccccgcg cccacgcgct ccggctgcag ccaggccacg ccgaggatgt
      961 aagcgcagta gatcgccacc agcacgaagc ccggcaggat gccggcgatg aagatcttgc
     1021 cgatcgcctg ctcggtcagc acgccccaca cgatcatcag cacggacggc ggtatcagca
     1081 tgccgaggca ggcgctgccg gcgatgcagc cgagcgagaa cttgcgctcg tagccgtagc
     1141 gtttcatctc ggggtaggcg atgcgcgaga acgccgccgc cgccgcgatc gagacgccgg
     1201 tcacgaacgc gaacacggcg ttcgcgagca cggtggcgat cgcgagccgc cccggaagcc
     1261 ggcgcatcag gcggttgatc aatgcaaaaa gatcgcgcgc cgcgccgcac ttggcgagaa
     1321 aatcgcccat cagcatgaac aggggaatca ccgcgaagac gtaatcacgc agcgcctcgt
     1381 acgcggtgcc ggcgatgaag gtgcgcacca cgacgatgtc gccctgcatc atgtagatgc
     1441 cgatgaccgc cccggcgccg agcgccaccg ccacgtgcac gccggcgaac accatcacca
     1501 gcatgatggc gagcagcgcg aacatttctc cggtgccgaa catgggcgcg atcgagaggc
     1561 gggcggtccg gggctgctag acgatcttgt cttcgagcgc ggccgcgggc ggcggaccgc
     1621 ctccggtcac cagcgcgcgc agccgctcga tcgcgatcag gacatagttg aacgtcgcga
     1681 gcaggctgcc gaggaagacg atccagcgcg ccggccacac cggcacgcgc agcgcgccct
     1741 ccccttcgaa ttcgtccgag gtccaggagt agagcgcggg atccacgctg ccccagcaca
     1801 ccagggcgaa gaacgcgacg ccgagcagcg cggcgaacac ttcgcacgcg cgctggacgc
     1861 gattgggcag atgcgcatgc accgcgtcca cccagatcat gccgccgctg cggatggcat
     1921 aggccgcctg cagccagagg atgatgacga tggagatcga gacgatctcg ggagtgccct
     1981 tcaccggcgc gttgaagacg acgcgcccga tgacgtccgc gcagacgagg aagcagagca
     2041 ggaacgcgag gctggccgcg acgaccagca gggcccgcgc gatccagtcg ttgatgcgaa
     2101 tcagcatgcc gacatcagga agcgaagacc gcgcgttgaa cggcggcgtg ccatccgttc
     2161 gaacgcgcgg acgtaaaccg gtgcgaagcg tccggattta cggttacttt atcgggtagc
     2221 gcagcggcca cttgtggccg agcttctcag cctcttccag cgcgagcttc agcacccggg
     2281 tccccggcag gccggccttg tccagctcgg tcgccttttc ctgcggccag cccttcagcg
     2341 agttggccca gtcgacgcgc accgagtccg gcaccttctt cacctgggtg ccgaagccct
     2401 tcagctgctc gagctgcttg gggtagtttt ccttgttgac ggttccggtc ctcgcctcgt
     2461 actcgcgccc gacttcgaga atgatggcct gcacgtcctt cggcagcttc gcccagcgcg
     2521 cgcggttgat ggtgagcccg tgccaggtga tcgcgccgaa gccgacctcg gtgtagaact
     2581 tgccgacctc gtagagcttg aaattgacgt agcccgaggg gaacatgatc cagccgttgt
     2641 agacgcccgt ctgcatcgag gtgtaagcct cgggcagcga ggactgcacc ggcgtcgcgc
     2701 ccgcgtactc gagccacttg agattcagcc ccgccccggc gagcttgcgc cccttcaggt
     2761 ccgcgacatt attccagtcg aatgaggtgc cgaggttgta gccgttgtcg gcgatcagcc
     2821 cgatcagctt ctggttgaat ttcttctcga aaacctccga caggtacggc accttgttgt
     2881 acacctcgcg cgtcatcttc acgctcacta ccgggtccat ggtgccgaac ggcagcatca
     2941 cctggaacgc atgcagcggc aggttcgacg gctcgaagca gaagcaatag ccgccgatgt
     3001 cgatgatccc cgactgcacg ccttccagcg tgtccgcgac cttgatcatc gtgccgccgt
     3061 agccctcgat gaactcgacc ttgtgcttgg tgcgcgcggc cacgcgcttc gtcacttccg
     3121 gcacgaaaaa attctgcatc aggttgacgt aggtattggc cggcggatgg ccggaggcga
     3181 tgcgcagctt gatgtcttcg gccggcgcgg caccggacca gaatgaaaac gccagcgcgg
     3241 ctgcgccgga cgcgagtgcg aaccgtttca tgatcccctc ccgaagtgaa atgaacggat
     3301 gatttgtgcc agcgatgttc cggcttctcc ccgccggtgt caagccgcgc gaaacccgtg
     3361 attcgccccc tttcgcgttc cgcctcgctc ttccggcggt gctagaattt cccccgcgcc
     3421 tgcacgccct ggaggtccca tgccgctgtc gcacatcgag catttcctgg tcgccgccga
     3481 cgacatcgag cagaccgccg agtggtactg ccgggtgctc ggcatgaaag cagggccgca
     3541 tcccgatttc gggttcccgg tgaagtggct ctatctcggc ggcgtcgaca tcgttcacat
     3601 cgggccgagc gcgaagagcg ccgatgccaa ccagaaaaag tacctcggcc gcacctcgca
     3661 ggacaccggc gccggcaccg gcgcgcttga ccacatcgcc ttccgtgcga gcgggttgaa
     3721 gcagatgatg gcgcacctgc gatcgcagaa gatcgccttc agcgagcgcc gcgcaagcgg
     3781 ccaggcgctc taccagctct tcctgtacga ccccaacggc atcaagatcg agctgaacta
     3841 cgacgccgcc gaggccgaag gcttgcaagc gcaggtcatg gcctccgact tcgcgcgcgg
     3901 ctgacgccac gcgcttgaag gcaaaagtcg aaaggtggaa gtgcccatca ggcaggtatt
     3961 tccttctgcc tttcgccttt cgcctttcgc ctttcgcctt tcgcctttag tctccctgtc
     4021 acttcttcag ggacatccag gtcgaagtcg cgaacgcgat cagctctccg ctctcgtccg
     4081 tcaattccga gcgcaggtag gaaatgctgc ggccctggcg caggaagcgc ccggtggcgg
     4141 tcagccggcc cgccttcgcg gccctcgcat actggacttt catctccagc gtcgtgcccg
     4201 cgccgctctt cttccacagc aggtgcccca tcgagatgtc cagcacgaac gcggtcaccc
     4261 cgccgtggat cccgccttgc gggttcagca tgaaatcctt caggtcgaac gacaccttgc
     4321 acgcgtcatc cgtgtattcg aactcgcagc cgaagagccg cgccaggaaa aacttcccga
     4381 agtcctggtc gtgagtttcg agcgcctgct cgaacgccac ccgcgcctca gcctcatcga
     4441 ttgaacgcct taccgtcacc tcatcccccc tcccgcatcc ctcatccctc aagctatgcc
     4501 ttcgacgatg cgcatctcgc gctccaccgc gcccttgccc agcaccctca gcgcctcctg
     4561 gtagccgggg ccgtcgtgcg ccgcaatcgc tttctccacg ctgtcgaact cgatcaccac
     4621 cacgcgctgc gcaatccccg cctcgtagac cttggccgga ttgccgcgcg ccaggaacct
     4681 gccgccgtgc gcctggatgg cggccggggc gagcttggcg taggcggcga atgcttcggg
     4741 gttgttgatg gagcggtaaa aagcgatcca gtaagctttt gccatgagac ctccctgagt
     4801 ccgcgatcga aagagcgtga cgatatcagg tgacatcccg cccgtcactc gtcaccgttg
     4861 ttcgcctttt cccttttccc ttttcccttc agcattcata atgcgccttc tccatcaaaa
     4921 aggaccgcca tgccatccac gaccactccc tccggcctga tcatcgacga catcgtcgtc
     4981 ggcaacggcg cggccgccgc cgccggccag aaagtcaccg tgcactacac cggctggctc
     5041 accaacggca cgaagttcga ctcgagcagg gatcgcaatg accccttcga attcccgctc
     5101 ggcgccgggc gcgtcatcaa gggctgggac gagggcgtcg cgggcatgaa aatcggcggc
     5161 aagcgcaagc tcaccattcc gcccgccctt ggctacggcg cgcgcggcgc cggcggcgtc
     5221 attccgcccg acgccaccct cgtctttgaa gtggaactgc tcggcgtcgc ctgatccagt
     5281 ttcaagttac aagtcacaag tttcaagttt caagtttcaa gtttcaagaa ttggatcact
     5341 tgtaacttgc tacttgtacc ttgtgactca ggcatcaaag cggagcttca ctccgctttg
     5401 atgccggctt cgcgtatgac cttcccccac ttcgcgatct cgcccgcgag gtgcttgcgc
     5461 agcacgtcgg gcgggccggg cagcggttcc gcgccctggg cgagcaggcg atcgcgcgtc
     5521 gccggctcct tcaacagcgc cacgatttcg gcattgagcc gggcgacgat gggctgcggc
     5581 gttctcgcgg ccgcgaaaac gccgaaccag cccaccgcgt ggaaacccgg catgcccgtt
     5641 tccgcgagcg tcggcagctc gggatagacg accgagcgct tcgagctggt cacccccagc
     5701 gcgcgcaact ggcccttgcg gatgtacggc gcgaggacca gcacgttgtc gatggcgaga
     5761 tccagccggc cgcccagcag gtccggcatc accgcgccgg ggcccttgaa cggcacatag
     5821 agcaggtcga tgccggccgt gcgtttcagg agttcgcccg ccagccgttg cagcgagccc
     5881 ggcgtggacc ccgcgatggt cagcttgccc gggttggctt tcgcgtgggc gaccagctcc
     5941 gccatcgttt tcaccggcag cgtcgcctgc gccaccagca cgtacggcga ggtcccgatg
     6001 aacgcgaccg gcgccaggtc cttctgcagg tcgaacggca gcttgaagag cgtgctgatc
     6061 gtgccccagg agcccgccgc catgatcagc gtgtagc
//
LOCUS       DBNGSG010000326         2297 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3501398, whole genome shotgun sequence.
ACCESSION   DBNGSG010000326 DBNGSG010000000
VERSION     DBNGSG010000326.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3501398"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="AC158Q_08545"
     CDS             <1..721
                     /locus_tag="AC158Q_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004632841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HIR3339864.1"
                     /translation="FVAYPEAPWIEISREFVEGLGLEFNPLTTQIEPHDGLAELFHLL
                     IRFNTITLDFVRDVWTYISLGYFRQKVVAGEVGSSTMPHKVNPIDFENSEGNLGVANA
                     LLRHMSEKLPVSRWQRDLTDSTVLRNMGVAFGHALLGYDSCLRGLAKLEADRGRMAEA
                     LDANWEVLAEPIQTVMRRYGIAGGYEKLKELTRGKEGITRDTLHALIRSLAIPDADKK
                     RLLALTPATYIGKATELARRI"
     gene            826..1356
                     /locus_tag="AC158Q_08550"
     CDS             826..1356
                     /locus_tag="AC158Q_08550"
                     /inference="COORDINATES: protein motif:HMM:NF014664.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3339865.1"
                     /translation="MPRISLIDDNATPDIAALSAKIRGARGGQLHDFYRALLHTPGLA
                     SAWFDFNNAVRFQTGIDDRVRELAIMRVAALTGCDYVWKVHQAQYAAPAGVTPREVEA
                     LRDWSRSDVFAAREGALLAYVDAMTRDVAVPDAVFGKLREHFSEREIVDVTVLIAAYN
                     MHTRVLMALGISPDRK"
     gene            1398..>2297
                     /locus_tag="AC158Q_08555"
     CDS             1398..>2297
                     /locus_tag="AC158Q_08555"
                     /inference="COORDINATES: protein motif:HMM:NF012607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3339866.1"
                     /translation="MKKSSKTGTKNEKRKTRNGRRPVVVVEDDPFPRLLQVILDPAAP
                     AARVAAFSHFFAHELPDFTGWCESLRARLKRLYPAEVRLVPDQAGLMAALPGATIAVT
                     ESLAMGRAEIAAAGGALRVIQKYGTVLSGIDTPACKRAGIRVLALRRRANISCAEHAF
                     GLMLALARKIRETAGLVSVEQLQAAGYSPTQYDRAHTASANWARITGTRNLFGRQIGL
                     VGFGEIGREMALRAAAFGMRIAYTRRRRLSTQDESRYGAAYAPLDELLASSDYVSLHL
                     PLSDSTRGIIGRREFGLIKPGAIL"
BASE COUNT          405 a          780 c          729 g          383 t
ORIGIN      
        1 cttcgtggcc tacccggagg cgccctggat cgagatctcc cgcgagttcg tcgaaggatt
       61 gggactggag ttcaacccgc taaccacgca gatcgagccg cacgatggcc tcgcggagct
      121 gttccacctg ctgatccgct tcaatacgat cacactcgat ttcgttcgag acgtctggac
      181 gtatatctcg ctgggctact tccggcagaa ggtcgttgcc ggcgaagtcg gctcctcgac
      241 gatgccgcac aaggtgaacc cgatcgactt cgagaattcc gaaggcaacc tgggcgtcgc
      301 caacgcgctg ctgcggcaca tgagcgagaa gctgccggtg tcgcgctggc agcgcgatct
      361 caccgattcc accgtgctgc gcaacatggg tgtcgcgttt ggccacgccc tgctcggcta
      421 cgattcctgc ctgcggggcc tcgccaagct cgaagccgac cgcggccgca tggccgaagc
      481 gctcgatgcc aactgggaag tgcttgccga gccgatccag accgtgatgc ggcgctacgg
      541 gatcgcgggc ggctacgaga agctgaagga gttgacgcgc ggcaaggaag gcatcacgcg
      601 cgacacgctg cacgccctga tcagaagcct cgccatcccg gacgcggaca agaaacgact
      661 gctcgcgctg actcctgcca cctatatcgg caaggccacc gagcttgccc ggcgcatctg
      721 attcagaaag gcgtgatatg ggataaggga taaggcttcc ggcatcgccg gagctgtaag
      781 acccgtctcc cctatcccct gtcacttatc acgcatcaca agacaatgcc gcgcatttcc
      841 ctcatcgacg acaacgccac gcccgacatc gccgcgctct ccgcgaaaat ccgcggcgcc
      901 cggggcggcc agttgcacga tttctaccgc gcgctgctgc acacgcccgg cctcgcgtcc
      961 gcctggttcg atttcaacaa cgcggtgcgg ttccagaccg gtatcgacga ccgggtgcgc
     1021 gagctcgcca tcatgcgggt cgcggcgctg accggttgcg attacgtctg gaaagtacac
     1081 caggcgcagt acgcggcccc ggccggcgtg acgccgcggg aagtcgaagc cctgcgggat
     1141 tggagccggt cggacgtctt cgccgcgcgc gagggcgcgc tgctcgcgta cgtggacgcc
     1201 atgacccggg atgtcgccgt cccggacgcc gtattcggaa aattgcggga gcatttcagc
     1261 gagcgcgaga tcgtggacgt aacggtgctg atcgccgcct acaacatgca cacccgggtg
     1321 ctgatggcgc tgggcatcag ccccgacagg aagtgactcg ttcctcgttt ctcgttgctc
     1381 gttcgatgaa aaacgtcatg aagaaaagca gcaagacagg aacgaagaac gagaaacgaa
     1441 aaacgagaaa cggacgacga ccggtggtcg tggtcgagga tgaccccttc ccgcggcttc
     1501 tccaggtgat cctggaccct gccgcccccg cggcccgcgt cgcggctttc tcgcactttt
     1561 tcgcccacga gctgcccgat ttcaccggct ggtgcgagag cctgcgtgcg cgcctgaagc
     1621 gtctctatcc tgccgaggtg cggctcgtcc ccgaccaggc gggcctgatg gccgccctgc
     1681 ccggcgcaac catagcggtc acggagtcgc tcgccatggg cagggccgag atcgccgccg
     1741 caggcggcgc gctgcgcgtc attcagaaat acggcaccgt gctctccggc atcgatacgc
     1801 ccgcctgcaa gcgcgccggc atccgggtgc tcgcgctgcg gcggcgcgcc aatatctcgt
     1861 gcgccgagca cgcgttcggc ctgatgctgg cgctcgcccg caagatccgc gagaccgccg
     1921 ggcttgtcag cgtcgagcag ttgcaggcgg ccggctactc gcccacgcag tacgaccgcg
     1981 cgcacacggc aagcgccaac tgggcgcgca tcaccggcac gcgcaatctg ttcggaaggc
     2041 aaatcggtct cgtcggattc ggcgaaatcg ggcgcgagat ggcgctgcgc gccgcggcgt
     2101 tcggcatgcg catcgcctat acccggcgcc gccggctttc tacccaggac gagtcgcgct
     2161 atggcgcggc ctacgcgccg ctcgacgagc tgctggcttc cagcgattac gtgagcctgc
     2221 acctgccgtt gagcgactcc acgcgtggca tcatcggccg gcgcgagttc ggtttgatca
     2281 agcccggcgc gatcctc
//
LOCUS       DBNGSG010000327         5123 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4519947, whole genome shotgun sequence.
ACCESSION   DBNGSG010000327 DBNGSG010000000
VERSION     DBNGSG010000327.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5123)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5123
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4519947"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="AC158Q_08560"
     CDS             <1..703
                     /locus_tag="AC158Q_08560"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF021535.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIR3339867.1"
                     /translation="TYFGQTEAVGEQGTPVTGAMMAGSLVRFIYLVFHEDCHDQFDFP
                     QGIEEALCNVIAYNAIVAYAGDRTQSNALERVAMRRYAQRESARTRQAKVFYEQLEGL
                     YIRHYKKELSVQALLEERTRLFGRAERALAWDKGSLNNVGIANDMTYSRHFPYLEQVF
                     DALGRDLARTVEFFRKIDAAKPARTRIVKQRRLKDDRNVEFVRAYEAAVLELIRKELP
                     AASGDAAKMGDAVKK"
     gene            complement(793..2040)
                     /locus_tag="AC158Q_08565"
     CDS             complement(793..2040)
                     /locus_tag="AC158Q_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3339868.1"
                     /translation="MNTAATTTGAAPRSFKDVWLISAGHGLTHWYTATFFLLLPLIGK
                     ELGLSYTEIGLIITVQHAVGAISNLPGGMVVDMIGKMGYLMAASLFWVGFPYALMSLT
                     DSYWMLLVCVTLVGIGNNIWHPAAISALAHRYPDRKGLVLSFHGMGGNAADAIAPLVV
                     GALLAWFTWRTVVVINVVPGIVMATMILVMLGAFSASASRGESINAGGRKRSLADYVK
                     EFGSLLRNKTLMLVCVSSAFRTMTQAGLLTFLPVYLAYEMGYSPFLVGLCLTLLQAGG
                     FVASPLAGHLSDRMGRKRIVISSALLTGVMIVAMALAGKSLWFILFVALVGFFLYAMR
                     PVLQAWAVESTPPHLAGSGVGLQFGITAVGASISPALFGIIADAWDIHTAFFFLAGTI
                     IASNVLVFFMPADAARKAKPAAA"
     gene            2242..4389
                     /locus_tag="AC158Q_08570"
     CDS             2242..4389
                     /locus_tag="AC158Q_08570"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043758 - acetate-CoA ligase (ADP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HIR3339869.1"
                     /translation="MRTDLERFFNPASIAIIGASQDFMTISGQPLKHLQSHHYQGRLY
                     PVNPRYPEVGGVKCYPALAEVPETPDLVLILVNAARVADMLRQCGAKGVPYVIIFSSG
                     FSEMGGEGVKLQRELAEIALQFRIGVIGPNCQGMMNVADGVFAGFGSVFFTDYEPGRV
                     SMVSQSGGFGFSVMNLSSKDGGLPFRQMVTTGNEIGVSTLDFIDYFIRDSRTDIIAGY
                     LEGAKDAHRLPEIGQKALAAGKPILMWKVGNTEQGQKAAASHTANLGGAMALYKAAFR
                     QNGIIQVEDIQDVVDYGRAFRSGHLPKGNRLAIITISGGAGILMTDECIGRGMRLADL
                     APETARKLKEFVPSFGSISNPVDVTAAIFNDLTLINRTLQAIVDDPGVDCIAMINASL
                     QGEIASKIAGEIVAVAGRTDKPICIAWSARDVMAPEAYATLEAARIPHYKSPVRCGRA
                     MAAVWWYAEAKRRNETQRAEKPLVLSKPAAAKMLAGKTGDVSEHTAKRVLAEYGIPVT
                     QEELATSKEQVLAAAKRIGYPVAIKVQSSDIPHKTEAKAVKLNLGSDEELASAYEEVL
                     ANARAYNKNATIEGVLVQEMIKDGVEAILGVTNDALFGPAVMFGLGGIFAEVLKDVSF
                     RLAPVTPSVAREMVEEIAGYPVLAGARGRPRADVAALTDAIVRLSALAVDLKDSVSEL
                     DINPLFVFAEGKGVKAADALIRVKTAESKNRQD"
     gene            4530..>5123
                     /locus_tag="AC158Q_08575"
     CDS             4530..>5123
                     /locus_tag="AC158Q_08575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3339870.1"
                     /translation="MNLTPEVRQLCQEVRRFIQKEVDPRSRWIEENDAIPDDLMKMAR
                     ELGLFGLTIPEQYGGIALDLAGKCAIEEEMGRTNYGFATVIGNHTGIGTTGIVALGSE
                     PQKRKYLPRMASGEWVGCFSLTEPHAGSNPADIRMSAVKKGDRYILNGEKIYCTNGGI
                     AQVFTVMAVTDRAKGIKGISAFIIERGFPGFSVGKNEL"
BASE COUNT         1031 a         1555 c         1691 g          846 t
ORIGIN      
        1 cacttacttc ggccagacgg aagcggtggg cgagcagggc acgccggtca ccggcgcgat
       61 gatggccgga tcgctggtgc ggttcatata cctggtgttc cacgaggatt gccacgatca
      121 attcgacttc ccgcaaggca tcgaggaggc gctctgcaac gtcattgcct acaacgccat
      181 cgtcgcgtat gccggcgaca ggacccagtc gaacgccctc gagcgcgtgg cgatgaggcg
      241 ctatgcgcag agggaatccg cgcgcacccg ccaggcgaag gttttctacg agcagctcga
      301 ggggctctac atccgccatt acaaaaagga gctgtcggtg caggcgctgc tggaggagcg
      361 cacgcggctg ttcggaaggg cggaaagagc gctggcctgg gacaagggct ccctcaacaa
      421 cgtgggcatc gccaacgaca tgacgtatag ccggcacttt ccgtacctgg agcaggtgtt
      481 cgacgcgctc ggccgcgacc tcgcgcggac cgtcgaattt ttcaggaaaa tcgacgcggc
      541 caagccggcc cgcacccgta tcgtgaagca gcgccgcctc aaggacgacc ggaacgtgga
      601 attcgtccgc gcctacgagg cggccgtgct ggagctgatc aggaaggaat tgccggcggc
      661 atcgggtgat gcagcgaaga tgggtgacgc agtgaagaag tgacgcagtg acggttttcg
      721 cgaatcgtag aacgtaagtc gggaacgcgc ttcaggtcct acggttcacg actcacgttt
      781 accgtgtcac ggtcaggccg cggcaggctt cgccttcctg gcggcgtcgg cgggcatgaa
      841 gaagacgaga acgttggagg cgatgatggt gccggcgagg aagaagaacg ccgtgtggat
      901 gtcccaggca tcggcgatga tgccgaacag cgccggcgag atgctggcgc ccaccgcggt
      961 gatcccgaat tgcagaccca ctccgctgcc ggcgagatgc ggcggtgtgg actcgacggc
     1021 ccaggcctgg agcacgggac gcatggcgta gaggaagaag ccgaccagcg cgacgaacag
     1081 gatgaaccag aggctcttgc cggcgagcgc catggccacg atcatcacgc ccgtcaacag
     1141 cgcgctggag atgacgatgc gcttgcgtcc catcctgtcc gacaggtgcc cggcaagcgg
     1201 gctcgccacg aagcctcccg cctgcagcag cgtcaggcac aggcccacca gaaacggcga
     1261 ataacccatt tcgtaggcaa ggtagaccgg caggaaggtg agcagccccg cctgcgtcat
     1321 ggtgcgaaac gccgagctca cgcacaccag catgagcgtc ttgttcctga gcaggctgcc
     1381 gaattccttc acgtagtccg ccaggctgcg ttttctgccg ccggcgttga tcgattctcc
     1441 ccgggacgcg gacgccgaaa atgcgcccag catgacgagg atcatggtgg ccatgacgat
     1501 gccgggcacg acgttgatca ccaccacggt gcgccaggtg aaccaggcga gcagcgcgcc
     1561 caccaccagc ggcgcaatcg cgtcggcggc gttgccgccc atgccgtgga acgaaagcac
     1621 cagccccttg cggtcgggat agcggtgggc gagcgccgag atcgccgccg ggtgccagat
     1681 attgttgccg atgccgacca gggtcacgca gaccagcagc atccagtagc tgtcggtgag
     1741 gctcatcagc gcgtacggga agccgaccca gaacagcgag gccgccatga ggtagcccat
     1801 cttgccgatc atgtccacga ccatgccgcc cggcaggttg gagatcgcgc ccacggcgtg
     1861 ctgcacggtg atgatcagcc cgatctcggt ataagagagg ccgagctcct tgccgatcag
     1921 cggcagcagc aggaaaaagg tcgcggtgta ccagtgcgtg agaccatggc cggcggagat
     1981 cagccacacg tccttgaacg agcgcggcgc ggctccggtc gtagtggcgg cggtgttcac
     2041 gatgtggtcg gaaggttctg cgagtacgcg cgaatgatag cacgggcgcg acgctgctca
     2101 cggcgcgagc atatataatt tccgttaaat ccaggcgctt cgcgctggtt taacttcgcg
     2161 cgttcgaacc gacgaatgga tcgcggttta acgcgcgata tccacgataa gaactctccg
     2221 gcagcccggg cacttcagga aatgcgtacc gacctcgaac gcttcttcaa ccccgcctcg
     2281 atcgcgatta tcggcgcctc gcaggacttc atgaccatca gcggccagcc gctgaagcac
     2341 ctgcagtcgc atcactacca gggcaggctc tacccggtca acccgcgcta cccggaagtg
     2401 ggcggcgtga agtgctatcc cgcgctcgcc gaggtgcccg agacgcccga cctggtgctg
     2461 attctcgtca acgctgcgcg cgtggcggac atgctcaggc aatgcggcgc gaagggcgtg
     2521 ccgtacgtga tcatcttcag ctcgggcttc tccgagatgg gcggcgaggg cgtgaagctg
     2581 cagcgcgaac tcgcggagat cgcgctgcag ttccggatcg gcgtgatcgg ccccaactgc
     2641 cagggcatga tgaacgtcgc cgacggcgtg ttcgccggct tcggctcggt gttcttcacc
     2701 gattacgagc cggggcgcgt cagcatggtt tcgcagtcgg gcggtttcgg cttctcggtg
     2761 atgaacctct cgtccaagga cggcgggctg ccgtttcgcc agatggtcac caccggcaac
     2821 gaaatcgggg tctccacact cgacttcatc gactacttca tacgggattc caggaccgac
     2881 atcattgccg gttacctcga aggcgcgaaa gacgcgcatc gcctgccgga gatcgggcag
     2941 aaagcgctcg ccgccggcaa gccgatcctg atgtggaaag tcggcaatac cgagcagggg
     3001 cagaaagcgg cggcttcgca caccgccaac ctcggcggcg cgatggcgct gtacaaggcc
     3061 gcgtttcgcc agaacggcat catccaggtc gaggatatcc aggacgtagt ggactacggt
     3121 cgcgccttcc gcagcgggca cctgccgaag ggcaaccgtc tcgccatcat caccatctcc
     3181 ggcggagcgg gcatcctcat gaccgacgag tgcatcggcc gcgggatgcg cctcgcggac
     3241 cttgcgccgg agacggcgag gaagctgaag gagttcgtgc cgagcttcgg ctcgatcagc
     3301 aacccggtgg acgtcactgc ggcgattttc aacgatctca ccctcatcaa ccgcacgctg
     3361 caggcgatcg tggacgaccc cggcgtggac tgcattgcca tgatcaacgc ctcgctgcag
     3421 ggcgagatcg cgagcaagat cgccggcgag atcgtcgcgg tcgcgggcag gaccgacaag
     3481 ccgatctgca tcgcgtggag cgcgcgtgac gtcatggcgc cggaggccta cgcgacgctc
     3541 gaggccgcgc gcatcccgca ctacaagtcg ccggtgcgct gcgggcgggc gatggcggcg
     3601 gtgtggtggt atgcggaggc aaagcgccgc aacgagacgc aacgcgcgga gaagccgctg
     3661 gtgctcagca agccggctgc ggccaaaatg cttgccggca agaccggcga cgtgtccgag
     3721 cacaccgcca agcgcgtgct ggccgagtac ggcataccgg tgacgcagga ggaactcgcg
     3781 acctcgaagg agcaggtgct ggccgccgcg aagcgcatag gctacccggt tgcgatcaag
     3841 gtgcagtcat ccgatatccc gcacaagacc gaagcgaagg cggtaaaact gaatctcggc
     3901 tccgatgaag aactggcctc ggcgtacgag gaagtgcttg cgaacgcgcg tgcttacaat
     3961 aaaaacgcaa caattgaagg tgtcctggtc caggaaatga tcaaggatgg agtggaagcc
     4021 attctgggcg tcaccaacga tgccctgttc ggcccggcgg tgatgttcgg gctgggtggc
     4081 atcttcgcgg aagtgctgaa ggacgtgtcg ttccggctcg cgccggtgac cccgtccgtc
     4141 gcgcgcgaga tggtggagga gatcgccggc tacccggtgc tggcgggagc gcgcgggcgc
     4201 ccgcgcgccg acgtggctgc gctcacggac gcgatcgtgc gcctgtccgc gctcgcggta
     4261 gacttgaagg actccgtttc cgaactcgac atcaatccct tgttcgtttt tgccgagggt
     4321 aagggtgtga aggcggcgga tgcgctgatc cgtgtcaaaa ccgctgaatc aaaaaataga
     4381 caggattaac aggattaata agattaacaa gatttcaaac atgaacagag gtatcgtttc
     4441 gttccggatc taatcctgta aatcttgtta atcctgtcta ttaatggggt agacgggttt
     4501 atgagttatt ttgtaaatcc tgtccattta tgaacctgac acctgaagtc cggcaactct
     4561 gccaggaagt gcggcgcttc atccagaagg aagtcgaccc gcgctcgcgc tggatcgagg
     4621 agaacgacgc gatccccgac gatctcatga agatggcgcg cgagctgggg ttgttcgggc
     4681 tcacgatccc ggagcagtac ggcggcatcg cgctcgacct cgcgggcaag tgcgcgatcg
     4741 aggaggagat ggggcgtacc aattacggct tcgccaccgt catcggcaac cacaccggca
     4801 tcggcactac cggcatcgtc gcgctcggtt cggagccgca gaagcgcaaa tacttgccgc
     4861 gcatggcgag cggcgagtgg gtgggctgct tctcgctcac ggaaccgcac gccggctcca
     4921 accccgccga catccggatg agcgcggtga agaagggcga ccgctacatc ctgaacggcg
     4981 agaagatcta ttgcaccaac ggcggcatcg cgcaggtgtt cacggtgatg gcggtcaccg
     5041 acagggcgaa gggcatcaag ggcatctcgg ccttcatcat cgagcgcggc tttcccggct
     5101 tcagcgtggg caagaacgag ctc
//
LOCUS       DBNGSG010000328         1640 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4691222, whole genome shotgun sequence.
ACCESSION   DBNGSG010000328 DBNGSG010000000
VERSION     DBNGSG010000328.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4691222"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(225..662)
                     /locus_tag="AC158Q_08580"
     CDS             complement(225..662)
                     /locus_tag="AC158Q_08580"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014664.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3339871.1"
                     /translation="MKKTKSRRGYNDRFERGMKVLKKMGRENLMLNQKALSPEMYEMS
                     VGHLFGDVWGRPGLSLRDRQLVTLAANIAMARPTGNHSHYLSSMRLGITRREIIEIMI
                     QVGHYTGWPTLSNAVRQFTEILEEQKAAEKAKKLGRPFKSRLL"
     gene            808..>1640
                     /locus_tag="AC158Q_08585"
     CDS             808..>1640
                     /locus_tag="AC158Q_08585"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3339872.1"
                     /translation="MRRFVHATLALWIAATPALAQQAKPAAGAGDYPNKSIRLVVPSA
                     PGGGTDIIARWIGQGLTDAWGQTVVVDNRGGGGGTVGVAIVAKAPQDGYTMLLGSVGH
                     LTFAPVARKGLPYDARKDIAPITLAANQPFLVAAAPSLQAKSIAELVALAKSRPGEIK
                     YGSGGVGSASHLGIALMELQTGISMLHVSYKGSNPALTAVMGGEVQLVIAGLATVMPH
                     MKSGRVRGLAVTGSKRAQVAPDVPTAAESGAKGYAFDVWYGLVFTGGTPRAVVQKANS
                     EL"
BASE COUNT          267 a          500 c          571 g          302 t
ORIGIN      
        1 cgctgggaga ccatcgagtc ccatacggtc aacttccggc agtcgccgaa attccaggag
       61 ttccgcgggc tggtgggggg attcttcgcg cagccgccgg tcgccgagca tttcgagcgg
      121 gtcacggcga gtcatatctg actcaaggca caagtcacaa gtcacaagtc acaagggcaa
      181 ggcggcgtgt ttccggcggt tttccttttg ccttttgcct tcggctacag caggcgcgac
      241 ttgaacggcc tgcccagttt cttcgccttt tccgcggctt tctgctcctc gaggatctcg
      301 gtgaactggc gcacggcgtt ggagagggtc ggccagccgg tgtagtgccc gacctggatc
      361 atgatctcga tgatctcgcg ccgggtgatg ccgaggcgca tcgagctcag gtaatgcgaa
      421 tggttgccgg tggggcgcgc catcgcgatg ttcgcggcca gcgtgacgag ctgccggtcg
      481 cgcagggaga ggccggggcg gccccagacg tcgccgaaca gatgcccgac cgacatctcg
      541 tacatctcgg gcgagagtgc tttctggttc agcatgagat tttcgcggcc catttttttc
      601 aacaccttca tgccgcgctc gaagcggtcg ttgtagccgc gccgggactt tgttttcttc
      661 atggattggg tctcacagtg tggaatggga tcgatcggaa agcggattat tttttcaacg
      721 ccgcttttgt gtgatgatgt caccctgaag gacgcggagc aagcctgcga tggctcgtcg
      781 gccgcgcccg ctacgaggag aaaagacatg agacggttcg tgcacgcgac gttggcactg
      841 tggattgcgg cgactcccgc cctggcgcag caggcgaagc cggctgcggg tgccggcgat
      901 tacccgaaca agtcgatacg cttggtggtc ccgagcgcgc ccggcggcgg caccgacatc
      961 attgcgcgct ggatcggaca ggggctcacc gatgcctggg gccagacggt cgtggtggac
     1021 aaccgcgggg gcggcggcgg cacggtgggg gtcgccatag tcgccaaggc gccccaggac
     1081 ggctacacca tgctgctcgg cagcgtgggg cacctgacct ttgcgccggt cgcgcgcaag
     1141 gggctgcctt acgatgcccg gaaggacatc gcgccgatca cgctcgcggc gaaccagccc
     1201 ttcctcgttg ccgcggcgcc gtcgctgcag gcgaaatcga tcgcggagct ggtcgcgctc
     1261 gccaagagcc ggccgggcga aatcaagtac ggctcgggcg gcgtcggctc cgcgtctcat
     1321 ctcggtatcg cgctgatgga gttgcagacc ggcatctcga tgctgcatgt gtcctacaag
     1381 ggcagcaacc cggcgctgac cgcggtgatg ggcggcgagg tccagctggt gatcgcaggc
     1441 ctggccaccg tgatgccgca catgaagtcc gggcgggtca ggggcctggc agtgaccgga
     1501 tcgaagcgcg cacaggtcgc gcccgatgtg ccgaccgcgg cggagtccgg ggccaagggc
     1561 tacgcgtttg acgtctggta cggcctcgtg ttcacgggcg gcaccccgcg cgcggtcgtc
     1621 cagaaggcca acagcgaact
//
LOCUS       DBNGSG010000329         4930 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2483179, whole genome shotgun sequence.
ACCESSION   DBNGSG010000329 DBNGSG010000000
VERSION     DBNGSG010000329.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4930)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2483179"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="AC158Q_08590"
     CDS             complement(<1..411)
                     /locus_tag="AC158Q_08590"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3339873.1"
                     /translation="MIKAVLFDLDGTLADTAPDLGYAVNRMRETRGLPPLPSSATRPV
                     TSLGARGLLNAAFGIGPGQPDYGAMREEFLMLYEQNICRETRLFSGIAGLLDTLEARA
                     LRWGIVTNKSERLARMLLGALRLTSRAACIIGGDS"
     gene            complement(496..1191)
                     /gene="ubiG"
                     /locus_tag="AC158Q_08595"
     CDS             complement(496..1191)
                     /gene="ubiG"
                     /locus_tag="AC158Q_08595"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HIR3339874.1"
                     /translation="MINVDPLELEKFSQLAHRWWDPGAEFKPLHDINPLRLGYIDDIA
                     SLRGMRVLDVGCGGGILAESMAAKGAQVTGIDLAEKPLAVAQLHLHESGLPVDYRLIS
                     PEALAHEAPAGFDVVTCMELVEHVPDPAGMVQACADLVKPGGHVFFSTINRNLKSYLL
                     AVVGAEYVLKLLPRGTHDYAKFIKPSELAAMCRASGLGVTGLTGMTYNPFTKIYALGP
                     DTDVNYILHARKA"
     gene            complement(1249..2541)
                     /locus_tag="AC158Q_08600"
     CDS             complement(1249..2541)
                     /locus_tag="AC158Q_08600"
                     /inference="COORDINATES: protein motif:HMM:NF006549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HIR3339875.1"
                     /translation="MEAGWIIPVAPPEVVLTEHAVVVDAGLIRELLPAQLARSRYPGA
                     RRIELPGHALIPGLINLHTHAAMTLMRGLADDRALMDWLHNHIWPVETRLVSEAFVRD
                     GTLLACAEMLRGGVTCFNDMYFHPEAAAQAALAAGMRAALGIIVIEMPSSYASDAQDY
                     LSKGLAMRDSLKYEPLLSFCVAPHAPYSVSDKTFERIAMLQGELDLPLHIHAHETRDE
                     IEQSLARHNTRPLARLQRLGLVDANLIAVHAVHLTEQEGALMAEKGCHIAHCPSSNLK
                     LASGLNPPSRFLKDGVNVGLGTDGAASNNRLDLFTEMRLAALLAKGATGDPTFLPAHT
                     VLEMATLRAARALAVDDRVGTLAPGKCADMAAVDLSAPELAPCYDPLSHLVYAAGREH
                     VSHVWVNGELLVEDGRLTRLDTQEIVAKASYWKEKIKH"
     gene            complement(2632..3393)
                     /locus_tag="AC158Q_08605"
     CDS             complement(2632..3393)
                     /locus_tag="AC158Q_08605"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3339876.1"
                     /translation="MNSAIRITLVGLAAAGMFLPMPASAQFKNQGYLTDSAGNVVRAP
                     GAGVCVRTSDWTPARAIAECDGDLVKKAPTAAPKPGAKPKPDAKPKPEKKANPKMLNI
                     EQKIELQGMPFDKAEMTDDNKAELSKFLAGLRKATKARGPVTFGAVVVTGHTDRLGGL
                     QYNMKLSERRAVVVKDALVASGVDQKLIFWEGKAYKQPIPVTKFCDNKMKRKQLIECL
                     APNRRVTVEVVGRAQSLAKPKPKAKAKPKAKPARR"
     gene            3905..>4930
                     /locus_tag="AC158Q_08610"
     CDS             3905..>4930
                     /locus_tag="AC158Q_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR3339877.1"
                     /translation="MDQIAKETVPVSLEEEMRRSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRILFAMHEANNVWNRPYVKCARVVGDVLGKYHPHGDTATYEALVRMAQDFSMRYTL
                     IDGQGNFGSIDGDSAAAYRYTECRLDRITSDMLADIDMETVDFVPNYDGKEQEPATLP
                     TRVPNLLVNGSSGIAVGMATNIPPHNLSEVVDACQALLKDPDLTIDDLIKIVPAPDFP
                     TRGIIYGVQGVKEGYRTGRGRIVMRARTHFEETEKGGRTYIVVDELPYQVNKASLLMK
                     IGELVREKKIDGISDIRDESDKSGMRAVIELKRGENPEVILNNLWKETQLEDTFGMNM
                     VALVDGQP"
BASE COUNT          907 a         1523 c         1566 g          934 t
ORIGIN      
        1 gctgtccccg ccgatgatgc aggccgcgcg cgacgtcagg cgcagcgcgc ccagcagcat
       61 gcgtgcaagg cgttccgatt tgttggtcac gataccccag cgcagcgcgc gcgcttcgag
      121 cgtatccagc agcccggcga tgccggaaaa cagccgtgtc tcgcggcaga tgttctgttc
      181 gtagagcatc aggaattcct ccctcatcgc gccgtagtcc ggttggccgg ggccgatgcc
      241 gaatgccgcg ttgagcagcc cgcgtgcgcc cagcgatgta acagggcggg tggcggaaga
      301 aggcagcggc ggaagtccgc gcgtttcgcg catgcggtta accgcgtaac ccaggtcggg
      361 cgcggtatcc gccagcgttc cgtcgaggtc gaaaaggacc gccttgatca tgagtgcggg
      421 tctgggttca ggacatcacg cgcagcgcgg tgtcagcctg ttcctatccc cctatccctt
      481 gtcccgtatc acctgtcacg ccttcctggc atgaaggatg taattcacgt cggtatcggg
      541 acccagcgcg tagattttag taaacgggtt gtaggtcatg ccggtcaagc ccgtcacccc
      601 gaggcctgac gcgcggcaca tagcggctaa ctcggaaggc ttgatgaatt tggcgtaatc
      661 gtgagtgccc cgcggaagga gtttcagcac gtactcggcg ccgacgacgg cgagcaggta
      721 cgacttcagg ttgcggttga ttgtggaaaa aaacacatgt ccgcccggtt tgacgaggtc
      781 ggcgcaggct tgaaccatcc ccgccgggtc cggcacgtgc tcgaccagtt ccatgcacgt
      841 gaccacatcg aaacccgcgg gcgcttcgtg cgcgagagcc tcgggcgaga tcaggcggta
      901 atcaaccggc aatcccgact cgtggagatg caactgcgcc accgccagtg gcttctccgc
      961 aagatcgatg ccggtgacct gcgccccttt ggccgccatg ctttcggcca gtatgccgcc
     1021 cccgcatccc acatcgagca cgcgcatgcc gcgcaggctg gcgatatcgt cgatgtagcc
     1081 cagccgcagt ggattgatat cgtgcagcgg cttgaattca gcgccggggt cccaccagcg
     1141 gtgagcgagt tggctgaatt tctcgagttc gagcggatcg acgttgatca ttccgaggac
     1201 aagggaagag tgacgaggag tgattctact agcgccggtt cccgatccct agtgcttgat
     1261 cttctctttc cagtaagacg ccttggcgac gatctcctgc gtatcgagcc gcgtcaaccg
     1321 gccgtcttcc accagcaatt ctccattgac ccagacatgg ctgacatgtt cccgcccggc
     1381 cgcatagacc aggtgcgaca gcgggtcgta gcagggagcc agttccggcg cgctcaggtc
     1441 cacggccgcc atgtcggcac attttccggg tgcgagcgtg ccgacgcgat cgtcgacggc
     1501 gagggcgcgc gccgcgcgca acgtggccat ctcgagcacc gtgtgcgcgg gcaggaacgt
     1561 cggatcccct gttgcgccct tggcaagcag ggcggcaagc cgcatttcgg tgaagaggtc
     1621 cagacgatta ttgctcgctg ccccgtcggt gcccagaccc acgttaacgc cgtccttgag
     1681 gaagcgggac ggcggattga ggccgctggc gagcttcagg ttcgagctgg ggcaatgcgc
     1741 gatatggcag cccttttccg ccatcagtgc gccttcctgc tccgtgagat gaacggcatg
     1801 gacggcaatc aggtttgcgt ccaccagacc caggcgctgc agccgggcga gcgggcgcgt
     1861 gttgtgccgg gcgagacttt gctcgatttc atcacgcgtc tcgtgcgcgt ggatatgcaa
     1921 gggcagatcg agctcgccct gcagcatggc gatgcgctcg aatgtcttgt cgcttaccga
     1981 gtacggggcg tgcggcgcga cgcagaatga gagcaggggc tcgtacttca ggctatcccg
     2041 cattgccagg cccttgctca ggtagtcctg cgcgtcggac gcgtaagacg agggcatctc
     2101 gatcacgatg atgcccagcg ccgctcgcat ccccgcggca agcgctgcct gcgcggcggc
     2161 ctcagggtgg aagtacatgt cattgaagca cgtgacgccc ccgcgcagca tttccgcaca
     2221 agcgagcagg gtgccgtcgc gcacgaaggc ctcggatacc aggcgggtct ccactggcca
     2281 gatgtggttg tgcagccagt ccatgagagc acggtcgtcc gccaggccgc gcatcagcgt
     2341 catcgccgca tgggtgtgca ggttgatgag ccccgggatc agcgcgtgcc ccggcagctc
     2401 gatcctgcgc gcgccggggt agcggctacg cgccaattga gcgggcagca actcgcggat
     2461 aaggccggcg tcgaccacga cggcatgttc cgtcaggacc acctcgggtg gtgcgaccgg
     2521 aatgatccag ccggcttcca ctatgaaggg cgagggatta gtcaggcggg cctccagaaa
     2581 caaaaaagcc ctgcctttcg gcagggcttg gtgttgctgc gttccgagta cttacctgcg
     2641 ggcaggcttg gccttcggct tcgccttggc cttcggcttc ggcttggcca gggactgggc
     2701 gcggcccacg acttccacgg tcacgcgacg attcggcgcc aggcactcga tcagctgctt
     2761 gcgcttcatc ttgttgtcgc agaacttggt caccgggatc ggctgcttgt acgcctttcc
     2821 ttcccagaag atcaatttct ggtcgacccc cgaggctacc agggcgtcct tgacgactac
     2881 cgcgcgacgc tcggacagct tcatgttgta ttggaggccg ccgagacggt cggtatggcc
     2941 ggtcacgact acggcaccga aggtcaccgg accgcgcgcc ttggttgcct tgcgcagacc
     3001 tgccaagaac ttggagagct cggccttgtt gtcgtctgtc atctcagctt tgtcgaacgg
     3061 catgccctgc agctcgatct tttgctcgat gttgagcatc ttgggattgg ccttcttctc
     3121 cggcttgggc ttggcgtccg gcttcggctt ggctcccggc ttcggcgcgg ccgtcggggc
     3181 cttcttgacc aggtcgccat cgcactcggc aattgcgcgg gccggagtcc agtcgctggt
     3241 gcgcacgcaa acacccgcgc ccggtgcgcg gacgacgtta cccgccgagt cggtcaggta
     3301 accctgattc ttgaattggg ccgacgcggg catgggcaga aacatgccgg cagcagcgag
     3361 gccaacgagc gttatccgga ttgctgaatt catgattttt gatcctcctg tttatgcaat
     3421 ggtaagggac ccgccggaat gccggttaac ctgtggagtc tccccgggtg ctactaatat
     3481 cacaacctat taagcaaggc aaattatgcg gaaatcataa cacggttctg agtaaatagg
     3541 catatttgcc tgttgtaaat agggaactcc ctgcccccgg agggggggta tttcaccaag
     3601 tggactgggt ccgaactgcg tagtcggagg ctcggaatgg ggtggccgga gagccgcctc
     3661 gggccggtcg aaatcggcgg cccgcaggcc gcttccaaca ccgtatggcc gatgccggcc
     3721 gcgcaataaa cccgcgcgcc cagcaataac gcgcagtcgt ccggctgtct ccgatcgcaa
     3781 cctgcttttc gagcggttca gtagcgcgaa accgcgggca tgcaggcgaa gtcgggcgtg
     3841 cgcggcatgc taaaattaca cctttcaagt cgccgcgaaa taccttcctt tcttccgctt
     3901 tttcatggat cagattgcca aagaaacggt tcccgtaagt ctcgaagaag agatgcgccg
     3961 ttcctacctc gattacgcga tgagcgtgat cgtggggcgg gcgctccccg acgtgcgcga
     4021 cgggctgaag ccggtgcacc gccgcatcct gttcgcgatg catgaggcga acaacgtgtg
     4081 gaaccgcccc tacgtcaagt gcgcgcgggt ggtcggggac gtgctgggca agtaccaccc
     4141 ccatggcgac accgccacct acgaggcgct ggtgcgcatg gcgcaggact tctccatgcg
     4201 ctacaccctc atcgacggcc agggaaactt cggttcgatc gatggtgatt ccgcggcggc
     4261 ctaccgctac accgaatgcc ggctcgatcg catcacctcc gacatgctgg ccgatatcga
     4321 catggaaacg gtcgatttcg tgccgaacta cgacggcaag gagcaggagc cggccacact
     4381 gccgacccgc gtaccgaacc tgctggtgaa cggctcttcg ggcatcgcgg tcggcatggc
     4441 gaccaatatt ccgccgcaca acctgtccga ggtggtggat gcctgccagg cgctgctcaa
     4501 ggaccctgac ctcaccatcg acgatctgat caagatcgtg ccggctcccg attttccgac
     4561 acgcggcatt atctatggag tgcagggcgt caaggaggga taccgcaccg gtcgcggcag
     4621 gatcgtgatg cgcgcgcgca cccatttcga agagacggaa aagggcggac gcacctacat
     4681 cgtcgtcgac gaactgccgt accaggtcaa caaggccagc ctgctgatga agatcggcga
     4741 actcgtgcgc gagaagaaga tcgacggcat ttccgacatt cgcgacgaat ccgacaagtc
     4801 cggcatgcgc gcggtcatcg agctgaagcg cggcgagaac ccggaggtga tcctcaacaa
     4861 cctgtggaag gaaacgcagc tcgaggatac attcggcatg aatatggtcg cgctggtgga
     4921 tggccagccg
//
LOCUS       DBNGSG010000330         3883 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3332932, whole genome shotgun sequence.
ACCESSION   DBNGSG010000330 DBNGSG010000000
VERSION     DBNGSG010000330.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3883)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3332932"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="AC158Q_08615"
     CDS             <1..687
                     /locus_tag="AC158Q_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactarate dehydratase"
                     /protein_id="HIR3339878.1"
                     /translation="CGSNPTVGNAFDKLEPLGATLVFGETTEITGGENIVADRCATPQ
                     VREEWMRVFNRYQDVINRHKTNDLSESQPTKGNIAGGLTTIEEKALGNIQKIGKKCKV
                     IGILDKAEAPTRPGLWFMDSSSAAAEMVTLVAAAGYAVHFFPTGQGNVIGNPILPVIK
                     LTANPRTARTMSEHIDYDCSGLLQRQKNLDQTGDELLEVMLRTCNGRLTAAEALGHRE
                     FVLTRLYESA"
     gene            817..2463
                     /locus_tag="AC158Q_08620"
     CDS             817..2463
                     /locus_tag="AC158Q_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR3339879.1"
                     /translation="MAQLRGADILARALAQAGARQLFSLSGNHIMTVYDAALDARLAI
                     THVRHEGAAVHMADAWGRLTGNPGIALLTGGPGHANGVGALYTALAAESPLVMLSGHA
                     PLAELGRGSFQEMAQADLAAPVAKASWTAQRAETLDEDIARAMRIARSGRPGPVHVSL
                     PFDLLEAKVAGVAGVPDAAFRPIVQPLTDAAADETLAELERAQRPLIIVGPVMANARA
                     LRERLAAATGAPVICMESPRGLADPGLGDFAEVLRQSDLVVLLGKQPDYSIRFGAAMA
                     ANCRFLVVDPAVEVLARARTTLGADRVALAAVADSQAAAETLASRAGREPGATAWAGE
                     VEAAVRYRPAEWARIASRAGGPVHPIEVGRAAQKLVERSPDTVLVVDGGEFGQWAQAC
                     ITAPTRLTNGVAGSIGSAIPFALAARAARPGAPVVALLGDGTFGFHMAEFDTAARARL
                     PFVAIVGNDACWNAEHQIQLRAYGRERAHGCELAPARYDQAVAALGGHGECVTRAEQL
                     PGALERAAASGKPACVNVMIERLPAPVMARAPAASAGAVH"
     gene            2463..3176
                     /locus_tag="AC158Q_08625"
     CDS             2463..3176
                     /locus_tag="AC158Q_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HIR3339880.1"
                     /translation="MDTLHVSVWRGGESGAFQAFEVPRRESQTVLDVVTYIQRALDPT
                     LAYRFACRVGVCGSCAMTVNGRARWTCRTHVDKVAQGGRIEIAPLNNLPVVRDLVTDM
                     TEFFDKWARARGRFRGAATRKDDFARVMPSSSERRLADAGIECIGCGVCYSSCDVVSW
                     KPDYLGPAALNRAWTLVNDVRDTDNRARLAAVAGDAGCHACHTHMSCSERCPKQISPT
                     AAIAGLKRAAALAALKGEI"
     gene            3283..3621
                     /locus_tag="AC158Q_08630"
     CDS             3283..3621
                     /locus_tag="AC158Q_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HIR3339881.1"
                     /translation="MSARSQTLLWLAQRASAVVLAVCVVVHLVTIIYAVRHGLTAAEI
                     LGRTRGNAAWAAFYALFVAAAAVHAPIGLRNFLDETFNWRGGSRDVVVLAAALALAML
                     GLRAVYAVFI"
     gene            3741..>3883
                     /locus_tag="AC158Q_08635"
     CDS             3741..>3883
                     /locus_tag="AC158Q_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HIR3339882.1"
                     /translation="MKNDFRARNHPAYWAFIVHRASGVLLALFLPLHFWALGRALQGE
                     AKL"
BASE COUNT          589 a         1333 c         1369 g          592 t
ORIGIN      
        1 tgcggttcca acccgacggt aggcaacgca ttcgacaagc tcgagccgct cggcgcgacg
       61 ctggtcttcg gcgagaccac cgagatcacc ggcggcgaga acatcgtcgc cgaccgctgc
      121 gcgacgccgc aagtgcgcga ggagtggatg cgggtgttca accgctatca ggacgtgatc
      181 aatcgccaca agaccaacga cctgtccgag tcgcaaccga ccaagggcaa tatcgccggc
      241 gggctcacca cgatcgagga aaaggcgctc ggcaacatcc agaagatcgg caagaagtgc
      301 aaggtgatcg gcatactcga caaggccgag gcgccgaccc ggccgggcct gtggttcatg
      361 gactcgtcgt cggccgcagc ggaaatggtg acgctggtcg cggcggcggg ctacgccgtg
      421 catttctttc ccaccgggca gggcaacgtc atcggcaacc cgatcctgcc ggtgatcaag
      481 ctgaccgcca atccgcgcac cgcgcgcacg atgtcggagc acatcgatta cgattgttcg
      541 ggcctgctgc agcggcagaa gaacctcgac cagaccggcg acgaactgct cgaagtgatg
      601 ctgcgcacct gcaatggccg gttgaccgca gcggaagccc tcggtcatcg cgagttcgtt
      661 ctcacgcggc tctacgagag cgcgtgagga cccagggatg aaggcggaag tcagaaggca
      721 gaattgccga accgcgcacg gcgcggaagc gcgccggggc gattggcttc atccttcatc
      781 tttccgcctt ctgcctcgtg ccgcgcgcgc gcgtctatgg cgcagctgag aggcgcggat
      841 atcctcgccc gcgctctggc acaggcgggc gcgcgacagc tgttctcgct gtccggcaac
      901 cacatcatga ccgtgtacga cgccgcgctc gacgcgcggc tcgcgatcac gcacgtgcgc
      961 cacgagggtg ccgcggtaca tatggccgac gcctggggcc ggcttaccgg caacccgggc
     1021 attgcgctgc tgaccggggg gcccggccac gccaacggcg tcggcgcgct ctacaccgcg
     1081 ctcgcggccg agtcacccct ggtgatgctg tccggccatg ccccgctggc cgaactcggc
     1141 cgcggctcct tccaggaaat ggcgcaggcc gatctcgccg cgccggtcgc caaggcgtcg
     1201 tggacggcgc aacgcgccga gacgctggat gaggacatcg cgcgggccat gcgcatcgcg
     1261 cgctccggtc ggcccggccc ggtgcacgtc agcctgccgt tcgacttgct ggaggcgaaa
     1321 gttgccggcg tggccggcgt gccggacgcc gcgttccggc cgatcgtcca gccgctcacg
     1381 gatgccgcgg ccgacgagac gctcgcggaa ctcgagcgcg cgcagcggcc gttgatcatc
     1441 gtggggccgg tgatggcgaa cgcgcgcgca ctgcgggagc ggctcgccgc ggcgaccggg
     1501 gcgcccgtca tatgcatgga aagcccgcgc ggcctcgccg accccggcct gggcgatttc
     1561 gcggaggtgc tcaggcaatc cgatcttgtc gtgctgctcg gcaagcagcc cgattactcg
     1621 attcgtttcg gcgcggcgat ggccgcgaac tgccgctttc tcgtggtcga ccccgccgtc
     1681 gaagtgctgg cgcgcgcgcg aacgacgctc ggcgcggatc gcgtcgcgct tgcggcggtc
     1741 gcggattcgc aggcggcggc cgagacgctg gcctcgcgcg ccggccggga gccgggcgca
     1801 accgcctggg ccggggaggt ggaagccgca gtgcgctacc gtccggccga gtgggcgcgg
     1861 attgcctcgc gcgccggggg cccggtgcat ccgatcgagg tgggacgcgc agcgcaaaaa
     1921 ctcgtcgagc gctcgcccga cacggtgctc gtggtggacg gtggcgagtt cgggcagtgg
     1981 gcgcaggcct gcatcaccgc gccgacgcgg ctcaccaacg gcgttgccgg ctccatcgga
     2041 tcggcaattc cattcgctct cgcggcgcgc gccgcgaggc ccggtgcccc ggtcgtcgcg
     2101 ctgctgggcg acggcacgtt cggtttccac atggcagagt tcgacaccgc ggcgcgggcg
     2161 cggctgccgt tcgtcgcgat cgtcggcaac gacgcctgct ggaacgccga gcaccagatc
     2221 cagctccggg cgtacgggcg cgaacgcgct cacggctgcg aactcgcgcc cgcgcgctac
     2281 gaccaggccg tcgccgcgct gggcgggcac ggcgagtgcg tcacgcgcgc ggagcagttg
     2341 cccggcgcgc tcgagcgcgc cgcggcgtcc ggcaagcccg cgtgcgtgaa cgtgatgatc
     2401 gagcggctgc cggcgccggt catggcccgc gccccggcgg cgagcgcggg cgcggtgcat
     2461 tgatggatac gctgcacgtc agcgtctggc gcggcggcga gtcgggcgca ttccaggcat
     2521 tcgaggtgcc gcggcgggag agccagacgg tgctcgacgt cgtcacttac atccagcgcg
     2581 cgctcgaccc cacgctcgcc taccggttcg cctgcagagt cggcgtgtgc ggctcgtgcg
     2641 cgatgacggt gaacggccgc gcgcgctgga cctgccgcac gcacgtcgac aaggtggcgc
     2701 agggcggccg catcgagatc gcgccgctca acaacctgcc ggtggtgcgc gacctcgtca
     2761 ccgacatgac ggaattcttc gacaaatggg cgagggcgcg cggccgcttt cgcggcgcgg
     2821 ccacgcgcaa ggatgatttc gcgcgcgtca tgccgtcatc ctcggaacgc cggctcgcgg
     2881 atgccgggat cgagtgcatc ggctgcggcg tgtgctacag ctcgtgcgac gtcgtgtcct
     2941 ggaagccgga ctacctcggg cccgccgcgc tgaaccgcgc ctggacgctg gtgaacgacg
     3001 tgcgcgatac cgacaaccgc gcgcggcttg ccgcggtggc cggcgatgcc ggctgccacg
     3061 cgtgccacac gcacatgagc tgcagcgagc gctgcccgaa gcaaatctcg cccaccgccg
     3121 ccatcgcggg cctgaagcgc gccgccgctc tcgcggcgct caagggcgag atttgaaggc
     3181 acaaggcaag aggcaagagt caaaagtgca ggaccccacc gcgcgcgttc ttcggctact
     3241 tgtgccttgt gccttgagcc ttgtgccttc ggagcacacg gcatgagcgc gcgctcgcag
     3301 acgctgctgt ggctggcgca gagagcaagc gcagtggtgc ttgccgtgtg cgtggtcgtg
     3361 catctcgtca ccatcatcta cgcggtgcgc cacgggctca ctgcggcgga gatactcggg
     3421 cgcacgcgcg gcaacgcggc gtgggcggcg ttctacgcgc tgttcgtggc cgccgccgcc
     3481 gtgcatgccc cgatcgggct gcgcaacttt ctcgacgaaa ccttcaactg gcgcggcggc
     3541 agccgcgacg tcgtggtgct ggcggccgcg ctggcgctcg ccatgctggg cttgcgcgcg
     3601 gtctacgccg tttttatttg aaaccgccgg tgaacgcaga tgaacgcaaa aaacaccagc
     3661 gaaaatcacg gcagcccgcc gggtttgtgt ccggttttca tctgcgtata tctgcgttta
     3721 tctgcggttg catctgaatt atgaaaaacg acttccgcgc gcgcaaccat cccgcctact
     3781 gggctttcat cgtccaccgc gcctccggcg tgctgctcgc gctgttcctg ccgctgcatt
     3841 tctgggcgct gggccgggcg ctgcagggcg aggcaaagct cga
//
LOCUS       DBNGSG010000331         5390 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2484612, whole genome shotgun sequence.
ACCESSION   DBNGSG010000331 DBNGSG010000000
VERSION     DBNGSG010000331.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5390)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5390)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5390
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2484612"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /gene="tolA"
                     /locus_tag="AC158Q_08640"
     CDS             complement(<1..742)
                     /gene="tolA"
                     /locus_tag="AC158Q_08640"
                     /inference="COORDINATES: protein motif:HMM:NF024503.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HIR3339883.1"
                     /translation="MTAYAYETPNDKAISGAAALVMHALFLALLIFGVNWKKPQPAAM
                     VAELWSNLPPVAAPRPEPPPPPKVEPEPPKPQPRVEPKPVPKVEPKPQPKAEIDLREK
                     QEKERKAKEQAALEKKKREEKALAEKKKREEQQRLEALKRQQAKEAEAKRLAQEQADA
                     QRKLAQQQAAAQAREVDKYRAAIAARIRRFIVEPPNIQGNPVTELDIIMLPGGEVLDV
                     RTKKPSGQTAWDNACERAIRRAQPLPLPS"
     gene            complement(905..1297)
                     /gene="tolR"
                     /locus_tag="AC158Q_08645"
     CDS             complement(905..1297)
                     /gene="tolR"
                     /locus_tag="AC158Q_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HIR3339884.1"
                     /translation="MNQINVVPYIDVMLVLLVIFMVTAPLINPGQIELPTVGKTSDPP
                     QQPMEVSVRSDGTLTLRDRARSMDERKVTRNDLLAAIRQKQQQNPEQAVVISADKDVR
                     YEAVLEVMDMLQQNQVRKVGLLAKPRGG"
     gene            complement(1445..2104)
                     /gene="tolQ"
                     /locus_tag="AC158Q_08650"
     CDS             complement(1445..2104)
                     /gene="tolQ"
                     /locus_tag="AC158Q_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HIR3339885.1"
                     /translation="MSVLSLITGASVLVQIVMLSLLLASLFSWYYIFLKFFTFSRATR
                     QADRFEKQFWSGVDLNELYQRAINARGSAGSQERIFESGFQEFVKLRKQPGMDIRTVL
                     DGTRRAMRATYQRELERLESYLSFLASVGSVSPYVGLFGTVWGIMNSFRGLANMSQAT
                     LGAVAPGIAEALIATAMGLFAAIPAVVAYNHFAGDVNRLAIRFDNFMEEFSNILQRQA
                     K"
     gene            complement(2125..2574)
                     /gene="ybgC"
                     /locus_tag="AC158Q_08655"
     CDS             complement(2125..2574)
                     /gene="ybgC"
                     /locus_tag="AC158Q_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HIR3339886.1"
                     /translation="MSSRKPSATQHSTLNTQHFSWPVRVYYEDTDLGGIVYYANYLRF
                     LERARTEWLRALGFEQSALAAVHQVVFVVRSISLDFLKPSRIDDTLDITVSLREAGAS
                     RIALSQFVRRGAEDLVAAQVELACVNTATFRPVRIPGPVIARIGRKT"
     gene            complement(2614..3660)
                     /gene="ruvB"
                     /locus_tag="AC158Q_08660"
     CDS             complement(2614..3660)
                     /gene="ruvB"
                     /locus_tag="AC158Q_08660"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HIR3339887.1"
                     /translation="MIETDRLISAAPVTPQEEALERTLRPRVLEEYVGQEKIRGQLTV
                     FIEAARKRKEALDHVLLFGPPGLGKTTLAHIIAREMGVSLRHTSGPVLERPGDLAAIL
                     TNLEPNDVLFIDEIHRLSPVVEEILYPALEDYQIDIMIGEGPAARSVKLDLPPFTLVG
                     ATTRAGMLTNPLRDRFGIVSRLEFYTQEELARIVQRSAKLLEIETAAEGAREIASRSR
                     GTPRIANRLLRRVRDFAEVRAKGRITREVADAALKMLDVDALGLDVMDRKLLLAVIEK
                     FAGGPVGVENLAAAIGEERDTIEDVLEPYLIQQGYLQRTLRGRVATVTAYRHFGLAQP
                     ARAGTPDLWEAPKE"
     gene            complement(3653..4045)
                     /locus_tag="AC158Q_08665"
     CDS             complement(3653..4045)
                     /locus_tag="AC158Q_08665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR3339888.1"
                     /translation="MKHTDLVVWQKAMDLVTEVYKLSESFPVRENYGLTSQLQRAAVS
                     IPSNIAEGHGRKSTQAYLNHLSIAHGSLMEVETQVEIARRLGYLSAHPAQLVTERTAE
                     VGRMLNALQRALVVRKKSPHPESRILHD"
     gene            complement(4081..4659)
                     /gene="ruvA"
                     /locus_tag="AC158Q_08670"
     CDS             complement(4081..4659)
                     /gene="ruvA"
                     /locus_tag="AC158Q_08670"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HIR3339889.1"
                     /translation="MIGRIAGKLLEKHPPQIVVDVHGVGYELYVPMSTFYQLPATGSE
                     VSLVTHLVVREDAHHLYGFATEAERHAFRQLLKISGVGARTALSVLSGLSVSDLREAV
                     SSQDGGRLTKVPGIGKKTAERLLLELRGKLDAVSIASAAARGDGQAGDITNALLALGY
                     NDREASWVIKQLPAGLSVADGIRQALRLLSKQ"
     gene            complement(4656..>5390)
                     /locus_tag="AC158Q_08675"
     CDS             complement(4656..>5390)
                     /locus_tag="AC158Q_08675"
                     /inference="COORDINATES: protein motif:HMM:NF016027.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3339890.1"
                     /translation="RLANSDEAVLRAINSALPAGTPRLSALHDIFDGARCFFNGVPEL
                     DPYGPRDPGGFLGLYSGGVGSAVPQWPAGEAPRVFAYLHSEYRHLDTALAALAASGAR
                     CVVYLFGSTPEIRQKYAGPSVVFSTELLDIDQAVQAADLCVCHANFGTVMAVLRGGKP
                     LLLLPYDLEKFLSSASLEKLGVGRMVRPEGELVDIGGTLAQMLGSPAFTEAAKAFALG
                     HREPSVDTIIAGAARRIEALAQGQPG"
BASE COUNT          886 a         1787 c         1735 g          982 t
ORIGIN      
        1 cggagggcag cggcaacggc tgcgccctgc ggatcgcgcg ctcgcacgca ttgtcccagg
       61 ccgtctgacc gctcggcttt ttggttctta cgtcgagaac ctcgccgccg ggcagcatga
      121 taatgtcaag ttcggtgacc ggattgccct gaatgttggg cggctcgacg atgaagcgcc
      181 ggatgcgcgc ggcgatcgcc gcccggtact tgtcgacctc ccgcgcctgc gctgccgcct
      241 gctgctgggc gagctttctc tgcgcgtccg cctgctcctg cgccaggcgc ttcgcttccg
      301 cctccttcgc ctgctgccgc ttcagcgcct cgaggcgctg ctgctcttcc cgctttttct
      361 tctcggcgag agccttctcc tcgcgctttt tcttctcgag cgccgcctgc tctttcgcct
      421 tgcgctcctt ctcctgcttc tcgcgcaagt cgatctcggc cttcggctgg ggcttcggtt
      481 cgaccttcgg gaccggcttc ggctcgaccc tgggctgcgg cttgggcggc tcgggctcca
      541 ccttcggcgg cgggggcggc tcgggcctgg gcgccgctac cgggggcagg ttgctccaca
      601 actccgccac catcgcggcg ggctgcggtt tcttccagtt gacgccgaaa atcagcagcg
      661 ccaggaacag cgcgtgcatc accagcgcgg ccgcgccgga gatcgccttg tcgttgggtg
      721 tctcgtacgc gtacgccgtc atgagaaccg ctcaatgacc gttaccagaa gtcatcgcaa
      781 aaactgctgt aaccaccgac acacacggag ccacacagaa attcttgaat gttttcaggg
      841 atgttatccg ggtcggtcgg gtccgtgtca ttccatgtga ttccggggaa ttttgagacc
      901 gggcctagcc gccgcgcggc ttggcgagca gccccacctt gcgcacctgg ttctgctgca
      961 gcatgtccat gacttccagc acggcctcgt agcgcacgtc cttgtcggcg gaaatcacca
     1021 cggcctgctc gggattctgc tgctgcttct ggcggatcgc ggcgaggaga tcattgcgcg
     1081 tgaccttgcg ctcgtccatc gagcgggcgc ggtcacgcag cgtcagcgtg ccgtcggacc
     1141 gcaccgagac ctccatcggc tgttgcgggg gatcggaggt cttgccgacc gtgggcaact
     1201 cgatctgccc ggggttgatg agcggcgcgg tgaccatgaa gatcaccagg agcaccagca
     1261 tcacgtcgat gtacggcacg acgttgattt ggttcatgag cctgcgcccg gagcggcgct
     1321 ctatgagcac ggagtggccc tccgtgcaga gagggggaat gagtgaggtg tgaggagcaa
     1381 gcaccccgac cggcttgtca tgattgtcgc ctcactcctc acatctctct cctcacgcct
     1441 cactctactt ggcctgccgc tggaggatgt tggagaactc ctccatgaaa ttgtcgaagc
     1501 ggatggccag ccggttgacg tcgccggcga agtggttgta cgccaccacc gccgggatcg
     1561 ccgcgaacag ccccatggcg gtcgcgatca gcgcctccgc gatgcccggc gccaccgcgc
     1621 cgagcgtcgc ctgcgacatg ttggcgagcc cgcggaacga attcatgatc ccccacaccg
     1681 tgccgaacag cccgacgtag gggctcaccg accccaccga ggcgaggaac gagaggtacg
     1741 actcgagccg ctccagctcg cgctggtaag tcgcccgcat cgcgcgccgc gtgccgtcca
     1801 gcacggtgcg gatgtccatg ccgggctgtt tcctcagctt cacgaactcc tggaagccgg
     1861 actcgaagat gcgctcctgg ctgcccgcgg aaccgcgcgc gttgatcgcg cgctggtaga
     1921 gctcgttcaa gtcgaccccg ctccagaact gcttctcgaa ccggtcggcc tggcgggtgg
     1981 cgcggctgaa ggtgaagaac ttgagaaaga tgtaatacca ggaaaagagc gaggcgagca
     2041 gcagcgacag catgacgatc tgcaccagca cgctcgcgcc ggtgatgagc gagagcaccg
     2101 acatgtcatg ggttacggtg ggattcacgt ttttcttcct atcctggcga tgaccggccc
     2161 gggtattcta acgggcctga acgtggccgt gttgacgcag gcgagctcca cctgcgccgc
     2221 gaccaggtct tccgcgccgc gccgcacgaa ctgcgacagc gcgatgcggc tggcgccggc
     2281 ctccctcaac gagacagtga tatcgagcgt gtcgtcgatt cgcgacggtt tcaggaaatc
     2341 cagcgatatc gaccgcacca cgaacacgac ctgatggaca gccgccagcg ccgattgttc
     2401 aaagcccagc gcccgcagcc actcggtgcg cgcgcgctcg aggaagcgca ggtaattcgc
     2461 gtagtagacg atgccgccca ggtcggtgtc ctcgtagtag acgcggaccg gccaagagaa
     2521 gtgttgagtg tttagtgttg agtgttgagt tgcggacggc ttgcgcgagc tcatcggggc
     2581 gaaacccact taacactcaa aactcaacac ttatcactcc tttggggctt cccaaaggtc
     2641 cggcgtgccg gcgcgggcgg gctgcgccag gccgaagtgg cggtacgccg tgacggtggc
     2701 gacgcgcccg cgcagcgtgc gctggagata gccctgctgg atgaggtagg gctcgagcac
     2761 gtcctcgatg gtgtcgcgct cctcgccgat cgccgcggcg aggttctcga cgccgaccgg
     2821 cccgccggcg aatttctcga tcacggcgag caggagcttc ctgtccatga cgtcgagccc
     2881 gagcgcgtcc acgtcgagca tcttcagggc ggcgtccgcc acctcgcgcg tgatcctgcc
     2941 tttcgcccgc acttccgcga aatcgcgcac gcgccgcagc aggcggttgg cgatgcgggg
     3001 cgtgccgcgc gagcggctgg cgatctcgcg cgcgccttcc gcggcggtct cgatctcgag
     3061 cagcttcgcc gagcgctgca cgatgcgcgc gagctcctcc tgcgtgtaga actccagccg
     3121 cgagacgatg ccgaagcggt cgcgcagcgg gttggtcagc atgccggcgc gcgtggtcgc
     3181 gccgaccagg gtgaacggcg gcaggtcgag cttgaccgag cgcgccgccg ggccctcgcc
     3241 gatcatgatg tcgatctggt agtcctcgag cgcggggtaa agaatctcct ccaccaccgg
     3301 cgagaggcgg tggatctcgt cgatgaacag cacgtcgttc ggctcgaggt tggtgaggat
     3361 cgccgcgagg tcgcccgggc gctccagcac cgggcccgag gtgtgccgca ggctcacgcc
     3421 catctcgcgc gcgatgatgt gggcgagcgt ggtcttgccg aggcccggcg gcccgaacaa
     3481 cagcacgtgg tcgagcgctt ccttgcgctt gcgcgcggcc tcgatgaaga cggtgagctg
     3541 gccgcggatc ttctcctgcc ccacgtactc ctcgaggacc ctggggcgca gcgtgcgctc
     3601 gagggcctct tcctgcggcg tgacgggcgc ggcggagatg aggcggtcgg tttcaatcat
     3661 gcaggattcg ggattcagga tgcggggact tcttgcgcac aaccagcgct cgctggagcg
     3721 cgttcaacat tcttcccact tcagctgtac gctccgttac cagttgtgcg gggtgcgcac
     3781 tcaaataccc caagcgtctt gcaatctcga cttgagtttc gacctccatc agggatccgt
     3841 gagcgatcga caagtgattg aggtaggcct gcgtcgactt gcgaccatgg ccttccgcga
     3901 tattggacgg aatggaaacc gccgcccgct gcagctgact cgtcaatccg taattctcgc
     3961 gaacggggaa tgattcggac aatttataga cctcggtgac cagatccatc gccttctgcc
     4021 acacaaccag atcggtgtgt ttcatgggcc ctccgaatcc tgaatcccga ctcccgaatc
     4081 ctattgcttc gatagaagcc tgagcgcctg gcgtatgccg tccgcgacgc tgagtcccgc
     4141 cggcaactgc ttgattaccc agctcgcctc gcgatcatta tagccgagcg cgagcagcgc
     4201 attcgtgatg tcgccggcct gtccgtcgcc tctcgccgcg gcggacgcga tcgacaccgc
     4261 atcgagcttg ccgcgcagct ccagcagcag gcgttccgcg gtcttcttgc cgatgccggg
     4321 caccttggta agacggccgc cgtcctggga gctgaccgcc tcgcgcaggt cggaaaccga
     4381 aagccccgag agcaccgaca gcgcggtgcg ggcgccgacg ccggagatct tcagcagctg
     4441 ccggaacgca tggcgctccg cctcggtcgc gaaaccgtag aggtggtgcg cgtcctcgcg
     4501 cacgacgagg tgcgttacga gcgacacctc cgagccggtc gcgggcagct ggtagaaggt
     4561 gctcatcggc acgtagagct cgtagccgac cccgtggacg tccacgacga tctggggcgg
     4621 atgtttctcg agcagcttgc cagcaatgcg gccgatcatc ccggttgtcc ttgcgcgagc
     4681 gcttcgatgc gccgcgctgc gcctgcaatg attgtatcaa ccgagggctc gcggtgcccc
     4741 agcgcgaacg ctttggccgc ctccgtaaag gccgggctcc cgagcatctg cgcgagcgtg
     4801 ccgccgatgt ccaccaactc cccttccggg cgcaccatcc tgccgacacc cagtttttcc
     4861 aggctggcgg acgacaggaa cttctccagg tcgtagggca acagcaacag cggcttgccg
     4921 ccgcgcagca cggccatgac cgtgccgaaa tttgcgtggc aaacgcacaa gtcggccgct
     4981 tgcaccgctt gatcgatgtc caggagttcc gtgctgaata cgacgctggg ccccgcgtac
     5041 ttctggcgga tttccggcgt gcttccaaaa agataaacca cgcagcgcgc gccactggcc
     5101 gccagggcgg ccagtgccgt gtcgaggtgc cggtactcgc tgtgaaggta ggcgaaaacc
     5161 cggggggcct cgcccgcggg ccactggggc acggcagatc cgactccgcc gctatacagg
     5221 ccgaggaatc cgccgggatc ccgcgggcca taaggatcca gttccgggac accgttgaaa
     5281 aaacagcgcg cgccgtcaaa aatgtcgtgc agcgcgctca gccgcggcgt gccggcagga
     5341 agagccgaat tgatcgccct gagcaccgct tcatcgctat tggccagccg
//
LOCUS       DBNGSG010000332         3017 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1636002, whole genome shotgun sequence.
ACCESSION   DBNGSG010000332 DBNGSG010000000
VERSION     DBNGSG010000332.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1636002"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="AC158Q_08680"
     CDS             <1..1016
                     /locus_tag="AC158Q_08680"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HIR3339891.1"
                     /translation="LAGLFLVADDEEDAARLPRDEFDLPLVIQDRRFNAANQLVYLAP
                     GMMDRMMGFSGDRVLVNGSADYTRPVARTAYRLRVLNASNSTLYRLRWSNGRPLTLIG
                     TDGGLLERPVTHASLTLGIGERADVWVDFAGEQPGDEIALVTESWSPSLENITGLQTG
                     AEPVQGGRPIARFRIANGSAQKSALPQRLSSFPALRIEEAVNRARPRTIRLSMQRGQV
                     MLNGRAFGRMDEVADDEKVKLGTTEVWEFANDASFGMRMAHPMHVHNLQFRVIGRTGA
                     DGHPLSAGFTDEGLKDVVLVLPGERVRVLMKFEDHTGIYLYHCHILEHEDLGMMRNYL
                     VQA"
     gene            1229..1588
                     /locus_tag="AC158Q_08685"
     CDS             1229..1588
                     /locus_tag="AC158Q_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339892.1"
                     /translation="MTSRFGKLVLALALAVMPLQGVAATLSVLLCHGEAQVPAAHAGD
                     SHDHAAHADGQHDGHPADGSAAGGSVFHLCCHFTVSAPAAVTLAVALPDFPALAFAPT
                     PLHDLFIPDRPQRPPLA"
     gene            1703..2023
                     /locus_tag="AC158Q_08690"
     CDS             1703..2023
                     /locus_tag="AC158Q_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339893.1"
                     /translation="MKSYFALATLIMALAAHASAQQPAATPHPADPAAPVPATKYESA
                     FTGYRAFREEPVSPWRGVNDEVARAGGHVGIVGGAGRAGQVHGSGAAGPAAPPAQSAP
                     GSHK"
     gene            2037..>3017
                     /locus_tag="AC158Q_08695"
     CDS             2037..>3017
                     /locus_tag="AC158Q_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR3339894.1"
                     /translation="MKLSFLIVPAAFLLGGCATLSPDGGFGTVQSAARERGITQETRW
                     IRSETDAGNARAAVKKLLAAPLTADAAVQIALANNRGLQATYADLGIAETDVVQAGRL
                     RNPGFSYGRFTRADEVEIERTFLFELLGLLTMPIRTDLEKRRYELTKGRVAGEIMQVA
                     ADTRRAWVSAVSAQEAVRYREQVKSAAEASAELARRMAAAGNFSKLDQAREQVFYGEA
                     TAELARARQAALSERERLTRMMGLWGEDVNFSLPARLPDLPKSAREIADLEAQALKQR
                     LDVQGAMQEAENVAASLGLTQATGFISVLEVGYQRTSETGQPRKSGYEIEI"
BASE COUNT          509 a         1028 c          995 g          485 t
ORIGIN      
        1 gcctggccgg actgttcctc gtcgcggacg acgaggagga tgccgcgcgg ctgccgcggg
       61 acgaattcga cctgccgctg gtcatccagg accggcgatt caatgccgcc aaccaactcg
      121 tctaccttgc gcccggcatg atggatcgca tgatgggctt cagcggcgat cgcgtgctcg
      181 tcaacggttc ggccgattac acgcggcccg tggcgcgcac cgcgtaccgg ctgcgtgtgc
      241 tgaatgcatc gaattcgacg ctctatagac tgcgctggag caatggccgg ccgctgaccc
      301 tcattggcac ggatggcggc ctgctggagc gccccgtgac gcacgcgtcc ctcaccctcg
      361 ggatcggcga acgggcggac gtctgggtgg acttcgcagg cgagcagccg ggtgacgaaa
      421 tcgctctcgt aaccgagtcc tggtcgccat cgctcgaaaa catcacgggc ctgcaaaccg
      481 gcgcggagcc cgttcagggc gggcggccga tcgcccggtt ccggatcgcg aacggcagcg
      541 cgcaaaaatc ggcgctgccg cagcggttgt cctccttccc ggcgctcagg atcgaagagg
      601 cggtcaaccg cgcccggccg cggacgatcc ggctgtcgat gcagcgcggc caggtgatgc
      661 tgaacggccg cgcattcggc cgcatggacg aggttgccga cgatgaaaaa gtgaagctcg
      721 gcacgaccga agtatgggaa ttcgccaacg acgcgtcgtt cggcatgcgg atggcgcacc
      781 cgatgcacgt tcacaacctg caattccggg tcatcgggcg caccggggct gacggccacc
      841 ccctgagcgc cggctttacc gatgaaggcc tgaaggacgt cgtgctcgtg ctgccgggag
      901 aacgcgtgag agtattgatg aagttcgagg atcacaccgg gatctatctc tatcattgcc
      961 acatcctcga gcatgaagac ctcggaatga tgagaaacta ccttgtccag gcctgattcc
     1021 gattgcgcca tcatggatgc actggctcga agaattgtcc ggcatctgcg gcaggaaacc
     1081 gggccggtta aacccggtgt cgcgcgccga ctgcaggaaa tccggcaaca ggtgctccgg
     1141 cagcggcgcg atggaacttc cgccgcttcg cggggtcact cgagatgaat tgcggcgttg
     1201 caacgaaagt ggttacactt cggccctgat gacaagccgg ttcggaaaac tcgtcctcgc
     1261 gttggccctt gcggtgatgc cgctgcaagg cgttgcagcg accttgtcgg tcctgctttg
     1321 ccacggcgag gcgcaggtgc ccgccgcgca tgcgggagac agccacgatc acgccgcgca
     1381 cgcggacggc cagcacgatg gccaccccgc cgacggatcc gccgcgggcg gctctgtttt
     1441 tcacctgtgc tgccatttca ccgtctccgc gccggcagcc gtgacgctgg cggtggcgtt
     1501 gcccgatttc ccggcgctgg cttttgcgcc aacccctctg cacgacctgt tcattcccga
     1561 ccggccccaa cgcccgcctc tcgcctgacc ttcgtcgctc gcgggtaatc cgcgtttgaa
     1621 gcgcgcctcg ctgtcgtttt tttcgcgcgc catcgtcgca ccgcccacgc gcggcgcggc
     1681 aacttccttg accagggcag gcatgaaatc gtactttgca ttggcaacac tcatcatggc
     1741 cctcgcggcg cacgcctccg cccagcagcc ggcggccacg ccgcatcccg ccgacccggc
     1801 cgcgccggtg ccggcgacta aatacgagtc cgcctttacc ggctaccgcg catttcgcga
     1861 ggagccggtc tcgccctggc gcggcgtgaa cgacgaagtt gcccgcgccg gcggccacgt
     1921 gggcatcgtc ggcggagccg gccgagctgg gcaggttcac ggcagcggag ccgccgggcc
     1981 agcagccccg ccagcgcaat ctgcgcccgg ctcccacaag taataggaat caggccatga
     2041 agctttcctt tctgatcgtt cctgcggcgt ttttgctcgg cggttgcgcc acgctgtcgc
     2101 ccgatggcgg cttcggcacg gtgcaatcgg ccgcccggga gcgtggcatc acgcaggaga
     2161 cccgctggat tcgcagcgag accgacgccg ggaacgcgcg cgccgccgtg aaaaagttac
     2221 tcgcggcccc gctcaccgcg gatgccgccg tgcagattgc gctcgccaac aaccgcggcc
     2281 tgcaggcaac ctacgccgat ctgggcatcg ccgaaaccga cgtggtgcag gccggccgcc
     2341 tgcgcaaccc gggcttctcc tacggccgtt tcacccgcgc cgacgaagtg gagatcgagc
     2401 gcaccttcct gttcgaactg ctcgggctcc tgacgatgcc gatccgcacg gacctcgaga
     2461 agcgccgcta cgagctgacc aagggccgcg tagccggcga gatcatgcag gtcgccgccg
     2521 acacgcggcg cgcctgggtg agcgcggtgt ccgcgcagga ggcggtgcgg tacagggaac
     2581 aggtgaagag cgcggcggaa gcgagcgcgg agctggcccg gcgcatggcg gccgccggca
     2641 acttcagcaa gctcgaccag gcccgcgagc aggtttttta cggcgaggcg accgcggagc
     2701 tcgcgcgcgc aagacaggcc gccctctccg agcgtgagcg gttgacccgg atgatggggc
     2761 tgtggggcga ggacgttaac ttcagtcttc cggcccggct ccccgacctg ccgaagtcgg
     2821 cgcgcgagat cgccgatctc gaggcgcagg ccctgaagca acgtctcgac gtgcagggcg
     2881 ccatgcagga agcggaaaat gtcgctgcct ccctcggtct cacccaggcg accggcttca
     2941 tcagcgtgct cgaagtgggg taccagcgca ccagcgaaac cggccagccg cgcaagtcgg
     3001 gctacgagat cgaaatc
//
LOCUS       DBNGSG010000333         4294 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1128037, whole genome shotgun sequence.
ACCESSION   DBNGSG010000333 DBNGSG010000000
VERSION     DBNGSG010000333.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4294)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1128037"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..578
                     /locus_tag="AC158Q_08700"
     CDS             138..578
                     /locus_tag="AC158Q_08700"
                     /inference="COORDINATES: protein motif:HMM:NF012342.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HIR3339895.1"
                     /translation="MERMQKGFTLIELMIVVAIIGILAAVAIPAYQDYLARSQMSEAV
                     NLLGSGKTPLAEYFADKGTWPAALSDVMGNTTGKYTGSIVMTGTPTVSGTASAYTLAA
                     SMKTSTINSAITGKTVYLSTTDSGRSWNCAAGDVDVRYLPGACR"
     gene            943..1383
                     /locus_tag="AC158Q_08705"
     CDS             943..1383
                     /locus_tag="AC158Q_08705"
                     /inference="COORDINATES: protein motif:HMM:NF012342.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HIR3339896.1"
                     /translation="MKSLQKGFTLIELMIVVAIIGILAAVAIPAYQDYIARSQMSEAV
                     NLLGSGKTPLAEYFADKGTWPGALADVMGNTSGKYTGSITTTGTPTVNGTASAYTLAA
                     SMKTSTINSAITGKTVYLSTTDSGKTWNCTPGDVDAKYLPGACR"
     gene            1451..3031
                     /locus_tag="AC158Q_08710"
     CDS             1451..3031
                     /locus_tag="AC158Q_08710"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3339897.1"
                     /translation="MLDWIKNALSLRFRRASAADRASRTRLDGWMHAAIGCRQAGDDT
                     QAQELCRRILDEAPDHADALYLLGEIAAEHSDHDEAIGLIRRSISIASDRPQFHYALG
                     CVLGASGDWRGAMESYRSALALSSSHAGAHLNLGCILQARGEIDAAEDFLRDHATREL
                     DEALMHFRAVADIAPGHPDGWINLGYAMARRGNLENALLYYERALAIDAGLAQAQFSR
                     SMVLLAQGQFSAGWDAYEWRWQASGFPKPEFRQPEWDGSAVDRKTVFLYTEQGFGDAI
                     QFARYATLVAARGAQVILRCPPELTRLLGTVPGVTRTLASQDPMPDFDFHCPLLSLPR
                     VLRTTVDTIPAAVPYLRAEPEKKDEWRKLLDDGASGIKIGLVWASQSQFPGAELKSMA
                     LESLGPLRRLAGATFYSIQMGAAGRQAATPGAPIAVHDLTGRIRDFTDTAALIDNLDL
                     TLSVDTAVAHLAGAMGKPVWTILPYAADWRWEPRAAPGKWYPTMRLYRQTNRGDWVSV
                     LSQVANDLQQHFHLRLLP"
     gene            3065..>4294
                     /locus_tag="AC158Q_08715"
     CDS             3065..>4294
                     /locus_tag="AC158Q_08715"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-linked N-acetylglucosamine transferase,
                     SPINDLY family protein"
                     /protein_id="HIR3339898.1"
                     /translation="MGILSRLFFGKRGTATVPDRETLAAWVRDGYDHQVRGNIEEAQR
                     LFHAVLEHDPRHPDVLFFLGASAANDRRELEAVHFLEKAVDARPEDGKFNFTLASVLH
                     EMGRFEEAVPYFRNAVRLQPDHPDATGMLWAALLETSYRGGQRREEELRVEVEKARDS
                     GKADIYSDILLAAIYRNQGRIDDSIAASRRLLQRVPDHSDTYSDLLLTMNYSEAYGPD
                     ELFAEHERYAARFARPYVAPPVDRTWPRRLRVGYVSPDFRSHVVALFFEPIVELHDRD
                     RFEVFCYYNHRRNDDFTARCRAAADHWLDCVHLSDAELADRIRADRIDILVDLAGHTG
                     FNRLKVFAMKPAPVQVTYLGYPNTTGLAAIDYRITDARVDPPGVADRMSAEKLVRMPD
                     GFHCFRPRPDSPEVGPLP"
BASE COUNT          815 a         1282 c         1339 g          858 t
ORIGIN      
        1 gggtttactg taacttgctg acttctggac agattagttc agtcattacc tggcccgttt
       61 cctgctaagg tgaaacccga atattcagca gcaagagtaa ttcagcagac ttgccgcgag
      121 ttttgacagg gggagtcatg gaacgcatgc aaaaagggtt tacgctgatc gagctgatga
      181 tcgtggtcgc gattatcggc attctcgccg ccgtggcgat tccggcctac caggactacc
      241 tggcgcggtc gcagatgtcc gaggcggtga atctgctcgg ttcgggcaag acgccgctcg
      301 ccgagtactt cgcggacaag gggacgtggc cggcggcact atccgacgtg atggggaata
      361 caacgggcaa gtacaccggg tccatcgtga tgacgggtac cccgacggtc agcggcacgg
      421 catcggccta tacgctggcc gcgagcatga agacgtccac catcaactcg gccattaccg
      481 gcaagacggt atatctgtcg actaccgatt ccggcagatc ctggaattgc gcagctggcg
      541 acgttgatgt cagatacctg ccgggcgcct gccggtaagc gaactgttcg tcgcctgacc
      601 cgcgcggcga tcctccaaga ctaacgctgg atactggagt tacgcgggcg ggcggggcgt
      661 ggcgccagcc acgcttacgc gtcatccatc ggcgccccgg gcgatatcgg cgagatcctt
      721 caaccctgcc gtattttgtc agtaagctga cacttttggg cgcctgagct gttttgggtt
      781 cgtgaaccag ttctcgtcgt tgcccagtat gatatataac ataatgatta ttaaagatat
      841 atttaggtgt actctaactg gcccgaatac tgctatctta gggtcaccga gcgagcagct
      901 tggttggttt gcccacctta catttttgaa gggagcattt ggatgaagag cttacagaag
      961 ggctttacgc tgatcgaact gatgatcgtg gtcgcgatca tcggcattct cgccgccgtg
     1021 gcgattccgg cctatcagga ctacatcgcc cggtcgcaaa tgtccgaagc ggtgaacctg
     1081 ctcggttcgg gcaagacgcc gcttgccgag tacttcgcgg acaaggggac ctggccaggc
     1141 gcactcgccg acgtgatggg aaatacctcg ggtaagtaca ccgggtctat cacaacgacg
     1201 ggcactccga cggtcaacgg aacggcctcg gcttatacgc tggccgcgag catgaagacc
     1261 tccaccatca actcagccat taccggcaag acggtatatc tttcgacgac cgattccggt
     1321 aagacctgga attgcacgcc gggcgacgtt gatgccaagt acctgccggg cgcctgccgc
     1381 taagacgggc cgcttcggag aaaagcccgc tcgcgcgggc tttttttttg gtctgcctgt
     1441 ctgatgctcc atgctggatt ggatcaagaa cgcactatcg cttcgtttta gacgcgcttc
     1501 cgccgcagac cgtgcaagcc ggacccgtct ggacggctgg atgcatgctg ccatcggctg
     1561 ccgccaggcc ggggacgata cacaggctca ggaactctgc cgccgcatcc tggatgaagc
     1621 cccggaccac gcggacgcgc tgtacctgct cggcgagatc gcggcagagc attcggatca
     1681 cgacgaagcg atcgggctga tccgccggtc aatatccatt gcgagcgacc ggccgcagtt
     1741 ccattacgcg ctcggctgcg tgctcggcgc gagtggggac tggcggggcg ccatggagag
     1801 ctatcgcagc gcgcttgccc tctcctcgtc acacgcgggc gcgcatctca atctcggctg
     1861 catcctccag gcccggggcg agatcgatgc cgcggaagat tttctgcgcg atcacgcgac
     1921 tcgggaactc gatgaggcac tcatgcactt cagggcggtg gcggacatcg cgcctggaca
     1981 tcccgatggc tggatcaatc tcggatacgc gatggctcgc cggggcaacc tggagaatgc
     2041 cctgctatat tacgagcgtg cgctggcgat cgacgccggt cttgcgcagg cgcaattcag
     2101 ccgttccatg gtgctgctcg cacaagggca gttttctgcc ggatgggatg cctacgaatg
     2161 gcgttggcag gccagcggat ttccgaaacc ggagttccgg cagccggaat gggatggctc
     2221 cgccgtcgac aggaagacag tttttctgta caccgagcag ggtttcgggg atgccattca
     2281 atttgcgcgc tatgcaacac tggtcgcggc ccggggcgca caggtgattc tccgttgccc
     2341 tcccgagctg acgcgattgc tcgggacggt accgggtgtg acgcgcaccc tggcctcaca
     2401 ggatcccatg cccgactttg attttcattg tccactgttg agcctgccgc gagtactccg
     2461 tacgacggta gatacgatcc ctgctgccgt gccctatctg agggccgagc ccgaaaagaa
     2521 agacgaatgg cgcaagttac tggacgatgg cgcgtcgggc atcaagatcg gtctcgtctg
     2581 ggccagccag tcccagtttc cgggcgcgga gctcaagtcg atggcgctgg agtcgctcgg
     2641 gccgctgcgc cggctcgcgg gcgccacgtt ttacagcatt cagatgggcg cggcaggcag
     2701 gcaggcggcg actcccggcg cgccaattgc agttcacgac ttgaccggtc gcatacggga
     2761 tttcaccgac accgctgccc tgatcgacaa tctggatctc accctgtcag tggatactgc
     2821 tgtcgcgcac cttgccggcg cgatgggcaa accggtgtgg acgattctgc cttatgctgc
     2881 cgactggcgg tgggagccgc gcgcggcgcc tggcaagtgg tatcccacga tgcggcttta
     2941 ccgtcagacg aatcggggag actgggtgag cgtattgtct caagttgcca acgacctgca
     3001 gcagcacttc cacctgagac tgcttccctg aaaaaagcga caaagcgctg gtccggatta
     3061 attcatgggt attctcagca ggctgttttt cggtaaacgc ggcaccgcga ccgtcccgga
     3121 tcgcgagacg ctcgccgcgt gggtgcgtga cggttacgat caccaggtcc gcggaaacat
     3181 cgaagaggcg cagcgtttgt ttcatgcggt gctggagcac gatccccggc accccgacgt
     3241 cttgttcttc ctcggcgcca gtgccgcgaa cgaccggcgc gagctggaag ccgtacattt
     3301 cctggaaaaa gcagtagatg cccgtcccga agacgggaaa ttcaacttta ccctggcgtc
     3361 ggtcttgcat gaaatgggcc gcttcgagga agcggtgccg tatttccgga acgcggtgcg
     3421 tttgcagccc gatcatcccg atgcgaccgg catgctttgg gcggccctgc tcgagacgag
     3481 ctaccgggga gggcagcgcc gcgaggagga attgcgcgtt gaggtcgaga aggcgagaga
     3541 ctcgggcaag gcggacatct acagcgacat actcctagcc gccatctacc ggaaccaggg
     3601 gcgcatagat gactccattg cagcttcgcg ccggctgctg caaagagtgc cggatcattc
     3661 cgatacctat agcgacctgc tgctgacgat gaattacagc gaggcgtacg gccccgacga
     3721 gctgttcgcc gagcacgagc gctacgcagc gcgctttgcg cggccgtacg tcgcgccacc
     3781 ggtggaccgg acctggccgc gcaggctgag ggtcggatac gtatcccccg attttcgctc
     3841 gcatgtcgta gccctgttct ttgaacccat cgttgagctt catgaccgcg atcgattcga
     3901 ggtgttctgc tactacaacc accggcggaa tgatgatttt actgcgcgct gccgggctgc
     3961 ggcggatcat tggctcgatt gcgtgcatct ttcggacgcc gaactcgcgg accgcatccg
     4021 ggccgaccgg atcgacatac tggtggacct tgccgggcac acgggcttca accggctcaa
     4081 ggtatttgcg atgaagccgg cgccggtgca ggtgacctat cttggctatc cgaacaccac
     4141 cggactggcc gcaatcgatt accgaatcac cgacgcgcgc gtcgatccgc ccggagttgc
     4201 ggatcgcatg agcgcggaaa agctcgtgcg catgccggac ggctttcatt gtttccggcc
     4261 gcgcccggac agcccggagg tcggaccgct gccg
//
LOCUS       DBNGSG010000334         1798 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3843834, whole genome shotgun sequence.
ACCESSION   DBNGSG010000334 DBNGSG010000000
VERSION     DBNGSG010000334.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3843834"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1798)
                     /locus_tag="AC158Q_08720"
     CDS             complement(<1..>1798)
                     /locus_tag="AC158Q_08720"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3339899.1"
                     /translation="LAKIESGTVTLSIEPERVSELGDYVERTFRQLAHDKRLEFDVTL
                     SPALPAFIHTDEKRLQQILKNLLSNAFKFTEQGRVSLHISRVKSGWTPGCPQLDAAGE
                     AVALSVADTGIGIPANKQKLIFEAFQQADGTTSRQYGGTGLGLSISRELARLLGGEIR
                     VVSAPGKGSTFTLYLPIDYQEAVSEDKTVAKKVAPESIPVPVGRDEPGSLLKMKPQLH
                     RQPAVRDDRDRIQAGDRVVLIIEDDVKFASALLDLARESGFKGVVALSANSALALVKE
                     VMPDAITLDLKLPDMDGWAVLDLLKHDPETRHIPVNVISVADHLHRSFYMGALGVVHK
                     SAAKEVLQESLARTRKFIEREIRRLLVASGDDAQRESIIGALVGDGLKVTAAGTGKEA
                     LEALQKDPFDCILVGPALPDMRPTELLRKFAKLRKAAEVPIVMFGADGLSRGDRDSLR
                     KLAEILVIKNVSTPEAVLEAATLFLHHAMADLPQQARDVLVLQRKSEKELSGKKALIV
                     DDDIRNIFALTSALEQHGMSVLNAENGKEGIEMLKNHSDVDIVLMDIMMPELDGYDTM
                     RVMRKLERFRDLPIIAITAKAMMGDREKCIEAG"
BASE COUNT          278 a          597 c          559 g          364 t
ORIGIN      
        1 ccggcctcga tgcacttttc gcggtctccc atcatcgctt tcgccgtgat ggcgatgatc
       61 ggaagatccc tgaaccgctc gagcttgcgc atgacgcgca tcgtgtcgta gccatcgagc
      121 tcgggcatca tgatgtccat gagcacgatg tccacgtcgg agtggttctt gagcatctcg
      181 atcccctcct tgccattctc cgcattgagc acgctcatgc cgtgctgttc cagcgcgctg
      241 gtcagcgcga agatgttgcg gatgtcgtcg tcgacgatca gcgccttttt cccgctcagc
      301 tccttctccg acttgcgctg gaggacgagg acgtcgcgcg cctgctgagg caggtcggcc
      361 atcgcgtggt gcaggaacag cgtagccgcc tccaaaacgg cttccggcgt gctgacgttt
      421 ttgatgacaa ggatctccgc gagtttgcgc aggctgtccc ggtcgccgcg gctcaagcca
      481 tccgcgccga acatcacgat gggcacttca gccgccttgc ggagcttcgc gaatttcctg
      541 agcagctccg tcgggcgcat gtcgggcagc gccgggccca ccaatatgca gtcgaacggg
      601 tccttctgca gggcttcgag cgcctccttc ccggtgccgg ccgcggtgac cttcaggcca
      661 tcgcccacca gcgcgccgat aatgctctct ctctgcgcat catcgccact ggcgacgagc
      721 agcctgcgga tttcacgctc gatgaacttg cgtgtccggg cgagcgactc ctgcaggacc
      781 tccttggccg cggatttgtg caccacgccc agagccccca tgtagaaaga gcggtgcagg
      841 tggtcggcca ccgaaatgac gttcaccggg atgtgccgcg tttcggggtc gtgtttcagc
      901 aggtccagta ccgcccagcc gtccatgtcc ggcagcttca ggtcgagcgt gatggcgtcc
      961 ggcatgactt ccttgacgag cgccagggcc gagttcgcac tcagggcgac cacccccttg
     1021 aacccgctct cgcgcgcgag atcgaggagc gcggaggcga acttgacgtc gtcttcgatg
     1081 atcagcacca cgcggtcacc ggcctggatc cggtcacggt cgtcgcgcac cgccggttgc
     1141 ctgtgcaatt gcggcttcat cttcaacaaa ctgccgggct cgtcccgccc gaccgggacg
     1201 gggatggatt ccggcgcgac ctttttcgcg accgttttgt cctcgcttac cgcttcctgg
     1261 tagtcgatcg ggaggtagag cgtgaacgtg ctgcccttgc cgggggcgct gacgacgcgg
     1321 atctccccgc cgagcaggcg cgccagttcc cggctgatgg atagccccag cccggtgccc
     1381 ccatactggc ggctggtggt cccgtcggcc tgctggaatg cctcgaaaat cagtttctgc
     1441 ttgttcgccg ggatgccgat gccggtgtcc gccacggaca acgccaccgc ctcgcccgcc
     1501 gcgtcgagtt gagggcaccc cggggtccag ccggatttca cccgcgagat atgcagcgac
     1561 accctgcctt gctcggtgaa cttgaacgcg ttggacaaaa ggttcttgag gatctgctgc
     1621 agccgcttct cgtcggtatg aatgaaggcg ggcagcgccg gcgacagggt cacgtcgaat
     1681 tccagccgct tgtcgtgcgc cagctggcgg aacgtccgtt cgacgtaatc gcccagctcg
     1741 gatacccgct cgggctcgat gctgagcgtg acggtgcccg attcgatctt ggcgagat
//
LOCUS       DBNGSG010000335         3451 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4523517, whole genome shotgun sequence.
ACCESSION   DBNGSG010000335 DBNGSG010000000
VERSION     DBNGSG010000335.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4523517"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="AC158Q_08725"
     CDS             complement(<1..470)
                     /locus_tag="AC158Q_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HIR3339900.1"
                     /translation="MAKIIGIDLGTTNSCVAVMENGQPKVIENSEGARTTPSIVAYME
                     DGEILTGAPAKRQAVTNPKNTLYAVKRLIGRRFADAEVQKALKVVPYQIVEASNKDAW
                     VEVRGKKMAPPEISAQVLMKMKKTAEDYLGEPVTEAVITVPAYFNDSQRQATKDA"
     gene            complement(529..1080)
                     /gene="grpE"
                     /locus_tag="AC158Q_08730"
     CDS             complement(529..1080)
                     /gene="grpE"
                     /locus_tag="AC158Q_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIR3339901.1"
                     /translation="MSEDDDAARIEPVLTELPAPRMPADAPAGTANTVEELRKAELAA
                     AEHREAWLRAKAEADNVRKRAQVEITNAHKFAVENFSTELLAVKDSLEAALAAENATA
                     ESMKSGVELTLRQLKNVFEKFNVSEVNPAGQKFDPHRHQAIGTVESSAEPNTVVQVLQ
                     KGYLLHDRVIRPALVMVAKSVES"
     gene            complement(1248..1598)
                     /locus_tag="AC158Q_08735"
     CDS             complement(1248..1598)
                     /locus_tag="AC158Q_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018004683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HIR3339902.1"
                     /translation="MKLLLVWIINALSLLALPYVFTSIRVDSFTTAMIVALVLGLVNA
                     VIRPLLIVLTFPVTIVTLGLFIFVINGLLFGFVASFIEGFAVAGFWPAVLGAIVYSVI
                     SWLCSAIVLGGKKD"
     gene            complement(1633..2727)
                     /gene="hemH"
                     /locus_tag="AC158Q_08740"
     CDS             complement(1633..2727)
                     /gene="hemH"
                     /locus_tag="AC158Q_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - protoporphyrin ferrochelatase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HIR3339903.1"
                     /translation="MPHYTPEPPFSHGAPSRIGVLLINLGTPEAPTATAVRAYLKEFL
                     SDPRVVEIPRILWWPVLNLFVLTTRPKASAQRYAQVWMSEGSPLRVHTERQTTLLRGY
                     LGERAKSLPLAVDYAMRYGRPSIPDKLLEMKSQRCDRILLVPLYPQYSASATATAFDA
                     AFRCIEGMRNQPALRTVRSFHDHPGYIAALAQNVRDFWMKNQRPDVLVMSFHGVPRSA
                     LDKGDPYHCECHKTGRLLAEALGLKQEQYRITFQSRFGRAEWLKPYTADMLVELARQK
                     AGRVDVVCPGFVSDCLETLEEIAIEGKMIYLNAGGRDFHAIPCLNERNDWAHALADIV
                     TANLVGWGDSATHETLELGRLRALSMGAKS"
     gene            complement(2829..>3451)
                     /locus_tag="AC158Q_08745"
     CDS             complement(2829..>3451)
                     /locus_tag="AC158Q_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HrcA family transcriptional regulator"
                     /protein_id="HIR3339904.1"
                     /translation="HIEFLRLSDTRVLLILVTPEGDVQNRILSAEKSYTPSELTEAAN
                     ILNQNYAGLNFDEIRRRIREELKQLTEDMTRLMSAALEAGSQAASESAEDMVISGESN
                     LLDSQDLSSNMVNLRKLFDLFDRKTGLMQLLDISSRAQGVQIFIGGESGVAPLDECSV
                     ITAPYTVEGQVLGTVGVIGPTRMAYERVIPIVDITAKLLSSLLTQR"
BASE COUNT          573 a         1063 c         1119 g          696 t
ORIGIN      
        1 gcgtccttgg ttgcctggcg ctgcgagtcg ttgaaatacg cgggcaccgt gatcaccgct
       61 tcggtgaccg gctcgccgag gtagtcctcg gcggtcttct tcatcttcat cagcacctgc
      121 gcggagattt cgggcggcgc catcttcttg ccgcgcacct cgacccaggc gtccttgttg
      181 gacgcctcca cgatctggta cggcactacc ttgagcgcct tctgcacttc ggcgtcggcg
      241 aagcggcggc cgatcaggcg cttgaccgcg tagagagtgt tcttgggatt ggtgactgcc
      301 tggcgcttgg ccggggcgcc ggtgagtatt tccccgtcct ccatgtaggc gacgatggag
      361 ggagtggtgc gggcgccctc ggagttctcg atcaccttgg gctggccgtt ttccatgacg
      421 gctacgcacg agttggtcgt tcccaggtca atgccgatga tcttagccat gttctacctc
      481 gttgctttca aacaaaagtt gtccagttac agatatgggg cccaactctc agctttcaac
      541 ggacttcgcg accatcacca gggccggacg gatcacccgg tcgtggagca ggtagccctt
      601 ctgcagcacc tgtaccacgg tattgggctc ggcgctggat tccaccgtgc cgatcgcctg
      661 gtggcggtgg gggtcgaatt tctggcccgc cgggtttacc tcggagacat tgaatttttc
      721 gaacacgttc ttcaactgcc tgagggtgag ttcgacgccg cttttcatgc tctccgccgt
      781 cgcgttttcc gcggccagcg cggcttcgag actgtctttc acagcgagca gttccgtgga
      841 gaagttctca accgcgaact tgtgggcgtt ggtgatctcg acctgcgcgc gcttgcggac
      901 gttgtccgct tccgctttgg cgcgcagcca tgcctcgcga tgttccgcgg ctgccagttc
      961 cgctttcctc agttcctcga cggtatttgc cgtgccggca ggggcatcgg ccggcatccg
     1021 cggcgcgggc aattccgtca gcacgggttc gatccgggcc gcgtcatcat cttcggacat
     1081 cttatccacg ctgtcttccg ttaactcatt gaatattggg cttgaccggg gtaattcaag
     1141 gggcgtgttt ataccatcaa gtaattgttt tataagttgt ttttcgtcgc ggctgccggc
     1201 ctgcccgttg cagggccctt ggcccgggcg acggtcgcag cggccggcta gtctttcttg
     1261 ccgcccagaa cgatggcgct gcagagccag ctgataacgc tatagacgat cgcgcccaat
     1321 acggccggcc agaagccggc gacggcgaac ccctcgatga aggacgcgac gaacccgaac
     1381 agcagcccgt tgatgacgaa gatgaacagt cccagcgtca cgatcgtcac gggaaacgtg
     1441 agcacgatca gcagcgggcg aatgacggcg ttaaccagcc ccagcacgag cgcgacgatc
     1501 atcgcggtgg tgaagctgtc gacccggatg gaggtgaaca catagggcag ggcgagcagc
     1561 gacaacgcgt tgatgatcca gacgagaagc aatttcacgg ttttcctcct ggcttgaatc
     1621 cgggcgcccg cttcaactct tcgcgcccat ggacaaggcg cgcaggcgcc ccagttcgag
     1681 cgtttcgtgc gtcgcggagt cgccccagcc aacgagattg gccgtgacga tgtcggctag
     1741 cgcgtgggcc cagtcgttgc gctcgttcag gcacgggatg gcgtggaaat cgcgcccgcc
     1801 cgcgttgagg taaatcatct tgccttcgat cgcgatctct tcgagcgtct cgaggcagtc
     1861 ggacacgaag ccggggcaga ccacgtcgac gcgcccggct ttctgccggg cgagctcgac
     1921 cagcatgtcc gcggtatagg gcttcaacca ctccgcgcgg ccgaagcgcg attggaacgt
     1981 gatgcggtac tgctcctgtt tcagtcccag cgcttccgcg agcagccgcc cggtcttgtg
     2041 gcactcgcaa tggtaggggt cgcccttgtc cagcgccgag cgcggcacgc cgtgaaagct
     2101 catcaccagc acgtccggcc gctggttctt catccagaag tcgcgcacgt tctgcgcgag
     2161 cgcggcgatg taacccgggt ggtcgtggaa gctgcggacc gtgcgtagcg ccggctggtt
     2221 gcgcatgccc tcgatacacc ggaatgccgc gtcgaaagcc gtcgccgtgg cactggccga
     2281 gtactggggg tagagcggta ccagcaggat gcggtcgcag cgctgcgatt tcatctccag
     2341 cagcttgtcc gggatcgagg gcctgccgta gcgcatggcg taatccacgg cgagcggcaa
     2401 cgatttggcg cgctcgccga ggtagccgcg cagcagcgtc gtctggcgct cggtgtgcac
     2461 ccgcaacggc gagccctcgc tcatccacac ctgggcgtag cgctgggcgg aagccttggg
     2521 gcgcgtggtg agcacgaaaa gattcaggac cggccaccag agaatgcgcg ggatctcgac
     2581 cacccgcggg tccgacagga attccttgag ataggcgcgc actgccgttg ctgtcggcgc
     2641 ctccggcgtg ccgagattga tcagcagcac cccgatgcgg gagggggcgc catgagaaaa
     2701 cggaggctcg ggcgtgtagt ggggcatgag ctatgcccca agtgatgagt gatgagtaat
     2761 gagtgatgag caggaaaccg ctgtccgcag cgcccctgtt ccgtcactcg tcactcgtca
     2821 cccttcactc aacgttgtgt gagcaggctg gagagcagct tggccgtgat gtccacgatg
     2881 gggatgacgc gctcgtaagc catgcgggtg gggccgatca cgccgacggt gccgagcacc
     2941 tggccttcca cggtgtaggg ggccgtgatc acgctgcact cgtcgagcgg tgccaccccc
     3001 gactcgccgc cgatgaagat ttgcacgccc tgcgcgcggc tcgaaatgtc gagcagctgc
     3061 atcagcccgg tcttgcggtc gaaaagatcg aagagcttcc gcaggttcac catgttggag
     3121 gagaggtcct gcgagtcgag cagattgctc tcgcccgaga tcaccatgtc ctccgcggac
     3181 tcgctcgcgg cctggctgcc cgcctccagg gccgcgctca tgagccgcgt catgtcttcg
     3241 gtgagctgct tcagttcctc gcggatgcgc cgccggatct cgtcgaagtt caacccggcg
     3301 tagttctggt tgaggatgtt cgccgcctcg gtgagctcgg agggcgtgta cgatttctcg
     3361 gcgctgagga tgcggttttg cacgtcgccc tcgggcgtga ccaggatcag caggacgcgc
     3421 gtgtccgaaa gcctgaggaa ctcgatgtgc c
//
LOCUS       DBNGSG010000336         9418 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_279440, whole genome shotgun sequence.
ACCESSION   DBNGSG010000336 DBNGSG010000000
VERSION     DBNGSG010000336.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9418)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9418)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9418
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_279440"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..1565)
                     /gene="purH"
                     /locus_tag="AC158Q_08750"
     CDS             complement(3..1565)
                     /gene="purH"
                     /locus_tag="AC158Q_08750"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HIR3339905.1"
                     /translation="MAVIKQALISVSDKTGVVEFARGLAGFDVALISTGGTARLLRDA
                     GLKVTEVADHTGFPEMLDGRVKTLHPRVHGGILARRDVPGHLAELAQAGIPAIDLVVV
                     NLYPFAAAVAKPGATLAEAIENIDIGGPAMVRSAAKNYEHVAVVTDPADYQPVLKEMA
                     AAAGALGAAMRFHLAQKAFSHTAQYDGAISNYLTSLDSAGGRARFPQRLNLQFERVQS
                     MRYGENPHQEAAFYRDPRPAPGSLGAYRQLQGKELSYNNIADADAAWECVKSFAEAAC
                     VIVKHANPCGAAVAGDLAEAYRKAFATDPTSAFGGIIAFNRPLDGPTAQAVTQQFVEV
                     VIAPGIAPEAARVLAGKSNVRVLEVTLAPGANDYDFKRVGGGLLVQTPDSASIDAGGL
                     KIVTRVKPTPAQITDLLFAWRVARFVKSNAIVFCGGGRTLGVGAGQMSRVDSVRIAAI
                     KAENAGLPLAGSVVASDAFFPFRDGVDAVARSGASAVIQPGGSLRDEEVIAAANEHGI
                     AMVFTGYRHFRH"
     gene            complement(1661..1897)
                     /locus_tag="AC158Q_08755"
     CDS             complement(1661..1897)
                     /locus_tag="AC158Q_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3339906.1"
                     /translation="MNENEMARMVRKAIDGYFRDLDGEKPCAVYDMVIHCVEKPLLES
                     VLHRVRGNQSHAAAMLGINRNTLRKKMRVHGIKF"
     gene            complement(1897..2904)
                     /gene="dusB"
                     /locus_tag="AC158Q_08760"
     CDS             complement(1897..2904)
                     /gene="dusB"
                     /locus_tag="AC158Q_08760"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HIR3339907.1"
                     /translation="MHIGPYQLKNNLIVAPMAGVTDRPFRQLCKTMGAGMAVSEMVAS
                     NSLLWGSEKTRRRANHEGEVDPISVQIAGADPRMLADAARYNVDQGAQIIDINMGCPA
                     KKVCNVMAGSALLKDEPLVGRILDAVVGAVNAPVTLKIRTGWDRGNRNAILIARTAQQ
                     AGIRALAIHGRTRACGFSGDAEYDTIAAVKAEVGIPVIANGDISTPEQVKEVLDYTRA
                     DAVMIGRAAQGRPWMFREILHYLATGKHLPPPEVEEIHRVLVAHLNDLYEFYGEYTGV
                     RVARKHISWYTRGLAGSAAFRHAMNRLETRLEQLDAVNGFFTQLASRGQRLTYVEELA
                     A"
     gene            complement(3137..4285)
                     /locus_tag="AC158Q_08765"
     CDS             complement(3137..4285)
                     /locus_tag="AC158Q_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR3339908.1"
                     /translation="MPDYDVLIVGGGPVGAAAALALRGTGLSLGLVEARAPQAAHADP
                     RPIALSHGSRLILERLGMWDALAPATPIERIHVSQRGGFGRAQLDAAEVGLPALGYVL
                     DYGRLASTLAGALEEHDACHLVRATVSALKAGPESALARFEAGPGSGQATARLLIVAE
                     GGTLALARMRTRDYGQSVVTARVESEAPHRNVAYERFTPDGPLALLPMGERLALVWTT
                     APQRALQLCELPEASFLERLQQAFGSRLGAFSRASARSRYPLVLKVADRIAEQRVVAL
                     GNAAQTLHPVAGQGLNLGLRDAWELAETVRSRDKSEIGGSSHLKAYQARRRLDRRGGI
                     GMTDALVRLFSNDIAPLRLARGLGLAALGAVPPARNFLVRRMTFGSRG"
     gene            complement(4278..5597)
                     /locus_tag="AC158Q_08770"
                     /pseudo
     CDS             complement(4278..5597)
                     /locus_tag="AC158Q_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194077.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
     gene            complement(5643..6338)
                     /gene="murU"
                     /locus_tag="AC158Q_08775"
     CDS             complement(5643..6338)
                     /gene="murU"
                     /locus_tag="AC158Q_08775"
                     /EC_number="2.7.7.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008168640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramate alpha-1-phosphate
                     uridylyltransferase MurU"
                     /protein_id="HIR3339909.1"
                     /translation="MKAMILAAGRGERMRPLTDRVPKALLPVGGRPLIAHLIGRLALA
                     GFTELVLNVSHLAGLIERELGDGSNLGVHIVYSREQQALETGGGIAYALPLLGAEPFV
                     VVNSDIYCDFDFARLASAEMSLASGVLSAHLVLVDNPPHHPAGDFRLREGKVTAHGDE
                     PRLTFSGIGVYAPALFTEVARGTRRQLAALLTPEMARGRVTGEHHRGRWMDVGTPQRL
                     ADLDRLLAPARRA"
     gene            complement(6358..7404)
                     /locus_tag="AC158Q_08780"
     CDS             complement(6358..7404)
                     /locus_tag="AC158Q_08780"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HIR3339910.1"
                     /translation="MLKSHNSWSKAIGLQVSSDIRSEQLRQWLTAHVAASAFTMQPAS
                     ADASFRRYYRVAADGRTWIAMDAPPPHEDCRPFVRVAGLLRAAGVNAPRVEAQDLERG
                     FLLLTDFGDTTYLAALDETNADRLFGYAIEALLKWQLASREGELPAYDGALLQRECNL
                     FPEWYVARHLGAALSDAQQGAFSAMLRLLIARACAQPAVYVHRDYMPRNLMVTEPNPG
                     VIDFQDAVYGPISYDVVSLTRDAFVSWDEERVIDWAARYWEKAKNAGLPVDPDFSRFY
                     QDFEWMGLQRHLKVLGIFARIHYRDGKPGYLEDTPRFMRYARAVAERYRELAPLARLF
                     DQIEKRQPAAGRAS"
     gene            7427..>9418
                     /locus_tag="AC158Q_08785"
     CDS             7427..>9418
                     /locus_tag="AC158Q_08785"
                     /inference="COORDINATES: protein motif:HMM:NF016348.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HIR3339911.1"
                     /translation="MRRLEISRISFAVLCALPLAAGAQQGLKLKPQPTLTLLPPGTRE
                     DVPLFLDADRLQGHNDRETEATGSVRLRQRGQAVHADRLRYDKPTDEVTAEGSVRIER
                     GADVVEGVLLQYNLGTGRGFMDKPSYMLHKSPDLGGQRQLFRDTDARGTAERIFFEGP
                     GQYRAQGASYTACAPGNDDWYLRAGDLAIDKGRDVGVARNASIVFMGTPIFYSPYLSF
                     SLHQERKSGFITPHYGSTNKGGAELTVPYYWNIAPNRDATIAPRVMTRRGVLFNNEFR
                     YLEPNYLGEARADLLPEDNAHGGERRWAYNIRHNHTLPSGWFGTLNLQKVSDDTYFTD
                     LSTQIAATSQVLLPREGVLGRNGSWGSTGHYGVGALVQRWQTLQADPLALVTPPYNRL
                     PQLTLNAGRQDVLRTDFDFHGHYVGFDHPTQVNGKRALAYPSLSLPLATAFAQVTPKL
                     GLNVTRYAIDPNTAGFEGQTRTLPIFTTDSSVVLERPAVIGGTSFLQTLEPRLYYVYI
                     PYKDQSRIPVFDSGQQDINLSTIYSENQYAGWDRINDANQVTLGVTSRLISADSGAEL
                     LSGGVAQRYYFETQRVTLPGVPARTSTSSDLLAVISGAVAPHWTADAGWQYSTNFSQT
                     QKFNIGTRYQPQPGKVLNLSYRETLNVLRQTDISAQWPVG"
BASE COUNT         1602 a         3220 c         3024 g         1572 t
ORIGIN      
        1 cgtcagtgcc ggaaatgccg gtagccggtg aagaccattg cgatgccgtg ctcgttggcg
       61 gcggcgatca cttcctcgtc gcgcagcgag ccgccgggct ggatcaccgc ggaggcgccg
      121 ctgcgggcca ccgcgtccac gccgtcgcgg aacggaaaaa aggcgtcgga ggcgaccacg
      181 gaccccgcga gcggaagtcc cgcgttctcc gccttgatgg cggcgatgcg caccgagtcc
      241 acacggctca tctgtcccgc gcctaccccc agcgtccggc ccccgccgca gaatactatt
      301 gcattcgact tgacgaacct ggccacgcgc caggcgaaca acagatcggt tatttgcgcc
      361 ggggtcggct tcacccgcgt gacgatcttg agtcccccgg catcgatgct cgcgctatcg
      421 ggcgtctgca ccagcagccc tccgcccacg cgcttgaagt cgtaatcgtt ggcgcctggc
      481 gcaagcgtca cctcgagcac gcgcacgtta ctctttcccg ccagcacgcg ggccgcctcg
      541 ggtgcgatgc cgggagcgat caccacttcg acgaactgct gtgtgaccgc ctgcgccgtg
      601 ggcccgtcga gcgggcggtt gaaagcaatg atcccgccga aagccgaggt ggggtcggtc
      661 gcgaaagcct tgcggtaggc ttcggcaaga tcgcctgcaa cggcggcacc gcaggggttg
      721 gcatgcttga cgatgacgca cgccgcctcg gcgaagctct tcacgcactc ccaggccgcg
      781 tcggcatcgg caatgttgtt ataggacaat tccttgccct gcaactggcg ataggcgccg
      841 aggctgccgg gggccggccg gggatcgcgg tagaaggcgg cttcctggtg cgggttctcc
      901 ccatagcgca ttgactgaac ccgctcaaac tggaggttca accgctgagg gaagcgggcg
      961 cggccacccg cggagtcgag cgaagtcagg taattgctga tcgcgccatc gtattgggcg
     1021 gtatgggaga acgccttttg cgcaaggtga aagcgcattg cggccccgag tgcgccggcg
     1081 gctgcagcca tctccttcag caccggctgg taatccgccg ggtcggtcac cactgccacg
     1141 tgctcgtagt tcttggcggc gctcctcacc atcgccgggc cgccgatatc gatgttctcg
     1201 atcgcctcgg cgagcgtagc gccgggcttg gccacggccg cggcgaaggg atacaggttg
     1261 acgaccacca ggtcaatcgc cggaatgcct gcttgcgcga gctcggccag gtggccgggg
     1321 acatcgcgcc gggctagaat gccgccgtgc actcgcggat gcagggtttt gacccgaccg
     1381 tcgagcatct ccggaaaacc ggtatggtcg gcgacctcgg tgaccttgag gccggcgtcc
     1441 cggagcagcc tggcggtgcc gccggtggaa atcagcgcga catcgaagcc ggccaggccg
     1501 cgcgcgaatt cgacgacgcc ggtcttgtcc gaaacgctga tcagcgcctg cttgatgacg
     1561 gccatggggc gagtgagcgg taagcagtca gcggtaagca gtcaggtccc gcaaacggca
     1621 attctaaccg ctcaccgctt actgctcacg gcttacgccg tcaaaacttg atgccgtgca
     1681 ccctcatttt cttgcgcaag gtgttgcgat tgatgccgag catcgcggcg gcatgggact
     1741 ggttgccgcg cacgcggtgc agcacgctct ccagcagcgg cttctccacg cagtggatga
     1801 ccatgtcata gaccgcgcag ggtttctccc cgtccaggtc gcggaaatag ccatcgatcg
     1861 ccttgcgcac catgcgcgcc atttcgttct cgttgatcat gctgcgagtt cctcgacata
     1921 ggtcaggcgc tgcccgcggc tggcgagctg cgtgaagaag ccgttcaccg catccagctg
     1981 ctcgaggcgg gtctcgagcc ggttcatggc gtggcgaaac gccgccgatc cggccaggcc
     2041 cctggtgtac caggaaatat gcttgcgcgc gacacgtacg ccggtgtatt cgccgtagaa
     2101 ttcgtagaga tcattgaggt gggcgaccag cacgcggtgg atctcctcca cctccggtgg
     2161 cggaaggtgc tttcccgtcg cgagatagtg caggatctcg cggaacatcc atggccggcc
     2221 ctgggcggcg cgcccgatca tcacggcgtc cgcccgcgtg taatccagta cttccttgac
     2281 ctgctccggg gtggaaatgt cgccattggc gatgaccggg atgccgacct cggccttcac
     2341 cgccgcaata gtgtcgtatt ccgcatcgcc cgagaagccg caggcccggg tgcggccgtg
     2401 gatggcgagc gcccggatgc cggcctgctg agcggtccgg gcaatcagga tggcgttgcg
     2461 gttgccgcgg tcccagccgg tgcggatctt cagcgtgacc ggggcgttga ccgccccgac
     2521 caccgcatcg aggatgcgcc cgaccagggg ttcgtccttc agcagcgccg agcccgccat
     2581 cacgttgcac accttcttgg cggggcagcc catgttgatg tcgatgatct gggcgccctg
     2641 atcgacgttg tagcgcgccg catccgccag catccggggg tcggcgccag cgatctgcac
     2701 cgagatcggg tcgacttcgc cttcgtgatt ggcacggcgg cgcgtttttt ccgagcccca
     2761 caagagcgaa ttgctcgcca ccatctcgga caccgccatg cccgctccca tggtcttgca
     2821 cagctgccgg aacggacggt cggtgacccc cgccatcggc gcgacgatga gattgttctt
     2881 gagctgatac gggccgatgt gcatcgagcg tgccgcgtcc gcgatcgagt gcgtttgctt
     2941 acggggaagg gcgcattgta atcgctgcga ccgcgcaaga aaagcaatta ctgcggtttt
     3001 cctgatcggt gttgcggcca ccccacaagc cggccacgcc gcgatctcgt cacatcatca
     3061 aactgcttgg cccgctaagc acttactcaa aattttcgag cagtgcttgc gcgacggcac
     3121 gtaactgccg atgtgatcag ccgcgcgagc cgaaggtcat gcgccgcacc aggaagttcc
     3181 tggccggcgg cacggcgccg agcgccgcga gtcccaggcc gcgggcgagc ctgagaggcg
     3241 cgatatcgtt tgaaaacaat cgcaccagcg catcggtcat cccgatgccg ccgcggcggt
     3301 caaggcgccg ccgcgcctga taggccttga gatgcgagga gccgccaatc tcgcttttat
     3361 cgcggcttcg caccgtttcc gccagttccc aggcgtcgcg caatccaagg ttaagtccct
     3421 gcccggccac cggatgcagg gtttgcgccg cgttgcccag cgcaaccact cgctgttccg
     3481 caatgcggtc tgcgactttc aagacgagcg gataacggga gcgcgcgctg gcccggctga
     3541 aggcgcccaa tcggcttccg aaagcctgct gcagccgctc caggaagctg gcctcgggca
     3601 gttcgcacaa ttgcagtgcc cgctgcggcg cagtcgtcca caccagcgcg agccgctcgc
     3661 ccatgggcag caacgcgagc ggcccatccg gcgtgaagcg ctcataggcg acattccggt
     3721 gcggggcttc gctttcgacg cgcgcggtga ccacggactg gccgtaatcg cgagtacgca
     3781 tgcgggccag ggcgagcgtg ccgccttcgg ccacgatgag caggcgggcc gtggcctgcc
     3841 cgcttccggg gccggcttcg aaccgcgcga gggcggactc cggtccggct ttaagcgcac
     3901 ttaccgtggc acgcaccaga tggcaagcgt cgtgctcctc gagcgcccct gccaaggtcg
     3961 aggcgagccg gccgtaatcg agcacatagc ccagcgccgg caggccaacc tctgcagcat
     4021 ccagttgagc gcgtccgaaa ccgccccgct gcgacacgtg gatacgctcg atcggggtgg
     4081 ccggggcgag cgcgtcccac atcccgagcc gctccagtat caaccggctg ccgtgcgaga
     4141 gcgcgatcgg gcgcgggtcc gcgtgcgccg cctgcggcgc gcgcgcttcc accagcccca
     4201 gcgagagacc ggtaccgcgc agcgccagtg ccgccgcggc gccgaccggg ccgccgccca
     4261 cgatcaggac gtcgtaatca ggcatcgcgc gcgctcatcc aggcttcgat gtcggcgggc
     4321 cgcttcggtg catcacgggt gagcacctcg gcgccggcat cggtcaccgc cacgtcgtcc
     4381 tcgatgcgca ccccgatgcc cgcaaagcgt gccggcactc cctcaccggc cggtatatag
     4441 cagcccggct cgaccgtgag caccatgcct ggagcgagcg agcgccattc gccgccgcgc
     4501 ttgtagtcgc ccgcgtcgtg cacgtcgagc ccgagccagt ggccggtgcg gtgcatgtag
     4561 aatttcttgt aggcttcggt ctccacggcc tcggcgacgc cgccctggag cagtttcagg
     4621 tcgatcatcc cctgcgccag cacttcgacc gccgctcggt gcggcgcgtc ccagcggcct
     4681 ccgggcttca cttgtgcgat ggccgccgcc tgcgcggcca gcaccagctc gtagatctcg
     4741 cgttgcggcc cgctgaactt gccgttgacc ggaaaggtgc gcgtgacatc cgctgcgtag
     4801 ccgtccaact cgcaaccggc atcgatcagc agcagttcgc cgtccttcaa ctcgccgtcg
     4861 ttctgcacgt aatgcagcac gcaggcgcgc gcacccgatg cgacgatcgg ggtgtaggcc
     4921 ggcgcttgtg ccccattgcg ccggaactcg tgcatcagct ccgcctcgat ctcgtattcc
     4981 acgcggccag gccgcgctgc gcgcatggcg cgccggtgcg catccgcggt gatggccgcg
     5041 gcgcggcgca tcacggcgag ctcttccggc tccttgatga gccgcatctc gtcgagcggc
     5101 aaatgcacat ccctgatctc gcccggcgca gcgacgccgc tcctcgcgcg gccgcgcacc
     5161 tcattgatcc agcctgtcac gcgcgcatcc cacgacgcgt ccgcgccgag gtggcaatga
     5221 agcgcgccgc ggtctgcgac gagatccggc atcagtgcgt cgatctggtc gatcggatgc
     5281 gcctcgtcaa caccgaaagc cgcgcgtgcc gccgccgggc cgtaacggaa accgtcccag
     5341 gtctagcgct cgggatcctt gtcgcggcag aacaaaatat gtctcggttg tgcgcccgcg
     5401 acgaccacca gcaccgcctc gggctcacgg aacccggtga ggtagtagaa atagctgtcg
     5461 aaacgatacg gatagtgcga gtcgcgattg cggttgcgct cgggcgcggt cggcacgatc
     5521 gctacgcccg ctcccatcga gcgagcgagc cgctcccgcc gccgccggta aatgccggca
     5581 tccaggcctg ctgtcattct ggaattataa acaagcccgc gctcctgccc gcgcccgtcg
     5641 cgtcacgcgc ggcgcgccgg cgcaagcagc cgatcgagat cggccagtcg ctgcggcgtg
     5701 cctacgtcca tccaccggcc tcggtgatgc tcgccggtga cgcgtccgcg cgccatctcc
     5761 ggcgtgagca gcgcagcgag ctggcgccgg gttccgcgcg cgacctcggt aaacagcgcg
     5821 ggcgcataaa ccccgattcc gctgaaggtg agccggggtt cgtctccgtg cgccgtcacc
     5881 ttgccctcgc gcagccggaa atcgcccgcc ggatgatggg gcgggttgtc gaccaatacc
     5941 agatgggctg acaggacgcc gctcgcgagc gacatctccg cggaggcgag tcgcgcgaag
     6001 tcgaaatcgc agtagatatc gctgttgacc accacgaacg gctccgctcc cagcagcggc
     6061 agcgcgtagg cgatgccccc gccggtctcg agcgcctgct gttcgcgcga ataaacgatg
     6121 tggacgccga gattcgagcc gtcgcccagt tcgcgctcga tcagcccggc gaggtgcgag
     6181 acattgagca ccagctcggt aaagccggca agtgcaagtc ggccgatcaa atgcgcgatc
     6241 aacggccgcc cgccgacagg aagcaacgcc ttgggcacgc ggtcggtcaa cgggcgcatg
     6301 cgctctcccc gcccggcggc cagtatcatc gctttcacgt tcgatcgcgc ccgtcggtca
     6361 agaggctcgc cccgccgccg gctgccgctt ctcgatctgg tcgaagaggc gcgccagggg
     6421 cgccagttcg cggtaacgct cggcaacggc gcgcgcgtag cgcatgaaac gcggcgtgtc
     6481 ttcgagatag cccggtttcc cgtcgcgata atggatgcgc gcgaagatgc cgagcacctt
     6541 gagatgccgc tgcaggccca tccactcgaa atcctggtag aacctgctga aatccggatc
     6601 gaccggcagc cccgcgtttt tcgctttttc ccagtagcgc gcggcccagt cgatgacgcg
     6661 ctcctcgtcc cagctcacaa atgcgtcccg cgtgagcgat accacgtcgt agctgatcgg
     6721 cccgtacacc gcgtcctgaa aatcgatcac gccagggttg ggctcagtca ccatgaggtt
     6781 gcgcggcatg taatcgcggt gcacatagac cgcgggctgc gcgcacgcgc gcgcaatgag
     6841 gagcctgagc atcgcggaga acgccccctg ctgcgcgtcg gacagcgccg cgccaagatg
     6901 ccgcgcaacg taccactccg gaaacaggtt gcattcgcgc tgaagcaagg ctccgtcgta
     6961 ggcgggcagt tcgccttccc ggctggcgag ctgccatttg agcagcgcct cgatcgcgta
     7021 gccgaagagg cggtccgcgt tggtttcgtc gagcgcggca agataggtcg tgtcgccgaa
     7081 atcggtgagc agcaggaacc cgcgctcgag gtcctgcgcc tcgacccgcg gcgcgttgac
     7141 acccgccgcg cgcagcaacc ccgccacgcg cacgaacgga cggcagtctt cgtgcggcgg
     7201 cggcgcgtcc atcgcaatcc aggtgcggcc gtccgccgcc acgcggtaat agcggcggaa
     7261 gctggcgtcg gcggaagctg gctgcatcgt gaacgcgctc gccgcaacat gcgcggtcag
     7321 ccactgccgc agctgttccg atcgaatatc cgatgacacc tgcaatccga ttgccttcga
     7381 ccatgaattg tgcgatttta gcatctcact tccgctgtct ttcccaatgc gcaggctgga
     7441 aatatcacgc atatcgttcg ccgttttgtg cgcgctgccg ctggcggccg gcgcgcagca
     7501 gggattgaag ctgaaaccgc agcccacgct gaccctgctc ccgcccggga cccgggagga
     7561 cgtgccgctg ttcctcgacg cggaccggct gcaaggacac aacgatcgtg aaaccgaggc
     7621 cacgggcagc gtgcggctgc gccagcgcgg acaggcggtt cacgccgacc ggctgcgcta
     7681 cgacaagccc acggacgagg tcacggcgga gggcagcgtg cgcatcgagc gcggggctga
     7741 cgtggtggaa ggcgtgctgc tccagtacaa cctcggaacc gggcgcggct tcatggataa
     7801 gccgagctac atgctgcaca agagcccgga cctgggcggg cagcggcaat tgtttcgcga
     7861 cacggacgcg cgcggcaccg ccgagcgcat ttttttcgaa gggcccggcc agtaccgggc
     7921 gcagggcgca tcgtacaccg cgtgcgcgcc cggcaacgac gactggtacc tgcgggcggg
     7981 cgatctcgcc atcgacaagg gccgcgacgt gggcgtggcg cgcaatgcca gcatcgtgtt
     8041 catgggcacg ccgatctttt attcgccgta cctgtcgttc tcgctgcacc aggagcgcaa
     8101 gtccggcttc atcaccccgc actatggcag caccaacaag ggcggcgcgg aactgaccgt
     8161 cccttactac tggaacatcg cgcccaaccg tgatgccacc atcgcgccgc gcgtgatgac
     8221 gcggcgcggc gtgctgttca ataacgaatt tcgctacctc gaaccgaatt acctcgggga
     8281 ggcgcgcgcc gatctgctgc cggaggacaa cgcgcatggc ggagagcggc gctgggccta
     8341 caacattcgc cataaccata ccctgccgag cggctggttc ggcacgctca acctgcagaa
     8401 agtttcagat gacacctact ttaccgatct atccacccag attgccgcaa cctcacaggt
     8461 gctgttgccg cgcgagggcg tactcggtcg caacgggtcg tggggcagca ccggccatta
     8521 tggcgtcggt gcgctggtcc agcgctggca aacgttgcag gccgacccgc tggcgctggt
     8581 gactccgcct tacaaccgcc tgccgcagct gacattgaac gcgggacgcc aggacgtttt
     8641 gcgcacggac tttgattttc atggccacta cgtcggtttc gaccatccaa cgcaggtgaa
     8701 cggcaagcgc gcgctcgcct atccgagctt gagcctgccg ctcgccacgg cctttgccca
     8761 ggtcacgccc aagctggggt tgaacgtgac ccgctatgcc atcgacccga acacggcggg
     8821 cttcgagggc cagacccgga cgctacccat tttcaccacc gacagcagcg tagtgctcga
     8881 gcgcccggcg gtgatcggcg gcacatcctt cctgcaaaca ctcgagcccc ggctctacta
     8941 tgtctacatc ccatacaaag accagagccg cataccggtg ttcgacagcg ggcagcagga
     9001 catcaatttg tcgaccattt attccgagaa ccagtacgcg ggatgggatc gtatcaacga
     9061 cgccaaccag gtgacgctcg gcgtcacctc gcgcctgata tcagccgaca gcggtgcaga
     9121 actcctgagc gggggggtgg cacagcgcta ttacttcgag acccagcgtg tcacgctgcc
     9181 gggcgtcccc gcgcgcacga gcacgagttc cgacctgctt gccgtaattt ccggagcggt
     9241 ggcgcctcac tggaccgcgg acgcgggctg gcagtacagc accaattttt cccagactca
     9301 gaaattcaat atcggcacgc gttatcagcc gcagcccgga aaggtgctca acctgagcta
     9361 ccgcgagacg ctcaatgtgc tgcgccagac cgatatttcc gctcaatggc cggtcggc
//
LOCUS       DBNGSG010000337         3654 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2147906, whole genome shotgun sequence.
ACCESSION   DBNGSG010000337 DBNGSG010000000
VERSION     DBNGSG010000337.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2147906"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="AC158Q_08790"
     CDS             <1..413
                     /locus_tag="AC158Q_08790"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3339912.1"
                     /translation="SNGKAAVSLVAHDENEIVGHILFSPVTLEPPAAVMAFGLAPMAV
                     LPGHEKHGVGRRLVQNGLAECHSRGACMVVVLGDPPYYGRFGFERASRHGLRNEFGVE
                     EEFMVFMLDARAHPEPGTLVKYAPEFSALPKTQG"
     gene            complement(504..2210)
                     /locus_tag="AC158Q_08795"
     CDS             complement(504..2210)
                     /locus_tag="AC158Q_08795"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3339913.1"
                     /translation="MATAQAGVLHVKGQTTPVISLIGFGLIWLFLILFVLYPLTRIFY
                     DAFTNEAGAFTIANFFEFFTDRFYLRSLWNSLLLGVAAVISTSVVGIAVAFLIIRFEF
                     PGRNLFSYLTMLPMILPPLVGVLGFVFILGRAGTVNVLLYDWFGLESPVNFMYGMHGV
                     LLVETVHLFPLMTLSILDSMSKVDPSLEEAAQGMGASGWRRFRDVTLPLTTPGYISGA
                     LLVFIWTFADFVTPLVVGVQDLLAVQAYLNIVQFVDRRIFRMGIVISALLVVLAIVFV
                     LLARHYVAIKDYSSLAYSKVERRQLGPVKRWLAVGFLVALLIVSAIPQVGVVLAAVGK
                     GWALTPLPIHYTLDYFRQVSIETPKFIINSIMFSGLAVLICLVIGVPMAWIMGRTKAP
                     GRGAMDALTTLILAIPGTAIGIAYIRAFHYPLPGIDLALTSMWFVLPLVLAVRRLPYT
                     VRGSYSSLLLVHQSMEEAAANVGATKLRTFRDITVPLVWKGILVGALFSFIMSIQEAS
                     STLFLTLGGWEMIPVGIFTYYIAGSHSQAAALGVILIVMCATSLFVVNRVAGTRVGGL
                     FG"
     gene            complement(2216..3292)
                     /locus_tag="AC158Q_08800"
     CDS             complement(2216..3292)
                     /locus_tag="AC158Q_08800"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3339914.1"
                     /translation="MRISVEHLTKRFGPLAVVSDVSLSIQEGELFTLLGPSGCGKTTL
                     LRLIAGFYGPDAGEVRFGDRVVNAVPPHERGIGMVFQNYALWPHMTVSGNISYGLKLR
                     KVPAAEMATRVQGVLEKVKLGGLGDRYPGQLSGGQQQRVALARALVLNPQILLLDEPL
                     SNLDAKIRVQVRAEIRKLQRELGITTIYVTHDQDEALTLSDRIAVFNQGKVFQVGPPK
                     ALYERPANRFVADFIGINNFIEGKVVSVDGAQRRLSAESAFGELAAICDAPLKAGDRC
                     LICVRPENIAMEGQPGGGVNRVSGKISFAAYLGNTLRYDVDLGQGIIFKTDVRDPWHH
                     ELLAMGTAVTLSCPVTSTLAIPAE"
     gene            complement(3317..>3654)
                     /locus_tag="AC158Q_08805"
     CDS             complement(3317..>3654)
                     /locus_tag="AC158Q_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339915.1"
                     /translation="TAWVTPEPMAIIAGTRRPNAARAFVEYVLSERGQRVAMERGVFP
                     ITPKYRVQGKPGSQAEMAVEFTGGLRSYFDVDVTNIYDDDIAQKRYQDVNAQYRKDIE
                     AVAEELKRK"
BASE COUNT          705 a         1201 c         1176 g          572 t
ORIGIN      
        1 gctccaacgg caaggccgcg gtatcgctcg tcgcgcacga tgaaaacgag atcgtcgggc
       61 acatcctgtt cagccccgtg acgctcgaac cgcccgccgc cgtcatggcg ttcgggctcg
      121 cgccgatggc ggtcctgccc ggccacgaga agcatggcgt gggacggcgc ctggtccaga
      181 acggactcgc cgagtgccac tcgcgcggcg cctgcatggt ggtcgtgctg ggagacccgc
      241 cgtactacgg ccgcttcggc ttcgagcgcg cgagccgtca cggcctgcgc aacgagttcg
      301 gcgtggaaga ggaattcatg gtgttcatgc tcgacgcgcg cgcgcatccc gagccgggaa
      361 cgctggtcaa gtacgcgccc gagttctcag cgctgccgaa aacccaggga tgaaccgcaa
      421 ggcaggaatc acaaggcaaa aggcaaaagg aaaaacccgc caggcattat cttgttgtgc
      481 cttgtgcgtt ctgccttccg gcgtcaaccg aacagcccgc ccacccgcgt gcccgcgacg
      541 cggttgacga cgaacaggct cgtggcgcac atcacgatca ggatcacgcc cagcgcagcc
      601 gcctggctgt gggaccccgc gatgtagtaa gtgaagatgc cgacgggaat catctcccag
      661 ccgcccagcg tcaggaacag ggtggaggac gcctcctgga tcgacatgat gaaggagaac
      721 agcgcgccca cgaggatgcc cttccagacc agcggcacgg tgatgtcacg gaacgtccgc
      781 agcttcgtgg cgccgacatt cgcggccgcc tcttccatcg actggtgcac cagcagcagc
      841 gaggaatagc tgcctctcac cgtgtacggc aaccggcgca cggcgagcac cagcggaagg
      901 acgaaccaca tgctggtcaa cgcgaggtct atgcccggca gcgggtagtg aaaggcgcgt
      961 atgtaggcga tgccgatggc agtgcccgga atggcgagga tcagcgtcgt gagcgcgtcc
     1021 atcgcgcccc gtcccggcgc cttggtccgc cccatgatcc acgccatcgg cacgccgatg
     1081 acgaggcaga tcagcacggc cagcccggag aacatgatcg agttgatgat gaacttgggc
     1141 gtctcgatgc tcacctgccg gaagtagtcc agggtgtagt gaatcgggag cggagtcagc
     1201 gcccagccct tgccgaccgc ggccagcacc acgccgacct gcgggatggc ggacacgatc
     1261 aggagggcca cgagaaagcc cacggccagc cagcgcttga ccggtcccag ctgccgccgc
     1321 tccactttcg agtaggcgag cgagctgtag tccttgatcg cgacgtagtg ccgcgccagg
     1381 agcacgaaca cgatggcgag caccacgagc agcgccgaga tcacgatgcc catgcggaaa
     1441 atgcgccggt cgacgaactg gacgatgttg aggtaggcct gcacggcgag caggtcctgc
     1501 acgccgacca ccagcggcgt gacgaagtcc gcgaaggtcc agatgaaaac cagcagcgcg
     1561 ccggagatgt agccgggcgt cgtcaacggc agcgtcacgt cccggaagcg ccgccacccc
     1621 gacgctccca tgccctgggc ggcctcctcg agcgacgggt ccaccttgga catggaatcg
     1681 aggatggaca gcgtcatcag cgggaacagg tgcaccgtct cgaccagcag cacgccgtgc
     1741 attccgtaca tgaaattgac gggactctcg aggccgaacc agtcgtacag cagcacattc
     1801 accgtccctg cccggcccag tatgaagacg aagcccagca cgccgaccag cggcggcagg
     1861 atcatcggca gcatcgtcag gtacgagaac agattacggc ccgggaattc gaagcggatg
     1921 atgagaaacg ccacggcgat cccgaccacc gaggtcgaga tgaccgcggc cacgcccagc
     1981 aacagcgaat tccacagcga tcgcaggtag aaccggtccg tgaagaactc gaaaaagttg
     2041 gctatcgtga aagcccccgc ctcgttggta aacgcgtcgt agaagatccg cgtgagcggg
     2101 tacaacacga agagaatgag aaacagccag atcaggccga acccgatgag ggatatcacc
     2161 ggcgtggtct gccccttcac gtggaggacg cctgcctgcg ccgtggccat gaatgctatt
     2221 ccgcggggat ggcgagcgtg ctggtgaccg ggcagctcag ggtgaccgcc gtgcccattg
     2281 ccagcagctc gtgatgccag ggatcgcgga catcggtctt gaagatgatg ccctgcccca
     2341 ggtccacgtc gtagcgcagc gtgttgccga gataggcggc gaaggaaatc ttcccgctca
     2401 cgcgattgac cccgccaccg ggctgcccct ccatcgcgat gttttccggc cgcacgcaga
     2461 tcaagcagcg gtcgcccgcc ttgagcggcg cgtcgcaaat ggcggccagc tcgccgaacg
     2521 ccgattccgc gctcaacctg cgctgagcgc cgtcgaccga gaccaccttg ccttcgatga
     2581 aattgttgat gccgatgaaa tccgccacga agcggttggc gggccgctcg taaagcgcct
     2641 tgggcggccc cacctggaag accttcccct ggttgaatac cgcgatgcgg tcggacaggg
     2701 tcagggcctc gtcctggtcg tgggtgacgt agatcgtggt gatgcccagt tccctctgca
     2761 gcttgcggat ctcggcccgc acctgcacgc ggatcttggc gtcgaggttg gaaagcggct
     2821 cgtcgagcag caggatctgg ggattcagca ccagcgcgcg cgccagcgcc acgcgctgct
     2881 gctgcccgcc cgacagctgg cccgggtagc gatcccccag cccgcccagc ttcaccttct
     2941 ccagcacccc ttggacccgg gttgccatct cggccgccgg aaccttgcgc agcttgagcc
     3001 cgtaggagat gttcccggac accgtcatgt gcggccacag ggcgtagttc tggaacacca
     3061 tgccgatgcc gcgctcatgc ggcggcacgg cgttgacgac gcggtcgccg aaacggactt
     3121 cccccgcgtc ggggccgtaa aagccggcta tgagtcgtaa cagcgtggtc ttgccgcagc
     3181 cggaaggacc caggagggtg aacagttcac cctcctggat ggagagactg acatcgctga
     3241 caaccgccag cggcccgaat cgcttggtga gatgctcgac cgatatgcgc atgtccgggc
     3301 cgcgggcgat tccttgttac ttgcgcttca actcttccgc gaccgcctcg atgtccttgc
     3361 ggtattgcgc gttgacgtcc tggtagcgct tctgggcgat gtcgtcgtcg tagatattgg
     3421 tcacgtccac gtcgaaatag gagcgcagcc cgccggtgaa ctcgaccgcc atttccgcct
     3481 gggagccggg cttgccctgg acgcggtact tgggcgtgat cgggaacacg ccgcgctcca
     3541 tggccaccct ctggccgcgc tcggacagca cgtattcgac gaacgcgcgc gccgcgttcg
     3601 gccgccgcgt gcccgctatg atggccatcg gctcaggcgt gacccatgcg gtct
//
LOCUS       DBNGSG010000338        10202 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_109990, whole genome shotgun sequence.
ACCESSION   DBNGSG010000338 DBNGSG010000000
VERSION     DBNGSG010000338.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 10202)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10202)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10202
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_109990"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="AC158Q_08810"
     CDS             <1..188
                     /locus_tag="AC158Q_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HIR3339916.1"
                     /translation="CPTFCGTGIAARHVDLRPYVLCGKTISVVPGALTRVALREGSLV
                     VNSSQGGGTKDTWVLEE"
     gene            307..1302
                     /locus_tag="AC158Q_08815"
     CDS             307..1302
                     /locus_tag="AC158Q_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HIR3339917.1"
                     /translation="MLSRVANNLYWMSRHIERAENTARVLDVTYRMSLLPYRTPDQSW
                     ADPWAVPLVINGLATTYYERFAGALTSERVLRFMALDHANPSSIYSCIQSARENARTV
                     RGAITSEMYEDINGLWLEMKNRSSESLFTQGISEFFDWVKMRSHQFRGVTFGTLLRDE
                     AYQFIRLGTFMERADNTARILDVKYHTLLPSVADVGGAVDYYQWGALLRSVSAFDSYR
                     KVYRDAITPMKVAELLILRDDVPRSLHACTKEIYEILRGLCDASSREPERLAGELNAQ
                     LRYGRTEQIIALGLHEYLLSVLDKLSVLNEEIDRHFLVPVHPRQQTQTAAFAASR"
     gene            1421..2146
                     /locus_tag="AC158Q_08820"
     CDS             1421..2146
                     /locus_tag="AC158Q_08820"
                     /inference="COORDINATES: protein motif:HMM:NF012452.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome-type protease"
                     /protein_id="HIR3339918.1"
                     /translation="MLDAGMVFASDTRTNAGVDHVSSYRKMHTFAAEGERVIVMLMSG
                     NLSVTQSTISLLDQNARNPDSNKTVMNVTSMYEVAELIGGALREVRQRDGAYLQQSNV
                     DGSASFIVGGQIRGEPQRLFNVYTEGNFIEATRETPYFQIGESKYGKPVIDRVIQPGT
                     GLLEATKCVLVSFDSTMRSNISVGLPIDLLIYNRNSLRVGFQRHIEESDDYFEMIHKQ
                     WGEGLRRVFAQLPNPDWNISGSA"
     gene            2175..5320
                     /locus_tag="AC158Q_08825"
                     /pseudo
     CDS             2175..5320
                     /locus_tag="AC158Q_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901866.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
     gene            5317..5910
                     /locus_tag="AC158Q_08830"
     CDS             5317..5910
                     /locus_tag="AC158Q_08830"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014722225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HIR3339919.1"
                     /translation="MRRRCPWCEINSQEIDYHDREWGIPVHDDRRWFEFLILEGAQAG
                     LSWDTILKKRASYRRAFARFDPKKVARFDRRKRAALMKNPGIVRNRLKIESAITNARA
                     FLAVQRDFGSFDRYVWQFTGGKPKINRWRAQKQVPASTPESDAMSKDLKQRGFRFVGT
                     TICYALMQATGMVNDHLVACFRYRESTRRKSKGERRK"
     gene            complement(6056..8449)
                     /locus_tag="AC158Q_08835"
     CDS             complement(6056..8449)
                     /locus_tag="AC158Q_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HIR3339920.1"
                     /translation="MRLNVLCFVAGTWWLQQQAVLPATGWVLALGVAGLAATAAPPGT
                     GALRWLRELLVKAACLALGFTWAAWCAQQRLADALPAEWEGRDIAVIGVVAGLPQFYD
                     RGVRFEFDVERVLTPQASAPRRIVLSWWGNPLRDGRPASFPALEPGERWQLTVRLKRP
                     RGTANPHGFDYEAWLFERNIRATGYVRPKAPRERLADSVHGPGYWVERTRSEIRKRIR
                     AALPDAPHAGIITALAIGDQRAIPPEQWQTFTRTGVNHLMSISGLHVTMVSGLVFALV
                     YGLWRRGPRRTLALPAQKAAAAGALGAALLYALLAGFAVPAQRTVYMLAVVAAALWLG
                     VIESASVVLCVALLVVVMIDPWAVLAPGFWLSFGAVAVILYVTTGRIGREHWLASWAR
                     VQIAVTLALIPPLLAMFQQISIVSPLANAVAIPVVSLIVAPLALIGIALPFDLVLDCA
                     HLIMSGCMALLEWLSGLPDAVWQQHAPPAWTVVIAVAALAWLLAPRGLPARWLGMVGL
                     LPLFVAAPNALRAGDVEVVLLDVGQGVSALVRTANRALLYDAGPPFGPAADSGSRIIV
                     PYLRTAGIKRLDGMIVSHDDDDHWGGAASVLQAVPVARLLTSLPDLDPLVVQAQPALR
                     CEAGQLWEWDGVRFEVLHPTSGSYDDPMVKDNDRSCVLRIDAQGGSLLLPADIERRSE
                     EELLVRGRERLRAEILLAPHQGSRTSSTPDFVRAVSPQIVVFPVGYRNRFGHPHRDVL
                     ERYREAGARIYRTDRDGAVTIAISANGAIGVTPYRAVYRRYWQTPLVGDPVPEPEEF"
     gene            8651..9214
                     /locus_tag="AC158Q_08840"
     CDS             8651..9214
                     /locus_tag="AC158Q_08840"
                     /inference="COORDINATES: protein motif:HMM:NF021363.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HIR3339921.1"
                     /translation="MPRKYFRKWLPSHDSIRANRYIAWLGPRLQHHNLWHLHRRSVAG
                     GVAAGLFAGLIPGSNPVQFTAGALLAIAGRVNLPIAVFVTLYSNPLTIVPLYYVAFRL
                     GQLVLLQSGGALPSLAFELEGAGFREWIPAALAWLSSVGKPLVIGLPLLALLLALAGY
                     FMVDWTWRIYVHCTWARRHRRRARMKT"
     gene            9211..9831
                     /locus_tag="AC158Q_08845"
     CDS             9211..9831
                     /locus_tag="AC158Q_08845"
                     /EC_number="3.2.2.-"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase family protein"
                     /protein_id="HIR3339922.1"
                     /translation="MTTPDYWEQAKCELAERDAVLRGLVERFQGLALGSRGDAFSTLA
                     RAIVGQQISVKAAQSVWNRLSDRVVTMTPESLGRCRIPALRACGLSGQKAGYLKDLAG
                     KFRDGTLEPARWPALGDEVLIGELTRVKGIGRWTAEMYLMFHLARPDVLPVADLGLQR
                     AMRLHYNRGRTLSLARMQKIGATWAPWRSVATWYMWRSLDPIPVEY"
     gene            9931..>10202
                     /locus_tag="AC158Q_08850"
     CDS             9931..>10202
                     /locus_tag="AC158Q_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339923.1"
                     /translation="MNHGEFTSAYAHGKVKVEIDPKAAARFLSARLLLPLVMMPVLGL
                     GVALALSGWIYTGLAILAAGIVAPQLIKRGAPHFILTQALQDAAFY"
BASE COUNT         1740 a         3605 c         3268 g         1589 t
ORIGIN      
        1 cctgccccac gttctgcggc accggcatcg ccgcgcggca tgtcgacctg cgcccgtatg
       61 ttctctgcgg caagacgatt tcggtcgtgc ccggcgcgct cacccgcgtc gcgctgcggg
      121 aaggctcgct cgtcgtcaat tcctcgcagg gcggcggcac caaggacacg tgggtgctgg
      181 aagaatgaac atgaaggcgg aagtcgaaag tcgaaaggcg aaagtaacaa cagccggccg
      241 ccgcgagcgg cgtattgtcg ttcttcgttc ttccttccga ctttcgactt tcgactttcg
      301 acttgtatgt tgagccgcgt cgccaacaac ctctactgga tgtcgcgcca tatcgagcgc
      361 gcggagaata ccgcgcgcgt gctggatgtg acctaccgca tgtcgctgct gccgtaccgc
      421 acccccgacc agtcgtgggc cgacccctgg gcggtgccgc tcgtcatcaa cggactcgcc
      481 acgacctact acgagcgctt cgccggcgcg ctcacgtccg agcgcgtgct gcgcttcatg
      541 gcgctcgatc acgccaatcc ttccagcatc tactcctgca tccagtcggc gcgcgagaac
      601 gcccgcaccg tgcgcggcgc gatcacctcg gaaatgtacg aggacatcaa cggcctctgg
      661 ctcgagatga agaaccgctc cagcgaaagc ctgttcacgc agggcatcag cgagttcttc
      721 gactgggtga aaatgcgctc gcaccagttc cgcggcgtca ctttcggcac cctgctgcgc
      781 gacgaggcct accagttcat ccggctcggg accttcatgg agcgcgctga caatacggca
      841 cgcatcctcg acgtcaagta ccacacgctg ctgccgagcg tcgcggacgt gggcggtgcc
      901 gtggactatt accagtgggg cgcgctgctg cgctcggtga gcgcgttcga ctcctaccgc
      961 aaggtctacc gcgacgcgat cacgccgatg aaggtggcgg aactgctgat cctgcgcgac
     1021 gacgtgccgc gatcgctgca cgcgtgcacg aaggaaatct acgagatcct gcgcggactg
     1081 tgcgacgcgt cgtcgcgcga gccggagcgc ctcgcgggcg aactgaacgc gcaactgcgc
     1141 tacgggcgca ccgagcagat catcgcgctc ggcctgcacg agtacctgct gagcgtgctc
     1201 gacaagctct cggtgctgaa cgaggaaatc gaccgtcatt tcctggtgcc ggttcacccc
     1261 cggcagcaaa cccagacggc cgcgttcgcc gccagccgct agcgccgctc ccttcccccg
     1321 tcatcatccc tcgtccggcg gcttgtcagc ccgcggcggt tacaataagc tttcccgcct
     1381 ttgattacgt aatcacccga tgacctattg tgtaggcacc atgctggacg ccgggatggt
     1441 gttcgcgtcc gacacgcgca ccaatgccgg cgtggaccac gtctcgagct accgcaagat
     1501 gcataccttc gcagccgagg gcgagcgcgt catcgtcatg ctgatgtcgg gcaacctctc
     1561 ggtgacgcag agcacgatca gcctgctcga ccaaaacgcc cgcaacccgg actcgaacaa
     1621 gaccgtgatg aacgtcacct ccatgtacga agtggcggag ctgatcggcg gcgcgctgcg
     1681 cgaagtcagg cagcgcgacg gcgcctacct tcagcaaagc aacgtggacg gcagcgcctc
     1741 cttcatcgtc ggcgggcaga tccgcggcga gccgcagcgg ctgttcaacg tctacaccga
     1801 gggcaatttc atcgaggcca cccgcgaaac gccgtatttc cagatcggcg agagcaagta
     1861 cggcaagccg gtgatcgacc gcgtcatcca gcccggcacc gggctgctgg aagcgaccaa
     1921 gtgcgtgctg gtgtcgttcg actccaccat gcgctccaac atctcggtcg gcctgccgat
     1981 cgatctgctg atttacaacc gcaacagcct gcgcgtcggt ttccagcgcc atatcgagga
     2041 gagcgacgac tacttcgaga tgatccacaa gcagtggggc gaaggcctgc gccgggtgtt
     2101 cgcgcagctg ccgaatcccg attggaatat ctcgggctcc gcttgaggcc gggcgcggtg
     2161 ccgcgccggc cggcatgagc atgctcgttt cgctgagaca ctccactcgc tacctctacg
     2221 accgcccggt cacgcttggc ccgcacgaga tccggctgaa gccggtgccg ggttgccgca
     2281 ccccggtgtc ttcctacact ctggcggtgc gcccggaccg gcataccctg cacagtcact
     2341 acgacgcagc gggaaatccc gtcgcgcggg tgctgttcca ggacaggatc acccggctgg
     2401 aagtggacgt ggcgctcacc gccgatctcg cgccgttcaa cccgttcgat tttctggtcg
     2461 agcccggcgc agccagctac ccgctcgcct accccggcgc ggcccgcgcc gaactcgcgc
     2521 cctacctctg cgccgacgag agcggcgggc ggatgcggcg ctggctgcag gatttccgat
     2581 cttcggacaa ccccgatggc cgcgacaccg tcgatctcct ggtgcgcatc aacgaacgga
     2641 tcaggcggga catcggctat gtcacgcgca tggaacaggg cgtccagtcc tgcgagcaaa
     2701 cgctcgaact gcgcagcggc tcatgccgcg atagcggctg gctgctcgtc caggttctgc
     2761 gccatttcgg attcgctgcg cgattcgtgt cgggctacct gatccagctg tcgggcagcg
     2821 ccgccgacgc ccccaggacc gacggcgcgg atctccacgc ctgggccgag gcttacctgc
     2881 cgggggccgg ctggatcggc ttcgacccga cttccggcat gctcgctgcg gagggccaca
     2941 tcccgcttgc gcgcgcggcc acgcccgcgc tcgccgcgcc ggtaaccggc tcggtcgagc
     3001 cgtgcaattc gcagatgcaa tcctcgatgt cggtgcagcg cacgtgagcc cgtactccga
     3061 agcgcaatgg cgtgccatcg acgggctcgc ccgccgcgtg gacgccgacc tgaagtccct
     3121 cggcgtgaag ctcacgcagg gcggcgagcc gaccttcgtc gcggctgccg ggggcgaggc
     3181 gccggagtgg aaccattcgg cgatgggacc ggacaagcgg cggttcgcgg ggcggctgct
     3241 gcgccggctg cacgcccgct tcgcgccggc gggactgatg cacttcgggc agggcaagtg
     3301 gtacccggga gagccgttgc cgcgctgggc gctcggcctg atctggcgca aggacgcgac
     3361 cgccctgtgg cgggacccgc aactcatcgc gaacgaggaa gccggcacga gtcacggcat
     3421 ggaggacgcg cgacggctga tcggtgccct cgcggagcgt ctcggcatcg cggcgaatct
     3481 catcgtgccg gcttacgagg atccctggcc ggcactcgac tccgaaagcg gcctgccgga
     3541 gggcgtcgat ccgctgcgca gcgatcttgg aaacgccgac gagcgcgcgc ggctggcgcg
     3601 actgctgcgg caaggactgg ccagtgtcgc cggctatgtc ctcccgctgc gcacagagcc
     3661 acgagccgat gcccgcggcc ccaccgcgtg gcgcagcagc cgctggccgt tgcggcgcga
     3721 gcgcctttat ctcatgcccg gagaatcgcc catcggctac cggctcccgc tcgatacgct
     3781 gccggagacc gaacccgccc gcatcgcatt gtgcgtggaa gcgcggggag gcctgctgca
     3841 tgtgttcgtg ccgccgcttg acacgctcga cgattacctt gcgctgatcg cggccgtcga
     3901 gatgagcgcc cgcgaactgg gactgcccgt gctgccggaa ggctacgagc ctccccccga
     3961 cgacacgctg atcacgctga ggatcacgcc cgatcccggc gtgatcgaag tcaatgtgca
     4021 cccggccgcc gactggaacg aactgtccac gatcaccgcc gccgtgtacg aggaagcgcg
     4081 cggcctcggg ctcgccgcgg aaaagttcat gcgcgacggg cgcaggaccg gcaccggcgg
     4141 cggcagccat atcacgctcg gcggcgccgc accggcggaa agcccgctgc tgcgacgtcc
     4201 cgacctgctg gcgagcctca tcgcctactg gcagaaccac ccggcgctgt cctacctctt
     4261 ctccggccag tacatcggcc ccacctgcca ggcgccgcgc gtggacgagg cgcgcgagga
     4321 cagcctctac gagctggaaa tcgcctttca gcagctcgcg cgtctgcagc agtcgggcgc
     4381 gcccgccccc gagcgcatcg accgcctgct gcgcaacctg ctggtggaca ttaccggcaa
     4441 cacgcaccgc gcggagttct gcatcgacaa gctctactcc cccgacggcc cggccgggcg
     4501 cctcgggctg ctcgagctgc gcgcgttcga aatggcgccg cacagccgca tggccctggt
     4561 gcagttcctg ctggtccgcg cgctggtcgc gcaattctgg cagacgccct accgcggccg
     4621 gctcatccgg tggggcaccg cgctgcacga ccgctacatg ctgccgcact atctcgccgc
     4681 cgacctgcgc gaagtcgtgt cgggcctcaa cgcggcgggc cgggctttcg atttcgagtg
     4741 gctcgagccg tttttcgagt tccgcttccc gctctgcggc accgtcgcct gcgggcccgt
     4801 cacgctcgag ctgcggcacg cgctcgagcc ctggccggtg ctcggcgaag cggccgccgt
     4861 cagcggcacc tcccgcgccg tcgacgcctc cctggacaga gtgcaaatca gggtgtccgg
     4921 catggatggc gcgcatcatg ccgttttgtg caacggccgc cgtgtgccgc tgcaacccac
     4981 cggcaccgcg ggcgaatacg tcgccggcgt gcgttaccgg gcgcggctct tcccgtccgt
     5041 gcttcacccc accatcggcg tacacgcgcc gctcgccgtc gatgtcgtgg acacgcgcat
     5101 tcagcgcgcg ctgggcggat gcacttatca cgcgatgggg cccgggggga aggaatacga
     5161 gcggccgccc gccggcgaaa gcgaagcggc ggcgcggcgc aagacgcgct tcatcacgcg
     5221 cggcccggac ggccgaaccc tggccatccc gcccgaatcg cccaatccct tcgcgccctg
     5281 caccctggat ctgcgcctga gcccggagtc cccgtcatga ggcgccgttg tccctggtgt
     5341 gaaatcaatt cgcaggaaat tgactatcac gaccgggaat ggggcatccc cgtccacgac
     5401 gatcggcgct ggttcgagtt cctgatcctg gaaggcgcgc aggcggggct ctcgtgggac
     5461 accatcctga aaaagcgcgc cagctaccgc cgcgcctttg cgcgcttcga cccgaaaaag
     5521 gtcgcccgct tcgaccgcag gaaacgcgct gccctgatga aaaatcccgg catcgttcgc
     5581 aaccgtctca agatcgagtc cgccatcacc aacgccaggg ccttcctcgc cgtgcagcgc
     5641 gatttcggca gcttcgatcg ctacgtctgg caattcaccg gcggcaagcc gaagatcaac
     5701 cgctggcgcg cccaaaaaca ggtgcccgcc tccacgcccg agtccgacgc catgagcaag
     5761 gaccttaagc agcgcggctt ccgcttcgtc ggcacgacca tctgctacgc cttgatgcag
     5821 gcgacgggga tggtcaacga ccatctcgtc gcgtgctttc gctaccgcga aagcacaagg
     5881 cgaaagtcga aaggcgaaag gcggaagtaa aatccgtcca ccagagttct ttagaactgg
     5941 ccaaaccgag ccaaaacgtc atccccgcgc aagcggggat ccaggcaagt tttagattcc
     6001 cgcctccgcg ggaatgacag tattttcttc cgcctttcga ctttcgcctt ccgccttaga
     6061 actcttccgg ctccggcacc gggtccccga cgagcggcgt ctgccagtaa cgacgataaa
     6121 ccgcgcgata gggcgtcacg ccgattgcgc cattcgcgct gatcgcaatc gtcacggcgc
     6181 catcgcgatc ggtgcggtag atccgcgccc cggcttcccg gtaacgctcc aggacgtcgc
     6241 ggtggggatg gccgaaccgg ttgcggtaac ccaccggaaa taccacgatc tgcggactga
     6301 ccgcccgcac gaagtccggg gtcgaggagg tccggctgcc ctggtgcggc gccagcaata
     6361 tctccgcgcg caatcgctcg cggccgcgca ccagcaactc ctcctccgag cggcgctcga
     6421 tgtcggcggg aagcaggagg cttccgccct gcgcgtcgat cctgagcacg caactgcggt
     6481 cgttgtcctt gaccatggga tcgtcgtaac tgccgctcgt cgggtgcagc acctcgaagc
     6541 gcacgccatc ccattcccat agctggccgg cttcgcagcg cagggccggt tgcgcctgca
     6601 ccaccagggg atcgaggtcg ggcagcgaag tcagcagccg ggccaccggc acggcctgca
     6661 acaccgacgc cgcaccgccc cagtgatcgt catcgtcgtg gctgacgatc atgccgtcca
     6721 gccgcttgat ccccgcggtg cgcaggtacg gcacgatgat gcggcttccg ctatcggcgg
     6781 cgggcccgaa cggagggccg gcatcgtaga gcagcgcgcg gttcgcggtg cgcaccagcg
     6841 cggatacgcc ctgacccacg tccagcaaaa ccacctcgac atcgcctgcc ctcaacgcat
     6901 tgggggccgc cacgaacagc ggcagcaacc cgaccatccc cagccagcgc gcgggcagcc
     6961 cgcgcggcgc aagcagccag gccagcgccg ccaccgcgat gaccacggtc cacgccgggg
     7021 gcgcgtgctg ttgccacacg gcgtcgggaa gcccgctcaa ccattcgagc agcgccatgc
     7081 aaccgctcat gatcagatgg gcgcagtcga gaacgagatc gaacggcaac gcgatcccga
     7141 tcagcgccag cggcgcgacg atcaggctca ccaccggaat cgccaccgcg ttggcgagcg
     7201 gcgagacgat cgagatctgc tggaacatcg ccagcagcgg cggaatcaag gccagcgtca
     7261 ccgcgatctg caccctggcc cagctcgcaa gccaatgctc gcggccgatg cggccggtcg
     7321 tgacgtacag gatcacggcc accgcgccaa aggaaagcca gaatcccggc gccagcaccg
     7381 cccaaggatc gatcatcacg acgacgagga gcgccacgca cagcacgacc gacgccgatt
     7441 cgatgacgcc cagccacaac gcggcggcga ccacggccag catgtaaacc gtgcgctggg
     7501 ccggcacggc gaaacccgcc agcagcgcgt agagcagcgc ggcccccagc gctccggcgg
     7561 cagcagcttt ctgggcaggc aaggcaagcg tccggcgcgg accgcgccgc cacaggccat
     7621 agaccagggc gaatacgagg cccgagacca tggtgacgtg caggcccgag atgctcatca
     7681 ggtggttgac gccggtgcgg gtgaacgttt gccattgttc cggcggaatc gcgcgctggt
     7741 ccccgatcgc cagcgcggta atgatgccgg cgtgcggcgc atccggcagc gcggcccgta
     7801 tgcgcttgcg gatctccgaa cgggtgcgct ccacccaata accgggccca tgcacactat
     7861 ccgccagccg ctcgcggggc gccttcggcc gcacataacc ggtcgcccgg atgttgcgct
     7921 cgaacagcca cgcctcgtaa tcgaaaccgt ggggattggc ggtgccgcgg gggcgcttca
     7981 accgcaccgt cagctgccag cgctcgcccg gctcgagcgc cggaaacgag gcggggcggc
     8041 catcgcgcag tggattgccc caccacgaga gcacgatgcg ccgtggcgcg ctcgcctgag
     8101 gcgtcaacac gcgctcgacg tcaaactcga atcgtacgcc ccggtcgtag aactggggca
     8161 gtccggccac cacgccgatg accgcgatat cgcggccctc ccattccgcc ggcagggcat
     8221 cggcgagcct ttgctgcgcg caccatgccg cccaggtaaa tcccagggcg aggcaagccg
     8281 ctttcacaag cagctcgcgt agccagcgca gtgccccggt cccgggcggc gcagcagtcg
     8341 cggcaagccc ggccacgccc agcgccaaca cccacccggt agcgggcagc acggcctgct
     8401 gctgcagcca ccaggtgccg gcaacgaagc agagaacgtt cagcctcatg gccccgtaac
     8461 gcgcgacagc acgggccgga ggaccggaag gcaaggcgaa aggcggaagg cggaagcaaa
     8521 gcctctcggt caaaacgtca tctccgcgca agcggagatc caggagaacg ctggataccc
     8581 gcctccgcag gaatgacgga tatacctttc gcccttcgcc tttcgacttt cgccttcagt
     8641 aaaatgccgc atgccgcgca agtacttccg caagtggctt ccgagccacg attccatccg
     8701 ggcaaaccgg tacatcgcct ggctcgggcc acggctgcag caccacaacc tctggcacct
     8761 gcatcgccgc tccgtggcgg gcggcgtggc ggccggcctg ttcgcgggtc tcatccccgg
     8821 cagcaacccg gtgcagttca ccgcgggcgc gctgctggcg atcgccggcc gggtcaacct
     8881 gccgatcgcg gtgttcgtga cgctctactc caaccccctc accatcgtgc cgctctatta
     8941 cgtggcattc cggctcgggc agctcgtgct gcttcaaagc ggcggcgcgc tgccgtcgct
     9001 tgcgttcgag ctggaaggcg caggcttcag ggaatggata cccgccgcgc tggcctggct
     9061 gagttcggtc ggcaagccgc tggtgatcgg gctgccgctg ctcgcgctgc tgctcgcgct
     9121 cgccggttac ttcatggtgg actggacgtg gcggatctac gtgcactgca cctgggcgcg
     9181 ccgccaccgg cgccgcgcca ggatgaaaac atgacaacac cggactactg ggagcaggcg
     9241 aagtgcgagc tcgccgagcg ggacgcggtg ctgcgcggac tggtcgagcg cttccagggc
     9301 ctcgcgctcg gcagccgtgg cgacgccttc tccacgctcg cgcgcgccat cgtcggccag
     9361 cagatctcgg tgaaggcggc gcaaagcgtg tggaaccggc tttccgaccg ggtggtcaca
     9421 atgactccgg aatcgttggg ccgctgccgc atacccgccc tgcgagcctg cggcctgtcc
     9481 gggcagaagg ccggctacct gaaagacctg gccggaaaat tccgggacgg cacgctcgag
     9541 cccgcgcgct ggcccgcgct gggtgacgaa gtcctgatcg gagagctgac tcgggtgaaa
     9601 ggcatcggcc gctggaccgc cgagatgtat ctcatgtttc acctcgcgcg cccggacgtg
     9661 ctgccggttg cggacctggg gctgcagcgc gcaatgcggc tgcattacaa tcgcggccgc
     9721 accctgtcgc tcgcgcgcat gcagaagatc ggcgccacct gggcgccgtg gcgctcggtg
     9781 gccacctggt atatgtggag gtcgctcgat cccataccgg tcgagtactg agaaacgtga
     9841 caggtgataa gtgataggtg ataggagttt tactatctcc gcgtcgcggg agccttatca
     9901 cttatcacct ctcactcatc acgcctcatc atgaatcacg gcgaattcac ctccgcctac
     9961 gcgcacggca aggtcaaggt cgagatcgac ccgaaggcgg cggcgcgctt tctgtcggcg
    10021 cgcctgctgc tgccgttggt gatgatgccg gtgctgggac tgggcgtggc actggcgttg
    10081 agcggctgga tctacacggg tctcgcgatc ctcgccgccg gcatcgtcgc gccccagctc
    10141 atcaagcgcg gcgcgccgca cttcatactg acccaggcgc tgcaggacgc ggcattctac
    10201 ga
//
LOCUS       DBNGSG010000339         1960 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_620295, whole genome shotgun sequence.
ACCESSION   DBNGSG010000339 DBNGSG010000000
VERSION     DBNGSG010000339.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_620295"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..1034)
                     /gene="hemB"
                     /locus_tag="AC158Q_08855"
     CDS             complement(27..1034)
                     /gene="hemB"
                     /locus_tag="AC158Q_08855"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HIR3339924.1"
                     /translation="MQLYGRFPQRRMRRMRRDAFSRRLRREHRLTTDDLIYPIFVIEG
                     RSRSEPVPSMPGIRRHTVDRLLAHAEQCARLGIPAVAVFPLIPRELKSADGREAINPK
                     GLVPRAVAALKKRFPELGVITDVALDPYTTHGHDGVIDRGGHILNDETIAILEKQAVV
                     CAASGVDVVAPSDMMDGRIGAIRGALDRNKFILTRILAYSAKYASVLSGPFRDAVGSA
                     RSLGRGTKHAYQMDYANSDEALWEVALDLEEGADMVMVKPGLPYLDIVRRVKEQFRAP
                     TYAYQVSGEYSMIKAAAARGWLDERDCVMETLTAFKRAGADGILTYFALDAAGWLKAG
                     S"
     gene            1398..>1960
                     /locus_tag="AC158Q_08860"
     CDS             1398..>1960
                     /locus_tag="AC158Q_08860"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3339925.1"
                     /translation="MKSWWLLMLGSGAFVPAVALPQVVPPADTAPAREIVVKVCASCH
                     AEDGNSTLPVNPVLAGQHADYTLKQLQNFRPRNGKPAERVNAVMAGMVANLSGEELRS
                     LAAYFELQKPRLRAARDPDLVRLGQAIYRGGIPAKSVAACTACHGPNGSGIPAQFPRL
                     AGQFAEYTAAQLRAFRAGERANDPNRM"
BASE COUNT          321 a          661 c          642 g          336 t
ORIGIN      
        1 tggaacagct cctggccgaa gcgtgatcag ctgcctgcct tgagccaccc cgccgcatcg
       61 agcgcgaaat aggtgaggat cccgtcggcg ccggcgcgct tgaacgcggt gagcgtctcc
      121 atcacgcagt cgcgctcatc cagccagccg cgcgcggctg cggccttgat catcgagtat
      181 tcgccactca cctggtaggc ataggtgggc gcccggaact gctccttcac ccgccgcacg
      241 atgtcgagat agggcagccc gggcttcacc atgaccatgt ccgcgccttc ctcgaggtcg
      301 agcgcgacct cccacaacgc ttcgtcggag ttggcgtaat ccatctggta ggcgtgcttg
      361 gtgccgcgcc ccagactcct ggcggagccg acggcgtcgc gaaacggccc ggaaagcacc
      421 gaagcgtatt tcgccgagta ggcgaggatc cgggtgagga tgaacttgtt gcggtcgagc
      481 gcgccgcgga tcgcgccgat gcgaccatcc atcatgtccg aaggcgcgac cacgtccacg
      541 ccggacgcgg cgcacaccac ggcctgcttc tcgaggatcg caatcgtttc gtcgttcagt
      601 atgtggcccc cccggtcgat cacgccgtca tggccgtgcg tggtgtaggg gtcgagcgca
      661 acgtcggtga tcacgccgag ctcggggaag cgcttcttca gcgccgccac cgcgcgcggc
      721 accagcccct tgggattgat ggcctcgcgc ccgtcggccg acttgagctc ccggggaatc
      781 aggggaaaca ccgcgacggc cgggataccg aggcgcgcgc actgctcggc atgcgcgagc
      841 aggcggtcga cggtgtggcg ccggatgccc ggcatcgacg gcaccggctc gctgcggctc
      901 ctgccctcga tcacgaagat gggatagatg aggtcatccg tcgtcagccg atgctcgcgc
      961 cgcagccggc gcgagaatgc gtcgcgccgc atgcggcgca tgcgccgctg gggaaacctc
     1021 ccgtagagct gcataagcct aacctcttga tggcagtaac atttagggtc cgaggggaac
     1081 tccgggccat tataagcgtc acagcatgca gacgatgttg agtcgtctcc cgcttctcct
     1141 ccctgagcgg gcgccttaat ccttattaag gcttttgccc ccggttttac tcccctttaa
     1201 ccgggggttt tttattgccg ttgttctgtg cgtcagcgcg cgcgcgattc tggcgtgatc
     1261 acccggccag tgccggcgag caaccgggca acatcgccta ggtcattgtt atcgcgcccg
     1321 aaactggcgt gcgcagcggc gcctgctata caatagcgcc gccctcgaga cgacacgcgg
     1381 ataacagcgg acccggcatg aaatcctggt ggctcctgat gcttggctcg ggtgcgttcg
     1441 ttccggccgt ggcgctccct caggtagtgc ccccggcgga caccgcgccg gcacgcgaga
     1501 tcgtcgtgaa ggtatgcgcg tcctgccatg ccgaggacgg gaacagcacg ctccccgtca
     1561 acccggtgct ggccggccag catgccgatt acaccctgaa gcagctccag aacttccggc
     1621 cgcgcaacgg caagcccgcc gagcgcgtga atgcggtgat ggccggcatg gtggcgaacc
     1681 tctcgggcga ggaattgcgc agcctcgcgg cctatttcga gctgcaaaag cccaggctgc
     1741 gcgccgcgcg cgacccggac ctcgtccggc tcggccaggc gatctaccgc ggtggcatcc
     1801 cggcgaagag cgtcgcggcc tgcacggcgt gccacgggcc gaatggcagc ggcatcccgg
     1861 cgcagtttcc gcgcctggcg ggacagtttg ccgaatacac tgctgcccag ctgcgggcgt
     1921 ttcgtgccgg ggagcgcgcg aacgatccga atcgcatgat
//
LOCUS       DBNGSG010000340         6442 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4695884, whole genome shotgun sequence.
ACCESSION   DBNGSG010000340 DBNGSG010000000
VERSION     DBNGSG010000340.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6442)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6442)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6442
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4695884"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..973)
                     /locus_tag="AC158Q_08865"
     CDS             complement(92..973)
                     /locus_tag="AC158Q_08865"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HIR3339926.1"
                     /translation="MAPLKVGKLVVIGTGLIGGSFALALKRARAVRRVTGVGRTRRNL
                     ADALRLKIIDETSRDSARAVRDADLVLLATPVGQMPAVMTAIAPHLPAHAVITDAGST
                     KRDVIECARRFLGGHFPRFVPGHPVAGTENSGAVAAFPELFRGRNVILTPQRETTASA
                     TRLVRRAWETCGARVVILDAGEHDSILAGVSHLPHIIAYALVNTLARRRNPARLFGLS
                     AGGLRDTVRIAGSSPEMWADICVANRDAMLAALEDYENELEGMRAAIEAANSAELRRM
                     FSAARNAREKWLVKRQP"
     gene            complement(983..2083)
                     /gene="hisC"
                     /locus_tag="AC158Q_08870"
     CDS             complement(983..2083)
                     /gene="hisC"
                     /locus_tag="AC158Q_08870"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003278019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 -
                     L-histidinol-phosphate:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HIR3339927.1"
                     /translation="MDICDLAPGYIRAIAPYQPGKPISELAREMGLEESGIIKLASNE
                     NPLGVSPLAQRAIEAVLADLARYPDGNGFELKQALSRRLGVAPECIVLGNGSNDVLEM
                     AARAFLAPGLDALYSQHAFAVYPLAVQSAGASGIEVAAKDYGHDLAAMRRAVTPSSRL
                     VFIANPNNPTGTMARAHDLEDFIAALPREVLVVLDEAYNEYLPDDLRPDSIGWLKRFP
                     NLVVTRTFSKVYGLAGLRVGYAVAAVGVADLMNRVRQPFNVNSVALAAATAALEDTDF
                     VRRSRELNRKGMRQLTEGFERFGLEYIPSYGNFVSFRVKDAAGVFQKLLKAGVIVRPI
                     ASYGMPQHLRVSIGLETENKRFLACLEEALAT"
     gene            complement(2186..3250)
                     /gene="pheA"
                     /locus_tag="AC158Q_08875"
     CDS             complement(2186..3250)
                     /gene="pheA"
                     /locus_tag="AC158Q_08875"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HIR3339928.1"
                     /translation="MTSSLKQIRARIDVLDQRLLKLLSARARLAQRIGKLKESAAYRP
                     EREAQVLRGVLAGNRGPLPDAALARLFAEIMSACRALEDSMRVAYLGPQGTFSQEAAV
                     KHFGGAAVLSSCPSIDEVFRQVETGAAGYGVVPVENSTEGAIGRTLDLLLTTPARVCG
                     EVMLPIRQCLMSRSGKLSDVRKVLSHTQSLAQCQQWLARSLPRAEREAVVSNAEAARL
                     AARDRRAAAVGPRMAAELYGLKLIARNIEDDAKNTTRFLVVAQHDAAPSGKDKTSLIL
                     STRNVPGAIHELLTPLAANQVSMTRLESRPSRAGMWEYVFYVDVEGHQRDRNVALALA
                     ELKQKASYLKNLGSYPAAVA"
     gene            complement(3345..4532)
                     /locus_tag="AC158Q_08880"
     CDS             complement(3345..4532)
                     /locus_tag="AC158Q_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HIR3339929.1"
                     /translation="MAEPYKILTLNSISSLGLKRLPSGRYTCGNSIADPDAILVRSHD
                     MHPMEIGPSVKAIGRAGAGVNNIPVDKMSKRGVPVFNAPGANANAVKELVIAGLLVAS
                     RNLVPALRFVDGLKGDDKTLHKLVEEGKKDFAGVELPGHTLGVIGLGKVGSLVADAAI
                     RLGMNVLGYDPEITVDAAWGLPAQVKKAHSIEEVLKASDFVTIHVPLLDVTRNLLNAA
                     RIKLMKSGAILLNFSREGIVDNEAVLEALAAKRLRYYVCDFPARKLAGHPQVIALPHL
                     GASTREAEDNCAVMVVDQVRDFLENGNIVNAVNFPEVTMAREAPYRVGIANANVPNMV
                     GQISTAMAKARLNIHNMLNKSKGEMAYTLVDVDSKVPAKLIEQLGDIEGVLMVRYLPV
                     ESQ"
     gene            complement(4525..5607)
                     /gene="serC"
                     /locus_tag="AC158Q_08885"
     CDS             complement(4525..5607)
                     /gene="serC"
                     /locus_tag="AC158Q_08885"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HIR3339930.1"
                     /translation="MARVFNFGAGPATLPEPVLKLAAEELLDWHGSGMSVMEMSHRGK
                     AFVGIHAQAEADLRELLAIPKNYKVLFLQGGGAGQFSIVPMNLLRGRTTASYVNTGHW
                     STRAIAEAKRYCKVSIAASSEDASFTYAPRQDKWKVDPAAAYVHYTSNETIGGVEFHW
                     TPDTGDIPLVADASSHILSRPLDVSRFGLIYAGAQKNIGPAGMAIVIVRDDLIGQAMP
                     ATPSVFDYKVQAGADSMHNTPPTYSVYMAGLVFQWLKKQGGVKKMEEINIAKAQLVYD
                     YLDQTEFYRSPVAKEDRSRMNIPFSLRSDTLDEEFLQQARACGLSELKGHRAVGGMRA
                     SIYNAMPVEGVKALVEFMKEFERKHG"
     gene            complement(5693..>6442)
                     /locus_tag="AC158Q_08890"
     CDS             complement(5693..>6442)
                     /locus_tag="AC158Q_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018439107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HIR3339931.1"
                     /translation="SRASRGKPIVNLVPLMEHEKITAVLPVSQFDDEHFVFMATAMGT
                     VKKTALSAFSRPMAKGIIAVTLDDGDYLIGVSLTDGKQDVMLFSDEGKAVRFAEDEVR
                     AMGRGAAGVRGMKLARDRHVVSLLISSDEQLSVLTATENGYGKRTLVSEYTRHGRGTQ
                     GIIAIDCSERTGKLLAAELVAPEDEIMLITTGGVLIRTRVSEVREMGRSTQGVRLINL
                     DEGEKLAGLQKIAESDENGESESPGAAKAGQ"
BASE COUNT         1105 a         2135 c         1980 g         1222 t
ORIGIN      
        1 cgagccaggc agcctgaccg tgccgcgggc acgtcggata ggagcaagtt ctaatgagtc
       61 cacggcggtt tccagtcggt gcgggcggcc gctacggctg tctttttacc agccacttct
      121 cgcgcgcgtt gcgagccgcc gaaaacatac gccggagttc ggcggaattt gctgcttcga
      181 tcgccgcacg cattccctcg agctcgtttt cgtagtcctc cagcgccgcc agcatcgcgt
      241 cgcggttcgc gacgcagata tcggcccaca tttcgggcga gctgcccgcg atgcgtacgg
      301 tatcgcgcaa tcctcctgcc gagagcccga acagccgcgc cggattgcgg cggcgcgcca
      361 gcgtgttgac aagcgcgtaa gcaatgatgt gcggcagatg gcttacgccg gcgagtatcg
      421 aatcgtgctc gccggcatcc agtatcacaa cgcgcgcacc gcaggtctcc caggctcgcc
      481 tcaccagtcg cgtcgccgag gcggtggtct cacgctgcgg cgtcaggatc acgtttctgc
      541 cgcgaaaaag ctccgggaat gccgccaccg ccccgctatt ttcggttccg gcgaccggat
      601 ggcccggcac gaaccgggga aagtgcccgc ccaggaagcg gcgggcgcac tcgatgacgt
      661 cgcgcttggt gctgcctgca tcggtaatca cggcatgagc gggcagatga ggcgcaatgg
      721 cggtcatcac ggccggcatc tgcccgaccg gcgtggcgag cagcaccagg tccgcatcgc
      781 gcaccgcgcg cgcggaatcc cgcgacgttt catcgatgat tttcagcctg agcgcgtcgg
      841 caagattgcg gcgcgtgcgc ccgacgcccg tcacccgcct caccgcccgc gcccgcttca
      901 gtgccagcgc gaacgagccg ccgatcagcc ccgtgccgat aacaaccagt tttccgactt
      961 tcagcggcgc catgttgctc actcaggtgg ccaacgcctc ttccaaacac gcaagaaagc
     1021 gcttgttctc cgtttccagt ccgatgctaa cccgcaggtg ctgcggcatg ccgtagctcg
     1081 cgatcggccg cacgatgact cccgccttca gcagcttctg aaacacgccc gccgcatcct
     1141 tgaccctgaa gctgacgaaa ttgccatagg acgggatgta ctcgagaccg aaccgctcga
     1201 atccctcggt cagctggcgc attcctttcc gattgagttc tcggctccgg cgcacgaagt
     1261 ccgtgtcctc cagcgccgcc gtcgccgcag ccagcgcaac gctgttgacg ttgaacggct
     1321 ggcgcacgcg gttcatgagg tcggcgacgc caacggccgc gacggcgtag cctaccctca
     1381 gcccggccag tccgtagacc ttggaaaacg tgcgcgtcac caccaggttc gggaagcgtt
     1441 tcagccagcc aatgctgtcg ggcctgagat cgtccggcag gtactcgttg taagcctcgt
     1501 ccagcaccac cagcacctcg cgcggcagcg cggcgatgaa gtcctcgaga tcgtgcgccc
     1561 gggccatcgt gccggtcgga ttattgggat tggcgatgaa cacgagccgg ctactcggcg
     1621 tgaccgcgcg cctcatcgcc gccagatcat gaccataatc ttttgccgcc acctctatgc
     1681 cggatgcgcc agccgactgc accgccagcg gatagacggc gaacgcgtgc tgcgaataga
     1741 gcgcatccag gccgggggcc agaaatgccc gagccgccat ctccagcacg tcgttcgaac
     1801 cgttgccgag aacgatgcat tccggcgcga cgccaagccg gcgtgaaagc gcctgcttga
     1861 gctcgaagcc gttgccgtcg ggatagcggg cgagatcggc aagcacggcc tcgatcgcgc
     1921 gctgcgcgag cgggctcacg ccgagcgggt tttcattgga agcgagcttg atgatgcccg
     1981 actcctcgag ccccatctcg cgcgcgagct ccgagatcgg cttgcccggc tggtacggcg
     2041 cgatcgcgcg gatgtagccg ggagcgaggt cgcaaatatc catgaataca gtgttgaatt
     2101 ttgagtgtta agtgttgagt tgactaccga tgcgataacg cgggttctct aacttaacac
     2161 tcaaaactaa gcactcaaca ctccgtcagg cgacagccgc aggataagag ccgaggttct
     2221 tcaggtacga cgccttctgc ttcaactcag cgagggcgag cgcgacgttg cggtcccgct
     2281 gatggccttc gacgtccacg tagaacacgt attcccacat cccggcgcgc gaaggtcggg
     2341 attcgagccg ggtcatgctc acctggttgg ccgccagcgg agtcagcagt tcgtgtattg
     2401 cccccggcac gttgcgagtg gagagaatga gcgaagtctt gtccttgccc gacggcgccg
     2461 cgtcgtgctg ggcgacgaca agaaagcgtg tggtgttctt cgcgtcgtct tcaatgttgc
     2521 gcgcaatcag cttcagaccg tagagttcgg ccgccatccg gggtccgacc gccgctgcgc
     2581 gccggtcgcg cgcggccagc cttgcagctt ccgcgttgct gacgaccgcc tcgcgctcgg
     2641 cgcgtggcag actgcgagcc agccactgct ggcactgggc gaggctctgg gtgtgcgaga
     2701 gtaccttgcg gacgtcggac agcttcccgc tcctgctcat caggcattga cggatcggca
     2761 gcatcacctc gccgcagacc cgggccggcg tcgtcaagag cagatcgagc gtgcgcccga
     2821 ttgcgccttc ggtcgaattc tccaccggca ccacgccgta gccggcggcg cccgtttcaa
     2881 cctggcggaa cacctcgtcg atggacgggc acgatgacag aacggcggcg cctccgaaat
     2941 gcttgaccgc ggcttcctgg ctgaaggtgc cctgcgggcc gagataggcc accctcatgg
     3001 agtcctccag cgcgcggcac gcggacatga tctccgcgaa caggcgagcg agcgcggcat
     3061 ccggcaatgg cccgcggttg ccggccagca cgccgcgcaa gacctgcgcc tcgcgctcgg
     3121 gacggtaggc cgcactttcc ttgagcttgc cgatgcgctg cgccaggcgc gcgcgcgccg
     3181 acagcagctt cagcaggcgc tggtcgagga cgtcgatgcg ggcgcggatc tgtttgagac
     3241 tactggtcat ggaaaggatt gtaaccgccg gggaacgcag gtgaaaccgg gccgctcccc
     3301 ggccggtttg cctcgtgtcg cccgtcgatg ccgcccgtag atgcttattg ggactcgacc
     3361 ggcaggtagc ggaccatcag cacgccctcg atgtcgccga gctgctcgat cagcttggcg
     3421 ggaaccttgc tgtcgacatc caccagcgtg taggccattt cgcccttgga cttgttcagc
     3481 atgttgtgga tgttgagcct ggctttcgcc atcgccgtcg agatctgccc taccatgttc
     3541 ggcacattgg cgttggcaat gccgacccgg tacggcgctt cgcgcgccat cgtcacctcg
     3601 gggaaattca ccgcgttgac gatgttgccg ttctcgagaa aatcccgcac ctggtccacg
     3661 accatgacgg cgcaattatc ctcggcctcg cgggtggagg cgccgagatg cggcagcgcg
     3721 atcacctggg gatgcccggc cagtttccgc gctggaaaat cgcaaacgta gtagcgcagg
     3781 cgcttcgccg cgagcgcttc cagtaccgct tcgttgtcca cgatgccctc gcgcgaaaaa
     3841 ttgagcagga tcgcgccact cttcatgagc ttgatgcggg cggcgtttaa cagattgcgg
     3901 gtcacatcga gcagcggcac gtgtatcgtg acgaaatcgg atgccttcag cacctcctcg
     3961 atactgtggg cttttttgac ctgggcaggc aatccccagg ccgcgtctac ggtgatctcg
     4021 ggatcgtagc cgaggacatt catgccgagc ctgatggcag cgtccgccac caggctgccg
     4081 accttgccca ggccgatcac gccgagcgtg tggcccggga gctccactcc ggcaaagtcc
     4141 tttttgccct cctcgaccag cttgtgcagt gtcttgtcgt ctcccttcag cccgtccacg
     4201 aaacgcaatg ccggcaccag gttgcgcgac gcgacgagca gccctgcgat caccagctct
     4261 ttcacggcat tcgcattggc accgggcgcg ttgaacaccg gcacgccgcg cttgctcatc
     4321 ttgtcgaccg ggatgttgtt cacgccggcg ccggcgcggc cgatcgcctt tacactcggg
     4381 ccgatctcca tcggatgcat gtcgtgcgat cgcacgagaa ttgcgtccgg atccgcaatc
     4441 gaattgccgc acgtgtagcg gccggaaggc aggcgcttca gccccaggct cgaaatcgag
     4501 ttcagcgtga gtatcttgta tggttcagcc atgtttgcgc tcgaactcct tcatgaattc
     4561 caccagcgcc ttcacgcctt ccaccggcat ggcgttgtag atcgaagcgc gcatgccgcc
     4621 caccgcccgg tgacccttca attcgctcag gccgcaggcc ctggcctgct ggaggaattc
     4681 ctcgtcaagc gtgtcgctgc gcaggctgaa cgggatattc atgcgcgaac ggtcctcctt
     4741 cgccaccgga gagcgataga actcggtctg gtcgagatag tcgtaaacga gctgcgcctt
     4801 cgcgatattg atttcctcca tcttcttcac gccgccctgc ttcttcagcc actggaagac
     4861 cagcccggcc atatagacgg agtaggtcgg cggggtgttg tgcatcgagt ccgccccggc
     4921 ctggaccttg tagtcgaaca cggacggcgt cgccggcatc gcctggccga tcaggtcatc
     4981 gcgcacgatc acgatcgcca ttcccgccgg tccgatgttc ttctgggcac cggcgtagat
     5041 caatccgaaa cggctgacgt cgagcgggcg tgaaaggata tgcgaggagg cgtcggcgac
     5101 gagcgggata tcgcccgtat ccggtgtcca atgaaactcc acgccgccga tcgtctcgtt
     5161 cgaggtgtag tgcacgtacg ccgccgcggg atcgaccttc cacttgtcct gcctgggcgc
     5221 gtaggtgaag ctggcgtcct ccgaactcgc ggcgatgctt accttgcagt aacgcttcgc
     5281 ttcggcgatc gcccgggtcg accagtggcc ggtgtttacg tagctggcgg tggtcctgcc
     5341 ccggagcagg ttcatcggca cgatcgaaaa ctgccccgcg cccccgcctt gcaggaacag
     5401 cactttgtaa ttcttcggga tcgccagcag ttcgcgcaga tcggcctcgg cctgcgcatg
     5461 gatgccaacg aacgccttgc cgcggtggct catttccatc acggacattc cgctgccgtg
     5521 ccagtcgaga agttcctcgg ccgccagttt cagcaccggt tccggcagcg tcgccgggcc
     5581 tgctccaaaa ttgaataccc tggccatgta attcgcttcg atttgggaaa ttgccgttgg
     5641 aagaatgcca agcttcgatt gcagcgcgcg acattgcgcg ggaagatgac cgctattgac
     5701 ccgccttcgc ggcgcccggg gactcgctct cgccgttttc atccgattcg gcgatcttct
     5761 gcagaccggc cagtttttcg ccttcgtcga ggttgatgag ccgcacgccc tgggtggatc
     5821 ggcccatttc gcgcacctcc gagactcgcg tgcggattaa cacgccgccg gtggtgatca
     5881 gcatgatctc atcctcggga gcaaccagtt ccgcggcgag cagcttgccg gtcctctcgc
     5941 tgcaatcgat tgcgatgatg ccctgggtgc cgcgcccgtg ccgggtatac tcggatacaa
     6001 gcgtgcgctt gccatagccg ttctcggtgg ccgtcagcac cgaaagctgt tcgtcgctgg
     6061 atatgagcag tgacaccacg tgccggtccc tggcaagctt catgccgcgc acgcccgccg
     6121 cgccgcggcc catggcacgc acttcgtcct cggcaaagcg caccgccttg ccctcgtcgg
     6181 agaagagcat gacatcctgc ttcccatccg tgagcgagac cccgatcaga tagtcgccgt
     6241 catcgagcgt taccgcgatg atgcccttgg ccatcgggcg cgagaacgcc gacagcgcgg
     6301 ttttcttgac ggttcccatc gccgtggcca tgaacacgaa atgctcgtcg tcgaactggg
     6361 aaaccggcag cacggcggtg atcttctcgt gctccatcag cggcaccagg ttcacgatcg
     6421 gcttgccgcg ggaagcgcgg ct
//
LOCUS       DBNGSG010000341         2498 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3167859, whole genome shotgun sequence.
ACCESSION   DBNGSG010000341 DBNGSG010000000
VERSION     DBNGSG010000341.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3167859"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1183
                     /locus_tag="AC158Q_08895"
     CDS             <1..1183
                     /locus_tag="AC158Q_08895"
                     /inference="COORDINATES: protein motif:HMM:NF012678.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HIR3339932.1"
                     /translation="ATGYPFRLYSGKDALERLPDEAARLRASRAFIVCGRTVSRKTPL
                     ISRMRQLLGERLAGVFDEIDKDTSRSSVTRAAEAARAAGTDLVIGVGAGSAIQGARIV
                     TILLAEKRPVEELVTQYPEHGPAVSPRLMEPKLPIINVLTAPTTAQNRAGSRMKDDAT
                     ARGMEFFDPKTRPAAIFWDTDALLTAPPSLIRTGAGTIYCRAVMNLGAVAENPLVEGD
                     RLQSYRLAAGALPRIADATDPAPRFDLCAATLLQNREADDGGSQFARHWAMRVAYAFS
                     TSIISVFPHVGQGEAYTALMGTMLRRLGDRDPEPMARIAAGLGVSGGSPPDIPGRIAE
                     RMEKEFAALGMPTRLSGLGIERGRLPQVLELSLRNFNADPKRDFVKERERLGEILEAA
                     W"
     gene            1270..1656
                     /locus_tag="AC158Q_08900"
     CDS             1270..1656
                     /locus_tag="AC158Q_08900"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF046335.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HIR3339933.1"
                     /translation="METLKVGMKHTQEWVVEEKHTTQRGDYRVFSTPSMTQLVESTAH
                     QLAAPHLKPGQGQVGILVTIRHMGPTPMGGKVRAEAELVAIDRRKLSFRVTVHDELEQ
                     VGEAEHDRFVIDLDKYMDKLKKKIGA"
     gene            1703..2305
                     /locus_tag="AC158Q_08905"
     CDS             1703..2305
                     /locus_tag="AC158Q_08905"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3339934.1"
                     /translation="MNAPQSLKFGHFGINCFDIARMEDFYTRVIGMVVADRGYVPAPA
                     DRHIVFMTLDPGEHHQLILCSGRTEGRIEKGPFRGGGRGSAINQISFHCASLGELRKV
                     QARLAAAGLTDGTPINHGNAWSIYIRDIEGNPMEFYADSPWYTPQPCGEPFDLSLSDE
                     EIHRRTEAMCRARPGFEPAESWRARLAVRIRTALDEVGGR"
BASE COUNT          468 a          824 c          854 g          352 t
ORIGIN      
        1 cgcgacgggc tacccgttcc ggctgtacag cggcaaggac gcactcgagc ggctgccgga
       61 tgaagccgcg cgtctgcggg cgagccgcgc cttcatcgtg tgcgggcgca cggtatcgcg
      121 caagacaccg ctgatatcgc gcatgaggca gctcctcggc gagcgtcttg ccggtgtatt
      181 cgacgaaatc gacaaggaca cctcgcgctc ctcggtcacg cgcgcggcgg aggctgcccg
      241 cgcggcaggg acggacctcg tgatcggcgt gggcgcgggc agcgcgatcc agggcgcgcg
      301 catcgtgacg atcctgctgg cggagaaacg gccggtcgag gagctcgtga cgcaataccc
      361 cgagcacggc cccgcggtga gccccaggct gatggagccc aagctcccga tcatcaatgt
      421 gttgaccgcg cccaccacgg cgcagaaccg cgcgggctcc cggatgaagg acgacgccac
      481 cgcccgcggc atggagttct tcgatcccaa gacgcgtccc gccgcgattt tctgggacac
      541 cgatgcgctg ctgactgcac cgccgtcgct gatccgtacc ggcgcgggga ccatttactg
      601 ccgggcggtg atgaacctcg gcgctgtcgc ggagaatccg ctggtcgaag gcgaccggct
      661 gcagtcctac cgcctcgcgg ccggcgccct gccgcgcatt gccgatgcca cggatcccgc
      721 gccgcgcttc gacctgtgcg cggcgacgct gctgcagaac cgggaagccg acgacggcgg
      781 ctcccagttt gcccggcact gggcgatgcg ggtcgcctac gcgttctcga cctccatcat
      841 cagcgtgttt ccccacgtcg ggcagggcga ggcgtacacc gcgctcatgg gcacgatgct
      901 gcgccggctc ggcgatcgcg acccggaacc gatggctcgc atcgcggcgg gtctcggggt
      961 ctcaggtggc tcgccgccgg atatcccggg ccggatcgca gagcgaatgg aaaaagagtt
     1021 cgccgccctg ggcatgccga cgcgcctgtc cgggctcggc atcgagcgcg ggcggctgcc
     1081 gcaggtgctg gagctctcgc tcaggaactt caatgccgac ccgaagcgtg atttcgtgaa
     1141 ggagcgcgag cggctgggcg aaatactgga agcggcctgg tagaaaagcc ggaaaccgcc
     1201 gatgaacgcc gatgcacgcc gatagaatga aacatcggca agccgctgcg gacagattcg
     1261 aggaccccta tggaaacgct gaaggtcgga atgaagcaca cgcaggaatg ggtggtggag
     1321 gaaaagcaca ccacccagcg cggggactac cgggtattct cgacgccctc gatgacgcag
     1381 ctcgtcgagt ccaccgcgca ccagctcgcc gcgccgcact tgaagccggg ccaggggcag
     1441 gtcgggatcc tggtcaccat acgccacatg gggccgacgc cgatgggcgg gaaagtgcgc
     1501 gcggaggccg agctcgtcgc catcgaccgg cgcaagctct ccttcagggt gacggtccac
     1561 gacgagctcg agcaggtggg cgaggccgag cacgaccgct tcgtgatcga cctcgacaag
     1621 tacatggaca agctgaagaa gaagattggc gcgtgaggcc gggcctataa tcgggtctcc
     1681 gccggaaccg aggagacacg gcatgaacgc cccgcaatcc ctgaagttcg gccacttcgg
     1741 catcaactgc ttcgacatcg cgcgcatgga ggacttctac acgcgcgtaa taggcatggt
     1801 cgtggccgac cgcggctacg tgcccgcgcc ggccgaccgg cacatcgtct tcatgacgct
     1861 cgacccgggc gagcatcacc agctgatcct ctgctcgggg cgcaccgagg gcaggatcga
     1921 gaaggggccg ttccggggcg gcgggcgcgg ctcggccatc aaccagatct cgtttcactg
     1981 cgccagtctc ggcgagctgc gcaaggtgca ggcgcggctg gcggcggcgg ggctcaccga
     2041 cggcacgccg atcaaccacg gcaacgcgtg gtcgatctac attcgcgaca tcgagggcaa
     2101 cccgatggaa ttctatgcgg acagcccgtg gtacacgccc cagccctgcg gcgagccgtt
     2161 cgatctttcc ctgagcgacg aggaaatcca ccgccgcacc gaggcgatgt gccgcgcgcg
     2221 gcccggcttc gagcccgccg agtcctggcg cgcccgcctg gccgtcagga tacggacggc
     2281 gctcgacgaa gtcggcggtc gctgaggcag gattgagccg ccgagacgcc gagagaaaca
     2341 agaacaagaa ttagccgccg atgaacgccg ataaacgccg atagaacaaa agcagaaaca
     2401 gaattaacgt cgtgctctgc ggcgggccgc agcgcgatga ttgataaaga gcgaacgtct
     2461 cgcggcccgt ccgcagcagc tacgggttag aaggccta
//
LOCUS       DBNGSG010000342         3912 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1299136, whole genome shotgun sequence.
ACCESSION   DBNGSG010000342 DBNGSG010000000
VERSION     DBNGSG010000342.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1299136"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..1489)
                     /locus_tag="AC158Q_08910"
     CDS             complement(35..1489)
                     /locus_tag="AC158Q_08910"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HIR3339935.1"
                     /translation="MQPAPLTRIATLLLALALANCAQNPVTGNPNLVLLSESQEIAIG
                     RREDTNVRKQYSAYDDARLQQYVDGIGQRLARAGHRPGLEYRFLVVDSPEVNAFALPG
                     GYIYITRGILAYLGSEAELAAVLGHELGHVTARHSVQQLTAATAANIGANILQIFVPQ
                     ARGALGGNAINILGGALLSGYGREHELEADRLGAEYLARTGYDPQAMVKVVGVLKNQE
                     LFDAEVAKAEGRQPRAYHGLFASHPDNDTRLREMVGEAARFKQESIRVAQEEFLRRSD
                     RMIYGDGPKQGIVRNNDFYHEELGLAMSFPAGWRVQNRPRNVAATSPDGGEALIDLRA
                     AGPAKGAPADVLREALKLSADEEPTPTTISGLQAATASTTIGGRPTRAACVFHRGTAY
                     LIGAQARTAAGFQQYQEAIQASILSIRALTEKERAFARPLRLRVITAQADTTFAELAR
                     RSPLGRFAEGHLRVINGLYPAGEPVAGQPLKIIE"
     gene            1727..3094
                     /locus_tag="AC158Q_08915"
     CDS             1727..3094
                     /locus_tag="AC158Q_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR3339936.1"
                     /translation="MSAFKDPFNPEVKLGCGCGRHGDQAAHDADLARSSSDTDALASR
                     AVDQAVMRALFPNDGTRRRFLKAVGANSAMAAIAQFFPLAMAREAFAQGRGEIEKPNL
                     KVGFIPITCATPIIMSQPMGYFGRAGLNVDVVKTAGWAVIRDKTLNKEYDAAHMLSPM
                     PLAITLGVGSRPIPYTMPAVENINGQAITLAIKHKDRRDPRSWKGFKFAVPFDYSMHN
                     YLLRYFVAEHGLDPDRDIQIRAVPPPEMVANLRADNIDGFLAPDPVNQRAVYDGVGYI
                     HTLTKDMWEGHPCCAFAASREFVTKLPNTYRALLKAIMEATAFAARPENRKQIAEAIA
                     PANYLNQPVTVIEQVLTGTYADGLGNVKKVPNRIDFDPFPWHSFAVWIMTQMKRWGQL
                     KGDVDYAKVAREVFLATDAVKLMKEAGMKPPATTSKKFAVMGKEFDPARPDAYIASFA
                     IKRTA"
     gene            3127..>3912
                     /locus_tag="AC158Q_08920"
     CDS             3127..>3912
                     /locus_tag="AC158Q_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3339937.1"
                     /translation="MKGLQFWGAPLLSVAILLAFLAVWHAGTRQEAGAQVVDAEYAKL
                     VGAEAATGKKSAFPTPAEVGRKIWGHIADPFYDKGPNDKGVGIQLAYSIARVLTGYLF
                     AFLVAVPLGFIIGMSPLFSRALDPFIQVLKPISPLAWMPLALYTIKDSSISGIFVIFI
                     CSVWPMLINTAFGVATVRRDWLNVAKTLELGTWRTAFQVILPAAAPTILTGMRISIGI
                     AWLVIVAAEMLVGGTGIGYYVWNEWNNLDLSAVIFSILMIGLVG"
BASE COUNT          676 a         1261 c         1291 g          684 t
ORIGIN      
        1 aaattccaac gagaaacgag aaacgagaaa cgagtcactc tatgattttc aaaggctgtc
       61 ccgccaccgg ttcacccgcg ggatacaggc cgttgatcac tcgcaggtgt ccttcggcga
      121 aacgccccag cggcgaacgg cgcgccagtt ccgcgaacgt cgtgtccgcc tgcgctgtga
      181 ttacccgcag ccgcagcggg cgcgcgaacg cccgttcctt ctcggtcagt gcgcgaatac
      241 tcaggatcga ggcctggatc gcctcctggt actgctggaa tcccgcggcg gtgcgcgcct
      301 gcgcgccgat gaggtaggcg gtgccgcggt gaaacacgca ggcggcacgg gtggggcggc
      361 caccgatcgt agtgctggcg gtcgcagcct gcagtccgct gatagtggtt ggcgtcggtt
      421 cctcgtcggc cgacagcttg agcgcctcgc gcagcacgtc ggcgggcgcg cccttggcgg
      481 gcccggccgc gcgcagatcg atcagcgcct cgccgccgtc gggactggtg gcggcaacgt
      541 tccgcggtct gttctgcacg cgccagcccg cgggaaagct catcgcgagc ccgagctcct
      601 cgtgatagaa gtcgttgttg cggacgatgc cctgcttcgg gccgtcgccg tagatcatgc
      661 ggtcggaacg gcgcaggaac tcctcctgcg cgacgcggat cgattcctgc ttgaagcggg
      721 ccgcctcgcc gaccatctcg cgcagacggg tgtcgttgtc ggggtgcgaa gcgaacagcc
      781 cgtggtaggc gcgcggctgg cgcccctcgg ccttcgccac ttccgcatca aacagctcct
      841 ggttcttcag cacgccgacc accttcacca tggcctgcgg gtcgtagccg gtgcgcgcga
      901 ggtactcggc gccgaggcgg tcggcttcca gttcgtgctc gcgcccgtag cccgagagca
      961 gcgcgccgcc gaggatgttg attgcattgc cgcccagcgc cccgcgtgcc tgcggcacga
     1021 agatctgcag gatgttcgcg ccgatgttgg cggcggtggc cgcggtcaat tgctgcaccg
     1081 agtggcgcgc ggtgacgtgc ccgagctcgt gcccgagcac cgccgcgagc tcggcctcgg
     1141 aaccgagata ggcgaggatg ccgcgcgtga tgtagatata gccgccgggc agcgcgaacg
     1201 cgttcacttc gggcgaatcc accaccagga agcggtactc gaggccgggg cgatggcccg
     1261 cccgggcgag ccgctggccg atgccgtcca cgtactgctg cagacgggcg tcgtcatagg
     1321 cgctgtactg cttgcggacg ttcgtgtcct cgcgccggcc gatcgcgatc tcctgcgact
     1381 ccgagagcag caccaggttg gggttgccgg tgacggggtt ctgcgcgcag ttggcgagcg
     1441 cgagcgcgag gaggagcgtc gcgatgcgcg tgagcggagc cggttgcatg ggtggatttt
     1501 acttccaata aagcgaagca cgaatctggt gcgcatcgca ctaataacgt gcatgttctc
     1561 cgccggtgtg ctggcggaag tcttcatgag gcgattcggg cggccatcga taatcgagac
     1621 aaatcatatc gttgacgcct gatattttcg attttcggcg gatcgcgatt caacctggta
     1681 tgcatcctgc aatcaggcct gtcatgactc tcatcaggag gccgtgatgt ccgcgttcaa
     1741 agacccgttc aatcccgaag taaagctcgg ctgcggttgc ggccgtcacg gcgaccaggc
     1801 cgcgcacgac gccgatctcg cgcgctcgag cagcgacacc gacgcgctcg cgagccgcgc
     1861 cgtggaccag gcggtgatgc gcgccttatt ccccaacgac gggacgcgcc gccgtttcct
     1921 gaaagcggta ggcgcgaact cggcgatggc ggcgatcgcc cagttctttc cccttgccat
     1981 ggcgcgcgag gcgttcgcgc agggccgggg tgaaatcgag aagcccaacc tcaaggtcgg
     2041 attcatcccc attacctgcg cgacccccat catcatgtcg cagccgatgg gctacttcgg
     2101 gcgcgcgggc ctgaacgtgg acgtggtgaa gaccgccggc tgggcggtga ttcgcgacaa
     2161 gacgctcaac aaggagtacg acgccgcgca catgctctcg ccgatgccgc tcgcgatcac
     2221 gctcggcgtc ggttcgcgcc cgatcccgta caccatgccg gccgtggaga acatcaacgg
     2281 ccaggcgatc acgctggcga tcaagcacaa ggacaggcgc gatcccagaa gctggaaggg
     2341 cttcaagttc gcggtgccgt tcgattactc gatgcacaac tacctgctgc gctatttcgt
     2401 ggcggagcac gggctcgacc cggaccgcga catccagatc cgcgcggtgc cgccgcccga
     2461 aatggtcgcc aacctgcgcg ccgataacat cgacggcttc ctcgcgccgg accccgtgaa
     2521 ccagcgcgca gtctacgacg gcgtcggcta catccacacg ctgaccaagg acatgtggga
     2581 agggcatccg tgctgcgcat tcgccgcgag cagggagttc gtgacgaagc tgcccaatac
     2641 ctatcgcgcg ctgctgaagg cgatcatgga ggcgaccgcg ttcgccgcca ggcccgagaa
     2701 ccgcaagcag atcgcggagg cgatcgcgcc ggcgaactac ctcaaccagc cggtcacggt
     2761 gatcgagcag gtgctgaccg gcacctatgc cgacgggctc ggcaacgtga aaaaggtgcc
     2821 gaatcgcatc gacttcgacc cgttcccgtg gcactcgttc gcagtctgga tcatgaccca
     2881 gatgaagcgc tggggccagc tgaagggcga cgtggactac gcgaaggtgg cgcgcgaggt
     2941 gttcctggcc accgacgccg tgaagctgat gaaggaagcg ggcatgaagc cgccggcgac
     3001 caccagcaag aagttcgcgg tgatgggcaa ggagttcgat cccgccaggc ccgatgccta
     3061 catcgccagc tttgccatca agcgaacagc gtgaaaggtg attcgtgatt ggtgattggt
     3121 gaaagcatga aaggactgca attctggggc gcgccgctgc tttcggtcgc gatcctgctc
     3181 gccttcctcg cagtgtggca cgcgggcacg cggcaggagg cgggcgcgca ggtggtggac
     3241 gccgagtacg cgaagctggt cggcgcggaa gccgccaccg ggaagaaatc ggcgttcccg
     3301 acaccggccg aggtgggacg caagatctgg gggcacatcg cggatccttt ctacgacaaa
     3361 ggtcccaacg acaagggcgt cggcatccag ctcgcgtact cgatcgcgcg cgtgctcacg
     3421 ggctacctgt tcgctttcct ggtcgcggtg ccgctcggct ttatcatcgg catgtcgccg
     3481 ctcttcagtc gagcactcga tcccttcatt caggtgctga agccgatctc accactcgcg
     3541 tggatgccgc tcgcgctcta cacgatcaag gactcgtcga tcagcggcat cttcgtgatc
     3601 ttcatctgct ctgtgtggcc gatgctgatc aatacagcat tcggcgttgc gaccgtgcgc
     3661 cgcgactggc tgaatgtcgc caagacgctc gagctcggca cttggcgcac ggccttccag
     3721 gtcatcctgc cggccgccgc accgacgatc ctcacgggca tgcgcatttc gatcggcatt
     3781 gcctggctcg tgatcgtcgc cgccgagatg ctcgtgggtg ggacgggcat cggctactac
     3841 gtctggaacg agtggaacaa cctggacctg tccgcggtca tcttctccat cctgatgatc
     3901 ggcctcgtcg ga
//
LOCUS       DBNGSG010000343        11183 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_790806, whole genome shotgun sequence.
ACCESSION   DBNGSG010000343 DBNGSG010000000
VERSION     DBNGSG010000343.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 11183)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11183)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11183
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_790806"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /gene="phaR"
                     /locus_tag="AC158Q_08925"
     CDS             complement(<1..547)
                     /gene="phaR"
                     /locus_tag="AC158Q_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HIR3339938.1"
                     /translation="MTQQPRIIKKYPNRRLYDTETSSYITLADVKKLVLEQVAFKVED
                     AKTHEDLTRSILLQIILDEETAGAPMFSSDMLSQIIRFYGNAMQGMMGSYLEKNIQTF
                     IEMQKRLQDQSRQIYGDNPLLSADAWSEFIKMQGPAMQGLMGRYLEQSANAFMEMQQQ
                     LQQQTRNLFGNFPFPNFTGGGP"
     gene            complement(746..1489)
                     /gene="phbB"
                     /locus_tag="AC158Q_08930"
     CDS             complement(746..1489)
                     /gene="phbB"
                     /locus_tag="AC158Q_08930"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011657937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HIR3339939.1"
                     /translation="MAQRVAVVTGGMGGLGEAICVKLAQMEFKVVATYSPGNTKSADW
                     VKDMKSRGFEILAVPVDVSDFDSCQKAAARIHSETGPIDVLVNNAGITRDMTFKRMGK
                     VDWDAVLRTNLDSVFNMTKPVCDGMVDRGWGRIINISSINGQKGAFGQTNYSAAKAGM
                     HGFTKALALEVARKGVTVNTISPGYIGTKMVLAVPKEVLDSKIIPLIPVGRLGKPDEV
                     AGLVAYLCSDEAAFVTGANIAINGGQHMQ"
     gene            complement(1532..3271)
                     /locus_tag="AC158Q_08935"
     CDS             complement(1532..3271)
                     /locus_tag="AC158Q_08935"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHA/PHB synthase family protein"
                     /protein_id="HIR3339940.1"
                     /translation="MERPADNADLMRSCIEMLNRQLVNGVAKPLDLDAGFDIAAVLRS
                     LTESVTQDAGRWLEIQNRYYRKRLELWAAYAQPQPGKPAQPLIEPDSSDRRFRAPEWR
                     KEPYFDYLAQSYLLTAQWLREVVDAARTDPRTKRKLDFYARQFVDAMSPANFPWSNPE
                     AIRLAAETEGESLAKGLRNLAADLDRGMISMTDETAFEVGRNLAITPGAVIYQNDFMQ
                     LIQYRPATATVFERPLLMVPPCINKYYILDLQPENSFVRYAVERGHTVFMVSWRNMPP
                     AMGRATWDDYLEQGVIKALAVAGEICGAGQVNALGFCVGGTLLGSALAVLRARREDRA
                     ASLTLLATMLDFHDTGELSVFVDDAYVAKREFDFAEGGLLPGRELALTFASLRANDLI
                     WHYVVNNYLKGRTPEPFDLLYWNADSTNLPGAMYRYYIRNMYLENNLRVPGKLTMCGV
                     PVDLGKVDMPAYVLATREDHIVPWRTAYSSARLLGGRIEFVLSASGHIAGVINPASKN
                     RRNFWLNPQREEDPGKWLAAATPQPGSWWQHWADWLAPFGGAQVAARAEPGGRNYREI
                     EPAPGRYVKEKCS"
     gene            complement(3398..4180)
                     /locus_tag="AC158Q_08940"
     CDS             complement(3398..4180)
                     /locus_tag="AC158Q_08940"
                     /inference="COORDINATES: protein motif:HMM:NF014582.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIR3339941.1"
                     /translation="MTLAWILAATVAGGVLSVVAAAAVAFNARASWVPILISYAVGAL
                     LGAVFLEILPHAFGTTDSLENLAATILAGILLFFVLEKLVLWRHCHVEQCEAHDPQAA
                     PNHNHDHGRSGMMIMIGDTFHNFVDGILIAAAFLANHELGIVTAIAIIAHEVPQEVGD
                     FLILLHSGYSKSRAMFFNLLSSAAMVAGGVLAYYALQSLQQWIPTLLGLAAASMLYVS
                     VADLIPGLHRRPELQATLQQVVLIALGVLTIWLVGDIAHTHA"
     gene            complement(4335..5075)
                     /gene="pgeF"
                     /locus_tag="AC158Q_08945"
     CDS             complement(4335..5075)
                     /gene="pgeF"
                     /locus_tag="AC158Q_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HIR3339942.1"
                     /translation="MKSLHPDWIVPDWPAPANVKAFITTRAGGISGGAYASFNLGLRT
                     DDSPQAIAANRQRLRAALPQEPKWLRQVHGSLVVEADDLNGVPEADAAVARRPRTVCA
                     VLVADCIPVLLADRAGATVGIAHAGWRGLAGGVIENAVERMTVAAEELIAYLGPGIGR
                     HAFEVGADVREAFLARDARAEAAFAPHTPGKWLADLFLLARQRLAGAGIRSIHGGELC
                     TYSDPQRFYSYRRDRATGRMAAVIWRTD"
     gene            complement(5072..6031)
                     /locus_tag="AC158Q_08950"
     CDS             complement(5072..6031)
                     /locus_tag="AC158Q_08950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR3339943.1"
                     /translation="MSPVELVVPAGCAGLRLDQALANLVPEYSRSRLAQWIKAGRVTL
                     EGRAAPPRQKVREGERIRVIPTPDPATTVHRAEHMALDIVYEDDALLVVNKPAGLVVH
                     PGSGNWQGTLLNALLGRAPALAGIPRAGIVHRLDKDTSGLLVVAKTLAAQTDLVRQLK
                     ARSVKREYLALVQGRVARDGRIDAPIGRQPLKRTRMAVTARGRQAVTHYQVLERYERA
                     TLLRCRLETGRTHQIRVHLASLRHPLIGDPAYGKPGGAIPFPRQALHAERLGLLHPRT
                     RKPMIWQADPPQDMHELIAALRCGEIPPGRESRITNHESRRIK"
     gene            6072..6872
                     /locus_tag="AC158Q_08955"
     CDS             6072..6872
                     /locus_tag="AC158Q_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HIR3339944.1"
                     /translation="MLIVRHSLALLLGVLIGACGLLPEEQDETKGWSAQKIYAEAKDN
                     LDKANYEQAIKYYQKLESRYPYGRFAQQAQIEIAYAYYKDSEPVQAVVAADRFIKLHP
                     NHPNVDYVYYLKGLANFNDDLGWTGFISGQDLSERDPKAAREAFDAFKELATRYPGSR
                     YTPDAIARMNYLLNALASHEVHVARYYMKRGAYVAAANRAQYALKTYPQAPANEEGLV
                     ILVRAYDALGMDDLRNDAERVLKKNFPDSVYLKGGPQGTAPWWQFWNW"
     gene            complement(6974..7834)
                     /locus_tag="AC158Q_08960"
     CDS             complement(6974..7834)
                     /locus_tag="AC158Q_08960"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR3339945.1"
                     /translation="MTAFALALILAAAVIHASWNYFLKRSGGGAVFVWLFASLSALIY
                     LPLAAFIIWWQKPEFGWEHYGMMLASAVLHTAYYLLLDRGYRSGDLSVVYPIARGTGP
                     LITVLCAVLLLGEDPSALAVAGALLIGGGAVALTGDPRKLRQSGNLHAVGFALLTGCM
                     IASYTLVDKIAVAAWLIPPLVQDWATNFGRVVLMTPMMLGRGHEIVPTWRRAKKEIIA
                     VALLCPLSYILVLTAMVFTPVSYVAPAREVSILVAALMGTHLLAEGNVAQRLAAAAAM
                     VAGIICLALG"
     gene            complement(8058..10034)
                     /locus_tag="AC158Q_08965"
     CDS             complement(8058..10034)
                     /locus_tag="AC158Q_08965"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HIR3339946.1"
                     /translation="MNPPADKPLWQPSPERVRNANLTAFARQARERWGIAANDFPGLH
                     RWSVTEPAQFWQSVWSFCNVIGDERDGPALEDGRRMPGARWFPDARLNFAENLLRRRD
                     RSVAIVFWGEDRLKTTVTCAELHSEVSRLSQALQAAGVKPGDRVAAYMPNVPGTVIAM
                     LAATSLGAIWSSCSPDFGVQGVLDRFGQIAPKILFAADGYFYNGKTIDLLPRLKEIAG
                     AIPSIEKLIVVPYTRPRPPLTGLPRAVNVHDLMAPWRAGDIRFERFPFDHPLFIMYSS
                     GTTGVPKCIVHGAGGTLIQHLKEHQLHVDLKPGDRLFYFTTCGWMMWNWLASALASRA
                     VIALYDGSPFMSGGRILFDYADAARITVLGTSAKFIDSAAKAGLKPRLTHRLTSVKTL
                     LSTGSPLVPEGFDYVYREVKSDLHLASISGGTDLIGCFVLGNPIAPVWRGEIQCAGLG
                     MKVEVYDEQGRPVRGAKGELICTVPFPSMPVGFWNDPDGAKYRAAYFEKFPGVWCHGD
                     YIEQTARGGFVIYGRSDAVLNPGGVRIGTAEIYRQVESLDEVIESLVIGQDWPPDRPA
                     DVRMVLFVRLREGLQLDDALIERIKRKIRDNTTPRHVPARIVQVPDIPRTKSGKIVEL
                     AVRNVVHGRPVRNVEALANPEALAHYKDRPELTS"
     gene            complement(10290..>11183)
                     /gene="lysS"
                     /locus_tag="AC158Q_08970"
     CDS             complement(10290..>11183)
                     /gene="lysS"
                     /locus_tag="AC158Q_08970"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010091959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HIR3339947.1"
                     /translation="TPMMQPIPGGAAARPFATHHNALDMDLYLRIAPELYLKRLVVGG
                     CERVFEINRNFRNEGISTRHNPEFTMLEFYAAYTDYEYMMAVIPRMLAHAAQAALGAT
                     RVTYQGSEIDFARPYERLTIRQAIRKYHPGITDAILNDRAALIAALKQRNIAYREGDG
                     IGGLQLTLFENTVEDQLVQPTFIVDYPTEVSPLARRSDRNPEITERFELYIAGREIAN
                     GFSELNDPEDQAERFTQQARQKEAGDAEAMHFDADYIRALEYGMPPASGAGIGIDRLV
                     MLLTDSPSIRDVILFPLLRPE"
BASE COUNT         1977 a         3504 c         3689 g         2013 t
ORIGIN      
        1 ggggcccgcc gccggtgaaa ttggggaagg ggaagttgcc gaagagattg cgggtctgct
       61 gctgcagctg ctgctgcatt tccatgaagg cgttggcgct ctgctcgagg tagcggccca
      121 tcagcccctg catcgccgga ccctgcatct tgatgaattc gctccacgca tccgcgctga
      181 gcagcgggtt gtcgccgtag atctgccgcg actggtcctg caggcgcttc tgcatctcga
      241 tgaaggtctg gatattcttc tcgagatagc tgcccatcat gccctgcatg gcattgccgt
      301 agaagcggat gatctgcgag agcatatcgc tcgagaacat cggcgcccct gcggtttcct
      361 cgtccaggat gatctgcagc aggatgctgc gggtcaggtc ctcgtgggtc ttggcatcct
      421 ccaccttgaa ggcgacctgc tccagcacca gcttcttgac gtccgcaagc gtgatgtaac
      481 tgcttgtttc ggtgtcgtaa agtcgccgat tcgggtactt cttgatgatc cggggctgct
      541 gcgtcatgaa gtccgttgtg ctttaccggg gtgcatcatt atttcaccaa gagggggcga
      601 atgcaaggct aaaggattga aaaatatcgg taaatcgatg atgccgtgta tgtgatgctt
      661 cgcatcatga cggtcgtgcg cggcattgat tcatggcgga gaaaataatc ggtcaggaat
      721 ttgcgcgggc gcagcctgca aattcctatt gcatgtgctg gccgccgttg atcgcgatgt
      781 tggcgccggt gacgaacgcg gcttcatccg agcacaggta ggcgaccagt cccgccactt
      841 cgtccggttt gccaagcctg cccaccggga tcaacgggat gatcttgctg tccagaacct
      901 ccttgggcac cgccagtacc atcttggtgc cgatgtagcc gggagaaatg gtgttgaccg
      961 tcacgccctt gcgtgccact tccagcgcga gcgccttggt gaagccgtgc atgccggcct
     1021 tggcggcgga gtagttggtt tgcccgaacg cgcccttctg gccgtttatc gatgaaatgt
     1081 tgatgatgcg tccccagccc cggtccacca tgccgtcgca cacgggcttg gtcatgttga
     1141 acacgctgtc gagattggtg cgcagcaccg cgtcccagtc caccttgccc attctcttga
     1201 acgtcatgtc ccgggtgatg cccgcgttgt tgacgagcac atcgatgggg ccggtctcgg
     1261 agtgaatcct cgccgcggcc ttctggcacg agtcgaagtc cgacacgtcc acggggacgg
     1321 ccagaatctc gaagccccgg gacttcatgt ccttcaccca gtcggcggac ttggtgttgc
     1381 cgggcgaata ggtcgccacg accttgaatt ccatctgggc gagcttgacg cagattgcct
     1441 cgcccaggcc gcccatgccg ccggtcacga ccgccactcg ttgtgccatg tgaacgcctc
     1501 ctctgactgg tcaaacccga tgatcgtcac atcaactgca tttttccttg acgtaacggc
     1561 ccggggcggg ctcgatctcc cgataattcc tgcctcccgg ctccgcgcgc gcggcaacct
     1621 gcgcgccgcc aaaaggcgca agccagtcgg cccagtgctg ccaccagctg cccggctgcg
     1681 gcgtcgccgc ggcaagccat ttcccgggat cctcctcccg ctgcggattc agccagaaat
     1741 tgcggcggtt cttcgaagcc gggttgatga ccccggcgat atgcccgctc gcggacagca
     1801 cgaattcgat tctaccccca agcagccggg cgctggagta ggcggtgcgc catggcacga
     1861 tatggtcttc ccgggttgcg agcacgtacg ccggcatgtc caccttgccc aggtccacgg
     1921 gcacgccgca catggtgagc ttcccgggga cccgaagatt gttctcgaga tacatgttgc
     1981 ggatgtaata gcggtacatc gcgccgggca ggttggtgct gtcggcgttc cagtagagca
     2041 ggtcgaacgg ctcgggcgtg cggcccttga gatagttgtt caccacgtaa tgccagatga
     2101 ggtcgttcgc gcgcaggctg gcgaaggtga gggcgagttc gcgaccgggc agcaagccgc
     2161 cctcggcgaa atcgaactcg cgtttcgcca cgtaagcgtc gtccacgaaa accgacagct
     2221 cgccggtgtc gtgaaagtcg agcatggtcg cgagcagcgt caggctggcg gcgcgatcct
     2281 cccgccgggc gcgcaacacc gcgagcgccg agccgagcag ggtgcccccg acgcagaaac
     2341 cgagcgcatt gacctggccg gcgccgcaaa tttcacccgc gaccgccagc gccttgatca
     2401 cgccctgctc gagataatcg tcccaggttg cgcgtcccat cgcgggcggc atgttgcgcc
     2461 acgacaccat gaacacggtg tgaccgcgct cgacggcgta gcgcacgaag gagttctccg
     2521 gctggagatc gagtatgtag tacttgttga tgcacggcgg caccatgagg agcggccgtt
     2581 cgaacacggt ggccgtcgcg gggcggtact ggatcagctg catgaagtcg ttctggtaga
     2641 tcaccgcgcc cggcgtgatg gcgaggttgc gcccgacctc gaaggcggtc tcatcggtca
     2701 tcgagatcat gccgcggtcg aggtcggcgg cgaggttgcg caggcccttc gccaggcttt
     2761 ccccctcggt ttcggcggcc agccgtatcg cctcgggatt gctccacggg aaattggcgg
     2821 gcgacatggc atcgacgaac tggcgcgcgt agaaatcgag cttgcgcttg gtccggggat
     2881 cggtcctggc ggcatccacg acctcgcgca accactgcgc cgtcagcagg taggactgcg
     2941 cgaggtaatc gaagtaaggc tccttgcgcc attccggggc gcgaaaccgg cggtcggagc
     3001 tgtcgggctc gatgaggggc tgcgccggct tgccgggctg cggttgcgcg taggccgccc
     3061 acaactcgag ccgcttgcgg tagtagcggt tctggatctc gagccagcgc ccggcatcct
     3121 gggtgacgct ctcggtcagc gagcgcagta cggccgcaat atcgaagccg gcgtcgaggt
     3181 cgagcggctt ggcgacgccg ttcacgagct gtcggttgag catctcgatg cagctgcgca
     3241 tcaagtcagc gttgtcggcg gggcgttcca tggcgcggga ctgacagtgt aatcccggtg
     3301 aattgcagaa tcgagaagga gaaggaatat caccggcgcc caatgcttcg caccatggcg
     3361 gtgcccgtac ttgcggctgc gcgccggtga ggcggcctca ggcgtgagta tgcgcgatat
     3421 cgcccaccag ccagatggtc agcacgccga gcgcgatcag caccacctgc tgcaacgtcg
     3481 cctggagctc ggggcggcgg tgcaggccgg gtatcaggtc ggccaccgag acgtagagca
     3541 tgctggcagc ggcgagtccg agcagcgtcg gaatccattg ctgcagcgat tgcagcgcgt
     3601 aataggccag cacgccgccc gccaccatgg ccgcgctgga aaggaggttg aagaacatcg
     3661 cccggctctt gctgtagccc gagtgcagca ggatcaggaa atcccccact tcctggggca
     3721 cttcgtgggc gatgatcgcg atcgcggtga cgatgcccag ttcgtgattg gcgagaaacg
     3781 cggcggcgat cagaatcccg tccacgaaat tgtggaaggt gtcgccgatc atgatcatca
     3841 tgccgctgcg gccgtgatcg tgattgtgat tcggcgcggc ctgcgggtcg tgcgcctcgc
     3901 attgctcgac gtggcaatgc cgccacagca cgagcttttc cagcacgaag aacaacagga
     3961 tgcccgcgag tatggtggcg gcgaggttct cgaggctgtc ggtcgtgccg aaagcatggg
     4021 gaagaatctc caggaacacc gcgccgagca gcgcgccgac cgcgtaactg atcaggatcg
     4081 gcacccagga cgcgcgcgcg ttgaatgcca cggcggcagc cgcaacgacc gagagcacgc
     4141 cgccagccac ggtcgccgcc agtatccacg cgagggtcat cggaattccg gaatctgctc
     4201 aaacgaggcg cggattatac gtgatttggc ggcgccgaat cggacaagcg tgaatcgtga
     4261 ttggtgaata gtgaatggag agtcgagaag cggttttatc cattcaccac tcgccattca
     4321 ccactcacgt ctcatcaatc cgtgcgccag atgaccgccg ccatgcggcc cgtcgcgcgg
     4381 tcgcggcggt aggaatagaa gcgctgcggg tcggagtagg tgcacagttc cccgccatgg
     4441 atgctccgga tgccggcgcc ggcgagccgc tggcgcgcca gcaggaagag atcggccagc
     4501 cattttcccg gcgtgtgcgg tgcaaacgca gcctccgcgc gcgcgtcgcg cgccaggaat
     4561 gcttcgcgca cgtccgcgcc cacctcgaac gcgtgacggc cgatgccggg gccgagatag
     4621 gcgatcaact cttccgcggc taccgtcatc cgttcaaccg cattttcgat caccccgccc
     4681 gccagcccgc gccagccggc gtgcgcgatg cccacggtgg cgcccgcgcg gtcggcaagc
     4741 aggaccggga tgcaatcggc caccagcacc gcgcaaaccg ttcgcgggcg gcgcgcgacg
     4801 gcggcgtcgg cctcgggcac gccgttcaga tcgtccgcct cgacgacgag gctgccgtgc
     4861 acctggcgca gccatttcgg ctcctgcggc agcgccgccc gcagccgttg ccggttggcg
     4921 gcgattgcct ggggcgagtc gtccgtccgc agtcccagat tgaagctcgc gtatgcgccg
     4981 ccgctgatcc cgcctgcccg cgtggtgatg aaggccttga cgttggccgg cgccggccag
     5041 tcgggaacga tccagtccgg atgcagcgat ttcatttgat acggcgtgat tcgtgattcg
     5101 tgattcgaga ttcgcgtcca ggcggaattt caccgcagcg cagagcggcg atcagttcgt
     5161 gcatgtcctg cggcgggtct gcctgccaga tcatcggttt tcgggtgcgt gggtgcagca
     5221 gcccgagtcg ctccgcgtgc agcgcctgcc gcggaaatgg aatggcgccg ccgggcttgc
     5281 cgtacgccgg atcgccgatc agcgggtgcc tgagcgaggc gaggtgcacc cggatctggt
     5341 gcgtgcgccc cgtttccagc cggcagcgca gcagggtcgc ccgctcgtag cgttcgagca
     5401 cctgatagtg cgtcaccgcc tgccgtcccc gcgccgttac tgccatgcgg gtgcgtttta
     5461 gcggctggcg gccgatcggg gcatcgatcc tgccgtcgcg cgcgacacgg ccctgcacga
     5521 gggcgagata ctcgcgcttg acacttcgcg ccttgagctg gcgcacgaga tcggtctggg
     5581 cggcgagcgt tttcgccacg accagcaaac cgctggtgtc cttgtcgagc cgatgcacga
     5641 tgcccgcgcg cggaatgccg gcaagcgcgg gggcgcgtcc gaggagggcg ttcaagagcg
     5701 tgccctgcca gttgccgctg ccgggatgca cgacgagccc ggccggcttg ttgacgacaa
     5761 gcagcgcgtc gtcctcgtac acgatatcga gcgccatgtg ctccgcgcgg tgcacggtcg
     5821 tcgcgggatc gggggtggga atgacccgga tccgctcgcc ttcccgcact ttttgccgcg
     5881 gcggcgcggc gcggccctcg agcgtgaccc ggcccgcctt gatccactgc gcgagccggc
     5941 tacgcgagta ttccggaacc agattcgcga gcgcctgatc cagccgaagg ccggcgcatc
     6001 cggcgggcac gacgagctcg accgggctca tgccggaatc ggttgagtta taattttccg
     6061 aaggcttcat cgtgctcatc gttcgccata gtttagcgct gttgctcggc gtgctcatcg
     6121 gggcgtgcgg cctgctgccc gaagagcagg atgaaaccaa aggctggtcg gcgcagaaga
     6181 tctatgccga ggcgaaggat aacctcgaca aggccaacta cgagcaggcg atcaagtact
     6241 accagaagct cgagtcgcgc tatccgtacg ggcgcttcgc ccagcaggcc cagatcgaga
     6301 ttgcctacgc ctattacaag gacagcgagc cggtgcaggc cgtggttgct gcggaccgct
     6361 tcatcaagct ccatcccaac catcccaacg tggactacgt ctactacctg aaaggcctcg
     6421 cgaatttcaa cgacgacctg ggatggacgg gcttcatcag cgggcaggat ttgagcgagc
     6481 gcgaccccaa agcggcgcgc gaggcgttcg acgcattcaa ggagctggcg acccgctacc
     6541 ccgggagccg ttatacgccg gatgccattg cccgcatgaa ctacctgctg aacgcgctgg
     6601 cctcgcacga agtccacgtc gcgcgttatt acatgaagcg gggtgcctat gtcgccgcgg
     6661 ccaaccgcgc ccagtacgcg ctgaagacct atccccaggc gccggccaac gaggaaggcc
     6721 tcgtgatcct ggtcagggcc tacgatgcgc tcggcatgga cgacctgcgc aacgacgccg
     6781 agcgtgtcct gaagaagaat ttcccggaca gcgtttacct gaaaggcggc ccccagggca
     6841 ccgctccctg gtggcagttc tggaactggt aatcgccgcg cagaggcgat taccagttac
     6901 aaggttcagg ttgcaaggta caagataagc gccgtgcctc gagtcttgta acttgtaact
     6961 tgaaaccgag gcgttatccc agcgcgaggc agatgatgcc ggcgaccatc gcggccgccg
     7021 cggccagccg ttgcgcgaca ttgccttcgg ccagcaggtg ggttcccatc agcgcggcga
     7081 ccaggatact cacctcccgc gccggcgcga catagctcac cggcgtgaac accatggcgg
     7141 tgagcaccag gatgtaggaa agcgggcaga gcagcgcgac cgcgatgatc tcctttttcg
     7201 cccgccgcca ggtgggaaca atctcgtgcc cgcgaccgag catcatcggg gtcatcagca
     7261 ccacgcggcc gaaattggtc gcccagtcct gcaccagcgg cgggatgagc caggcggcga
     7321 cggcgatctt gtccaccagc gtgtagctgg cgatcatgca gccagtcagc agcgcgaaac
     7381 cgaccgcgtg aagatttccc gactgcctga gcttgcgcgg gtctcccgtc aatgcgaccg
     7441 cgccgccgcc gatgagcaac gcgccggcca cggcgagcgc gctcgggtcc tctcccagca
     7501 gcagcaccgc gcacagcacc gtgatcagcg gcccggtgcc gcgcgcgata ggataaacca
     7561 cggagagatc gccgctgcgg taaccgcggt cgagcagcag gtaataagcg gtatgcagca
     7621 cggcgcttgc cagcatcatc ccgtaatgct cccagccgaa ttccggcttc tgccaccaga
     7681 tgatgaaggc cgcgagcggc aggtagatca gcgcggagag gctggcaaac agccacacga
     7741 aaacggcgcc gccgccggag cgcttgagga agtagttcca gctggcgtgt atgacggctg
     7801 cggcaagaat cagggcgaga gcgaacgcgg tcatgatgtc ctcgaggtca aaaaagcatc
     7861 gttgatagcg aagaggcgta gtgaaacacc tcaacctatc ccctctggct ttttagtcct
     7921 cgggtgcatg ccgcgcgggc tttgtgtagc gcttggtcca ggcctggctc gacgacatgc
     7981 cagcggaacc ctagctaccc gtcggttgaa attcgattac agccgatcgc ccgcatggcg
     8041 tcaagcagcg ggggtgctca ggacgtgagc tcgggccggt ccttgtaatg cgcgagtgct
     8101 tccgggttgg cgagcgcttc cacgttccgg acgggccgcc cgtgcaccac gttgcgcacg
     8161 gccagctcca cgatcttgcc gctcttggtg cgcggaatgt cgggcacctg cacgatgcgc
     8221 gccggcacgt gacgcggcgt ggtgttgtcg cggatcttgc gcttgatccg ctcgatcagt
     8281 gcgtcgtcga gctgcaggcc ctcgcgcagc cgcacgaaca gcaccatgcg cacgtcggcg
     8341 gggcggtccg ggggccagtc ctggccgatg acgaggcttt cgatcacctc gtcgaggctc
     8401 tcgacctggc ggtagatctc ggcggtgccg atgcgcacgc cccctgggtt cagcacggcg
     8461 tccgagcgcc cgtagatgac gaacccgccg cgcgccgtct gctcgatgta gtcgccgtgg
     8521 caccagacgc ccgggaattt ctcgaaatag gccgcgcggt acttggcccc atccgggtcg
     8581 ttccagaaac cgaccggcat cgagggaaag ggcacagtgc agatcagttc gcctttcgcg
     8641 ccgcgcaccg ggcggccttg ctcgtcgtac acctcgactt tcatgccgag cccggcacac
     8701 tgaatttcgc cgcgccatac cggcgcaatg ggatttccga gcacgaagca gcctatcaag
     8761 tcggtgccgc ccgaaatgga ggcgaggtga agatcgctct tcacttcccg gtagacgtag
     8821 tcgaagccct ccgggaccag cggcgagccg gtggacagca gggttttcac ggatgtcagc
     8881 cggtgcgtca gccgcggctt caggcccgcc ttggccgcgc tgtcgatgaa cttggcggag
     8941 gtgccgagca cggtgatgcg cgcggcgtcc gcgtaatcga acaggatgcg ccctccggac
     9001 atgaacggcg agccgtcgta gagggcgatg accgcgcgcg aggcgagcgc cgaggcgagc
     9061 cagttccaca tcatccagcc gcaggtcgtg aagtagaaca ggcggtcccc gggcttgaga
     9121 tcgacgtgca gctggtgttc cttcaggtgc tggatgaggg tgccgccggc gccgtgcacg
     9181 atgcacttgg gcacgccggt ggtgccggag gaatacatga tgaagagcgg gtggtcgaac
     9241 gggaagcgct cgaaccggat atccccggcg cgccagggcg ccatcaggtc atggacgttg
     9301 accgcgcggg gcaggcccgt tagcggcggg cgcggccggg tataggggac cacgatgagt
     9361 ttctcgatcg aaggtatcgc accggcgatc tccttcaggc gcggcagcag gtcgatcgtc
     9421 ttgccgttgt agaaatagcc gtcggccgca aacaatatct tgggggcgat ctggccgaaa
     9481 cggtcgagca cgccctgcac cccgaaatcg ggggagcacg aggaccagat cgcgccaaga
     9541 ctggttgccg ccagcattgc gataaccgtg ccgggcacgt tgggcatgta cgcggcaacg
     9601 cggtcgccgg gcttcacgcc ggcggcctgc aatgcctgcg agaggcggga cacctcggag
     9661 tggagctcgg cgcaggtgac cgtcgtcttc agccggtctt cgccccagaa cacgatggcg
     9721 accgagcggt cgcggcgtcg caggaggttt tccgcaaagt tgagccgcgc gtccggaaac
     9781 cagcgcgctc ccggcatgcg acggccgtcc tcgagcgccg gtccatcgcg ttcatccccg
     9841 atgacattgc agaaagacca gaccgattgc cagaactggg ccggctccgt gacggaccag
     9901 cggtgcaggc ccggaaaatc gttcgccgcg atgccccagc gctcgcgcgc ctggcgcgcg
     9961 aacgcggtca ggttggcgtt ccggacgcgc tcgggagaag gctgccagag cggcttgtca
    10021 gcgggcgggt tcaaggtcgg tcaatagaca ggattaacag aacaactcaa aacccttcaa
    10081 ccccgttaat agacaggatt aacagagctc ctcaagaacc cgtcaacccc attaatagac
    10141 aggatttaca agattaacag gattcaaaca acaatcattt ttcgttttgt ttttgtgaat
    10201 gccttaatcc tgtaaatcct gttaatcctg tccatttgtg ttcttgacgg gttttgttaa
    10261 tcctgtccat ttgtgttctt gacgggtttt cactccgggc ggagcagggg aaacaggatc
    10321 acgtcgcgga tcgaggggct gtcggtcaac agcatcacca gccggtcgat gccgataccg
    10381 gcgccggagg cgggcggcat gccgtattcg agcgcgcgga tgtagtcggc atcgaagtgc
    10441 atggcctcgg cgtcccccgc ctctttctgc ctggcctgct gcgtgaagcg ctcggcctgg
    10501 tcctccgggt cgttcagctc ggagaagccg ttcgcgatct cgcgccccgc gatgtagagc
    10561 tcgaagcgct cggtgatttc cgggttcctg tcgctgcggc gggcgagggg agaaacctcg
    10621 gtgggatagt ccacgatgaa ggtcggctgg acgagctggt cctcgaccgt gttctcgaac
    10681 agcgtcaact gcagcccgcc gatgccgtcg ccttcgcgat aggcgatatt gcgttgcttc
    10741 agcgcggcga tcagagcggc gcggtcgttg aggatagcgt cggtgatccc gggatggtat
    10801 ttccggatgg cctgcctgat ggtgaggcgc tcgtacgggc gcgcgaagtc gatctcgctg
    10861 ccctggtagg tgaccctggt tgcgcccagc gccgcctgcg cggcatgggc gagcatcctc
    10921 gggatcacgg ccatcatgta ctcgtagtcg gtatacgccg cgtagaactc gagcatggtg
    10981 aattcggggt tgtgccgggt cgagatgccc tcgttgcgga aattgcggtt gatctcgaac
    11041 acccgttcgc agccgcccac caccagccgc ttgaggtaca gctccggcgc gatgcggagg
    11101 tagaggtcca tgtcgagcgc gttgtggtgc gtggcgaagg gccgcgccgc ggccccgccg
    11161 ggtatcggct gcatcatcgg cgt
//
LOCUS       DBNGSG010000344         3653 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2488286, whole genome shotgun sequence.
ACCESSION   DBNGSG010000344 DBNGSG010000000
VERSION     DBNGSG010000344.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2488286"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..1353
                     /locus_tag="AC158Q_08975"
     CDS             256..1353
                     /locus_tag="AC158Q_08975"
                     /inference="COORDINATES: protein motif:HMM:NF024771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR3339948.1"
                     /translation="MKIKSIKAGVHRLAVSVPLLKEPINRRTVFCEVETDDGHVGHGI
                     TGGAYLPLAIVTAIEKEFFPLLKDMDPRDTEAIHEKVWWKLNQRSMTGVVSSALSAID
                     IACWDIRGKHAGRTVAQLLGGARDWAPTYCTFGFTEYEDREQLVEAAKLQLKNGHRRL
                     KMVVAVHKGGWQEDARRVRAVRDAVGPDVELMMDANYKFNPVDARQLCRAIEDCRLTW
                     FEEPVYQNDTRAMADLRAHTGIPIAAGQNEGHRWRLRELVLHQAVDILQPNVCYCGGF
                     TETQKAGHLAQAFNLPIANGGGWPLHNLHIMAGLMNGWRVEFHLDMQAVGELLFVNPP
                     HPENNIVKVPGKPGLGLEPNRDVLKDSLIKA"
     gene            1428..1823
                     /locus_tag="AC158Q_08980"
     CDS             1428..1823
                     /locus_tag="AC158Q_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005431054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HIR3339949.1"
                     /translation="MSEEISLWVLFSSSFLAATLLPGGSEAVLFGALKLHPEQVLPAL
                     GVATLGNTLGGMSSYLIGRILPQKKDLPGLPALRRYGTPALLLSWVPVLGDPLCVAAG
                     WLRLNPWLSMLFIALGKFARYAAIAAFAT"
     gene            1911..3017
                     /locus_tag="AC158Q_08985"
     CDS             1911..3017
                     /locus_tag="AC158Q_08985"
                     /inference="COORDINATES: protein motif:HMM:NF022447.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HIR3339950.1"
                     /translation="MSAVNLKLSTRNLKLESFFSPDYAAARDRFRAAAHAAGASLDAL
                     GIEARGPRQERLTIDIARLGAPDARRVLLHTSGLHGVEAYAGAAVQLAALESPPVPPA
                     GCALVLAHVLNPYGMAWSRRANENNVDLNRNFLRRDEPWEGAPDLYLRLDPLLNPAAP
                     PGPDGFRLRLALLALRHGVRAPRQAIAEGQYEFPRGLFYGGKALEPGPRLYLDWLHRN
                     LAHADYLLALDLHTGLGRRGEETLILEPAVNATPAAGLGQALGKPLSDPATGRTAYRI
                     RGGMGGVLPQALPRARIDFVLQEIGTCSALAVLHALREENRLHHHGKSSLTHPAKQAL
                     CEALNPASVDWRRHAVGHGLSLLHAAAAWTFEKQ"
     gene            3116..3532
                     /locus_tag="AC158Q_08990"
     CDS             3116..3532
                     /locus_tag="AC158Q_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIR3339951.1"
                     /translation="MACELCETDGGELIWRDGRCRVVYVAEPGYPGYCRAIWNTHVAE
                     MTDLTESERAGFMRVVFTVEAELRERLRPDKINLASLGNVVPHLHWHVIPRFRDDPHF
                     PNPIWSGQRQRPDASPRAHIDITRTLAERLSRLKSP"
BASE COUNT          620 a         1224 c         1171 g          638 t
ORIGIN      
        1 cgatacctgc gggccgttct tgccgagctt gcgcttgtcc atttcgttct cctcgcttac
       61 acgtggttag tcttctgatc cctgctcccc ggccgcgagc ggattatctt tgatgcgcgc
      121 cgcaggcgtc aatttagcgc agcgcgcgcg ggtgtggtcg acgccgcgcg aagcgccgcc
      181 ggtgtaggtc gacgccgcgc gaagcgccgc cggtgtacac ttcgcgcgac ctgcgcatcc
      241 ccctggagaa tcccgatgaa gatcaagtcc atcaaggccg gtgtccaccg cctcgccgtc
      301 agcgtgccgc tgctcaaaga gcccatcaac cgcaggacgg tgttctgcga agtcgagacc
      361 gacgacggtc atgtcgggca cggcatcacc ggcggcgctt acctgccgct cgctatcgtg
      421 accgcgatcg agaaggagtt tttcccgctg ctgaaggaca tggatccgcg cgacaccgaa
      481 gcgatccacg agaaggtctg gtggaagctc aaccagcgct ctatgaccgg cgtggtgtcg
      541 agcgctctct ccgccatcga catcgcgtgc tgggacatcc gcggcaagca cgccggccgc
      601 accgtggcgc agctgctcgg cggcgcgcgc gactgggcgc cgacttactg cacgttcggc
      661 ttcaccgagt acgaggaccg cgagcagctg gtcgaggcgg cgaagctcca gctcaagaac
      721 ggccatcgcc gtctcaagat ggtcgtggcg gtgcacaagg gcggctggca ggaggacgcg
      781 cggcgcgtgc gcgcggtgcg cgacgcagtg ggcccggacg tcgagctcat gatggacgcc
      841 aactacaagt tcaacccggt ggatgcgcgc cagctttgcc gcgcaatcga ggactgcagg
      901 ctcacctggt tcgaggagcc ggtctaccag aacgacaccc gcgccatggc cgacctgcgg
      961 gcccacaccg gcatcccgat cgcggcgggc cagaacgagg gacaccgctg gcgactgcgc
     1021 gagctcgtgc tgcaccaggc ggtggacatc ctgcagccca acgtttgcta ctgcggcggc
     1081 ttcaccgaga cgcagaaggc ggggcacctg gcgcaggcgt tcaatcttcc gatcgccaac
     1141 ggcggcggct ggccgctgca caacctgcac atcatggcgg ggctcatgaa cgggtggcgg
     1201 gtcgaatttc acctcgacat gcaggcggtc ggcgagctgc tcttcgtgaa tccgccccac
     1261 cccgagaaca atatcgtgaa ggtccccggc aagccggggc tcggactgga gcccaaccgc
     1321 gacgtgctca aggactctct gataaaggcg tgattcgtga atcgtggttg atgattggtg
     1381 ttcacacctc acacctcacg cctcacccct ctccccctcg ctcttcgatg agcgaggaaa
     1441 tcagcctgtg ggtgttgttt tcgtcgagct tcctggccgc gacactgctg ccgggcggct
     1501 cggaagccgt gctgttcggc gcgctcaagc tccatcctga acaggtcctg cccgccctcg
     1561 gcgtggctac gctgggcaac acgctgggcg gaatgtcctc ctacctgatc ggtcgcatcc
     1621 tcccgcagaa aaaagacctg cccggcctgc cggctttgcg ccggtacggc acgccggcgc
     1681 tgctcctgtc ctgggttccg gttcttggcg atcccttgtg cgtggcggcg ggctggctgc
     1741 ggctcaaccc gtggctttcc atgctgttca tcgcactcgg caagttcgcg cgctacgccg
     1801 cgatcgccgc attcgcaacg taagagcggc gcgtcaagct ccggacactg ctctggcgac
     1861 agttttaggt ttaaggtttc aagtataaag ttggggcgat agccgcacgg atgtctgccg
     1921 tcaacttgaa actttcaact cgaaacctga aactcgaatc tttcttctcg cccgactacg
     1981 ccgccgcgcg cgaccgtttc cgcgccgcgg cgcatgccgc gggagcttcg cttgatgcgc
     2041 tcggcatcga ggcgcgcggg ccgcggcagg aacgcctgac catcgacatc gcacgcctcg
     2101 gagcgcctga tgcgcgcagg gtcttgctgc acaccagcgg gcttcatggc gtggaggctt
     2161 acgccggcgc cgcggtgcaa ttggccgcgc tcgagagtcc cccggtcccg cccgccggct
     2221 gcgccctggt gctggcgcat gtgctcaatc cctacggcat ggcgtggtcg cggcgcgcga
     2281 atgaaaacaa tgtcgatctc aaccggaact tcctgcggcg cgatgaaccc tgggagggcg
     2341 cgcccgatct ttatctccgc ctcgacccgc tgctcaaccc agccgcccct ccggggccgg
     2401 acggtttccg gctgcggctc gccttgctcg ccttgcgcca cggcgtgcgg gcgccaaggc
     2461 aggcgatcgc ggagggccag tacgagttcc cgcgagggct gttctacggc gggaaggcac
     2521 tggagccggg gccccgcttg tacctcgact ggttgcatcg caacctggcg cacgcggatt
     2581 acctgctcgc gctggacctg cacaccgggc tcggccgccg cggcgaagag acgttgatac
     2641 tggagcctgc cgtcaatgcg acgccggcag ccgggcttgg gcaagcactc ggaaaaccgc
     2701 tcagcgaccc ggcaaccgga cggacggcat accggatacg cggcggcatg ggcggcgtgc
     2761 tgccgcaagc gctgccccgg gcacgcatcg atttcgtgct gcaggaaatc ggcacctgct
     2821 ccgcgctcgc cgtgctgcac gccttgcgcg aggagaaccg cttgcaccat cacggcaaat
     2881 ccagcctcac tcaccctgcc aagcaggcgc tctgcgaagc gcttaacccc gcatccgtgg
     2941 actggcgccg tcacgccgtg ggtcacggcc tgtccctgct gcacgcagcg gcggcctgga
     3001 cgtttgaaaa gcaatgatga atgcagaatg atgaatgcgg aatggagcag cacggacttt
     3061 gcgcgagggt tgtttcccat tcatcattca tcgctcatca ttcggcgttg aaattatggc
     3121 ctgcgagctg tgcgagaccg acggcggcga gttgatctgg cgcgacgggc ggtgccgggt
     3181 ggtttacgtc gccgagcccg gctacccggg atactgtcgc gcgatctgga acacccacgt
     3241 agccgagatg accgacctca ccgaatccga acgcgccggc ttcatgcgcg tcgtgtttac
     3301 ggtggaagcg gagttgcgcg agcggctgcg gcccgacaag atcaatctgg cgagcctggg
     3361 caatgtcgtg ccccatctgc attggcatgt gatcccgcgt ttccgcgacg accctcattt
     3421 ccccaacccg atatggagcg gacagcgcca gcggccggac gcctcgccac gggcgcacat
     3481 cgacatcacg aggacgcttg ccgagcggct gtcccggctc aagagcccct aacataatga
     3541 aacgcgcgct gcgctgtcac gattttggct cagggcgcga agcgagccgc agccaatatg
     3601 tcgatattgg caaggcgagc gacaaagcca tgggccaaaa ctcgtgccag ccc
//
LOCUS       DBNGSG010000345         9399 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_792065, whole genome shotgun sequence.
ACCESSION   DBNGSG010000345 DBNGSG010000000
VERSION     DBNGSG010000345.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9399)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9399)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9399
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_792065"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="AC158Q_08995"
     CDS             <1..347
                     /locus_tag="AC158Q_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339952.1"
                     /translation="RLQAERDRLQKEAMQEYANRPGSWWEPLLFMAAMWSIPLYFILQ
                     IVLLLRWRGGWRIAAAIPAVPMLLVLGHAIFAFFAGSNIFPIVLVFTCLPVLIYLLVL
                     IALRRFRLQPAP"
     gene            complement(354..2510)
                     /gene="pbpC"
                     /locus_tag="AC158Q_09000"
     CDS             complement(354..2510)
                     /gene="pbpC"
                     /locus_tag="AC158Q_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HIR3339953.1"
                     /translation="MKRVLLLGASGALCAAAIAFAISLAIAPYPVPEFESVRGAWRAS
                     DAWLLDRNGEPLSRVRVDRERRRGEWLAARDVSPALIDMVLASEDRRFLEHGGVDWIG
                     MLGAFRQTAAGERRGGSTLTMQLAAFLNPELETGGRRGPVDKWRQMRQALALERAWDK
                     DRILEAWLNLTPFRGEVEGVDAGARALLGKRAGGLDRVEGALLAALVRSPNAPSTRVA
                     ARACVLLGREEGACLAAQGLAAAGLRAGRARFEIEGDAPHLARKLLTRPGERRASTLE
                     ARVQRFAAETLQRHLRELEGRNVEDGALVVLDNATGEVLAWVGSSGELSRAREVDGVL
                     ARRQAGSTLKPFLYALAIERRLLTAASLLDDSPLAVTTPAGLYVPQNYDHSFKGRVSL
                     RLALASSLNVPAVRTLALMGYEPFYRQLKALGLETLERDADHYGYSLALGGGEVSLLG
                     LANAYRALANGGRAAPVRMLPAAGDGAVPVQVVDPAAAFIVGDILADAGARSLTFDLA
                     GPLAARYPASVKTGTSKDMRDNWAVGYTARYTVGVWVGNFSGEAMHDVSGVTGAAPVW
                     REVMDFLHEGGVPAHPAPPAGVVRQRISYADGMEPAREEWFLAGTEAAKLVAVDAVAG
                     RARISSPPDGAIFAIDPDIPRERQRIALAAHGARPGTWLRLDDGRRVRADAPYLWLPQ
                     PGRRQVALLDTKGKEIDRVRFEVRGLRPAAKRIRPD"
     gene            complement(2507..8359)
                     /locus_tag="AC158Q_09005"
     CDS             complement(2507..8359)
                     /locus_tag="AC158Q_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HIR3339954.1"
                     /translation="MGLRAAVLNQAIGAIAFAAAAGAAWAGPRVDVFTPQGAAKGVRQ
                     VAVRFSEPMIAFGDPRLPDPFTVRCDGDPARLKGRGRWADQKNWAYDFEAELPAGQAC
                     TFMLKADFKSPGGQAVEGRRDFRFHTGGPAVMASLPYEGEEHVDEEQAFLLALDAPLD
                     AASLSDAWCEARGVNERIPLRLLSEGDTRELLAANRDRAYRLYNAYFKGRHRFPAASF
                     KIEDKRLRELPVIGVRCARRLPAGAEIGLVLGPGVQSKTGLPRETPQRLAFRVRPAFF
                     VKLTCRRVNREAACLPVAPITLDFNAPVPREAAARIRLKGGGRTFEPTLEPNVKTVES
                     VEFPGPFPEKTRYTVGVPGDFRDDAGREPENKASFPLATATDENPPLVKFPGRFGILE
                     LNADPVLPVTVRNVESPLQGRRAELPSGAERPIAGRSARIGDDETRIIERYRAFREND
                     RDHPARAALKRYPNEGELPVILDSDKAVAFQLPRPGGEKAFEVVGIPLPRPGFYVVEL
                     ASPRLGRALHGEDRPYYAATSVLVTNLAVHLKHGRESSLVWVTSLDRGKPVAGARVTV
                     RDCTGRLWFEGRTDAQGIAHAGDRLPAQAEVPACKDYRRELIAFARLGEDLSFTFSSW
                     NEGIRPWNFNLPANLYRLKPLAIHTVFDRTLLRAGETVSMKHVARVPAGAGFRVPQGT
                     EMPAAAEIQHIGSGQKYPLGARFDAGGIAESTWKIPAEAKLGFYRLYWPGRGEIQGNA
                     QFRVEAFRVPLMKAVLAAPREPLVKPVAAKLDAAVSYLSGGPAGGLPVKVRYRVEPRG
                     VSFADYPDFHFGGAAVKEGMQTGSVEDLWATFDPDEDDAGPAPAERAGPAAARTLVLD
                     PAGTASVVIDKLPPIDRPGSLLVELEYSDPNGELLAASARVALHPAGVYLGIKPEGWA
                     ANRKGVRAQVVALDPLGKPLAGRAVSVEAYERKTYSNRRRLLGGFYAYDSTTETRRAG
                     AGCSGTTDARGLMFCSVKPGTSGELILVARAKDDGGRETFSTSSVWVLGDGEWWFEPS
                     NNDRIDLVPEKKRYEPGETARFQVRMPFREATVLVTVEREGVLAQKVVALDGKSPVIE
                     VPLTGSYGPNVFVSALAVRGRVDPEVPGPYAWLKRTAYRVGHWFGLVDAVPVERDTRP
                     TALVDLSRPAYKLGMAEIKVGRRDYVLNVKVTPERETFRVRETARIAVEVTDSDGKPA
                     AGGEIALAAVDEGLLELMDNASWNILDALLGNRPVEVQTATAQGQVIGKRHFGRKAAP
                     PGGGGGRAGARELFDTLLAWKGRVALDERGRAVVAVPLNDALTGFRIVAVAVAGEARF
                     GHGMATVRTTQDLMLFSGLPPVVREQDEFTAMFTLRNTTAQPVAAALAWELRDRPAGD
                     AKGRTLAKGSQAVPLAAAEAKLIPVPAQAPAGVERLYWEITATGKGGATDRLRTGQKV
                     VEVHPVRVYQATLAQLDKPLQFPVERPAGAIPGRGGIRVDVMGTLAGEMSAVREYFLR
                     YSYTCLEQRASKAIGLADDDLWKSVTGTVGNYLDRDGLARYFPIDRLQGSDALTAYLV
                     QVADAAGREWPEEPLKRMLSGLEAFATGRITRGSALPTADLTVRKLAAIEALARHERA
                     RPAMLESITIDPALWPTSALIDWIGILKRVEGIRHHDQHLREALSQLRARLNFQGTVM
                     TFSTERTDALWWLMVSADVNANRALLAVLDEQGWREDVGRLVRGSLSRQKRGRWGTTV
                     ANAWGVIAIARFAEAFEKTPAGGTASVTLGSRSAPVKVGAAKQTRDFEWPAGRETLDL
                     AHAGSGAPWAIVQSRAALPLKAPLSTGYAIRRTVAPVEQKEKSGYTRGDVYRVTLEID
                     AQTDMTWVVIDDPIPAGAAILGSGLGRDAGSLAQGEKQAGWAWPAFIERTHEAYRAYY
                     EFVPRGRFRIEYTVRLNNPGRFELPATRVEAMYSPELFGETPNAAVAVKP"
     gene            complement(8384..>9399)
                     /locus_tag="AC158Q_09010"
     CDS             complement(8384..>9399)
                     /locus_tag="AC158Q_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339955.1"
                     /translation="DETRVTHPIALASSAIDVALKLDHRRKGLLWYSTYAVDFSAVYA
                     VVNTTGAAREIEIALPFPAKNAVFDDLQLEVAGKEWIAPPRPSHDRIVGSVRLAPGER
                     VNVRAGYRSQGLGRWSYSFGSGVSEVRDFRLTMRTDFHAIDFPDDGISPGRKERAGGG
                     WALTWEFRRLVTGVNIAMLMPEKLQPGPLASQISGFAPISLFFFIVVMLTIGVVKRVE
                     LHPMHFFFLSAAFFSFHLLFAYLADLVSIHAAFAIAAATSLALVVSYLRVMFGNRFAF
                     FAAGAAQFVYLVLFSYAFFFKGLTGLAITLGAIVTLFVLMRATAKVEWSEIFGGRAAR
                     TPA"
BASE COUNT         1337 a         3495 c         3234 g         1333 t
ORIGIN      
        1 tccggctcca ggccgagcgc gatcgcctcc agaaggaagc catgcaggag tacgccaacc
       61 gcccgggatc gtggtgggag ccgctcctgt tcatggccgc catgtggagc atcccgctct
      121 acttcattct gcagatcgtg ctgctcctgc gctggcgcgg cggctggcgc atcgcggcgg
      181 cgatcccggc cgtgcccatg ctgctcgtcc tcggccatgc catcttcgcc tttttcgccg
      241 gcagcaacat tttcccgatc gtgctggtct tcacctgcct gcccgtgctg atctacctgc
      301 tggtgctgat cgcgctgcgg cgcttcaggc ttcagccggc gccgtagccg gactcagtcc
      361 ggccggattc gtttcgcagc gggccgcagg ccgcgcacct cgaagcgcac gcggtcgatc
      421 tcttttccct tcgtatccag cagggcaacc tggcgccggc ccggctgcgg cagccagaga
      481 tagggcgcgt cggcgcgcac gcggcggccg tcgtcgagcc gcagccacgt gccggggcgc
      541 gcgccgtggg cggcgagcgc gatgcgctgg cgctcgcgcg ggatgtcggg atcgatggcg
      601 aagatggcgc cgtcgggagg cgagctgatg cgcgcgcgcc ccgccacggc atccaccgcg
      661 acgagcttcg ccgcctcggt gccggcgaga aaccattcct cgcgcgcggg ctccatgccg
      721 tccgcgtaac tgatgcgctg gcgcacgacc cccgccggcg gcgcaggatg cgcgggcacg
      781 ccgccttcgt gcaggaaatc catcacctcg cgccacaccg gcgcggcgcc ggtcacgccc
      841 gagacgtcgt gcatcgcctc gccggagaag tttccgaccc acacgccgac ggtgtaacgg
      901 gccgtgtagc cgaccgccca gttgtcgcgc atgtccttgc tggtcccggt cttgacgctc
      961 gccgggtagc gcgcggcaag cgggcccgcg agatcgaacg tgagcgaccg cgcgcccgcg
     1021 tcggccagta tgtcgccgac gatgaacgcc gcggccggat cgacgacctg caccgggacg
     1081 gcgccatcgc ccgcggctgg cagcatccgc accggggcgg cgcgcccgcc gttcgcgagc
     1141 gcgcggtagg cgttggcgag cccgagcagc gacacctcgc cgccccccag cgcgagcgag
     1201 tagccgtagt gatcggcgtc gcgctcgagc gtctccaggc cgagcgcctt caactgccgg
     1261 tagaagggct cgtagcccat cagcgccagc gtgcgcacgg ccggcacgtt gagcgaggac
     1321 gccagcgcga gccgcagcga cacgcgtccc ttgaagctgt gatcgtagtt ctgcggcacg
     1381 tagaggccgg cgggcgtggt caccgcgagc ggcgagtcgt cgagcaggga ggccgcggtc
     1441 aacagccggc gctcgatcgc gagcgcatag aggaacggct tcagcgtcga gcccgcctgg
     1501 cgccgcgcca gcacgccgtc cacttcgcgg gcgcgcgaga gctcgccgct cgaaccgacc
     1561 caggcgagca cttcacccgt cgcgttgtcg agcacgacca gcgcgccgtc ctcgacattg
     1621 cggccttcga gctcgcgcag gtggcgctgc agcgtctccg cggcgaagcg ctgcacccgc
     1681 gcttccagcg tgctggcgcg gcgttcgccc gggcgcgtga ggagcttcct cgcgagatgc
     1741 ggcgcatcgc cctcgatttc gaaacgcgcc cggcccgcgc gcagccccgc cgcggccagc
     1801 ccctgcgccg ccaggcacgc gccctcctca cgcccgagga ggacgcaggc gcgggcggcc
     1861 acgcgcgtgg acggcgcatt gggcgagcga acgagcgcgg cgagcagcgc gccctcgacg
     1921 cggtcgagcc ccccggcgcg cttgccgagc aacgcgcgcg cccccgcgtc cacgccctcg
     1981 acttctccgc ggaacggcgt gaggttgagc caggcttcga ggatccggtc cttgtcccag
     2041 gcgcgctcca gcgcgagcgc ctggcgcatc tggcgccact tgtcgacggg gccgcgccgc
     2101 ccgccggttt ccagctccgg gttgagaaac gccgcgagct gcatggtcaa cgtgctgccg
     2161 ccccgccgct cgcccgcggc ggtctgccgg aacgcgccca gcatgccaat ccagtcgacg
     2221 ccgccgtgct cgaggaagcg ccggtcctcg gacgcgagca ccatgtcgat gagcgcaggc
     2281 gaaacgtccc gcgccgccag ccattcgccg cggcggcgct cgcggtccac gcgcacccgc
     2341 gacagcggct cgccgttgcg gtcgagcagc cacgcatcgg acgcgcgcca cgcgccgcgc
     2401 acggactcga attccggcac cggatagggc gcgatcgcca gcgagatcgc gaacgcaatc
     2461 gcggcagcac acagggcgcc cgacgcgccg agcaggagta cgcgcttcat ggcttcaccg
     2521 cgaccgccgc gttcggtgtc tcgccgaaca gctcgggcga gtacatcgcc tccacccgcg
     2581 tcgccggcag ctcgaagcgg ccggggttgt tcaggcgcac ggtgtactcg atcctgaagc
     2641 ggccccgggg cacgaactcg tagtaggcgc ggtaggcttc gtgcgtgcgc tcgatgaacg
     2701 ccggccacgc ccagcccgcc tgcttctcgc cctgcgcgag cgagcccgcg tcgcgcccga
     2761 ggcccgagcc caggatcgcg gcgccggccg gaatcgggtc atcgatcacc acccaggtca
     2821 tgtcggtctg cgcgtcgatc tcgagcgtga cgcggtagac gtcgccgcgc gtgtagcccg
     2881 atttctcctt ctgctcgacc ggcgcgaccg tgcgccggat ggcgtagcca gtcgagagcg
     2941 gcgctttcag cggcagcgcc gcgcgcgatt gcacgatggc ccacggggcg ccgctgccgg
     3001 cgtgcgccaa gtccagcgtt tcgcgaccgg cgggccactc gaagtcccgc gtctgcttcg
     3061 ccgcgccgac cttgaccggg gccgaccggc tgccgagcgt caccgacgca gtgccgcccg
     3121 ccggggtctt ctcgaaagcc tcggcgaagc gcgcgatcgc gatcacgccc caggcgttgg
     3181 cgaccgtcgt gccccagcgc ccgcgcttct ggcgcgacag cgacccgcgc acgaggcggc
     3241 cgacgtcctc gcgccagccc tgctcgtcga gcacggcaag cagcgcacgg ttcgcgttga
     3301 cgtcggcgga gaccatcagc caccacagcg cgtcggtgcg ctcggtggag aaggtcatca
     3361 ccgtgccctg gaagttgagc cgcgcgcgca gctgcgacaa cgcctccctc aggtgctggt
     3421 cgtgatggcg gatgccctcg acgcgcttca atatcccgat ccagtcgatc agcgccgagg
     3481 tcggccacag cgcggggtcg atggtgatgg attccagcat cgcgggccgc gcgcgctcgt
     3541 ggcgcgcgag cgcctcgatg gcggcgagct tgcgcacggt caggtcggcc gtgggcagcg
     3601 cgctgccgcg cgtgatgcgg ccggtggcga acgcttccag ccccgagagc atgcgcttca
     3661 gcggctcctc cggccattcg cgcccggcgg cgtcagcgac ctgcaccagg tacgccgtga
     3721 gcgcgtcgct gccctgcaac cggtcgatcg ggaaatagcg cgcgagcccg tcgcggtcca
     3781 ggtaattgcc cacggtcccc gtcacggact tccacaggtc gtcgtcggca agcccgatcg
     3841 ctttcgaggc gcgctgctcg aggcaggtat aggaatacct caggaaatat tcccgcaccg
     3901 cggacatctc gccggcgagc gtgcccatca cgtcgacgcg gatgccgccg cggccgggga
     3961 tggcgccggc cggccgctcg accggaaact gcagcggctt gtcgagctgc gcgagcgtcg
     4021 cctggtagac gcgcaccggg tgcacttcga ccaccttctg gccggtgcgc agccggtcgg
     4081 ttgcgccgcc cttgccggtg gcggtgattt cccagtagag acgctcgacg ccggccggcg
     4141 cttgcgccgg caccgggatc aacttcgcct cggctgccgc gagcgggacg gcctggctgc
     4201 ccttcgcgag cgtccggccc ttcgcgtcgc ccgcgggacg gtcgcgcagt tcccaggcga
     4261 gcgccgcggc gaccggctgc gcggtggtgt tcctcaacgt gaacatcgcc gtgaactcgt
     4321 cctgctcgcg caccaccggc ggcaggcccg aaaacagcat caggtcctgc gtcgtgcgca
     4381 ccgtggccat gccgtggccg aaccgcgcct ctccggcgac cgcgacggcg acgatccgga
     4441 acccggtgag cgcgtcgttc aacggcaccg ccaccaccgc ccgcccccgc tcatcgagcg
     4501 cgacgcgccc cttccacgca agcagcgtgt cgaacaactc gcgcgcgccg gcgcgcccgc
     4561 cgccgccgcc gggcggcgcc gctttcctgc cgaaatggcg cttgccgatg acctgcccct
     4621 gcgcggtcgc ggtctgcact tcgaccgggc ggttgccgag cagcgcatcg aggatgttcc
     4681 acgaggcgtt gtccatcagc tcgagcaggc cctcgtccac cgcggcgagc gcgatctccc
     4741 cgcccgcggc gggcttgccg tcgctgtcgg tgacctcgac ggcgatgcgc gccgtttcgc
     4801 gcacgcgaaa cgtttcgcgc tcgggcgtca ccttcacgtt gagcacgtaa tcgcgccgcc
     4861 cgaccttgat ttcggccatg ccgagcttgt aggcgggccg cgacaggtcg accagcgccg
     4921 tcgggcgcgt gtcgcgctcc accgggaccg cgtccaccag cccgaaccag tggccgacgc
     4981 ggtaagcggt gcgcttcaac cacgcgtacg gccccggcac ctccggatcg acgcgcccgc
     5041 gcaccgccag cgccgacacg aacacgttcg gcccgtagct gccggtcaac ggcacctcga
     5101 tcacgggcga tttgccgtcg agcgccacga ccttctgcgc cagcacgccc tcgcgctcga
     5161 ccgtcaccag cacggtggcc tcgcggaacg gcatgcgcac ctggaagcgc gcggtctcgc
     5221 ccggctcgta gcgcttcttt tccggcacga ggtcgatgcg atcgttgttg gagggctcga
     5281 accaccactc gccgtcgccc agcacccaca cgctcgacgt cgaaaaggtc tcgcgcccgc
     5341 cgtcgtcctt cgcgcgggcg accaggatca gctcgcccga ggtcccgggc ttcaccgagc
     5401 agaacatcag gccgcgcgcg tcggtcgtcc ccgagcagcc cgcgcccgcg cgccgcgtct
     5461 cggtggtcga atcgtaggcg tagaagccgc ccagcagccg gcggcggttg gagtaggtct
     5521 tgcgctcgta ggcctccacg ctgaccgcgc gcccggcgag cggcttgccg aggggatcga
     5581 gcgcgaccac ctgcgcgcgc acgcccttcc tgttcgccgc ccagccttcc ggcttgatgc
     5641 cgaggtacac gcccgccggg tgcagcgcca cccgcgcgga agccgcgagc agctcgccgt
     5701 tggggtcgct gtattccagc tcgaccagga ggctgccggg ccggtcgatc ggcggcagct
     5761 tgtcgatcac cacggacgcc gtgccggccg ggtcgagcac gagcgtgcgc gccgcggcgg
     5821 ggcccgcgcg ctccgccggc gccgggccgg catcgtcctc gtccggatcg aacgtcgccc
     5881 acaggtcttc gaccgagccg gtttgcatgc cctctttcac cgccgccccg ccgaaatgga
     5941 aatccgggta gtccgcgaac gacacgccgc gcggctccac gcggtagcgc accttcacgg
     6001 gcagaccgcc cgcggggccg ccggagaggt agctcaccgc ggcgtcgagc ttggcggcaa
     6061 cgggcttcac cagcggttcc ctgggcgcgg ccagcaccgc cttcatcagc ggcacgcgga
     6121 acgcctcgac gcggaactgt gcgttgccct ggatctcgcc gcgcccgggc cagtacaggc
     6181 gatagaagcc gagcttcgct tcggccggga tcttccatgt gctctccgcg attcccccgg
     6241 cgtcgaagcg cgcccccagc gggtacttct ggccgctgcc gatgtgctgg atttccgccg
     6301 ccgcgggcat ttcggttccc tgcggaacgc gaaagccggc gccggccggc acgcgcgcga
     6361 cgtgcttcat gctcacggtc tcgccggccc gcaacagcgt gcggtcgaac accgtatgga
     6421 tcgcgagcgg cttcaggcgg tacaggttcg ccgggaggtt gaaattccac ggccgtatgc
     6481 cttcgttcca ggaggaaaag gtgaaggaca ggtcctcgcc cagccgcgcg aaagcgatca
     6541 gttcgcgccg gtagtccttg cacgccggaa cctcggcctg cgccgggagc cggtcgcccg
     6601 cgtgcgcgat gccctgcgca tcggtcctgc cctcgaacca cagccgcccg gtgcagtcgc
     6661 gcaccgtgac gcgcgcgccg gcgacgggct tgccgcgatc gagcgacgtg acccagacca
     6721 gcgagctctc gcgcccgtgc ttgagatgga cggcgaggtt ggtgaccagc acgctggtcg
     6781 cggcgtaata gggcctgtcc tcgccgtgca gcgcgcgccc gagccgcggg ctcgcgagct
     6841 cgacgacgta gaaaccgggc cggggcagcg gaatacccac cacctcgaac gctttttcgc
     6901 cgccgggccg gggcagctgg aacgcgaccg ccttgtcgct gtcgaggatc accggcagct
     6961 cgccctcgtt cgggtagcgc ttcaacgccg cgcgcgcggg atggtcgcga tcgttttcgc
     7021 gaaaggcgcg gtagcgctcg atgatcctcg tctcgtcgtc cccgatgcgc gcggagcggc
     7081 cggcgatggg ccgttccgcc ccgctgggca gctcggccct gcgtccctgc agcggcgatt
     7141 cgacgttgcg taccgtcacc gggagcaccg ggtcggcgtt cagctcgagg atgccgaagc
     7201 gccccggaaa cttgacgagc ggggggttct cgtcggtggc ggtcgcgagc gggaacgacg
     7261 ccttgttttc cggctcgcgc ccggcgtcgt cgcggaagtc gcccggcacc ccgacggtat
     7321 agcgcgtctt ttccgggaac gggcccggga attcgaccga ctccacggtc ttcacgttgg
     7381 gctcgagcgt gggctcgaag gtcctgccgc cccccttcag ccggatgcgc gccgccgcct
     7441 cgcgcggcac cggcgcgttg aaatccagcg tgatgggcgc aaccggaagg cacgccgcct
     7501 cccggttcac gcgccggcac gtgagcttca cgaagaaggc cggccgcacc cggaacgcga
     7561 gccgctgcgg cgtctcccgc ggcagtcccg tcttcgattg cacgcccggc cccagcacca
     7621 ggccgatctc cgcgccggcg ggcagccgcc gcgcgcagcg cacgccgatc accggcagct
     7681 cgcgcaggcg cttgtcctcg attttgaagc tcgcggcagg gaagcggtgg cgtcccttga
     7741 agtaggcgtt gtagaggcgg taggcgcggt cgcggttggc cgccagcagc tcgcgcgtgt
     7801 cgccctcgga gagcagcctc agcgggatgc gctcgttgac gcccctcgcc tcgcaccacg
     7861 cgtcggagag gctcgccgcg tcgagcggcg catccagcgc gagcaggaac gcctgctcct
     7921 cgtccacgtg ctcctcgccc tcgtacggca gcgacgccat caccgcgggc ccgccggtgt
     7981 ggaagcggaa atcgcgccgg ccttcgaccg cctgcccgcc cggcgacttg aagtccgcct
     8041 tcagcatgaa cgtgcaggcc tggccggcgg gaagttccgc ctcgaagtcg tacgcccagt
     8101 tcttctggtc agcccagcgg ccgcggcctt tcagccgcgc ggggtcgccg tcgcagcgca
     8161 ccgtgaacgg atcgggcagc cgcgggtcgc cgaaagcgat catgggctcg ctgaagcgca
     8221 ccgccacctg gcgcacgccc ttggccgccc cctggggcgt gaacacgtcg acccgggggc
     8281 cggcccacgc ggcgcccgcc gccgcggcga acgcgatcgc cccgatcgcc tgattcagaa
     8341 ccgccgcacg caatcccatc ccgcaacccc cctggtggcg ccgtcacgcc ggcgttctcg
     8401 cggcgcgccc gccgaatatt tcgctccatt ctaccttcgc cgtcgcccgc atcagcacga
     8461 acaacgtcac gatcgcgccg agggtgatcg cgagcccggt cagccccttg aagaagaagg
     8521 cataggagaa cagcaccagg tacacgaatt gcgccgcgcc ggcggcgaag aacgcgaacc
     8581 ggttgccgaa catgacgcgc aggtagctca ccaccagcgc gagcgaggtc gccgcggcga
     8641 tcgcgaacgc ggcgtggatg gaaacgaggt ccgcgaggta ggcgaacagc aggtggaacg
     8701 agaagaacgc cgccgacagg aagaagaagt gcatgggatg cagctcgacg cgcttgacga
     8761 ccccgatcgt gagcatcacc acgatgaaaa agaacagcga aatcggcgcg aagcccgaga
     8821 tctggctggc gagcgggccg ggctgcagct tctcgggcat cagcatcgcg atgttgacgc
     8881 ccgtcaccag gcgccggaat tcccaggtca gcgcccagcc gccgccggcg cgctccttgc
     8941 gccccggcga gatgccgtcg tccgggaaat cgatcgcgtg gaaatcggtg cgcatggtga
     9001 gccggaagtc gcgcacctcg gacacgccgc tgccgaagct gtagctccag cgtcccagcc
     9061 cctggctgcg gtagcccgcg cgcacgttga cgcgctcgcc cggcgcgagc cgcacgctgc
     9121 cgacgatgcg gtcgtggctc ggtcgcgggg gcgcgatcca ttccttcccg gcgacttcga
     9181 gctgcaagtc gtcgaacacc gcgtttttcg cggggaacgg cagcgcgatc tcgatctcgc
     9241 gcgcggcgcc ggtcgtgttc accaccgcgt agacggcgga gaaatcgacc gcgtaggtcg
     9301 aataccagag caggcccttg cggcggtggt cgagcttcaa cgcgacatcg atcgcgctcg
     9361 aagcaagcgc gatcggatgc gtgacgcggg tctcgtccg
//
LOCUS       DBNGSG010000346         4147 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1471457, whole genome shotgun sequence.
ACCESSION   DBNGSG010000346 DBNGSG010000000
VERSION     DBNGSG010000346.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4147)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1471457"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..736)
                     /locus_tag="AC158Q_09015"
     CDS             complement(203..736)
                     /locus_tag="AC158Q_09015"
                     /inference="COORDINATES: protein motif:HMM:NF051921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7931 domain-containing protein"
                     /protein_id="HIR3339956.1"
                     /translation="MMGASEDKNPAMPLAGNLPNAEYRRFEGMREYEAIIDGLIPQTQ
                     RFIRVFDNTLSREWNTPRRYEALRQFLLANRTNRLLIVVHDAEPIERERPRLVDLVRL
                     FGTAVRIRQTQSPAKQIYDPFVVFDANHYVHRFHYRYLRAAQGTNDLIGTQQLLERYT
                     EIWEASALAVSAGASGL"
     gene            complement(821..1960)
                     /locus_tag="AC158Q_09020"
     CDS             complement(821..1960)
                     /locus_tag="AC158Q_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JmjC domain-containing protein"
                     /protein_id="HIR3339957.1"
                     /translation="MTKTPWASMSVAEFLRRHWQKRPLLARGALDQYSRLVRPGTLFE
                     LAGRDDLESRLVRRRGRRWEVRHGPFGRRELRGLPRSGWTLLVQGVNHALPAAQRLLE
                     QFSFIPYARLDDLMVSYAPEGGGVGPQFDDYDVFLLQGEGRRLWRVSRQRDLALVPGA
                     PLKILRRFRPAHEWVLEAGDMLYLPPRSAHDGVAVGGGCITYSIGFRAPDARELGARF
                     LDDLQDRLQLSGIYADPGLTPRRHPARLGDDMVRDAEKVLRRIRWGSGDVARFLGCHL
                     TEPKPHVVFSRPARPLTAWGFAARAASRGVRLALPTRMLFRGSMLFINGEACPAGAPA
                     TRLLARLADRRSLPPQARVGREAAELLYQWYRAGYIVLNGTRALV"
     gene            2068..2574
                     /locus_tag="AC158Q_09025"
     CDS             2068..2574
                     /locus_tag="AC158Q_09025"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR3339958.1"
                     /translation="MLPETLQTSTMLHPGQPAPGFDLPDADMNVVSLDSFRNRRNVVL
                     YFYPKDDTPGCTLEAIEFSEKEDEFGALKAVVLGVSMDDCLSHGAFRDKHGLCVQLLA
                     DADGEVCRRYGVLHDKELEGRKKTCIVRSTFIIDRKGVLKQALYGVNPRGHAQEILRL
                     LKGLGKWK"
     gene            2565..3044
                     /locus_tag="AC158Q_09030"
     CDS             2565..3044
                     /locus_tag="AC158Q_09030"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR3339959.1"
                     /translation="MEIKKDTVVSLTYELCDSDGGTIEKTDAPIEYLHGGYDGIFQQV
                     EKALAGKSAGESCRVRLEPDDAFGDYDAELVHLEPRDKFPDNIAVGMQFEGRGAESGA
                     TLIYTVTEIADNKVVVDGNHPLAGKTLEFSCTVTAVRAATQEELAHGHVHGAHGHHH"
     gene            complement(3126..4025)
                     /locus_tag="AC158Q_09035"
     CDS             complement(3126..4025)
                     /locus_tag="AC158Q_09035"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR3339960.1"
                     /translation="MIFKLIAAAALWLALCGGVGAAEFTFAAFGDTPYTQDEEARFPD
                     LIAEMNREPLAFVVHVGDFKSAHTRCSDELYLRRREWFDLFRHPFVLLPGDNEWADCR
                     RFTAGGYDPLERLRKLREIFFSGTESLGQRRIGLTRQTAGYPEHARWRLGDVLFATLN
                     VPGGANNARHDPVEFQSRGKAAERWLRESFRAAREEKLPAIAIFMHANPWASPTGRYF
                     GYRELLAALDAETRGFRGEVLLVHGDTHRHRVDRPAIHPDGGTPPANFLRVEVFGYPT
                     MNWVRIRVSDEAGRIRFTVTPGF"
BASE COUNT          773 a         1323 c         1380 g          671 t
ORIGIN      
        1 ggcagctgca cgatctgggt ctggatcctg gatattttca taaagtgcgt gattcgtgat
       61 tagtgatttg cgattcgtga aaaccgggag cgcgaactct atcagttgtc ggtgacaaac
      121 cacaacactg gttttgaatt accagccgcg tagtagtctg caatgcggac aggcgatcct
      181 tcgattcacg attcaccgtt tctcagagcc cgctcgcgcc ggcggacacc gcgagcgcgg
      241 acgcttccca gatctcggtg tagcgttcga gcagttgctg cgtgccgatc aggtcgttgg
      301 tgccctgggc tgcgcgcagg tagcggtaat ggaagcggtg cacgtaatga ttggcgtcga
      361 acacgacgaa cggatcgtag atctgctttg ccgggctctg cgtctggcga atcctgaccg
      421 cggtgccgaa cagccgcacg aggtccacga ggcgcgggcg ctcgcgctcg atcggctcgg
      481 cgtcgtgcac cacgatcagc aggcggttgg tgcggttcgc gagcaggaac tggcgcagcg
      541 cctcgtagcg tcggggcgta ttccactcgc gcgacagggt gttgtcgaag acacggatga
      601 agcgctgggt ctgcgggatc agaccgtcta tgatcgcctc gtactcgcgc atgccctcga
      661 agcgccggta ctcggcattc ggcaggttgc cggccagcgg catcgccggg tttttgtctt
      721 cacttgcacc catcattcaa cggtgtcctc tggcatgtca tcgcggaccc cgggcgcgtt
      781 gaacggcgac gcgattgtcc gcccgttcga acgcgctagg ttaaacgagc gcccgcgtcc
      841 cgtttaacac aatatatcca gcgcggtacc actggtaaag cagctccgcc gcttcgcgac
      901 cgacgcgggc ctgcggcggc agggagcggc gatcggccag ccgggccagc agccgggtcg
      961 cgggtgcgcc ggccgggcag gcctcgccgt tgatgaacag catgcttccg cggaacagca
     1021 tgcgcgtggg cagcgccagc cgcacgccgc ggctcgcggc gcgcgcggcg aacccccacg
     1081 cggtcagcgg ccgcgccgga cgcgagaaca ccacgtgggg cttgggttcg gtgagatggc
     1141 agccgagaaa ccgggccacg tcgccgctgc cccagcggat gcgccgcagc accttctccg
     1201 cgtccctcac catgtcgtcg ccgagccggg caggatggcg ccgtggcgtg agtcccgggt
     1261 ctgcgtagat tcccgacagc tgcaggcggt cctgcaggtc atccaggaat cgcgctccca
     1321 gttcccgggc atcgggcgcg cgaaagccga tcgaataggt aatgcagccg ccgccgaccg
     1381 cgacgccgtc gtgcgcgctg cgcggcggca ggtacagcat gtctcccgct tccagcaccc
     1441 attcgtgagc agggcggaaa cggcgcagga tcttcaatgg cgcgccgggc accagcgcga
     1501 ggtcgcgctg ccggctcacg cgccagagcc gccggccctc gccctgcagc aggaatacgt
     1561 catagtcgtc gaattggggg ccgacgccgc cgccctcggg cgcgtagctc accatcagat
     1621 cgtcgagccg cgcataaggg atgaaggaaa actgctcgag cagccgctgg gcggcgggca
     1681 gcgcgtggtt gacgccctgc accagcagcg tccagcccga ccggggcagc ccccggagtt
     1741 cgcgccggcc gaaaggtccg tgccgcacct cccagcgacg gccccggcgc cgcaccaggc
     1801 gcgactcgag gtcgtcgcgg ccggcgagct cgaaaagggt gcccggccgc accagccggg
     1861 aatactgatc gagggcgccg cgcgccagca gaggccgttt ttgccagtga cggcgcaaaa
     1921 actctgccac cgacatgctg gcccagggag tttttgtcat gcaaaacgat gataacgtac
     1981 gcgtaacatg ggtccgggaa aattgggccg caaaaacgct tgaccgcggc ggcggttgtt
     2041 cacaaaataa agtcagagcg cataaacatg cttccggaaa ccctgcagac gtcgaccatg
     2101 ctgcatcccg ggcagccggc accgggtttc gatttgcccg atgccgacat gaacgtggtc
     2161 agcctcgaca gcttccgcaa ccgccgtaac gtcgtgctct atttctatcc caaggacgac
     2221 acgccgggct gcaccctgga ggcgatcgaa ttcagcgaga aggaggacga gttcggcgcg
     2281 ctgaaggcgg tggtgctggg ggtcagcatg gacgattgcc tgtcgcacgg ggcgttccgc
     2341 gacaagcacg gcctttgcgt gcagctattg gccgacgcgg acggggaagt ctgccgccgc
     2401 tacggggtgc tgcacgacaa ggaactggag ggccgcaaga agacctgcat cgtgcgctcg
     2461 accttcatca tcgaccgcaa gggcgtgctg aagcaggcgc tctacggggt caacccgcgc
     2521 ggccacgcgc aggaaattct tcgattattg aaaggcctgg gcaagtggaa ataaagaagg
     2581 acactgtcgt ctctctcacc tacgagctgt gcgacagcga tggcggcacg atcgagaaaa
     2641 cggacgcccc gatcgagtac ctgcacggcg gttacgacgg catcttccag caggtggaga
     2701 aagcgctggc cggaaaatcc gcgggcgaga gctgccgcgt caggctcgag cccgacgacg
     2761 cgttcggcga ttatgacgcg gaactggtgc accttgagcc gcgcgacaaa tttcccgaca
     2821 atatcgcggt cggcatgcag ttcgaggggc gcggcgcgga atccggcgcg acgctgattt
     2881 acaccgtcac cgaaatcgcc gacaacaagg tggtggtgga cggcaaccat ccgctcgccg
     2941 gcaagacgct ggagttctcc tgcaccgtga ccgcggtacg ggcggcgacc caggaagaac
     3001 tcgcccacgg ccacgtgcat ggggcgcacg ggcaccatca ctgagatccg ttgctcgttt
     3061 ctcgttcttc gtttctcgtt gttttgacta ggacgagcaa cgagtaacga acaacgagtc
     3121 acttctcaaa aaccgggcgt caccgtgaac cggatgcgcc cggcttcgtc gctgacgcgt
     3181 atgcgcaccc agttcatcgt gggatagccg aacacctcca cgcgcagaaa attggcgggc
     3241 ggcgtgccgc cgtcggggtg gatggccggc cggtccacgc ggtgccggtg cgtgtcgccg
     3301 tgcaccagca gcacctcccc gcgaaagccg cgggtttccg cgtcgagcgc ggccagcagc
     3361 tcgcggtagc cgaagtagcg cccggtgggc gaggcccacg gattcgcgtg catgaaaatc
     3421 gcgatcgcgg gcagcttttc ctcgcgcgcg gcgcggaagg attccctcag ccatcgctcc
     3481 gccgccttgc cgcgcgactg gaactcgacg ggatcgtggc gcgcgttgtt ggcgccgccc
     3541 ggcacgttga gtgtcgcgaa caacacgtcg ccgaggcgcc agcgcgcgtg ctccgggtaa
     3601 ccggccgtct ggcgcgtgag gccgatacgg cgctggccca ggctttcggt tccgctgaag
     3661 aaaatctccc gcagcttgcg cagccgctcg aggggatcgt agcccccggc ggtgaaccgc
     3721 cggcaatccg cccattcgtt gtcgcccggc aggagcacga acgggtggcg gaaaaggtcg
     3781 aaccattccc ggcgccggag gtagagctcg tcgctgcagc gtgtgtgcgc cgacttgaaa
     3841 tcgccgacgt gcacgacgaa ggcgaggggc tcgcgattca tctccgcgat gagatcgggg
     3901 aagcgcgctt cttcgtcctg ggtgtagggc gtgtcgccga aggccgcgaa cgtgaactcc
     3961 gcggcgccga cgcctccgca cagtgcgagc cacagtgccg cggcggcgat aagcttaaaa
     4021 atcattcaat cgaaccgcca agacgctaag aaactcaaca tcgaaccgcc aaggccgcca
     4081 agaaaatcta tacaaaaaga attaacttgt ccgcgtgcgg cgcgcgcgag cgcatcgcta
     4141 gcgaagc
//
LOCUS       DBNGSG010000347         1874 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4358666, whole genome shotgun sequence.
ACCESSION   DBNGSG010000347 DBNGSG010000000
VERSION     DBNGSG010000347.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4358666"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="AC158Q_09040"
     CDS             complement(<1..187)
                     /locus_tag="AC158Q_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR3339961.1"
                     /translation="MSFPVHGLKAALTVLLCLVSWAANAADLKLWTGGAAPALALTDL
                     EGGSHRLADYRGRVVLIN"
     gene            complement(184..1722)
                     /gene="murJ"
                     /locus_tag="AC158Q_09045"
     CDS             complement(184..1722)
                     /gene="murJ"
                     /locus_tag="AC158Q_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HIR3339962.1"
                     /translation="MSLLKALATVSSMTLVSRILGFVRDLVIARVFGAGLATDAFFVA
                     FKLPNLLRRLFAEGAFSQAFVPILAEYKNRRGEQETRLLVDRVAAMLATALFIATLIG
                     VLAAPAIIYVTAPGFTATPGKFELTVDLLRVTFPYIFFVSLTALAGGILNTHSRFSIP
                     AVTPALLNLSFIGFALWGAPYFDPPVQALAWAVLAGGVAQLAFQVPFLARLGLLPRFR
                     FDFRDEGVRRILRQMGPAVFGVSIGQVSLVINVIFASFLVTGSVSWLYYADRLMEFPI
                     GLLGVALGTILLPSLSRHYADRSAQAYSELLDWGMRLTLLLAVPAAAALALLAVPLVA
                     TLFHYGAFGAEDVYATRRAVVAYSVGLVGLIMVKVLAPGFYARQDIRTPVRIAIVTLA
                     LTQLMNFVFVWPLQHAGLALAIALGACFNAALLLRRLRQSAIYTPQPGWPVFLFKLAI
                     AVYAMAALLWMVSGLDATWLAMGAAARAFNLAWVVLAGAATYFAALWLLGFRLADFSR
                     RSAE"
BASE COUNT          417 a          614 c          605 g          238 t
ORIGIN      
        1 agttgatcag gaccaccctg ccccgatagt cggccagtcg gtggctgccc ccttcgagat
       61 cggtcaacgc aagggcgggc gcagcaccgc ctgtccaaag cttcagatct gccgcgttcg
      121 ccgcccagct gaccaggcac aacaaaaccg ttagcgcagc cttcaggccg tgcaccggaa
      181 aactcattca gcgcttctcc tggaaaaatc ggcaagcctg aagcccagca gccatagcgc
      241 cgcgaagtag gtcgcggcgc ccgccaggac cacccaggcc aggttgaagg cgcgggctgc
      301 agcgcccatg gcgagccagg tggcatcgag gccggaaacc atccagagca acgcggccat
      361 ggcataaacc gcgatcgcga gcttgaacag gaataccggc cagcctggct gcggtgtata
      421 gatggcgctc tggcgcagcc tccgcagcag cagcgccgcg ttgaagcaag cgccgagcgc
      481 gatcgcgagc gcgagcccgg cgtgctgcag cggccagacg aacacaaagt tcatgagctg
      541 ggtcagggcc agcgtgacga tggcaatcct gaccggcgtc ctgatgtcct ggcgcgcgta
      601 gaacccgggc gccagcacct tgaccatgat cagcccgacc agccccacgc tgtaggcgac
      661 caccgcccgc cgcgtcgcgt acacgtcctc ggcgccgaat gcgccgtagt ggaagagcgt
      721 ggcgacgagc ggcacggcga gcagcgcaag cgccgcggcg gccggcaccg cgagcaacaa
      781 tgtcaaccgc attccccagt cgagcagttc ggaataggcc tgcgcggagc ggtcggcgta
      841 atgcctggac aggctcggca gcagtatcgt tcccagcgcg acgccgagca gcccgatcgg
      901 aaactccatc agccggtccg cgtaatagag ccaggacacg ctgccggtga cgaggaagga
      961 cgcgaagatc acgttgatca ccaggctcac ctggccgatt gagacgccga acaccgccgg
     1021 ccccatctgc cgcaggatgc ggcggacgcc ctcatccctg aaatcgaacc tgaaacgcgg
     1081 gagtagcccg agccgggcga gaaacggcac ctggaaagca agctgtgcca cgccgcccgc
     1141 cagcaccgcc cacgcgagcg cctgcaccgg cggatcgaag tacggagcgc cccacagcgc
     1201 aaaaccgatg aaggacaggt tgagcagcgc cggcgtgacg gccggaatcg aaaaacggct
     1261 gtgggtattg aggatgccgc cggcaagcgc cgtgagcgat acgaagaaaa tatacggaaa
     1321 cgtgacccgc agcaggtcca ccgtgagttc gaatttcccc ggcgtcgccg tgaaaccggg
     1381 tgccgtcacg tagataatcg caggcgcggc cagcacgccg atcagggtcg cgatgaaaag
     1441 ggcggtggcc agcatggcgg cgacacgatc gaccagcagc cgggtttcct gctcgccgcg
     1501 ccggttcttg tactcggcca ggatgggaac gaaggcctgg gagaacgcgc cttcggcaaa
     1561 caggcgccgc agcaggttgg gcagcttgaa ggcgacgaaa aacgcgtcag ttgccaaacc
     1621 ggctccgaac acgcgggcaa tgacgaggtc gcggacgaag ccgaggatgc gcgagacgag
     1681 cgtcatgctg ctcaccgtcg caagggcctt caacaggctc atgccgcccc gcgcgcctgc
     1741 atattacaaa cgttgcaaaa acccccgaac atcagtaaga tggcgccctt tttcgggaat
     1801 cagccccgga gtttatagca catggccaat atcgcatcag cacgcaagcg cgcacggcag
     1861 gcggagaagc gacg
//
LOCUS       DBNGSG010000348         3192 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2830924, whole genome shotgun sequence.
ACCESSION   DBNGSG010000348 DBNGSG010000000
VERSION     DBNGSG010000348.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2830924"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..684)
                     /locus_tag="AC158Q_09050"
     CDS             complement(178..684)
                     /locus_tag="AC158Q_09050"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3339963.1"
                     /translation="MPAPVYRLANPADAYEIAVMSRYLIEVGLRGWTWPPERVAKAIR
                     ARSTNVLVADVERNVVGFAIMDFGDTTAHLSLLAVKPTHQRCGIGVRLVAWQEEAALT
                     AGITAVSLELRTNNYAARAFYRMMGYKELSYIPGYYRGVETAVKMARDIRRVVPDLIK
                     EKPRNGQD"
     gene            complement(920..1489)
                     /gene="msrB"
                     /locus_tag="AC158Q_09055"
     CDS             complement(920..1489)
                     /gene="msrB"
                     /locus_tag="AC158Q_09055"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HIR3339964.1"
                     /translation="MTRRGWLQGMLAAGLWGALAGRGGAAQPKVMNMKDISELQKNWK
                     IYLAEGYKAPLPSEPLTLANAEWKKRLSPLAYSVLREEGTERAGTSPLNAEKREGVFV
                     CAGCDLPVYTSAMKYESGTGWPSFFTTIPGVFNTKKDYGLFLPRVEYHCKRCGGHHGH
                     VFEDGPPPTGQRWCNNGVALRFVPRTGKA"
     gene            complement(1640..2614)
                     /locus_tag="AC158Q_09060"
     CDS             complement(1640..2614)
                     /locus_tag="AC158Q_09060"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3339965.1"
                     /translation="MNFPTRSLVAAALAASALCGGSAMAADSYPARPIRLIVPFPPGG
                     SNDIVGRVIGAKLTERLGSQVVVDNRGGAAGAIGTEIAVNSQPDGYTLLVISAAHAYG
                     SSMRKTPFHPLKSITPIAMMGTGPTSLAVHPSVPVKSVKELIALAKAKPGQLNYATAG
                     VGSFQHLSSEMFRAMAGINAVHIPFKGGGPAMASVIAGQAQYVIGSLVQTLPFIKNGK
                     LKVLGTGGAKRDPVLPDVPTIAEAGVPGYEASNWWGIVGPAGLPAGPVKRISTEVSAV
                     LAQEEIRKWFISQGAQAVDRGPAEFRKWIASEIVKWSRVVNDAGLKGD"
     gene            complement(2762..>3192)
                     /locus_tag="AC158Q_09065"
     CDS             complement(2762..>3192)
                     /locus_tag="AC158Q_09065"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HIR3339966.1"
                     /translation="IPYKGTGQMITDLLGGQVPVMLASTAAVRPHVRGGRMRALGIST
                     VKRSPAMPEVVPIAESGVPGFNTASWVGLTGPRGMPRALIARLNSDIAAVIEGGDLRD
                     KLAAQGFVPEASTPQQFAAHVKDELGRFRKLVKAAGIKEE"
BASE COUNT          571 a         1069 c          986 g          566 t
ORIGIN      
        1 ccagatggcg gggggaaagc gctgacgcga gtttacgaat agacaggatt acccgaacgc
       61 atccaacacc cgtcaacacc gaaaatggac aggattaaca agatttacag gataagaaca
      121 aaaggaaaaa gaacagcgct tggaattcgc gctttgaagt ttgtatcctg aatcctgtca
      181 atcctgtcca tttctgggct tttccttgat gagatcgggc acgacgcggc ggatgtcgcg
      241 cgccatcttc accgcggtct ccaccccgcg atagtagccc gggatatagg acagctcctt
      301 gtagcccatc atgcggtaga aagcgcgcgc ggcgtagttg ttggtgcgca gctcgaggct
      361 cacggcggta atgccggcgg tcaacgccgc ttcttcctgc cacgccacca gccgcacgcc
      421 gatgccgcag cgctggtggg tcggcttgac tgcgagcaga gagaggtgcg ccgtggtatc
      481 gccgaaatcc atgatcgcga aaccgaccac attgcgctcg acgtccgcca ccagcacgtt
      541 ggtgctgcgc gcccggatcg ccttggccac ccgctccggc ggccaggtcc agccgcgcag
      601 cccgacctcg atcaggtagc gcgacatcac cgcgatctcg taggcatccg cgggattggc
      661 gagccggtaa accggggcgg gcatcggcaa actataccga aaattccggc actgtaaaca
      721 agcttccatg gtgtggacgc cggccgccaa tgaaaaggcg atagtggact ttccgtaccc
      781 agaccaaaaa agaaatcgga tggcggcccc gatggggcgt cattggcagt cgggcacgcg
      841 aagtcgccta tcgccagcaa aaaaaggcgg cgcatcgcgc cgccccggcc ttaggatccc
      901 gctcttcccg gaacgggtgt caggcctttc ccgtcctggg aacaaagcgc agggcaaccc
      961 cgttgttgca ccagcgctgc ccggtgggcg gcggtccgtc ctcgaacacg tgcccgtgat
     1021 gcccgccgca gcgcttgcag tggtactcga cgcgcggcag gaacagcccg taatccttct
     1081 tggtattgaa cacgccgggt atggtggtga agaagctcgg ccaccccgtg ccgctctcgt
     1141 atttcatggc ggacgtatag accgggaggt cgcagccggc gcagacgaac acgccctcgc
     1201 gcttctcggc gttcaagggg ctcgtgcctg cgcgctcggt gccctcctcg cgcagcaccg
     1261 agtaggccag cggagacagg cgctttttcc attccgcatt ggccagcgtg agcggttccg
     1321 agggcagcgg cgccttgtag ccttccgcca ggtaaatctt ccagttcttc tgcagttcgg
     1381 agatgtcctt catgttcatc accttcggct gcgcggcccc gccgcgcccg gcgagcgcgc
     1441 cccacagtcc cgccgccaac atgccctgca accatcctct gcgtgtcata acgtctcctc
     1501 gaaccatttt cccgcatccg cggacgccat catatacgga ccatcagccc tagcggatga
     1561 ggttgacgaa gcactgcagg ccagcgggca atcccgccac tgccccgatc atgagaaccc
     1621 ggcgatattc atgacagcac tagtcgccct tgagcccggc gtcgttgacc acccggctcc
     1681 atttgacgat ttccgaggcg atccatttgc ggaattccgc gggacccctg tcaacggcct
     1741 gcgcgccctg gctgatgaac cacttccgga tctcctcctg cgcgagaacg gcggacactt
     1801 ccgtgctgat ccgcttgacc gggccggcag gaagtccggc gggccccacg atgccccacc
     1861 aattgctcgc ttcatagccg ggaacgcccg cctccgcgat ggtcggcacg tcgggcaata
     1921 ccgggtcgcg ctttgcgccg ccggttccga gcactttcag cttgccgttc ttgatgaagg
     1981 gcagcgtctg caccagcgag ccgatgacgt actgggcctg ccccgctatg acggacgcca
     2041 tggcgggtcc gccgcccttg aacgggatgt gcacggcatt gatgcccgcc atggcccgga
     2101 acatctcgct ggagaggtgc tggaaactgc cgacgccggc cgtcgcgtag ttgagctggc
     2161 ccggcttggc cttcgcgagc gcaatcagct ccttcaccga tttcaccggg acgctgggat
     2221 gcaccgcgag cgatgtaggg ccggtgccca tcatggcgat cggcgtgatc gatttgagcg
     2281 gatgaaaagg cgtcttgcgc atcgagctgc cgtaagcatg ggcggccgag atcaccagca
     2341 gcgtgtagcc gtccggctga gaattcactg caatctcggt gccgatcgcg ccggccgcac
     2401 cgccccgatt gtcgacgacg acctggctgc cgagccgctc ggtcaacttc gccccgatca
     2461 cgcgccccac gatgtcgttg ctgcctcccg gcgggaacgg gacgatcagg cggatcgggc
     2521 gggcgggata gctgtcggcc gccatggcgc tgccgccgca taatgcactt gctgccagag
     2581 ccgcggcaac caggctgcgg gtgggaaaat tcatgggtgc tcctcgacgg ggaaactggg
     2641 gcgattctaa cagacgggtc atgcgctggc ggccagccgc gcggcgttga atcactgggc
     2701 gctcatgact gatccgacgg cgggcgatcc gcctttcgcc tttcgccttt cgactttcgc
     2761 cttactcttc cttgatgccc gccgccttca ccagcttgcg gaagcggccg agctcatcct
     2821 tgacgtgcgc ggcgaactgc tgcggcgtgc tcgcctccgg cacgaatccc tgcgctgcca
     2881 gcttgtcgcg caaatcaccg ccctcgatca cggcggcgat gtccgaattg agccgggcaa
     2941 tgagggcgcg cggcattccg cgcgggccgg tcagaccaac ccacgaggcc gtgttgaagc
     3001 ccggcacgcc cgactcggcg atgggaacga cctccggcat cgcgggcgag cgcttcacgg
     3061 tcgatatgcc aagcgcgcgc atcctcccgc cccgcacgtg cggccgcaca gccgccgtcg
     3121 aagccagcat gacgggcacc tggcctccca gcagatcggt gatcatctgg ccggtgccct
     3181 tgtacgggat gt
//
LOCUS       DBNGSG010000349         2770 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2660979, whole genome shotgun sequence.
ACCESSION   DBNGSG010000349 DBNGSG010000000
VERSION     DBNGSG010000349.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2660979"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(411..797)
                     /locus_tag="AC158Q_09070"
     CDS             complement(411..797)
                     /locus_tag="AC158Q_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011659979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIR3339967.1"
                     /translation="MIKHTGGCHCGRVRFEVLAPAKITVSECNCSICSKAGYLHLVVP
                     ADRFTLISGRETLTTYTFNTGTAQHLFCSVCGVKSFYVPRSHPDGFSVNARCLDPGTV
                     EDMTITQTDGRNWEKHYPAGRGEFRE"
     gene            complement(1066..1551)
                     /locus_tag="AC158Q_09075"
     CDS             complement(1066..1551)
                     /locus_tag="AC158Q_09075"
                     /inference="COORDINATES: protein motif:HMM:NF021996.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HIR3339968.1"
                     /translation="MKKNIIIFLVGAVLGTAGGFALGIFVYPYIFLADVVATERVENK
                     ASRKPVAGGEFIHANPRDPIHYGKGKVTVYQDLVHLGGDFEVGPGPKFHVYLVPEKNV
                     IASTAVEKTMFVDLGRLKAFRGSQNYPVPAGVDLSKFGSVVIWCEQFGVLISPAALKF
                     N"
     gene            complement(1594..2307)
                     /locus_tag="AC158Q_09080"
     CDS             complement(1594..2307)
                     /locus_tag="AC158Q_09080"
                     /inference="COORDINATES: protein motif:HMM:NF021604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HIR3339969.1"
                     /translation="MARYTNPQLRDRLAAEYVLGTLRGRARARFESLQRYDPELRRAV
                     AEWETRITPLAHAAPDVPPPARVWQAIVRRVAGASRDKGAWGPLALWRGLAVTSTAFV
                     LILAAFIGMTPRPEPPMEMVAVMNDDRGQPAMVVSWPPMKAMREPHIRIKVVQAHPVM
                     AAGTSWEMWMLPGGKAAPVSLGLITTYRDQVMKVKPALARRMGDAWGIAMSVEPAQGS
                     PTGAPTGPVIFKGQCVKIL"
     gene            complement(2403..>2770)
                     /locus_tag="AC158Q_09085"
     CDS             complement(2403..>2770)
                     /locus_tag="AC158Q_09085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3339970.1"
                     /translation="YAQAKSAPLTWMTSIVRNRCLDCLRRPRTEASGEEYDIAVEAWQ
                     DDSPGPMEQLMASTEAAALARCLQQLEAKQRQSIMLAFFNGLSHSELADHMKLPLGTV
                     KTWVRRGLERLKGCLGGAA"
BASE COUNT          469 a          847 c          836 g          618 t
ORIGIN      
        1 cagattagtt gacacttttc ccaagctgag tcggaacgtt ccgacattag ttgacaaatt
       61 aggtgacagt attggttgac accaagtcgc ctaacgcatg tcgaacttcc taattgtcgg
      121 ctaattagac actgaagccg atgcccttgt catccgcgct ccagaatcaa tcggttacct
      181 cggcaggact gcgtgaattc ctgaattagg ctgcgtggga gcgcgggaaa tccgaagaac
      241 caactgctcg aatctcgatg gagttgacgg ccttgttttc ttggcggcct tggcgtcttg
      301 gtggttcagt attttgatgt ttgaatcttg taaatcttgt taatcctgtc tattgatggt
      361 tttgacgggt ttgtgaatcg ttctgtcaat cctgtctatt acttgatttt ctactcgcgg
      421 aattcgcccc gcccggcggg gtagtgcttc tcccagttcc tgccgtcggt ctgagtgatc
      481 gtcatgtcct cgaccgtgcc ggggtcgagg cagcgcgcgt tgacgctgaa gccgtccggg
      541 tgcgagcgcg gcacgtagaa cgacttgacg ccgcagacag agcagaacag gtgctgggct
      601 gttcccgtgt tgaaggtata ggtcgtcagc gtctcgcgtc cgctgatcag cgtgaaccgg
      661 tcggccggca cgacgagatg cagatagccc gccttgctgc agattgaaca gttgcactcg
      721 gaaaccgtta tcttcgcggg ggccagcacc tcgaaccgca cccgcccgca atggcaaccg
      781 ccggtgtgct tgatcatgaa agctgttgcc ggttgtcttt gaagttatcg gcgtgtatct
      841 gcgtttatct gcggttccat tgttgttctt gtttttcttg acggccttgg cggttcagta
      901 ttttgttgtt tgaatcctgt aaatcttgtt tatccccttc ccccgacttg ggggaaggta
      961 ggatgggggc agctttctat taatgacttt gacgggttct tgaatcgttc tgtaaatcct
     1021 gtccattact tgttttatcg gtgtgtatcg gcgttcatcg gcggttcaat tgaatttaag
     1081 cgctgccggc gagatcagca ccccgaactg ctcgcaccag atcacgacgc tcccgaactt
     1141 cgacaggtcc acgcccgccg gcacgggata gttctggctg cccctgaagg ccttcagccg
     1201 cccgaggtcc acgaacatgg ttttctcgac ggcggtggag gcaatcacgt tcttctccgg
     1261 caccaggtag acgtggaact tgggtcccgg cccgacttcg aaatcaccgc ccaggtgcac
     1321 caggtcctgg tacaccgtca ccttcccctt gccgtagtga atgggatccc tgggattggc
     1381 gtggatgaac tcgccgcccg ccaccggctt gcgcgacgcc ttgttctcca cccgttccgt
     1441 ggcgacgacg tccgccagga agatataagg gtagacgaag atgcccaatg caaatccccc
     1501 cgcggttccc agcaccgcac caaccaggaa aatgatgatg ttttttttca cggtctggcg
     1561 cctttatctg cgttcatcgg cgttcatcgg cgactatagg attttcacac attggccctt
     1621 gaagatcacc ggccccgtcg gcgccccggt gggcgagccc tgcgcaggct cgacgctcat
     1681 cgcgatgccc caggcatcgc ccatcctgcg ggcgagagcg ggtttcacct tcatcacctg
     1741 gtcccggtag gtggtgatga gtcccagtga caccggcgcg gccttgccgc cgggcagcat
     1801 ccacatttcc cacgacgttc ccgccgccat gacgggatgc gcctggacga ccttgatgcg
     1861 gatatgcggc tcgcgcatcg ccttcatcgg cggccacgac accaccatcg cgggctggcc
     1921 gcggtcgtcg ttcatgaccg cgaccatctc catcggcggc tcgggccgcg gcgtcatgcc
     1981 gatgaacgcg gcgaggatca ggacgaaagc ggtgctggtg acggcgagcc cgcgccacaa
     2041 ggcgaggggg ccccaggcgc ccttgtcgcg cgaagcgccg gccacgcgcc gcacgatggc
     2101 ctgccacacg cgcgccggcg gcggaacgtc gggcgcggcg tgggcgaggg gcgtgatgcg
     2161 cgtctcccac tcggcgacgg ctctccgcaa ttcgggatcg tagcgctgca gggactcgaa
     2221 gcgcgcgcgg gcgcgcccgc gcagcgtgcc gagcacgtac tccgcggcga ggcggtcacg
     2281 cagttgcggg ttcgtgtatc tggccatgcg cattaagtca aaaggcacaa ggcaaaaggg
     2341 ttcggattcg cgccgttcct tgtgccttgt gccttgtgac ttgtgccttc atctctatcc
     2401 cctcaggccg ccccgccgag acaacccttg aggcgctcga ggccgcgccg cacccaggtc
     2461 ttgaccgtcc cgagcggcag cttcatgtga tccgcgagct ccgagtggga gaggccgttg
     2521 aagaacgcga gcatgatgct ctggcgctgc ttcgcttcca gctgctgcag gcagcgcgca
     2581 agcgccgccg cttcggttga tgccatcaac tgctccatcg gccccggcga gtcgtcctgc
     2641 caggcctcga ccgcgatgtc gtattcctcg ccgctcgcct cggtgcgcgg ccggcgcagg
     2701 cagtcgaggc agcggtttct cacgatggag gtcatccagg tgagcggcgc gctcttcgcc
     2761 tgcgcatagt
//
LOCUS       DBNGSG010000350         2617 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2661252, whole genome shotgun sequence.
ACCESSION   DBNGSG010000350 DBNGSG010000000
VERSION     DBNGSG010000350.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2661252"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..1127
                     /locus_tag="AC158Q_09090"
     CDS             165..1127
                     /locus_tag="AC158Q_09090"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3339971.1"
                     /translation="MRHQTAVLLVCGVALLAAAPAAHAQGWPAKPLRIIAPVPAGAGA
                     DGVARDVAAGLTERLKQSVVVENRPGAAGNIGMEAVARSAPDGYTLLLAVTSLVSNPH
                     LYRLSYDPLNDFAPVTLLTRSHFVLVAHPTLPATTVREVLALAKARPGIVTCAHASGS
                     LHIACAWLKTAGRADINLVPYKGGIQALHDVIGGHVDMTFGVVQTVVPHAQSNRLRVL
                     ATTNRRRGSGPFGGSPTIAETLPGFVLLSWQGLLVPAGTPREIITRLSVEIGAVLEQQ
                     DVKKRILDGGLEPAHGTPDAFAETIRRDYALYEKIIRETGMRVQ"
     gene            1225..1425
                     /locus_tag="AC158Q_09095"
     CDS             1225..1425
                     /locus_tag="AC158Q_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339972.1"
                     /translation="MTDDQFKTLRSIQFAQLGLLQSIEANIRLIAQQQKAPSGGLGVN
                     WRRTRELLDGVRNWKGRSAEGS"
     gene            1745..>2617
                     /locus_tag="AC158Q_09100"
     CDS             1745..>2617
                     /locus_tag="AC158Q_09100"
                     /inference="COORDINATES: protein motif:HMM:NF012573.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HIR3339973.1"
                     /translation="MATFLGTSSPDTLSGGSGNDIIIGYDTLAGDVGVADNDSLRGFG
                     GADSILGGTGNDTLFGGDGSGADTLDGNDTLGGGDGNDSLSGGFGADLLLGGFGNDTA
                     LGGDGDDGVAGSLGNDLLFGGAGNDAMGGGGDADSLQGDDGRDSLDGGGGDDSLLGGL
                     GDDLLLGGSGDSVIGFGDDTLHGGQGNDTLDGGMGRDSLVGGAGNDTLYGGALIESLV
                     GFGDDTLDGGDGNDVLDAGFGDDSLIGGIGDDVLYGGADTIAGIGNDTLDGGDGNDSL
                     AGGNGNDSLAGGTGN"
BASE COUNT          434 a          895 c          928 g          360 t
ORIGIN      
        1 ggccagcacg tgccgcgcaa attcatcgcc gtgagcgagc tgccgcgcaa ccagatgggg
       61 aaagtcgtcc ggcacgagct ggcggcgcgc gtcgcatccg aaagcccgcc cgcgggtgcg
      121 cccgccccgg ccggtagcgg tcgggggaga cggggaggcc catcatgaga catcaaacgg
      181 ctgtcctcct ggtatgcggt gtcgcgctgc tcgccgcggc gccggccgcg cacgcgcagg
      241 gctggccggc gaagccgctg cgcatcatcg ccccggtgcc ggcgggcgcg ggcgcggatg
      301 gcgtggcgcg cgacgttgcc gccgggctca ccgagcggct gaagcagtcg gtcgtcgtgg
      361 agaaccgccc gggcgcggcc gggaatatcg gcatggaggc cgtcgcgcgc tcggcccccg
      421 acggctacac cctcctgctc gcggtgacgt ccctcgtctc caacccgcac ctctaccggc
      481 tcagctacga cccgctcaac gacttcgcgc cggtgacgct gctcacccgc tcccacttcg
      541 tgctggtggc gcatccgacg ctgccggcga cgacggtgcg cgaggtcctg gcgctcgcca
      601 aggcgcggcc cgggatcgtg acctgcgccc acgcgagcgg ctccctgcac atcgcctgcg
      661 cctggctcaa gacggcaggc cgcgcggaca tcaacctcgt gccgtacaag ggcggcatcc
      721 aggcgctgca cgacgtcatc ggcgggcacg tggacatgac gttcggggtg gtgcagaccg
      781 tggtgcccca cgcgcaatcc aaccgactca gggtcctggc gacgaccaac cggcggcgcg
      841 gcagcgggcc tttcggcggt tcaccgacga tcgcggagac gctgcccggg ttcgtgctgt
      901 tgagctggca gggcttgctg gtgccggccg gcacgccgcg ggagatcatc acgcggctca
      961 gcgtagagat cggcgccgtg ctcgagcagc aggacgtgaa aaaacgcata ctggacggcg
     1021 ggctggaacc ggcccacggc acgcctgatg cgttcgctga aaccatacgg cgcgattacg
     1081 ccctgtatga aaaaatcatc cgcgagacgg gcatgcgcgt tcagtgaacg gaccggccgc
     1141 gggcagcgcc ccggaggcac gtgatatttt gagataagct ttaaccccgt tttccttttc
     1201 cgaccaacca gggagcatcc atccatgacc gacgaccaat tcaaaacctt gagatccatc
     1261 cagttcgcgc agctcggcct gctgcaatcc atcgaggcca atatccggct gatcgcgcaa
     1321 cagcagaagg cgcccagcgg gggtctcggc gtcaattgga ggcgcactcg cgagctcctc
     1381 gacggcgtgc gcaactggaa gggccgcagc gcggaagggt cgtgacggcg gccggccgcc
     1441 gcggggcctc cagattggcg cgtgccgaag gcgcccggct tcgccgctcc cgccacggtc
     1501 gggatatcgt gaacaaaatc acgtttcctt ccccgcggcg ccgccagcgg cggtagcggg
     1561 gcgggaccgc cgaccgtgcc ggccattcca gggggcgaag cgccacgttg cccccgtcac
     1621 aaaagcctgc ctacaatgtg aaaacccgcg ggcgccggtc cggacccggg cgggatgtcc
     1681 ggccgccgcg cggatgcccc ggagaacaaa aatcaacagg taacaccaca gggaagggcg
     1741 tcacatggct acgttccttg gcacttccag tcccgacacc ctgtcgggcg gttccggcaa
     1801 cgacatcatc atcggttacg acacgctcgc gggcgatgtc ggcgtggccg acaacgattc
     1861 gctccggggt ttcggcggag cggactcgat cctcggcggc accggcaacg acacgctctt
     1921 cggcggcgac ggcagcggcg ccgatacgct cgacggcaac gacacgctcg gcggcgggga
     1981 tggcaatgac tcgctctccg gcggttttgg cgcggatctc ctgctcggcg gttttggcaa
     2041 cgacacggcg ctcggcggcg acggcgacga cggggtcgct gggagcctgg gcaacgacct
     2101 cctgttcgga ggcgccggca acgatgccat gggcggcggc ggcgacgccg acagcctgca
     2161 gggcgacgac ggccgcgaca gtctcgatgg cggcggcggc gacgacagcc tgctcggcgg
     2221 cctcggcgat gatttgctcc tgggcggcag cggggacagc gtcatcggct tcggcgacga
     2281 taccctgcat ggcggccagg gcaacgacac gctcgatggc ggaatgggcc gtgacagcct
     2341 cgtcggaggt gccggcaacg acacgcttta cggcggcgcg ctgatcgaga gcctcgtcgg
     2401 cttcggcgac gacacgctcg acggcggcga cggtaatgat gtgctggacg ccggcttcgg
     2461 cgacgacagc ctgataggcg gcatcggcga cgacgtgctg tacggcggcg ccgataccat
     2521 cgcggggatc ggcaacgaca cgctcgacgg cggcgacggc aacgacagcc tggcgggcgg
     2581 caacggcaac gacagcctgg cgggcggcac tggcaac
//
LOCUS       DBNGSG010000351         3999 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1812524, whole genome shotgun sequence.
ACCESSION   DBNGSG010000351 DBNGSG010000000
VERSION     DBNGSG010000351.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1812524"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1312
                     /locus_tag="AC158Q_09105"
     CDS             <1..1312
                     /locus_tag="AC158Q_09105"
                     /inference="COORDINATES: protein motif:HMM:NF013109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HIR3339974.1"
                     /translation="FFGMEIPPGASFAGTWYVFVTGLDRASPYAVTVGTVTLLTGILL
                     RRIAPRFPYMIVAMIAGGAIAALLNAAFGEARTGIAMVGALPGSLPEFSVPDPSADAI
                     RKTLPVALAVTILALTEAVSIARAIAVKSGQRIDGNQEFIGQGLSNIAGSFFSAYASS
                     GSFNRSGVNFEAGARTPLAAMLAGLMLVPILFAVAPLIAHLPLAVMAAILFLVAYGLV
                     DFKYIARTLKTSRQETAVMLLTFLAAIFASLEFAIYTGVLLSLMLYLTRTSRPLVLDV
                     KPDPAPDSYHYTADSGLPDCPQLKMLRVNGSLFFGAVDHIQNHLERVDETDPRQKHVL
                     IVASGINFVDMAGAEMLAREAQRRRRMGGGLYFYRMKDVVRLLLARGGYLGDIGEDNL
                     FPVKSRPVAAIYRKLDSGICRNCKARIFRECHVSLPNGDPVQAR"
     gene            complement(1328..2395)
                     /locus_tag="AC158Q_09110"
     CDS             complement(1328..2395)
                     /locus_tag="AC158Q_09110"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR3339975.1"
                     /translation="MEHATIQFIDLQYGNVLALLAIGFVGGLVSGFIGSGGAFVLTPA
                     MMSMGVPGVVAVASNLCHKFPKALVGAYRRNKFGQVDVKLGLVMAVSAMAGVWVGADI
                     QNQIKLDYGEAGSNLYVSLAFVVVLTIVGAFVLHDAIRVYRSGDTDAQEKPSPLALWV
                     QSVEIPGTMMHFRTANIRVSALFTLPLGFATGLLASIIAVGGFIGVPGMMYVLGAPGL
                     VASATELVIAFVMGFGGTIKYSLHGLVDIRLALILLAGSLFGVQLGAIGTTFVKPFVI
                     KVVMAAIMLIVAVSRGLVVPVYLGQLGQMELAIETGARLQSASFAFLLLALCTGGIII
                     IASMLRGHRAGAAPLREVSSD"
     gene            complement(2399..2629)
                     /locus_tag="AC158Q_09115"
     CDS             complement(2399..2629)
                     /locus_tag="AC158Q_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339976.1"
                     /translation="MKKSKPYLQLIASGMASGALYLSLYAYEREILAAFTRTDGWFPA
                     LPVLAAFVFSFAHGAFTGYFWEVLGIRARRKQ"
     gene            2703..3254
                     /locus_tag="AC158Q_09120"
     CDS             2703..3254
                     /locus_tag="AC158Q_09120"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011544630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HIR3339977.1"
                     /translation="MGNRLTRIYTRTGDGGTTGLADGARVSKDAPRIEAIGAVDELNS
                     ALGVLLAETLPDAARDCLVDVQNDLFDLGGELSVPGHAIMSKAHVERLERDLDRFNAG
                     LPALKDFILPAGSRAAALAHLARTVCRRAERRLVTLARKQKIAPALRAYVNRLSDLLF
                     VLARELNRAAGKPDVLWRQGKNR"
     gene            3352..3927
                     /locus_tag="AC158Q_09125"
     CDS             3352..3927
                     /locus_tag="AC158Q_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3339978.1"
                     /translation="MSTSSPNDLAGRLAGLLAQCALKNQRAFAELYQLTAAKLYGVAL
                     RILRRQDWAEEVLQECYVNIWNHAGNYAQAKSAPLTWMTSIVRNRCLDCLRRPRTEAS
                     GEEYDIAVEAWQDDSPGPMEQLMASTEAAALARCLQQLEAKQRQSIMLAFFNGLSHSE
                     LASHMKQPLGTVKTWVRRGLERLKTCLGAAT"
BASE COUNT          741 a         1359 c         1274 g          625 t
ORIGIN      
        1 tttcttcggc atggagatcc cgcccggtgc ctcgttcgcc ggcacctggt acgtgttcgt
       61 caccgggctg gaccgcgcca gcccttacgc cgtgacggtc gggaccgtca cgctgctcac
      121 cggcatattg ctccgcagga tcgcgccgcg ctttccttac atgatcgtcg ccatgatcgc
      181 gggcggggcc atcgcggcgt tgctcaacgc cgcgttcggg gaggccagga ccggcattgc
      241 gatggtggga gcgctgcccg gctcgcttcc cgagttctcc gtcccggatc cgtccgccga
      301 cgcgatccgc aagaccctgc cggtcgcgct cgcggtaacg atactcgccc tcaccgaggc
      361 ggtttcgatc gcgcgcgcca tcgcagtgaa gtccggacag cgcatagacg gcaaccagga
      421 gttcatcggg caggggctgt ccaatatcgc gggcagcttc ttctcggctt atgcctcgag
      481 cggctccttc aaccgcagcg gggtgaactt cgaggcgggc gcgcgcacgc cgctggcggc
      541 catgctcgcc gggctcatgc tcgtgccgat cctgttcgcc gtcgcgccgc tcatcgcgca
      601 cctgccgctc gccgtcatgg cggcaatcct gttcctcgtg gcctacggcc ttgtcgactt
      661 caagtacatc gcgaggacgc tcaagaccag ccgccaggaa acggcggtca tgctgctgac
      721 gttcctcgcg gcgatcttcg ccagcctcga gttcgcgatc tacacgggcg tgctgctgtc
      781 gctgatgctg tacctcacgc gcacgtcgcg gccgctggtg ctcgacgtca agcccgaccc
      841 ggcgcccgac agctatcact acaccgccga cagcgggctg cccgactgcc cgcaactcaa
      901 gatgctgcgc gtgaacggct cgctcttctt cggcgccgtg gaccacatcc agaaccacct
      961 ggaacgggtg gacgagaccg atccacggca gaagcacgtg ctgatcgtcg cgagcggaat
     1021 caacttcgtg gacatggccg gcgcggaaat gctggcgcgg gaagcgcagc ggcggcgacg
     1081 catgggcgga ggtctctact tctaccgcat gaaggacgtc gtgaggttac tgctcgcgcg
     1141 cggcggctat ctcggcgaca tcggcgagga caacctgttc ccggtgaaaa gccgcccggt
     1201 ggcggcgatc taccgcaagc tcgattccgg gatctgccgc aactgcaagg cccgaatttt
     1261 ccgcgagtgc cacgtcagcc tgcccaacgg ggacccggtg caggcgagat aagcgcgccg
     1321 ccgcccccta gtcgctggag acttccctga gcggcgcggc cccggcccgg tgcccgcgca
     1381 gcatggaagc gatgatgatg attcccccgg tgcacagcgc gagcagcagg aacgcgaaac
     1441 tcgccgactg caatcgggcc ccggtctcaa ttgccagctc catctggccg agttgcccca
     1501 ggtagacggg caccaccagg ccgcggctga ccgcaacgat cagcatgatg gcggccatga
     1561 cgaccttgat cacgaagggt tttacgaaag tggtgccgat cgcgccgagc tggacaccga
     1621 acagagagcc cgcaagcagt atgagtgcga ggcgaatgtc caccaggccg tgcagggagt
     1681 acttgatggt gccgccgaac cccatcacga atgcgatgac cagctccgtc gcgctggcaa
     1741 cgaggccggg cgcgcccagg acgtacatca tgcccggcac gccgatgaag ccgccgaccg
     1801 cgatgatgga ggcaagcaag ccggtggcaa agccgagcgg cagcgtgaac agggcggaca
     1861 cccggatgtt cgccgtcctg aaatgcatca tggtccccgg aatttcgacc gactgcaccc
     1921 agagcgcgag cggcgatggc ttctcctgcg cgtcggtatc ccccgagcgg tagaccctga
     1981 tggcatcgtg cagcacgaac gcgccgacga tggtcagcac gacgacgaac gcgaggctca
     2041 cgtacaggtt cgagccggct tctccgtagt ccagcttgat ctggttctgg atatccgcgc
     2101 cgacccacac gccggccatc gcggagactg ccatcacgag gccaagcttg acatccacct
     2161 gcccgaactt gttgcgccgg taggcgccga ccagcgcctt ggggaacttg tggcacaggt
     2221 tgctcgccac cgcgacgacc ccgggcacgc ccatgctcat catggccggc gtcagcacga
     2281 aagcgccgcc cgacccgatg aacccgctca ccagcccgcc gacaaacccg attgcgagca
     2341 gggcaagcac gttgccgtac tgcaggtcga taaactggat cgtcgcatgc tccatggctt
     2401 actgtttccg ccgcgcccgg atgccgagca cctcccagaa gtaaccggtg aaggcgccgt
     2461 gcgcgaatga aaacacgaag gccgcgagga ccggcaacgc cgggaaccag ccgtcggttc
     2521 gcgtgaaggc cgccaggatc tcccgttcgt acgcgtagag cgacagatac agcgcgccgg
     2581 acgccattcc cgacgcaatc aattgcaggt agggtttcga ctttttcacg cggcgagcct
     2641 tgacgcgaat catagcaaag gcgcggtccc cgcacccaac cgccgctata attgccgttc
     2701 acatgggcaa tcgcctcacc aggatctaca cgcgcaccgg cgacggcggc acgacgggcc
     2761 tcgcggacgg cgcgcgcgta tcgaaggatg cgccgcgcat cgaggcgatc ggcgcggtgg
     2821 atgagctgaa cagcgcgctc ggcgtgctgc tggccgagac gctgccggac gcggcgcgcg
     2881 attgcctcgt cgacgtccag aacgacctgt tcgacctcgg cggcgaactc tccgtgcccg
     2941 ggcacgcaat catgagcaag gcgcacgtgg agcggctcga acgcgatctt gaccgcttca
     3001 atgccgggct gcccgcgctc aaggatttca tcctgccagc gggctcgcgc gcggccgcgc
     3061 tggcccacct ggcccgcacc gtctgccgcc gcgccgagcg caggctcgtc accctcgcgc
     3121 gcaagcagaa gatcgcgccc gcgctgcggg cttacgtcaa ccggctctcc gacctgctgt
     3181 tcgtgctggc gcgggaactc aatcgcgcag ccggaaagcc ggacgtgctg tggcggcagg
     3241 gaaagaacag atgaaccaag gcacaggtca caaggcacaa ggcacaagga acggcgcgaa
     3301 aaccggcccc ttttgccttt tgcctggtgc cttgtgcctt ccggccggcg catgagcacg
     3361 tcctccccca acgaccttgc cggccggctc gccggcctgc tggcccagtg cgccctcaaa
     3421 aaccagcgtg cattcgcgga gttatatcag ctcaccgcgg cgaaactata tggggtcgcg
     3481 ctgcgtatat tgaggaggca ggactgggcc gaagaggtat tgcaggaatg ctacgtcaac
     3541 atctggaacc acgccgggaa ctatgcgcag gcgaagagcg cgccgctcac ctggatgacc
     3601 tccatcgtga gaaaccgctg cctcgactgc ctgcgccggc cgcgcaccga ggcgagtggc
     3661 gaggaatacg acatcgcggt cgaggcctgg caggacgact cgccggggcc gatggagcag
     3721 ttgatggcat cgaccgaagc ggcggcgctt gcgcgctgcc tgcagcagct ggaagcgaag
     3781 cagcgccaga gcatcatgct cgccttcttc aacggcctga gccattcgga actggcgagc
     3841 cacatgaagc agccgctcgg cacggtcaag acctgggtgc gccgcggcct cgagcgcctc
     3901 aagacctgcc ttggcgctgc aacctgaagg atgaagacag aaggatgaag gcagaagtaa
     3961 caccgatcac cgatcactgg tcaccgatca cgctttatt
//
LOCUS       DBNGSG010000352         4651 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_965114, whole genome shotgun sequence.
ACCESSION   DBNGSG010000352 DBNGSG010000000
VERSION     DBNGSG010000352.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4651)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_965114"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..905)
                     /locus_tag="AC158Q_09130"
     CDS             complement(177..905)
                     /locus_tag="AC158Q_09130"
                     /inference="COORDINATES: protein motif:HMM:NF024921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR3339979.1"
                     /translation="MNPQIRAAGPSAIKVLTSHSSFSVLDALAPEFERAAGQEVAIEA
                     DSAKTMLARIKGGETGDVLVIGKSGADELAQLGIIVPGSSRPFARARVGVAVRAGAPR
                     PDISSVEAFKRSMLSAKSIAHTVNGASGMYVPVLMERLGIADKMKPKTVTRPGGYIAR
                     VVAAGEAEIAIQQIVELLAVPGVDLVGPLPDEIQKVFETTAAIFTSSNRPAAAEALLR
                     FLLAPGAAGVFGDKGLELVVAGNA"
     gene            1117..2022
                     /locus_tag="AC158Q_09135"
     CDS             1117..2022
                     /locus_tag="AC158Q_09135"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3339980.1"
                     /translation="MHRAFLAAITVAVTLAAAPAGAQDKPAAPAAKPPSFSTTRIEGT
                     ENVYAFTFGNRVSMFVVTTAGVIATDPGGYGQPQRVPTYLDEIRKVTDQPVKYLIYSH
                     HHYDHIAGGQPFKDAGARIVAHKRAKERLMMIKDPATPLPDEVFDKKRVIKLGNTTLE
                     LTYHGLNHSDSTIAMRLPREKILFVVDILPVGSVPGRGMIDFYPLEAEKSIEEILKLD
                     WDRLIPGHPGQPGGRLGTKADAQAQLAFLRDASETVKAAAREGKCWDRVEKELKLPQY
                     ESWPGYQNNLQFVLRRYCGLWGRGT"
     gene            complement(2196..2657)
                     /locus_tag="AC158Q_09140"
     CDS             complement(2196..2657)
                     /locus_tag="AC158Q_09140"
                     /inference="COORDINATES: protein motif:HMM:NF012787.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(2520..2522),aa:Sec)
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HIR3339981.1"
                     /translation="MDIKEGAAAPDFALPAHNTDQKISLSSFKGRPVVVAFMPFAFTG
                     GUTNQVNGFRANYEEFKKLNCEILQISADPAPSLKGWSEKLGGVPFPLLSDYWPHGAV
                     GKAYGIFNEERGMDKRAAYVVDAKGIVRYAKVYPQGTIPESPELLAELRKL"
     gene            complement(2828..3064)
                     /locus_tag="AC158Q_09145"
     CDS             complement(2828..3064)
                     /locus_tag="AC158Q_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339982.1"
                     /translation="MFAIICKADGFPVSRQVGNNTPDMVVTWTSQDKARAFLAAKGLE
                     TEYQVVALTDDSLNSMAKALGCQADAIAFDAYPG"
     gene            complement(3192..3803)
                     /locus_tag="AC158Q_09150"
     CDS             complement(3192..3803)
                     /locus_tag="AC158Q_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-disaccharide hydrolase"
                     /protein_id="HIR3339983.1"
                     /translation="MKRLSIICMGLLVTALTVIGCATTSGSGWVTLIDGEKGLENFNR
                     IGDVNWRAEGGAIVGDKGKGGHLVTKNSYKDFEIRAEFWAETTTNSGIFVRASDPNKI
                     GAANAYEVNIFDMRPGQEYATGGIVGFAMVPVPIIHKAGGKWNTLEIYAKGSEVTVKF
                     NGAVTVSLQDKKFASGPFSLQFGNRGKEPGGAIKWRKVQVRTL"
     gene            4005..>4651
                     /locus_tag="AC158Q_09155"
     CDS             4005..>4651
                     /locus_tag="AC158Q_09155"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3339984.1"
                     /translation="MKLPITGALLCAWSVVSMPAALAQTAKYPDRAIRLISPFPPGGT
                     VDVNARILAPAVSKSLGQQVVVDNRAGASGIIGTAEAARAKPDGYSLLINTTPLVTNT
                     LMYSKVPYNLLKDFEPISILSTTPSFLSVHPSVPVKTVREMIQLAKARPGALNYAAAG
                     VGTNPHIAGELLNHLAKIKLLAVHYKGGGPATVATLSGETQLYFVNAIPEALPHI"
BASE COUNT          861 a         1471 c         1437 g          882 t
ORIGIN      
        1 tggtcagcag catggcagtg aggaacccgt ccctggacgg gtcgcccgcg atcagggctg
       61 ccgccagcgg cgccagcatc gtaccgccga agatcaccag aaccaggccg aagatacgca
      121 ggacggagcc ggcctggcgg gcgcctgatg aggggagggc gagtgtttgg ggcatgctat
      181 gcgttgcccg cgaccacgag ttcgagtcct ttatctccga atactccggc cgcgcccggc
      241 gccaggagaa accggagcag cgcttccgcc gccgccggcc ggtttgaaga cgtgaaaatc
      301 gcggccgttg tctcgaatac cttctggatc tcgtccggca gcggccccac gagatcgact
      361 ccgggcaccg ccagcaattc cacgatctgc tggatggcga tctcggcctc gcccgccgcc
      421 acgacccgcg cgatgtagcc gcccggccgc gtgacggtct tgggcttcat cttatccgcg
      481 atgccgaggc gctccatcag gactggaacg tacatcccgc tcgcgccgtt cacggtgtgc
      541 gcgatcgatt tcgcggagag catcgagcgt ttgaaggctt cgaccgacga gatgtcgggg
      601 cgtggagcgc cggcgcgaac ggcaacaccg acacgggcgc gggcaaaggg gcggctgctg
      661 ccgggcacga tgatgcccaa ctgcgccagc tcatcggccc cggacttgcc gatcacgaga
      721 acatctcccg tctcgccgcc cttgatgcgg gccagcattg ttttggccga atccgcctcg
      781 atcgcgacct cctggccggc cgcccgctcg aactcgggtg caagcgcgtc gagcaccgag
      841 aacgagctgt gggaggtcag taccttgatc gccgaggggc cggcggcgcg gatctggggg
      901 ttcatcgtca ggacgggggg cgaaggatga attataggat gtcgaggggc aagggcataa
      961 tcctgaaggc acaaggcaca agcaagggca ctgccagact gaagttttct ttttcccttt
     1021 accttgtgac ttttgcctta acgaagggaa tgttccgttg cgcccggctg tttacgcatc
     1081 ggttttgttc cccagtcccc cacacaggag aagaccatgc accgagcctt tcttgcagcc
     1141 atcactgttg cagtgaccct ggcggccgca ccggccgggg cgcaggacaa gcctgcggcg
     1201 ccggccgcga agcccccatc gttctcgact accaggatcg agggcactga aaacgtctac
     1261 gcgttcacct ttggaaaccg ggtgtccatg ttcgtcgtca cgacggccgg cgtgatcgcc
     1321 accgaccccg gaggctacgg tcagccgcag cgcgtcccca cctatctcga cgagatcaga
     1381 aaggtcacgg accagccggt caagtacctg atctacagcc accaccacta cgaccacatt
     1441 gccggcggcc agccattcaa ggacgcaggc gcgcggatcg tcgcgcacaa gcgggccaag
     1501 gaacgcctca tgatgatcaa ggatccggca actccgctgc cggacgaggt cttcgacaag
     1561 aagcgggtga tcaagctcgg caacaccacg ctcgaactga cctaccacgg cctgaaccac
     1621 tcggacagca ccatcgccat gcgcttgccc agggagaaaa tcctgttcgt ggtggacatc
     1681 ctgccggtcg gctcggtgcc ggggcgcggc atgatcgatt tctacccgct cgaagccgag
     1741 aagtcgatcg aggagatact gaagctcgac tgggaccggc tgatcccggg ccacccgggg
     1801 cagcccggcg ggcggctcgg caccaaggcc gacgcgcagg cgcaactcgc cttcctgcgg
     1861 gatgcctcgg aaacggtgaa ggccgctgcg cgcgagggca agtgctggga ccgggtggag
     1921 aaggaactca agctgccgca atacgagtcg tggcccggct atcagaacaa cctgcaattc
     1981 gtgctgcggc gctactgcgg gctgtggggg cggggaacct gaatccagtg atgagtgatg
     2041 agtgatgaat gatgagtaaa accttcatcc cttgagcaaa caaaaaaccc cgccgaggcg
     2101 gggttttttg ttgtgaccaa cggccgacta gaagcggggg tttactttca tcattcctca
     2161 tccctccgcc ctcatccttg tgctccgagc gccgctcaga gcttgcgcag ctccgcgagc
     2221 agctccgggc tctcgggtat cgttccctgc gggtacactt tcgcatagcg gacgataccc
     2281 ttggcgtcga cgacgtaggc agcgcgcttg tccatgccgc gctcttcgtt gaagatgccg
     2341 taggccttgc cgaccgcgcc gtgcggccag taatccgaga gcagggggaa ggggacgccg
     2401 cccagctttt ccgaccagcc cttgaggctg ggtgcgggat cggcgctgat ttgcaggatc
     2461 tcgcagttca acttcttgaa ttcttcgtag ttcgcacgga acccgttgac ctggttcgtt
     2521 cacccgccgg tgaaagcgaa cggcatgaac gcgaccacga cgggccggcc cttgaagctc
     2581 gagagcgaga ttttctgatc ggtattgtgc gcgggcagcg cgaagtccgg tgctgctgcg
     2641 ccttccttga tatccattcg gctactcctg gctgtgagag ttggagacga agacaagttt
     2701 acagcatccg cgacgcggta gcccctgcta ccgctcttga ccgctccggc cgccggttta
     2761 ggccggggtc actgctcgcg attcacgctt catgtttcag gggattgcgc cttcatcctt
     2821 ttgaaggtca gccgggatag gcgtcgaacg cgatggcatc ggcctggcag cctaacgcct
     2881 tcgccatgct gttcagcgag tcgtcggtga gggcaaccac ctggtattcg gtctccaggc
     2941 ccttggcggc gaggaaggcc ctggccttgt cctgcgatgt ccacgttacg accatatccg
     3001 gcgtattgtt gccaacctgt cggctcaccg gaaagccgtc ggctttgcag atgattgcaa
     3061 acatgccgca actcctcctt gctatggtca gggtgacgcg atgaacagtg acgaagtgat
     3121 ggtttacgcg atttcatcac cccatcactg cgtcacctca tcgccggatc aacagtatcc
     3181 cgccgatgcg cctacagcgt cctgacctga accttgcgcc acttgatggc gccgccgggt
     3241 tccttgccgc ggttgccaaa ctggagcgag aacggtcccg aagcaaattt cttgtcctgc
     3301 aggctcacgg tgacggcgcc gttgaacttg acggtcactt ccgagccctt cgcgtatatt
     3361 tccagggtgt tccattttcc gccggccttg tggatgatcg gcaccggaac cattgcaaaa
     3421 ccgacgatgc ctcccgtagc gtactcctgg ccggggcgca tgtcgaagat gttcacctcg
     3481 taggcattgg cggcgcctat cttgttggga tcggacgcgc ggacgaagat gccgctgttg
     3541 gtggtagttt cggcccagaa ctcggcccgg atctcgaagt ccttgtagga gttcttcgtg
     3601 accaggtgcc cgcccttgcc cttgtcgccc acgatcgcgc cgccttccgc tcgccaattg
     3661 acgtcgccga tgcgattgaa attctccagg cccttctcgc cgtcgatgag cgtgacccac
     3721 cccgagcctg atgtggtcgc gcaaccgata acggtgagcg cggtaacaag cagccccatg
     3781 cagatgatag acaagcgttt cattgcaatc tcccccttgt ggatgacgat cgagtgaccg
     3841 ccacgctttt ggattctgct ccgctccgta atggtgtcaa tagccggggc ctgcggcagc
     3901 gggagcatat caagcggttg agtttattgt ccttttaatc agggcttgtc cccgtttatc
     3961 attgcggggt actgcttcaa ccaaccatcg aggggggact cagcatgaaa ctgccaataa
     4021 cgggcgcact gctgtgcgca tggagcgtgg tgtcgatgcc ggcggcgctg gcgcaaacgg
     4081 cgaaatatcc cgaccgcgcg atacggctga tcagcccctt tccgccaggc ggcaccgtgg
     4141 atgtgaacgc gcggattctc gccccggcgg tttcgaagtc cctggggcaa caggtcgtgg
     4201 tggacaaccg cgcgggggcg tccggcatca tcggcacggc ggaggcggca cgcgcgaagc
     4261 cggacggcta ctccctgctc atcaacacca cgccgctggt gacgaacacg ctgatgtaca
     4321 gcaaggtgcc gtacaacctg ctcaaggatt tcgagccgat ctcgatcctg tcgaccacgc
     4381 cttcgttcct gagcgtgcat ccgtcggtgc cggtcaagac cgtgcgcgag atgatccagc
     4441 tcgcgaaggc ccgccccggc gcgctcaatt acgccgccgc gggcgtcggc acgaatccgc
     4501 acattgcggg cgagctgctg aaccatctcg cgaagatcaa gctcctcgcc gtgcactaca
     4561 aaggcggcgg cccggcgacg gtcgcgacgt tgagcggcga gacgcagctt tacttcgtca
     4621 atgcgatccc ggaagcgctc ccgcacatac a
//
LOCUS       DBNGSG010000353         4190 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4020321, whole genome shotgun sequence.
ACCESSION   DBNGSG010000353 DBNGSG010000000
VERSION     DBNGSG010000353.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4020321"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="AC158Q_09160"
     CDS             <1..242
                     /locus_tag="AC158Q_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339985.1"
                     /translation="TVKSVAADDLALGAYSLTLPTGAPLRGQYGTGTLPIAFVVQAGA
                     AGKYAAEASAIGYQTQSVSADISVANATRDFILIP"
     gene            551..1687
                     /locus_tag="AC158Q_09165"
     CDS             551..1687
                     /locus_tag="AC158Q_09165"
                     /inference="COORDINATES: protein motif:HMM:NF023425.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ice-binding family protein"
                     /protein_id="HIR3339986.1"
                     /translation="MALLLAAFVTGCGGGGGGGAGTGNSVVPGAAGTPGASATNPTVG
                     SASPGDGATNVPTSTNSGSVVTGTLLSATFSEPMNPATIISPALTFTLTETTSGNNVP
                     GTVTMNGANTTATFTPAAALTPNTGYTATITTAARNAGGTPMPNPVSWSFTTSVVATT
                     GRAPVNLGAAGSFAVLTKTGITDVPNSAITGDVGASPITGAAILVTCAEVTGTIYSVD
                     AAGPLPCRVTNGTLLTTAIGDMEIAYTDAAGRTLPDFTELQAGNIGGLTLVPGLYKWS
                     SSVLIPTNVTLSGGPNDVWIFQIAGDLTQAAATSVILAGGAVPKNVFWQTFGAAAIGT
                     TAHFEGILMSQTSIVLGTGASVNGRLLAQTAVSLDQNAVTQPAP"
     gene            1817..2476
                     /locus_tag="AC158Q_09170"
     CDS             1817..2476
                     /locus_tag="AC158Q_09170"
                     /inference="COORDINATES: protein motif:HMM:NF017269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HIR3339987.1"
                     /translation="METQSNKSPHTLYWIAGIAVTVFSAVGIAAILGWIPTSVGSSSE
                     SVVVPMQPSAPAKPAAVAQAAPAQTRAQAREEAREAAESRARREARAQARTEMADRAT
                     ARASTRCAECGVVESVREIATKGEGSGIGAVGGAVVGGVLGHQVGGGSGQKIATVVGA
                     VGGAVAGNEIEKRVKAGKSYEITVRFEDGSNSVIQEANTTTWRVGDRVKVVNGVIQSN
                     A"
     gene            2510..2659
                     /locus_tag="AC158Q_09175"
     CDS             2510..2659
                     /locus_tag="AC158Q_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HIR3339988.1"
                     /translation="MLYTIAVVLIILWLLGLVSSYTIGGLIHVLLVIALVVILLGFIG
                     GRRSL"
     gene            2696..2830
                     /locus_tag="AC158Q_09180"
     CDS             2696..2830
                     /locus_tag="AC158Q_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013390837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HIR3339989.1"
                     /translation="MFQKILAALMLLGMLGAISGCSTMQGFGQDMERGGERIQEKASR
                     "
     gene            2860..3258
                     /locus_tag="AC158Q_09185"
     CDS             2860..3258
                     /locus_tag="AC158Q_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3339990.1"
                     /translation="MGIIPKTAIPASDEAIDTIAGIELKRREAEKEAGLAASCRSALR
                     HLHALLDREKEALQLHGPDAGRPANVEAVAAEIERVKKLAAATVQNHPSRHADPAGLR
                     QMSRQKSWQNAQRNPARNKGRRTMGRAGGR"
     gene            3282..>4190
                     /locus_tag="AC158Q_09190"
     CDS             3282..>4190
                     /locus_tag="AC158Q_09190"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DesA family fatty acid desaturase"
                     /protein_id="HIR3339991.1"
                     /translation="MNLLYSGVFDLPWWGYAIAALALTHITIVAVTVFLHRHQTHRAL
                     DLHPAVSHFFRLWLWLTTGMVTREWVAIHRKHHAKVETPEDPHSPQVLGINRVLWGGV
                     LLYVRESRNRATIERYGYGTSDDWLERHVYSRYMKLGLTLTGFADVILFGIVPGALIF
                     LTQIAWIPFWAAGVINGIGHYWGYRHWSTPDASTNIVPWGILIGGEELHNNHHAFTTS
                     ARLSSKWYEFDIGWMYIRLLEALGLAKVKRVAPVPRFAAPRTIPDLQTLLAVITHRYD
                     VQAKYAKTLKRIGTGARDAHALQTMHS"
BASE COUNT          819 a         1276 c         1368 g          727 t
ORIGIN      
        1 ttacggtgaa gtccgtggcg gctgatgatc tggcgctcgg tgcctactcg ttgaccttgc
       61 ccaccggtgc gccgctgcgc ggccagtacg gcacaggcac tctgccgatc gcatttgttg
      121 tgcaagccgg tgcggcggga aaatatgccg ccgaggcttc cgcgatcggg tatcaaaccc
      181 agtcggtcag tgcggacata tccgtggcca atgcaacgcg ggactttatc ctgattccgt
      241 agagcaaacg gttcggtaat cataagggct cctgccggag cccttatttt tggaactcaa
      301 gggtacccat gccgggagtg gcagcgcagg gcaaggccgt cgcgagacgg cctttttctt
      361 tccatatatg tacgccagcg tacggagcgc agggcggcga aaggggatag tcgccacacg
      421 gcgttcggcg ccgtgcgcag aaatccgcgc accgcatgta gcagccgtat accaatgcct
      481 ttcggcatcc catgaccagg agaagcacat gaaccgatcc ggaatctctc gcaaaccaca
      541 aatgtggtgg atggcattgc tgctggctgc tttcgtgacc gggtgcggtg gcggtggcgg
      601 cggaggcgca ggcacgggca attccgttgt cccgggcgcc gccggcacgc cgggtgcctc
      661 ggccaccaat ccgacggtgg gttctgcgag ccccggagat ggcgccacga acgtgccgac
      721 cagcaccaat agcggcagcg tggtgaccgg cacgttattg agcgcaacct tcagtgaacc
      781 gatgaacccg gcaacgatca tctcgccggc gctcacgttc acgctcacgg aaacgacaag
      841 cggaaacaac gtgcccggca ctgtcaccat gaatggcgcg aatacgacgg cgacctttac
      901 gccggcggcc gcgctgaccc cgaataccgg ttataccgcc acgataacca ccgcagccag
      961 aaatgcgggg ggcacgccaa tgcccaatcc cgtttcctgg agctttacga ccagcgtggt
     1021 tgcgactacc ggccgggcgc cggtcaatct tggagcggcg ggctctttcg cggtcctgac
     1081 aaaaacaggc atcactgacg ttcccaactc ggccattacc ggcgatgtgg gcgcgagccc
     1141 gatcaccggt gccgccatcc ttgtgacgtg cgcggaagtg acggggacca tctattccgt
     1201 ggatgcggcc ggtccgcttc catgccgggt gaccaacggc acattgctga ccacggccat
     1261 aggcgacatg gagatcgcgt acaccgatgc cgcgggacgg accttgcccg attttacgga
     1321 actgcaagct ggaaatattg gcgggctgac cctcgttccc ggcctctaca aatggagcag
     1381 cagtgtcctg attccgacga atgtcacgct ctcgggtggc cccaatgacg tctggatctt
     1441 ccagatagcg ggagatctga cccaggctgc tgccacgagc gtgatcctgg cgggcggcgc
     1501 agtgcccaaa aacgtcttct ggcagacctt cggcgccgcg gcgattggaa cgaccgcgca
     1561 ttttgaaggg attctgatgt cccagacatc gatcgttctg ggaaccggcg catcggtcaa
     1621 cggcaggctg ctggcgcaga cggcggtcag tctcgatcag aacgcggtta cacaacccgc
     1681 cccgtaatcc ctcatcaggc gcggtacaag gcagaaaggc cgtcgcgaga cggcctttct
     1741 catttcactg agtgtgcgtt ggcgaacaga gtatcccggc cgcgaagggg acaattgcgg
     1801 aaaaccgagg gaaaccatgg aaacgcaatc gaacaaatcc ccgcacacgc tgtattggat
     1861 cgcgggcatc gcggtgaccg tgttcagcgc cgtcggcatc gcggccatcc tgggctggat
     1921 cccgacctca gtcggcagtt ccagcgagag cgttgttgtt cccatgcagc cttcggcgcc
     1981 ggcgaaaccc gccgcggtag cgcaggccgc cccggcacag actcgagcgc aggcccggga
     2041 ggaagcccgg gaagccgccg agtcgcgggc ccgaagagaa gcccgggcgc aggcccgcac
     2101 ggagatggcg gacagagcca cggcccgcgc cagcacccgg tgtgccgaat gcggcgtggt
     2161 cgaatcggtg cgcgagattg ccacgaaagg cgaggggtcc ggcatcggcg cggtcggagg
     2221 agcggtcgtg ggcggcgtgc tcggccacca ggtcggcggc ggcagcgggc agaagatcgc
     2281 cacggtcgtc ggcgcggtgg gcggagcggt ggccggcaat gaaatcgaga aacgggtgaa
     2341 agcgggaaag agctacgaga ttaccgtccg cttcgaggac gggtcgaaca gcgtgataca
     2401 ggaagcgaac accacgacat ggcgcgtcgg cgaccgggtc aaggtcgtga acggcgtgat
     2461 ccagtcgaac gcctgaactt cgaatcactt aaaccaaagg ataattgcca tgctctatac
     2521 catcgctgtc gttctgatca tcttgtggct cctggggttg gtttcctcct acaccatcgg
     2581 cggcctcatt cacgttctgc tggtgatcgc cctcgtggtc atcctgctcg ggttcattgg
     2641 cgggcgaaga tccctgtaat ttcccgagct ttcatttcat tgacggagag acatcatgtt
     2701 tcaaaaaata ttggcggcat tgatgctgtt gggtatgttg ggcgcgattt cgggctgtag
     2761 caccatgcag ggcttcgggc aggacatgga gcggggcgga gaaaggatcc aggaaaaggc
     2821 gtcacgctga gcggaaacgt aatgccaggg gaggactcga tgggaatcat ccccaagacg
     2881 gctatcccgg caagcgatga ggcgatcgac acgattgccg gcatcgaatt gaagcggcgg
     2941 gaagcggaga aggaggccgg gcttgcggcg tcatgccgct cggctctgcg ccatttgcac
     3001 gcgcttctcg accgcgagaa ggaagccttg cagttgcacg gcccggacgc cggccgtccg
     3061 gcgaacgtcg aagccgttgc cgccgaaatc gagcgggtca agaaactggc cgctgccacg
     3121 gttcagaacc atccctccag gcacgcggat cccgccgggc tgcgccagat gtcgcggcag
     3181 aagtcgtggc agaacgcgca acggaatccg gccaggaaca agggccgcag gacgatggga
     3241 cgggccggtg ggcgctgacc gagtgtcgag tgacgggtga aatgaatctg ctttattccg
     3301 gtgtattcga tcttccgtgg tggggctacg cgatcgcggc cctggcgctg acccatatca
     3361 ccatcgtcgc ggttacggtg ttccttcacc gccaccagac gcaccgcgcg ctcgacctgc
     3421 atccggccgt gagccatttc ttccggctgt ggctgtggct gacgaccgga atggtgacga
     3481 gggagtgggt cgcgattcac cgcaagcacc acgccaaggt cgaaacgccg gaggatccgc
     3541 acagcccgca ggtgctgggc atcaaccggg tgctgtgggg cggggtcctg ttgtacgtca
     3601 gggagtcccg caaccgcgcg acgatcgaac gttacggtta cggcacctcc gatgactggc
     3661 tcgagcgcca tgtctactcc cgctacatga aactgggcct gacgctgacc ggcttcgccg
     3721 acgtcatcct gttcggcatc gtgcccgggg cgctgatatt cctgacgcag atcgcgtgga
     3781 ttccgttctg ggcggcgggc gtgatcaacg gcatcggcca ctactggggt tatcgccact
     3841 ggtccacgcc cgatgccagc accaacatcg tgccgtgggg catcctcatc ggcggcgagg
     3901 agctgcacaa caaccatcac gccttcacga cgtcggccag gctgtcgagc aagtggtacg
     3961 agttcgacat cggctggatg tacatccgcc tgctcgaggc gctggggctc gcgaaggtca
     4021 agcgcgttgc gccggtcccg cgattcgccg cgccccggac aatccccgac ctgcagacgc
     4081 tgctggcggt gattacccat cgctacgatg tccaggcaaa gtacgccaaa acgctgaagc
     4141 ggatcggaac cggcgcgcgg gacgcgcatg cgcttcagac catgcattcc
//
LOCUS       DBNGSG010000354         3328 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3172366, whole genome shotgun sequence.
ACCESSION   DBNGSG010000354 DBNGSG010000000
VERSION     DBNGSG010000354.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3172366"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1216
                     /gene="aroA"
                     /locus_tag="AC158Q_09195"
     CDS             <1..1216
                     /gene="aroA"
                     /locus_tag="AC158Q_09195"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HIR3339992.1"
                     /translation="AAGDTLIHDLLESDDTRVMLEALRKLGVDWASAGARAMRVRGTA
                     GAFPVKRAQLSLGNAGTVFRPLTAVLALMGGDYRLEGVARMHERPIGDLVDALNSLGA
                     RIEYLGNPGYPPLAISPAAIRAGAAVSVRGNVSSQYLTGLLVALPLTGTRTVISVEGD
                     LVSKPYVEITLNMMRKFGVEVVREDWTRFTIPAAHYASPGEIYVEGDASSASYFLAAG
                     AISGLSGGGPVRVEGVGRASIQGDVRFAEILERMGARITMGENWIEAGAFHPARPGGK
                     LKALDADLNHIPDAAMTAAIAALFADGPSMLRNIGSWRVKETDRIKAMAAELRKLGAT
                     VEEGPDYLRVTPPPALQPATIDTYDDHRMAMCFSLAALGGVRLRINDPACVAKTFPEF
                     FDVLASITEPRA"
     gene            1213..1884
                     /gene="cmk"
                     /locus_tag="AC158Q_09200"
     CDS             1213..1884
                     /gene="cmk"
                     /locus_tag="AC158Q_09200"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0036431 - dCMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HIR3339993.1"
                     /translation="MSTLPPVIAIDGPSASGKGTVAQGVAGALGFHYLNSGALYRTVA
                     VAALKAGGNLESESELSSIALNIKAEFYGDSVQLEGRDVTDAIRTEEISAAASRIAAY
                     PRVRSALLERQKGFRKPPGLVADGRDMGTVVFPDAVLKIYLTASPEQRAARRYKQLMG
                     KGLGATMAALLQDIRERDQRDSARAAAPLKKGSDAVLIDTTNRSAADAIAQVLALYKQ
                     APKAL"
     gene            1956..>3328
                     /gene="rpsA"
                     /locus_tag="AC158Q_09205"
     CDS             1956..>3328
                     /gene="rpsA"
                     /locus_tag="AC158Q_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HIR3339994.1"
                     /translation="MMKAPTQAAPMESFAALFEESLSRKEMRVGEVITAEVIRVDDNY
                     VVVNAGLKSESYIPLEEFRNDRGETEAKPGDFVKVAIEALEDGHGETRLSRDKAKRLA
                     AWLDLEEALEKGNVVSGVINGKVKGGLTVTVNGIRAFLPGSLVDIRPVKDTTPYENKA
                     MDFKVIKLDRKRNNVVVSRRAVLEASQGEERQKLLENLQEGKVVKGIVKNITDYGAFV
                     DLGGIDGLLHITDLAWRRVKHPSEILTVGDEVEAKVLKFDQEKNRVSLGMKQLGEDPW
                     VGLSRRYPPGTRLFGKVSNLTDYGAFVEIEAGIEGLVHVSEMDWTNKNVHPSKVAQLG
                     DEVEVMVLEIDEERRRISLGMKQCMPNPWEEFAMNHKKGDKVRGQIKSITDFGIFIGL
                     PGGIDGLVHLSDLSWSKPGEEAVRKYTKGQEVEAVVLAIDGSRERISLGVKQMASDPL
                     TQYMAEHP"
BASE COUNT          651 a         1005 c         1120 g          552 t
ORIGIN      
        1 ggctgcgggc gacaccctca tccacgacct gctcgagtcc gacgacaccc gggtcatgct
       61 cgaggcgttg cgcaagctgg gcgtcgactg ggcaagcgcg ggcgcgcggg cgatgcgggt
      121 tcgtggcacg gccggcgcgt ttccggtcaa gcgcgcgcaa ctctcgctcg gcaacgccgg
      181 cacggtgttc aggccgctta ccgcggtgct ggcgctgatg ggcggcgact accggctgga
      241 aggggtggcg cgcatgcacg agcggccgat cggcgacctg gtggatgcct tgaatagcct
      301 tggcgcccgc atcgaatacc tcggcaaccc cggatacccg ccgctggcca tttcccccgc
      361 cgcaattcgc gcgggcgccg cagtcagcgt gcgtggcaac gtatcgagcc agtacctgac
      421 gggtttgctg gtcgcgctgc cgctcaccgg caccaggacg gttatcagtg tcgagggcga
      481 cctggtgtcg aagccgtacg tcgagattac gctgaacatg atgcgaaagt ttggcgtcga
      541 ggtggtgcgc gaggactgga cgaggttcac gataccggcc gcgcattacg cgagtccggg
      601 ggagatctac gtcgagggcg acgcgtcttc ggcctcgtac tttctcgcgg cgggtgcgat
      661 cagcggactc tccggcggcg ggccggtgcg cgtcgaaggc gtggggcgcg cgagcataca
      721 aggcgacgtg cgctttgccg aaatcctcga gcgcatgggc gcgagaatca cgatggggga
      781 gaactggatc gaggcgggcg ccttccatcc ggcgcggccc ggcggcaagt tgaaggcact
      841 ggacgccgat ctgaatcaca ttcccgatgc ggccatgacg gcggcaatcg cggccctgtt
      901 tgccgatggt cccagcatgc tgcgcaatat cggcagctgg cgcgtgaagg agaccgatcg
      961 gatcaaagcc atggcggccg aattgcgcaa gctgggcgcc acggtggagg agggcccgga
     1021 ctatctgagg gttactccgc cgccggcgct gcaaccggcg accatcgaca cctacgacga
     1081 ccaccgcatg gcgatgtgtt tttcgctcgc ggcgctgggc ggggtcaggc tgcggatcaa
     1141 cgatccggct tgcgtcgcca agacattccc cgaattcttc gatgtgctcg cctcgattac
     1201 ggagccccgg gcttgagcac gttgccccct gtcatcgcca tagacggccc gtcggcgtct
     1261 gggaagggca cggtggcgca aggtgtggcc ggtgcgctcg ggtttcatta cctgaatagc
     1321 ggtgcccttt accggaccgt ggcagtggcc gcgctgaagg ccgggggaaa cctagaaagt
     1381 gaatcagaat tatccagtat tgcactgaat ataaaggctg aattttatgg agatagcgtc
     1441 caacttgagg gacgggatgt gaccgatgcg attcgaacgg aagagattag cgctgcggca
     1501 tcccgtattg cggcctatcc gagggttcga agcgccctgc tcgaacggca aaagggattc
     1561 cgtaaacccc ccggactggt ggcggacggc cgcgacatgg gcacggtcgt gtttcccgat
     1621 gccgtgctca aaatctacct caccgcgagc ccggagcaac gggcggcacg gcggtataaa
     1681 cagttgatgg gaaaaggatt gggtgctact atggcggccc ttttgcagga tatccgggag
     1741 cgcgatcaac gagacagtgc gcgcgccgcg gctcccctga aaaagggctc ggacgccgtt
     1801 ctgatcgata ccacgaaccg atcggcagcc gatgcgattg cgcaggtgct ggcgctctac
     1861 aaacaagcac caaaggcgct gtgagccgca ccccggttca tggcttgatc aaccaacccc
     1921 tgtcgtcaag gcgggcatcc aacatcctga actgaatgat gaaagcaccc acccaggcag
     1981 cgcccatgga aagcttcgcc gcgctgtttg aagaaagtct ctcccgcaag gaaatgcggg
     2041 tgggcgaagt gatcaccgcc gaggtgatcc gtgtcgatga taactacgtc gtcgtcaatg
     2101 ccggcctgaa atccgagagt tacatcccgc tggaggaatt ccgcaacgac cgcggcgaaa
     2161 ccgaggccaa gcccggcgac ttcgtcaagg tcgcgatcga ggccctcgaa gacggccatg
     2221 gcgaaacacg gctctcgcgc gacaaggcga agcggctggc ggcctggctc gacctggaag
     2281 aagcgctgga gaagggcaac gtggtgtccg gcgtcatcaa cggcaaggtc aagggcgggc
     2341 tcaccgtcac ggtgaacggc atccgcgcct tccttcccgg ctcgctggtg gatatccgcc
     2401 cggtcaagga caccacgccg tacgagaaca aggcgatgga tttcaaggtc atcaagctcg
     2461 atcgcaaacg caacaacgtg gtggtttcgc gccgcgccgt gctcgaggcc tcgcagggcg
     2521 aggagcgcca gaagttgctg gagaatctgc aggaaggcaa ggtggtgaag ggcatcgtca
     2581 agaacattac cgactacggc gcgttcgtgg acctgggcgg catcgacggc ctgctccaca
     2641 tcaccgatct cgcgtggcgc cgcgtgaagc atccgtcgga aatcctcacc gtgggcgacg
     2701 aggtcgaggc caaggtgctg aaattcgacc aggagaagaa ccgggtttcg ctcggcatga
     2761 agcaactcgg cgaggatccc tgggtggggc tgtcgcgccg ctatccgccc ggcacgcggt
     2821 tgttcggcaa ggtctccaac ctcaccgatt acggcgcgtt cgtcgagatc gaggccggca
     2881 tcgagggcct ggtgcacgtc tccgagatgg actggaccaa caagaacgtc catccatcga
     2941 aggtggcgca gctcggcgac gaggtcgagg tcatggtgct cgagatcgac gaggagcgcc
     3001 gccgcatctc gctgggcatg aagcagtgca tgcccaaccc gtgggaagag ttcgccatga
     3061 accacaagaa gggcgacaag gtccgcgggc agatcaagtc catcaccgat ttcggcatct
     3121 ttatcgggct gcccggcggc atcgacggcc tggtgcacct ctccgacctt tcctggagca
     3181 agcccggcga ggaagccgtg cgcaagtaca ccaagggcca ggaagtcgag gccgtggtgc
     3241 tcgccatcga cggctcgcgc gagcgcatct cgctgggcgt caagcagatg gcctccgatc
     3301 cgctcaccca gtacatggcg gagcaccc
//
LOCUS       DBNGSG010000355         2532 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1813406, whole genome shotgun sequence.
ACCESSION   DBNGSG010000355 DBNGSG010000000
VERSION     DBNGSG010000355.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1813406"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="AC158Q_09210"
     CDS             <1..645
                     /locus_tag="AC158Q_09210"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3339995.1"
                     /translation="TLRDFIPVTLMGTSAQFLTANAAVPANTLAELIKLIKSKPGQMR
                     YGSGGIGSVHHLSMEALKTPLGLDITHVPYKGNGVAIPAMIGGEVALVFSALPAIEAH
                     LKTGRVKLLAVNTQKRSAQAPNVPTVAEVTGIKDYDYPPAIGVLAPAKTPKAVVNRLA
                     DAFARAVQHPETSKRYITLGIEPVGNTPEQYAKQVRIDIAKYAKAVKASGVKVE"
     gene            865..1746
                     /locus_tag="AC158Q_09215"
     CDS             865..1746
                     /locus_tag="AC158Q_09215"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3339996.1"
                     /translation="MRIIAPFAPGGTADTLGRIVAAKLTESLGQSFVVENRPGAGGLV
                     GSDLVAKSAPDGYTLGVSGVASHAIAPALAKKPPFDPLKDFTHVALFGGPPAVLGVHP
                     SLPVKDLKGFVAFAKARPGELTYGSPGNGTQGHLVAEVFKQATGINIIHVPYKGAAIA
                     VVDIVAGHIHSISTTLTTASVQIRAGRIRPLAISSAKRLPDYPGVPTFQEAGYRNLVA
                     TVWFSLSGPPGMPADIVNKLNGEVRRILQLPDVRERLSGDGIEPNQLDAKAFGDYMAA
                     EVKRWAPVVRASGARAN"
     gene            1885..2244
                     /locus_tag="AC158Q_09220"
     CDS             1885..2244
                     /locus_tag="AC158Q_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018423368.1"
                     /note="extradiol catechol dioxygenase that catalyzes the
                     oxidative cleavage of substituted catechols; part of the
                     bacterial aromatic compound degradation pathway; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase"
                     /protein_id="HIR3339997.1"
                     /translation="MNPQLEGVESLEGTYLFTLERSSKAMRLNRFLHGLTVPANRALF
                     RDDAPAAFDKAGLDAEERKLVSDRDWAGLIRYGASFFCLEKLARVSGVSNPEMVAAMR
                     GETLEEFLKTRNVPGAR"
     gene            complement(2351..>2532)
                     /locus_tag="AC158Q_09225"
     CDS             complement(2351..>2532)
                     /locus_tag="AC158Q_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3339998.1"
                     /translation="LDRLRAETGRLLADPGALGRIRGIALDPYVTTPQEFTALIRADH
                     TRYGEVVRAVGVKVD"
BASE COUNT          501 a          811 c          803 g          417 t
ORIGIN      
        1 acgctccggg acttcattcc ggtcacgctg atgggtacct cggcgcagtt cctgacggcg
       61 aacgccgccg tgccggcgaa cacgctcgcg gaactcatca agctcatcaa gagcaagccg
      121 ggccagatgc gctacggctc gggcggcatc ggcagcgtcc accatctcag catggaagcg
      181 ctcaagacgc cgctcggcct cgacatcacc cacgtgccgt acaagggcaa cggggtggcc
      241 atccccgcga tgatcggagg tgaagtggcg ctcgtcttct ccgccctgcc cgcgatcgag
      301 gcgcacctca agacgggacg ggtaaagctg ctcgcggtga atacccagaa gcgcagcgcg
      361 caggcgccga acgtgccgac cgtcgccgaa gtgacgggaa tcaaggacta cgactacccg
      421 cccgcgatcg gcgtgctcgc gccggcgaaa acgccgaagg ccgtcgtcaa ccggctcgcg
      481 gacgcgtttg ccagggccgt tcaacacccg gagacgtcca aacgctacat cacgctgggc
      541 atcgagcccg tgggcaacac gccggagcag tacgcgaaac aggttcgcat cgacatcgcg
      601 aagtacgcga aagcggtcaa ggcatccggc gtgaaagttg aataagggaa aagaggaaag
      661 ggaaatgagg aaaggggaaa gggaaaagga aaaagggaaa agtgtgagaa tcgctttacg
      721 aaccttgcgc gctattgagg aaagacaatg attggcacga gaagacccct ggggtttggt
      781 ctgtacctat ccttttccct tttccctttt gccttttgcc ttgggatagc ttgcgcgaac
      841 gcgcagggct atcccgcgaa gacggtgagg atcatcgcgc cgttcgcgcc gggcggcacc
      901 gcggatacgc tcgggcgcat cgtcgccgcc aagctcaccg agagcctggg ccagagcttc
      961 gtggtggaga accggcccgg cgccggcggc ctggttgggt cggacctggt cgcgaagagc
     1021 gcccccgacg gctacacgct cggcgtttcc ggcgtcgcgt cgcacgcgat cgcgccggcc
     1081 ctggcgaaga agcccccctt cgacccgctc aaggatttca cccatgtcgc gctcttcggc
     1141 ggcccgcccg ccgtgctggg cgtgcatccg tcgctgccgg tgaaggacct caagggattc
     1201 gtcgcgttcg ccaaggcaag gcccggggaa ctgacctacg gctcgcccgg caacggcacc
     1261 cagggccacc tcgtcgccga ggtcttcaag caggccaccg gcatcaacat catccacgtg
     1321 ccctacaagg gcgccgccat cgccgtggtg gacatcgttg cgggccatat acactcgatt
     1381 tcgacaacct tgacgaccgc cagcgtgcag atccgcgccg gccgcatccg gccgctcgcg
     1441 atcagttcgg ccaaacggct ccccgactac cccggtgtcc ccaccttcca ggaagccggc
     1501 taccgcaacc tggtcgccac ggtctggttc tcgttgtccg gcccgccggg gatgccggcc
     1561 gacatcgtga acaagctgaa cggcgaggtg cggcggatac tgcagcttcc cgatgtgcgc
     1621 gagcggctga gcggcgacgg catcgagccg aaccagctcg acgcgaaagc gttcggcgac
     1681 tacatggccg ccgaggtgaa acgctgggcg ccggtcgtgc gcgcctcggg cgcccgcgcc
     1741 aactaaaaag cgttaataga caggatttac aagatttaca ggattagatc gaaaagcaag
     1801 accgggccgc ataatccaat ttgggtttgg tttgatcttg ttaatcctgt ctattttttt
     1861 gatcttgtct tctggaaaac acgcgtgaat ccgcagctgg aaggcgtcga gtcgctggaa
     1921 gggacctacc tgttcacgct cgagcgcagc agcaaggcga tgcgcctgaa ccgcttcctg
     1981 cacgggttga ccgttccggc caaccgcgcc ctcttccgcg acgacgcgcc agcggccttc
     2041 gacaaggccg gacttgatgc ggaagaaagg aaactcgtca gcgaccgcga ctgggccggc
     2101 ctgatccgct acggggcgag cttcttctgt ctcgagaagc tcgcgcgcgt ctccggcgtc
     2161 tccaatccgg aaatggtcgc ggcgatgcgc ggcgagacgc tcgaggaatt tctcaagacc
     2221 cggaatgtgc ccggggcgag atgactcgcc ccctcaagga tgagggcgga gagatgagga
     2281 attggccaaa tacccgatgc gagcctctcc tcatccctca taaatcatcc ctcatccctt
     2341 aaaccgtcgg ctagtcaacc ttgacgccga cggctctcac cacctcgcca tagcgggtgt
     2401 gatcagcgcg gatcagcgcg gtgaattcct gcggcgtagt gacgtagggg tcgagtgcga
     2461 tgccgcggat gcgtccgagg gcgccgggat cggcgaggag ccggccggtt tcggcgcgca
     2521 gccggtcaag ca
//
LOCUS       DBNGSG010000356         2224 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1813422, whole genome shotgun sequence.
ACCESSION   DBNGSG010000356 DBNGSG010000000
VERSION     DBNGSG010000356.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1813422"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="AC158Q_09230"
     CDS             complement(<1..500)
                     /locus_tag="AC158Q_09230"
                     /inference="COORDINATES: protein motif:HMM:NF013308.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HIR3339999.1"
                     /translation="MPKYPKSYALIPAAGTGARMGAARPKQYVEIAGRPLLYYALRSL
                     ARHPVIEQVFVVLAQGDDRFNRCDWREFGTKIKPLYCGGETRSVSVFNGLLAARDTIG
                     GADWVLVHDAARPCLGREELDRLFGELEGEDTGGLLAVPLADTLKRSNRESRIARTEP
                     RDNLWLA"
     gene            671..1498
                     /gene="amrB"
                     /locus_tag="AC158Q_09235"
     CDS             671..1498
                     /gene="amrB"
                     /locus_tag="AC158Q_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HIR3340000.1"
                     /translation="MSQVPSTSVRAPAVAGMFYPGESRELAHSLAQLLGAAAHDAPER
                     AVPKAIIAPHAGYIYSGPVAASVYAQLAPARSRITRVVLLGPTHRVAVRGLALPGCSA
                     FATPLGTVEIDQAAVERLAGMSQVTVSPQAHALEHSLEVHVPFLQAVLEKFTLVPLAV
                     GHAGAQEVADVLDALWGGSETLIVVSSDLSHYLSYGDAQAVDRATAKAIVGLATDLSH
                     EQACGATPVTGLTHLARRRGLKPELVDLRNSGDTAGDRNRVVGYGAFAFYEVARDAR"
     gene            1488..2075
                     /gene="amrA"
                     /locus_tag="AC158Q_09240"
     CDS             1488..2075
                     /gene="amrA"
                     /locus_tag="AC158Q_09240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HIR3340001.1"
                     /translation="MPADSGRDGPHPFPPDAGHVLLPLARTAIATELRLVHPASAEQP
                     WLRQEGASFITLMREGRLRGCIGTLRPHRTLADDVKANAIGAAFRDPRFTPLTVEEFA
                     AVSVEVSVLSALEPMTFGDEADALKQLRAGTDGVVFQYGHHSSTFLPQVWEQFGEPAE
                     FLAHLKYKAGLPPDFWDKDVRLSRYTVFKWRETDS"
BASE COUNT          387 a          775 c          702 g          360 t
ORIGIN      
        1 gcgagccaca ggttgtcgcg cggctcggtg cgcgcgatgc gcgactcgcg attggagcgt
       61 ttcagcgtgt ccgccagcgg caccgccagc agcccgccgg tgtcttcccc ctcgagctcc
      121 ccgaacagcc ggtcaagctc ctcgcgcccg aggcagggcc gcgccgcatc gtgcaccagc
      181 acccagtcag ccccgccgat cgtgtcgcgc gccgcgagca gcccgttgaa cacgctcacc
      241 gaacgcgtct cgccgccgca atagagcggc ttgatcttcg ttccgaattc gcgccagtcg
      301 cagcgattga aacgatcgtc gccctgcgca agcacgacga agacctgctc gatcaccgga
      361 tgccgcgcca gcgaccgcag cgcgtagtag agcagcggcc ggcccgcgat ttccacgtac
      421 tgcttcggcc gcgccgcgcc catgcgcgcg ccggtgccgg cggcgggaat cagggcgtaa
      481 gacttcgggt atttcggcac gctgacaaga cctctgtaat tgcagggatt ttatcagcaa
      541 caggtgttgc cacttccgcc cttgaaaatg ccccaaccgg gcgcataatt cagcagtgga
      601 aatgagatcc tttttgcgct ccggtcgccg atccaccctc acgctgacgc gcgggtgata
      661 ggcgctgtca atgagccagg tcccttcaac atccgtgcgc gcgccggcgg ttgccggcat
      721 gttctacccg ggcgagagtc gagagctcgc gcacagcctg gcgcaattgc tgggcgccgc
      781 cgcgcacgat gcgcccgagc gcgccgtgcc caaggccatc atcgcgcccc acgccggcta
      841 catctattcc ggcccggttg cagccagcgt ttacgcgcag ctcgctcccg cgcgcagccg
      901 catcacccgc gtcgtcctgc tcggccccac gcaccgcgtc gcggtccggg gcctggcgct
      961 gccggggtgc agcgcgtttg ccacgccgct cggaacggtc gagatcgatc aggccgcggt
     1021 cgaacggctc gccggaatgt cccaggtgac agtgagcccg caagcgcacg ccctcgagca
     1081 ttcgctggaa gtacatgtgc cgttcctgca ggcggtgctc gagaaattca cgctggtgcc
     1141 gctggcagtc ggccacgcgg gcgcgcagga ggtcgcggac gtgctcgatg cgctgtgggg
     1201 cggatccgag acgctcatcg tggtgagttc cgacctctcg cactatctct cctacggtga
     1261 tgcgcaggcc gtggaccgcg cgaccgccaa ggccatcgtc gggctcgcaa ccgatctctc
     1321 gcacgagcag gcgtgcggcg cgacgccagt gacgggactc acccacctcg cgcgccggcg
     1381 cgggttgaag cccgaactgg tcgatctccg caattccggc gataccgcgg gagacaggaa
     1441 ccgcgtggtc ggctacggcg ctttcgcgtt ctacgaggtc gcgcgggatg cccgctgatt
     1501 ccgggcggga cggcccgcat ccattcccgc ccgacgcggg ccacgtgctg ctgccgctgg
     1561 cgcgcacggc tatcgcgacc gagttgaggc tcgttcatcc cgcgagcgcc gagcaaccgt
     1621 ggctcaggca ggagggcgcc tccttcatca ccctgatgcg ggaaggcaga ttgcgcggtt
     1681 gtatcggcac cctgcgcccg caccgtacgc tggccgacga tgtgaaagcc aacgcgatcg
     1741 gcgccgcgtt ccgcgatccc cgcttcacgc cgctcaccgt ggaagagttt gccgccgtat
     1801 cagtcgaggt ctcggtgctg tccgcgctcg aacccatgac cttcggcgac gaggcggacg
     1861 cgctgaagca gttgcgcgcc ggcacggacg gcgtcgtctt ccagtacggg caccactcca
     1921 gcaccttcct gccccaggtc tgggagcaat tcggcgaacc ggccgaattc ctcgcccacc
     1981 tcaagtacaa ggcgggcctg ccgccggatt tctgggacaa ggacgtccgc ctctcgcgct
     2041 acaccgtctt caaatggcgt gaaaccgatt cgtgaaaacc ggtaatggtg agaaccagaa
     2101 attagccgcc aagacgccaa gaacgccaag gaaaacgaga attaagatca agaccaagaa
     2161 aacatcaaaa aaacgctaag tggtgtaagc gctccacgtg tcaaatgcgt tatttcccgg
     2221 gagg
//
LOCUS       DBNGSG010000357         5994 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1475923, whole genome shotgun sequence.
ACCESSION   DBNGSG010000357 DBNGSG010000000
VERSION     DBNGSG010000357.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5994)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5994)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5994
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1475923"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="AC158Q_09245"
     CDS             complement(<1..214)
                     /locus_tag="AC158Q_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010090524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HIR3340002.1"
                     /translation="MPDAAAQLQNLLKRRILILDGAMGTMIQGYRLTEADYRGSRLRD
                     HPHDLKGNNDLLTLTRPQVIREIHEQY"
     gene            332..718
                     /locus_tag="AC158Q_09250"
     CDS             332..718
                     /locus_tag="AC158Q_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR3340003.1"
                     /translation="MKHFEPRQAHEFLKKNPNAIFIDCRSEMEYLFVGHPTGAILVPW
                     NDGPNWEVNPEFVAHVKKAASVDRPIILICRSGNRSLDAGRALEEAGFTQVWNVLHGF
                     EGELDENHHRNAKTGWRHEGLPWEQC"
     gene            complement(770..1981)
                     /locus_tag="AC158Q_09255"
     CDS             complement(770..1981)
                     /locus_tag="AC158Q_09255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HIR3340004.1"
                     /translation="MQKNSVSAPLLAAILGGLAMIGPFSVDTYLPSFPAIGQAFAATP
                     PQLQQTLSVYLITFAVMTLFHGTLSDSFGRRPVILGCLITYTLGSIGCAIAQSFGELL
                     LFRGVQGLAGGAGWVIGRAIVRDSFEGHDAQRMLSLVTMIFGIAPALAPVIGGWLQGA
                     FGWRAVFVFLIAYGGVMLAVCWFKLPETHPVSARQPFELKPLAANYLKLAGNPLMGLL
                     CLSAALNFAGFFLYVAAAPAFIYDLLGLGEKHFPALFVPMVAGIMVGASLSGRLAGRF
                     SPRRTMKTGFWILFGATGWNIGYHALFAPALPWSVAPVVCYAIGMALTAPSIQLLVLD
                     MFPENRGLASSLQGFTHGLLTSLTAGLIAPFLSGAALTLALGQLGLLALGCLCWLGYR
                     RRAGITPSVQC"
     gene            complement(2023..2832)
                     /locus_tag="AC158Q_09260"
     CDS             complement(2023..2832)
                     /locus_tag="AC158Q_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340005.1"
                     /translation="MGLSTRLRRLIDAAAPYWAGEAAVFEAYWTWEKRTRETDLAWLN
                     HQCFKEIWGSGVADKNLGLFLGPLEELKAAFPKIDIELDRHAVLDTAEALWAEFAHYC
                     AFADAHDGLLLPGEQKINPKRLKWWKEDEELANVRYGHIERHGRLGQRAARFTEGGYC
                     TLFSAGMKLNERPTPGHEHADALIATACGKVYDDEFGHMLKGVAGLDGEGLTDAEWNT
                     LAEISIEQLRNRIVMRNAQFGRPLSGARLADALAGRLQPVRFDYERARLAA"
     gene            2920..4404
                     /locus_tag="AC158Q_09265"
     CDS             2920..4404
                     /locus_tag="AC158Q_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3340006.1"
                     /translation="MNEIKEPVAAGGRIDAILPSHRDLYYDGQWQKPGGGYQDTLNPA
                     TDRSLGPCAEANAQDVDAAVRAAHKAFKSWRAVKPLERAALMKKVAAVLRANAEELAM
                     LDAANCGNPVREMVRDAGTAAAQWDFFAGLVTEMKGETIPMGDGVVNLSVREPFGVCA
                     RIVAYNHPLMFTAAKASPAIAAGNAVIMKPPYQAPLSAYRFVELIDGILPPGVLNILT
                     CGTAGSQALVAHPMVPRVSLIGSVPTGRAIMKAAADRLKHVSLELGGKNACIIYPDAD
                     LEKATRGAVGGMNFSWCGQSCGSTSRLFIHESVYDRVLEGVLKHVRHHKPGIPTEMAT
                     TMGAIVSRTQMEKVLSYIEIGKQEGATLVYGGKRPDDPRLAGGFFVEPTVFTNVTQKM
                     RIATEEIFGPVLSVLKWKDEETLFEQVNAVEHGLTGSVWTTSLANAHRAASRIESGFI
                     WVNGAGPHYLGVSFGGYKQSGIGREESYDELMSFTQSKNINITL"
     gene            complement(4517..>5994)
                     /locus_tag="AC158Q_09270"
     CDS             complement(4517..>5994)
                     /locus_tag="AC158Q_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340007.1"
                     /translation="ASGTNVGTYNDSLSNATGTGLGNYTISYVNGSLTITPAVISLAG
                     TRMYDGTISFGPAAFGSVSGVNGETLTVAGGAGSVASPLVGAYSLSAGTLALGNGTGL
                     ASNYTLTGGVHAATIVPDPANFRWSAGSGGNWDVASNWNQGVVPVAGAVVTIPDIGIP
                     GVSDTITYRSASGTTSIKSVTSAEALTVSGGMLNLGTTSADVSTIPLITLAGGTLGGA
                     GTVNVGMTNVLAGGVLSGTGTIVGNVNNSAGTVAPGASPGTLTIDGNYTQGPGGTLAV
                     EIGGAAAGTQYDQLIVTGNATLGGTLNVALINGFVPPSDSSFTVVQSGSTVSGVFSAT
                     NLPAAQPMTTSYLASMVNVTTALPTVPDQITNPQIDSSNDSPPNSEVVLGGPGTPTSA
                     GPNVTNIYLETTTGQIVPLIPASTIEPGSYVNLDTGQTVILTSGTTLEPGIYLDPVTQ
                     TVFVVTIDEVTGQLVIMTASVQGAQIASDKGVQVGKMGACR"
BASE COUNT         1135 a         1969 c         1915 g          975 t
ORIGIN      
        1 gatactgctc gtggatctcg cggatgacct gcggccgcgt gagcgtgagc agatcgttgt
       61 tgcccttcag gtcgtgcgga tggtcccgca accggctgcc gcggtaatcc gcctccgtca
      121 gccgataacc ctggatcatg gtgcccatgg caccgtcgag aatcaggatg cggcgcttga
      181 gcaggttctg gagttgtgcg gcggcgtcag gcatggatcg ggaaagacag aaagtccctg
      241 atgcaaaagg cggaattcta caggcaaact ggcggacggc gcagggttgc gactataatt
      301 ccgctcccgg tagcgatagc ggaaattttc gatgaagcat ttcgagccca ggcaggccca
      361 cgagttcctg aagaagaacc cgaacgcgat cttcatcgac tgccgcagcg agatggagta
      421 cctgttcgtc ggccacccga ccggcgcgat tctcgtgcca tggaacgacg ggccgaactg
      481 ggaagtgaat cccgaattcg tggcgcacgt gaagaaggcc gcgagcgtgg accgtcccat
      541 catcctcatc tgccgcagcg gcaaccgctc gctcgacgcc ggccgcgcgc tggaggaggc
      601 cggcttcacc caggtctgga acgtgctgca cggtttcgaa ggcgagctcg acgagaacca
      661 ccaccgcaat gccaagaccg ggtggcgcca cgaagggctg ccctgggagc agtgctgagt
      721 gttgagtgct tagtgttgag ttgaatgcaa accattcaac actcaaaact caacactgaa
      781 cactcggcgt gatgccggcc cggcgccggt aacccagcca gcaaaggcat cccagggcga
      841 gcagcccgag ctggccgagc gcgagcgtaa gcgcggctcc cgagagaaag ggcgctatca
      901 gcccggcggt gagcgaggtc agcaggccgt gcgtgaaccc ctgcagggat gacgcgaggc
      961 cgcgattctc cggaaacatg tcgagcacca gcagctggat gctcggcgcg gttagcgcca
     1021 tgccgatcgc ataacacacg accggagcca ccgaccacgg cagcgccggc gcgaacagcg
     1081 cgtggtaacc gatgttccag cccgtcgcgc cgaacagtat ccaaaagccc gtcttcatcg
     1141 tccggcgcgg cgaaaaccgg cccgccagcc gccccgacag ggaagcgccc accatgatgc
     1201 cggcgaccat cggcacgaac agcgccggaa aatgcttctc ccccaggccg agcaggtcat
     1261 agatgaaagc cggcgccgcg gcaacgtaca gaaagaaccc ggcgaaattg agcgcggcag
     1321 acaggcacag caatcccatc agcggattgc cggcgagctt gagatagttg gccgcaagcg
     1381 gcttcagctc gaagggctgg cgcgccgata ccgggtgcgt ctcgggcagc ttgaaccagc
     1441 acaccgcgag catcaccccg ccgtatgcga tgaggaacac gaataccgcg cgccagccga
     1501 acgcgccctg cagccaaccg ccgatcaccg gcgccagtgc cggcgcgatc ccgaagatca
     1561 tggtcaccag cgacagcatc cgctgggcat catgtccctc gaagctgtcg cggacgatcg
     1621 cgcggccgat gacccatccc gcgccccccg ccaaaccctg cacgccccgg aacagcagca
     1681 gctccccgaa actctgcgcg atcgcgcaac cgatcgaacc cagcgtatag gtgatgaggc
     1741 agcccaggat cacgggacgc cgcccgaacg agtcggacag cgtgccgtgg aacagcgtca
     1801 tcacggcgaa tgtgatgaga tagaccgaga gcgtctgctg cagttggggc ggcgtagccg
     1861 cgaacgcctg gccgatggcc ggaaacgacg gcaggtaggt gtcgaccgag aacggcccga
     1921 tcatcgccag cccgccgagt atggcggcaa gcaatggcgc cgaaacgctg ttcttctgca
     1981 aggagagggt gccgggcggc tacgcccggc gcgccggttc gttcacgccg cgagccgcgc
     2041 ccgctcgtaa tcgaagcgaa ccggctgcag gcgccccgcc agcgcgtcgg caagccgggc
     2101 tccgcttaac gggcggccga actgggcatt gcgcatcacg atgcggttac gcagctgctc
     2161 gatcgagatt tccgcgagcg tattccactc tgcgtccgtc aacccctcgc cgtccagccc
     2221 ggcaacgccc ttcagcatgt ggccgaattc gtcgtcgtag accttgccgc aggccgtggc
     2281 gatgagggcg tcggcgtgct cgtgtcccgg cgtcgggcgc tcattcagct tcatgcccgc
     2341 ggaaaacagc gtgcaatagc ctccttcggt gaaacgcgcc gcgcgctggc ccagcctgcc
     2401 atggcgttcg atgtgtccat agcgcacgtt ggcgagttcc tcgtcctctt tccaccactt
     2461 gagccgcttg ggattgattt tctgctcgcc cgggagcagc aggccatcgt gggcatcggc
     2521 aaacgcgcag tagtgggcga attcggccca aagcgcctcg gcggtgtcga gcacggcgtg
     2581 ccggtcgagc tcgatgtcga ttttcggaaa cgccgccttc agttcctcca gcggtcccag
     2641 gaagaggccg aggttcttgt cggcgacgcc ggagccccag atttccttga agcactggtg
     2701 gttcaaccac gcgaggtcgg tctcgcgcgt gcgcttctcc caggtccagt aggcctcgaa
     2761 caccgcggcc tcgcccgccc agtacggggc ggcggcgtcg atcagcctgc gcagacgcgt
     2821 actaagaccc atcgttcgtg ccctgtttca atccgcgtga cgaatgatac actcgacggc
     2881 aaaattgcgg ccgagaccgc agcctgcaga ggagtcccca tgaacgagat caaggaaccc
     2941 gtcgccgcgg gcggccgcat cgacgccatc ctgcccagcc accgcgacct gtattacgac
     3001 ggccagtggc aaaagcccgg gggcggctat caggacaccc tgaaccccgc gacggacagg
     3061 agcctggggc cctgcgcgga agccaacgcg caggacgtgg acgcggcggt ccgggcggcg
     3121 cacaaggcgt tcaagtcctg gcgcgccgtg aagccgctcg agcgcgccgc gctgatgaag
     3181 aaagtcgcgg cggtgctgcg cgccaacgcg gaagaactcg cgatgctgga cgccgccaac
     3241 tgcggcaacc cggtgcgcga aatggtgcgg gatgccggca cggcggccgc gcaatgggat
     3301 ttcttcgccg ggctcgtcac cgaaatgaag ggcgaaacca ttccgatggg cgacggcgtc
     3361 gtcaatctct cggtgcggga gcccttcggc gtctgcgcgc gcatcgtcgc ctacaaccac
     3421 ccgctcatgt tcaccgcggc caaggcctct cccgcgatcg ccgccggcaa tgccgtgatc
     3481 atgaagccgc cgtaccaggc gccgctctcg gcctaccgct tcgtcgaatt gatcgacggc
     3541 atcctgccgc cgggggtgct caacatcctc acctgcggca cggccggcag ccaggcgctg
     3601 gtcgcgcacc cgatggtgcc gcgcgtatcg ctgatcggca gcgtacccac cggacgggcc
     3661 atcatgaagg cggcggccga ccgcctgaag cacgtgtcgc tggagctggg cggcaaaaac
     3721 gcctgcatca tctacccgga cgcggacctc gagaaagcga cgcgcggagc ggtcggcggc
     3781 atgaatttca gctggtgcgg gcagtcgtgc ggctccacct cgcgcctatt catccacgag
     3841 tcggtatacg accgcgtcct ggaaggcgtg ctgaagcacg tccgccatca caagccgggc
     3901 attcccaccg agatggccac caccatgggc gcgatcgtgt cgcggacgca gatggagaaa
     3961 gtcctctcct acatcgagat cggcaagcag gaaggcgcga cgctcgtcta tggcggcaag
     4021 cgccccgacg accccaggct cgccggcggg tttttcgtcg agccgaccgt gtttaccaac
     4081 gtgacgcaga agatgcgcat cgcaacagag gagatcttcg gaccggtgct gtcggtgctc
     4141 aagtggaagg acgaggaaac cctcttcgag caggtcaacg cggtcgagca cggcctgacc
     4201 ggctcggtat ggacaacgag cctcgccaac gcgcaccgcg cggcgagccg catcgagtcg
     4261 ggtttcatct gggtgaacgg cgccgggccg cattacctcg gcgtctcctt cggcggctac
     4321 aagcagtcgg gtatcgggcg cgaggaaagc tacgacgaac tgatgtcgtt cacgcagtcg
     4381 aagaacatca acatcaccct ctaggaaact gccggtccct tgcccgacaa aattcttgcc
     4441 ttgggatctc ctcgctccgg gtcaccgggg agcgagccag ggcgagggat aactcgcggg
     4501 cataccgggc aacctgttac ctgcaggccc ccatttttcc gacttgcact cccttatcgg
     4561 atgcgatctg cgccccctgc accgatgcgg tcataatcac gagctggccg gtaacctcat
     4621 caatagtgac cacgaacact gtttgagtga ccggatccag gtaaataccc ggctccagcg
     4681 tcgtgcccga tgtgaggatc accgtctggc cggtgtccag gttgacatat gaacccggtt
     4741 cgattgtgga cgcgggaata agcggcacta tctggccggt cgttgtctca aggtaaatat
     4801 ttgttacgtt cggtccggca gacgtcggtg tgccggggcc accgagcacg acctcgctgt
     4861 tcggcgggga atcgttggac gaatctattt gagggttggt gatctgatcc ggtacggtcg
     4921 gtagggccgt ggttacgttg accatcgagg cgagatagct ggtggtcatg ggctgcgcgg
     4981 caggcaagtt cgtggccgaa aatactccgc tcacggtgct gccgctctga acgaccgtga
     5041 aagaactgtc tgacggcgga acgaaaccat tgatcagcgc aacattaagc gtgcctccaa
     5101 gcgttgcatt gccggtcacg atgagctgat cgtactgggt gcccgccgcg gcgcccccaa
     5161 tctcaacagc cagcgtgccg ccgggccctt gcgtgtagtt gccgtctatc gtcagcgtac
     5221 cgggtgatgc gccgggtgcg accgtgcccg cagagttatt gacgttgccg acgatcgtgc
     5281 cggtgccgct caggacgccg cccgcgagca cgttcgtcat tcccacgttc acagtaccgg
     5341 cgccgcccag cgtgccgccg gcgagcgtga tcaacggaat ggtggacacg tccgccgacg
     5401 tcgtccccaa gttcagcatc ccgccggaca ccgtcagcgc ttcggcgctc gtgactgact
     5461 tgatgctggt cgtcccgctc gcgctgcggt acgtgatggt gtcggagacg cccggtatgc
     5521 cgatgtccgg aattgtcacc accgcgccgg caaccggcac gacaccctga ttccagttcg
     5581 aagcgacatc ccaattgccg ccgctgccgg ccgaccacct gaaattggcc ggatccggca
     5641 cgatcgtcgc ggcatggact ccgccggtca gcgtgtagtt ggaggcgagg cccgtgccgt
     5701 tgccgagcgc cagcgtcccc gcgctcaacg aataggcgcc caccaacggg ctcgcaacgc
     5761 ttccggcacc acccgcgacc gtgagcgttt cgccgttcac gccgctgacc gagccaaacg
     5821 cggccggtcc gaagctgatg gtaccgtcgt acatccgcgt ccccgccagg ctaatcaccg
     5881 ccggcgtgat ggtgaggctg ccattgacgt agctgatcgt gtagttgccc aggcccgtgc
     5941 ccgtggcatt gctcaggctg tcgttgtaag tgcccacgtt ggtgccgctc gcac
//
LOCUS       DBNGSG010000358         6734 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3175131, whole genome shotgun sequence.
ACCESSION   DBNGSG010000358 DBNGSG010000000
VERSION     DBNGSG010000358.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6734)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6734)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6734
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3175131"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="AC158Q_09275"
     CDS             <1..661
                     /locus_tag="AC158Q_09275"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR3340008.1"
                     /translation="EMFYDQGEPVRAGRMLAAEFVNQGRTYQAVYFQYAEGQGGYYTP
                     EGKNIRKAFLRSPLEFSRISSGFSNRFHPILKQWRAHKGIDYAAPTGTRVKATGDGRV
                     EFAGRHEGGYGNLVVLRHQSKYTTWYGHLSGFAKGIRKGARVAQGDAIGYVGATGLAT
                     GPHLHYEFRINDVHQNPLRVVLPSAPPINPAQKPAFDAAAAPLSQQLELLRNTRFARL
                     D"
     gene            complement(748..1953)
                     /locus_tag="AC158Q_09280"
     CDS             complement(748..1953)
                     /locus_tag="AC158Q_09280"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033922 - peptidoglycan
                     beta-N-acetylmuramidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exo-beta-N-acetylmuramidase NamZ family protein"
                     /protein_id="HIR3340009.1"
                     /translation="MKLGIERLLEEPALRKPLAGRNVALLAHPASVTRDLAHSLDALS
                     AHREIRITAAFGPQHGLRGDKQDNMIESPDYADAIHGIPVYSLYGKVRRPTAEMMDSF
                     DVLLVDLQDLGCRVYTFATTLRYVLEAAAQRGKSVWVLDRPNPAGRPVEGTKLLEGWE
                     SFVGAGPIPMRHGLTLGELARWFVRALRLDVELEVIAMEGWQPEAPPGYGWPIGERAW
                     VNPSPNASNVGMARCYAGTVMLEGTTLSEGRGTTHPLELLGAPDVEARALLAAMHGFA
                     PRWMRGCRLRSCWFEPTFHKHAGKLCGGLQIHVEDAAYDHGTFQPWRLLALALKALRT
                     LAPEYPLWRDFPYEYEHERRAIDLINGGGLLREWVDDRSAAASDLEALAVPDERAWRE
                     ERESLLLYR"
     gene            complement(1950..2630)
                     /locus_tag="AC158Q_09285"
     CDS             complement(1950..2630)
                     /locus_tag="AC158Q_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON-like domain-containing protein"
                     /protein_id="HIR3340010.1"
                     /translation="MKGDFGRLFHFLRGLAAPDERLPGDWRVDPVIGFEPMPPDPVHA
                     VTLNCHPETPTDAVRGIAARVGREPGGKLAVTYILEGDIDRLRVPAPRAPGIAGPLWQ
                     HTCCEVFIARKGMPAYFEFNLSPSGEWAAYAFDGYRSPRTGESRTGALDPQVVVRGAA
                     SQLELDAVIRLDRLPEFQGVPLTLALSAVVEDRAGALSYWALRHPAGKPDFHHSESFA
                     LELGRAGG"
     gene            complement(2648..3001)
                     /gene="erpA"
                     /locus_tag="AC158Q_09290"
     CDS             complement(2648..3001)
                     /gene="erpA"
                     /locus_tag="AC158Q_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HIR3340011.1"
                     /translation="MNAVTETMPEPLVFTSAAAAKVKSLIDEEGNPNLKLRVFVQGGG
                     CSGFQYGFTFDEDAGDDDTRLEKGGVTLLIDPMSLQYLAGAEIDYQQNVEGEQFVIKN
                     PNATSTCGCGSSFSA"
     gene            complement(3043..3453)
                     /locus_tag="AC158Q_09295"
     CDS             complement(3043..3453)
                     /locus_tag="AC158Q_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactofilin family protein"
                     /protein_id="HIR3340012.1"
                     /translation="MFGAKGNNKPQNRIDCLIGAGTTIEGSITFTGGLRVDGRVRGDV
                     ISADGKPGTLVLSEQAQIEGEIRVSHVVVNGTVVGPVHAAEYVELQNKANVTGDVYYK
                     TLEIQLGAVVQGRLVYQNDAKSDKVLQFKPATAE"
     gene            complement(3542..4291)
                     /locus_tag="AC158Q_09300"
     CDS             complement(3542..4291)
                     /locus_tag="AC158Q_09300"
                     /inference="COORDINATES: protein motif:HMM:NF042896.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6776 family protein"
                     /protein_id="HIR3340013.1"
                     /translation="MAQFIRALQRRFGISAPRVAVRTHVAWYWRWLGYFALGTLVFGV
                     GWTTYEYGMELAGFRQSEAARALARLNEEIGRREAQIAELRTQVATAERQMQIERATY
                     GDLARQVKTLSEENSTLKEDLAFFQSLMASGGKEGALTVNRFRVQPESLPGEYRYRLL
                     LVQTGQRVKEFHGSLQFVLNLEQGDRKFVVTLPAEGEKNVKDYQVNFKFFHRVEGTFR
                     VPPGATVKNLQLRVFENGSVAPKLSHTVSVS"
     gene            complement(4301..5341)
                     /gene="argC"
                     /locus_tag="AC158Q_09305"
     CDS             complement(4301..5341)
                     /gene="argC"
                     /locus_tag="AC158Q_09305"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HIR3340014.1"
                     /translation="MARNGIRIGIVGGTGYTGVELLRLLAQHPDCELAAITSRKEAGT
                     RVAEMFHNLRGRVGLKFSDPEKAGLKACDLVFFATPNGVAMEQARALVDGGVRLIDIA
                     ADFRIQDVALWEQWYGVKHACPELVPEAVYGLPEVNRERIREARIVANPGCYPTAVQL
                     GLLPLVEAGVVDLDHLIADAKSGVSGAGRKAEVHTLLAEASDNFKAYGVPGHRHHPEI
                     TQGLVRAAGRPVNLIFVPHLVPMIRGIHATLYARLTGKADLQSLYEKRYANEAFVDVM
                     PAGNHPDTRSVRGANFCRIAVHQPRGGDIAVVLAVIDNLVKGASGQAVQNMNIMFGLP
                     ETAGLEQLALLP"
     gene            complement(5479..5874)
                     /gene="rpsI"
                     /locus_tag="AC158Q_09310"
     CDS             complement(5479..5874)
                     /gene="rpsI"
                     /locus_tag="AC158Q_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HIR3340015.1"
                     /translation="MQRQPHHYGTGRRKTAVARVYMKPGKGDIVVNGKPVDVFFSRET
                     GRMVVRQPLVLTNMLGSFDISVNVDGGGESGQAGAVRHGITRALIEHDASLKSVLKKA
                     GLVTRDAREVERKKVGLHKARKRGQYSKR"
     gene            complement(5885..6313)
                     /gene="rplM"
                     /locus_tag="AC158Q_09315"
     CDS             complement(5885..6313)
                     /gene="rplM"
                     /locus_tag="AC158Q_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HIR3340016.1"
                     /translation="MKTFSAKPHEVQHGWYVVDATGKVLGRLASEIARRLRGKHKPEF
                     TPHVDTGDYIVVVNTEKLRVTGRKAQNKIYHRHSGYPGGIYSTSFAKLQARHPDRVLK
                     LAVKGMLPKGPLGYAMLKKMKLYTGASHPHTAQQPKQLEI"
BASE COUNT         1147 a         2264 c         2106 g         1217 t
ORIGIN      
        1 cgagatgttc tatgaccagg gcgagccggt ccgggcgggc cgcatgctcg ccgcggaatt
       61 cgtcaatcag ggccggacct atcaggcggt ttatttccag tacgccgagg gccaaggcgg
      121 ctattacacg ccggagggca agaacatccg caaggcgttc ctgcgctcgc cgctcgagtt
      181 ctcgcgcatc tcgtccggct tcagcaaccg ctttcacccg atactcaagc aatggcgcgc
      241 gcataaaggc atagactacg cggcccccac cggcacgcgg gtgaaagcga ccggcgacgg
      301 gagagtcgag tttgcggggc gccacgaggg cggttacggc aatctcgtcg tgctccgcca
      361 ccagtcgaaa tacaccacct ggtacggcca cctctccggt ttcgccaagg gcatccgcaa
      421 gggcgcccgc gtcgcccagg gcgacgcgat cggctatgtc ggcgcaaccg ggctcgccac
      481 cgggccgcac ctgcactacg aattccgcat caacgacgtg caccagaacc cgctgcgcgt
      541 ggtgctgcct tccgccccgc ccatcaaccc cgcgcagaag ccggcattcg atgccgctgc
      601 ggcgcccctg tcgcagcagc tggaactcct gcgcaatacc cgattcgcgc ggctggactg
      661 aaccggccgg cgcgacatga ggggaaactc ccctcatgct cctctacagc aggggcgggc
      721 cagttcgttc gttcctgaaa cagcctccta gcgatagagc agcagggatt cgcgctcctc
      781 gcgccacgcc cgctcgtcgg gaacggcaag cgcttcgaga tcggacgccg ccgccgagcg
      841 gtcgtccacc cattcccgca ataaaccgcc accgttgatc aaatcgattg cgcgacgctc
      901 gtgctcgtat tcatacggga aatcgcgcca cagcggatac tccggagcga gcgtgcgcag
      961 tgcctttaag gcgagagcaa gcagccgcca cggctgaaag gtgccgtgat cgtacgccgc
     1021 gtcttcgacg tggatttgca gaccgccaca gagcttgcct gcgtgcttgt ggaacgtggg
     1081 ctcgaaccag caggatcgca accggcatcc tcgcatccac cgcggggcga atccgtgcat
     1141 tgccgccagc aacgcgcgcg cctcgacgtc cggcgcgccg agcaactcca gcggatgcgt
     1201 cgtgccgcgc ccttccgaca acgtcgtgcc ctcgagcatc acggttccgg catagcagcg
     1261 cgccatcccg acgttggacg cgttggggct gggattgacc catgcgcgct cgccaatcgg
     1321 ccagccgtag cccggtggcg cctccggctg ccagccttcc atcgcgatca cctcgagttc
     1381 cacgtccagg cgcagcgcgc ggacgaacca gcgcgccagc tcgcccagcg tgaggccgtg
     1441 acgcatcggg atcggaccgg cgccgacgaa actctcccag ccctcgagca gcttcgtgcc
     1501 ctccaccggg cggccggccg gattgggccg gtcgagcacc cataccgact tgccgcgctg
     1561 agccgcggcc tcgaggacat agcgcagggt ggtggcaaag gtgtaaaccc tgcaacccag
     1621 atcctgcaga tcgaccagca ggacatcgaa gctgtccatc atctccgcgg tggggcggcg
     1681 cacttttccg tacaggctgt acaccgggat gccgtggatt gcatccgcgt agtccggcga
     1741 ctcgatcatg ttgtcctgct tgtcgccgcg cagcccgtgc tggggaccga aggcggccgt
     1801 gatccggatc tcccggtgcg ccgagagcgc gtccagcgag tgcgcaagat cgcgcgtcac
     1861 ggatgccgga tgcgcgagca gcgcgacatt ccggccggcc agcggcttgc gcagcgccgg
     1921 ttcttcgagc aggcgttcga tccccagctt catccgcccg ccctgcccag ttcgagcgcg
     1981 aatgactccg aatgatggaa atccggcttg ccggcagggt gcctgagtgc ccagtacgag
     2041 agcgcgcctg cacgatcttc gaccaccgcc gagagcgcaa gtgtcagcgg cacgccttga
     2101 aactccggaa gccgatcaag gcgtatcacg gcgtcgagtt ccaactggct tgcggcgccc
     2161 cgcaccacga cctgcggatc gagcgctccc gttctcgatt cgccagtccg cggcgaacga
     2221 tatccgtcaa acgcgtacgc cgcccattca ccggagggcg agagattgaa ttcgaagtag
     2281 gccggcatac ccttgcgggc gatgaacacc tcacagcagg tgtgctgcca gagcgggccg
     2341 gcgattccgg gggcgcgagg cgccggcacg cgcaggcggt cgatgtcgcc ctcgagaata
     2401 taggtaacgg cgagcttgcc gccaggttct ctgcccacgc gcgcggctat gccgcgcact
     2461 gcgtcggtgg gagtttccgg atggcagttg agcgtgaccg cgtgtaccgg atcgggcggc
     2521 atcggttcaa agcctatcac agggtccacg cgccaatcgc cgggtagccg ctcgtccgga
     2581 gccgccaagc cgcgcagaaa atgaaaaagg cgaccgaagt cgcctttcac gtgaactcag
     2641 gccccgctca tgccgagaaa gaggacccgc agccgcaggt ggaagtcgcg ttcgggttct
     2701 tgatcacgaa ctgctcgccc tcgacattct gctggtagtc gatttctgcg ccggcaagat
     2761 actgcaggct catggggtcg atcaggagcg tcacgccgcc tttttccagc cgcgtgtcgt
     2821 catcgccggc atcctcgtcg aaggtaaagc cgtactggaa gcccgagcag ccgccgccct
     2881 gcacaaagac gcgcagcttg aggttgggat tgccttcctc gtcgatcagg ctcttcacct
     2941 tggccgccgc ggcgctggta aacaccagcg gttcaggcat cgtttcagtc actgcgttca
     3001 tcaatgaact cctgcaactg ccgttaacgg atcgcggccg ctttactcag cggtagcggg
     3061 cttgaactgg agcaccttgt ccgacttggc gtcattctgg tagacgagcc ggccctgcac
     3121 caccgcgcca agctgaattt cgagcgtctt gtagtataca tcccctgtga cattggcttt
     3181 gttctgcagt tccacgtact ccgcggcgtg gaccgggccg accaccgtgc cgttgactac
     3241 cacgtgggag acgcggattt caccctcgat ctgcgcctgc tcggacagca ccagcgtgcc
     3301 gggcttgccg tcggcggaaa tcacgtctcc acgcacccgc ccgtccacgc gcaggccgcc
     3361 ggtgaaggta atgctgccct cgatggtggt accggcaccg atcaggcagt cgatgcggtt
     3421 ctggggcttg ttgttgcctt tcgctccgaa catggttctg ctccttgcag gccgtttcag
     3481 cttggacgaa acagcccctg ttgttggcga gcaggagggg ggctcccccc ctcatcgccc
     3541 ctcaggaaac actcacggtg tgcgatagtt taggcgcaac cgagccgttt tcaaagaccc
     3601 gcaattgcag gttcttgacc gtcgccccgg gaggcaccct gaaagtgcct tccacgcggt
     3661 gaaaaaactt gaagttcacc tggtaatcct tcacgttctt ctccccctcc gcaggcagtg
     3721 tcaccacgaa cttgcggtcg ccttgctcca gattgagcac gaactgcagc gagccgtgga
     3781 attccttcac gcgctgtccg gtctggacca gcagcaaccg gtagcgatac tcgcccggca
     3841 gcgattccgg ctgcacgcga aagcggttga cggtaagcgc gccttccttg cccccggaag
     3901 ccatcagcga ctggaagaaa gcgaggtctt ccttgagcgt ggagttctcc tcggagagcg
     3961 tcttgacctg ccgggcgagg tcgccgtagg tcgcgcgctc gatctgcatc tgccgctcgg
     4021 cggtcgccac ctgcgtgcgc agctccgcga tctgggcctc gcgccgcccg atttcctcgt
     4081 taagtcgcgc caatgcgcgc gccgcctcgc tctggcgaaa accggcgagt tccattccgt
     4141 attcgtaggt cgtccagccc actccgaaca ccagcgtgcc gagcgcgaag tagcccagcc
     4201 agcgccagta ccatgcgacg tgcgtgcgca ccgccacccg gggcgctgag attccgaatc
     4261 tgcgttgcag cgcgcggatg aattgcgcca ccggcggctc ctagggcagc agcgcgagct
     4321 gctcgagccc ggcggtttcc ggcaggccga acatgatgtt catgttctgc actgcctggc
     4381 cggatgcgcc cttgaccagg ttgtcgatga cggcgagcac caccgcgatg tcccctccgc
     4441 gcggctgatg caccgcgatg cggcagaagt tcgctccgcg caccgagcgc gtgtcgggat
     4501 ggttgccggc gggcatgaca tccacgaacg cctcgttcgc atagcgcttt tcgtagagcg
     4561 actgcaggtc ggccttgccc gtcagccgcg cataaagcgt ggcgtggatg ccgcggatca
     4621 tcggcacgag atgcggcacg aagatgagat tgacggggcg gccggctgcg cgcacgagtc
     4681 cctgcgtgat ttcaggatga tggcggtggc ccggcacgcc atacgccttg aagttgtcgg
     4741 aggcctccgc aagcagcgtg tgcacctccg ccttgcgccc ggccccggag acgcccgatt
     4801 ttgcatcggc aatcaggtga tcgaggtcga cgacaccggc ctcgacgagc ggcagcaggc
     4861 cgagctgcac cgcggtcggg tagcagccgg ggttggccac gatccgcgcc tcgcgaatcc
     4921 gctcgcggtt cacctccggc agtccgtaga ccgcttccgg cacgagttcc gggcaggcgt
     4981 gcttcacgcc gtaccactgc tcccacagcg cgacatcctg gatgcggaag tccgcagcga
     5041 tgtcgatgag tcgcaccccg ccatccacca gcgcgcgcgc ctgttccatg gcgacgccat
     5101 tgggcgtggc gaagaacacc aggtcgcagg ccttgagccc ggccttttcg ggatcggaga
     5161 acttgagccc cacgcgcccg cgcagattgt ggaacatctc ggccactcgc gtgcccgcct
     5221 ccttgcgcga ggtgatcgcg gcgagctcgc agtccgggtg ctgggcgagc aggcgcagca
     5281 gctcgacgcc ggtgtaaccg gtgccgccga cgatgccgat ccggatgccg ttcctggcca
     5341 tggtctccgt tttccgcccg aagtctgctt cagattgact gaaacggacc ccttgcaggg
     5401 gaatctcccc tcattgcgtt agcgcactcc atagacaaca aagccgcccg caggcggctt
     5461 tgtcgcgggt caatgattct atcgcttcga gtactggccg cgtttgcgcg ccttgtgcag
     5521 gcccaccttc ttgcgctcga cctcgcgcgc atcgcgagtc accagtcccg ccttcttgag
     5581 cacggacttg agcgacgcgt cgtgctcgat cagcgcgcgc gtgatcccgt gccgcaccgc
     5641 gccggcctgt cccgactcgc cgccgccgtc cacgttgacc gagatgtcaa agctgccgag
     5701 catattggtg agcaccagcg gctggcgcac gaccatgcgg ccggtctcgc gcgagaaaaa
     5761 tacgtccacg ggcttgccgt tgaccacgat gtcgcccttg ccgggcttca tgtaaacgcg
     5821 cgccaccgcc gtcttgcggc ggccggtgcc gtagtggtga ggctgacgtt gcatcgcggc
     5881 catgctagat ctccagttgt ttcggctgct gcgccgtgtg cgggtggctc gccccggtgt
     5941 acagcttcat cttcttcagc atggcgtagc cgagcgggcc cttgggcagc atgcccttga
     6001 ccgcgagttt cagcacgcgg tccggatggc gcgcctgtag cttggcaaag ctggtgctgt
     6061 agatgccgcc gggatagccg ctgtgccggt ggtaaatctt gttctgcgcc ttcctgccgg
     6121 ttacgcgcaa tttttcggtg ttgaccacga cgatataatc gccggtgtcg acgtgcgggg
     6181 tgaactcggg cttgtgcttg ccgcgcagcc gccgcgcgat ctcggacgcg agccgcccga
     6241 gcaccttgcc ggtggcatcc acgacgtacc acccgtgctg gacttcgtgc ggttttgcgg
     6301 aaaacgtttt catcggtttc tcgacccccg ccgggggcgt tgggaaaggg gcgaattcta
     6361 ttgcaaatca gatgcctgtg tcaaacccgc cgtggcggcc attccgttcc cgccggccgc
     6421 tccagcgcgt gaaaatggcc tagagtccct aacatgatga aacacgcgtg cgttgcgagc
     6481 caaaattggc gatgacgcga aggacggcgc ggggcctagg tcgataggcc caagccggcc
     6541 gacaaagtga gcgccgattt tggccgcaac cccagcgcag ccatttgaat atccaacggt
     6601 aatccaaggg ctggcacgag ttttggccca tggctttgtc gctcgccttg ccaatatggc
     6661 ctatatgggc tgcggctcgc ttcgcgccct gagccaaaat cgtgacagcg cagcgcgcgt
     6721 ttcattatgt tagg
//
LOCUS       DBNGSG010000359         2832 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4532589, whole genome shotgun sequence.
ACCESSION   DBNGSG010000359 DBNGSG010000000
VERSION     DBNGSG010000359.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4532589"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1287)
                     /locus_tag="AC158Q_09320"
     CDS             complement(<1..1287)
                     /locus_tag="AC158Q_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016354885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HIR3340017.1"
                     /translation="MENGPRLQQSRAALTLLALGVVFGDIGTSPLYAVKETFNPAHGI
                     ALTTANILGGLSTIFWALMIVVSLKYVLLIMRADNRGEGGIMALTALASAAVKGDPRR
                     RAIIVALGLLGASLFFGDAVLTPAISVLSAVEGLEIGTSALKPYVVPVSVGVLIALFL
                     FQRKGTAAVGALFGPVCVLWFLALAAVGLYDITLNPSVLQALNPVHALGFATQHGFAS
                     FVVLGAVLLAFTGAEALYADMGHFGRGPIRLAWFGLVAPALVLNYFGQGALLIANPQA
                     IVNPFYLAFPSWALYPMVALATAATVIASQATISGAYSITRQAIQLGFLPRMNIVQTS
                     ASTFGQIYIPGVNWLLLVMVLAAVVGFGSSSQLASAYGVAVTGTMMVDTVLTFFVIRY
                     GWGYNPLLCVLATGFFFIVDAAFFSSSLLKVAEGGRF"
     gene            1553..2314
                     /locus_tag="AC158Q_09325"
     CDS             1553..2314
                     /locus_tag="AC158Q_09325"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3340018.1"
                     /translation="MVFRTLRPLALMLSWMMWLAPLSAFAQADYAREQRWADEIQPAI
                     VVGDPVYLALKSGRKFLAIHAPSAGAAAGVVVVHGLGVHPDWGLINTLRSQLAEQGYA
                     TLSVQMPVLMAEMRADSYPPLFPEAAERLAAAVAFLRGKGLKRVAIVSHSLGSRMVNY
                     FLNHAGRTRVDAWVSIGILGEFTQPESFSTPVLDLYGEKDFPAVLDHAVQRAAAIRGI
                     HGSGQVQVAGADHFFTGMEGNLVRQVRAFLDRRLK"
     gene            complement(2398..>2832)
                     /locus_tag="AC158Q_09330"
     CDS             complement(2398..>2832)
                     /locus_tag="AC158Q_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR3340019.1"
                     /translation="MWIGNALLVVLNLPLVGLWVQLLKVPYRWLFPSIIMFCAVGNYS
                     INNSAMDLYLVGIMGILGYVLVKLECPPAPLILGFVLGPLMEENLRRALLISRGDPTV
                     FFTRPISLGFMIASVLILITMILPAIRKKKDQAIMAGGGDAL"
BASE COUNT          539 a          896 c          962 g          435 t
ORIGIN      
        1 gaaccggccg ccctcggcga ccttgagcag gctcgacgag aagaacgcgg cgtccacgat
       61 gaagaaaaag ccggtggcca gcacgcacag cagcgggttg tagccccagc cgtagcggat
      121 cacaaagaag gtgagcacgg tgtccaccat catggtgccg gtgaccgcga cgccgtaggc
      181 cgaagcgagc tgggaggagg agccgaaacc caccacggcg gcgagcacca tgacgagcag
      241 cagccagttg acgcccggga tgtagatctg gccgaacgtg ctcgccgagg tctgcacgat
      301 gttcatgcgc ggcagaaagc ccagctggat cgcctgccgg gtgatcgagt acgcgcccga
      361 aatggtggcc tgggaggcga tcacggttgc cgccgtcgcg agcgccacca tcgggtagag
      421 cgcccacgag gggaaggcga gatagaacgg gttgacgatg gcctgcgggt tggcgatcag
      481 cagcgcgccc tgcccgaaat agttcagcac cagcgccggg gcgaccagcc cgaaccaggc
      541 gaggcggatc gggcccctgc cgaaatgccc catgtcggcg tagagcgcct ccgcgccggt
      601 gaacgcgagc agcacggcgc ccagcacgac aaaggaggcg aagccatgct gcgtcgcgaa
      661 acccagggcg tggacaggat tgagcgcctg cagcaccgac gggttgagag tgatgtcgta
      721 gagcccgacc gccgccagcg cgagaaacca gagcacgcag accgggccga acagcgcgcc
      781 cacggccgcc gtgcccttgc gctggaacaa gaacagcgcg atcagcacgc cgaccgagac
      841 cggcacgacg tagggcttga gcgcgctggt gccgatctcc aggccttcca ccgcggacag
      901 caccgagatg gcgggcgtca acacggcatc gccgaagaag agcgatgcgc ccagcagccc
      961 gagcgcgacg atgatcgcgc ggcggcgcgg gtcgcccttc accgccgccg aggcgagcgc
     1021 ggtgagcgcc atgatcccgc cctcgccgcg gttgtcggcg cgcatgatca gcagcacgta
     1081 cttgagggac accacgatca tcagcgccca gaagatcgtg gacagcccgc ccaggatgtt
     1141 ggcggtggta agcgcgatgc cgtgcgcggg gttgaacgtt tccttgacgg cgtagagcgg
     1201 gctggtgccg atgtcgccga agacgacgcc gagcgcgagg agcgtcagcg cggcgcggga
     1261 ttgctgaagg cgcggtccgt tttccatcct gtttctctca ccaatgagag ggcgcgaatc
     1321 ctactctcgc accgcaccag ccgcaattga tcctgttgta atttaactac atcattcata
     1381 atcaaacagt tgctacgtta aatatgacac ggacaatgtc gatatagtgc attgcaacaa
     1441 tccatgttcg gtccggtacg ctctaccctg ggcctgcagc gtcccgcgcg ggatgcgccg
     1501 ggcagtgcat tttgatactt tcgatgcgac accggctgga tgaaggagcg gaatggtttt
     1561 caggacgctg cgcccgctgg cgctgatgct gtcgtggatg atgtggctcg cgccgttgtc
     1621 cgcgttcgcg caggcggatt acgcgcgcga gcagcgttgg gcggacgaga ttcagccggc
     1681 gatcgtggtg ggcgacccgg tttacctggc gctaaaatcc gggcggaaat ttctcgcgat
     1741 ccacgcgccc agcgccgggg cggcagccgg cgtcgtcgtc gtgcacgggc tcggcgtgca
     1801 tcccgactgg ggcttgatca acacgctgcg cagccagctc gcggagcagg gctacgccac
     1861 gctctcggtg cagatgccgg tgctcatggc ggaaatgcgc gccgattcct atccgccgct
     1921 gtttcccgaa gccgcggagc ggctcgccgc cgcagtggct ttcctgcgcg gcaagggact
     1981 gaagcgggtg gcgatcgtgt cgcacagcct gggctcgcgc atggtcaatt attttcttaa
     2041 ccacgcgggc cgcacccgcg tcgatgcctg ggtctcgatc ggcattctcg gcgagttcac
     2101 ccagccggag tcgttcagca cgccggtgct ggacctctac ggcgagaagg attttcccgc
     2161 agtgctggac catgccgtgc agcgcgcggc ggcgatccgc ggcatccacg gctcgggcca
     2221 ggtgcaggtc gccggcgccg accatttctt caccgggatg gaagggaacc tcgtgcgcca
     2281 ggtgcgcgca ttcctggacc ggcgcctgaa ataaccgtcc ctcgccagaa gctgctcacc
     2341 gcgaacgaat taaaaaaggg gggcgtagcc ccccttttcc catagcagcg cggccggtca
     2401 gagcgcgtcg ccgccgccgg ccatgatcgc ctggtccttc ttcttgcgga tggccggcag
     2461 gatcatggtg atcaggatca gcacgctcgc gatcatgaat cccaggctga tcgggcgcgt
     2521 gaagaacacc gtgggatcgc cgcgcgagat cagcagcgcg cgccgcaggt tctcctccat
     2581 cagcggcccc agcacgaagc ccaggatgag cggcgccggc gggcactcga gcttcacgag
     2641 gacgtagccc aggatgccca tgatgccgac gaggtagaga tccatcgcgc tgttgttgat
     2701 gctgtagttg cccaccgcgc agaacatgat gatggagggg aacagccagc ggtaggggac
     2761 cttgagcagc tggacccaga ggccgaccag cggcaggttg aggaccacca gcagcgcgtt
     2821 gccgatccac at
//
LOCUS       DBNGSG010000360         2480 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4193906, whole genome shotgun sequence.
ACCESSION   DBNGSG010000360 DBNGSG010000000
VERSION     DBNGSG010000360.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4193906"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /gene="lolB"
                     /locus_tag="AC158Q_09335"
     CDS             <1..403
                     /gene="lolB"
                     /locus_tag="AC158Q_09335"
                     /inference="COORDINATES: protein motif:HMM:NF015512.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HIR3340020.1"
                     /translation="HLAEQDEIWLLTPTGQALAHIVSGPAGATLTGADQNVYHAADAE
                     SLTRRALGWELPVTRLTWWVRGEVVPQGPVEETLRDQQGRLVSLQQDGWHVTLVHPAA
                     NGQGGRPQRLDLTRPGYRIRLVIDNWRPDSQ"
     gene            414..1259
                     /gene="ispE"
                     /locus_tag="AC158Q_09340"
     CDS             414..1259
                     /gene="ispE"
                     /locus_tag="AC158Q_09340"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HIR3340021.1"
                     /translation="MGGTLDFPAPAKLNLMLRVTGRRADGYHLLQTVIRFIDYGDTLR
                     LRVRDDGAITRTNEIAGIPADEDLAVRAAQLLQRAAGTRLGADITLEKRIPVGGGLGG
                     GSSDAATALLALNHLWRAGLPRERLAALALELGADVPVFVGGENALAEGIGERLTPLN
                     LAPAWYLVLTPPTAVPTARIFADSQLKRDSKPIKITAFSAEQAGNDLEPVVCREYPEV
                     ARHLAWLRRAAPAWVTGTGACVFAAFPTEGAARQVWKQAPEGMQGFIAQGLAQHPLRD
                     LARTG"
     gene            1327..1400
                     /locus_tag="AC158Q_09345"
     tRNA            1327..1400
                     /locus_tag="AC158Q_09345"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1361..1363,aa:Gln,seq:ttg)
     gene            1492..2424
                     /locus_tag="AC158Q_09350"
     CDS             1492..2424
                     /locus_tag="AC158Q_09350"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HIR3340022.1"
                     /translation="MVFTGNANPRLAEDVAAHLKVRLGRAKVGRFSDGEVMVEILENV
                     RGRDVFVLQSICSPTNDNLMELLVMVDSLKRASAGRITAAIPYMGYARQDRRPSSARV
                     PITAKVVANMMSSVGVDRVLTMDLHSEQIQGFFDIPVDNIYSGPIMLGDVWKHGYKNL
                     VVVSPDVGGVLRARALAKRLESDLAIIDKRRPRPNVATVMNIIGEVEGRTCVIIDDLV
                     DTANTLCEAAAALKERGAERVLAYCTHPVLSGKAVERIAGSALDEIVVTDTIPLREDA
                     KTCRKIRVLTVSGLLAETMRRISNEDSVSSLFVE"
BASE COUNT          431 a          771 c          873 g          405 t
ORIGIN      
        1 gcacctcgcg gaacaggatg aaatctggct gctgaccccg acgggacagg cgcttgcgca
       61 catcgtgtcc ggcccggcgg gcgccacgct gaccggcgcc gatcagaacg tgtatcacgc
      121 cgccgacgcc gaaagtctca cgcgccgggc cctgggctgg gagttgccgg tgacgcgtct
      181 cacgtggtgg gtgcggggcg aagtcgtgcc gcaggggccg gtcgaggaaa cgctgcgcga
      241 ccagcagggc cgcctggtca gcctgcagca ggatggctgg cacgtcactc tggttcatcc
      301 cgccgcaaac ggccagggcg gccggccgca gcggctggac ctcacgcgcc ccggctatcg
      361 gatccggctg gtgatcgaca actggcggcc ggactcgcaa tgaagtgcgc gtaatgggcg
      421 ggacactcga ctttcccgcg cctgccaagc tcaacctgat gctgcgggtc acgggccggc
      481 gcgccgatgg ctatcacctg ctgcagacgg tgatccgctt catcgactac ggcgacacgt
      541 tgaggctgcg tgtgcgcgat gacggggcga tcacgcggac aaatgagatt gcgggaatcc
      601 ccgccgacga ggaccttgcc gtgcgcgcgg cgcagttgct gcaacgggcc gccggcacgc
      661 ggctgggagc cgatatcacg ctggagaaac gcataccggt cggcggcggg ctcgggggcg
      721 gcagctcgga cgccgccact gcgttgctcg cgctgaacca cttgtggcgc gccgggctcc
      781 cgcgcgagcg cctggcggcg ctggcccttg aactgggggc cgatgtgccg gttttcgtgg
      841 gcggggagaa cgcgctggcc gaaggcatag gggagcgcct gacccccctc aacctggccc
      901 cggcctggta tctcgtcctg accccgccga cggcggtgcc gacggcccgg atttttgctg
      961 attctcaatt gaaaagggac tcaaaaccca ttaaaatcac ggccttttcg gcagaacagg
     1021 ccggcaacga tctggagccc gtcgtctgcc gcgagtaccc ggaggtcgcg cggcacctgg
     1081 cctggctcag gcgggcggcg ccggcctggg tgaccggcac gggggcctgc gtatttgcgg
     1141 cgttcccgac cgaaggcgca gcgcgccagg tttggaagca ggcgcccgag ggcatgcaag
     1201 ggttcatcgc gcagggattg gcgcagcacc cgctgcgcga tctggcgcgc actgggtgag
     1261 agctgactgg aagataactg agggcaactg aggacacgct ggggcgggct cgggatctgg
     1321 aactgtaggg gagtcgccaa gctggttaag gcaccggatt ttgattccgg catgcgaagg
     1381 ttcgaatcct tcctcccctg cccactggtt gacgtaaacg gcttcaccga ggaaatcgag
     1441 gacgagatgc tgcagggacg taacagccag agcacggcct ttgaccgcct gatggtgttc
     1501 accggcaatg ccaatccccg gctcgccgag gacgtcgccg cgcacctgaa agtccggctc
     1561 gggcgcgcca aggtcggccg cttcagcgac ggcgaggtga tggtcgagat cctcgagaac
     1621 gtgcgcgggc gcgacgtgtt cgtgctgcag tcgatctgct cgccgaccaa cgacaacctg
     1681 atggagctgc tggtgatggt ggattcgctg aagcgcgctt ccgcggggcg gatcaccgcc
     1741 gcgattccct acatgggata cgcgcggcag gaccgccgcc cgagttccgc gcgggtaccg
     1801 atcactgcca aggtcgtcgc caacatgatg tcgagcgtgg gcgtggaccg cgtgctcacc
     1861 atggacctcc actcggaaca gatccagggg ttcttcgaca tcccggtgga caacatctac
     1921 tcgggaccga tcatgctggg cgatgtgtgg aagcatggat acaagaacct ggtcgtggtg
     1981 tcgcccgacg tcggcggcgt gctgcgggcc cgggcactcg cgaagcggct ggaatccgac
     2041 ctcgcgatca tcgacaagcg ccgtccgcgg cccaacgtcg ccacggtgat gaacatcatc
     2101 ggcgaggtcg aaggccgcac ctgtgtcatc atcgatgacc tggtggatac cgccaacacg
     2161 ttgtgcgaag cggcggcggc gctcaaggag cgcggtgcgg agcgcgtgct cgcctactgc
     2221 acgcacccgg tgctctcggg gaaagcggtg gagcgcatcg cgggctcggc gctcgacgag
     2281 atcgtggtga ccgataccat tccgctgcgc gaggacgcga agacgtgcag gaagatccgc
     2341 gtgctcacgg tctcgggcct gctggccgag accatgcgcc gcatcagcaa cgaagactca
     2401 gtcagctcgc tatttgttga atagcgagct gagtgatgag ttttgagtga tgagttttga
     2461 ggtgaacagc gctttttaac
//
LOCUS       DBNGSG010000361         5280 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_288281, whole genome shotgun sequence.
ACCESSION   DBNGSG010000361 DBNGSG010000000
VERSION     DBNGSG010000361.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5280)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5280)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5280
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_288281"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="AC158Q_09355"
     CDS             <1..786
                     /locus_tag="AC158Q_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340023.1"
                     /translation="FFYDGMYWVYHDDNWYASSWYNGPWGLVAPQYVPYFVLRIPVRY
                     YRQPPPYFRGWRADAPPRWGDHWGRDWERRRAGWDRWDRRSVPAPAPLPVYQRQYSGD
                     RYPRAEQQRELHGQHYRYQPRSAAVRQHYQERAVQRAPESSQRGQPGEPQPRGSRPQA
                     NDRSASPPPSQREPQGKGASQEQSRGQGRDRDPEPQGKGASQDQIRGQGQDKGREPQG
                     KGASQEKGRGQGQDKAREPQGKGESQEQRRGQGQDRDRDREKG"
     gene            complement(966..>5280)
                     /locus_tag="AC158Q_09360"
     CDS             complement(966..>5280)
                     /locus_tag="AC158Q_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GH36-type glycosyl hydrolase domain-containing
                     protein"
                     /protein_id="HIR3340024.1"
                     /translation="VPGLGLKRGLAEDLVIAPYASALALMVAPAAACANLQRLADEGL
                     AGKFGLYEAIDHTPSRLPRGNSSVVVRSFMAHHQGMSLLALGYQLLDRPMQKRFESDP
                     RFQATALLLQERVPRAAVFHMHAAGFPTMRAAESAPEMPVRVLASPNTPIPEVQLLSN
                     GRYHVMVTNAGGGYSRWKDLAVTRWREDTTCDNWGMFCYLRDVASGVFWSNTHQPALK
                     AAESCEAIFSEGRAEFRRRDLDYDTHTEIVVSPEDDIELRRVHIINRARVRRTIEVTS
                     YAEVVLAAPAADALHPAFSNLFVQTEIIHERRAILCTRRPRSLAEQAPWMFHLMAVRG
                     AEAGEISYETDRMRFIGRGRTVAAPQAMRETAALSGTAGSVLDPIVAIRHRITLDPEQ
                     AATIDMVSGIGATRDAALGLAGKYQDRYLADRVFELAWTHSQVVLRQINATEADAQLY
                     GRLAGSVIYANSSLRAEAGVLGRNRRGQSGLWGYAISGDLPIVLLQIGDLANIDLVRQ
                     LVQAHAYWRLKGLAVDLVIWNEDHAGYRQLVQEQIMGLIAAGIEAHVMDRPGGIFVRR
                     AEQISDEDRILVQSAARAIINDRRGALGDQINRRSLMEPRAPHLNMPRLKPARAPRID
                     SPKAAAPPQRELLFYNGLGGFTLDGREYVITTAHGQVTPAPWVNVLANPHFGTVISES
                     GMAYTWSENAHEFRLTPWSNDPVTDASGEAFYLRDEDSGDFWSPAPLPARGATPYVSR
                     HGFGYSSFEHAEGGIRSELTVYVAPDAAVKFSVLKVRNESGRERRLSAAGYVEWVLAD
                     LRPKAAMHVITEIDPASGALLARNAYNTEFADRVAFFDIDVTARTFSGDRAEFVGRNG
                     TLADPAAMTRSQLSNKVGAALDPCAAIQAAFELADGEEREFIFRLGAGRNLDDARKLL
                     ARFRGPAAARDALEQVRQYWRHTLGAVQVETPDPSLDVLTNGWLLYQTLACRLWARSG
                     YYQSGGAFGFRDQLQDVMALIHAEPGLVREHLLRCAARQFREGDVQHWWHPTSGRGVR
                     THCSDDYLWLPLAMCRYVLGTGDAAVLDESIPFLDGRALNPEEDSYFDLPRRSEESAS
                     LYQHGVRAVLRGLNFGERGLPLIGSGDWNDGMNIVGAQGKGESVWLGFFLYEVLMRFT
                     EIARMRSDMSFAERCQREAAQLRQNIESSGWDGEWYRRAYFDDGTPLGSAGNTECQID
                     SIAQSWSVLSGAGDPRRSRMAMAAVDRRLVRRDHGLVQLLDPPFDKSALNPGYIKGYV
                     PGVRENGGQYTHGAIWTVMAFAALGDSRRAWELFDMINPLNHASSEAAIATYKTEPYV
                     AAADVYAVAPHTGRGGWTWYTGSAGWMYRLIVESLLGLKLETDKLRFAPCVPADWESF
                     RLHYRYRETAYHITVFQEHAADGRTRVSIDGVEQKNEALLLVDDRAEHAVEVRIHRAQ
                     S"
BASE COUNT          880 a         1736 c         1814 g          850 t
ORIGIN      
        1 ttcttctacg atggcatgta ctgggtctac catgacgata actggtacgc gagctcgtgg
       61 tacaacgggc cgtgggggct cgtggccccc cagtacgtgc cgtatttcgt cctgcggatc
      121 cccgtgcgct attaccggca accgcctccg tacttccgcg ggtggcgggc ggatgcgccg
      181 ccgcgctggg gtgatcactg gggccgtgac tgggaacggc gtcgggcagg atgggaccgg
      241 tgggaccgaa ggtctgttcc cgcgcccgcc ccgctgcctg tctaccagcg gcagtattca
      301 ggggatcggt atccccgggc ggagcagcag cgggaacttc acggccagca ttaccggtat
      361 cagccgcgtt ccgctgctgt gcgacagcat taccaggagc gcgcagtgca acgagcgccc
      421 gagtcttctc agcggggaca gccgggagag ccgcagccga gagggtccag gccgcaggct
      481 aacgaccgtt ctgcctcgcc accgccgtcc cagcgcgagc cgcaaggcaa gggcgcgtca
      541 caggagcaga gtcgcgggca aggccgggac agggaccccg agccgcaggg caagggtgcg
      601 tcgcaggatc aaattcgcgg tcaagggcag gacaagggcc gcgagccgca aggcaagggt
      661 gcatcgcagg agaaaggccg cgggcagggg caggacaagg cccgcgagcc gcaaggcaag
      721 ggcgagtcgc aggagcaaag gcgcgggcaa gggcaggaca gggaccggga ccgcgaaaaa
      781 ggttgaggaa cgaggtcggg aacgcaacgg gtaacacgcg ggccagaggg catccgcccg
      841 aagcgacagc ccgcgcaaag gcggatcggg cacggcgggc acctgcccgg gatccggaac
      901 gggaaacagg acgcggcctg cgaagaagaa tcggcgtaca caggctgttt caacctcggg
      961 cggcttcagc tttgagcccg gtgaattcgg acctcgactg cgtgttccgc gcgatcgtcg
     1021 accaggagca gggcttcgtt cttctgctcg acgccgtcaa tcgatacacg ggtcctgccg
     1081 tcggcagcgt gttcctgaaa gacggtgatg tggtaggccg tttcacggta gcggtaatgc
     1141 agcctgaacg attcccaatc ggccggaaca cacggcgcaa agcgcagctt gtcggtttcg
     1201 agcttcaacc ccagcaggga ctccacgatt aaccggtaca tccagccggc ggacccggtg
     1261 taccaggtcc agccgccgcg gccggtgtgc ggcgcaaccg cgtagacgtc ggccgcggcc
     1321 acgtaaggct ccgttttgta ggtggcgatt gcagcctcgg aactcgcatg gttcagcggg
     1381 ttgatcatgt cgaacagctc ccatgcgcgc cggctgtcgc ccagggcggc aaacgccatc
     1441 accgtccaga tggccccgtg ggtgtactgg ccgccatttt cgcgcacgcc gggaacgtaa
     1501 cccttgatgt agccgggatt caaggccgac ttgtcgaacg gcggatccag cagctggacc
     1561 agcccgtggt cgcgccgcac gaggcgccgg tccactgccg ccatcgccat gcgcgagcgc
     1621 cgtgggtcgc ccgcgccgga cagaaccgac cagctttgcg cgatcgagtc gatctggcac
     1681 tcggtgttgc ctgccgatcc cagcggcgtg ccgtcgtcga agtaggcgcg ccggtaccac
     1741 tcgccatccc agccgctgct ctcgatattc tggcgcaatt gcgcggcctc tctctggcag
     1801 cgttccgcga aggacatgtc gctgcgcatg cgcgcgatct cggtgaatcg catcagcacc
     1861 tcgtacagga agaaacccag ccacacgctt tcgcccttgc cctgcgcgcc cacgatgttc
     1921 atgccgtcgt tccagtcgcc ggagccgatg agcggcaggc cgcgctcgcc aaagttgagg
     1981 cctctcagga cggcgcgcac gccgtgctgg tacaggctgg ccgattcttc cgagcggcgg
     2041 ggcaggtcga aatacgaatc ctcttcgggg ttcagcgccc ggccgtcgag gaagggtatg
     2101 gattcgtcca gcaccgcggc atcgccggtg cccagaacgt agcggcacat cgccagcggc
     2161 agccacaggt agtcgtcgga acagtgcgtg cgcacgccgc gaccggacgt gggatgccac
     2221 caatgctgga cgtcgccttc ccggaactgg cgggccgcgc agcgcagcag gtgttcgcgc
     2281 acgagcccgg gctcggcatg gatcagcgcc atcacgtcct gcagctggtc gcggaaaccg
     2341 aacgcgccgc ccgattggta gtatccgctg cgcgcccaca ggcggcaggc cagggtctgg
     2401 tacaagagcc agccgttggt cagcacgtcg agggacgggt cgggcgtttc cacctgcact
     2461 gcgcccagcg tgtgccgcca gtattggcgc acttgttcca gcgcgtcgcg cgccgcggcc
     2521 gggccgcgga agcgcgcgag cagcttgcgt gcgtcgtcga ggttgcgccc ggcgccgagc
     2581 ctgaagatga actcgcgctc ttccccgtcg gccagctcga acgcggcctg gatggccgcg
     2641 cagggatcca gggcggcccc caccttgttg gaaagctgcg agcgggtcat ggccgccgga
     2701 tccgcgagcg tgccattgcg cccgacgaac tcggcgcggt cgccgctgaa ggttcgggcc
     2761 gtgacgtcga tgtcgaagaa cgcaacccgg tcggcgaact cggtgttgta ggcattgcgc
     2821 gcgaggagcg cgccgctcgc cggatcgatc tcggtgatca cgtgcatggc cgctttcggc
     2881 cgcaggtccg cgagcaccca ttcgacgtat cccgcggcgg agagccggcg ctcgcggcct
     2941 gactcgttgc gcactttcaa taccgagaac ttgactgcgg cgtccggagc cacgtaaacc
     3001 gtgagctccg agcggatgcc gccttccgcg tgctcgaagc tgctgtaacc gaaaccgtgc
     3061 cggctgacgt agggcgtggc gccgcgggcg ggcaatggcg cgggcgacca gaagtccccg
     3121 ctgtcctcgt cacgcaggta aaaggcctcg ccgctcgcgt ctgttaccgg atcgttgctc
     3181 cagggggtca ggcggaactc atgggcgttc tcgctccagg tgtaggccat gccgctctcg
     3241 gagatgaccg tcccgaagtg cggattcgcc agcacgttca cccagggtgc cggcgttacc
     3301 tgcccgtgag cggtcgtgat gacgtactcg cgcccatcga gggtgaagcc gccgagcccg
     3361 ttgtagaaga gaagttcgcg ctgcgggggc gcggcagcct tcggggagtc gatgcggggc
     3421 gcgcgggccg gcttcaggcg cggcatgttc agatgcggcg cgcgcggttc catcaggctg
     3481 cgccgattga tctggtcccc cagcgctccg cgccggtcgt tgatgatggc acgcgcggcc
     3541 gactggacca gaatgcggtc ttcatccgat atctgttcgg cccgccgcac gaagattccc
     3601 ccgggccggt ccatcacgtg ggcttcgatg cccgcggcga tcagccccat gatctgttcc
     3661 tgcaccagtt gccggtaacc ggcgtgatct tcgttccaga tgaccaggtc cacggcgagt
     3721 cccttcaggc gccagtaggc gtgggcctgc acgagctggc gcaccaggtc gatgttcgcg
     3781 aggtcgccga tctgcagcag cacgatcggc agatcgccgg atatggcgta gccccacaag
     3841 ccggattgtc cgcggcggtt tctgccgaga acgcccgctt ccgcgcgcag gctggaattg
     3901 gcgtagatga cggagcccgc caggcgccca taaagttgcg cgtcggcctc ggtcgcattg
     3961 atctgccgca gcaccacctg gctatgggtc cacgccagct cgaaaacccg gtccgcgagg
     4021 taccggtcct ggtattttcc ggccaggccc agggccgcgt cgcgggtggc gccgatgccg
     4081 gagaccatgt ctatcgtggc cgcctgttcc ggatcgaggg tgattcgatg ccggatcgcg
     4141 acgatcggat ccagcaccga gcccgcggtt cccgagagcg ccgcggtttc tctcatcgcc
     4201 tgcggcgcgg caacggtgcg tccgcggccg atgaaacgca tgcggtccgt ttcgtacgaa
     4261 atctctccgg cctccgcccc gcgcacggcc atcaggtgga acatccacgg cgcctgctcg
     4321 gcgagcgagc gcggccggcg cgtgcacagg atggcccgcc gctcgtggat gatctcggtc
     4381 tggacgaaaa gattgctgaa cgccgggtgc aacgcgtccg cggcgggcgc ggcgagcacc
     4441 acttcggcgt aactcgtgac ctcgatcgtg cgccggaccc gcgcgcggtt gatgatgtgc
     4501 acccggcgca gctcgatgtc atcttccggc gaaacgacga tctcggtgtg cgtgtcgtaa
     4561 tcgaggtcgc gccgccggaa ttccgcgcgt ccctccgaaa aaatcgcctc gcaggattcc
     4621 gctgccttga gcgccggctg gtgggtattc gaccagaaca cgccgctcgc cacgtcgcgg
     4681 aggtaacaga acatgcccca gttgtcgcag gtcgtgtctt cgcgccagcg cgtgacggcg
     4741 aggtctttcc agcggctgta gccaccgccg gcgttcgtga ccatcacgtg gtagcggcca
     4801 ttcgacagca actgcacttc cggtatcggc gtgttgggac tcgccagcac gcgcaccggc
     4861 atttcggggg cgctttcagc ggcgcgcatc gtggggaacc cggcggcgtg catatgaaac
     4921 acggcggccc tcggcacccg ttcctgcaac agcaaggcag tggcctggaa ccgcgggtcg
     4981 gattcgaatc gcttctgcat cgggcggtcg agcagctgat aaccgagcgc aagcaggctc
     5041 atgccctggt gatgggccat gaatgaacgc accacgacgc ttgagtttcc gcgcggcaga
     5101 cgtgacggag tgtggtcaat ggcttcatac aggccgaatt ttccggccag tccctcgtcc
     5161 gcgagccgct gcaggttcgc gcacgccgcc gcgggcgcca ccatcagggc gagcgccgac
     5221 gcgtacgggg caatgaccag atcctcggcc agcccgcgct tgagccccag gccgggaacg
//
LOCUS       DBNGSG010000362         8484 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2665220, whole genome shotgun sequence.
ACCESSION   DBNGSG010000362 DBNGSG010000000
VERSION     DBNGSG010000362.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 8484)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8484)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8484
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2665220"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="AC158Q_09365"
     CDS             complement(<1..354)
                     /locus_tag="AC158Q_09365"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3340025.1"
                     /translation="MKTLLVPVDGSENSFRALDYAVSQSRYCPVTLHLLNVEPPLDDY
                     GMVRAHLSRQQHSRLMRERAKAILKRAATRVRSPRVACRTHVVVGDVAATIAGAARRY
                     RCESIVMGTRGMGRLA"
     gene            566..1555
                     /locus_tag="AC158Q_09370"
     CDS             566..1555
                     /locus_tag="AC158Q_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3340026.1"
                     /translation="MSLLNLSIATTDYDHFRDFRLGYVKAEGIDLNWLNLGHHECFAR
                     FTANREFDLSELSLAKFAAQVTREGADIIGLPVVCSRLFRFSSFYVNKKSRIRTVKDL
                     AGRKVGSPEWAHSAAVYMRGWMHNDMGVKLADVHWYQAGANSPGREEKIELNLPRGVK
                     LTRVADKSLSQLLAAGEIDCAIIARPPTCFLEDHPDVERLFPNYHEMEEEYYERTRVW
                     PIMHIIAMRKSVLEDHPWVARNLYNAFLESKRRSIERLLDPAVSRYPLAWLPTYARTM
                     REMFGGDPFPYGIEENRATWEQMALYTWQQGIAHRPFKPEEMFPEGLMTRVVI"
     gene            1584..2330
                     /locus_tag="AC158Q_09375"
     CDS             1584..2330
                     /locus_tag="AC158Q_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012804.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR3340027.1"
                     /translation="MSATPKSGGGVPAALISDLAAASRILAAQGVVDGFGHVSMRHPQ
                     AHDRYFMSRSIAPALVTPGDLIEYDLDSDPCNANGRNSFLERFIHGEIYKARPDVMSV
                     VHSHSPSVIPFGLVAVPMQAMFHNAAFLAAGVPVFDIKKQFGATDLLVSDGAKGVALA
                     ESLGEKDIAMMRAHGSVTCGPTLQTAVFRAVYTEVNARIQHWTLALAGGKPLAVLDVE
                     EGRLADVINQTAGMRAWDLWRTQVRAQVGW"
     gene            2357..3031
                     /locus_tag="AC158Q_09380"
     CDS             2357..3031
                     /locus_tag="AC158Q_09380"
                     /inference="COORDINATES: protein motif:HMM:NF012804.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR3340028.1"
                     /translation="MSETDVLKQQLVDCIRMLERSDIIDYNGHASIRAGDNRMFINAG
                     SCQRSLLTAGDICTIDFDGNVIDGNGKPPLEFHLHAGIYKSRSDVKAVVHAHPKWSTF
                     LTMVGESYQPVYAQGSLVWPMPVLDSPNSINNPAMAKRLADTLGDRPAAMMKSHGAVT
                     VGKDIVEAFVLANYLEENAYRQYMALQIGKPYSFSDKEVALCREKLWTESLFRRTWDH
                     FRAKLD"
     gene            3148..3330
                     /locus_tag="AC158Q_09385"
     CDS             3148..3330
                     /locus_tag="AC158Q_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340029.1"
                     /translation="MKATAKSEGRQGIAPRAARKKRFRSFWRRIQGIFGSPNSNGVSN
                     NSLGVAFAFLCVAFDL"
     gene            complement(3417..3698)
                     /locus_tag="AC158Q_09390"
     CDS             complement(3417..3698)
                     /locus_tag="AC158Q_09390"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF036850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase family protein"
                     /protein_id="HIR3340030.1"
                     /translation="MAVNATSQGMVGQPALDIRLDRLPKSALAADIIYIPLETPFLAA
                     ARARGNRTVNGLGMLLHQGRPAWKAWFGIDVEVTPGLRALMEKSIRDDS"
     gene            complement(3825..4271)
                     /locus_tag="AC158Q_09395"
     CDS             complement(3825..4271)
                     /locus_tag="AC158Q_09395"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020090.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase family protein"
                     /protein_id="HIR3340031.1"
                     /translation="MAHPDLFLLAGVMGWPVMHSRSPLMHNYWFRQRHLAGTYVPLAI
                     RPEGLAAALRALHPLGFAGCNLTIPHKQQAMTIVDEVDAVAKRIGAISCVVVGPDGSL
                     AGTNNDWFGFIHNLRQEQPGWRADAGPVAVIGAGGGSRAVCYGLVQ"
     gene            4440..4697
                     /locus_tag="AC158Q_09400"
     CDS             4440..4697
                     /locus_tag="AC158Q_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340032.1"
                     /translation="MATLMKPARSMDWTKERIDALQTPEVKQLRANAERLNDPQIVER
                     CDAVLADRPRGGGPGSVKPPKSTTPRARKKKAAPETTDEAA"
     gene            4694..5035
                     /locus_tag="AC158Q_09405"
     CDS             4694..5035
                     /locus_tag="AC158Q_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340033.1"
                     /translation="MTIPVVERGRFMIGKYEVIAEPIPHSMHMLRYTVYVGKTRIGAT
                     VSMPSESDCRFLEKPPIVPPLKIFYLTSKPGRPKKSAKPPVPLERDQPGFREEIPSDV
                     SLDGLGKNGDR"
     gene            complement(5159..5365)
                     /locus_tag="AC158Q_09410"
     CDS             complement(5159..5365)
                     /locus_tag="AC158Q_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR3340034.1"
                     /translation="MATGTVKWFNDAKGFGFITPDGGGEDLFAHFSSINMAGFKSLKE
                     GQKVSFEVTTGNKGKTQASNIQAA"
     gene            5667..5822
                     /locus_tag="AC158Q_09415"
     CDS             5667..5822
                     /locus_tag="AC158Q_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340035.1"
                     /translation="MTDEVYIAEYRKLANEYSGNQTSMGDYLVAIQTLKERYLKGKSS
                     AALPVVP"
     gene            5954..6772
                     /locus_tag="AC158Q_09420"
     CDS             5954..6772
                     /locus_tag="AC158Q_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340036.1"
                     /translation="MFDAIYSRRDPLAAAPIRVAVVVSVFVHAALILELPRPRPTPPG
                     LPDPGETESRLTVRLAPPLIAPPATPPAAPPAPRVRPRPPIVARTPPAPVVSSPAPSP
                     SAAAPAPPVAAGDLSSYIEGRRLARAAPAAPPDGAADAPPAESDNARANRLAAANLAS
                     PRTLTFGFDPKKSGGVFQINHVAYDYAEFTFVGWNIDARRQTKQLIEVRKGGSSDIRL
                     AVVRRMIGIIRQYEPEEFLWDSQRLGRSLMLSSRQRDNAGLEDFMMKEFFEESR"
     gene            6769..7974
                     /locus_tag="AC158Q_09425"
     CDS             6769..7974
                     /locus_tag="AC158Q_09425"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008161743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HIR3340037.1"
                     /translation="MKSVANAFLLLGALSAAPGSLAADFNACVRTLRGEAMGLGITSQ
                     TFDQAMKGVEPDPGVLDAMDNQPEFTTPVWDYLASLVDEQRIADGKAGLGEWAAVLEG
                     VEKKFGVDRHIVVAVWGVETNYGKNTGKRPLVRSLATVSCFGGRQRYFRGELVAALRI
                     LQSGDVPPEALTGSWAGAFGQTQFMPSTFHRSAVDFDGDGRRDIVNSVPDALASSANY
                     LKNAGWTSGQPWGYEVRLPEQYNGPSGRRSRRALAEWERAGIRGVNGEALAGSGRAAL
                     LLPAGAGGPAFIVFRNFDAIFAYNASESYALAIAHLSDRLRGAGPFRTAWPTDDPGLS
                     RAERRELQELLIRQGHDIGAADGMIGARTRAAIEAFQASAGLPVDGRAGGRTLRALRA
                     KSDKARMTK"
     gene            8051..>8484
                     /locus_tag="AC158Q_09430"
     CDS             8051..>8484
                     /locus_tag="AC158Q_09430"
                     /inference="COORDINATES: protein motif:HMM:NF045289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR3340038.1"
                     /translation="MDTNFAALEQVRSTAIDLGMKFGPKLLVALIIMGVGLLAARWIA
                     GVTERALRRFEFEAPMLRLLVTSSRVLVLVLFAIMALQNLGVELVPLIAGLGVAGAGI
                     ALAMQGVLGNLFAGLSIILTRPFHVGEYISIVKEEGQVADVSL"
BASE COUNT         1538 a         2787 c         2736 g         1423 t
ORIGIN      
        1 ggcgagcctg cccatgccgc gcgtgcccat cacgatggac tcgcaccggt agcggcgcgc
       61 cgcgcccgcg atcgtggcgg caacgtcgcc gacgactacg tgcgtcctgc acgccacccg
      121 cggcgagcgg acgcgcgtcg ccgcgcgttt cagaatggcc ttggcgcgtt cccgcatcag
      181 ccggctgtgc tgctgccgcg acagatgggc gcgcaccatg ccgtaatcat cgagcggcgg
      241 ctcgacattc agcaggtgca gggtaaccgg gcagtaccgg ctttgcgaga ccgcgtagtc
      301 cagcgcgcga aacgagttct ccgatccatc gacaggcacc agcagcgtct tcatgtcacc
      361 tccgttcgac tgagggtgcg atgctaacat ccggatagtt gcaggtcttg atcgagatca
      421 agccccgttg gcgcggcgcg cgacgggccg cgcaggccgc ggaacgaccc gcgggccccc
      481 ggtcccgggt cagccgttac ccgtcaccta ttcggtgcga ttccgctatc atccgcattt
      541 cgcaagtcaa cccagggagc ggtccatgtc gttgctgaac ctgtccatcg cgaccacgga
      601 ctacgatcat ttccgcgatt tccggctcgg ttacgtgaaa gccgaaggga tcgatctcaa
      661 ctggctcaat ctcggccacc acgaatgctt cgcgcgcttc acggccaatc gcgaattcga
      721 cctgtccgag ctttcgctcg cgaaattcgc cgcccaggtg acgcgcgagg gagccgacat
      781 catcgggctg cccgtcgtct gctcgcggct gttccgcttc agttcgttct acgtgaacaa
      841 gaaaagccgg atcaggaccg tgaaggacct ggctggcagg aaggtcggct cgccggaatg
      901 ggcgcattcg gcggcggtat acatgcgcgg ctggatgcac aacgacatgg gcgtgaagct
      961 cgctgacgtg cactggtacc aggccggcgc gaactcgccg gggcgcgagg aaaagatcga
     1021 gctgaatctt ccccggggcg tgaagctgac gcgcgtcgcc gacaagtcgc tcagccagct
     1081 gctggccgcg ggcgagatcg actgcgcgat catcgcgcgc ccgccgacct gcttcctgga
     1141 agaccacccg gacgtggagc gcctctttcc gaattatcac gagatggagg aggaatacta
     1201 cgagcggacc cgggtgtggc cgatcatgca catcatcgcg atgagaaagt ccgtcctgga
     1261 ggaccacccc tgggtcgcgc gcaatctcta caacgcattc ctggagtcga agcggcgcag
     1321 catcgagcgc ctgctcgatc ccgcggtgtc gcgctacccg ctggcgtggc tgcccaccta
     1381 cgcccgcacg atgcgcgaga tgttcggcgg cgacccgttt ccctatggca tcgaggagaa
     1441 ccgcgcgacc tgggagcaga tggcgctcta cacctggcag cagggcatcg cgcaccgccc
     1501 gttcaagccc gaggagatgt ttccggaggg cctgatgacc agggttgtta tctgaaaccg
     1561 tgatgagtga tgggtgacga gtaatgagtg caactcccaa atccggcggg ggcgtcccgg
     1621 ccgcattgat ctcagacctg gcggcggcga gccgcatcct cgccgcgcag ggcgtcgtgg
     1681 acggcttcgg gcatgtctcg atgcgccacc cccaggcgca cgaccgctac ttcatgtcgc
     1741 gctcgatcgc gccggcgctg gtgacgcccg gcgacctcat cgagtacgac ctcgacagcg
     1801 atccctgcaa tgccaacggc cgcaacagtt tcctcgagcg cttcatccat ggcgagatct
     1861 acaaggcgcg gccggacgtg atgtcggtcg tgcacagcca ttccccgtcg gtgatcccgt
     1921 ttgggctcgt ggccgtgccg atgcaggcga tgttccacaa cgcggcgttt ctcgcagcag
     1981 gcgtgccggt gttcgacatc aagaagcagt tcggcgccac cgatctcctg gtcagcgacg
     2041 gcgcgaaggg cgtggcgctc gccgagtcgc tgggagaaaa agatatcgcg atgatgcgcg
     2101 cgcatggcag cgtgacctgc gggcccacgc tgcagacggc ggtgttccgc gcggtctaca
     2161 ccgaggtcaa cgcgcgcatc cagcactgga ccctcgcatt ggccggcggc aagccgctgg
     2221 ccgtgctcga cgtcgaggaa ggccggctcg ccgacgtcat caaccagacc gccggcatgc
     2281 gcgcctggga cctctggcgc acgcaggtgc gggcgcaggt cggctggtag cgcaccggcc
     2341 gggggatgag gaagccatgt cggaaaccga tgtgctgaag cagcagctgg tggactgcat
     2401 ccgcatgctg gagcggtcgg acatcatcga ttacaacggg cacgccagca tccgcgcggg
     2461 cgacaaccgc atgttcatca acgccggttc ctgccagagg agcctgctca cggccggcga
     2521 catttgcacg atcgacttcg acggcaacgt gatcgacggc aacggcaagc cgccgctgga
     2581 gttccacctg cacgccggca tctacaagtc gcgctccgac gtgaaggcgg tcgtgcacgc
     2641 gcaccccaag tggtcaacgt tcctcaccat ggtcggggag agttaccagc cggtgtacgc
     2701 gcagggctcg ctggtctggc cgatgccggt gctcgattcg cccaactcga tcaacaaccc
     2761 ggcgatggcg aagcgcctcg cggacacgct cggcgaccgg ccggcggcga tgatgaagtc
     2821 gcacggcgcg gtgaccgtcg gcaaggacat cgtggaagcc ttcgtgctcg ccaactacct
     2881 cgaggaaaac gcctaccggc agtacatggc gctgcagatc ggcaagccgt attcattcag
     2941 cgacaaggaa gtcgcgctgt gccgcgagaa gctctggacc gagagcctgt tcaggcgcac
     3001 ctgggatcat ttccgcgcga aactcgactg acggtgtaaa gcctcaccgc caaggacgct
     3061 aatccgcaaa ggaaagctta cgacggatcg cgaagaacgc ggagagcgca aaggaaaaac
     3121 gaaagcttta ccgcaaagga aataacaatg aaagcaacag caaaatctga agggcgccaa
     3181 ggaatcgcgc caagggcggc ccgcaagaaa cgcttccggt cattttggcg ccgcatccag
     3241 ggcatttttg gaagcccgaa ctccaacggc gtatcaaata actcccttgg cgttgccttc
     3301 gcgttcttgt gtgtggcttt tgatctttga aaatttattc ctttgcggcc tttgcggtaa
     3361 ggcttttctg ttccttcgcg gccttggcgt cttggcggta aagcttttaa agcccgtcaa
     3421 gaatcatcac ggatcgactt ctccatcaac gcccgcaacc cgggcgtgac ttcgacgtcg
     3481 attccgaacc acgccttcca cgccgggcgg ccctggtgca gcagcatgcc gaggccgttg
     3541 acggtacggt tgccgcgggc gcgcgccgcg gcgaggaacg gggtttccag cgggatgtag
     3601 atgatgtcgg cggcaagcgc ggatttcggc agcctgtcca gccggatatc gagcgcgggc
     3661 tggcccacca tgccctggct ggtggcgttg accgccatcg ccgcgccttc cagcgcgtcg
     3721 tggcgctcac cccagggcag cgccttgacc gggccgccga attctccggc gagcgcctgc
     3781 gcgcgcgcga acgtgcggtt gacgagccgg atctccttcg cgccttactg cacgaggccg
     3841 tagcagaccg cgcgcgaacc gccgccggcg ccgatgactg cgaccggccc ggcgtccgcg
     3901 cgccagcccg gttgctcctg ccggaggttg tggatgaagc cgaaccagtc gttgttggtg
     3961 ccggcgagcg agccgtcggg cccgaccacc acgcaactga tcgcgccgat gcgcttcgcc
     4021 acggcatcca cttcgtccac gatcgtcatg gcctgctgct tgtgcgggat cgtcaggttg
     4081 catcccgcga aacccagcgg gtgtagcgcg cgcagcgccg cagcgaggcc ttcgggccgg
     4141 atggccagcg gcacgtaggt tcctgccagg tgacgctggc ggaaccagta gttgtgcatc
     4201 agcggcgagc gcgaatgcat gacgggccag cccattacgc cggccaggag gaaaagatcg
     4261 ggatgcgcca tcaggccatt ctactgaacc cgctgccccc gggttcacgt ccgagttgca
     4321 caacgctctg ggattcaagg ggtttttgcg ctataatgcg ccaccagtaa gcccgctccc
     4381 gccgggcttt tttgtttcgg caacgcctgt cggattcatt ccggaaagcg aaatcaacca
     4441 tggcaaccct catgaaaccg gcccggtcga tggactggac caaagaacga attgacgcgc
     4501 tgcaaacacc cgaggtgaag cagttgcgcg ccaacgcgga acggctcaac gacccgcaaa
     4561 tcgtggaacg ttgcgacgcc gtgctcgcgg accggccgcg cggcggcggt cccggcagcg
     4621 tcaagccccc caagagcacc acgccccggg cgcgcaagaa gaaagcggcg ccggaaacga
     4681 cggacgaggc cgcatgacga tacctgtcgt tgagcgcggg cggttcatga tcggcaagta
     4741 cgaagtgatc gcggagccga tcccgcattc gatgcacatg ctgcgctaca ccgtgtacgt
     4801 cggcaagacc cggatcgggg ccacggtcag catgccgtcc gaatccgatt gccgcttcct
     4861 ggagaagccg ccgatcgtgc ccccgctcaa gatcttttat ctcaccagca agcccggccg
     4921 cccgaagaaa agcgccaagc cccccgtacc cctggaacgg gaccagcccg gcttccggga
     4981 agagatcccc tccgacgtgt cgctcgacgg gctcgggaaa aacggggacc gctgaaaatt
     5041 ggcggggaat gaagcgacga attgatgcgg tgacgggtca cttcttcctt cggctaaccc
     5101 ggcgggtacg taaaaaaagc cgggcataag cccggctttt tcacgcagtg cggaccgctc
     5161 aggcggcctg gatgttgctg gcctgcgtct tgcccttgtt gccggtggtc acttcgaagg
     5221 acactttctg gccttccttc agcgacttga aacccgccat gttgattgac gagaagtggg
     5281 cgaagagatc ctcgccaccg ccgtcaggag taataaagcc gaaacccttc gcatcgttga
     5341 accacttgac tgtaccggtt gccattgtca cgttccttaa taaaaattag ggctgagccc
     5401 actgaatcgt gtttgatctc aaagccccac aagaacggac caacagcaag tcactatccc
     5461 acaagcactc ggactaaaca tcgagcgcgc accttatagg ggtgcagggt ctgcgtcaag
     5521 gaaaactccg aatcggggcc gaaaacgggt gatttttcgg caaaaaccgc gtcttttcac
     5581 cgaaagcagg aggaaatgcg ggttgcggcc ggttatccgc ccgctggcgc gtatagtgcc
     5641 ccatgcgtcg cgagacacga agcgggatga cagacgaagt ctacattgcc gaataccgga
     5701 agcttgcaaa cgagtactcc ggcaatcaga cgagcatggg cgactacctc gtcgccattc
     5761 aaacactgaa agaacgctac ctcaagggca agagcagcgc ggcgcttccc gtcgtcccgt
     5821 agcgcccccg ccggcgcgaa tgcccggcgt gtctgggcca cttccgcctt tcgcctgtcg
     5881 actttcgact ttcgctttgg ggtccccgcc ctcctcggat aggatttgcg tgtctgttgt
     5941 ctgataaatc atcgtgttcg acgccatcta cagccggcgt gatcccctgg cagcggcccc
     6001 catccgggtg gcggtcgtgg tgtccgtgtt cgtccatgcc gcgttgattc tggaattgcc
     6061 gcgaccgcgc ccaaccccgc ccggcctgcc cgatcccggc gaaacggaga gccggttgac
     6121 ggtgcggctg gcgcctccgc tcatcgcccc tccggcaacc ccgcccgccg cccctcccgc
     6181 gccccgggtg cggccgcggc caccgatcgt cgcgcgcaca ccaccggcgc cggtcgtctc
     6241 ctcgccagcg ccatcgcctt ccgccgctgc gccggcaccg cccgttgcgg ccggcgatct
     6301 ctcttcgtac atcgagggga gaaggcttgc ccgcgccgcg ccggcggcgc ctccggacgg
     6361 cgcggcggac gcacctccgg ccgaatccga caacgcccgc gccaaccgcc tcgccgccgc
     6421 caacctcgct tcgccgcgca cgctcacctt cggcttcgac ccgaagaaga gcggcggcgt
     6481 attccagatc aatcacgtgg cctacgacta tgccgagttc acgttcgtcg gctggaacat
     6541 cgacgcccgt cgccagacca agcagctgat cgaagtgcgg aaaggcggca gcagcgacat
     6601 ccgcctcgcc gtcgtgcgca ggatgatcgg catcatccgc cagtacgagc cggaggaatt
     6661 tctctgggac tcgcaacgcc tgggccgcag cctcatgctc tcctcgcgcc agcgcgacaa
     6721 cgccgggctg gaggatttca tgatgaagga atttttcgag gaaagccggt gaagtccgtg
     6781 gccaacgcgt tcctgctgct cggtgcactc tcggccgccc cgggttcgct cgccgccgat
     6841 ttcaacgcgt gcgtccggac gctgcgcggg gaggctatgg ggctgggcat cacctcgcag
     6901 accttcgacc aggccatgaa aggcgtcgag cccgacccgg gcgtgctgga cgccatggac
     6961 aatcagcccg agttcacgac gccggtctgg gattacctgg cctccctggt ggacgagcag
     7021 cgcatcgccg atggaaaagc gggtctcggc gaatgggccg ccgttctgga gggcgtggaa
     7081 aagaagttcg gcgtggaccg gcatatcgtg gtcgcggtat ggggcgtcga gacgaattac
     7141 ggaaaaaata cgggcaagcg accgctggtg cgctcgctcg cgacggtctc ttgcttcggc
     7201 ggccggcagc gctactttcg cggcgaactg gtcgcggcgc tgcgcatcct gcaaagcgga
     7261 gacgtgccgc ccgaggcgct gaccggctcc tgggccggcg cgttcggcca gacgcagttc
     7321 atgccctcga cgttccaccg ctcggcggtg gatttcgacg gcgacggccg ccgcgacatc
     7381 gtcaactcgg tacccgacgc gctcgcctcc tcggcaaact acctgaagaa cgcgggctgg
     7441 acgagcggcc agccgtgggg ctacgaggtg cgcctgccgg agcagtacaa cgggccgtcc
     7501 gggcgccgct cccggcgcgc gctggcggaa tgggagcggg cggggatccg gggcgtaaac
     7561 ggcgaggcgc ttgcgggcag tggccgggct gcgctgctgc tgcccgccgg cgcgggcggc
     7621 ccggcattca tcgtgttccg gaatttcgac gcgatcttcg cctataacgc ctccgaatcc
     7681 tatgcgctcg cgatcgccca cctgtcggac cgtctgcgcg gcgccggccc gttccggacc
     7741 gcgtggccca cggacgaccc gggcctgtcg cgcgccgagc ggcgagaact ccaggaactg
     7801 ctgatccggc agggccacga catcggcgca gccgacggca tgatcggggc gcgcacccgc
     7861 gcggcgatcg aggcgttcca ggcctcggcg gggctgccgg tggacggccg cgccggcggg
     7921 cgcaccttgc gcgccctgcg ggcaaaaagt gacaaggcgc ggatgacgaa gtagcgcggc
     7981 cggagttcaa gtcatctccc ggtccaggca gctagaatgt ccatgccgga tataaccgaa
     8041 aggatattcc atggacacca acttcgcggc actggaacag gtcagaagca cggccatcga
     8101 cctgggcatg aaattcgggc cgaaactgct ggtcgcgctg atcataatgg gagtcggcct
     8161 tctcgcggcg cgctggatcg ccggggtgac ggagcgcgcg ctcaggcggt tcgaattcga
     8221 ggcgccgatg ctccggctcc tggtgacaag ctcgcgcgtg ctggtgctgg tcctcttcgc
     8281 gatcatggcg ctgcagaacc tgggcgtgga gctggttcct cttatcgcgg ggctgggggt
     8341 ggcgggcgcg ggtatcgccc tggcgatgca aggcgtgctc ggcaacctgt tcgccggcct
     8401 gtccatcatc ctgacgcggc cgttccacgt cggggagtac atctcgatcg tcaaggaaga
     8461 aggccaggtg gccgacgtca gcct
//
LOCUS       DBNGSG010000363         7468 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1986814, whole genome shotgun sequence.
ACCESSION   DBNGSG010000363 DBNGSG010000000
VERSION     DBNGSG010000363.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7468)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7468)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7468
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1986814"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="AC158Q_09435"
     CDS             <1..191
                     /locus_tag="AC158Q_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3340039.1"
                     /translation="EPYVTTKPKGTGLGLAIVKRIVEDHGGSAAIANVAPRGARVTVR
                     LPAAAQDIARRAAARAPV"
     gene            215..1000
                     /locus_tag="AC158Q_09440"
                     /pseudo
     CDS             215..1000
                     /locus_tag="AC158Q_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743627.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
     gene            1016..1486
                     /locus_tag="AC158Q_09445"
     CDS             1016..1486
                     /locus_tag="AC158Q_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA-type ATPase lid domain-containing protein"
                     /protein_id="HIR3340040.1"
                     /translation="MPALREHREDVPDIANLTLSRMVEAKETPPRQFTTAALNALRNY
                     DWPGNLAQLESLVRTLALTCPGEQIPLEEVSRALPLPVAVMPVTHNLALDLPLRDARD
                     AFERAYFEHHIAREGGNISRVAEAVGLERTHLYRKLKQLGIRLSRKSDSEDQQQ"
     gene            1568..3568
                     /gene="tkt"
                     /locus_tag="AC158Q_09450"
     CDS             1568..3568
                     /gene="tkt"
                     /locus_tag="AC158Q_09450"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIR3340041.1"
                     /translation="MTARVELANAIRALAMDAVQQANSGHPGMPMGMAEIADVLWNDH
                     LRHNPANPAWPNRDRFVLSNGHGSMLLYAVLHLTGYDLPLEELKRFRQLNSKTPGHPE
                     YGCAPGVETTTGPLGQGIANAVGMALAERLLAAEFNRPELEIVDHHTYVFLGDGCMME
                     GVSHEACSLAGTLALGKLIAIYDDNGISIDSVKGNIRQWYTDDVKRRFEGYGWQVIAN
                     VDGHDVEAVNRAVKKAKREKSRPTLICAKTVIAKGAPTKANTGAAHGAPLGEKEVAAT
                     REAIGWNHPPFDIPKPVYEGWDAKKRGAAAERRWKKLFSAYEAQHPREAADFRRRTAG
                     ELPPDFGARAEALVAEAVSKAETVATRKASQSVLEALVPALPELIGGSADLAGSNLTL
                     VKASKAVGREGGGNYVFYGVREFGMCAVMNGLALHGGFAPYGGTFLVFSDYARNALRL
                     AALMKQRVIYVFTHDSIGLGEDGPTHQPVEHAASLRLIPGMDVWRPCDTVETAVAWVC
                     ALERANGTTSLLLTRQNLPYVQRDAQQLAAVKRGGYVISEAAGGAARAIVIATGSEVG
                     IALEAQKRLAQENVPVRVVSMPSTSLFDRQDEPYRASVLARGVPRVAVEAGVSEFWRK
                     YVGLEGAVLGIDRFGESAPGGELFRHFGFTPEKVVAAVKAAL"
     gene            3603..4604
                     /gene="gap"
                     /locus_tag="AC158Q_09455"
     CDS             3603..4604
                     /gene="gap"
                     /locus_tag="AC158Q_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIR3340042.1"
                     /translation="MTIKVGINGFGRIGRMVFRAAVQHFPDIEVVGINDLLEPDYLAY
                     MLRHDSVHGRFKGEVGVDGNTLIVNGRRIRLSQVKDPAELKWNEVGAEVVVESTGLFL
                     DKASGQKHLAAGASKVVMSAPSKDDTPMFVFGVNDRTYAGQAIISNASCTTNCLAPVA
                     KVLNDKWGIKRGLMTTVHAATATQKTVDGPSGKDWRGGRGILENIIPSSTGAAKAVGV
                     VIPELNKKLTGMAFRVPTSDVSVVDLTVELGKPATYKEICAEMKAQSAGALKGVLGYT
                     EEKVVATDFRGESCTSVFDADAGIALDPTFVKVVAWYDNEWGYSCKVLEMVRVVAKK"
     gene            4601..5791
                     /locus_tag="AC158Q_09460"
     CDS             4601..5791
                     /locus_tag="AC158Q_09460"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003278977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HIR3340043.1"
                     /translation="MTFLRLVDLPLAGKRVFIRADLNVPQDDAGHITEDTRIRASIPG
                     IEHALKNGAAVMVTSHLGRPTEGKLAPEDSLAPVAKRLGEMLGRPVRLVQNWVDGGFE
                     VGPGEVVMLENCRVNKGEKKNDEALARKMAALCDVYVNDAFGTAHRAEATTHGMAKFA
                     PVACAGPLMAGELDALGRALNHPARPLVAIVAGAKVSTKLTILKSLAAKVDQLIVGGG
                     IANTFMLAAGMPIGKSLAEAELAGAARDVMDIMKARGAQVPLPVDVVVANDLSVLARA
                     NRVAAAEVRPDDKILDIGPQTAATLAALIAKAGTVVWNGPVGVFEYDQFAGGTKTLAH
                     AIADSKAFSIAGGGDTLAAIAKFGITDRMSYISTGGGAFLEFLEGKKLPAVEILEQRA
                     PKQD"
     gene            5880..7307
                     /gene="pyk"
                     /locus_tag="AC158Q_09465"
     CDS             5880..7307
                     /gene="pyk"
                     /locus_tag="AC158Q_09465"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006406491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HIR3340044.1"
                     /translation="MPRRTKIVATLGPASSDAKTLARMIEAGLDVVRMNFSHGTADEH
                     RRRVELVRSLARKAGRAVGVLVDLQGPKIRIGRFRDGKVTLAAGAKFVLDAECELGDQ
                     HTVGLDYKKLPNDVRTGDTLLLDDGRIVLGVASVRGSRITCVVEQGGPLSNNKGINRK
                     GGGLTAPALTEKDKQDIRTAAALKADFLGVSFPRSGEDMRWARDLMHKAGGRALTVAK
                     IERAEAIPALEDILDASDCIMVARGDLAVEVGDAVVPALQKRMIRMAREKNRIVITAT
                     QMMESMIENPIPTRAEVSDVANAVLDGTDAVMLSAETAAGKYPVETVAAMARVCVEAE
                     KEDTLSQERQRRGPLEEVEEAIARATVFTANNLDIKAVASLTQSGKTVLLMSRMHTAV
                     PIYAMSSVIETRRRVTLFRGVYPVKFKGARNPEKALHMAEDELLKRGAVQNGDYIVLT
                     IGEPFGKAGGTNTMKVVKVGEHRKA"
BASE COUNT         1339 a         2453 c         2545 g         1131 t
ORIGIN      
        1 tcgagccgta cgtcacgacc aagcccaagg gcaccggcct cgggctcgcg atcgtcaaga
       61 ggatcgtcga agaccacggc ggctcggcgg cgattgccaa cgtcgcgccg cgcggcgcgc
      121 gcgtcaccgt tcgcctgccg gcggcggcgc aggatatcgc gcgccgcgcc gcggcgcgcg
      181 ccccggtata aactaacggc ctcattcccc gcttatgcag caaatcctgg tcgtagacga
      241 cgaaatcggc atccgcgagc tgctctccga gatcctctcc gacgagggct atcaggtgcg
      301 gctcgccgag aacgcgagtt cggcgcgcgc gtgccgcagc cagtcgcgcc ccgacctggt
      361 gctgctcgat atctggatgc ccgataccga cggcatcacg ctgctcaagg aatgggcggc
      421 cgcggggctg ctcaccatgc cggtggtgat gatgtcgggc cacggcacca tcgacacggc
      481 ggtcgaggcg acccgcatcg gcgcttacga ttttcttgag aagccgatcg cgctgcaaaa
      541 gctgctctcc accgtcgggc gcgcgctgaa gcaggggggc gagcaggcgc agccggcgct
      601 gtcgctggcg agcctggggc gcgcgccgct ggtgatcgag ctgaaacagc gcctcgagcg
      661 cgtcatgaac ctgcgcacgc ccctgctgct aaccggagag ccgggttgcg gactggagat
      721 cgcggcgcga ttcctgcacc agcgcaacac gccgtgggtc gcgccggaaa atcacgggtc
      781 gctggccgag gcgccgctcg acctgctcgg ccaggcgcgc gagggcacgc tgttcctgcg
      841 cgaggtctcg gaactgtccc gcatcgagca gaaagggctg ctgctcgccg cctccaagct
      901 cgaacgctac aacgtgcggc tcgtgagcgg cgcgagttgc gacctggcgg agctggttgc
      961 gcagggcacg ttcgacagcc ggctctacag cacgctggcc gccgccacga tccgggtgcc
     1021 tgcactgcgc gagcaccgcg aggatgttcc cgacatcgcc aatctgacgc tctcgcgcat
     1081 ggtggaggcg aaggagacgc cgccgcgcca gttcaccacc gcggcgctga acgccctgcg
     1141 gaattacgac tggccaggca acctcgcgca gctcgaaagc ctggtgcgca cgctcgcgct
     1201 gacctgcccg ggtgagcaga tcccgctgga agaagtcagc cgcgcgctgc cgctgccggt
     1261 cgcggtgatg ccggtcacgc acaacctggc cctcgacctg ccgctgcgcg acgcccgcga
     1321 tgccttcgag cgcgcttatt tcgagcatca catcgcgcgc gaggggggca atatctcgcg
     1381 cgtggcggaa gccgtcgggc tcgagcgcac ccatttgtac cgcaagttga agcagctcgg
     1441 tatccgcctc tcccgcaaga gcgacagcga ggaccaacag cagtaggaaa ttgcgcgctc
     1501 gcgccgctgc ctgccgcgcc cgtttataat gggcgcactg tttgccgcct cgcaaggaaa
     1561 atcccgaatg actgcccgtg tcgagctggc caacgcgatt cgcgcgctgg cgatggacgc
     1621 cgtacagcag gcgaactccg gacaccccgg gatgcccatg ggcatggcgg aaatcgcgga
     1681 cgtgctgtgg aacgatcacc tgcgccacaa ccccgccaat cccgcctggc ccaaccgcga
     1741 ccgcttcgtg ctgtccaacg ggcacggctc gatgctgctc tacgccgtgc tgcacctgac
     1801 cggttacgac ctcccgctcg aggagttgaa gcgcttccgg cagctcaatt cgaaaacgcc
     1861 cggtcacccc gagtacggct gcgccccggg cgtcgagacc accaccggcc cgctcggcca
     1921 gggcatcgcc aacgccgtcg gcatggcgct ggcggagcgg ctgctggccg cggaattcaa
     1981 ccggcccgag ctggaaatcg tcgatcacca tacgtacgtt ttcctgggcg acggttgcat
     2041 gatggaaggc gtctcgcacg aggcgtgctc gcttgccggc acgctcgcgc tgggcaaatt
     2101 gatcgcgatc tacgacgaca acggcatctc gatcgattcc gtgaaaggca acatccgcca
     2161 gtggtacacg gatgacgtga aacggcgctt cgaaggctat ggctggcagg tgattgcgaa
     2221 cgtggacggc cacgacgtgg aagcggtcaa ccgggcggta aagaaagcga agcgcgagaa
     2281 atcgcgcccg acgctgattt gcgcgaagac cgtgatcgcc aagggcgccc ccaccaaggc
     2341 caacaccggc gccgcgcacg gcgcgccgct gggcgagaag gaagttgccg ccacgcgcga
     2401 ggcgatcggg tggaaccatc cgcccttcga catcccgaaa cccgtgtacg aaggctggga
     2461 cgcgaagaag cgcggcgccg ccgccgagcg gcgctggaag aagcttttct ccgcatacga
     2521 ggcgcagcat ccgcgcgagg cggccgattt ccgccgccgc acggcgggcg agctgccgcc
     2581 ggatttcggc gcccgcgccg aggcgctcgt ggcggaagcc gtgtcaaagg ccgagaccgt
     2641 ggcaacgcgc aaggcctcgc aaagcgtgct cgaggcactg gtgccggcgc tgcccgagct
     2701 gatcggcggc tcggccgatc tcgccggctc caacctcacc ctggtgaagg catcgaaggc
     2761 cgtcggccgc gaaggcgggg gcaattatgt tttctacggc gtgcgcgaat tcggcatgtg
     2821 cgcggtgatg aacgggctgg cgctgcacgg cggcttcgcc ccgtacggcg gcacctttct
     2881 ggtgttctcc gactacgcgc ggaacgcgct gcgccttgcc gcgctgatga agcagcgcgt
     2941 gatctacgtg ttcacgcacg attcgatcgg tctgggcgag gacgggccga cgcaccagcc
     3001 ggtcgagcac gcggccagcc tcaggctgat tcccggcatg gacgtgtggc ggccttgcga
     3061 caccgtcgag accgcggtgg cctgggtctg cgcgctcgaa cgcgcaaacg gcaccaccag
     3121 cctgctgctt acccgccaga acctgcctta cgtgcaacgc gacgcccagc agctcgccgc
     3181 ggtgaagcgc ggcggctacg tgatctcgga agcggcgggc ggcgcggcgc gcgcgatcgt
     3241 gatcgcgacc ggctccgagg tcggcatcgc gctcgaagcg caaaagcggc tcgcgcagga
     3301 aaacgtgccg gtgcgcgtcg tgtccatgcc gtcgaccagc ctattcgacc gccaggacga
     3361 gccatatcgc gccagcgtgc ttgcgcgcgg cgtgccgcgc gtggcggtgg aggcaggcgt
     3421 gtccgaattc tggcgcaagt acgtgggact ggagggcgcc gtgctgggca tcgaccgctt
     3481 cggcgagtcg gcgccgggtg gagaactatt caggcatttt ggtttcacac cggaaaaggt
     3541 cgtggccgcc gtcaaggcgg cactgtgaaa gcggggttca acgtgaattt ctggagaacg
     3601 gcatgactat caaggtcggc atcaacggat tcggccgcat cgggcgcatg gtatttcggg
     3661 cggcggtcca gcatttcccg gacatcgagg tggtcggcat caacgacctg ttggagccgg
     3721 actacctcgc ctatatgctg cggcacgatt ccgtccacgg ccgcttcaag ggcgaggtgg
     3781 gcgtcgacgg caacacgctg atcgtcaacg gccgcagaat ccggctctcc caggtcaagg
     3841 acccggccga gttgaagtgg aacgaggtgg gggcggaggt cgtggtggaa tccaccggcc
     3901 tgttcctcga caaggcgtcg ggccagaagc acctcgctgc gggcgcaagc aaagtcgtca
     3961 tgtcggcgcc gtcgaaggac gacacgccga tgttcgtctt cggggtgaac gacaggacct
     4021 acgccgggca ggcgatcatc tccaacgcgt cgtgcaccac caactgcctc gccccggtgg
     4081 cgaaggtgct gaacgacaag tggggcatca agcgcggcct gatgaccacg gtgcatgccg
     4141 ccaccgccac gcagaaaacc gtcgacgggc cttccggcaa ggactggcgc ggcgggcgcg
     4201 gcatactcga gaacatcatt ccctcctcca ccggggccgc aaaggcggtg ggcgtggtga
     4261 ttccggagct gaacaagaag ctgaccggca tggcgttccg cgtgccgacc tcggatgtct
     4321 ccgtcgtcga tctcaccgtc gagctcggca agcccgccac ctataaggaa atctgcgccg
     4381 agatgaaagc gcagtccgcg ggcgccctga aaggcgtgtt gggctatacc gaggagaaag
     4441 tcgtcgccac cgatttccgc ggcgagagct gcacgtcggt gttcgacgcg gatgccggca
     4501 tcgcgctcga tcccaccttc gtcaaggtcg tcgcgtggta cgacaacgag tgggggtatt
     4561 cgtgcaaggt gctggagatg gtccgggtcg tggcgaagaa atgacgtttc tgcgcctggt
     4621 tgacctgcct ctcgcaggca agcgcgtctt catccgcgcc gacctgaacg ttccccagga
     4681 cgacgcgggc cacatcacgg aagacacgcg tatccgcgct tccatccccg gcatcgagca
     4741 cgcgctgaag aacggcgccg ccgtgatggt gacctcgcat ctcggacggc ccaccgaggg
     4801 caagctcgcg cccgaggatt cccttgcgcc ggtcgcgaaa cgcctggggg agatgctcgg
     4861 ccgcccggtg cggctggtgc agaactgggt ggacggcggc ttcgaggtgg gtccgggcga
     4921 agtggtgatg ctggagaact gccgcgtcaa caagggagag aagaagaacg acgaggcgct
     4981 ggcccgcaaa atggccgccc tgtgcgacgt ctacgtgaac gacgctttcg gcaccgcgca
     5041 ccgcgctgaa gcgaccacgc acggcatggc gaaattcgcc ccggttgcct gcgccgggcc
     5101 gctgatggcg ggcgagctcg acgcgctggg acgcgcgctc aaccatcccg cgcgcccgct
     5161 ggtggcgatc gtcgccggcg ccaaggtctc gaccaagctc accatcctga aatcacttgc
     5221 cgcgaaagtg gaccagctga tcgtgggcgg cggcatcgcc aatactttca tgctcgcggc
     5281 aggcatgccc atcggaaaat cgctcgccga ggccgagctc gcaggcgcgg cacgggacgt
     5341 catggacatc atgaaagcgc gcggcgcgca ggtgccgctg ccggtggacg tggtggtggc
     5401 caacgacctc tccgttctgg cccgcgccaa ccgcgtcgcc gctgccgagg tccggcccga
     5461 tgacaagatt ctcgatatcg gtccgcagac ggccgcgacg ctggcggcgt tgatcgccaa
     5521 ggccggcacc gtcgtctgga acgggccggt cggcgtgttc gagtacgacc agtttgccgg
     5581 gggcacgaag accctcgccc acgccatagc cgactccaag gcgttttcca tcgccggcgg
     5641 cggtgacacg ctggccgcga tagcgaagtt cgggatcacc gaccggatgt cctacatctc
     5701 gaccggcggc ggcgcgttcc tcgagtttct cgaaggcaag aagctgcccg cggtcgaaat
     5761 tctcgagcag cgcgccccaa agcaggattg aggatcgcgg gatgagattt gagtttgtgg
     5821 tggcgggaca ctacactgtc ctcaatcctc cttccggatc gctcgatccc ggctttcgca
     5881 tgccccgccg caccaagatc gtcgccaccc tcggacccgc gtcgagcgat gccaaaacgc
     5941 tggcgcgcat gatcgaagcc ggcctcgacg tggtgcgcat gaatttctcc cacggcacgg
     6001 ccgacgagca tcgcaggcgc gtcgagctgg tgcgctcgct cgcgcgcaag gcggggcgcg
     6061 ccgtcggcgt gctggtggac ctgcagggcc ccaagatccg catcggccgc ttcagggacg
     6121 gcaaggtcac gctcgccgcc ggcgcgaagt tcgtgctcga cgccgaatgc gagctgggcg
     6181 accagcacac ggtcgggctc gactacaaga agctgccgaa cgacgtgcgc accggcgaca
     6241 cgctgctgct cgacgacggg cgcatcgtgc tgggcgtcgc ttccgtcagg ggctcgcgca
     6301 ttacctgcgt cgtggaacag ggcgggccgc tgtccaacaa caagggcatc aaccgcaagg
     6361 gcggcggcct gaccgctccc gcgctgaccg agaaggacaa gcaggacatc aggaccgccg
     6421 ccgcgctgaa ggccgatttc ctcggcgtct cgttcccgcg ctcgggcgag gacatgcgct
     6481 gggcgcgcga tctcatgcac aaggcgggcg ggcgggcgct gacggtcgcg aaaatcgagc
     6541 gcgcggaggc gattcccgcg ctggaggaca tcctcgatgc ctccgactgc atcatggtcg
     6601 cccgcggcga tctcgcggtg gaagtgggcg atgcggtggt gccggcgctg cagaaacgca
     6661 tgatccgcat ggcgcgcgag aagaaccgca tcgtcatcac cgccacgcag atgatggaat
     6721 cgatgatcga gaacccgatt ccgacgcgcg cggaggtctc ggacgtcgcg aacgcggtgc
     6781 tcgacggcac cgacgcggtc atgctctccg ccgagaccgc ggccgggaaa tatccggtgg
     6841 aaacggtggc ggcgatggcg cgggtctgcg tcgaggccga gaaggaagat acgctttcgc
     6901 aggagcggca acgccgcggc ccgctggagg aggtcgaaga agccatcgcg cgcgccaccg
     6961 tcttcacggc caataacctc gacatcaagg cggtggcgtc gctgacgcaa agcggcaaga
     7021 cggtgctgct gatgtcgcgc atgcatacgg ctgtgccgat atacgccatg agctcggtga
     7081 tcgagacgcg gcgccgggtc accctgttcc gcggggtcta tccggtaaaa ttcaagggcg
     7141 cgcgcaaccc ggaaaaggcg cttcatatgg cggaagacga gctgctgaag cgcggcgcgg
     7201 tccagaacgg agactatatc gtgctgacga tcggggaacc tttcggcaag gccgggggca
     7261 ctaataccat gaaggtcgtc aaggtcggtg agcaccggaa agcgtaagtc gtgaacggca
     7321 agtagcgata aaataccccc cggttccgtc agcctacgac ccgccattca cgcagttcga
     7381 ggagatatca tgtccactca atcacttgag accgtcgccc aggcgatggt cgcgcccggc
     7441 aagggcatac tcgccgccga cgaatcca
//
LOCUS       DBNGSG010000364         2487 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1306716, whole genome shotgun sequence.
ACCESSION   DBNGSG010000364 DBNGSG010000000
VERSION     DBNGSG010000364.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1306716"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..1247
                     /locus_tag="AC158Q_09470"
     CDS             51..1247
                     /locus_tag="AC158Q_09470"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3340045.1"
                     /translation="MSVEKCVLREVFHSTPDARPSNCGVDHYQVLEQAIMDAAEKSKK
                     DPRVEAAISHWAPRFVVNGVPLTDFQEVTAGCDCWEDWCAAWGARAAVHEGLGNQALK
                     EGFRLSAGEHYTRAALCYHFGKFLFVNDMPQMKQAHAKVVECGNKALPLLDPPGERVA
                     IPYQGRQLYGNLRKPEGSTKPPVVVMCMGLDSTKEEMHDYEERFLKRGVATLAFDGPG
                     QGEAEYDFAICPEYEKPVAAVIDFVETRKDLDGGRVGIWGVSFGGYYAPRAAAFDKRI
                     KACIALSGAYQSSASFEGRPVINAEAFRVRSHSKDLEEAGRVALRVSLKGIAKNITCP
                     IYIVAGTKDRLTPVEAAKQLAAEVSGPCVLSIIEGGNHVVNNLWYRYRDQTADWMAER
                     LGVPTR"
     gene            1361..>2487
                     /locus_tag="AC158Q_09475"
     CDS             1361..>2487
                     /locus_tag="AC158Q_09475"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013486.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR3340046.1"
                     /translation="MARKRKVEPKHVQASGIDPKRGAKAGPDGLYDPANPGHRWDRPL
                     QASGRMQVDFEERVDFRRLHEYRLARTRNALKKSGLGAMLVFDQYNIRYISSTVIGEW
                     ARDKLTRWCLLTGNGEPWVWDFGSAARHHKLYAPWLPKNHCLAGNVGLRGAINPKVGL
                     FEAAAKEIFDILKQEGVSKMALGLDVCEPRFLFALQKLGLKVVDCQQVMLDAREIKSH
                     DEITLLNIAAAMGDGVYQDIFEALKPGVRENEIVAMATKRFYEMGSDCVEAINAIAGE
                     RCSPHPHNFTDRLIRPGDQAYFDLIQTYMGYKTCYYRTFTVGSATEAQHDAYRKAREW
                     MDSAIDSLKPGVSTDRVVQAFPKAQEIGFESEMAAFGLNFC"
BASE COUNT          480 a          757 c          842 g          408 t
ORIGIN      
        1 cgcttgggtt tgcccaagcg ctgagagtgt cgaggaatga gtgtcgaggg atgagcgtcg
       61 agaaatgcgt gttgcgggag gtttttcact cgacacccga cgcccgaccc tcgaattgcg
      121 gcgttgatca ctaccaggta ttggagcaag cgataatgga tgccgccgag aagtcgaaaa
      181 aagatcccag ggtggaagcc gcaatttcgc actgggcgcc gcgctttgtc gtgaacggcg
      241 tgccgctgac ggatttccag gaggtgacgg ccggctgcga ctgctgggaa gactggtgcg
      301 cggcgtgggg cgcgcgtgcc gcggtccatg aggggctggg caaccaggcc ctgaaggagg
      361 gattcaggct ctccgccgga gagcactaca cgcgcgccgc gctgtgctac cacttcggaa
      421 agtttctgtt cgtgaacgac atgccgcaga tgaagcaggc gcacgcgaag gtggtggagt
      481 gcggcaacaa ggcgttgccg ctgctcgatc cgcccggcga gcgcgtcgcg attccgtacc
      541 agggcaggca actctacggc aacctccgga agcccgaagg ttcgacgaag ccgcccgtag
      601 tcgtgatgtg catgggcctt gactctacca aggaggagat gcacgactac gaggaacggt
      661 ttttgaagcg cggcgtggcg acgctcgcct tcgacgggcc ggggcagggc gaggccgagt
      721 acgatttcgc gatctgcccg gaatacgaga agccggtggc cgcggtcatc gacttcgtcg
      781 agacgcgcaa ggacctggac ggtgggcgcg tcggtatctg gggcgtgtcc ttcggcggct
      841 actacgcgcc gcgcgcggcc gcgtttgaca agcgcatcaa ggcctgcatc gccctctcgg
      901 gcgcctacca gagctcggcg agcttcgagg gccggccggt catcaacgcc gaggcgttcc
      961 gcgtgcgctc ccacagcaag gacctggagg aggcgggcag agtcgcgctg cgcgtctcgc
     1021 tgaagggcat cgcgaagaac atcacctgcc cgatctacat cgtcgcgggg acgaaggacc
     1081 gcttgacccc ggtggaagcg gcgaagcagc tcgcggcgga agtctcgggc ccctgcgtgc
     1141 tgtccatcat cgaaggtggc aaccacgtcg tgaataatct ctggtaccgc tatcgcgacc
     1201 agaccgcgga ctggatggcc gagcgcctgg gcgtgcccac gcgctagtgc tgcgccatga
     1261 gttacaagtt acaagtctca agtttgaaac gccttgcgac ctgtatcctg caacttgtaa
     1321 cttgaaactt gtgactggca attgatagaa agagtcgcat atggcccgaa agagaaaagt
     1381 tgaaccgaag catgtgcagg cgtcgggcat cgacccgaag cgcggggcga aagcggggcc
     1441 ggatggtctc tacgaccccg cgaatcccgg ccaccgctgg gaccggccgc tgcaagcgtc
     1501 cgggcgcatg caggtggatt tcgaagagcg cgtggatttc cgccggctgc acgagtatcg
     1561 ccttgcgcgc acgcgcaatg cgctgaaaaa atcgggcctg ggcgcgatgc tggtgttcga
     1621 ccagtacaac atccgctaca tctccagcac cgtcatcggc gagtgggcgc gcgacaagct
     1681 cacgcgctgg tgcctgctga ccggaaacgg cgagccctgg gtgtgggatt tcggctccgc
     1741 cgcgcgccac cacaagctct acgcgccgtg gctgccgaag aaccactgcc tcgccggcaa
     1801 cgtcggcctg cgcggcgcca tcaaccccaa ggtcgggctg ttcgaggctg cggcgaagga
     1861 gattttcgac atcctcaagc aggaaggggt gtcgaagatg gcgctcgggc tggacgtctg
     1921 cgagccgcgc ttcctgtttg cgctgcagaa gctggggctg aaagtcgtgg actgccagca
     1981 ggtgatgctg gacgcgcgcg agatcaagag ccacgacgag atcacgctgc tcaacatcgc
     2041 cgcggcgatg ggcgacggcg tctaccagga catcttcgag gcgctgaagc ccggggtgcg
     2101 cgagaacgag atcgtcgcga tggcgaccaa gcgcttctac gagatgggct cggattgcgt
     2161 ggaggccatc aacgcgatcg ccggcgagcg ctgcagcccg cacccgcaca acttcaccga
     2221 ccgcctgatc cgccccggcg accaggccta cttcgatctc atccagacct acatgggcta
     2281 caagacgtgc tactaccgca ccttcaccgt gggctcggcg accgaggcgc agcacgacgc
     2341 ctaccgcaag gcgcgcgaat ggatggacag cgcgatcgat tccctcaagc cgggcgtcag
     2401 caccgaccgc gtcgtgcagg ccttccccaa ggcgcaggaa atcggtttcg agtccgagat
     2461 ggcggcgttc ggactgaact tctgcca
//
LOCUS       DBNGSG010000365         5659 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1646773, whole genome shotgun sequence.
ACCESSION   DBNGSG010000365 DBNGSG010000000
VERSION     DBNGSG010000365.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5659)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5659)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5659
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1646773"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="AC158Q_09480"
     CDS             complement(<1..393)
                     /locus_tag="AC158Q_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HIR3340047.1"
                     /translation="MKRSEKGNPLDLRAWLDEARKLGEVTDVRGADWNLELGAISELN
                     VKKDAPPALLFDEIAGYPKGHRVLTCSTSSPGKLSSILRLGAEKSHHGLVQKLRGLPK
                     TWQAEAVNYDPVELASGPALENVRSDGQI"
     gene            complement(581..1060)
                     /locus_tag="AC158Q_09485"
     CDS             complement(581..1060)
                     /locus_tag="AC158Q_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007744061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3340048.1"
                     /translation="MSAIQRTIRFTLNGEQVSAEVPAHWNVVELLQNRFALFGARESC
                     GPGLCGCCTVLVNGTAVSGCLYLAAFVDGAEVRTIEQPAPGTGLDAVQEAFIESGAFQ
                     CGFCTPGFVLMTRQLLDANPDPSDDEIRHYLSGNLCRCAAYPEIVAAVKLAAKKRKK"
     gene            complement(1184..3460)
                     /locus_tag="AC158Q_09490"
     CDS             complement(1184..3460)
                     /locus_tag="AC158Q_09490"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007744060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3340049.1"
                     /translation="MAKTAQSGPVGRSIPRLEGRAKVTGRAEYVHNLRLPGMLHGKIF
                     RSTVAHGRIKRIDTGPARAVEGVHRVITGADIRAIIPDPHYGPAFHDQPILALDKVRY
                     AGEPVALVLADDPHVAEEAAGRIAAEYEELPAVYDEVEAMTSKAIVHEVLKPAGTFPD
                     LKHLQGRKNTNIALDYHLRRGDAEAAFRKADRVFEHTFRTQQVLHVPLEPFVSAAVPG
                     DGTITIHTASQSPSFVRIEIARLLGWPENKVRVKVPYLGGGFGAKLYIKLEALVAAAA
                     LLTGRPVKVSLTMEEQFFTLTKHAATLRIKSGVTKNGRITGRLCEVWWNGGAYADIGP
                     RITQKSGFTAPGPYDIENVLIDSYALYTNLPAAGALRGFGIPQLVWAYESHTDMVARE
                     LKLDPVEFRRRNILRDGRPQATGTVMKDAAIGKVLERTAARMNWGKPFDRGNGTIRRG
                     RGIAIGFKAVVAPTTSVAIVNLYADGSCALYCSTVDMGQGSDTAMAQIVAEVLGIPIE
                     SITVVHPDTDVTPYDMATLGSRSTFHMGHAVRRASEDARDKLIALAQEVGLSVETTPT
                     RDVFRKKFGMQAGNIVGSGTYVPASAPTDHETGQCDNVTPYWMIGASGAEVEVDTETG
                     RVTVTRLVNVADVGRPINPDIVETQLSGAAVMQLGFTMSEKMEFSAGQCTNPSLADYK
                     IPGLLDIPPVMENEVVDAEQGSGPFGAKGVGESGTFGVSPAIANAIEDAVGVRITTLP
                     ITAESVYRALREKQGKPL"
     gene            complement(3518..4378)
                     /locus_tag="AC158Q_09495"
     CDS             complement(3518..4378)
                     /locus_tag="AC158Q_09495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR3340050.1"
                     /translation="MTPFELLEPRSLREAAALLGSGEPSARPVAGGTAVMLMMKLGVL
                     RPARLVSLRAVEPRYADIAIGPDGELRIGALVTLAALERSPVIHKEAPVIARTLNTLA
                     NVRVRNVATVGGHLAHGDPHMDLPPVLIALGARAVIVGAQGERTMPLEKLHAGYLETT
                     LGRGELIAEVIVPRQGARRAAYLKCTTRSADDWPALGVAVVLDMDGAAIRDAHIVISA
                     ATDTPTRLIKAESVLRGAGADEALLKRAGEAAAAESGVIGDAHGSAAYKRELVRVYLA
                     RAVRAALAGQ"
     gene            4484..>5659
                     /locus_tag="AC158Q_09500"
     CDS             4484..>5659
                     /locus_tag="AC158Q_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR3340051.1"
                     /translation="MRAIRNIAIIAHVDHGKTTLVDKLLRQSGTFAAHQHLVERVMDS
                     NDLERERGITILAKNCAVTYAGTHINIVDTPGHADFGGEVERVLTMVDGVLLLVDAVD
                     GPMPQTRFVTRKALAQGLKPVVVINKIDRPGARPDWVVNHTFDLFDKLGATEAQLDFP
                     IVYTSALNGTATRDLAKPGTDMRPLFEAILEQVPVRADDPDGPLQLQICSLDYSSYVG
                     RIGIGRVNRGRIRSGQQVAVLHGPDSTPVIAKVNQVLVFKGLERVLAEEAQAGDIVLV
                     NGIEEVGVGVTLADPARPEALPLLKVDEPTLVMNFQVNTSPFAGREGKFVTSRQLRER
                     LAREVMSNVALRVEDTEDPDVFKVSGRGELHLTILLENMRREGYELAVSRPRVLLKEI
                     "
BASE COUNT          940 a         1888 c         1811 g         1020 t
ORIGIN      
        1 gatctggccg tcgctcctca cgttctcgag ggccggcccg ctggcgagct ccactggatc
       61 gtagttgacc gcctccgcct gccaggtctt cggcagcccg cgcagcttct gcaccaggcc
      121 gtggtgcgac ttctccgcgc cgaggcgcag gatcgaagaa agcttcccgg gactgctggt
      181 gctgcaggtc aggacgcggt ggcccttcgg gtagcccgcg atctcgtcga acaggagcgc
      241 cggcggcgcg tccttcttga cgttcagctc gctgatcgcg ccgagctcga ggttccagtc
      301 ggcgccgcgc acgtccgtca cctcgccgag cttgcgcgcc tcgtcgagcc aggcgcggag
      361 gtcgagcggg ttgccttttt ctgatcgctt cacgtcaaga cctcgaacgg acaggattca
      421 tttttcaaac acccgttaaa agcgacaata gacaggatta acacgattaa caagattcaa
      481 gcaaaagatt ctatgaaacc aagtattaat tctgtaaatc ctgttaatcc tgtccattta
      541 ggggcttgac gggttttcga tcgaagcttg ttaatcctgt ctatttcttt cgcttcttcg
      601 cagcgagctt caccgccgcg acgatctccg gataggccgc gcagcggcac aggttccccg
      661 acaggtagtg ccggatctcg tcgtcggacg ggtcgggatt cgcatcgagc agctggcgcg
      721 tcatcagcac gaagcccggc gtgcagaacc cgcactggaa cgccccagat tcgatgaagg
      781 cttcctgcac ggcgtcgagc cccgtgccgg gcgccggctg ctcgatcgtc ctcacctccg
      841 cgccgtccac gaaggctgcg aggtagagac agcccgacac ggcagtcccg ttcaccagca
      901 ccgtgcagca cccgcacagc cctggcccgc agctctcacg cgcgccgaac agcgcgaaac
      961 ggttctgcag caattccacg acgttccaat gcgcgggaac ctccgccgag acttgctccc
     1021 cgttcagcgt gaagcgaatg gtgcgctgaa tggcgctcat cggcggcccc cgctgaggga
     1081 tgagggatgc gggatgaggg atgaggagct catttctgaa atcttggcgg cttggcggtt
     1141 caacattgtt gttgattctc ttggcgggct tggcggttca gccttacagg ggcttgcctt
     1201 gcttctcgcg cagtgcgcga tagaccgatt cggcggtgat cggcaaggtg gtgatgcgca
     1261 cgccgaccgc gtcctcgatt gcgttggcga tcgccggcga cacgccgaac gtgccggatt
     1321 cgccgacgcc tttcgcgccg aaaggcccgc tgccctgctc ggcgtccacg acctcgtttt
     1381 ccatgacggg ggggatatcg agcaggccgg gtatcttgta atcggcaagc gacgggttgg
     1441 tgcattgccc ggcgctgaat tccatcttct cggacatcgt gaatcccagt tgcatgaccg
     1501 ctgcgccgga gagctgggtc tcgacaatgt ccgggttgat cggccggccg acgtcggcga
     1561 cattgacgag ccgcgtcacg gtgacgcgcc cggtctcggt gtccacttcc acttccgcgc
     1621 cgctcgcccc gatcatccag tagggcgtca cgttgtcgca ctggccggtc tcgtgatcgg
     1681 tgggcgcgga ggccgggacg taggtgccgc tgccgacgat attgcccgcc tgcatcccga
     1741 attttttccg gaatacgtcg cgggtcgggg tcgtttcgac ggagaggccg acttcctgcg
     1801 cgagcgcgat cagcttgtcg cgcgcatcct cggacgcccg cctcaccgca tgccccatgt
     1861 ggaatgttga acgcgagcca agcgtcgcca tgtcgtaggg tgtcacgtcg gtgtcgggat
     1921 gcaccaccgt gatggattcg atcgggatgc cgagcacctc cgcaacgatc tgcgccatcg
     1981 cggtgtcgga gccctgcccc atgtccaccg tgctgcagta aagcgcgcag ctgccatcgg
     2041 cgtagagatt gacgatagcg accgatgtgg tgggcgcgac gaccgccttg aatccgatcg
     2101 cgatgccgcg cccgcgccgg atcgtgccgt tgcccctgtc gaaaggcttg ccccagttca
     2161 tccgcgccgc cgtgcgctcg agaaccttgc cgatggcggc gtccttcatg acggtgccgg
     2221 tcgcctgcgg gcgcccgtcg cgcaggatgt tcctgcggcg gaactcgacc ggatccagtt
     2281 tcagctcgcg ggccaccatg tcggtgtggc tctcgtacgc ccacaccagc tgcgggatgc
     2341 cgaagccgcg cagggcgccg gcggcgggaa ggttggtgta gagggcgtag gagtcgatga
     2401 gcacgttctc gatgtcgtag gggcccggcg cggtgaagcc ggacttctgc gtgatgcgcg
     2461 gcccgatgtc ggcgtaggca cccccgttcc accatacttc gcacagccgg ccggtgatgc
     2521 gcccgttctt cgtcacgccg ctcttgattc tcagcgttgc cgcgtgcttg gtgagcgtga
     2581 agaactgctc ctccatcgtg agcgaaacct tgaccgggcg gccggtcagc agtgcggcgg
     2641 cggcgaccag ggcctcgagc ttgatgtaca gcttcgcgcc gaaaccaccg cccaggtacg
     2701 gcaccttcac gcgcaccttg ttctcgggcc agccgagcag gcgcgcaatt tcgatgcgca
     2761 cgaacgaggg actttgcgaa gcagtatgga tcgtgatcgt gccgtcgccc ggcacggcgg
     2821 ccgagacgaa cggctccagc ggcacgtgca gcacctgctg cgtgcggaag gtatgctcga
     2881 acacgcggtc cgctttcctg aacgccgctt ccgcgtctcc gcgccgcagg tgatagtcga
     2941 gcgcgatgtt ggtgttcttc ctgccctgca ggtgtttgag atcggggaac gtcccggccg
     3001 gcttcagaac ctcgtgcacg atcgccttcg aggtcatcgc ctcgacctcg tcgtataccg
     3061 ccggcagttc ctcgtattcg gcggcgatcc tgcccgcagc ctcctccgcg acgtgcggat
     3121 cgtccgcaag caccagcgcg accggctcgc ccgcgtaacg caccttgtcg agcgcgagaa
     3181 tgggctgatc gtggaatgca ggaccgtaat gcgggtccgg gatgatcgcg cgaatgtcgg
     3241 caccggtgat gacgcggtgc acgccctcga cggctcgcgc ggggccggtg tcgatgcgct
     3301 tgatgcgccc gtgcgcgacc gtgctgcgga agatcttgcc gtgcagcatc cccggcagcc
     3361 gcaggttatg cacgtactcc gcgcgcccag tcaccttcgc ccggccctcg agccgtggga
     3421 tggaacgccc taccggtccg ctttgtgctg ttttcgccat ctatccgtga atcgtcaatc
     3481 gtaaaacgtg aatcgaaaac ccgtcactcg tcaatcgtca ctggcctgcg agcgcggcgc
     3541 gaactgcccg cgcgaggtaa acgcgaacga gttcgcgctt ataggccgcg gaaccgtgcg
     3601 cgtcgccgat cacgccggat tcggcagcag ctgcctcccc ggcgcgtttc aacaatgctt
     3661 catcggctcc ggcgccgcgc aaaacagatt ccgccttgat caggcgcgtg ggcgtgtcgg
     3721 tcgccgcgct gatgacgata tgagcatcgc ggattgcagc tccatccatg tcaagcacca
     3781 ccgccacgcc cagcgccggc cagtcgtcgg cggatcgcgt ggtgcatttc aggtacgcgg
     3841 cgcggcgcgc gccctgccgc gggacgatta cttcggcgat gagctctccg cgcccgagcg
     3901 tcgtttcgag gtagccggca tgcagcttct ccagcggcat cgtgcgctcg ccctgggcgc
     3961 ccacgatgac cgcgcgcgcg ccgagcgcga tcagcaccgg cggcaaatcc atgtgcggat
     4021 cgccgtgcgc aagatgcccg cccacggtcg cgacgttgcg cacgcgcacg ttggccaggg
     4081 tattgagcgt gcgggcgatg accggagctt ccttgtggat gaccggcgag cgctcgagcg
     4141 cggccagcgt aaccagtgcg ccgatgcgca gttccccatc gggaccgatc gcgatatcgg
     4201 catagcgtgg ctcgaccgcg cgcaggctga cgagccgggc cggccgcagc acccccagtt
     4261 tcatcatcag catgacggca gtgccgcccg cgaccgggcg ggccgagggc tcgcccgagc
     4321 ccagcagcgc cgccgcctcg cgcagtgacc gcggctcgag caattcaaac ggcgtcatag
     4381 gaggggcact ttactgataa cacccagaaa attcaaggaa ataacggctc atgtgtggta
     4441 gaatcctgcc cttttgcatc gcaccaatta ccgatacctc tttatgcgcg ccatccgcaa
     4501 catcgcgatt atcgcccacg tcgaccacgg caagaccacg ctggtcgaca agctgctgcg
     4561 ccagtccggc acgttcgccg cgcaccagca cctggtcgag cgcgtgatgg actcgaacga
     4621 cctcgagcgc gagcgcggca tcaccatcct cgccaagaat tgcgccgtca cctacgccgg
     4681 cacccacatc aacatcgtcg acaccccggg ccacgccgac ttcggcggcg aggtcgagcg
     4741 cgtgctgacc atggtggacg gcgtgctgct gctggtggac gcggtcgacg gcccgatgcc
     4801 gcagacgcgc tttgttaccc gcaaggcgct cgcccagggt ctcaagcccg tggtggtgat
     4861 caacaagatc gaccgtcccg gtgcccgccc ggactgggtc gtcaatcaca cgttcgacct
     4921 gttcgacaag ctcggcgcca ccgaggcgca actcgacttc cccatcgtgt acacctccgc
     4981 cctgaacggc acggcaaccc gggacctggc aaagcccggc accgacatgc gcccgctgtt
     5041 cgaggcgatc ctcgagcagg tgccggtgcg cgccgacgat cccgacggcc cgctgcaact
     5101 gcagatctgc tcgctcgact attcaagcta cgtcggccgc atcggcattg ggcgcgtcaa
     5161 ccgcggccgg atccgttccg ggcagcaggt ggcggtgctg cacggcccgg acagcacgcc
     5221 ggtcatcgcc aaggtcaacc aggtgctggt attcaagggg ctcgagcgcg tgctcgccga
     5281 agaagcgcag gcgggcgaca tcgtgctggt caacggcatc gaggaagtcg gcgtcggcgt
     5341 cacgctcgcc gaccccgcgc gcccggaagc gctgccgctg ttgaaggtgg atgagcccac
     5401 gctcgtcatg aatttccagg tcaatacctc gcccttcgcg gggcgcgaag gcaagttcgt
     5461 caccagccgc cagttgcgcg agcggctcgc gcgtgaagtc atgagcaacg tcgcgctgcg
     5521 cgtcgaggac accgaggatc ccgatgtgtt caaggtatcg gggcgcgggg agctgcacct
     5581 caccatcctg ctggagaaca tgcgccgcga aggttacgaa ctcgcggtgt cacgcccgcg
     5641 cgttctgttg aaggaaatc
//
LOCUS       DBNGSG010000366        14189 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1306739, whole genome shotgun sequence.
ACCESSION   DBNGSG010000366 DBNGSG010000000
VERSION     DBNGSG010000366.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 14189)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14189)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14189
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1306739"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(379..1671)
                     /locus_tag="AC158Q_09505"
     CDS             complement(379..1671)
                     /locus_tag="AC158Q_09505"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR02086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HIR3340052.1"
                     /translation="MGSTIAEKILGSHAGSGPAKAGDVVVASVDFAMLHDARASNALK
                     RIADFGTGKLPFAARTAFVLDHYSPPPNQEAANIHREMRAFADRHGVVFYDVGDGICH
                     QVLPEGGHFTCGDLVTGTDTHSTTYGAFNAFGTGVEGTDLSAVLMTGKLWFRVPETIR
                     VDLKGRLQPGVWAKDLTLHMLGRLGAEGANYRAIEYAGPAVAAMEIDDRMTLSNHAAE
                     LGAKAALLEADAKTIEWLRARGAREPKPVKADADARYCERFEIDASRLPPQVARKHRV
                     DDVVGVPEVKGQRIHYALIGTCTNGRLDDMRQAAAILKGRRIAKGVRMIITPASRNVY
                     LQAAHEGLIEVLTQAGASVEAAGCGTCVNITGHLVTGDGDVVITSANRNFKGRLGNAN
                     SEIFIGSSATVAASALTGTITDPREVEGGQWRPVPKAA"
     gene            1810..3231
                     /locus_tag="AC158Q_09510"
     CDS             1810..3231
                     /locus_tag="AC158Q_09510"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3340053.1"
                     /translation="MTEPASLTAYVAQFVLDTRASRIPADVMHLGKRSILDGIGLALA
                     GNAAESGHIVRTYLKSLGIATGKGATVIGTALKVPARFAAFANGIAIHADDYDDTQLA
                     VAKDRVYGLLTHPTAPALPPVLALGEIGNRSGLDVLTAYQVAVEAETKISEAINPRHY
                     DHGFHSTATIGAIAAAAGAARMMKLNLEQTRRALGLGASQSAGLRENFGTMTKPFHPG
                     RSAESGVVAAEFAKMGWTATPIVLEAGRGFFKAAGGGYDPAAIEGKLGNPWTFAFPGV
                     SIKPHPSGSLTHPGMGLMLDLIRRHDIRPEQVVKVKVGTNRHMPNALIHHRPTNELQA
                     KFSMEFCMAILLLERRAGLAEFTDKVVNSLKAKKMIERIEFGVHPEAEAAGYEKMTTI
                     IDIELAGGRRLGGRADFGKGSPSNPMTDDELAAKFRECAAWGKLPKASADKVVDKVFN
                     LEKLKSIRDLTRLLALGGRNSKK"
     gene            3346..4356
                     /locus_tag="AC158Q_09515"
     CDS             3346..4356
                     /locus_tag="AC158Q_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PdxA family dehydrogenase"
                     /protein_id="HIR3340054.1"
                     /translation="MKKPVIGLMLGDATGIGPEISAKLLASGVTAGLANVVVVGDKRV
                     LDLGIRNAGVALAYRVVPSIDAIRWPSETLPVIDLGNIDPARFPQGVSSAESGKLTGD
                     TLKFMIDLAMAGQLDGICFAPLNKGAMHQGGWKFNDEHQMFAKLTGHSGFFGEMNVIR
                     EFSTFRVTSHVALREAVNMISAERIESAIRLADDTLRSAGHANPRLGIAALNPHNGEN
                     GLFGDEEIRIIRPTVERLRAAGVNCTGPISSDAIFIKARNGEFDGVVMMYHDQGQIAT
                     KLLGFNKGVTVTAGLQTVFTTPAHGTAYDIVGKGKAERGAMEHAFTLAAKMAASRLAA
                     RH"
     gene            4399..5355
                     /locus_tag="AC158Q_09520"
     CDS             4399..5355
                     /locus_tag="AC158Q_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3340055.1"
                     /translation="MNIAKTLLVSAALAAATTAGAADFPSKSIRLVIGFAPGGGTDTT
                     SRALSTKLTAALGQQVIVDNRPGHSGTIAADILSKSTPDGHTLLLGTIALVINPILTK
                     TMPFDTFKDLLPVTQAVDSTNILIVHPSVAAKSVKDLIALAKTKPLNGGSSGIGGTGH
                     LAVELFNLQAGTKITHVPYKGGGPAMVDLLGGNIQLIFATAASSISHIKAGKARALAV
                     TTAKRSALVPDLPTVAEAGLPGYEANNWNGFFVPAKTPRPVVNRLNKEISTALLLPDI
                     KDFLFKQGLDAAPGTPEQFAKYMRSEYAKWTKVIRAAGLEAK"
     gene            5504..6430
                     /locus_tag="AC158Q_09525"
     CDS             5504..6430
                     /locus_tag="AC158Q_09525"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR3340056.1"
                     /translation="MFNPFQPLEKIKAEVYTSMPAKFRKKSRTAWSDPNRQGAEVECF
                     LEGPSFDRAGNLWIVDIPFGRIFRISPRQEWELVVQYDGWPNGLKFHKDGRAFITCYK
                     KGLLVLDPKTGKLETILETAFSESFKGLNDLHFASNGDLYFTDQGQTGIADPTGRVYR
                     LRANGALDRLVANVPSPNGITLSTTEKHCYVGVTRSNSVWRLPIMADGNVSKTGVAIQ
                     LSGGAAGPDGIEMDADNGLLVCHLGVGIWRFDANMLPTHLVYAEGHHHHHLDNLAFGG
                     PDSKTIYITEALSGDVLMAKMPVAGKKLYGLS"
     gene            complement(6499..7308)
                     /locus_tag="AC158Q_09530"
     CDS             complement(6499..7308)
                     /locus_tag="AC158Q_09530"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000098 - sulfur amino acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HIR3340057.1"
                     /translation="MDFLIEPGELLEQARQSDAVVVDTRKPAEYAKGHIPGAINFSTY
                     ELFALDTRAEGMTAFTRDVAARYAAAGVSSNRPVVLYEADTGMRAARDAWILQYLGHP
                     QVRLLHGGLAAWLAAGHGLSSEAADDWTVGMRVRERRELAIGFDEIARRMGRPGITLL
                     DVRDIDEHEGRDRTACCLRRGCIPGSIWIEWTQFLEAGRFKSPEAIRGLLHESGIDAE
                     SEIVPYCHRGARAANTCYALQHAGLPKVRNYIGSWHEWSSRSELPIEKTEG"
     gene            complement(7430..8308)
                     /locus_tag="AC158Q_09535"
     CDS             complement(7430..8308)
                     /locus_tag="AC158Q_09535"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3340058.1"
                     /translation="MFEGFKRFEIQTTDPQASIVGRVGGKGPPLLLLHGNPLTHVTWH
                     LVAPRLAKDFTVVCADLRGYGDSSKPRGLPDHTNYSFRRMGQDQMDVMEHLGFREFMI
                     AGHDRGARVAHRLALDYPERILKFASIEIIPTHWQLEKMKWTYAASGYHWFFFAQPYD
                     YPEQLLAGKEEYYIRWKFRKQGEGKFSPETMAEYVRCCTAEHIHGMAEDYRACLSVDY
                     PMDDADWKAGKRVACPLLPLWGEKTHTHKLFDINEAWVPYASNLLPAVKLPSAHYPME
                     EAPEETYNALSAFFNG"
     gene            complement(8320..9204)
                     /locus_tag="AC158Q_09540"
     CDS             complement(8320..9204)
                     /locus_tag="AC158Q_09540"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3340059.1"
                     /translation="MYEGFERFEIQTTDPEVKIVGRVGGKGPAVLLIHGNPLTHVHWH
                     LVAPRLAKDFTVVATDLRGYGDSGKPRGRPDHSNYTFRRMGQDQVDVMAHLGFGQFLV
                     AGHDRGARTAFRMALDHPDKVLKFASFDILPTHHMLTHATYQWALNSSHWFFMAQKHP
                     IPETLISGKEDFYIMKKLTDLGIGKGGFSRETLAEYARVCTPENIHSVCEDYRANVSL
                     DMAMDTVDFEAGRKIGCPVAVYWGELSHTEKYFDPRKAWPQYCSNIARIRPLPCGHYP
                     AEQVPDEVYKELREFFGN"
     gene            9387..10445
                     /locus_tag="AC158Q_09545"
     CDS             9387..10445
                     /locus_tag="AC158Q_09545"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HIR3340060.1"
                     /translation="MKILVLPGDGIGPEITDATLAVLDRANAKFKLGLEWQTMDIGLK
                     TLKAQGSTLPDSVMDAARKAPGIILGPVDHLQYPPREQGGINPSGELRVKLDLFANIR
                     PAKSRMGVGLTGKPVDLVIFRECTEGFYADRNMFSGRGEFMPTEDMALAVRKVTARQC
                     RRISEVAFAWARKRRKKVTAVHKANVLHLSDGLFLREVRKVAEQYPDVALDEKIVDAM
                     AALLVRNPTQFDCIVTGNMFGDILSDEASEISGSLGLAGSTMAGDDACCAQAQHGSAP
                     DIQGQDKANPTSLILSAAILFEWLAARHRRPEFEQAAKAIESAIDTSLKDAATRTADL
                     GGKLGTKAFAATVAARIG"
     gene            complement(10574..12055)
                     /gene="gabD"
                     /locus_tag="AC158Q_09550"
     CDS             complement(10574..12055)
                     /gene="gabD"
                     /locus_tag="AC158Q_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - GABA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HIR3340061.1"
                     /translation="MLQKVKPAVQLKDMSLFREQCYIDGEWVGADDKSTFAVYNPADG
                     QQIGTVPNLGAAETRRAIETANAAWPAWRAKSAKERAAILRKWYDLMMANQEDLAILM
                     TVEQGKPLTESRGEVAYGASFIEWFAEEGKRVYGDTIPAQSPDKRIVVIKQPIGVCAA
                     VTPWNFPNAMITRKAGPALAAGCTMVIKPASMTPYSALALCELAGRAGIPKGVISVVT
                     GSAGVIGKELTTNPLVRKFTFTGSTEVGKKLMEQCASTVKKVSLELGGNAPFIVFDDA
                     DLDAAVEGAMASKFRNTGQTCVCANRIYVQDGVYEKFAAKLGEKVGAMKVGNGLEAGV
                     AQGPLIEKKAVEKVEEHIADALAKGAKVLTGGKRHEKGGQFFQPTVLTGVTTSMKITR
                     EETFGPVAPLYRFKGETELIGLANDTEYGLAAYFYSRDIGRVWRVAEAIEAGIIGINV
                     GIISTEVAPFGGMKESGIGREGSKYGMEEFLEVKYLCMGGIDK"
     gene            12223..13203
                     /locus_tag="AC158Q_09555"
     CDS             12223..13203
                     /locus_tag="AC158Q_09555"
                     /inference="COORDINATES: protein motif:HMM:NF016236.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0120010 - intermembrane phospholipid
                     transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaA family lipoprotein"
                     /protein_id="HIR3340062.1"
                     /translation="MTFPHRSLPPRLRTLFLCLTLCIFVLGGCASTGNPRDPFEPINR
                     GIYKFNDTVDGLLIKPAATLYRDLLPQIVRTGISNFFSNLNDVIVALNNLMQGKVETA
                     INDMGRVLVNTTLGVLGVMDPATKLGVEKNNEDFGQTLGFWGLGDGPYIVLPFLGPSS
                     LRDTVGWVGDVYTWPVTYLEPDSHRNAVIGLRFVGARADLLEASKIRETAALDPYEFV
                     RDAYLQRRRNLVYDGNPPDDSGDDSREKPASQKPSSRGPASPPVPVAAAAGQEVALTA
                     ESVPLSTGDAARESEPAASPETAPQPQGADSEPATPQVSSDVRIWLPDRN"
     gene            complement(13352..>14189)
                     /locus_tag="AC158Q_09560"
     CDS             complement(13352..>14189)
                     /locus_tag="AC158Q_09560"
                     /inference="COORDINATES: protein motif:HMM:NF024762.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SirB1 family protein"
                     /protein_id="HIR3340063.1"
                     /translation="MSHAERWKEIVAGPEEDIDLAEGALLIAAPEYPGLDIPDCLARL
                     AELAAALKRRLRGDIGPTDTLIALNHYLFDELGFRGNAEDYYDPRNSFLNEVLERRVG
                     IPITLSLVHVEIGRRIGLAMHGVAFPGHFLVKCVLRDGAVVLDSYARGASLGLEELQQ
                     RLKVMRGGAMPSPDMVRHMLAAAGKKSMLARMLRNLKGIYRERGDLDRALAAAGRIIE
                     LEPGAAEEYRDRAGLYQELECYRAALSDFRNYLMLNPRAEDAAVVQRRVVELQQIASR
                     LN"
BASE COUNT         2493 a         4817 c         4368 g         2511 t
ORIGIN      
        1 cagcgaaacc gggcgcaagc tgctcgaagg catgctgcgc cacgtaggcg ttgtcccggc
       61 ccgggtggta cttgctcgag cagtggagat cggtgttgat ctcgtcgccg agcaggtagg
      121 ccttgccgtg tatcagtgtc gtcatgtgca aaccagaatc aagaaatgga caggattaac
      181 aatatgtttt caaatcgaaa acccgtcaac ttctccaata gacaggatta acaagattta
      241 caggattaaa tccgaatcaa gaaccaaaac gtggtttctg tttgaagttt gaatcttgtt
      301 aatcgtgtta atcctgtcta ttacgggctt ttatgcgggt tttgagcttc gtaatttgag
      361 ttctgtaaat cctgtccatt aagctgcctt gggcaccggg cgccactgcc cgccctcgac
      421 ttcgcgcgga tcggtgatcg ttcccgtcag cgccgaggcg gccaccgtgg cggacgagcc
      481 gatgaagatc tcggagttcg cgttgcccag ccgtcccttg aagttgcggt tcgcgctggt
      541 gatcaccacg tcgccgtcgc ccgtgaccag gtgcccggta atgttgacgc aggtgccgca
      601 gcccgcggcc tcgaccgagg cgcccgcctg cgtgaggacc tcgatcagcc cctcgtgcgc
      661 cgcctgcagg tacacgttgc gcgaagccgg cgtgatgatc atgcgcacgc ccttcgcgat
      721 gcgccggcct ttcagtatcg cggccgcctg ccgcatgtcg tccagccgcc cattggtgca
      781 ggtgccgatc agcgcgtagt ggatgcgctg gcccttgacc tcgggcacgc cgaccacgtc
      841 gtccacgcga tgcttgcgcg ccacctgcgg cggcagcctg gaggcgtcga tctcgaagcg
      901 ctcgcagtag cgcgcgtcgg catcggcctt gaccggcttc ggctcgcgcg cgccgcgcgc
      961 ccggagccac tcaattgtct tggcatccgc ttccagcaac gccgccttcg cgcccagttc
     1021 cgccgcgtgg ttggaaagcg tcatgcggtc gtcgatctcc atcgcggcga ccgccggacc
     1081 cgcgtactcg atcgcccgat agttcgcccc ttccgctccc agccgcccga gcatgtgcag
     1141 ggtcaggtcc ttggcccaca cccccggctg cagccgcccc ttgagatcga cgcggatcgt
     1201 ctcgggaacg cggaaccaga gcttgccggt catcagcacc gccgagagat cggtgccttc
     1261 gacgccggtg ccgaaggcgt tgaacgcgcc gtacgtggtc gaatgcgtgt cggtgcccgt
     1321 caccaggtcg ccgcaggtga aatgcccgcc ttcgggcagc acctggtggc agatgccgtc
     1381 gccgacgtcg tagaacacga cgccgtgcct gtccgcgaac gcccgcatct cgcggtgtat
     1441 gttggccgct tcctggttgg gcggcgggga atagtggtcg agcacgaacg cggtgcgcgc
     1501 ggcgaacggg agcttgccgg tgccgaagtc cgcgatgcgc ttcaacgcgt tgctcgcgcg
     1561 cgcatcgtgc agcatggcga aatccacgct ggcgacgacc acgtcgcccg ccttcgccgg
     1621 tccgcttcca gcatgcgaac cgaggatttt ttcggcaatc gtggagccca tagtgctcgt
     1681 ctctgaggga gggttgttat tttacaggct tactccggcc gcggaaccaa agttcactat
     1741 ctggcacgca gccgccagat gtggtattta gagcctcttc tcagcaggct cttctaccga
     1801 aaaacagtca tgaccgaacc cgcttccctc accgcttacg tcgcgcaatt cgtcctcgac
     1861 accagagctt cccgcattcc cgccgatgtc atgcacctcg gcaagcgttc gatcctcgac
     1921 ggcatcgggc tcgcgctcgc cggcaacgcg gccgagtccg gccacatcgt ccgcacctat
     1981 ctgaaaagtc tcggcatcgc gaccggcaag ggcgcgaccg tcatcggcac ggccctgaaa
     2041 gtgccggcgc gctttgccgc cttcgccaac ggtatcgcga tccatgccga cgactacgac
     2101 gacacccagc tcgccgtcgc caaggaccgc gtctacggcc tgctcaccca tcccaccgcg
     2161 cccgccctgc cgccggtgct ggcgctgggc gagatcggca accgctcggg actcgacgtg
     2221 ctgaccgcct accaggtcgc ggtcgaagcc gagaccaaga tctccgaggc gatcaacccg
     2281 cgccattacg accacggttt ccactccacc gccaccattg gcgcgatcgc cgccgccgcc
     2341 ggcgccgccc gcatgatgaa gctcaacctc gagcagaccc gccgcgcgct tgggctaggc
     2401 gcgagccagt cagcagggct gcgcgagaat ttcggcacca tgaccaagcc gttccatccc
     2461 gggcgctcgg ccgagagcgg cgtcgtcgcc gcggagttcg cgaaaatggg ctggaccgcg
     2521 acccccatcg tgctggaagc cggccgcggc ttcttcaagg cggcaggcgg cggttacgat
     2581 cccgccgcga tcgagggcaa gctcggcaac ccgtggacct tcgcctttcc cggcgtctcg
     2641 atcaagccgc atccgtcggg ctcgctcacc catccgggca tgggcctcat gctcgacctc
     2701 attcgcaggc acgacatccg gccggaacag gtcgtgaagg tcaaggtcgg caccaaccgc
     2761 cacatgccga acgcgctgat ccaccaccgc ccgacgaacg agctgcaggc gaagttcagc
     2821 atggaattct gcatggcgat cctgctgctc gagcgaaggg cgggactcgc cgagttcacc
     2881 gacaaggtgg tgaacagcct gaaggcgaag aagatgatcg agagaatcga gttcggcgtc
     2941 caccccgagg ccgaggcggc gggttatgag aaaatgacca cgatcatcga catcgagctg
     3001 gcgggcggcc gcagactcgg cggccgcgcg gacttcggca agggcagccc ttccaacccc
     3061 atgaccgatg acgagctcgc cgccaagttc cgcgagtgcg cggcctgggg caagctgccg
     3121 aaggcgagcg cggacaaggt ggtggacaaa gtcttcaacc tggagaagct gaagagcatc
     3181 cgcgacctga cccgcctcct cgccttaggc ggcaggaaca gcaagaaata gacaggatta
     3241 acaagattaa acaggattta catgattcca gaattgaatt ccgtcctgtt aatcttgtga
     3301 aatcctgtaa atcctgtcca tttttttgat ttttctcgcg cgcttatgaa gaaacccgta
     3361 atcggcctga tgctcggcga cgccaccggc atcggccccg aaatcagcgc caagctgctc
     3421 gcgtcgggcg tgacggccgg gctggcgaac gtcgtcgtgg tcggcgacaa gcgcgtgctc
     3481 gatctcggca ttcgcaacgc gggggttgcc ctcgcatatc gtgtcgttcc ctcgatcgac
     3541 gccatccgct ggccgtccga aacgctcccg gtcatcgacc tgggcaatat cgatccggca
     3601 aggtttccgc agggcgtctc gtccgccgag tccggaaagc tgaccggcga caccctgaag
     3661 ttcatgatcg atctcgcgat ggcgggccag ctcgacggca tctgcttcgc gccgctcaac
     3721 aagggcgcca tgcaccaggg cggctggaaa ttcaacgacg agcaccagat gttcgcgaag
     3781 ctgaccggcc acagcggctt tttcggcgaa atgaacgtga tcagggaatt cagcaccttc
     3841 cgcgtaacct cccacgttgc gctgcgcgag gcggtcaaca tgatctcggc ggagcgcatc
     3901 gagagcgcga tccgcctcgc ggatgacacc ctgcgctcgg ccggccacgc caatccgcgc
     3961 ctgggcatcg ccgcgctcaa tccgcacaac ggcgagaacg ggctgttcgg cgacgaggag
     4021 atccgcatca tccgcccgac ggtggagcgg ttgcgcgcgg cgggcgtgaa ctgcaccggc
     4081 ccgatctcgt ccgacgcgat cttcatcaag gcccgcaacg gcgagttcga cggcgtggtg
     4141 atgatgtacc acgaccaggg ccagatcgcg accaagctgc tgggcttcaa caagggcgtc
     4201 accgtcaccg ccgggctcca gaccgtcttc accacccccg cacacggcac cgcctacgac
     4261 atcgtcggca agggcaaggc cgaacgcggg gcgatggagc atgctttcac gctcgcggcg
     4321 aaaatggcgg catcccgcct cgccgcgcgc cattgaaccg acctataatc gaccgctgaa
     4381 tcacacgagg agaacgacat gaacatcgcc aagacattgc tggtttcggc ggccctggcc
     4441 gccgcaacga ccgcgggtgc cgcggacttt ccgagcaagt cgataagact cgtcatcggg
     4501 ttcgcgccgg gcggcggcac cgacaccacc tcgcgcgccc tcagcaccaa gctgacggct
     4561 gccctcggcc agcaggtcat cgtcgacaac cgcccggggc attcgggcac cattgccgcg
     4621 gacatcctgt cgaaatcgac gccggacggg cacaccctgc tgctcgggac gatcgcgctc
     4681 gtgatcaatc ccatcctgac gaaaacgatg ccgttcgata ccttcaagga cctgctgccg
     4741 gtgacgcaag cggtggactc gaccaacatt ctcatcgttc acccctcggt ggccgccaag
     4801 tccgtgaaag acctgatcgc gctcgccaag acgaaaccgc tgaacggcgg ctcctccggc
     4861 atcggcggca cggggcacct ggcggtggag ctgttcaatc tccaggccgg caccaagatc
     4921 acccacgtcc cgtacaaggg cggcggcccc gcgatggtgg acctgctggg cggcaacatc
     4981 cagctgattt tcgccaccgc ggctagctcc atctcgcata tcaaggcggg caaggcgcgg
     5041 gcgctggccg tgaccacggc gaagcgctcg gccctggtgc cggatctgcc gacggtcgcg
     5101 gaagcaggac tgcccggcta cgaggcgaac aactggaacg gctttttcgt cccggcgaaa
     5161 acgccgcgcc cggtcgtcaa ccggctcaac aaggagatct cgaccgcgct cctgctgccc
     5221 gacatcaagg acttcctgtt caagcagggc ctcgacgccg cgccgggcac gccggagcaa
     5281 ttcgccaagt acatgagatc cgagtacgcc aagtggacga aagtgatccg ggcggcgggt
     5341 ctcgaagcca aatagcgtta aaccggacgt ggagcgccgg tttaactccg cgcgatcgag
     5401 cggacggcca atccgccgct catcgcgcgg accgttcgtc actcatcact catcaatcat
     5461 cactcgaccc tcgaccctcg acactcgacc cccaacgtta agcatgttca accccttcca
     5521 gcccctcgag aaaatcaagg ccgaggtcta tacctcgatg cccgcgaagt tccgcaagaa
     5581 gagccgcacc gcctggtccg atcccaatcg ccagggcgcg gaagtcgaat gtttcctgga
     5641 aggcccctcc ttcgaccgtg ccggcaacct ctggatcgtg gacattccct tcggccgcat
     5701 cttccgcatc agccccaggc aggaatggga actcgtcgtg cagtacgacg gctggcccaa
     5761 cggcctcaag ttccacaagg acgggcgcgc cttcatcacc tgctacaaga aggggctgct
     5821 cgtgctcgac ccgaagacgg gcaagctcga gacgatcctg gaaaccgcct tcagcgagag
     5881 cttcaagggc ctgaacgacc tccacttcgc ctcgaacggc gacctctact tcaccgacca
     5941 ggggcagacc ggcatcgccg acccgaccgg gcgcgtctac cggctgcgcg cgaacggcgc
     6001 gctcgaccgg ctcgtcgcca acgtgccgag ccccaacggc atcacgctct ccaccaccga
     6061 gaagcactgc tacgtcggcg tcacgcgctc gaactcggtc tggcgcctgc cgatcatggc
     6121 cgacggcaat gtgtcgaaga ccggtgtcgc gatacagctc tccggtggcg ccgccggtcc
     6181 cgacggcatc gagatggacg cggacaacgg cctgctggtg tgccacctgg gcgtcggcat
     6241 ctggcgcttc gacgccaata tgctgccgac ccacctcgtc tacgccgagg gacatcacca
     6301 ccatcacctg gacaaccttg ccttcggtgg cccggattcg aagacgatct acatcaccga
     6361 ggcgctctcc ggcgacgtgc tgatggccaa aatgccggtg gcggggaaga agctttacgg
     6421 cctgagctga agtgtgacaa gtgacgagtg acggttcccg cgcgaagcgc ggcttcctca
     6481 tccctcatcc ctccgccctc atccctctgt cttctcgatc ggaagctccg atcgagaaga
     6541 ccactcgtgc caggagccga tgtagttgcg cactttcggc aatccggcgt gctgcagcgc
     6601 atagcaggtg ttggcggcgc gcgcgccgcg gtggcagtag ggcacgattt ccgattccgc
     6661 atcgatgccg ctctcgtgca gcaggccgcg tatcgcctcc ggtgacttga agcgaccggc
     6721 ttcgagaaac tgcgtccatt cgatccagat cgacccggga atgcaaccgc ggcgcaggca
     6781 gcacgcggtg cggtcgcggc cctcgtgctc gtcgatgtcg cgcacgtcga gcagcgtgat
     6841 gccgggtcgc cccatgcgcc gcgcgatctc gtcgaagccg atggccaatt cccggcgctc
     6901 acgcacgcgc atgcccacgg tccagtcgtc cgcggcctca gagctcaggc catggcccgc
     6961 cgcgagccac gccgccagtc cgccgtgcag cagccggacc tgcgggtggc cgaggtactg
     7021 caggatccag gcgtcgcgcg cggcccgcat cccggtatcc gcttcataga gcacgaccgg
     7081 ccggttgctg gaaacaccgg cggcggcata gcgtgccgcc acgtcgcgcg tgaacgccgt
     7141 catcccctcc gcgcgcgtat cgagcgcgaa gagctcgtaa gtgctgaagt tgatcgcgcc
     7201 cggaatatgg cctttcgcgt actccgccgg cttgcgcgtg tcgacgacca cggcatccga
     7261 ttgccgcgcc tgctccagca gttccccggg ctcgatcagg aagtccatgg cttcaaatgc
     7321 gtctcagata cggcgtcgca ggtacggcga gtttggctgc gcctcatttg agacgccttt
     7381 cctgagacgc ttcacctgag acgccttatc tgcgacgctc ttcaggctgt cagccattga
     7441 agaaagctga cagcgcgttg taggtctcct ccggcgcctc ctccatcggg tagtgcgccg
     7501 atggcaattt tacggccggg agcaggttcg aggcataagg gacccacgct tcgttgatgt
     7561 cgaagagttt gtgcgtgtgg gtcttctcac cccacagcgg cagcagcggg caggccacgc
     7621 gcttgccggc cttccagtcc gcgtcgtcca tcgggtaatc cacggagagg caggcgcgat
     7681 agtcttccgc catgccgtgg atatgctcgg ccgtgcagca gcgcacgtat tccgccatcg
     7741 tctcggggct gaacttgccc tcgccctgct tcctgaattt ccagcggata tagtactcct
     7801 ccttgccggc cagcagctgt tccgggtaat cgtacggctg cgcgaagaag aaccagtggt
     7861 agccgctcgc cgcgtaggtc catttcatct tctcgagctg ccagtgcgtc gggatgatct
     7921 cgatgctcgc gaatttgagg atacgctccg ggtaatccag cgccaggcgg tgcgcgacgc
     7981 gcgcgccgcg gtcgtggccg gcgatcatga attcccggaa tcccaggtgc tccatgacgt
     8041 ccatctggtc ctggcccatg cgccggaagc tgtagttcgt gtggtcgggc aggcccctgg
     8101 gcttggagct gtctccatag ccgcgcaggt cggcgcaaac aacggtgaaa tccttcgcga
     8161 gccgcggcgc gacaagatgc caggtcacgt gcgtcagcgg attgccatgg agcagcagca
     8221 gcggcggccc cttgcccccg acgcggccga cgatgctcgc ctgaggatcg gtggtctgta
     8281 tttcgaaccg cttgaatccc tcgaacatgc cggtctcctt caattgccga agaactcgcg
     8341 gagttccttg tagacctcgt ccggcacctg ttccgccggg tagtggccgc agggcagcgg
     8401 gcggatgcgc gcgatgttcg agcagtactg cggccacgcc ttgcgcggat cgaagtactt
     8461 ctcggtatgc gagagctcgc cccagtagac cgccaccggg cagccgatct tgcggcccgc
     8521 ctcgaaatcc acggtgtcca tcgccatgtc caggctgacg ttggcgcggt agtcctcgca
     8581 cacgctgtgg atgttctccg gcgtgcatac gcgggcgtac tccgcgagcg tttcccggga
     8641 gaagccgccc ttgccgatgc ccaggtcggt gagcttcttc atgatgtaga agtcctcctt
     8701 gcccgagatc agcgtctccg gaatcgggtg cttctgggcc atgaagaacc agtgcgagga
     8761 gttgagcgcc cactggtagg tcgcgtgggt cagcatgtgg tgcgtgggca ggatgtcgaa
     8821 gctcgcgaac ttcaatacct tgtccgggtg atcgagcgcc atgcggaacg ccgtgcgcgc
     8881 gccgcggtcg tgccccgcga ccaggaactg cccgaagccg agatgcgcca tcacatccac
     8941 ctggtcctgc cccatgcgcc ggaacgtgta gttgctgtga tccggtcttc cgcgcggctt
     9001 gccgctgtcg ccgtagccgc gcagatcggt cgccaccacc gtaaaatctt tcgccagccg
     9061 cggcgcgacg aggtgccagt gcacgtgcgt cagcggattc ccgtggatca gcagcacagc
     9121 cggccccttg ccgccgacac gcccgacgat tttcacttcg gggtcggtgg tctggatttc
     9181 gaaccgctcg aagccttcgt acatgaatcc gcctttgcag tagataggga acgcacgatg
     9241 ttacaatcgc gccctccgca taacaatgcc tgcggcacga tcaccggcga tttcatcaat
     9301 tacattcctt tcatgcatat cgcaaagacc atttctgcat ttcctggcgt ccctggcgcc
     9361 ttggcggcta ttttccggag tttgccgtga agattctcgt gctgcccggc gacggcatcg
     9421 gccccgagat cacggacgcg acgctcgcgg tgctcgaccg cgccaacgcg aaattcaagc
     9481 tcgggctcga atggcagacg atggacatcg gcctgaagac gctgaaggcg cagggctcga
     9541 cgcttccaga ttcggtcatg gacgccgccc gcaaggcgcc cggcatcatc ctcgggccgg
     9601 tggaccatct gcagtatccg ccgcgcgagc aaggcggcat caacccttcc ggcgagctgc
     9661 gcgtgaagct cgatctcttc gccaacatcc gccccgccaa gtcgcgcatg ggcgtgggcc
     9721 tcaccggcaa acccgtggat ctcgtgatct tccgcgagtg caccgagggc ttttacgccg
     9781 accgcaacat gttcagcggg cgcggcgagt tcatgccgac cgaggacatg gcgctcgcgg
     9841 tgcgcaaggt gaccgcgcgc cagtgccgcc gcatctcgga agtggcgttc gcgtgggcgc
     9901 gcaagcgccg caagaaggtc accgcggtgc acaaggccaa tgtgctgcac ctctccgacg
     9961 gcctgttcct gcgcgaagtg cgcaaggtcg cggagcaata tccggacgtg gcgctcgacg
    10021 agaagatcgt ggacgcgatg gcggcgctgc tggtgcgcaa cccgacgcag ttcgactgca
    10081 tcgtcaccgg caacatgttc ggggacatcc tctcggacga ggcctccgag atctccggca
    10141 gcctcgggct cgcgggctcc accatggcgg gcgatgacgc ctgctgcgcg caggcccagc
    10201 atggctccgc gcccgacatc cagggccagg acaaggccaa ccctacttcc ctcatcctgt
    10261 cggcggcgat tctcttcgag tggctcgccg cgcggcaccg gcgtcccgag ttcgagcagg
    10321 ccgcgaaagc gatcgaatcg gccatcgaca cttcgctcaa ggacgccgcc acccgcaccg
    10381 ccgacctcgg cggcaagctc ggcacaaaag ccttcgccgc gacggtcgcg gcgaggattg
    10441 ggtgatccgc gatgagtgac gagtgacgaa aaccggatcg aatcacgact cacgaatcac
    10501 gactcacgcc gttcccgttc cgcaataaaa acggcacctc tcgaggtgcc gtttttttgc
    10561 tggcggagcc gggttacttg tcgatgccgc ccatgcaaag atacttcacc tcgaggaact
    10621 cctccatgcc gtacttggag ccctcgcggc cgatgccgga ctccttcatg ccgccgaacg
    10681 gcgcgacctc ggtcgagatg atgccgacgt tgatgccgat gatgccggcc tcgatcgcct
    10741 ccgccacgcg ccagacgcgg ccgatgtcgc ggctgtagaa atacgccgcg agcccgtact
    10801 cggtgtcgtt ggcgagcccg atcagctcgg tttcgccctt gaagcggtag agcggcgcca
    10861 ccggcccgaa ggtttcttcg cgcgtgatct tcatcgaggt cgtgacgccg gtcagcaccg
    10921 tgggctggaa gaactgcccg cccttctcgt gccgcttgcc gccggtcagc accttcgcgc
    10981 ctttcgccag cgcgtcggcg atgtgctcct cgaccttttc caccgccttc ttctcgatca
    11041 gcggcccctg cgcgacgccg gcttccaggc cgttgcccac cttcatcgcc ccgaccttct
    11101 cgccgagctt ggccgcgaat ttttcgtaga cgccgtcctg cacgtagatg cggttggcgc
    11161 agacgcaggt ctggccggta ttgcggaact tggaggccat cgcgccctcg accgcggcat
    11221 cgaggtcggc gtcgtcgaac acgatgaagg gcgcattgcc acccagctcg agcgacacct
    11281 tcttcacggt gctcgcgcat tgctccatca gcttcttgcc gacctcggtc gagccggtga
    11341 aggtgaactt cctcaccaac gggttggtcg tgagttcctt gccgatgacg ccggcgctgc
    11401 cggtgaccac cgagatcacg cccttgggaa tgccggcgcg cccggccagc tcgcacaacg
    11461 cgagcgccga gtacggcgtc atgctggcgg gcttgatcac catggtgcag ccggcggcga
    11521 gcgcggggcc cgccttgcgg gtgatcatgg cgttggggaa gttccacggc gtcaccgccg
    11581 cgcacacccc gatcggctgc ttgatcacca cgatgcgctt gtcgggcgac tgggccggga
    11641 tggtgtcgcc gtagacgcgc ttgccctcct cggcgaacca ctcgatgaag gaagcgccgt
    11701 aggcgacctc gccgcgcgac tcggtgaggg gcttgccctg ctccaccgtc atcaggatcg
    11761 ccaggtcttc ctgattcgcc atcatcaggt cgtaccactt gcgcaggatc gccgcgcgct
    11821 ccttggcgct cttcgcgcgc caggccggcc acgcggcgtt ggccgtctcg atcgcgcgcc
    11881 gcgtctcggc cgcgcccagg ttgggcacgg tgccgatctg ttgcccgtcg gcggggttgt
    11941 agaccgcgaa agtggacttg tcgtcggcgc ccacccactc gccgtcgata tagcactgct
    12001 cgcggaacag gctcatgtcc ttcaattgca ccgcgggttt caccttttga agcatggctg
    12061 gattcctcgt gtcggatcgg gagcgtgcgg ggcggaaccg ccgcgcacga tgcgatgcag
    12121 tatagcagag cgcccgcgcg caaccggcgc atccgaccgg cagaaccggc tggccaccga
    12181 gtcgcacaag atcaaaaaat gattgatata ataagctctt atatgacgtt cccgcatcgg
    12241 tctctgccgc cgcgcctgcg cactctgttc ttgtgcctga cgctgtgcat ttttgtcctg
    12301 ggcggctgcg ccagcaccgg caacccgcgc gatcccttcg agccgatcaa ccgcgggatc
    12361 tacaagttca atgacaccgt cgatggcctg ctcatcaagc cggcggccac gctgtaccgc
    12421 gacctgctgc cgcaaatcgt gcgcaccggc atatccaatt tcttctccaa cctcaacgat
    12481 gtcatcgtcg cgttgaacaa cctgatgcaa ggcaaggtcg agaccgccat caacgatatg
    12541 gggcgcgtcc tggtcaacac cacgctcggc gtgctgggcg tgatggatcc cgccacgaaa
    12601 ctcggggtgg aaaagaacaa cgaggacttc ggccagacgc tcggattctg gggcctgggc
    12661 gacgggccgt acatcgtgct gccgttcctc gggcccagca gcctgcgcga taccgtcggc
    12721 tgggtcggcg acgtctacac ctggccggtc acttacctgg agcccgacag tcaccgcaat
    12781 gccgtgatcg ggctgcgttt cgtgggcgcc cgcgccgacc tgctggaagc cagcaagata
    12841 cgcgagaccg ccgcgctcga cccgtacgag ttcgtgcgcg acgcctacct gcagcgccgc
    12901 cgcaacctgg tgtacgacgg caatccgccg gatgacagcg gcgacgacag cagggagaaa
    12961 cctgcatcac agaagccttc gtcacgcggc ccggcaagcc cgcctgtgcc ggttgcggcg
    13021 gcagccggcc aggaggtggc gttgacggcc gaaagcgttc cgttatcgac cggcgacgcg
    13081 gccagggaga gtgagcccgc ggcatccccg gaaacggcgc cccagcccca gggcgccgat
    13141 tcagagccgg caacaccgca agtatccagc gacgttcgta tctggctgcc cgaccgcaac
    13201 tgagcggcgc cgcgcgccgc tgcgttgctc gtttctcgtt actcgttact cgtttctcgt
    13261 ttctcgttct ccgtttttcc caagccgttc ccgcctgggt tacgcgcaac actttatcga
    13321 gtaacgagga acgagcaacg agtcactttt tctagttaag tcgagaagcg atctgctgca
    13381 gctccaccac cctgcgctgc accaccgcgg cgtcttccgc gcgcggattc agcatgaggt
    13441 aattgcggaa atccgacagc gcggcgcgat agcattcgag ttcctggtaa aggccggcgc
    13501 ggtcgcggta ctcctccgcc gcgccgggct cgagctcaat gatacggccg gccgccgcca
    13561 gcgcgcggtc cagatcgccg cgctcccggt agatgccttt caggttgcgc agcatgcgcg
    13621 cgagcatgct cttcttgccg gcggccgcca gcatgtgccg gaccatgtcc ggcgagggca
    13681 tcgcaccgcc gcgcatcacc ttcagccgct gctgcagctc ttccaggccc agcgaggccc
    13741 cgcgcgcgta cgaatcgagc acgactgccc cgtcccgcag gacgcacttc accaggaaat
    13801 gcccggggaa cgcgacgccg tgcatcgcga gcccgatgcg ccggccgatc tcgacatgga
    13861 ccagtgacag cgtgatcggg atgccgaccc ggcgttcgag cacctcgttc aaaaagctgt
    13921 tgcgcgggtc gtaatagtcc tccgcgttgc cgcggaaccc gagctcgtcg aagaggtagt
    13981 gattcagcgc gatcagggtg tccgtcgggc cgatgtcgcc gcgcagccgg cgcttcagcg
    14041 ccgcggcgag ctccgcgagg cgcgcgaggc agtcgggaat gtcgaggccg ggatattcgg
    14101 gcgcggcgat gagcagcgca ccttccgcga ggtcgatatc ttcctccggg ccggcgacga
    14161 tctccttcca gcgttcagcg tgtgacatg
//
LOCUS       DBNGSG010000367         6295 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_628949, whole genome shotgun sequence.
ACCESSION   DBNGSG010000367 DBNGSG010000000
VERSION     DBNGSG010000367.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6295)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6295)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6295
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_628949"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="AC158Q_09565"
     CDS             <1..1164
                     /locus_tag="AC158Q_09565"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3340064.1"
                     /translation="GLGFNLAWPFVPLMVRGLGVRENLETWVGYMLLAFYLVSTTASP
                     LWGGIADHYGRKLMVLRAMLGMGVAMMLVPLAPTPVWFAVALMLVAVFNGFTPAGVSL
                     LVANTPPTRIGSAVSLAQTGGQVGHAMGPVVGAALAALIDRQHALYWISGGFMLSGGL
                     LVALLVREVKQLAPGPWRPRLVGSLRDLLGVPRIAPLFLLAFLFSIMWHGSITNISVF
                     MLQLLEIHQPGADMATEASWVGAAATAMALSMMFSLPLWGRVIDRIGPGRVLVFAAAA
                     SVVTHLPLLVLQTPLQLVLSRVLFGLTAAGMQTAIFQLLRMHAPPGTDARALSYATAF
                     QFLAMGMAPFTAGLIGPAFGLRAYFAVTIALMLGGLVLWLRTASRKPEVDSRK"
     gene            1230..2105
                     /locus_tag="AC158Q_09570"
     CDS             1230..2105
                     /locus_tag="AC158Q_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340065.1"
                     /translation="MPRSRIATAGYRRTRARPPGIPLVRPLFRFGRRALGKVGAAPLP
                     VRLIAGAAVILALFFALNWLVQVIRKPAELFFPVSGTLHKAPSETWRQYGPLFRGHST
                     AVMTPELLAALAQVEGAGNPVARTYWRWRLTWNPLELYQPASSAVGMYQITNAAFQDA
                     RRYCIHDHAVAEDGPWNDWRSCWFNSLYTRVIPSHAIEMTSAFLDRSVARTLARQRIA
                     AATFPQRQDLAAVIHLCGSGVGAVYARRGFQLTAAARCGDHDVRAYLARVNAMKRYFA
                     WRAAAREGRGTRDEG"
     gene            complement(2170..3021)
                     /locus_tag="AC158Q_09575"
     CDS             complement(2170..3021)
                     /locus_tag="AC158Q_09575"
                     /inference="COORDINATES: protein motif:HMM:NF012417.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3340066.1"
                     /translation="MALKYPLVHVDDVRPENMKQGEGWAISEFRLPISGKHGSSTTLF
                     HSIFRPGSTHARHLHRNCDEIAVYLKGHGVVGQGTERAQVRPGHCRLMPRGLEHFFHN
                     ETKDEEGLVIGFYVGARSVADTGYEFCGHATRADLEMPRVKQFSTGIHVHIDDVKPSR
                     MNAKDGWSITDFRLPIGRHNGSSTTLFWAKFMPGAVHKKHRHDRCEELYYVVRGRGLA
                     GAGPDRAEVRAGHVHYIPRGVEHFMHNLSRAEPLEVIGVYVGAGSVEETGSVFTGEVG
                     EADLTIR"
     gene            3199..4302
                     /locus_tag="AC158Q_09580"
     CDS             3199..4302
                     /locus_tag="AC158Q_09580"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012958.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3340067.1"
                     /translation="MPLKRAKGSAGTVVVLEHVSKILEGNPLGDPHSRKLAVWLPPQY
                     DDSGGRRFPVLYDFVGFGGSGLSHTGWKGFGYNVPERAARLIREGRMGPAILVFPDCF
                     TALGGNQYVNSTATGNYADYLTREIIPFVDREFRTLAAREHRGFFGKSSGGYGSMIHG
                     MKYAKYWGAIANHSGDAYFDFVYWCDWPNTLNELAKHRVPKRRPGRYDARREAGRRGL
                     SGGRDDGRIRRFLERVWKKEKVSTAEGHCIMNLCMAATYDPDPKAPLGFRVPVNLESG
                     EPIAERWRQWRRHDPIHLVEKYRASLRTLRGIYIDCGWRDQYHIHYGSRILSRRLAAA
                     GIRHTYEEFDDNHSDIDYRMDVSLPFLYRALKP"
     gene            complement(4399..4524)
                     /locus_tag="AC158Q_09585"
     CDS             complement(4399..4524)
                     /locus_tag="AC158Q_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340068.1"
                     /translation="MSGRGQTGHNQMLKRRRNKNYARKQLAKAAKRAKKLRNKKN"
     gene            complement(4611..5126)
                     /locus_tag="AC158Q_09590"
     CDS             complement(4611..5126)
                     /locus_tag="AC158Q_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340069.1"
                     /translation="MNRAAAISIGVAPFVLAIGLYATGTLEWMDNAIERHRWGPKAPH
                     AGPTRPLENYAGWWQREGIPNYGTEWVSRIIVRTEGKRAWLRMWHYCETKYCEQGEFE
                     ADVYGKPPDDVYALEVVRKKGKDVFWTITLRPNGDNPKSLVILDDRRARDPGKNPMDN
                     QSSFTALRRVK"
     gene            complement(5165..6157)
                     /locus_tag="AC158Q_09595"
     CDS             complement(5165..6157)
                     /locus_tag="AC158Q_09595"
                     /inference="COORDINATES: protein motif:HMM:NF014899.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DODA-type extradiol aromatic ring-opening family
                     dioxygenase"
                     /protein_id="HIR3340070.1"
                     /translation="MAQIVGVFQTSHSPFCYRRPEAWNDARKGRPIRPDVPMDDLETN
                     RKKYARIQDSFATLKRKVAQARPDVIVIFGDDQMECFDFSNFPVFNLYIGGEFEGTVV
                     DADAPIGGERPLKPLQRARVKGHPPLATALLTGLMKHGFDPAFSMTMPKPEEGIGHAI
                     MRPVQTLTDLKTPVVPFLMNCYYAPQPTAQRCYKLGQAVRAIVEDFPDGLRVALIGSG
                     GLWHTPSRPGAWLNEEFDETLLGYMKKGDIQAMAAHFDNYRIPGTDTSQDISVAGRFM
                     TGMPGLSGPQGGGTRETCNWIAAAGAVEGTTATVVDRVPVYASPIDTAFAYFNL"
BASE COUNT          940 a         2037 c         2160 g         1158 t
ORIGIN      
        1 ggcctcggct tcaatctcgc gtggccgttc gtgccgctga tggtgcgcgg cctgggcgtg
       61 cgcgagaacc tggagacgtg ggtgggctac atgctgctcg cgttctacct cgtcagcacc
      121 accgcgagcc cgctgtgggg cggcatcgcc gatcactacg gccgcaagct gatggtgctg
      181 cgcgcgatgc tgggcatggg cgtcgcgatg atgctggtgc cgctcgcgcc gactccggtc
      241 tggttcgccg tggcgctgat gctggtcgcg gtgttcaacg gcttcacccc cgcgggcgtg
      301 tcgctgctgg tggcgaacac gccgcccacg cgcatcggca gcgcggtgtc gctcgcgcag
      361 acgggcgggc aggtggggca cgccatgggg cccgtcgtcg gcgcggcgct ggccgcgctg
      421 atcgaccggc agcacgcgct ctactggatc agcggaggat tcatgttgag cggcgggctg
      481 ctggtcgcgc tgctcgtgcg cgaggtgaag cagctcgcgc cggggccctg gcgcccgcgg
      541 ctggtgggca gcctgcgcga tctcctcggg gtgccgcgca tcgcaccgct gttcctgctg
      601 gcgttcctgt tctcgatcat gtggcacggc agcatcacca acatcagcgt gttcatgctg
      661 cagctgctgg aaattcatca gccgggcgcg gatatggcca cggaagcgtc ctgggtgggg
      721 gcggcggcga cggccatggc gctgtccatg atgttctcgc tgccgctgtg ggggcgcgtg
      781 atcgaccgca tcgggccggg ccgcgtgctc gtgttcgccg ccgcggccag cgtcgttacg
      841 cacctgccgt tgctggtgct gcagacgccg ctgcagctgg tgctgtcgcg ggtgttattc
      901 ggcctgacgg cggccggcat gcagacggcc atcttccagc tgctcaggat gcacgcgccg
      961 ccgggcacgg atgcgcgcgc tctctcttac gcgaccgcgt tccagttcct cgcgatgggc
     1021 atggcgccgt tcacggcggg gctgatcggt cccgcgttcg ggctgcgcgc ctacttcgcc
     1081 gtgaccattg cgctcatgct gggcgggctg gtgctgtggc tgcggacggc aagtcgaaag
     1141 ccggaagtcg acagtcgaaa gtaactatcc gtcatgaacg gtgtcttatt gcgagtgaga
     1201 cccgctcact cgagttccag ggacccgtca tgccgcgttc gcgcattgcc acggccggtt
     1261 accgaaggac gcgtgcgcgt ccccccggca tcccgctagt ccggccgctt ttccgcttcg
     1321 gccggcgggc cctcggcaaa gtaggggccg cgccgctgcc ggtccggctc attgcgggtg
     1381 cggccgtcat cctggcgctc ttctttgcgc tgaactggct ggttcaggtg atccgcaaac
     1441 ccgccgaatt gttcttcccg gtgagcggca cgctgcacaa ggccccgtcc gaaacctggc
     1501 ggcagtacgg gccgctgttt cgcgggcatt ccacggcggt catgaccccc gagctgctgg
     1561 ccgcgctcgc ccaagtcgaa ggcgcgggca atcccgtggc ccggacctac tggcggtggc
     1621 gcctgacgtg gaacccgctc gagctgtacc agcctgcgtc gagcgccgtc ggcatgtacc
     1681 agatcacgaa cgcggcattc caggacgcga ggcgctattg catccatgat cacgcggtgg
     1741 ccgaggacgg cccctggaac gactggcgat cgtgctggtt caacagcctc tacactcgcg
     1801 tcatcccgag tcacgccatc gaaatgacct ccgcgtttct cgatcgcagt gtcgcgcgaa
     1861 cgctcgcgcg ccagcggatc gcggcggcaa cgttcccgca acgacaggat ctcgccgccg
     1921 tcattcacct gtgcgggtcc ggcgtgggcg cggtctatgc gcggcgtggc ttccagctca
     1981 ccgctgccgc gcgctgcggc gaccatgacg tcagggccta cctcgcgcga gtcaacgcga
     2041 tgaagcgcta ctttgcgtgg cgcgccgcgg cacgcgaggg acgagggacg agggacgagg
     2101 gatgagggat gagggacgag ggatgacccc ggcgttcgcc gggaattcac acttggctgt
     2161 cgtcggtcat caccggattg tcaggtcggc ctcgccgacc tcgccggtga aaacggagcc
     2221 ggtctcctcg acgctgcccg cgccgacgta gacgccgatc acttcgagcg gctcggcccg
     2281 gctcaggttg tgcatgaagt gctcgacgcc cctggggatg tagtggacgt ggccggcgcg
     2341 cacttcggcg cggtccgggc cggcgcccgc gaggccgcgg ccgcggacga cgtaatagag
     2401 ttcctcgcag cggtcgtggc ggtgcttctt gtgaacggcg ccgggcatga acttcgccca
     2461 gaagagggtg gtggagctgc cgttgtggcg gccgatgggc aggcggaaat cggtgatgct
     2521 ccagccgtcc ttcgcgttca tccgtgaggg cttcacgtcg tcgatgtgga cgtggatgcc
     2581 cgtgctgaac tgcttcaccc gcggcatctc gagatcggcc ctggtcgcgt ggccgcagaa
     2641 ttcgtagccc gtgtccgcga cgctcctggc gccgacgtag aagccgatga ccagaccttc
     2701 ctcgtccttc gtctcgttgt ggaaaaaatg ctccaatccc ctcggcatga gccggcagtg
     2761 gccgggacgg acctgggcgc gctcggttcc ctgcccgacg acgccgtggc ccttcaggta
     2821 gacggcgatc tcgtcgcagt tgcggtgcag gtgcctggcg tgggtcgagc ccggccggaa
     2881 aatcgagtgg aagagggtgg tggagctgcc gtgcttgccc gagatcggca gccggaattc
     2941 ggagatggcc cagccctcgc cctgcttcat gttctcgggc cggacgtcgt cgacgtgcac
     3001 gagcggatac ttgagcgcca tgcgctcaag tatataaagg cgaaaggcga aagtcgaaag
     3061 gcgaaagtga aaaaagccaa gcgaatggcg aaaagaaccc tggctgacgg gttttacttt
     3121 cgactttcga cttcgcgcga tgcggcggcg tagtatcctg cggaaagtcg tgcaccacca
     3181 ttagcctgga agccccccat gccactcaag cgcgcgaaag gctccgccgg cacggttgtc
     3241 gtgctggagc acgtgtcgaa gatcctcgag ggcaatccgc tgggagaccc gcactcgcgc
     3301 aagctcgcgg tgtggctgcc gccgcagtac gacgattcgg gcgggcgccg cttcccggtg
     3361 ctctacgatt tcgtcggctt cggcggctcc gggctctcgc acacgggctg gaaggggttc
     3421 ggctacaacg tgccggagcg cgcggcgcgg ctgatccgcg aaggcaggat ggggccggcc
     3481 atcctcgtct ttccggactg cttcacggcg ctcggcggca accagtacgt gaactcgacc
     3541 gcgaccggca attacgcgga ctacctcacg cgcgagatca tcccgttcgt ggaccgcgaa
     3601 ttccgcacgc tcgctgcccg cgagcaccgc ggcttcttcg gcaagtcgtc cggcggctac
     3661 ggctcgatga tccacggcat gaaatacgcg aagtactggg gcgccatcgc caatcactcg
     3721 ggtgatgcgt acttcgattt cgtctactgg tgcgactggc cgaacacgct gaatgagctg
     3781 gcgaagcacc gcgtgccgaa acgccggccc ggtcgctacg acgcgcgccg cgaggcgggc
     3841 cgcaggggcc tctccggcgg gcgcgacgac gggcgcatcc ggcgcttcct cgagcgcgtg
     3901 tggaagaagg agaaggtgtc gacggcggag ggccactgca tcatgaacct ctgcatggcg
     3961 gccacctatg acccggaccc gaaagcgccg ctcgggtttc gcgtgccggt caacctcgag
     4021 tcgggcgagc cgatcgcgga gcggtggcgg cagtggcgcc ggcacgatcc catccacctc
     4081 gttgaaaaat accgcgccag cctgaggacg ctgcgcggca tctacatcga ctgcggctgg
     4141 cgcgaccagt accacatcca ttacggctcg cgcatcctgt cgcggcggct cgccgcggcc
     4201 ggcatccgcc atacctacga ggaattcgac gacaatcact ccgacatcga ctaccggatg
     4261 gacgtgagcc tgccgttcct ctaccgggcg ctcaagccct gacgggcgcg cgccctaagt
     4321 cgaaaggcga aaggcaaaag gcgaaaacga aagaagcttt gcttaacggc ggttacttcc
     4381 gactttcgcc ttgcttcatc agttcttctt gttcctcagc ttcttcgccc gcttggccgc
     4441 tttcgccagc tgcttcctgg catagttctt gttccgcctt ctcttcagca tctggttgtg
     4501 acccgtctga ccgcgaccgc tcatggaaac ctccgtcaat tgaatggctg tgcgggcgac
     4561 agtttgcccg ctttccgcgt tcttgtcgtg ccttgcagcg ccgtcttgcg ctatttcacg
     4621 cgccgcaacg ccgtgaagct ggactggttg tccatcgggt tcttgcccgg gtccctggcc
     4681 cgccggtcgt cgaggatcac cagcgatttc gggttgtcgc cgttgggacg cagggtgatg
     4741 gtccagaaca cgtccttgcc tttcttgcgc acgacctcga gggcgtagac gtcgtcaggg
     4801 ggcttgccgt agacgtcggc ttcgaactcg ccctgctcgc agtactttgt ctcgcagtaa
     4861 tgccacatgc gcagccaggc gcgcttgccc tcggtgcgga cgatgatgcg agagacccat
     4921 tcggtgccgt aattcgggat gccttccctt tgccaccagc cggcgtaatt ctccaggggg
     4981 cgcgtcgggc ctgcgtgcgg cgccttcggt ccccagcggt ggcgctctat cgcgttgtcc
     5041 atccattcca gcgtgccggt ggcgtacagg ccgatggcga gcacgaaggg ggcgacaccg
     5101 atggaaatgg cggcggcgcg gttcatgctg gactcatggt tgtccggccg ggtcggcgcc
     5161 gccgctacag gttgaagtag gcgaacgcgg tgtcgatggg ggaggcgtag accggcacgc
     5221 ggtcgacgac cgtcgcggtc gtgccttcga ccgcgccggc cgccgcgatc cagttgcagg
     5281 tctcgcgcgt gccgccgccc tgcgggccgg agaggccggg catgccggtc atgaagcggc
     5341 ccgcgacgct gatgtcctgg ctggtgtcgg tcccggggat gcggtagttg tcgaagtggg
     5401 cggccatcgc ctggatatcg cccttcttca tgtagccgag cagcgtctcg tcgaattcct
     5461 cgttcagcca cgcgcccggc cgcgacggcg tgtgccaaag gccgcccgag ccgatcagcg
     5521 ccacgcgcag gccgtccggg aaatcctcga cgatcgcgcg caccgcctgg ccgagcttgt
     5581 agcagcgctg cgcggtcggc tgcggcgcgt agtagcagtt catcaggaac ggcacgaccg
     5641 gcgtcttgag atcggtcagc gtctgcaccg gccgcatgat ggcgtggccg atgccttcct
     5701 ccggctttgg catggtcatg gagaacgcgg ggtcgaagcc gtgtttcatc agcccggtca
     5761 acagcgccgt ggcgagcggg gggtggccct ttacccgcgc ccgctggagc ggtttcaagg
     5821 ggcgttcccc gccgatcggc gcgtccgcgt ccaccacggt gccctcgaac tcgccgccga
     5881 tgtagaggtt gaagaccggg aagttgctga agtcgaagca ctccatctgg tcgtcgccga
     5941 agatcacgat gacgtccggg cgcgcttgcg ccaccttgcg cttcagcgtg gcgaaactgt
     6001 cctggatgcg cgcgtacttt ttccggttgg tctcgagatc gtccattggc acgtcgggcc
     6061 ggatcgggcg ccccttgcgc gcgtcgttcc aggcctccgg ccggcggtag cagaacgggg
     6121 agtgcgaggt ctggaatacg ccgacgatct gcgccacggc tggaagggaa aagtggaaag
     6181 ggaaaagtcg ctgaaacccg tcagttgatc ttaacgccgg tggtcttgat catcttggcc
     6241 cacttcgcga cttcggcttg caggtagcgc cggaagtcgg cgggagagcc ggggt
//
LOCUS       DBNGSG010000368         2255 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4024188, whole genome shotgun sequence.
ACCESSION   DBNGSG010000368 DBNGSG010000000
VERSION     DBNGSG010000368.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4024188"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="AC158Q_09600"
     CDS             complement(<1..374)
                     /locus_tag="AC158Q_09600"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3340071.1"
                     /translation="MGQKAVVSVENVATFPGEARHNGCSKGVMHADDQHETSVYHVKP
                     RGFIPSHLHSCVYDLFIGIKGRVDITYEGQQGSGRVALSAGSFCSMPPGVRHEVRNNS
                     ANDEAIFLLVHAPSAGYDFIPV"
     gene            complement(400..1989)
                     /gene="ggt"
                     /locus_tag="AC158Q_09605"
     CDS             complement(400..1989)
                     /gene="ggt"
                     /locus_tag="AC158Q_09605"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione gamma-glutamate
                     hydrolase [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIR3340072.1"
                     /translation="MKLFDWQFPYPSQRMPILARNVVATSQPLAAQAGLRMLMKGGNA
                     VDAVLATAISLTVLEPTSNGIGSDAFCILWDGKQIHGLNASGRSPAAWTPDRFKGKFI
                     MPHRGWDSVTVPGAVSAWVDLSQKFGKLPFADLFEPAIRYAADGYMVSPTISRLWAKQ
                     APELKDQPGYAQAFMPNGRAPAAGEKFSFPDQAKTLQRIAETKGEAFYQGDLAERIAA
                     WAKQNGGAMTVADLAAHRNDWVEPISLDYRGYRLLEIPPNGQGIAALLALGMLENLDM
                     PAFPVDSADSLHVQIEAMKLAFADIDEYVSDPATMRVKPAQMLDKAYLKERAKLIDKK
                     KASIPAFGKPSGSGTVYLTAADESGMMVSYIQSNYAGFGSGVVVPGTGISLQNRGYGF
                     VLKPGHANVVAPGKRPFHTIIPGFVTKDGRPLMSFGVMGGSMQAQGHAQIMLRMVDYH
                     QNPQAAADAPRWRIDQGLDVNMEPGSTAEVFADLKHRGHIMSEADRWSTDFGRAQLIF
                     KMEDGYFAASERRTDGQAVGF"
BASE COUNT          367 a          709 c          739 g          440 t
ORIGIN      
        1 tcgacgggga tgaaatcgta acccgcgctc ggagcatgga ccaggaggaa aatcgcttcg
       61 tcattggcgc tgttgttgcg cacttcgtgg cggacgccgg gtggcatgct gcagaacgat
      121 ccggccgaga gcgcgacccg gccgcttccc tgctgcccct cgtaggtaat gtccacccgg
      181 cccttgatac cgataaagag atcgtaaacg cagctgtgca ggtggctcgg gatgaagccg
      241 cgcggcttca cgtggtatac gctcgtctca tgctggtcgt ccgcgtgcat gacgcccttg
      301 ctgcaaccgt tgtgtctcgc ttcaccggga aaggtggcga cattttcgac cgacactact
      361 gctttttgac ccatcgtaat cctccgtaat gggtatcctc tagaagccga cggcctgtcc
      421 atccgtacgc cgctcggagg cggcaaagta cccatcctcc atcttgaaga tcaactgcgc
      481 gcgcccgaag tcggtgctcc agcggtcggc ctccgacatg atgtggccgc ggtgcttgag
      541 gtcggcaaaa acctcggcgg tcgaacccgg ctccatgttg acgtccagcc cctggtctat
      601 gcgccaacgc ggggcgtcgg ccgcggcctg cgggttctgg tggtagtcca ccatgcgcag
      661 catgatctgg gcgtggccct gcgcctgcat cgagccgccc atcacgccga agctcatgag
      721 cggccggccg tctttcgtga cgaagccggg gatgatcgta tggaacggac gttttcccgg
      781 cgcgacgacg ttggcgtggc cgggcttcag gacgaaaccg tagccgcggt tctgcaggct
      841 gatgccggtg ccgggcacga ccacaccgga gccgaaaccg gcgtaattgg actggatgta
      901 cgacaccatc atgcccgact cgtcggccgc ggtgagatag accgtgccgc tccccgacgg
      961 cttgccgaag gcgggaatgc tggctttctt cttgtcgatg agcttcgcgc gctccttgag
     1021 gtaggccttg tcgagcattt gcgcgggttt cacgcgcatc gtcgccgggt cggaaacgta
     1081 ttcgtcgatg tccgcgaagg cgagcttcat cgcctcgatt tgcacgtgca ggctgtcggc
     1141 ggaatccacc ggaaacgccg gcatgtcgag gttctcgagc atgccgagcg cgagcagcgc
     1201 ggcaatgccc tggccgttgg ggggaatctc caggagccgg tagccgcggt agtccaggct
     1261 gatcggctcg acccagtcgt tccggtgcgc ggcgagatcg gcgaccgtca tcgcgccgcc
     1321 gttctgtttc gcccaggcgg caattctttc cgcgagatcg ccctggtaga acgcctcgcc
     1381 cttcgtctcg gctatccgct gcagagtctt cgcctggtcg gggaaggaga atttttctcc
     1441 cgctgccggc gcccggccgt tgggcatgaa tgcctgcgca tagcccggct ggtccttcag
     1501 ttccggcgcc tgcttcgccc acaagcgcga aatcgtcggc gacaccatgt agccgtcggc
     1561 ggcgtagcgg atcgccggct cgaacaggtc ggcgaacggc agcttgccga atttctgcga
     1621 caggtccacc caggccgaca ccgcgcccgg caccgtgacc gagtcccagc cccggtgcgg
     1681 catgatgaac ttgcccttga agcggtccgg cgtccacgcg gccggcgacc ggccggaggc
     1741 gttcaggccg tgtatctgct tgccgtccca caggatgcag aaggcgtcgg atccgatgcc
     1801 gttgctggtg ggctcgagga cggtgagcga gatcgcggtg gcgagcacgg cgtccaccgc
     1861 gttgccgcct ttcatcagca tgcgcagccc cgcctgcgct gcgagcggct gcgacgtcgc
     1921 aaccacgttg cgcgccagga tcggcatgcg ctgcgagggg tacgggaact gccagtcgaa
     1981 gagtttcatg tcgtcaattg cgtgaatagt gaatggtgaa tagtgagtag acgccttgca
     2041 aagggcggtg atgggggtga gcggaggggg ttcagtccaa tcaccattca ctactcacca
     2101 ttcacgcgtt tattactgcg ggacgattcc cgcctgctgc gccaccttgc gccacaaagc
     2161 gacctgggac ttgatggagg cggcgtgctc gtcgatcgag ctgccgatcg gttccgcgcc
     2221 gcggtcgatc agggacttgc tgttcttcgg gtcct
//
LOCUS       DBNGSG010000369         2207 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1138094, whole genome shotgun sequence.
ACCESSION   DBNGSG010000369 DBNGSG010000000
VERSION     DBNGSG010000369.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1138094"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            482..976
                     /locus_tag="AC158Q_09610"
     CDS             482..976
                     /locus_tag="AC158Q_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340073.1"
                     /translation="MAKSPLPRACQPCTACCDGWLQIKINGVPVFPGRPCPHSTGTGC
                     NDYEHRPADPCIHFECGWRRDGSPLPDWMKPDNAKVIVLFDQSRWQGLPVDIAVPVGK
                     RIPPRALNYLKQFAETHQRALLYSEQIVENGVYTNRQVVSAYGPPAFQQEMAQRAGRG
                     EKFI"
     gene            complement(1054..2055)
                     /locus_tag="AC158Q_09615"
     CDS             complement(1054..2055)
                     /locus_tag="AC158Q_09615"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3340074.1"
                     /translation="MQKRKLGKNGPEVSAVGLGCMGMSISYGEPNDEESIATMHRALE
                     LGVNLLVTSDAYGNGVNEELVARAIKGRRDKVLIATKFGNLALASRGADGGGPALTGG
                     HPDYVPQACEKSLKRLGVDTIDIYGMHRMDKAVPIEDTVGAMKRLVEQGKVRYLQLSE
                     AGAQTIRRAHKVHPIACVETEYSLWSRDVEKDILPACRELGIGYMAYAPLGRGFLTAT
                     MKTLDALLPKDRRREHPRFSPDNIAKNAELLAPLERIAAAKQRTPAQIALAWLLAQGA
                     DIVPIPGTKRRTYLEQNCAAADIPLSREEIDSLAKAFPLNVTAGTRYPEKQLSGLGI"
BASE COUNT          394 a          707 c          675 g          431 t
ORIGIN      
        1 tcgtcacggc tgcgcagtgg ggaagagccg tggaatcgag gtcaaggctg ttgaaatcgg
       61 aagcatggtc ctgtgcatcg gtacaccact caaatgcaaa ccctgcggtc accttgcgcc
      121 ggaaaacatg ttaataaagt tatttattat caattagtta tgatttcatc ccggcggtcg
      181 gttggctgat acctggccgg cttcgtaaat ctgcggccag gccaaccccg gtaatacggc
      241 cgaacgggtg cggcaacctg ccgaaaagca cacccggaga ggctggcctt cccccggcac
      301 ttgttacttc gacttgagcc cctgacggct gtccgcccgt tcttcatccg ccgcgcagaa
      361 gcagggccga ttgttccact gtcccgaagc gggatcgatg gcaaaggcgg agcaaatcga
      421 attgaattgc cggtagcgat gtgattaagt gactccacgt tcaccggttt ccggagcctg
      481 cttggcgaaa tccccccttc cccgcgcatg ccagccgtgc acggcttgct gcgacggatg
      541 gctgcagatc aagatcaacg gcgtcccggt attcccggga cggccctgcc cgcacagcac
      601 gggcacggga tgtaacgact acgagcatcg ccccgccgac ccgtgcatcc atttcgagtg
      661 cggctggcgg cgggatggca gcccgctccc ggactggatg aagcccgata acgcgaaggt
      721 gatcgtcctg ttcgaccaga gcaggtggca aggcctgccg gtcgacatcg ccgtaccggt
      781 gggaaagcgg atcccgccgc gcgcgctcaa ttacctgaag cagtttgccg agacgcacca
      841 gcgcgcgctc ctctactcgg agcaaatcgt ggaaaacggc gtctacacca accgccaggt
      901 ggtctcggcc tacgggccgc cggccttcca gcaggaaatg gcgcagcgcg cgggccgtgg
      961 cgagaagttc atctagcctg cttcacaaat gaccaacccc gcatcaatcc cctccttttc
     1021 aaggaggggt ggatgcgaag cagacggggt ggtttagata ccgagccccg agagctgctt
     1081 ctcgggataa cgggtgccgg cggtgacgtt gagcggaaat gctttcgcca gcgaatcgat
     1141 ttcttcccgg ctgagcggga tgtcggccgc cgcgcaattc tgctcgagat aggtgcggcg
     1201 cttggtgccg ggaatcggca cgatgtccgc gccctgcgcg agcagccagg cgagtgcgat
     1261 ttgcgccggc gtgcgctgct tcgccgccgc gatccgctcg agcggcgcga gcagctccgc
     1321 gtttttcgcg atgttgtccg ggctgaaacg cgggtgctcc cggcgccggt ccttgggcag
     1381 cagcgcatcg agcgttttca tcgtcgcggt aaggaagccg cgaccgagcg gcgcgtacgc
     1441 catatagccg atgccgagct cgcggcacgc cggcaggatg tccttctcga cgtcgcggct
     1501 ccacagcgag tattcggtct ccacgcacgc aatcgggtgg accttgtgcg cgcgccggat
     1561 ggtctgcgcg ccggcctccg acagctggag gtagcgcacc ttgccctgct ccaccaggcg
     1621 cttcatggcc ccgacggtgt cttcgatcgg gaccgccttg tccatgcggt gcatgccata
     1681 gatatcgatc gtgtccactc ccagccgctt caggcttttc tcgcacgcct gcggcacgta
     1741 gtcggggtgc cctccggtca gcgccggccc gccgccgtct gcgccccggc tcgcgagcgc
     1801 gaggttgccg aacttggtgg cgatcaggac cttgtcgcgc cgccccttga ttgccctggc
     1861 aaccagctcc tcgttcacgc cgttgccgta ggcatcggag gtcacgagca ggttgacgcc
     1921 gagctcgagc gcccggtgca tggtcgcgat cgactcttcg tcattgggct cgccgtagct
     1981 gatggacatg cccatgcagc ccaggccgac cgccgagacc tccggaccgt tctttcccag
     2041 tttgcgcttt tgcattcttt gctcctcgtt agccgccgat caatgtggat taaacttcaa
     2101 aatcatatta gccgccgatg aacgccgaca gacgccgata tatatcgaca agggtattta
     2161 gaattcagaa tccataaacc acccgtttca ccgttgcttt cggcgca
//
LOCUS       DBNGSG010000370         4764 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_969730, whole genome shotgun sequence.
ACCESSION   DBNGSG010000370 DBNGSG010000000
VERSION     DBNGSG010000370.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4764)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4764)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4764
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_969730"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="AC158Q_09620"
     CDS             complement(<1..700)
                     /locus_tag="AC158Q_09620"
                     /inference="COORDINATES: protein motif:HMM:NF023568.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3340075.1"
                     /translation="MEAFLERPDGCKLYYRVDDYTDPWRRAETALFIHGLAESGEAWR
                     GWVPYFARRYRVVRVDLRGFGRSTPMPAEHPWSMDELLDDIEALMRHLGCERVHLLGA
                     KSGGSMALKLAADRPQRVQTLIGVTPPLVGAADVGAWLERIETRGVLDWARHTMQGRL
                     GTAVSKEEVEWWVANLQGKTPLSTLQGYLRWVPGLDIRADVERISCPTLVITTTGSGL
                     RSVDGVKAWVARMKR"
     gene            complement(887..2503)
                     /locus_tag="AC158Q_09625"
     CDS             complement(887..2503)
                     /locus_tag="AC158Q_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014749682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3340076.1"
                     /translation="MASWRKQTFAQALARAARRFPRQEALVIDGRRLTYSELWKSARR
                     VACALRALGVKRGDHVALCMGNSIEWVQCFYGTAAIGAVTVPVNTRFKADELKYCLKQ
                     SDARLLFVADRFLKVDFIEMLREVCPGVNRRLPHPNLPMLEHVVVLGDDVPKATLHWD
                     TFLGLAGKKDGKAPRVEPGDVLLMQYTSGTTSYPKGVMLTHDNMLRNAACVVERFDRR
                     AGYRYYSARVFYHVAGTTLSLLAALVSGACLLSTPHFEPGEALRLMSAEGCTHTSGND
                     TMFLMLLNHPDLGRYPLNLRGGWASAGPEVSRRAIGQLGMKGLCQAYGLSETSPNVCM
                     SRHDDDLEKRVNGWAHILPGIKVVIADLECGKPLPAGKAGEILVRGWSLMKGYYRMPE
                     QTAKAIDAKGWLHTGDLGMKDEDGRLRFITRIKDVFRVGGENVAPSEVEEVLHQHSAV
                     KQAQVIGVPDPRLIEVPAAYVILRPGASTTPEEIMAWSRERMAGFRVPRYVRIVDSFD
                     SIGMTGSAKVQKNKLRAQALIDFGLQDKAA"
     gene            complement(2691..3248)
                     /locus_tag="AC158Q_09630"
     CDS             complement(2691..3248)
                     /locus_tag="AC158Q_09630"
                     /inference="COORDINATES: protein motif:HMM:NF024854.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR3340077.1"
                     /translation="MRAELTGTTVRNARGSGERPRGRLPSRGAAPAHFPDLSRLLTYN
                     LSLLANMLSRGAAARYSREFGVTLMEWRVIGSLALEAPMSLQQISHRFDLDKGQSSRT
                     VAALLERGLVHRSVNGDDRRSLSLKLTPSGWRLYRRIVQSASARSERLLGSLDVGERK
                     AFLRSFGKLMAEARSIYIEEKSRHK"
     gene            complement(3257..4225)
                     /locus_tag="AC158Q_09635"
     CDS             complement(3257..4225)
                     /locus_tag="AC158Q_09635"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3340078.1"
                     /translation="MRKGFMRVILISICSAASAGAQAQAGYPAKPIRLVVPFAPGASN
                     DSISRATADAMSPLLGQPIVVENRPGAGGMIGAENVARAAPDGYSLLNIQVSFVTNAA
                     IREKMPYDVTRDFAYIGMMTKSPMILVVHPSMPVKSVKELVAICRKRPGDLNYGSSGT
                     GGSNHLATELFATAAGIRITHVPYKGIALALTDLMGGHVQMVLTSLPSVLGQVKSGRV
                     RALGVAGDRRSPFAPDIPTVAEGGVQYTSELWWGLAAPGKTPPETVRRLSETLQQALQ
                     TSALGKRYAAEGGEPIRTTPQAFSKFVLEEVEQWRQVAKKSGVTLR"
     gene            4460..>4764
                     /locus_tag="AC158Q_09640"
     CDS             4460..>4764
                     /locus_tag="AC158Q_09640"
                     /inference="COORDINATES: protein motif:HMM:NF013253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HIR3340079.1"
                     /translation="MLNDGMPAAYDVADLRELARRRLPKGVFEFFDRGNGDEVALIEN
                     RAAFERIKLAPHALVDTSRRSQEITLFGRRHRMPIALAPTGSAGLAWHEGEIALARAA
                     "
BASE COUNT          779 a         1585 c         1581 g          819 t
ORIGIN      
        1 cacgcttcat gcgcgccacc caggctttca ccccgtccac cgaacgcagg ccgctgccgg
       61 tggtggtgat gacgagggtg gggcagctga tgcgctcgac gtcggcgcgg atatcgagtc
      121 ccggcaccca gcgcaggtag ccttgcagcg tcgacagcgg cgtcttgccc tggaggttcg
      181 cgacccacca ttcgacttct tccttcgaca ccgcggtgcc gaggcggccc tgcatggtgt
      241 ggcgcgccca gtcgagcacg ccacgggtct cgatgcgctc gagccacgcg ccaacatcgg
      301 cggcgcccac aagaggcggg gtaacgccaa tcagggtctg cacccgctgg ggccggtccg
      361 cggcgagctt caatgccatc gagccgccgc tttttgcgcc gaggaggtga acgcgctcgc
      421 agccgagatg ccgcatgagc gcctcgatat cgtccagcag ttcgtccatg ctccacgggt
      481 gctccgccgg catcggcgtc gagcgcccga agccgcgcag gtccacacgc accacgcggt
      541 aacgtcgcgc gaaatacgga acccagccgc gccaggcctc gccggactcc gcgagcccgt
      601 ggatgaacaa cgcggtctcg gcccgacgcc aggggtccgt gtagtcgtcg acccggtagt
      661 aaagcttgca gccatcggga cgttcgagaa acgcttccac tcaatggtcc tgttcagata
      721 ccggattaac cgccgatgaa cgccgataca cgccgatgag atcaaggatg ccctgcaact
      781 tcgtcatttc cgcgaaggcg gaaatccacg catgacggaa aactggcgtc gggatgcccg
      841 ggtttttatc tgagttcatc ggcggctgtc tggttttggg cgagcgtcag gcggccttgt
      901 cctgcagccc gaaatcgatc aacgcctggg cgcgcagctt gttcttctgc actttggcgc
      961 tgccggtcat gccgatgctg tcgaacgagt ccacgatcct cacgtagcgc ggcacgcgga
     1021 aacccgccat gcgctcgcgg ctccaggcca tgatttcctc cggcgttgtc gatgcgccgg
     1081 gcctgaggat cacgtaggcg gcgggcacct cgatcagccg cgggtcgggc acgccgatca
     1141 cctgcgcctg tttgacggcg gagtgctggt gcagcacctc ctcgacttcg gagggcgcga
     1201 cgttctcgcc gccgacccgg aacacgtcct tgatgcgggt aatgaagcgc aggcgcccat
     1261 cctcgtcctt catgccgaga tcgccggtat ggagccagcc tttcgcgtcg atcgccttcg
     1321 cggtctgctc cggcatcctg taatagccct tcatcaggct ccagccgcgc acgagtattt
     1381 cccccgcctt gccggccggg agcggcttgc cgcactccag gtccgcgatg accaccttga
     1441 tgccggggag aatatgcgcc cagccgttca cgcgcttctc gaggtcgtcg tcgtgccgcg
     1501 acatgcagac attgggcgag gtctcggaca gcccgtaggc ctggcacagg cctttcatgc
     1561 ccagctgccc gatcgccctg cgcgagactt cgggacccgc cgaggcccag ccgccgcgca
     1621 gattcagggg ataacggccg agatcggggt ggttcagcag catcagaaac atcgtgtcgt
     1681 tgcccgaagt atgggtgcag ccctccgcgc tcatcaggcg caacgcctct ccgggctcga
     1741 agtgcggcgt ggaaagcagg cacgcgccgg acaccagcgc cgcgagcagc gacagcgtcg
     1801 tgccggcgac gtggtagaag acgcgcgcgc tgtagtagcg gtagccggcc cggcggtcga
     1861 agcgctcgac gacgcaggcc gcattgcgca gcatgttgtc gtgggtcagc atcacgccct
     1921 tggggtagga cgtggtgccc gaggtgtact gcatcagcag cacgtccccg ggttccaccc
     1981 gtggcgcctt gccgtctttc ttgccggcca ggccgagaaa cgtgtcccag tggagggtcg
     2041 ccttcggcac gtcgtcgccg agcacgacga cgtgctcgag catgggcagg ttggggtgcg
     2101 gcagccggcg gttcaccccg gggcatactt ccctcagcat ttcgatgaaa tcgaccttga
     2161 ggaagcggtc cgcgacgaac agcagccgcg catcggattg cttgaggcag tacttgagct
     2221 catcggcctt gaagcgagtg ttgaccggca ccgtgaccgc gccgatcgcc gccgtgccat
     2281 aaaagcactg cacccactcg atcgagttgc ccatgcagag cgcaacgtgg tcgccgcgct
     2341 tcaccccgag cgcgcgcagg gcgcaggcga cccgccgcgc cgatttccac aactcggagt
     2401 aggtgaggcg ccgcccgtcg atcaccagcg cttcctgccg gggaaagcgg cgcgcggcgc
     2461 gcgcgagcgc ctgggcgaag gtctgttttc tccagctggc catgttgata gacaggattt
     2521 acaaaataat tcaggaaccc gtcaaaacca tcaatagacg gctgccccca tcctaccttc
     2581 ccccgcatgc gggggaaggg gataaccaag atttacagga ttcaaccaaa aacaaggcaa
     2641 gatcgaatta ctgttggtcg ttctaatcct gttaatcatg tacatccttt ctatttgtga
     2701 cgggattttt cctcgatgta gatcgagcgg gcctcggcca tgagcttgcc gaagctgcgc
     2761 aggaaagcct tgcgttcccc gacgtcgagg ctgcccagca gccgctcgct gcgcgcgctg
     2821 gcgctttgca cgatgcggcg gtagagccgc catcccgagg gcgtgagctt cagcgagagg
     2881 ctgcggcggt cgtcgccgtt cacgctgcgg tggaccaggc cgcgctcgag cagggcggcc
     2941 accgtgcggc tcgactggcc cttgtcgagg tcgaaacggt gcgagatctg ctgcagcgac
     3001 atcggcgctt cgagcgcgag gctgccgatc acccgccatt ccatgagcgt gacgccgaac
     3061 tcgcgcgaat agcgcgccgc cgcgccgcgg ctcagcatgt tggcgagcag gctcaggttg
     3121 taggtgagca gccgggacag gtcgggaaaa tgagcgggcg ccgcgccgcg ggacgggagc
     3181 ctgccgcggg ggcgctctcc gctgccgcgc gcattccgga ccgtcgtgcc tgtcagttcc
     3241 gcgcgcatcg gcggctctac ctgagcgtca cgcccgattt tttcgcgact tgccgccatt
     3301 gctcgacttc ctcgagcacg aatttcgaaa aggcctgcgg cgtcgtccgg atcggttcgc
     3361 cgccttccgc ggcgtagcgc ttgccgagag cggaggtctg cagtgcctgc tggagcgtct
     3421 ccgagagcct gcgcacggtc tcgggcggtg tcttgcccgg cgcggcgagg ccccaccaga
     3481 gctcgctcgt gtactgcacg ccaccctccg ctacggtggg aatatccggc gcgaagggag
     3541 aacgcctgtc gcccgcaaca ccgagcgccc gcacccggcc ggatttcacc tgccccagca
     3601 ccgagggcag gctcgtcagg accatctgta cgtgtccgcc catgagatcg gtcagggcga
     3661 gcgcaatgcc cttgtagggc acgtgcgtaa tcctgattcc ggccgcggtc gcgaacagct
     3721 cggtcgccag gtgattgctg ccgcccgtgc cggacgagcc gtagttcagg tcgcccggcc
     3781 gcttgcggca aatcgccacg agttctttca ccgacttgac cggcatggag gggtgaacga
     3841 ccaggatcat cgggctcttg gtcatcatcc cgatataggc gaagtcccgg gtgacgtcgt
     3901 agggcatctt ttcgcggatc gccgcattgg tcacgaacga cacctggatg ttgagcaggg
     3961 aatacccgtc gggtgccgcg cgcgcgacgt tctccgcgcc gatcatgccc cccgcgccgg
     4021 ggcggttttc gaccacgatc ggctggccga gcagcggcga catggcgtcc gccgtcgcgc
     4081 gggagatgct gtcgttactc gcgcccggtg cgaacggcac caccaggcgg atgggcttgg
     4141 cgggatagcc ggcttgtgcc tgcgcgcccg cgcttgcggc ggagcagatc gagatcagga
     4201 tgacgcgcat gaagcccttg cgcatgatgg ctgctccctc ttggcgtttt ccgaatgggc
     4261 ctgcgcgatc ggcggcccgg ccctttgcgc gccgcgcgag ccgccgcggc acgctcctcc
     4321 ccgggaaact taggccagtt ggttgatggt gtcaaccaat caggcggggt cggccgcgac
     4381 cgattccgca ttgtgtcagc gccgggcggt ccggggcgtc acgatatact gggcggcccc
     4441 gcaatatcgc gagaaatcaa tgctgaacga tggaatgccg gccgcctacg acgtcgcaga
     4501 cctgcgcgag ctggcgcggc gccggctgcc gaagggggtc ttcgagttct tcgaccgcgg
     4561 caacggcgac gaggtggcgc tcatcgagaa ccgggctgcg ttcgagcgca tcaagctcgc
     4621 tccccacgcg ctggtggaca cgtcccgccg cagccaggag atcacgctgt tcggccggcg
     4681 gcaccggatg ccgatcgcgc tcgcgccgac cgggtcggcc ggactggcgt ggcacgaggg
     4741 cgagatcgcg ctggcgcggg cggc
//
LOCUS       DBNGSG010000371         3412 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2328214, whole genome shotgun sequence.
ACCESSION   DBNGSG010000371 DBNGSG010000000
VERSION     DBNGSG010000371.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2328214"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="AC158Q_09645"
     CDS             <1..384
                     /locus_tag="AC158Q_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006408353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIR3340080.1"
                     /translation="IEHAERMGLAQLHQLRGRIGRGADASTCILLYQNPLTPAARERL
                     KVIYEQTDGFEIARHDLKLRGPGEILGARQSGTPMLRFADFETDLDLLDAARTAAETL
                     LREQPDCARAHLARWLGARHELLKA"
     gene            538..1428
                     /gene="ubiA"
                     /locus_tag="AC158Q_09650"
     CDS             538..1428
                     /gene="ubiA"
                     /locus_tag="AC158Q_09650"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     polyprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HIR3340081.1"
                     /translation="MTLTEKLDAYERLMRLDKPIGILLLLWPTLWALWLAAPGLLRLD
                     VLIIFLVGTVLMRSAGCVINDYADRNFDARVERTRDRPLATGQVKPAEALVLAAVLLL
                     LAFVLVLRLHRLAIYLAFVGVAVTVIYPFLKRFFAFPQAWLGIAFSFGIPMAYAAQRG
                     TVPAAAWVMMAATLFWIIAYDTEYAMVDRDDDAKIGLHSSAILLGRHDVAAVMACHGV
                     FLAIMTGIGLWYRLGAYYYLGLALAAAVVAYQYWLIRARERERCFRAFLSNNWVGMAI
                     FAGLLLDLQLGGRVYQGFTQ"
     gene            1425..2897
                     /gene="ubiD"
                     /locus_tag="AC158Q_09655"
     CDS             1425..2897
                     /gene="ubiD"
                     /locus_tag="AC158Q_09655"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041322210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HIR3340082.1"
                     /translation="MKYRDLRDFLAQLESRSELKRITATVDPRFEMTEICDRVLKAGG
                     PALMFEKPRGHAIPVLGNLFGTVRRVALGMGVDAPDPTAALRELGRLLATLKEPEPPR
                     GWKDAWDKLPLLKQVLSMAPRTVSGAPCQEIVHEGPDVDLARLPIQTCWPGDAAPLIT
                     WGLTVTRGPNQRRQNLGIYRQQVIDRNKVIVRWLAHRGGALDYRDHGLAHPRDPFPVA
                     VALGADPATILGAVTPVPDALSEYQFAGLLRGSRTEVVKCIGSDLLVPAGAEIVLEGR
                     IHPGETALEGPFGDHTGYYNEQDRFPVLTIDRITTRRDPIYHSTYTGKPPDEPAVLGM
                     ALNEVFVPLLQRQFPEIVDFYLPPEGCSYRIACVSMKKQYPGHAKRVMFGIWSFLRQF
                     LYTKFIIVTDADVSVRDWKEVVWALATRVDPKRDTLITEHTPIDYLDFASPVPGLGSK
                     MGIDATHKWPGETDRRWGEAIAMSEEVKRRVDALWRDLGL"
     gene            complement(2899..>3412)
                     /locus_tag="AC158Q_09660"
     CDS             complement(2899..>3412)
                     /locus_tag="AC158Q_09660"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HIR3340083.1"
                     /translation="KFSNNVMARQLYLTLGAEGPGAPATPEKSARVIRQWLAGKSLPV
                     PELVLENGSGLSRAERVSARNLGLLLVAAYRSPVMPELIASLPVVAVDGTMRKRLAGA
                     NVAGQAHIKTGSLAGVRSIAGYVLDGQGRRMVVVLFVNHTNAGNTQAVQDALLRWIYS
                     READGCCPRR"
BASE COUNT          541 a         1148 c         1141 g          582 t
ORIGIN      
        1 atcgagcacg cggagcgcat ggggctcgcg caactgcacc agttgcgggg tcgcatcggg
       61 cgcggggcgg acgccagcac ctgcatactg ctgtaccaga acccgctcac gccggcggcg
      121 cgcgagcggc ttaaagtgat ctacgagcag accgacgggt tcgagatcgc gcgccacgat
      181 ttgaagctgc gcggcccggg ggagatcctc ggcgcgcgcc agagcggcac cccgatgctg
      241 cgcttcgccg atttcgaaac ggatctcgac ctgcttgatg ccgcgcgcac cgccgccgaa
      301 acgctgttac gcgagcagcc cgactgcgcg cgcgcgcatc tcgcgcgctg gctgggcgca
      361 cgccacgaac tgctgaaagc gtgattcgcg aatggtgaat cgtgaatcga agaagatagc
      421 gaagccaggc atgctggttt aatcgaatca cgactcacga atcacgattc acggggtgtc
      481 ctaatcgcct atactggtga ccgaaatatt cctgcccggc gtactgaagc tcggaccatg
      541 acgctcacgg aaaagctcga tgcctacgag cggctgatgc ggctcgacaa gccgatcggc
      601 atcctgctgc tgctgtggcc cacgctgtgg gccctgtggc tggcggcgcc cggcctgctg
      661 cggctcgatg tgctgatcat attcctggtg ggcacggtgc tgatgcgttc cgccgggtgc
      721 gtcatcaatg attacgccga ccgcaatttc gatgcgcggg tggagcgcac ccgtgaccgc
      781 ccgctcgcca ccggccaggt gaagccggcc gaggcgctgg tgctcgcggc cgtgctgttg
      841 ctgctcgctt tcgtgctcgt gctgcggctc caccggctcg ccatctacct cgcttttgtc
      901 ggagttgcgg tgacggtcat ctatcctttc ctcaagcggt tcttcgcgtt cccccaggcg
      961 tggctcggca tcgcgttcag tttcggcatt cccatggcct acgccgccca gcgcggcacc
     1021 gtgccggcgg cggcctgggt gatgatggcc gcgacgctgt tctggatcat cgcgtatgac
     1081 acggagtacg cgatggtgga ccgcgacgac gacgcgaaga tcggcctgca ctcctccgcc
     1141 atcctgctcg gccgccacga cgtggcggcg gtgatggcct gccacggcgt tttcctcgcg
     1201 atcatgaccg ggatcgggct gtggtaccgc ctgggcgcgt actactatct cggcctcgcg
     1261 ctggctgccg ccgtggtggc gtaccagtac tggctcatcc gcgcgcgcga gcgcgagcgc
     1321 tgcttcaggg cgttcctcag caataactgg gtcggcatgg cgatcttcgc cgggctgctg
     1381 ctcgacctgc agctgggcgg acgcgtgtat caggggttta cgcaatgaaa taccgcgacc
     1441 tgcgcgattt cctggcccag cttgaatcgc gcagcgaact gaaacgcatc accgcgacgg
     1501 tcgatccccg tttcgagatg accgagattt gcgaccgcgt gctgaaagcg ggcgggccgg
     1561 cgctcatgtt cgagaagccc cggggacacg cgatcccggt gctcggcaac ctgttcggca
     1621 cagtgaggcg cgtcgcgctc ggcatgggcg tggatgcccc ggatcccacc gcagcgctgc
     1681 gcgaactcgg ccgattgctg gcgacgctca aggagcccga gccgcccagg ggctggaagg
     1741 atgcctggga caagctgccg ctcctgaagc aggtgctgtc gatggcgccc cggaccgtgt
     1801 ccggcgcgcc ttgccaggag atcgtgcacg aaggccccga cgtggacctc gcgcgattgc
     1861 cgatccagac ctgctggccc ggggacgccg cgccgctcat tacctggggc ctgacggtca
     1921 cgcgcggccc caaccagcgc cggcagaacc tcggcattta tcgccagcag gtgatcgatc
     1981 gcaacaaggt catcgtgcgc tggcttgccc accgcggcgg cgcgctcgac taccgcgatc
     2041 atggcctcgc gcacccgcgc gatcccttcc cggtggcggt ggcgctggga gcggacccgg
     2101 ccacgatcct gggcgcggtc accccggtgc cggacgcgct ctccgagtat caattcgccg
     2161 gcttgctgcg cgggtcgagg acggaagtcg tcaaatgcat cggcagcgac ctgctggtgc
     2221 cggctggcgc ggagatcgtg ctcgagggcc ggattcatcc cggcgagacc gcgctcgagg
     2281 ggccgtttgg cgatcacacc ggctactaca acgagcagga tcgcttcccg gtgctgacga
     2341 tcgaccggat cacgacccgg cgcgacccga tttaccattc gacctatacg gggaagccgc
     2401 ccgacgagcc ggcggtgctg ggcatggcgt taaacgaagt gttcgtgccg ctgcttcagc
     2461 gccagttccc ggagatcgtg gatttctacc tgccgcccga aggctgctcc taccgcatcg
     2521 cctgcgtcag catgaagaag cagtacccgg ggcacgccaa gcgcgtgatg ttcggcatct
     2581 ggtcgttcct gcgccagttc ctctacacca agttcatcat cgtcaccgac gccgatgtca
     2641 gcgtgcgcga ctggaaggaa gtggtctggg cgctggcgac gcgagtggac ccgaagcgcg
     2701 acacgctcat caccgagcac acgccgatcg actacctcga tttcgcgagc cccgtgccgg
     2761 ggctgggctc caagatgggg atagacgcca cccacaagtg gccgggtgaa accgaccgcc
     2821 gctggggcga ggcgatcgcg atgagcgagg aagtgaagcg ccgcgtggac gcgctgtggc
     2881 gagatctggg gctctgattc agcgccgcgg gcagcagccg tcggcctcgc ggctgtaaat
     2941 ccaccgcagc agcgcatcct gcaccgcctg cgtgttgccc gcgttcgtgt ggttgacgaa
     3001 taggaccacg accatgcgcc gtccctggcc gtcgagcaca tagcccgcga tcgagcgcac
     3061 gcccgcgagg gacccggtct tgatgtgcgc ctgtcccgcg acattcgcgc ccgccagccg
     3121 ctttctcatg gtgccgtcga ccgccacgac cggcagcgag gcgatcagct ccggcatcac
     3181 cgggctgcgg taggctgcga ccagcagcag cccgagattg cgcgcgctga cgcgctccgc
     3241 gcgcgagagt cccgagccgt tctccagcac gagttcgggg accggcaggc tcttgcccgc
     3301 cagccactgg cggatgacgc gggccgactt ctcaggggtc gccggggctc ccggtccctc
     3361 cgcgcccagc gtcaggtaga gctggcgcgc catcacgttg ttggagaact tg
//
LOCUS       DBNGSG010000372         5769 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_800459, whole genome shotgun sequence.
ACCESSION   DBNGSG010000372 DBNGSG010000000
VERSION     DBNGSG010000372.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5769)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5769)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5769
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_800459"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2576)
                     /locus_tag="AC158Q_09665"
     CDS             complement(<1..2576)
                     /locus_tag="AC158Q_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HIR3340084.1"
                     /translation="MKLNDTGLALPPQQVTLDVLLEKYAKGSEKNVGDVRRRVARALA
                     AVEKDPARWEPVFFEALECGFIPGGRVNSAAGTALQATLINCFVQPVGDSVSETVDGK
                     PGIYVALMEAAETMRRGGGVGYDFSSIRPKGARVRGTDSTASGPLSYMRVFDRSCETV
                     ESAGSRRGAQMGILRCDHPDIEDFIHAKDQGDLKNFNVSVAVTDAFMRAVDADGDWDL
                     VHKQPPAPDFEGESRQRADGLWVYRKVKARDLWQQVMESTYDHAEPGVFFVDRANADD
                     NLSYVEKIEATNPCVTADTWVMTGDGPRQVGELVGRPFTAIVNGKGYPTQSPGFFATG
                     RKRVLKLSTACGLALRLTGNHLVRRVSRRTRYVIETSWMAASELKPGDEIVLHDHRTH
                     TGWSGAGTEAEGYLIGTLIGDGVLMKDKAVLSVWDPQMRRTVHGESVMSSGAASMMQA
                     IEAAARTLPHRSDFGGWQKIIAGRVEFRLAMAALRNLAGSLGLRPNHKRLTTEIEHCS
                     SDFYSGVLRGLFDTDGSVQGTQAKGVSVRLTQVDLENLLRAQRMLLRLGIVSTIYEDR
                     KPAGTKSLPDGKGSTRLYPTQAVHELVISGENLLRFEQSVGFEHSEKADRLRILLSSY
                     RRQLNHERFVDTVDAIVDDGVEEVFDVTVSEVSAFDANGIYVHNCGEQPLPPYGCCCL
                     GSVDLTPFVREPFGARAHFDFEAFGKVVRTATRMLDNVLDATVWPLKQQHEEARAKRR
                     VGLGFTGLGDALIMLGLRYDTDDARRCAARIMEAMRDEAYTASIEIAREKGAFPLLDA
                     ERYLAAPHCASRLPEKLKSDIRTHGIRNSHLLSIAPTGTISLAFADNASNGIEPAYSW
                     TY"
     gene            complement(2758..4332)
                     /locus_tag="AC158Q_09670"
     CDS             complement(2758..4332)
                     /locus_tag="AC158Q_09670"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3340085.1"
                     /translation="MEGSRLPGPICQEKVITTSFESLGLSAELLRAVAEQGYTEPTPI
                     QAQAIPVVLAGRDLLGAAQTGTGKTAGFTLPLLQRLAAGGNASASPTRHPVGALILVP
                     TRELAAQVKDSVRAYGRFLKLRATVIFGGVGFNPQADELRRGVDIVVATPGRLLDHVQ
                     QRTIDLRQVEILVLDEADRMLDMGFIPDIRRILALLPLRRQNLLFSATFPDEIRKLAG
                     SFMRDPATVEVAPRNKPVELISQIAHPVDSGRKRELLSHLVKTNNWQQVLVFTRTKHG
                     ANRLAQQLERDGIDADAIHGNKSQGARTRALKRFKDNELQVLVATDIAARGLDIEELP
                     HVVVYDLPHVAEDYVHRIGRTGRAGVSGEAVVLVSGEDRPLLAAVERLIGRRIEQRVI
                     AGFETGTASHAPAQRHEPQRHERGRQGAGQSHRRNSSGNGNGGNRQQQNSRQERRNEP
                     RSPGQGPQSQPRHGQRKPQRPHQHPQSQQPAHARQPSHAGEGEDRQPPPERAGNQLFP
                     GLLGGLGRRMFGRKAG"
     gene            complement(4593..>5769)
                     /gene="mnmC1"
                     /locus_tag="AC158Q_09675"
     CDS             complement(4593..>5769)
                     /gene="mnmC1"
                     /locus_tag="AC158Q_09675"
                     /inference="COORDINATES: protein motif:HMM:TIGR03197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC1"
                     /protein_id="HIR3340086.1"
                     /translation="LAGAAACERLAARGWRVELIERHAAPAAEASGIAAGVFHPHVSR
                     DDSLLSRLTRAGFLYATANWRALERNGHRFAWARCGVLQIARDAREEARFAAAVRALG
                     PLPDYVDFAPRGEAGRLAALAVTGGGLWFPGSGWMRPAELASAQIAAAAARGNLKLRL
                     GVAVHALDRAGNGWRILSADGTAIASAPVVVLANSNDAGRLAATGVALKRVRGQLTCL
                     PAGSLAGPGAVLAGAGHLIPLADGSAIAGATYDFEDESAEPREEDHAGNLERLERLLP
                     GTAARLDPSRLTGSVGFRCVAPDRLPLIGSMPEAGAQPGARAGILPRLPGLYGAFAYA
                     SRGLTWAALGGELIASAIEGEPLPVESDLADAIDPARFMLRRARRAQASAGGKNKQD"
BASE COUNT          920 a         1918 c         1915 g         1016 t
ORIGIN      
        1 tggtaggtcc acgagtacgc gggctcgatg ccgttggagg cgttgtcggc gaacgcgagc
       61 gagatcgtgc cggtgggcgc gatcgagagc aggtggctgt tgcggatgcc gtgcgtgcgg
      121 atatcgctct tcagcttctc cggcaggcgc gaggcgcaat gcggagcggc aaggtatcgc
      181 tccgcatcaa ggagcgggaa agctcctttt tccctggcaa tctcgatgga ggcggtatag
      241 gcttcatcgc gcatggcttc cataatgcgc gccgcgcagc ggcgcgcatc gtccgtgtca
      301 taacgcaggc cgagcatgat cagcgcgtcg cccagcccgg tgaagccgag gccgacgcgg
      361 cgcttggcgc gcgcctcctc gtgctgctgc ttcagcggcc acacggtggc gtcgagcacg
      421 ttgtcgagca tgcgcgtcgc ggtgcgcacg accttcccga aggcttcgaa gtcaaagtgc
      481 gctcttgcac cgaacggctc gcgcacgaac ggagtcaaat cgaccgaacc gaggcagcag
      541 cagccgtaag ggggaagcgg ctgctcgcca caattgtgta cgtagatgcc gttggcgtcg
      601 aacgcactga cctcactcac ggtgacgtcg aatacctcct caacaccatc gtcgacgatc
      661 gcatccaccg tgtcgacaaa acgctcatga ttgagttggc gccggtatga cgacaggaga
      721 atccttagcc tgtccgcttt ttccgaatgc tcgaatccga cagactgctc gaaccgcagg
      781 aggttctccc cgctgatcac aagttcatgc accgcctgcg tggggtagag ccgcgtgctg
      841 cctttgccat cgggcagcga tttggtccct gcgggctttc tatcctcata gatggttgag
      901 acaatgccga gccgcagaag catccgttgg gctcgcagca ggttttccag atcgacctgg
      961 gtcaatcgga cgctgacacc tttggcctgc gtgccttgca ccgagccgtc agtatcgaaa
     1021 agtccgcgca atacaccgct atagaagtcc gatgagcaat gctcgatctc ggtcgtcagc
     1081 cttttatggt tgggacgcag ccccagtgag ccggcgaggt ttcgcagcgc ggccatcgca
     1141 agcctgaact cgactcgtcc agcaattatt ttctgccagc cgccgaaatc gcttcggtga
     1201 ggcaaagtcc gtgctgccgc ctcgatcgcc tgcatcatgc tggccgcgcc cgaagacatt
     1261 acgctttcgc cgtgcacggt tcgccgcatc tgtgggtccc agaccgacag cacggcttta
     1321 tctttcatga gaaccccatc gccgatcagc gtgccaatga ggtaaccctc cgcttccgtg
     1381 ccagccccgc tccagccagt gtgagtgcgg tgatcgtgca ggacgatctc gtcgcccggc
     1441 ttgagttcgg atgcggccat ccaggatgtc tcgatcacgt accgggttcg gcgagagact
     1501 cgtcggacta gatgattgcc tgtcaggcgc aaggctaggc cacacgcggt gctgagcttc
     1561 aagacacgtt tgcggcccgt tgcaaagaac cccggtgact gggtgggata gcctttgccg
     1621 ttgacaattg cggtgaacgg ccggcccacc agttcgccaa cttgccgcgg cccgtcgccg
     1681 gtcattaccc aggtgtcagc ggtgacacag gggttggtcg cctcaatttt ttcgacgtaa
     1741 gaaagattgt catcggcatt cgcgcggtct acgaagaaca cacccggctc ggcatggtcg
     1801 taggtggact ccatgacctg ctgccagaga tcgcgcgcct tcaccttccg atagacccac
     1861 aggccgtcgg cgcgctgacg cgattcgccc tcgaaatccg gcgcgggcgg ctgcttgtgc
     1921 acgaggtccc agtcgccgtc ggcatcgacc gcgcgcatga aggcgtcggt caccgccacg
     1981 ctcacgttga agttcttgag gtcgccctgg tccttggcgt ggatgaaatc ctcgatgtcg
     2041 ggatggtcgc agcgcaggat ccccatctgc gcgccgcggc gcgagccggc ggattccacc
     2101 gtctcgcagc tgcggtcgaa cacgcgcatg tacgatagcg gcccgctcgc ggtgctgtcg
     2161 gtgccgcgca cccgcgcgcc cttcggccgg atcgatgaaa aatcgtagcc cacgccgccg
     2221 ccgcggcgca tcgtctccgc cgcttccatc agcgcgacgt agatgccagg cttgccgtcg
     2281 accgtctccg ataccgagtc gccgactggc tgcacgaaac agttgatgag cgtcgcctgc
     2341 aacgccgtgc cggccgcgga attgacccgc ccgccgggga tgaaaccgca ctcgagcgct
     2401 tcgaaaaaca ccggctccca gcgcgcgggg tccttttcca ccgccgccag cgcgcgcgcg
     2461 acgcgccggc gcacgtcccc gacgttcttt tcggagccct tggcatactt ctccagcagc
     2521 acgtcgagcg tgacctgctg cgggggcagg gcgaggccgg tatcgttgag cttcatgtca
     2581 ggttggagcg ggtccggttg tcccggttcc gggccgaaaa agggaatgat accgtccgcg
     2641 ggcggtggaa ttaagcgccg gatggcgtcc gcttgatgaa aatcaaaaac ggcgggtttt
     2701 ccgccgtttt cgtccatcga gcctgcctgc cggcgcgggc ggacgcccgc gccgtgatta
     2761 accggccttg cggccgaaca tcctgcggcc cagcccgccc agcaatcctg gaaacaactg
     2821 gtttccggcg cgctccggcg ggggttgccg gtcctcgccc tctccggcgt gcgacggctg
     2881 ccgcgcatgc gcgggttgct ggctttgcgg gtgctgatgc gggcgctgcg gcttgcgctg
     2941 cccgtgacgc ggctgcgact gaggtccctg cccgggagag cgcggctcgt tgcgccgttc
     3001 ctgccggctg ttctgctgct ggcggttgcc gccgttgccg ttgccgctgc tgttacggcg
     3061 gtgcgactgc cctgcaccct gccgtccgcg ctcgtggcgc tgcggctcgt gccgctgcgc
     3121 cggagcgtgg gatgctgttc ccgtttcgaa tcccgcgatg acccgctgct cgatgcgccg
     3181 gccgatcagg cgctcgaccg cggccagcag cggccggtct tcgccgctca ccagcacgac
     3241 tgcctcgccc gaaacacccg cgcggccggt gcggccgatg cggtgcacgt aatcctcggc
     3301 gacatgcggc aggtcgtaga ccaccacgtg cggcagttcc tcgatgtcga gcccgcgcgc
     3361 cgcgatgtcg gtcgcgacca gcacctgcag ctcgttgtcc ttgaagcgct tgagcgcccg
     3421 cgtgcgcgcc ccctggctct tgttgccgtg gatcgcgtcg gcatcgatgc cgtcgcgctc
     3481 gagctgctgg gcgagccggt tggcgccgtg cttggtgcgg gtgaatacca gtacctgctg
     3541 ccagttgttg gttttcacga ggtgcgagag cagctcgcgc ttgcgccccg aatccaccgg
     3601 gtgcgcgatc tgcgagatga gctcgaccgg cttgttgcgc ggcgcgacct cgacggtcgc
     3661 agggtcgcgc atgaacgagc cggcgagctt gcggatctcg tcgggaaaag tcgccgagaa
     3721 cagcaggttc tggcgcctca aaggcagcag cgcgaggatg cggcggatgt cggggatgaa
     3781 gcccatgtcg agcatgcggt cggcctcgtc cagcaccagg atctccacct ggcgaagatc
     3841 gatcgtgcgc tgctgcacgt ggtcgaggag ccgccccggc gtcgcgacga cgatgtcgac
     3901 gccccggcgc agttcatcgg cctgcgggtt gaagccgacg ccgccgaaga tgacggtcgc
     3961 gcgcagcttg aggaagcggc cgtaggcgcg cacgctgtcc ttcacctgcg ccgcgagttc
     4021 gcgggtcggc accaggatca gcgccccgac cggatggcgc gtgggtgaag cgctggcgtt
     4081 gccgccggcc gcgagccgct gcagcagagg cagggtgaag ccggcggtct tgccggtgcc
     4141 ggtttgcgcg gcgcccagca ggtcgcgccc cgcgagcacg acgggaatgg cttgcgcctg
     4201 gatgggggtg ggttcggtgt aaccctgctc ggcgacagcg cggagcagct cggccgacag
     4261 gccgaggctt tcaaatgacg tagtaatgac tttctcctga catatcggcc cgggaaggcg
     4321 cgagccttcc aaaccggttc aggctgatgc gattcgaggc tgggtgattc gacgatccag
     4381 gatggaccgt gaaacgactg tgacgctaac cggatgacgc gaaacagaaa ccttgggccg
     4441 cactatacac gagtccggcg cccgcgcaaa gcacgatcgt cgctttaaaa atagacagga
     4501 tttacaagat ttcacaggat taacaagatt agagcgaagc attcaaaacg actcagtgag
     4561 ctgttctgtc ctgtaaatct tgttgaatcc tgttaatcct gtttattttt tccccctgca
     4621 cttgcctgtg cgcgccgcgc gcgccgcagc atgaaacgcg cggggtcgat cgcgtcggcg
     4681 agatcgcttt cgacgggcag gggttcgccc tcgattgcgc tggcgatcaa ctccccgccg
     4741 agcgccgccc acgtgagccc ccgcgaggca taggcgaagg cgccgtagag gccgggtagc
     4801 cgcggcagaa ttcctgcccg cgcgcccggt tgcgcgccgg cttccggcat ggagccgatc
     4861 agcggcaggc ggtccggtgc cacgcagcgg aatcccacgg aaccggtgag cctcgatgga
     4921 tccagtcggg cggccgttcc cggcagcagt cgctcgagcc gctcgaggtt gccggcgtgg
     4981 tcctcttcgc gcggctcggc gctctcatcc tcgaaatcgt aggttgcccc cgctattgcc
     5041 gacccgtccg cgagcggtat caggtggcct gcgccggcca gcaccgcgcc gggcccggcc
     5101 aggcttcccg cgggcaggca ggtgagctgt ccgcgcacgc gcttcaacgc cacgccggtc
     5161 gcggcgagcc gccctgcgtc gttcgagttg gccagcacga cgaccggcgc cgatgcgatc
     5221 gccgtcccgt ccgccgacag gatgcgccag ccgttgccgg cgcgatcgag cgcgtgcact
     5281 gcaacgccca ggcgcaattt cagattgccg cgcgccgccg ccgcggcgat ctgggcggag
     5341 gcgagttccg ccggacgcat ccagccgctc ccggggaacc acaggccgcc gccggtgacc
     5401 gccagcgccg cgagccggcc ggcttcccca cgcggcgcga aatcgacgta gtccggcagc
     5461 ggacccagcg cgcgaacggc ggccgcgaag cgcgcttcct cccttgcgtc cctggcgatt
     5521 tgcagcacgc cgcagcgcgc ccaggcgaag cgatgaccat tgcgttcgag cgcgcgccag
     5581 ttggcggtcg cgtacagaaa tcccgcgcgg gtgagccgcg acagcaggct gtcgtcgcgg
     5641 gagacatgcg ggtgaaagac tccggcggcg atccccgacg cttccgccgc cggcgcggca
     5701 tggcgttcga tcagttcgac gcgccacccg cgcgcggcca ggcgctcgca ggccgccgcg
     5761 ccggcaagc
//
LOCUS       DBNGSG010000373         1982 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2838112, whole genome shotgun sequence.
ACCESSION   DBNGSG010000373 DBNGSG010000000
VERSION     DBNGSG010000373.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2838112"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..832)
                     /locus_tag="AC158Q_09680"
     CDS             complement(140..832)
                     /locus_tag="AC158Q_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HIR3340087.1"
                     /translation="MKIQFDSATPEAEAIRQRYLKVDAATVSDVLDSLGYQHQGLAAA
                     FAPYPETAGKLAGWAYTICGEMRPYAGGGDADKMKAVDGLKAGSVSVWSGAGDGVCFF
                     GELIALGMKTRGAAGALVDGGIRDIEWIAKQKFPVYARYRTPVQSIGRWKVTACQVPA
                     YLPGATKQHVIVNPGDFVLGDTDGVIVVPADAVEKALNEAERLTATEVKIRAELDAGA
                     SLEQVLAKYGHV"
     gene            complement(942..1757)
                     /locus_tag="AC158Q_09685"
     CDS             complement(942..1757)
                     /locus_tag="AC158Q_09685"
                     /EC_number="1.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF009829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HIR3340088.1"
                     /translation="MRVAIIGGGSIARLFIGHIRRGDLGRARVVAIAGRGGQSRGKAL
                     AKAFSIPYVTGHKALLAKKPDVVVEAASHEAVREYGAPLLGKGVSLIVLSCGALCDDA
                     LRAKLERAAARSGALLSVPSGGIGGLDALKAACVAGVDEVTIAVTKPPAAWKGIAYVE
                     KLGIDLYGLREPRVLFEGTAREGVPLFPANVNIAAVLSMAGIGFDRTRLKVVADPALR
                     HNTHFIDVKGKTGNVSIKLENVPSPDNPKTAWLACYSALAALKAVKSSVRYGT"
BASE COUNT          328 a          685 c          611 g          358 t
ORIGIN      
        1 gtcgatattg ggggttttca cgatgggatg cccggcgcag cccatcacct tggggttgtg
       61 ctcatcggac atgatgatga gcagattcgc tggtttcacg ggatgccgtt tactggctaa
      121 aggacactca acactgcaat cagacgtgac cgtacttggc gagcacctgc tcgagagagg
      181 cgccggcatc gagctcggcg cggatcttca cttccgtggc ggtcagtcgc tcggcctcgt
      241 tcagcgcttt ttccaccgcg tcagccggaa ccacgatgac gccgtccgta tcgccgagga
      301 cgaaatcgcc cggattgacg atgacatgct gcttggtcgc gcccggaagg taggcgggga
      361 cctggcacgc cgtgactttc cagcggccga tggactggac cggcgtgcga tagcgggcgt
      421 aaacagggaa tttctgcttc gcgatccact caatgtcgcg gatgccgccg tccaccagcg
      481 cgccggccgc gccgcgggtc ttcatgccga gcgcaatcag ctctccgaaa aagcacacgc
      541 cgtcgcccgc cccgctccac acgctgacgc tgccggcctt cagcccgtcc acggccttca
      601 tcttgtccgc gtcgccgccc ccggcgtacg gacgcatctc cccgcaaatc gtgtaagccc
      661 atcccgccag cttgccggcg gtctccgggt acggcgcgaa tgcggccgcc agcccctggt
      721 gctggtagcc gagcgagtcg agcacgtccg ataccgtcgc cgcgtccacc ttcaggtagc
      781 gctgccggat ggcttcggct tctggagtgg cggaatcgaa ctgaatcttc atcttggagt
      841 atgcgtgaac cgtgatccgg ttttttcatt gcgtttgttg ccgactactt gcattttcat
      901 tccatctttc ctggcggtct tggcgtcctg gcggctaatt tctacgttcc gtaacgaacc
      961 gaagacttca cggccttcaa cgccgccagt gcactatagc aggcgagcca cgcggtcttg
     1021 ggattgtccg gcgaaggcac gttctcgagc ttgatgctga cattgccggt cttgcccttc
     1081 acatcgataa agtgcgtgtt gtgcctcaat gccgggtcgg ccacgacctt gagccgggtc
     1141 ctgtcgaagc cgatgccggc catggacagc accgccgcga tgttcacgtt ggcgggaaac
     1201 aacggcacgc cctcgcgcgc cgtcccctcg aacagcacgc gcggttcgcg caacccgtag
     1261 aggtcgattc ccagcttctc gacgtaggca atacccttcc acgccgccgg cggcttggtc
     1321 accgcgatgg tgacctcgtc cacgcccgcc acgcaggccg ccttcagcgc gtcgagcccg
     1381 ccgatgcctc cggagggaac cgacagcagc gcgccgctcc gcgccgccgc gcgctcgagc
     1441 ttcgcgcgca gcgcgtcgtc gcacaacgcg ccgcaggaaa gcacgataag ggagaccccc
     1501 ttgcccagca gcggcgcgcc gtactcgcgc accgcttcgt gcgaggccgc ttcgacgacg
     1561 acatcgggtt ttttggcgag cagcgccttg tgtccggtga cataggggat gctgaacgcc
     1621 ttcgccagcg ccttcccgcg cgattgcccg ccgcgcccgg caatcgccac cacccgcgcg
     1681 cggcccaggt cgccgcgccg gatgtgcccg atgaacagcc gcgcgatgct gccgccgccg
     1741 atgattgcca cccgcatgac gactccggac gcagaacaga aaagttattt tacaggttgg
     1801 ggccgatacg ggcatttcag ccgtccgccg caaacagcag cgtcaggtcg cctcccagcc
     1861 ggtcgtagct gagcggcctc agctcggcgg ccagtcgagc agccggcgag gacgggaagc
     1921 gcgccgcata gggctccagc caggccatga tgccgccgcg cgggacacgc tgagcagcgc
     1981 cg
//
LOCUS       DBNGSG010000374         3176 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3006825, whole genome shotgun sequence.
ACCESSION   DBNGSG010000374 DBNGSG010000000
VERSION     DBNGSG010000374.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3006825"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="AC158Q_09690"
     CDS             <1..568
                     /locus_tag="AC158Q_09690"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIR3340089.1"
                     /translation="EAGSRSFVSWHQDLNYWGLDGDDEVTAWFALTPATVENGCMRFV
                     PGSHRKKDVPHVDRFAPDNLLTRGQEIAVEVDEASAVNVLLRAGQASFHHGHMFHASG
                     PNRTDSRRLGVAIRFVAPSMKQVSGERLLVSHVSGNDEYGHFEHMPAPAGRLRPEDWD
                     RARRNAGMKREILYKGVKPELVRENRRS"
     gene            complement(667..1200)
                     /locus_tag="AC158Q_09695"
     CDS             complement(667..1200)
                     /locus_tag="AC158Q_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIR3340090.1"
                     /translation="MLIAFNKPFNVLCQFTDGAGRRTLAGFVTLKGVYPAGRLDYDSE
                     GLLLLTDDGLLQKRIADPRHKMKKTYLAQVERIPDEKALALLRRGVKLKDGMTAPAGA
                     RLVEEPGWLWPRVPPIRHRRDIPTAWLELAITEGRNRQVRRMTAAVGHPTLRLIRVSI
                     GAWSLDGLRPGEWGVRR"
     gene            complement(1394..2914)
                     /locus_tag="AC158Q_09700"
     CDS             complement(1394..2914)
                     /locus_tag="AC158Q_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HIR3340091.1"
                     /translation="MPEPLLIAKSGIDLCLLPQLANRHGLIAGATGTGKTVTLQRIAE
                     GFSRIGVPVFMADVKGDLAGLSQPGEAKGKIKERIESLKLDVAFEACPVVFWDAFGSQ
                     GHPVRTTISEMGPLLLARMLGLNETQAGVLTLAFKVADDNGLLLLDLKDLRALLQHVG
                     DNAKQFTTQYGNISSASIGAIQRGLLAIDQQGGDKFFGEPALNIADLMQTDGRGRGVI
                     NILAADKLMNSPKLYSTLLLWLLSELFEELPEVGDPEKPKLVFFFDEAHLLFNDAPAA
                     LLEKIEQVVRLIRSKGVGVYFVTQNPLDIPDTVLGQLGNRVQHALRAFTPRDQKAVKS
                     AATTLRANPKLDVEAAITELGVGEALVSLLDEKGRPTVVERAFVAPPGTRIGPVTAQE
                     REKLIKSSAVAGIYDKTEDRESAYEKLAQHVNARTEAAGSAAGSTKPGAAGGGLLGSL
                     GDFLGGSTGPRGGRREGAIEAAAKSAARSIGSGLGREIVRGVLGGIFGGSTTRRRR"
     gene            3054..>3176
                     /locus_tag="AC158Q_09705"
     CDS             3054..>3176
                     /locus_tag="AC158Q_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR3340092.1"
                     /translation="MAGHSKWANTQHRKNRQDAKRGKAFTRLIKEITVAARLGGG"
BASE COUNT          538 a         1040 c         1068 g          530 t
ORIGIN      
        1 ggaggcgggc tccagaagct tcgtgagctg gcaccaggat ctcaattact ggggcctcga
       61 cggggacgat gaggtcacgg cctggttcgc cctcacgccg gcgacggtcg agaacggctg
      121 catgcgtttc gtccccggca gccaccggaa aaaggacgtg ccgcacgtgg acaggttcgc
      181 gccggacaac ttgctgacgc gcggacagga gatcgcggtc gaggtggacg aggcgagcgc
      241 ggtgaacgtc ctgctgcgcg ccggccaggc ttcgttccat cacgggcaca tgttccacgc
      301 atcgggcccc aaccggacgg attcgcgccg cctgggtgtc gccatccgtt tcgtggcgcc
      361 gtcgatgaaa caggtctccg gcgagcggct gctggtttcg cacgtgagcg gcaacgacga
      421 atatgggcac ttcgagcaca tgcccgcgcc cgcgggccgg ctgcggccgg aagactggga
      481 ccgcgcccgc cgcaacgccg gcatgaagcg cgaaatcctc tacaagggcg tcaagccgga
      541 gctggtcagg gaaaaccgca ggagttgatg aagaaccgtg gcgggcggcg acgaagtgag
      601 gcagtgacgg ggtgacgaag ggcggttcgt ctcatccttc gcaagcacgg catcactcct
      661 ctcagatcag cgccggactc cccactcacc cggccgcagg ccgtcgagcg accacgcgcc
      721 gatgctcacg cgtatgagcc gcagagtcgg atggcccacg gcggcagtca tgcggcgaac
      781 ctggcggttg cggccttccg tgatcgcgag ctccagccac gccgtgggaa tgtcgcggcg
      841 atggcgtatc ggcggcacgc gcggccacaa ccagccgggt tcctccacca gccgcgcgcc
      901 cgccggcgca gtcatgccat ccttcaattt cacgccgcgg cgcaggagcg ccagcgcttt
      961 ctcgtccggg atgcgctcca cctgggcgag ataggtcttc ttcatcttgt gacgcgggtc
     1021 ggcgatgcgc ttctggagca agccgtcgtc ggtcaggagc agcaagccct cgctgtcata
     1081 atcgagccgg cccgccggat agacaccctt cagcgtcacg aacccggcca gcgtgcgccg
     1141 gcccgcaccg tcggtgaact ggcagagcac gttgaacggt ttattgaagg cgatcagcat
     1201 gctcaaccgt tctaacaagg tattgaaaaa cgctcgtcgc tcccgcggag gcgggagccc
     1261 agaaaactgg cttcaaaccg aatttacacg ttactggatt cccgcctgcg cgggaatgac
     1321 gaacccgggg tgtcgttcgg tttcttgaac gaactgatac cagcttatgg gatcttttgc
     1381 gtcgcctcgc ggcttagcgg cgccgccggg tcgtgctgcc gccgaagatg ccgcccagta
     1441 ctccgcggac gatctcgcgc cccaatcccg aaccgatgga gcgtgcggcg ctcttcgccg
     1501 ccgcctcgat ggcgccttcg cgccgcccgc cgcgcggacc ggtcgagccg cccaggaagt
     1561 cgccgagcga gccgagcaag ccgccgccag cggcgccagg cttcgtcgag ccggccgcag
     1621 atcccgccgc ctcggttcgc gcattgacgt gctgcgcgag cttttcataa gccgactcgc
     1681 ggtcttccgt cttgtcgtag atacccgcca ctgccgagga cttgatcagc ttctcgcgct
     1741 cctgcgccgt gacgggcccg atgcgcgtgc ccggcggcgc cacgaaggcg cgttccacca
     1801 ccgtggggcg gcccttctcg tccagcaacg agaccagcgc ctcgccgacg ccgagctcgg
     1861 tgatcgccgc ttcgacgtcg agcttggggt tcgcccgcaa cgtggtggcg gcggacttga
     1921 cggctttctg atcgcgcggc gtgaaggcgc gcagcgcgtg ctgcacgcgg ttgccgagct
     1981 ggcccagcac ggtatccggt atgtcgagcg ggttctgcgt gacgaagtag acgccgacgc
     2041 ccttggagcg gatcaggcgc accacctgct cgatcttttc cagcagggcg gccggcgcgt
     2101 cgttgaacag caggtgcgcc tcgtcgaaga agaacacgag cttcggcttc tcggggtcgc
     2161 ccacttccgg cagttcctcg aacagctccg agagcagcca caggagcagg gtcgagtaga
     2221 gcttgggcga gttcatcagc ttgtcggcgg cgaggatgtt gatgacgccg cggccgcgcc
     2281 cgtcggtctg catcaggtcg gcgatgttga gcgccggctc gccgaaaaac ttgtcgccgc
     2341 cctgctggtc gatcgcgagc agtccgcgct ggatcgcgcc gatcgacgcg gaggaaatgt
     2401 tgccgtactg ggtcgtgaac tgcttggcgt tgtcgccgac gtgctgcagc agcgcgcgca
     2461 ggtccttgag atcgagcaac agcagtccgt tgtcgtcggc gaccttgaag gcgagggtca
     2521 ggacgccggc ctgcgtttcg ttgagcccca gcatgcgcgc gagcagcagc ggccccattt
     2581 cggagatcgt ggtgcgcacc gggtggccct ggctcccgaa cgcgtcccag aacaccaccg
     2641 ggcaggcttc gaaggcgacg tcgagcttca ggctctcgat ccgctccttg atcttgcctt
     2701 ttgcctcgcc cggctgcgac aggcccgcga ggtcgccttt cacgtcggcc atgaacaccg
     2761 gcacgccgat gcgggagaac ccttcggcga tccgctgcag cgtcaccgtc ttgccggtgc
     2821 cggtcgcgcc ggcgatcagg ccgtggcggt tggcgagctg gggcagcaga cagagatcga
     2881 tcccggattt ggcgatgagc aagggctcgg gcatggcgga ctcgcgtgat aaaattttgg
     2941 cgaaagaacg aggggatgct cccctcgtgt tccctacatc aggggccgtt acctttcttt
     3001 caaaacggcc tcgcggtgat tttaacgaca taagcaggcg gcaggggcaa caaatggcag
     3061 gccatagcaa gtgggctaac acgcagcatc gcaagaaccg ccaggatgcg aagcgcggca
     3121 aggccttcac ccggctgatc aaggaaatca ccgtggccgc gcggctgggc ggcggc
//
LOCUS       DBNGSG010000375         5335 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1479631, whole genome shotgun sequence.
ACCESSION   DBNGSG010000375 DBNGSG010000000
VERSION     DBNGSG010000375.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5335)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5335)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5335
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1479631"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="AC158Q_09710"
     CDS             <1..747
                     /locus_tag="AC158Q_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3340093.1"
                     /translation="AKDAHKIIITLSNLFTTPLATGVPFNWKDLTPVAMLALDNFVLF
                     VNAETPYKSPKDYIDAAKKAGPGKFKMAGTGAKQEDQIITVAIEKAGGVKFTYVPFKG
                     GGAVATQLVGKHVDSTVNNPIEAVAHWRSGKLRALCVFDGSRMPYKAKVTETMSWYDI
                     PTCKEAGIPMEYTMLRGIFMPAGVSKDVVDYYVAFFQKVRGTPEWKDFMEKGAFNQTF
                     MSGKEYADWVAKAEVLHRNLMSDAGFLAKK"
     gene            851..1357
                     /locus_tag="AC158Q_09715"
     CDS             851..1357
                     /locus_tag="AC158Q_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HIR3340094.1"
                     /translation="MEQNGRSAQRAVRVVTMDLVVAALIFALGALVVFESVRLGASWG
                     SDGPGAGYFPFYIGMLICICSFAVFVQSLLKRKVDRAVFVTEEQFKLVLVVLVPTTAY
                     AVGVWLIGMYVSSALFIVLFMRIAGHYTWLRSTAVGAGVSVAAFVMFEIWFKIPLPKG
                     PVEALIGF"
     gene            1385..2899
                     /locus_tag="AC158Q_09720"
     CDS             1385..2899
                     /locus_tag="AC158Q_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR3340095.1"
                     /translation="MEELNALFHGFSVALTPFNLMLMLIGITLGVIIGVLPGLGGANG
                     IAILLPLTFSMSPTSAIIMLSCIYWGALFGGAITSILFNIPGEPWSVATTFDGYPMAQ
                     QGRAGEALTGAFTSSFVGALVAVLMFTFIAPLVANFALQFGPPEFFAVYLLTFCSFVG
                     TGKGNPFKIVASMMLGFAMAAVGIDIVTGQLRLTFGFTELLSGFHFLVAVIGLFGIGE
                     ILLSMEEGLSFTGHTAKINPKIVLETWKKLPRYWATSIRGCIIGCWMGIIPGGATPAS
                     FMSYGVARKVSKDGDKFGTGQLEGVVAPETAAHAAGTSALLPMITLGIPGSPTAAVLL
                     GGLLIWGLQPGPLLFVENKDFVWGLIASMYLGNVVGLIVVLTMVPLFAAILRVPFSII
                     APVIVVICAIGAFTVKNNMLDVGLMVVFGVVGYVFKKLDYPLAPLVLALVLGDAAESA
                     FRQSMLISQGDLTVFFSNWLVGGMTGLALFALFWPLISRGISRLRTSGRGGTAV"
     gene            complement(2915..3523)
                     /locus_tag="AC158Q_09725"
     CDS             complement(2915..3523)
                     /locus_tag="AC158Q_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3340096.1"
                     /translation="MTLLVRHPEAKIIRVQLRQNFILKHVEAAQWRELEPLLEIVDYA
                     KGDLLEHQGDWSMEQYFIIDGILKRVVSNAQGKEMILRFAAESDIDTSYAAWCLKTPI
                     PYSIRAVTKVRVAKTSMPHWVRFLGRHATIKSAFEFEIMKLMSEVMAHTITLHLLDAP
                     GRIRRFMRKHADLVDRLPKKELALYLNLSPETLSRLKGRGKI"
     gene            3676..>5335
                     /gene="oxc"
                     /locus_tag="AC158Q_09730"
     CDS             3676..>5335
                     /gene="oxc"
                     /locus_tag="AC158Q_09730"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HIR3340097.1"
                     /translation="MGNVENPVASTAASVQQPAQEMMDGFSLVIDALKLNGIDTIYGL
                     PGIPITDLTRRAQAAGLRVLSFRHEQNAGYAASIAGFLTQKPGICLTVSAPGFLNGLN
                     ALAHATVNCFPMILISGSSEREIVDLQQGDYEEMDQLAVAKPVAKAAFRVLHAEDIGV
                     GVARAIRAAVSGRPGGVYLDLPAKLFAQSIDAEAGRKVLIKVVDPAPKQIPAPDAVKR
                     ALDLLKSAKKPLIILGKGAAYAQADADIRSLVEKSGIPYLPISMAKGLLPDTHELCAS
                     AARSYVLPEADVVMLIGARLNWLLSHGKGKTWGGKSSKSWGGQKFIQVDISPQEADSN
                     VRIDAPVVGDIGSCVSALLAGMGTGWTKPSTEWMGSIFERKSKNIEKMAETLAKNPSP
                     MNFHSALNVVRDIVKANPDAILVNEGANALDFTRSIVDMYKPRKRIDVGTWGIMGVGM
                     GYCVAAAVVSGQPVIAIEGDSAFGFSGMEVETICRYNLSVCVVVFNNNGVYRGTDVNP
                     TGGPDVAPTVFVKGARYDKLIEAFGGVGVHATTPAELRKAMEEAV"
BASE COUNT          948 a         1589 c         1740 g         1058 t
ORIGIN      
        1 gccaaagacg cgcacaagat catcatcacg ctgtccaacc tgttcacgac gccgctcgcc
       61 acgggcgtgc cattcaactg gaaggacctg acaccggtgg cgatgctggc gctggataac
      121 ttcgtgctct tcgtcaacgc tgaaacgccg tacaagagcc cgaaggatta catcgatgcg
      181 gccaagaaag cgggcccggg caagttcaag atggccggta ccggcgccaa gcaggaagac
      241 cagattatca cggtcgccat cgagaaagcg ggcggcgtca aattcaccta tgtgccgttc
      301 aagggcggcg gcgcggtagc cacccagttg gtcggcaagc acgttgactc cacggtcaac
      361 aacccgatcg aggccgtggc gcactggcgc tcgggcaaat tgcgcgcgct gtgcgtattc
      421 gacgggagcc gcatgccgta caaggcaaag gtcacggaga cgatgtcctg gtacgatatt
      481 ccgacgtgca aggaagccgg catcccgatg gagtacacga tgctgcgcgg cattttcatg
      541 cccgccggcg tatcgaagga tgtcgtcgac tactacgttg ccttcttcca aaaggtccgc
      601 gggacgccgg aatggaagga tttcatggaa aagggcgctt tcaaccagac tttcatgtcg
      661 ggcaaggagt acgccgactg ggtcgcgaaa gcggaagtcc tccaccgcaa cctgatgtcg
      721 gacgcgggct tcctggccaa gaagtagcag aaccctggag gacgggcagc acgcccgtcc
      781 tccagtttct tcaaagcggg ggtggtcgtc cacgccccag tgcttcccca tagacccggc
      841 agcggcccac atggaacaaa acggcagatc ggcgcagcgc gcggtgcgcg tcgtgaccat
      901 ggacctcgtc gtcgcggcgc tgatcttcgc gctgggtgcg ctggtggtct tcgaaagcgt
      961 acggctgggc gcgagctggg gcagcgacgg cccgggggca ggctatttcc cgttctatat
     1021 cgggatgctg atctgcattt gcagtttcgc ggtgttcgtc caatccctgc tcaaacgcaa
     1081 ggtcgaccgg gcggttttcg tcaccgagga acagttcaag ctggtgctgg tggtcctcgt
     1141 tcccaccacc gcctatgccg ttggtgtgtg gctgatcggc atgtatgtct cgtcggccct
     1201 gttcatcgtg ttgttcatga gaatcgcggg ccattacacg tggctgcgca gcacggccgt
     1261 gggggcgggg gtcagcgtgg ctgcgttcgt gatgttcgag atctggttca agatcccgtt
     1321 gccgaaaggc ccggtcgagg cgctgatcgg cttctaggcg gcaacacagg ggaggtagtt
     1381 caagttggaa gaactcaacg cgttgttcca cggcttttca gtcgcgctga cgccgttcaa
     1441 cctgatgctg atgctgatcg gcatcacact cggcgtaatc atcggcgtgc tgccggggct
     1501 gggcggggcg aacggcatcg ccattctgct gccgctcacg ttctccatgt cgccgacctc
     1561 ggcgatcatc atgctctcct gcatttactg gggcgcgctg ttcggcggcg cgatcaccag
     1621 catattgttc aatattcccg gcgagccgtg gtcggtcgcg acgaccttcg acggataccc
     1681 catggcgcaa cagggccggg cgggagaggc gctgacgggg gcgttcacct cgtcgttcgt
     1741 gggggcgctg gtggcggtcc tgatgttcac cttcatcgcg ccgctggtcg caaattttgc
     1801 gctgcaattc gggccgccgg agtttttcgc ggtctatctc ctgaccttct gcagcttcgt
     1861 cggcaccggc aagggcaatc ccttcaagat cgtcgcctcg atgatgctgg gcttcgccat
     1921 ggctgcggtc ggcatcgaca tcgtaaccgg ccagctgcga ctcacgtttg gattcacgga
     1981 actgctgtca gggttccatt tcctggtggc ggtgatcggg ctgttcggca tcggcgagat
     2041 actgctgtcg atggaggaag ggttgtcgtt cacgggacac acggccaaga tcaatcccaa
     2101 gatcgtgctg gaaacctgga aaaagctgcc gcgctactgg gcgacttcga ttcgcggctg
     2161 catcatcggc tgctggatgg ggatcattcc ggggggggcc acgccggctt ccttcatgag
     2221 ctacggcgtg gcgcgcaagg tctcgaagga tggcgacaaa ttcggcaccg gacaactcga
     2281 aggcgtggtt gcgccggaga ccgcggcgca tgccgccggg acctcggcgc tgctgccgat
     2341 gatcacgctc ggcatccccg gatcgcccac ggccgcggtg ctgctcgggg ggctcttgat
     2401 ctgggggctg cagccgggac cgctgctgtt cgtcgagaac aaggacttcg tgtggggcct
     2461 gatcgccagc atgtacctgg gcaacgtcgt gggcctgatc gtcgtgctca ccatggtgcc
     2521 gctgttcgcc gccatactgc gcgtcccgtt ctcgatcatc gcgccggtga tcgtggtgat
     2581 ttgtgccatc ggggccttca cggtcaagaa caacatgctc gacgtgggcc tgatggtggt
     2641 gttcggcgta gtgggttatg tcttcaagaa gctggattac ccgcttgccc cattggtact
     2701 ggcgctggta ctgggcgatg ctgccgagag cgcgttccgg cagtcgatgc tgatctcgca
     2761 gggtgacctg acggtgtttt tctcgaactg gctggttggc ggtatgaccg gcctggcttt
     2821 gttcgcgctg ttctggccgc tgatctccag gggcatctca cggctgagga cgtccgggcg
     2881 cggtggcacc gcagtctagc cggtgtccgt gcggtcagat cttgccgcgg cccttcaacc
     2941 gcgacagggt ttcgggcgag aggttgagat agagcgcgag ctctttcttg ggaaggcgat
     3001 cgacgaggtc ggcatgcttg cgcatgaaac gccgtatgcg cccgggcgca tctagcagat
     3061 gcagcgtgat ggtatgggcc atgacttcgc tcatcagctt catgatttcg aattcgaaag
     3121 cgctcttgat cgtggcgtgg cggccgagga accgcaccca gtgtggcatg gatgtcttgg
     3181 cgacgcgcac cttggtcacg gcccggatcg aatagggaat gggcgtcttc aggcaccagg
     3241 cggcgtagct ggtgtcgata tcgctttcgg cggcaaagcg caggatcatt tccttgccct
     3301 gggcattcga caccactcgc ttgaggatgc catcgatgat gaaatactgc tccatggacc
     3361 aatcgccctg atgctcgagc agatcaccct tggcataatc cacgatctcg agcaacggct
     3421 cgagctcgcg ccattgcgcg gcctcgacgt gtttgaggat gaagttctgc cgcaactgga
     3481 cgcggatgat cttcgcttcc gggtgcctga cgaggagggt catcggagtc gctgcggtgt
     3541 aacggggcgg ctggattgcc cagcgagtat aaccgcaggt tcttgaccgg ggtcaaggct
     3601 gtcgtttcgg cgggagccta tattagacaa cctttcaata cgttagcaac gcctccgtcc
     3661 ttcaaggaga gacttatggg aaacgtggaa aacccggtcg cctcgaccgc ggccagcgtg
     3721 cagcaacccg cacaggaaat gatggacggc tttagcctgg tcatcgacgc cctcaagctc
     3781 aatggcatcg acacgattta cggcctgccc gggatcccga tcaccgatct cacccgccgg
     3841 gcgcaggccg cgggcctgag agtgctctcg ttccgccacg agcagaatgc aggatatgcc
     3901 gcgtcgatcg ccggtttcct gacgcagaag ccgggcatct gcctcacggt gtccgcgccg
     3961 ggctttctga acggcctgaa cgcgcttgcc catgccaccg tcaactgctt tccgatgata
     4021 ctcatcagcg gctcgagcga gcgcgagatc gtcgatcttc agcagggcga ttacgaggag
     4081 atggaccagc tcgcggtcgc caagccggtc gccaaggctg ccttccgcgt cctgcacgcc
     4141 gaggacatcg gcgttggcgt cgcgcgcgct atccgcgccg cagtctccgg ccgccccggc
     4201 ggggtttacc tcgatctgcc ggcgaagctt ttcgcgcaaa gtatcgatgc cgaagccggc
     4261 cgcaaggtgc tgatcaaggt cgtcgatccg gcgccgaagc agattcccgc gcccgatgcg
     4321 gtgaagcgcg cgctcgacct gctgaagagc gcgaagaagc cgctcatcat cctcggcaag
     4381 ggcgcggcgt acgcgcaggc cgacgccgac atccggtcgc tggttgaaaa gtccggaatt
     4441 ccctacctgc cgatttcgat ggccaagggg ctgctgcccg acacgcacga gctgtgtgcg
     4501 tcggccgcgc gctcgtacgt gctgccggag gcggacgtgg tgatgctgat cggcgcgcgc
     4561 ctgaactggc tgctgtccca cggcaagggc aagacctggg gtggcaagtc ttccaagtcc
     4621 tggggtggcc agaaattcat ccaggtcgat atttcaccgc aggaggccga cagcaacgtg
     4681 cgcattgacg cgccggtggt cggcgacatt ggttcctgtg tctcggcgct gctagccggc
     4741 atggggacag gctggaccaa gccgtcgacg gagtggatgg gctccatttt cgagcgcaag
     4801 agcaagaata tcgagaagat ggccgagacg ctggcgaaga atccctcgcc gatgaatttc
     4861 cacagcgcgc tcaacgtggt gcgcgacatc gtcaaggcga atccggacgc gatcctcgtc
     4921 aacgaaggcg ccaacgcgct cgacttcacc cgcagcatcg tcgacatgta caagccgcgc
     4981 aagcgcatcg acgtcggcac ctggggcatc atgggcgtcg gcatgggcta ctgcgtcgcc
     5041 gctgccgtgg tcagcggtca gccggtgatc gcgatcgagg gggacagtgc cttcggcttc
     5101 tccggcatgg aagtcgagac catctgtcgc tacaacctgt cggtttgcgt ggtggtcttc
     5161 aataacaacg gcgtctaccg cggcaccgat gtcaatccga ccggcggtcc cgacgtggca
     5221 cccaccgtgt tcgtcaaggg tgcgcgctat gacaagctga tcgaggcttt tggtggcgtg
     5281 ggcgtgcacg ccacgacccc ggccgaattg cgcaaggcaa tggaagaagc cgtcc
//
LOCUS       DBNGSG010000376         5941 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4366879, whole genome shotgun sequence.
ACCESSION   DBNGSG010000376 DBNGSG010000000
VERSION     DBNGSG010000376.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5941)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5941
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4366879"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..291)
                     /locus_tag="AC158Q_09735"
     CDS             complement(67..291)
                     /locus_tag="AC158Q_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HIR3340098.1"
                     /translation="MAKGKSKGSVYRVTELVGTSRNSWEDAARNAVEVAGRSLRDLRV
                     AEVTKTDLVIEDGKVTAYRTRVSLSFKYQR"
     gene            complement(486..2660)
                     /locus_tag="AC158Q_09740"
     CDS             complement(486..2660)
                     /locus_tag="AC158Q_09740"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3340099.1"
                     /translation="MLNGFLKLIIGALYRVRVEGDAAQLRNGRVLIVANHDSMLDGLL
                     LGLFLPVPVTVAIAGDGLRHHPLLRLLMRGVPHVILDPSRPLALKRLIRQVERGQPVV
                     VFPQGPATSTGGLTKIYDSAAVIAARCNARIVPVRITGTLYTRFAAVGGLFPRQRFPA
                     VTIRILEARQLPVPSPQSGKKKRRLLADALLRIMQHMMFASLPARTLFESLLDVAALH
                     GRSTRILEDTQREHTYGDLIKASLALGRLGAKVAGEGEIVGVLMPNLAATVALLFGLS
                     SVRRTPALLNYTAGPDALRSACIAAGIKTLITSRRFVDIAKLGSSIAALSTVRIVYLE
                     DLRSRFGVLDKLWLLGWALWRPSSVIRERDPDETAIVLFTSGSEARPKGVALSHRALL
                     ANIAQMSAVIDFGPNDKFLNSLPMYHAYSLTACTLQPLLTGTRMYLSTTPLHYHVSPE
                     LAYTRACTYVFGTSTFLAHYARQAHPYDFHKVRVVVSGAEKLNPEVAGLWLNKFGLRI
                     MEGYGATECAPVISLNTPLCYRPGTVGRLLPGIEHRVLPVPGIARGGVLHVRGPNVMR
                     GYYLYDQPETLQPPHSEAGEGWYNTGDVVELDEEGFVTIHGRVKRFAKIAGEMVSLEV
                     VERIAQHASPGHQHAAALELISQSGESTVLFTTDPQLDRIALQKAARELGAQDLAVAR
                     RLVHVASLPLLGSGKTDYVTLKSLTSARRSAEQELLSGDATA"
     gene            2952..3878
                     /locus_tag="AC158Q_09745"
     CDS             2952..3878
                     /locus_tag="AC158Q_09745"
                     /inference="COORDINATES: protein motif:HMM:NF006452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HIR3340100.1"
                     /translation="MEMHVPSAVLEVRHVPAIAPLASGIALSFSPQLAVHYDKDARAI
                     WSRWAPEPRPCFNPALLADIRAYYEFLAASKARIDCFGEEHDIEYVVLASATHGVFNL
                     GGDLDLFKQLIDARDRQGLLRYGRACIDVLYRNYVGHDLPVTTVSLVQGECLGGGFEA
                     ALSSDVIIAERSARFGFPEILFNLFPGMGAYSFLERRIGQRGAENLIASGKIYSAEEM
                     AALGVIDRVAEDGNGEVEVGALIRQRTRSRNGHSALAAARRRVHRIEFPELLDIVQIW
                     VDSALRLNPRDLKLMQRLVSRQNGLNDSHQIH"
     gene            4063..>5941
                     /locus_tag="AC158Q_09750"
     CDS             4063..>5941
                     /locus_tag="AC158Q_09750"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR3340101.1"
                     /translation="MLSIPIQKLRRWDEQGVLVAGRTHGGHRRYSREVVERLAGAGLS
                     TGADKTNKELATIRKSLAEKRRIIQLLLESENRYRDLVETSHDLIWTTDAQGRFTYLN
                     NAANVIFGLAPKDLIGRCFFDFEAGTSHVSNRRFLATLKRSGEVKHYVTRLMTAKGQD
                     RWIGINARVVLDDEGAMLGIRGTARDITEEHLATRRIEHLALHDSLTDLPNRHSLQRR
                     IEAALHADGVGALLFLDIDHFKYVNDNFGHRTGDQLIIGVGSVLRDLARGIDGELFRL
                     GGDEFAIHLPAALRREAIEVAEKALDAVRRYRFQAADNKVISNLAASAGIALYPFHGA
                     DVLALLSNVDIAMYQAKDLGRNRHMLFDQASGNLRSTHRRIHWAKKLRDAIDEDRLVL
                     FHQPVVRLKDRKPVHHEILVRIRDESGGFILPGHFIELAESLGLIQEIDMRVVEKLLA
                     FLRSSGQANRKLRYFVNLSRVSISDQHWIQRFTAMLRESGVNPNQLVFEITETAAMSE
                     IDVTLTFIKRLKEMGCRFALDDFGAGFSSFYYLKRFDVDYLKIDGSFIRDLATDEGSR
                     IFVRALNDVAHGLDKQVIAEWVETPEVQTLLLGMGTQFGQGYLFQRPTLLEHAAKPAL
                     DN"
BASE COUNT         1098 a         1980 c         1845 g         1018 t
ORIGIN      
        1 ggagctttag tagcggcggg tcaccgcccg caatgaaaag gccaacgcgc gctggccttt
       61 tttgcgtcat ctctggtact tgaaagatag gctcacgcgc gtgcggtagg cggtaacttt
      121 cccgtcctcg atcaccaggt cggtcttcgt gacttccgcc acccgcaggt cgcgcagcga
      181 gcgccccgcc acttccacgg cattgcgcgc ggcatcctcc caggaattgc ggctggtgcc
      241 gaccagttcg gtgacgcggt agacgctgcc ctttgatttg cccttggcca tggtgccctc
      301 ctgttgttgg agtcacgcgc cgcgactaag acaccacgcg cggcgtggtg acaattcatc
      361 atacacccgg acgggaaatc agcagcagaa gggagcggcg tcaattccgg gggtgcggca
      421 gggccgcggc ggcctctgcc acgttacttc caatcggaat cgaaacgcgt cgcccgcaag
      481 cgcgtctacg cggtcgcatc ccctgaaagg agctcctgct ctgcgctccg gcgcgcactc
      541 gtcagcgact tgagcgtgac gtagtcggtc ttgccgctgc cgagcagggg aagcgaggcg
      601 acgtgaacca ggcgccgcgc gaccgcgagg tcctgcgcgc cgagttcgcg cgccgctttc
      661 tgcagcgcga tgcggtcgag ctgcgggtcg gtggtgaaga gcacggtgct ctcccccgac
      721 tgcgagatca actccagcgc cgcggcgtgt tgatggccgg gcgaggcgtg ctgggcgatg
      781 cgctccacca cttcgagcga caccatctcg cccgcgatct tggcaaagcg cttgacgcgt
      841 ccgtgtatgg tgacgaaacc ctcctcgtcc agttccacca cgtcgccggt gttgtaccag
      901 ccctcgcccg cttcggaatg gggcggctgc agcgtctcgg gctggtcata taaataataa
      961 ccgcgcatga cgttgggccc gcgcacgtgc agaacgccgc cacgcgcgat gcccggcacc
     1021 ggcaatacgc ggtgctcgat gcccgggagc aggcgcccga ccgtgccggg ccggtagcat
     1081 aacggcgtat tgagggagat caccggcgca cattccgtcg ccccgtagcc ctccatgatg
     1141 cgcagaccga acttgttgag ccacaggccc gccacctcag gattcaattt ctcggcgccc
     1201 gagaccacga cgcgcacctt gtgaaaatcg tacggatgcg cctgtcgcgc gtaatgcgcg
     1261 aggaaggtgc tggtgccgaa gacgtaggtg caggcgcgcg tgtacgcgag ctcgggactc
     1321 acgtggtaat gcagcggcgt cgtggagaga tacatgcgcg tgccggtcag cagcggctgc
     1381 agcgtgcagg ccgtcaggct gtaggcgtga tacatcggca gcgagttcag aaacttgtcg
     1441 ttggggccga aatcgatcac ggcgctcatt tgcgcgatgt tggcgagcag cgcccggtgg
     1501 ctcagggcga cgcctttcgg ccgcgcctcc gagccggacg taaatagcac gatcgcggtc
     1561 tcgtccggat cgcgctcccg gatgacggag ctcggccgcc acagcgccca cccgagcagc
     1621 cagagcttgt cgagcacgcc gaaacgggaa cgcagatctt ccagatagac aatgcgcacc
     1681 gtggagagcg cggcgatcga gctgcccagc ttcgcaatat caacgaaacg ccgtgacgtg
     1741 atcagcgtct tgatgccggc agcgatgcag gcgctgcgca gtgcgtcggg gccggccgtg
     1801 tagttgagga gcgcaggcgt gcgccgcacc gaggagaggc cgaacagcag cgcgacggtc
     1861 gcggcaagat tgggcatcaa caccccgacg atttcgccct ccccggcgac cttggcaccc
     1921 agccggccca gtgcgaggct ggccttgatc aggtcgccgt acgtatgctc acgctgcgtg
     1981 tcctccagaa tgcgggtgga acgcccgtgc agcgccgcga cgtcgagcag ggattcgaac
     2041 agcgttcgcg ccggcagcga cgcaaacatc atgtgctgca tgattctgag cagggcgtcc
     2101 gccagcaggc gccgcttttt cttgcccgac tgcggcgagg gcaccggcag ctgccgggcc
     2161 tcgaggatcc ggatcgtgac cgcggggaac cgctgcctgg gaaacagccc cccgaccgcg
     2221 gcaaaacgcg tgtacagcgt gcccgtgatg cgcaccggca cgatgcgggc attgcagcgc
     2281 gcggcaatga cggcggccga atcgtagatc ttggtcaggc cgccagtgga agtcgccggc
     2341 ccctgcggaa ataccacgac cggctggccg cgctcaacct gccgtatcag ccgctttaat
     2401 gccagcggcc gcgatgggtc caggatgacg tgcggcaccc cgcgcatcag cagccgcagc
     2461 agcgggtggt ggcgcaagcc gtcgccagcg atcgccaccg tgaccggcac cggcagaaac
     2521 aggcccagca gcaggccatc cagcatcgaa tcgtgattgg cgacgatcag cacccgcccg
     2581 ttacgcagct gggcggcatc cccctcgacc cgaacgcgat acagcgcgcc gattatcagc
     2641 tttaaaaatc cgtttagcac aatgacttac tggttattat taatcttgat tatcagctgt
     2701 ggttcgcttc gacgtcaatt ctcgaggcta cgccgaaccc cctccccttg ttgcgaagta
     2761 cgccacagat caagcgctgt cactctcccc ggccccgcgc tgacgtatcg ttagcctctg
     2821 tttctaaacc acaaagttaa aatctatcaa aatgttagat ttgattttaa atgcaagctt
     2881 ggcatttaat cgaagtgtga cctagatcac acacgcattg cttaattgct tctctacagg
     2941 ggagagtcat catggaaatg cacgtcccgt ccgcggtact cgaagtccgt cacgttcccg
     3001 ccatcgcgcc tctggcaagc ggtattgcgc tcagcttctc gccccagctc gcggtgcatt
     3061 acgacaagga cgcgcgcgct atctggtcgc gctgggcgcc ggaaccgcgc ccgtgcttca
     3121 atcccgccct gctcgccgac atccgcgcct attacgaatt tctcgccgcg agcaaggccc
     3181 gcatcgattg tttcggtgaa gagcacgaca tcgagtacgt ggtgctcgcc tcggccacgc
     3241 acggcgtgtt caacctgggc ggcgacctcg atctgttcaa gcaactgatc gatgcccgcg
     3301 accgccaggg gctgctgcgc tacgggcgcg cctgcatcga tgtcctttac cgcaattacg
     3361 tcggtcacga cctgcccgtt accaccgtct cgctggtgca gggagaatgc ctcggcggcg
     3421 gattcgaggc cgcgctttca agcgacgtca tcatcgccga gcgcagcgcg cggttcggtt
     3481 ttcccgaaat cctgttcaac ctgttcccgg gaatgggcgc ctattccttt ctggagcgcc
     3541 gcatcggcca gcgcggcgcg gagaacctga ttgccagcgg caagatctac agcgcggagg
     3601 aaatggccgc gctcggcgtg atcgaccgcg tggcggaaga cggcaacggc gaggtcgagg
     3661 tgggggccct gatccgtcaa cgcacccgca gccgcaatgg gcactccgcc ctggcggcgg
     3721 cacggcggcg cgtgcatcgc atcgagttcc cggagctgct cgatatcgtg cagatctggg
     3781 ttgacagcgc gctgcgactg aatccgcgcg acctgaagct catgcaacgc ctggtttcgc
     3841 gccagaacgg gctgaacgat tcccaccaga tccattgaga gccctttccc gttcgcgcgg
     3901 cccgcgaggc tttggccagt gccccaccac tctgctagac ttatgagccg tgccaagcat
     3961 aaaaatccgc ggcccagagc cgcggccagg caggccaagc ctgacaggga ggcggttgtg
     4021 agcaagccgg aaacggaatt ctccattcag caagtgtctg aaatgctgag tattccgatc
     4081 cagaagctgc ggcgctggga cgagcagggc gtgttagtgg ccggtcgcac tcacggcgga
     4141 caccgccgct actcccggga agtggtcgag cgcctcgcgg gcgccggctt gagcacagga
     4201 gccgacaaga ccaacaagga gctggcgacg atacgcaagt cgctcgccga gaagcgccgc
     4261 atcatccagc tcctgctgga gagcgagaac cgctaccgcg atctggtcga gacttcccac
     4321 gatctcatct ggaccaccga cgcgcagggc cgcttcacct acctcaataa cgcggccaac
     4381 gtcattttcg gactcgcgcc caaggacctc atcggccgct gcttcttcga tttcgaggcc
     4441 ggcacatccc acgtttccaa ccgtcgtttc ctcgcgacat tgaagcgcag cggcgaggtc
     4501 aagcactacg tcacccgcct gatgaccgcc aagggccagg accgctggat cggcatcaac
     4561 gcgcgcgtgg tgctcgacga cgagggcgcc atgctcggca tccgcggcac cgcccgcgac
     4621 atcaccgagg agcatttggc gacgcgacgc atcgagcacc tcgcgctgca cgattcgctc
     4681 acggacctgc ccaaccggca cagcctgcag cgccgcatcg aggcagccct gcacgcggac
     4741 ggcgtcggcg cgctgctgtt cctcgacatc gaccacttca agtacgtcaa cgacaatttc
     4801 ggccaccgca ccggcgatca gctaatcatc ggcgtcggca gcgtgctgcg cgacctggcc
     4861 cgcggcatcg acggcgagct gttccgcctc ggcggcgacg agttcgcgat ccatctcccc
     4921 gcggcgctgc gcagggaggc gatcgaggtc gccgagaagg cgctcgacgc cgtgcgccgg
     4981 taccgcttcc aggcggccga caacaaggtc atatccaacc tggcggcttc ggcgggcatc
     5041 gcgctctacc cgttccacgg ggccgacgtc ctcgcgctgc tgtcgaacgt ggatatcgcg
     5101 atgtaccagg cgaaggacct cggccgcaac cgccacatgc tgttcgatca ggcctccggg
     5161 aacctgcgca gcacccatcg ccgcatccac tgggcgaaaa agctgcgcga cgcgatcgac
     5221 gaggaccgcc tcgtcctgtt ccaccagccg gtggtgcgac tgaaggaccg caaaccggtg
     5281 caccacgaaa tcctggtgcg catccgcgac gagagcggcg gtttcatcct gcccggccat
     5341 ttcatcgagc tcgccgagtc gctcggcctg atccaggaaa tcgacatgcg ggtggtggaa
     5401 aagctgctcg ccttcctgcg ctcgagcgga caagccaacc gcaagctgcg ctatttcgtc
     5461 aacctgtcgc gagtctcgat ctccgaccag cactggatac agcgcttcac cgcgatgctg
     5521 cgcgagagcg gcgtcaaccc caatcagctg gtgttcgaaa tcacggaaac ggcggcgatg
     5581 tcggaaatcg acgtcacgct caccttcatc aagcggctga aggagatggg ctgccgcttt
     5641 gcgctggacg atttcggcgc cggcttcagt tcgttctact atctcaagcg cttcgacgtc
     5701 gattatctca agatcgacgg cagcttcatc cgcgacctcg ccaccgacga gggcagccgc
     5761 attttcgtgc gggcgctgaa cgacgtcgcg cacggcctcg acaagcaggt catcgcggaa
     5821 tgggtggaaa ccccggaggt gcagaccctg ctccttggca tgggcaccca gttcggccag
     5881 ggctacctgt tccagcggcc gaccctgctc gagcacgccg ccaagccggc cctcgacaac
     5941 c
//
LOCUS       DBNGSG010000377         5404 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4707683, whole genome shotgun sequence.
ACCESSION   DBNGSG010000377 DBNGSG010000000
VERSION     DBNGSG010000377.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5404)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5404)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5404
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4707683"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="AC158Q_09755"
     CDS             <1..278
                     /locus_tag="AC158Q_09755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR3340102.1"
                     /translation="KEEMFALTSQMRRAAISIPSNIAEGAARNTDKEFLQFLTIARGS
                     LSELETQVIISRNLGYSKDTTGIYSNIDNLFGLMGGLIKSIQRRNSK"
     gene            374..1345
                     /locus_tag="AC158Q_09760"
     CDS             374..1345
                     /locus_tag="AC158Q_09760"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase family protein"
                     /protein_id="HIR3340103.1"
                     /translation="MRPESRIYVAGHRGLAGSALVRRLEGRGYRNLVLRTHAELELAD
                     RAAVRALFEKEHPEHVYLAAAKVGGIMANFELPADFVYLNLTIQNNVIEEARRAGVKR
                     LLFLGSVCIYPRDCPQPIREEHLLTGPLEFTNRPYAIAKIAGIEMCWAYNRQYGTRYL
                     AAMPNNLYGPGDNYDLRHSHVLPAFIRKFHEAKAGGAKSVTLWGTGTPRREMLHSDDM
                     AEACVRLMELPDDDFARIVGTDPAAPQPPLINIGWGEDYTIRELAQRVRETVGFQGAI
                     EWDAAKPDGTPRKLLDVSRMKALGWSPRVTLDAGLRQAYADFLSRFG"
     gene            complement(1589..3007)
                     /locus_tag="AC158Q_09765"
     CDS             complement(1589..3007)
                     /locus_tag="AC158Q_09765"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019042080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HIR3340104.1"
                     /translation="MKLIPTILCGGAGSRLWPVSREMHPKSFIRLADGQSLLQKSYLR
                     AAALPSVSEIVTVTNHGLYFKVLDDYRESGQSGIADTFVLEPFGRNTAPAIAAAALRI
                     AGVHGEDALMLVLTADHLISDQPAFAKAVAQAVRLAQSGKIATFGIRADAPETAYGYI
                     EFEGSRVIRFVEKPSLDKARGYVESGKYLWNSGMFCFSAGTILREMRRHCPDIVAGVA
                     ACLERSRVTGGAQAPVVELDVERFEKVPELSIDYAVMEKSGEVAVVPCDIGWSDVGSW
                     NSLGALVPPDAAGNRVEGDAMLHDVSNCYIRSEGRMVGALGVDNLMVIDTPDALLIAD
                     RGRAQDVKNLYAELKTRGHESHKLHRTVHRPWGTYTVLEEGKGFKIKRIEVKPGASLS
                     LQMHRHRSEHWVVVSGTARIVNGENELDIRANESTFIPAGNRHRLSNPGAADLVIIEV
                     QAGSYLGEDDIVRFDDVYGRVK"
     gene            complement(3097..3981)
                     /gene="rfbD"
                     /locus_tag="AC158Q_09770"
     CDS             complement(3097..3981)
                     /gene="rfbD"
                     /locus_tag="AC158Q_09770"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007589056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HIR3340105.1"
                     /translation="MTTILVTGKDGQVGWELQRSLAPLGSIIALDRKGMDLSSPDSIR
                     RAVAAAKPGIIVNAAAFTAVDKAESEAALAMQINGAAPGIMADEAKRLGALLVHYSTD
                     YVFDGTKNGPYTEHDRPCPINAYGRSKLAGEEAVRDSGASHLIFRTSWVYAARGSNFV
                     RTMLRLARERPELRIVSDQVGAPTSARSIAEMTAQVLKDAARAGKNSGLYHFTAAGAV
                     SWFGFAEAIFAETARRFADFKAPRLVPIPASEYPTPARRPANSRLDCSKLTATFNIAP
                     PSWEAALRQCLGELENSE"
     gene            complement(3978..4523)
                     /gene="rfbC"
                     /locus_tag="AC158Q_09775"
     CDS             complement(3978..4523)
                     /gene="rfbC"
                     /locus_tag="AC158Q_09775"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HIR3340106.1"
                     /translation="MNVIKTDIPDVLIFEPKVFGDARGFFLESFNQRVFREQTGLDPR
                     FVQDNHSRSGRNVLRGMHYQVRQPQGKLVRVVAGEVFDVAVDLRRRSVTFGKWAGTTL
                     SAENKRQMWIPEGFAHGFLVLSDTADFLYKTTDYYAPEHERCVIWNDPDIGVRWPLNG
                     SPLLAAKDQAGKRLKDAETFP"
     gene            complement(4650..>5404)
                     /locus_tag="AC158Q_09780"
     CDS             complement(4650..>5404)
                     /locus_tag="AC158Q_09780"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3340107.1"
                     /translation="ILGTEIVARATPDGYTLLLGNISMAFNAALYKKLPFDALRDLIP
                     VSLVTDQPNILVANPALPAKSFKEFTTLARSQPGKLTFGSAGIGSGTHLAMEMLLMSQ
                     NMSLVHVPYKGTGPALTALLGNQIAVFFSTFASALPHVKGERLRAFAVTSAKRTRALP
                     EVPTVAESGVPGYEYSTWYGLLAPAGTPRARVDQLNKATVAVLNTPETRRKYENQGVD
                     AIPSTPARYAAYLKSETEKWTKVVRAANIPLQ"
BASE COUNT         1002 a         1709 c         1713 g          980 t
ORIGIN      
        1 caaaggaaga aatgtttgct ctcacgagcc agatgcgacg agcggcgatt tccattccca
       61 gcaatatcgc ggaaggcgcg gccagaaaca ccgacaagga attcctgcaa ttcctcacca
      121 tagccagagg ctctctcagt gagctcgaaa cccaggtcat catcagccgt aatctcggtt
      181 attcgaagga cacgaccggg atatactcga atatagataa cttgtttggc ctgatgggcg
      241 gcctcatcaa gagcatccag cgccgcaaca gcaagtaacc ggactcctcg cgtcaaacat
      301 aagcaaatcg cctcactcct cacccctcac cgtaacaatg gcctcctcac ccctcactcc
      361 tcactcctca cccgtgcgtc cggagagcag gatctacgtt gcgggacacc gcggcctggc
      421 cggctccgcg ctggtccgcc ggctcgaggg cagaggttac cgcaacctgg tcctgcgcac
      481 gcatgcggag ctggagctcg ccgaccgcgc cgcggtgagg gcgttattcg aaaaagagca
      541 tcccgagcat gtctacctgg cggccgccaa ggtcggcggc atcatggcca acttcgagct
      601 tcccgccgat ttcgtttacc tgaacctcac gatccagaac aacgtcatcg aggaggcgcg
      661 ccgcgcaggc gtgaagcggc tgctgttcct gggctcggtc tgtatttacc cgcgtgactg
      721 cccccagccg atccgcgaag agcacctgct gaccggcccg ctcgagttca ccaaccgccc
      781 ttacgccatc gccaagatcg cgggcatcga gatgtgctgg gcctacaacc ggcagtacgg
      841 cacacgctat cttgccgcga tgccgaacaa cctctacggt ccgggcgata actacgatct
      901 gcggcattcg cacgtcctgc ccgcgttcat ccgcaagttc cacgaggcga aggccggcgg
      961 ggcgaagtcg gtgactctgt ggggcaccgg caccccgaga cgggagatgc tccacagcga
     1021 cgacatggcc gaagcctgcg tcaggttgat ggagttgccg gacgacgatt tcgccaggat
     1081 cgtcggcacc gatcccgccg cgccgcagcc gccgctgatc aatatcggct ggggcgagga
     1141 ctacacgatc cgggagctcg cgcaacgcgt gcgcgaaacg gtcgggttcc agggcgcaat
     1201 cgaatgggac gcggccaagc ccgacggcac gccgcgcaag ctcctcgacg tgagccgcat
     1261 gaaggcgctc ggctggtcgc cgcgcgtcac gctggatgcc gggctgcggc aggcctacgc
     1321 ggattttctg tcccgcttcg gttaatagac aggattaacg gtacaagacc cgctaaaccc
     1381 gttaaaagca tcaatagaca ggattaacga aaccacaaac ccgttaaccc cacgaataga
     1441 caggatttac atgattaaca agattagaac gaacaccgat tcagaatcag gttcttgttt
     1501 ttgattgaat cttgtaaatc ctgttaatcc tgtccaataa ggatcttgac gggtttaacg
     1561 ggttttcgat tgaccctgtg aatcctgtct atttcacgcg gccgtaaacg tcgtcgaacc
     1621 tgacgatgtc gtcctcgccc aggtaactgc cggcctggac ttcgatgatg acgagatccg
     1681 cggccccggg gttggacagc cggtgcctgt tgcccgccgg aatgaacgtg gattcgttcg
     1741 cccggatgtc cagttcattc tcgccgttga cgatccgggc cgtgccgctg acgacgaccc
     1801 agtgctcgct gcgatgccgg tgcatctgca gcgagagcga ggcgcccggc ttgacttcga
     1861 tgcgcttgat cttgaagccc ttgccttctt ccagcacggt gtaggtgccc cacggccggt
     1921 gcacggtgcg atggagcttg tgcgattcgt gcccccgggt cttcagctcg gcgtagaggt
     1981 tcttgacgtc ctgggcgcgg ccgcgatcgg caatgagcag cgcgtcgggc gtgtcgatga
     2041 ccatgaggtt gtccacgccc agcgcgccga ccatgcggcc ctcgctgcgg atatagcagt
     2101 tggacacgtc gtgcagcatc gcgtcgccct cgacgcggtt gccggccgcg tcgggcggca
     2161 ccagggcgcc cagcgaattc caggaaccga cgtcgctcca gccgatgtcg cacggcacga
     2221 ccgcgacttc gccggacttt tccatcaccg cgtaatcgat cgaaagctcg gggactttct
     2281 cgaatcgttc aacgtccagc tcgacgaccg gcgcctgcgc gccgcccgtg acgcgcgagc
     2341 gctcgaggca ggcggcaact cccgcgacga tgtccgggca gtgccgccgc atctcgcgca
     2401 gtatcgtgcc ggcggagaag cagaacatgc cggagttcca cagatacttt cccgattcga
     2461 cgtagccgcg cgccttgtcg agggacggct tctcgacgaa ccggatgacc cggctgcctt
     2521 cgaactcgat atagccgtaa gcggtctccg gcgcgtcggc acggatcccg aaagtcgcga
     2581 ttttccccga ttgggcgagc cgcacggcct gcgcgaccgc ttttgcgaac gccggctgat
     2641 cggaaatcag gtggtccgcc gtcagcacca gcatcaacgc gtcctcgccg tgcacgccgg
     2701 cgatgcgcag cgcggcggcg gcgatggccg gggcggtgtt gcgcccgaac ggctcgagca
     2761 cgaacgtgtc tgcaatcccg gattggcccg actcccggta atcgtccagt accttgaagt
     2821 agagcccgtg attggtgacg gtgacgatct cggagaccga cggcaacgcg gccgcgcgca
     2881 gatacgattt ctgcagcagg ctttgcccgt cggcaaggcg gatgaacgat ttcgggtgca
     2941 tctcgcgcga aaccggccac aggcgggagc ccgcgccgcc gcaaaggatg gtgggtatca
     3001 gcttcattcg tgaatcgtat gtcgggaacc gggaataggg aataggaaac aggaaacagg
     3061 gaaggcggcg cagggtgcgc cagcaaagag gcccctctat tcgctatttt ccagttcccc
     3121 gaggcattgc cgaagcgcgg cttcccacga cggcggcgcg atgttgaagg tggccgtgag
     3181 cttcgagcaa tcgagccgcg agttggccgg ccggcgggcg ggcgtcgggt attcggaagc
     3241 ggggatcggg accagccgcg gcgccttgaa atccgcgaac cggcgcgccg tctcggcgaa
     3301 gatcgcctcg gcaaagccga accatgacac ggcgccggcg gccgtgaagt gatagagccc
     3361 gctgtttttc cccgcccgcg cggcatcctt cagcacctgc gccgtcattt cggcgatgga
     3421 cctggccgaa gtgggcgcgc cgacctggtc gctgacgatc ctcaattcag gccgctcgcg
     3481 cgcgagccgc agcatcgtgc ggacaaaatt gctgccccgg gctgcataga cccagctcgt
     3541 gcggaaaatc aggtgtgacg cgccggagtc ccggacagcc tcttcgccgg ccagcttgct
     3601 gcgcccgtag gcgttgatgg ggcaaggacg atcgtgctcg gtgtaagggc cgttcttggt
     3661 gccgtcaaac acgtagtcgg tcgagtagtg aaccagcaat gcgccgaggc gtttcgcctc
     3721 gtccgccatg atgccgggcg cggcgccgtt gatctgcatg gccagcgcgg cttccgattc
     3781 cgccttgtcc accgccgtga acgcggcggc attgacgatg atcccgggtt ttgccgcggc
     3841 gaccgcgcgg cgtatggagt ccgggctgga gagatccatg cccttgcggt cgagcgcgat
     3901 gatgctgccg agcggcgcca gcgagcgctg caactcccat ccgacctggc cgtccttacc
     3961 cgtgacaaga atcgtcgtca cggaaaggtt tccgcgtcct tcagccgctt gccggcctga
     4021 tccttggccg cgaggagggg gctcccgttc agcggccatc gcacgccgat atcgggatcg
     4081 ttccagatga cgcagcgctc gtgctcgggc gcgtagtagt cggtggtctt gtagagaaag
     4141 tccgcggtgt cggagagaac caggaagccg tgggcgaagc cctccgggat ccacatctgg
     4201 cgcttgttct cggcggagag cgtggtgccc gcccacttgc cgaaggtcac ggagcgcctc
     4261 cgcaggtcga ccgcgacgtc gaatacttcg cccgcgacaa cccggaccag cttgccctgc
     4321 ggctgcctga cctggtagtg catcccgcgc agcacgttcc tgcccgagcg cgagtgattg
     4381 tcctggacga aacgcggatc gagcccggtt tgctcccgaa aaacacgctg gttgaagctc
     4441 tcgaggaaaa aaccgcgcgc gtcgccgaag accttcggct cgaaaatcag cacgtccgga
     4501 atatcggtct tgatgacgtt catcacgaat gcagtttcaa gtcacaagat acaagttaca
     4561 aggaaaagcg aacgggcctg cgccctgctt tttgaatatt gaacgttgta ccttgtacct
     4621 tgcaacttgc aacttgaacc cgcgtagcgt cattgcagcg ggatattcgc cgcccgcacc
     4681 accttggtcc atttttcggt ttcggacttg aggtaagcgg cgtagcgcgc cggagtcgag
     4741 ggaatcgcat ccacgccctg gttctcgtac ttgcggcgcg tctccggcgt gttgagcacg
     4801 gctaccgtgg ccttgttcag ctggtcgacg cgcgcgcgcg gcgtgccggc cggcgcgagc
     4861 aacccgtacc aggtgctgta ctcgtagccg ggcacgcccg actcggcgac cgtgggcact
     4921 tcgggcagcg cgcgggtgcg cttggcgctc gtcacggcaa aggcgcgcag ccgctcgccc
     4981 ttgacgtgcg gcagggcaga ggcgaaggtg gaaaagaaca ccgcgatctg gttgccgagc
     5041 agcgccgtca acgccgggcc ggtgcccttg tagggtacgt gaaccaggct catgttctgg
     5101 gacatgagca gcatttccat tgccaggtgc gtgcccgagc cgatgccggc ggaaccgaat
     5161 gtgagcttgc ccggctgcga gcgcgcgagc gtggtgaatt ccttgaacga ctttgcgggc
     5221 agcgcgggat tcgcgaccag gatgttcggc tggtcggtca ccagcgagac cgggattaaa
     5281 tcccgcagcg cgtcgaacgg cagcttcttg tagagggcgg cgttgaacgc catgctgata
     5341 ttgccgagca gcagcgtgta gccgtccggc gtggccctgg cgacgatttc ggttccgagg
     5401 atgc
//
LOCUS       DBNGSG010000378         6109 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3008014, whole genome shotgun sequence.
ACCESSION   DBNGSG010000378 DBNGSG010000000
VERSION     DBNGSG010000378.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6109)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6109)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6109
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3008014"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..1045)
                     /gene="glyQ"
                     /locus_tag="AC158Q_09785"
     CDS             complement(158..1045)
                     /gene="glyQ"
                     /locus_tag="AC158Q_09785"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HIR3340108.1"
                     /translation="MLTFQQIILRLNQYWDKQGCVLLQPYDMEMGAGTFHTATFLRAI
                     GPEPWNAAYVQPCRRPKDGRYGENPNRLQHYYQYKVVMKPSPEKFQDLYLGSLTDALG
                     IDPKKNDIRFVEDDWESPTLGASGLGWEVWLNGMEITQYTYFQQAGGLDCKPVLGELT
                     YGLERLAMYLQGKDSVFDLVWVDGVTYGDVYHQNEVEQSAYNFEHANVQMLFEHFNQF
                     EREARRLIEGGLPLPGYEMVMKCSHTFNLLDARGAISTTERAAYIGRVRALARLVAQA
                     YYDSREALGFPMLKQREPA"
     gene            complement(1090..2754)
                     /gene="lnt"
                     /locus_tag="AC158Q_09790"
     CDS             complement(1090..2754)
                     /gene="lnt"
                     /locus_tag="AC158Q_09790"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HIR3340109.1"
                     /translation="MQNDECRVVKDDTESHVGRSRSFSLINHHSSLITAFSLGAATVA
                     GYAPFYLFPLPVITLAGLFYLWSRAPGPRAAAWIGFAFGLGLFLFGVSWVYVSLHDFG
                     AMPMPLAAAATALFCAFLALFPALVGHASTRFKMSNTFRFGVLAPAAWVLAEWIRSWI
                     FTGFPWLALGYSQVPWSPLAGYAPVLGIHGVTLATAVSAGLVVVLWLRYAERDEGGGR
                     RAEEEKANDARPLIPHPSSLIPRGILHPSAFILLALWAGGFGLKQVEWTQPVGAPINI
                     SVLQGNVPQDLKWLEERVPVTLETYSALVATSSSPLIILPETALPLFIDQVPQDYLAM
                     LAGHARKNGGDILIGVPERLPSGEYFNSVVSLGTAPSQTYRKSHLVPFGEFIPLRPVL
                     GWIVSVLAIPLQDFSRGAESQRPLAVAGQRVAVNICYEDAFGEEIIRQLPEATLLVNV
                     SNVAWFGRSIAPGQHLQISQARALETGRFMLRATNTGVTGVIGPDGRIAAAAPEFTLT
                     AVTHRVQGYGGSTPYVRWGNYAALVLCLIMIAVAAGAGRRNSRSAA"
     gene            complement(2758..3588)
                     /locus_tag="AC158Q_09795"
     CDS             complement(2758..3588)
                     /locus_tag="AC158Q_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HIR3340110.1"
                     /translation="MPDSPINKPTLLERVGAWLSREPEDREELIELLRAAYNRNLIDA
                     EALSMIEGVLQVSEMQARDIMVPRAQMDVIDIRESPDQFVPMIMQTGHSRFPVIGENK
                     DDVIGILLAKDLLRHYAGEEEFNVRDMLRPAVFIPGSKPLNVLLKEFRKNRNHIAIVV
                     DEYGGVAGMVTMEDVIEQIVGDIEDEYDFDDVEDNIVPEKGGQYRVKALTEIPDFNTA
                     FGTRYSNQEFDTIGGLVMSRFGRVPKRGEQISFDNLAFKVLRADSRRLHLLQVTKKKQ
                     "
     gene            complement(3682..4146)
                     /gene="ybeY"
                     /locus_tag="AC158Q_09800"
     CDS             complement(3682..4146)
                     /gene="ybeY"
                     /locus_tag="AC158Q_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HIR3340111.1"
                     /translation="MSERFASHDPRLRLSVQYAVRARGLPSRAQLRRWARAALERDAR
                     VTVRIVSGTEARTLNHHFRGKRHPTNVLTFVMRERPRLEGDLALCAPVIAREARAQHK
                     SAAAHYAHLVVHGVLHLQGYDHERAREAREMERRETQIVTRLGYPDPYKDQG"
     gene            complement(4143..5123)
                     /locus_tag="AC158Q_09805"
     CDS             complement(4143..5123)
                     /locus_tag="AC158Q_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003261665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIR3340112.1"
                     /translation="MKPVELAFKPVDNQRLANLCGALDENLRQIETALDVSIARRGER
                     FAVTGKPGQARIAAQVLQSFYERAKNELSLEDVQLGLIEAAGPRKTPAPDGSPALVTR
                     RPDLHGRTPRQVQYLQNILAHDLTFSIGPAGTGKTYLAVACAVDALERDSVKRIVLVR
                     PAVEAGERLGFLPGDMVAKVDPYLRPLYDALYDLMGFDKVAKLFDRSAIEIAPLAFMR
                     GRTLNHSFVILDEAQNTTPEQMKMFLTRIGFGSKAVVTGDVTQIDLPRGQKSGLVEAR
                     DVLKKVRGIAFTHFESEDVVRHPLVQRIVNAYENYVSPTASRGPETGGRT"
     gene            complement(5120..>6109)
                     /gene="miaB"
                     /locus_tag="AC158Q_09810"
     CDS             complement(5120..>6109)
                     /gene="miaB"
                     /locus_tag="AC158Q_09810"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HIR3340113.1"
                     /translation="IEARRATGKPQVDVSFPAIEKFDNLPPARVEGAAAFLSVMEGCS
                     KYCSFCVVPYTRGEEVSRPFEDVLTEAAELAQQGVREITLLGQNVNAYRGDADGRAAD
                     LALLLEYVAAIPGIERLRYTTSHPKEFTQRLVEAHAGIPQLAPHVHLPVQAGADRILA
                     AMKRGYTVLEYKSIVRRLRARRPDISITSDFIVGFPGETEADFEATMRLIEEIGFDAS
                     FSFLYSRRPGTPAANLPDDTPHEVKLARLRRLQARIELQAQALSRGMVGTRQRVLVEG
                     ASRKNTAELCGRTANNRVVNFPGPWNLVGRFADVTVTTALSHSLRGELAPALV"
BASE COUNT         1057 a         1940 c         2021 g         1091 t
ORIGIN      
        1 ggggtggcga gcacgtcctg cgggggcgcg ctgtcagcgg cgacgaaggc cgcctcgacc
       61 agacccctgt gtatctcgcg cgcaaaggtg gtgcccagcg cgtgcagcgc cctgggcggg
      121 agctcctcgg tgagcagctc caccagcagg gtctggctca cgccggttcc ctttgcttca
      181 gcatagggaa gcccagcgct tcgcgcgagt cgtagtaggc ctgcgccacc agccgtgcga
      241 gcgcgcgcac gcggccgatg taggcggcgc gctcggtggt ggagatggcg ccgcgcgcat
      301 ccagcaggtt gaaggtgtgc gagcacttca tcaccatctc gtagccgggc agcggcagac
      361 cgccctcgat caggcgcctg gcctcgcgct cgaactggtt gaagtgctcg aacagcatct
      421 gcacgttggc gtgctcgaag ttgtaggcgg actgctcgac ctcgttctgg tgatagacgt
      481 cgccataagt cacgccgtcc acccagacga ggtcgaacac gctgtccttg ccctgcaggt
      541 acatcgcgag gcgctcgagg ccgtaggtga gttcgcccag caccggcttg cagtcgagcc
      601 cgccggcctg ctggaagtag gtgtactgcg tgatttccat gccgttcagc cacacctccc
      661 agcccagccc tgacgcgccc agcgtcggcg attcccagtc gtcttcgacg aagcggatgt
      721 cgttcttttt cgggtcgatg ccgagcgcgt ccgttaacga gccgagatag aggtcctgga
      781 atttttccgg ggagggcttc atcaccacct tgtactggta gtaatgctgc aggcggttgg
      841 gattttcgcc gtagcgcccg tccttgggcc ggcggcaggg ctggacgtac gccgcgttcc
      901 acggttccgg cccgatcgcg cgcagaaaag tcgctgtgtg aaacgtgccc gcacccatct
      961 ccatgtcata cggctgcagc aggacgcagc cttgcttgtc ccaatactgg ttgagccgga
     1021 ggatgatttg ctggaaggtc agcatgattc gcgggcacgc tcgtaaccca cgaatattac
     1081 aggcattcgt caggctgcgg aacgagagtt gcggcgcccg gctccggcgg cgaccgcgat
     1141 catgatcagg cagagcacaa gcgccgcgta gttaccccat cgcacgtagg gcgtggaacc
     1201 gccatatccc tggacccgat gggtaacggc agtcagtgtg aactccggcg cggcggcagc
     1261 gatccgcccg tcaggaccga tcacgccggt gacgccggta ttggtcgcgc gcagcataaa
     1321 acgcccggtc tccagcgcgc gtgcctggga aatctgcagg tgctgcccgg gggcgatcga
     1381 gcggccgaac caggccacgt tactgacgtt gacgagcagc gtcgcctctg gcagttggcg
     1441 gatgatctct tccccgaaag cgtcctcgta gcagatattg accgcaacgc gttgcccggc
     1501 cacggcgagc gggcgctggc tctccgcgcc gcgcgaaaag tcctgcagcg gtattgcgag
     1561 cacgctcacg atccagccca gcacggggcg caacggaatg aactcgccga acggcaccag
     1621 atgcgacttg cggtaggtct ggctgggcgc ggtaccgagg gaaaccacgc tgttgaaata
     1681 ctcgccgctg ggcaggcgct ccggcacgcc gatcaggatg tcgccgccgt ttttgcgcgc
     1741 gtgaccagcc agcatcgcga gatagtcctg cgggacctgg tcgataaaca gcggcagcgc
     1801 ggtctcgggc aggatgatga gcgggctcga cgaagtcgca acaagagcgg aataggtctc
     1861 cagggtgacc ggcacccgct cttccagcca cttcaggtcc tgcgggacat tgccctgcaa
     1921 cacgctgata ttgatgggcg caccgacggg ctgggtccac tcgacctgct tcaggccgaa
     1981 gccgcccgcc cagagggcga gcaggatgaa ggcagaagga tggaggatgc cccgaggaat
     2041 gagggacgag ggatgaggga tgaggggtct cgcgtcgttc gccttttctt cctccgccct
     2101 ccgccctccg ccctcatccc tttcggcgta acgcagccac agcacgacga cgagccctgc
     2161 ggatactgct gtggcgagcg tgacgccgtg aatgcccagc accggcgcat aacccgcgag
     2221 cgggctccac ggaacctgcg aatatcccaa cgcgagccag ggaaaaccgg tgaatatcca
     2281 gctgcgaatc cattccgcca gtacccaggc tgccggcgcg agcactccga accggaaggt
     2341 gttcgacatt ttgaaccggg tggaggcatg gcccaccagt gcggggaaga gtgccaggaa
     2401 ggcgcagaac agggcagtgg cagccgccgc gagcggcatc ggcatggcgc cgaaatcgtg
     2461 caggctcacg tagacccacg agacaccgaa caggaacagc ccgagcccga aggcaaaacc
     2521 gatccacgcc gcggcgcgcg ggcccggtgc gcgcgaccac agataaaaca gcccggccag
     2581 cgtgatcacc ggcagcggga agagatagaa cggggcgtaa ccggctacgg ttgcggcacc
     2641 gagggaaaac gcagttatga gtgatgagtg atgattgatg agtgagaaac tgcgggaacg
     2701 gcccacatgg ctttctgtgt cgtccttcac cactctgcac tcatcattct gcattcatca
     2761 ttgttttttc ttggtgactt gcagcaggtg cagccgccgg ctgtcggcac gcagcacctt
     2821 gaaggcgaga ttgtcgaaac tgatctgctc gccgcgcttg ggcacgcggc cgaagcgcga
     2881 catgacgaga cccccgatgg tgtcgaattc ctggttgctg tatcgggtgc cgaaagcggt
     2941 gttgaaatcg ggaatctcgg tcagcgcctt cacgcggtac tgtccgccct tctcgggcac
     3001 gatgttgtcc tcgacgtcgt cgaagtcgta ctcgtcctcg atgtcgccga cgatctgctc
     3061 gatcacgtcc tccatcgtca ccattcccgc cacgccgccg tactcgtcga ccacgatcgc
     3121 gatgtggttg cggttcttgc ggaactcctt gagaagcacg ttgagcggct tcgaccccgg
     3181 gatgaacacc gccgggcgca gcatgtcgcg gacgttgaat tcctcctcgc cggcatagtg
     3241 gcgcagcagg tccttggcga gaaggatgcc gatcacgtcg tccttgttct cgccgatcac
     3301 cggaaaacgc gagtgcccgg tctgcatgat catcggaacg aactggtcag gcgattcacg
     3361 gatatcgatg acatccatct gcgcgcgcgg caccatgatg tcgcgcgcct gcatttccga
     3421 gacctgcagg acgccctcga tcatggagag cgcctcggcg tcgatcaggt tgcgattgta
     3481 ggcggcgcgc agcagctcga tgagctcctc gcggtcctcg ggttcgcgcg agagccaggc
     3541 gccgacgcgc tcgagcagcg tgggcttgtt gatggggctg tcaggcatgg gaccataagg
     3601 caaaaggcaa aaggcaaaag gcaagagtag cgtagccgcg cggccatgcc gtttttacct
     3661 tttccctttt ccctttttcc ttcatccttg atccttgtag ggatcagggt agccgagccg
     3721 ggtcacgatc tgcgtttccc gccgctccat ttcccgcgct tcccgcgcgc gttcgtgatc
     3781 gtatccctgc aagtgcagca cgccgtggac cacgagatgg gcgtaatgcg ccgctgcgct
     3841 tttgtgctgg gcgcgagcct cgcgtgcaat gaccggggcg cacaacgcca ggtcgccctc
     3901 gagccgcggc cgctcgcgca tcacgaaggt cagcacgttg gtcgggtgcc tcttgccccg
     3961 gaaatggtgg ttgagcgtgc gcgcctcggt tccgctgacg atgcgaacgg tgactcgcgc
     4021 gtcgcgctcg agcgccgcgc gggcccagcg cctcaactgg gcgcgcgacg gaaggccgcg
     4081 cgcgcggacc gcgtattgca cgctcagcct caggcggggg tcatggctgg cgaaccgttc
     4141 gctcatgttc tgccaccggt ctccggcccg cggctggccg tcggcgaaac gtaattttca
     4201 taggcattga cgatgcgctg caccagcgga tggcgcacca cgtcctcgga ttcgaagtgg
     4261 gtgaatgcga tgccgcgcac tttcttcagg acgtcgcggg cttcgacgag tccgcttttc
     4321 tgtccccgcg gcaggtcgat ctgggtgacg tctcccgtca ccaccgcctt gctgccgaag
     4381 ccgatccgcg tcaggaacat cttcatctgc tccggcgtgg tgttctgcgc ctcgtcgagg
     4441 atcacgaagg aatgattgag cgtgcgcccg cgcatgaagg caagcggcgc gatctcgatc
     4501 gcgctgcgat cgaagagctt cgccacctta tcgaacccca tcaggtcgta gagcgcgtcg
     4561 tagagcgggc ggagataggg gtcgaccttg gccaccatgt caccggggag aaagccgagc
     4621 cgctcaccgg cttccaccgc ggggcgcacc agcacgatgc gcttcaccga atcgcgttca
     4681 agcgcgtcca ccgcgcacgc caccgcgaga taggtcttgc cggtgcccgc cgggccgatg
     4741 ctgaaggtga ggtcgtgggc taggatgttc tgcaggtact gcacctggcg cggagtgcgc
     4801 ccgtggaggt ccgggcgccg cgtgaccagc gccggactgc cgtccggcgc aggagtcttg
     4861 cgcggcccgg ccgcctcgat caggccgagc tgcacgtcct cgagcgacaa ctcgtttttg
     4921 gcgcgctcgt agaaagactg cagtacctgt gccgcaatcc gggcttgccc gggcttgccg
     4981 gtgaccgcga agcgctcgcc gcggcgggcg atgctgacat cgagcgcggt ctcgatctgc
     5041 cgcaggtttt catcgagcgc gccgcacagg ttggcgagac gctggttgtc caccggcttg
     5101 aatgcgagtt cgacgggctt caaacgagtg cgggagccag ttcaccgcgc agcgaatggg
     5161 acagcgcggt agtgaccgtg acgtcggcga accggccgac caggttccac gggcccggga
     5221 agttgaccac gcggttgttg gcggtgcggc cgcacagctc ggcggtgttt ttcctggacg
     5281 cgccttcgac cagaacgcgt tgccgcgtgc cgaccatgcc gcggctcagt gcctgggctt
     5341 gcagctcaat tcttgcctgc agccgccgca accgcgcaag ctttacctcg tgcggggtat
     5401 cgtcgggcaa gttggccgcc ggcgtgccgg ggcgcctgct gtagaggaag ctgaagctgg
     5461 cgtcgaaacc gatttcttcg atcagcctca tcgtcgcctc gaagtcggcc tcggtttcac
     5521 cggggaaccc gacgatgaaa tctgatgtga tcgagatgtc ggggcgccgc gcgcgcagcc
     5581 ggcgcacgat ggacttgtac tcgaggacgg tatagccgcg cttcatggct gcgagtatgc
     5641 ggtcagcccc cgcctgaacc ggcagatgca cgtgcggcgc cagctgggga atgccggcat
     5701 gagcctcgac caggcgctgg gtgaattcct tcgggtgaga cgtggtgtag cgcagccgct
     5761 cgatgccggg gatcgccgcc acgtattcga gcagcagggc cagatcggcg gctcttccgt
     5821 ccgcgtcacc gcgataggcg ttcacgttct ggccgagcag ggtgatttcc ctgacgccct
     5881 gctgggccag ctccgcggcc tcggtcagga catcctcgaa ggggcgcgac acttcctcgc
     5941 cgcgggtgta cggcaccacg cagaaactgc agtacttgct gcagccttcc atgaccgaaa
     6001 ggaaagcggc ggcgccttcg acgcgcgcgg gcggcaggtt gtcgaatttc tcgatggccg
     6061 ggaatgacac gtccacctgc ggcttgccgg tcgcgcggcg cgcctcgat
//
LOCUS       DBNGSG010000379         4041 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2499545, whole genome shotgun sequence.
ACCESSION   DBNGSG010000379 DBNGSG010000000
VERSION     DBNGSG010000379.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4041)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2499545"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1732
                     /locus_tag="AC158Q_09815"
     CDS             <1..1732
                     /locus_tag="AC158Q_09815"
                     /inference="COORDINATES: protein motif:HMM:NF015649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="HIR3340114.1"
                     /translation="LRDPGNDALDRWLARAEPPAGTNDAQAGSGILLELALGRAMIAV
                     FRGDFAASEAIIGILRPMTEAQQADARILTGMASAVGHLLDGSNAEALRTARECLGFA
                     ESEGIHPYDEWLRVMAAVAALGADDRDTARGELQALEASGARLRRGGQVFIHYLRGWL
                     ATLEDDAANAGRETKMALAGAVELGIPWLECLARIAWAQALSSDGDERGAEAQLRSAA
                     AIANQVRSPLLDVSTHLAAAGVANRALDEHSALGSLRKGFALGRKHGLRNIVALRPKF
                     LAEVCALALGKGIETEFARALVRSRNLPPPSAALHVREWPRPFQVCTLGGFSLLRESR
                     PVEFSGKGPGRPVELLKVLVALGGQNVRSDQLADALWPRVDADYAHKSFTATLHRLRR
                     ILDEDDALVLRDARLSLNPRLCWVDTWALDHLVAELDSALREPAARPAGRDVRALAED
                     ALALYRGPFLPDESEQPSYIACREQTRAKLLRCMTRVARRWEETGIPEAAIDCYLRCI
                     DADALCEVYYRNLMLCYHRRGELAEALSTYERLRTVLSVQLKSPPSPETQAVYSSLLA
                     LQVSRAAPRE"
     gene            complement(1740..2150)
                     /locus_tag="AC158Q_09820"
     CDS             complement(1740..2150)
                     /locus_tag="AC158Q_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340115.1"
                     /translation="MRVSLARLGMIVEAQDPVLGYVNVYAPAPLPLSQEEWRQASEAD
                     LPRAREIIGPYVGLYAHFFKFEPEGLEVVIGGTVVEVPTGVAVSYTARMREIAPPKSG
                     IPRREYLPPTAVQIGLDKMWRELQREFDATYRRR"
     gene            complement(2455..>4041)
                     /locus_tag="AC158Q_09825"
     CDS             complement(2455..>4041)
                     /locus_tag="AC158Q_09825"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3340116.1"
                     /translation="GLERFIQEARTLARFDHPNIVRVHSVFEFNGTAYMVMRFEEGEN
                     FGSLLERRGTLPEKDVLRVLLPILDGLELVHNAGFIHRDIKPDNIHIRADGSPVLLDF
                     GSARHALGKARTLTILVAPGYAPFEQYNSNSENQGPWTDIYGLGATCYRAMAGTPPLD
                     AIARSKGILGSTREILVPARTIGNGRYSGQLLAAIDHALEFDEKNRPQTVAEWRREIT
                     EESRPAAAAPVEPERPRSAPVPPAPPSSAAPAQTGATASPAATAAAGWSGARAPIAWA
                     IFGAVAGAIAIALFLQITRREPDPQPPPMPPVEVDKKLAALELEKIRQAQEAEKRRQA
                     DEVEKKRVAEEAGRKRRAEEAEMLRLTQEADRKRLAQEAERKRQAQEAERKRLAQEAE
                     RKRQAQEAEKIRLAQEAERKRLDELERRAQQQKPGSQELLAKAAEADARGEFSSALQI
                     LTPLATGGNATAQVRLAEMYFNGRGVPVNYFQAYVWYSLAVRCGNTAATAGKARTANN
                     LQPAQVNQADTVAGGMKACK"
BASE COUNT          610 a         1390 c         1296 g          745 t
ORIGIN      
        1 cctgcgcgat ccgggcaatg acgccctcga ccgctggctc gcgcgtgccg aaccgccagc
       61 cggaacgaac gatgcgcagg ccggatccgg gattctgctg gaactggcgc tgggccgtgc
      121 catgatcgcg gtttttcgcg gggatttcgc ggcgtcagaa gcgatcatcg gcatcctccg
      181 ccccatgacg gaagcacaac aggcggatgc ccgaatcctg accggcatgg catccgcggt
      241 cggccatttg ctggacggct cgaatgccga ggcgctgcgc acagcacgcg aatgtctcgg
      301 cttcgccgaa tcggaaggca tccatcccta cgatgaatgg ctgcgcgtga tggctgcggt
      361 ggccgcgctg ggtgccgacg accgcgacac cgcccgcggc gagttgcagg cgctggaggc
      421 ctcgggcgcg cgcttgcgac gcgggggcca ggttttcatc cactacctgc ggggctggct
      481 cgccacgctc gaggacgacg ccgcgaacgc cggtcgcgag accaagatgg cactcgcggg
      541 cgccgtcgaa ctggggatac cctggctgga atgcctcgca cgcatcgcct gggcgcaagc
      601 gctttcgtcc gatggcgacg agcgcggcgc ggaagcccag ctgcgcagcg cggcggctat
      661 cgcgaaccag gttcgcagtc ccctgctcga cgtatcgacg cacctggcag cggcgggcgt
      721 agccaatcgc gccctcgatg aacactctgc gctgggctcc ttgcgcaagg ggttcgcgct
      781 gggacgcaag catggattgc ggaacatcgt ggctctgcgc ccgaaattcc tggccgaagt
      841 gtgcgcgctc gcgctgggca aaggcatcga gaccgagttt gcacgcgcgc tggtgcgctc
      901 ccgcaatctt ccgcccccct cggccgcgct gcacgtcagg gagtggcccc gtcccttcca
      961 ggtctgcacg ctgggcgggt tcagcctttt gcgcgagtcc cggcccgtcg agttctcggg
     1021 caaggggccc ggacgcccgg tggaactgct caaggtgctg gtcgcgctgg gcgggcagaa
     1081 cgtgcgcagc gaccagctgg cggacgcgct gtggccgcgc gtggacgcgg attacgccca
     1141 taaatctttc accgcgacgc tgcaccggct gcggcggata ctcgacgagg acgacgcgct
     1201 ggtgctgcgc gatgcgcgcc tgagcctgaa tccccggctg tgctgggtgg acacctgggc
     1261 gctggatcac ctggttgccg aactggacag cgcgttgcgc gagcccgcgg cgcggccggc
     1321 cgggcgtgac gtgcgcgcgc tcgccgagga cgcgctggcc ctctatcgcg ggccgttcct
     1381 gccggacgaa tcggagcagc cgagctacat cgcgtgccgc gagcagacac gcgcgaagct
     1441 gctgcggtgc atgacgcggg tcgcgcgccg ctgggaggaa accggcatac ccgaggccgc
     1501 catcgactgc tacctgcgtt gcatcgacgc ggatgcgttg tgcgaagtct actatcgcaa
     1561 cctgatgctt tgctaccatc gccgcggcga actcgccgaa gcgctctcca cctacgagcg
     1621 tttgcgcacg gtgctgtcgg tgcagttgaa gagcccgccg tcgccggaaa cgcaggcggt
     1681 gtactccagc ctgctcgcgc ttcaggtttc cagggcagca ccgcgcgaat aacgcgaggt
     1741 cagcgcctcc ggtaagttgc gtcgaattcc cgctggagtt cccgccacat cttgtccagg
     1801 ccaatctgga cagcagtcgg cggcaggtac tcccggcgcg ggataccaga cttgggcggc
     1861 gcgatttcac gcatgcgcgc cgtataggaa accgccacgc cggtcggcac ctcgactacc
     1921 gtgccaccga tcaccacttc gaggccttcc ggctcgaatt tgaagaaatg ggcgtacagc
     1981 ccgacgtaag gtccgatgat ctcgcgggcg cgcggcaggt ccgcctccga agcctgccgc
     2041 cactcttcct ggctgagcgg cagcggcgcg ggcgcataga cgttgacgta acccagcacc
     2101 ggatcctgcg cctccacgat cattcccagc cgcgccaggc tcacgcgcat ggcgtcgtag
     2161 gcctcagcac gcgtgacttg gtagacccga gtcccgttct tcttaaggat ggcttcgtaa
     2221 tccgtgcgaa acgcctcgtt aatagcgtct atcgcgcctc ggatgccccc cgtgctcggg
     2281 caacccgcca tgatcagcac tagcaacgca cagcacacga caagcagacg actgctggac
     2341 cctttaccca tacccgtagt catgactccc cctgtcgtgc tctgcgctcg cggcaagccc
     2401 ggttaaaggg cgtgcccttc ttgcttccgc caacaccgca gtgcaagaat tcttctactt
     2461 gcaggccttc atgcccccag ccaccgtgtc ggcttggttg acttgtgccg gttgcaaatt
     2521 gtttgccgtt ctcgctttgc cagccgtggc ggcggtatta ccgcatcgaa ctgccagact
     2581 gtaccagacg tacgcctgaa agtagttcac aggcacgcca cgtccattaa agtacatttc
     2641 ggccagcctc acctgggcag tggcgtttcc ccctgtagca agcggcgtaa gtatctgcag
     2701 ggccgaagaa aactctccac gcgcgtcggc ttccgccgcc ttggcgagca attcctgcga
     2761 tcctggcttc tgctgttgag ctcgacgctc cagttcatcc agccgctttc tttccgcctc
     2821 ctgcgccagc cgtatcttct cggcctcctg cgcttgccgc tttctttccg cctcctgtgc
     2881 cagccgcttc cgttctgcct cttgcgcctg gcgtttgcgt tcagcttcct gcgcgagccg
     2941 cttcctgtcg gcttcctgcg tcagccgcag catttcggcc tcctctgccc gccgtttcct
     3001 gcctgcctct tcagcgactc gcttcttttc aacctcatcc gcctgtcgcc gtttctcggc
     3061 ttcctgcgcc tgtcgtatct tttcgagctc gagcgctgca agcttcttat ccacctctac
     3121 cggaggcata ggcggcggtt gcggatctgg ctcccgtctg gttatttgca gaaataacgc
     3181 aatcgcgatt gccccggcaa ccgccccgaa aatcgcccag gcaatcggcg cgcgcgcgcc
     3241 cgaccagccg gcggctgcgg tggcggcagg cgaagccgtc gcccccgtct gcgctggcgc
     3301 ggcagaagaa ggtggcgcgg gcggcacggg cgcgctgcgg ggccgttccg gctcgacggg
     3361 agccgcggcc gccgggcggc tctcttccgt gatctccctg cgccactcgg ccacggtctg
     3421 cggccggttc ttctcgtcga actccagcgc gtgatcgatg gcggccagca gctggccaga
     3481 gtagcggccg ttgccgattg tcctggccgg cacgaggatc tcgcgcgtgc tgcccaggat
     3541 gcccttgctg cgcgcgatcg cgtcgagggg cggcgtgccg gccatcgcgc ggtagcaggt
     3601 cgcgccgagc ccgtagatgt cggtccacgg tccctggttt tccgaattgc tgttgtactg
     3661 ctcgaacggc gcgtagccgg gggcgacgag gatcgtcagc gtgcgcgcct tgccgagcgc
     3721 gtgccgcgcc gagccgaagt cgagcagcac ggggctgccg tcggcgcgga tgtggatgtt
     3781 gtccggcttg atgtcgcggt gaataaatcc ggcgttgtgg accagttcca gtccgtcgag
     3841 gatcggcagc agcacgcgca gcacgtcctt ctccggcagg gtcccgcgcc gctcgagcaa
     3901 cgacccgaaa ttctcaccct cctcgaaccg catcaccatg tacgcggtgc cgttgaactc
     3961 gaacacggaa tgcacgcgca cgatgttcgg gtggtcgaag cgcgccagtg tgcgcgcttc
     4021 ctggatgaag cgctccagcc c
//
LOCUS       DBNGSG010000380         4186 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3688845, whole genome shotgun sequence.
ACCESSION   DBNGSG010000380 DBNGSG010000000
VERSION     DBNGSG010000380.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4186)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3688845"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="AC158Q_09830"
     CDS             <1..732
                     /locus_tag="AC158Q_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR3340117.1"
                     /translation="DAAADWLATAHARSGVNCSGCHKAEKEGAAAWVRRPDHKVCATC
                     HEPETRGFLAGKHGMRLAEGLSPMTPGRARQPMHAKARSAELGCTSCHSAHRFDTKTA
                     AVEACVGCHRDEHTAAYERSPHYALWKKELAGEAEPGSGVSCASCHLPRDEYRVPGMD
                     AKRVLVQHNQSDNLRPSEKMLRPVCMSCHGLGFATDSLADAKLVRSNFSGKPGVRIKS
                     LDMVAKRVKAIEEGRRQKDAGAKRK"
     gene            767..1327
                     /locus_tag="AC158Q_09835"
     CDS             767..1327
                     /locus_tag="AC158Q_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tll0287-like domain-containing protein"
                     /protein_id="HIR3340118.1"
                     /translation="MKTGKGLWVALIASGVAAAAPAQAQVSYKDMADALHAVMESDRT
                     VYTRMVVNRLQNEEKVIKASEHFKDDKALPLPAQMFRFGAEMVAEKKMGFSYSLQSLW
                     PINKQNMPKTALEKKGLDAVAKDPGKAFYGEETLGKTKYFTAIYADKAIAPACVTCHN
                     EHKDTPKKDFKIGQAMGGVVLRIPMK"
     gene            1377..1772
                     /locus_tag="AC158Q_09840"
     CDS             1377..1772
                     /locus_tag="AC158Q_09840"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3340119.1"
                     /translation="MRFEKLHHRVKDAAPAVVAAAAALVFGSLAVAPAGAQNFPEFPD
                     PRLKQGRAVWMGTCEACHANDVAGAPLVTNKAAWAPRLGKGRDALYRSALGGLTGPKG
                     TQMPPRGGNDKLTDDQVRSAVEYMMAIVK"
     gene            1794..2327
                     /locus_tag="AC158Q_09845"
     CDS             1794..2327
                     /locus_tag="AC158Q_09845"
                     /inference="COORDINATES: protein motif:HMM:NF012344.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR3340120.1"
                     /translation="MQNLAWSLSLVLMAVVAAVFLWVVAGASRAGGDAAAIAQSGFRW
                     RDRLFWIAIAAGIALMFATLWEWPIAGHAAAAPKADVVIRATGHQFRWELDGDTVRAG
                     ELVEFELSSRDVNHGFAIYKDKKHMVAQTQAMPGYVNKLQVRFAEPGEYEVLCLEYCG
                     VAHHGMRAVIKVVGAPK"
     gene            2340..3794
                     /locus_tag="AC158Q_09850"
     CDS             2340..3794
                     /locus_tag="AC158Q_09850"
                     /inference="COORDINATES: protein motif:HMM:NF012343.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HIR3340121.1"
                     /translation="MNAATTRSPDTPSAKLAFQLYAAIGIAVFLLMMIAGAAFRAVQA
                     AWLPIPAQFGYELMTLHGAGMVGVSGLAGAAVMWYFLRRYVKLSTAMFLAMLALSLVG
                     VVMILAAIGIGRFAGAWTFLYPLPAKSAGVWGAHAAAAFVGGLVFIGVGFLLFYLDCA
                     VAILRRYGSLAKALGLDQLFGSGPVDTSLPPTVVASTMVVIINILGILAGAVVLVMTL
                     VNIYVPAIAFDALLMKNLIFFFGHVFINATIYMAVIGVYEIVPRYTGRPWTISRPFLA
                     SWAAVTLIVMAVYPHHLLMDYVMPKWAVVTGQVLSYLSGIPVLLVTVYGALVNIHRSG
                     LKWDLPLRLIVFSLFGWAAGIIPAIIDGVIRVNLVMHNTQWVPGHFHFYLLLGLVPML
                     LGTILYACTRERWAETALDQAAFWTYGVAGVVFCFVFLAGGWQGVPRRFAVHDAAWLG
                     FAQIGTVAATLVLVAAVVLGVRLLARLPRASLSA"
     gene            3791..>4186
                     /locus_tag="AC158Q_09855"
     CDS             3791..>4186
                     /locus_tag="AC158Q_09855"
                     /inference="COORDINATES: protein motif:HMM:NF014667.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR3340122.1"
                     /translation="MSAQRTSIAAALVLAAGLAVTHAATDGFEAFTLESARRLAALRS
                     PVRVPDLALELADGGRARLSAMPGEVLLVDFVYTNCPTVCAALGSVYARLQQRLAPEI
                     AAGGVRLLSVTFDPARDGPGELRASRARFS"
BASE COUNT          658 a         1376 c         1468 g          684 t
ORIGIN      
        1 gatgccgccg ccgactggct cgcgactgcg cacgcgcgct cgggcgtcaa ctgcagcggc
       61 tgtcacaagg cggagaaaga gggcgcggcg gcctgggtga ggcgtccgga ccataaagtc
      121 tgcgcaacct gccacgagcc ggagaccagg ggcttcctcg ccggcaagca cggcatgcgg
      181 cttgccgagg gcctctcccc gatgacgccg ggccgcgcgc gccagccgat gcacgccaag
      241 gcgcgctccg cggagctggg ctgcacgtcc tgtcactcgg cacaccgctt cgacacgaag
      301 acggcggcgg tggaagcctg cgtgggctgc catcgcgacg agcacacggc ggcctacgag
      361 cgctcgccgc attacgcgtt gtggaagaag gaactcgcgg gcgaggccga gccgggcagc
      421 ggcgtctcgt gcgcgagctg ccacctgccg cgcgacgagt accgggtgcc ggggatggac
      481 gcgaagcggg tgctggtgca gcacaaccag agcgacaacc tgcgcccgag cgagaagatg
      541 ctgcggccgg tgtgcatgag ctgccacggg cttggttttg ccaccgattc cctggccgac
      601 gcgaagctcg tgcggagcaa cttctccggc aagccgggcg tgcgcatcaa gagcctcgac
      661 atggtggcga agcgggtgaa ggcaatcgaa gaagggcggc ggcagaagga cgccggcgca
      721 aaaagaaagt agcagcgggg agttcaactt gataaaggag ctttcaatga agacgggcaa
      781 ggggttgtgg gtggcgctga tcgcgtcggg cgttgccgcc gccgcgccgg cgcaggcgca
      841 ggtcagctac aaggacatgg cggacgcgct gcacgcggtg atggagtccg accgcacggt
      901 gtacacgcgc atggtggtga accgcctgca gaacgaggaa aaagtgatca aggcgagcga
      961 gcacttcaag gacgacaagg cgctgccgct gccggcgcag atgttccgct tcggcgcgga
     1021 aatggtggcc gagaagaaga tgggattcag ctactcgctg cagtcgctgt ggccgatcaa
     1081 caagcagaac atgccgaaga cggcgctgga gaagaagggg ctggacgcgg tcgcgaagga
     1141 cccgggcaag gcgttctacg gggaagagac tctgggcaag accaagtact tcaccgccat
     1201 ctacgccgac aaggcgatcg cgcccgcctg cgtcacctgc cacaacgagc acaaggacac
     1261 cccgaagaag gacttcaaga tcgggcaggc gatgggcggc gtggtgctgc gcataccgat
     1321 gaaataggaa attgtgggca ttcctgccca cgatttcccg accggaaccc ggtaacatga
     1381 gattcgaaaa actgcatcat cgtgtcaagg acgcagcgcc ggcggtggtg gcagctgcgg
     1441 cggcgctggt attcggttcc cttgcagtcg cgccggccgg cgcgcagaat ttcccggaat
     1501 ttcccgaccc gcgcctcaag caggggcgcg cagtctggat gggaacctgc gaagcctgcc
     1561 acgcgaacga cgtcgccggc gcgccgctcg tcaccaacaa ggcggcgtgg gcgccgcggc
     1621 tcgggaaagg ccgcgacgcg ctctaccgaa gcgccctcgg cggcctgacc gggccgaagg
     1681 gcacgcagat gccgccgcgc ggcggcaacg acaagctgac cgacgaccag gtcaggtcgg
     1741 cggtcgagta catgatggcc atcgtgaagt agcaccgtac cagggagaaa aaaatgcaaa
     1801 acctagcgtg gagtctgagt ctcgtgctga tggcggtcgt tgccgccgtg ttcctgtggg
     1861 tcgtcgccgg cgcaagccgc gcgggcggcg acgccgcagc catcgcccaa tcgggtttcc
     1921 ggtggcgcga ccgcctgttc tggatcgcga tcgccgcggg catcgcgctg atgttcgcga
     1981 cattgtggga gtggccgatc gccggccacg ccgccgccgc gcccaaggcc gacgtggtga
     2041 tccgcgccac cggtcatcaa ttccgctggg aactcgacgg cgacacggtc cgcgcgggtg
     2101 agctcgtgga gttcgagctg tcgagccgcg acgtcaatca cggtttcgcg atctacaagg
     2161 acaagaagca catggtggcg cagacccagg ccatgcccgg ctacgtcaac aagctgcagg
     2221 tgcgctttgc cgagcccggg gaatacgagg tgctgtgcct cgagtactgc ggggtcgccc
     2281 accacggcat gcgggccgtg atcaaggtcg tcggcgcacc gaaataaagg agcctggaca
     2341 tgaacgctgc cacgacccgc tctcccgaca cgccgtcggc gaagctcgcc ttccagctct
     2401 acgccgcgat cgggatcgcg gttttcctgc tcatgatgat cgccggcgcg gcgttccgcg
     2461 ccgtgcaggc ggcctggctg ccgataccgg cgcagttcgg ctacgagttg atgacgctcc
     2521 acggcgccgg catggtcggc gtctcgggcc tcgccggcgc cgcggtcatg tggtacttcc
     2581 tgcgccgcta cgtcaagctc tcgaccgcga tgttcctcgc gatgctggcg ctgtccctcg
     2641 tcggcgtggt gatgatcctt gccgcgatcg gcatcggccg cttcgccggc gcgtggacct
     2701 tcctctatcc cctgccggcg aaaagcgcgg gcgtgtgggg cgcgcacgcc gcggcggcct
     2761 tcgtcggcgg cctggtgttc atcggcgttg gcttcctgct gttctacctc gattgcgcgg
     2821 tggcgatcct gcgccgctac ggcagcctcg cgaaggcgct tgggctcgac cagctgttcg
     2881 gctcgggccc ggtggacacg tcgctcccgc ccacggtcgt ggcgagcacg atggtcgtca
     2941 tcatcaacat cctcggcatc ctcgccggcg ccgtggtgct ggtgatgacc ctcgtcaata
     3001 tctacgtgcc ggccatcgcg ttcgacgcgc tgctcatgaa gaacctgatc ttcttcttcg
     3061 gccacgtctt catcaacgcc accatctaca tggcggtaat cggggtctac gagatcgtgc
     3121 cgcgctacac cgggcgcccc tggacgatca gccggccatt cctcgcctcg tgggcggcgg
     3181 tgacgctgat cgtgatggcg gtgtacccgc accacctgct gatggactac gtgatgccga
     3241 agtgggcggt cgtgaccggc caggtcctgt cgtacctgag cggtatcccg gtcctgctgg
     3301 tgaccgtcta cggcgcgctg gtcaacatcc accgctcggg gctgaagtgg gatctgccgc
     3361 tgcgcctcat cgtcttctcg ctgttcggct gggcggcggg catcatcccg gcgatcatcg
     3421 acggcgtgat ccgcgtgaac ctggtcatgc acaacaccca gtgggtgccc ggccacttcc
     3481 atttctacct gctgctgggc ctggtgccga tgctgctcgg gacgatcctc tacgcctgca
     3541 cgcgcgagcg ctgggccgag accgcgctcg accaggcggc gttctggacc tacggcgtcg
     3601 cgggcgtggt gttctgtttc gtgttcctcg cgggcggctg gcagggcgtg ccgcggcgct
     3661 tcgcggttca cgacgcggcc tggctcggct tcgcccagat cggcacggtg gcagcgaccc
     3721 tggtgctcgt cgccgcggtg gtgctcggcg tgcggctgct cgcgcgcctg ccgcgcgcgt
     3781 cactctccgc atgagcgcgc agcgcacctc gattgcggcg gccctcgtgc tggcggcggg
     3841 gcttgccgtc acgcatgcgg cgaccgacgg cttcgaggct tttacgctcg agtcggcgcg
     3901 gcggctcgcc gcgttgcgct cgccggtgcg cgttcccgat ctcgccctgg aactcgccga
     3961 cggcggccgc gcgcgcctct ccgcgatgcc gggcgaggtg ctgctggtcg atttcgtcta
     4021 tacgaactgc ccgacggtgt gcgcggcgct cggctcggtg tacgcgcgcc tgcagcagcg
     4081 cctcgcgccg gagatcgccg cgggcggggt acgcctgctg tcggtgacgt tcgaccccgc
     4141 gcgcgatggc cccggggagc tgcgcgcctc ccgcgcgcgt ttcagc
//
LOCUS       DBNGSG010000381         2246 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1482353, whole genome shotgun sequence.
ACCESSION   DBNGSG010000381 DBNGSG010000000
VERSION     DBNGSG010000381.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1482353"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /gene="truD"
                     /locus_tag="AC158Q_09860"
     CDS             <1..389
                     /gene="truD"
                     /locus_tag="AC158Q_09860"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: protein motif:HMM:NF013322.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HIR3340123.1"
                     /translation="RMLAGDWAMKMDNGACFAVEDATREQPRADRFEISPTGILFGSR
                     VSWATGEPGEIERAVVVEQGSTPEGLVDAAKTCGFRGERRSLRVPLADLEWTLDEGVL
                     TLAFSLPPGAYATSVLRELMKTPSLG"
     gene            438..1685
                     /locus_tag="AC158Q_09865"
     CDS             438..1685
                     /locus_tag="AC158Q_09865"
                     /EC_number="4.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008473 - ornithine cyclodeaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase, nickel-pincer
                     nucleotide-dependent"
                     /protein_id="HIR3340124.1"
                     /translation="MAAAAQETVVLSGHIIDSLILAKVLDTILMMGGTFDLEEVSIGK
                     TRNESSRARIVVRAGSAKLLSDILRAIQPHGASVESESDCHYRAAPADGVFPEDFYAT
                     THLPTQIRLAGQWIDVEGIEMDLGIVVDKKTSRARAVPMAGVTRGDAVVIGRSGIRVT
                     PLQRPQEHDVFSFMESQVSAERPHGHIIADVARRMQLLREGHRAGKPGCKVLVAGGPA
                     IIHAGGRDALTWLIDEGFVHVLFCGNALAAHDMEADIYGTSLGYGLSAGRVVPHGHEH
                     HLRTINRVRGIGSIEKAVDTGVIKRGIMASCVRRGVQVVMAGTIRDDGPLPGVITDSV
                     EAQAAMRAAIPGVGLALLVASTLHAVATGNLLPATIPTVCVDVNPAVPTKLADRGSFQ
                     AVGLVMDAASFLRDVARELGWKG"
     gene            1673..>2246
                     /locus_tag="AC158Q_09870"
     CDS             1673..>2246
                     /locus_tag="AC158Q_09870"
                     /EC_number="3.5.3.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016403 - dimethylargininase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylarginine dimethylaminohydrolase family
                     protein"
                     /protein_id="HIR3340125.1"
                     /translation="MERMSRLLVCPPDYFRIDYEINPWMRRSNVVEPESAVRQWHRLM
                     EVLERDVGAVLERMKPVPGLPDLVFTANAGIVVGRKAVPSRFRYPERQREEAHFEGWF
                     RDHGYEIVLLEPDLYFEGAGDLLGFPDGWFGGYRQRSDIRAFPILSEMFEREIRPVEL
                     VDSRFYHLDTCFCPLSGGELLYFPPAFDRYA"
BASE COUNT          409 a          651 c          733 g          453 t
ORIGIN      
        1 atcgcatgct tgccggcgat tgggccatga aaatggacaa tggtgcctgc tttgcggttg
       61 aagacgccac ccgtgaacag ccacgcgccg accggttcga aattagtcct acagggattc
      121 ttttcggttc acgggtttcg tgggctaccg gcgagccggg tgagatcgag agggctgtcg
      181 tcgtcgaaca ggggtctacc cctgagggcc tcgtcgatgc ggcaaaaaca tgcggctttc
      241 gcggggaacg ccgctcgctc cgggtgccac tggccgacct ggaatggacg ctcgatgaag
      301 gcgtactcac gctggcattc agcctgcctc cgggcgccta tgccacgagt gtcctgcgtg
      361 agctgatgaa aaccccctcc ctggggtaac cgcggcgcgc cccctgcgtg agaaaatcaa
      421 cacaagacag gagacgcatg gccgctgccg cccaagaaac cgttgtgctg agcgggcaca
      481 tcatcgattc gctgatcctg gcgaaggtgc tggataccat cttgatgatg ggcggaacct
      541 tcgatctcga ggaggtctcg atcgggaaga cgcgcaacga gtcttcccgt gcgcgcattg
      601 tcgttcgcgc cggttccgcg aagctgctct cggacattct ccgggccatt caaccgcacg
      661 gcgcgtcggt ggaaagcgaa tcggactgtc actatcgtgc ggcgcctgcc gacggcgtat
      721 tccccgaaga tttttacgcg accactcact tgcctactca gatcaggctt gcgggacaat
      781 ggatcgacgt ggaaggcatt gagatggatt tgggcatcgt cgtcgacaag aagacctccc
      841 gggcacgcgc cgtgccgatg gccggtgtca cccgcggcga tgcggtggtg atcggacgca
      901 gcggcatccg ggtgactcct ctccaacggc ctcaagagca cgatgtcttc agtttcatgg
      961 agtcacaggt atccgccgag cggcctcacg gccatattat cgccgatgtg gcgagacgca
     1021 tgcagttgct acgcgagggg caccgggcag gcaagcccgg ctgcaaggtg ctggtggccg
     1081 gagggccggc aatcatccac gcaggaggac gcgatgcatt gacatggctc atcgacgagg
     1141 ggttcgtcca tgtcttgttc tgcgggaatg cgctggcggc gcacgatatg gaggcggaca
     1201 tctatggtac ctcattgggg tacgggctgt cggccggcag agtcgtgccc catggccatg
     1261 agcatcattt gcgcacgatc aatcgcgtgc gcgggatcgg tagcatcgaa aaagcggtcg
     1321 acaccggtgt gatcaagcgc ggcatcatgg catcctgcgt caggcgaggc gtgcaggtcg
     1381 tgatggcagg gacgatccga gacgatggcc cgttacccgg cgtcatcacc gattcggttg
     1441 aggcgcaggc ggcgatgcgt gcggccattc cgggagtcgg cctggcgttg cttgtggcct
     1501 cgacccttca tgccgtcgcc accggcaact tgctgcctgc gacgattcca accgtgtgcg
     1561 tcgacgtgaa tcccgctgtg ccgaccaagc tggctgaccg cggcagtttt caagcggtag
     1621 gactcgtcat ggatgctgca tccttcttgc gcgatgtggc gagagaattg ggatggaaag
     1681 gatgagccgg ttactggtgt gcccccccga ctatttccgc atcgactatg aaatcaatcc
     1741 atggatgcgc cgatccaacg tcgtggaacc cgaaagtgcg gtccgccagt ggcatcgctt
     1801 gatggaggtg ctcgagcgcg acgtaggcgc cgtgctagag cggatgaagc ctgttcccgg
     1861 acttccggac ctggtgttca ccgccaatgc gggaatcgtg gtgggccgca aggccgttcc
     1921 cagccgtttc cgctaccctg agcgacagcg cgaggaagcg catttcgaag gctggtttcg
     1981 ggaccacggc tacgagatcg tattgcttga gcccgacctg tatttcgagg gcgccggcga
     2041 cctgctgggg tttccagacg gctggtttgg cggatacagg caacgctcgg acatccgtgc
     2101 gttcccgatt ctcagcgaga tgtttgagcg ggaaatccgt cccgtggagt tggtcgacag
     2161 ccgcttctac catctcgata cctgtttttg tccgctcagc ggaggagaac tgctgtattt
     2221 tcctccggcg tttgaccggt atgcgc
//
LOCUS       DBNGSG010000382         4789 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3690477, whole genome shotgun sequence.
ACCESSION   DBNGSG010000382 DBNGSG010000000
VERSION     DBNGSG010000382.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4789)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4789)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4789
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3690477"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            307..1248
                     /locus_tag="AC158Q_09875"
     CDS             307..1248
                     /locus_tag="AC158Q_09875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3340126.1"
                     /translation="MAHISVVTPVYRAEACLEELYRRLVGALSAITQDFEIVMVEDCG
                     GDRSWEIIQALARRDARVKGIQFSRNFGQHYGITAGLDHCDGDWVVVMDCDLQDRPEE
                     IPRLYAKAREGYEIVIAHRGRRSDPLAKRMTSWVFYRLFSWLAEIDYDGQAGNFRIVS
                     RKVVGSFRQMREQLRFFGGLINWMGFPAATIDVQHDERYAGPSTYTLAKLWKLAFETI
                     VAYSDKPLRLSVRFGFVLAFLAFCYGLYIVVHALLYGSAISGWASLIVSMYFIGGIII
                     AILGVIGIYLGKTFDEAKKRPLYLVMNTTFHGERRAD"
     gene            1226..1897
                     /locus_tag="AC158Q_09880"
     CDS             1226..1897
                     /locus_tag="AC158Q_09880"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3340127.1"
                     /translation="MASGELIRRTPWDRAALACDTFELAAASREAMAQARAPGHYTVK
                     VDPLCDKRILHENGFYYCDTLLEPYCTRERLAGATHPDATLTRETPLPDVLRIGHGAF
                     AHGRFHRDFHVDRAAADRRYDGWLRQLHGAGQVVGLLHRGALAGFIATEGGKLVLHAV
                     AEPHRGRGLAKFWWTALCRELLAAGHGEVTSSISAANLAALNLYASLGFRLRNPVDVY
                     HRVIR"
     gene            1894..2256
                     /locus_tag="AC158Q_09885"
     CDS             1894..2256
                     /locus_tag="AC158Q_09885"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR3340128.1"
                     /translation="MSYFYVACTVLLTVYGQLVIKWQVLAAGPLPDATGDKFLFLARL
                     LMNPWIVSALAAALAAAMAWMAAMTRLDLSHAYPFLSTVFVLVPLLSVVLFNEPVTSP
                     KVIGLALVVAGIVIGGQG"
     gene            2253..3131
                     /locus_tag="AC158Q_09890"
     CDS             2253..3131
                     /locus_tag="AC158Q_09890"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008642720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3340129.1"
                     /translation="MRLCLRCRADIAGGGWRCPRCGFEPPGIGGFPAFAPELAQGASG
                     YDPAHFAELARLEAGNFWFRARNRLLAWAMRRYFPGARSFLEIGCGTGFVLAGIAAAF
                     PALKLSASEAAAEGLAHAAARVPGASLMQMDARHIPFRDEFDVVGAFDVIEHVEDDRA
                     VLAALRAAAAPAGGLLLTVPQHPNLWSEFDVRAGHVRRYRAAELRGKVLEAGFEIVKM
                     TSFVTLLLPLMLASRLTQRRPRRDYDPLAELRIAPWLNAVLEKTLDAERLLIRAGVPL
                     PAGGSLLVVARRRERP"
     gene            3192..4328
                     /gene="rffA"
                     /locus_tag="AC158Q_09895"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             3192..4328
                     /gene="rffA"
                     /locus_tag="AC158Q_09895"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose:2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HIR3340130.1"
                     /translation="MKIPFNKPFTIGAELENIAAAVREGHLAGDGRFTRQCHQWLEQN
                     LGARRALLTHSCTAALEMAAILCDLKPGDEVIMPSFTFVSTANAFVLRGAVPVFVDIR
                     ADTLNLDETLAAAAVTPRTRAIVPVHYAGIACEMDAIMALAQARGLLVVEDAAQGMLA
                     DYKGKKLGAIGHLGCLSFHETKNVISGEGGALLVNDERLVARAEIIREKGTNRAQFFR
                     GEVDKYTWVDIGSSYLPSELIGAFLWAQLEHAGEINARRMRLCAAYREELATLEAAGL
                     LRLPQAPPAGVTGNGHMFYVLLPDSAARARLIAHLKSLGIHSVFHYVPLHSSPAGRRA
                     GRAAGPMTVTDDASGRLLRLPLYYGLDEADARRIAAAVRAFFGK"
     gene            4334..>4789
                     /locus_tag="AC158Q_09900"
     CDS             4334..>4789
                     /locus_tag="AC158Q_09900"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3340131.1"
                     /translation="MAPAQPSHHDGGLHRVLNRAGIYGALQKLLLRSGSRERYVREYV
                     RPTPGCRILDIGCGPADILEYLPQSVGAYAGFDMNPGYIEAAQRRWGGRGSFRCEKVE
                     AAALPAGGAYDIVLANGIVHHLDDAEAARLFEVAHLALRPGGRLVTYDTV"
BASE COUNT          671 a         1703 c         1628 g          787 t
ORIGIN      
        1 ctcgaccgcg gtcgcatcgc cgagaccggc acccaccgcg agctgctcgc ccggggcggc
       61 atctacgaca agctgtaccg catccagttc gccagtgcgg agccggcgaa cggcgggcgc
      121 taggcacgga aaacggaaca tgacacccgt cagcggcctc agtaattgtg ctttcaactc
      181 gcacgcctcc tgtccggcca ctttgcgcga cgccgggggt cagatcccca gcggggctgg
      241 ggcccctctt ccgcccctcg acgcgccccg acactcgtca cccgtcactc gcgccgggcg
      301 atagcgatgg cgcatatctc cgtcgtcacc cccgtctacc gggcggaagc ctgcctcgag
      361 gagctctacc gccggctcgt cggcgcgctc tccgccatca cgcaggactt cgagatcgtc
      421 atggtcgagg actgcggcgg cgaccgctcg tgggaaatca tccaggcgct ggcgcgcagg
      481 gacgcgcggg tgaagggcat ccagttcagc cgcaacttcg gccagcatta cggcatcacc
      541 gccggtctcg accattgcga cggcgactgg gtggtggtga tggactgcga cctgcaggac
      601 cggcccgagg aaatcccgcg gctctacgcc aaggcgcgcg aaggctacga gatcgtgatc
      661 gcgcaccgcg gcaggcgcag cgacccgctc gccaagcgga tgacgtcgtg ggtgttctac
      721 cgcctcttca gctggctcgc ggagatcgac tacgacggcc aggcgggcaa cttccgcatc
      781 gtttcgcgca aggtggtcgg cagcttccgc cagatgcggg agcagctgcg cttcttcggc
      841 gggctcatca actggatggg gtttcccgcc gcgacgatcg acgtgcagca cgacgagcgc
      901 tacgccggcc cctcgaccta caccctcgcc aagctgtgga agctcgcttt cgagaccatc
      961 gtcgcctatt ccgacaagcc gctgcgcctg tcggtgcgct tcggcttcgt gctcgccttc
     1021 ctcgccttct gctacggcct ctacatcgtc gtgcacgcgc tgctctacgg ctccgcgatc
     1081 agcggctggg ccagcctcat cgtttccatg tatttcatcg gcggcatcat catcgccatc
     1141 ctcggcgtga tcggcatcta cctcggcaag accttcgacg aggcgaagaa gcggccgctc
     1201 tacctcgtga tgaacacgac attccatggc gagcggcgag ctgattaggc gcacgccctg
     1261 ggaccgcgcg gcgctcgcct gcgatacctt cgagcttgcc gctgccagcc gggaagccat
     1321 ggcgcaggcg cgcgcccccg gccactacac ggtcaaggtc gaccccctct gcgacaagcg
     1381 catcctgcac gagaacgggt tctattactg cgatacgctg ctcgagccgt actgcacccg
     1441 ggagcgcctc gccggcgcca cccaccccga cgcgacgctg acgcgcgaga cgccgctgcc
     1501 cgatgtgctg cgcatcggcc atggcgcatt cgcgcacggc cgcttccatc gcgacttcca
     1561 cgtcgatcgc gcagcggccg accgccgcta cgacggctgg ctgcggcagc tccacggcgc
     1621 gggccaggtg gtcgggcttc tccaccgcgg cgcgctggcg ggcttcatcg ccacggaagg
     1681 cggcaagctg gtgctgcacg cggtggcgga gccgcatcgc gggcgcgggc tcgcgaaatt
     1741 ctggtggacc gcgctgtgcc gggaactcct cgccgccggc cacggcgagg tgacgagctc
     1801 gatctcggcc gccaacctcg ccgcgctcaa tctgtacgcc tccctgggct tccgcttgcg
     1861 caatcccgtc gatgtctacc accgggtgat ccgatgagct atttctacgt tgcctgcacc
     1921 gtgctcctca ccgtctacgg ccagctcgtg atcaagtggc aggtgctcgc ggcgggaccg
     1981 ctgcccgacg ccaccggcga caagttcctg tttcttgcgc ggctgctgat gaacccctgg
     2041 atcgtctccg cgctcgccgc ggcgctcgcc gccgcgatgg cgtggatggc cgcgatgacc
     2101 cggctcgatc tctctcacgc ctatcccttc ctcagcaccg tcttcgtgct ggtgcccctg
     2161 ctgtccgtgg tgctgttcaa cgagccggtg acctcgccca aggtgatcgg gctcgccctg
     2221 gtcgtggccg gcatcgtgat cgggggccag ggttgaggct gtgcctgcgc tgccgcgcgg
     2281 atatcgcggg cggcggctgg cgttgcccgc ggtgcggttt tgagcctccg ggcatcggcg
     2341 gctttccggc cttcgcgccc gagctcgcgc agggcgcgag cggctacgac ccggcgcact
     2401 tcgccgagct ggcccggctc gaggcgggca atttctggtt ccgggcgcgc aaccggctgc
     2461 tggcatgggc gatgcggcgc tattttcccg gggcgcgcag ctttctcgaa atcggctgcg
     2521 gcaccggttt cgtgctggcg ggaatcgcgg cggcgtttcc ggcactgaag ctctcggcga
     2581 gcgaagcggc ggccgaaggg ctggcgcatg cggcggcgcg ggtgccgggg gcgagcctga
     2641 tgcagatgga cgcgcgccac attccgttcc gcgacgagtt cgacgtggtc ggtgcgtttg
     2701 acgtcatcga gcacgtcgag gacgaccgcg cggtgctggc cgcactgcgt gcggccgccg
     2761 cgcccgcggg cggcctgctg ctcacggtgc cgcagcaccc gaacctttgg agcgaattcg
     2821 acgttcgcgc cggccacgtg cgccgctacc gcgcggccga gctgcgcggc aaagtgctgg
     2881 aagcggggtt cgagatcgtt aaaatgactt cctttgttac cctgctgctg ccgctcatgc
     2941 tcgcttcccg attgacgcaa cgccgcccgc gccgtgatta cgacccgctt gcggagctga
     3001 ggatcgcgcc gtggctcaac gcggtgctgg agaagacact ggacgcggaa cgcctgctga
     3061 tccgcgcggg cgtcccgctg cccgccggcg gctcgctgct ggtcgttgct cgccggcgcg
     3121 agcggccgtg acgggtgacg ggtgacggcc agctcacttt cgactttcga ctttcgactt
     3181 ccgccttcgg aatgaagatc cccttcaaca agccgttcac catcggggcc gagctggaga
     3241 acatcgcggc cgcggtgcgc gagggccatc tcgccggcga cggccgcttc acgcgccagt
     3301 gccaccagtg gctcgagcag aacctcggcg cccggcgcgc gctgctcacg cattcctgca
     3361 ccgccgcgct cgagatggcg gcgatcctgt gcgacctgaa gcccggcgac gaggtgatca
     3421 tgccgtcttt caccttcgtt tccaccgcca atgccttcgt gctgcgcggt gccgtgccgg
     3481 tgttcgtgga catccgtgcc gacacgctca acctggacga aacgctcgcc gccgcggcgg
     3541 tgacgccgcg gacccgcgcc atcgtgccgg tgcactacgc cggcattgcc tgcgagatgg
     3601 acgcgatcat ggcgcttgcg caggcgcgcg gcctgctggt ggtcgaagat gccgcgcagg
     3661 ggatgctggc ggactacaag gggaagaagc tcggcgccat cggccacctg ggctgcctgt
     3721 ccttccacga gaccaagaac gtcatcagcg gcgagggcgg ggcgctgctc gtcaacgacg
     3781 agcgcctggt ggcgcgggcg gagatcatcc gcgagaaggg caccaaccgc gcccagttct
     3841 tccgcggcga ggtggacaag tacacctggg tggacatcgg ctcgtcgtac ctgccgagcg
     3901 agctgatcgg ggcgttcctg tgggcccagc tcgagcatgc cggcgagatc aacgcccggc
     3961 gcatgcggct gtgcgcggcc taccgcgagg agctcgcgac gctcgaagcc gccggcctgc
     4021 tgcggctgcc gcaggcgccg cctgcgggcg tcaccggcaa cgggcacatg ttctatgtcc
     4081 tgctgccgga ttccgcggcg cgggcgcggc tgatcgcgca cctgaagtcg ctcggcatac
     4141 actccgtgtt tcattacgtg ccgctgcatt cctccccggc ggggcgcagg gccgggcgcg
     4201 ccgccggccc gatgaccgtg accgacgacg cgtcgggccg gctgctgcgg ctgccgctct
     4261 attacgggct cgacgaagcc gacgcccggc gcatcgccgc ggcggtgcgc gcgttcttcg
     4321 gaaaatagcg cgcatggcgc cggcgcaacc cagccaccac gacggcggcc tgcaccgcgt
     4381 gctcaaccgg gcgggcatct acggcgcgct gcagaagctc ctgctgcgca gcggcagccg
     4441 ggaacgctac gtgcgcgagt acgtgcggcc gactccgggc tgccgcatcc tcgatatcgg
     4501 ctgcggtccg gccgacatcc tcgagtacct gccgcagtcc gtcggcgcgt acgcgggctt
     4561 cgacatgaac ccgggctaca tcgaggcggc gcaacggcgg tggggcggcc gcggcagctt
     4621 ccgctgcgag aaggtggagg cggcggccct gcccgcgggc ggggcgtacg acatcgtgct
     4681 tgccaacggc atcgtgcacc acctggacga cgcggaagcc gcccggctgt tcgaggtcgc
     4741 gcatctcgcg ctgcggccgg gcgggcggct cgtcacctac gacaccgtc
//
LOCUS       DBNGSG010000383         3316 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1993246, whole genome shotgun sequence.
ACCESSION   DBNGSG010000383 DBNGSG010000000
VERSION     DBNGSG010000383.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1993246"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1814
                     /gene="selB"
                     /locus_tag="AC158Q_09905"
     CDS             <1..1814
                     /gene="selB"
                     /locus_tag="AC158Q_09905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HIR3340132.1"
                     /translation="FIRNMLAGVCGIDFTLLVVAADDGVMPQTVEHLNILELLGISRG
                     IAVITKIDRVAPERADAVRAEVTALLAPTRLAGIPVIAVSTVTGAGIDELKQQLAAAA
                     SAFAARAAEGQHFRFAIDRAFTIAGSGTVVTGTVFNGAVAVGDRLVISPKGTPVRVRG
                     IQMRGQPAERAQAGQRCALNLTGADLETVARGDWVLHEAIHAPTQRIDARVTVLGAEA
                     KALKHWTSLHVHLATADIPARISLPGENSIAPGASAIAQLVLAKPAGALNGDRFILRD
                     QSARRTLGGGVVVDPYARAARRASGARLAELAALEQGSPEATLDATLSDAGRGVDISR
                     FERVFNLKPERAAALYAAAGVVVLGKEHRTGVTRSLRDSLRERTLAALRDFHRAQPQA
                     VGLEAAALRKQLAPQLHAEAFQALVRELADERKLEIASNVVRLPGHDATSNPEDQRLW
                     QLVLPALQAAGFAPPPVADLAAALKLKEAALKDFLHRKSRSGEVMRVPPDRFYPRATL
                     ATLAATAHALARANPGGFTAAQYRDATGVGRSLAIEILEFLDTLAITQRVGDARKARK
                     DFAPLLGAAEPAPPPAKTAAAKSASQVGKPRPSPYRR"
     gene            2012..2914
                     /locus_tag="AC158Q_09910"
     CDS             2012..2914
                     /locus_tag="AC158Q_09910"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3340133.1"
                     /translation="MNYDLIVIGEGVSGLACAGHATRAGLKVATLESTLFGGLVLNVN
                     ELDGYPGAESGTDLASALMQANADAGVTSIQEEVTAIRPAGGAFEIATGAGAYAARQV
                     VIASGARLKKLGVPGEKEYEGRGVSWCADCDAPMFQGEDVVVVGGGDSAMQEALVLAN
                     FCRSVRVAHRGEKLRARKHLAEKVTANPKISIMWKTTVDAILGGKMVEKVRLREAGGK
                     TQEIACAGVFAYIGLEPNAAFAPPAIGRDGRGHLVTNDEFETAMKGIWAIGAVRAGYS
                     GLLRDAVGEAERVAKAVHVRLATD"
     gene            3109..3203
                     /locus_tag="AC158Q_09915"
     tRNA            3109..3203
                     /locus_tag="AC158Q_09915"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3143..3145,aa:Sec,seq:tca)
BASE COUNT          574 a         1138 c         1084 g          520 t
ORIGIN      
        1 gcttcatccg caacatgctg gccggcgtgt gcggcatcga cttcaccctg ctggtggtcg
       61 ccgccgacga cggcgtgatg ccccagaccg tcgagcacct gaacatactc gagctcctcg
      121 gcatctcgcg cggcatcgcc gtgattacca aaatagaccg cgttgcgccc gagcgcgcgg
      181 atgccgtgcg cgcggaagta accgccctgc tcgcgccgac ccggcttgcc gggatacccg
      241 tgatcgccgt ctcaaccgtc accggggccg ggatcgacga gctcaagcag caactcgccg
      301 cggccgccag cgcgttcgcc gcgcgggcgg cggaaggcca gcatttccgg ttcgcgatcg
      361 accgcgcctt caccatcgcg ggcagcggca ccgtggtgac gggcaccgtc ttcaacggcg
      421 ccgtcgccgt tggcgaccgc ctggtgatct cgcccaaggg cacgccggtg cgcgtgcgcg
      481 gcatccagat gcgcgggcag ccggcggaac gcgcgcaggc cggtcagcgc tgcgcgctga
      541 acctcaccgg cgccgacctg gaaaccgtcg cgcgcggcga ctgggtgctg cacgaagcca
      601 tccacgcgcc gacgcagcgc atcgatgccc gcgtcaccgt tctcggcgcc gaggcgaaag
      661 cgctcaagca ctggacgtcg ttgcacgtgc acctcgccac cgccgacatc cccgcgcgca
      721 tctcgttacc cggggagaat tccattgcgc ccggcgcctc cgcgatcgcg caattggtgc
      781 tggccaagcc ggcgggcgcg ctcaacggcg accgcttcat cctgcgcgac cagtccgcgc
      841 gccgcaccct gggcggcggc gtggtggtgg acccttacgc gcgcgcggcg cggcgcgcat
      901 ccggcgcccg gctcgcggaa ctcgcggcgc tcgagcaggg ctcgcccgag gcgacgctcg
      961 atgccacgct gagcgacgcc ggtcgcggcg tcgatatttc ccgattcgag cgagtattca
     1021 acctcaagcc ggaacgcgcg gcggcgctct acgccgccgc gggcgttgtc gtgctcggca
     1081 aggagcatcg caccggcgtc acgcggagcc tccgcgacag cctgcgcgag cggacgcttg
     1141 ccgcattgcg cgactttcac cgcgcccagc cgcaggcggt gggactggaa gccgcggcgc
     1201 tgcgcaagca gctcgcgccg caactgcatg cggaggcgtt ccaggcgctc gtgcgcgaac
     1261 tcgcggacga acgcaagctg gaaatcgcga gcaacgtcgt gcgcctgccg gggcacgacg
     1321 cgacctccaa tccggaagac cagcggctgt ggcagctcgt gctccccgca ttgcaggcgg
     1381 caggcttcgc cccgcccccg gtggcggatc tggcggcagc gctgaagctc aaggaagcag
     1441 ccttgaagga cttcctccac cgcaagagcc gcagcggcga ggtcatgcgc gtgccgccgg
     1501 accgcttcta tccgcgcgcc acgctcgcca cgctcgccgc gaccgcccac gcgctcgccc
     1561 gcgcgaaccc cggcgggttc accgccgccc agtaccgcga cgccaccggc gtcggcagaa
     1621 gcctcgcgat cgagatcctg gaatttctcg acacgctcgc tatcacccag cgcgtcggcg
     1681 acgcacgcaa ggcgcgcaag gatttcgcgc ccctcctcgg cgcggccgaa ccggcgccac
     1741 cgccggcaaa aacggctgcc gcaaagtccg cgtcccaggt cggcaagccg cggccgtcgc
     1801 cttaccggcg ctgagggaca ggattaacag agctcgatct aaaaaccgca cacccgctaa
     1861 aaacgtaatt ggacaggatt aacaagattt acaggattca atcaaaatac ttccagactt
     1921 tattgagttt taatcctgtt aatcctgtct attaatgggt ttgacgggtt tttgaatagt
     1981 tgattctgtc tatttcgaac ccgatttcac aatgaactac gatctgatcg tcatcggcga
     2041 gggcgtctcc ggcctcgcct gcgcaggcca cgcaacccgc gccgggctga aggtggcgac
     2101 gctcgagtcc accctgttcg gcgggctggt gctgaacgtg aacgagctcg acggctatcc
     2161 cggcgccgaa tcgggcaccg atctcgcctc cgcgctcatg caggcgaacg ccgacgcggg
     2221 cgtgaccagc atccaggaag aagtcaccgc catccgcccc gccggcggcg cgttcgaaat
     2281 cgcgaccggg gccggcgcct acgcagcgcg ccaggtggtc atcgcctccg gcgcgcggct
     2341 gaagaagctg ggcgtgcccg gcgagaagga atacgagggg cgcggcgtct cgtggtgcgc
     2401 cgactgcgac gccccgatgt tccagggcga ggacgtggtc gtggtcggcg ggggcgactc
     2461 cgcgatgcag gaagcgctgg tgctggcgaa tttctgccgc agcgtgcggg tagcgcatcg
     2521 cggcgagaag ctgcgcgcgc ggaagcacct cgccgaaaaa gtcacggcca atccgaagat
     2581 ctccatcatg tggaaaacca cggtggacgc gatcctcggc ggtaaaatgg tcgagaaggt
     2641 ccgcttgcgg gaagcgggcg gaaaaacgca ggaaatcgcc tgcgccggcg tgttcgccta
     2701 catcgggctc gagcccaacg cggccttcgc gccgcccgcg atcggccgcg acgggcgcgg
     2761 ccatctcgtc acgaacgatg agttcgagac cgcgatgaaa ggcatctggg ccatcggcgc
     2821 ggtgcgcgcc ggctacagcg ggctcctgcg cgacgcggtc ggcgaggcgg aacgtgtggc
     2881 aaaagccgtt cacgtccgtc ttgcgacgga ctgatgccag atgtaatgca gcaataaaat
     2941 agccgccaag acgccaagaa cgccaaggaa cccggggcaa cgaaaaccaa cattgctaca
     3001 cgctgatggc aaatcgattt tcgttcttat ctcgcggtgt ttcggttatg gtcttgcctt
     3061 gcttggcggc cttggcgcct tggcggctaa tttttggttt tggaaaacgg aagggaatcg
     3121 cccccggtgg ggcggccgga cttcaaaccc ggttagggcc gcttgcggtt ctgtgtgggt
     3181 tcgactccca ctctcttccg ccaatttttt cgccgtcatc cccgcgaagg cggggatcca
     3241 gaaaatagtg ggtcgcctag actcccgcct tcgcgggagt gacgggccat actgctatcc
     3301 ctgatccgca atttca
//
LOCUS       DBNGSG010000384         4353 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2502497, whole genome shotgun sequence.
ACCESSION   DBNGSG010000384 DBNGSG010000000
VERSION     DBNGSG010000384.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4353)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4353)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4353
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2502497"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            594..1565
                     /locus_tag="AC158Q_09920"
     CDS             594..1565
                     /locus_tag="AC158Q_09920"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3340134.1"
                     /translation="MRRYIASALVALTSIVAAGAVCAQAYPTRPVRVIIPFPPGGNVD
                     VFGRVLWPQVEKELGQTVVIDNRGGANGILGADIVANATPDGYTFLHTSFAFAVNPSI
                     VIKMPFDVTRDFVPVTDVALGTGYLMVAHSKFAPRTVKELIDLAKQQPGKVRYSTAGV
                     GNGQHLAGALFAEKAGIDLLHVPYKGGGPAMTAALGGETQLHFPAPAVGIPHVKSGRV
                     RGLGFTGSKRLISLPDVPTIGETVKGYVADAGWHGVFAPAKTPPAIVKKVHESIAKAL
                     KVPHVRDHFVNAGYEPQGNTPAEWNKRFRADLKRYAEITRIAKIERR"
     gene            complement(1622..2545)
                     /locus_tag="AC158Q_09925"
     CDS             complement(1622..2545)
                     /locus_tag="AC158Q_09925"
                     /inference="COORDINATES: protein motif:HMM:NF015445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR3340135.1"
                     /translation="MTAPIVLKFGGYQEPASIHNRAAGRFGGLLTKQLGERIRFELIG
                     SVLKLGRGSGDLVPMVESGELSFCYMSTVRFSKVAPELQILELPFVVKNRATVFEALD
                     GEFGQAAARKIDANTPCRFLGFWDNGFRYLSNRVRPIRKPEDCRGIRIRTQMSDLHGE
                     VFRALGFEPIKSDIKEFVEQVGGDRFQAQDNPLTNIYNFGVHTHHRYITLTAHFWGAS
                     ALVCNAAQYQGWPRDVQAAVDAAAKEATAYQRQLAAVEDAEIMKKLDPAMNEVIELTD
                     TERAVFKRALQPVVEKYRKQLGAGLFDRLGA"
     gene            complement(2542..3423)
                     /locus_tag="AC158Q_09930"
     CDS             complement(2542..3423)
                     /locus_tag="AC158Q_09930"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019474.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3340136.1"
                     /translation="MTTATLTRYDIDVEDVEYIRHGNKPLLARLYKPRGAGPFPLIID
                     LHGGAWCNKDRTSDAGTDEPLARSGVTVVALDFRMPPDAGYPASLQDVNYAIRWCKAR
                     AGELKTRPDLVGILGVSSGGHQAMLTALRPNDPRYAALSLPGKTADAAINATVRCAVL
                     CWPVIDPLGRYQYAKSSQNGPLKKQAGNWLQCHDQYWQGGEAAMAEGNPTLLLERGEK
                     VPLPPVIYLQGTADVAHPVPNRERFIANYRKAGGRVELHLFEGMGEAFITNDPASPAA
                     RSAIEKIIEFVHREMPA"
     gene            complement(3544..>4353)
                     /locus_tag="AC158Q_09935"
     CDS             complement(3544..>4353)
                     /locus_tag="AC158Q_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase C-terminal
                     non-catalytic domain-containing protein"
                     /protein_id="HIR3340137.1"
                     /translation="DYGRLLQKRFFDYFLKGENNGWDRQPRVQLQVRHTDRFVERHES
                     EWPLARTQWTRFHLDPGNHSLTREPAGSAGSISYDGLGDGVTFLSPPFERETEITGPL
                     AAKLFVSSSTADADLFLVFRVYTPDLREVVFMGAIDPHTPVAQGWLRASHRQLDPGLS
                     TEYRPYHTHDRKQPLSPGEIVQLDIEIWPTSIVVPAGYRIALSVRGRDYEWQKATGAR
                     LSNFKNELLGCGPFLHDDPRDRPASVFGGKVTLHVGPRHPSYILLPVIPKA"
BASE COUNT          758 a         1360 c         1430 g          805 t
ORIGIN      
        1 taggcctcgc ggttggcttc ggccaggtcg taccaggaaa tccagagcgc tcggtccacg
       61 atgtgtcccc cttgctagag cctatctcaa taataggcac gtgtgcgacg gaggcgatgt
      121 gggatgcagt tctaggagga tcgcgacgac gcatagactc tatgttagga gcgaccgaca
      181 acgaaatgcg cccagatcgc cccgtccctc cgggttgcgg ccaggacggg cgtctgcttc
      241 gttgcccggc ttgaccctat cgacataggg cctgcaccgt gcgcctcaca tccatcccgt
      301 cctggccaca acgcatcacg cgcctattat tgagataggc tcctaccaaa tccattctga
      361 aagtccggcc ggccgcctgc gtccgcgggc gtgcgtcctg aattgagcgg catgatggaa
      421 tggccggaca gttttccggt gatcgctcga aacagccccg gaactcgatc ggaaatcccc
      481 caagggttgg ttggaagggt attcggggaa cattcggttt gcagtctctc cagattcggc
      541 taccatcgaa tcgcttcagc ttgataatat tcaatccacg aggaggaagc attatgcgtc
      601 gctacatcgc ctcagcactg gttgccctga catccatcgt cgccgcgggc gcggtctgcg
      661 cccaggctta ccccacccgg ccggtgcgcg tcatcattcc attcccgccg ggcggcaacg
      721 tcgacgtctt cgggcgggtg ctgtggccgc aggtggagaa ggaactgggg caaacggtcg
      781 tcatcgacaa ccgtggcggc gccaacggca tactcggcgc cgacatcgtc gccaacgcca
      841 cgccggacgg ctacaccttt ctccatacgt ctttcgcctt cgcggtgaat ccgtccatcg
      901 tcatcaagat gccgttcgac gtcacgaggg atttcgtgcc ggtcacggat gtcgcgctgg
      961 gtaccggcta tctgatggtg gcgcacagca aatttgcccc caggacagtc aaggagttga
     1021 tcgacctggc gaagcagcag cccggtaaag tccggtacag cacggccggg gtcgggaacg
     1081 gacagcacct ggcgggagcg ctcttcgccg agaaagcggg catcgacctg ctccacgtgc
     1141 cctacaaggg gggcggcccg gcgatgacgg ctgcgctcgg cggagaaact cagctgcatt
     1201 ttcccgcacc cgccgtgggc attccccacg tgaagtcggg gagggtgagg gggctgggct
     1261 ttaccggcag caagcggctg atctcgctcc ccgacgtgcc cacgatcgga gaaaccgtga
     1321 agggctacgt cgccgacgcg ggatggcatg gcgtgtttgc gccggcgaag acaccgcccg
     1381 cgatcgtgaa aaaggtgcac gagtcgatcg cgaaagcgtt gaaagtgccg cacgttcgcg
     1441 accacttcgt caacgccggc tacgagccgc aaggcaatac cccggcggag tggaacaagc
     1501 gctttcgcgc ggacctgaag cgttacgcgg agattacgcg catcgcgaag atcgagcgcc
     1561 gttaattcgg aagcggaatt cccggacggc ggcgcatcgg gcgccgctgt ttttttgccc
     1621 ttcaggcccc cagtcgatcg aacagcccgg ctcccagctg cttgcggtac ttttccacca
     1681 ccggctgcaa agcccgcttg aacacggcgc gttcggtatc ggtgagttcg attacttcgt
     1741 tcattgcggg atcgagcttc ttcatgatct cggcgtcttc caccgctgct agttgccgct
     1801 ggtaggccgt tgcctccttc gccgccgcgt ctaccgcggc ctgcacgtcc ctgggccagc
     1861 cctggtattg cgcggcgttg cacacgagcg cgctcgcgcc ccagaaatgc gcagtgagcg
     1921 tgatatagcg atgatgagtg tggacgccga agttgtagat gttggtcagg ggattgtcct
     1981 gagcctggaa gcggtcgccg ccgacctgct ccacgaactc cttgatgtcg gacttgatcg
     2041 gctcgaagcc gagcgcccgg aacacttcgc cgtgcaggtc gctcatctgg gtgcggatac
     2101 ggatgccccg gcaatcctcg ggcttgcgga tcggccgcac ccggttcgag aggtaacgaa
     2161 aaccgttgtc ccagaatccc aggaacctgc agggcgtgtt tgcgtcgatc ttgcgcgcgg
     2221 cggcctgtcc aaattcgcca tccagcgcct cgaacacggt ggcgcgattc ttcacgacga
     2281 agggcagttc cagtatctgc agctccggcg ccactttcga aaagcgcacg gtggacatgt
     2341 agcagaacga cagttccccg ctctcgacca tcgggacgag atcgcccgac ccgcggccga
     2401 gcttgaggac gctgccgatc agctcgaagc ggatgcgctc accgagctgc ttcgtcaata
     2461 acccgccgaa gcgcccggca gcccggttgt ggatggaggc aggttcctgg tagccgccga
     2521 atttcaggac gatgggcgcg gtcatgcagg catttcgcga tgcacgaact cgatgatctt
     2581 ctcgatggcc gagcgcgcgg ccggcgacgc cggatcgttg gtgatgaacg cctcgcccat
     2641 gccttcgaac aggtgcagtt ccacacggcc gcccgccttg cggtaattcg cgatgaaacg
     2701 ctcgcggttg ggaaccgggt gcgccacgtc ggcggtgccc tgtaggtaga tcaccggcgg
     2761 cagcggtacc ttctcgccgc gctcgagcag gagcgtgggg ttgccttctg ccatggccgc
     2821 ctcgccgccc tgccagtact ggtcgtggca ttgaagccag ttgccggcct gcttcttgag
     2881 ggggccgttc tgactggact tggcgtattg atagcggccc aggggatcga tgaccggcca
     2941 gcacaggacc gcgcagcgca ccgttgcatt gatggcggcg tcggcggtct tgccggggag
     3001 gctcagcgcg gcgtagcgcg ggtcgttcgg gcgcagggcc gtcaacatcg cctggtggcc
     3061 gccactggag acgccgagta tgcccaccag gtccggccgc gtcttcaatt ccccggcccg
     3121 cgctttgcac cagcggatcg cgtaattcac gtcctgaaga gaggcggggt agccggcatc
     3181 gggcggcatg cggaagtcca gcgctaccac cgtgacgccg ctcctcgcca gcggctcgtc
     3241 ggttcccgcg tcgctcgtgc ggtccttgtt gcaccaggcg ccgccgtgca agtcgatgat
     3301 gagggggaac ggccccgccc cgcgcggctt gtagaggcgc gcgagcagcg gcttgtttcc
     3361 gtgccggata tattcgacgt cctcgacgtc gatgtcgtag cgggtgagcg tggcggttgt
     3421 catggcgggc tcctccaatg atagtgacgg gtgatggggg cgaagaaaac gccgcatccc
     3481 tcatccctga tgtttcaatt ccttcggggc gcccggctgt ctgagcgggc gcgacatgtc
     3541 gattcaagct ttcggaatga caggcagcag tatatacgag gggtgccggg gaccgacgtg
     3601 gagcgtgacc ttgccgccga acaccgatgc cgggcggtcg cgcgggtcgt cgtgcagaaa
     3661 agggccgcag ccgagcagct cgttcttgaa gttggagagt cttgcgccgg tggccttttg
     3721 ccattcgtaa tcgcgcccgc gcacgctcag cgcgatgcgg taaccggccg gcaccacgat
     3781 cgaggtgggc cagatctcga tatcgagctg gacaatctcg ccgggtgaga gcggctgctt
     3841 gcggtcgtgc gtgtggtagg gacgatattc agttgagagc ccggggtcga gctggcgatg
     3901 cgaagcgcgc agccaaccct gcgcgaccgg cgtatgcggg tctatcgctc ccatgaacac
     3961 gacttcccgg aggtcgggcg tgtaaacgcg gaagaccagg aagaggtcgg catcggcagt
     4021 cgaggaagag acgaacaact tcgccgccag cggcccggtg atctcggtct cccgttcgaa
     4081 cggcggcgac agaaaggtca cgccgtcgcc cagcccatcg taggagatgc tgccggccga
     4141 cccggcgggc tcgcgcgtga ggctgtgatt gcccgggtca aggtgaaacc gggtccactg
     4201 cgtgcgcgcg agcggccact cgctctcgtg gcgctcgacg aagcgatccg tgtggcgcac
     4261 ctggagctgc accctgggct ggcggtccca cccgttgttc tcgcccttca ggaagtagtc
     4321 gaagaaacgc ttctgcagga gccgcccgta atc
//
LOCUS       DBNGSG010000385         3153 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_295706, whole genome shotgun sequence.
ACCESSION   DBNGSG010000385 DBNGSG010000000
VERSION     DBNGSG010000385.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_295706"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="AC158Q_09940"
     CDS             complement(<1..789)
                     /locus_tag="AC158Q_09940"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3340138.1"
                     /translation="MAISPHSAQLPAPPAEALALSGQLARLIAEEIGRAGGWLSFARY
                     MELALYAPGLGYYMAGARKLGRDGDFVTAPEISRLFGRTLARQMQDFVELGLTDVLEI
                     GAGSGTLAADLLLELERLGCAPQRYLILELSPDLRDRSRDTLAARAPHLLERVAWLNR
                     LPPAFGGIVIGNEVLDAMPARVFRREGARIAELGVAARDGSFAWEARPPMPSSDLPDA
                     SCFPEDGYRTEFQFAARGFIRSLGGALARGAALFFDYGFPRHEYY"
     gene            855..1607
                     /locus_tag="AC158Q_09945"
     CDS             855..1607
                     /locus_tag="AC158Q_09945"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF006598.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HIR3340139.1"
                     /translation="MDDALQDKVVLVTGGAKRVGAAICRRLHAAGASLLVHYRSSAIE
                     ARALQVDLQARRADSVALAQADLLKTASSAQLVKAALNHFGRLDALVNNASSFFPTAV
                     GEITEQAWDDLIGTNLKAPLFLAQAAAPELKKNHGCIVNLIDIHAELPMRNHAVYTAA
                     KGGLAALTRALARDLGPEVRVNGVAPGTILWPDDDTWRDEVSRQRIINQSALKRIGEP
                     DDIARAVQFLLADAPYVTGQILTVDGGRGISL"
     gene            1763..2617
                     /gene="ttcA"
                     /locus_tag="AC158Q_09950"
                     /pseudo
     CDS             1763..2617
                     /gene="ttcA"
                     /locus_tag="AC158Q_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007747906.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
     gene            2731..>3153
                     /locus_tag="AC158Q_09955"
     CDS             2731..>3153
                     /locus_tag="AC158Q_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HIR3340140.1"
                     /translation="MNCAERLVRLFGSQAEVARRLRLDRAVVNNWIKSGYVPARWAME
                     VEALTSGQVSAADVLNEANARKPIRLKSRPDGESLFGSPLTGNDAMNDFAPVKRINSF
                     HPPQRTLMGPGPTEIHPRVLTTMSQPAIGYLDPVFVEMM"
BASE COUNT          599 a         1029 c         1044 g          481 t
ORIGIN      
        1 gtagtactcg tgccgcggaa agccgtagtc gaagaaaagc gccgcaccgc gcgcgagcgc
       61 gccgcccagg ctgcggatga atccgcgcgc ggcaaactgg aattcagtgc gatatccatc
      121 ttccggaaaa caggatgcat cgggaaggtc ggatgacggc atgggcgggc gggcttccca
      181 ggcgaacgag ccgtcccgcg ccgcgacacc cagctcggcg atgcgcgcgc cctcgcgccg
      241 gaacacgcgc gcgggcatcg cgtcgagcac ctcgttgccg atcacgatgc cgccaaaggc
      301 gggcgggagc cggttcagcc acgccacgcg ctcgagcagg tgcggcgcgc gcgcggcgag
      361 cgtgtcgcgc gagcggtcgc gcaggtcggg cgagagctcc agtatcaggt agcgctgcgg
      421 cgcgcagccg agccgctcga gttcgagcag caggtccgcg gcgagcgttc cgctgccggc
      481 gccgatttcc aggacatcgg tgaggccgag ctcgacgaaa tcctgcatct ggcgcgccag
      541 cgtgcggccg aacaggcggg aaatctccgg cgccgtgacg aaatcgccgt cgcgaccgag
      601 cttgcgcgcg ccggccatgt aatagcctag gccgggcgcg tagagcgcga gctccatgta
      661 gcgcgcgaag gagagccatc cgccggcgcg gccaatttcc tccgcgatca gccgggccag
      721 ctgcccgctc aaggcaagcg cctcggcggg cggggccggg agttgcgcgg aatgcgggga
      781 aatggccaag cggggtgccg cggaggaatg gaatacaatt ctacacgctc acgcaggcgg
      841 cacaggacgg cgaaatggac gatgcgttgc aggacaaggt ggtgctggtc accggcggcg
      901 cgaagcgcgt cggcgccgcg atttgccggc ggctgcacgc cgccggcgcg agcctgctcg
      961 tgcactaccg ctcctccgcc atcgaggcgc gcgcgctgca ggtagacctc caggcgcgcc
     1021 gcgccgattc ggtcgcactg gcgcaggcgg acctgctcaa gaccgcgagc agcgcccagc
     1081 tcgtcaaggc ggcgctgaat cacttcggtc gcctcgacgc gctcgtcaac aacgcctcga
     1141 gcttttttcc caccgcggtc ggcgagatca ccgagcaggc ctgggacgac ctgatcggca
     1201 ccaatctcaa ggcgccgctg tttctggccc aggctgcggc gccggagctc aagaagaatc
     1261 acggctgcat cgtcaacctg atcgacatcc acgcggagct gccgatgcgc aaccacgcgg
     1321 tttataccgc ggccaaaggc gggctcgcgg cgctgacgcg cgcgctggcg cgcgacctcg
     1381 ggcccgaagt gcgggtgaac ggcgtcgcgc ccggcaccat cctgtggccg gacgacgaca
     1441 cctggcgcga cgaagtgtcg cgccagcgca tcatcaacca gagcgcgttg aagcgcatcg
     1501 gcgagcccga cgacatcgcg cgggccgtgc aattcctgct cgcggacgcg ccttacgtca
     1561 ccgggcagat actcacggtg gacggcgggc ggggcatcag tctttgaaaa tgggtgactc
     1621 gttgctcgtt tctcgttact cgtcttttcc actcacgccg gccctctccc gcgagaggag
     1681 aggggggttg aagtaaacgt cgagaagcga aaaacgagaa acgagaagcg agaaacgaga
     1741 aacgaatcac tgggtgaata tcatggaaac gaccgtaata cgcgtcatgc cccgcagcag
     1801 gaaagaagag cggcagtcgt acgaggcgaa caagctcgtg aaacggctgc gccggcaggt
     1861 cggcgaggcg gtcgccgatt tcggcatgat ttccgacggc gacaaggtga tggtgtgcct
     1921 ctccgggggc aaggatagtt acgcgctgct gaacatcctg atgtacctgc gcgaacgggc
     1981 gccggtccgc ttcgacctcg tggcggtgaa tctcgaccag aagcagccgg gcttcccaga
     2041 gcacgtgctg cccgagtacc tcgcgcgcct gggggtgccg ttccacatcg agcagcagga
     2101 cacctacagc acggtgaagc gcgtcattcc agagggcagg acgatgtgct cgctgtgctc
     2161 gcggctgcgg cgcggcgtgc tctaccgcct ggccggcgag ctccacgcga ccaagatcgc
     2221 gctcggccac caccgcgacg atatcctcga gaccttcctg ctcaacatct tctacagcgg
     2281 gcagctgaag gcgatgccgc caaagctacg ttccgacgat ggccggcacg tggtgatccg
     2341 cccgctcgcc tactgcgagg agcgggacct cgccgcttat gccggtatga agcagttccc
     2401 catcatcccg tgcaacctgt gcggctcgca ggacaacctg aagcgaaacg agatgaagca
     2461 actcctgcgg gagtgggaga ggcgctatcc cggccgcgtg gagaccctgc tgaacagcct
     2521 gaagcatgtc cggtcatccc acctgctcga ccgcgaggcg tttgatttcg accggctgcc
     2581 gcccagccgt ggcgcgccgc gcgcgcagct gcaaggtact gaatagcaac gacttatagc
     2641 gtggctgttc gaataactta ctgatttata tgtatttaat agtatcaccc agcttgtcaa
     2701 agtaattacg tcatgctaat cttccgcgcc atgaattgcg cggaacgact cgtacgcctc
     2761 ttcggcagcc aggccgaggt cgcccgccgc ctgcggctcg accgcgcggt ggtcaacaac
     2821 tggatcaaat ccggctacgt gccggcgcgc tgggcgatgg aagtggaagc gctgacctcg
     2881 gggcaggtca gcgcggcaga cgtgctcaat gaagccaatg cccgcaagcc gattcgactt
     2941 aaatcgcgtc cggacgggga aagcctgttc ggctcgccac tcacggggaa cgacgccatg
     3001 aacgattttg cgccagtcaa acgcatcaat tccttccatc ctccgcagcg cacgctgatg
     3061 ggccccggcc cgaccgagat ccacccgcgc gtgctcacca cgatgagcca gccggccatc
     3121 ggctacctcg acccggtctt cgtcgagatg atg
//
LOCUS       DBNGSG010000386         4764 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1823330, whole genome shotgun sequence.
ACCESSION   DBNGSG010000386 DBNGSG010000000
VERSION     DBNGSG010000386.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4764)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4764)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4764
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1823330"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1223)
                     /locus_tag="AC158Q_09960"
     CDS             complement(<1..1223)
                     /locus_tag="AC158Q_09960"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR3340141.1"
                     /translation="MLGIAVVAPAAAPPVESRDPFDTGRFLPSSPGVRWQSPIELPRV
                     PEAPSAPVPSADRPLALPELTEFALQNNPRTRQAWFAARAAAAAIGIERSDDLPQIAA
                     TLSGQRSESGGQTGNQNPWLTRYGPAVTLSYLLFDFGAGDSRVQAAEYQALAAALTQN
                     RVLQDVVFQVEQAYFRVLGTDQLVRANELFLKSVSTSLDATQRRREGGLATVADVFRA
                     ETQVAQGRLNLTRSRGELEKARGSLASAVGLPVNAAIRVQTLESPPRIQEITESLNEI
                     LKRAKLNRPDLIAAEAQVRSARAQADAVARGTLPSITIDATTGRTFYDGQRPFTDTNS
                     VLLNLRIPIFTGFNHTYTVSQAEARAAQAEASRDILYRQSELEVWQSYYDVQTAADGI
                     SSTEVQLRAAQQTAEA"
     gene            1538..2707
                     /gene="sat"
                     /locus_tag="AC158Q_09965"
     CDS             1538..2707
                     /gene="sat"
                     /locus_tag="AC158Q_09965"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF003166.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HIR3340142.1"
                     /translation="MSGLVHPHGGGSLKALLLEGAGLADAIRQAQSLPRITVSSREKG
                     DLIMLGMGGFTPLDGFMGRADWEGVCDGYRMASGTFWPIPITLSADKSQAEAIKAGAE
                     IALMDPESGEPLATMKVAEKYAIDKARECKAVFKTDDAKHPGVAMVMAQGEVNLAGPV
                     KLLSDGGFKAKYGALFMTPAETRAVFQRLGWSRVAAFQTRNPMHRSHEYLAKVAIEVC
                     DGVLIHSLLGNLKAGDIPADVRTRAIALLVERYFRPGTVLQAGYPLDMRYAGPREALL
                     HALFRQNYGCSHLIVGRDHAGVGSYYGPFDAHKIFDEIPKGSLEIQALKMDVTFWCYK
                     CGGMASGRTCAHAAADQLQVSGTQLRKWLSEGSPVPAEFSRPEVLEVLREYYARA"
     gene            complement(3149..3658)
                     /locus_tag="AC158Q_09970"
     CDS             complement(3149..3658)
                     /locus_tag="AC158Q_09970"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR3340143.1"
                     /translation="MSETIRDGKFVELTYKVTDRKSGHVLTRVEFPLGYVHGHNEILA
                     PTVHRELEGKSPGDFIEVPIDGNQIFGARDESLVFTDHIENVPEEYRQLGTSILMEND
                     KGQTRSFIVTRIDDETLTIDGNNPLCGREVVFKLEILAVRDATDEETRAGGAISAGAD
                     IDPSLMRPV"
     gene            complement(3678..4064)
                     /locus_tag="AC158Q_09975"
     CDS             complement(3678..4064)
                     /locus_tag="AC158Q_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340144.1"
                     /translation="MKSVSSARGESRTNIIDKTDEEILAIADSLWRDLVKSSNEGKYG
                     EFARNFSNSLALAIDQVVAGHQFANSELARNLSEDFDSLGIIRRGEHVTVLYRQRSTK
                     KQGEWLGRIVLGYENGQVRIFGASIF"
     gene            complement(4076..>4764)
                     /locus_tag="AC158Q_09980"
     CDS             complement(4076..>4764)
                     /locus_tag="AC158Q_09980"
                     /inference="COORDINATES: protein motif:HMM:NF014907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340145.1"
                     /translation="SPPEYFWGYNRMTTIEGLFGAGDAVGGTPHAFSSGSFTEGRLAA
                     KAACKYIDDGKAEGIVVSDEQIGRRKAEIYKPLEHYRIYRNAIVAGSVNPHYINPQQG
                     LDRLQKLMDEYCGGVTVSYMTNEKLLNIGLKKLRIMEEDLENLAAKDTHELLRAWELK
                     HRHRAAECVTQHTLFRKETRWPGYYYRGDAMKVDDQNWHVLTVSRRDPDTGEYTMEKA
                     PCYHLVSDKE"
BASE COUNT          861 a         1508 c         1452 g          943 t
ORIGIN      
        1 gcttccgccg tctgctgggc agcccgcaac tgcacctcgg tcgagctaat cccgtcggcc
       61 gctgtctgta catcatagta ggactgccat acctccagtt ccgactggcg gtacagtatg
      121 tcgcgggatg cttccgcctg cgcggctctc gcttcggcct ggcttactgt ataggtatgg
      181 ttgaaaccag tgaagatcgg aattctcagg ttaagcagaa cactgttcgt gtccgtgaac
      241 ggacgctgcc cgtcgtaaaa agttcgtccc gtcgtcgcgt cgatcgtaat cgacggcagc
      301 gtgcccctcg cgaccgcatc ggcctgagcg cgtgccgagc gcacctgcgc ctccgccgcg
      361 atcaggtcgg gccggttgag cttggcgcgt ttaagtatct cgttcaggga ctcggtaatc
      421 tcctgtatgc gcggcgggct ttcgagagtc tggaccctga tcgccgcatt gaccggcaag
      481 cccacggcgg atgccagcga tcctcgcgcc ttttccaact cgccgcgact acgcgttaga
      541 ttgagccgcc cctgcgccac ctgcgtctcg gctcggaata catccgcgac cgtcgccagc
      601 ccgccctcgc gcctgcgctg ggtcgcatcc agcgaagtgc tcacgctctt caggaacaat
      661 tcgtttgccc gcaccagttg atcggtgccc agcacgcgga aataggcctg ctcgacctgg
      721 aacaccacgt cctgcagaac cctgttctga gtcaacgccg cggccaacgc ctggtattct
      781 gcagcctgaa cgcggctgtc cccggccccg aaatcaaaca aaagatagga caaggtcacg
      841 gcggggccgt agcgcgtgag ccaggggttc tgatttcccg tctggccacc cgattcgctg
      901 cgctgcccgc tcaacgtggc ggcgatctgg ggcagatcgt ccgagcgttc gatgccgatc
      961 gcggcagcgg cggcccgcgc ggcgaaccag gcctgccggg tcctcgggtt gttctgcagc
     1021 gcgaactccg tcagctccgg cagggcgagc ggccggtcgg cggacggcac aggcgcgctg
     1081 ggtgcctcag gaacccgggg cagctcgata ggggattgcc aacggacgcc aggggacgaa
     1141 ggcagaaagc gccccgtgtc gaaggggtcc ctggattcca ccggcggcgc agcggccggc
     1201 gcaacgaccg caatacccag caacagcgct cccagaaacg tcgagtacaa gaccagaata
     1261 agcccctcca agaacccgta ataaccgcta ctgccgggtc ctccgaatgc ttgttcttgt
     1321 gccggcgtca actgagcggg aaatcgtcag tcgcggcatc ataacatacc gttttttacg
     1381 cgctagtccg gctagttagg tgcgtctgcc cgcatcaaag gtcgctcgaa tcgaagaatc
     1441 ttatcccgca atagaattaa aaggctattc accggctgcc cgcgtgctaa cctcttgcct
     1501 ccaaacaaaa aaccttccct cgcccctgct ttagaccatg tccggcctag tccatcctca
     1561 cggtggtgga agcctcaagg cattgctgct cgagggagcg gggctcgccg acgccatccg
     1621 gcaggcgcag tccttgcccc gcatcactgt aagttcccgg gaaaaaggcg acctaatcat
     1681 gctcggcatg ggcggcttca cgccgctcga cggcttcatg ggccgcgccg actgggaagg
     1741 cgtgtgcgac ggctatcgaa tggcctcggg cacgttctgg ccaatcccga tcacgctgtc
     1801 cgcggacaag tcgcaggcgg aggcgatcaa ggccggcgcc gagattgcgc tcatggaccc
     1861 ggagagcgga gagccgctcg ctaccatgaa ggtcgcggag aagtacgcga tcgacaaagc
     1921 gcgcgagtgc aaggcggtgt tcaagacgga cgatgcgaag catcccggcg tcgcgatggt
     1981 catggcccag ggcgaagtga acctcgccgg cccggtgaag ttgctgtcgg atggcggatt
     2041 caaggcgaag tacggggcgc tcttcatgac gcccgccgag acgcgcgcgg tattccagcg
     2101 gctcggctgg tcgcgcgtcg cggcgttcca gacccgcaac ccgatgcacc gctcgcacga
     2161 gtacctcgcc aaggtcgcga tcgaagtctg cgacggcgtg ctgatccact ccctgctcgg
     2221 caatctgaag gccggcgaca tcccggccga cgtgcgcacg cgggcgatcg cgctgctcgt
     2281 cgagcgctat ttccgccccg gcaccgtgct ccaggcgggc tacccgctcg acatgcgcta
     2341 cgccggcccg cgcgaggcgc tgctgcacgc actgttccgc cagaactacg gctgctcgca
     2401 cctgatcgtc gggcgcgacc acgcgggcgt gggcagctac tacgggccgt tcgacgcgca
     2461 caagatcttc gacgaaatcc cgaagggttc gttggagatc caggcgctga agatggacgt
     2521 gaccttctgg tgctataagt gcggcggcat ggcctcggga cgcacctgcg cgcacgccgc
     2581 cgccgaccag ctgcaggtgt cggggacgca actgcgcaag tggctgtcgg aaggcagtcc
     2641 cgtgccggcg gagttcagcc ggcccgaggt gctggaagtg ctgcgcgagt actatgctcg
     2701 cgcgtagaag gattttcgcc cgttcgtcct gagcgagtcg aaggacgacg gcagctcacg
     2761 aatttcccaa acccgttcgt ggttcgacga gctcggcacg aatgggcgcc tattcggggc
     2821 gctagtcgac gatgtgatag accggcggct tctccatttg ggttcgaatc ctggtcgtgc
     2881 gattcgactc atcctgcgct atggccccta cggctgtttc ggcgtcttgt attcaacttc
     2941 cttctggaac ttttcccaga cggtttcgta accaacaaac cccttttcgt ttggctccat
     3001 ctgacgagtt gtcaaaaacg ggcatggccg tcaggaactt gggtggcggg tttttgctcg
     3061 ctcataagtc accttttctc tgtgaagtca ggattgcagg tgcaagcaac aaagctgaaa
     3121 ttcttccgat gccattcgac accgccgctc aaacaggccg catgagcgac gggtcgatat
     3181 ccgcgcccgc agagatcgcc ccacccgctc ttgtttcttc atcggttgcg tcgcgcacgg
     3241 ccagtatctc gagcttgaag acgacctccc tgccgcaaag cgggttgttg ccatcgatag
     3301 tcagcgtctc gtcgtctatc cgcgtcacga tgaagctgcg ggtctggcct ttgtcgtttt
     3361 ccatgaggat ggaagtaccg agctgccgat actcctcggg gacgttctcg atgtgatcgg
     3421 taaaaaccag cgactcatcc ctggcgccga aaatctggtt gccgtcgatt ggcacttcga
     3481 tgaagtcgcc gggggacttg ccctccagct cccgatggac ggtaggggcc aggatttcat
     3541 tgtgcccgtg aacatagccc aacgggaatt cgacccgggt gaggacgtgc cccgacttcc
     3601 tgtcggtcac cttgtaggtc aactcgacaa acttgccgtc ccggatggtt tcactcatgc
     3661 gaacccggac gagccgatca gaagatggat gcgccaaaaa tcctgacctg tccattctca
     3721 tagccgagga cgattcggcc aagccattcg ccttgcttct tggtgctgcg ctgccggtaa
     3781 agcaccgtca catgctctcc gcggcggatg atgccgagcg agtcgaagtc ctcggacaga
     3841 ttcctcgcga gttcactgtt tgcgaactgg tggcccgcca ccacctgatc gatggcaagc
     3901 gccagggagt tggagaagtt ccttgcgaac tccccgtatt tgccttcgtt cgaagacttg
     3961 acgaggtcgc gccacaggga atcggcgatg gcgagaatct cctcgtcggt cttgtcaatg
     4021 atgttcgtgc gcgactctcc gcgagcggaa gagaccgact tcatggggga cgatgctact
     4081 ccttgtccga aaccaggtga tagcaaggcg ccttctccat ggtgtattcc ccggtatccg
     4141 gatcgcgacg cgaaacggtc agcacgtgcc agttctgatc gtccaccttc atggcatcgc
     4201 cccggtagta gtagcccggc cagcgggtct ccttgcggaa cagcgtgtgc tgcgtgacgc
     4261 actccgcggc gcgatgacgg tgcttcaatt cccacgcgcg cagcaactcg tgagtatcct
     4321 tcgcggccag gttctcgagg tcttcctcca tgatcctgag cttcttgagg ccgatattca
     4381 ggagcttctc gttggtcatg tagctgacgg tcacgccgcc gcaatactcg tccatcagct
     4441 tctgcagacg gtccaggccc tgctggggat tgatgtagtg cgggttgacg ctgcccgcca
     4501 cgatcgcgtt gcggtagatc ctgtaatgct ccaggggctt gtagatctct gccttgcggc
     4561 ggccgatctg ctcatcggat acgacgatgc cttcggcctt gccgtcgtcg atgtacttgc
     4621 aggcggcctt cgcggcgaga cggccttcgg tgaacgaccc cgacgagaag gcgtgcggcg
     4681 tgccgccgac cgcatcgccg gcgccgaaca ggccttcgat cgtcgtcatg cggttgtagc
     4741 cccagaaata ctccggaggg gaaa
//
LOCUS       DBNGSG010000387         4349 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1993924, whole genome shotgun sequence.
ACCESSION   DBNGSG010000387 DBNGSG010000000
VERSION     DBNGSG010000387.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1993924"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(250..471)
                     /locus_tag="AC158Q_09985"
     CDS             complement(250..471)
                     /locus_tag="AC158Q_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding carrier protein"
                     /protein_id="HIR3340146.1"
                     /translation="MARIEIKSEITGTVWQVKSKPGDKVESGDVLVVIESMKMEIPVI
                     SEDAGTVKEVLVKEKDPVSEGQVVAVLDG"
     gene            598..1407
                     /locus_tag="AC158Q_09990"
     CDS             598..1407
                     /locus_tag="AC158Q_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006758509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR3340147.1"
                     /translation="MSGLPALKDAALELDGRVAVLVFKRDDMRNALSGTHLTEDICAT
                     LEWANASPGVSVLILTGDGSAFSAGGNIKAMRNRAMTSSAAEMERYYRYGIQRIPRAM
                     NAAEIPVIAAVNGPAIGAGFDLVNMCDIRIGSSDAQFGETFINLGLMAGDGGGWFLRR
                     AVGAQRAAELTLTGRVIKAEEARALGILLEVTEPGRLLERAKEIAAQIASRPPLAVRY
                     AKRALRLAQHQGLEEHLDSCASFQAALQKTGDHLEALSAFFEKRSGVFKGE"
     gene            1404..2093
                     /locus_tag="AC158Q_09995"
     CDS             1404..2093
                     /locus_tag="AC158Q_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340148.1"
                     /translation="MILRFVLRLLLVILVMAAYAPPLRSQERPLDPMDEAAKDASWRS
                     FKENLLAALEKRDRDFVLGILDAEVRSGQAGERGVAAFKEEWGLESDTGPLWKELAAV
                     LALPAAYHRPEQGPLELCAPYVAVRWPQDIDAYKGGAIVVKSALVKSAPSSAAGTIAT
                     LSYHLVEVTDWEVNDRSADSKQKWVKIRLRAGEGYVPEEQIRSPIEHTACFVKGERGW
                     RLVGFGPGGGK"
     gene            2187..3131
                     /locus_tag="AC158Q_10000"
     CDS             2187..3131
                     /locus_tag="AC158Q_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HIR3340149.1"
                     /translation="MTAASGQALDLQGHRGARGLAPENTLPAFARALSIGVTTLELDC
                     AITKDGVVVVSHDSTLNPDITRGPDGQWLQGVGPPIRNFTYDELQRYDVGRIKPGSAY
                     ERRFPQQRAIDGTRMPRLADVFALARQGANEQVRFNIETKISPLHPGRTVSPEMFARA
                     LIAVIRGEEMQRRVTIQSFDWRTLAVVQKEAPEITTVYLTAQQGFMDNIQAGQRESAW
                     TAGLRLHDFGGSIPRMVKAAGGAVWSPYSGETTREQVREAQSLGLKVVVWTVNEPADM
                     RRMIEMGVDGVISDRPDLLRKVAVEMGLALPPATPVAP"
     gene            complement(3138..3518)
                     /locus_tag="AC158Q_10005"
     CDS             complement(3138..3518)
                     /locus_tag="AC158Q_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3340150.1"
                     /translation="MLTHAPVTTLLPVKDLNRACEFYEKKLGLKPLGSRPDGKFQFAG
                     GSGGVIALFPKPEGTKAEHTAVSFQVKDIGKEIQELKQRGVVFEDYDFPGLKTVNHVC
                     VLGAEKAAWFKDTEGNYLCIHEDI"
     gene            complement(3548..>4349)
                     /locus_tag="AC158Q_10010"
     CDS             complement(3548..>4349)
                     /locus_tag="AC158Q_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HIR3340151.1"
                     /translation="AHSMEAPPVQKVPAGSGEACRRMLEIVPEARNKVYDVRKVIAAI
                     ADKGSVFELKERYGRSLATVLARLDGKSVGFIANNPMFKGGALDADACQKATSFMVLC
                     DSFNIPIVFLVDVPGFLIGVEGELRGMPGKVINWMNALSLVTVPRVTVIMRKSYGQAF
                     INMGGGRNTDELACWPSADLGFMDPRVSVNVLYGVKEQDDPERFRQLVAEVNRDTTPW
                     ELARLYEAQHVIDPRDTRDFLIRMLAVHRQRLRNGLGQHRLANWPTSY"
BASE COUNT          766 a         1391 c         1419 g          773 t
ORIGIN      
        1 cagcaatcgg tcggcaactg gcggcggctg gtgcaagcgg tgcgggaagg cgacgggctg
       61 gaagcccagc gcgcggcgga gcagagggtg ctggattcgc gcgatgccgc tatcgcggaa
      121 ttgcaaaagg cgtgaatcgt gattggtgaa tggaacgcta cgttgaagct gctttgcccg
      181 ctgcgcgaaa gcgccgaggc aagtaaatcg attcaccact caccattcac cattcacgca
      241 gttgaggcat cagccgtcga gaacggcgac gacctggcct tcggagaccg ggtccttttc
      301 cttcaccagg acttccttca ccgtgccggc gtcctcgctg atcaccggga tttccatctt
      361 catggactcg atcacgacca ggacgtcgcc ggactcgacc ttgtcgccgg gcttgctctt
      421 cacctgccac acggtgccgg tgatttcgga tttgatttcg atgcgcgcca tgctgttgcc
      481 cgtcgaccaa gagattgctg aatgggattt gccgggttac ttgaaaaatc cgcgaggagt
      541 atatcacggc ggtttgcgcc gccctcgtcg ccgcataacg gttaaaatcc agccatcatg
      601 tcagggcttc ccgcactcaa ggacgccgcc ctcgagctgg atgggcgcgt cgccgtgctc
      661 gttttcaagc gcgacgacat gcgcaacgcg ctctccggca cccatctcac cgaggacatc
      721 tgcgccacgc tcgaatgggc gaacgcgagt ccgggcgttt cggtgttgat cctgaccggg
      781 gacggctcgg cgttctcggc cggcggcaac atcaaggcca tgcgcaatcg cgccatgacc
      841 tccagcgcgg ccgagatgga gcgttactac cgctacggca tccagcgcat cccgcgcgcg
      901 atgaacgccg ccgagatccc cgtcatcgcg gccgtcaacg ggccggcgat cggcgcgggt
      961 ttcgatctcg tcaacatgtg cgacatccgc atcggctcct ccgacgccca gttcggcgaa
     1021 actttcatca acctgggcct catggcgggc gacgggggcg ggtggtttct gcggcgcgcg
     1081 gtcggcgcgc agcgcgccgc cgaactgaca ctcaccgggc gcgtcatcaa ggccgaggag
     1141 gcgagggcgc tcggcatcct gctggaagtc accgagcccg gccgcctgct cgagcgcgcg
     1201 aaggagatcg ccgcgcagat cgcgtccagg ccgccgctcg ccgtgcgcta cgccaagcgc
     1261 gcgctgcgcc tcgcgcaaca ccagggcctg gaggagcacc tggattcctg cgcttcgttc
     1321 caggcggcgc tgcagaaaac cggggaccac ctcgaggcat tgtcggcttt tttcgaaaaa
     1381 cgcagcggcg tattcaaggg agaatgatct tgcgattcgt cctgcgcctg ttgttggtga
     1441 ttctggtcat ggcggcatac gccccgccgc tgcgttccca ggaacgcccg ctcgacccga
     1501 tggacgaggc ggccaaggac gcgtcgtgga ggagcttcaa ggaaaatctg ctggccgcgc
     1561 tcgagaagcg cgaccgcgat ttcgtgctgg gcattctgga tgccgaagtc cgcagtggcc
     1621 aggcgggcga gcgcggggtt gccgcattca aggaagagtg ggggcttgaa tccgatacag
     1681 gcccgctatg gaaggagctg gctgcggtgc tggcgctgcc agccgcctat caccggccgg
     1741 agcagggacc gctcgaactg tgcgcgcctt acgtagcggt gaggtggccg caggacatcg
     1801 acgcttacaa gggcggcgcg atcgtggtca agtcggcgtt ggtgaaatcg gcgccgtcgt
     1861 ccgccgccgg cacgatcgcg accctgtcct accatttggt cgaggtaacc gattgggagg
     1921 tcaacgaccg gtcggcggat tcgaagcaga agtgggtcaa gatcaggctc cgggcggggg
     1981 agggttacgt gccggaagaa cagatccgca gcccgatcga acacaccgcc tgcttcgtca
     2041 agggcgagcg cggctggcgg ctcgtggggt tcggcccggg cggggggaag tgagggatga
     2101 gggatgagga tttgtccggg tcgaatcacg actcacaact cacgaatcac gcttttttcc
     2161 gcactctact ggccatcgcc ctggcgatga ccgccgcatc cggccaggcg ctcgacctgc
     2221 agggccaccg cggcgcgcgc gggctcgcgc ccgaaaatac cctgcccgcg ttcgcccgcg
     2281 cgctttcgat cggcgtcacc acgctggaac tcgactgcgc gatcacgaaa gacggcgtcg
     2341 tagtggtgag ccacgactcc acgctgaacc ccgacatcac ccgcggcccg gatggtcaat
     2401 ggctgcaagg cgtggggccg ccgatccgga acttcaccta cgacgagttg cagcgctacg
     2461 acgtcggccg gatcaagccc ggcagcgcct acgaaagacg gttcccgcag caacgggcga
     2521 tcgacggcac gcgaatgccg aggctggcgg atgttttcgc gctcgcgcgc caaggcgcca
     2581 acgagcaggt gcgcttcaac atcgagacca agatctcacc cctgcacccc gggcgcacgg
     2641 tttctccgga aatgttcgca agagcgctca tcgcggtcat tcgcggggaa gaaatgcagc
     2701 ggcgcgttac gatacagtcc ttcgactggc gcacgctcgc agtcgtgcag aaggaggcgc
     2761 cggaaatcac cactgtctat ctcaccgcgc agcaaggctt catggacaat attcaggccg
     2821 gacagcggga gtctgcctgg acggcgggcc tgcgcctgca cgatttcggc ggctcgatcc
     2881 cgcgcatggt gaaggcggcg ggcggggccg tgtggtcgcc ctactcggga gagaccaccc
     2941 gcgagcaggt gcgggaggcc cagtcgctcg gcctgaaggt cgtggtatgg accgtaaacg
     3001 agcccgccga catgcgccgc atgatcgaga tgggcgtaga cggcgtgatc agcgaccgtc
     3061 ccgacctgct gcgcaaggtg gcggtggaga tgggccttgc cttgccgccc gcgaccccgg
     3121 ttgcgccctg acttgcgtca gatgtcttca tggatacaga ggtagttccc ttcggtatcc
     3181 ttgaaccagg ccgccttctc tgcgcccagc acgcagacat gattgacggt tttcagcccg
     3241 ggaaaatcgt agtcctcgaa cacgacgccc cgctgcttga gttcctggat ttccttgccg
     3301 atgtctttca cctggaagct caccgccgta tgctcggcct tggtcccttc gggcttcgga
     3361 aacagcgcga tgacgccgcc gctgccgccg gcgaactgga atttgccgtc cggcctggag
     3421 cccagcggct tcagccccag cttcttctcg tagaactcgc acgcccggtt gagatctttc
     3481 accggcagca gggtagtcac tggtgcatgg gtcagcatgg tgtcagctcc tttgcgtcac
     3541 acgtcactca ataactggtg ggccagttgg ctagtctatg ttggcccagc ccgtttctga
     3601 gtcgctggcg gtgcacggcg agcatgcgga tgaggaagtc ccgcgtgtcg cgcgggtcga
     3661 tgacatgctg cgcttcgtag aggcgcgcga gttcccaagg cgtggtatcg cggttgacct
     3721 cggcgacgag ctgcctgaaa cgctcggggt cgtcctgctc cttcaccccg tagaggacgt
     3781 tgaccgagac ccgcggatcc atgaagccca ggtcggcgga cggccagcag gccagctcgt
     3841 cggtgtttcg gccgccgccc atgttgataa acgcctggcc atagctcttc ctcatgatga
     3901 cggtgacccg cggcacggtg accagcgaga gggcgttcat ccagttgatg accttgcccg
     3961 gcatgccgcg cagctcgccc tcgacgccga tcaggaaacc cggcacgtcc accaggaaca
     4021 cgatgggaat gttgaaggaa tcgcacagca ccatgaaact ggtggctttc tggcaggcgt
     4081 cggcatcgag cgcgccgccc ttgaacatcg ggttgttggc gatgaaaccg accgacttgc
     4141 cgtcgagacg cgcgagcacc gtggcgagcg agcggccgta gcgctccttt aattcgaaaa
     4201 cggaaccctt gtccgcaatc gccgcgatca ccttgcgcac gtcgtacacc ttgttgcgcg
     4261 cctccggcac gatctcgagc atcctgcggc acgcctcgcc ggagccggcc ggcacctttt
     4321 gcacgggcgg agcctccatg ctgtgcgcc
//
LOCUS       DBNGSG010000388         4787 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_296069, whole genome shotgun sequence.
ACCESSION   DBNGSG010000388 DBNGSG010000000
VERSION     DBNGSG010000388.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4787)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4787)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4787
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_296069"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..132)
                     /locus_tag="AC158Q_10015"
     CDS             complement(<1..132)
                     /locus_tag="AC158Q_10015"
                     /inference="COORDINATES: protein motif:HMM:NF014666.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HIR3340152.1"
                     /translation="MIVRGSDRVLVMGITGKQGTFWTERMQAYGTKVVGGTNPGKAGT
                     "
     gene            complement(129..1268)
                     /locus_tag="AC158Q_10020"
     CDS             complement(129..1268)
                     /locus_tag="AC158Q_10020"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024938.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042709 - succinate-CoA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004774 - succinate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit beta"
                     /protein_id="HIR3340153.1"
                     /translation="MNFEEHAAKPLLARHGIAVPRGALAKTPGEAGEIAARLGAVVVK
                     AQVPTGKRGKAGGIKLAATPDEARAHARAIIGMKIAGHKVAKVLVEEQLPIERELYAA
                     VLTDPPSKGPLVMFSTEGGMDIEDVAATAPGKLRRQTVDIRHGFSAAAARKLVQGLGL
                     DAAEGRIAEALAGLYAAYAAFDAELLEINPLIVTRDGRVVALDCKFVMDDCGIVRQPG
                     VAPAGTPDELTGLEARAQAAGLKYIDLEGDIAVLANGAGLTMTTMDVVRHHGGRPANF
                     LEIGGESYTQARPALELLLSNPRIKSLVINFCGAFARTDVMTQGVIEAIEALEPKLPI
                     FFSVHGTGDEEAIRMLKERMGVTPLPTMDDACKAAVQAAKSTEAR"
     gene            complement(1480..2907)
                     /gene="sauS"
                     /locus_tag="AC158Q_10025"
     CDS             complement(1480..2907)
                     /gene="sauS"
                     /locus_tag="AC158Q_10025"
                     /EC_number="1.2.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylating sulfoacetaldehyde dehydrogenase"
                     /protein_id="HIR3340154.1"
                     /translation="MKMHSEPGARTTAVDIVAALVANARAAQKIYGRSTQAQLDEAVT
                     AAGWAIRNPDHNRALAELAVKDTGLGVAADKLVKNHRKTLGLLRDLKGAKSTGVIAEY
                     PERGITEIAHPVGVVAAAVPSTNPAATPANNIINALKCGNAVIVAPSPKGYSTCALLL
                     QYMHAELKRAGAPGDLVQMLPAPVTRELTNELMKQADLVVVTGSQNNVRAGYSSGTPA
                     LGVGTGNVAVIVDESADCADAAKKIMASKIFDNATSCSSENAVIIHDKVYDRMLAALA
                     KEGGALLTSPEKSTLQKAMFPGGKLSPAVTAQSVQKICGVAGLARAGLADAKCLMVEE
                     SGAGREHPYSGEKLSPVLAVYRARDFDHACGILGAIYDYQGNGHSCGIYTEDEAHIRR
                     LAHEMTVARVIVKQAHTFATGGSFDNGLPFSLSMGCGTWGGNSFSDNLNYRHFMNITR
                     IVRTIPPHEPTVDDIFGAYRSKHGL"
     gene            3126..4550
                     /locus_tag="AC158Q_10030"
     CDS             3126..4550
                     /locus_tag="AC158Q_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homospermidine synthase"
                     /protein_id="HIR3340155.1"
                     /translation="MDEKKHVRFQGRLVFVGFGSIGQGTLPLILRHVDMPRERITILT
                     ADDRGQKEAEQYGIRFVINPITRDNVKQVLQPMLGKGDFLLNLSVDVSSPVLIEYCMQ
                     NGVHYLDTCIEPWHGMYTDGSLSLASRSNYGLREDALAIGRKYPGAATVVLTHGANPG
                     LISHWVKQGMINIARDVSGGDVKIPKTREEWGRLAMKLGIKVIHCAERDTQVAEPRKQ
                     RNEFANTWSVDGFVSEGRQPAELGWGTHEKHFPRDGQEHDFGCRSAIYLKRPGMSTKV
                     RSWTPDEGSYHGFLITHGEAISIADYFTLTENGKAIYRPTCHYAYHPCDDAVLSNHEM
                     GGKNWIAQPKYRIFMDEIFDGVDELGALLMGHPKGAYWYGSRLTIQEARKLAPYNSAT
                     SLQVVVGVLGGLVWALENPDRGMVEPDDMDYRRVLDIADPYLGDLVGKYSDWTPLKDR
                     GWLYDEDEDIDNSDPWQFKNFRVV"
BASE COUNT          787 a         1503 c         1594 g          903 t
ORIGIN      
        1 ggttcccgcc ttgcccgggt tcgtgccgcc gacgaccttc gtgccgtagg cctgcatgcg
       61 ctcggtccaa aacgtgccct gcttgccggt gatgcccatc accagcacgc gatcgctgcc
      121 gcggacgatc atcgcgcctc cgtggatttc gcggcctgca ctgccgcctt gcaggcgtcg
      181 tccatggtcg ggagcggcgt gacgcccatg cgctccttca gcatgcgtat cgcttcctcg
      241 tcgccggtgc cgtggaccga gaagaagatc ggcagcttcg gctccaacgc ctcgatcgcc
      301 tcgatcacgc cctgggtcat cacgtcggtg cgcgcgaagg cgccgcagaa gttgatgacc
      361 aggctcttga tgcgcgggtt cgacagcagc agttccagcg cgggcctcgc ctgggtatag
      421 gattcaccgc cgatctcgag aaagttcgcc gggcgcccgc cgtggtggcg cacgacgtcc
      481 atggtggtca tggtgagacc ggcgccgttg gcgagcacgg cgatgtcgcc ttcgagatcg
      541 atgtacttga gcccggcggc ctgggccctc gcctcgaggc cggtcagctc gtcgggcgta
      601 cctgccggcg cgacgcccgg ctggcggacg atgccgcaat cgtccatcac gaacttgcaa
      661 tcgagcgcca cgacgcgtcc gtcccgggtg acgatgagcg ggttgatctc caggagttcg
      721 gcatcgaagg cggcataggc cgcatagaga ccggcgagcg cctcggcgat cctgccctcg
      781 gctgcgtcga gccccaggcc ttgcaccagc ttccgcgccg ccgcggcgga gaagccgtgg
      841 cgaatgtcga ccgtctggcg gcgcagcttg ccgggcgccg ttgctgccac atcctcgata
      901 tccatgccgc cttcggtcga gaacatcacc agcgggccct tgctcggcgg atcggtgagt
      961 accgccgcgt acagctcgcg ctcgatcggc agctgctcct cgaccaggac ctttgcgacc
     1021 ttgtgaccgg cgatcttcat gccgatgatc gcccgggcat gggcgcgggc ttcatccggc
     1081 gtcgcggcga gcttgatgcc gcccgccttg ccgcgtttgc cggtgggaac ttgcgccttg
     1141 acgacgacgg cgccgagccg cgcagcgatc tcgccggctt cgccgggagt tttcgcgagc
     1201 gcgccccgcg gcacggcgat accgtggcgg gccaggagcg gcttggccgc gtgttcctcg
     1261 aagttcatcg tctatcctaa ttgatgaaaa gctgaaccac agaggacacg gaggacacag
     1321 agggaatcat acaaaaatca tcagatcgaa ctcccgggat aaggaaaatg ctatgcgagt
     1381 tttcccgttc ggtcattcat gcctgagtct tcgggttttg atctccgtga atatggtttg
     1441 cattccttcg tgtcctccgt gtcctctgtg tcaaggctgt caaaggccgt gcttgctgcg
     1501 ataggcgccg aagatatcgt ccaccgtcgg ctcgtgcggc gggatggtgc gcacaatgcg
     1561 ggtgatgttc atgaagtgcc tgtagttcag gttgtcggag aagctgttgc cgccccaggt
     1621 gccgcagccc atggagagcg agaacggcag gccgttgtcg aagctgccgc cggtggcgaa
     1681 ggtatgcgcc tgtttcacaa tcacgcgcgc gaccgtcatc tcgtgcgcca ggcgccggat
     1741 gtgcgcctcg tcttcggtgt agatgccgca ggagtggccg ttgccctggt agtcgtagat
     1801 ggcgccgagt atgccgcagg cgtggtcgaa gtcgcgcgcg cggtagacgg ccagcaccgg
     1861 agagagtttt tcgccggagt agggatgctc ccgtcccgcg ccggattcct ccaccatcag
     1921 gcacttcgcg tccgccagcc cggcacgggc gaggcccgcc acgccgcaga ttttctgcac
     1981 cgattgcgcg gtcacggcgg gcgagagctt gccgccgggg aacatggcct tctgcaaggt
     2041 ggatttttcg ggcgaggtga gcagcgcgcc gccttctttc gcaagcgccg cgagcatgcg
     2101 gtcatagacc ttgtcgtgga tgatgacggc gttctcggac gagcagctgg tcgcattgtc
     2161 gaagatcttg gaggccatga ttttcttcgc ggcatcggcg cagtcggcgc tctcgtccac
     2221 gatgaccgcg acgttgccgg tgccgacgcc cagcgccggg gtgccgctgg aatagcccgc
     2281 gcgcacgttg ttctgcgagc cggtcacgac caccagatcg gcctgcttca tgagttcgtt
     2341 cgtgagctcc cgcgtaaccg gcgcgggcag catctggacc aggtcgccgg gggcgccggc
     2401 gcgcttcagc tccgcatgca tatattggag cagcagggcg caggtgctgt agcccttggg
     2461 cgagggtgcg acgatcacgg cgttgccgca cttcagcgcg ttgatgatgt tgttggctgg
     2521 ggtggccgcc gggttggtcg agggcaccgc ggccgccacc acgcccaccg ggtgcgcgat
     2581 ctcggtgatg ccccgttccg ggtattcggc gatgacgccg gtggatttcg cgcccttgag
     2641 gtcgcgcagc aggccgagcg tcttgcggtg gttcttgacg agcttgtcgg cggcgacgcc
     2701 cagccccgtg tccttcacgg cgagctcggc gagcgcccgg ttgtgatcgg ggttcctgat
     2761 cgcccagccg gcggcggtca cggcctcgtc gagctgcgcc tgggtcgagc gcccgtaaat
     2821 tttctgcgcg gcgcgagcat ttgcgacgag cgctgccacg atgtccacgg cggtggtgcg
     2881 cgcgcccggt tcgctgtgca ttttcatcga ctatcctgtg cgtgtagctg gtcaaagtcc
     2941 cgatgatacc gcgtgccgcg ggtttgccgg agttgatccg gcaagcgcat agtttatcgc
     3001 ggccgcagtc acgggatttg ctgccgttga tgcagtgcgt cggcatgcgc gccggagggg
     3061 cgacgcatgg aaaataaaat gctagactcc gattcagaaa aatgacagcc atccatcgag
     3121 gggaaatgga cgaaaaaaag cacgtcaggt ttcagggaag gctggtcttc gtcggtttcg
     3181 gttccatcgg tcagggcacg ctgccgctga tcctgcggca cgtcgacatg ccgcgggagc
     3241 gcatcaccat actcaccgcc gacgatcggg gccagaagga ggccgagcag tacggcatcc
     3301 ggttcgtcat caatcccatc acccgcgaca acgtcaagca ggtcctccag ccgatgctgg
     3361 gcaagggcga tttcctgctc aacctgtcgg tggacgtctc cagcccggtg ctgatcgagt
     3421 actgcatgca gaacggcgtg cactacctcg atacctgcat cgagccctgg catggcatgt
     3481 ataccgacgg ctcgctgtcg ctcgcgagcc gctccaacta cggcctgcgc gaggatgcgc
     3541 tcgcgatcgg caggaaatat cccggggcgg ctaccgtcgt cctcacccac ggggccaatc
     3601 cgggcctcat ctcgcactgg gtcaagcagg gcatgatcaa catcgcccgc gacgtgtcgg
     3661 gcggggacgt gaagattccg aagacgcggg aagagtgggg acgcttggcc atgaagctcg
     3721 gcatcaaggt cattcactgc gccgagcgtg acacccaggt cgccgagccg cgcaagcagc
     3781 ggaacgagtt cgccaatacc tggtcggtgg acggcttcgt cagcgagggc cgccagcccg
     3841 cggagctcgg ctggggcacc cacgagaagc atttcccgcg cgacggccag gagcacgatt
     3901 ttggctgccg ctcggcgatt tatctcaagc gtccgggcat gtcgaccaag gtgcgctcgt
     3961 ggacgccgga cgaggggtcg taccacggct tcctgatcac ccacggcgag gcgatctcga
     4021 tcgcggatta cttcaccctg accgagaacg gtaaggcgat ctaccgcccg acctgccact
     4081 acgcctacca cccgtgcgac gacgcggtgc tgtcgaacca cgagatgggc ggcaagaact
     4141 ggatcgccca gcccaagtac cgcattttca tggacgagat cttcgacggc gtggacgagc
     4201 tgggcgcgct gctgatgggg cacccgaagg gcgcgtactg gtacggctcg cggctcacga
     4261 tccaggaggc gcgcaagctt gcgccgtaca acagcgcgac cagcctgcag gtcgtggtcg
     4321 gcgtgctggg cgggctggtg tgggcgctgg agaacccgga tcgcggcatg gtggaaccgg
     4381 acgacatgga ttaccggcgc gtgctcgaca tcgccgatcc ctatctcggc gacctcgtcg
     4441 gcaaatattc cgactggacg ccgttgaagg accgcggctg gctttacgac gaagacgagg
     4501 acatcgacaa ttcggacccg tggcagttca agaatttccg cgtcgtctga cgcgggaatt
     4561 cagtgacgag tgatgagtga cgagagccgg gcgcaaggcg cctgacagaa gaacgggcac
     4621 gtgtgtgtgc ccgttctttt tgcctatcgc cggcttcaag ccctagtcac tcgtcacccg
     4681 tcacttgtca tgtctcattc gatttttatt ttggcgtcct tgacgatgcg agcgtatttc
     4741 gccgtctcgg agcggatgaa cgcgccgaat tgatccgccg tgttgcc
//
LOCUS       DBNGSG010000389         9253 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3521882, whole genome shotgun sequence.
ACCESSION   DBNGSG010000389 DBNGSG010000000
VERSION     DBNGSG010000389.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9253)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9253)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9253
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3521882"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..936
                     /locus_tag="AC158Q_10035"
     CDS             115..936
                     /locus_tag="AC158Q_10035"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HIR3340156.1"
                     /translation="MDLALYLKVVVLGIVEGLTEFLPISSTGHLIIAGQLLGFGGDKA
                     KVFDIAIQSGAMLAVVWEYRARFARVLTGLAGDPVARRFAANLVIAFLPAALLGLAFS
                     GAIKQYLFHAVPVALAFIIGGLVILWVERRPRRTTVEEVDDMTWKDALKVGLAQSVAL
                     IPGTSRSGATIIGGMLFGLSRRAATEFSFFLAVPTLIAAGAYDLYQHRALLDAADLGP
                     FGVGFAVSFVSAFACVRWLLRYIATHDFSVFAWYRIVFGVIVLVTAYTGLVNWTA"
     gene            complement(1025..1474)
                     /locus_tag="AC158Q_10040"
     CDS             complement(1025..1474)
                     /locus_tag="AC158Q_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008330337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR3340157.1"
                     /translation="MAIDDILRKAAERARQMGLSYGGALTPSEAHELWRNAPGAKLVD
                     VRTRAEWDYVGRIPGAVEIELLTYPANRPNPAFVAELESQVDKAAPVLFICRSGGRSH
                     NAAMLATQAGYSAAYNVLEGFEGDRDAQGHRNTVGGWRAAGLPWTQG"
     gene            1770..3182
                     /gene="glnA"
                     /locus_tag="AC158Q_10045"
     CDS             1770..3182
                     /gene="glnA"
                     /locus_tag="AC158Q_10045"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HIR3340158.1"
                     /translation="MATVADVLKMVKDNEVKFVDFRFTDTRGKEQHVSVPVSAFGEAK
                     FTDGHFFDGSSIAGWKGIEASDMTLMPDPDTARMDPFTDEPTLLLTCDVIEPSDGKGY
                     DRDPRSIAKRGEAYLKSTGIADTAFFGPEPEFFIFDSVTWKVEMGQAFYKIRSEEAPW
                     STGEEFEGGNMGHRPPVKGGYFPVPPLDSLQDIRSAMVLAIQEMGVPCEVHHHEVAAA
                     GQCEIGTLFAPLVKRADWLMILKYAVHNVAHSYGKTATFMPKPVVGDNGSGMHVHQSL
                     AKGGQNLFSGNGYAGLSELALHYIGGILKHAKALNAITNPGTNSYKRLVPGFEAPINL
                     AYSSRNRSASVRVPLVSNPKARRIEVRFPDPTTNPYLAFTAIMMAGLDGIQNKIKPGD
                     PIDKNLYDLPPEIAAKVPKVCHSLEQALEELEKDHEFLLRGGVFSRDMLDAYVALKHE
                     ELTAFRMTTHPLEFQMYYSL"
     gene            3368..3841
                     /locus_tag="AC158Q_10050"
     CDS             3368..3841
                     /locus_tag="AC158Q_10050"
                     /inference="COORDINATES: protein motif:HMM:NF024901.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HIR3340159.1"
                     /translation="MMRHSWVLAVLLVLPAPQAAADIWECVDESGNKRFTNVKAEAKG
                     CKLLNISPPNTVTAPPGKPQPKTAARPPDFPRVDNDTQRQRDNERRRILDQELANEQK
                     LLEQARKELAEQESQRLGSERNFQRVLDRLEPYKKKVKLHEDNVANLKRELAGTR"
     gene            3984..5096
                     /gene="glnL"
                     /locus_tag="AC158Q_10055"
     CDS             3984..5096
                     /gene="glnL"
                     /locus_tag="AC158Q_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011325719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HIR3340160.1"
                     /translation="MTPVALSGLDLLATAVVVVDARLAVRYMNPSAENLFELSSRNVA
                     GQKLNEVFSETTVLEAAIDYARTNNCSYTEHDLELDVSGRARLHLACTVTPMEFSGDP
                     RKEELLLEFRHIEQQLRIAREERLLDQSQANRELIRNLAHEIKNPLGGIRGAAQLLDR
                     ELERPNLHEYTQVIMKEADRLQLLMDRLLTPHRLPQPGRLNMHEVLERVRSLILAEYP
                     RDIVIHRDYDVSLPLLKGDREQLIQAVLNIVRNAAQAVVGGGAPGEIRLRTRVARQVT
                     LARRRYRHALAVEVTDNGPGIPPELQERVFHPLVSGRDGGSGLGLTLAQNFVNQHHGM
                     ISFESAPGNTTFMILLPVEDRDEGGGMRAEGRSEKP"
     gene            5224..6621
                     /gene="ntrC"
                     /locus_tag="AC158Q_10060"
     CDS             5224..6621
                     /gene="ntrC"
                     /locus_tag="AC158Q_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010395221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HIR3340161.1"
                     /translation="MKPVWIIDDDRSIRWVFEKALARENIAFKTFSSAQEALAALKTE
                     TPQVVVSDIRMPGESGLELLQRAKALHATLPVIIMTAYSDLESAVTAFQGGAYEYLPK
                     PFDVDHAVELIRRAMEESVRQESVTEETEAAPEILGQAPAMQEVFRAIGRLSQSHATV
                     MITGESGTGKELVARALHRHSPRSAKPFIAINTAASPRELLESELFGHERGAFTGAQA
                     QRRGRFEQAEGGTFFLDEIGDMPQELQTRLLRVLSDGHFYRVGGHQPIKANVRVIAAT
                     HQDLEARVKQGLFRDDLFHRLNVIRLRLPSLRERREDIPLLARHFLAHSARELGVEAK
                     RLSEATLKYLVAQDYPGNVRQLENLCHWLTVMAPSVTIEVKDLPPELRAEVAPAATSW
                     VAALEKEVEMQLGRGESGVMDELTRRFEKALIVKALAHTGGRRIEASQLLGLGRNTLT
                     RKIQELGIDSDKKGQ"
     gene            complement(6763..8067)
                     /locus_tag="AC158Q_10065"
     CDS             complement(6763..8067)
                     /locus_tag="AC158Q_10065"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR3340162.1"
                     /translation="MKTIKVYSALARLAVLCAIAPLAAAADTSEGVFKNALNFTVQVR
                     STLPVPFDGDRKGSGLGAGFLVDAGRGWIMTNAHVVGRSPSRVEVAFHGQEFSEARKV
                     YVDPFLDLAIIRVSESIDTRGIAEPRLECDTIPSVGHGVGAFGHPWRLPFTGTRGIIS
                     GVTARYRTELLQTDAPINQGNSGGPLISLESGRIVGINTAGIRGAQNTNFAVAIKYAC
                     RILQLLRQGQDPSPPDLKLVYFRDVDNRKELKVARSYAADGTLKLLPGDVIRRVSGMD
                     GAIENETQLLHALRGRLGNSSISVLRAGQEVVVAGAKRPMQRVLETRGVYVSGVLFGP
                     ITIRDALEIGARGLMVHHVEPGSIGESREIEKSDLLEAVDGAPVPDVDALHARLNEAR
                     QNGRRVTLMFKKLAGGSTLFSYSERNLLIADLRIIGPQKASD"
     gene            8193..8477
                     /locus_tag="AC158Q_10070"
     CDS             8193..8477
                     /locus_tag="AC158Q_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HIR3340163.1"
                     /translation="MPKAVWNGQVIAESDRCEVVEGNQYFPPEAVKRELFKDSATHTT
                     CGWKGIASYYDVLVDGQVNKDAAWYYPDPKPAAKNIAGYIAFWKGVKVES"
     gene            8645..9100
                     /locus_tag="AC158Q_10075"
     CDS             8645..9100
                     /locus_tag="AC158Q_10075"
                     /inference="COORDINATES: protein motif:HMM:NF024844.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR3340164.1"
                     /translation="MAAEQYFYEDFRPGQRFPGTPHTLDEDAFRLFAQMTGDAHPLHY
                     DADYAKTTRFGAPLAHGLLLMGMTALGAGSLSPHVEDSMIAFLEQGGRFLKPVLLGDT
                     VTPELEVAETRPSKSGPSGIVKLTARLTNQRGETVLEGFHTYLIKRSAT"
BASE COUNT         1748 a         2943 c         2955 g         1607 t
ORIGIN      
        1 aatgaccagt gataagtgac gagtgacaag atctgccggc agcgactgtc caaagcgccc
       61 atcacccgtc acttgccacg ctttttcagg tggtcgcgta gaatggcgcg ctgaatggat
      121 ctcgcgctct atctgaaggt cgtcgttctc ggcatcgtcg aaggcctgac cgaattcctg
      181 ccgatctcca gcaccgggca tctcatcatc gccgggcagt tgctcggctt cggcggcgac
      241 aaggccaagg tcttcgacat cgcgatccag agcggggcga tgctcgcggt ggtgtgggaa
      301 taccgcgcgc gcttcgcccg cgtgctgacg gggctcgcgg gcgatccggt ggcccggcgc
      361 ttcgcggcaa acctggtgat tgcattcctg ccggcggcgc tgctcggcct tgcgttttcg
      421 ggcgcgatca agcagtacct gtttcacgcg gtccccgtcg ccctggcttt catcatcggc
      481 gggctggtca tcctctgggt cgagcgccgg ccgcggcgga cgaccgtgga agaggtggac
      541 gacatgacgt ggaaggacgc gctcaaggtc gggctcgcgc agtcggtggc cctgataccc
      601 ggcacctcgc gctcgggggc gacgatcatc ggcgggatgc tgttcgggct gtcacgccgc
      661 gccgccaccg aattctcatt ttttctcgcg gtgccgaccc tgatcgcggc aggcgcctac
      721 gatctctacc agcatcgcgc gctgctcgac gcggccgacc tgggcccgtt cggcgtcggc
      781 ttcgccgtct cgttcgtctc ggcttttgcc tgcgtgcgct ggctgctgcg ctacatcgca
      841 acccacgact tcagcgtgtt cgcctggtac cgcatcgtgt tcggcgtcat cgtgcttgtc
      901 accgcctaca cgggcttggt gaactggacg gcgtagatgt caatgatgaa tgatgagtga
      961 tgaatgaaaa tccattccac ggggttgggg gctcaattca tcattctgca ttccgcattc
     1021 atcattaacc ctgcgtccag ggcagtcccg ccgcgcgcca gccgccgacg gtattgcgat
     1081 gaccctgcgc gtcgcggtcg ccctcgaatc cctcgagcac gttataggcc gcgctgtaac
     1141 cggcctgcgt cgcaagcatg gccgcgttgt gggagcgccc gccgctgcgg cagatgaaca
     1201 ggaccggggc ggccttgtcc acttggcttt cgagttccgc cacgaacgca ggattgggcc
     1261 ggttcgcggg ataggtgagc agctcgattt ccaccgcgcc ggggatgcgg ccgacgtagt
     1321 cccattcggc gcgggtacgt acgtccacga gcttcgcacc cggcgcgttg cgccacagtt
     1381 cgtgcgcttc ggacggtgtg agcgcgccgc cgtaggaaag gcccatttgc cgcgctcgct
     1441 cggcagcctt ccggagtatg tcgtcgatgg ccatgtcggt ccttgaatgg gggtccgcaa
     1501 tatccatggc gcagccggca ccggcgtcca tggatgtagg cggctggaaa ataagcagta
     1561 ttttacagtg aggccgacgc ctgtacgact gcaccacatc gaggcacaaa gctcataggt
     1621 gcactatact ggtgcgtcac ggggcctatc ggagtaataa accccttgtg gcacaatgcg
     1681 tttcgcgcga aaaaccttgg cacaattcct gctggacaga aggtcccgat ttccctaatt
     1741 cgctttcaat ttcaaatcag gagagctaga tggcaaccgt cgccgacgta ctgaaaatgg
     1801 tcaaggacaa cgaggtgaaa ttcgtcgatt tccgctttac cgacacccgt ggcaaggaac
     1861 agcacgtttc ggtgccggta tcggcgttcg gcgaagccaa attcaccgac ggccacttct
     1921 tcgacggttc ctcgatcgcc ggctggaagg gcatcgaagc gtccgacatg acgctcatgc
     1981 ccgatcccga caccgcgcgc atggacccgt tcaccgacga gccgacgctg ctcctgacct
     2041 gcgacgtgat cgagccgtcc gacggcaagg gctacgaccg cgacccgcgc tcgatcgcca
     2101 agcgcgggga ggcctacctg aaatccaccg gtattgccga caccgcgttc ttcggtcccg
     2161 agcccgagtt cttcatcttc gattcggtga cctggaaggt cgagatgggc caggcgttct
     2221 acaagatcag gtccgaggaa gcgccgtggt ccaccggcga agaattcgag ggcggcaaca
     2281 tggggcaccg cccgccggtg aagggcggct atttcccggt gccgccgctc gattcgctgc
     2341 aggacatccg ctcggcgatg gtgctcgcca tccaggagat gggcgtgccg tgcgaagtgc
     2401 atcaccatga agtggccgcg gcggggcagt gcgaaatcgg cacgctgttc gccccactgg
     2461 tgaagcgcgc cgactggctg atgatcctca agtatgccgt gcataacgtg gcgcactcct
     2521 acggcaagac cgcgaccttc atgccgaagc cggtagtggg tgacaacggc tcgggcatgc
     2581 acgtgcacca gtcgctggcg aagggcgggc agaacctctt ctccggcaac ggctacgcgg
     2641 ggctctcgga actggcgctg cactacatcg gcggcatcct caagcacgcc aaggcattga
     2701 acgcgatcac caaccccggg accaattcct acaagcgcct ggtgccggga ttcgaggcgc
     2761 cgatcaacct ggcgtactcc tcgcgcaacc gttccgcgtc ggtgcgggtg ccgctggtgt
     2821 caaacccgaa agcgcgccgc atcgaagtgc gcttccccga cccgaccacc aacccgtacc
     2881 tcgcattcac cgcgattatg atggccggtc tcgacggcat ccagaacaag atcaagcccg
     2941 gcgacccgat cgacaagaac ctctacgacc tgccgccgga aatcgccgcg aaggtgccga
     3001 aggtgtgcca ctcgctcgag caggcgctgg aggaactcga gaaggatcac gagttcctgc
     3061 tgcgcggcgg ggtgttctcg agggacatgc tggacgcgta cgtggcgctg aagcacgaag
     3121 agctcaccgc cttccgcatg accacccacc cgctggaatt ccagatgtac tacagcctgt
     3181 aaatccgggc cgcggccgat gctcaaaggg cgggaaattc ccgccctttt tgtcgcccgg
     3241 gcttttcgca ccgtttgggt gcgttgccaa atgtgttttg ttgacataga cttaggcagg
     3301 aatgatgaac cgcgccggtc aacggcgggg gtgggatcgg cgttaccggt atgaggagcc
     3361 ccaaccgatg atgcgtcaca gctgggtact ggccgtgttg ctcgtgcttc ctgccccgca
     3421 ggcggccgcg gacatctggg agtgcgtgga cgagagcggc aacaagcgct tcaccaacgt
     3481 caaggcggag gcgaagggct gcaagctgct caacatttcg ccgcccaaca ccgtaaccgc
     3541 gccgcccggc aagccgcagc ccaagaccgc ggcgcggccg ccggattttc ccagggtgga
     3601 caacgatacc cagcggcagc gcgacaacga acgccgcagg atactcgacc aggaactggc
     3661 caacgagcag aagctgctgg agcaggcgag aaaagaactg gcggagcagg aatcccagcg
     3721 gctcggcagc gagcggaatt tccagcgcgt gctcgaccgg ctcgagcctt acaagaagaa
     3781 ggtcaagctg cacgaagaca acgtcgccaa cctgaagcgc gaactggccg gcacccggta
     3841 gggcggcggc ccagggcccg gtgacatgac cacgcctgcc gggccggact ccggtttctc
     3901 gctaccgtgc ccggcgccga ctcaccaggc ggccgccgcg cttggcgcgg ttcttgctct
     3961 taccccgtac ccggaaccgt gacatgacac ccgtagccct ctccggtctc gacctgctgg
     4021 cgaccgcggt ggtggtggtg gatgcgagac tcgccgtgcg ctatatgaat ccgtcggccg
     4081 aaaacctgtt cgagctgagc agccgaaacg tggcgggcca gaagttgaac gaggtatttt
     4141 ccgagaccac cgtgctcgag gccgcgatcg attacgcgcg caccaacaat tgcagctaca
     4201 ccgagcacga cctcgagctc gacgtgagcg ggcgcgcgcg gctgcacctc gcctgcaccg
     4261 tcaccccgat ggaattctcg ggggacccgc gcaaggagga gctgctgctc gagtttcgcc
     4321 acatcgagca gcagttgcgg atcgcccgcg aggagcgcct gctcgaccag agccaggcca
     4381 accgcgaact gatccgcaat ctcgcgcacg agatcaagaa cccgctcggc ggcatccgcg
     4441 gggcggcgca gctcctcgac cgcgagctcg aacgccccaa cctgcacgag tacacgcagg
     4501 tgatcatgaa ggaggccgac cgcctgcaat tgctgatgga ccggctgctg acgccgcacc
     4561 gcctgccgca gccgggccgg ctcaacatgc acgaggtgct ggaacgggtg aggagcctca
     4621 tactcgccga atatccgcgg gacatcgtca tccaccgcga ctacgacgtc agcctgccgc
     4681 tgctgaaagg cgaccgggag cagctgatcc aggcggtgct caacatcgtg cgcaacgcgg
     4741 cgcaggcagt cgttggcggc ggtgcgcccg gggaaatcag gctgcgcacc cgcgtcgcgc
     4801 ggcaggtgac gctggcgcgc cgccgctacc gccatgcgct cgccgtggaa gtcaccgaca
     4861 atggccccgg cataccgccg gaactccagg agcgcgtctt ccatccgctg gtttcaggcc
     4921 gcgacggcgg cagcggcctg gggctcacgc tcgcgcagaa cttcgtcaac cagcaccacg
     4981 gcatgatttc cttcgaaagc gcgcccggca ataccacttt catgatcctg ctgcccgtcg
     5041 aagacaggga tgagggcgga gggatgaggg cggagggacg aagcgagaaa ccctaatgaa
     5101 acaggcccaa tctcttgatt ctttcggagg attattcgat ctcagcgaac tgctgtggtc
     5161 gtgtgagcgt ttacttacgc gcaattgtcc tcatccctca tccctcatcc ctcatccctc
     5221 gacatgaagc cggtctggat catcgacgac gaccgctcga tccgctgggt attcgagaag
     5281 gcgctcgcgc gcgagaacat cgcgttcaag accttctcct cggcgcagga agcgctggca
     5341 gcgctcaaga ccgagacgcc gcaggtggtg gtgagcgata tccgcatgcc gggcgaatcc
     5401 ggcctcgagc tgctgcagcg ggcgaaggcg ctgcacgcca cccttccggt gatcatcatg
     5461 accgcctact cggaccttga gagcgcggtc accgcgttcc agggcggtgc ctacgagtac
     5521 ctgcccaagc cgttcgacgt ggaccacgcc gtggagctga ttcggcgcgc aatggaagag
     5581 agcgtgcgcc aggagagcgt gaccgaggag acggaagccg cccccgagat actgggccag
     5641 gcgccggcga tgcaggaagt gttccgcgcc atcgggcggc tgtcgcagtc gcacgcgacg
     5701 gtcatgatca ccggcgagtc cggcaccggc aaggagctgg tggcgcgcgc gctgcaccgc
     5761 cacagcccgc gctcggcgaa gccttttatc gccatcaata ccgcggccag tccccgcgag
     5821 ctgctggagt ccgagctgtt cggccatgag cgcggcgctt tcaccggggc gcaggcgcag
     5881 cggcgcgggc gcttcgagca ggcggaaggc ggcaccttct tcctggacga gatcggcgac
     5941 atgccgcagg agctgcagac gcgcctgctg cgggtgctct cggacgggca cttctatcgc
     6001 gtcggcggcc accagccgat caaggccaac gtgcgagtga tcgcggccac gcaccaggat
     6061 ctcgaagcgc gcgtgaagca gggcctgttc cgggacgatc tcttccaccg gctgaacgtg
     6121 atccggctgc gcctgccgtc attgcgcgag cggcgcgagg acatcccgct gctcgcgcgc
     6181 cactttctcg cgcacagcgc gcgcgaactg ggggtcgagg cgaagcggct gtccgaggcg
     6241 acgctgaagt atctcgtcgc gcaggactac cccggcaacg ttcgccagct tgagaacctg
     6301 tgtcactggc ttaccgtgat ggcgccttcg gtcacgatcg aggtgaagga cctgccgccc
     6361 gaattgcggg ccgaagtggc gccggccgcg acaagctggg tggcggcgct tgaaaaggaa
     6421 gtcgagatgc aactcggccg cggcgagtcc ggcgtcatgg acgagctcac ccgccggttc
     6481 gagaaggcgc tcatcgtgaa ggcgctcgcc cacaccggcg ggcgccggat cgaggcttcg
     6541 caactgctcg gcctcggccg caacacgctc acgcgcaaga ttcaggaact ggggattgat
     6601 tccgacaaga agggccagtg actggaaccg gtctcgaaat ttgccgcgga atcacacgaa
     6661 tgacacggac gataacccga ctgccttatt ccccgtaatc attcaagaat tcccgagtgt
     6721 ttccctgtgt ttccgtggtt aaaacggctc ttgagatgac ttctagtcac tggctttttg
     6781 ggggccgata atccgcaggt cggcgatcag caggttgcgc tcggagtagg agaacagcgt
     6841 gctgcccccc gcgagtttct tgaacatcag cgtgacccgg cgcccgttct ggcgcgcctc
     6901 gttcaggcgg gcatggagcg cgtccacgtc gggcaccggc gcgccgtcca ccgcttccag
     6961 caggtcggat ttctcgattt cccgggactc cccgatcgag ccaggctcga cgtggtgcac
     7021 catcaggccg cgagcgccga tctccagcgc atcgcggatc gtgatcggcc cgaacagaac
     7081 gccggaaacg tacacgccgc gggtttcaag aacacgttgc atcgggcgct tggcgccggc
     7141 aaccacgact tcctgtcccg cgcgcagcac gctgatgctc gagttgccga gcctgccgcg
     7201 aagcgcgtgc agcaactggg tctcgttctc gatcgcgccg tccattccgg acactcgccg
     7261 gatgacgtcg ccgggcagga gcttcagcgt tccgtcggcc gcgtagctgc gggccacctt
     7321 cagctccttg cggttgtcca cgtcgcggaa atagacgagc ttgagatcgg gcggcgaggg
     7381 atcctgccct tgccgcaaca gttgcaggat gcggcaggcg tacttgatcg ccaccgcgaa
     7441 gttggtgttc tgtgcaccgc ggatgccggc agtgttgatg ccaacgatgc ggccgctttc
     7501 caggctgatc agcgggccgc cggaattgcc ctggttgatc ggcgcgtcgg tctgcaggag
     7561 ttccgtccgg tacctcgcgg tgacgccgga aataatgccg cgcgtgccgg tgaacggcag
     7621 ccgccacggg tggccgaagg cgcccacgcc gtggcctacc gatgggatcg tgtcgcactc
     7681 gagccgcggc tccgcgattc ccctggtgtc gatgctttcg gaaacgcgga tgatcgccag
     7741 gtcgagaaag ggatccacgt agaccttccg cgcctcggag aattcctggc cgtggaacgc
     7801 gacctcgacg cgcgagggcg agcggccgac gacgtgggca ttggtcatga tccagccgcg
     7861 gccggcgtcc accaggaagc ccgcgccgag gccggagccc ttgcggtcgc cgtcgaacgg
     7921 caccggcagc gtggaccgga cctggacggt gaagttgagc gcgttcttga atacgccttc
     7981 gctggtgtcg gcggccgcgg cgagcggcgc gatggcgcag aggaccgcga ggcgcgccag
     8041 ggcggagtaa actttgatcg ttttcatgac gcctatttta ggttaaaccc ggacgctgcg
     8101 cgccgggtta actccacgcg ttcgaacgga cggaatgccc gccgttcaac gcgcggccag
     8161 caacagtggt ctaatacaga tcaggaggtg ctatgccgaa agcggtctgg aatggacaag
     8221 tgattgcgga aagcgaccgg tgcgaggttg tggaaggaaa tcaatatttc cctcctgagg
     8281 cggtaaagcg tgagctattc aaggacagcg caacacacac aacctgcgga tggaagggta
     8341 tcgcgtctta ctatgacgtc ctggtggacg ggcaagtcaa caaggatgcg gcgtggtatt
     8401 accccgatcc caagccggcg gcgaagaaca tcgccggcta catcgctttc tggaagggtg
     8461 tgaaagtcga gtcttaaatt agccgccaag acgccaaggc cgccaagaaa agcacaacaa
     8521 taattgaacc gccaagagcg ccaagcacgc caagaaaaac aagaatggaa ccgcaaataa
     8581 acgcagatta acgtagatac ttacctacaa agtgaaagcc tgaccctttt cgctgggaaa
     8641 atccttggcg gccgaacagt atttctacga agacttccga cccgggcagc gcttccccgg
     8701 gacgccgcac acgctcgacg aggacgcctt ccgtttgttc gcgcagatga ccggcgatgc
     8761 ccatccactg cattacgatg ccgattatgc caagactacg cgttttggtg cgccactcgc
     8821 acacggcttg ctgctgatgg gaatgacagc cttgggcgca ggctcactct ccccgcatgt
     8881 ggaggactcg atgattgcct tcctcgagca gggtggacgt tttctgaagc cggtgctgct
     8941 cggcgataca gtaacaccag agctagaggt cgccgagacg cggccttcga aaagcgggcc
     9001 cagcggaatt gtgaagctta ccgcgcgtct taccaaccaa cgcggcgaaa cggtcctcga
     9061 agggttccac acctacctca ttaaacggag cgccacgtaa cgctggaggc ccacggtgta
     9121 gcttgaagtt cagcccctgt gattcagtgt cgcgacgtaa tacgtcggca tatttatggt
     9181 ggtaaatggc gcacttatcg cccttcgtgg cgcggccgcc gcttggagcg acgaccgcgc
     9241 tacgccggaa cgg
//
LOCUS       DBNGSG010000390         4362 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1314121, whole genome shotgun sequence.
ACCESSION   DBNGSG010000390 DBNGSG010000000
VERSION     DBNGSG010000390.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4362)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4362)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4362
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1314121"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="AC158Q_10080"
     CDS             <1..608
                     /locus_tag="AC158Q_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013390873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340165.1"
                     /translation="TLGHPGKYTYCIAENEEASPFAPYHVEKGYPAQDSTVFVLAAEP
                     PHSITNHYANDAEGILDSVCSAMSTICNNNAVSGGHCAVVLGPEHAAAIAKSGFKRHD
                     IRHYLWAHSGNRWGDLYGNGRYGKVYNRNLPVWYKREPDARTPIVPSPDNIHLFVAGG
                     EAGRFSAFIPGWGHMSSPVLRGVGAAQPAGGPECSDGTCSV"
     gene            832..1377
                     /locus_tag="AC158Q_10085"
     CDS             832..1377
                     /locus_tag="AC158Q_10085"
                     /inference="COORDINATES: protein motif:HMM:NF049802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1120..1122,aa:Sec)
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HIR3340166.1"
                     /translation="MTKTIPVYDPRGVVEAAPLATAPRVKELAGLRLGLLDNTKWNAN
                     KLLRGVRDRLAAKHGFAAVNYYRKESFSLAAAPALLAEIAAGNDVVITAIGDUGSCTS
                     CCMHDTVMLESRGLPTAAIVTGEFLLEAQTQRDALGMSGLDAVVIAHPLSSLTDGEIG
                     LRIEQALPQIERIWRSGSARS"
     gene            complement(1394..2035)
                     /locus_tag="AC158Q_10090"
     CDS             complement(1394..2035)
                     /locus_tag="AC158Q_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HIR3340167.1"
                     /translation="MGVVRFVMAHYDAVLFDLLTALIDSWSLWDRVAGGRDAGRRWRA
                     AYLELTYGCGPYRPYEELVREAALATGLTVHAAERLASGWESLAPWDDAAKTLRRVRR
                     SARIGVVSNCSERLGRQAAALLGVPLDVVVTAERAGFYKPDPAPYRLALAELKLPPAR
                     VLFVAGSGFDLIGTHAVGLPVVWHNRAGIAAPPSAPAPMLERRTLGELFPGPE"
     gene            complement(2069..3352)
                     /locus_tag="AC158Q_10095"
     CDS             complement(2069..3352)
                     /locus_tag="AC158Q_10095"
                     /inference="COORDINATES: protein motif:HMM:NF018513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR3340168.1"
                     /translation="MNITGIVVLVLLVLSVPIGIALSASSAAFILFDPLLQPEVIFRA
                     FFNFISRYSLMAIPLFIFAGFLMEKTGLVGQLFKFSDSLVGWMPGGFGVATVMACVMF
                     GAISGSSVAMAAAMAVIAIPEMLQRGYPPWLAAGLVATAGGIAMLIPPSIILILYGIV
                     TETSVVRLFFAGVSPGLLLAIGNIITVMLIAWLLKLPRGTFDRRRLWAESKGALPAFG
                     MPVVILGGLYGGLFTPTEAAAAACGYALIYGFIRKRGEFFKDLLPVAARSLNLTAVVL
                     FLVGSVGVFQFLMANLYWPQKITEAVVGVGLTPHGFLFAYVGVLLLLGMFVDGVAMVL
                     LTVPVVFPVAQALKIDPVHLAIIVTLAVETGVVTPPVGLNVFAVSGTAKIPVLEVTKG
                     TIPFFVGDLTVLVVVIFFPALATWLPNLLVVDVFK"
     gene            complement(3379..3933)
                     /locus_tag="AC158Q_10100"
     CDS             complement(3379..3933)
                     /locus_tag="AC158Q_10100"
                     /inference="COORDINATES: protein motif:HMM:NF016200.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR3340169.1"
                     /translation="MEKAFAIWCAFQDRILGRVASLILLGCTLLALLEIFRRYVLGFS
                     YEWQQDAVTFFILSGVYLYFSISQRHGEHLAVTVLPEVLEVVGPRARKAAELIKLVAL
                     VLSFLFMLALVWWGIPEVREAVHYETRTESLSFPMWPFLAVLLVGFLFMAVSMFFQIY
                     RGIQKLRGRTVLEEPSEADGLHRQ"
     gene            complement(3959..>4362)
                     /locus_tag="AC158Q_10105"
     CDS             complement(3959..>4362)
                     /locus_tag="AC158Q_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340170.1"
                     /translation="FVGDYYMISASRRWWDRLTPPTRAALEKLIGETIQVQKEYNWCF
                     DKIAYDKYGTKDPSKPGVYWLTPQEVSAMVGAMGDAAINYVKSKTPPAAAKWVDNFVK
                     EGRELSKKHPSGSSWIEKVDCSKHASVIVVK"
BASE COUNT          885 a         1451 c         1380 g          646 t
ORIGIN      
        1 gcacgctggg ccaccccggc aagtacacct actgcatcgc cgagaacgag gaagcgagcc
       61 cgttcgcacc ttaccacgtg gaaaaaggct acccggcgca ggattccacc gttttcgtgc
      121 tggccgccga gccgccgcac agcatcacca accactacgc gaacgacgcg gaaggcatcc
      181 tcgacagcgt ctgctcggcg atgagcacca tctgcaacaa caatgcggtc tcgggcgggc
      241 actgcgcggt ggtcctcggc cccgaacacg ccgccgccat cgcgaaatcg ggcttcaagc
      301 gccacgatat ccgccactat ctgtgggcac actccggcaa ccgctggggc gacctctacg
      361 gcaacggccg ctacggcaag gtctacaacc gcaacctgcc ggtgtggtac aagcgcgaac
      421 ccgacgcgcg cacgccgatc gtgccgagcc cagacaatat ccatctgttc gtcgccggcg
      481 gcgaggccgg gcgcttctcg gcattcatcc caggctgggg acacatgtcc tcaccggtgc
      541 tgcgcggcgt gggtgccgcg caacccgccg gcggaccgga gtgttcggac ggcacttgtt
      601 ccgtctaaga aaaaccaaat tggccacgaa atcacgctga agtacacgga cgagaaggaa
      661 gatgaggcga cgaaacaaac tatgaaattg ttccagattt ctcgttgcat gcatattttt
      721 cgtgcggtca tgcggtcatg ttcctatgct tgattccgtg tgtttccgtg tgattccgtg
      781 gctgatactg tattttcgat cttgagagga aattttcggc gggaggaaac gatgacgaaa
      841 acaataccgg tatacgaccc gcgcggcgta gtggaggccg cgccactggc aaccgccccc
      901 cgcgttaaag agctggcggg gctgcggctt ggcctgctcg acaacaccaa gtggaacgcc
      961 aacaagctgc tgcgtggcgt gcgcgaccgg ctcgccgcca agcacggatt cgccgcggtg
     1021 aactactacc gcaaggagag tttttcgctc gccgccgcgc ccgctctgct cgccgagatc
     1081 gccgccggca acgacgtcgt catcaccgcc atcggcgact gagggtcctg cacgtcgtgc
     1141 tgtatgcacg acacggtgat gctggagtcc cggggcctgc ccacggccgc gatcgtcacg
     1201 ggtgaattcc tgctcgaagc gcaaacgcag cgcgacgcgc tcggcatgtc cgggctcgat
     1261 gcggtcgtga tcgcccaccc cctgagctcg ttaaccgacg gggaaatcgg gctgcgcatc
     1321 gagcaggcgc tgccgcagat cgaacgcatc tggcgctccg gaagcgcgcg atcctgaagc
     1381 ggcccgcgcc gggttactcc ggcccgggaa acagctcgcc cagagtgcgc cgctccagca
     1441 tgggcgctgg cgcggaaggc ggcgcggcga tgccggcgcg gttgtgccac accaccggca
     1501 atccgaccgc gtgcgtgccg atcaggtcga atccggaacc cgccacgaag agcacccgcg
     1561 cgggaggcag cttcaactcg gcgagcgcga gccggtatgg cgcggggtcg ggcttataga
     1621 aacccgcgcg ctccgcagtg acgacaacgt cgagcggcac cccgagcagt gcggcagcct
     1681 gccgccccag gcgctccgag cagttggaaa ccacgccgat ccgggcgctc cggcgcaccc
     1741 gccgcagcgt cttcgccgcg tcatcccagg gcgcgagaga ctcccacccg gaagcaagtc
     1801 gttcggccgc atgcacggtg aggccggtgg cgagcgccgc ctcgcgcacc agttcctcgt
     1861 aaggccggta gggcccgcag ccgtaagtaa gttccagata cgccgcgcgc caccggcggc
     1921 cggcatcgcg accgccggcg acccggtccc acagcgacca cgagtcgatc agcgccgtca
     1981 gcaggtcgaa gagaaccgcg tcgtaatgcg ccatgacgaa gcggacaacc cccatccctc
     2041 tccctgacaa gggagcggcc ccggggggtc acttgaagac gtcgaccacc agcaggttcg
     2101 gcagccaggt cgcgagcgct ggaaagaaaa tcaccaccac cagcacggta agatccccga
     2161 cgaaaaaggg aatggttcct ttagtgacct cgagcaccgg gatcttcgcg gtaccgctca
     2221 ccgcaaaaac attgagcccc accggcggcg tcaccactcc cgtctcgacc gcgagcgtca
     2281 cgatgatggc aagatggacc ggatctattt tcagggcctg ggcgaccggg aacaccaccg
     2341 gcaccgtcag caacaccatg gcgacaccgt cgacgaacat cccgagcagc agcaggacgc
     2401 ccacgtaggc gaacaggaag ccgtgcggcg tgagtcccac gccgaccacc gcctcggtga
     2461 tcttctgcgg ccagtacagg ttggccatca ggaactgaaa gacgcccacg ctgcccacga
     2521 ggaagagcac gaccgcagtc aggttcaggg aacgggcggc gaccggcagc aggtccttga
     2581 agaattcgcc ccgttttctg atgaagccgt agatcagcgc gtaaccgcag gctgcggcag
     2641 cggcttcagt gggggtgaag aggcccccgt acagcccgcc gaggatgacg accggcatgc
     2701 cgaacgcagg cagcgcgccc ttggactcgg cccagagccg gcgccggtcg aaggtgcccc
     2761 gcggcagctt caggagccac gcgatgagca tgacggtgat gatattgccg atggcgagca
     2821 gcaaccccgg actgacgccg gcgaagaaca agcgcaccac ggaggtttcg gtcacgatgc
     2881 cgtacaggat caggatgatg ctgggcggta tcagcatcgc gatgccgccg gccgtcgcca
     2941 cgaggcccgc cgccagccag ggcgggtagc cgcgctgcaa catctcgggg atggcgatga
     3001 ccgccatggc tgccgccatc gccacgctcg agccggagat cgcgccgaac atcacgcacg
     3061 ccatcaccgt agccacgccg aagccgccgg gcatccagcc caccagcgag tcggagaact
     3121 tgaagagctg gccgacgagg ccggtcttct ccatgaggaa tcccgcgaag atgaacagcg
     3181 gaatcgccat cagcgaatag cggctgatga aattgaagaa agcgcggaag atgacctcgg
     3241 gctggagcag cggatcaaac aggatgaaag ccgcgctgga agcgctcaac gcgatgccga
     3301 tcggcacgct caacacgagc agcacgagga ccacgatgcc ggtgatattc atgcgctact
     3361 cctggtcccg ccggcgcttc actggcggtg cagcccgtcc gcctcggatg gctcctcgag
     3421 tacggtgcgt ccgcgcagtt tctggatgcc gcgataaatc tggaaaaaca tcgagaccgc
     3481 catgaataga aagccgacga gcagcaccgc aagaaacggc cacatgggaa acgacaggct
     3541 ctcggtgcgg gtctcgtagt ggacggcctc gcgcacttcc ggtatgcccc accagaccag
     3601 ggccagcatg aacaggaagg acaggaccag cgcgacgagc ttgatgagct cggccgcctt
     3661 gcgcgcgcgc ggcccgacca cctccagcac ctccggcagc accgtcacgg ccaggtgctc
     3721 gccatggcgt tgcgaaatgc tgaagtacag gtagacgccg ctcaggatga agaaggtgac
     3781 ggcatcctgc tgccactcgt acgaaaaacc cagcacgtag cggcggaaga tctcgagcag
     3841 ggcgagcagc gtgcagccga gcaggatgag cgaggctacg cgtcccagga tccggtcctg
     3901 gaacgcgcac cagatcgcga aagccttctc cacgcgccgg attccgccgc gcggcgcctt
     3961 acttgaccac gatcaccgac gcgtgcttcg agcagtcgac cttctctatc cacgacgagc
     4021 cggaggggtg cttcttggac agctcgcggc cctctttcac gaaattgtca acccacttgg
     4081 ccgccgccgg cggggtcttg ctcttgacgt aattgatcgc ggcgtcgccc atcgcgccca
     4141 ccatggcgga gacttcctgc ggggtcaacc agtacacgcc gggcttggag ggatccttgg
     4201 tgccgtactt gtcgtaggcg atcttgtcga agcaccagtt gtattccttc tgcacctgga
     4261 tggtctcgcc gatcagtttc tcgagcgccg cccgggtcgg aggcgtgagg cggtcccacc
     4321 agcggcggct cgcgctgatc atgtagtaat cgccgacgaa gg
//
LOCUS       DBNGSG010000391         8238 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2164275, whole genome shotgun sequence.
ACCESSION   DBNGSG010000391 DBNGSG010000000
VERSION     DBNGSG010000391.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 8238)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8238)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8238
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2164275"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..147
                     /locus_tag="AC158Q_10110"
     CDS             <1..147
                     /locus_tag="AC158Q_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HIR3340171.1"
                     /translation="VPLDLHYKNGRIKLEIGLAKGKKQYDKRQDEKEKDWRREQQRLM
                     RTKH"
     gene            complement(149..1006)
                     /locus_tag="AC158Q_10115"
     CDS             complement(149..1006)
                     /locus_tag="AC158Q_10115"
                     /inference="COORDINATES: protein motif:HMM:NF013324.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR3340172.1"
                     /translation="MGLEIVVIALLIFAVVTVWKGVRIVPQGEEWIVERLGKFNKTLT
                     PGLTIIVPYIDKVAYKVVTKDIILDVPEQEVITKDNAVIITNAIAFIKVTDPIKAVYG
                     IVDFAEAIRNLVQTTLRSITGEMELDQALSSREKIKLRLKESIADEAVDWGLTIKSVE
                     IQDIKPSESMVKSMELQAAAERERKAVVTKAEGAKQAMILEAEGRLESAKRDAVAQVT
                     LAESSALAIQKVATAMVDRDTPMLYLLGEKYILALDRLAASQNSKFVLLPADLQDALK
                     GILGKSRAA"
     gene            complement(1019..1465)
                     /locus_tag="AC158Q_10120"
     CDS             complement(1019..1465)
                     /locus_tag="AC158Q_10120"
                     /inference="COORDINATES: protein motif:HMM:NF014062.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR3340173.1"
                     /translation="MMQFLWWHWVVLGIVLLLLELAVPAFFLVWFGLGALIVGVVLLA
                     IPSLSFAWQILIWIVFSLAFIWMWFKVFKPGFYKTRAGMSKGAVIGEIGLVIRDIRPF
                     EKGQIRFQKPMLGDEVWESIADEEIKAGERVKVLDVEGNILKVVRT"
     gene            complement(1534..1608)
                     /locus_tag="AC158Q_10125"
     tRNA            complement(1534..1608)
                     /locus_tag="AC158Q_10125"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1574..1576),aa:His,seq:gtg)
     gene            complement(1724..1799)
                     /locus_tag="AC158Q_10130"
     tRNA            complement(1724..1799)
                     /locus_tag="AC158Q_10130"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1764..1766),aa:Arg,seq:tct)
     gene            2007..2429
                     /gene="dksA"
                     /locus_tag="AC158Q_10135"
     CDS             2007..2429
                     /gene="dksA"
                     /locus_tag="AC158Q_10135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HIR3340174.1"
                     /translation="MASELHKPFPAYKPKGKEDYMNSRQLAHFRKMLEEMKRDLSQDI
                     DRTVHTMQDEATIFADPNDRASQESDMSLELRNRDRERKLIKKIDETIARIDAGEYGY
                     CDSCGVEIGLKRLEARPTATLCIDCKTLDELRERQVAK"
     gene            complement(2558..4675)
                     /gene="pnp"
                     /locus_tag="AC158Q_10140"
     CDS             complement(2558..4675)
                     /gene="pnp"
                     /locus_tag="AC158Q_10140"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HIR3340175.1"
                     /translation="MNHIKKSLIWGQHRLVLETGEIARQASGAVLVNMEDTVVLVTVV
                     GAKQAQPGKDFLPLTVDYQERTYAAGKIPGGFFRREGRPSEKEILTCRLIDRPIRPLF
                     PDGFYNEVQIVATVVSSNNEVDSDIPAMIGASAALALSGIPFNGPIGAARVGYLNGQY
                     VLNPTATELKTSQLDLVVAGTGQAVLMVESEAKELPEDVMLGAVVFGHQQMQQVIQVI
                     NELVDEAGSEAWNWKEPAKDEALIERITGLAENDLRAAFQLKQKGARSEAIDNIWKRV
                     FTEVGVGTEGGPDANAVKEICFSLESKIVRGQILSGEPRIDGRNTRTVRPITIRTGLL
                     PRAHGSALFTRGETQALVTATLGTSRDEQIIDALQGGYRDRFMMHYNMPPYATGETGR
                     VGSPKRREIGHGRLAKRALIGMLPGPEAFAYSLRVVSEITESNGSSSMASVCGGCLAL
                     MDAGVPLKAHVAGIAMGLIKEGNRFAVLSDILGDEDHLGDMDFKVAGTETGITALQMD
                     IKIEGITKEIMHAALIQAREGRLHILDKMKHSLESPRSELSMWAPRMITLKIKPEKIR
                     DVIGKGGAVIRALTEETGTTIDIQDDGTVTIACVSPEAGELARKRIEDITADVEVGRI
                     YDGTVLKLLDFGAIVSVLPGKDGLLHISQIANERVNAVSDHLKEGQQVRVKVLEADDK
                     GRLRLSMKAVAGEAKTPTPQTAQ"
     gene            complement(4819..5088)
                     /gene="rpsO"
                     /locus_tag="AC158Q_10145"
     CDS             complement(4819..5088)
                     /gene="rpsO"
                     /locus_tag="AC158Q_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HIR3340176.1"
                     /translation="MAVTNNQKAQLVKDYQRAKDDTGSPEVQVALLTARITDLTEHFK
                     THVKDHHSRRGLLRMVSRRRKLLDYLKRADADKYRQLIDRLGLRK"
     gene            complement(5231..6142)
                     /gene="truB"
                     /locus_tag="AC158Q_10150"
     CDS             complement(5231..6142)
                     /gene="truB"
                     /locus_tag="AC158Q_10150"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007000134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HIR3340177.1"
                     /translation="MSDVRTGRRIVDGVLLLDKPSGITSHTAMQKVRRLFNAAKAGHT
                     GTLDPLATGLLPVCLGEATKFSHLLLEADKTYLAVIRLDLTTRTGDLDGEVTASFPVI
                     NVKRERVDEVLRDFAGETHQTPPMYSAIKHAGQPLYKLARAGRDIPRHPRKIFISSMI
                     MVDMHGNELTVRVCCSKGTYVRVLAEDIGRKLGCGGCLAALRREVVGEFALTSGAVTL
                     EQLEALTPAQRGELLMPPDALVKSLPRLDLDAGDTRRLSHGQTVERTAASAEGLARIY
                     GPDREFLGIAEITGPGRIIPRRLTARG"
     gene            complement(6139..6534)
                     /gene="rbfA"
                     /locus_tag="AC158Q_10155"
     CDS             complement(6139..6534)
                     /gene="rbfA"
                     /locus_tag="AC158Q_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HIR3340178.1"
                     /translation="MPRDYPRSRRIAEQIQRELSDIIRLELKDPRVGMITIIDVEVTQ
                     DQSHAKVFFTSLGDSSRIADASKGLQNAAGFLRSQLAHRMKLRIVPQLQFKYDVSVER
                     GIHLSQLIDAAVADDAAKAAHPARVRKKK"
     gene            complement(6623..>8238)
                     /gene="infB"
                     /locus_tag="AC158Q_10160"
     CDS             complement(6623..>8238)
                     /gene="infB"
                     /locus_tag="AC158Q_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIR3340179.1"
                     /translation="IVIEEMGHVAKRAKLDEPDAFLAEEQQAPSAIKLEPRAPVVTVM
                     GHVDHGKTSLLDAIRRTKVASGEAGGITQHIGAYHVNTPKGMVTFLDTPGHEAFTAMR
                     ARGAKVTDIVILVVAADDGVMPQTIEAIHHARAAKVPMVVALNKIDKPEANPDRVKQD
                     LSAQQVVPEEWGGDTMFIPVSAKTGKGVDQLLDSLLVQSEVLELRAAKDAPAKGIVIE
                     SRLDKGRGPVASVLVQSGTLKRGDVVLAGAVFGRVRAMLDENGESIEQAGPSIPVEIL
                     GLSDVPMAGADVLALGDERKAREIALFRQGKYRDVKLARQQSAKLENIFDQMGEGQAK
                     SLSLIIKADVQGSYEGLSHALSGLSTNEVKVNIVHAAVGGITESDVNLALASKAVIIG
                     FNARADAAARKLAENSGIDIRYYNVIYDAVDEIKSALSGMLSPERKESVLGLVEVRQI
                     FRISKIGTVAGCYVTEGLVRRSGAKVRVLRDNVVIHDGELDSLKRFKDDVREVKSGFE
                     CGLSLKNFDDLKVGDQLEVYEVVEVARTL"
BASE COUNT         1637 a         2618 c         2313 g         1670 t
ORIGIN      
        1 gtgccgctcg acctgcacta caagaacggg cgcatcaagc tcgagatcgg gcttgccaag
       61 ggcaagaaac aatacgacaa gcggcaggac gaaaaggaga aggattggcg ccgcgagcag
      121 caacgactaa tgcggaccaa gcactgattt aggccgcccg gctcttcccg agtatccctt
      181 tcagcgcgtc ctgaaggtca gccggcagca gcacgaactt gctgttctgc gaggcggcaa
      241 gcctgtcgag cgcgaggatg tacttctctc cgagcaggta cagcatgggc gtatcgcgat
      301 ccaccattgc ggtcgcgact ttctgtattg ccagcgcgga agattctgcc agcgtcactt
      361 gagccacggc gtcccgcttg gcgctttcca gccgcccctc ggcttcgagg atcatcgcct
      421 gcttcgcgcc ttccgccttg gtcaccacgg ccttccgttc tcgctcggca gcggcctgca
      481 gttccatgga tttgaccatc gactcggaag gcttgatgtc ctgaatctcg acggacttga
      541 tggtaagacc ccaatcaacc gcctcgtcgg cgatgctctc ttttagccgc agcttgattt
      601 tctcccgcga agacaatgcc tggtcgagct ccatttcacc cgtaatcgag cgcagcgtgg
      661 tctgaaccag gttgcggatc gcctcggcaa aatccacgat tccgtaaacg gccttgatcg
      721 gatcggtcac cttgatgaag gcgatcgcat tggtgatgat cacggcattg tccttggtaa
      781 tcacctcctg ctccgggaca tcgagaatga tgtccttggt gacgaccttg taggcgacct
      841 tgtcgatgta gggaacgatg atcgtcagtc cgggggtcag cgtcttgttg aactttccga
      901 ggcgctcgac gatccactcc tcgccctgag gaacgattct cacgcccttc cagacggtaa
      961 caaccgcaaa aatcagcaat gcaatgacga cgatttccaa tcccatgacg cactccccct
     1021 aggttcggac aaccttcagt atattgccct cgacgtcgag caccttcacc ctttctccgg
     1081 ccttgatttc ctcgtcggcg atcgattccc acacctcatc gcccagcatc ggcttctgga
     1141 agcgaatctg ccctttttcg aagggacgaa tatcccggat aaccagcccg atctctccga
     1201 taaccgcgcc cttggacatg cccgcgcggg tcttgtaaaa acccggcttg aacaccttga
     1261 accacatcca gatgaaggcg agggaaaaga cgatccagat caggatctgc caggcgaatg
     1321 acagggatgg aatggcgagc aggacaacgc cgacaataag cgcccccaag ccgaaccaca
     1381 ccaggaagaa tgccggcacc gcgagctcga gcagcagcag cacgataccc agcacgaccc
     1441 aatgccacca caagaactgc atcattgcgc cccccgtaaa aaccggcgac cgcagcgatt
     1501 agagcacgat cagagagtca cggcaaagca agatggggtg gctgatggga ctcgaaccca
     1561 caacacctgg aatcacaatc cagtactcta cctttgagct acagccacca tataaaccga
     1621 atgcaaggtg caaagtgcag aatgcaaagc gcaatttcac gagcgcccgc aaacgccgat
     1681 cagccaattc cgcaatttgc actttgcatt ttgcactgaa tattggcacg cccggcggga
     1741 atcgaaccca caacccccgg cttagaaggc cggtgctctg tccgttgagc tacgggcgct
     1801 catcgacaag tctgccgcgt tcgatcgtgg gccgcaaagt gacacatgca acccgggcaa
     1861 ggccgtgccc cgaaagcggc gtaactatac tcttggaacc ctatgatagt caatgagaaa
     1921 tcagagtttt cccgacgctt attcgtcact tgcggggtgc tgggatttca tatataaaga
     1981 gcctcttttt tcgggcatat ccgctcatgg cttccgaact gcataagccc tttcccgcct
     2041 acaaacccaa gggaaaagag gactacatga actcgcgcca gctcgcgcat ttccgcaaga
     2101 tgctggagga aatgaagcgc gacctgtcgc aggacatcga ccgtaccgtg cacaccatgc
     2161 aggacgaggc aacgatcttc gcggacccca acgaccgggc cagccaggaa tcggacatgt
     2221 cgctggaatt gagaaaccgc gaccgcgagc gcaaactcat caagaaaatc gacgagacca
     2281 tcgctcgcat cgacgcgggc gagtacggtt actgcgattc ctgcggcgtg gaaatcggcc
     2341 tgaagcgcct cgaggcgcgg ccgacggcaa cgctatgcat cgactgcaag acacttgacg
     2401 aattgcgcga gcggcaagtg gccaaataag cgttgaagcc ggccgcgggg cgccggttta
     2461 acttcgcgcg ttcaggcgga ggaagcaagt cccccgctca acgcgcgaga aataaaaaag
     2521 cggcctatcg gccgcttttt ttcgcgccgg ctcgatgcta ttgcgcagtc tggggcgtcg
     2581 gggtcttggc ctcgccagcc accgccttca tggaaagccg cagccttccc ttgtcgtcgg
     2641 cctccagcac cttcacccgc acctgctgcc cttccttgag atggtccgat accgcgttga
     2701 cgcgctcgtt ggcgatctgc gagatatgga gcaggccgtc cttgccgggc agcacgctta
     2761 cgatcgcccc gaaatcgagc agcttaagca ccgtgccgtc gtagatacgc ccgacttcca
     2821 cgtcggcggt aatgtcctcg atgcgctttc tggccagttc tcccgcctcc ggtgaaacac
     2881 aagcgatggt gacggtgccg tcgtcctgga tatcgatggt ggtgccggtc tcctcggtca
     2941 gcgcgcgaat gaccgccccg cccttgccga tcacgtcgcg gatcttctcc ggcttgatct
     3001 tgagggtgat catccgcggc gcccacatgg aaagctcgga gcgcggcgat tccaaagagt
     3061 gcttcatctt gtccaggata tgcaggcggc cttcgcgcgc ctggatcagc gccgcatgca
     3121 tgatttcctt tgttatacct tcgatcttga tatccatctg aagcgcggta attcccgtct
     3181 cggtgccggc gaccttgaag tccatgtcgc ccaggtggtc ctcgtcgccg agtatgtcgg
     3241 acagcaccgc aaaccggttg ccttccttga tgagccccat ggctatgccc gccacgtggg
     3301 ccttgagcgg aacgccggcg tccatcagcg ccagacagcc gccgcatacg ctcgccattg
     3361 agctcgagcc gttggactcg gtgatctccg atacgacgcg taacgaataa gcgaacgcct
     3421 cggggccggg aagcatgccg atcagcgcgc gcttggcgag ccgcccgtgc cctatctcac
     3481 gacgcttggg cgacccgacg cggccggttt ccccggtcgc ataaggcggc atgttgtaat
     3541 gcatcatgaa gcgatcgcgg tatccgccct gcagcgcatc gatgatctgc tcgtcgcgcg
     3601 aagtgccgag ggtcgcggtg accagggcct gcgtttcacc ccgcgtaaac agggccgaac
     3661 cgtgggctcg cggcagcaaa ccggtgcgaa tagtgatggg gcgcaccgtg cgggtgttgc
     3721 gtccatcgat acgcggttcg ccgctgagaa tttgcccgcg tacgatcttc gactccagcg
     3781 agaagcagat ttccttgacc gcgttggcat ccgggccgcc ctcggtgccg accccaacct
     3841 cggtaaacac ccgcttccag atgttgtcta tggcctcgga acgtgccccc ttctgcttga
     3901 gctggaacgc cgcgcgcaaa tcattctcag caaggccagt tatgcgctca atcagcgcct
     3961 cgtccttggc tggttctttc cagttccatg cttccgaacc cgcctcgtcc accagctcat
     4021 tgatcacttg gatcacctgc tgcatctgct ggtggccgaa taccaccgcg cccagcatca
     4081 cgtcctcggg caactctttt gcttcggact ccaccatcag cactgcctgc ccggtaccgg
     4141 ccaccaccag gtcaagttgc gaagtcttga gttcagttgc ggtcgggtta agcacgtatt
     4201 gcccgttgag ataacccacg cgagcggccc caatcgggcc attgaacgga atacctgaca
     4261 gggccagcgc ggccgaagcg ccgatcatgg cgggaatatc ggagtcgacc tcgttgtttg
     4321 atgacaccac ggtcgccacg atctgcactt cgttgtagaa gccgtcggga aacagcgggc
     4381 gaatcggccg gtcgatcagc cggcaggtga ggatttcctt ctcggaaggc cggccctcgc
     4441 gcctgaaaaa tccgccggga atctttccgg cagcgtaggt gcgttcctgg taatcaacgg
     4501 tcagaggcag gaaatccttg cccggttgcg cctgctttgc accgaccacc gtcaccagca
     4561 ccacggtgtc ttccatattg accagcactg caccggatgc ctgacgcgcg atctctccgg
     4621 tttccaggac gagacgatgc tgcccccaga taagtgattt ctttatatga ttcaaagcgt
     4681 tgccccttga aaaagaaaaa gaccgcggtg cgcggtctcg gatttaaaga tgcaattcca
     4741 gcggccgcgc ctggaagccc gcgcgacccg gtgtaacgac cagccggccc ctgtcttcgg
     4801 cgtccactac cagatgcctt acttgcgcag cccgaggcgg tcgatcagct gacggtactt
     4861 atcggcgtca gctctcttga ggtaatccag gagcttgcgc ctgcggctca ccatgcgcag
     4921 caggccgcgc cgcgaatgat ggtccttcac gtgcgtcttg aaatgctcgg tgaggtccgt
     4981 gatgcgcgcc gtcagcaacg cgacttgcac ctcgggcgat cccgtgtcgt ccttggcacg
     5041 ctggtaatcc ttaaccagct gagctttctg gttgttggtg actgccattg tcttctccga
     5101 ctgtcctgtt tttccgtctg taagtggcgc gctattctac tctctaaccc acggtttctc
     5161 aagcaattta ggggccgatc agtcgctccg ggccctggta aaccggttgc tcggcattcc
     5221 ggctcctgcc ctagcctctg gcggtaagcc gcctgggtat gattcgaccc ggaccggtga
     5281 tctccgctat ccccaggaat tcccggtcgg gaccataaat gcgcgccagc ccctcggcac
     5341 tggccgcggt gcgctcgacc gtctggccat ggctcaatct ccgcgtgtcg ccagcatcga
     5401 gatcgaggcg aggcagagat ttcaccagcg catcgggcgg catcagcaac tcgcccctct
     5461 gagctggcgt cagcgcttcg agctgttcca aggtaaccgc ccccgaagtc aatgcaaatt
     5521 cgccaaccac ctccctgcgc aacgcagcga gacagccgcc gcaacccagc ttgcgaccga
     5581 tgtcctccgc cagcacccgc acataggtgc ccttactgca acaaacccgc acggtcagtt
     5641 cattcccatg catgtcgacc attatcatgc tgctgataaa tatctttctc ggatgacgag
     5701 gaatgtcgcg cccggcacgc gcgagcttgt aaagcggctg gccggcatgc ttgatagcgc
     5761 tgtacatcgg cggagtctga tgagtttcgc cggcaaagtc cctcagcacc tcgtcgaccc
     5821 tctcacgctt cacattgatg accggaaacg aggctgtgac ctcgccgtcg agatcacccg
     5881 tacgggtcgt aagatcaagc cggataacgg cgagataggt cttgtcggcc tccagcagga
     5941 ggtgggaaaa cttggtagcc tcgcccaggc agaccggcag caagcccgtt gcaagggggt
     6001 cgagtgtccc cgtgtgcccc gccttggccg cgttgaatag gcgtcgaacc ttttgcattg
     6061 ccgtatgcga ggtgatgccg gatggcttgt ccagcagcag cacgccatcc acgatgcggc
     6121 gcccggtacg cacgtcactc attttttctt ccggacacgg gccggatggg ctgcctttgc
     6181 agcatcgtca gccaccgcag catctatcaa ttgtgaaagg tgtattccgc gctcgacgga
     6241 tacatcgtac ttgaactgca attgcggcac gatgcgcagt ttcatgcggt gtgccagctg
     6301 gctgcgcaaa aaccccgcgg cattctgcaa acccttggaa gcatcggcaa tcctggacga
     6361 gtcaccgagc gaggtgaaga acaccttggc gtgggactga tcctgggtaa cctcgacgtc
     6421 gatgatggta atcatgccga cgcgcggatc cttcagttca agtcggatga tatcggacag
     6481 ctcacgctgg atctgctcgg cgatgcggcg gctgcgggga tagtctctgg gcatcaggat
     6541 ccgggattca ggagtcggga ttcaggaaac ccgggcgagc ctgaaagcga actccccgaa
     6601 tcctgaatcc ggaacccgaa tcctagaggg ttcttgctac ttcgaccacc tcgtaaacct
     6661 cgagctggtc gccgaccttg agatcgtcga agttcttgag agacaggccg cactcaaatc
     6721 cggatttgac ctctctcacg tcgtccttga agcgcttgag cgaatcgagc tcgccgtcgt
     6781 gaatcacaac attgtcgcgc agcacgcgca ctttggcccc gctacgccgc accagtcctt
     6841 ccgtcacgta gcagccggcg acggtgccga tcttggaaat cctgaagatc tggcgaacct
     6901 ccaccaggcc gagaacactt tccttgcgct ccggagaaag catgcccgag agcgctgatt
     6961 tgatttcgtc cacggcatcg taaatcacat tgtagtaacg aatgtcgatg ccgctgttct
     7021 cggcgagttt gcgggctgca gcgtcggccc gcgcattgaa gccgatgatg accgccttgg
     7081 aagcgagcgc gaggttgacg tccgactcgg taatgccgcc caccgcggca tgaacgatat
     7141 tgaccttgac ctcgttggtg gagagtccgg acagggcgtg cgacaggcct tcgtaagaac
     7201 cctgcacgtc cgccttgatg atgagagaga gcgatttggc ctgcccttcc cccatctgat
     7261 caaaaatatt ctcgagcttt gccgattgct gcctggccag cttcacgtcg cggtatttcc
     7321 cctggcggaa caacgcgatc tcgcgcgcct tgcgttcgtc gcccagagcc agaacatcgg
     7381 ctcccgccat cgggacgtcg gacaatccga ggatttccac cgggatcgat ggaccggcct
     7441 gctcgatgct ctcgccattc tcatccagca tcgcacgtac ccgtccgaaa acggcgcctg
     7501 ccagcaccac gtctccgcgc ttcagcgtgc ccgattgcac gagcacgctg gcaaccggtc
     7561 cgcgtccctt atcgagccgc gactcaatga cgataccctt ggctggcgcg tccttcgccg
     7621 ccctcaattc gagcacttcg gactgcacca gcaggctgtc gagcagctgg tccacaccct
     7681 tgccggtctt ggccgacacc gggatgaaca tggtgtcacc tccccactcc tcgggtacca
     7741 cttgctgggc agaaaggtcc tgcttgaccc ggtccgggtt ggcctccggt ttgtcgatct
     7801 tgttcagcgc gaccaccatc ggcaccttgg ccgccctggc gtgatggatc gcttcgattg
     7861 tctgtggcat cacgccatcg tcggcagcaa cgaccagaat tacgatgtcg gtcaccttcg
     7921 cgccgcgcgc gcgcatcgcg gtgaatgctt cgtggcctgg cgtgtcgagg aacgtaacca
     7981 tgcccttggg cgtattcacg tggtacgcgc cgatatgctg cgtgatgccg ccagcttcgc
     8041 cgcttgcgac cttggtgcgg cgtatagcat cgagcaggga ggtcttgccg tgatccacgt
     8101 gccccatgac cgtaaccacc ggggcgcgcg gctcgagctt gattgcggag ggtgcctgct
     8161 gctcctccgc gagaaaggca tccggctcgt cgagcttggc gcgcttggca acgtgcccca
     8221 tctcctcgat gacgatca
//
LOCUS       DBNGSG010000392         5466 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3352321, whole genome shotgun sequence.
ACCESSION   DBNGSG010000392 DBNGSG010000000
VERSION     DBNGSG010000392.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5466)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3352321"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..796)
                     /locus_tag="AC158Q_10165"
                     /pseudo
     CDS             complement(128..796)
                     /locus_tag="AC158Q_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008646504.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
     gene            complement(948..2357)
                     /locus_tag="AC158Q_10170"
     CDS             complement(948..2357)
                     /locus_tag="AC158Q_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HIR3340180.1"
                     /translation="MPIHEIGSVEQPLLRTQLLTWLLAPLFLLLTADTFISYWVALNF
                     SQRAYDRTLVEIAREVSLYLKRADGGALAREMPAEARRLLLTDPVDRVYFQVEDAGGR
                     VVAGFPIAPARRDAARRRPALYDGEAEGEAVRVVELALDADGATGRPAAVVRAAETMV
                     KRNVLAREILLSVVIPQVLLILIAGVVVWAGVVRGLSPLQRLQRAVASRSHRDCSPVV
                     VDNVPGEVSPLLHSINELLARLDRVLTLQSRFISDAAHQLKTPITALNAQFELAQREA
                     DPQRMRAAMQALSPALERLSRLVSQLLSLARNEPEAVRAVTLAQLDLNALSLEAATGW
                     VPESRQRSIDLGFEGSAEPVMVRGDAARLRELLDNLLDNAIRYSPAGGRVTVRVSAKP
                     VPTVAVNDDGPGIPAHERERVFERFHRLLGNSADGSGLGLAIAQEIARLHGADIALSD
                     DTDGVGTTFSVRFPAPPPA"
     gene            complement(2669..3346)
                     /locus_tag="AC158Q_10175"
     CDS             complement(2669..3346)
                     /locus_tag="AC158Q_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3340181.1"
                     /translation="MRILLVEDDMMLAEAVTRALVQAAHVVDCARTGGEADHALSVTD
                     YDLVLLDVGLPQIDGFEVLRRLRQRRSKVPVLMLTVKEALEDRIAGLDLGADDYLTKP
                     FHLSELEARVRALIRRAHAGAGSELVHGRLRLDMAGRRLYYDERPLDLSGRELAVIEM
                     MLLREGKVVTKQQIIDNLYGWEDSSSSNTVEVFVYRLRKKLESCGADININTVRGMGY
                     LIEKAHA"
     gene            complement(3689..4543)
                     /locus_tag="AC158Q_10180"
     CDS             complement(3689..4543)
                     /locus_tag="AC158Q_10180"
                     /inference="COORDINATES: protein motif:HMM:NF012976.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase family protein"
                     /protein_id="HIR3340182.1"
                     /translation="MRKVTKDTLTGAVIASFAGTKDERLKFVLARLVTHLHAFAKDAK
                     LTHAEWKAAIDFLYAAGKISSENRNEFMLASDVLGLSALVDLLNQPAGATEGSELGPF
                     YAEGSPLLPLGADLVKDNAGERVLVRGRVLDTGGRPIAGALLDFWQAADNGLYPEQDK
                     RQDPFNLRLRMKTDGEGRYAFTTIKPAPYTVPYDGPVGELLRAGGRDAWRPAHFHFIV
                     SAGGYLPVVTELFSEGDPCVERDAVFGVRESLVVRYVRHESAADAAPWKIAAPFFTVD
                     FDFRLARA"
     gene            complement(4619..>5466)
                     /locus_tag="AC158Q_10185"
     CDS             complement(4619..>5466)
                     /locus_tag="AC158Q_10185"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HIR3340183.1"
                     /translation="ASPSGGLDIMARTVGQPLSVMWGQPVVVDNRPGAGIMLGTEMAS
                     KAAPDGYTLIVLNANLAPNVVLHDKHAVVKGLTGVIKIADLPNAMSVPASLPVGSMKE
                     LIAMAKTARLTYGSAGHGTVGNILTEMLKLAARVDITHVPYKGGGPVMAAVTGGQVSL
                     GVVSLASTMAHMKAGRVKILGVSSSKRSRLAPDIPTIAETVPGVALESWVGMMAPAGT
                     PPAIIRSVNAAVLKAVEMPDVRKRLVSQGYDVQGTTPEVFNQLVQSDLKIYARVIREA
                     NIKIN"
BASE COUNT          915 a         1890 c         1765 g          896 t
ORIGIN      
        1 aggtccggcg gatccagtgg tatccgggcg gcgcgggctc ctccgcggcc atcggcgcgg
       61 gcgcggcttc aggcctgacc gtggcctcag ggatgagccc atcggcggac tcgcccgcgg
      121 acccggcggc cttccgcttc gccagcatcg tgcccaccat taccaccatg atcgcgccca
      181 gcagcggcgc cgcgctatgc agccattccg cgtcgccctg cacgaacggc ccgaccaccg
      241 ggtcggcgat cgccatctcg cccgccaccc agccgagcac gccgccgccc acggtgatga
      301 tcgccggcac gcggttcatc agcttcagga taaaggtgct gccgtagatg atgagcggga
      361 tgctcagcgc caggccgaga atcagaagca cgagattgcc cttggccgcg gccgcgacgc
      421 cgagcacgtt gtcgaggctc atgatgaagt ccgcgacgat gatggtcttg atcgccgacc
      481 acagctgcgt gtgagcgtcg atcttctcct cttcgccctc cggtatcagc atctgtatgc
      541 cgatccaggc aagcagcacc gcgccgatca gcttgagcca cggcagtccg agcagcatga
      601 cggcgaagaa cgtgaggatc acgcgcaaca ggatggcgcc gaagctgccg aaaaagatcg
      661 cctgcttctg ctgcttcggc ggcagcgagc ggctcgccat cgcgatgacc acggcgttgt
      721 cgccggagag cacgatgttg atcatcatga tctggcccag cgttacccag aaaaacgagc
      781 ttcccagcat ctccattttc gaaccctccg tggttccgcc gcggcaaagc gccgcgcgac
      841 gttgctggag agtcagaata cggatgcagg cttaccgcca gccgtcatcc ggctgaacgg
      901 caaatggctg tttcatgaac gaaatatgac gtgcgggaaa cgccgcctca ggcgggaggc
      961 ggcgcgggga atctgacgct gaaggtggtg ccaacgccgt cggtgtcgtc gctcaacgca
     1021 atgtcggcgc cgtgcagccg cgcgatttcc tgggcaatgg caagtccgag gccgctgccg
     1081 tcggccgagt tgccgagcag gcggtgaaaa cgctcgaaca cccgctcgcg ctcgtgcgcc
     1141 gggatgcccg gcccgtcatc gttgacggcg accgtgggca ccggcttcgc gctcacgcgc
     1201 acggtaacgc gtccccctgc ggggctgtag cggatggcgt tgtcgagcag gttgtcgagc
     1261 agctcgcgca accgcgcggc atcgccgcgc accatgaccg gttccgcgct gccctcgaac
     1321 cccaggtcga tgctgcgctg gcgggactcg ggcacccagc cggttgccgc ctccagcgac
     1381 agcgcgttga gatcgagttg cgccagcgtc accgcgcgca ccgcttccgg ctcgttgcgc
     1441 gcgagggaca gcaactggga aaccagccgc gacagccgct cgagcgccgg ggacagggcc
     1501 tgcatcgccg cgcgcatgcg ctgggggtcg gcctcccgct gcgccagctc gaactgggcg
     1561 ttcaacgcgg tgatcggcgt cttcagctgg tgcgcggcgt cggaaatgaa ccggctttgc
     1621 agcgtgagca cccggtccag gcgcgccagc agttcgttga tcgagtgcag cagcgggctc
     1681 acctcgcccg gcacattgtc gacgaccacg gggctgcagt cgcgatgcga gcgcgacgcc
     1741 actgcccgct gcaggcgctg cagcggcgac aggccgcgca ccacgccggc ccagacgacg
     1801 acgccggcga tcagaatcag caaaacctga ggaatgacga cggagagcag gatctcgcgc
     1861 gcgagcacgt tgcgcttgac catcgtctcg gccgcgcgca ccaccgcggc gggccgcccc
     1921 gtcgcgccgt cggcgtcgag cgccagctcg acgacccgga ccgcctcgcc ttcggcctcg
     1981 ccgtcgtaga gggccggccg gcggcgcgcg gcgtcgcgtc gagccggcgc gatcgggaaa
     2041 cccgccacga cgcgtccccc ggcatcttcg acctggaaat agacccggtc cacggggtcg
     2101 gtcagcagca gtcgccgcgc ctcggcgggc atctcccgcg cgagcgcgcc gccgtcggcc
     2161 cgcttgaggt aaagcgacac ctcgcgcgcg atctcgacca gggtccggtc gtaggcgcgc
     2221 tgcgagaaat tcagcgccac ccagtagctg atgaaagtgt cggcggtcag cagcaggaac
     2281 agcggggcca gcagccaggt cagcagctgg gtgcgcagca gcggctgctc aacagaacct
     2341 atttcatgta taggcacgtg tgcgacggag gcgatgtggg atgcaggttt aggaggattg
     2401 cccgctgcaa tatgtcgata ttgcaagggc aaccgacgac atgatgcgcc cctgagcggt
     2461 cgcttcgcga ccgcggggcg tacccctgcc gaaatcggcg cgcaacggca aagccgttgc
     2521 ttggcgcccc gtccctctgg gttgcggcca agacggtcgg ctgcatcgtt gccctccttg
     2581 accatatcga catatggcct tcgtcgggcg cctcgcatcc ggtccgtcct ggccacaacg
     2641 catcacgcgc ctatacatga aataggttct atgcatgcgc tttttcgatc aggtagccca
     2701 tgccccgcac ggtgttgatg ttgatgtccg cgccgcacga ttcgagcttc ttgcgcaggc
     2761 ggtagacgaa aacttccacc gtgttgctgg aagaactgtc ttcccagccg tagaggttgt
     2821 cgatgatctg ctgcttggtg acgaccttgc cctcgcgcag cagcatcatc tcgatcactg
     2881 ccagctcgcg acctgacagg tcgagcggcc gctcgtcgta atagagccgg cggcccgcca
     2941 tgtccaggcg cagccgcccg tggaccagct cggacccggc gcccgcatgc gcgcgccgga
     3001 tgagcgcccg cacacgcgcc tcgagctccg acaggtggaa cggtttcgtc aggtaatcgt
     3061 cggcgccgag gtcgagtccg gctatgcggt cctccagcgc ttccttgacc gtcagcatca
     3121 gcaccggcac cttgctgcgt cgctggcgca atcgccgcag cacctcgaac ccgtcgatct
     3181 gcggcaggcc gacgtcgagg agcaccagat cgtagtccgt aaccgacagg gcatgatccg
     3241 cctccccgcc ggtgcgcgcg cagtccacca cgtgcgcggc ctggaccagc gcgcgcgtca
     3301 ccgcttcggc cagcatcatg tcatcctcga ccagcaatat gcgcaaggca cctcccgttc
     3361 gattgcgcgg cggccccgga tggagcggcc ccgcccctat tattcggcac cggcagcggg
     3421 cgaggcaact ttttaacagt aaagagtcat ttctcagtca ggttcggttc atgtttatgt
     3481 cagcaaacgg taatgtttcg tgccgcccgc acccgccggt ctccccgtcg gaagccatcg
     3541 tgaaatcgcg tcaaaaagcc tcaccaccaa gcgtgtcctg agcctgccga agtggcctgt
     3601 cctgagtgag tcgaagaggc gccaggcgcg caaaaaaact cacgattggc gcggttcctc
     3661 cgcggccttg acggcctgca gtacggcttc aggcgcgagc gagacggaaa tcgaaatcca
     3721 ccgtgaagaa cggcgcggcg attttccagg gcgcggcgtc cgccgcagac tcgtgccgga
     3781 cgtagcgcac caccagggac tcgcgcacgc cgaacaccgc gtcgcgctcg acgcaggggt
     3841 cgccctcgga aaacagctcg gtgacgaccg gcaggtagcc gccggccgac acgatgaaat
     3901 ggaaatgcgc cgggcgccac gcatcgcggc cgccggcgcg caggagttcg cccaccggcc
     3961 cgtcatacgg caccgtgtag ggcgcgggct tgatggtggt gaaggcgtag cgcccctccc
     4021 cgtcggtctt catccgcaag cgcaggttga acgggtcctg gcgcttgtcc tgctcgggat
     4081 agaggccgtt gtccgcggcc tgccagaaat cgagcagcgc gccggcgatc gggcggccgc
     4141 ccgtgtcgag cacgcgcccg cgcaccagca cccgctcgcc cgcgttgtcc ttgacgaggt
     4201 ccgcccccag cggcagcagc ggcgagcctt ccgcgtagaa cggccccaac tcgctgccct
     4261 cggtcgcgcc cgccggctgg ttcagcaggt ccaccagcgc ggagagcccc agcacgtccg
     4321 aagccagcat gaactcgttg cggttttcgc tcgagatttt ccccgcggca tagagaaagt
     4381 cgatcgccgc cttccactcg gcgtgcgtca gcttcgcgtc cttcgcgaag gcgtgaagat
     4441 gcgtcaccag ccgcgcgagc acgaacttga gccgctcgtc cttcgtgccg gcgaacgagg
     4501 cgatgacggc gccagtgagg gtgtcctttg ttactttacg catgcgctca tggagtgtcg
     4561 ggtgacgagt gacgagtgac gaattagacc cttccgcctc ccgccttccg acattcgcct
     4621 agttgatctt gatgttggcc tcgcgaatga cgcgcgcata gatcttgaga tcggactgga
     4681 cgagctggtt gaacacttcg ggcgtggtgc cctgcacatc gtagccctgg ctgacaaggc
     4741 gcttgcgcac atccggcatt tccacggcct tcaacaccgc ggcgttgacc gacctgatga
     4801 tcgccggcgg tgtccccgcg ggggccatca tgccgaccca gctctcgagc gcaacgcccg
     4861 gcaccgtctc ggcgatggtc gggatgtccg gtgcgaggcg cgagcgcttg ctgctgctca
     4921 cgccgaggat ctttacccgt cccgccttca tgtgcgccat ggtggaagcg aggctcacga
     4981 cgcccaggga cacctggccg ccggtcacgg cggccattac tggcccgccg cccttgtaag
     5041 gcacgtgcgt gatgtccacg cgcgccgcga gcttgagcat ttccgtcagg atgttgccga
     5101 cggtgccgtg cccggccgag ccgtaggtca gccgcgccgt cttcgccatc gcgatcagct
     5161 ccttcatcga cccgaccggc agcgaagccg gcaccgacat cgcgttgggc aggtcggcga
     5221 tcttgatgac gccggtcaaa cccttcacca ccgcgtgctt atcgtgcagc actacgttcg
     5281 gcgcgaggtt ggcgttcagc acgatgagcg tgtagccgtc gggcgcggct ttcgacgcca
     5341 tctccgttcc cagcatgatc cccgcgcccg ggcggttgtc cacgaccact ggttgccccc
     5401 acatcaccga gagcggttgc cccaccgtgc gcgccatgat gtccagaccg ccgctgggcg
     5461 acgccg
//
LOCUS       DBNGSG010000393         5674 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3352630, whole genome shotgun sequence.
ACCESSION   DBNGSG010000393 DBNGSG010000000
VERSION     DBNGSG010000393.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5674)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5674)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5674
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3352630"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="AC158Q_10190"
     CDS             <1..521
                     /locus_tag="AC158Q_10190"
                     /inference="COORDINATES: protein motif:HMM:NF027681.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3340184.1"
                     /translation="QPICSYGIHKLAIEKHLHLNWLLHGLDYCILRISNAYGERQRTD
                     TAQGTVPVFLDRALRGKPIQIWGDGSVVRDYIYVGDIVEALLLAPAYEGAQKIFNIGS
                     GTGVSLNQLIKEIGWVTGRGLSVEYTPARSFDVPANVLDTSLAQRFLGWKARTPLRDG
                     LRRTYDWMQRSR"
     gene            518..1696
                     /locus_tag="AC158Q_10195"
     CDS             518..1696
                     /locus_tag="AC158Q_10195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3340185.1"
                     /translation="MSVGAAQGASRIRVVLVTHYFPAHSGGVERVAGELASRLVRNGT
                     MQIEWHASECDLAPPDTPGVKCLPARSCNAIERFLGFPYPLWSPGALWRVARAARRAD
                     VVHLHDCLYLPNLVAYAAARLARRPVLVTQHVGFVPYRSSVLRAMLAAANRIFGSVVL
                     GGATQVVFESDTVRRYFLRFVRFRAEPLLVPNGVDTGEFTPAASPRRQSLRAELGARD
                     GRPLLLFLGRFVEKKGLAVLRELTERIPHAQWVIAGWGPLDPAAWGRQNVTVVHRPGS
                     DQLAPLYQAADLLVLPSVGEGFPLSVQEAMACGTPALVGDETAAGCPESQDVLLREAV
                     GAQDTAARWAGRIEGLLASPATLETLRPRVAEFARARWSWEKCAGRYSGILHGIRNGQ
                     "
     gene            complement(1733..2551)
                     /locus_tag="AC158Q_10200"
     CDS             complement(1733..2551)
                     /locus_tag="AC158Q_10200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3340186.1"
                     /translation="MNGTSPLVLVLIPVYGGGEALRASLASLSLARLPERLCTLVVDD
                     GSDPPIAEAPAHHPRLGLHVERLARHAGIEAALNRGLEIGRELGADYIARLDAGDTIH
                     PDRLVKQLEILQRDGDVGIVGTDAWFVDEQSRPIFRFETPRTDGQVRRRMHLGCCLLH
                     PTVMIRRSAIEQAGTYSPEYPAAEDYDLFFRLIGRTRAATVPEPLTTKVLARDSVSLT
                     KRRTQLRSRLRVQRRHFDPGRWESYAGVALTLSLFLIPNRLVIAIKRLAGISRI"
     gene            complement(2548..3375)
                     /locus_tag="AC158Q_10205"
     CDS             complement(2548..3375)
                     /locus_tag="AC158Q_10205"
                     /inference="COORDINATES: protein motif:HMM:NF016908.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HIR3340187.1"
                     /translation="MSPEPDLVFDVGMHNGDDTAYYLHKGYRVVAVEANIGLVERARR
                     RFAPALEHGTLRILNVGITAEAGEAEFFISPAHDFWSSFDRQLAARAGAYRTVRVACT
                     PFAPLLEAHGVPYYLKIDIEGNDHLCLGALERVSLPAYVSIEMSHENGGRDIERLAAL
                     GYRRFQCIRQTDYAPMTPESAERSATLRRAYSRWKPAHFAVLAGRRIQRTLRPPSDGA
                     WNFPKGSSGPFGAGLAGPWLPVANVLEVWRILRDVDLELGNHGLGEWFDIHAAAAPA"
     gene            complement(3494..4762)
                     /locus_tag="AC158Q_10210"
     CDS             complement(3494..4762)
                     /locus_tag="AC158Q_10210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3340188.1"
                     /translation="MKILIVSRSDARGGAARAGYQLHRALNANGQTSRMWVGVKDSTD
                     PTVTGPLGALATAWSQIRPGLAITLMRRLQRAPDRAWRSLALLPSGLGRAVNRSDADI
                     VNLQWVGDETLSVTEIGCIAKPVVWTMHDMWPFCGAEHYAPDDASARWRGGYTPGTRA
                     AGAGGFDIDAWVWRRKRDAWRPCRLVVASRWLADCASTSALMRGWPVSVIPHVVDTNV
                     FRPHDDASARAEWDLPPDVPIVLFAAAGGSRDPRKGWDLLVSALDVVAATRRDMVCAV
                     LGENESSAPRWTLPVPVRWLGSVSDDAALARLYAIADVTVVPSRQDNLPLVAIEAQAC
                     GCPVVAFAATGLPDAIEHRLTGYLAEPFASADLARGILWVLADKQRHQQLRAQARERA
                     RRLWSPGVVTRQYCEVYAAAIEAYRAKAAR"
     gene            complement(4782..>5674)
                     /locus_tag="AC158Q_10215"
     CDS             complement(4782..>5674)
                     /locus_tag="AC158Q_10215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3340189.1"
                     /translation="PAATPRPAIPPVAPAARPVIDASLVVTVLNEAGSIEELLTTLAA
                     QTVWPREIVVVDGGSTDGTLPLLRRHEQLMARWLSEPDGGIYDAWNKACGMARGEWLL
                     FLGAGDEFASPGTLADLSVHLSAAHPAHDLVYGKLIYISQAGRRDLEEVGKPWAELAG
                     RWELGRPALPPHPATFHHRSLFAGGRGFDASYRLAGDAHFLLRYALRKPPLHVPLVVC
                     RTPVGGVTMNLRNVLALAREIRAINRELALVPPLRHRLIEALLLGAKVVVGLMPAPMG
                     HRIADWYRRILGQPQRWSVR"
BASE COUNT          972 a         1944 c         1844 g          914 t
ORIGIN      
        1 cccagcccat ctgctcctac ggaattcaca aacttgccat cgagaaacat ctacacttga
       61 attggctgct tcacgggctt gattactgca tcctccgcat ctcgaacgca tatggcgagc
      121 ggcagaggac cgacacggct cagggaaccg ttccggtctt tctcgaccgg gccttgcgcg
      181 gaaagccgat tcagatctgg ggggacggct cagtggttcg ggattacata tatgtcggcg
      241 atattgttga agcgcttctt ttggctcctg cgtacgaagg agcgcagaag atattcaaca
      301 tcgggtccgg aaccggcgtc agcctgaatc aactcattaa ggaaatcggg tgggtaaccg
      361 gtcgcggcct ctctgtcgag tacacgcctg cgcgtagctt cgacgtgccg gcaaacgttc
      421 tggacacttc tctagcccaa cggttcctcg gctggaaggc cagaacacct cttcgcgacg
      481 gtttgcgtcg cacctacgac tggatgcagc gctcgcgatg agcgtgggcg ccgcgcaggg
      541 cgcgtcgcgg atccgcgtcg tgcttgtaac ccactacttc ccggcacaca gtggcggcgt
      601 ggagcgcgtt gccggcgagc tggcttcccg gctcgtgcgc aacggtacca tgcaaatcga
      661 atggcatgcg agcgaatgcg acctggcgcc gcccgacacg ccgggggtga aatgcttgcc
      721 ggcgcgaagc tgcaacgcga tcgagcgctt tctgggcttc ccctacccgc tctggtcccc
      781 cggcgcgctg tggcgcgtgg cgcgggcggc gcgccgtgcc gatgtcgtcc acctccacga
      841 ttgcctgtac ctgcccaacc tggtggctta cgccgccgcc cgccttgcgc gccgcccggt
      901 gctcgtcacg cagcacgtgg gcttcgtgcc gtaccgaagt tccgtcctgc gggcaatgct
      961 tgccgcagcg aaccggattt tcggctccgt cgtgctggga ggcgcgacgc aagttgtctt
     1021 cgagagtgac acggtccgcc gttacttctt gcgcttcgtg cgcttccggg ccgagccgct
     1081 gctcgtgccg aacggcgtgg acaccgggga attcaccccc gccgcctcgc cgcgcaggca
     1141 gtcgctgcgc gccgaacttg gcgcgcggga cggcaggccg ctgctcctgt tcctgggacg
     1201 attcgtcgaa aagaaaggcc tggcagtgct cagggaactg acggaacgaa tcccgcatgc
     1261 gcagtgggtc atcgccggtt ggggcccgct cgaccccgcg gcgtggggca ggcagaacgt
     1321 tacggtcgtg catcgacccg ggagcgatca actcgcgccg ctttaccagg cggccgatct
     1381 gctcgtcctg ccgagcgtcg gagaggggtt cccgctctcc gtgcaggaag ccatggcttg
     1441 cggcacgccc gccctcgtcg gggacgagac cgccgcaggc tgtcctgagt cccaagacgt
     1501 gcttcttcgg gaagcggtgg gtgcgcagga cacggcggcc cgttgggcgg ggcgtatcga
     1561 agggctcctg gcttctccgg cgacgctcga aaccctgaga ccaagggttg ccgagttcgc
     1621 gcgcgcgcgc tggtcgtggg agaaatgcgc tggccggtac tccgggatcc ttcatggcat
     1681 tcgtaacggg caatagaact cgcaaaggag tcacgaccgt ctcctcgccc cctcaaattc
     1741 tggaaatgcc cgccagccgc ttaatcgcaa tcaccaggcg attcggaatc aggaagagcg
     1801 acagcgtcaa ggccactccg gcgtagcttt cccagcgccc cgggtcgaaa tgccggcgtt
     1861 gcacgcgcaa gcgcgaccgc agctgggtgc gccgcttcgt gagggaaacg ctgtcgcggg
     1921 ccaggacctt ggtggtcagc ggctccggaa ccgtcgcggc acgcgtgcgg ccgatgagac
     1981 ggaagaataa atcgtagtcc tccgccgccg ggtactcggg cgagtacgtt cctgcctgct
     2041 cgattgcgct gcgcctgatc atgaccgtgg gatgcagaag gcagcagccc aggtgcattc
     2101 gccgccgcac ctgtccatct gtgcgcggcg tctcgaagcg gaatatcggg cggctctgct
     2161 cgtccacgaa ccaggcgtcg gtgccgacaa tgccgacatc gccgtcccgc tgcaggattt
     2221 ccagctgctt caccagcctg tcaggatgga tcgtgtcgcc cgcgtcgaga cgcgcgatat
     2281 agtcggcgcc cagctcgcgg cctatctcga gaccgcggtt caaggcggct tcgataccgg
     2341 catgccgtgc caggcgttcc acgtgcaggc ccaaccgcgg gtgatgcgcg ggtgcctcgg
     2401 caatgggcgg atcgctgccg tcgtccacca ccagcgtgca caaccgctcg ggcaggcggg
     2461 cgagagaaag cgaggccagg ctcgcgcgca acgcctcgcc gccgccataa accggaatga
     2521 gcaccagcac cagaggagaa gtcccgttca tgccggcgct gctgcggcgt gaatgtcgaa
     2581 ccattcgccg aggccgtgat tgccgagttc cagatcgacg tcgcgcagga tgcgccagac
     2641 ctcaagcacg ttcgcgaccg gcagccacgg cccggcaagg ccggcgccga atggaccgct
     2701 cgagcccttc gggaaattcc aggcgccatc ggacggcggc cgcaaggtcc tctggatgcg
     2761 gcgcccggcc aggaccgcga agtgcgccgg cttccagcgg gaatacgcgc ggcgcagcgt
     2821 ggcggagcgc tccgcgctct ccggcgtcat cggtgcgtaa tccgtctggc ggatgcactg
     2881 gaaacggcgg tagccgagtg ccgcaagcct ctcgatgtcg cgaccgccgt tctcgtggga
     2941 catctcgatc gaaacgtagg cgggaagcga aacccgttcc agcgctccca ggcacaggtg
     3001 gtcgttgccc tcgatgtcga tcttgagata gtacggcacg ccgtgcgctt cgaggagcgg
     3061 cgcgaacggc gtgcaggcca cgcgaaccgt ccggtatgcg ccggcgcgcg cggcgagctg
     3121 tcggtcgaaa gagctccaga aatcatgcgc cggcgaaatg aaaaactcgg cctcgcccgc
     3181 ttcggcggtg atgccgacgt tcaggatccg cagcgttccg tgctcgagcg cgggagcgaa
     3241 ccgcctgcgc gcgcgctcca ccagtccaat gttcgcttcg accgcaacca cccggtaacc
     3301 cttgtgcagg tagtaggcgg tgtcgtcgcc gttgtgcatg ccgacatcga ataccagatc
     3361 gggctcggga ctcattttgc cggccctctc ctccgcagcg gcgaaccgtc ccgcgagccg
     3421 cgcatgccaa tcgaccgcgg atttccgcgg tacccgagga accgcaggaa gatactgcac
     3481 ccgcagaacg cgctcaccgg gccgccttcg cgcgatatgc ttctatggcg gcggcataga
     3541 cctcgcagta ctgccgcgtg acaacgccgg gagaccatag gcgccgggcc cgctcccgcg
     3601 cctgggcgcg cagttgttgg tggcgctgct tgtcggccag cacccagaga atgccgcgcg
     3661 cgaggtccgc ggacgcgaac ggctccgcca ggtagccggt caacctgtgc tcgatcgcat
     3721 cgggaaggcc ggtcgcggca aaagcgacca ccgggcatcc gcaggcctgc gcctcgatgg
     3781 caactagcgg aaggttatcc tggcgcgagg gcacgacggt gacatcggcg attgcgtaca
     3841 gacgcgcgag cgctgcgtcg tccgacacgc tccccagcca acgcaccggc accggcagcg
     3901 tccaccgcgg cgcgctcgac tcattttcgc cgagcactgc gcagaccata tcgcgccgcg
     3961 tcgccgccac gacatccagc gccgacacca gcagatccca acctttacga ggatcccgcg
     4021 atccaccagc cgcggcgaag agcacgatcg gcacgtccgg cggcagatcc cactcggcgc
     4081 gcgccgatgc gtcatcgtgc gggcggaata cattagtgtc gacgacgtgg ggaatcaccg
     4141 agacgggcca gccccgcatg agcgcgctgg tgctcgcgca gtcggcaagc catcggctcg
     4201 caacgacgag ccggcatggc cgccaggcgt ctcgcttgcg tcgccacacc caggcatcga
     4261 tatcgaaacc gcctgccccg gccgcccgcg tgccgggcgt gtagccgccc cgccaacggg
     4321 cactggcatc gtcgggtgca taatgctccg cgccgcagaa cggccacatg tcatgcatcg
     4381 tccacacgac cggcttcgcg atgcagccga tttccgtcac cgacaacgtc tcatcgccga
     4441 cccactgcag gttgacgata tcggcatcgc tgcgattgac cgcccggccc agtcctgatg
     4501 gaagcaacgc cagcgaccgc catgccctgt ccggcgccct ctgcaggcgg cgcataaggg
     4561 ttatcgcgag cccggggcgt atctggctcc atgcggttgc gagcgccccg aggggtccgg
     4621 tcaccgtggg atccgtggaa tccttgactc cgacccacat gcggctcgtc tgcccgttcg
     4681 cgttgagcgc gcggtgaagc tggtagccgg cgcgtgccgc gcccccgcga gcatccgaac
     4741 gactgactat cagaattttc accgcctggt cgcccaaatt attaacgtac gctccatctc
     4801 tgcggctgcc cgaggatgcg cctgtaccaa tcggcgatcc tgtgtcccat cggtgcgggc
     4861 atgagcccaa ccaccacctt ggcaccgagc agcagcgcct cgatcaaccg atggcgcaga
     4921 ggcggaacca gcgccagttc ccggttgatg gcgcggatct cgcgcgcgag cgcgagcacg
     4981 tttcgcaggt tcatggtcac gcccccgacg ggcgtgcggc agacaaccag gggaacgtgc
     5041 agcggcggct tgcgcaacgc gtagcgcaga aggaaatgag cgtccccggc caggcggtaa
     5101 ctcgcgtcga atccccgtcc gccggcaaac agcgagcgat ggtgaaacgt tgccgggtgc
     5161 ggcggcaagg ccggacggcc gagttcccag cgacccgcca gctccgccca cggctttccg
     5221 acctcctcga gatcccgcct gccggcctga ctgatataga tcagcttgcc atacacgaga
     5281 tcgtgggcgg gatgcgcggc ggaaagatga accgagaggt ccgccagcgt gcccggtgag
     5341 gcgaattcgt caccggcgcc aaggaacaac agccactctc cgcgcgccat cccgcacgcc
     5401 ttgttccagg cgtcgtaaat cccgccatcg ggctcgctca accagcgggc catcaactgc
     5461 tcgtgccgcc gcaacagcgg tagcgtccca tcggtcgagc caccatcgac gacgacgatc
     5521 tcgcgcggcc agaccgtctg cgccgccagc gtggtcagca gttcctcgat actgccggcc
     5581 tcgttgagaa cggtcaccac caggctggcg tcgattaccg gccgcgcggc gggcgcaacc
     5641 ggcgggatcg ccggccgcgg cgtcgcggcc ggtc
//
LOCUS       DBNGSG010000394         3320 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2673305, whole genome shotgun sequence.
ACCESSION   DBNGSG010000394 DBNGSG010000000
VERSION     DBNGSG010000394.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2673305"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..1487)
                     /gene="phoR"
                     /locus_tag="AC158Q_10220"
     CDS             complement(171..1487)
                     /gene="phoR"
                     /locus_tag="AC158Q_10220"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF008235.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HIR3340190.1"
                     /translation="MHLLARTLAVIAIFAIPALAVGAAFGAAWGWALLSLLLAGLVIN
                     HARHLRLLEKWAAGSLSETVPEGSGTWRAAFTLIYQRQRAEIRRRRQLARALARSRQA
                     GRALPYGVAILDADYRILWCNDSCEAHFDIDGKSDARQPITNLARQPEFVSYAAARDF
                     SAPLELRTGRDDGLVLSVQFVPYEESQWLLLSRDVTQASRLEAVRRDFVANVSHELRT
                     PLTVLVGFLETLRERDVEPKQSRRFFDLMDEQGRRMQRILDDLLVLSTLESSPEPPRE
                     ERIAVAGLLGRIRGETEALSAGRHRIQLDETPGYDLLGADAELSSAFGNLASNAIRYT
                     PAGGEVRLVWRAGPDGAVFSVEDTGIGIEREHIPRLTERFYRVDRGRSRETGGTGLGL
                     AIVKHTLARHQATLEIESEPGKGSRFTARFPARRVVPALPPAAGAK"
     gene            complement(1497..2192)
                     /gene="phoB"
                     /locus_tag="AC158Q_10225"
     CDS             complement(1497..2192)
                     /gene="phoB"
                     /locus_tag="AC158Q_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HIR3340191.1"
                     /translation="MAAASILGVEDEFAIQELIAVNLEHAGHTVLRAASAAQALELIR
                     ESLPDLVLLDWMLPDRPGVEFARQLRADRRTRDIPIIMLTARAQEQDKVSGLESGADD
                     YVTKPFSSRELLARIQAVLRRRAPELAGEILEINGLALDPVSHRVTARGASVDLTPTE
                     FQLLRFFMAHPERVYTRQQLLDQIWVDHVFVDERTVDVHIRRLRKALEPTALADLVET
                     VHGTGYRFRNDQP"
     gene            complement(2246..2962)
                     /gene="phoU"
                     /locus_tag="AC158Q_10230"
     CDS             complement(2246..2962)
                     /gene="phoU"
                     /locus_tag="AC158Q_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HIR3340192.1"
                     /translation="MASEHFSKQYDAELEALRSRVLQMGGLVESQIRKAIDAFATGSQ
                     DLIDEVVAAELRVNEAELNIDDDLSHIVVKRQPAANDLRLIMAISKTVTDLERIGDEA
                     EKIARMSRGIHDGAVRLQIHSYANIRHAADIALRMLRQALDAFARQDATQAAGVIRED
                     VAIDNEFRSILRQLITFMMEDPRTISMALEIVWIAKAIERIGDHAKNVAEDVIYIVKG
                     TDVRHIKLEEVERQALGGPS"
     gene            complement(2980..>3320)
                     /locus_tag="AC158Q_10235"
     CDS             complement(2980..>3320)
                     /locus_tag="AC158Q_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HIR3340193.1"
                     /translation="RLHQQAFNLSGGQQQRLCIARALATSPEILLFDEPTSALDPIAT
                     ASIEELIHNLKKEVTILIVTHNMQQAARVSDFTAYMYLGEMMEFGVTDEIFMKPARKE
                     TEDYITGRFG"
BASE COUNT          534 a         1115 c         1091 g          580 t
ORIGIN      
        1 cgcgcgccgg ccgtgcagga acgtttttcc tcccaggggc tggaactcca cgtttcaacg
       61 ccggaagact tccgcaatca tctccggcac gaaatatcca aatgggacaa ggtggtccgg
      121 tccgccggcg tcaaggcgga ataactgcgg attcgatgcc tgaaccagcc ctatttcgct
      181 ccggccgccg gcggcagcgc gggcacgacg cggcgcgccg gaaagcgcgc ggtgaagcgg
      241 ctgcccttcc ccggctcgct ctcgatctcc agggttgcct ggtgccgtgc aagcgtgtgc
      301 ttgacgatcg ccagacccag cccggtgccg ccggtttcgc gcgagcgccc gcgatccacg
      361 cggtagaacc gctcggtgag cctcgggatg tgttcccgct cgatgccgat tccggtgtct
      421 tccaccgaga aaaccgcgcc gtccgggccg gcgcgccata ccaggcgcac ttcgccgcct
      481 gcgggcgtat agcggatggc gttgctcgcc aggttgccga aagcgctcga cagctcggcg
      541 tcagcgccca gcaggtcgta tcccggcgtc tcgtccagct ggatgcgatg gcggccggcg
      601 gacagcgcct cggtttcgcc gcgaatgcgg cccagcagcc cggccaccgc gatccgctcc
      661 tcgcgcggag gctccggcga ggactccagc gtggacagca cgagcaggtc atccagtatc
      721 cgctgcatgc gcctgccctg ctcgtccatc aagtcaaaaa accgccgtga ctgcttcggc
      781 tcgacatcgc gctcgcgcag cgtctccaga aagccgacga gcaccgtgag cggcgtgcgc
      841 agctcgtgcg agacgttcgc cacgaaatcg cgccggaccg cttccagccg cgacgcctgc
      901 gtcacgtcgc gggacagcag cagccactgc gattcctcgt agggcacgaa ttgcacggag
      961 agtaccagtc cgtcgtccct gcccgtcctc agctccagcg gcgctgaaaa atcgcgcgcg
     1021 gcggcgtagc tgacgaactc cggctgacgc gccaggttgg tgatcggctg gcgcgcgtcc
     1081 gatttgccgt cgatatcgaa gtgcgcttca caggaatcat tgcaccacag gatgcggtaa
     1141 tcggcatcca ggatggccac gccgtaggga agcgcgcggc cggcttgccg ggagcgggcg
     1201 agcgcgcgcg cgagctggcg gcggcgccgg atttccgcgc gctggcgctg gtaaatcagc
     1261 gtgaaggcgg cccgccatgt cccgctgcct tccggaaccg tctcggacag cgatcccgct
     1321 gcccacttct ccagcagccg caggtggcgc gcatggttga tgaccaggcc ggcgagcaac
     1381 agggacagca gcgcccagcc ccaggcggcg ccgaacgccg cccccaccgc cagcgcggga
     1441 atggcgaata tcgcaatgac tgcgagagtg cgcgccagca gatgaatggg aacgcgctaa
     1501 ggttgatcgt tgcgaaaacg ataaccggtg ccgtgcacgg tttcaaccag gtcggccagc
     1561 gccgtgggct ccagggcctt gcgcagccgg cggatgtgga cgtccacggt ccgctcgtcg
     1621 acgaatacgt gatcgaccca gatctggtcg agcagctgtt gccgcgtata aacacgctcc
     1681 gggtgggcca tgaagaatct caggagctgg aattcggtcg gggtgagatc caccgaagcg
     1741 ccgcgggcgg tcacgcggtg cgacaccggg tccagggcga gtccgttgat ttcgaggatt
     1801 tctccggcga gctcgggcgc ccgccggcgc aggacggcct ggatgcgcgc gagcaattca
     1861 cgcgacgaga acggcttggt gacatagtcg tccgcgccgc tctcgagtcc ggacaccttg
     1921 tcctgttcct gcgcccgggc ggtgagcatg atgatcggga tatcgcgggt ccgccggtcg
     1981 gcgcgcagtt gccgcgcgaa ttcgacgccc ggccggtcgg gcagcatcca gtccagcagc
     2041 accaggtccg gcagcgattc gcggatgagt tccagcgcct gcgcggcgct ggcggcgcgc
     2101 aaaaccgtgt gccccgcatg ctccaggttg accgcgatca gctcctggat ggcgaattcg
     2161 tcctccaccc cgagtatcga tgcggcagcc atcgtttccc gcctctcttt ccgggcccgc
     2221 accggaggcg tccccgcggc cgccttcacg acggcccgcc gagcgcctgg cgctccactt
     2281 cttccagctt gatatgccgc acgtcggtgc ccttgacgat atagatgacg tcctcggcca
     2341 cgttcttggc gtgatcaccg atgcgctcga tcgccttggc gatccacacg atctcgagcg
     2401 ccatggagat ggtgcgggga tcctccatca tgaaggtaat gagctggcgc aggatggagc
     2461 ggaactcgtt gtcgatggcg acatcctcgc ggatcacgcc ggcggcctgc gtggcatcct
     2521 gccgcgcgaa cgcatcgaga gcctgcctca gcatccgcag cgcgatgtcc gcggcatgcc
     2581 ggatattggc gtagctgtga atctgcaggc gcacggcgcc gtcgtggatc cccctggaca
     2641 tccgggcgat tttctccgcc tcgtccccga tgcgctcgag gtcggtcacc gtcttgctga
     2701 tcgccatgat caggcgcagg tcgttggcgg cgggctgccg cttgacgacg atgtggctca
     2761 agtcgtcgtc gatattgagt tcggcctcgt tcacgcgaag ctccgccgcc accacctcgt
     2821 cgatcaggtc ctggcttccg gtcgcaaaag catcgatcgc cttgcggatc tgcgactcga
     2881 cgaggccgcc catctgcagg acgcgggagc gcagggcttc cagctcggcg tcgtactgct
     2941 tggaaaaatg ctcgctggcc atgatgtctc ccgtccgggc taaccgaacc gcccggtgat
     3001 gtagtcctcg gtttccttcc ttgccggttt catgaagatt tcgtcggtca cgccgaactc
     3061 catcatttcg ccgaggtaca tgtaggcggt gaaatcggac acccgcgccg cctgctgcat
     3121 gttgtgcgtg acgatgagta tggtcacttc cttcttgaga ttgtggatca gctcctcgat
     3181 gctcgccgtc gcgatcgggt cgagcgccga ggtcggctcg tcgaacagca ggatctccgg
     3241 gctggtcgcg agcgcccgcg cgatgcacag gcgctgctgc tgcccgcccg acaggttgaa
     3301 ggcctgctgg tgaaggcggc
//
LOCUS       DBNGSG010000395         6423 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4203193, whole genome shotgun sequence.
ACCESSION   DBNGSG010000395 DBNGSG010000000
VERSION     DBNGSG010000395.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6423)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6423)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6423
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4203193"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..2272
                     /locus_tag="AC158Q_10240"
     CDS             143..2272
                     /locus_tag="AC158Q_10240"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024938.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043758 - acetate-CoA ligase (ADP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HIR3340194.1"
                     /translation="MTSLERFFSPRSVALVGATDDTTRFGGRLLRQMLKFGFGGRILP
                     VNPRRDRLGDLPCFPTVADLPEAPDHAGIVVPADKVLPVLRECHARGIAYATVFTAGF
                     SETGTPEGRAMQDAITGFARESGLRVMGPNCYGVINYPDHFALTASSVLEPDARRDGT
                     IGVVSQSGGLGGINVMWRAMEAGLRINYSTSSGNEADLDAIDFAGYMIEAPSTEVVLM
                     ALEAVRDGAKFARMAERAAELEKPIVVLKLGRTGHGSRAAASHTGAMTGADDVFEAAA
                     RQFGVKRVNDSRELYETAIMLRGRRWPRGRRAASLSLSGGNVVQVADVGAQLGLEWPD
                     YTATTQAALRGLLPGYGSVGNPTDTTSLASGQPELFRRTLELIARDGNVDVMVPVFTV
                     PMRAELELGVQIAKESAKPVAVLLTGKCLDDPSFTVERIVADGVPAYRDVVTCLAAVR
                     AAVDYREFLDRFRRQDNYSRPAGTDSKGARAQLAGSMNAGAGQAPLTERESRKVLQCY
                     GLPVTADRLARNAGEAIAHARDIGAPVALKIESPQIAHKTEAGAVRLNLSNPMEIRTA
                     YDAVVAAARRYNAGATINGVLVQPMAPRGIEMMLGVATDPVFGPVIAVALGGIYAELM
                     RDIAYRVAPVDAAQALAMLRELRAYRLLEGVRGQPRSDIETLTDCIVRLSWLAHDLRD
                     AVTDVDVNPLLVFERGALAIDALVVRR"
     gene            complement(2375..3169)
                     /locus_tag="AC158Q_10245"
     CDS             complement(2375..3169)
                     /locus_tag="AC158Q_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR3340195.1"
                     /translation="MSDLEYTVKDGVATARLNRPAKKNALSDDMVDGLAHALREAQDD
                     PAVRVFILTGAGDAFCSGGDLGRRSKEGENATPTPLQRKTRLQQVTHQAALAAEAFDK
                     PLLAAVNGPAAGAGMDLALMCDLRFAARSARFSEAYIRVGLLPGNGGAYYLPRIVGTA
                     AALELLWTGEFIKANEALRIGLVSRVYEDDRLMPETQAFAARLAAGPPIQQRLIKKLL
                     YESLRTDLRTSLDLVSSHMAVVQSTDDYREAIAAYKEKRAPKFKGR"
     gene            complement(3195..3737)
                     /locus_tag="AC158Q_10250"
     CDS             complement(3195..3737)
                     /locus_tag="AC158Q_10250"
                     /inference="COORDINATES: protein motif:HMM:NF013970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HIR3340196.1"
                     /translation="MNARVFVDTNVFVYARDPRDAAKQARAEDWVANLWRDRAGRTSM
                     QVLSEYYYNVTRKLLPRVAPESAWDDTRNLLDWNPHLVDATLLARAREIEQRWRLSWW
                     DSMIVAAAQLQGCALLLTEDLQDGAAYGGVTVRSPFTLDVREPVPAYAVAPAATPRHR
                     GRGRPRKPEGERRKAKVENP"
     gene            complement(3743..3997)
                     /locus_tag="AC158Q_10255"
     CDS             complement(3743..3997)
                     /locus_tag="AC158Q_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HIR3340197.1"
                     /translation="MKNITITLDKETAHLARVRAAERDMSVSRYIGELLRKDIRESDR
                     YQEAMRSYLSRGPFKELTGPPQRYATREELHDRDALRPKK"
     gene            complement(4116..5075)
                     /locus_tag="AC158Q_10260"
     CDS             complement(4116..5075)
                     /locus_tag="AC158Q_10260"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3340198.1"
                     /translation="MKMKLAMTMVSVLAFITCAQAAQDYPVRPIRVIVPQAPGGSNDI
                     MARYIGGALSERVGRQVVVDNRPGADGTIGTEIVARANPDGYTLLMASTAFVMNPAVI
                     KKLPYDPIRNFDWVATFGKAPVVITVGPAAKVNSLQELLALAKSKPNYITMASAGGFM
                     HFVSAMFRSHAGFKGEIALYKGGAPALLDVMSGQAHMAVATIPTSNAHIRSGRIKALA
                     VGATKRASSLPDVPTSVEAGLPSYQAEIWWAWATTAGTPQAVINKLNKEIAAILAMPE
                     TAKRFAVDSAEVDIRTPAQIRKMIPAELAKWAKVAQDAGMPKQ"
     gene            complement(5155..6183)
                     /locus_tag="AC158Q_10265"
     CDS             complement(5155..6183)
                     /locus_tag="AC158Q_10265"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3340199.1"
                     /translation="MIDMHAHWRPAELADALRARTRNPRIERNPDGVEVLKSSRYGEE
                     PLSRAFDDVAFHLGRMDRQGVSTSVLSLLGSFCWIEAQPLEVSVPLCRLVNDTLSGIC
                     QAHKGRFLAHAALPLTDIAVAAAELDRALGLPGMVGAQIPGNGFLTRKDAEKMRPLLE
                     VANRHHALLFIHHGPLPGDPFPRHVGDTDNMRRRNGTLDMQASLSSAMMTLCLTDILA
                     PYPDAAIYLHNLGGNIAFEVERMDHRSLLDTPDEELPSARFRRARVYVDCNSFGPRAI
                     EAGVRLYGAERVVCGTDGTEFGCDWTRKALDEANIGEEERHRILEGNAAAMLSRRAAV
                     APRERAAA"
BASE COUNT          978 a         2140 c         2232 g         1073 t
ORIGIN      
        1 gcgagatcga gcgcttcgcg aagctcgcca ggagcgcggg cctgaagaag gaatgagaag
       61 gtcaagtcga aggtcgaaag tcgaaaggcg aaagtaggtt cgttccattc ctcatgatcc
      121 tgtcaaccct gacccttcgg ttttgacttc gctcgagcgc tttttctcgc cgcggtcggt
      181 cgcgctggtc ggcgccacgg acgacaccac gcgcttcggc gggcgcctgc tgcggcagat
      241 gctgaagttc ggcttcggcg gccgcatact ccccgtcaat ccccggcgcg accggctcgg
      301 cgacctgccg tgctttccca cggtcgccga cctccccgag gcgcccgatc acgccggcat
      361 cgtggtgccg gcggacaagg tgctgccggt gctgcgggaa tgccacgcgc gcggcatcgc
      421 ctacgccacc gtcttcacgg caggcttcag cgagaccgga acgcccgaag gccgcgcgat
      481 gcaggacgcg atcaccgggt ttgcccggga gagcggcctg cgggtcatgg gcccgaactg
      541 ctacggcgtc atcaattatc cggaccactt cgcgctcacc gcgagcagcg tgctcgagcc
      601 cgacgcgcgg cgcgacggca ccatcggcgt cgtgagccag agcggcggcc tcggcggcat
      661 caacgtgatg tggcgggcga tggaagcggg gctgcgcatc aattactcga ccagcagcgg
      721 caacgaggcc gatctcgacg cgatcgactt tgcgggctac atgatcgagg ccccatccac
      781 cgaggtggtc ctgatggccc tcgaggcggt gcgggacggg gccaagttcg cgcgcatggc
      841 ggagcgcgca gcggaactgg agaagcccat cgtcgtcctg aagctggggc gcaccgggca
      901 cggcagccgc gcggcggcat cgcataccgg cgcgatgacc ggcgccgacg acgtcttcga
      961 ggccgcggcg cgccagttcg gcgtgaagcg cgtcaacgat tcgcgcgagc tgtacgagac
     1021 ggcgatcatg ctgcgcggca ggcgctggcc caggggccgg cgcgcggcct cgctgtcgct
     1081 ctccggcggc aacgtggtgc aggtcgccga cgtcggcgct caactggggc tcgaatggcc
     1141 cgattacacc gcgacgaccc aggccgcgct gcgcggtctg cttcccggct acggcagcgt
     1201 gggcaacccg accgacacga cttcgctggc gagcgggcag ccggaactct tccgccgcac
     1261 gctcgagctc atcgcccgcg acgggaacgt ggacgtgatg gtgccggtct tcaccgtccc
     1321 gatgcgcgcg gaactggagc tgggcgtgca gatcgcgaag gagagcgcga aaccggtggc
     1381 ggtgctgctc accgggaaat gcctggacga tccgtcgttc acggtcgagc gcatcgtggc
     1441 ggacggcgtg ccggcttacc gcgatgtcgt cacctgcctc gccgccgtgc gcgccgcggt
     1501 ggattaccgg gaattcctcg accgttttcg ccggcaggac aactactcgc ggcccgcggg
     1561 caccgattcc aaaggggcac gggcgcagct tgccggcagc atgaacgcgg gagcggggca
     1621 ggcgccgctc acggaacgcg agtccaggaa ggtgctccag tgctatggcc tgccggtgac
     1681 cgccgaccgc ctggcccgca acgccggcga agcgatcgcg catgcccgcg atatcggcgc
     1741 gcccgtcgcg ctcaagatcg aatcccccca gatcgcgcac aagacggagg cgggcgccgt
     1801 gcgcctgaac ctgtccaatc cgatggagat ccgcaccgcc tacgacgcgg tcgtcgcagc
     1861 cgcgcgccgt tacaacgcgg gcgcgacgat caacggcgtg ctggtgcagc cgatggcgcc
     1921 gcgcggcatc gagatgatgc tcggcgtcgc gaccgacccc gtcttcgggc cggtcatcgc
     1981 ggtggcgctc ggcggcatct acgcggagct gatgcgcgac atcgcctacc gggtcgcgcc
     2041 ggtcgacgcc gctcaggcgc tggccatgct gcgcgagctg cgcgcgtacc ggctgctgga
     2101 aggcgtgcgc ggccagccgc ggagcgacat cgaaacactg accgactgca tcgtgcggct
     2161 gtcgtggctc gcgcatgacc tgcgcgacgc ggtcaccgat gtcgacgtca acccgttgct
     2221 cgtgttcgag cgcggcgcgc tggcgatcga cgccctggtc gtccgccgct agcggacagc
     2281 tgaagtcgaa agtcgaaagt aaaagcgtcg accagttttg ttggcgaact ggctgtcgta
     2341 cactttcgcc tttcgccttt cgacttagga aaagctagcg ccccttgaac ttcggcgcgc
     2401 gcttttcctt gtacgccgcg atcgcctcgc gatagtcgtc ggtggactgc acgacggcca
     2461 tgtgcgagga cacgagatcg aggctcgtgc gcaggtcggt gcgcagcgac tcgtacagga
     2521 gcttcttgat caggcgctgc tggatgggtg gcccggcggc gaggcgcgcc gcgaaggcct
     2581 gcgtttccgg catcagccgg tcgtcttcgt agacgcggct cacgagcccg atgcgcagcg
     2641 cctcgttggc cttgatgaac tcaccggtcc acagcagttc cagcgctgcg gccgtgccga
     2701 cgatccgcgg gaggtaatag gcgccgccgt tgccggggag cagcccgacg cggatatacg
     2761 cctcggaaaa ccgcgccgag cgcgccgcga agcgcaggtc gcacatcagc gcgagatcca
     2821 tgccggcgcc ggccgccggc ccgttcaccg cggcgaggag cggcttgtcg aacgcttcgg
     2881 ctgcgagagc ggcctggtgg gtgacctgct gcagccgggt cttgcgctgc aggggtgtgg
     2941 gcgtggcgtt ttcgccttcc ttcgagcggc ggccgaggtc gccgccggag cagaatgcgt
     3001 cgccggcgcc ggtcaagatg aacacgcgca ccgccggatc gtcctgcgct tcccgcagcg
     3061 cgtgcgcaag cccgtccacc atgtcgtcgc tcagcgcgtt cttcttcgcc gggcgattca
     3121 ggcgcgcggt cgcgacgccg tccttgaccg tgtattcgag gtcgctcact ggggttcctt
     3181 cggaaagagg gtgattacgg gttttcgact ttcgcctttc gcctttcgcc ttcaggtttc
     3241 ctcggcctgc cgcgcccgcg gtggcggggc gtggccgccg gcgcgacggc gtaggcggga
     3301 accggctcgc ggacgtcgag cgtgaagggg ctgcgcaccg tgacgccgcc gtaagccgcg
     3361 ccatcctgca ggtcctccgt cagcagcagc gcgcagccct gcaactgcgc cgccgcgacg
     3421 atcatgctgt cccaccacga caggcgccag cgctgctcga tctcccgcgc gcgggcgagc
     3481 agcgtcgcgt caacgagatg cgggttccag tcgagaaggt ttctggtgtc atcccacgcc
     3541 gattcgggcg ctacccgcgg caacagtttg cgggtgacgt tgtagtaata ctccgacaac
     3601 acctgcatgc tcgtgcggcc ggcccggtca cgccacaagt tggcgaccca atcttccgcc
     3661 cgtgcctgct tggcggcatc cctggggtcg cgcgcgtaaa caaaaacgtt ggtgtcgacg
     3721 aaaacgcggg cgttcatgct cgtcacttct tcggccgaag cgcgtcgcga tcgtgcaact
     3781 cctcgcgcgt ggcgtaacgc tgcggcgggc ccgtaagctc cttgaagggg cctcgcgaca
     3841 ggtagcttcg catggcttcc tgataacggt ccgattcgcg gatatccttg cgcaacagct
     3901 cgccgatgta gcgcgacacg ctcatgtcgc gctccgcggc gcgcacgcgc gccaggtgcg
     3961 cggtctcctt gtcgagggtg atggtgatgt tcttcatcgg ccggcttccc gcgtgaattc
     4021 acacgcggat aataatacac ggaattcgtg tatttattgc tgccgggagg cgatgtgttt
     4081 ttagaggggt ctgacccctg ccaggtcctg ccaggtcact gcttcggcat cccggcgtcc
     4141 tgggcgacct tggcccactt ggccagctcg gccgggatca tcttgcggat ttgggcgggc
     4201 gtccggatgt cgacctccgc gctgtcgacg gcaaaccgct tggcggtttc gggcatcgcg
     4261 agtatcgccg cgatctcctt gttcagcttg ttgatgacgg cttgcggcgt gccggccgtc
     4321 gtcgcccagg cccaccagat ttccgcctgg taactcggca atcccgcctc gacggacgtg
     4381 ggcacgtcgg gcaggctcga cgcgcgcttc gtggcgccga ccgccagcgc cttgatccgg
     4441 cccgagcgga tatgggcatt cgacgtcggg atcgtcgcca cggccatgtg cgcctggccc
     4501 gacatcacgt cgagcagcgc cggggcgccg cccttgtaga gcgcgatctc gcccttgaac
     4561 ccggcatggc tgcgaaacat ggccgatacg aagtgcatga atccgcccgc cgaggccatc
     4621 gtgatgtagt tcggcttgga tttggccagc gcgaggagct cctgcagcga attgaccttc
     4681 gcggccggcc ccacggtgat cacgacgggc gccttgccga acgtcgccac ccagtcgaag
     4741 ttcctgatcg ggtcgtacgg gagcttcttg atcaccgccg ggttcatcac gaacgcggtc
     4801 gaagccatga gcagggtgta gccgtcgggg ttggcgcgcg cgacgatctc ggtgccgatc
     4861 gtgccgtcgg cgccggggcg gttgtcgacc accacctggc ggccgacgcg ctcggagagg
     4921 gcgcccccga tatagcgggc catgatgtcg ttgctgccgc cgggcgcctg cggcacgatg
     4981 acgcgaatcg gcctcacggg atagtcctgc gccgcctggg cgcacgtgat gaacgcaagc
     5041 accgagacca tcgtcatcgc gagtttcatt ttcatcggtt cctcccagtg aattggatgg
     5101 ggtcggcccc cgtttgaggt cagcccccga cgccgcccct gcggcgggcg gggatcacgc
     5161 ggccgctctt tcgcgcgggg cgaccgccgc gcggcgcgag agcatggcgg cggcgttgcc
     5221 ttcgagaatc cggtggcgtt cttcctcgcc gatattcgcc tcatcgagcg ccttccgggt
     5281 ccagtcgcag ccgaactcgg tgccgtcggt gccgcacacg acgcgctcgg cgccgtagag
     5341 acgcaccccg gcctcgatcg cccgcggccc gaacgagttg caatccacgt acacgcgcgc
     5401 gcgccggaag cgcgccgacg gcaactcttc gtccggcgtg tcgagcaggc tgcggtggtc
     5461 catgcgctcg acctcgaagg cgatgttgcc gccgaggttg tgcaggtaaa tcgccgcgtc
     5521 gggatagggc gcgagaatgt cggtgaggca cagcgtcatc atggccgagg acaggctcgc
     5581 ctgcatgtcg agcgtgccgt tgcggcggcg catgttgtcg gtgtcgccca cgtgcctggg
     5641 gaaggggtcg cccggcagcg ggccgtgatg gatgaacagg agcgcgtggt gccggttcgc
     5701 gacctcgagc agggggcgca tcttttccgc gtccttgcgc gtgaggaacc cgttccccgg
     5761 aatttgcgcg ccgaccatgc ccggcagtcc caacgcccgg tcgagctccg cggcggcgac
     5821 ggcaatgtcc gtgagcggca gcgcggcgtg ggcgaggaag cggcccttgt gcgcctggca
     5881 gatgccggag agggtgtcgt tgacgagccg gcacaggggc acggacactt ccagcggctg
     5941 cgcctcgatc cagcagaacg agccgagcag cgacagcacg ctggtggaga cgccctgccg
     6001 gtccatccgc ccgagatgga acgcaacgtc gtcgaacgcc ctggacagcg gctcctcgcc
     6061 gtagcgggac gacttcagca cttccacgcc gtcggggttg cgttcgatgc gcggattgcg
     6121 ggtgcgggcg cgcagcgcgt cggccagttc ggcgggcctc cagtgggcat gcatgtcgat
     6181 catggcagcg gcctccttag agaatcctgt tccgggcaat ctagtccaat gggccgggca
     6241 gggcaagcgc cgcgccccga aggcgaaagg tgaaaggcgg aaggcggaag gaaaaaagag
     6301 tacgtaacaa tcgattccgc cgctactttc gacttccgac tttcgacttt cgactgggtt
     6361 cttcggtcgg ccacaccggt tcattgtggc cgagcggcac catgggccgg gcccaccgcg
     6421 gcg
//
LOCUS       DBNGSG010000396         3203 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2674327, whole genome shotgun sequence.
ACCESSION   DBNGSG010000396 DBNGSG010000000
VERSION     DBNGSG010000396.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2674327"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="AC158Q_10270"
     CDS             complement(<1..228)
                     /locus_tag="AC158Q_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3340200.1"
                     /translation="MGRPAARTRQSGATTLTFLGAAEEVTGSCYLVETGGTRFLIECG
                     LFQGGRMAERKNRRFAFDPRDIAFVLLSHAHI"
     gene            complement(249..548)
                     /locus_tag="AC158Q_10275"
     CDS             complement(249..548)
                     /locus_tag="AC158Q_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3340201.1"
                     /translation="MKAAWTWLGLAGAVLLGLPAPSAGQEIPSLERGRALYENHCMVC
                     HTAKVHRRVPPLPISTDDLRSIVRIWAQEQKLRWSREEIEDVVLYLDRVHYRFEK"
     gene            complement(545..784)
                     /locus_tag="AC158Q_10280"
     CDS             complement(545..784)
                     /locus_tag="AC158Q_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340202.1"
                     /translation="MIAPRIRRRGSIRSRPSAAPAAAIEVDEEQRRHLIECCAFFRAQ
                     RFRDSGPKQYRKQDLRAAAAEIDSVIRPARRRKKP"
     gene            complement(796..1218)
                     /locus_tag="AC158Q_10285"
     CDS             complement(796..1218)
                     /locus_tag="AC158Q_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR3340203.1"
                     /translation="MANITRFDPFNDMDDLFKGLFVRPMRVDLETPQMRMKMDVTRTD
                     DTYTAKAEIPGVRKDDIQVSIDGNEVTISGEIKKESEQKKGEEVVRSERYYGKVSRSF
                     TLPHEVDEPRVTAKYADGVLNLTLPMKAKSASRKVVVN"
     gene            complement(1405..1887)
                     /locus_tag="AC158Q_10290"
     CDS             complement(1405..1887)
                     /locus_tag="AC158Q_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HIR3340204.1"
                     /translation="MRTIGTAAMIAALAAGMSTGVQAQDATYRKDIKPLVDARCAGCH
                     GTGAPTLAEFQLDQKKYTASMKGPRIDTYADLLALIGWPDTGAVMRRLDDGKSEHAGG
                     KPGNMYQFLGSTEEERQKNLGAFKAWVGPDAWVLNRFRARGSVPGITKEQLEKIRVKY
                     "
     gene            2229..2771
                     /locus_tag="AC158Q_10295"
     CDS             2229..2771
                     /locus_tag="AC158Q_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340205.1"
                     /translation="MNARTVVAAAVAAAMMTMTVSLFAAETPPATTARKSEPIYGYGM
                     MGNDERNNYRKRMREARSEQERQSIRDEHRKIMEARAKERGVTLPEPRGPRGGGPGAG
                     PGPRSEGKGPGAGKGPGQGPVVQGKGMGPGYGPGAGMGPGAGKGPGYGPGSRAECPAG
                     QDCPGPGAGKGPGYGPGLQK"
     gene            2803..>3203
                     /locus_tag="AC158Q_10300"
     CDS             2803..>3203
                     /locus_tag="AC158Q_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006402615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3340206.1"
                     /translation="MFRNILVPTDGSVLSRKAISGAVRLAKEQKARVTALFVSPSYNP
                     RVSDDSAVVNVLSPRDYDTRARAIARKNLKPVEKAAAAAGVPCDALHAYNDFPYEEIV
                     RAASRGKCDLIYMASHGRRGISRLLLGSETS"
BASE COUNT          543 a         1053 c         1025 g          582 t
ORIGIN      
        1 gatgtgcgcg tgcgacaaca gcacgaaggc aatatcgcgc gggtcgaagg cgaagcggcg
       61 gttcttgcgc tccgccattc ttccgccctg aaacagcccg cactcgatga gaaaccgcgt
      121 gccgccagtc tcgacgagat agcaggaccc ggtgacttcc tccgcggcgc cgagaaaagt
      181 cagtgttgtc gccccgcttt gccgggtccg cgcagccggt cgacccatgg ctcgcgcgcg
      241 agccgcgcct atttctcgaa ccggtagtgc acgcggtcga gatagaggac aacgtcttcg
      301 atttcctcgc ggctccagcg cagtttctgc tcttgcgccc atatcctgac gatggagcgc
      361 aggtcgtcgg tgctgatggg cagcggcggc acgcgccgat gcaccttggc ggtgtggcac
      421 accatgcagt gattctcgta gagcgcccgg ccgcgctcca ggctggggat ctcctggcct
      481 gccgacggcg ccggcagccc gagcaggacc gcacccgcca gtcccagcca ggtccaggca
      541 gctttcatgg tttctttctt ctgcgcgccg gcctgatcac cgaatcgatc tccgccgctg
      601 ccgcgcgcag atcctgcttc cggtactgct ttgggccgga atcgcgaaaa cgctgcgcgc
      661 ggaaaaaagc gcagcactcg atcagatgcc ggcgctgctc ttcgtcgact tctatcgccg
      721 cggccggcgc agccgagggt cttgagcgga tgctgccgcg ccggcggata cgaggcgcaa
      781 tcatcgccgc tcctttcagt tgacgacgac cttgcggctg gccgacttcg ccttcatggg
      841 caacgtgaga ttgagcacgc cgtcggcgta cttggccgtc accctgggtt catcgacttc
      901 gtgcgggagc gtaaaactgc gcgagacctt gccgtagtag cgctcgctgc gaaccacttc
      961 ttcgcccttc ttctgctcgc tctccttctt gatctcgccg cttatcgtta cctcgttgcc
     1021 gtcgatcgag acctggatgt cgtccttcct gaccccgggt atctccgcct tcgcggtata
     1081 agtatcgtcg gtccgggtga cgtccatctt catgcgcatc tggggtgttt ccaggtcgac
     1141 gcgcatcggg cggacgaaaa gacccttgaa cagatcgtcc atgtcgttga acgggtcgaa
     1201 acgggtgata tttgccatgg ctgtctcctg attgatgtcc caacggcctg gtcaaggccg
     1261 cacctggcgg gcaacgcccg ccttcgttcc atgctattac gcgcggctgc cgggcacttg
     1321 atctgcatca acccgacccg ccgtttgccg gggaaaaaac cgggcaaaga ccgtcccggg
     1381 ggcggccccg gcgcggtccc attctcagta cttgacccgg atcttttcca gctgctcctt
     1441 ggtaatcccg ggcacgctgc cccgcgccct gaagcggttg agtacccagg cgtctggacc
     1501 gacccatgcc ttgaacgcgc cgagattctt ctggcgctct tcctccgtgg agccgaggaa
     1561 ctggtacatg ttgccgggct tgccgcccgc gtgctcgctc ttgccatcgt cgaggcggcg
     1621 catgaccgcc ccggtgtccg gccagccgat cagcgcgagc agatcggcgt aggtatcgat
     1681 gcgcggaccc ttcatcgagg ccgtgtactt tttctggtcc agctggaatt ccgcaagcgt
     1741 gggcgccccc gtgccgtgac agcccgcgca ccgggcgtcc acgaggggct tgatgtcctt
     1801 gcggtatgtc gcgtcctgcg cctgaacgcc ggtgctcatt ccggcggcca gcgcagcaat
     1861 catagctgcc gttccgatcg ttctcatttt cagatccttt tatgaggaat gcctcgtcat
     1921 aacctgccgc ttccctgccg agacaacccc tatccgtgtc tcccgggaat tggtcgaatg
     1981 atagaagacg ggctggaggc cggtcttgat ccatgtcaat ccagggccat gcccggtgcc
     2041 atcacctgcc tgcttcacgg gcgcaatcgg gaagccctgt gttttcctct cctcgaacgt
     2101 ccgtgcggac atgaattgat ctgaatcaat acgcgcggca gaggccggca tacagttgcc
     2161 tcgtgggcgc gttccccggc tcccgggaaa cgcgaatggc gatatgcaac cggaacaagg
     2221 agcttggcat gaatgcacgc actgtagtgg ccgcggcggt cgccgccgcg atgatgacga
     2281 tgacggtctc gctgtttgcg gcagagacgc cgccggcgac caccgcccgc aagagcgagc
     2341 cgatctacgg ctacggaatg atgggcaacg acgagcgcaa caattaccgc aagaggatgc
     2401 gcgaggcgag gagcgagcag gagcgccagt ccatccgcga cgagcaccgc aagataatgg
     2461 aagcgcgcgc caaggagcgc ggcgtgacgc tgccggagcc gcgcggtccg cgtggcggcg
     2521 ggccgggtgc aggccccgga ccgcggagtg aaggcaaggg ccccggcgcc ggaaaaggcc
     2581 cgggtcaggg accggtagtt cagggcaaag ggatggggcc gggttatggt ccgggtgccg
     2641 gcatgggacc cggcgccggc aaagggccgg gctacgggcc tggctctcgc gcggaatgtc
     2701 cggccgggca ggactgtccg ggacccggcg ccggcaaagg gccgggatac ggccccgggc
     2761 tgcaaaagta accgcttttt tcaaacagga aggaaacact ccatgttcag aaatatcctg
     2821 gtaccgaccg acggctccgt gctctcgcgc aaggcgatct cgggcgcggt gcggctcgcg
     2881 aaggagcaga aggcccgcgt gactgccctc ttcgtctccc cgtcctacaa cccccgggta
     2941 agcgatgact ccgcggtagt caatgtactc tcgccgcggg actacgacac ccgggcgcgg
     3001 gcgatcgcgc ggaagaacct gaagccggtc gaaaaggcgg ccgcggccgc cggggtgccg
     3061 tgcgacgcgc tgcacgccta caacgatttc ccctacgagg agatcgtgcg cgcggcgagc
     3121 cgcgggaaat gcgatctcat ctacatggcc tcccacggcc gccgcggcat ctcccgcctg
     3181 ctgctcggca gcgagacgag caa
//
LOCUS       DBNGSG010000397         2278 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1995647, whole genome shotgun sequence.
ACCESSION   DBNGSG010000397 DBNGSG010000000
VERSION     DBNGSG010000397.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1995647"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="AC158Q_10305"
     CDS             <1..552
                     /locus_tag="AC158Q_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR3340207.1"
                     /translation="LLIAGFVLLILTSPYRLQRVIGFMDPWADAYGRGYQLSHALIAF
                     GRGEWLGVGLGGSVEKLFYLPEAHTDFLLAVIAEELGFVGVAAIIAAFAWIVARAFVI
                     GRRAVLHERYFSALVAQGIGLWVGVQAIINIGVNTGMLPTKGLTLPLVSFGGTALVAT
                     CCALAVLLRVDWENRQLAKGFTV"
     gene            549..1619
                     /gene="murG"
                     /locus_tag="AC158Q_10310"
     CDS             549..1619
                     /gene="murG"
                     /locus_tag="AC158Q_10310"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HIR3340208.1"
                     /translation="MSRTIMIMAGGTGGHIFPALAVAEHLRAQGWNAVWLGVRGGMEE
                     RLIPPRGYTMAWVRFAGVRGRGLLAAALLPLNLLVAFWQSARAIFAHRPDVVLGMGSY
                     VSFPGAMMASLLNRPLAIHEQNSVAGLANRVLAKLADRVLTTYPNAFGESTAVIWTGN
                     PVRQEIVAMAPPENRYSARAGQLRVLVVGGRPGAQVLNTVMPEALALLAPAARPRVVH
                     QAGAQHDAAVRARYQERGVAADVIAFIDDMAARYAETDLIVCRAGATTVAEIAAAGIA
                     SLLVPYPHSVDDHQTINARFLADRGAALLIPQSELNAQRLAAALGGFTRERLLAMAQA
                     ARAAGRPEATRSVAQACMELAA"
     gene            1616..>2278
                     /locus_tag="AC158Q_10315"
     CDS             1616..>2278
                     /locus_tag="AC158Q_10315"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIR3340209.1"
                     /translation="MRHKVKHVHFVGIGGAGMSGIAEVLLTLRYRVSGSDLAENAATR
                     RLRQLGARIHIGHSDEHIRGADAVVVSSAVKDDNPEVAAARAAKIPVVPRALMLAELM
                     RLRQGIAVAGTHGKTTTTSLVASVLAEGGLDPTFVIGGRLEAAGTHARLGAGEFIVVE
                     ADESDASFLYLQPVLAVVTNIDADHMETYGHSMDRLRQAFVDFLQRLPFYGVAVLCAD
                     DAN"
BASE COUNT          292 a          811 c          819 g          356 t
ORIGIN      
        1 ctgctcatcg cgggattcgt gctgctgatc ctcacgtcgc cctaccgcct gcagcgcgtg
       61 atcgggttca tggacccgtg ggcggatgcc tacggccggg gctaccagct ctcgcacgcc
      121 ctcatcgcgt tcggccgggg cgagtggctc ggcgtgggcc tgggcggcag cgtcgagaag
      181 ctgttctacc tccccgaggc tcacaccgat ttcctgctcg ccgtgatcgc tgaggagctg
      241 ggcttcgtcg gcgtcgccgc gatcatcgcc gccttcgcct ggatcgtggc ccgggcgttc
      301 gtgatcggcc ggcgcgccgt gctccacgag cgctatttct cggcgctggt ggcgcagggc
      361 atcggcctgt gggtcggggt gcaggcgatc atcaacatcg gcgtcaacac cggcatgctg
      421 cccaccaagg gcctgacgct gccgctcgtg tccttcggcg gcacggcact ggtggcgacc
      481 tgctgcgcgc tcgcggtcct gctgcgcgtg gactgggaga accgccagtt ggcgaaaggc
      541 ttcaccgtat gagccggacc atcatgatca tggccggcgg cacgggaggg catatcttcc
      601 cggcgctggc ggtggccgag cacctgcgcg ctcagggctg gaacgcggtg tggctcggcg
      661 tgcgcggcgg catggaggag cggctgatcc cgccgcgggg ctacaccatg gcgtgggtcc
      721 gtttcgccgg ggtgcgcggc cggggcctgc tcgccgcggc gctgctgccg ctgaacctcc
      781 tcgtcgcgtt ctggcagagc gcgcgcgcga ttttcgcgca ccgccccgac gtggtgctgg
      841 ggatgggcag ctatgtctcg tttcccgggg cgatgatggc ctcgctcctc aaccggccgc
      901 tcgcgatcca cgagcagaac tcggtggcgg ggctcgccaa ccgcgtgctg gcgaagctcg
      961 cggaccgcgt gctcaccacc taccccaacg ccttcggcga gtccacggcg gtgatctgga
     1021 ccggcaaccc cgtgcgccag gagatcgtcg ccatggcgcc ccccgagaac cgctactccg
     1081 cgcgggcggg gcagctgcgg gtgctcgtcg tgggcggccg cccgggcgcc caggtgctca
     1141 acacggtcat gcccgaggcg ctggcgctgc tcgcgccggc cgcgcgtccc cgcgtcgtgc
     1201 accaggcggg cgcgcagcac gacgctgcgg tgcgggcccg ctaccaggag cgtggcgtcg
     1261 ccgccgacgt catcgccttc atcgacgaca tggcggcgcg ctacgcggag accgacctca
     1321 tcgtgtgccg cgccggcgcc accaccgtgg cggagatcgc cgcggcgggc atcgcctcgc
     1381 tgctggtgcc ctatccgcac tccgtggacg accaccagac gatcaacgcg cgcttcctcg
     1441 ccgaccgcgg cgcggcgctc ctcatcccgc agagcgagct gaatgcgcaa cggctcgccg
     1501 cggcgctcgg cggcttcacg cgcgagcggc tgctcgccat ggcgcaggcc gcgcgcgccg
     1561 ccggccggcc cgaggcgacg cgctcggtgg cgcaggcttg catggagctc gccgcatgag
     1621 acacaaggtc aagcatgtcc actttgtagg gattgggggc gcggggatga gcggcatcgc
     1681 ggaagtgctg ctgacgctcc gttaccgggt gagcggctcc gacctcgccg agaatgccgc
     1741 gacgcggcgg ctgcgccagc tcggcgcccg catccacatc ggccactcgg acgagcacat
     1801 ccggggcgct gacgccgtgg tcgtgtcgag cgcggtcaag gacgataacc ccgaggtcgc
     1861 cgccgcgcgc gccgccaaga tacccgtggt tccgcgggcg ctgatgctcg ccgagctgat
     1921 gcggctgcgc cagggcatcg cggttgccgg cacgcacggc aagaccacga ccacgagcct
     1981 cgtcgccagc gtgctcgcgg aaggcggtct cgacccgacc ttcgtgatcg gcgggcggct
     2041 cgaggcggcg ggcacccacg cccggctggg agcgggcgag ttcatcgtgg tggaggccga
     2101 cgagtccgac gcgtcgttcc tctaccttca gccggtgctg gcggtcgtca ccaatatcga
     2161 tgccgaccac atggagacct atggacactc gatggaccgc ctgcgccagg cgttcgtcga
     2221 tttcctgcaa cgcctgccgt tttacggcgt ggcggtgctg tgtgccgacg acgccaac
//
LOCUS       DBNGSG010000398         4107 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_297886, whole genome shotgun sequence.
ACCESSION   DBNGSG010000398 DBNGSG010000000
VERSION     DBNGSG010000398.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4107)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_297886"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..1424)
                     /locus_tag="AC158Q_10320"
     CDS             complement(195..1424)
                     /locus_tag="AC158Q_10320"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006493921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HIR3340210.1"
                     /translation="MNDTTTHPSETNLRVAGRRDQADVPRRFKNILCLDDFEEPARRF
                     LPRPIFGYVSGGVETNASREANRAAFGEWEFLPRILKSTRGRHQKATVLGRTYDLPFG
                     FPPMGGTSLAAYRGDSVLAQVAAELNTVMIQSGASLTPMERIREIGPTAWFQAYLPGD
                     REIIVPLVERAQRAGFDTLVLTVDVQVPANRENNVRTGYNSPLRPTARLAWDCLLRPR
                     WTAGVFGRTVLNHGMPHLENMGFPRIPILGHVERPRMARDGLAWEHLELMRKAWKGKL
                     VLKGVLAPQDVRIARESGVDGILVSNHGGRQLDYTVAPLRVLPAVAAEAGNMTIMMDS
                     GIRRGTDVLKALALGAQFVFVGRPILYAAAIAGAAGVHHAASLLREEISRDMAMLGIS
                     SLAEMKRELLVPSRGPI"
     gene            complement(1468..1920)
                     /locus_tag="AC158Q_10325"
     CDS             complement(1468..1920)
                     /locus_tag="AC158Q_10325"
                     /inference="COORDINATES: protein motif:HMM:NF013228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR3340211.1"
                     /translation="MLKRHYITPENTYKVGGNRQVIYTPVIVVEDTSHTHVYLAGRTS
                     RTLDGDVACKGDMRGQIREVCKAIQIALESVGANFADVTRTITYTTDMNAYFAAIDER
                     FKFFKPPLPTSTLIGVARLAMPEMLVEIEVEAIIETARLRLPKNAVSR"
     gene            complement(2023..2997)
                     /locus_tag="AC158Q_10330"
     CDS             complement(2023..2997)
                     /locus_tag="AC158Q_10330"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3340212.1"
                     /translation="MHNSLVKALVAAALLFSWVAAASGQAYPSRSVRVIVPYPPGGGA
                     DFVTRVFSKALGDNLGQQFVVDNRAGANGNIGSELAARSKPDGYTLLGVANTTVTINP
                     SLYRQMPIDMLRDLAPVSIMAGQPNVLIVHPSLPVKNVKDLVAIARARPGQLDYASAG
                     TGSSSHIAAELFRLIAKVNVTHVPYKGNGPAMADLIGGHVQLMFNNLAPSISQVKAGK
                     LRALAVTGEKRSTAAADVPTMAEAGYPGVVFNLWVGLLAPAGAPADVISKISAETAKA
                     AQLRDVRERLLGEGTEPYTTSPAQMADIMRKETAQWVKVVKAANIAPQ"
     gene            3375..>4107
                     /locus_tag="AC158Q_10335"
     CDS             3375..>4107
                     /locus_tag="AC158Q_10335"
                     /inference="COORDINATES: protein motif:HMM:NF022665.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3014 domain-containing protein"
                     /protein_id="HIR3340213.1"
                     /translation="MAEYDRDWPARDDPPNWREYEPERPGMSLWAWALLVVLVASVAG
                     YYFWQQRQPAPSAITRPPVVAPAPEAETPAAEKPAIRHPVPDAEAEPEKPLPGLDQSD
                     TLIGDRLAGLVGLTAFKSFVSPEQLVRRIVATVDNLPRKIAPVRMRPVRPVPGQFLPG
                     AGNRTRYAAHVKVFESLDARALVQAYVKLYPLFQGAYVELGHLDGYFNDRLVEAIDDM
                     LAAPELAGAPELVQPKVLYLYADPGL"
BASE COUNT          640 a         1339 c         1420 g          708 t
ORIGIN      
        1 cacggcgctc aggctcttgc cttccgcgcg cgcgagcgcc tccacgtcgc gcgcaagcgg
       61 cgcgggcagg gagatggttt tcttgacgac attacccatg gcggctggat gatccgtagt
      121 gtgaataaat catacccgtc attcgcagtg ccggcaaggc accagggcct gactgccgtt
      181 actcgtcacc ggagtcagat cggtccgcgg ctcggcacca gcagctcgcg cttcatctcg
      241 gccagcgatg agatgccgag catggccatg tcgcggctga tttcctcgcg caggagggag
      301 gcggcgtggt gcacgccggc cgcgcccgcg atcgccgccg cgtagaggat cggccggccg
      361 acgaagacga actgcgcacc cagggcgagc gccttcagca cgtcggtgcc gcggcggatg
      421 ccgctgtcca tcatgatggt catgttgccg gcctcggcgg cgaccgcggg cagcacgcgc
      481 agcggcgcca cggtgtagtc gagctggcgg ccgccgtggt tcgacaccag gatgccgtcc
      541 acgccgctct cgcgcgcgat gcgcacgtcc tgcggcgcga gcacgccttt cagcacgagc
      601 ttgcccttcc aggccttgcg catcagctcg aggtgctccc aggcgaggcc gtcgcgcgcc
      661 atgcgcgggc gctcgacgtg gcccaggatc gggatgcggg ggaagcccat gttctccagg
      721 tgcggcatgc cgtggttcaa cacggtgcgg ccgaacacgc cggcggtcca gcgcgggcgc
      781 agcaggcagt cccacgcgag ccgcgcggtg ggcctcaacg gcgagttgta gcctgtgcgc
      841 acgttgttct cgcggttcgc gggcacctgc acgtccaccg tgagcacgag cgtgtcgaag
      901 cccgcccgct gcgcgcgctc gacgagcggc acgatgatct cccggtcgcc gggcaggtag
      961 gcctggaacc aggcggtcgg gccgatttcc cggatacgct ccatgggcgt gagggaggcg
     1021 ccgctctgga tcatcacggt gttgagctcg gccgcgacct gcgccaggac ggagtctccc
     1081 ctgtaggcgg cgagcgacgt gccgcccatc ggcggaaagc cgaagggcag gtcgtaggtg
     1141 cgccccagga ccgtcgcctt ctggtggcgg ccgcgggtgc tcttcaggat gcgcggcagg
     1201 aattcccatt cgccgaaggc cgcgcggttg gcctcgcgcg acgcgttcgt ctccacgccc
     1261 ccggacacgt agccgaagat cgggcgcggg aggaagcggc gcgccggctc ctcgaagtcg
     1321 tcgaggcaga ggatgttctt gaaccggcgc gggacgtcgg cctggtcgcg ccggccggcg
     1381 acgcgaagat tggtctcgct ggggtgggtg gtcgtgtcgt tcattctggc tttcccggga
     1441 tcgatcgttg ccgaagcgtg ccggcgttca tcgcgaaacg gcgttcttcg gcaggcgcag
     1501 gcgcgcggtc tcgataatcg cttccacttc gatctccacc agcatttcgg gcatcgcgag
     1561 gcgcgcgacg ccgatcagcg tgctggtggg cagcggcggc ttgaagaact tgaagcgctc
     1621 gtcgatcgcc gcgaaataag cgttcatgtc ggtcgtgtag gtgatggtcc gcgtaacatc
     1681 cgcgaagttg gcgccgacgg attcgagcgc aatctggatg gccttgcaga cctcacggat
     1741 ctgcccgcgc atgtcgccct tgcacgcgac gtcgccgtcg agcgtgcgcg aagtgcgccc
     1801 ggccaggtag acgtgggtgt gcgaggtgtc ctcgaccacg atgacgggcg tatagatgac
     1861 ctgcctgttg ccgccgacct tgtaggtgtt ctccggcgtg atgtaatggc gcttgagcat
     1921 cgttcctcct ctttgggtga tgggtgatga gtgatgtgag ctgggaattt agaacttccc
     1981 cctgcgcggg agtcttatca cttgtcactc gtcacttgtc tcttactgcg gcgcgatgtt
     2041 cgccgccttg acgaccttga cccactgggc cgtttccttg cgcatgatgt ccgccatctg
     2101 ggccgggctc gtcgtgtacg gctcggtgcc ttctcccagc aggcgctcgc gcacgtcccg
     2161 caactgcgcc gccttggccg tctcggcgct gattttcgag atgacgtccg cgggcgcacc
     2221 ggccggcgca agcaggccca cccacaggtt gaatacgact cccgggtaac ccgcttcggc
     2281 catggtcggc acgtccgccg cggccgtcga gcgcttctcc ccggtcaccg ccagcgcgcg
     2341 cagcttcccg gccttgacct gcgaaatgga cggcgccagg ttgttgaaca tcaattgcac
     2401 gtgcccgccg atcaggtcgg ccatggcggg gccgttgccc ttgtagggca cgtgcgtgac
     2461 gttgaccttc gcaatcaggc ggaacagctc ggccgcgatg tgcgaggacg agccggtgcc
     2521 cgcggaggcg taatcgagct ggccgggacg cgcgcgcgcg atggccacca ggtccttgac
     2581 gttcttcacc ggcagggaag gatgcacgat cagcacgttc ggctgtcccg ccatgatgct
     2641 gaccggcgcc aggtcgcgca gcatgtcgat cggcatctgc cggtacaggc tggggttgat
     2701 ggtgacggtg gtgttggcga cgcccagcag cgtgtaaccg tcgggcttgg acctggccgc
     2761 aagttccgaa ccgatgttgc cgttcgcgcc ggcgcgattg tccacgacga actgctgtcc
     2821 caggttgtcg cccaatgcct tgctgaaaac gcgcgtcacg aaatctgcgc cgccgccggg
     2881 cgggtagggg acgatcaccc gcaccgaccg cgacgggtag gcctggccgg acgcggccgc
     2941 gacccacgag aacagcagtg ccgcggccac tagtgccttc accaaactat tgtgcatggg
     3001 ccgatcctcc ggaacgattc cggcataccc taacccaacg tccggcccgc atcaatgttg
     3061 cgcccggcat ggtccacatg ctgaaggcgg aaggtggaag ggcttgaaac ccggtgacga
     3121 gtgacgagga gtcgggctca cgcccggcga agatgaagcg tcgcagatgg ggcgtcgcag
     3181 atgaggcgtc gcaggtgagg cgcgcagtac ctgagacgta gtatctgaga cgtagtacct
     3241 gagacgcagt atctgagacg cagtacaaga aagccagccg ccctcacggt ctttctgtgt
     3301 cctccgtgtc gaagctttaa aaggcggaag gcggaacgcg gaacgctcgg ccaaagccgc
     3361 gttccaatac ctatatggcc gaatacgatc gcgactggcc ggcccgggat gaccccccga
     3421 attggcggga atacgagccc gagcgcccgg gcatgtcgtt gtgggcctgg gcgctgctgg
     3481 tagtgctcgt ggcgtcggtc gcgggctatt acttctggca gcagcgccag ccggcgcctt
     3541 ccgcgataac ccggccgccc gttgtcgccc ccgctccgga agccgaaacg ccggccgcag
     3601 agaagccggc gatacgccac ccggtcccgg acgcggaagc cgagccggag aagccgctgc
     3661 cggggctcga ccagagcgat acgctgatcg gcgaccggct cgcaggcctg gtcggactga
     3721 ctgcgttcaa gtccttcgtg agcccggagc agctcgtgcg ccgcatcgtc gccaccgtcg
     3781 acaatctacc gcgcaagatc gcgccggtgc gcatgcggcc ggtgcggccg gtgccgggcc
     3841 agttcctgcc gggcgccggc aaccggacgc gctatgccgc ccacgtgaag gtgttcgaat
     3901 cgctcgatgc acgcgcgctc gtgcaggcct acgtgaagct gtatcccttg ttccagggcg
     3961 cctacgtgga gctggggcat ctggacggtt atttcaacga tcgcctggtg gaggcgatcg
     4021 acgacatgct cgccgcgccg gagctggcgg gcgcgccgga gctggtgcaa ccgaaggtgc
     4081 tgtacctcta tgccgacccc ggcctcg
//
LOCUS       DBNGSG010000399         2858 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1486189, whole genome shotgun sequence.
ACCESSION   DBNGSG010000399 DBNGSG010000000
VERSION     DBNGSG010000399.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1486189"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1499)
                     /locus_tag="AC158Q_10340"
     CDS             complement(<1..1499)
                     /locus_tag="AC158Q_10340"
                     /inference="COORDINATES: protein motif:HMM:NF018513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HIR3340214.1"
                     /translation="MSETAAPVTRPRPALEWLYYLTAIFFFVYLFVYYWTGEGGPTLL
                     AFALVPVTFILFTLEALRKNEFYPRLPALANYAIAAIYIAISVAVAIYMVVEYDEIGT
                     VRAGAWNAADLALGAVMVVLVMEYARQRYLALFILNVILILYAVYGQVVPGMFNHPGL
                     SWERIVTAMSVETSTGIYSNLSQLALTLIGSFILVLSALRAFGCVESILRASKQVAMR
                     SPHALPQSAVFGSMAVGTVSGSGAANAITVGSATIPAMIGAGMPRVVAAGIETASSLG
                     GQLMPPVMGISAFLMAEFLGRSYFDVVARGYAPALIYYAGVSVSVYLLSTRYRARLVT
                     TTPEPMQWSDWMNIAAFVVVVGGLITLMATLHLAPMFAALYIFLFVAAFLVVAHLVVL
                     ALTQGWSWRAVIAPLGRLVDNFASMTSDLTLLLAMLSIMTGAFVITGVPTKVGALLIE
                     AAGVNIATMAIVAFFFGALLGTGLPPAPTYIITALVIVPPMIKVGVDPWV"
     gene            complement(1587..2606)
                     /locus_tag="AC158Q_10345"
     CDS             complement(1587..2606)
                     /locus_tag="AC158Q_10345"
                     /inference="COORDINATES: protein motif:HMM:NF028178.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HIR3340215.1"
                     /translation="MFKRTLALLAAGLLVALAAPAWSQVKEIPWGTSSVGSAGHKALA
                     ILADVLNREMPKYRVTVQPTPGAIVTVKGYSTGQFEGYYGADIAFYEMANDIKRFKGF
                     KSSMKRQPVQSFWTYTVEMGMAVHARDKGKFKGWNDLAGKALFTGNPPWDTRAQLERA
                     FEALGIKYQYRQVDLSAAGSLLESGRIEGFCLYSNGESGVPPWITEASLATDWAAINP
                     SAAEVAALRKAGFAILEQKPEVFKRDVHVDKVILLPFYYGFHVGLEVPEDDMYQMLKV
                     IEKNLPALVKADASFAQIAKDMPGFQRRGVTSSANLLPIHPGLAKYMREKGVWDAKWD
                     ARIAK"
BASE COUNT          529 a          871 c          992 g          466 t
ORIGIN      
        1 acccacggat cgacgccgac cttgatcatg ggcggcacga tcaccagcgc ggtgatgatg
       61 taggtcggcg cgggcggcag cccggtgccg agcagcgcgc cgaagaagaa ggcgacgatc
      121 gccatggtgg cgatattcac gcccgccgcc tcgatgagga gggcaccgac cttggtcggc
      181 acgccggtga tgacgaacgc gccggtcatg atggacagca tcgcgagcag cagggtcagg
      241 tcggaggtca tgctcgcgaa gttgtcgacc aggcgcccga gcggcgcgat gaccgcgcgc
      301 catgaccagc cctgagtgag cgcgagcacg accaggtgtg cgacgaccag gaacgccgcc
      361 acgaaaagga atatgtatag cgcggcgaac atgggcgcca ggtgcagcgt tgccatgagc
      421 gtgatcagtc cgcccacgac gacaacgaag gcggcgatgt tcatccagtc cgaccactgc
      481 atcggctccg gggtggtggt cacaagtcgg gcgcgatagc gcgtggacag caggtagacg
      541 gagacgctga cgccggcgta atagatgagc gcgggggcgt agccgcgcgc gacgacgtcg
      601 aaatagctgc ggccgaggaa ttccgccatg agaaatgcgg agatgcccat gaccgggggc
      661 atcagctggc ctccgaggga ggaggcggtc tcgataccgg ccgcgaccac tcgcggcatg
      721 ccggcgccga tcatggccgg aatcgtagcc gagcccacgg tgatcgcgtt ggccgcgccg
      781 ctgccgctca ccgtgccgac cgccatggag ccgaatacgg cggactgcgg cagcgcgtgc
      841 ggcgagcgca tcgccacctg ctttgacgcc ctcaggatcg actcgacgca gccaaaggcg
      901 cgcagcgcgg acagcacgag gatgaacgag ccgatcagcg tcagcgcgag ctgcgacagg
      961 ttggagtaaa tgccggtcga ggtctcgacg ctcatcgcgg tcacgatgcg ctcccaggag
     1021 agcccgggat ggttgaacat gcccggcacc acctggccgt aaaccgcgta gagaatcagg
     1081 atgacgttca ggatgaacag cgccaggtag cgctggcggg cgtactccat gaccagaaca
     1141 accatcacgg cgccgagggc gagatcggcc gcgttccagg ccccggcgcg caccgtgccg
     1201 atttcgtcgt actcgacgac catgtagatc gccacggcga ccgagatggc gatgtaaatc
     1261 gcggcaatgg cgtaattggc gagggccggg agccgcgggt agaactcgtt cttgcgcagc
     1321 gcctccagcg tgaacaggat gaacgtgacc gggaccagcg caaaggcgag cagggtcggg
     1381 ccgccctcgc cggtccagta atagacgaag agatagacga agaaaaagat cgccgtcagg
     1441 tagtagagcc actcgagggc cgggcgtggc cgggtgactg gcgcagcggt ctcgctcatg
     1501 gggaatcagc cggcacgcgc ggccgttcgg ggagggcggt cgcccgcccg tccccggatg
     1561 cattcaagcc gccggctcaa cttgctttac ttcgcgatcc tggcgtccca cttcgcgtcc
     1621 cagacgccct tctcgcgcat gtatttcgcg aggcccgggt ggatcggcag gaggtttgcc
     1681 gaggacgtga ccccgcggcg ctggaatccg ggcatgtcct tggcgatctg ggcgaacgag
     1741 gcgtcggcct tgaccagcgc gggcaggttc ttttcgatca ccttgagcat ctggtacatg
     1801 tcgtcctcgg ggacttcgag gccgacgtgg aagccgtagt agaacggcag caggatcacc
     1861 ttgtcgacat gcacgtcgcg cttgaagacc tcgggcttct gctcgaggat cgcgaagccg
     1921 gctttccgga gggcggccac ttcggccgcg ctcggattga tggcggccca atccgtcgcg
     1981 agcgaggcct cggtgatcca gggcggaacg ccggactcgc cgttgctgta cagacagaac
     2041 ccctcgatgc ggccgctttc gagcagcgag ccggcggcgg acaggtcgac ctggcggtat
     2101 tggtacttga tgccgagcgc ctcgaacgcg cgttcgagct gggcgcgcgt gtcccacggg
     2161 gggttcccgg tgaagagcgc cttgccggcc aggtcattcc agcccttgaa cttgcccttg
     2221 tcgcgcgcat gcaccgccat gcccatctcg acggtgtagg tccagaagga ctggaccggc
     2281 tggcgcttca tgctcgactt gaagcccttg aagcgcttga tgtcgttggc catctcgtag
     2341 aacgcgatgt cggcgccgta gtagccctcg aactggccgg tcgagtagcc cttcacggtc
     2401 acgatcgcgc cgggcgtcgg ctgcaccgtc acccggtact tcggcatttc gcggttgagc
     2461 acgtccgcga gaatggcgag cgccttgtgc cccgcggacc cgacggaaga cgtgccccac
     2521 gggatttcct tgacctggga ccaggccggg gcggccaggg ccaccaacag gccggcggcc
     2581 agcagggcga gtgtgcgctt gaacatggtt gtatcctcct ttgtgagcgc ggaggattct
     2641 gaggatcctc ctgcgggccc attgtattac aaccttttaa gtgtcgggtg acgagtgacg
     2701 agtgtcgggt gacgggtgac gggtgacggg gaaagtgtga gaagtgatgg ttaatgaagg
     2761 ggttttcttc gcatccctca tccctccacc atcagaagga cgaccccggc tgcttcagaa
     2821 acttcttttc cgcgggagtg gattcgcgtc cgagtatc
//
LOCUS       DBNGSG010000400         5325 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4033632, whole genome shotgun sequence.
ACCESSION   DBNGSG010000400 DBNGSG010000000
VERSION     DBNGSG010000400.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5325)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5325)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5325
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4033632"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..1169)
                     /locus_tag="AC158Q_10350"
     CDS             complement(198..1169)
                     /locus_tag="AC158Q_10350"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3340216.1"
                     /translation="MKNAILLFLVSILSCAAPGVAAADAAAGYPGRPIRFITPFVAGG
                     PSDTLSRLLGQKLTESWNQTIVIDNRGSAGGIVGFDLAAKSDPDGYTLLLANGAGLTI
                     NPHVYLKLPYDPTRDFQPITQITSGAYYMVVPVSLPARTMKEYIALAKAKPGTLNFAA
                     TGTNNLLAAEQLNYMAGIKAVAVNYKGTGQAVSAVMSGEVQSFIMSPLIAIPQVKAGK
                     LRAIGITGPKRSPALPEVPTIAETLPGYENITWHSVVVPARTPRPIVAKLSKELMRII
                     RQPDVQERFNSVGLDSVGSTPEELAALIKKEVVMYGKLVKQIGLKPQ"
     gene            1515..1763
                     /locus_tag="AC158Q_10355"
     CDS             1515..1763
                     /locus_tag="AC158Q_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340217.1"
                     /translation="MSGLDLGNLRPFLDEQEIFAASIEPDDPRAAVAKRMLGIILEAR
                     GNLRPSTDSTANYVPNKQIVEELIALRYEADLIGNRKK"
     gene            complement(1843..2475)
                     /locus_tag="AC158Q_10360"
     CDS             complement(1843..2475)
                     /locus_tag="AC158Q_10360"
                     /inference="COORDINATES: protein motif:HMM:NF014025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HIR3340218.1"
                     /translation="MLDWTEYAKMLLALLVIVNPVGAVPLFASLTSENTAAEKHHIAR
                     IASTTVAVVLIVAAVGGQPLLALFGITIASFKVGGAILILLIAISMMHGTQSGEKQTP
                     EEAREAAYKERIAVVPLAIPLLSGPGAISTIIIYATAQSSPAHLGAVVACALLVALIT
                     WIALRVATPVSQWFGKTGVNIAMRLMGLLLAAVAVEIFASGIVVLLPGLS"
     gene            2548..3684
                     /locus_tag="AC158Q_10365"
     CDS             2548..3684
                     /locus_tag="AC158Q_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340219.1"
                     /translation="MKAIGVAALFFLGLSSGAALARDLQVEMEVLHIGGRLAPAELDA
                     LLADERLTIEAGYQPTRLIEGDTARRERTMPFGSRLFAISRQLELQGAQVERSGRTLR
                     FRVADVPADHDGYRLNSLVLSIPVAPGPGRPQPGLSLNLLNPVPRSGAQETAFLTRHG
                     AFDLGLRVSYRWSDARGAAAPAPTVCNGDVQPAGAGQYRFRPRHPLAGLMRFLASDVQ
                     SDPPSRPVAGRESLRMREPFPEPLTGWKLSRNHLLRLQVDGQSVERLSVYAEQAGPGH
                     CRRARLYEALFAGGRAVTLQRSVNENECGAGDPDSRSVTADWLEDGSLARFLTSTNKG
                     SQTWDGFAATADPACGPGAAPPSPGEVQALIAEMQRLRDAFVRR"
     gene            3777..4067
                     /locus_tag="AC158Q_10370"
     CDS             3777..4067
                     /locus_tag="AC158Q_10370"
                     /inference="COORDINATES: protein motif:HMM:NF052504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sll1863 family stress response protein"
                     /protein_id="HIR3340220.1"
                     /translation="MSKRDQYVEKLKAQLDEWNAEVAKWEAKSRGAQADMRVEYEKQL
                     ETFRRQRNQALEQMRKVQAATGDAWVDLVRGADDAWAKMREAFEKARTHFHK"
     gene            complement(4152..5120)
                     /locus_tag="AC158Q_10375"
     CDS             complement(4152..5120)
                     /locus_tag="AC158Q_10375"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3340221.1"
                     /translation="MTHAARILWALVACACLAGTALAQKYPARAVRIIVPQQPGDPCD
                     TFGRLLGGYAAGRLGQQFVVDNRPGAGGRIGLGLAANATPDGYTIACGQGGNMSVIPH
                     TMKDVPYHALNDFAPVALVATNYLVLVAANKAPFKTTAELVKYLKANPGRVSFATNGE
                     GAFLHMATELFRSAVGFEYIHVPFKGIGDMASQLQSSQVDAAFSSFTAMHPHIEAGRV
                     RLLGIAKETRAHNYPNFPTVAETVPGYSSGGWFGFVAPAKVPKEVITTLNREFNAAMK
                     QPDIRAKLEALGLEVWNESPEYFGKLMKSEYDKYGKVARDIKLVPK"
BASE COUNT          952 a         1642 c         1783 g          948 t
ORIGIN      
        1 gcggccatgg gcgccgtgat cgagatcagg acggccgacg agatgcgcaa gatcatcccg
       61 gccgagatcg ccaagtggac ccaggtcgcc gtcgacgcgg gcataccgcg ggccgacaag
      121 taggccgcgg gaaatcaggg gaagagattg cggctcctcg acgctctgtg gagccgactc
      181 actcatttcg gtgcgcgcta ttgcggcttg aggccgatct gcttgaccag cttgccgtac
      241 atcacgactt ccttcttgat cagggcggca agttcctccg gcgtcgagcc caccgaatcg
      301 agccccaccg aattgaagcg ctcctggacg tccggctgcc ggatgatgcg cattaactcc
      361 ttcgagagct tggctacgat cggccgcggc gtcctggcgg gaacaaccac gctgtgccag
      421 gtgatgttct cgtagccggg cagcgtctcc gcgatagtcg gaacttccgg gagggcgggg
      481 ctgcgcttgg gccccgtaat tccgattgcc cgcaatttcc ccgccttgac ctgggggatg
      541 gcgatcagcg ggctcatgat gaacgactgc acttcgccgc tcatcaccgc gctgaccgcc
      601 tgcccggttc ccttgtaatt cacggctacc gccttgatgc cggccatgta attcaactgc
      661 tccgccgcca gcaggttgtt ggtgccggtc gccgcgaaat tgagtgttcc cggcttggct
      721 ttggcgagtg cgatgtactc cttcatggtc ctggccggga gagacaccgg gaccaccatg
      781 taatacgcgc cggaggtaat ctgcgtgatg ggctgaaagt ccctggtggg atcgtacggc
      841 agcttgagat acacatgcgg gttgatggtg agccccgccc cgtttgcgag cagcagcgtg
      901 tatccgtcgg gatcggactt ggcggccagg tcgaagccga cgatgccgcc cgcgcttccg
      961 cgattgtcga tgacgatggt ttgattccag ctttcggtca gcttctggcc gagcagccgc
     1021 gagagcgtgt cgctcgggcc gccggccacg aacggcgtga tgaagcgaat gggcctgccg
     1081 ggatatccgg ctgcggcatc cgccgcggcg acgcccggtg cggcgcatga caaaatcgaa
     1141 accaggaaca gaagaattgc atttttcatt gaagcctcct caagttggct gaaaatgccc
     1201 tgtccgcgag tccgagtaga gtacttgctt ttagtggatt agtgtgccgg gacatcggcc
     1261 tgtcctggtc gtaagagggg aacgagtcgc gagtcgaaat gggtcgtgag ggcgcgggcc
     1321 ccataaaaag aaatatagat caaaaggttg atgttgtttt ccacgcccct gcaggaattc
     1381 gtaatcctcg gcgatcaggg gctcacttcg tacttcggcc agccgcgcta aaatgaatca
     1441 agggggacag acaatcgttt tcggcgggcg gtaaacatac tccagccacc tggcggtact
     1501 gaaaggctaa ctttatgtct ggtctggacc tcggcaatct ccggcccttc ctcgacgagc
     1561 aggaaatatt cgcggcttcg atcgagccgg acgatccgcg tgccgccgtc gcgaagcgca
     1621 tgctggggat cattctcgag gcgaggggca acctgcgtcc gtccaccgat tcaacggcta
     1681 actacgtgcc gaacaagcaa atcgtcgagg agctgatcgc gctgcgttac gaagccgacc
     1741 tgatcggcaa caggaaaaag taagcttggg gccggaatca ttttcaaacg gaatattgcc
     1801 gcgttccatt tcctttcaag caagggcact tgagccgggt cgttaactca agccgggaag
     1861 caggacaacg ataccgctgg cgaagatttc cacggctacc gcggccagca gcaagcccat
     1921 cagccgcatc gcgatattga cgccggtctt gccgaaccat tggctgaccg gcgtcgcgac
     1981 ccgcagcgcg atccaggtaa tgagcgcgac cagcagggcg cacgcgacga ccgcgccgag
     2041 atgcgccggc gaggactgtg ccgtggcgta gatgatgatg gtggagatcg ccccgggccc
     2101 ggaaagcagg ggtatcgcga ggggcacgac ggcgatgcgt tccttgtaag cggcctccct
     2161 ggcctcttcc ggcgtctgtt tttccccgct ctgcgtgccg tgcatcatcg aaatggcgat
     2221 tagcagtatc aggattgcgc cgccgacctt gaacgacgcg atcgtgatgc cgaacagggc
     2281 gagcagcggc tggccgccca ccgcggcgac gatcagcacg acggcgaccg tggtgctggc
     2341 gatccgcgcg atgtggtgct tttcggccgc ggtgttttcc gaggtgaggc tggcgaacaa
     2401 gggcaccgcg ccgacgggat tgacgattac gagcagcgcc agaagcatct tggcgtattc
     2461 ggtccagtcg agcatacggc tagtttactc tccgcgcccc ggccggaact ccgggtaaac
     2521 tggctgttcc ggcccaagga agaaagcatg aaagcaatcg gggtcgctgc actgtttttt
     2581 ctgggcttga gctccggggc tgcgctggcg cgcgatctgc aagtcgagat ggaggtgctg
     2641 cacatcggcg gccggctcgc accggcggag ctcgacgccc tgctggcgga cgagcgcctg
     2701 acgatcgagg caggctacca gcccacgcgc ctcatcgaag gtgacacggc ccgccgtgag
     2761 cgcacgatgc ctttcggcag caggctgttc gccatctccc ggcagctgga actccagggc
     2821 gcgcaggtcg aacgctcggg ccgcaccttg cgtttccggg tggccgatgt tcccgcggac
     2881 cacgacggct accgcctgaa cagcctggtt ctgagcatcc cggttgcgcc gggcccggga
     2941 cgtccgcagc ccggcctgag cctgaacctg ctgaacccgg tgccccggtc gggcgcccag
     3001 gaaaccgcat tccttacccg gcacggcgcg ttcgacctgg gcttgcgcgt gagttaccgc
     3061 tggagcgatg ccaggggcgc ggcggcgcca gctccgacag tctgtaacgg ggacgtccag
     3121 ccggccggcg ccggccagta ccgctttcgc ccccggcacc cgctggccgg gctgatgaga
     3181 ttcctggcgt cggacgtgca gagcgatccg ccgtcgcgcc cggtcgcggg gcgggagagc
     3241 ctgcgaatgc gcgagccgtt tcccgagccc ttgaccggat ggaaactgtc gcgcaaccat
     3301 ctgctgcgat tgcaggtcga tggccagtcc gtcgagcggc tgtcggtgta cgccgagcaa
     3361 gccggccccg ggcattgccg ccgcgcgcgc ttgtacgaag ccttgttcgc gggcgggcgg
     3421 gcggtcacgc tgcagcgctc ggtcaatgaa aatgagtgcg gcgccgggga cccggacagc
     3481 cgcagcgtca cggccgactg gctggaggac ggcagcctgg cccgtttcct caccagcacg
     3541 aacaagggca gccagacgtg ggatggtttt gccgcgacgg ccgatcccgc ttgcggcccg
     3601 ggcgccgcgc cgccgtcgcc cggagaagtg caggcgctga tcgccgagat gcagcggcta
     3661 cgcgacgctt tcgtgcgccg gtgaccggat gaatgtcgaa ttgcggagaa taatcgctac
     3721 cctcagtctc ccgtttcatg caggagcccc aacccaacca ccttgaagga ggcaccatga
     3781 gcaagcgaga ccagtatgtc gagaaactca aggcacagct cgatgagtgg aatgccgaag
     3841 tggcgaagtg ggaagccaag tcccggggcg cgcaggccga catgcgcgtc gaatacgaaa
     3901 agcagctcga gacgttccgt cgccagcgca accaggcgct ggaacagatg cgcaaggtgc
     3961 aggcggcaac gggcgatgca tgggtcgacc tcgtgcgcgg ggcggacgac gcctgggcga
     4021 aaatgcgcga ggctttcgaa aaggcgcgca cgcattttca caagtgaatc gacggtagcg
     4081 ccggtcggat gtgcagggcc gaaaaaaaac cccgcctcgg ggggtttttt tcggagcgag
     4141 acgcgaggag gttatttcgg caccagcttg atgtcgcggg ccaccttgcc gtacttgtcg
     4201 tactcgctct tcatcagctt gccgaaatat tccggcgatt cgttccagac ctccaggccc
     4261 agcgcctcga gcttcgcgcg gatgtcgggc tgcttcatcg ccgcgttgaa ttcccggttg
     4321 agggtggtga tgacctcttt cggcaccttg gccggggcga cgaagccgaa ccagccgccg
     4381 gaggagtatc ccggcacggt ttccgcgacg gtggggaagt tggggtagtt gtgcgcgcga
     4441 gtttccttgg cgatgccgag cagccgcacc cggccggcct cgatgtgcgg atgcatggcc
     4501 gtgaacgagc tgaacgcggc atccacctgg ctggattgca gctgggacgc catgtcgccg
     4561 atgcccttga acggcacgtg gatgtactcg aagcccacgg ccgagcggaa gagttccgtc
     4621 gccatgtgca ggaacgcgcc ctcgccgttg gtcgcgaagc tcaccctgcc ggggttggcc
     4681 ttcaggtact tcaccagttc cgcggtggtc ttgaaggggg ccttgttggc ggccaccagc
     4741 accagatagt tcgtcgcgac cagcgcgacc ggcgcgaaat cgttcagcgc gtggtagggc
     4801 acgtctttca tcgtgtgcgg gatgaccgac atgttgccgc cctgcccgca ggcgatggtg
     4861 tagccgtccg gcgtcgcgtt cgcggccagt cccaggccga tccggccgcc ggcgccgggc
     4921 cggttgtcca cgacgaattg ctggcccagg cgcccggccg cgtacccgcc caggaggcgg
     4981 ccgaaggtgt cgcaggggtc gccgggctgc tggggcacga tgatgcgtac cgcccgggcg
     5041 ggatactttt gcgcgagcgc cgtgccggcc aggcaggcgc aggcgacaag cgcccacagg
     5101 atgcgtgcgg catgagtcat ggttgacctc cctctcaaat cgtagctttt ttgtggaacc
     5161 gccgctaact ctagcgccgc ccgcgtatcg aaagcaagag gcgccccgga ccgaacccgg
     5221 gccagccatt catcgatcgg acagggcaac gttcctgaaa aggcgcctgt cggggcgctg
     5281 ggcggtaacg tctttcaagt gtccgattac cggtgctggg gcagg
//
LOCUS       DBNGSG010000401         7087 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3694367, whole genome shotgun sequence.
ACCESSION   DBNGSG010000401 DBNGSG010000000
VERSION     DBNGSG010000401.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7087)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7087)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7087
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3694367"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..1213
                     /locus_tag="AC158Q_10380"
     CDS             131..1213
                     /locus_tag="AC158Q_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3340222.1"
                     /translation="MMTDDLKLDIFPHIFPPAFFERMKRIAEGNPALGASIMRWLHVP
                     VLWDLDQRIRMMRRFRGYKQILTLSLPAIEYLAGPDESPALARLANDGMAEIVAAHPE
                     QFPGFVASLPMNNVPAALEEMDYAINRLGARGIQIFSNVNGRPLDEPEFFPIFEHCVK
                     RYDLPIWLHPTRTSKFPDYPTETKSKYEIWWLFGWPYETSACMARIVFSGMLQKLPDM
                     KIITHHLGAMAPFFDERIGLGMDQMGLRTHDEDYVGLRKKLGKRPLDYFKMFYGDTSV
                     NGSAPAIRCGLDFFGADRVLFGTDCPFDPEGGPGFIRGNIKAIAKLDLPDAVERKIYY
                     ENAFRMLHMNLALSPGGKSPAKAKKK"
     gene            1426..2166
                     /locus_tag="AC158Q_10385"
     CDS             1426..2166
                     /locus_tag="AC158Q_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR3340223.1"
                     /translation="MRSAGPADRGLIADLVAANRILAMQGVLDGWGHVSVRHNRDPNR
                     YLLSRSLAPELITAKDILEFDLDNNPVNAKGANLYTERFLHGEIYKTRPDVMAVTHFH
                     APPVIPFGIVDVPLQPVFHRAAFIAQGVPVFDIKDAAGLTDLLIRNPSLGQALAKSIA
                     DKPAVLMRGHGATVVGPSLQRLVGRSIFLAMNATLQAQAMALAPGGKIKFLDPEEARL
                     IEAREGYGLARAWEAWKRKAMGSAVERG"
     gene            2196..3461
                     /locus_tag="AC158Q_10390"
     CDS             2196..3461
                     /locus_tag="AC158Q_10390"
                     /inference="COORDINATES: protein motif:HMM:NF048868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Vgb family protein"
                     /protein_id="HIR3340224.1"
                     /translation="MSRSAVVSLMVATMLVAASLAVQAQGKKPSGLPEGAGKEQVERF
                     CIACHQSREILNSSGYTAEGWKELTATMIDLSGVPDDREAIAQYLAQHFPPNNRRAPK
                     LMPGSERVVFKEWQTPTLGARSRDPIQAADGAIWYAGQWVDKIGRINPATGEIKEYAL
                     PARAKPHTVTLDAKGNVWYMGNGNATIGYLDPKTEKITEYKMPDPAAKDPHSAIFDGK
                     GILWFTMQLSNRVGRLDPATGDIRVVEVATPKSRPYGIKISPVDGAPWFACNGRPCIV
                     RIDPATMALTEVKVPGDNATVRRLDFDRDGTIWYVNSGEGRLGRYNPKSGEIKEWPSP
                     SGPKSHPYAIAVVNGIVWYNESAQRPDTLVRFDPRTETFQSWPIPSGRVHAGIIRHMR
                     PTRDGGLVIHQSSTNRIILVTPTRSVAGR"
     gene            complement(3630..3866)
                     /locus_tag="AC158Q_10395"
     CDS             complement(3630..3866)
                     /locus_tag="AC158Q_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340225.1"
                     /translation="MLKVRELYQVEFADGVKGALGIDERGRLYWNGEAVVTDQRVLLP
                     WWVSFAVVACGLSTLVIAMLAVLTYFTLIQAVAV"
     gene            complement(4151..4645)
                     /locus_tag="AC158Q_10400"
     CDS             complement(4151..4645)
                     /locus_tag="AC158Q_10400"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HIR3340226.1"
                     /translation="MNEAKQVTRQQVEDFLYEEAALLDAWRLDDWLKLLTEDAVYNVP
                     SNDRPDADPKDTLFIIADDIRRIRARVTRLKDKSAHAEYPPSRTRRMLSNVRIVDRSG
                     SALKVEANFAVHRFRRDGNVRPYVGHYRYELRVEGGNLKIAKREAVIDAMELGSMGSV
                     SFIL"
     gene            complement(4827..6116)
                     /locus_tag="AC158Q_10405"
     CDS             complement(4827..6116)
                     /locus_tag="AC158Q_10405"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR3340227.1"
                     /translation="MNDETWVINEPEAMRFQVNRSTLVDPQVLALERRRVFDICWIYV
                     GHESEIRAPGDFRTRIVCDRPVIFCRDTRNRVRVFINTCRHRGATVCREAEGNAKSHT
                     CFYHGWSYNRDGELDGVPGQDAFPSSFKKSDFNLKEPPRVASYRGFVFLSFNPKAQPL
                     EDYLAGAKEYIDLVVDQSPSGQMEVIQGVQDYEIRANWKFLTENSFDDYHLLSVHGSW
                     LTYLRNSGVEMKRPERGHLLPGHGVGKVLGNGHGCTDNVNFRGRPVACWIPVYGEAAK
                     PEIERIRAELVARLGDERAARVADTNRNLLIFPNLFINDGSSVTIRTYYPLAPDRMRV
                     LAWALGPVEESPTARAVRLDSFLTFYGPGGFATPDDIEVLESLQANLGTHREVPWMEV
                     TRGMAKEGEQLNTDEPHLRGFWIQWDKLMREKGQDSR"
     gene            6245..>7087
                     /locus_tag="AC158Q_10410"
     CDS             6245..>7087
                     /locus_tag="AC158Q_10410"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3340228.1"
                     /translation="MTSDRGTRCFHLAHAVAAAMAVCAAGSAVAQGAFPARPVQFVVA
                     YTPGTGADILARAFGPRLAESWKVSVVAENRPGATGTIATAFVAKAPADGHTLLFVAT
                     SFGTTPALRDNLTFDPVKDLAPVALIATSGLGVVVHPQLPVKSLKELIVLARRRPGEL
                     HYSSPGNGGPQHLAMELLKLETGINIVHVPYKGFAGAITDVMAGHVQAMVSALQSAHP
                     HVASGRLRMLAVMSGERSPAFPGVPTMKETGLPSLEIYTWYGTFAPAGTPAAAVTRIN
                     ADINA"
BASE COUNT         1358 a         2236 c         2250 g         1243 t
ORIGIN      
        1 agcttctccg atcgcgccgc ccaggaagca ctcggtttct ggcgcgagga gctcggtgag
       61 gcgtgactga tagggcgtga ccgaaaaagc gactggcaac gctgaatcct gcattctgca
      121 tcctgaaatc atgatgactg acgacctcaa gctcgacatt tttccgcaca tcttcccgcc
      181 ggctttcttc gagcgcatga aaaggatcgc ggagggcaat cctgcgctgg gggcgagcat
      241 catgcgctgg ctgcacgttc cggtgctgtg ggatctcgat cagcgcatcc ggatgatgcg
      301 gcgcttccgc ggctacaagc agatcctgac gctgtcgctg cccgccatcg agtacctggc
      361 cggccccgac gagtcgcctg cgctcgcgcg cctcgccaac gacggcatgg cggaaatcgt
      421 cgccgcccac ccggagcaat tcccggggtt cgtcgcctcg ctcccgatga acaacgtgcc
      481 cgccgcgctc gaggaaatgg actacgccat caaccggctc ggggcgcgcg gcatccagat
      541 tttcagcaac gtcaacggcc gcccgctcga cgagcccgag ttcttcccga tcttcgagca
      601 ttgtgtcaag cgctacgacc tgccgatctg gctgcatccc acccgcactt cgaaattccc
      661 cgactacccg accgagacca aatcgaagta cgaaatctgg tggctgttcg gctggccgta
      721 cgagaccagc gcctgcatgg cgcgcatcgt gttctcgggc atgctgcaga agctgccgga
      781 catgaagatc atcacccacc acctgggggc gatggcgccg ttcttcgacg agcgcatcgg
      841 gctcggcatg gaccagatgg gcctgcgcac ccacgacgag gactacgtcg ggctgagaaa
      901 gaagctgggc aagcggccgc tcgactactt caagatgttc tacggtgaca cgtcagtgaa
      961 cggctcggcc ccggcgatcc gctgcggcct ggacttcttc ggcgccgacc gcgtgctgtt
     1021 cggcacggac tgcccgttcg acccggaggg cgggccgggc ttcatccgcg gcaacatcaa
     1081 ggcgatcgcg aagctcgatc tcccggacgc ggtcgagcgg aagatctatt acgagaatgc
     1141 cttcaggatg ctgcacatga acctcgcctt gtcgcccggc ggcaagtcgc ccgcgaaggc
     1201 gaaaaagaaa tagacagaat taacggcaca tgatcaaaaa ttccgcaaac ccgttaagag
     1261 cacaaataga caggattaac aggatttaca ggattagaac gacaggcctc acagttttct
     1321 gaagttattt gctgaatctt gttaatcctg tctattggtg ggtttgacgg gtcttcgaat
     1381 aaataattaa tcctgtctat tgcgatgagc aagagcggag cgaagttgag atcggctggg
     1441 ccggcggacc gcgggctgat cgcggatctg gtggcggcga accgcatcct ggcaatgcag
     1501 ggggtgctgg acgggtgggg ccacgtcagc gtgcggcaca accgggaccc caaccgctac
     1561 ctgctgtcgc gctcgctcgc tcccgagctg atcaccgcga aggacatcct cgagttcgac
     1621 ctcgacaaca acccggtgaa cgcgaagggt gcgaacctct acaccgagcg cttcctccac
     1681 ggcgagatct acaagacgcg gcccgacgtc atggcggtca cgcatttcca cgcgccgccg
     1741 gtgattcctt tcggcattgt cgacgtgccg ctgcagccgg tttttcaccg cgccgccttc
     1801 atcgcccagg gcgtgccggt cttcgacatc aaggacgccg ccggcctgac ggacctgctg
     1861 atacgcaatc cgtcgctcgg gcaggcgctg gcgaagagca tcgccgacaa gcccgcggtg
     1921 ctgatgcgcg gccacggcgc gaccgtggtg gggccgtcgc tccagcgcct ggtcggccgc
     1981 agcatcttcc ttgccatgaa cgccacgctg caggcgcagg ccatggcgct tgcaccgggc
     2041 gggaagatca agttcctcga cccggaggag gcccgcctga tcgaggcacg cgaaggctac
     2101 gggctggcgc gggcgtggga ggcatggaag cgaaaagcaa tgggaagcgc ggttgaacgc
     2161 gggtagactg ggttttttta tttgagggag gtgatatgtc cagatcggca gtcgtgtcct
     2221 tgatggttgc cacgatgctc gtcgcggcgt cgcttgccgt ccaggcacag ggaaagaagc
     2281 cgtccggcct gccggaaggc gccggcaagg agcaggtgga aaggttctgc attgcctgcc
     2341 accagtcgcg ggaaatcctc aacagctccg gctacaccgc cgagggctgg aaagagctca
     2401 ccgccacgat gatcgacctg tccggcgttc cggatgacag ggaggcgatc gcgcagtacc
     2461 tcgcgcagca ttttccgccg aacaaccgcc gcgcgcccaa gctgatgcct ggaagcgagc
     2521 gcgtcgtgtt caaggaatgg cagaccccga cgctcggcgc gcgttcgcgc gaccccatcc
     2581 aggccgccga cggcgcaatc tggtacgccg gccagtgggt cgacaagatc gggcggatca
     2641 atcccgcgac cggcgagatc aaggaatacg cgctgccggc ccgggcgaaa ccccacaccg
     2701 tcaccctcga tgcgaagggc aacgtatggt acatgggcaa cggcaacgct accatcggct
     2761 acctcgatcc gaagacggag aaaatcaccg agtacaagat gccggacccg gcggcgaagg
     2821 acccgcattc ggcgattttc gacgggaagg gaatactctg gttcaccatg cagttgagca
     2881 atcgggtcgg caggctggat cccgcgaccg gcgacatccg ggtcgtcgag gtggcgacgc
     2941 cgaaatcgcg gccgtacggc atcaagatct ccccggtcga cggcgcgccg tggttcgcgt
     3001 gcaacggccg cccctgcatc gtgcgcatcg acccggcgac gatggcgctc acggaagtca
     3061 aggtgccggg tgataacgcg acggtgcggc gcctcgactt cgaccgcgac ggcacgatct
     3121 ggtacgtcaa ctcgggcgag ggccggctcg gccgctacaa cccgaagtcg ggcgagatca
     3181 aggagtggcc gtcgcccagc gggccgaagt cgcatcccta tgccatcgcg gtggtgaacg
     3241 gcatcgtctg gtacaacgag tcggcccagc ggccggacac gctggtgcgc ttcgatcccc
     3301 gaaccgagac gttccagagc tggcccattc cgtccggccg cgtccacgcc ggcatcatcc
     3361 ggcacatgcg cccgacccgg gacggcggcc tggtgatcca ccagagcagc accaaccgca
     3421 tcatcctggt gacgcccacg cgcagcgtcg cgggccggtg aagttcctca tgtaaccgct
     3481 gatgaacgcc gatacacgcc gataacttca agaccgctaa gccacaggga gcacggagag
     3541 cgcagagaaa gaagcggtgg atacagcaaa acggccggct gagaattgcc ggccgttttg
     3601 atttttgtta gtcgagatag gagacagcat tacacggcaa cagcctggat caaggtgaaa
     3661 taagtcagga ccgccagcat ggcgatgacg agcgtggaca agccgcacgc gacgaccgca
     3721 aaactcaccc accagggaag aagcaccctt tgatcggtga cgacagcctc gccgttccag
     3781 tacaggcgcc cgcgctcgtc gatgcccaga gcgcccttga cgccgtccgc aaactcgacc
     3841 tggtacaact cgcgaacttt gagcacgacg gtcctcccga ttggtgactt gccgtaggca
     3901 ttgacaggtc ggccgcgggt tagtttctgg ggaagcagtg acgaagtgaa gcagtgacga
     3961 ggtggcgata gcccggggcc gccgatgaac gccgatacac gccaataact tcaagaactt
     4021 cagccacaaa gagcgcaggg gatacggagg gacgttaaag gggccagatt cgattgaagg
     4081 ttgaagttct tggcggcctt ggcgtcttgg cggttcaagt ctttcgccac tgtgtcactt
     4141 cgtcaccact tcatagtatg aagctgaccg accccatcga tcccagctcc atcgcatcga
     4201 tgacggcctc gcgcttcgca atcttcaggt tcccgccctc gacccgcagc tcgtagcggt
     4261 agtgcccgac atacgggcgc acgttcccgt cgcggcggaa gcgatgcacg gcgaaattgg
     4321 cttcgacctt cagcgccgag ccgctgcgat ccacgatgcg cacgttggag agcatgcggc
     4381 gcgtgcgcga gggcgggtac tcggcgtggg cgctcttgtc cttcagccgc gtgacgcgcg
     4441 cgcgaatgcg gcggatgtcg tcggcgatga taaacagcgt gtccttcggg tccgcatccg
     4501 ggcgatcgtt cgacgggacg ttgtagacgg catcctccgt caggagcttc agccagtcgt
     4561 cgagccgcca agcgtcgagg agggcggcct cctcgtagag gaagtcctcg acctgctggc
     4621 gcgtgacctg cttcgcttcg ttcatgccaa gaatagacag gattgaaatt caaataattc
     4681 gtttaaaccc gccaacaccg aaaaaataga caggattaac aggattaaca agattaaaca
     4741 attcgaaaac taaaacgctg attggtcttt tttgatctaa tcctgtaaat cttgttaatc
     4801 ctgtctatga gtgattttga cgggtgttaa cgcgagtcct gtcccttctc acgcatcagc
     4861 ttgtcccact ggatccagaa cccccgcagg tgcggctcgt cggtgttcaa ctgctcgcct
     4921 tccttcgcca tcccccgcgt cacctccatc cacggcactt cgcgatgcgt gccgagattc
     4981 gcctgcagcg actccagcac ttcgatgtcg tccggggtgg cgaagccgcc cgggccgtag
     5041 aaggtgagga agctgtcgag gcgcacggcg cgggcggtgg gggattcctc caccggcccc
     5101 agcgcccagg cgagcacgcg catgcgatcg ggcgccagcg ggtaatacgt gcggatggtg
     5161 accgacgagc cgtcgttgat gaacaggttg gggaagatga gcaggttgcg gttggtgtcg
     5221 gcgacgcgcg cggcgcgctc atcaccgagg cgggcgacca gttcggcgcg aatgcgctcg
     5281 atctcgggct tcgccgcctc gccgtaaacg gggatccagc aggccaccgg ccgcccgcgg
     5341 aagttcacgt tgtcggtaca accgtggccg ttgccgagca ccttgcccac gccgtggccg
     5401 ggcagcaggt ggccgcgctc gggacgcttc atctcgacgc cggaatttcg caggtacgtc
     5461 agccaggagc cgtggacgga gagcaggtgg tagtcgtcga agctgttctc ggtcaggaat
     5521 ttccagttgg cgcggatttc gtagtcctgc acgccctgga tcacttccat ctgtccggat
     5581 ggagactggt cgacgacgag gtcgatgtat tccttcgcgc ccgcgaggta gtcctccaat
     5641 ggctgcgcct tcgggttgaa gctgaggaag acgaagccgc ggtagctcgc cacgcgcggc
     5701 ggctccttca gattgaagtc gctcttcttg aagctcgacg ggaacgcgtc ctggccgggc
     5761 acgccgtcca gctccccgtc gcggttgtag ctccagccgt gatagaagca ggtatggctc
     5821 ttcgcgttgc cttcagcctc gcggcagacg gtcgcgccgc ggtggcggca ggtattgatg
     5881 aagacgcgaa cgcggttccg ggtgtcgcgg cagaagatga cgggccggtc gcagacgatt
     5941 cgggtcctga agtcgccggg cgcgcggatt tccgactcgt ggccgacgta gatccagcag
     6001 atgtcgaata ccctgcgccg ctcgagcgcg agcacctgcg ggtccaccag ggtggagcgg
     6061 ttgacctgga agcgcatcgc ttccggctca ttgatcaccc aagtctcgtc gttcacgcgc
     6121 gtgccatctt caaatcgggc aagtgttgtc gtaagactgc tcacttgtca atcgcggcct
     6181 gcgggaatcg cccgttttct gcataatagg aaccttccaa aaacaatcca tccgcggagg
     6241 ggccatgacc agcgatcgag gaacccggtg ttttcacctt gcccacgccg tggccgccgc
     6301 catggccgtc tgcgcagccg gcagcgccgt cgcgcagggt gccttcccgg cacgcccggt
     6361 gcagttcgtg gtggcgtaca cgcctggcac cggcgccgac attctcgcgc gcgccttcgg
     6421 cccccggctc gccgagagct ggaaagtcag cgtcgttgcc gaaaaccggc ccggggcgac
     6481 cggcaccatt gcgacggcgt tcgtcgcaaa ggcgccggcg gatggccaca ccctgctgtt
     6541 cgtggcgaca tccttcggca ccacgcccgc gctgcgcgac aacctgacgt tcgacccggt
     6601 gaaggacctc gctccagtgg cgttgatcgc gaccagcggc cttggcgtgg tcgtgcatcc
     6661 gcaactgccg gtgaagtcgc tgaaggaact gattgtcctc gccaggcgcc gtcccggcga
     6721 gctccattac tcctcgcccg gtaacggcgg cccccagcac ctcgccatgg aactgctcaa
     6781 gctcgaaacc gggatcaata tcgtgcatgt gccgtacaag ggtttcgccg gcgcgatcac
     6841 cgatgtcatg gcgggacacg tgcaggccat ggtttccgcg ctgcagagcg cgcatccgca
     6901 cgtcgcgagc gggcgactgc gcatgctggc ggtgatgagc ggggagcgct cacccgcgtt
     6961 ccccggcgtg ccgacgatga aggagacggg ccttcccagc ctggagatat acacgtggta
     7021 cggcacgttc gcgccggccg gcacgcccgc ggcggcggtg acccgcatca acgccgatat
     7081 caatgcg
//
LOCUS       DBNGSG010000402         5319 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2845967, whole genome shotgun sequence.
ACCESSION   DBNGSG010000402 DBNGSG010000000
VERSION     DBNGSG010000402.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5319)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5319)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5319
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2845967"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="AC158Q_10415"
     CDS             complement(<1..204)
                     /locus_tag="AC158Q_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HIR3340229.1"
                     /translation="MTQHHGALQSALPQNPPVPRGREWRVVPMLLPYLWEYRWRVSIA
                     LVFLVAAKLANVAVPLVLKEVVDG"
     gene            347..700
                     /locus_tag="AC158Q_10420"
     CDS             347..700
                     /locus_tag="AC158Q_10420"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HIR3340230.1"
                     /translation="MDVIFISDLRLDILIGVYDWEKQVPQKVQLDLEIGLPGRKAGGD
                     KLGDTIDYAAVVSRIEGTLAENRFGLLETLADHLAGIAMKEFRSPWVKVSVTKLAPLK
                     NVKRLGVTIERGTKA"
     gene            complement(779..1672)
                     /gene="xerD"
                     /locus_tag="AC158Q_10425"
     CDS             complement(779..1672)
                     /gene="xerD"
                     /locus_tag="AC158Q_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HIR3340231.1"
                     /translation="MTGKTSLIDEFCDALWLEDGLAKNTLDSYRGDLAQYAAWLEKNG
                     KSLLAASNADIQAYLGVFHKRKTRASSAARLLSSLKRFYRYSLRQGKLKSDPTLKIDS
                     PKLPRSLPRSLTEEDVEGLLAAPPVGKALGLRDKAMLETIYASGLRVSELVALKLGQV
                     SQDMGVVRVIGKGSKERLVPLGEEALAWIRRYLKEARPELLGGRSSDDLFITARGSAM
                     SRQMFWHLLRRYALRAGLRKSISPHTLRHAFATHLLNHGADLRVVQLLLGHSDISTTQ
                     IYTHVARERLKQLHAKHHPRG"
     gene            complement(1676..2131)
                     /locus_tag="AC158Q_10430"
     CDS             complement(1676..2131)
                     /locus_tag="AC158Q_10430"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008325765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HIR3340232.1"
                     /translation="MATPFAVLGIHTAGESLTRIEYLPKGAATLDPVDRFAARVCRQI
                     ECYLDDADFRFDLPFEYRGTAFQCRVWRKIHAIPSGSTLTYLDVARCLGSAPRPVGGA
                     CGANRLPLVIPCHRVLGSGGIGGFMHMRRGPGIEIKRWLLRHEGVTCDR"
     gene            complement(2148..4391)
                     /locus_tag="AC158Q_10435"
     CDS             complement(2148..4391)
                     /locus_tag="AC158Q_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HIR3340233.1"
                     /translation="MSEKDDRLIHEIAAEHHIAVDEAALKKAEEYIEAEEGATNRLSG
                     LLGHLVVVVAVVMSLFHLYAAYAIVPTQELRMIHVGFVLFLVFLLFPFSRRYRHRVMW
                     WDGIAVFLSLLVIGYGLLGGDDFTDRSTIPTNLDMVFGVILTALVLEAARRGTGWIMP
                     FVCVLFVAYAMLGPHLGEPWTHRGYDISRLVGHMYMTLEGIFGVPVDVSSSLIILFTI
                     YGAFLQFSGAGKFFIDVSVSAMGGKPTGAGRTIVLSSFLLGGPSGSGVATTVTLGTVA
                     YPMLAKAGYGKDAAGGLLAAGGLGAIISPPVLGAAAFLIAEFLKISYLDVILMAVIPT
                     CLYYFALFLMVEIDAHKFGMKNIPFDKTDTLWNLTKQFGFHFLSLVAIIFFMLLGFSP
                     VSAVFWATMVAFVMSAMHPDCALMSRRQMILFCIVAVAVCVIYSAVKGRFHQDALTWA
                     LPAGALALIAAAFAGRAWRLWPAKLVKALEVGSVGVLNVAATCAAAGIIVGVVTLTGL
                     GLKFSSIVISYADGLTQGTTGLLSAVGVEITKALVHDVKLLYTAIFTSLIVWIVGLAV
                     PVTASYIICAVIAAPALITLGVPDFAAHMFIFYYAVLSEVSPPTALSPFAAAAITGGD
                     PYKTTLQSWKYTMPAFLVPFMFVLDPAGLGLLLMGSFKGLASASVVDIAAVSVTAMFG
                     IAALACGMQGWVFRKTTSLERWMLILAGLALVYPRAAFDYIGLALLIAVVVMQKLRRV
                     ETPAAPA"
     gene            complement(4489..>5319)
                     /locus_tag="AC158Q_10440"
     CDS             complement(4489..>5319)
                     /locus_tag="AC158Q_10440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HIR3340234.1"
                     /translation="MANILSKYVPGLNATAESTAGSIANLEFILTKKADVALSMADAS
                     YDAWKGEQRFKGRPVPVRGLMVLYPNRFHIVTLEGLGINKVADLKGRRVSTGPPRSGT
                     EVKANRVMEAAGLNPEKDITRERLTVQESGNALKDRKIDAFFWSGGIPTAAVTDLAAS
                     PGVKIKFIEHDELVAPLNKKFGPLYVKDIIPAKSYPGQEKDAKVSTIWNILVTGADTP
                     DEVAYNIVKTMFERKEDMVRVHKESENFDLKYQTNAAIVIPFHPGAAKYFREKGLKVK
                     "
BASE COUNT         1006 a         1746 c         1696 g          871 t
ORIGIN      
        1 cccgtcgacc acctccttta gcaccagcgg cacggcgacg ttggcgagct tggcggcgac
       61 caggaacacg agcgcgatcg acacgcgcca gcggtactcc cacaggtacg gcagcagcat
      121 cggcaccacg cgccattcgc gcccacgcgg caccggcggg ttttgcggca aggcactttg
      181 tagggcgccg tgatgctgcg tcatatcagg ggtgaggggg atgagtcaaa agtgtcgagg
      241 agggtgtgtc gagggattgg agaagaatgg ccaggaggtc gctcgccccc cccactcgac
      301 acccgacact cgacacccga cactcgacac tccgcgatag cgccgaatgg atgtcatctt
      361 catcagcgac ctgcgcctcg acatcctgat cggagtctac gactgggaaa agcaggtgcc
      421 gcagaaggta cagctcgatc tcgagatcgg cctgcccggc cgcaaggcgg gcggggacaa
      481 gctgggtgac accatcgact acgcggcggt cgtatcacgc atcgagggca ctctcgccga
      541 aaaccgcttc gggctgctgg aaaccctcgc cgatcacctc gccggaatcg ccatgaagga
      601 gttcaggtcg ccctgggtca aggtcagcgt caccaagctc gcgccgctga aaaacgtgaa
      661 gcggctcggc gtcaccatcg aacgaggcac gaaggcgtga taagtgacga gtgataggtg
      721 ataagagacc cgcgcgtcgc tcggaactcg acccctatca cttatcacct atcacttttc
      781 accctcttgg atgatgcttc gcatgcagct gcttcagccg ttcccgcgcc acgtgggtat
      841 agatctgcgt cgtggagatg tcggaatggc cgagcagcaa ttgcactacg cgcaggtccg
      901 cgccgtgatt cagcaggtgc gtggcgaagg cgtggcgcag cgtgtggggt gatatcgact
      961 tccgcagccc ggctctcagg gcgtagcggc gcagcaggtg ccagaacatc tggcgcgaca
     1021 tcgcgctgcc gcgcgcggtg atgaacaaat cgtcgctcga gcgcccgccg agcagttcgg
     1081 ggcgcgcctc tttcagatag cgccgtatcc aggcgagcgc ctcttcgccc agcggcacca
     1141 gccgctcctt cgagcccttg ccgataacgc gcaccacgcc catgtcctgg ctcacctggc
     1201 cgagcttcag cgcaaccagt tcggatacgc gcaggccgct cgcataaatc gtctcgagca
     1261 tcgccttgtc gcgcagcccc agcgccttcc ccaccggcgg cgcggcgagc aggccttcga
     1321 cgtcctcctc ggtcaggctc ctgggcagcg accgcggcag tttgggagag tcgattttca
     1381 gcgtcggatc ggatttgagt ttcccctgcc gcaggctgta gcggtaaaag cgcttcaggc
     1441 tcgaaagcag ccgggctgcg ctcgatgccc gcgtcttgcg cttgtgaaaa accccgaggt
     1501 acgcctggat gtccgcgttg ctcgcggcaa gcaggctctt gccgttcttt tccagccacg
     1561 cggcatattg cgcaaggtcc ccgcgataac tgtcgagcgt gttcttcgcg agcccgtcct
     1621 cgagccacaa ggcatcacag aattcgtcga tgaggctggt cttgcccgtc acgggtcacc
     1681 ggtcacaggt cacgccctca tgcctcagca gccagcgctt gatctcgatg ccgggaccgc
     1741 gccgcatgtg catgaacccg ccgatcccgc cactgccgag aacgcggtgg cagggtatca
     1801 ccagcgggag gcggttggcc ccgcaggcgc cgcccacggg ccggggggcg ctgccgaggc
     1861 agcgggcgac atcgagatag gtgagcgtac tgccgctggg gatcgcgtgt atcttgcgcc
     1921 acacacggca ctggaacgcg gtgccgcggt attcgaacgg caggtcgaat cggaagtccg
     1981 catcgtcgag atagcactcg atctgccggc acacgcgcgc ggcaaagcgg tcgaccggat
     2041 cgagcgtcgc ggcgcccttg ggcaagtact cgatgcgggt cagggactcg cccgccgtgt
     2101 ggatgccgag cacggcgaac ggcgtcgcca tgcgcgcagt acaaatatca agcgggggca
     2161 gcgggggttt cgacacggcg cagtttttgc atcactacca ctgcgatcag aagcgcaagc
     2221 ccaatataat cgaacgccgc gcgcgggtaa accagcgcga gaccggcgag gatgagcatc
     2281 cagcgctcga ggcttgtggt cttcctgaag acccagccct gcatgccgca ggcgagcgcg
     2341 gcgatgccga acatcgcggt cacggacacg gccgcaatgt ccaccaccga agcgctcgcc
     2401 agacccttga acgagcccat caacagcagc cccaggcccg ccgggtcgag cacgaacatg
     2461 aacggcacca ggaacgcggg catcgtgtac ttccacgatt gcagcgtcgt cttgtagggg
     2521 tcgccgccgg tgatcgcggc cgcggcgaac ggcgagagcg cggtgggagg agacacttcg
     2581 gacagaaccg cgtaatagaa gatgaacatg tgcgccgcga aatccggcac cccgagcgtg
     2641 atcagcgccg gggcggcgat cacggcgcag atgatgtagg acgccgtcac cggcacggcg
     2701 agcccgacga tccagacgat gagcgatgtg aagatggcgg tgtagagcag cttcacgtcg
     2761 tgcaccagcg ctttggtgat ctcgaccccg accgccgaca gcaggcccgt cgtgccctgc
     2821 gtgagcccgt cggcgtaact gatcacgatc gagctgaact tgagcccgag ccccgtcagc
     2881 gtcaccacgc ccacgatgat gccggccgcc gcgcacgtag cggcgacgtt caacacgccg
     2941 accgagccca cctccagcgc cttgaccagc tttgccggcc agaggcgcca cgcacgcccg
     3001 gcaaatgcgg ccgcgatgag cgccaacgca ccggccggta gcgcccaggt gagcgcgtcc
     3061 tggtgaaaac ggcccttgac cgccgaatag atgacacata ccgcgaccgc gacgatgcag
     3121 aagaggatca tctggcgccg gctcatcagc gcgcagtcgg gatgcatcgc gctcatgacg
     3181 aacgccacca tggtcgccca gaacaccgcc gacaccggcg agaagccgag cagcatgaaa
     3241 aaaatgatcg ccaccagcga caggaagtga aaaccgaatt gcttcgtcag gttccagagg
     3301 gtatcggtct tgtcgaacgg aatgttcttc atcccgaact tgtgcgcgtc gatctcgacc
     3361 atgaggaaca gcgcgaaata gtagaggcag gtcggaatga ccgccatcag gatcacgtcg
     3421 aggtacgaga tcttgaggaa ctccgcgatc aggaacgcgg ccgcgcccag caccggcggc
     3481 gagatgattg cgcccaggcc gccggcggcg agcagtccgc ctgccgcgtc cttgccgtag
     3541 cccgccttcg ccagcatcgg gtacgccacg gttcccagcg tcacggtggt cgcgacgccg
     3601 ctgcccgagg gcccgcccag cagaaacgag gagagcacga tggtgcgccc cgcgcccgtc
     3661 ggcttgccgc ccatcgcgga gaccgagaca tcgatgaaga acttgccggc gccggagaac
     3721 tgcaggaaag cgccgtaaat cgtgaagaga atgatgagcg aggaggagac gtccaccggc
     3781 acgccgaaaa ttccctcgag cgtcatgtac atgtggccga ccagccgcga gatgtcgtag
     3841 ccgcggtgcg tccacggctc gccgagatgc gggccgagca tcgcgtacgc cacgaacagc
     3901 acgcacacga acggcatgat ccagccggtg ccgcggcgcg ccgcctccag cacgagagcc
     3961 gtcaggatca cgccgaacac catgtccagg ttcgtgggga tggtgctgcg gtcggtgaag
     4021 tcgtcgccgc cgaggaggcc gtagccgatg acgagaaggg atagaaatac cgcgatcccg
     4081 tcccaccaca tgacgcggtg gcgatatcgg cgcgagaacg ggaacagcag gaacaccagg
     4141 aacaggacga agccgacgtg gatcatgcgc agctcctgcg tcggcacgat cgcataggcg
     4201 gcgtagaggt ggaagagcga catcaccacg gccaccacca ccacgaggtg gccgagcagg
     4261 ccggagaggc ggttggtcgc gccctcttcc gcctcgatgt actcctcggc tttcttgagc
     4321 gccgcctcgt cgacggcgat gtggtgctcc gccgcgatct cgtgtatcag ccggtcgtcc
     4381 ttctcgctca tcgtgcgccc ctcccgtcgc cggtcccgcg cttcccccaa aaaaaatcca
     4441 gcgcgtgggt cgcgctggat ttccgtcagg tccgcgacga tccgcgggct acttgacctt
     4501 cagccctttt tcgcggaaat acttggccgc ccccgggtgg aacgggatca cgatcgccgc
     4561 gttggtctgg tacttgagat cgaagttctc cgactccttg tgcacccgga ccatgtcttc
     4621 cttgcgctcg aacatcgtct tcacgatgtt gtaggcgacc tcgtccgggg tgtccgcgcc
     4681 cgtcaccagg atgttccaga tggtgctgac tttcgcgtcc ttttcctggc ccggatagga
     4741 tttcgccggg atgatgtcct tcacgtagag cgggccgaat ttcttgttca gcggtgcgac
     4801 cagctcgtcg tgctcgatga acttgatctt gacgcccggc gaggccgcca ggtcggtcac
     4861 cgcggcggtc ggaatgccgc ccgaccagaa gaaggcgtcg atcttgcggt ccttcaacgc
     4921 gttacccgac tcctgcaccg tgaggcgctc gcgcgtgatg tccttctcgg ggttcagccc
     4981 cgccgcttcc atgacgcggt tcgccttgac ttcggtgccg ctgcgcggtg ggccggtgga
     5041 cacgcgccgc cccttgagat cggcgacctt gttgatgccc agcccctcga gcgtgacgat
     5101 gtgaaagcgg ttcgggtaga gcaccatcag tccccgcacc ggcaccggcc gccccttgaa
     5161 tctctgctct cccttccagg catcgtagct ggcgtccgcc atcgagagcg cgacatccgc
     5221 cttcttggtc aggatgaact cgaggttggc gatcgagccc gcggtggatt ccgccgtcgc
     5281 gttaagcccc ggcacgtact tggacaggat gttcgccat
//
LOCUS       DBNGSG010000403         2926 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4543876, whole genome shotgun sequence.
ACCESSION   DBNGSG010000403 DBNGSG010000000
VERSION     DBNGSG010000403.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4543876"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="AC158Q_10445"
     CDS             <1..387
                     /locus_tag="AC158Q_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340235.1"
                     /translation="VIGYFTRGRHPTHLVMRRLRRMSGFYAPRLSTTHFPPTFNPIEE
                     YRMKISSIVFATLFALMSGAAFASSCPKHMKAIDAALPKAKLSAAQMSEVKKLRADGE
                     AFHKAGKHGDSMEALGKAEAILGIKM"
     gene            complement(495..1958)
                     /locus_tag="AC158Q_10450"
     CDS             complement(495..1958)
                     /locus_tag="AC158Q_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019719842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HIR3340236.1"
                     /translation="MKRKMIVGALAAAGLAAALFAGGYALYQPLMSHARAEAVAAAEP
                     AAPPVVRGLPDFSLLVERYGPAVVNVSVTQTVRTAAGGAQPRGLDPNDPFFEFFRRFQ
                     APVPRDGVPAQGQGSGFIVSPDGLVLTNAHVVRNASEVTVRLTDKREFKAKVVGSDPQ
                     TDVAVLKIEAANLPVVKFGKSDDVRVGEWVVAIGSPFGFENSVSAGIVSAKGRALPDG
                     TYVPFIQTDVAVNPGNSGGPLFNLKGEVIGINSQIYSRTGGYQGLSFAIPMELAARIR
                     DQIVQFGKVTRGRIGVTIQEVSQPLADSFGLKRPGGALVSSVEKNGPAEKAGLETGDV
                     ILRIDGRDIGRSIDLSSRVAELRPGTRARLEVWRKGAPREIDVTVGEFKSAQAASGPA
                     AGAEEPGRLGVAARTLTPEEQQALGVSGGVLVEHVDPNGAAAKSGIRQGDVILALNST
                     PVSSIEQLGNVVAKSGKRAALLVRREDARIFVPVELG"
     gene            complement(2178..2885)
                     /locus_tag="AC158Q_10455"
     CDS             complement(2178..2885)
                     /locus_tag="AC158Q_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3340237.1"
                     /translation="MDKRIAVVTGGNRGIGFEICRQLAKLGGIRVVLTARDEAKGQAA
                     AKKLRGEGLEVDIHALDVTSEMSVKTLAADLEKKYGRCDVLVNNAGVMLDPRGSRVLD
                     SSLGTWRATLDTNLIGPLLLIQTLAPIMKKNRYGRIVNVSSGQGQLSDMGAGTPAYRV
                     SKTALNALTRTFAAELNGSGVLVNAMCPGWVKTDMGGSSAPRRPEQGADTALWLATLP
                     DDGPSGGFFRDRKPIPW"
BASE COUNT          471 a         1031 c          917 g          507 t
ORIGIN      
        1 gttattgggt attttactcg cgggcggcat ccgacgcatc ttgtcatgcg ccgcttgcgc
       61 aggatgtccg ggttttacgc accacgcttg agtactacgc actttccacc gaccttcaac
      121 ccgatagagg agtaccgtat gaaaatcagc agcattgtgt tcgcaaccct gttcgcactg
      181 atgagcggcg ccgccttcgc cagcagctgc cccaagcata tgaaggccat tgacgcagcg
      241 ttgccgaaag ccaagctctc cgccgcgcag atgtccgaag tgaagaaact gcgcgccgac
      301 ggagaagctt tccacaaagc aggcaagcac ggcgattcga tggaagccct gggcaaggcg
      361 gaggcgatcc tcggcatcaa gatgtaagcg gcctttttgg cgttacgtcc cctcgcctgc
      421 gcggcggggg cgcacaaaaa aatccccgcc acagtggcgg ggattttttg tgctttccgc
      481 gaatacgcgt cgcgtcagcc cagttccacg ggcacgaaga ttctcgcatc ctcgcgccgc
      541 accagcagcg cggcacgctt gccggatttc gcgacgacat tgccgagctg ctcgatactc
      601 gataccggcg tgctgttcag cgcgagaatg acgtctccct gccggatgcc cgacttcgcc
      661 gccgctccgt tcgggtccac gtgctcgacc agcacgccgc cgctcacgcc caacgcctgt
      721 tgctcctccg gcgtaagcgt gcgcgcggcg actcccaggc gccccggctc ctctgctccg
      781 gccgccgggc cggacgcggc ctgggcgctc ttgaattcgc ccacggtcac gtcgatctcg
      841 cgcggcgcgc ccttgcgcca tacctccagc ctggcgcggg tgccgggccg caactcggcg
      901 acccggctcg aaaggtcgat cgagcggccg atgtcccggc cgtcgatcct gaggatcacg
      961 tcgccggttt ccaggcccgc cttctccgcg gggccgttct tctccaccga gctcaccagc
     1021 gcgccgccgg gccgcttgag gccgaacgaa tcagcgagcg gctggctcac ctcctggatg
     1081 gtgaccccga tgcgtccgcg cgtaaccttg ccgaactgga cgatctggtc gcggatcctg
     1141 gctgctagtt ccatcgggat cgcaaatgac aagccctggt agcccccggt gcggctgtag
     1201 atctgcgagt tgatgccgat cacctcgcct ttcaggttga acagcggccc gccggagttg
     1261 ccgggattga ccgcgacatc ggtctggatg aagggcacgt aggtgccgtc gggcagcgcg
     1321 cgccccttgg cgctgacgat gcccgcgctc accgagttct cgaaaccgaa cggcgagccg
     1381 atcgcaacca cccattcgcc cacgcgcacg tcatccgact ttccgaactt caccaccggc
     1441 aggttcgcgg cctcgatttt cagcaccgcg acgtcggtct gcgggtcgga acccacgacc
     1501 ttcgccttga actcgcgttt gtcggtcagc ctgacggtaa cctcggacgc gttccgcacc
     1561 acgtgggcgt tggtgaggac cagcccgtcc gggctcacga tgaagcccga gccctgcccc
     1621 tgggccggga ccccgtcccg aggcaccggg gcctggaagc gccggaagaa ctcgaagaac
     1681 gggtcgttgg gatccaggcc gcggggctgt gctccgcctg ccgcggtcct gaccgtctgc
     1741 gtcacgctga cgttgacgac agcaggcccg tagcgctcca ccaggagcga aaagtcaggc
     1801 agcccccgca ccaccggcgg tgcggccggc tcggctgccg cgaccgcttc cgcccgggcg
     1861 tgcgacatca gcggctggta gagcgcgtat ccgccggcga agagggccgc ggccagcccg
     1921 gccgcggcca atgcacccac tatcattttc cgtttcatga ctctttctcc gtataagaat
     1981 gcgcggccgg gtggcggcac cggaccgcgc ggtcgcgcgc gagcagcgcc gcgggacggg
     2041 cctccggccg gggaccaacg ctatgactac gcggcatttg accgcggcgc ccgcgccagg
     2101 tttcgtccag gaatacggcc cgcggggatc cgggtatcgg gaaccttatc ggggcggcca
     2161 atgcggcgcc gccccgatca ccagggtatc ggcttgcggt cgcggaagaa tccgccgctg
     2221 ggcccgtcgt ccggcagcgt cgccagccat agcgcggtat ccgccccctg ttccggcctg
     2281 cgcggcgccg aagatccgcc catgtcggtc tttacccagc ccgggcacat cgcgttgacg
     2341 agcaccccgc tgccgttcaa ctccgccgcg aacgtgcggg tcagtgcgtt caaggcggtc
     2401 tttgagacac ggtaggccgg cgtgccggcg cccatgtccg agagttgccc ctgcccgctc
     2461 gagacgttga cgatgcgccc gtaacggttc ttcttcatga tcggcgcaag cgtctggatc
     2521 aagagcagcg ggccaatcag gttggtgtcc agcgtcgcgc gccaggttcc caggctggag
     2581 tcgagcacgc gtgagccgcg cgggtcgagc atcacgccgg cgttgttgac gagcacgtcg
     2641 caccgcccgt atttcttctc caaatccgcc gccagcgttt tgacgctcat ctcactcgtg
     2701 acgtcgagcg catgaatatc gacttccaga ccctcgccgc gcagcttctt cgccgccgcc
     2761 tgccccttcg cctcgtcgcg cgcggtcagg acaacgcgaa tgccgccgag cttcgcaagc
     2821 tgccggcaga tctcgaaacc gatgccgcgg tttccgcccg tgacgacggc tattcgcttg
     2881 tccatgtcgg gctacagctt ggacacagag gacacagagg acacag
//
LOCUS       DBNGSG010000404         9361 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3695629, whole genome shotgun sequence.
ACCESSION   DBNGSG010000404 DBNGSG010000000
VERSION     DBNGSG010000404.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9361)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9361)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9361
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3695629"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..932
                     /gene="hpnD"
                     /locus_tag="AC158Q_10460"
     CDS             99..932
                     /gene="hpnD"
                     /locus_tag="AC158Q_10460"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046905 - 15-cis-phytoene synthase
                     activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HIR3340238.1"
                     /translation="MDPHDYCQTKAAASGSSFYYSFLFLPPERRRAITALYAFCREVD
                     DVVDECSDAAVARMKLAWWRKEVAAAYHGTPQHPVARALSEVAPRFNLSEERLNEIID
                     GMQMDLDYNRYPDFDTLKLYCHRVASVVGLLSAEIFGYQDRRTLDYAADLGMAFQLTN
                     IIRDVGEDARRNRVYLPLDEIARHGMSESDILQARETDAFRRLMEFQIRRALGYYRDA
                     FAKLPAGDRRAQRPGLVMAAIYQTTLDEIRRDGCRVLTQRVSLTPIRKLWIAWRTWVK
                     G"
     gene            1074..1829
                     /locus_tag="AC158Q_10465"
     CDS             1074..1829
                     /locus_tag="AC158Q_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3340239.1"
                     /translation="MDLQLKGKTALVTGASMGIGRAIAKGLAAEGARICIAARRGNLL
                     EELAKEIAAAGGARPGIVEIDVLPEGAPARLARAALDALGHIDILVNSAGGSKPAIPI
                     DAPEEAWEESMTLNFVRVRQLTHAMLPGMIGKKWGRVINISGKSEPERLIAATPTKAA
                     IHAWSKGLSREVGRYGITVNSIAPGRIMSEQIRRKYSEEFRAKQSAEEIPVGRYGEPE
                     ELAALAVFLASPVAGYITGAVLPVDGGLRRYAF"
     gene            2005..3123
                     /locus_tag="AC158Q_10470"
     CDS             2005..3123
                     /locus_tag="AC158Q_10470"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006384417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HIR3340240.1"
                     /translation="MQTKIPCVIMRGGTSRGPFFKLTDMPADIPTRDAVLLSVMGSPH
                     QIQIDGIGGSHSVTSKVAMISKSKRPDADVDYLFAQVQIHESIVDTKPNCGNMLVAVG
                     PFAIDAGLVPAKNGETTVRIFNVNTQSLVEAIVQTPGGQVRYDGDAAIDGVPGTAAPV
                     GINFQSAIGSVTKKMLPTGKPLDVIDGVETSCVDIAMPVVLMRAEAFGKTGHETAVEL
                     DADRELIKRMEAIRVKAGVLMGMGDVSKSVVPKMALLSKARNGGVIASRFFVPETCHK
                     AHPVTGTVCIASACAIPGTVASQIATLPPAPQGMIRIEHPQGMIIIDLDVDFTNQQQV
                     LRRAALIRTARRIFEGSVCVPEKVWAGKRVPQPATATA"
     gene            3145..4434
                     /gene="hpnE"
                     /locus_tag="AC158Q_10475"
     CDS             3145..4434
                     /gene="hpnE"
                     /locus_tag="AC158Q_10475"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HIR3340241.1"
                     /translation="MNVAVIGGGYAGMAAAVTLAERGVPVTVFEAAKTLGGRARRVEH
                     RDLALDNGLHILIGAYGETLRLMRQVGADPDQLLLRLPLSWRIHDRFSFQAARLPAPL
                     HLLAGLLAARGVPVLERLAAVRFMLRMKWQGYVLPHDTTVDILLECEGQGEGLTQLLW
                     QPLCVSALNTPPAAASAQVFLNVLRDSLDADRAASDMLLPRADLSRLFPEPAADYVRA
                     NGGEVLTGRRVTGIDEHGGSFMVRSADDGRPYSHVVCALPPYHAGAFLIGISALAETA
                     EAAERLAYQPIYSVYLRYPAEVRLPAPMLGFDSPLLQWAFDRGALSGQHGVIGAVISA
                     EGSHQNLSQDELGRLVHQELQQQVGLLPDPLWCRVIAEKRATFACTPDLKRPHARTAL
                     RNFLLAGDYVASDYPGTIESAVRSGVSAARMIVDSKA"
     gene            4655..6286
                     /locus_tag="AC158Q_10480"
     CDS             4655..6286
                     /locus_tag="AC158Q_10480"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HIR3340242.1"
                     /translation="MQYDVVIVGGGPAGLAAAIRLKQLAAQHRHEVSVCVLEKGSEVG
                     AHILSGAVMDPRALDELFPDWRQRNAPLNAPVSEDRFLFLTEGASYKVPGFMLPGCFR
                     NHGAYVVSLANVCRWLAQQAEGLGVEIFAGFAAAEALTAENGAVRGVATGDMGIGRDG
                     KPGANHQRGMELHARYTFFAEGCRGHLGKLLQDRYNLRAGADPQVYGLGMKELWEIQP
                     ERHRQGLVIHTAGWPLATDTYGGSFLYHLENHQVSIGFVVGLAYSNPYLSPYEEFQRY
                     KTHAAIRPFLEGGKRIAYGARAIAAGGLQSLPKLTFPGGCLIGDDAGFLNASRIKGSH
                     AAMKSGMLAAETAFSALRAGRSHDELGAYPEAFRGSWLHDELHRARNFKPWMGKGLVL
                     GTLMVGVDQVLLRGQAPWTLHHGHADHETLKTKSEAEPIAYPKADGVITFDRLSSVFV
                     SNTNHAEDQPVHLTLKDASVPVKVNLELYDAPEQRYCPAGVYEIVRDADGRNPRLQIN
                     AQNCVHCKTCDIKDPTQNIVWVAPEGGGGPNYPNM"
     gene            6320..7882
                     /locus_tag="AC158Q_10485"
     CDS             6320..7882
                     /locus_tag="AC158Q_10485"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR3340243.1"
                     /translation="MNRILALFRLRWVQFLLTLLATAAIGGALVATIYLMEFSIMWIA
                     FLGGILFAAMVALASRASRAEWVIAERTVQVERLREQLTQEIARGRHAAEAMRLAEAR
                     LRLVSDGLPNPLLFIDRDQRCHYHNRAVERLSGLSSDKISGQFLRDIVGHTAYLSMAP
                     RIDEALSGRATEYELAWERPGAVSTNYTVRHIPYPAGELQPQGYYLLLTTAVTQSGAT
                     PAAAPRSAVPRPGSGGGAEAAAGTAGDTSQLGAEGGESLYLRSMTDDLMGWNDPRAKL
                     SRALEENQFLLFAQKILALKSGLADPSCMEILLRLQEEEDNFLPPGGFLPLAERYGMM
                     EDLDRWVVRNVISWCLKRMRKDPDSRLPLCCVNLSEASVKSLEFAPYVMNELQIAGLP
                     ARTLCFEIGEQDAINHHAYAQRLIAALKPAGCLHTIDAFGSTKVTFSYLKGLPIDFLK
                     IDGNIIQYILRDSAELAKAKAINTVCQKVGVRSIAEFVESKETLGKLREIGMDYVQGF
                     GIARPEPISKLS"
     gene            7939..8685
                     /locus_tag="AC158Q_10490"
     CDS             7939..8685
                     /locus_tag="AC158Q_10490"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding periplasmic protein"
                     /protein_id="HIR3340244.1"
                     /translation="MLNATAIARTACLLTGLLLASQPALALKLLTEENPPLNYTENKK
                     LTGMATEVVQEMGRRANMKLDFEVMAWEKAYEKAQADKETCLYSTARLSNRENAFKWV
                     GPIAVNKWGLYALSGFKPAIKSLKDVRPYRVGGVERDAKTEFLKQQGVTNITEERDDK
                     LNPAKLTLNRKEAQKIDLWITSVASARKVAAQAKVPGVKLVYVVREAESYLACSPRTA
                     AATLKSLAGALDGMKKDGSYQKIMKAYESQ"
     gene            complement(8812..>9361)
                     /locus_tag="AC158Q_10495"
     CDS             complement(8812..>9361)
                     /locus_tag="AC158Q_10495"
                     /inference="COORDINATES: protein motif:HMM:NF027363.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HIR3340245.1"
                     /translation="AGAWLLGGAAVAAEVEGVKLADKVRLGDGGPELVLNGAGLRTRV
                     FFKVYVGALYLREKSGSAQAVFADNGPKRVAMHMLRDLTADQLFSAFNDGLKANHAPA
                     EVAKLEPQVKQLEGIFSAVKAAKSGDTILLDYVPGAGTRVVVNGNARGTIAGEEFNRA
                     LLRIWLGDQPVDAALKKAMLGG"
BASE COUNT         1703 a         3110 c         2998 g         1550 t
ORIGIN      
        1 acccgccccc tcaccctttc cctctccccc ggtccggggg agagggaata tgtagtcgaa
       61 tcacgaatta caaatcacga gtcacgcccc gcccagtgat ggatccccac gactattgcc
      121 agaccaaagc cgccgccagc ggctcgagct tttattacag cttcctgttc ctgccgccgg
      181 aacgccggcg cgcgatcacg gcgctctacg cgttctgtcg cgaggtggac gacgtggtcg
      241 acgaatgctc ggacgcagcg gtggcgcgca tgaagctcgc ctggtggcgc aaggaagtgg
      301 cggccgccta tcacggcacg ccccagcatc ccgtggcgcg cgcgctgtcc gaggtcgcgc
      361 cgcgcttcaa tctctccgaa gaacgcctga acgagatcat cgacggcatg cagatggatc
      421 tcgattacaa ccgttatccc gatttcgaca cgctcaagct ctactgccac cgcgtggcca
      481 gcgtcgtcgg gctgctctcc gcggaaatat tcggctacca ggaccggcgc acactggact
      541 atgccgccga tctcggcatg gcgttccagt tgaccaacat catccgggac gtgggcgagg
      601 atgcccgccg caaccgcgtc tatctgccgc tcgacgagat cgcacgccac ggaatgagcg
      661 agtccgacat cctgcaggcg cgcgagaccg atgccttccg gcgcctgatg gaattccaga
      721 tccggcgcgc gttgggctat taccgggatg cgttcgccaa gctccccgcc ggcgaccggc
      781 gcgcgcagcg gccaggcctc gtcatggcgg cgatttacca gacgacgctc gacgaaatcc
      841 ggcgcgacgg ctgccgcgtg ctgacccagc gcgtttcgct cacgcccatc agaaagctgt
      901 ggatagcgtg gcgcacctgg gtcaagggct gaacgacgtc gcaggtagag cgtcgcagat
      961 agtttgttat ggcggtcacg aagagtaacg tcgcacgccg tatccgggac acttcaactg
     1021 tggcgcctca tctgagacgc ttcatctgag acgcactgaa tggacatacg aatatggacc
     1081 tgcaactgaa gggcaagacg gctctggtga cgggcgcaag catgggcatc ggccgcgcga
     1141 tcgcgaaggg gctggccgcg gaaggcgcaa ggatatgcat cgcggcgcgg cgcggaaatc
     1201 tgctcgagga actggcgaag gagatcgccg cggcgggcgg cgcccgtccc ggcatcgtcg
     1261 agatcgacgt gctgcccgag ggcgcgccgg cacgcctggc gcgcgccgcg cttgatgccc
     1321 tcgggcatat cgacatcctc gtgaactccg ccggcggcag caagcccgcg atcccgatcg
     1381 atgccccgga agaggcgtgg gaggagtcga tgacgctcaa tttcgtgcgc gtgcgccagc
     1441 tcacccacgc catgctcccc ggcatgatcg ggaaaaaatg ggggcgcgtc atcaacatca
     1501 gcggcaagtc ggagcccgag cggctgatcg ccgcgacgcc gaccaaggct gcgatccacg
     1561 cctggtccaa ggggctgtcg cgcgaggtcg gccgctacgg catcaccgtg aacagcattg
     1621 cgccggggcg catcatgagc gagcagatcc gccgcaagta ctccgaggag ttccgcgcga
     1681 aacagtccgc cgaggaaatc ccggtcggcc gctacggcga accggaagag ctcgccgcgc
     1741 tggcggtctt cctcgcctcc cccgtcgccg gctacatcac cggggcggtg ctgccggtcg
     1801 acggcgggct caggcgctac gctttctaac ggcgtcgcag atacgacgtc tcagatactg
     1861 cgtctcagat aaagcgtctc agatagtgcg ggcgagagac gcttcacctg ggacgcctta
     1921 cctgggacgc cttacctgag acgcctttcc tgagacgctg gtcttggtcg tataatccgg
     1981 ctagtttcgc aggagaatat tgatatgcaa accaagatcc catgcgtgat catgcgcggc
     2041 ggcacgtcgc gcggcccgtt cttcaagctg accgacatgc ccgccgacat cccgacccgc
     2101 gatgcggtgc tgctctcggt gatgggctcg ccgcaccaga tccagatcga cggcatcggc
     2161 ggctcgcact cggtgacgag caaggtcgcg atgatcagca aatcgaagcg tcccgacgcc
     2221 gacgtcgatt acctgttcgc gcaggtgcag atccacgaga gcatcgtgga caccaagccc
     2281 aactgcggca acatgctggt ggcggtcggc ccgttcgcta tcgatgcggg cctggtgccc
     2341 gcgaagaacg gcgagaccac ggtgcgcatc ttcaacgtca acacccagtc gctggtcgag
     2401 gcgatcgtcc agacccccgg cggccaggtc cgttacgacg gcgacgccgc catcgacggc
     2461 gtgccgggca cggcggcgcc ggtcggcatc aatttccaga gcgcgatcgg gtcggttacc
     2521 aagaaaatgc tgcctaccgg aaaaccgctc gacgtgattg acggcgttga aacgagctgc
     2581 gtggacatcg cgatgccggt agtcctgatg cgagcggaag cgttcggcaa gaccggccac
     2641 gaaactgccg tcgagctcga cgcggaccgg gaactcatca agcgcatgga ggcgatccgc
     2701 gtcaaggccg gcgtgctgat gggcatgggc gatgtctcga aatcggtggt gccgaaaatg
     2761 gccctgctgt cgaaggcgcg caacggcggc gttatcgcct cgcgcttctt cgtgcccgaa
     2821 acctgtcaca aggcgcaccc ggtaaccgga acggtgtgca tcgcctcggc gtgcgccata
     2881 cccggaacgg tggcctcgca gatcgccacc ctgccgccgg ccccacaggg gatgatcagg
     2941 atcgagcacc ctcaggggat gatcatcatc gacctggacg tcgattttac gaatcagcag
     3001 caggtgttgc gccgcgccgc gctgatccgc accgcgcgcc gcatcttcga aggcagcgtc
     3061 tgcgtgcccg aaaaggtctg ggccggcaag cgcgtcccgc agcctgccac cgcgaccgcg
     3121 tagcgcccgc cagccgcgcg cgacatgaac gttgccgtca tcggcggtgg ctacgccggc
     3181 atggcggcgg ccgttacgct cgccgaacgg ggggtgccgg tcacggtatt cgaggcggcg
     3241 aaaacccttg gcggccgcgc gcgccgggtc gagcaccgtg atctcgcgct cgataacggt
     3301 ctgcatatcc tcattggcgc ctacggcgag acgctgcgcc tgatgcgcca ggtgggcgcc
     3361 gatcccgatc agttgctgct gcgccttccg ctctcctggc gcatccacga ccggttctca
     3421 ttccaggccg cccgcctgcc ggcgccgctg cacctgctcg caggacttct tgccgcgcgc
     3481 ggcgtgcccg tcctggagcg cctggccgcg gtgcgtttca tgctgcgaat gaagtggcaa
     3541 ggctatgtcc tgccgcatga caccacggtc gatatcctgt tggaatgcga aggccagggc
     3601 gagggcctga cgcagctgct gtggcagccg ctgtgcgtct ccgcgctcaa caccccgccc
     3661 gcggcggcct cggcacaggt gtttctcaat gttttgcgcg acagcctcga cgccgatcgc
     3721 gcggcgagcg acatgctgct tccacgcgcc gatctctcgc gcctttttcc cgagcccgcg
     3781 gcggactacg tcagggcgaa cggtggggag gtgctgaccg gtcggcgggt gacgggaata
     3841 gacgagcacg gaggcagctt catggtacgc agcgcggatg acggtcgccc gtacagccac
     3901 gtcgtctgcg ccctgccccc ctatcatgcc ggggcgttcc tgatcgggat ttcggcgctc
     3961 gccgagacgg ctgaagcggc cgagcgcctt gcctaccaac cgatctactc ggtctacctg
     4021 cgctatccgg cggaggtgcg actgcccgct ccgatgctgg gtttcgacag cccgctgctg
     4081 cagtgggcgt tcgatcgcgg cgctctgtcc gggcagcacg gcgtgatcgg cgcggtgatc
     4141 agcgcggaag gctcccacca gaacctgtca caggacgagc tcgggcgcct ggtccaccag
     4201 gagctgcagc agcaagtcgg gctgctgcca gacccattgt ggtgccgggt catcgccgaa
     4261 aaacgcgcca ccttcgcatg cacgccggac ctcaagcggc cgcacgcccg cactgcgctc
     4321 aggaatttcc tgctcgcggg cgattacgtc gccagtgact atcccggcac gattgaatct
     4381 gcggtgcgca gcggagtcag cgctgcccgc atgatcgttg actcaaaagc gtgattcgtg
     4441 agagtcgtga ttcgccgatt gcaccacccc gcccgccgcg caggcatccc tcctcgacaa
     4501 ggaggggagt aaggtccagg cgaatcacaa atcaacggtg tcacgaatcg aatcacgact
     4561 cacgaatcac gcagttggag agcatgaggc cgtgccgcac tttataatgc cggaaagcat
     4621 caattgagga taagaagtgg ccgaacgcga atccatgcag tacgacgtcg tgatcgtggg
     4681 tggcggtccc gccgggctcg ccgcggcgat ccggctgaag cagctggcgg cacagcaccg
     4741 gcacgaggtc agcgtctgcg tgctggaaaa aggctcggag gtcggcgcgc acatactgtc
     4801 cggcgcggtg atggacccgc gcgcactgga cgagttgttc ccggactggc ggcagcgcaa
     4861 cgcgccgctc aacgcgccgg tctccgaaga ccgcttcctg ttcctcaccg aaggcgcctc
     4921 gtacaaggtg ccgggcttca tgctgccggg ctgcttccgc aatcacggcg cctacgtggt
     4981 gagtcttgcc aacgtgtgcc gctggctcgc gcagcaggcg gaaggattgg gcgtcgagat
     5041 cttcgccgga tttgcggccg cggaagcgct gacggccgag aacggcgcag tgcgcggcgt
     5101 cgccacgggc gacatgggga tcggacgcga tggtaaaccg ggcgccaacc atcagcgcgg
     5161 catggaactg cacgcccgct atacgttctt cgccgagggc tgtcgcggcc atctcggcaa
     5221 gctgctgcag gaccgctaca acctgcgcgc cggcgccgac ccgcaggtct acggcctcgg
     5281 gatgaaggag ctgtgggaaa tccagcccga gcggcaccgg caagggctcg tcatccatac
     5341 cgccggctgg ccgctcgcaa ccgatacgta tggggggtcg tttctctacc atctcgagaa
     5401 ccaccaggtc tcgatcggct tcgtggtggg gctcgcctac agcaacccgt acttgagccc
     5461 gtacgaggaa ttccagcgct acaagaccca cgccgcgatc cggcccttcc tcgaaggcgg
     5521 caagcgcatt gcctacggcg cgcgcgccat cgcagccggg ggcctgcagt cgctgcccaa
     5581 gctcactttt cccggtggtt gcctgatcgg cgacgatgcc gggttcctca acgcctcccg
     5641 catcaagggc agccacgccg cgatgaagtc ggggatgctg gctgcggaaa cggccttctc
     5701 cgcgctgcgc gcggggcgct cgcacgacga gctcggcgcc tatcccgagg cgttccgggg
     5761 aagctggctg cacgacgagc tccaccgggc gcgcaatttc aagccctgga tgggcaaggg
     5821 gctcgtgctc ggcacgctga tggtgggcgt ggaccaggtc ttgctgcgcg ggcaggcccc
     5881 gtggacgctg caccacgggc acgccgacca cgagacgctc aagaccaaat cggaagcgga
     5941 gccgatcgcg tatcccaagg cggacggcgt gatcactttc gaccgcctgt cctcggtctt
     6001 cgtctccaac accaaccatg ccgaggacca gcccgtccac ctcacgctca aggacgcatc
     6061 ggtccccgtc aaggtcaacc tcgagctcta cgacgccccc gaacagcgct actgcccggc
     6121 cggggtctac gagatcgtgc gcgatgccga cggcaggaac ccgcggctgc agatcaacgc
     6181 ccagaactgc gtgcactgca agacctgcga catcaaggac ccgacgcaga acatcgtgtg
     6241 ggtcgcgcct gaaggcggcg gcggccccaa ttatcccaac atgtaggcac ggccgcgccc
     6301 gggttaatcc tccctgccca tgaaccgcat actcgcgttg ttccgcttgc gctgggtcca
     6361 gttcctgctg accctgctgg ccacggcggc catcggcggc gccctggtcg cgaccatcta
     6421 cctgatggag ttcagcatca tgtggatcgc gtttctcggc ggcatactgt tcgccgccat
     6481 ggtcgcgctg gcgagccgtg cctccagggc ggaatgggtg atcgcggagc gcacggtgca
     6541 ggtcgagcgg ctgcgcgagc agttgacaca ggaaatcgcg cgcggtcgcc atgcggccga
     6601 ggcgatgcgc ctggcggaag cgaggttgcg gctggtctcc gatgggctcc cgaatccgct
     6661 cctgttcatc gatcgcgacc agcgctgcca ttaccacaac cgggcagtcg aacggctgtc
     6721 cggcctgagc tccgacaaga tcagcggcca gttcctgcgc gacatcgtcg gccacaccgc
     6781 ttatctcagc atggcgccgc gcattgatga ggccctgtcc ggccgggcta cggagtacga
     6841 gctggcatgg gagcgcccgg gcgcggtgag cacgaactac acggtccggc acattcccta
     6901 tcccgcgggc gagctgcaac cgcaaggtta ttacctgttg cttacgactg ccgtcacgca
     6961 atccggcgcc acgcctgcgg ccgcgccgcg gtctgcagtt ccgcgcccgg gttccggtgg
     7021 cggcgcggaa gcggcggccg ggacggcagg cgatacgtcg cagcttgggg cggaaggcgg
     7081 agagtccctg tatctgcgct cgatgaccga cgacctgatg ggctggaacg atccgcgcgc
     7141 aaagctctcc cgcgcgctgg aggagaacca gttcctgctg ttcgctcaaa agatcctggc
     7201 attgaagagc ggtctggccg acccgtcatg catggaaatt ctgctgcgcc tgcaggaaga
     7261 agaagacaac ttcctgcccc ccggcggctt cctgccgctc gccgagcgct acgggatgat
     7321 ggaggatctc gaccgctggg tggtgcgcaa cgtgatctcc tggtgcctga agcgcatgcg
     7381 caaggatccg gacagccgtc tgccgctctg ctgcgtgaac ctgtccgaag cgtcggtgaa
     7441 aagcctggag tttgcgccct acgtcatgaa cgagctgcaa atcgccggcc tgcccgcgcg
     7501 cacgctttgc ttcgagatcg gcgaacagga cgccatcaac catcatgcgt atgcgcaaag
     7561 gctgattgcc gcgctcaagc ctgccggctg cctgcacacc atcgatgcct ttggcagcac
     7621 caaggtgaca ttctcatacc tcaagggtct gccgatcgac ttcctcaaga tcgacggcaa
     7681 catcatccag tacatcctgc gcgactccgc cgagcttgcg aaagccaagg cgatcaatac
     7741 cgtctgccag aaagtcgggg tacgcagtat cgccgagttt gtcgagagca aggagacgct
     7801 cggcaagctt cgcgagatcg gcatggatta cgtgcagggc ttcggcatcg cgcggcccga
     7861 gccgatcagc aagctttcct gaacgggccc gctgtggcat tcttggcact tgtcacgccc
     7921 tgaatgggag gagaaaccat gcttaatgcg accgcgatag cccgtaccgc gtgcctgctg
     7981 accgggttgc tgcttgcatc ccagcccgcg ctggcattga aactccttac cgaggaaaac
     8041 ccgcctctca actacaccga gaacaagaag ctgaccggca tggcgaccga ggtcgtgcag
     8101 gaaatgggca ggcgcgccaa catgaagctc gacttcgagg tcatggcctg ggaaaaggcc
     8161 tatgaaaagg cccaggccga caaggaaacc tgcctctact cgaccgcgcg cctgtcgaac
     8221 cgtgaaaacg ccttcaagtg ggtcggccca atcgcggtca acaagtgggg gctctacgcg
     8281 ttgagcggat tcaagccggc gatcaaatca ctgaaggacg tgaggcctta ccgcgtgggc
     8341 ggggtcgagc gcgacgccaa gaccgagttc ctcaagcagc agggcgtcac caacatcacc
     8401 gaggagcggg acgacaagct caatcccgcg aagctcacgc tgaaccgcaa ggaagcccag
     8461 aagatcgatc tctggatcac cagcgtcgct tccgcgagga aagtcgcggc ccaggccaag
     8521 gtgcccggcg tcaagctggt ctacgtcgtg cgcgaggcgg agtcctacct ggcatgcagc
     8581 ccgcgcaccg cggcggccac cctcaagtcg ctggcgggcg cgctggacgg catgaagaag
     8641 gatggaagct accaaaagat catgaaagct tacgagagcc aatagcccgg tcccgctaac
     8701 tgcgtgattg gtgaatggtg aatgggctag agccgtttcg cgaaacgggg tccgggagtc
     8761 aggccactca atattcacca ctcgccgttc acgactttcc ggccatcggc gtcagccgcc
     8821 gagcatcgcc ttcttgagag cagcgtcgac gggctggtca cccagccaga tcctcagcag
     8881 cgcgcgattg aattcctcgc ccgctatcgt ccccctggcg ttcccgttga ccacgacccg
     8941 cgtccccgct cccgggacgt aatcgagcag tatcgtatcg ccgctcttcg cggccttcac
     9001 cgcgctgaat atcccctcga gctgcttcac ctgcggctcg agttttgcaa cttcggcggg
     9061 cgcatgattg gccttgaggc cgtcgttgaa cgcggagaac aattgatccg ccgtgaggtc
     9121 gcgcagcatg tgcatggcga cgcgcttggg gccgttgtcg gcgaatacgg cctgcgcgct
     9181 tcctgacttc tcccgcaggt agagcgcgcc gacgtagacc ttgaagaata cgcgcgtacg
     9241 caagccggca ccgttcagca ccagttcggg cccgccgtcg cccagccgta ccttgtcggc
     9301 gagcttcacg ccttctacct cggccgcgac ggccgcgccg cccaacagcc aggcccccgc
     9361 g
//
LOCUS       DBNGSG010000405        11013 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1148924, whole genome shotgun sequence.
ACCESSION   DBNGSG010000405 DBNGSG010000000
VERSION     DBNGSG010000405.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 11013)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11013)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11013
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1148924"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1763)
                     /gene="glnE"
                     /locus_tag="AC158Q_10500"
     CDS             complement(<1..1763)
                     /gene="glnE"
                     /locus_tag="AC158Q_10500"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HIR3340246.1"
                     /translation="MESHNAGASPSADTLASALLARAARLSHYARRLVEAAPRSELDT
                     AVGQPFSADEMRAALAADPGRDEAGLKRALRLLRARVMLRLIARDLGGRAPLGEVMTT
                     ASALAEVALAHALARLDGWLADRHGRPVGAVSGREQQLHVLGMGKLGGAELNVSSDVD
                     LVFVYPEEGETRGPQPISNHEYFTRLGKQLIAALSEITADGFVFRVDMRLRPYGDAGP
                     LAASFDALENYFITQGREWERYAWIKARALTGDREPELMELVRPFVFRRHLDFNAIAA
                     LRDLHRQIRLEVERRDLFDNIKLGPGGIREIEFVTQVFQLIRGGRDPDLGQPPTLAVL
                     PLLARRNLLPATAVRELTEAYCFLRSLEHRLQYLDDQQTHVLPRSDADRALVAEAMNC
                     DNYAALAAALEQHRGRVTRHFEDIFAASTDGEHSLARLWQENPDTDSSVNTLAALGFR
                     QPREIGSRLEAMRAGSRYREMPAASQARLDRLVPLAVEVAASQKDPDTTLDRVLDLLE
                     SIARRGSYLALLVEYPQALSRLAAMMSASPWVAQYLTGRPILLDELLDTRTLYAAPDW
                     PAAARQLRAQVDDAADDTEKQ"
     gene            1889..5662
                     /locus_tag="AC158Q_10505"
                     /pseudo
     CDS             1889..5662
                     /locus_tag="AC158Q_10505"
                     /inference="COORDINATES: protein motif:HMM:NF024516.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
     gene            5674..6006
                     /locus_tag="AC158Q_10510"
     CDS             5674..6006
                     /locus_tag="AC158Q_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340247.1"
                     /translation="MLRFLLGAGYAAAVSAAAAAQIFVVPPELWDRPRNGRAVIEQPA
                     IRQAVNACLAEPGARLVVRHGAGQDSLLAAEELRSWLVALALEPGRISLRNDLQPSEP
                     LRLEVIRD"
     gene            6178..7053
                     /locus_tag="AC158Q_10515"
     CDS             6178..7053
                     /locus_tag="AC158Q_10515"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006497498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HIR3340248.1"
                     /translation="MNAARSSFGKAKPHIAKADGQGAVTRIAAVQLASGPNVKGNLQE
                     AGRLIEMAAAQGAKLVALPEHFAIMGLRDTDKVKAREKPGAGPIQEFLSATARRLGVW
                     IVGGSAPLESSNPDKVRNSSLVYDDKGKLAARYDKIHLFGFEMGSERYEEERTIEPGR
                     DVVTVDTPFARLGLSVCYDLRFPELYRAMKEVDIILVPSAFTETTGKAHWETLIRARA
                     IENLAYVLAPAQGGYHLSGRETHGDSMIVDPWGVVLDRLPRGSGVVGAGVNLQHLRNL
                     RKSLPALTHRTLHRC"
     gene            7398..8891
                     /gene="tldD"
                     /locus_tag="AC158Q_10520"
     CDS             7398..8891
                     /gene="tldD"
                     /locus_tag="AC158Q_10520"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HIR3340249.1"
                     /translation="MKPEDRQETRSLERETSFATANEVLLAPYSLDTSRLQTVFGQIF
                     AHRVDYADLYFQYTRSESWSLEEGIVKSGSFNIDQGVGVRAVSGEKTAFAYSDDISLP
                     ALEAAAQATRAIARQGQAGRTQVAKRATGGSLTGHNLYLPQDPIASLRDPAKVTLLER
                     IERRARALDPRVTQVMASLAGEYEVVLVARSDGVLAADVRPLVRLSLQVIVEQDGRRE
                     QGSAGGGGRFDYAYFTDDVLADYAKKAVDQALVNLDARPAPAGTMTVVLGPGWPGVLL
                     HEAIGHGLEGDFNRKGTSAFSGRIGERVAAPGVTVVDDGTIARRRGSLNVDDEGHPTQ
                     CNTLIENGVLKGYMQDSLNARLMKVAPTGNGRRESYAHIPMPRMTNTYMLNGDKPREE
                     IIASVKNGLYAVNFGGGQVDITSGKFVFSASEAYVIENGKITYPVKGATLIGNGPDAL
                     TRVAMIGNDMALDPGVGTCGKEGQSVPVGVGQPTMRIDGLTVGGTAS"
     gene            9068..10138
                     /gene="aroG"
                     /locus_tag="AC158Q_10525"
     CDS             9068..10138
                     /gene="aroG"
                     /locus_tag="AC158Q_10525"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HIR3340250.1"
                     /translation="MQFRTDDIRIREIKELVPPSHVLREFAISEKAARTVYETRQAIH
                     RILHGADDRLLVIMGPCSIHDVKAAKEYAGKLKREKEKLADDLLVVMRVYFEKPRTTV
                     GWKGLINDPNLDGSFRINEGLRIARRLLLDLDEMGMPVGCEFLDMITPQYIADLVAWG
                     AIGARTTESQIHRELASGLSCPVGFKNGTDGTVKIAVDAIRAASAPHHFLSVTKAGHS
                     AIVSTAGNEDCHIILRGGNKPNYDAANVDAAAKSLAEAGVPARLMIDFSHGNSSKKPE
                     KQVEVAQDVAGQLAAGEARIFGIMAESHLKAGRQDLLPGKELVYGMSITDGCIGWEDS
                     RKVLAMLADGVRQRRVRAQQAE"
     gene            10395..10940
                     /locus_tag="AC158Q_10530"
     CDS             10395..10940
                     /locus_tag="AC158Q_10530"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR3340251.1"
                     /translation="MALATKHEAGQGHLQHLEQVGKGAAIANEIFGLVGRSQFFAEFS
                     REDISVLAGYMDVYRAQPGETIIREDDGGDFMLLVIEGAVDIFKKGMRGEQQHMTNVG
                     PGMTLGEMSMIDGEPRFATCIATEPTVFAVLHREDMAKIILDHSSLGSKILVKLVSML
                     SARLRQTSARLLQYMERSSLV"
BASE COUNT         1981 a         3510 c         3740 g         1782 t
ORIGIN      
        1 atctgcttct cggtgtcgtc ggcggcgtca tccacctggg cgcgcaattg ccgcgcggcg
       61 gccggccagt ccggcgctgc gtagagcgtg cgggtatcga gcagttcgtc cagcaggatc
      121 gggcggccgg tcaggtactg ggcaacccac gggctcgcgc tcatcatcgc ggcgagccgg
      181 gacagggcct gcggatactc gacgagcagc gcgagatagg agccccggcg cgcgatgctc
      241 tccagcaggt cgagcacgcg gtccagtgtc gtgtcgggat ccttttgcga cgcggcgacc
      301 tcgaccgcga gcggcaccag ccggtcgagc cgcgcctggc tggctgccgg catttcgcga
      361 tagcggcttc ccgcgcgcat ggcctcgagc cggctgccga tctcgcgggg ctggcggaag
      421 ccgagcgcgg ccagcgtgtt gaccgacgaa tcggtgtccg ggttctcctg ccacaggcgc
      481 gcgagcgaat gctcgccgtc ggtggaggcg gcgaagatat cctcgaaatg ccgggtgacg
      541 cggccgcggt gctgctcgag cgctgccgcc agcgcggcgt aattgtcgca gttcatcgcc
      601 tcggcgacga gcgcgcgatc ggcatccgat ctcggcagca cgtgcgtctg ctggtcgtcg
      661 aggtactgaa ggcggtgctc gaggctgcgc aggaagcagt aggcctccgt cagttcccgc
      721 accgccgtgg ccggcaggag attgcggcgc gcgagcaacg gcagcacggc cagggtgggc
      781 ggctgtccca gatcgggatc acgcccgccg cgaatcagct ggaatacctg cgtgacgaat
      841 tcgatttcgc ggatgccgcc agggccgagc ttgatgttgt cgaaaaggtc gcgtcgctcc
      901 acctcgaggc ggatctggcg gtgcaggtcg cgcagcgcgg cgatcgcgtt gaaatcgagg
      961 tggcgcctga atacgaacgg acgtaccagt tccatcagct cgggttcgcg gtcgccggtc
     1021 agcgcgcgcg ccttgatcca ggcgtagcgc tcccattccc gcccctgggt gatgaagtag
     1081 ttctccagcg cgtcgaagct ggcggcgagc ggcccggcgt ctccgtacgg ccgcagccgc
     1141 atgtccacgc ggaacacgaa gccatccgcc gtgatctcgg ataacgcggc gatcagctgc
     1201 ttccccagcc gggtaaagta ctcgtggttg ctgatcggct gcggcccgcg cgtttcaccc
     1261 tcttccgggt agacgaagac gagatcgaca tcggaggaga cgttcagctc cgcgccgccc
     1321 agcttgccca tcccgagcac gtgcaactgc tgctcgcgcc cgctgaccgc gccgaccggc
     1381 cttccgtgcc ggtcggcaag ccacccatcg agacgggcga gcgcatgggc aagcgccact
     1441 tcggcaagag ccgaagccgt cgtcatcact tcgcccaggg gggcgcggcc tcccaggtcg
     1501 cgcgcgatca ggcgcagcat cacgcgcgcg cgaagaaggc gcaatgcgcg cttgaggccg
     1561 gcctcgtcac ggccgggatc agccgcgagc gccgcgcgca tttcatcggc gctgaacggt
     1621 tgcccgaccg cggtatccaa ctccgagcgg ggtgcggctt cgacgagccg gcgggcgtaa
     1681 tgactcagcc gcgcggcacg cgccaacagg gccgacgcga gcgtgtcggc ggatggcgac
     1741 gcgccggcgt tatgggattc catggtcgat tgcaggtaga atgcgtaagc ccttgactca
     1801 atatcgcatt ttttcgaggc ccaagcaaaa cgtggcggca gcggacaagc tcggatgggt
     1861 ggggatcagc tcgctctggg cctaccgcgt gctcacctgg gccgtgctgg cggcgggaat
     1921 gatcttcgcc acgatcgtga tctcgctgcg ctactggatc ctgcccgata tcgagagcca
     1981 tcgcgagagc atcgcgcgca tcgtcagcga gcgggcgcgg cagaaggtca cgatcgagag
     2041 gattcgagcc aactgggacg ggctgcgccc gcagctcaag ctggagaacg tcacggtgta
     2101 cgacgccacg ggcaggccgg cgttcaggct ctcgcgcatg gaccagacgc tgtcgtggct
     2161 ctcgctcatc acgttcgagc tgcgcttcca tgcgctcgac attcaccagc ccatgctcaa
     2221 catccggcgc gacgagaaag gaacgatttc gatcgcgggc gtcgagttga ccgggggcgg
     2281 gggcgacccc ggttttgcgg actggctgct gcgccagcgc gacatcgagg tgcacgatgc
     2341 cacgatcatc tggaatgacg atcagcgcaa ggcgccccag ctggaactga agagcgtctt
     2401 cctgcatgtg atcaacaggg gcggccggca ccgcttcggc ctgcgcgcgg tgccgccgca
     2461 ggatctcgcg gcgccgctcg acgtgcgcgg ggacctgacg ggcgatacgg tcaagtcgct
     2521 tgcggactgg gacgggcggc tgtacctcca gctcgagtct gccgatatcg ccgcctggcg
     2581 cacctggatt ccgtttccgg tcgaattccc gcgcggcgcc ggcgcggtgc gcgcctggct
     2641 cgcattcagc cagaaccggc tggtcgatgt catcgcggac gtgaagctcg ccaacgtgcg
     2701 cacccggctg gcggcggatc tgcccgaact cgacctcgcg gatctcgagg gacgcatcgg
     2761 ctggaaacaa tcggacactg gtttcgagat cacgacttcc cggctgggat tgaccaccag
     2821 cgggggtctt gccctccacc ccgcggattt tctgctgcgg gtgaccgcgg gcagcgcgca
     2881 ccggcaggga cgcggtgaaa tgcaggccaa tgcgctcgaa ctggcgccgc tgacgctgct
     2941 cgccgatcac ctgccgctcg cgccggaagt tcgccgggaa ctggcggcgt actcgcccgg
     3001 gggcagcttg aacgacttgc tggtgcggtg gaccggggag tggcacgagc cccagcagta
     3061 cagcgtccgc ggccggtttc agaatctctc gctcaaccgt tcgggcaagg taccggggtt
     3121 ctccggcgtc agcgggatgc tggagggcag cgagcgcggc ggcaccctgt cgctcaactc
     3181 gcacaagtca acggtggaaa tgccgctcgt tttccgcgaa ccgctcgaat tcgaatcatt
     3241 cgccgcgcag gtcgcctggg cgcgcagcgg cgggcagacc gagttgcggc tgaacagcat
     3301 ctcgttttcc aatgcgcatc tcgcggggac ggttttcggg aactaccggg tcgcgggcgc
     3361 cgcacgcggc agcatcgacc tgaccggcaa cctcacgcgg gcagatgccc gctacgtcag
     3421 ccgctatata ccgctggtgg tgggcaagaa tgcccgcgac tggctggaca aggcattcat
     3481 cgcgggccag tcgaacgacg tgacgctgcg cgtgaggggc gatctcgaaa agtttccgtt
     3541 tcccgacggc aagggcggcg tgttccaggt gaccgcgaaa gtcacggggg gcgtgctgca
     3601 ctatggcgat ggctggccgg acatcgaggg tattgccggc gatctcgtct tccgcggcaa
     3661 tcgcatggac gtgcatgcgc gcgccggcgc aatcctgggg gcgcgtatcg gcaagacgca
     3721 cgtcgagatt ccggacctgc agcacccgga gtcggtgctc aatatcaatg gagaggcgga
     3781 ggggcccacc ggcgaatttc tggcttacat cgaaaagagc ccggtgctgg acatggtcga
     3841 ccatttcacc aagggctggc aggtgcaggg aaacggcaag ctgtcgctga agctcgcgat
     3901 tcctctgcgc ggttcggcga agccccgggt cgcgggggct taccagttcg ccgataacac
     3961 cgttgccatc tcctccgagc tgcccaccgt ggaacaggcg ggcgggcggg tcgagttcac
     4021 cgattcgagc gtgcgcgcgc aaaacgtgag cgcggtcgtg ctcggcgggc cggtgaccat
     4081 ttccgcggcg acctcgcgcg atgccgccgt gcgcatcaac ctgcaggggc gcgtcaatgc
     4141 cgacagcgcg aggcgcacca acggccctgc atgggtacag cacctgcgcg gctctaccga
     4201 ctggcgcgcc gtgatcaatg cgcgcaagcg caatgcggac gtcgtgttcg aatcgagcct
     4261 gcaggggctt gcggtcgacc tgccggcgcc ggtcgtgaag accgcagccg aggccatgcc
     4321 gctgcgcatc gagcgtcgcg tgctggcggc gaaccaggac cgtctgagcc tgtcgctggg
     4381 cgatatcgtg agcatgaacc tggtgcgccg gaccgatggc gagcgcgccg ccattacgcg
     4441 cggttccgtg cgcctgggcg ccgcggccgc cgagcccgac cgcaacggcg tgtgggtgag
     4501 cggcgcggtg aaaaccttcg acctggaccg ctggcttgcc ttcatcgggc agggcgatgg
     4561 cgacacccga atcgaatggg gcggcgtgga tgtcaagttc ggaacggtgg acctgttcgg
     4621 ccggcgcttc agcgagctgg cggtcggcgg cgccacgcac gacgggcaat ggcgcggcaa
     4681 ccttgtcggc taggaactcg aggggacgct gctctggcag ccccagggcc gcggcaggct
     4741 cgtggcgcgc atgaaaacgc tcaccattcc ggcggccgcg tccggcacgg tgccggcgga
     4801 ggaagccgcc ctgcgcaaaa aggatgtgga actgccggcg ttcgacgtta ccgccgagca
     4861 gttcatcaac aaggacaagc tgctggggcg gctcgaactg gctgccgtgc cggaggggcg
     4921 cgactggcgc atcgagcggc tgcgcatcgt caatcccgaa gccacgttca cgctcgacgg
     4981 cgtctggcaa ctcgggaatg cgccgccgcg cacgcaggtc aatctgaagc tggatgcgtc
     5041 cgatgccggc aagttgctca cccgcctggg ctatcccgag ggcgtgcggc gcggtaccgc
     5101 caagctggaa ggcgctctgt cgtggaacgg ggcgccgtac gatctcgact acccctccct
     5161 ctccggcaat gtggtgctgg aagcgtccaa gggccagttc gtcaagctcg agccggggat
     5221 cggcaagctt ctcggcatac tcagcctgca gtcgctgccg cgccgcatct cgctggattt
     5281 ccgcgacgtg ttcagcgaag gcttttcatt cgacgagatc gtgggtgccg caaggatcag
     5341 ccgcggcatc gccagcaccg agaactttcg catccagggg ccgcccgcac gcatcacgat
     5401 gagcggggaa gtggacctcg cgcgcgaaac ccagaatctg aggatgcgca tcacgccgca
     5461 cgtgagcgat tccgtgtcca ttgcgggcgc gctcgttggc ggcccgatag ccggcgtcgc
     5521 cgcgtttctc gcgcagaaga tcctgaagga tccgctcgat caggccattt cctacgagta
     5581 caacgtgacc ggaacgtggt cggagccgaa cgcgatccgg atcgcgcggc cggccgccag
     5641 cgacgaagcc gcgcagccat gagcggcggg gtgatgttaa gatttctcct gggcgcggga
     5701 tatgctgcgg cggtgtccgc tgccgcggca gcgcagatct tcgtggtgcc ccccgagttg
     5761 tgggaccggc cgcgcaacgg ccgcgccgtg atcgagcaac cggcgatccg gcaggcggtg
     5821 aacgcctgcc tggctgagcc gggcgcgcgc ctggtcgtgc gccacggtgc gggccaggat
     5881 tcgctgctcg ccgccgagga attgcgctcg tggctggttg cgctcgcgct cgagcccggg
     5941 cgcatctcgc tgcgcaacga tttgcagcca tccgagccgc tgcggctcga ggtgattcgt
     6001 gactgatgaa cgcagaaaaa tcgaaacaag aatagacagg attaacaagc gacgagtccc
     6061 cccatcctac cttcccccgc ttgcggggga aagggataaa caagattatt gaatagatct
     6121 aatcctgtga atcttgttaa atcttgtgaa tcctgtccat tttttgattc ttgagtcatg
     6181 aacgcggcgc gttcctcctt cggcaaggcc aagccgcaca ttgcgaaagc cgacgggcag
     6241 ggcgcggtga cgcgcattgc cgcggtgcaa ctcgcctccg gacccaacgt gaagggcaat
     6301 ctccaggagg cgggcaggct gatcgagatg gcggcggcgc agggcgcgaa gctcgtagcg
     6361 ctgccggagc atttcgcgat catgggcctg cgcgataccg acaaggtcaa ggcgcgcgag
     6421 aagcccggcg cgggcccgat ccaggaattt ctgtccgcga cggcgcgccg gctcggcgtc
     6481 tggatcgtcg gcggctcggc gccgctcgaa tcctcgaatc cggacaaggt gcgcaacagc
     6541 agcctggtct acgacgacaa gggcaagctg gccgcgcgct atgacaagat ccacctgttc
     6601 ggcttcgaga tgggtagcga gcgctacgag gaagaacgca ccatcgagcc gggccgggac
     6661 gtggtgacgg tggacacgcc attcgcgcgc ctggggctgt ccgtctgtta cgacctgcgt
     6721 tttcccgagt tgtaccgcgc gatgaaggag gtggatatca tcctggtgcc ttcggctttc
     6781 accgaaacga ccggcaaggc gcactgggag acgctgatcc gggcgcgcgc gatcgagaac
     6841 ctcgcttacg tgctggcgcc ggcgcagggc ggctatcacc tgagcgggcg cgagacccac
     6901 ggcgacagca tgatcgtgga cccctggggc gtggtgctcg atcgcctgcc gcgcggctcg
     6961 ggcgtggtgg gggccggggt caacctgcag cacctgcgca acctgcgaaa gagcctcccg
     7021 gcgctgacgc accggacgct gcaccgctgc tgatgaaaat ggacaggatt gacagaatcc
     7081 ctcaaaaacc cgccacaccc gtcaacaccg taaatggaca ggattaacag ggattggtcg
     7141 caaaacccgt caacacctct aatagacacg attaacagga ttaaagcgat tagcagagct
     7201 cagaaagcct gttttttgaa tcttgttaat cttgttaatc ctgtccatta atgcttttgg
     7261 cgggtttttg aatcgtttag ttaatcctgt ctattaatgg ggttaacggg ttttcttgta
     7321 tgagttctaa ttccgtaaat cctttctgtt gttttgcttg aacttgctcc gtccgccgtc
     7381 atctatactg gatagtcatg aaacccgaag accgccagga aacgcgcagc ctcgagcgcg
     7441 agacttcgtt cgccaccgcg aacgaagtcc tgctggcgcc gtactcgctt gacaccagcc
     7501 ggctgcagac agtgttcggg cagatattcg cgcaccgggt ggactatgcc gacctctact
     7561 tccagtacac gcgcagcgag tcctggagcc tggaggaggg catcgtcaag tccggcagct
     7621 tcaacatcga ccagggcgtc ggcgtgcgcg cggtgagcgg cgagaagacc gcgttcgcct
     7681 attccgacga catcagcctg cccgcgctgg aagccgcggc ccaggcgacg cgggcgatcg
     7741 cgcgccaggg gcaggccggc cgcacccagg tggcgaagcg cgcgacgggg ggcagcctca
     7801 cgggtcacaa cctgtatctg ccgcaagacc cgatcgcgag cctgcgggac ccggcaaaag
     7861 tcaccctgct cgagcgcatc gagcgccggg cgcgcgcgct cgacccgcgc gtgacgcagg
     7921 tcatggcctc gctcgccggc gagtacgaag tggtgctggt tgcccgctcg gacggcgtcc
     7981 tcgccgccga cgtgcgcccg ctggtgcggc tctcgctgca ggtcatcgtc gagcaggacg
     8041 gccgccgaga gcagggcagc gcaggcggcg gcgggcgctt cgactacgcc tactttaccg
     8101 acgacgtgct cgcggactac gcgaagaaag cggtggacca ggcgctggtc aatctcgacg
     8161 cgcggccggc gccggccggc accatgacgg tggtgctggg cccgggatgg ccgggcgtgc
     8221 tgctgcacga agcgatcggc cacgggctgg aaggcgactt caaccgcaag ggcacgtcag
     8281 cattcagcgg tcgcatcggc gagcgggtgg cggcgcccgg cgtcaccgtg gtggacgacg
     8341 gcacgatcgc gcgccggcgc ggctcgctca acgtcgacga cgaggggcac cccacgcaat
     8401 gcaacacgct gatcgagaac ggcgtgctga agggctacat gcaggacagc ctgaacgcgc
     8461 gcctgatgaa agtcgcgccc accggaaacg gccggcgcga gtcgtacgcg catatcccga
     8521 tgccgcgcat gaccaatacc tacatgctga acggcgacaa gccgcgcgag gaaatcatcg
     8581 cctccgtcaa gaacggcctc tatgccgtca acttcggcgg cggccaggtg gacatcacca
     8641 gcggcaagtt cgtgttctcc gcttccgagg cctacgtgat cgagaacggg aagatcacgt
     8701 accccgtaaa gggcgcaacg ctgatcggca acgggcccga cgccttgacg cgcgtcgcaa
     8761 tgatcggcaa cgacatggcg ctcgacccgg gcgtgggcac ctgcggcaag gaaggccaga
     8821 gcgtgccggt cggcgtcggg cagcccacca tgcgtatcga cggtctcacc gtcggcggca
     8881 ccgcctcgta acccgtgaat cgggattcgt gaatcgagag tcgtgagtcg tgaatcgcga
     8941 aagcgattca cagttcggtt gtgggccgaa tcacaaatca cgaatcacca atcacgccgt
     9001 ttgtgccccg cttcgccccc acctcgcgtc ctgctataat gctttgattt atcagatgaa
     9061 tggcgccatg caattccgca ccgacgacat ccggatcagg gaaatcaagg agctcgtgcc
     9121 gccgtcgcac gtgctgcgcg agttcgcaat ttccgagaag gccgcgcgaa cggtctacga
     9181 gacgcgccag gcgatccatc gcatcctgca cggcgccgac gaccggctcc tcgtcatcat
     9241 ggggccgtgc tcgattcatg acgtgaaagc ggcgaaggag tacgccggga agctgaaacg
     9301 cgagaaggag aagctggcgg acgatctgct ggtggtgatg cgcgtctatt tcgagaagcc
     9361 gcgcacgacc gtgggatgga aggggctcat caacgacccg aacctcgacg gcagcttccg
     9421 catcaacgag gggctgcgca tcgcgcgccg cctgctgctc gatctcgacg agatgggcat
     9481 gcccgtcggt tgcgagttcc tcgacatgat cacgccccag tacatagccg acctggtggc
     9541 gtggggcgcg atcggcgcgc gcacgaccga gtcccagatc caccgcgagc tcgcatccgg
     9601 actgtcgtgc cccgtcggct tcaagaacgg caccgacgga accgtcaaga tcgcggtgga
     9661 tgcgatccgc gcggcgagcg cgccgcacca tttcctgtcg gtcaccaagg ccggacacag
     9721 cgcgatcgtg tccaccgccg gcaacgagga ttgccacatc atcctgcgcg gcggcaacaa
     9781 gccgaactac gatgcggcga acgtggacgc cgccgcgaaa tcgctggcgg aagccggcgt
     9841 gcccgcgcgg ctcatgatcg acttcagcca cggcaactcg agcaagaaac ccgagaaaca
     9901 ggtggaggtg gcgcaggacg tggcgggcca gctggccgca ggagaagcgc gcatcttcgg
     9961 catcatggcc gaaagccacc tcaaggccgg ccggcaggac ctgcttcccg gcaaggaact
    10021 ggtctacggc atgagcatta ccgacggctg catcggctgg gaggacagcc gcaaggtcct
    10081 cgccatgctg gccgacggcg tgcgccagcg gcgggtcagg gcgcagcagg ccgagtgacc
    10141 gcagagcggt cactgaggga tgagggcgga gggcggaggg atgagggcaa gaagtgtcga
    10201 ggattgagtg tcgagtgagc aatgcgtaac agggttttta ccccccgaca ctcgacactc
    10261 gacacccgac ccccgacccc gatactcgta acacgacaca cgattcacgc ttttggataa
    10321 ttagctggaa cgccgccgct gtaatatagt ggcgcgattc gcagccgtat ctgacagggg
    10381 agcggggcgg caacatggcg ttggcaacca aacacgaagc cgggcagggc catctccagc
    10441 acctggagca ggtgggcaag ggggcggcga ttgccaacga gatcttcggg ctggtcggcc
    10501 gctcgcagtt cttcgccgag ttctcgcgcg aggacatctc ggtcctggcc ggctacatgg
    10561 acgtctaccg ggcgcagccg ggcgagacca tcatccgcga ggacgatggc ggcgacttca
    10621 tgctgctcgt catcgagggc gccgtggata tcttcaagaa aggcatgcgc ggcgagcagc
    10681 agcacatgac caacgtcggt ccgggcatga cgctgggcga aatgtccatg atcgacggcg
    10741 agccgcgttt cgccacctgt atcgcgaccg aacccaccgt gttcgccgtg ctccaccgcg
    10801 aggacatggc gaagatcatc ctggatcact ccagcctggg ctccaagatc ctggtgaagc
    10861 tcgtctccat gctctccgcg cgcctgcgcc agaccagcgc gcggctgctg cagtacatgg
    10921 agcgcagcag cctcgtctga gccgcgcgtc cgctcggcaa gctgtgtcaa agcgattcgg
    10981 aaccacagat gaacacagat gcacacagat atc
//
LOCUS       DBNGSG010000406         6694 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_130617, whole genome shotgun sequence.
ACCESSION   DBNGSG010000406 DBNGSG010000000
VERSION     DBNGSG010000406.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6694)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6694)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6694
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_130617"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..1653
                     /gene="ubiB"
                     /locus_tag="AC158Q_10535"
     CDS             124..1653
                     /gene="ubiB"
                     /locus_tag="AC158Q_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HIR3340252.1"
                     /translation="MHLFRLLKILSVGVRYGLDEFLLGHERMAGLRALINSAFFWRTL
                     TESRAVRLRLALEELGPIFVKFGQVLSTRRDLLPSDIADELAKLQDRVPPFPAEEVMA
                     ILQRVYGKPAGEVFRDFSPLPVASASVAQVHLAALPDGTEVAVKVLRPGIATVISRDL
                     ALLHAGASLMEWLWADGRRLRPHEVVDEFSKHLEVELDLLREASNASQLRRNFTDSPL
                     LLVPAIHWDYCSSEVMVMERMHGTPVSQVAALRAQGVDIPRLARAGVEIFFTQVFRDG
                     FFHADMHPGNILVATEGEMGGRYVALDFGIMGTLTDIDKNYLAQNFLAFFRRDYRRVA
                     EAHIESGWAPKDTRVDDFESAIRAVCEPVFAKPLKEISFGRVLLRLFQTSRRFNIQVQ
                     PQLVLLQKTLLNVEGMGRDLDPDLDLWETAKPYLERWMAEQVGWRGLVERVRQEAPYW
                     STILPQLPRLLHQTLARQAQAGADEDLAALLREARRRNHWLAAIAALLALLAVIALAS
                     G"
     gene            1723..3174
                     /locus_tag="AC158Q_10540"
     CDS             1723..3174
                     /locus_tag="AC158Q_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high affinity choline transporter 1"
                     /protein_id="HIR3340253.1"
                     /translation="MLLTFVAAYLAVSIAIGLVAALKVHSAKDYITTGRRLPIWVVFA
                     AVFATWFGAETVLGISATFLREGLSGLVSDPFGASMCLILVGLFFARPLYRMNLLTIG
                     DYYRARYNRPVEVVTSLCIALSYLGWVAAQVTALGLVFNVISQGEISQTAGMIAGASV
                     VLAYTLFGGMWSVAFTTFVQMIIIVVGLFYIAWLVSGMAGGVTAVVAHAAAAGKFEFL
                     PRLEWRDVIAFVAAWVTLGLGSIPQQDVFQRVNASKNEGTAAWGSILGGSAYFLFAFV
                     PLFLAYSATLIDPGMVEKLIGGDSQLILPTLILNHLPLHAQVIFFGALLSVIMSTASG
                     TLLAPSVTISENVLRGLFREMSDRRFLWMTRAVVVCFTVLVTGYAVHTDATIHKMVEN
                     AYKITLSAAFTPLVFGLYWRRATTQGAAGAIVAGLVTWITLEFAAPDALFPPQFAALL
                     ASMVAMLAGSLAPQWYGTPRGGAAVGSAGGRSS"
     gene            3213..3719
                     /locus_tag="AC158Q_10545"
     CDS             3213..3719
                     /locus_tag="AC158Q_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340254.1"
                     /translation="MAAIFTLLTINAVAFLASGTLSEALDSVAWLTLLALFEVETGHT
                     VALSGRRATAAIHAVRLVAIAALIAALIGYVREKEWLDVLNVGLWVAVVALLELEVRR
                     PETVMRHRGAFSAAALALYAGLSALVVAWLWRGDWFFAYDAALWLIAFATLEMDVFGF
                     IGRDRSAP"
     gene            complement(3772..4728)
                     /locus_tag="AC158Q_10550"
     CDS             complement(3772..4728)
                     /locus_tag="AC158Q_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008924287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HIR3340255.1"
                     /translation="MQFYDIFNGDADGICALQQLRLEEPRASVLVTGVKRDVRLLDRV
                     NAAAGDELTVLDISLRANANGLARLLEAGARLRYYDHHAAGEVPRHANLAAFIDTAPD
                     TCTSLIVNRCLGGRQRAWAVVAAFGDNLPGPARRAAGGLDLDGAEIEQLRELGEFINY
                     NAYGETVDDLHYHPADLFETVSHYPDPRDFISGEPVFEVLRDALADDTERAAALVPQV
                     ADGGAALYVLPDAAWSRRIHGFFANQLALRHPSRAHALLVAGRGGYTVSVRAPAQNPR
                     GADVLCLKFETGGGRQAAAGINRLPEADYARFAAEFSKAFPG"
     gene            4937..5929
                     /locus_tag="AC158Q_10555"
     CDS             4937..5929
                     /locus_tag="AC158Q_10555"
                     /inference="COORDINATES: protein motif:HMM:NF016763.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIR3340256.1"
                     /translation="MLPFQGWRAPPEEIRQFLTMWPRFITLQDVAINVAAYVPLGLLA
                     SIGFGARHGPARGVVAATLAAAVLSLGMEALQMFLPARIASNVDLLANGLGALLGAIA
                     APLLAPNRTLGGALHAARRRFFLDGMAADFGMVVVLLWLVTQFHPTVQFFGTGNFRAT
                     LELPVYFTHTPGLAFSSEAAVAMLNLSGVGLMVSALMRGGTRPLLVAGTVVGAALAIK
                     VFTAAVLVQAPSPLAWLTPGVLAGLAAGCLLHYAATRLSSPAQLAISALCIVAATVAI
                     NLAPDNPYQSIPPRLLASGASHFLRISGIVRAVSELWPLLATGFLLYALSTRGR"
     gene            5992..6300
                     /locus_tag="AC158Q_10560"
     CDS             5992..6300
                     /locus_tag="AC158Q_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HIR3340257.1"
                     /translation="MTYYARHVFFCCNQRQEGEQCCNNSGSQKMRDYAKDRVKQLKLS
                     GAGKVRINQAGCLDRCKEGPVMVVYPEAVWYTYVDQRDIEEIIDEHLVNGRIVERLRI
                     "
     gene            6412..>6694
                     /locus_tag="AC158Q_10565"
     CDS             6412..>6694
                     /locus_tag="AC158Q_10565"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004633816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3340258.1"
                     /translation="MPAPEIRPMPAASLTRVMIEGPAGRIETDINDPGGARRGIALIA
                     HPNPVQGGTKDNKVVTTLAKTFFALGYVTARPNFRGVGSTEGRHDEGRGE"
BASE COUNT          979 a         2277 c         2179 g         1259 t
ORIGIN      
        1 tccgttcgaa cacgcggagt taaaccggcg attagggcac gggagccgcg gctcccgtat
       61 ccctgggcgt ccggatttaa cacgacttgg cgcgcttggc gtcttggcgg ttagaatcgt
      121 tgcatgcatc tgtttcgatt actgaaaata ctctccgtcg gcgtccgcta cggactcgat
      181 gaattcctgc tcggccacga gcggatggcc gggctgcgcg ccctcatcaa ctcggccttc
      241 ttctggcgga cgctcaccga atcgcgcgcg gtgcggctgc gcctggcgct ggaagagtta
      301 gggccgatct tcgtcaagtt cggccaggtg ctgtccacgc gccgtgattt gctgccgtcc
      361 gacatcgcgg acgagctggc gaagctccag gaccgggtgc cgccgttccc ggccgaggaa
      421 gtcatggcga tactgcagcg cgtctacggc aagcccgccg gggaggtgtt ccgggatttc
      481 agtcccttgc cggttgcgag cgcgtccgtc gcccaggtgc acctcgccgc cctgcccgat
      541 ggcaccgagg tcgcggtcaa ggtgctgcgt cccggcatcg cgacggtgat ctccagggac
      601 cttgcgctgc tccacgcggg cgcctccctg atggaatggc tgtgggccga cggcaggcgg
      661 ctgcgcccgc acgaggtggt ggacgaattc tccaagcacc tcgaggtcga gctcgatctg
      721 ctgcgcgaag cctccaatgc gagccagttg cgccgcaatt tcaccgattc gccgctgctg
      781 ctcgtcccgg ctatccactg ggactattgc tccagcgaag tgatggtgat ggagcgcatg
      841 catggcacgc cggtgtcgca ggtggcggcg ttgagggcgc agggcgtgga tatcccgagg
      901 ctcgcgcggg ccggcgtgga gatcttcttc acgcaggtat tccgcgacgg cttcttccac
      961 gccgacatgc accccggcaa catcctggtg gccaccgagg gggagatggg cggccgctac
     1021 gtcgcgctcg atttcggcat catgggcacg ctgaccgaca tcgacaagaa ctacctcgcc
     1081 cagaactttc tcgccttctt ccgccgcgat taccggcgcg tggcggaagc gcacatcgag
     1141 tcgggctggg cgccgaagga cacgcgggtg gatgatttcg agtccgcgat ccgcgccgtg
     1201 tgcgagccgg tcttcgcgaa accgctcaag gaaatctcgt tcgggcgcgt gctgctgcgc
     1261 ctgttccaga cctcgcgccg cttcaatatc caggtccagc cgcagctcgt tctgctgcag
     1321 aaaacgctgc tcaacgtcga gggtatgggc cgcgatctcg accccgacct cgatttgtgg
     1381 gagaccgcca agccttatct cgaacgctgg atggccgagc aggtcggctg gcgcgggctt
     1441 gtcgagcgcg tccggcagga agcgccgtac tggagcacga tcctgccgca gctgccgcgg
     1501 ctgctgcacc agacgctcgc gcgccaggcg caggccggcg cggacgagga cctggccgcc
     1561 ctgctgcggg aagcgcggcg ccgcaaccat tggctggccg cgatcgcggc cctgcttgcg
     1621 ctgctggccg tgatcgcact cgcgagcggc tgatgccggc gctcacgccg ttataatccg
     1681 gctttcgcga ttctccggca cgcgccgccg gtcaacgtat tcatgctgct cacgttcgtc
     1741 gccgcctacc tggccgtctc gatcgccatc ggcctggtcg ccgcgctgaa ggtccacagc
     1801 gcgaaggact acatcaccac cgggcgcagg ctgccgatct gggtcgtttt cgccgcggtg
     1861 ttcgccacct ggttcggcgc ggaaaccgtg ctcgggatct ccgccacctt tctgcgcgag
     1921 ggcctctccg ggctggtatc ggaccccttc ggcgcttcga tgtgcctgat cctggtggga
     1981 cttttcttcg cgcggccgct ctaccgcatg aacctactca ccatcggcga ctactaccgc
     2041 gcccgctaca accgcccggt ggaagtcgtc accagcctct gcatcgccct ctcctacctg
     2101 ggctgggtcg ccgcccaggt gacggccctg ggcctggtgt tcaacgtcat ttcgcagggg
     2161 gagatatcgc agaccgccgg catgatagcc ggcgcgtccg ttgtgctcgc ctataccctg
     2221 ttcggcggca tgtggtcggt cgcgttcacc actttcgtcc agatgatcat catcgtggtg
     2281 ggcctctttt acatcgcctg gctggtgagc gggatggcgg gcggcgtcac cgccgtagtg
     2341 gcgcacgcgg cagcggcggg aaaattcgag ttcctgccca ggctcgaatg gcgcgatgtc
     2401 atcgcgttcg tcgcggcatg ggtcacgctc gggctgggat cgatcccgca gcaggacgtg
     2461 ttccagcggg tcaatgcctc gaagaacgag ggcacggcgg cgtggggctc gatactcggg
     2521 ggatccgcct acttcctgtt cgctttcgtg ccgctgtttc tcgcctattc cgcgaccctg
     2581 atcgatcccg ggatggtcga aaagctgatc ggcggcgatt cccagctgat cctgcccacg
     2641 ttgatcctca atcacctgcc gctgcacgcg caggtcattt ttttcggggc gctgctctcg
     2701 gtcatcatga gcacggcgag cgggacgctg ctcgcgccct cggtgacgat ctcggagaac
     2761 gtgctgcgcg gcctgttccg ggaaatgtcc gaccgccgct tcctgtggat gacccgtgcc
     2821 gtggtggtgt gcttcaccgt gctcgtgacc ggctacgccg tgcataccga cgcgaccatc
     2881 cacaagatgg tcgagaatgc ctacaagatc acgctctcgg ccgcgttcac tccgctcgta
     2941 ttcggcctct actggcggcg ggccaccacc cagggcgcgg cgggcgcgat cgtggcgggt
     3001 ctcgtcacgt ggatcaccct ggaattcgcg gcgcccgacg ctctcttccc gccccagttc
     3061 gccgcgctgc tcgccagcat ggttgccatg ctggccggat cgctcgcccc gcagtggtat
     3121 ggaaccccgc gcggcggcgc agccgtcggg tcagccgggg gccgtagttc gtgattctcg
     3181 tccgggcaac gccggcgtgg cgggcctggc tcatggccgc gatcttcacg ctgctcacca
     3241 tcaacgccgt ggcgtttctg gcgtccggga cgctcagtga ggcactcgac tcggtggcct
     3301 ggctcaccct gctcgcgctt ttcgaagtgg agaccgggca caccgtcgct ctgagcggcc
     3361 ggcgcgcgac cgcggcgatc catgccgtgc gtctggtggc gattgccgca ctgatcgccg
     3421 cgctgatcgg ttatgtccgc gaaaaggaat ggctcgacgt ccttaacgtc ggactgtggg
     3481 tcgccgtggt ggcgctgctg gagcttgaag tacgccgccc cgagacggtg atgcggcacc
     3541 ggggagcatt ctcggcggcc gccctggcgt tgtatgcggg gctgtcggcc ctggtggtgg
     3601 cctggctttg gcgcggcgac tggttcttcg cctacgacgc ggccctgtgg ctcatcgcgt
     3661 ttgccacgct ggaaatggac gtgttcggtt tcatcgggag ggaccgcagc gcgccatgac
     3721 ggacccgatc cgggcctcgc gcgccgcggc agccggcacc gggttcagcc atcagccggg
     3781 aaatgccttg ctgaattccg cggcgaagcg cgcgtaatcc gcttccggca gccggttgat
     3841 ccctgccgcc gcctgcctgc cgccgccggt ttcgaacttg aggcacaaca cgtcggcgcc
     3901 gcgcgggttc tgtgccggcg cgcgcacgct caccgtgtag ccgccccgcc cggcgaccag
     3961 cagcgcatga gcgcgactcg gatgccggag cgcgagctgg ttggcgaaga atccgtggat
     4021 gcgccggctc caggccgcat ccggcagcac atagagcgcc gcgcccccgt ccgccacctg
     4081 cggcacgagc gccgcggcgc gctcggtatc gtcggcaagc gcatctctca gcacctcgaa
     4141 caccggctcg ccgctgatga aatcgcgcgg atcggggtag tggctcaccg tctcgaacag
     4201 gtcggcgggg tgatagtgca ggtcgtccac ggtctcgccg taggcgttgt aattgatgaa
     4261 ttcaccgagt tcgcgcagtt gctcgatttc cgcgccgtcg aggtcaagcc cccctgccgc
     4321 gcgccgcgcg ggcccgggaa gattgtcgcc aaaagccgcc accactgccc acgcgcgctg
     4381 gcggcccccg agacagcggt tcacgatgag gctggtgcag gtgtccggcg cggtgtcgat
     4441 gaacgccgcc aggttggcgt gccgcggcac ttcgcccgcc gcgtgatgat catagtaacg
     4501 caggcgcgcg cccgcctcga gcaatcgcgc gaggccattc gcgttggccc ggagagaaat
     4561 gtcgagcacc gtgagctcgt cgcccgccgc ggcgttgacg cggtcgagca gccgcacgtc
     4621 gcgcttgacc ccggttacca ggacgctcgc ccgcggctcc tcgagccgga gctgctgcag
     4681 cgcgcaaatg ccgtcggcgt cgccgttgaa gatgtcatag aattgcatcg gttacccgcg
     4741 cgcgcatcat ttgccaccgg ccaattcgaa agcgacgccc gcgggcgcgc ctcgaatcgc
     4801 cgcctgaaga cccatgaatc aagccccgcg acctgacaac tcgctccaat cgcacggcga
     4861 aggattttcg ccgcacagca tacgactggc gtgggtgctc accgtagctt atctagttgt
     4921 ggttgtgtat gccagcttgc tgccgtttca gggctggcgc gcgccgccgg aggaaatccg
     4981 gcagttcctc actatgtggc cgcgtttcat cacgctgcag gacgtcgcga tcaacgtcgc
     5041 ggcctacgtt ccgctcggcc tgctcgcctc gatcggcttc ggcgcgcgtc acggcccggc
     5101 gcggggggtt gtcgccgcca cgctggcagc ggcggtcctg agcctcggca tggaagcgct
     5161 gcagatgttc ctgcccgccc gcatcgcatc caacgtggac ctgcttgcca acggcctggg
     5221 tgcgttgctt ggcgcgatcg cagcgccgct gctagcgccc aaccgcaccc tcggcggcgc
     5281 gctgcatgcc gcccgccgcc ggttttttct cgacggcatg gcggccgatt tcgggatggt
     5341 ggtcgtcttg ctctggctcg tcactcaatt ccatcccacg gtgcagttct tcggcacggg
     5401 caacttccgc gccacgctcg aactgcccgt ttatttcacc cacacgcccg ggctcgcgtt
     5461 ctccagcgag gccgcggtgg ccatgctcaa tttgtcgggt gtggggctca tggtgtccgc
     5521 gctcatgcga ggtggcacgc gccccctgct cgtggccggt accgtggtcg gggcggcgct
     5581 cgccatcaag gtttttaccg ccgccgttct ggtccaggcg ccttcgccgc tcgcctggct
     5641 cacgcccggc gtgctcgccg ggcttgccgc cggctgcctg cttcattacg cggcgacacg
     5701 cctgtcgagc ccggctcagc tcgcgatctc ggcgttgtgc atcgtggctg ccacggtggc
     5761 gatcaatctc gcccccgaca acccctacca gagcattccg ccgcgcctgc tggcgagcgg
     5821 cgcgagccat tttctgcgca tttccggcat cgtgcgcgca gtctccgaat tgtggccttt
     5881 gctcgcgacc gggtttctgc tctacgccct gtccacccgc gggcgataag ctgccgggcc
     5941 aagcacgttc atcctatata attgcgcctc ttttcccacc gcatccgccc catgacctat
     6001 tacgcaagac acgttttttt ctgctgcaac cagcgccagg aaggcgagca gtgctgcaac
     6061 aacagcggct cgcagaagat gcgcgattat gccaaggacc gagtcaagca gctgaagctc
     6121 tccggcgcag gcaaggtgcg catcaaccag gcgggatgcc tcgaccgctg caaggaaggc
     6181 ccggtgatgg tggtctatcc cgaggcggtc tggtacacct atgtggacca gcgcgacatc
     6241 gaggagatca tcgacgagca cctcgtcaac ggccgcatcg tcgagcgcct gcgcatttag
     6301 atccgtttct cgttcttcgt ttctcgattc tcgtgcagat cgatcctgac gcttcgcgat
     6361 tgcgggattg atgaggaaag agcaacgagt aacgagtcac tctttcagtc catgcctgca
     6421 cccgaaatcc gtcccatgcc cgccgcgtcg ctgacgcggg tgatgatcga ggggccggcg
     6481 ggcagaatcg agaccgacat caacgatccc ggcggcgcgc ggcgcggcat cgcgctgatc
     6541 gcgcatccca acccggtgca gggcgggacc aaggacaaca aggtggtcac cacgctcgcc
     6601 aagacgttct tcgcgctggg ctacgtgacg gcgcggccca acttccgcgg cgtaggctcc
     6661 accgaaggcc ggcacgacga aggccgcggc gaga
//
LOCUS       DBNGSG010000407         8758 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_981168, whole genome shotgun sequence.
ACCESSION   DBNGSG010000407 DBNGSG010000000
VERSION     DBNGSG010000407.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 8758)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8758)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8758
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_981168"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="AC158Q_10570"
     CDS             <1..417
                     /locus_tag="AC158Q_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HIR3340259.1"
                     /translation="DNIEHDTIRAEGAYNDPRIHFAVNCAAISCPMLREEAYVAELLD
                     RQLEEQTVRFLSDRSRNRYNPETRALEVSRIFDSIPWYGGDFKRGWKGYKSLDQFFAQ
                     YAAVLAAGPEHRKLVREQKVDIRFLDYDWGLNDARQ"
     gene            495..1172
                     /locus_tag="AC158Q_10575"
     CDS             495..1172
                     /locus_tag="AC158Q_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HIR3340260.1"
                     /translation="MLSIIVPCLNEVDGIAAAMSALAPLRARGAEVIVVDGGSSDGTA
                     ECAAPYADAVIGAPRGRAAQMNAGAARARGEIMLFLHVDTLLPGSADALIIEGLKRTR
                     RGWGRFDVTIAGRHPLLRVVERFMNLRSRLSGIATGDQAIFVTRSLFTAVGGYPEIAL
                     MEDVALSKRLKRFGRPLCLRHRLTTSGRRWEKHGVLRTIVLMWLLRLAYWLGADPAKL
                     AMRYDRT"
     gene            1159..1815
                     /locus_tag="AC158Q_10580"
     CDS             1159..1815
                     /locus_tag="AC158Q_10580"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HIR3340261.1"
                     /translation="MTVPDVTAVIVFARAPRPGAVKTRLMPLLGAEGAAALHAQFVER
                     TLTTAQATSLHPIELHCAPDTNDLFFQFCAEHYGITVKPQVEGDLGGRMWSAFRQALT
                     AHARVLLVGSDCPALTPRHLRQAETALRDGADAVFVPCEDGGYGLIGLTRVDGMLFDG
                     IAWGGTDVMAQTRARLQTLGWTWRELETLWDVDRPEDYQRLVESGLLDTRRAAMMNAE
                     "
     gene            1889..2746
                     /locus_tag="AC158Q_10585"
     CDS             1889..2746
                     /locus_tag="AC158Q_10585"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000098 - sulfur amino acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HIR3340262.1"
                     /translation="MHSVRYTTLVSASELAAHLDDAGWVVCDCRHDLADTETGRRAYR
                     ESHIPGARFVHLDEDLSAPRTGRNGRHPLPDPDRFARRLAELGIGNDTQVAAYDASGG
                     YYAARLWWMLRWLGHGAAAVLDGGWNAWLNAPHSITKEIPEVKPARFVRGRSLVHSVT
                     AGEIANDPGTGQRRLLDARAPNRYRGENETLDPVAGHIPAAANRFFQLNLNTAGEFKS
                     PAVLRQEFTAALGTTPPSDVVHYCGSGVTACHNLLAMEIAGLPGSRLYPGSWSEWCSD
                     PSRPVATGG"
     gene            complement(2757..3719)
                     /locus_tag="AC158Q_10590"
     CDS             complement(2757..3719)
                     /locus_tag="AC158Q_10590"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3340263.1"
                     /translation="MTMMTRGALAVMALVLLGIAPAHAQYPTRSIRWIVPFAAGSGTD
                     TVARIVQPQLSAALGEQVVIENRPGAAGNLGAQVAAGTPADGYNLFMGISAHSISMTL
                     YRKPGFDLARDFMPVSLLAEGSFTLATHPSLPVKTVKELVAFAKKRPGEINVATAGAT
                     IRLAAKMLDIMAGTRMTEVTYKGTPQAIIAIVSGEASVSFPATSAAIPHARAGKLRAL
                     AVTTPRRSHMAPDLPTIAESGFPGYDVTTWYGLMFPTGTASEIVGRMHGAAAKALAHP
                     AVKERFAKTDLEPKISTPEEFGAYIHKEIAKWAKVIKTSGIAIE"
     gene            3860..4831
                     /locus_tag="AC158Q_10595"
     CDS             3860..4831
                     /locus_tag="AC158Q_10595"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012913.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HIR3340264.1"
                     /translation="MESASNLPLVAILATGGTIANTPSGRLHAGEVAEAIPALNKVAR
                     LEVEEFIRVASSAIGHEHWLPLARRVNELLSQPRVKGVVVTHGSNTVEETAYFLGLTV
                     KSDKPVVLTAAQRQFTTLSSDSPKNFLQAVRVAAGDEARGKGALVVTNDVIHAARDVS
                     KDISTRLETYSSRDIGALGFVDDDRVTFYRIPVKQHTLATPFDVLRIEELPRVDIVYT
                     YAGADGALIDAAVAHGARGIVIAGFPTGVATPVMDESIRRTVAGDIPVVMTNRGGKGR
                     VMDKKPGEARPLVWGDNLTPVKARVLLMLALTATRDRAALQRMFEVY"
     gene            complement(5261..5743)
                     /locus_tag="AC158Q_10600"
     CDS             complement(5261..5743)
                     /locus_tag="AC158Q_10600"
                     /inference="COORDINATES: protein motif:HMM:NF018755.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1353 domain-containing protein"
                     /protein_id="HIR3340265.1"
                     /translation="MSQFTKALVVTPLADGRTWVLMEPFSYDVGREGSGDTVNVPVKF
                     MTDFASVPRLLWIFLLQWGKYGNAAVIHDWGYWSKERSKQQTDLIFLEGMKVLGVPPF
                     KRTVMYLAVHLFGWWAWWSAGKRRARNEKKVAFRIPEKSTDEKADLAQHAAGKVESLT
                     "
     gene            complement(5831..6433)
                     /locus_tag="AC158Q_10605"
     CDS             complement(5831..6433)
                     /locus_tag="AC158Q_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003261878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HIR3340266.1"
                     /translation="MIGTFALVAGLAWASGLRLYLTLFVIGLLVHFQLAALPPAFDIL
                     GHPWVLAASGIMLLVEFLADKLPGFDSVWDSVHTFIRIPAGAILAGAAVGQADPVLVA
                     VAAILGGAIASGTHVAKSGTRALINTSPEPFSNWIASFGEEAVLLGGLWAMLKYPLAF
                     LIALALFILLLIWMIPRLVRAMRGVFRPRGGALQPPGGAA"
     gene            complement(6467..6754)
                     /locus_tag="AC158Q_10610"
     CDS             complement(6467..6754)
                     /locus_tag="AC158Q_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340267.1"
                     /translation="MNTSDEITEWLAKNGRKALTLDAARLLAAESPDHWYVASHGNKG
                     FLVGKMTLQKFSQRHILVDETGQAMHFATVEAALLFLRKDLRIPNPHVFNY"
     gene            complement(6823..7899)
                     /locus_tag="AC158Q_10615"
     CDS             complement(6823..7899)
                     /locus_tag="AC158Q_10615"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HIR3340268.1"
                     /translation="MNSPLDVHQSRILVVDDESGIRRLLGMELRAAGYGDVTLCSDSR
                     KVAELYREHRYELILLDLHMPNLDGYQVMSALKEIETDGYLPVLVITAEPKEKMKALR
                     AGAKDFVTKPFERAELLTRVKNLLEVRLLYRRLRDYSGDLEDKVRERTARLTGSYHET
                     LRTMTRAAEYRDDTTGAHVRRISFYSRELAERIGMDGGFCERIFHASPMHDIGKIGIP
                     DSILLKKGPLTNRQWEVMRMHTMIGARMLMDGESPYLQMGAQIAAAHHERWDGSGYPN
                     GLKGEEIPLAARVMAICDVYDALRAARPYKPALEHDSAVNIIVKGDRRTKPRHFDPAV
                     LDAFRRSAGAYRDIYAANADRPTG"
     gene            complement(8040..>8758)
                     /locus_tag="AC158Q_10620"
     CDS             complement(8040..>8758)
                     /locus_tag="AC158Q_10620"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3340269.1"
                     /translation="PDGYTLLMANSTISSPGLFRNLPFDVRKDLDPVALIAIGPSVLV
                     VHPAVPAKNVKDLIALARAQPKKLVYGSGGVGNVTHLEMEIMNAMVGVQMTHVPYKGS
                     APSLVGLVSGEVQALFSSIPAALGQIRAGRIRALGVSITKRSSVLPDVPTIHESGVPG
                     FDAASWYLVFAPAGTPKNVVGLLGKEIVRTMASPNIRGRFANDGFEPAGTGPAESARF
                     LRAELVKWDKAVKMAGIQPE"
BASE COUNT         1484 a         2939 c         2784 g         1551 t
ORIGIN      
        1 gacaacatcg agcacgacac aattcgcgca gaaggtgcgt acaacgaccc gcgcattcat
       61 ttcgcggtca attgtgccgc gatctcctgc ccgatgctgc gcgaggaggc gtacgtcgcg
      121 gaactgctag accggcaact cgaggagcag acggtccggt tcctttccga ccgctcgcgc
      181 aaccgctata acccggaaac ccgggcgctc gaggtctcca ggattttcga ttccattccc
      241 tggtatggag gggacttcaa gagaggctgg aagggctaca aatcgctcga tcaattcttc
      301 gcccagtacg ccgccgtgct ggccgccggg cccgagcacc ggaagctcgt gcgcgagcaa
      361 aaggtcgata tccgttttct ggactatgat tggggactca acgacgcaag acagtaatag
      421 acaggattaa caggattaac aagattcaat caaaatacag aatctggttt tgacgggttt
      481 tgacgagtct caggatgctc tccatcatcg ttccctgcct caacgaagtc gacggcatcg
      541 ccgccgcgat gtccgcgctt gcgccgttac gggcgcgcgg cgcggaagtg atcgtggtgg
      601 acggcggcag cagtgatggc acggcggaat gcgctgcgcc gtacgcggat gccgtgatcg
      661 gcgccccgcg cggacgcgcc gcccagatga acgccggggc cgcccgcgcc cgcggtgaga
      721 tcatgctgtt cctgcacgtg gataccctgc tgccgggctc ggccgatgcg ctgatcatcg
      781 aaggactgaa gcgcacgcgc cgcggctggg ggcgcttcga cgtcaccatc gccggccgcc
      841 acccgctgct gcgcgtggtg gaacggttca tgaacctgcg ctcgcggctg agcggaatcg
      901 ccaccggcga ccaggcaatc ttcgtgactc gctccttgtt caccgccgtg ggcggctatc
      961 ccgagatcgc actgatggag gatgtcgcgc tgtccaagcg tctcaagcgc ttcgggcggc
     1021 cgctgtgcct caggcaccgc ctcaccacct cgggccgccg ctgggaaaag cacggcgtgc
     1081 tgcgcaccat cgtgctgatg tggctgctgc ggctcgccta ctggctcggg gccgacccgg
     1141 ccaagctcgc gatgcgttat gaccgtacct gacgttaccg ccgtcattgt tttcgccaga
     1201 gccccgcggc ccggcgcagt caagacgagg ctgatgccgc tgctcggggc ggaaggggct
     1261 gcagcgctcc acgctcagtt cgtcgaacgc acgctcacga cagcgcaggc gacctcgctg
     1321 cacccgatcg agcttcactg cgctccggac accaacgacc tctttttcca gttttgcgcg
     1381 gaacactacg gcatcaccgt caagccgcag gtcgagggcg atctcggcgg gcgtatgtgg
     1441 tcagctttca gacaggcact caccgcccac gcgcgcgtcc tgcttgtcgg ctccgactgc
     1501 cctgccctca cgccgcgcca cctgcgccag gccgagacgg cattgcgcga cggcgccgac
     1561 gcggtgttcg tgccgtgcga ggacggcggc tacgggctga tcggcctcac gcgcgtggat
     1621 gggatgcttt tcgacggcat tgcctggggc ggcacggacg tgatggcgca aacccgcgcg
     1681 cggctgcaga cgctcggctg gacctggcgc gagctcgaga ccctctggga cgttgaccgg
     1741 ccggaagact accagcgcct cgtcgagtca ggtttgctcg atacgcgacg agccgcaatg
     1801 atgaatgcgg aatgacgaat gcggtattga aaaccctgct gcaagccacc agcctgttcc
     1861 gcccgttcat cattcttcac tcataagtct gcattccgtg cgttacacca ccctcgtatc
     1921 cgccagcgaa ctcgccgcac atctcgacga tgccggctgg gtcgtctgcg actgccgcca
     1981 cgatcttgcc gacaccgaaa cagggcgccg cgcctaccga gaatcccata tccccggcgc
     2041 gcgcttcgtg cacctcgacg aggacctgtc ggcccccagg acgggccgta acggccgcca
     2101 ccccctgccc gacccggacc gcttcgcgcg gcgccttgca gagctcggca tcggcaacga
     2161 tacgcaggtg gccgcctacg atgccagcgg cggctattac gccgcgcgcc tgtggtggat
     2221 gctgcgctgg ctggggcacg gcgccgcggc ggtgctcgac ggcggatgga atgcgtggct
     2281 gaacgccccg cattccataa cgaaagagat tcccgaggta aagccggcgc ggttcgtgcg
     2341 tggtcgctcg cttgtccaca gcgtgactgc gggggagatc gcgaacgatc ccggaacggg
     2401 acagcgccgg cttcttgatg cgcgcgcacc caaccgctac cgcggcgaga acgagacgct
     2461 cgacccggtg gccggccata ttcccgccgc agccaatcgt ttcttccagc tcaatctcaa
     2521 tacggccggc gaattcaagt caccggccgt attgagacag gaattcacgg cagcgctggg
     2581 caccacgcct ccatccgacg tcgtgcatta ctgcggcagc ggagtaacag cctgccacaa
     2641 tctcctcgcc atggagatcg ccggcctgcc cggctcccgc ctctatcccg gctcgtggag
     2701 tgagtggtgt tcggatccct cgcggcccgt cgccaccggc ggctgaataa aaacggctat
     2761 tcgatcgcga tgccggacgt cttgatcacc ttcgcccact tcgcgatttc cttgtgaatg
     2821 taggcgccga actcctccgg ggtggagatc ttcggctcga gatcggtctt ggcgaaccgc
     2881 tccttcacgg caggatgcgc gagcgctttg gccgccgccc cgtgcatccg tccgacgatc
     2941 tcgctcgccg tgccggtcgg aaacatgagg ccataccacg tggtcacgtc gtaaccggga
     3001 aagccggact cggcgatcgt cggaaggtcg ggggccatgt gcgagcgccg gggcgtggtg
     3061 acggcgagcg cgcgcagctt cccggcccgc gcgtgcggga tcgcggcgct ggttgcggga
     3121 aagctgaccg acgcttcgcc gctgacgatc gcgataatgg cctggggcgt gcccttgtag
     3181 gtgacctccg tcatccgggt gcccgccatg atgtcgagca tctttgccgc aagccgtatg
     3241 gtcgcgcccg ccgtggcgac gttgatctcg cctggccgct tcttcgcaaa ggcgaccagc
     3301 tccttcaccg tcttcaccgg caacgaggga tgcgtcgcga gcgtgaacga gccctcggcg
     3361 agcaacgaca ccggcatgaa atcccgggcg aggtcgaagc cgggtttgcg gtacagcgtc
     3421 atgctgatcg aatgggcgct gatgcccatg aaaaggttgt agccgtcggc cggagtgccg
     3481 gccgccacct gcgcgccgag attgccggcg gcgccggggc ggttctcgat gacgacctgc
     3541 tccccgagcg ccgcggagag ctgcggctga acgatgcggg cgacggtatc cgttccgctg
     3601 cctgcagcga acggcacgat ccagcggatc gatcgggtcg ggtactgggc atgggccggc
     3661 gcaatgccca ggagaacgag cgccatcacg gcaagggcgc cgcgcgtcat cattgtcatt
     3721 ttttcctcca tcgagtggtc ctgcgatttc cctctccgat cccgcacgga gcaggacgca
     3781 ttgtaaagca cgcccctcat attgcggcca ggaccgacgg cggttatcct ctggcgcgtc
     3841 attcaggatg agagatagcg tggaatcggc gagcaacctg cctcttgtgg cgatcctggc
     3901 gacgggcggg accatcgcca acacgccgtc gggccgcctg catgcgggcg aggtcgcgga
     3961 ggccataccg gcgttgaata aggtcgcgcg gctggaggtg gaggagttca tccgcgtagc
     4021 cagcagcgcc atcggccacg agcactggct gccgctcgcg cggcgcgtca acgagcttct
     4081 ctcgcaaccg cgggtaaagg gcgtcgtcgt cactcacggc tcgaacacgg tggaggagac
     4141 cgcctacttc ctcggcctca cggtgaagag cgacaagccg gtggtgctca ccgccgctca
     4201 aaggcagttc accaccctca gctccgacag ccccaagaac ttcctgcagg cggttcgcgt
     4261 ggctgccggc gacgaggccc gcggcaaggg cgcgctggtc gtgacgaacg acgtcatcca
     4321 tgcggcgcgc gatgtttcaa aggacatctc cacccggctg gaaacctaca gcagccgcga
     4381 catcggcgcg ctcggcttcg tcgacgacga tcgggtgacg ttctaccgca ttcccgtgaa
     4441 gcagcacacc ctggccacgc ccttcgatgt cctgcgcatc gaggagctgc cgcgggtaga
     4501 catcgtctac acctacgcgg gcgccgacgg cgccctgatc gacgcggccg tcgcgcacgg
     4561 cgcccggggc atcgtgattg ccggcttccc gaccggcgtt gcgacgccgg tcatggacga
     4621 gtcgatccgg cggacggtgg ccggagacat tcccgtcgtc atgacgaatc gcggcgggaa
     4681 ggggcgggtc atggacaaga agcccgggga agcgcgcccg ctcgtctggg gcgacaacct
     4741 cacgcccgtc aaggcgaggg tactcctcat gctcgccctg accgcaaccc gcgatcgcgc
     4801 cgcgctgcag cgcatgttcg aggtctatta agagtcccta acatgatgaa acgcgcgctg
     4861 cgctgtcacg attttggctc agggcgcgaa gcgagccgca gccaatatgt cgatattagc
     4921 aaggcgagcg acaaagccat gggccaaaac tcgtgccagc ccccatgttt cccttgaata
     4981 ttccgatgcc tgcgccgggg ttgcggccaa aatcggcgac gcgccgtaac gtggagcagg
     5041 ggccccggtt tccggggatg cgaccgtgca ggcgtgtgtg agtcccgcgc atgacggcgc
     5101 tctaatgtcc cgctccgggg tcacatccag gcacaaaccg cctggaacct gttccgccct
     5161 gcgcgggaca ctttgtcggc cggcttggtc ctatcgacat aggccctgcg ccgtccttcg
     5221 cgtcatcacc cattttggct cgcaacgcac gcgtgtttca ttatgttagg gactctactt
     5281 tgcccgccgc atgctgcgcg agatcggcct tctcgtccgt cgatttctcc gggatgcgga
     5341 aagcgacctt cttttcattg cgcgccctgc gcttgccggc gctccaccac gcccaccagc
     5401 cgaacaggtg cacggcgagg tacattacgg tgcgcttgaa cggcggcacg ccgagaacct
     5461 tcatgccctc gaggaagatc aggtcggtct gctgtttcga gcgttccttg gaccagtagc
     5521 cccagtcgtg gatcacggcg gcgttgccgt acttgcccca ttgcaggagg aagatccaca
     5581 acagccgcgg cacgctcgcg aaatcagtca tgaacttcac cggcacgttg accgtgtcgc
     5641 cgctgccttc ccgccccacg tcgtagctga acggctccat cagcacccat gtccgcccat
     5701 cggcgagcgg cgtgactacg agggccttgg tgaactggct catttgcgcc tccccttgca
     5761 ggacaaacga tgagagcaat ccgggaacga ggtgaaaccc cttccgcctt tcacctttcg
     5821 cctttcgact tcaagcagcg ccgccgggcg gttgcagcgc cccgccgcgg gggcgaaata
     5881 ccccgcgcat ggcgcgaacc agcctgggta tcatccagat aagcagcagg atgaagagcg
     5941 ccagcgcgat caggaacgcc agcgggtact tgagcatcgc ccacaagccg ccgagcagca
     6001 ccgcctcctc gccgaacgaa gcaatccagt tgctgaacgg ctcgggcgag gtgttgatca
     6061 gcgcgcgcgt cccggacttg gcgacgtggg tgcccgaggc aatcgcgccg cccagtatcg
     6121 cggccaccgc caccagcacc gggtcggcct ggcccaccgc ggcgcccgcc aggatcgcgc
     6181 cggcgggaat gcgaatgaag gtgtgcacgc tgtcccagac gctgtcgaac cccggcagct
     6241 tgtccgcgag aaactccacc aggagcatga tcccggacgc agccagcacc caagggtggc
     6301 cgagtatgtc gaaggcggga ggcagcgcgg cgagctggaa gtgcacgagc aggccgatca
     6361 cgaacagcgt gagatagagg cgcaatccgc ttgcccacgc cagcccggcg acgagggcga
     6421 aggtgccgat cacggtctgg atagcttcca tgccgaaggc gccggttcag taattgaata
     6481 cgtgcgggtt cggaatcctc agatccttgc gcaggaacag cagcgcggcc tcgaccgtgg
     6541 caaagtgcat cgcctggccg gtttcatcca cgaggatgtg ccgctgcgag aatttctgca
     6601 gggtcatttt cccgaccagg aaacccttgt tgccgtggga ggccacgtac cagtgatccg
     6661 gcgattcggc ggcgagcagc cgcgcggcgt cgagcgtcag cgccttgcgc ccgttcttcg
     6721 ccagccactc ggtgatctcg tcagaagtgt tcattcgggt tcaggcggcg aaaccgtgtc
     6781 cgatcccttt cgctgctttt tcaacctgga aacctgcttt cttcagcctg tcggccggtc
     6841 ggcattggcc gcgtagatgt cacgatacgc tcccgcgctg cgccggaacg catcgagcac
     6901 ggcgggatcg aaatgcctcg gcttggtccg gcgatcgccc ttgacgatga tattcacggc
     6961 gctgtcgtgc tcgagcgcgg gcttgtacgg ccgcgcagcg cgcagcgcgt cgtacacgtc
     7021 gcatatcgcc atgacccgtg ccgcaagcgg aatctcctcg cccttcagcc cgttcgggta
     7081 gccgctcccg tcccagcgct cgtgatgcgc cgccgcgatc tgggcgccca tctgcaggta
     7141 cggcgattcg ccgtccatca gcatccgcgc cccgatcatg gtgtgcatcc gcatgacctc
     7201 ccattgccgg ttggtcagcg gccctttttt cagcaggatg gagtcgggaa tgccgatttt
     7261 tccgatatcg tgcatcgggc tcgcgtggaa gatccgctcg cagaatccgc catccatgcc
     7321 gatgcgctcg gcgagttccc gcgagtagaa gctgatgcgc cgcacgtgcg cgccggtagt
     7381 gtcgtcgcgg tattccgccg cgcgcgtcat cgtccgcagc gtctcgtgat agctgccggt
     7441 gaggcgggcc gtgcgctccc ggaccttgtc ttcgagatcc ccgctgtagt cgcgcagccg
     7501 gcgatacagc agccggactt ccagcaggtt tttcacgcgg gtgagcagct cggcgcgctc
     7561 gaacggcttg gtgacgaagt ccttggcgcc tgcccgcagg gctttcatct tctccttcgg
     7621 ctcggcggtg atcaccagca ccggcaggta gccgtcggtt tcgatttcct tgagcgccga
     7681 catcacctgg taaccgtcca ggttgggcat gtgcaggtcg agcaggatca gctcgtagcg
     7741 gtgctcccgg tacagctccg cgacctttct ggaatcgctg cagagcgtga cgtcgccgta
     7801 tccggcggcg cgcagctcca tgccgagcag ccgcctgatg ccggactcgt catcgaccac
     7861 cagaattcgc gattgatgca catcgagtgg tgaattcatc tcccggccca tcctggttcc
     7921 aatggcactt taaacggagt ctgccctttt ttatcgttcc gcgcgatcaa ttcatcggaa
     7981 cggggccctg ttcgcggtgc ccggcccccg tcgggcccgc cgcaagcccc atcaacgatc
     8041 tattcgggct ggatgcccgc catcttcacc gccttgtccc acttgacgag ttccgcgcgc
     8101 aggaacctcg cggactccgc cgggcccgtg ccggccggct cgaagccgtc gttcgcgaac
     8161 cgcccgcgga tattgggcga ggccatggtc ctgacgatct ccttgcccag caggccgacc
     8221 acgttcttcg gcgtgccggc gggcgcgaag accaggtacc aggacgccgc atcgaagccg
     8281 ggcacgcccg actcgtggat ggtcggcacg tcgggcagca cgctgctgcg cttggtgatc
     8341 gacacgccca gcgcgcggat tctccccgcg cggatctgcc ccagcgcggc cgggatggaa
     8401 gagaacagcg cctgcacttc tccgctcacc aggccgacga gcgagggcgc gctgcccttg
     8461 tacggcacgt gcgtcatctg cacgcccacc atcgcgttca tgatttccat ttccaggtgg
     8521 gtgacgttgc cgacgccgcc ggagccgtag accagcttct tcggctgcgc tcgcgccagc
     8581 gcgatcaggt ccttcacgtt tttcgccggg acggctggat gcacgaccag cacggacggc
     8641 ccgatcgcga tcagcgcgac cgggtcgaga tccttgcgca cgtcgaacgg caggttcctg
     8701 aacaggcccg ggctcgagat ggtggaattg gccatcagca gcgtgtagcc gtccggcg
//
LOCUS       DBNGSG010000408         3576 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1490045, whole genome shotgun sequence.
ACCESSION   DBNGSG010000408 DBNGSG010000000
VERSION     DBNGSG010000408.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1490045"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..896
                     /gene="map"
                     /locus_tag="AC158Q_10625"
     CDS             84..896
                     /gene="map"
                     /locus_tag="AC158Q_10625"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HIR3340270.1"
                     /translation="MAVSIKSPQEIEKMKVAGQLAAEVLDFIAPHVKAGMTTGALDRL
                     CHDYISREQKATPAPLNYAPPGYQPYPKSICTSVNHVVCHGVPGDKKLRTGDIINIDI
                     TVIKDGYHGDTSRMFYVGTPSIQARRLCEVTYECLWRGIDQVRPGSRLGDIGHAIQVH
                     AESHGYSVVREFCGHGIGSKFHEEPQVLHYGRPGIGLRLETGMTFTIEPMINAGKPDI
                     RQLADGWTVVTKDHSLSAQWEHTVLVTETGHQVLTLSAGSPASPAQVSRTHV"
     gene            889..3477
                     /locus_tag="AC158Q_10630"
     CDS             889..3477
                     /locus_tag="AC158Q_10630"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HIR3340271.1"
                     /translation="MSELPAVTAALPVQTRTGHWKRRLAALREQLRQEFSERESPREH
                     LRRQAAVIDRLLKDLWAGHAMPRNVALAAVGGYGRGQLFPCSDVDLLILLSSPAETPL
                     ARKIEELIGTLWDIGIEVGHSVRTIDECAALAAADVTVQTTLLEARLLAGKRDLFARF
                     ARRMHDELDPAAFMQAKLLEQQQRHMRYAETNLEPNIKESAGGLRDLQTILWISRAAG
                     IGKTWSELARRGVITRAEAREIAQHELLLQSLRVRLHYCAGRREDRLLFDYQSALARE
                     FDLHDRPHRLASEQLMQRYYRTAKAVFQLNTIVLQNLDARITPPRDKEYRPINERFGV
                     RDELLVPRDERLFQRQPAAILESFLLLQQHPELKGMSAPALRALWRARRLINPQFRKN
                     PRNRDQFLRLLRSPERVARTLKRMNQYDVLGRYLPVFGRIVGQMQHDLYHVYTVDEHI
                     LKVLRNVRRFAVPELAHEFPLCSRLMSNFERPEVLYLAALFHDIAKGRGGDHSRLGAT
                     DARRFCREHGLAREDVELVAWLVESHLVMSATAQKQDIADPDVVRAFAERVGDERHLL
                     ALYLLTVADIRGTSPRVWNAWKGKLLEDLFWATRRMLSGAQLTLASSLQVRQEEARAK
                     LRLYAVPAEAEKKLWAQLDDSYFLRHEPQEIAWHTRMLYYRVQSQEPVVKARLSPAGE
                     GLQVMIYVPDQKELFARICSFFERISYDIFEAKVYTTSHGYALDSFQVQDPDNRRPQY
                     RDVIGLIEHDLAERLLQKTPLPALSKPRLSRQLRHFPISPEVSIQPDEKGTSWVLSVI
                     AGDRPGLLSRIARVLTAYDVNLSTAKINTLGSRAEDVFIIAGEALKDAKTVVRLESAL
                     LEQLQT"
BASE COUNT          689 a         1166 c         1118 g          603 t
ORIGIN      
        1 ggcgcgccga tctggactaa aatccgctgt ccttgaaaaa ccaggtactt actctctcgc
       61 cgcaacggcg cgggtcaggc tcaatggccg tcagcatcaa gtccccgcag gaaatcgaga
      121 aaatgaaggt ggcgggccaa ctcgccgccg aagttctcga cttcatcgct ccccacgtca
      181 aggccggcat gacgaccggc gcgctcgacc ggctttgcca cgactacatt tccagggagc
      241 agaaggcgac gcccgcgccg ctgaattacg ccccacccgg ctaccagccg taccccaagt
      301 cgatctgcac ctcggtcaat cacgtggtct gtcacggcgt ccccggcgac aagaagctgc
      361 ggaccgggga catcatcaac atcgatatca cggtcatcaa ggacggctat catggtgaca
      421 ccagccgcat gttctacgtc ggcacccctt cgattcaggc gcggcggcta tgcgaagtaa
      481 cctatgaatg cctgtggcgt ggcatcgacc aggtacggcc aggctcccgc ctgggcgata
      541 tcggccatgc catacaagtt catgcggaga gccacgggta cagcgtggtg cgcgagttct
      601 gcggccacgg tatcggcagc aaatttcacg aggagccgca ggtgctgcac tatggcagac
      661 cgggcatcgg actgcggctc gagacgggca tgacattcac cattgagccc atgatcaacg
      721 ccggcaaacc ggatatccgc cagctcgccg atggctggac cgtcgtgacc aaggaccaca
      781 gcctgtcggc gcagtgggag cacaccgtgc tggtgacgga aaccggacac caggtgctca
      841 cactctcggc gggctctccc gccagccctg cccaggtttc ccggacgcat gtctgaactg
      901 ccggccgtta ctgctgccct tccagtacag acacgcaccg gccactggaa gcggcggctg
      961 gccgcactgc gcgaacagct gcgccaagag ttctccgaac gcgaatcgcc gagagaacat
     1021 ctgcgccggc aggctgcggt aatagaccgc ctgctgaagg atttgtgggc gggtcatgcc
     1081 atgccgcgta acgtggcgct ggccgcagtc ggcggctacg ggcgcggcca gctatttcca
     1141 tgctccgatg tcgacctgtt gatcctgctg tcttcgccgg cggaaacccc gctcgcgcgc
     1201 aagatcgagg aattgatcgg gaccctttgg gacatcggca tagaggttgg gcatagcgtg
     1261 cgaaccatcg acgaatgcgc cgcgctggcc gcggcggacg tcacggtcca gaccacgctg
     1321 ctggaagccc gcctgctcgc cggcaagcgc gacctgttcg cgcgctttgc caggcgcatg
     1381 cacgatgaat tggaccctgc cgctttcatg caggcgaagc tgctggaaca acagcaacgc
     1441 cacatgcgct atgccgaaac caatctcgag cccaacatca aggaaagtgc cggcggcctg
     1501 cgcgaccttc aaaccatact ctggatttcc cgagcggccg gtatcggtaa gacatggtcg
     1561 gagctcgcga ggcgcggtgt gatcaccagg gccgaggccc gcgagatcgc ccagcacgag
     1621 ttgctgctgc agtcgctgcg cgtccgcctg cattactgcg cgggcaggcg cgaagaccgg
     1681 ctgctgttcg actaccagtc cgcgctcgcc cgtgagttcg acctgcacga tcggccgcac
     1741 cggctggcga gcgagcaact gatgcagcgt tactaccgga cggcgaaggc ggtttttcag
     1801 ctgaacacca tcgtcctgca gaacctcgac gcgcgcatca cccctccgcg cgacaaggaa
     1861 taccggccga tcaacgagcg cttcggcgtg cgggacgagt tgctggtgcc gcgcgacgag
     1921 cgactgttcc agcgtcagcc ggcggcaatt ctggaaagct ttctgctgct gcagcagcat
     1981 ccggagctca agggcatgag cgcccccgcc ctgcgtgcgc tgtggcgggc gcggcgcctg
     2041 atcaatccgc aattccgcaa gaatccccgc aaccgggacc agttcctgcg gctgctgcgc
     2101 agcccggaac gggtggcgag aacattgaag cgcatgaacc agtacgacgt gctgggccgt
     2161 tacctgccgg tcttcggacg catcgtcggg cagatgcagc acgatctcta tcacgtctac
     2221 accgtggatg agcacatcct caaggtgctg cgcaacgtgc ggcgcttcgc cgtgccggag
     2281 ctcgcgcacg aattcccgct gtgcagccgg ctgatgagca atttcgagcg gccggaggtg
     2341 ctctatctgg cggcgctgtt ccacgacatc gccaaggggc gtggaggaga tcattcgcgg
     2401 ctcggagcaa cggacgcgcg ccggttttgc cgcgagcatg gattagcgcg ggaggatgtc
     2461 gagctcgtcg cctggctggt cgagagccac ctggtcatgt cggcgacggc gcagaagcag
     2521 gacatcgccg acccggacgt cgtgcgggcc ttcgcggagc gcgttggcga cgagcgccat
     2581 ctcttggcgc tctatctgct gacggtcgcg gatatccgcg gcaccagccc ccgcgtctgg
     2641 aacgcatgga agggcaagct tctggaggat ttgttctggg cgacacgacg catgctctcg
     2701 ggcgcccagc tcaccctcgc aagcagcctg caggtgcggc aggaagaagc ccgcgccaag
     2761 ctgcggctct acgcggtgcc ggcagaagcg gagaagaaac tgtgggcgca gcttgacgat
     2821 tcctacttcc tgcggcacga gccccaggag attgcctggc acacccggat gctctactac
     2881 cgcgtgcaat cgcaggagcc cgtggtcaaa gcgcgcctgt caccggcggg cgaaggcctc
     2941 caggtgatga tttatgtccc cgaccagaaa gagctgttcg cgcgcatctg cagtttcttc
     3001 gagcgcatca gctacgacat cttcgaagcc aaggtgtaca caacgagcca cggctatgcg
     3061 ctcgacagtt ttcaggtgca ggatccggac aatcgccgtc cgcagtaccg cgacgtcatc
     3121 gggttgatcg agcacgatct ggccgagcgc ctgctgcaaa aaacaccact gcctgcactc
     3181 tcgaagccgc gactgtcccg gcaattgagg cactttccga tctctcccga ggtcagcatc
     3241 cagccggacg agaaaggcac ctcctgggtg ctctcggtga tcgccggcga ccgccccggc
     3301 ctgctgtcgc gcatcgcgcg cgtgctgacc gcctacgacg tcaacctgag cacggccaag
     3361 atcaacacgc tcggctcgcg cgccgaggac gtgttcatca tcgcgggaga agcgctcaag
     3421 gacgccaaga ccgtggtgcg gctggagagc gccctcctcg agcagctgca aacctgatat
     3481 ggcgcgatgg gtgatgagtg acgagtgccg ggaacaaaac caggctcttg tcccatcact
     3541 cgtcctgcag gtcctgcccg ggaaataatt cctcgt
//
LOCUS       DBNGSG010000409         4300 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_982659, whole genome shotgun sequence.
ACCESSION   DBNGSG010000409 DBNGSG010000000
VERSION     DBNGSG010000409.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4300)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_982659"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /gene="rpoE"
                     /locus_tag="AC158Q_10635"
     CDS             <1..473
                     /gene="rpoE"
                     /locus_tag="AC158Q_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HIR3340272.1"
                     /translation="STEVEDVTQEAFIKAYRALPTFRGDSAFYTWLYRIGINTAKNYL
                     VAMGRRAPTTTEIDSEEAEGFEDGDQLRDLNTPEDELASRQVAETVNQTLGELPEELR
                     TAITLREIEGLSYEDIANIMNCPIGTVRSRIFRAREAIATKLRPLLGTQRDRRW"
     gene            475..993
                     /locus_tag="AC158Q_10640"
     CDS             475..993
                     /locus_tag="AC158Q_10640"
                     /inference="COORDINATES: protein motif:HMM:NF015810.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HIR3340273.1"
                     /translation="MDKISALMDGELDEHQTRQLLVRLKQDEELLQSWHTFHLIGDAL
                     RGEHPLSHDFDRRLTARLAGEPTVIGRRRNVTKRVVTYALSAAASLSAIALVGWVALS
                     DNPLGPQPELAKAPKPPAVAAPSAQLASVPSEGTMNEYLIAHQEFSPSTAIQGLAPYI
                     RSVSSTQPAQGR"
     gene            1086..1985
                     /locus_tag="AC158Q_10645"
     CDS             1086..1985
                     /locus_tag="AC158Q_10645"
                     /inference="COORDINATES: protein motif:HMM:NF015825.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HIR3340274.1"
                     /translation="MNWLKKMASASRELNYSGTFIYRHGYRTETSRVVHYVNAAGGEF
                     ERMETLDGPIREVIRTNDQVVCYLPKAKTVLIEKRGKRQFPAMLPDRLTGVSENYTVH
                     VGEMDRVAGHDCQVVVLEPKDRLRYGHQYCADAGSGLPLRARTFNEKKEPLESFVFTE
                     LVIGGSFNRDKVRSRYAAKSRDWKVDHSGYPINEVATDTGWVLTRQPAGFRKLTELKR
                     SIAGRAFAVSHIVYSDGLAAISVFIEPLPKVRIAQQLSHQGAVNIYIRPIADHMVTVL
                     GEAPAATVIQIANSLELKGAALR"
     gene            2046..3446
                     /locus_tag="AC158Q_10650"
     CDS             2046..3446
                     /locus_tag="AC158Q_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HIR3340275.1"
                     /translation="MFKRQLLRILFALCVVLPVASAAQLPDFTELVEKQGAAVVNIST
                     TQSVRNPLLPQVPNLQEDDPFYEFFRRFIPQPGPREFQSQSLGSGFIISQDGYILTNA
                     HVVETAEEITVKLTDKREFKAKVIGSDRRTDIALIKIESASLPAVRFGDPNRLKVGEW
                     VLAIGSPFGFENTVTAGIVSAKGRSLPQENYVPFIQTDVAVNPGNSGGPLFNLRGEVV
                     GINSQIYSRTGGFMGLSFAIPIDVANDIAQQLRTTGKVTRGRIGVVIQPVTKELADGF
                     GLPRAQGALVNSVEKGGPADKAGVEAGDVILRFDGKPVNSSEDLPRIVGATRPGSKAS
                     MQIWRNKQSREAQVVVSEMQDDRAGRQAPRRGGGKPPVTAPSQYGLTLSELSESQRGE
                     LKVSGGVLVENSQGAAARAGIRRGDVILAVNNQDVKSVEQFNQMMGQFDKGRIVALLV
                     RRGANSLYIPFRVDGN"
     gene            3524..3715
                     /locus_tag="AC158Q_10655"
     CDS             3524..3715
                     /locus_tag="AC158Q_10655"
                     /inference="COORDINATES: protein motif:HMM:NF017574.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HIR3340276.1"
                     /translation="MIEALRGLQGLFHFEIAVVDIDADPELVRRHGESVPVLTHGGRE
                     LCHNRLETSIVTAYLSKIR"
     gene            3765..>4300
                     /locus_tag="AC158Q_10660"
     CDS             3765..>4300
                     /locus_tag="AC158Q_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR3340277.1"
                     /translation="MQHIRNFSIIAHIDHGKSTLADRFIQLCGGLADREMEAQVLDSM
                     DLERERGITIKAQTAALDYRAKDGQQYLLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTATEQGVEVIPVLNKMDLPQVEPERVIAEIEDIVGIPAQDALR
                     VSAKSGEGVVDILEAVIHR"
BASE COUNT          870 a         1310 c         1352 g          768 t
ORIGIN      
        1 actcgaccga ggtcgaggac gtgacgcagg aggccttcat caaggcctac cgggcgttgc
       61 cgacgttccg cggcgatagc gctttttaca cgtggctgta ccggataggc atcaacaccg
      121 ccaagaacta cctggtggcg atggggcggc gggctccgac caccacggag atagacagcg
      181 aggaagcaga gggcttcgag gacggcgacc agttgcggga cctcaacacg ccggaggacg
      241 agttggcgag caggcaggtc gccgagaccg tgaaccaaac actgggcgag ttgcctgagg
      301 agttgaggac cgcgattacc ctgcgggaga tcgagggcct gagctacgag gacatagcca
      361 atataatgaa ctgcccgatc ggcaccgtgc ggtcacgtat tttccgggca cgggaggcga
      421 tagcgacgaa attgagaccg ttgctgggca cacagcggga caggagatgg tgagatggac
      481 aagatatctg cattgatgga cggcgaattg gacgagcatc agacccggca gctgctcgtc
      541 cgcctgaaac aggatgagga actgctccag tcctggcata ctttccacct gatcggcgat
      601 gcattgcgtg gcgaacaccc gctgtcgcat gacttcgacc ggcggttgac cgcccggctt
      661 gccggagagc cgacggtgat cgggcggcgg cgcaacgtga ccaaacgcgt cgtcacttac
      721 gccctgtccg ccgccgcttc cttgtccgct attgccctcg tcggctgggt cgctctgtca
      781 gacaatccgc tcggtcccca acctgaactc gccaaggctc ccaaaccgcc tgctgtggcg
      841 gccccgtcgg cgcagctggc cagcgtgccc agtgaaggaa cgatgaacga gtacctgatc
      901 gcgcatcagg agttctcgcc gagcacggcc atccaggggc ttgcacccta catccgcagc
      961 gtatccagca cgcaaccggc acaggggcgc tagtgccgtt ccggttttgc cgttttctgc
     1021 ccatcattgc gctgggttta acggccccat ccctcgctgc cgacgccggc acaaagcagg
     1081 acggcgtgaa ctggctcaag aagatggcca gcgcctcgcg cgagctgaac tacagcggga
     1141 ccttcatcta tcgtcacggc taccgtaccg agacttcccg tgtggttcat tacgtcaatg
     1201 ccgccggcgg ggaattcgag aggatggaaa cgctcgacgg gccgatacgc gaggtgatac
     1261 gcaccaacga ccaggtcgtc tgctacctcc ccaaggccaa gacggtcctc atcgagaagc
     1321 gcggcaagcg ccagtttccc gccatgctgc ccgaccggct gaccggcgtg tccgaaaact
     1381 acacggtaca cgtcggcgag atggaccggg tcgcgggcca cgattgccag gtggtcgtgc
     1441 ttgagcccaa ggacaggctg cgttacgggc atcaatactg cgccgatgcc ggttcgggat
     1501 tgccattgcg cgcgcgcacg ttcaacgaaa aaaaggaacc gctggagtca tttgtattca
     1561 ccgaactggt gatcggcggc agcttcaacc gcgacaaggt gaggtcgcgc tatgccgcaa
     1621 agagccgtga ctggaaggtg gaccactcgg ggtatccgat caacgaggtt gccacggata
     1681 cgggatgggt gctgacacgg cagccggccg gattcaggaa gctgaccgag ttgaagcggt
     1741 cgatcgcggg tcgtgccttc gcggtgtcgc atatcgttta ttccgacgga ctggccgcta
     1801 tctcggtatt catcgagccg ctgcccaagg tccgtattgc ccagcaactg tcgcatcagg
     1861 gagcggtcaa tatatatatc cgccccatcg cggatcacat ggttaccgtg ctcggcgaag
     1921 cgcctgccgc caccgtgatt caaatcgcca attcgctcga actcaaaggg gccgcgctcc
     1981 ggtagtccgt cgcccctgca tgatcagtca ttaccaataa caagccggaa tcatcgggat
     2041 tgcctatgtt caagcgccag ttgctgcgca tcctgtttgc gctgtgcgtc gtcctgccag
     2101 tcgcctccgc ggcgcaattg cccgatttca ccgaactcgt ggagaaacag ggcgcggcgg
     2161 tcgtgaacat cagcaccacg cagtcggtgc gcaatcccct gctgccgcaa gtacccaacc
     2221 tgcaggaaga cgaccccttt tatgaatttt tccgccgctt cataccccag ccggggccgc
     2281 gcgagttcca gtcgcagtcg cttggctccg gcttcatcat cagccaggac ggctatatcc
     2341 tgaccaacgc tcacgtggtc gagacggccg aagaaatcac cgtcaagctg accgacaagc
     2401 gcgaattcaa ggccaaggtc atcggatccg accgtcgcac cgacattgcc ctcataaaga
     2461 tcgaatccgc cagcctgccg gcagtgcgat tcggcgatcc gaaccgcctg aaggtcggcg
     2521 aatgggtgct ggcgatcggc tctccgttcg gcttcgagaa cacggtcacc gcaggcatcg
     2581 tcagcgccaa ggggcgctcg ttgccgcagg agaattacgt gccgttcatc cagaccgacg
     2641 tagcggtcaa tccgggaaat tccggcggtc cgctgttcaa cttgcgcggc gaggtggtcg
     2701 gcatcaattc gcagatctac agccgtaccg gcggattcat gggcctgtcc ttcgcgatcc
     2761 cgatcgacgt tgcgaacgac atcgcgcaac agttgcgcac cacgggcaag gttacccgcg
     2821 gccgcattgg cgtcgtcatc cagccggtta ccaaggaact ggccgacggc ttcgggctgc
     2881 ccagggccca gggcgcgctg gtcaattcgg tggaaaaagg cggaccggcg gacaaggcgg
     2941 gggtcgaggc tggcgacgtg atattgcgat tcgacggcaa gccggtgaat tcctccgagg
     3001 acctgccgcg aatcgtcggc gcgacccggc cgggttccaa ggcgtcaatg caaatctggc
     3061 gcaacaagca gtctcgcgaa gcgcaggtag tggtgtcaga gatgcaggat gatcgtgcgg
     3121 ggcgccaggc accaaggcgc ggcggcggca agccccccgt gacggccccc agccagtatg
     3181 gcctgaccct ttccgagttg agcgaatccc agcgcggcga gctcaaggta tccggcggtg
     3241 tgctggtcga aaattctcag ggtgcggcgg cgcgagccgg gatccgccgc ggcgacgtga
     3301 ttctggcagt caacaaccag gacgtgaagt cagtcgagca gttcaaccag atgatggggc
     3361 agttcgacaa ggggcggatc gtcgcgctgc tcgtgcgccg cggcgccaat tcgctctaca
     3421 ttccgttccg cgtggatgga aattgagcgc aaattgagtg gcagccgaat cgcccctgtt
     3481 actgaccgtc tacagccgcc cttattgcca cttgtgcgat gaaatgatcg aggccctgcg
     3541 gggattgcag ggtctttttc atttcgagat cgcggtcgtg gacatcgacg ccgacccgga
     3601 actggtacgg cggcacggtg aaagcgtgcc ggtattgacg catggcgggc gcgaactctg
     3661 ccataaccgg ctcgaaactt cgatcgttac tgcgtatctt tcaaaaatcc gataaaatac
     3721 acagctttac gtcattggaa gggcactgac gggtgccctt tttcatgcag catatccgca
     3781 atttctccat catcgcccac atcgaccatg gcaagtcgac gctggccgac cgcttcatcc
     3841 agctgtgcgg cggactggcc gaccgcgaga tggaggcgca ggtgctcgac tccatggatc
     3901 tcgagcgcga gcgcggcatc accatcaagg cgcagacagc ggcgctcgat taccgcgcaa
     3961 aggacggcca gcaatacctg ctcaacttga tagatacacc cgggcacgtc gatttctcct
     4021 acgaagtgtc gcgctcgctg tcggcctgcg aaggcgcgct gctggtcgtg gatgcttcgc
     4081 aaggcgtcga ggctcagacg gtggcgaact gctacaccgc caccgagcag ggcgtcgagg
     4141 tgataccggt gctcaacaag atggacctgc cgcaggtcga gccggagcgg gtgatcgcgg
     4201 agatcgagga catcgtgggg ataccggcgc aggacgcgct gcgcgtcagc gccaagtcgg
     4261 gggaaggcgt cgtcgacata ctggaagcgg tgatccatcg
//
LOCUS       DBNGSG010000410         4860 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2849461, whole genome shotgun sequence.
ACCESSION   DBNGSG010000410 DBNGSG010000000
VERSION     DBNGSG010000410.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4860)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2849461"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="AC158Q_10665"
     CDS             complement(<1..348)
                     /locus_tag="AC158Q_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340278.1"
                     /translation="MRPLHNEPLARNTAEPALVQRLHDGTDNRTPGAGKAMKKPAFYN
                     EMYEEDGRVRPHYEPYARWLEGTTVEHLLQKQREADALYTRLGITFAVYGDESGVERA
                     IPFDIMPRVMQPDA"
     gene            604..2745
                     /locus_tag="AC158Q_10670"
     CDS             604..2745
                     /locus_tag="AC158Q_10670"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIR3340279.1"
                     /translation="MESLGQAARECVRDALAVAEPWYRNAVLASGEPALGHAVGAAAI
                     VSELRLDGEAVAAALLASVVQAEPARLRAVAERCGTVVAGLVEGVAKMAQIHALSSRS
                     LSAPAGGQAAQLEALRKMLLAMVQDTRVVLVKLADHTQELRFAARSDDAPTRRGTALL
                     TNEVFAPLANRLGVWQLKWEMEDLAFRILEPETYRRIARMLDEKRGDRERYIEGIVAR
                     LRNELARAGIAAEVSGRPKHIFSIYKKMNTKSTDFKSLYDVRAVRILVQDARNCYAAL
                     GLVHNLWTPIPREFDDYIAKPKANQYRSLHTAVIGPQDKALEVQIRTYEMHQHSELGV
                     AAHWRYKEGLRGDPDYDRKIAWLRQVLEWKDAVGDAGELAERFRTELFDDTIYVMTPQ
                     GRVIALEKGATPVDFAYHVHTELGHRCRGARVNGAMVPLNTPLANGQQVEIVAAKQGG
                     PSRDWMNPELGYIRSAGARAKIRQWFNRQNVEAAVAQGRTVVDRELRRQGMTALSLDR
                     LAAQFGHAKPENLLADIGRGEIRGRQLEQGIRALDPRAGAPAAASRDEAPDVPARRGR
                     SASRRGSVLVVGVDRLLTVPAKCCKPAPPDAIVGFVTRGRGVTLHRAACASLKRLDAK
                     RRVAAEWGEAGDAAFAVDVEVIAGRRAGLLRELSEVLAREKVRIVGSRSTEADANVRL
                     RYTLEVANLGQLTRVLALMREVRGVARAARR"
     gene            complement(2856..3677)
                     /locus_tag="AC158Q_10675"
     CDS             complement(2856..3677)
                     /locus_tag="AC158Q_10675"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: protein motif:HMM:NF013706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008684 - 2-oxopent-4-enoate hydratase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HIR3340280.1"
                     /translation="MYFSRIVLNELLEALDQGKVIPSVVERNPHFDGDAAYKVAGEIV
                     RLRRARGEKTIGRKIGFTNRGIWAEYGATAPIWAHVYDRTVQFAAGHRATVSLKGSVQ
                     PRIEPEIAFKLKAPLRAGVNHPALVLEAIEWLAPSFEIVDCHFADWKFGPADSVADFS
                     FHWRLVVGEPRAVHGDEISGLVEQLRDCNVTLSRNGQFADRGVGANALGHPAAALAFL
                     ADILMRQPQFEPLAAGEVITTGTLTAAMPIHSGETWSSRYEGLPGVTGLTLRFTD"
     gene            complement(3680..4468)
                     /locus_tag="AC158Q_10680"
     CDS             complement(3680..4468)
                     /locus_tag="AC158Q_10680"
                     /inference="COORDINATES: protein motif:HMM:NF015722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HIR3340281.1"
                     /translation="MKCCCAALALAAASWAAAPAADDMAAIPAGPFTMGSDEGPADER
                     PAHRLTLAAYLIDRYPVTNARFAEFLNAIGPFNARQERLFDFDDPDARVHRVHGKWVA
                     DAGFEQHPVVEVTWAGARDYCAWRGKRLPSEAEWEKAARGTDGRRYPWGNSTPDRSRA
                     QFSARFNETAPVGAFPAGASPYGVRDMAGNAWEWVSSAYRPYPYDAKDGREDLTAGAV
                     RGTRGGGHDSPADGITATQRGRNLSRNPASGHHNIGFRCANRGD"
     gene            complement(4465..4752)
                     /locus_tag="AC158Q_10685"
     CDS             complement(4465..4752)
                     /locus_tag="AC158Q_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010096484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HIR3340282.1"
                     /translation="MAAYVIVDMTVTDPVKIEEYRKLAGATVAAMGGKFLARGGTTEV
                     FDGDWKPQRIVVIEFPGLEQAKRWRHSSEYGKACEIRDRAARTRMIVVEGA"
BASE COUNT          821 a         1518 c         1689 g          832 t
ORIGIN      
        1 ggcgtcgggc tgcatgaccc ggggcatgat gtcgaacgga atggcgcgct cgacgccgga
       61 ttcgtcgccg tagacggcga aggtgatgcc gagccgggta tagagcgcat cggcttcgcg
      121 ctgcttctgc agcaggtgct cgaccgtggt gccttccagc caccgggcgt agggctcgta
      181 gtgcgggcgg acgcggccgt cttcttcgta catctcattg tagaatgcgg gctttttcat
      241 ggccttgccc gcgccggggg tgcggttgtc cgtgccgtca tgcaaccgtt gtaccagggc
      301 tggttcagcg gtgttccggg cgagcggctc gttgtgaagt ggccgcatgg ccggggtgga
      361 cgtagatgcc ggttttcgcg ccatcctggg cccgcaccaa aacaggggtg aggatcaaag
      421 atacgcctga aagcggtgcg gagccaacgg cagggacggg acagggatcg ggcgcgattg
      481 gcgagtgcgg ccaggaagca cggaaatggc ggaattagat ttatggcgaa ggcacctgcg
      541 ttaaaactgg tgcgcccgac ggcgcgcgat gtccccgtga ccggtgcgaa aaccgacgca
      601 tggatggagt cgctcggtca ggccgcacgg gaatgcgttc gtgacgcgct tgccgtggcg
      661 gagccgtggt accgcaacgc cgtgctcgcg agcggggagc cggcgctggg gcatgccgtc
      721 ggcgcggcgg ccatcgtttc cgagttgagg ctggatggcg aggcggttgc cgcggcgctg
      781 cttgcgtcgg tggtgcaggc cgaacccgcg cgccttcgcg ccgtggcgga gcgctgcgga
      841 accgtcgtgg ccgggctcgt cgagggcgtg gcgaaaatgg cgcagatcca tgcgctgtcg
      901 agccgatccc tgtccgctcc agccggcgga caggcagccc agctcgaagc gcttcgcaag
      961 atgctccttg ccatggtgca ggacacgcgc gtcgtgctgg tgaagctggc cgatcacacg
     1021 caggaactgc gctttgcggc caggtccgac gacgcaccaa cgaggcgtgg gaccgcgctg
     1081 ttgaccaacg aggtcttcgc gccgctcgcc aaccggctgg gcgtgtggca gctcaagtgg
     1141 gagatggagg acctcgcatt tcgcatcctc gagcccgaga cctaccggag aatcgcgcgg
     1201 atgctggatg aaaagcgcgg ggaccgggag cgctacatcg agggcatcgt cgcgcgcctc
     1261 aggaatgaac tggctcgcgc cggcatcgcg gccgaggtta gcggccgtcc caagcacatc
     1321 ttcagtattt acaagaagat gaatacaaag tccactgatt tcaaatcgct ttatgatgtc
     1381 agggcggtgc ggattttggt gcaggacgcc cgcaattgct acgctgcgct ggggctcgtg
     1441 cacaacctct ggacaccgat cccgcgggag ttcgacgact acatcgcgaa gcccaaggcg
     1501 aaccagtacc gctcgctcca taccgcggtg atcgggccgc aagacaaggc gctggaagtg
     1561 cagatccgca cctacgaaat gcaccagcat tccgagttgg gggtggcggc acactggcgc
     1621 tacaaggaag gtttgcgcgg ggacccggat tacgaccgga agattgcctg gctgcggcag
     1681 gtcctggagt ggaaggacgc ggtcggggac gcgggcgagc tggccgagcg cttccgcacc
     1741 gagctgttcg acgacacgat ctacgtgatg acgccgcagg gccgcgtgat cgcgctggaa
     1801 aaaggcgcca cgccggtgga ttttgcctac cacgtgcaca cggaactggg gcatcgctgc
     1861 cgcggcgcca gggtgaatgg cgccatggtg ccgctcaaca cgccgctcgc gaatggccag
     1921 caggtcgaga tcgtagcggc gaagcagggc ggacccagcc gcgactggat gaacccggaa
     1981 ctcggttaca tccgaagcgc gggcgcgcgc gccaagatcc gccagtggtt caaccgccag
     2041 aacgtcgagg ccgcggtggc gcagggcagg accgtggtgg acagggagtt gcggcggcag
     2101 ggcatgaccg cgttgagcct cgacaggctg gccgcacagt tcggtcacgc gaaaccggaa
     2161 aacctgcttg ccgatatcgg gcgcggcgaa atccgcggcc gccagctcga gcaggggatt
     2221 cgagcgctgg acccgcgcgc cggcgcgccg gccgccgcat cccgggacga ggcgcccgat
     2281 gtgccggcgc gcaggggacg gtccgcttcg cgcaggggca gcgtgctggt cgtgggcgtg
     2341 gaccggctgc tcacggtgcc ggcgaaatgc tgcaagccgg ctccgcccga cgccatcgtc
     2401 ggattcgtca cgcgcggccg cggcgtcacc ctccaccgcg cggcctgtgc cagcctcaag
     2461 cggctggatg ccaaacggcg ggttgccgcc gaatggggcg aagccgggga tgcggccttt
     2521 gcggtggatg tcgaggtcat tgcgggcagg cgcgccgggc tgctgcgcga actctccgaa
     2581 gtgcttgcac gcgagaaggt ccgcatcgtt ggctcgcgct cgacggaggc cgatgccaac
     2641 gtgcgcctgc gttatacgct cgaggttgcc aatctcggcc agctcactcg cgtgctcgcg
     2701 ctgatgcgtg aagtgcgcgg ggtcgcgcgt gccgctcgca ggtagttaat ggacaggatt
     2761 aacaggataa acaggattga caagattcag tcaacactcc aggtattcct gtttttgatc
     2821 taatcctgta aatcttgtaa atcctgtcta tttgttcaat cagtaaagcg cagcgtgagg
     2881 ccggtgacgc ccggcaatcc ctcgtagcgc gaggaccagg tttcgccgga atggatcggc
     2941 atggccgcgg tgagcgtgcc ggtggtgatc acttctcccg ccgcgagcgg ctcgaattgc
     3001 ggctggcgca tcaggatgtc ggcgaggaag gcgagcgccg cggcgggatg gcccagcgcg
     3061 ttcgcgccga cgcctcgatc cgcgaattga ccattcctgc taagcgtgac gttgcagtcg
     3121 cgcaactgct cgacgagccc ggaaatctcg tcgccgtgaa ccgcgcgggg ctctccgacg
     3181 accagccgcc agtgaaagga aaagtcggcg accgaatcgg cgggcccgaa tttccagtcg
     3241 gcgaagtgac agtccacgat ctcgaagctg ggcgccagcc actcgatggc ttcgagcacc
     3301 agcgcggggt gattgacgcc cgcccgcagc ggggccttga gcttgaacgc gatctcgggc
     3361 tcgattcgcg gctgcacgct ccccttgagc gagacggtgg cgcgatggcc ggcagcgaac
     3421 tgcacggtgc gatcgtagac gtgcgcccag atcggcgcgg tggcgccata ttcggcccag
     3481 atgccgcggt tggtgaagcc gatcttgcgc ccgatggtct tctcgccgcg cgcgcgccgc
     3541 agcctcacga tttcccccgc gaccttgtat gccgcatccc cgtcgaaatg cgggttgcgc
     3601 tcgacgacgg acgggatgac cttgccctgg tccagcgctt cgagcagctc gttgagcacg
     3661 atgcgtgaga aatacatggt caatcgcccc gattagcgca gcgaaagccg atattatggt
     3721 ggcccgatgc cgggttgcgt gaaaggttgc gaccgcgctg cgtggctgtg atcccgtccg
     3781 ccggcgaatc gtgcccgccg ccgcgcgtgc cgcgcaccgc gcccgcggtc aaatcttccc
     3841 ggccgtcttt cgcatcgtaa gggtagggac ggtatgcgct ggaaacccat tcccacgcat
     3901 ttcccgccat gtcccgcacg ccgtagggac tcgcgcctgc tggaaatgcg ccgaccgggg
     3961 cggtttcgtt gaagcgcgcg gagaactgcg cgcgcgaacg gtcgggagtc gagttgcccc
     4021 agggatagcg gcggccatcg gttccgcgtg ccgctttttc ccattccgcc tcggacggca
     4081 gccgcttgcc gcgccaggcg cagtaatcgc gcgcgccggc ccaggtcacc tcgacgaccg
     4141 ggtgctgttc gaacccggca tcggcgaccc atttgccatg tacgcgatgc acccttgcat
     4201 caggatcatc gaagtcgaac aggcgttcct gcctggcgtt gaaggggcct atcgcgttca
     4261 ggaattccgc gaagcgggcg ttggtgacgg gataacggtc gatgagatat gcggcgaggg
     4321 tcagccggtg cgccgggcgt tcgtcggcgg gcccctcgtc gctgcccatg gtgaacgggc
     4381 ccgcgggaat tgcggccatg tcatccgcgg ctggcgcggc ggcccagctt gccgccgcca
     4441 gagccagcgc cgcgcagcag cacttcaagc accctccacc acgatcatgc gcgtccgggc
     4501 ggcgcggtcg cgtatctcgc aagccttgcc gtattcggac gagtgccgcc agcgcttcgc
     4561 ttgctccagt ccgggaaatt cgatcaccac gatgcgctgc ggtttccagt cgccgtcgaa
     4621 aacctcggtc gtgcctccgc gggcgagaaa tttcccgccc atggcggcga cggtggcccc
     4681 cgccagcttg cggtactcct cgatttttac cgggtcggtg acggtcatat ccacgatcac
     4741 gtaggcggcc atgttacctc cggcgagcgt cgttcaactc actgatttta tttgaagctg
     4801 ccatgctatt ccgctacaat tcgcactccc caggcgcgta gctcagttgg ctagagcgca
//
LOCUS       DBNGSG010000411         5930 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4718300, whole genome shotgun sequence.
ACCESSION   DBNGSG010000411 DBNGSG010000000
VERSION     DBNGSG010000411.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5930)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5930)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5930
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4718300"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="AC158Q_10690"
     CDS             <1..498
                     /locus_tag="AC158Q_10690"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HIR3340283.1"
                     /translation="HSVLTRRLDDIPEVSGTDFLKLDVQGGEIMVLNGAVRTLRDVLV
                     IHAEAEFLPMYKNQPLFSDIDPWMRAHGFVLHKIPILGVRALYPFSTTDAAAANQVLW
                     SDVVYVRDFMVLDRLSEAQLLKLAAILHANYESYDLAGVALGVVDRRTGSDLQKQYLD
                     SLMAA"
     gene            495..2135
                     /locus_tag="AC158Q_10695"
     CDS             495..2135
                     /locus_tag="AC158Q_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340284.1"
                     /translation="MRIELRPAPADILAAFFSLAFAAACTAFAWQPRLASLADDSVSY
                     LVMAQAFSPWQPASAPVAEAFAREAFYPPLYPMVLALSGGAHDMAFAHAITALLLAAC
                     LPALYVLGERWLGSKWAAAGAVAVTASLPSLWIHVKGMLSEPLFCLLLISTMCILETG
                     QGGRTRPWTIAALMAAMVLTRTVGLVVVAAYALWALSRRGQPLSQRIHALLPAFFAAL
                     AYAAWVLMRPADTADDYMRIVFERGQAILAAGNPWAALASSITRQANSLGEAWVGALL
                     LFWVEGRPLRVVLAGAVGALAAAGLVMRLAAGKPDAWMMTAYLATFLVWPFYDQMTRF
                     LFPALPVLVLYAFWAGNAALQVLGRPPALAHGLLVLLLLSLAAPAMGFIHQRAKAGGR
                     HVEMTEWYRTPDVDNARARAQIHLDLMEDMEAIRRLTEPQDRIMWVVPSYIALLADRR
                     GVSAPAAGLLPSDYRRAVHDANPDYLYLSTYHPRDTVRETAWRTGMEAMAGHAEIIHA
                     RKPGEGSAIGSALLKIRGAGAKGLLKVRASAGHDHGAH"
     gene            2137..2529
                     /locus_tag="AC158Q_10700"
     CDS             2137..2529
                     /locus_tag="AC158Q_10700"
                     /inference="COORDINATES: protein motif:HMM:NF016060.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HIR3340285.1"
                     /translation="MLRGVLGEGTRYFAASAAAFAVDFGLYVALVRLAEVHYLVAAPI
                     AFALGLATIYALSIRWVFADRRLTDARIEFAVFASIGIGGMALNQGVLYAGVELFSLS
                     YELAKLASTGIVFCFNFVLRKLLLFTRY"
     gene            2539..4119
                     /locus_tag="AC158Q_10705"
     CDS             2539..4119
                     /locus_tag="AC158Q_10705"
                     /inference="COORDINATES: protein motif:HMM:NF005546.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3340286.1"
                     /translation="MPLRVVIIGAGPAGLTAAHELAAAGVRDIVVLEATSDVGGLSRS
                     VNYKGNRIDIGGHRFFSKSDWVMNWWLRMLPLAAAPSLAGELNLAYQGMRRALPEAPT
                     ASESEPRVMLVRRRLSRIYFEGKFFDYPLKADFETAFKLGLWRCATFAASYLRAKLFP
                     RRPELSLEDFFVNRFGDRLYRQFFKEYTEKVWGTPCDRISAQWGAQRVKSLSIGTALR
                     HALRKLFRRRADDFGAAQQTSLIERFLYPKYGPGLMWEVTAERLRERGVKIVFDAAVK
                     HIDHSDRRIRAVRTVGAGGAEQHYEADCFVSTMPVRELVLGLSPEAPSRPRNVAAGLE
                     YRDFITVGLLLRRLMRTPGGKGPLNLVPDNWIYVQEPGVKVGRLQVFNNWSPYLVADP
                     NTVWIGMEYFCSEGDELWEAADDELKQLGIREMQKLRLANPEDHLDSVVIRMPKAYPG
                     YFGTYGSFDELRGYLDAFPNLFLAGRNGMHRYNNQDHSMLSAKLAAAAILAGSSDKAP
                     IWNVNIDDEYHEEDSAGR"
     gene            4116..5300
                     /locus_tag="AC158Q_10710"
     CDS             4116..5300
                     /locus_tag="AC158Q_10710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3340287.1"
                     /translation="MSNVPPSVVRADEPVAVSAVIPCLNEERSIGVCVEKARRAFLGL
                     GVAGEVVVADNGSTDRSADIAREAGARVVLQPVKGYGAALRKGIEAARGRVIVMGDAD
                     DSYDWSSIGPFVRKIDEGYDLVVGNRFQGGILPGAMPLLHRYVGNPLLSAFTRLAFGV
                     RVGDVHCGRRALTREACARMGAGTAGMEFATEMVASAAHQGLRITEVPTRLYPDKRGR
                     PSHLRTFRDGWRHLRFILTYAPDYLYLAPGAGLLGLGLLLQAVLIAGPVSLGGLSFGI
                     HYLALGAMLSLAGFNVINLGVLAKAIMAQRYPALKSRTLRLLTGRYTLESGLIAGALL
                     MLTGLIVDGVILGNRLTNPGAAMEDTVHLAFVATTMIVLGLNLMFSSFLLAMVTGARD
                     RN"
     gene            complement(5304..>5930)
                     /locus_tag="AC158Q_10715"
     CDS             complement(5304..>5930)
                     /locus_tag="AC158Q_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340288.1"
                     /translation="GRVLGWTSSTGLSVHLLDLAKLWLWAVPALLVCALLGARRLRED
                     RGIWLALAGSALLTYFVYFVVRFDQGHGWGFRYFHSAWLVLPLFAVRALATPGREPLA
                     GYLAGCALLSLVVLTGFRALQVEQFVARHLSQLPVAASGKARVRIVNTANGYYAWDLI
                     QNDPFLRGPITLGSRGAQADAAMMAARFPQHEQLSEDRRGSVWGTPAR"
BASE COUNT          951 a         1900 c         1996 g         1083 t
ORIGIN      
        1 cacagcgttc tcaccaggcg actcgacgac atacccgagg tgtccgggac ggactttctc
       61 aagctggacg tgcagggcgg cgaaataatg gtacttaacg gagcagtccg gacactgcgc
      121 gacgtgctgg tcatacatgc ggaagccgaa tttcttccga tgtacaagaa ccagccgctg
      181 ttttccgata tcgacccgtg gatgcgcgcc catggatttg tgcttcacaa gattccaatc
      241 ctcggcgtgc gggcgcttta tccattctcg acgaccgacg cggcggcggc gaatcaggtg
      301 ctgtggagcg acgtggtcta cgtgcgcgat ttcatggtgc tggaccgcct gtcggaagcg
      361 caattgctga agctggcagc gatcctccac gcaaattacg aatcgtacga cctcgcgggg
      421 gtggcgctgg gcgtggttga ccgccggacc ggctcggacc tgcaaaaaca gtatctcgat
      481 tcgctcatgg ccgcatgagg atcgagcttc gtcccgcgcc ggcggatatc ctggccgcgt
      541 ttttttcgct ggccttcgcg gcggcatgca cggccttcgc ctggcagccc aggcttgctt
      601 cgttagcgga cgactccgtc agctatctcg tgatggcgca ggccttctcg ccgtggcagc
      661 cggcatcggc gccggtcgcg gaagcgttcg cgcgcgaagc gttttatccg ccgctctatc
      721 ccatggtgct tgcgctgtcc ggtggcgccc atgacatggc tttcgcccat gcgatcacgg
      781 cgttgctcct cgcggcctgc ctgcccgcgc tctatgtgct gggcgagcgc tggctcggga
      841 gcaagtgggc cgctgccggt gcagtcgcgg tgaccgcgtc actgccgtcg ctgtggatac
      901 acgtcaaggg catgctgagc gagccgttgt tctgtctgct gctgatctcc acgatgtgca
      961 tcctggaaac cgggcagggt ggtcgaacgc ggccatggac cattgctgca ctgatggccg
     1021 cgatggtcct gaccagaacg gtagggctgg tggttgtcgc ggcatacgcg ctctgggcgt
     1081 tgtcgcggcg cggccaaccg ctgtcgcaac ggattcatgc gctgctgccg gcgttcttcg
     1141 cggcgcttgc ctatgccgcg tgggtgctga tgcgtcccgc ggatacggcc gatgactaca
     1201 tgcggatcgt attcgagcgc gggcaggcga tactcgccgc gggaaatcct tgggcggccc
     1261 tcgcttccag catcacgaga caggcgaact ccctgggcga ggcctgggtc ggcgccctgc
     1321 tgctgttctg ggtcgagggc cgacccctgc gcgtcgttct cgccggcgct gttggcgcgc
     1381 tcgcggctgc cgggttggtc atgcgccttg cagcgggaaa acccgatgcc tggatgatga
     1441 ctgcgtacct ggcgaccttc ctcgtctggc cgttttacga ccagatgacg cgttttctct
     1501 tcccggcgtt gccggtgctg gtgctctacg cgttctgggc gggcaatgcc gccctgcagg
     1561 tgctcggacg gccgcccgcc cttgcgcatg gattgctcgt gctgctgttg ttgagccttg
     1621 ctgcgccggc gatgggcttc atccaccagc gcgcaaaagc cggcggccgc cacgtcgaga
     1681 tgaccgagtg gtatcgcacg ccggatgtcg acaacgcgcg cgctcgcgcc cagatacacc
     1741 tcgatctgat ggaggacatg gaggcgatcc gcaggctcac cgagccacag gaccgtatca
     1801 tgtgggtagt gccttcctac atcgcgctgc ttgcggatcg ccgcggcgtt tccgcgccgg
     1861 cggcaggcct cctgccctcg gactatcggc gggcggtgca tgacgccaac ccggactatc
     1921 tgtacctgag cacctatcat ccgcgcgata cggtgcgcga aacggcgtgg cggaccggca
     1981 tggaagccat ggccggacat gccgagatca tccatgcgcg caagccgggc gaaggcagcg
     2041 cgatcggctc tgcactgctg aaaatcaggg gcgccggcgc gaaggggctg ctgaaagtcc
     2101 gggcaagtgc aggtcatgat cacggcgcgc actaacatgc tgcgcggcgt actgggcgag
     2161 gggacgcgct acttcgccgc ctcggccgcc gcgttcgcgg tcgatttcgg tctttacgtt
     2221 gcattggttc gcctggccga ggttcattat ctcgttgcgg cgccgatcgc gtttgcgctc
     2281 gggctggcga cgatctacgc gctgagcatc cgctgggtgt tcgcagaccg caggctcacg
     2341 gatgcccgca tcgagtttgc cgtcttcgct tccattggca tcggcgggat ggcgctcaac
     2401 cagggggtgc tctatgccgg cgtcgaactg ttttccctgt cctacgagct cgccaagctc
     2461 gcttcgaccg gcatcgtatt ctgtttcaat ttcgtcctgc gcaagctcct cttgttcacg
     2521 aggtactagg cccgcaccat gcctttacgc gtcgtcatca tcggcgccgg gcccgcgggc
     2581 ctgacggcgg ctcatgagct ggcggctgcc ggggtgcgcg atatcgtcgt gctcgaggca
     2641 acgagcgatg tgggcggcct gtcgcgctcg gtgaattaca agggcaaccg catcgatatc
     2701 ggcggccacc gctttttctc caagtcggac tgggtcatga attggtggct gcgcatgctg
     2761 cccctggccg ccgctccgtc gcttgccggg gagctgaatc tcgcctacca gggcatgcgg
     2821 cgcgcgctgc ccgaggcacc gacggcgagc gaatccgagc cccgggtgat gctggtacgc
     2881 aggcgcctgt cccgcattta cttcgaaggc aagttcttcg actatccact gaaagccgac
     2941 ttcgagacgg ctttcaagct cgggctgtgg cgctgcgcga cgttcgcggc aagctacctc
     3001 cgggcgaagc tgttcccgcg ccgcccggag ttgagcctcg aggatttttt cgtcaatcgc
     3061 ttcggcgacc gcttgtacag gcagtttttc aaggaatata cggagaaggt gtggggcacg
     3121 ccgtgcgacc ggatcagcgc gcaatggggc gcgcagcgcg tgaagagcct gtccatcggc
     3181 acggccctgc gccatgcgct gcgcaagctg ttccgccgcc gcgccgatga cttcggggcg
     3241 gcgcagcaga cctcgctgat cgagcgcttc ctttatccca agtacgggcc ggggctcatg
     3301 tgggaggtca cggccgagcg gctgcgggag cgcggcgtga agatcgtatt cgacgcggcc
     3361 gtgaagcaca tcgatcacag tgatagacgg atccgcgcgg tgcgcacggt cggggcaggc
     3421 ggcgcggagc aacactacga ggcggactgc ttcgtgtcca ccatgccggt gcgcgagctc
     3481 gtgctggggt tatcgcccga agcgccgtcc cgcccccgga acgtggctgc cggcctcgag
     3541 taccgcgatt tcatcactgt cgggctgctg ctgcggcggc tcatgcgcac ccccgggggg
     3601 aaggggccgc tcaacctggt gccggacaac tggatctacg tccaggagcc cggggtcaag
     3661 gttggccggc tgcaggtctt caacaactgg agcccttacc tggtagcgga cccgaacacc
     3721 gtgtggatcg gcatggagta tttttgcagc gagggcgacg agctgtggga ggctgcggac
     3781 gacgagttga aacagctcgg aatccgcgag atgcagaaac tgcgtcttgc caatcccgag
     3841 gaccatctgg acagcgtggt catccgcatg cccaaggcct acccgggcta tttcggcacc
     3901 tacggctcct tcgacgaatt gcgcggatac ctggatgctt tcccgaatct tttcctggcg
     3961 gggcgcaacg gcatgcaccg ctacaataac caggatcact cgatgctttc ggccaagctc
     4021 gccgccgcgg cgatcctcgc gggcagcagc gacaaggcgc cgatctggaa cgtgaacatc
     4081 gacgacgaat atcacgagga agattccgcc ggccgatgag caacgtgccg cccagcgtag
     4141 tgcgcgcgga cgagccggtc gcggtctcgg cggtgattcc ctgcctcaac gaggaacgaa
     4201 gcatcggcgt gtgcgtcgag aaagcgcggc gggcattcct gggcctgggc gtggcgggag
     4261 aagttgtcgt ggccgacaac ggctcgaccg accgctcggc ggatattgcg cgggaggccg
     4321 gcgcccgcgt cgtgctgcaa ccggtcaagg ggtacggcgc cgcgttgcgc aagggcatcg
     4381 aggcggcacg cgggcgggtc atcgtgatgg gcgacgcgga cgattcctat gactggtctt
     4441 cgatcgggcc tttcgtccgc aagattgatg aaggctacga cctggtcgtg ggcaaccgtt
     4501 tccagggcgg catcctgccg ggcgccatgc cgctactgca ccgctacgtg ggaaatcccc
     4561 tgctttccgc gttcacgcgg ctcgcgttcg gagtgagggt cggcgacgtg cattgcggaa
     4621 ggcgtgcctt aacccgggag gcctgcgcgc gcatgggggc tggcaccgcg ggcatggagt
     4681 tcgcgaccga aatggtcgcg agcgccgctc accaggggct gcgcatcacc gaggtgccga
     4741 cacggctcta tccggacaag cgcggccggc catcgcacct caggacattt cgcgacggtt
     4801 ggcgacacct gcgcttcatc ctcacctacg cgcccgacta tctgtatctc gcgccgggcg
     4861 ccgggctgct cgggctcgga ctgctgctgc aggccgtcct gatcgccggc cccgtgagcc
     4921 tcggcggcct gagcttcggc atccactacc tcgcgctcgg cgccatgctc tcgctggccg
     4981 gcttcaatgt catcaatctg ggcgtgctcg cgaaggcgat catggcgcag cgctacccgg
     5041 cgttaaagag ccgcacgctg agactgctga cgggacgcta taccctggag tccgggctga
     5101 tcgccggagc actgctgatg ctcaccgggc tgatagtgga cggcgtgata ctcggcaacc
     5161 ggctgacgaa tccgggcgcg gcgatggagg acaccgtgca cctcgcgttc gtcgcgacga
     5221 cgatgatcgt gctcgggttg aatttgatgt tcagctcgtt tctgctcgcc atggtcacgg
     5281 gcgcgcgcga tagaaactga agatcagcgg gccggcgttc cccacaccga cccccgccgg
     5341 tcctcggaca actgctcgtg ctggggaaag cgcgcggcca tcattgcggc atccgcctgg
     5401 gcgccgcgcg agccgagggt gatcggcccg cgcaggaagg gatcgttctg gatcaggtcc
     5461 caggcgtaat agccgttcgc cgtgttgacg atgcgaaccc gcgccttgcc ggaggccgcg
     5521 accgggagct gcgacagatg gcgcgccacg aactgctcca cctgcagcgc gcggaagccc
     5581 gtgagaacaa ccaggctcag cagcgcgcag ccggcgaggt agcctgccaa cggctcacgc
     5641 cccggtgtgg cgagcgccct cacggcaaac agcggcagca ccagccaggc ggagtggaag
     5701 tagcggaatc cccagccgtg cccctgatcg aaacgcacca cgaagtaaac gaagtaggtc
     5761 agcaacgcgg atcccgccag cgcaagccag atgccccggt cctcgcgcag gcgccgtgcc
     5821 cccagcagcg cgcagaccag cagcgccggc actgcccaga gccagagctt ggcgagatcc
     5881 aggagatgga cgctcaagcc cgtcgaggag gtccagccga gcacgcggcc
//
LOCUS       DBNGSG010000412        11844 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1662009, whole genome shotgun sequence.
ACCESSION   DBNGSG010000412 DBNGSG010000000
VERSION     DBNGSG010000412.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 11844)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11844)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11844
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1662009"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..718)
                     /locus_tag="AC158Q_10720"
     CDS             complement(140..718)
                     /locus_tag="AC158Q_10720"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013755.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIR3340289.1"
                     /translation="MRSISKEDLNNLPIRRYEGRVSLVETPRELEQAREDIRQERVVG
                     LDTETRPSFRKGEVHLPCLVQAATGQAVYLFQLNRLDVFPALVELLAKPQIVKTGVGL
                     AYDLRQLKLVFPFTVENVVDLGVVARRRNLGQTGVRNLAGMLLEFRIPKGNRTSNWAA
                     PRLTPAQINYAATDAWACRELYLKFETLGLLN"
     gene            835..1188
                     /locus_tag="AC158Q_10725"
     CDS             835..1188
                     /locus_tag="AC158Q_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR3340290.1"
                     /translation="MAISGRWIFTASMDVDADKEALFNEVYDTEHVPHLRKVPGVVSV
                     TRAKLDTLRVTMGGETRTVDTQGKPRYACYVELDSPDVLTGPAWSKAVDAGRWPGEVR
                     PFTRNRQHTLHKVMK"
     gene            1626..2075
                     /locus_tag="AC158Q_10730"
     CDS             1626..2075
                     /locus_tag="AC158Q_10730"
                     /inference="COORDINATES: protein motif:HMM:NF016955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HIR3340291.1"
                     /translation="MLKALKSLFDDAGSRPLPGHERRHLHVAVASLLHEASRVDLRED
                     GTEHAAAQAGLADLFGLTEQEAAAVLAEGRGKARQMTSYFGAVAVVKRDMGQPERVRL
                     IEHLWRIAYADGPLDPYEDHYVRKIGHLLYVTNTDIVLARNRARTGA"
     gene            2170..2865
                     /locus_tag="AC158Q_10735"
     CDS             2170..2865
                     /locus_tag="AC158Q_10735"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR3340292.1"
                     /translation="MPGRNIKVRSREGGEFDCYLALPGASGKAPAVVLASAVHGVDAD
                     VRAIADEFAGHGYIAAAPDLFWRSIPGPLARDDDRTKLRSQPRLEKIRAGEADMADTL
                     AEVRKLPQFNGRAASMGFCYGGPYAILGPKRLGYDAGISCHGTQFLDFLHELDGVTRP
                     VCIIWGDQDNQAPAPVLEAYRKVPARMNNVQVHVVPGILHGYMFPGNRQAFSQSTRDF
                     SIKHALALLGALR"
     gene            2968..4242
                     /locus_tag="AC158Q_10740"
     CDS             2968..4242
                     /locus_tag="AC158Q_10740"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3340293.1"
                     /translation="MRTPPLSLAFAVWGLGAALYLVGFFHRVAPAVITRELAAEFELT
                     AAALGNLSAFYFYSYVILQVPTGMLVDRYGPRRILTLGAVGAAAGALLFAGAHSFALV
                     GAGRLLIGASVGVAFVAMMKLSTHWFHPSRFALVAGLALGTGMVGAASAGAPLRWLAD
                     VFGWRGVILATAAATAALAAAIWLVVRDDPQERGYASYLPAAHHGEPAQSMTGGLARV
                     LGSRNVLLVCVMNGGVSGLVLTFGGLWGVPFLATHYGLSTAAAAALCSLLLLAWGVGG
                     PLLGALSDRLTERKRLYGLGAGVATICWCVILLVPGLPLALLAALLAAAGLASGVIMI
                     GYAYAKESAPAALAGTTGGVINTGNMLGAMLMQPAVGWVLDRAWDGGLEAGLRVYSFA
                     AYRLGFAVLLALLLAAVFVTALTRETHCRQQQ"
     gene            4402..4695
                     /locus_tag="AC158Q_10745"
     CDS             4402..4695
                     /locus_tag="AC158Q_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340294.1"
                     /translation="MLLSRDVLYERKFVKCNPDYDPEKPCVYVGMTGQSPDVRFDKHK
                     AGLKSNRYVRKYGIRLMPELYECYNPMPYGAALDMEIELAIHLRERGYGVWQG"
     gene            5105..5941
                     /locus_tag="AC158Q_10750"
     CDS             5105..5941
                     /locus_tag="AC158Q_10750"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008163432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3340295.1"
                     /translation="MKISRRAFQWLLALCFVSAAGAAAVLAQDAQDTPKPFVPFEGQE
                     GKDVVWVPTPGVLVEKMLDLAKVTPQDYVMDLGSGDGRNIIAAARRGARALGVEYNPD
                     MVELSRREAEKQGVADKAQFVQGDMYEADISQATVMALFLLPDNLEKLTPKFLDLKPG
                     SRMVLNGFAVPGWEADVTERAEGDCGSWCTAMLYIVPARVAGTWRLPQGELRLEQKFQ
                     MLTGTLTRGGNRTPIKDGRLRGEEISFMAGGTKYVGRVKGDAISGANGTWTATRIRSK
                     LQ"
     gene            6022..6468
                     /locus_tag="AC158Q_10755"
     CDS             6022..6468
                     /locus_tag="AC158Q_10755"
                     /inference="COORDINATES: protein motif:HMM:NF024861.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HIR3340296.1"
                     /translation="MLDTNVWLDWLLFGDPDVAPMKAAVAQGRAGIFMDEAGEAELAR
                     VLAYPFQNRTLDAAAQAELIALCGRIARKAEVGSRKANESWLPKCEDADDQKFLELAL
                     TCGAAFLVTRDRALLGLARHKPRPLPYRIVTPAQLAAAFRSDEVIK"
     gene            6770..7000
                     /locus_tag="AC158Q_10760"
     CDS             6770..7000
                     /locus_tag="AC158Q_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340297.1"
                     /translation="MANFDSAAGESGDLFGEKPVTRRGPAIKRRPAPELAVARALCRQ
                     LLRKQASPEKKRIAHLICLNLVSMLKNGSQRP"
     gene            7323..8522
                     /locus_tag="AC158Q_10765"
     CDS             7323..8522
                     /locus_tag="AC158Q_10765"
                     /inference="COORDINATES: protein motif:HMM:NF019640.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3340298.1"
                     /translation="MQKPAMDFSGVGYDEALARARELIPFLREQAPKCEEARRLTPEV
                     MDRLTRAGLFRYLQPKVRGGMELPFVSYFDIPEILARGDISVAWTVDNLASHHRNITW
                     FDPRAQEEVWGANPDAGIASGIAYPQGRGVPADDGITLSGEWNFSSGTDHSDWSLLAC
                     IVRDGDKILDYRFCLLHRSQYEIVDDWHTLGMRATSSKTVRCRDVFVPAYRSVSMWVA
                     REGHSWPGLAVHGNPHYRVPISALGGHGLGACMIGNARAALELAVELVKSRSTNYTGA
                     KMRDFQTVQLRIGAAGVKADAAYTLLRNDCLEAQRVYEEGGNLDIETRLRYKRNAAQA
                     ARWCVEAVDSLHEMAGANGIYDHYPLQRVFRDMRAAAGHFSFSTAAQLPPWGLVAMGG
                     EYKSPTL"
     gene            complement(8721..10409)
                     /locus_tag="AC158Q_10770"
     CDS             complement(8721..10409)
                     /locus_tag="AC158Q_10770"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR3340299.1"
                     /translation="MTSQRAPMHAGEAVAEALREEGVERVYSVPGSHIHPIYDGLSRV
                     KSIRFVTCKQEPNVSLMADAYGRLTGKPGVCVVTAGPGGLNSMAGVAQAYGAASPMVH
                     IGGAVPLKADLEAFHGVDDPAFVHEMFKKITKWSARVESIEHIPAVMAKAFHVARSGR
                     PGPVHVELPRLSDYSEHILQEERAVLPAYKPAPAVVAKPDPKDVDRFAKRLMESRAPV
                     IAAGKGVIRKGALRELAELSMKLQAPVVFAQDAIGVIPETHPFFAGHFQHYRSHPLCV
                     AALERTDLVLAVGLRAGTAEMAELREKAPERALILVGFDDAANARYQGADQRVADPGL
                     FLTALLERLGSYQRPRNEALIRQMAEARAAVRASIAAQGEPHRDDNPIYPGVLMDAMN
                     TVLGDDAVVASDVGNCQMWARTYRRIATPESFMQSGVWNAMSYGLPTAIVAKMEFPGR
                     DVVALAGDGAFLMTIGDLPTAVEYGANILMVVLNDGAFGQTYMQQTNLYGHTYGTTFQ
                     SPRFADIAKACGAEGVRVSDPKDVEGALRQALAATKTRPALVEVMVAERPYPKI"
     gene            complement(10575..11453)
                     /locus_tag="AC158Q_10775"
     CDS             complement(10575..11453)
                     /locus_tag="AC158Q_10775"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019474.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3340300.1"
                     /translation="MTTAYNPAAHYEIKVWDVDFRRAPGGRQLKARIYQPQGPGPFPT
                     LLDLHGGAWNDKDRLANVPMDRNLAESGILVVAVDMTIAPEAPYPASVQDANYAVRWL
                     KAKAREWKGDPAGLGVLGSSTGGHMAELLAMRPRDPRYNAHPLPAELNAAPNVDATIA
                     YVVARSPISDPHTRFQQAEKMKRKEMMEKTKRFFVPWESIFEANPQQILERGEKVSMP
                     PMFILQGELDDNVIPPIQQKFAATYRKAGGECQLEIFPGCGHMWVADPGPQTDRAHEM
                     IRAFIARQLGALRQAA"
     gene            11578..>11844
                     /locus_tag="AC158Q_10780"
     CDS             11578..>11844
                     /locus_tag="AC158Q_10780"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3340301.1"
                     /translation="MRLKGVCGALIVLAAAWSVPMPVGAQNYPQRPVRMLVGLPAGGS
                     TDIMARIVSAKLTERLGQQVVVDNRAGASGTIAIQLAVNSQPDGY"
BASE COUNT         2001 a         3696 c         4073 g         2074 t
ORIGIN      
        1 aggcacaagg caaaagtagt tgttgtctcg ctttcccctt ttcctttcta cttgtgacat
       61 gcgcctttcc gggttccagt gaggggtgac gagtgactca cgaccagcga cttgcgacgg
      121 ttcttgcggc cccggcggtt caattcagca gtcccagcgt ttcgaacttc aagtacagct
      181 cgcggcaggc ccaggcgtcg gtggcggcgt agttgatctg cgcgggcgtg aggcgcggcg
      241 ccgcccagtt cgacgtgcgg ttgcccttgg ggatgcggaa ctcgagcagc atcccggcga
      301 gattgcgcac gccggtctgg ccgaggttgc ggcggcgcgc gacgacgccc aggtcgacca
      361 cgttctcgac cgtgaacgga aacaccagct tcagctggcg caggtcgtag gcgagcccga
      421 cgccggtctt gacgatctgg ggcttcgcca gcagctcgac cagcgcggga aaaacgtcca
      481 ggcggttcaa ctggaacagg tacaccgcct gcccggtggc cgcctggacc aggcagggca
      541 ggtgaacttc gccctttctg aacgacggcc gcgtctccgt gtcgagaccg acgacgcgct
      601 cctgccggat gtcttcccgt gcctgctcca gttcccgcgg cgtttccacc agcgacaccc
      661 tgccctcgta gcggcggatg ggcaggttgt tgaggtcctc ttttgaaatg ctccgcattg
      721 agtgatgagc gacgagtgat gaaaatttcc cggaggatgc cgggaggtca tccttcgatc
      781 tttcctgcta ggatgctact ccacattcat gcacggcagg aaaggaaacg cagcatggcg
      841 atttcgggca ggtggatttt caccgcatcg atggatgtcg atgcggacaa ggaggcgctg
      901 ttcaacgagg tctacgacac ggagcacgtg ccgcacctgc gcaaggtgcc gggcgtggtg
      961 tcggtgacgc gcgcgaaact cgacacgctg cgcgtcacga tgggcggaga gacccggacc
     1021 gtcgacacgc agggcaagcc gcgctatgcc tgctacgtcg agctcgacag ccccgacgtg
     1081 ctgaccggcc cggcctggtc gaaggccgtg gacgcgggcc gctggccggg cgaggtgcgg
     1141 cccttcacgc gcaaccggca gcacaccttg cacaaagtga tgaagtgaca gctgacaagc
     1201 tgtagctttg ccgccaaggg cgccaggatg acttacgacg agtcgccaag gacgcggagg
     1261 acacgaagga agaacgtact tcgggtcgcg aaggacgcaa aggccgcgaa gaaaatatat
     1321 attggaagca aggacgcgaa gacaacgcca aggtagtgat tggatacgcc atcggaatcc
     1381 ggaattccag ataggcctta cggcggcgat atgaccaagg actcccttgt cggccacgct
     1441 tggcgcggtt ccttggcgcc cttcaggttt tgctgttgtc cttattatta tttccttcgc
     1501 ggtaaagctt tggcttttcc tttgcgttct tcgcgtaacc ctcaaggggt attgaaaaga
     1561 atccttggcg acccgtcgtg tgaggctttt tgtggtcgtt ggttgccact ctttctggct
     1621 gatccatgtt gaaagcgctg aaatccctgt tcgatgacgc cgggtcacgc ccgttgccgg
     1681 ggcacgagcg gcggcatctc cacgtggccg tggcgagcct gctgcacgaa gcgagccgcg
     1741 tggacctgcg cgaagacggg acggaacacg ccgcggcgca ggcggggctt gccgacctgt
     1801 tcgggctcac ggagcaggag gccgccgccg tgctggccga ggggcgcggg aaggcgcggc
     1861 agatgacgtc ctacttcggc gccgtggcgg tggtgaagcg cgacatgggc cagccggagc
     1921 gggtgcggct gatcgagcac ctgtggcgca tcgcctacgc cgacggcccg ctcgatccgt
     1981 acgaggatca ttacgtgcgc aagatcgggc acctgctgta cgtcaccaat accgacatcg
     2041 tgctcgcgcg aaaccgcgcg cggacggggg cgtgacaggc tgcggacccg cgcgtcgtga
     2101 ttccgttacg gcttcacgcc cgcgcactgc cgctggtatt ctctgcaatc catccaggag
     2161 gagaaaacca tgcccggccg gaacatcaag gtccgctccc gcgaaggcgg tgaattcgat
     2221 tgctacctgg ccctgcccgg cgcatccggc aaggccccgg ccgtcgtgct cgcgtccgcc
     2281 gtgcacggcg tcgacgcgga cgtgcgcgcg atcgccgacg agttcgccgg gcacggctac
     2341 atcgcggctg ccccggacct gttctggcgc tcgatcccgg gcccgctcgc ccgcgacgac
     2401 gaccgcacga agctgcgctc gcagccgcgc ctcgagaaga taagggccgg cgaggccgac
     2461 atggccgaca cgctggccga agtgcgcaag cttccgcagt tcaacgggcg ggccgcatcg
     2521 atgggtttct gctacggcgg cccctacgcg atcctcgggc ccaagcggct cggctacgac
     2581 gccggcattt cctgccacgg cacgcaattt ctcgattttc tccacgagct cgacggcgtt
     2641 acccggcccg tatgcattat ctggggcgac caggacaacc aggcgcccgc cccggtgctg
     2701 gaggcgtacc ggaaagtgcc ggcgcgcatg aacaacgtcc aggtgcacgt cgtccccggg
     2761 atactgcatg gctacatgtt tcccgggaac aggcaggcgt tcagccagag cacgcgcgac
     2821 ttttccataa agcacgcatt ggcgctactg ggcgcattgc gctgagaagc ggtgaccagt
     2881 aactggtgac cggcacccgg tgatgcaatg aggtgaaccg ggtcgaatca caaatcacga
     2941 atcacgattc acgaatcgcg ttttttgatg cgcacgccac ccctgtcgct cgcgttcgcc
     3001 gtctggggcc tgggcgcggc gctgtacctc gtggggtttt tccaccgcgt cgcgccggcg
     3061 gtcattaccc gcgagctcgc ggcggagttc gagctgaccg ccgcggcgct cggcaacctc
     3121 tcggcgttct acttctacag ctacgtcatc ctgcaggtcc cgaccggcat gctggtggac
     3181 cgctacgggc cgcggcgcat cctcacgctg ggggcggtcg gagccgcggc gggcgcgctg
     3241 ctgttcgccg gcgcgcactc gttcgcgctg gtcggggcgg ggcgcctgct gatcggcgcg
     3301 tcggtcggcg tggcgttcgt ggcgatgatg aagctctcga cgcactggtt ccatccgtcg
     3361 cgcttcgcgc tggtggcggg actcgcgctg ggcacgggca tggtcggcgc ggccagtgcc
     3421 ggcgcgccgc tgcgctggct ggcggacgta ttcggctggc gcggcgtgat cctcgccacg
     3481 gccgccgcga ccgccgcgct cgccgccgcc atctggctgg tggtgcgcga cgatccgcag
     3541 gagcgcggct acgcgagcta tctgccggcc gcgcaccacg gcgagccggc tcagtcgatg
     3601 acgggcgggc tcgcgcgcgt gctgggcagt cgcaacgtgc tgctcgtgtg cgtcatgaac
     3661 ggcggggtat cgggcctggt gctgaccttc ggcgggttat ggggcgtgcc gtttctcgcc
     3721 acccactacg gcctgtcgac cgcggccgcg gcggcgctgt gctcgctgtt gctgctggcg
     3781 tggggggtcg gcgggccgct gctcggcgcg ctgtccgacc ggctgacgga gcgcaagcgt
     3841 ctctacggcc tgggcgcggg cgttgcgacg atctgctggt gcgtgatact gctcgttccg
     3901 gggctgccgc tggcgctgct cgccgcgctg ctcgcggcgg ccggccttgc ctccggggtc
     3961 atcatgatcg gctatgccta tgccaaggag tcggcgcccg cggcgcttgc cggcacgacc
     4021 ggcggcgtca tcaacaccgg caacatgctc ggcgccatgc tgatgcagcc ggcggtgggc
     4081 tgggtgctcg accgcgcgtg ggacggcggc ctcgaggcgg gactgcgcgt ctattcgttc
     4141 gcggcctacc gcctcgggtt cgcggtactg ctggccttgc tgctggcggc cgtgttcgtg
     4201 accgcgctca cgcgcgagac gcactgccgg caacagcagt gaccggtagg gcgggccagc
     4261 ccgcgggatg agggcggagg gatgaggggt ggggtgaaat caaggcaaaa ggcacaaggc
     4321 acaaggcaca agggctcgaa acccgatgcc ggagcggcgg cgaagcgcag gccgcgccgt
     4381 taccaccact cggtttacgt aatccttctt tcgcgggacg tgctgtacga gcgcaagttc
     4441 gtgaagtgca acccggatta cgacccggag aagccgtgcg tgtacgtcgg catgacgggg
     4501 caaagccccg acgtgcgttt cgacaagcac aaggcggggc tcaaatccaa ccgctacgtc
     4561 aggaaatacg gtatccggct gatgccggaa ctgtacgagt gctacaaccc gatgccgtac
     4621 ggcgctgcgc tcgacatgga gatcgagctt gccatccacc tgcgcgagag ggggtacggt
     4681 gtgtggcaag ggtgaggggg caagtcgaaa gtcgaaagtc ggaaggcgaa aggcgaaagg
     4741 cggaagggcc tggaacccgg tgatgaggga taggggggac ttgggccgct gcgcgggaaa
     4801 ccatccgcca tccacgctgt tcaaaccagg tcgagaagcg agtaacgaga aacgagcaac
     4861 gagaaacgag caacgagaaa cgggccgccc cttgttagcc gaggcggatc tatttgaaac
     4921 aatggcagat tctggatcac tgtcaggttc atgtaagcca ccgctgatag aaaccgtgat
     4981 gagtgccgag tgtcgaggaa tgagtgtcga gtgaacaaag caaagcaggg cctttccccc
     5041 tcgacacccg acacccgacc ctcgacaccc gattcacgat taacgaacaa acaggagttt
     5101 ccgcttgaag atttcgcggc gcgcatttca atggctgttg gcgttgtgct ttgtttcggc
     5161 cgccggcgct gccgccgtgc tggcccagga tgcccaggac accccgaaac cgtttgtgcc
     5221 gttcgagggc caggagggca aggacgtggt gtgggtgccg acgcccgggg tcctggtcga
     5281 gaagatgctg gacctggcga aggtgacgcc gcaggactac gtgatggatc tcggctcggg
     5341 cgacgggcgc aacatcatcg ccgccgcccg gcgcggggcg cgcgcgctcg gcgtcgagta
     5401 caacccggac atggtcgagc tgtcgcggcg cgaggcggag aagcagggcg tcgcggacaa
     5461 ggcccagttc gtgcagggcg acatgtacga ggccgatatt tcgcaggcca cggtcatggc
     5521 gctgttcctg ttgcccgaca atctcgagaa gctcacgccg aagttcctcg acctgaaacc
     5581 gggctcgcgc atggtgttga acggcttcgc ggtgcccggg tgggaggcgg acgtgacgga
     5641 gcgggcagag ggggattgcg ggagctggtg cacggcgatg ctgtacatcg tgccggccag
     5701 ggtggcgggc acctggcggt tgccgcaagg cgagctgcgg ctcgagcaaa aattccagat
     5761 gctcaccggg acgctgacgc gcggcggcaa tcgcactccg atcaaggacg ggcggctgcg
     5821 cggagaggag atcagcttca tggccggcgg cacgaaatac gtgggccgcg tcaagggcga
     5881 cgccatcagc ggcgcgaacg gcacgtggac cgccacgcgg attcgttcca agctgcagtg
     5941 accagcgact cgtgaccaga actcccgccg agcgggagac ccatcactca tcactcgtca
     6001 cccatcccag tgatacgcct ggtgctggac accaacgtct ggctggactg gctgctcttc
     6061 ggcgacccgg acgtcgcgcc gatgaaggca gcggtcgcgc agggcagggc cgggatcttc
     6121 atggacgaag cgggcgaggc ggagctggcg cgcgtgctcg cctatccgtt tcagaacaga
     6181 acgctggacg cggcggcgca ggcggagctg atcgcgctat gcggacgcat tgcgcggaag
     6241 gcggaagttg gaagtcgaaa ggcgaatgaa agttggcttc cgaagtgcga ggatgccgac
     6301 gatcagaagt ttctcgaact ggcgttgact tgcggcgcgg catttctcgt cacccgggac
     6361 cgggccctgc tcggactggc ccggcataag ccgcggccgc tgccctatcg catcgtcaca
     6421 cccgcacagc tggccgctgc atttcgaagt gatgaggtga ttaagtgacg aagtgacgaa
     6481 aacccgcatt gccttacttc ttcacgtcat caccctgtca ccgcgtcact gcatcacttt
     6541 ctcacctggc ctgtaaaccc ttcggacacc cgggcgttga gggtgtttat gggttacgtg
     6601 cgctacagca cagtcaattc gacaatgagt gccgtatcct gaggttagct ttggaacctc
     6661 gacggaacct gaactgcagc ccggactggc gcgcgagtga cgcaactcat tgcacgaaat
     6721 agtgtcaaac gaaaagatag aaaaggcgat catcaccagg aggcgcgaca tggcgaattt
     6781 cgatagcgct gccggcgaat ccggcgacct gttcggcgag aagcctgtca cccggcgcgg
     6841 gccggcgatc aagcgcaggc cggcgcccga gctcgccgtc gcacgcgcgc tctgccggca
     6901 gctcttgcgc aagcaggcga gcccggaaaa aaagcggatc gcacacctga tctgcctgaa
     6961 cctcgtgtcg atgctgaaga acggatcgca gcggccctag cggacccgac caccgccgcc
     7021 atttaagcgt tgcggtgttt tctgctagac tggagcctcc ttaatttcga aaggaggtga
     7081 tcccgtgaga gaccttgaag gcagcaccga cttgcacggc tcgaacatag gatccacccg
     7141 caacgtaagg atgttcgacc ggcgctgagc cggcgtgcct ttgttgtaaa aacgccccgc
     7201 ggacgtagtc ccggggcgtt ttccgttttg gcggccgcgc ctgaagtcga aagtcggaag
     7261 tcggaacggg cctgaccttc gtccctcgtc accggtttat tgttaacatc cggcgcatgg
     7321 acatgcaaaa gccggccatg gatttctcgg gtgtcgggta tgacgaggcg cttgcgcgcg
     7381 cgcgggagct gatcccgttc ctgcgcgagc aggcgccgaa gtgcgaggag gcgcgcaggc
     7441 tcacgcccga ggtcatggac cggctgactc gcgccggatt gttccgctac ctgcagccga
     7501 aagtccgcgg cggcatggaa ctgccgttcg tatcctattt cgacataccc gagatactcg
     7561 cgcgcgggga catttcggtg gcctggacgg tggacaatct cgcctcccac catcgcaaca
     7621 tcacctggtt cgacccgcgg gcacaggaag aagtgtgggg cgccaacccg gacgcgggca
     7681 tcgcctccgg catcgcctac ccgcagggcc gcggtgtgcc cgcggacgac ggcatcacgc
     7741 tctccgggga atggaatttt tcctccggca ccgatcattc cgactggagc ctgctcgcct
     7801 gcatcgtgcg cgacggcgac aagatcctcg attaccggtt ctgcctgctt caccgctccc
     7861 agtacgaaat cgtggatgac tggcacacgc tcggcatgcg cgcgaccagc agcaagaccg
     7921 tgcgctgcag ggatgtgttc gtgccggctt atcgcagcgt gtccatgtgg gtcgcgcgcg
     7981 agggccactc gtggccggga ctcgccgtgc acgggaaccc gcactaccgg gtgccgatct
     8041 cggcgctcgg cggccacggc ctcggcgcct gcatgatcgg caacgcgcgc gcggcgctgg
     8101 aactggcggt cgagctggtc aaatcgcgca gcaccaatta caccggcgcg aagatgcgcg
     8161 atttccagac ggtgcaactg cgcatcggcg cggcgggggt gaaagccgac gcagcgtaca
     8221 cgctgctgag gaacgattgc ctggaagcgc agcgcgtgta cgaggagggc ggcaacctgg
     8281 atatcgagac gcggctgcgc tacaagcgaa acgccgcgca ggcggcccgc tggtgcgtcg
     8341 aggccgtgga ttcgctgcac gagatggccg gcgccaatgg catctacgac cattacccct
     8401 tgcagcgcgt attccgcgac atgcgcgccg ccgccgggca cttcagtttc agcaccgccg
     8461 cgcagctgcc gccgtggggg ctggttgcga tgggcgggga atacaagagc cccacgctct
     8521 gagggatgag ggatgaggga tgagggatga gggatgaggg atgagggatg agggatgagg
     8581 gatgaggcga actgggtccc tggctcattt attcctgagt gccgcccctt atttcgttct
     8641 tcatttttcc gacttcatcc ttaaaacccg tcactgggtc caagctcctc atccctccgc
     8701 cctcatccct catccctccc ttagatcttg ggatagggcc gttccgccac cattacttcc
     8761 accagcgccg gccgtgtctt cgtcgcggcg agcgcctgcc ggagcgcgcc ctcgacgtct
     8821 ttcgggtcgc tgacccggac gccttcggcg ccgcaggcct tcgcgatatc ggcgaaacgg
     8881 gggctttgga acgtggtgcc gtaggtgtgg ccgtagaggt tggtctgctg catgtaggtc
     8941 tggccgaagg cgccgtcgtt cagcacgacc atgagaatgt tcgcgccgta ctccacggcg
     9001 gtgggcagat cgccgatggt catgaggaag gcgccgtcgc cggccagcgc caccacgtcg
     9061 cggcccggga attccatctt ggcgacgatc gccgtcggca ggccgtagct catggcgttc
     9121 cagacgccgg actgcatgaa cgattcgggc gtcgcgatgc ggcggtaagt gcgcgcccac
     9181 atctggcagt tgccgacgtc gctcgcgacc acggcatcgt cgccgagcac ggtgttcatc
     9241 gcgtccatca gcacgccggg atagatggga ttgtcatcgc ggtgcggctc gccctgggcc
     9301 gcgatgctcg cccggacggc ggccctggcc tcggccatct gccggatgag cgcctcgttg
     9361 cgcggccgct ggtagcttcc caggcgttcg agcagcgccg tgaggaaaag accgggatcg
     9421 gccacgcgct ggtccgctcc ctggtagcgg gcgttcgcgg cgtcgtcgaa gccgacgagg
     9481 atgagggcgc gctcgggcgc tttctcgcgc agctccgcca tctcggccgt gcccgcgcgc
     9541 aggccgacgg cgaggacgag atcggtgcgc tcaagcgctg cgacgcagag gggatggctg
     9601 cggtagtgct ggaagtggcc cgcgaagaaa ggatgggtct cgggaatcac gccgatcgcg
     9661 tcctgcgcga agaccaccgg ggcctggagc ttcatcgaga gctcggcgag ctcgcgcaac
     9721 gcgcccttgc ggatgacgcc cttgcccgcc gctatgacgg gcgcccgcga ttccatgaga
     9781 cgtttcgcga agcgatccac gtctttcggg tcgggtttgg cgaccacggc gggcgccggc
     9841 ttgtaggcgg gaaggacggc gcgctcctcc tggaggatgt gctcgctgta atccgacagg
     9901 cgcggcaatt cgacgtgcac cggcccgggg cgtccgctgc gggcgacgtg gaacgccttc
     9961 gccatgacgg ccgggatgtg ctcgatactc tcgacgcgcg cgctccactt ggtgatcttc
    10021 ttgaacatct cgtggacgaa ggcgggatcg tccactccgt gaaaggcctc gaggtcggcc
    10081 ttcagcggca ccgcgccgcc gatatggacc atcggggagg ccgcgccgta cgcctgcgcc
    10141 acgccggcca tggaattcag gccgcccggc ccggcagtga cgacgcaaac gccgggcttg
    10201 ccggtcaggc ggccgtaggc gtccgccatc agcgagacgt tcggctcctg cttgcaggtg
    10261 acgaagcgga tggattttac ccgcgacagc ccgtcgtaaa tcgggtggat gtgggagccg
    10321 gggacgctgt agacgcgctc gacgccttcc tcgcgcagcg cctcggcgac ggcttcgccg
    10381 gcgtgcatgg gcgcgcgctg cgaggtcata tcagtgacgg gtgacgggtg acgagggtcg
    10441 ggtgaagagt gacgagggta gggtgaagtg ggtcgtgggt cgggtgtcga gggtcgaggg
    10501 gggtaaaacc ctgttgcgtt ttttcactcg acactcaatc ctcgacactc ctttccctca
    10561 ttcctcgggc ggcgctacgc cgcctggcgc agcgcgccca gctgccgcgc gatgaaggcg
    10621 cggatcatct cgtgggcgcg gtcggtctgc gggcccgggt cggccaccca catgtgcccg
    10681 cagccgggaa agatctcgag ctggcactcg ccgccggcct tgcggtaggt cgcggcgaac
    10741 ttctgctgga tcggcgggat cacgttgtcg tcgagctcgc cttgcaggat gaacatcggc
    10801 ggcatcgaga ctttttcgcc gcgctcgagt atctgctgcg ggttggcttc gaagatggat
    10861 tcccacggca cgaagaatct cttcgtcttt tccatcatct ccttgcgctt catcttctcg
    10921 gcctgctgga agcgggtatg cggatcgctg atcggcgagc gcgccacgac gtacgcgatg
    10981 gtcgcgtcca cgttcggcgc cgcattcaat tctgcgggga gggggtgcgc gttgtagcgc
    11041 gggtcgcgcg ggcgcatggc gaggagttcc gccatgtggc cgccggtgga gctgcccagc
    11101 acgccgagcc cggccggatc gcctttccac tcgcgcgcct tcgccttcaa ccagcgcacc
    11161 gcatagttcg cgtcctggac cgaggcggga tagggcgcct cgggcgcgat cgtcatgtcg
    11221 accgcgacca ccaggatgcc gctttccgcc aggttcctgt ccatcggcac gttggccagg
    11281 cggtccttgt cgttccatgc gccgccgtgc aggtcgagca gcgtcgggaa cggccccggg
    11341 ccctgcggct ggtagatgcg ggccttcagc tgccgcccgc ccggcgcgcg ccggaagtcc
    11401 acatcccaga ccttgatttc gtagtgagcc gcggggttgt aagccgtcgt catcgtgatc
    11461 ctttcgtcta tcgggtggtg ctgcgtggag cgggaaatct tactctatac tgagcggcaa
    11521 gtgacgagtg atagacgaag aatgatggtc cgctcaagga tggatacagg aggagggatg
    11581 cgattgaaag gggtttgcgg cgcgctgatc gtgctggcgg ctgcgtggag cgtgccgatg
    11641 ccggtcgggg cacagaatta tccccagcgc ccggtccgca tgttggtcgg gctcccggcc
    11701 ggcggcagca ccgacatcat ggcgcggatc gtgtcggcca agctgaccga gcgcctcggc
    11761 cagcaggtcg tggtggacaa tcgcgcgggc gcgagcggca ccatcgccat ccagctcgcc
    11821 gtcaactccc agccggacgg ctac
//
LOCUS       DBNGSG010000413         2695 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4378908, whole genome shotgun sequence.
ACCESSION   DBNGSG010000413 DBNGSG010000000
VERSION     DBNGSG010000413.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4378908"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..1872)
                     /locus_tag="AC158Q_10785"
     CDS             complement(103..1872)
                     /locus_tag="AC158Q_10785"
                     /inference="COORDINATES: protein motif:HMM:NF013188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HIR3340302.1"
                     /translation="MNEEQFLVQATVFLAAAVIGVPLLKRLGLSSVLGYIVAGAAIGP
                     WGLALAGDVESVRAFSEIGIVLLLFLIGLELLPSRLWELRRSVFGLGAAQVAATGAVL
                     GLGAWALGVTPGAAAIIGFGLALSSTAVGLQILIERKQLTTPQGRAGFAVLLFQDLAV
                     IPLLAVLPALADRSAGVVHSVGGFSPLVTLLLFASVVVAGRYLLRPYFRVLSTAGSQE
                     VFTAGALLVVLAVGVFMHKLGISMALGAFVAGMLLADSEFRHEIEADIEPFKGLLLGL
                     FFMAVGMSVNFGLIGAEPLQVAGLVIALVAVKAAIVFALARAFGEEAPSAYAVAVVLS
                     QGGEFAFVLFSLAAGEKLLPAGLADLLVAVVTISMLTTPFLVNLLHCLPQSKEPAVAE
                     GASPTGKENPVIIAGFGRFGQIVGRIVATRGIPFTALDSDSEQLDFVTQFGEKAYFGD
                     ASRLDLLRAAGADKASVLVIAIDDIEASMKCAAIVKQHFPRIKIFARARNRLHAYQLL
                     DLGITRVWRETFHASADMAGRVLTELGLPDSTAEETVRIFTLHDERLLRRAAAHHADR
                     EKLIEIARAGRAELRSLFEQDRG"
     gene            complement(2095..>2695)
                     /locus_tag="AC158Q_10790"
     CDS             complement(2095..>2695)
                     /locus_tag="AC158Q_10790"
                     /inference="COORDINATES: protein motif:HMM:NF018595.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HIR3340303.1"
                     /translation="CIHARECVHGLPAVFDPGRKPWVDPKAAPADEVARVIERCPTGA
                     LQYERKDGGPQESPPPMNTIRIAADGPLYLHGRVRVDGADGQCVVRDTRVALCRCGKS
                     GLKPFCDGSHTKAGFKDAGAVPRPEEVETEPGKSEEGALVVTCTADGPLAVTGGFELM
                     DAQGAVVARGNVAWLCRCGGSANKPFCDGTHKKNGFKAP"
BASE COUNT          484 a          953 c          867 g          391 t
ORIGIN      
        1 tatcgttgat ccccctggta tgaaaaatga agatgggccc tcagaggggc ctgattatgc
       61 acgatcccaa ctgattttgc cgccggcgca ccggcggcaa aatcatcccc ggtcctgctc
      121 gaacagcgag cgcagctcgg cgcggcccgc ccgggcgatc tcgatcagct tctcccggtc
      181 ggcgtggtgc gccgcggcgc gccgcagcag gcgctcgtcg tgcagggtga agatgcgcac
      241 cgtctcctcc gccgtcgaat ccggaagccc cagctcggtc agcacccttc ccgccatgtc
      301 ggcgctcgcg tggaacgtct cccgccacac gcgcgtgatg ccgaggtcga gcagctggta
      361 ggcgtgcagg cgattgcgcg cgcgcgcgaa gatcttgatg cgcggaaaat gctgcttcac
      421 gatggccgcg catttcatgg acgcctcgat gtcatcgatc gcgatcacca ggacggaggc
      481 cttgtcggcc ccggccgcgc gcagcaggtc gagacgcgag gcgtcgccga aataggcctt
      541 ctcgccgaac tgggtcacga aatcgagctg ctccgagtcg ctgtcgagcg cggtgaacgg
      601 gatgccgcgc gtcgcgacga tgcgcccgac gatctgtccg aagcggccga agccggcgat
      661 gatcaccggg ttttccttgc ccgtgggcga agcgccttcg gcaacagcgg gctccttgct
      721 ctgcggcagg cagtgcagca ggttcaccag gaagggcgtg gtcagcatgc tgatcgtgac
      781 caccgccacc agcaggtcgg ccagcccggc ggggagcagc ttttcgccgg ccgccagcga
      841 aaacagcacg aacgcgaact cgccgccctg cgacagtacc accgcgacgg cgtaagccga
      901 cggcgcctcc tcgccgaaag cccgcgcgag cgcgaagacg atggccgcct tgaccgcgac
      961 cagcgcgatc acaagtcccg cgacctggag cggctcggcg ccgatcagcc cgaaattcac
     1021 cgacatgccc accgccatga agaagaggcc gagcagcagt cccttgaacg gctcgatgtc
     1081 ggcctcgatc tcgtgccgga actcggagtc ggcgagcagc atgcccgcga cgaacgcccc
     1141 gagtgccatc gagatgccga gcttgtgcat gaagaccccg accgccagca cgacgagcag
     1201 cgcgccggcc gtgaacacct cctggctccc cgccgtcgag agcacccgga agtacggcct
     1261 cagcaggtag cgcccggcca cgaccaccga ggcgaacagc agcagcgtca ccagcggaga
     1321 gaaaccgccc acggaatgca cgactccggc ggatcggtcg gccagtgccg gcagtaccgc
     1381 gagcaacggg atcacggcca ggtcctggaa caggagcacc gcgaatcccg cccgaccctg
     1441 cggcgtcgtg agctgcttgc gctcgatcag gatctgcagt ccgacggcgg tcgaggacag
     1501 cgccagtccg aacccgatga tggccgccgc gcccggcgta acgcccagcg cccacgcccc
     1561 caggccgagc acggcgccgg tcgcggccac ctgcgcggcg ccgagcccga atacggagcg
     1621 gcgcagctcc cacagccgcg acggcaggag ctccagcccg atcagaaaca gcagcagcac
     1681 gatgccgatc tcggagaacg cgcgcacgct ctcgacgtcg ccggcaagcg cgagccccca
     1741 cggcccgatc gccgcgccgg ccacgatgta gcccagcacc gaggagaggc cgagccgctt
     1801 cagcagcggc acaccgatga ccgccgcggc gagaaacacc gttgcctgga cgaggaattg
     1861 ttcttcgttc atggaagacc tttgaattga ccagaagtca tctcaaaaac taatttagcc
     1921 acggaaacac acggaaccac acggaaattc ttggatgatt atgaagactc taaaggggtc
     1981 gggttattgt ccgtgtcatt ccgtgtgatt ccgtggcaat ttttgagacg ggttctaatc
     2041 actacatgaa ctcccctcct gatcaaggag gggtggcccg cggggccggg gtggtcaggg
     2101 cgccttgaac ccgttcttct tgtgcgtgcc gtcgcagaaa ggcttgttgg cggagccgcc
     2161 gcaccggcac agccatgcga cgttgccgcg ggcgaccacg gcgccttgcg cgtccatcaa
     2221 ctcgaagccc ccggtcacgg cgagcggccc gtcggccgtg caagtgacga cgagtgcgcc
     2281 ctcttccgac ttgcctggtt cggtctcgac ttcctccggc ctaggcaccg cgccggcgtc
     2341 cttgaacccg gccttggtgt ggctgccgtc gcaaaacggc ttcaagccgg atttcccgca
     2401 gcggcacagc gccacgcgcg tgtcccgcac gacgcactgc ccgtccgccc cgtccacccg
     2461 cacccggccg tgcaggtaga gcgggccgtc ggccgcgatc cttatcgtgt tcatcggcgg
     2521 cggggactcc tgcggcccgc cgtccttgcg ctcgtactgg agcgcgcccg tcgggcagcg
     2581 ctcgatcacg cgcgcaacct cgtccgccgg cgccgccttg gggtccaccc agggcttgcg
     2641 gccgggatcg aacaccgccg gcagcccgtg aacgcattcc cgggcgtgaa tgcag
//
LOCUS       DBNGSG010000414         5053 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2002338, whole genome shotgun sequence.
ACCESSION   DBNGSG010000414 DBNGSG010000000
VERSION     DBNGSG010000414.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5053)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5053)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5053
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2002338"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..284
                     /locus_tag="AC158Q_10795"
                     /pseudo
     CDS             1..284
                     /locus_tag="AC158Q_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452612.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="nitrite reductase (NAD(P)H)"
     gene            309..692
                     /gene="nirD"
                     /locus_tag="AC158Q_10800"
     CDS             309..692
                     /gene="nirD"
                     /locus_tag="AC158Q_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HIR3340304.1"
                     /translation="MKDELRSARAPDTHYSTLAADGGWIAVCRLADIVPNTGVCALVA
                     GRQVAVFRLDDDSVYALSNHDPFSRANVLSRGIVGDLKGELVVASPVYKQHFSLASGQ
                     CLEDPDVRIPVFAVRLEGDTVMVEA"
     gene            complement(793..2775)
                     /locus_tag="AC158Q_10805"
     CDS             complement(793..2775)
                     /locus_tag="AC158Q_10805"
                     /inference="COORDINATES: protein motif:HMM:NF040438.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HIR3340305.1"
                     /translation="MKGACIARAALCALVFAAPPSIARLTEINVAAVEPFAGGHAFGD
                     AGAYERVRGTFKGELDPADPRNKVIVNLDKAPRNAAGRVEYEADFYILRPVDAARGSR
                     KILYDVTNRGRQYAHWMFGDARTVRNDPRGLEDAGNGLILRRGYTIVWSGWDPDAPRT
                     NNGLAMKAIIATDGGAPIVRVIRDELLSGGRGPQRKTFRLTHEAAMLDPARARLTVRR
                     NETDPRQEIPAGGWAYVNSREIRLLPEGTLPEPGSLYELHYPATNPRVLGIGLAATRD
                     LVSFLRYETADSQGNANPAGAGIRHALAFGRSQSGRYLRDHVRDGFNQDESARKVFDG
                     VMAHTAGVGGVFLNAEFGQPARTNTQHEDHAFPENAFPFSTARMTDPVTGKAGGLLRN
                     DGFDPLWMETNTSTEYWQKGASLLVTDPLGTRDVPHPANARGYLIAGTQHGGQAWMTS
                     TAGPCANPRNPHSPTPAARALLVALDEWVSEGKAPPASRTPRLKDRTLVMPDKIAFPA
                     IPGVAVARRMSEFGVLKDWIKPEMDMTKPYRPLVTQVDADGNETSGILLPDIAVPLGT
                     HTGWNLYRKPFAEGELCDRFGTYIPFAATRAERETNNDPRPSLEERYGGHAGYVRRVE
                     QAVKKLVTERLLLAEDGELFVAKAKNEENAKRFTQR"
     gene            complement(2838..3845)
                     /locus_tag="AC158Q_10810"
     CDS             complement(2838..3845)
                     /locus_tag="AC158Q_10810"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3340306.1"
                     /translation="MPKKCRRPSLSWFAGALAIALLAAPAAALAQAAWKPDRPVELIS
                     GSAAGNSVDRMLRTIAKTVQEKRFVDVPVTVLNKPGGGNSVAWSYLNQRAGDGHFLMV
                     ANLNLSAGHLTGTSTFSYRDFSAVCLLFDEYIALLVKADSPVKDGRDLIERLRKDPSS
                     LSLSFSSVAGGANHIAAGLVLKAAGVDVKKVRTVVFDSASKAVTAMLGGHVDVVSSSV
                     SVAVTQLKAGTVRVVGVSSPRRLAGVFADVPTWKEQGVDGTFSNYRGLIGPKGMAPAQ
                     VAYWEGVYSALDKDESWRADLERNLWVGHYLNGRDTRKYWDDLSVQVRAVLDDVGLLK
                     Q"
     gene            complement(3980..4780)
                     /locus_tag="AC158Q_10815"
     CDS             complement(3980..4780)
                     /locus_tag="AC158Q_10815"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HIR3340307.1"
                     /translation="MRDKTVAILESRLGRQFVELIAKHGGRPLHAPALAEVPDLDRGV
                     IAGLVTDLEASPPRAAIFQTGVGTKALFEATDSLGLTERLLALLAKTIVAVRGPKPTG
                     ALRGRGVRVDLSAKDPFTTTEVLEILARTPVAGERVVVQRYGVTNVELEEALQARGAR
                     VVEIPTYRWSLPQDTRPMVELLDAIEGRRVDAVAVTNAAQVHNLFELAATLGRADGLR
                     AGLNRTLVASIGPVSSEALKKFGVAVGLEASPPKLGPLVSALEDALSR"
BASE COUNT          801 a         1707 c         1716 g          829 t
ORIGIN      
        1 ggctggagaa cctggaaggc ggcatcgatt acgtgcgcaa ggtcgtgtgc gaggattagc
       61 tcgcgctcgg cgccgagctc gaggccgact tgcaaaggca agtcgagacc tacgcctgcg
      121 aatggaagaa ggcgatcaac gatccgcaga cgctgaagcg cttcctccac ttcgtgaaca
      181 gcgaggcgcc ggacgcgaac gtcttgttcg ttgccgagcg cggccagatc cggcccgcgc
      241 gcgccgacga gcgcgagccg gcgcttacgc tctccgaggg atgagtgtcg aggggcgagg
      301 ggtgagcaat gaaggatgaa ctacgcagcg caagagcacc cgacacccat tactcgacac
      361 tcgcggcaga cggcggctgg atcgcggtct gccgcttggc cgacatcgtg cccaacaccg
      421 gcgtgtgcgc gctcgtagcc gggcgccagg tcgcggtgtt ccgcttggac gacgacagtg
      481 tctacgcgct ttccaaccac gatccgtttt cgcgtgccaa cgtgctctca cgcggcatcg
      541 tcggcgatct caagggcgag ctggtcgtgg cctcgcccgt ctacaagcag catttcagcc
      601 tcgcctcggg ccagtgcctg gaggacccgg acgtgcgcat cccggtattt gccgtgcgcc
      661 tggaaggcga cacggtcatg gtcgaagcct gacgacggca aagccgcgtt ctagtgggtc
      721 gaatcacggc tcacgaaccc ccaccccaac cctcctcgtt gtgaaggggg aggcgcagga
      781 gggggtcacg actcaccgtt gcgtgaagcg cttcgcgttc tcctcgttct tcgctttcgc
      841 gacgaacaac tcgccatctt cggccagcag cagacgctcg gtgacgagct tcttcaccgc
      901 ctgttcgacc cggcgcacgt agccggcgtg gccgccgtag cgctcctcga gggacggccg
      961 cggatcgtta ttcgtctcgc gttcggcgcg ggtggcggca aacggaatgt aagtgccgaa
     1021 gcggtcgcac agctcgcctt ccgcgaacgg cttgcggtag agattccagc cggtatgggt
     1081 cccgagcggc accgcgatgt cggggagaag aatgccggaa gtctcgttgc catcggcgtc
     1141 cacctgcgtc accagcgggc ggtacggctt cgtcatgtcc atctcgggct tgatccagtc
     1201 cttcagcacg ccgaattcgc tcatgcgccg cgccactgcc acgcccggga tcgcgggaaa
     1261 cgcgatcttg tccggcatga ccagggtcct gtccttgagg cgcggcgtac gcgaggcggg
     1321 cggcgctttc ccctcgctta cccattcgtc gagcgcgacc agcagggcgc gcgccgccgg
     1381 cgtcgggctg tgcgggttgc gcgggttggc gcaaggcccg gcggtggagg tcatccacgc
     1441 ctgcccgcca tgctgggtgc cggcgatcag gtatccgcgc gcgttggcgg ggtgcggcac
     1501 gtcacgcgtg ccgaggggat cggtgaccag gagcgacgcg cccttctgcc agtactcggt
     1561 cgaggtgttg gtttccatcc acaacggatc gaagccgtca ttgcggagca ggccgccggc
     1621 ctttccggtc accgggtccg tcatgcgcgc cgtggagaag gggaacgcgt tctcgggaaa
     1681 ggcgtggtct tcatgctggg tattggtgcg cgccggctgg ccgaattcgg cgttgaggaa
     1741 cacgccaccc acgccggcgg tatgcgccat tacaccgtcg aacaccttgc gcgccgactc
     1801 gtcctggttg aagccgtcgc gaacgtgatc gcgcaggtag cgtccgctct gcgagcgtcc
     1861 gaacgccaat gcgtgccgga tgccggcgcc cgcgggattg gcattgccct gcgagtccgc
     1921 cgtttcgtag cgcaggaatg acaccaggtc gcgcgtggcg gcgaggccga tgccgagcac
     1981 gcggggattc gtcgcggggt agtgcaattc gtagagcgag ccgggctcgg gcagcgtgcc
     2041 ctcgggcagc aggcggatct cgcgcgaatt cacgtaggcc cagccgccgg cggggatctc
     2101 ctgccgcggg tcggtttcgt tgcgccgcac ggtaagcctc gcccgggccg ggtcgagcat
     2161 cgccgcctcg tgggtgaggc ggaaggtctt gcgctgcggg ccgcgcccgc cgctcaacaa
     2221 ctcgtcgcgg atcacgcgca cgatcggcgc accgccgtcc gtggcgatga tcgccttcat
     2281 tgcgaggccg ttgttggtgc gtggcgcatc cgggtcccag ccgctccaca cgatggtgta
     2341 gccgcggcgc aggatcagcc cgttgcccgc atcctcgagg ccgcggggat cgttgcgcac
     2401 ggtgcgagca tcgccgaaca tccagtgggc gtactgccgg ccgcggttgg tgacgtcgta
     2461 cagtatcttg cggctgccgc gcgcggcgtc caccggccgc aggatgtaga aatccgcttc
     2521 gtactcgacg cgccccgcgg cgttcctcgg cgccttgtcg agattgacga tcaccttgtt
     2581 gcgcgggtcg gcgggatcga gctcgccctt gaacgtgccg cgcacccgct cgtaggcgcc
     2641 ggcgtcgccg aaggcgtggc ccccggcgaa cggctcgacg gcagcgacgt tgatctcggt
     2701 cagccgcgcg atggacggcg gcgcggcgaa aacgagcgcg cacagcgcgg cgcgcgcgat
     2761 acaggctccc ttcatgaacg gctcctccgg tcgtgccaaa ttcaagaacg aatggacagg
     2821 attcacagga ttgacgttca ctgtttcagc aggccgacgt cgtcgagcac cgccctcacc
     2881 tggaccgaca ggtcgtccca gtacttgcgg gtgtcgcggc cgttcaggta atgccccacc
     2941 cacaggttgc gctcgaggtc cgcgcgccag ctctcatctt tgtcgagcgc cgagtagaca
     3001 ccttcccagt acgcgacctg ggccggcgcc atgcctttcg gcccgatcag gccgcgatag
     3061 ttcgagaacg tcccgtccac gccctgttcc ttccacgtcg gcacgtccgc gaacacgccg
     3121 gcgaggcggc gcggcgacga aaccccgacc acgcgcacgg tcccggcctt caattgagta
     3181 accgccactg agacgctgga agagacgacg tccacgtggc cgcccagcat ggcggtgacc
     3241 gccttgctgg cagagtcgaa caccacggtg cgcaccttct tcacgtccac cccggccgcc
     3301 ttcagcacca gccctgcggc gatgtggttc gcgccgcccg ccaccgagga gaacgagagg
     3361 ctcaacgacg aggggtcctt cctcaggcgc tcgatcaggt cgcggccgtc cttgacgggg
     3421 gagtcggctt tcaccaggag cgcgatgtac tcgtcgaaca ggaggcacac cgcgctgaag
     3481 tcgcggtagc tgaacgtgct ggtgccggtc aggtggccgg ccgacaggtt caggttggcg
     3541 accatgagga aatgcccgtc gccggcgcgc tggttgagat agctccaagc gacggaattg
     3601 ccgccgccgg gcttgttcaa cacggtgacg ggaacatcga cgaaacgttt ctcctgcacg
     3661 gtcttcgcga tggtgcgcag catgcggtcc acgctgttgc ccgccgcgga gcccgagatg
     3721 agctcgaccg gccggtcggg tttccacgcg gcctgcgcca gcgcggcggc cggcgcagcg
     3781 agcagggcga tcgcgagcgc gcccgcgaac caggaaagag aggggcggcg gcatttcttc
     3841 ggcacggttt tctcctccgg atattgatct gcggataatg ttttgttgag gagtatagcg
     3901 ccactattgc aggccaaagg caggggacgg cgcataaagc ccgttacctt gccactcgtc
     3961 actcgacacc cgacacccgt caccgcgaca gcgcgtcctc cagcgccgag accaggggcc
     4021 ccagcttggg cgggctcgct tcgagtccca ccgccacacc gaatttcttc aacgcctccg
     4081 aggacaccgg gccgatggag gcgaccagcg tgcggttcag gccggcccgc agcccatccg
     4141 cgcgcccgag cgtggccgcc agttcgaaca ggttgtggac ctgcgccgcg ttggtgacgg
     4201 cgacggcatc cacccggcgg ccctcgatcg cgtccaggag ttcgaccatc ggccgcgtgt
     4261 cctgcggcag cgaccagcgg taggtgggga tctcgaccac ccgcgcgccg cgcgcttgca
     4321 gcgcctcttc cagctcgacg ttggtgacgc cgtagcgctg cacgacgacg cgctcgcccg
     4381 cgaccggcgt ccgcgccagt atttccagca cctcggtcgt ggtgaacgga tccttcgcgc
     4441 tcaagtcgac ccgcacgccg cgcccgcgca gcgcgccggt cggcttcggt ccgcgcaccg
     4501 ccacgatggt tttcgcgagc agggcgagca gccgttcggt caggccgagc gagtcggtcg
     4561 cctcgaacag cgccttcgtg ccgacgccgg tctggaaaat cgcggccctg ggcgggctcg
     4621 cctccagatc ggtgacgagc ccggcgatga cgccgcggtc caggtccggg acctcggcca
     4681 gcgccggggc gtgcagcgga cgcccgccgt gcttcgcgat cagctcgacg aactgccggc
     4741 cgaggcggga ctccaggatc gccaccgtct tgtcgcgcat ggcgctatct taagtcgaaa
     4801 ggcgaaaggc gaaagtcgaa atgcgaaagg cgtgacaggt cgccagcttc tcatcatcct
     4861 ccagtccagt ttggcgggag gttccccgat gccgtcactc cttggtgcga accagcacgg
     4921 cgtcgacgga actcagcggc acgacggcat cgaagaattc ccggccggag acggccccga
     4981 tctgcaccac gcgggcgttg gctgcgcgca cgacgccggt gatttcctgg ccggagcgca
     5041 accgcagcgt cac
//
LOCUS       DBNGSG010000415         5829 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3869064, whole genome shotgun sequence.
ACCESSION   DBNGSG010000415 DBNGSG010000000
VERSION     DBNGSG010000415.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5829)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5829)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5829
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3869064"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="AC158Q_10820"
     CDS             complement(<1..245)
                     /locus_tag="AC158Q_10820"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3340308.1"
                     /translation="MANETTHELAGKVALVTGASRNIGRSIALALAAGGASVAVNARS
                     SKDDADKVVQEIRAAGGQAETFLADIVDPKAVGAMVEG"
     gene            415..798
                     /locus_tag="AC158Q_10825"
     CDS             415..798
                     /locus_tag="AC158Q_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 family protein"
                     /protein_id="HIR3340309.1"
                     /translation="MSEEESREKNVLGGPLRACSFAPITGYFRDGCCATHGVEGVAHL
                     VCVRVTAEFLEFSRNRGNDLSTPRPEMRFRGLQPGDRWCLHVLRWVEAWEAGMAPRVV
                     LEATHEDVLKFAPLTALKRHALDVH"
     gene            857..1420
                     /locus_tag="AC158Q_10830"
     CDS             857..1420
                     /locus_tag="AC158Q_10830"
                     /inference="COORDINATES: protein motif:HMM:NF015871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH/Skp family outer membrane protein"
                     /protein_id="HIR3340310.1"
                     /translation="MEIKATVTRHKTQESGGRNRRFYFCLVPFALCLAVAADAAAQGV
                     KVGYVNLARIERESAASQRALEVLKQEFEPRHQQVREMQQRIQAAREQFEKDRGQMPA
                     AEAQAKGREIGDMMRQSDQRVVQISQEFELRRNELRARLIAQVKAAITAVAEAGKFDL
                     VLQEAVFAGPAIDITDSVLKELAQRPQ"
     gene            complement(1544..2515)
                     /locus_tag="AC158Q_10835"
     CDS             complement(1544..2515)
                     /locus_tag="AC158Q_10835"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3340311.1"
                     /translation="MKPYYGMRLICAALVAAFSGAVFAQAYPVKPVRLVVPSSAGGGT
                     DIVARILTPEFSKRLGQQVVVDNRPGAGTMIGIDHVAKAAPDGYTLLMGLSTLAINSA
                     LQKKVPYDPVNDFAPITVAVTSASILVVHPSLPVKTLKELISFARARPGQLNYASAGA
                     GTYPHMTYELFLSMARLKMVHIPYKGTAPAMIDMLAGQVATMAATVLTGLPHIRSGRL
                     RPLGITSAKRNAVVPDIPTVAEGGLPGYEAVQWYAVLAPAKTPRNIVTKLHADLVQVL
                     NSPEIKKRFAADAAETVGNTPEEFARHIRSELDKWEKVARNAGIEKQ"
     gene            2639..3478
                     /locus_tag="AC158Q_10840"
     CDS             2639..3478
                     /locus_tag="AC158Q_10840"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3340312.1"
                     /translation="MRTQLVTIPTDTIPLEGAFHEPDGGEAAGAVLLFHGNTMNFYVG
                     APRFLPPVLTRLGLACLAFNRRGHDILSTRNSRQAEGGAFQFTREAIADNRIAAEWLA
                     ARGFPAPVVIGHSNGGMLGTQHVVDHPRTPALVLLSSGRGGARPVSGGREGLFAGDRE
                     AEITAQAKSLMEAGRGRELMFMPGWWYVISAESFLDRVKTVPDTIALAPKIKCPVLAI
                     RGDKEDPERYPAEEYGRAAGGPCRVEIIPDCDHFYNGREDHVANVVASWLAQTLRLPG
                     VKS"
     gene            complement(3765..5018)
                     /locus_tag="AC158Q_10845"
     CDS             complement(3765..5018)
                     /locus_tag="AC158Q_10845"
                     /inference="COORDINATES: protein motif:HMM:NF019912.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LVIVD repeat-containing protein"
                     /protein_id="HIR3340313.1"
                     /translation="MTADGKMQPGRQASQTLALNMKLLGHHDMGGFGGMGEGMGMQKT
                     KDGRRVIWLGHEAAPKNFTAIDVTDPRNPKMIIQTELPHGKMRSNSLEVFGDTLYVAH
                     QIRGERGMTPAGFDIWDIARPEQPRKITHFDCSGPHSLGVHCVWTGDGEYLHISSGAA
                     DFQPRDPKDHQFYRIIDVRNPSKPVEAGRWWYPGQREGDAEPPLPRHPRFDGGYRPHN
                     TQCYPQRPDRAYVSYIDGGLVILDISDKSKPKMISRYNYSPPYNGFTHTAMPLFEKGL
                     LIVSDECTKDDGEDWPKLTWVFDIRDEKNPVPISTLPLPPVEVFGKRGGRYGSHNLYE
                     NYPGPCSWRSENIVLATFFNGGVRAYDVSNPYQPREVAYFVPGAPPLSPKGAIQINDV
                     YVDDRRIVYAGDRFTGGLYILEMNI"
     gene            complement(5185..>5829)
                     /locus_tag="AC158Q_10850"
     CDS             complement(5185..>5829)
                     /locus_tag="AC158Q_10850"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HIR3340314.1"
                     /translation="DYTPLATLFSEHMTFVVRPDSPLKTGRDIQEALKRDPQSLNIAI
                     GVAIGATNHLTVALLMKSMGVDVRKLKTVVFQANAQTVTSVMGGHVDLAPLSVGAALR
                     AAAAGQLRIVGITSDRRGDGPLASIPTWKEQGFDVVFTNTRLLIGPRGMTPAQVAYWD
                     TALERVVQSEEWKSAVGKQHGVTDYLGSRQSPQRMAAIYEQLKGALVDAGLAKE"
BASE COUNT         1028 a         1778 c         1959 g         1064 t
ORIGIN      
        1 ccctcgacca tcgcgccgac ggctttcggg tcgacgatgt cggcgaggaa cgtctccgcc
       61 tgcccgccgg ccgcgcggat ttcctgcacc accttgtcgg cgtcgtcctt cgacgagcgc
      121 gcgttgacgg cgaccgaggc gccgcctgcc gcgagcgcga gcgcgatcga gcggccgatg
      181 ttgcgcgagg cgccggtgac gagggcgacc ttgccggcga gctcgtgtgt cgtttcgttt
      241 gccatatcgt ggaactccgt ttgtcggttg ggtgcggaag gatagcggat tgtgccgggc
      301 gccggttttt tcggctacca tttgctgaag gcacaaggca caaggcacaa ggcacgagga
      361 agaggcgtgg agatatgaac cggccgccgt tcgccggcca cgccacaccg gtcaatgagc
      421 gaggaagagt ccagggagaa gaacgtgctc ggcggcccgc tgcgggcgtg cagcttcgcg
      481 ccgatcaccg gctatttccg cgacgggtgc tgcgccacgc atggcgtgga aggcgtggcg
      541 cacctggtgt gcgtcagggt cacggcggag ttcctcgagt tctcgcgcaa ccgcggcaac
      601 gatctcagca cgccgcgccc cgagatgcgc ttccgcggcc tgcagcccgg ggaccgctgg
      661 tgcctgcacg tgctacgctg ggtcgaggcg tgggaggccg gcatggcgcc ccgcgtcgtg
      721 ctcgaggcga cgcacgagga cgtgctcaag ttcgcgccgc tcacggcgct gaagcggcat
      781 gcgctcgatg tgcactgacg tgcggtgacg agtgtcgagt gtcgagtgag gggtgacgag
      841 tgatgggtga ggagtgatgg aaatcaaggc aacagtcaca aggcacaaga cacaagagag
      901 cggcggcagg aacaggcgtt tctacttttg ccttgtgcct tttgccttgt gcctagccgt
      961 ggcggcggat gcggcggcgc agggcgtcaa ggtcggctat gtcaacctgg cccggatcga
     1021 gagggagtcg gccgcgtccc agcgcgcgct ggaggtattg aagcaggaat tcgagccacg
     1081 ccaccagcag gtgcgggaga tgcagcagcg gatccaggcc gcccgggagc agttcgagaa
     1141 agacaggggc cagatgcccg ccgccgaggc gcaggcgaag ggacgcgaaa tcggcgacat
     1201 gatgcggcaa tccgaccagc gggtcgtcca gatctcgcag gaattcgagt tgcgcaggaa
     1261 cgagctgcgc gcccggctca tcgcgcaagt caaggccgcg atcacggccg tcgccgaggc
     1321 gggcaaattc gacctggtgc tgcaggaagc cgtcttcgcc gggcccgcca tcgacatcac
     1381 ggacagcgtg ctgaaggaat tggcgcagcg gccgcagtaa gggatgaggg atgagggatg
     1441 agggcggagg gatgagggaa agtgtcgagg attgagtgtc gagtgaaaaa acgtaatggt
     1501 ggtttacttc cctcgaccct cgaccctcgt cacccgtcac tcatcactgc ttttcaatgc
     1561 ccgcattgcg ggcgaccttc tcccacttgt cgagctcgct gcgaatgtgg cgcgcgaact
     1621 cttccggcgt gttgccgacc gtctcggcgg cgtcggcggc gaagcgcttc ttgatttccg
     1681 gcgagttcag gacctggacc agatccgcgt gcagtttggt aacgatgttc ctgggcgttt
     1741 tcgcaggcgc cagcacggcg taccactgca cggcttcgta gccgggaagc ccgccttccg
     1801 caaccgtcgg aatatcggga actacggcgt tgcgcttcgc gctggtgatg ccgagcgggc
     1861 gcaggcggcc ggaccggata tgcggcagcc cggtcaggac ggtggccgcc atggtcgcga
     1921 cctggccggc cagcatgtcg atcatggcgg gggcggtgcc cttgtagggg atgtgcacca
     1981 tcttcagcct ggccatcgac aggaacagct cgtaggtcat gtgcgggtag gtgccggcac
     2041 ccgccgacgc gtagttcagc tggccgggac gggcgcgcgc gaacgaaatc agctccttca
     2101 gcgtcttcac cggcagcgag ggatgcacga ccaggatgct ggccgacgtc accgctaccg
     2161 tgatcggcgc gaaatcgttg accgggtcgt acggcacctt tttctggagc gccgagttga
     2221 tcgccagcgt ggacagcccc atcagcaggg tgtagccatc gggggccgcc ttcgccacgt
     2281 ggtcgatgcc gatcatcgtc ccggcgccgg ggcggttgtc gaccaccacc tgctggccca
     2341 ggcgcttcga gaattcgggc gtgaggatgc gggcgacgat atcggtgccg ccgccggccg
     2401 acgacggcac taccagccgc accggcttga cgggataggc ttgcgcgaaa acagcgccgg
     2461 aaaacgcagc gacgagcgcg gcgcaaatca ggcgcatgcc gtagtaaggc ttcatgggca
     2521 tcctcctcct tatggtttta ggtaatgtat atcataaggg acacgacccg gaaggcaaaa
     2581 ggcaaaaggc aaaagggaaa aggtaatagc tttcgatcca cgatccacga attttcaaat
     2641 gagaacgcag ctcgtaacca tccccaccga tacgatcccg ctcgaaggcg cgttccacga
     2701 gcctgacggg ggcgaggcag cgggcgccgt gctgctgttt cacggcaaca ccatgaactt
     2761 ctacgtcggc gcgccgcgct tcctgccgcc ggtgctgacg cgcctgggtc tcgcgtgcct
     2821 cgccttcaac cgccgcgggc acgatatcct cagcacgcgc aacagccggc aggccgaggg
     2881 cggcgcgttc cagttcacgc gcgaggcaat tgccgacaac cgcatcgccg ccgagtggct
     2941 cgcggcgcgc ggcttcccgg cgccggtcgt gatcgggcac agcaacggcg gcatgctcgg
     3001 cacgcagcac gtggtggacc acccgcgcac gcccgcactg gtgctgctct cgtccgggcg
     3061 cggcggcgcg agaccggtgt cgggcggcag ggaaggcttg tttgccggcg accgcgaggc
     3121 ggaaattacc gcgcaggcaa aatcgctgat ggaagccggg cgcgggcgcg agctgatgtt
     3181 catgcccggc tggtggtacg tgatcagcgc ggagagcttt ctcgaccggg tgaagacggt
     3241 gccggacacg atcgcgctcg cgccgaagat caagtgcccg gtgctcgcca tccgcggcga
     3301 caaggaagac ccggaacgct atccggcgga agaatacggc cgcgcggcgg gcgggccgtg
     3361 ccgggtggag atcattcccg actgcgacca cttctacaac ggccgcgagg accacgtcgc
     3421 gaacgtcgtc gcgtcgtggc tggctcagac gctacgcttg cctggagtca aaagctgaga
     3481 cacaaaggat ataaaggaca caaaggagac agaataaaaa acaaatcgaa ccgccaagac
     3541 cgccaagaaa atccatatac atgtagatat acctgcgcat ttgcatatat ctacatttgg
     3601 atagttccaa atacgggtat atgcttttgt cgtctaattc ttgccaacta atacaagtta
     3661 gcgggacacg ttaagatttc gattttcttg gtggccttgg cggttaatta ttgatctttg
     3721 tttttccttc gtgtcctttg tgtccttcgt gtcttcgcat tacgtcagat attcatctcc
     3781 aggatataca gcccgccggt aaagcggtcg ccggcataca cgatccgccg gtcgtcgacg
     3841 tagacgtcgt tgatctggat cgcgcccttg ggcgataacg gcggcgcgcc gggcacgaag
     3901 taggcgactt cgcgcggctg gtacggattg gacacgtcgt aggcgcgcac gccgccgttg
     3961 aagaacgtgg cgagcacgat attctccgag cgccacgagc aggggcccgg gtagttctcg
     4021 taaagattgt gcgagccgta gcggccgccg cgcttgccga acacctccac cggcggcagc
     4081 ggcagcgtgg aaatcggcac cgggttcttt tcgtcgcgga tgtcgaacac ccacgtcagc
     4141 ttcggccaat cctcgccgtc gtccttggtg cactcgtcgg agacgatcag caggcccttc
     4201 tcgaacagcg gcatcgcggt gtgcgtgaag ccgttgtagg gcggcgaata gttgtagcgc
     4261 gagatcatct tcggcttcga cttgtcgctg atgtcgagga tgacgagccc gccgtcgatg
     4321 taggacacgt aggcgcggtc cgggcgctgc gggtagcact gggtattgtg cgggcggtag
     4381 ccgccgtcga accggggatg gcgcggcagc ggcggctcgg cgtcgccctc gcgctgaccg
     4441 ggataccacc agcggcccgc ttcgaccggc ttcgagggat tgcgcacgtc gatgatgcgg
     4501 tagaactggt ggtccttggg gtcgcgcggc tggaagtcgg cggcgcccga ggaaatatgc
     4561 aggtactcgc cgtcgccggt ccacacgcaa tgcacgccca gcgagtgggg gccggagcag
     4621 tcgaaatgag tgatcttccg gggctgctcg ggcctggcta tgtcccagat gtcgaacccg
     4681 gccggcgtca tgccgcgctc cccgcggatc tggtgcgcga cgtacagcgt gtcgccgaac
     4741 acttccagcg agttggagcg catcttgccg tggggcagct ccgtctggat gatcatcttc
     4801 gggttgcgcg ggtcggtgac gtcgatcgcc gtgaaattct tgggcgcggc ctcgtgcccg
     4861 agccagatca cgcgccgtcc gtccttggtc ttctgcatgc ccatgccttc gcccatgccg
     4921 ccgaacccgc ccatgtcgtg gtgaccgagc agcttcatgt tgagcgccag cgtctggctc
     4981 gcctgcctgc cgggttgcat ctttccgtct gccgtcattt tctctccctt gatttaggtg
     5041 tcagccagct gaaaaaacat tagccacgga atcacacgga aatacacgga attcaaatct
     5101 tgaaaaagtc tcgccttgcg acgttttgtt tttccgtagc ttccgtgtgt gtccgtggct
     5161 aaataagact ttttgagacc cgtttcattc cttcgctagt ccggcgtcga ccagcgcgcc
     5221 cttcagctgc tcgtagatgg cggccatgcg ctgcgggctc tgcctgctgc ccaggtagtc
     5281 ggtgacaccg tgctgcttgc cgaccgcgct cttccactct tcgctctgga ccacgcgctc
     5341 gagcgccgtg tcccagtacg ccacttgcgc cggcgtcatc cccctgggcc cgatcagcag
     5401 gcgcgtattg gtgaaaacga cgtcgaagcc ctgttctttc cacgtcggga tcgaagccag
     5461 cgggccgtcg ccgcggcgat cggacgtgat gccgacgatg cgaagctggc cggccgcggc
     5521 ggcgcgcagc gccgcgccca ccgagagcgg cgccagatcg acgtgcccgc ccatgacgga
     5581 cgtgacggtc tgcgcgttcg cctggaacac cacggtcttc agcttcctga cgtcgacgcc
     5641 catggatttc atcagcagcg cgacggtgag gtggttcgtc gcgccgatcg ccacgccgat
     5701 cgcgatgttg agggactggg gatcccgctt cagggcttcc tggatgtcgc ggccggtctt
     5761 gagcggggaa tccggcctga ccacgaacgt catgtgctcg ctgaacagcg tcgcgagcgg
     5821 ggtgtagtc
//
LOCUS       DBNGSG010000416         7972 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_474475, whole genome shotgun sequence.
ACCESSION   DBNGSG010000416 DBNGSG010000000
VERSION     DBNGSG010000416.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7972)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7972)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7972
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_474475"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="AC158Q_10855"
     CDS             complement(<1..323)
                     /locus_tag="AC158Q_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340315.1"
                     /translation="MPEKILKVKLVGLRTAAGLALVLALAAAYWLLHDSELVAAILDA
                     GALKAHVVRLGPLGPIAVIGLMVLAILVSPIPSAPIALAAGALYGHTWGTVYVLLGAE
                     IGALAA"
     gene            467..844
                     /locus_tag="AC158Q_10860"
     CDS             467..844
                     /locus_tag="AC158Q_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340316.1"
                     /translation="MRSRRSLRFLGIVALTAMVFAQAAFALAACDDYRAQSRALLAAT
                     EQAETIGCHEAEVNANICLAHCQGRDQTLDKHRVNVPAASLHPVLTIRSGQPARQPDL
                     VFARLPQAIAAPPARILFQSLLI"
     gene            981..2225
                     /locus_tag="AC158Q_10865"
     CDS             981..2225
                     /locus_tag="AC158Q_10865"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR3340317.1"
                     /translation="MALLLAALPVRLATAQEAVDPQLAALLAEAGENNPDVQAARREA
                     NAARSRISPAGALDDPMLEAGVLNYPVESRSFKTEDMTMKMIGFTQRLPYPGKRALRR
                     DLAEQEALAVEANLQELANRVRRDVKVAYFDLGFVDESLRLAERNRRVLEQYLSIAEA
                     RYGVGQGTQADVLKAQTQVSKMLEELIKLGRERPMLEAELNRAAGRGGAPGSVTPPPA
                     QARDVALALEHLRAAARESRPQLAAQLRMMGRNAKALELARKDYYPDFDVRVSYGQRD
                     NSPDMRREDMISFTVAINLPVWRESKRDPRVTEAEAMREQASAMYQARLNELDAMLRQ
                     QVAAAEQSLKSVRLYETGLLPQARLTADASLAAYRVGRVDFFSLLDSRMTVFNAEVGY
                     AASLAAYNKALAEIEFLVGKSL"
     gene            2234..3751
                     /locus_tag="AC158Q_10870"
     CDS             2234..3751
                     /locus_tag="AC158Q_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3340318.1"
                     /translation="MAIAAGALAVTAALAAGYWLGGRGGSHPEAAAPAAGSSSPKSER
                     KVLYYRNPMGLPDTSPVPKKDPMGMDYIPVYEGEEAPAKAGTVKISLDKVQLLGVRTE
                     RAEPRELRRTVRAVGTIQANERLLYKVAPRFEGWIEKLLVNTTGQAIARGEPLMDVYS
                     PELITAQEEYLIALRGLKEAAAGGPEAEAVMQRLAESALRRLRNWEISDQELRSLQRE
                     GKARQRLVYRSPVKGVVLQKPSMQGMRFMPGEVLFEVADLSLVWMLAEVFEQDLRLVR
                     VGQDVTLHIASYPDKAFSGKVVFIYPTIDPGTRTARVRVELPNPGHLLKPAMYGQVEL
                     AARHPRGKALAVPDSAVLDSGNRQAVLVRRGEGLFEPRTVKLGMRADGYIEVSEGLQA
                     GDEVVVRANFLIDAESNLKAALEAFGGQAKSGQHRGTGTVQAVDPKDGSVEISHEPIP
                     SLNWPAMSMEFKVKDKALLAGLKPGQSVEFDLTQLKPGEYLVERIAPTGAAHKGH"
     gene            3767..6988
                     /locus_tag="AC158Q_10875"
     CDS             3767..6988
                     /locus_tag="AC158Q_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3340319.1"
                     /translation="MLSRIIDWSARNVFLVLLATFFIVGGGVYAVLKTPVDAIPDLSD
                     VQVIVYTEYPGQAPQVVEDQVTYPLTAAMLGVPKSKVVRGLSVFGASFVYVIFEDGTD
                     IYWARSRVLEYLNYVSGRLPKGVTASLGPDATGVGWVYQYAVMAKNRSLEELRTIQDW
                     FVRYQLTKAHGVAEVASVGGFVKQYQVTVNPRLLQAYGVPLTKVIQVIRDSNRDVGGR
                     VVEMTETEYMVRGRGYLRGMADIENVVVKADAGTAVLIRDIARVELVPDERRGIAELD
                     GQGEVVGGIAVARYGHNAQEAIDNLKAKVSEISTGLPEGVAIKTVYDRSELIQRAIKT
                     LNWTLLEESLIVALVCIVFLFHVRSALVAILVLPIGILIAFIVMRYIGLNSNIMSLGG
                     IAIAIGAMVDAAIVMIENAHKHLERLKPGDSRLEAIITACREVGPALFFSLLIITVSF
                     LPVFTLEAQEGRMFSPLAYTKTFAMAGAAFLSITLVPVLMLLFIRGRILPEHRNPVVR
                     FLIWIYRPVIGWVLHWRKLTLLAAAVTLGITAYPLSRLGTEFMPTLNEGTLLYMPSTL
                     PSLSVTKAAELLQIQDRIIKSFPEIESVFGKAGRANTATDPAPTEMFETVINLKPPSE
                     WRPGMTMDKLIAELDQALQFPGIANAWTMPIKARIDMLSTGILTPIGIKVFGKDLAEI
                     ERVAKQVEAVVKAVPGTTSAYAERLTGGYYIEIEPDRMALARYGLAVGELQEVIATAL
                     GGEMVTTTVEGLERYGVVVRYPRDFRSDLNAIATQVLVPVMNGGGAVAMGARAPVMNA
                     AASGGPAAAQLKTVSGTMLPLGQLASIKLVKGPPNIRTENALLAAYIYVDIRDRDIGG
                     YVADAQKAVRENVKFPPGYYATWSGQFEYMERAKEKLKVVVPLTLVIIFLLLYLNFRR
                     LTETLIVMLSVPFSLVGGVWLMYLLDYHMSVAAAVGFIALAGVAAETGVVMLIYLDQA
                     FAAMTDKRRTARERVTAADLHEAVMQGAALRVRPLMMTVFAIIAGLLPIMWGSGTGSE
                     VMRRIAAPMVGGMVSATILTLIVIPAIYALVKQFAIRPASEPA"
     gene            6997..7341
                     /locus_tag="AC158Q_10880"
     CDS             6997..7341
                     /locus_tag="AC158Q_10880"
                     /inference="COORDINATES: protein motif:HMM:NF012753.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HIR3340320.1"
                     /translation="MDIKAVIAFIRRDKLEDVESSLRQIGVERINVYKVKGYGEYHDF
                     FARDWMVDEVRIDIFTRADEVDAIVAAIMGAAHTGLPGDGVVAVIPVEKFYLIRTRSE
                     ATPAEFWPRAKA"
     gene            7350..>7972
                     /locus_tag="AC158Q_10885"
     CDS             7350..>7972
                     /locus_tag="AC158Q_10885"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3340321.1"
                     /translation="MAPEQGFYGRHVLPHLVHLACSLKPIRRQREKVVPMARGRVLEI
                     GVGSGLNLAYYDPGKVSRLFGLDPSAEMIAKAKRAARSSSLEIEFLNAQAESIPLESK
                     SVDTVLVTYSLCTIPQTEPALKEMRRVLKPGGKLVFCEHGLAPDPNVRKWQDRITPFW
                     RRFAGGCHLNRDIPALIEQGGFRIEDMESMYLPGWRPATFNYWGTAER"
BASE COUNT         1407 a         2561 c         2698 g         1306 t
ORIGIN      
        1 gcggcgaggg cgccgatttc cgcgccgagg agcacgtaaa ccgttcccca ggtgtgcccg
       61 tacagggcgc cggcggcgag cgcgatggga gcgctcggga tcgggcttac caggatcgcg
      121 agaaccatca gaccgatcac cgctatcggt cccaaggggc ccagcctgac aacgtgggcc
      181 ttgagtgccc cggcgtcgag gatcgctgcg accagctccg aatcgtgcag cagccagtag
      241 gccgctgcca gcgccagtac gagggccagc cctgccgccg ttcgcaatcc aaccagcttc
      301 accttgagaa tcttttccgg catcggtccg agccgagttg atgcacgtca attgctcgac
      361 gacaaacggc aaaccgagga tcaatgctcg cttcatgcac aagtcgctgc gctcggtgtc
      421 gtgaaactaa gcagggggtg cataatgtct cggctgattg cccatcatgc gctcccgccg
      481 ctcgctgcgt ttcttaggta tcgtcgcctt gacggcgatg gttttcgccc aggcagcgtt
      541 cgccttggcg gcctgcgacg actatcgcgc gcaatctcgt gcgcttctcg ccgccacgga
      601 gcaagccgaa accattggct gccacgaggc ggaggtcaac gccaacatct gtctcgctca
      661 ttgccagggc agggatcaga ccctcgacaa gcaccgcgtc aacgtgcccg cggcctcgct
      721 gcaccccgtg ttgacgatcc gcagcgggca gccggcgcgt cagccagacc tcgtcttcgc
      781 gcgcctgccg caggcaattg ccgccccgcc ggcgcgcatt ctctttcagt cgctgcttat
      841 ctgagcggct tgccgccgtc ggcggcgagt tgatttcctg gattcccgca acgcggctgc
      901 gttgccgggc ggcctaaaca gacggggggt gagagatgca gagatcgatt tgcgcaaaat
      961 tcgggtttct atcggcggcc atggcactgt tgctggcggc attgcccgtt cgcctggcga
     1021 cggcgcagga agccgtcgat ccgcagctcg cggcgctgct cgccgaggcg ggagagaaca
     1081 atcccgacgt ccaggcggcg cgccgcgagg cgaacgcggc acgcagcagg atctccccgg
     1141 ccggcgcgct cgacgacccg atgctggaag ccggcgtgct caactatccc gtcgagtccc
     1201 ggagcttcaa gaccgaggac atgacgatga agatgatcgg ctttacccag cgcttgccgt
     1261 accccggcaa gcgcgcgctg cgccgcgacc tcgcggaaca ggaggcgctc gccgtcgagg
     1321 ccaacctcca ggagctcgcc aaccgcgtgc ggcgcgacgt caaggtagcg tacttcgatc
     1381 tcggcttcgt cgacgaatcg ctgcgcctgg ccgagaggaa ccgccgcgtg ctggagcagt
     1441 acctgtcgat cgccgaggcg cgctacggcg tggggcaggg cacgcaggcc gacgtgctga
     1501 aggcgcagac gcaagtgtcg aagatgctcg aggagctgat caagctcggc cgcgagcgtc
     1561 ctatgctcga ggcggagctt aaccgcgccg caggccgcgg cggggcgcca ggcagcgtca
     1621 cgccgccgcc ggcgcaggcc cgcgacgtcg cgctcgcgct agaacacctg cgcgccgcgg
     1681 cacgcgagag ccgcccgcaa ctcgccgcgc agctgcgcat gatgggtcgc aacgccaagg
     1741 cgctcgagct ggcgcgcaag gactattacc cggacttcga cgtgcgggtg tcctatggcc
     1801 agcgcgataa ctccccggac atgcgccgcg aggacatgat cagcttcacc gtggcgatca
     1861 acctgccggt gtggcgcgag tccaagcgcg atccgcgggt cacggaagcc gaagcgatgc
     1921 gcgagcaggc ttcggcgatg taccaggcgc ggctgaacga gctcgacgcc atgctgcgcc
     1981 agcaggtcgc cgccgccgag caatcgttga agtcggtgcg gctttacgag accgggctct
     2041 tgccgcaggc gagactcacc gccgacgcgt cgctcgccgc ctatcgcgtc ggccgcgtcg
     2101 acttcttcag cctcttggac agccgcatga cggtgttcaa cgccgaggtc ggctatgcgg
     2161 cgagcctcgc cgcttacaac aaggcactcg cggagatcga gttcctggtg gggaaatcgc
     2221 tatgaaccga acgatggcaa tcgcagccgg agcgctcgcc gttaccgcgg cgctcgccgc
     2281 aggctactgg ctcggcgggc gcggcggcag ccatccggaa gcggccgcgc ccgccgcggg
     2341 atcgagctcg ccgaagagcg agcgcaaggt cctctattac cgcaatccca tggggctgcc
     2401 cgacacctcg ccggtgccga agaaggaccc gatggggatg gattacatcc cggtgtacga
     2461 aggcgaggag gcgccggcga aggccggcac ggtgaagatc agcctcgaca aggtgcagct
     2521 actcggcgta aggaccgagc gcgccgagcc gcgcgagctg cgccgcacgg tgcgggcggt
     2581 cgggaccatc caggcgaacg agcggctgct ctacaaggtc gcgccgcggt tcgaaggctg
     2641 gatcgaaaag ctcctcgtca acaccaccgg ccaggcgatc gcgcggggcg agccgctcat
     2701 ggacgtctac agcccggaac tcatcaccgc ccaggaggaa tacctcatcg cgctgcgcgg
     2761 cctcaaggaa gccgccgcgg gcggacccga ggctgaggcg gtaatgcaac gcctcgccga
     2821 gagcgcgttg cgccggctgc gcaactggga aatctccgac caggaattgc gctcgctgca
     2881 gcgggagggc aaggcgcgcc agcgcctcgt gtaccgctcg cccgtgaagg gcgtcgtgct
     2941 gcagaagccg agcatgcagg ggatgcgctt catgcccggg gaggtgctgt tcgaggtcgc
     3001 cgatctgtcc ttggtatgga tgctcgccga ggtcttcgag caagaccttc gcctggttcg
     3061 cgtcggccag gacgtgaccc tgcacatcgc gtcctatccg gacaaggcgt tctccggcaa
     3121 ggtggtgttc atctatccga ccatcgatcc ggggacccgc accgcgaggg tccgcgtcga
     3181 gctgcccaat cccggccacc tgctcaaacc cgcgatgtac ggccaagtcg agctcgccgc
     3241 gcgccacccg cggggcaagg cgcttgcggt gcccgattcg gcggtgctcg acagcggcaa
     3301 ccgtcaagcg gtcctggtgc ggcgaggcga ggggctgttc gagccgcgca cggttaagct
     3361 cggcatgcgc gccgatggct acatcgaagt gagcgagggc ctgcaggctg gcgacgaggt
     3421 cgtggtgcgg gcgaacttcc ttatcgatgc cgagagcaat ctcaaggcgg cgctcgaggc
     3481 cttcggcggg caggcgaaaa gcggccaaca ccgaggaacg gggaccgtgc aagcggtcga
     3541 tccgaaagac ggcagcgtcg aaatcagcca cgagccgatc ccgagcctga actggccggc
     3601 gatgtcaatg gagttcaagg tgaaggataa ggcgctgctc gcagggctca agccgggcca
     3661 gtcggtggaa ttcgatctca cgcagctcaa gccgggcgaa tacctggtcg agcgcattgc
     3721 gccgacgggc gccgcgcaca aaggacactg agatgaggca attgacatgc tgagccgcat
     3781 catcgactgg tcggcgcgca acgtctttct cgtgctgctg gcgacgtttt tcatcgtcgg
     3841 cgggggcgtg tacgcggtgc tcaagacgcc ggtcgacgcg attcccgatc tttccgacgt
     3901 gcaggtgatc gtctacacgg aatacccggg gcaggcgccg caggtcgtgg aagaccaggt
     3961 gacctatccg ctgaccgccg ccatgctcgg cgtgcccaaa tcgaaggtgg tgcgggggct
     4021 gtcggtcttc ggcgcctcgt tcgtgtacgt catcttcgag gacggcaccg acatctactg
     4081 ggcgcgctcg cgcgtgctcg agtatctcaa ctacgtttca ggtcggctgc cgaagggcgt
     4141 tacggcgtcg ctcgggccag acgccaccgg ggtgggttgg gtgtaccagt acgccgtgat
     4201 ggcgaagaac cgctcgctcg aggagttgcg cacgatccag gactggttcg tgcgctacca
     4261 gctcaccaag gcgcacggcg tggccgaagt cgcgagcgtc ggcggctttg tcaagcaata
     4321 ccaggtgacg gtcaatccgc gtctgttgca ggcctacggc gtgccgctga ccaaggtcat
     4381 ccaggtcatc cgcgacagca accgcgacgt gggcggccgc gtggtcgaga tgaccgagac
     4441 cgagtacatg gtgcgcggcc gcggctatct gcgcggcatg gcagacatcg agaacgtcgt
     4501 ggtgaaagcc gacgccggca ctgcggtgct gatccgggac atcgcgcgcg tcgagctggt
     4561 gcccgacgag cggcgcggca tcgccgagct cgacggccag ggcgaggtgg tcggcggcat
     4621 cgccgttgcg cgctacggcc acaacgcgca ggaggcgatc gacaatctga aggccaaggt
     4681 ctcggagatc tcgaccggac tgcccgaagg ggtagcgatc aagacggtgt acgaccgctc
     4741 ggaactgatc cagcgtgcga tcaagacgct caactggacg ctgctcgaag agagcctgat
     4801 cgtcgccctg gtatgcatcg tgttcctgtt ccatgtccgc agcgcgctgg tggcgatcct
     4861 cgtgctgccg atcgggatac tgatcgcgtt catcgtcatg cgttacatag gactcaactc
     4921 gaacatcatg agcctgggcg gcatcgcgat cgccatcggt gccatggtgg acgcggccat
     4981 cgtgatgatc gagaacgcgc acaagcacct ggagcgcctg aagcccggcg actcgcgcct
     5041 ggaggcgatc atcaccgcct gccgcgaagt cgggccggcg ctgttcttca gcctgctcat
     5101 catcacggtg tcgttcctgc cggtgttcac gctggaggcg caggagggac gcatgttcag
     5161 cccgctcgcc tacaccaaga ccttcgcgat ggccggcgcc gcattcctgt cgatcacgct
     5221 ggtgccggtg ctgatgctgc ttttcatccg cggacgcatc cttcccgagc accgcaaccc
     5281 ggtcgtgcgg ttcttgatct ggatctaccg gccggtgatc ggctgggtcc tccactggcg
     5341 caagctcacc ctgctggccg ctgccgtcac gctcggcatc accgcttacc cgctgtcgag
     5401 gttgggcact gagttcatgc cgaccctgaa cgaagggacg ctcctctaca tgccgagcac
     5461 gctgccgtcg ctgtcggtga ccaaggcggc cgagctgctc cagatccagg accggatcat
     5521 caagagcttt cccgagatcg agtcggtgtt cggcaaggcc gggcgcgcca acacggccac
     5581 cgacccggcg ccgaccgaga tgttcgagac cgtgatcaat ctcaagccgc caagcgagtg
     5641 gcggccgggc atgacgatgg acaagctcat cgccgagctc gaccaggcgc tgcagttccc
     5701 gggaatcgcc aacgcctgga ccatgccgat caaggcgcgc atcgacatgc tctcgaccgg
     5761 gattctcacg ccgatcggca tcaaggtgtt cggcaaggac ctggcggaaa tcgagcgggt
     5821 ggcgaagcag gtggaagcgg tggtgaaagc cgtgcccggc accaccagcg cctacgccga
     5881 gcggctcacc ggcgggtact acatcgagat cgagcccgac cgcatggcgc ttgcccgcta
     5941 cggcctggcg gtcggcgagc ttcaggaagt gatcgccacc gcgctcggcg gcgaaatggt
     6001 caccacgacg gtcgaaggcc tcgagcgcta tggcgtcgtg gtgcgttatc cgcgcgactt
     6061 ccgcagcgat ctgaacgcca ttgcgacgca ggtgctcgtt ccggtgatga atggcggcgg
     6121 ggccgtcgcc atgggcgctc gggcgccggt catgaacgct gccgcttcgg gcggcccggc
     6181 cgcggcgcag ctcaagaccg tgagcggaac gatgctaccg ctcgggcagc tggcgagcat
     6241 caaactggtc aaggggccgc cgaatatccg cacggagaac gcgctgctcg ccgcctacat
     6301 ctacgtcgat atccgcgatc gcgacatcgg cggctacgtc gccgacgcgc agaaggcggt
     6361 gcgcgagaac gtcaagttcc cgccgggcta ctacgccacc tggagcgggc agttcgagta
     6421 catggagcgg gccaaggaaa agctcaaagt cgtcgtgcct ctgaccctcg tgatcatctt
     6481 cctcctgctc tacctcaatt tcaggcggct gaccgaaacg ctgatcgtca tgctgtcggt
     6541 gccgttctcg ctggtcggcg gcgtgtggct gatgtatctg ctcgactacc acatgagcgt
     6601 ggcggcggcc gtgggattca tcgcgctcgc gggagtcgcc gcagaaacgg gggtggtcat
     6661 gctgatctat ctcgaccagg cgttcgccgc catgaccgac aagcggcgca cggcgcgcga
     6721 gcgggtgacg gcggctgatc ttcatgaggc cgtcatgcag ggcgcggcgc tgcgagtgcg
     6781 accgttgatg atgacggtgt tcgccatcat cgccgggctg ctgccgatca tgtggggcag
     6841 cgggaccggc tcggaggtga tgcggcgcat cgcggcgccg atggtcgggg gcatggtctc
     6901 ggccaccatc ctcacgctca tcgtgatccc ggcgatctac gcgctcgtga agcaattcgc
     6961 catccgccct gccagcgaac cggcgtgaga ggaaacatgg acatcaaagc agttattgca
     7021 ttcattcgcc gcgacaagct cgaggacgtc gaaagcagtc ttcggcagat cggcgtcgag
     7081 aggatcaatg tctacaaagt caaggggtac ggcgagtacc acgatttctt cgcgcgcgac
     7141 tggatggtcg acgaagtcag gatcgatatc ttcacgcgcg cggacgaggt cgatgcgatc
     7201 gtggcggcga tcatgggcgc tgcgcacacc ggcttgccgg gagacggcgt ggtcgccgtg
     7261 atcccggtcg agaagttcta tctgatccgc acgcgctcgg aggcgacgcc ggccgagttc
     7321 tggccgcgag ccaaggcgtg agcggtgctg tggccccaga gcagggcttc tatggccggc
     7381 acgtcttgcc gcatctcgtc catcttgcat gcagcctcaa gccgatcagg cgccagcgcg
     7441 agaaggtggt tccgatggcg cgaggccgcg tgctggaaat cggtgtcggc tcggggttga
     7501 acctcgctta ctatgatccc ggcaaggtct cgcgactgtt cggcctcgat ccttcggccg
     7561 agatgatcgc gaaggcaaag cgggccgccc gttcctcaag cctggagatc gaattcctca
     7621 acgcgcaggc ggagagcatt ccgctggaga gcaagagcgt cgatacggtt ctggtcacgt
     7681 acagcctgtg caccatcccg cagacggagc ccgccctgaa agagatgcgc cgggttctga
     7741 agcccggcgg caagctggtt ttctgcgagc acgggctcgc gcccgacccg aacgtgcgca
     7801 aatggcagga caggatcacg ccgttctgga ggcgattcgc aggcggctgc catctgaacc
     7861 gcgatatccc ggcgctgatc gagcagggcg gattccgcat tgaggacatg gaaagcatgt
     7921 atctgcccgg ctggcggccg gcgactttca actactgggg tacggccgag cg
//
LOCUS       DBNGSG010000417         4351 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4379831, whole genome shotgun sequence.
ACCESSION   DBNGSG010000417 DBNGSG010000000
VERSION     DBNGSG010000417.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4379831"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="AC158Q_10890"
     CDS             complement(<1..412)
                     /locus_tag="AC158Q_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HIR3340322.1"
                     /translation="MKAAALGTGLPQGWRRVAAALLLTLACGCAALRPAVSPHPNFYS
                     LDGARDAAAPEQPGPRAAATAPTLTVSPPHAAAGFDSRRIMYVRQADQLEYFAHNEWV
                     DTPARMLAPLIVAAVGSGGAFNAVVLTPSPASGEM"
     gene            complement(409..1335)
                     /locus_tag="AC158Q_10895"
     CDS             complement(409..1335)
                     /locus_tag="AC158Q_10895"
                     /inference="COORDINATES: protein motif:HMM:NF014523.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HIR3340323.1"
                     /translation="MNYVAVGAFVLVLGSALIAGVLWLAAGGALQKKYDLYLAIENES
                     VAGLNLNAPVKYNGVDVGKVRKIWLEPGNPEQVNLLFAIERGTPIKEDTVAVLKTQGL
                     TGIAYVEMSGGARDSPPLVAVAGNDYPVIRTKPSLSTRLENVLTSVLAKLDNTSSSIN
                     AILSDENKAAFKSSLADIAAVTRTISGRKEAIDKVIVSAARTFDNSARATEQLERVVA
                     RIGKSAESVEKMGDEVARASASAGKTVDSVGADVARFTAEALPEFERLLGEMSVLAAS
                     LRRLSEKMEGSPGGLLMGRRSVPPGPGEATAK"
     gene            complement(1332..2159)
                     /locus_tag="AC158Q_10900"
     CDS             complement(1332..2159)
                     /locus_tag="AC158Q_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3340324.1"
                     /translation="MQSEAVIEASKVSTRFGDHVVHDELDLEVRRAEIFALIGGSGSG
                     KSTLLREMILLQRPDSGSIRVLGADLQKLGDEEALALRRRWGVMFQHGGLFGSLTVKE
                     NIGLPLREHTGLEDALIDEIAAWKLAMTGLEPEAGAKYPSELSGGMMKRASLARALAL
                     DPELLFLDEPTAGLDPDSAGGVDQLVRKLRDLFGLTIVMITHDLDLLWQVADRVAVLA
                     EGKVQGIGSMSELYQMDSPAIRMFFDGPRGRAAQEQVRQQAGARSGETTGRPSSKPR"
     gene            complement(2161..3282)
                     /locus_tag="AC158Q_10905"
     CDS             complement(2161..3282)
                     /locus_tag="AC158Q_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3340325.1"
                     /translation="MAPAALAQPTPQEITLSGPWTARGIGAVGPQLDALCAQPRSEMV
                     VDGARIEALDTAGAWVLQKLLRRLRGEGTAVQVRNLRPEFARLLEAVGQQAADRTPSP
                     APSAGASPSMLESLGRSAEAALEQGAALLGFVGETAVALAGCVAHPARFRWRPILYNI
                     RSAGFDALPIVGLLSFLLGIVIAYQGADQLRQYGANIFVADLVGVSMLREFAPLITAI
                     IIAGRSGSAYAAQIGTMSVTEEIDAMRTLGIAPLELLVLPKILALVIALPLLTVFADV
                     LGVFGGMIMAQAQLGVGFGEFLDRFVKAVSITTYLVGIGKAPVFAAIIAVVGCFQGFR
                     TRGGADSVGRQTTRAVVHSIFLVIVADALFSIAFSALDL"
     gene            3474..3785
                     /locus_tag="AC158Q_10910"
     CDS             3474..3785
                     /locus_tag="AC158Q_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIR3340326.1"
                     /translation="MKLFKHLSAVLLALAFATVLGCAGGPQKESTGEYFTDSWITTKV
                     KSALAADKQVKATEVNVETFKGRVQLSGFVSSNAAMNQAVVVAQGIKGVTSVQNDMRI
                     R"
     gene            4137..>4351
                     /locus_tag="AC158Q_10915"
     CDS             4137..>4351
                     /locus_tag="AC158Q_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340327.1"
                     /translation="MRYVIIVLWSLIWSVTSATAQVSIGIGLPNVSIGINLPLYPELV
                     PVPGYPVYYAPRVSSNYFFYDGMYWVY"
BASE COUNT          737 a         1426 c         1463 g          725 t
ORIGIN      
        1 tcatctcgcc cgacgcgggg ctcggcgtca gcaccacggc attaaacgcc ccgccgctgc
       61 ccacggccgc gacgatcagg ggcgccagca tgcgcgccgg cgtatcgacc cactcgttgt
      121 gagcgaagta ttcgagctgg tcggcctggc gcacgtacat gatgcgccgg ctgtcgaagc
      181 cggctgccgc gtgcggcggg ctgacggtca gcgtcggcgc cgtggccgcg gcgcgcggcc
      241 ccggttgctc aggcgccgcg gcgtcgcgcg cgccatcgag cgagtagaag ttcggatgcg
      301 gcgagaccgc cggccgcagt gccgcgcaac cgcaggcgag cgtcagcaac aacgcggcgg
      361 ccacgcgccg ccacccctgc ggcaggccgg tcccgagcgc ggcagctttc atttcgccgt
      421 cgcctcgccc gggccgggcg gcaccgaccg gcggcccatc agcaggccgc cggggctgcc
      481 ttccattttt tcgctcaggc gccgcagcga ggccgccagc acggacatct cgccgagcag
      541 gcgctcgaac tcgggcaggg cttccgcggt aaagcgcgcc acgtcggcgc cgaccgagtc
      601 gaccgtcttt ccggcgctgg cgctcgcgcg ggccacctcg tcgcccatct tctccacgga
      661 ctccgcgctc ttgccgatgc gcgcgaccac gcgttcgagt tgctcggtcg cgcgggcgct
      721 gttgtcgaag gtgcgcgccg cgctgacgat gaccttgtcg atcgcttcct tgcgcccgga
      781 gatcgtgcgt gtcacggcgg caatatcggc gagtgagctc ttgaaggccg ccttgttctc
      841 gtcgctgaga atggcattga tgctgctcga agtgttgtcc agtttcgcca ggacgctggt
      901 gagcacgttt tcgagccgtg tgctgaggga tggcttggtg cggatcaccg ggtaatcgtt
      961 tcccgcgaca gcgacaagcg gcggagagtc ccgggcgcct ccgctcatct cgacataggc
     1021 tatgcccgtc aggccctggg ttttcaggac cgccacggtg tcctccttga tcggcgtgcc
     1081 gcgctcgatg gcgaagagca ggttcacctg ttccgggttg cccggctcta gccagatttt
     1141 gcggaccttg cccacgtcga cgccgttgta cttgaccggc gcgttcaggt tgaggccggc
     1201 caccgactcg ttctcgatcg ccaggtacag gtcgtatttc ttctggagtg cgccgcccgc
     1261 ggcgagccag agcacgccgg cgatcagcgc ggaaccgaga accagcacga acgcgccgac
     1321 ggccacgtaa ttcaccttgg tttcgatgac ggcctcccgg ttgtttcgcc ggatcgcgct
     1381 ccggcctgct gcctgacctg ctcctgcgcc gccctcccgc gggggccgtc gaagaacatc
     1441 cggatggcgg gactgtccat ctggtagagc tcggacatcg agccgatgcc ctgcaccttg
     1501 ccctcggcga ggacggcgac gcggtcggca acctgccaca acaggtcgag gtcgtgagtg
     1561 atcatcacga tggtcaggcc gaacaggtcg cgcagcttgc gcacgagctg gtcgacgccg
     1621 ccggcgctgt ccgggtcgag gccggcggtc ggctcgtcga gaaacagcaa ttccggatcg
     1681 agcgccagcg cgcgcgccag cgacgcccgc ttcatcatgc cgccgctcaa ttcggacggg
     1741 tatttcgcgc cggcctccgg ttcgaggccg gtcatcgcga gtttccatgc tgcgatctcg
     1801 tcgatcaacg cgtcttcaag cccggtgtgc tcgcgcaacg gcaggccgat gttttccttc
     1861 accgtgagcg agccgaacag gccgccatgc tggaacatca ccccccagcg ccggcgcagc
     1921 gcgagggctt cttcatcccc gagcttctgc aggtcggcgc cgagcacgcg gatggagccc
     1981 gagtccgggc gctgcagcaa aatcatttcg cgcagcagcg tcgacttgcc ggagccgctg
     2041 ccgccgatca aggcgaaaat ttccgcgcgg cggacttcga ggtcgagttc gtcatgcacg
     2101 acgtggtcgc cgaagcgggt cgacaccttg cttgcctcga tgaccgcttc gctttgcacg
     2161 ttagaggtcg agcgcgctga aggcgatcga gaacagggcg tcggcgacga tcacgaggaa
     2221 gatcgagtgg acgacggcgc gcgtggtctg gcggccgacg ctgtcggcgc cgccccgggt
     2281 gcgaaatccc tggaagcagc cgaccacggc gatgatggcg gcgaacaccg gcgccttgcc
     2341 gatgccgacc aggtaggtgg tgatgctgac cgccttgacg aagcggtcga gaaactcgcc
     2401 gaatccgacg cccagttgcg cttgcgccat gatcatgccg ccgaacacgc cgagcacgtc
     2461 cgcgaacacg gtcagcagcg gcagcgcgat caccagcgcc aggatcttcg gcaataccag
     2521 cagttccagc ggcgcaatgc cgagcgtgcg catggcgtcg atctcctcgg tcacggacat
     2581 cgtgccgatc tgcgcggcat aggcggaacc cgagcgcccg gcgatgatga tggccgtgat
     2641 caggggcgcg aactcgcgca acatggacac gccgacgaga tcggcgacga aaatgttggc
     2701 gccgtattgc ctcagctggt cggcgccttg ataggcgata acgatcccga gcaggaacga
     2761 cagcagcccg acgatgggca gcgcatcgaa tccggcgctg cgtatgttgt acaggatggg
     2821 gcgccaccgg aaccgcgccg ggtgcgcgac gcaacccgcc agggcgaccg cggtttcccc
     2881 gacgaagccg agcagcgcgg cgccctgttc cagggcggct tcggcgctcc gtcccaggct
     2941 ttccagcatc gatggggacg cgccggcgga cggcgcgggg gagggtgtcc gatcggcagc
     3001 ctgctgcccc acggcttcaa gcagtctggc gaattccggg cgcaggttgc gcacctgcac
     3061 ggcggtgcct tcgccgcgca gccggcgcag gagtttttgc aggacccaag cgccggcggt
     3121 atcgagagcc tcgatgcgcg cgccgtcgac gaccatctcc gaccggggct gcgcgcacag
     3181 cgcgtcgagt tgcggcccga cggcgccgat cccgcgcgcc gtccagggtc ccgacagggt
     3241 gatctcctgc ggcgtcggtt gagcgagtgc ggcgggtgcc accgacaaag ggtccatgat
     3301 gaaccggagg gtagatgaaa cttattggcc cgtttgtgcg ccagcgtacg gaacgccctc
     3361 atggaagggg cgacaatcgc ggtctcgtcg gggcttgcgg caacggcacg tcgtgccgct
     3421 atcgaagccg gcacgagctt tattcaccca ctttcagcct ggagaaacac accatgaaac
     3481 tattcaagca cctgtccgca gtcttgctcg ccctggcatt cgcgaccgtt ctgggttgcg
     3541 cgggcggccc gcagaaggaa agcaccgggg agtacttcac cgacagctgg atcacgacga
     3601 aagtgaaatc ggccctggcc gcggacaagc aggtcaaggc aaccgaagtc aacgtcgaaa
     3661 ccttcaaggg cagggttcag ttgagcggct tcgtgagctc caatgcggcc atgaaccagg
     3721 cggtcgtcgt cgcgcagggc atcaagggcg tgacctcggt ccagaacgac atgcgcatca
     3781 ggtagggcaa cccggcagga caaatgaggg gagccgtccc cttgtccgga tcaggggccg
     3841 tttcggtcat tccgagacgg ccttctggcg tactcgaacc gacacgcggg acgcgcgacc
     3901 cggctcgagg gcaaggcctc gcgcagtcgc cgcgaaactt tgcggcgaga acggctgtcc
     3961 ggccgccctg tgtacgatgg cgaacagagt gtcccgcgtg gaaccggata gtctccggcg
     4021 ggagccaatt ggaatgattg gggtctaaag aacgggcagc aggttggaag aaccggggcc
     4081 ggtgtcgtcg tcagcagaca acacggcaac cccgtaatcg aatcggagga aagaaaatgc
     4141 gttacgtcat cattgtattg tggtcgctga tctggtcggt gacttcggct actgcccagg
     4201 tcagcatcgg tatcggactt cccaacgtaa gcatcggcat caacctgccg ttgtatccgg
     4261 aactcgtgcc ggtgccgggc tacccggttt actacgcgcc gcgagtgagt tcgaactatt
     4321 tcttctacga tggcatgtac tgggtctacc a
//
LOCUS       DBNGSG010000418         1565 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2343752, whole genome shotgun sequence.
ACCESSION   DBNGSG010000418 DBNGSG010000000
VERSION     DBNGSG010000418.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2343752"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="AC158Q_10920"
     CDS             <1..904
                     /locus_tag="AC158Q_10920"
                     /inference="COORDINATES: protein motif:HMM:NF013465.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HIR3340328.1"
                     /translation="AIASGDENPHYVQPKPSFVPLLASADVFVTTGLDLELWVPALLD
                     KAGNPKVSEGGAGYVAAYAGLPLLDVPTNLSRSQGDIHVYGNPHIWTDPLNAALIARN
                     ILTALKRVAPADAAYFTEREQAFEARLYRALFGEELVRLLGGDALARLDRDGKLFDFL
                     RAQQYQGAPLIGRLGGWLQQALPFRGRTVACYHKEWDYFSREFQVPCFDYIEPKPGIP
                     PTPRHVQEMITAMRENKTQVLLSTNYYDRRQIQEVAQRTGATAVIVPSNTGPRTPTYI
                     DLMNLWVSELARAFAATPGAAGQP"
     gene            923..>1565
                     /locus_tag="AC158Q_10925"
     CDS             923..>1565
                     /locus_tag="AC158Q_10925"
                     /inference="COORDINATES: protein motif:HMM:NF013143.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HIR3340329.1"
                     /translation="MLELMLPPFVACMIMVAMHSYLGLHVIAREVIFVDLSLAQMAAL
                     GSVVALLVGVAPDSRTAYFFALGFTAFGAFLFALTRTSGKGRVPQEAIIGIVYVVASA
                     AAILVADKAPRGADAIKDILVGSLIWVTWPTIEWKLALPYAVLGVFHYALRKRFLMIS
                     FHPEEAERSGWKIRWWDFWFYLSFGIVITFSVPIGGVLMVFTFLVVPAAIAFLF"
BASE COUNT          224 a          528 c          523 g          290 t
ORIGIN      
        1 cgcgatcgcg tcgggtgacg agaaccccca ctacgtccag ccgaagccca gtttcgtgcc
       61 gctgctggcc tccgccgacg tcttcgtcac gacggggctc gacctcgagc tgtgggtgcc
      121 ggcgctgctc gacaaggccg ggaacccgaa agtctccgaa ggcggcgccg gatacgtggc
      181 cgcgtatgcg ggcctgccgt tgctcgacgt gccgaccaac ctctcccgct ctcaaggcga
      241 catccacgtc tacggcaatc cgcacatctg gaccgatccg ttgaatgcgg ccctgatcgc
      301 gcgcaatatt ctcacggccc tgaagcgggt cgcgcccgcg gacgcggcgt acttcaccga
      361 gcgcgagcag gcgttcgagg cgcggctgta ccgcgccctg ttcggggagg agctggtgcg
      421 gctgctcggc ggcgacgccc tcgccaggct cgaccgcgac ggcaagctgt tcgacttcct
      481 ccgcgcgcag caatatcagg gcgcgccgct catcgggcgg ctcggcggct ggctgcagca
      541 ggcgttgccg tttcgcgggc ggacggtggc gtgctaccac aaggagtggg actacttttc
      601 ccgggagttc caggtcccgt gtttcgatta catcgagccg aagccgggca ttccgccgac
      661 gccgcgccac gtccaggaga tgatcacggc gatgcgggag aacaagaccc aggtcctgct
      721 gtcgaccaat tactacgacc gccggcagat ccaggaggtc gcgcagcgca ccggcgcgac
      781 cgcggtgatc gtgccctcga acaccgggcc gcggacaccg acctacatcg acctgatgaa
      841 tctctgggtc tcggagctgg cgcgcgcgtt cgccgccacg cccggggccg ccggccaacc
      901 atgaacgggg gagacgcgca ccgtgcttga gctgatgctg cccccgttcg tcgcctgcat
      961 gatcatggtg gcgatgcact cctacctcgg cctgcacgtc atcgcgcgcg aggtgatttt
     1021 cgtcgatctc tcgctcgcgc agatggcggc gctcggcagc gtcgtggcgt tgctggtcgg
     1081 ggtcgccccc gattcgcgca cggcgtactt cttcgccctg ggcttcacgg cgttcggggc
     1141 gtttctcttc gccctgacgc gcacgtccgg gaaaggccgc gtgccgcagg aagcgatcat
     1201 cggcatcgtg tacgtggtgg cgtcggcggc ggccatcctg gtggcggaca aggccccgcg
     1261 cggcgccgac gccatcaagg acatcctggt cggcagtctg atctgggtga cgtggccgac
     1321 catcgaatgg aagctggccc tgccctacgc cgtgctcggg gtgtttcact acgcgctgcg
     1381 gaaacggttc ctcatgatct cgttccaccc ggaggaggcc gagcgctccg gctggaaaat
     1441 ccgctggtgg gatttctggt tttacctgtc gttcgggatc gtgatcacct tctcggttcc
     1501 catcggcggt gtgttgatgg tcttcacctt tctcgtggtg ccggcggcga tcgcgttcct
     1561 gttca
//
LOCUS       DBNGSG010000419         6169 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1664089, whole genome shotgun sequence.
ACCESSION   DBNGSG010000419 DBNGSG010000000
VERSION     DBNGSG010000419.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6169)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6169)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6169
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1664089"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="AC158Q_10930"
     CDS             <1..210
                     /locus_tag="AC158Q_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HIR3340330.1"
                     /translation="YAIEDVCTHDGGELAGGTLIGDEIECVRHGARFCIRTGAVMAPP
                     AYEPVAKFPVRVENGEIQVRDDRWD"
     gene            402..1460
                     /locus_tag="AC158Q_10935"
     CDS             402..1460
                     /locus_tag="AC158Q_10935"
                     /inference="COORDINATES: protein motif:HMM:NF016214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HIR3340331.1"
                     /translation="MDTFTHALSGALLARATAPAQPDGETLPLRRRLLIGFVAAAAPD
                     LDFVFGFLGPIEYLYYHRGITHSLILLPLWAYLLAKLCAVTWRRDRPWHAYFGIIAMG
                     IGIHIAGDWITSFGTIVFAPLSDARYGIGTTFIIDLWFTGIIAAGLLAAWIWRASPVP
                     AIAGLAALCGYIAFQGVQQQRAIEWGETYAKAAGLQPAQVTAQPRPVSPFNWLVIARS
                     GELVRYSFVNLVRREPLRLAPDAGRIARLDAAYLPLGQAQWVHGTLFGASDTERVIAR
                     EAWSHPQFEFFRWFADEPMLARIEMGNPSTCAWFQDLRFFTPGRSGWPFRYGMCREAG
                     GPWQLFQLDGLGRRVPVD"
     gene            1536..2357
                     /locus_tag="AC158Q_10940"
     CDS             1536..2357
                     /locus_tag="AC158Q_10940"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR3340332.1"
                     /translation="MGEPSYIPRGWLVACALALASCQSVQTTQPGAVGVDRNQRMALS
                     SAEVDKAAAQAYRQTLSEASKKGLLNRNAAQVARVRAIAQRLIPATASFRADAPRWAW
                     EVNVISSKDVNAWCMPGGKIAVYTGLIDQLKVTDDELAAVMGHEIAHALREHGRERAS
                     QQIAQQTVIGIGAALLGIGDAGASLVNVVADVTVGLPYSRGFEVEADRIGVELAARGG
                     YDPRAAVTLWQKMGKAGGSAPPEFLSTHPAPENRASDLTAYAARVMPLYEAARRR"
     gene            2436..3362
                     /locus_tag="AC158Q_10945"
     CDS             2436..3362
                     /locus_tag="AC158Q_10945"
                     /inference="COORDINATES: protein motif:HMM:NF012607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HIR3340333.1"
                     /translation="MIAVPDDFPSVFEGSEAHERAKALGSLRLCTGRGAEDEAELARR
                     IGSARVAVNIRAYSRFTESVFRSCPDLRMISIWGVGTDNVDLAAAARHGITVCNTPGA
                     NAYAVAEHAIALMLAVARRIPQIDREMRSGGWPRDMLTQLYGKSIGVFGTGSIGTRTA
                     QLARGLGMEVRAWSARRDTAAAKDDILRHADFITLHVRLTPETRNFIGRRELELMKPG
                     AILVNTGRGALIERDALLSALTAGRIAGAGLDVFHDEPIPPGDPLLGCANTVLSPHNA
                     GQTPEVRRDGLLRAIENVENFLAGKPTNVVCT"
     gene            3748..4032
                     /locus_tag="AC158Q_10950"
     CDS             3748..4032
                     /locus_tag="AC158Q_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340334.1"
                     /translation="MLMAPVAVGAQQQVRERAQVQSRVQERAGQREIIPGSELMTSQE
                     REAYRRRYAAAKTDEERAKVRAEHVKAMEERARLRGLRLADPVVPKGQAK"
     gene            4029..4313
                     /locus_tag="AC158Q_10955"
     CDS             4029..4313
                     /locus_tag="AC158Q_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3340335.1"
                     /translation="MKTSRVLLAICAALALAGPAAGAGDPVRGAKLHDDCLGCHGTEL
                     YVPPRAKVKTLAALKKETEKWNDRMNPKFTKQEIDDLVAYLNRDFYKFTK"
     gene            complement(4396..5226)
                     /gene="serB"
                     /locus_tag="AC158Q_10960"
     CDS             complement(4396..5226)
                     /gene="serB"
                     /locus_tag="AC158Q_10960"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HIR3340336.1"
                     /translation="MPLIIQGPNVETTVLKEVAKLSGADGIEQIAPNVFRLRNASPAD
                     ALADLCARSRLDFAFVPEERRLADFRLLVTDMDSTLITIETIDELASLVGKKDQVSEI
                     TTQAMRGEIPYDESLRRRVAMLKGLDESALASLYRDRVKLSPGADKLIAAAKKAGIKT
                     LLVSGGFTHVTERLKAQLKLDYTRANTLKVAGGKLTGEVEGVIVNADVKRDALIAVRD
                     ELKIGKQHIIAVGDGANDLKFMAEAGVSVAYHAKPVVAREAMHTIHYAGLDGVLRLFN
                     "
     gene            complement(5321..5902)
                     /locus_tag="AC158Q_10965"
     CDS             complement(5321..5902)
                     /locus_tag="AC158Q_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340337.1"
                     /translation="MMAVRNMNPAALRAAILAAELLVFFAVTAFMTYFISYAGALHPE
                     PGDAPPAHFPVIAYDGDRKRPEPKGYRVMPWSEWERTAQQRPGASLLLPERNARMPIG
                     ETEVSFTVTADNESRQSVDLTWRTPGGEQQVRYDAQARSIEPRYYRAVSSDTFLIAAG
                     AGFIAGLFVGRVLRRRWLAQPGYFVTTSSQGKN"
BASE COUNT         1071 a         1934 c         2066 g         1098 t
ORIGIN      
        1 tacgcgatcg aggacgtgtg cacccacgac gggggcgagc tggccggagg caccctcatc
       61 ggagacgaga tcgaatgcgt gcgccacggc gcgcgcttct gcatccgcac cggcgcggtc
      121 atggcgccgc cggcctacga gccggtcgcg aaattcccgg tgcgcgtcga gaacggcgag
      181 atccaggtgc gcgacgaccg gtgggattga aaacagaagc ataaaatagc cgccaagacg
      241 ccaagaacgc caagaaaatc catacggaaa taaatacaga gaactgaaat caaataagca
      301 gccgatatca tcaacagacc taagacacac gcctttcctg tttcactatt attgatcttc
      361 ttggcggcct tggcgtcttg gcggctaatt agaggttttg attggacaca tttacgcatg
      421 cgctgtccgg cgcgctgctg gcgcgggcga cggcgccggc ccagcctgac ggggaaacac
      481 tgccgctgcg gcggcgcctg ttgatcggct tcgtcgctgc ggccgcgccc gatctcgatt
      541 tcgtcttcgg cttcctcggg ccgatcgagt acctgtatta ccaccgcggg atcacgcact
      601 cgctgatcct gctgccgctg tgggcgtatc tgctggcgaa actgtgcgcg gtgacatggc
      661 gccgggaccg gccgtggcac gcctatttcg gcatcatcgc gatgggcatc ggcatccaca
      721 tcgcgggcga ctggatcacc tcgttcggca ccatcgtgtt cgcgccgctc tccgacgccc
      781 gctacggcat cggcacgacc ttcatcatcg atctctggtt caccggcatt atcgccgcgg
      841 gattgctggc ggcgtggatc tggcgcgcgt cgccggtgcc cgcaatcgcg ggcctggccg
      901 ccctgtgcgg atacatcgcg ttccagggcg tgcagcagca gcgcgccatc gagtgggggg
      961 aaacgtacgc gaaggccgcc gggctgcagc cggcgcaggt cacggcgcag ccgcgcccgg
     1021 tctcgccgtt caactggctg gtgatcgcga gaagcggcga actggtgcgc tacagcttcg
     1081 tcaacctggt tcggcgcgag ccgttgcggc tcgcgccgga tgccggccgc atcgcgcgcc
     1141 tcgacgcggc ctacctgccg cttgggcagg cgcagtgggt gcatgggacg ctcttcggcg
     1201 cgtcggacac cgagcgcgtg atcgcgcgcg aggcgtggtc gcacccgcaa ttcgagttct
     1261 tccgctggtt tgccgacgaa cccatgctcg cgcgtatcga gatgggtaat ccctcgactt
     1321 gcgcgtggtt ccaggacctg cggttcttca ccccgggccg cagcgggtgg ccgttccgct
     1381 acggcatgtg ccgtgaagcc ggcgggccct ggcagctttt ccagctggac ggactgggca
     1441 ggcgcgttcc ggtcgactga gaactaaccc cggagattac aatccattgc atgaagggcc
     1501 caggcgggaa cgggccggaa gcatcaggag gcggcatggg cgaaccatct tacattccga
     1561 ggggctggct ggtggcgtgc gcgctggccc tggcgtcctg ccagtcggtg cagaccacgc
     1621 agccgggtgc cgtcggcgtg gaccgcaacc agcggatggc gctgtcctcg gcggaagtcg
     1681 acaaggccgc cgcccaggct taccggcaga cactgagcga ggcctcaaaa aaggggctcc
     1741 tcaatcgcaa cgcggcgcag gttgcccgcg tacgcgcgat tgcgcaacgt ctcatacccg
     1801 caaccgcttc gtttcgtgcc gacgccccgc ggtgggcctg ggaggtcaac gtcatcagct
     1861 ccaaggatgt gaatgcctgg tgcatgcccg gcggcaagat cgccgtttac accgggctga
     1921 tcgaccagct caaggtcacc gacgacgagc tcgctgcggt gatggggcac gagattgccc
     1981 atgccctgcg cgagcacggc cgcgagcgcg cctcgcagca gatcgcgcag caaacggtga
     2041 tcggcatcgg ggcggcgctg ctcggcatcg gcgatgccgg cgccagcctg gtgaacgtcg
     2101 tggcggacgt cacggtcggc ttgccgtatt cgcgcggatt cgaggtcgaa gccgaccgca
     2161 tcggcgtcga actcgccgcg cgcgggggat acgatccgcg cgccgcggtc acgttgtggc
     2221 agaagatggg aaaggcgggc ggctcggcac cgccggagtt tctctcgacg caccccgcac
     2281 ccgagaatcg cgccagcgat ttgaccgctt acgcagcgcg cgtcatgccg ctctacgaag
     2341 ctgcacgccg gcgctgatgg cgtggacggc gtgacgagtg acgaggctcc cgcgagaggg
     2401 ccgcggacat ccgtcactaa tcacctatca cttgaatgat tgccgttccc gacgactttc
     2461 cgtccgtctt tgaaggctcg gaggcccacg agcgcgcaaa ggcgctgggc agcctgcgtt
     2521 tatgtaccgg acgcggcgcg gaggacgagg cggagctggc gcgccggatc gggagcgcac
     2581 gggtagcggt caacatccgc gcctattccc gcttcacgga atccgtcttc cgctcgtgcc
     2641 cggacctcag gatgatctcg atctggggcg tgggaacgga caacgtcgac cttgccgcgg
     2701 cggcccgcca cggcatcacc gtttgcaaca cgccgggtgc caacgcttac gcggtggccg
     2761 agcatgcaat cgccctgatg ctcgcggtgg cgcgcaggat cccgcagata gaccgcgaga
     2821 tgaggagcgg cgggtggccg cgcgatatgc tcacccagct ctatggcaag tcgattgggg
     2881 tatttggcac cggcagcatc ggcactcgga cggcccagct cgcgcgcggg ctcggcatgg
     2941 aagtgcgggc atggagcgcg cggcgcgata cggccgcggc aaaggacgac atcctgcggc
     3001 atgccgattt catcacgctc cacgtgcggc tcacgccgga gacgcgtaac ttcatcggcc
     3061 ggcgggaact ggaattgatg aagcccggcg cgatcctggt caataccggc cgcggcgcgc
     3121 tcatcgagcg cgatgcgctc ctttctgcac tgacggcagg cagaattgcg ggtgccggcc
     3181 tcgacgtatt tcacgacgag ccgatcccgc ccggggatcc gctgctgggc tgcgccaaca
     3241 ccgtgctctc accccataac gcgggacaga caccggaagt gaggcgcgac ggcctgctgc
     3301 gcgcgatcga gaacgtcgaa aatttcctgg ccggaaagcc gaccaatgtc gtttgcacct
     3361 agcgctatat ccgggtggac ggcgtcgatc cctctctcac cgcgcgggtg cgcgccggcg
     3421 ccgcgctaag cgctcggcct ggccctgcgc aatcgggccc cggagggaag cttccggaaa
     3481 tttgatctgc atcaagctcc gcgcgtggat tgcatactag attgctagtc gtcatctcaa
     3541 aaaccgtttt agccacggaa acacacggaa tcacacagaa attcctgaat gattacggag
     3601 actctaatgg gtcgggttat tgtccgtgtc attctgtgtg attccgtggc tattattgag
     3661 accggttcta gtgttttcta taccagcacg ggagttcgca tgggaacaat gcttcgggcg
     3721 gcagtttcat cggtgacagg ggtggtcatg ttgatggccc ctgtggccgt gggcgcgcag
     3781 cagcaggtgc gcgagcgcgc gcaggtccag agccgcgtcc aggagcgcgc aggccagcgc
     3841 gagatcattc ccgggtccga actcatgacc tcgcaggagc gcgaggcata ccgccggcgc
     3901 tacgcggccg cgaagaccga cgaggaacgg gcgaaggttc gcgccgagca cgtgaaagcg
     3961 atggaagagc gcgcacgcct tcggggactg cggctcgcgg accccgtggt gccgaaaggc
     4021 caggccaaat gaaaacgagc cgggtgctgc ttgccatttg cgcagcgctg gcgctggccg
     4081 ggccggccgc aggcgccggt gacccggtca ggggcgcgaa gctgcatgat gactgcctgg
     4141 gctgccacgg caccgagctg tacgtgccgc cccgcgccaa ggtcaagacg ctcgcggcgt
     4201 tgaagaagga gacggaaaag tggaacgacc ggatgaaccc caagttcacg aagcaggaaa
     4261 ttgacgacct cgtcgcttat ctcaatcgcg atttctacaa gttcacgaaa tgagaaggcg
     4321 aagcggcgaa gcggtgatgc ggtgaggtag tgacgattca cccgtccctt catcgcctcg
     4381 ccactgcgcc aatcttcagt tgaacaggcg cagcacgccg tcgaggccgg cgtaatggat
     4441 cgtgtgcatg gcctcgcggg cgaccacggg cttggcgtgg taggcgaccg agacacccgc
     4501 ttctgccatg aacttgaggt cgttcgcgcc gtcgccgacg gcaatgatgt gctgtttccc
     4561 gattttcagc tcgtcgcgta ccgcgatcag cgcatcgcgc ttgacgtcgg cgttgacgat
     4621 gacgccctcg acctctcccg tcagttttcc acccgccacc ttgagcgtgt tcgcgcgcgt
     4681 gtagtcgagc ttgagctgcg ctttcaggcg ttccgtgacg tgggtgaacc cgccggaaac
     4741 gagcagggtc ttgatgcccg cttttttagc ggccgcgatc agcttgtcgg cgccaggcga
     4801 aagcttcacg cgatcgcggt agaggctcgc gagggcgctc tcatccagcc ccttcagcat
     4861 cgcgacgcgg cggcgcaggc tctcgtcgta ggggatctcg ccgcgcatcg cctgcgtggt
     4921 gatctccgac acctggtctt tcttgccgac gaggctcgcg agctcgtcga tggtttcgat
     4981 ggtgatcaga gtcgagtcca tatccgtcac cagcagccgg aaatcggcga ggcggcgctc
     5041 ttccggcacg aacgcgaagt cgagcctgct cctggcgcac aggtcggcga gagcgtcggc
     5101 gggggacgcg ttgcgcaggc ggaatacgtt gggcgcgatc tgctcgatgc cgtccgcgcc
     5161 ggagagcttg gcgacttcct tcagaacggt ggtctctacg ttggggcctt gtatgatcag
     5221 cggcatagtg gcttgtaata acggaaaagg gataaggggt tgaaccgcac gtttttcccg
     5281 gagttcaaat tttaattctt ttttcttggc gtcttggcgg ttaatttttg ccttgcgacg
     5341 aagtcgtaac aaagtaaccc ggttgcgcga gccagcgccg gcgcagcacc cggcccacga
     5401 agaggccggc gatgaacccg gccccggcgg cgatcaggaa agtatcggag ctgaccgcgc
     5461 ggtagtagcg cggttcgatc gagcgcgcct gggcatcgta acgcacctgc tgctccccgc
     5521 ccggcgttct ccaggtgaga tcgacgctct gccgtgattc attgtccgca gtcacggtga
     5581 acgacacttc cgtttctccg ataggcatgc gggcgttgcg ttcgggcagc agcaggctcg
     5641 cgcccgggcg ctgctgggcg gtgcgttccc actcgctcca gggcatgacc cggtagccct
     5701 tcggctcggg ccgcttccgg tctccgtcgt aggcaatgac cgggaagtgc gcgggcggcg
     5761 catcgccagg ctccgggtgc agcgctcccg cgtaggagat gaaataggtc ataaacgccg
     5821 tgacagcgaa gaacaccagc agttcggcgg cgagtatggc ggcccgcagc gcggcgggat
     5881 tcatgtttct gacggccatc acgggggctc gcggaggatt atagcgggtg cgcccggtgt
     5941 ctttcgagcg ttatggcgtt actgcgcatc gctcgaccct cacctattcc cccctcccgg
     6001 agggcggggg gaaaagtacg gtgcggaaat gtcgtttcat gcatgtccgc aaaagatcaa
     6061 ttggccagtt ccccgaacgc ctcgcggatg gccgcgacgc gcgccttgat atcgggcagc
     6121 gcgagctcga tgcgcagccg gtcctgcccc gcgaggcggt agtgcttct
//
LOCUS       DBNGSG010000420         2414 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4381272, whole genome shotgun sequence.
ACCESSION   DBNGSG010000420 DBNGSG010000000
VERSION     DBNGSG010000420.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4381272"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="AC158Q_10970"
     CDS             <1..786
                     /locus_tag="AC158Q_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR3340338.1"
                     /translation="QAREQVFYGEATAELARARQAALSERERLTRMMGLWGEDINFSL
                     PARLPDLPKSAREIADLEAQALKQRLDVQGAMQEAENVAASLGLTQATGFISVLEVGY
                     QRTSETGQPRKSGYEIEIRLPLFDWGGARTARAEHTYMQAVNRAADIAVRARSEVREG
                     YAAYRTAFDLARHYRDEIVPLRKRISEENLLRYNGMLVSVFELLADARQQIAAVNTYI
                     EALRDFWLAEATLQLALTGKSPGGITLSSSSSPYAASGNAAAH"
     gene            804..2174
                     /locus_tag="AC158Q_10975"
     CDS             804..2174
                     /locus_tag="AC158Q_10975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HIR3340339.1"
                     /translation="MTNRRQFLRTAGGALLGAAAVSRVAQGSVPEAPMEDKPDTRPPL
                     SPPNGRPYQPVVTLNGWTLPWRMNNGWKEFHLVAEPVRRQMAPGMTAHLWGYNGQSPG
                     PTIECVEGDKVRIFVTNKLPEHTTIHWHGILLPNGMDGVGGLTQPQIPVGKTFVYEFE
                     MKKSGTFMYHPHADEMVQMAMGMMGFIVVHPRNPGFMKVDRDFVFLINAYDIEPGAYT
                     PKIATMTDFNLWSWNSRVFPGIAPLAVRRGDRVRIRIGNLTMTAHPIHLHGHDFEVTG
                     TDGGWVPKTARWPDVTTDVAVGQMRAIEFVADAPGDWAFHCHKSHHTMNAMGHGVPTM
                     IGVDHRGIAQRIINLVPDYMVMGERGMADMGEMEMPLPDNTLPMMTGRGPFGPLEMGG
                     MFTVVKVREGLARNDYRDPGWYKHPQGTIAFEWKGTAPESKKVSQASATGRELRARKP
                     TGHGSH"
BASE COUNT          473 a          801 c          773 g          367 t
ORIGIN      
        1 caggcccgcg agcaggtttt ttacggcgag gcgaccgcgg agctcgcgcg cgcaagacag
       61 gccgccctct ccgagcgtga gcggttgacc cggatgatgg ggctgtgggg cgaggacatt
      121 aacttcagtc ttccggcccg gctccccgac ctgccgaagt cggcgcgtga gatcgccgat
      181 ctcgaggcgc aggccctgaa acaacgtctc gacgtgcagg gcgccatgca ggaagcggaa
      241 aatgtcgccg cctccctcgg tctcacccag gcgaccggct tcatcagcgt gctcgaagtg
      301 gggtaccagc gcaccagcga aaccggccag ccgcgcaagt cgggctacga gatcgaaatc
      361 cggctgccgc tcttcgactg gggcggcgcc cgcaccgcgc gcgcggagca cacctacatg
      421 caggctgtta accgggcggc cgacattgcc gtgcgtgccc gctcggaagt gcgcgagggt
      481 tacgccgcct accgcaccgc gttcgacctc gcgcggcact accgcgacga gatcgtgccg
      541 ctgaggaagc gcatctccga ggaaaacctg ctgcgctaca acggcatgct ggtgagcgtg
      601 ttcgagctgc tggccgacgc gcgccagcag attgcggccg tcaacactta catagaggcg
      661 cttcgggatt tctggctggc cgaggcgaca ttgcagcttg cgctcaccgg aaaatctcct
      721 ggcggcatca cactgtccag cagcagctcg ccctacgcgg ccagcggaaa cgcagccgca
      781 cattgaggcc ccgggagatt ttgatgacaa accgaagaca gtttctgcgg acagcgggcg
      841 gcgcgttgct tggcgccgcc gcggtgagca gagtcgccca gggctcggtg cccgaggcgc
      901 cgatggagga caagcccgat acgcggccgc cgctctcccc gcccaatggc cgtccgtacc
      961 agccggtggt cacgctgaac ggatggacgc tcccctggcg catgaacaac ggctggaaag
     1021 aattccacct ggtcgcggaa ccggtgcgcc ggcagatggc gcccggcatg accgcgcacc
     1081 tgtggggcta caacggccag tcccccgggc cgacgatcga gtgcgtcgag ggcgacaagg
     1141 tgcgcatctt cgtcaccaac aagctgccgg agcacaccac catccactgg cacggcatcc
     1201 tgctgcccaa cggcatggac ggcgtgggcg gcctgaccca gccgcagatc cccgtcggca
     1261 agaccttcgt ctacgagttc gagatgaaga agagcggcac cttcatgtac caccctcacg
     1321 ccgacgagat ggtgcagatg gcgatgggga tgatgggatt catcgtcgtg cacccgagga
     1381 acccgggctt catgaaagtg gatcgtgact tcgtgttcct catcaacgcc tacgacatcg
     1441 agcccggcgc ctatacgccg aagatcgcca ccatgacgga cttcaacctg tggtcgtgga
     1501 acagccgggt gttcccgggc atcgccccgc tcgccgtgcg ccggggcgac cgcgtgcgca
     1561 tccgcatcgg caatctcacc atgaccgctc accccattca cctccatggc cacgacttcg
     1621 aggtcaccgg caccgacggc ggctgggtgc cgaaaacggc gcgctggccg gacgtgacga
     1681 cggatgtcgc cgtcgggcag atgcgcgcga tcgagttcgt cgccgacgcg cctggcgact
     1741 gggcgtttca ctgccacaag tcgcaccaca ccatgaatgc aatgggccac ggcgtgccga
     1801 cgatgatcgg cgtggaccat cgcggcatcg cgcagagaat catcaacctc gtgcccgact
     1861 acatggtcat gggcgagcgc ggcatggccg acatgggcga gatggaaatg ccgctgccgg
     1921 acaacacgct gccgatgatg accggcagag ggcccttcgg cccgctcgag atgggcggca
     1981 tgttcacggt ggtcaaggtg cgcgaagggc tcgcgcgaaa cgactacagg gatccgggtt
     2041 ggtacaagca tccgcaggga accattgcgt tcgaatggaa aggcacggcg cccgaatcga
     2101 agaaagtctc ccaggcttcc gccacgggca gggagttgcg tgcgcgcaag ccgaccggcc
     2161 acggcagcca ttagcccgat gcacaacggc aacaccattt aacccgctat caccatgaaa
     2221 ggagatcgta ttgatacatc cgttactcgc ggcagccgct gcgttactcg ttgtttcccc
     2281 tgtcgccctt ggacacggcg agaatcctca ggcgcggaaa ttcagccccg cgcaactggc
     2341 cgaagaaaag ccgttcggca ggtccggaga cccgaagaaa gtcacgcgca ccgtcacctt
     2401 caccatgagc gaca
//
LOCUS       DBNGSG010000421         6616 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1155766, whole genome shotgun sequence.
ACCESSION   DBNGSG010000421 DBNGSG010000000
VERSION     DBNGSG010000421.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6616)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6616)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6616
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1155766"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="AC158Q_10980"
     CDS             <1..483
                     /locus_tag="AC158Q_10980"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF019121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR3340340.1"
                     /translation="RARARALRRRLAQPVLSEAFIPGRDLYVGLLGNGPRMLPPVELV
                     VRSRHRAAPRLATYRLKNDPRYRAKWKAHYRRARLDARTLRKLAEASARIFHALKLRD
                     YARLDFRLTDDGRFYFIEANPNPDLDPYALNRNGCFAGVPYQRLLSTIVESARKRKRE
                     "
     gene            complement(637..1290)
                     /locus_tag="AC158Q_10985"
     CDS             complement(637..1290)
                     /locus_tag="AC158Q_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340341.1"
                     /translation="MATELLDTPLFLAAIALGALGVLLFLAGILAFFRLEFAGSAYRI
                     VFGLLIISLGALAGTVAVGIQGYRVLTREDVAARIMVQPAGPQRFSATFKFPDGRQAT
                     YEIAGDEIYVDAHILKWNPHANVIGLHTGYELDRVGGRYHNIKDERAATRTMHSLGRE
                     RPVDLYAFSRRYTFLAPLLDAEYGSATFIPVTKPAEFELRVSTTGLLIREAKPAQGK"
     gene            complement(1466..1825)
                     /locus_tag="AC158Q_10990"
     CDS             complement(1466..1825)
                     /locus_tag="AC158Q_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013207189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HIR3340342.1"
                     /translation="MTRQKQRKTRREIWRDTATPAGVVRVNAEALAPYNSYGTPLFVQ
                     RGYYEDVRFTCSGCGSREVWRAAQQKWWYEVAKGNVCSGAKLCRACRRREQARRDEAR
                     RVHLEGIARRRDRGPGH"
     gene            complement(1919..2164)
                     /locus_tag="AC158Q_10995"
     CDS             complement(1919..2164)
                     /locus_tag="AC158Q_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340343.1"
                     /translation="MARKIELHLDALVAIALLIVAAVGFIAYQRHQYLDLLQDNIDRQ
                     WKQMALEFEVARLEAQLKKAASEATAASGAPQEQKKP"
     gene            complement(2197..2922)
                     /locus_tag="AC158Q_11000"
     CDS             complement(2197..2922)
                     /locus_tag="AC158Q_11000"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3340344.1"
                     /translation="MTSAHHNELGQPVGAALPGWKGVERPPRAPMAGRYCRVEPLDIA
                     RHAQDLWQAGALDAAGRNWTYSAAGPFASFDAYRQWMESSCLGEDPLYHAIVDAATGK
                     AAGVASYLRIDPKNGVIEVGHINYSPLLQRKRAATEAMYLMMKRVFELGYRRYEWKCD
                     SLNAPSRAAAQRLGFSYEGIFRQALVYKGRTRDTAWFAMIDAEWPELERAFLRWLDPA
                     NFDAQGNQRLRLADLTAPIRKAR"
     gene            complement(3140..4135)
                     /locus_tag="AC158Q_11005"
     CDS             complement(3140..4135)
                     /locus_tag="AC158Q_11005"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3340345.1"
                     /translation="MPSFDDPPVLTHPMMKHFFNLLPVLAAAALLLATGAARAQQTAN
                     DAERCASVSGSPDLAIQHCTRAIESRRYSGEELAKLHYNRGIEWAAKGDYDRAITDYD
                     TAIRINPGNGDAFYNRASAWGNKGDHDRAIADYDAAIRLNPKDAASYNGRAYERVAKG
                     DYARAIADYDTAIQLDPKLAVALLARARVRYYTGDFLRAVSDLEQSLKIDHNEYTSLW
                     LYLARKGTGAANAEELLDSETRASRSSGWPGPVIVLYLGRTDAESVFAAATDSDARKQ
                     REQRCEANFYLAHWHLLRGDRERALPLLKEAQSGCPRDFLEHEGAVAELRRLQKP"
     gene            4238..4765
                     /locus_tag="AC158Q_11010"
     CDS             4238..4765
                     /locus_tag="AC158Q_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340346.1"
                     /translation="MRPLLRCLLAPAALLLPLAGAAADGAAAFGNLVQSRYVHCAFYR
                     AYEVDRTTGDLLLVEGRSNSLTHFQAIRDGNARQISTRVAGARNVRVMNSGKYLHFVD
                     EVAGMYVLTTIYDCLDRDPKSGTCISYGATQSRHFDPRVLTDPDAVYEALKEISDPGF
                     CDHSFIGMQEASRGN"
     gene            complement(4933..5733)
                     /locus_tag="AC158Q_11015"
     CDS             complement(4933..5733)
                     /locus_tag="AC158Q_11015"
                     /inference="COORDINATES: protein motif:HMM:NF015377.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HIR3340347.1"
                     /translation="MNLLFFPVVALAALLFAVGYRTGMRPGASAVNGWWYAAAAVCAL
                     PGVLFAVYYLHLFDDAVWFYRFRSWPFTELSASGAGLLAGLLQAWTVRKGLRVRPLLP
                     GLLLAGLFIPYMKSVVAPLDLTTLAAECRDGVCLQSTPSTCGPSSVATLLRMFNIEET
                     EKTLAREAYTSSGGTENWYLARALRRRGFPVEYVVADRPVTRLPYPSVAGVVLPGGAG
                     HYISVLGETDSAWVVGDPMYGRVTVPKSRPGAAYNFTGFFMVVGKPQA"
     gene            complement(5786..>6616)
                     /locus_tag="AC158Q_11020"
     CDS             complement(5786..>6616)
                     /locus_tag="AC158Q_11020"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3340348.1"
                     /translation="LIIEILGREKIITQPMIVSSRTGGAGAVAFNYVKSKRGEPHTVL
                     TIATGSFLTATSRPDLDLGPEHFTPLAFFARDPQGVMVLASTPWRTFKDLIDRAKREP
                     EAIVCAVTSATGTGRQTLWRIERATGAKFKFVTFKAGSEAVLSVLGGHVPFTTENVSE
                     AWAHIEAKKMRVLAVTTEKRMPALPAVPTLTELGYDVKIGTGRGFGMPAGVPKEAVAA
                     MEAALKKAHDSPMWNDYATKNMYEDRWMGSAEFTAYLTEQRLAQKEFVDSIGLSKKKP
                     "
BASE COUNT         1130 a         2134 c         2163 g         1189 t
ORIGIN      
        1 cgcgcgcggg caagggcatt gcggcggcgg ctcgcgcagc cggtgctgag cgaggcgttc
       61 atccccggcc gggacctcta cgtcgggctg ctcggcaacg ggccgcggat gctgccgccg
      121 gtggaactgg tggtgcgcag ccgccatcgc gccgcgccgc ggctggcgac ctaccggctg
      181 aaaaacgatc cccgttaccg ggcgaagtgg aaagcgcatt accgccgcgc ccgcctcgat
      241 gcgcggaccc tgcgcaagct cgcggaggcc agcgcgcgta tttttcacgc gctgaagctg
      301 agggactatg cccgtctcga tttccgcctg acggacgacg ggcggtttta tttcatcgag
      361 gccaacccca accccgacct ggacccttac gcgctgaacc gcaacggctg cttcgcgggc
      421 gtgccgtacc agcggctgct ctcgactatc gtggagtccg cccgcaagcg gaagcgcgaa
      481 tagacaggat ttacagaatg attcaaaaac ccgtcaaaag caccaataga caggatttac
      541 aagattaaca agatcagatc tgaaaacaaa accacactct gattcagagt cccgtgattt
      601 tgattttaac aatcttgtaa atcttgttaa tcctgtctat tttccctgag cgggtttggc
      661 ttcgcggatg agcaagcccg tggtggagac gcgcagctcg aactcggcgg gcttggtaac
      721 cgggatgaac gttgccgagc cgtactccgc gtcgaggagc ggcgcgagaa aggtgtagcg
      781 cctgctgaac gcgtacaggt ccacgggccg ctcgcggccg agcgagtgca tggtccgcgt
      841 cgccgcgcgc tcatccttga tgttgtggta gcgccctccc acgcggtcga gctcgtaacc
      901 ggtatgcagc ccgatcacgt tggcgtgggg attccacttg aggatgtgcg cgtccacgta
      961 gatctcgtct cccgcgatct cgtacgtcgc ctggcggccg tccgggaact tgaacgtcgc
     1021 gctgaagcgc tgcgggcccg cgggttgcac catgattcgt gccgccacgt cctcgcgcgt
     1081 caatacccgg tagccctgga tgccgacggc gaccgtgccc gcgagagcgc cgagcgagat
     1141 gatcagcagg ccgaagacga tgcggtacgc ggaacccgcg aattccagcc tgaagaacgc
     1201 gagaatcccg gccaggaaca gcagcacgcc gagcgcgccg agcgcgatgg cggcaaggaa
     1261 cagcggcgta tcgagtagct cagttgccat ggcggctttc ccgggttgtg tctaacagta
     1321 agccagaata ggaaggttgg gcgattttaa caagtgcaaa aacgcacacc cggtgccgaa
     1381 cccgctccgg atcgacgcgc cggcgagacc cgcagtccgg gtgccgtgca tcgccggtgg
     1441 cggccaccgc gtggaagcgc cggagtcagt ggccggggcc gcgatcgcgc cggcgcgcga
     1501 tcccttcgag atgcacgcgc cgcgcttcat cgcggcgcgc ctgctcgcgg cggcggcagg
     1561 cccggcagag cttcgcgccg gaacacacgt tgcccttcgc cacctcgtac caccattttt
     1621 gctgggccgc acgccagacc tcccgcgaac cgcagccgct gcaggtgaac cggacatcct
     1681 cataatagcc gcgttgcacg aacagcggcg tgccgtagct attatagggc gcgagcgctt
     1741 ccgcgttcac ccgcaccacg cccgcgggcg tcgccgtgtc gcgccagatt tcgcgcctgg
     1801 tcttgcgttg cttctgccgt gtcatgtgaa gcgcccggga cgggttgtcg aggttcagcg
     1861 gccgcgcagc atccaacccg ttgtcaggtt atccgttttc aggcacgccg acggcgcctt
     1921 acggtttctt ttgctcttgc ggggcgccag acgccgccgt tgcttccgac gcggctttct
     1981 tcagctgtgc ttccaggcgt gccacttcga actccagcgc catctgcttc cactgccgat
     2041 cgatattgtc ctggagcaag tccaggtact ggtgcctctg gtacgcgatg aagccgacag
     2101 cggcaacaat cagcagcgcg atcgctacaa gcgcgtccag gtgcaattcg atcttcctgg
     2161 ccatgcggcg gtctccttgt tgattgaacc ccggattcaa cgcgccttgc ggatgggggc
     2221 ggtcaggtcg gccaggcgca gccgctggtt tccctgtgcg tcgaaattcg ccgggtcgag
     2281 ccagcgcaga aaagcgcgtt ccagttccgg ccattcggcg tcgatcatcg cgaaccaggc
     2341 ggtatcgcgg gtccggccct tgtagacgag ggcctggcgg aagatgccct cgtacgaaaa
     2401 tcccagacgc tgcgcggcgg cgcgggatgg cgcattgagc gagtcgcact tccattcgta
     2461 gcgccggtaa ccaagctcga acacccgctt catcatgagg tacatcgcct cggtcgccgc
     2521 ccgcttgcgc tgcagcagcg gcgagtagtt gatgtgcccg acctcgatca cgccgttttt
     2581 cggatcgatg cgcaggtagc tcgccacgcc ggccgccttg ccggtcgcgg catccacgat
     2641 ggcgtgatac agcgggtcct cgccgagaca ggacgattcc atccactggc ggtacgcatc
     2701 gaatgaggcg aacgggccgg ctgccgaata ggtccagttg cggcccgcgg catccagcgc
     2761 gcccgcctgc cagaggtctt gcgcgtggcg ggcgatgtcg agcggctcca cgcggcagta
     2821 gcgcccggcc atcggcgcgc ggggagggcg ctcgacgccc ttccagcccg gcagcgcggc
     2881 gccgaccggc tggcccagct cattgtgatg tgcggaagtc aaaggtgagt ggacaggatt
     2941 cacaaaccaa ttcgaaaacc cgtcaaaagc atcaatagac aggattaaca ggatttacag
     3001 gattagatca aaaggaagac tgcatcatga ttccacgctt aggggttcat ttttctttct
     3061 taatcttgtt aatcttgtaa atcctgtcca ttttcgatct tgacgggttt ttgaggtctt
     3121 tccgtcgatc ctgtctattt cagggttttt gcaggcggcg cagctccgcc accgcgccct
     3181 cgtgctcgag aaaatcccgt gggcagccgc tttgcgcttc cttcagcagc ggcagcgcgc
     3241 gctcgcggtc tccgcgcagg aggtgccagt gggcgaggta gaaattcgcc tcgcagcgct
     3301 gttcgcgctg tttcctggca tcgctgtccg tggcggcggc gaaaacggat tcggcgtccg
     3361 tgcggcccag gtacagcacg atcaccggcc cgggccagcc gctgctgcgg ctggcgcggg
     3421 tctcgctatc gagcaattcc tcggcattgg ccgcaccggt gcccttgcgg gcgaggtaca
     3481 gccacagcga ggtgtattcg ttgtggtcga tcttgagcga ttgctcgaga tcggataccg
     3541 cgcgcaggaa atcgcccgtg tagtaccgca cccgcgcccg ggcaagcagc gcgacggcca
     3601 gtttcgggtc gagctgaatg gcggtgtcgt aatccgcgat cgcgcgcgcg taatcgccct
     3661 tggccacccg ctcgtaggcg cggccgttgt acgaggccgc gtccttcggg ttcaggcgga
     3721 tggcggcgtc gtaatcggca atggcacggt cgtgatctcc cttgttgccc caggcgctgg
     3781 cccggttgta gaaggcgtca ccgttgcccg ggttgatgcg gatggcggtg tcgtaatccg
     3841 tgattgcccg gtcgtaatct cccttggccg cccactcgat cccgcggttg tagtgcagct
     3901 tggcgagttc ctcgccggag tagcggcggg actcgatcgc ccgggtgcaa tgctggatcg
     3961 ccaggtccgg gctgcccgaa acgctcgcgc agcgctcggc gtcgttggcg gtctgttgcg
     4021 cccgcgccgc gccggttgcg agcaaaagcg cagccgcggc cagtaccgga agtaagttga
     4081 agaagtgctt catcatgggg tgggtcagga ccggtggatc gtcgaaggac ggcattgtac
     4141 ctgcattctc aagcccttga tcctccggca tgaatggccc cgtggagcgg cccggccggg
     4201 tggacagggc cgcgacactg gtggcacact gcgctcgatg agaccgctcc tgcggtgctt
     4261 gcttgccccg gcggctttgc tcttgccgct tgcgggcgcg gcggcggatg gcgccgcggc
     4321 tttcggcaac ctggtccagt cgcgctacgt tcattgcgcg ttctacaggg cctacgaagt
     4381 ggaccgcacg accggcgacc tgctgctggt cgaagggcgc tcgaattcgc tcacccactt
     4441 ccaggccata cgcgacggca acgcccgcca gatatctacg cgtgtcgcgg gcgcccgcaa
     4501 cgtgagggtg atgaacagcg gcaagtacct gcacttcgtg gacgaggtcg cgggcatgta
     4561 cgtgctcacc acgatttacg actgcctcga ccgcgacccg aagagcggca cctgcatctc
     4621 ctacggcgcg acgcagtcgc gccacttcga tcccagggtg ctgaccgatc ccgatgcggt
     4681 gtacgaggcg ctgaaagaga tttcggaccc cggtttctgc gaccacagct tcatcggcat
     4741 gcaggaggcc tcacgcggaa attagccgcc aagtctgtcc tgagcaggtc gaagggacgc
     4801 caagaaaagc agaacaatat ttgaaccgcc agggccgcca aggaaaagca gaatatagtc
     4861 acagggaaca ccgggaaagc ttgctagggc ggatccggac cgcaactgcg ctgctcccgc
     4921 tccggccagg tgtcaggcct gcggtttgcc gacgaccatg aagaagccgg tgaaattgta
     4981 ggcggcgccg gggcgcgact tcgggacggt cactcgtccg tacatcgggt cgcccaccac
     5041 ccaggccgag tcggtttccc cgagaacgga gatgtaatgg ccggcgcctc ccggcagcac
     5101 gacgcccgcc actgacgggt aaggcagtct tgtgaccggc ctgtcggcga caacgtactc
     5161 caccgggaat ccccgccggc gcagcgctcg cgcgagatac cagttctccg tgccgccgga
     5221 ggaggtgtag gcttcccgcg ccagcgtctt ctcggtttcc tcgatgttga acattctcaa
     5281 taaggtcgcc acgctcgagg gaccgcaggt cgacggcgtg ctttgcaggc acacgccgtc
     5341 ccggcactcg gcggccagcg tggtcaggtc gaggggagcg accacggact tcatgtaggg
     5401 aatgaacagc ccggccagca gcaaccccgg cagcaggggc cgcacgcgca gccctttcct
     5461 gacggtccat gcctggagca gcccggccag cagcccggcg ccggaggcgc tcaactcggt
     5521 gaacggccat gacctgaacc ggtagaacca cacggcgtcg tcgaacaggt ggaggtaata
     5581 gaccgcgaac agcaccccgg gcagggcgca cacggccgcg gcggcgtacc accacccgtt
     5641 gaccgcggat gcccccggcc gcatgcccgt ccggtagccc acggcgaaga gcagcgccgc
     5701 gagcgccacg acggggaaga acagcaggtt catcgccacg cgcctggccg ggcggcaatc
     5761 agtcgacggc tgtcaccgcg cctcgtcagg gtttcttttt cgaaagcccg atggaatcca
     5821 cgaactcctt ctgcgccagg cgctgctcgg tgagatacgc cgtgaactcg gcgctgccca
     5881 tccagcggtc ctcgtacatg ttcttcgttg cgtagtcgtt ccacatcggg ctgtcgtgcg
     5941 cttttttaag ggccgcttcc atggcggcca cggcttcctt cgggacgccc gcgggcatgc
     6001 cgaatccgcg cccggtgccg atcttgacgt cgtagccgag ctcggtgagc gtcggcacgg
     6061 cgggtagcgc cggcatgcgc ttctcggtgg tcaccgcgag cacccgcatt ttcttcgcct
     6121 cgatgtgcgc ccaggcctcg ctcacgttct cggtggtgaa cgggacgtgg ccgcccagca
     6181 ccgagagcac cgcctcggag ccggccttga acgtgacgaa cttgaatttt gccccggtcg
     6241 cgcgctcgat gcgccacagg gtctgccggc cggtgcccgt ggccgaggtc acggcgcaga
     6301 cgatcgcctc gggctcgcgc ttggcgcgat cgatcaggtc cttgaacgtc ctccatgggg
     6361 tcgaggcgag gaccatgacg ccctgcgggt cgcgggcgaa gaacgcgagc ggcgtgaaat
     6421 gctccggccc gaggtcgagg tcggggcgcg aggtggccgt gaggaacgag ccggtcgcga
     6481 tggtcagcac cgtgtgcggc tcgccgcgct tgctcttcac gtaattgaac gcgaccgcgc
     6541 cggcgccgcc ggtgcggctc gagacgatca tcggttgcgt gatgattttc tcgcggccga
     6601 ggatttcgat gatcag
//
LOCUS       DBNGSG010000422         3275 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_477149, whole genome shotgun sequence.
ACCESSION   DBNGSG010000422 DBNGSG010000000
VERSION     DBNGSG010000422.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_477149"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..1102
                     /locus_tag="AC158Q_11025"
     CDS             137..1102
                     /locus_tag="AC158Q_11025"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA dioxygenase family protein"
                     /protein_id="HIR3340349.1"
                     /translation="MAATSLANRGAQRARFEIVPTPAALGAEVRGFDLKSLDDATFKE
                     LHRAWLDHVLLVFRGQSLAAEDLVRLVKRFGTPVTSSGLHKRELKERAGNELFNLPPE
                     VTVVSNVKQEGKSVGILGDGEVVWHSDFSFKERPTAARMLLAMEVPPQELGGFTYFLN
                     CYAAYDALSAAMKKRISGLTIKQADIVDTAMKVRPGMSLDMDYRSVPGPSHPVISTHP
                     ETGHNMLFLGRRHGSYVNGFSLEDSEALLNELWAHCTQPRFWYEHRWSVGDVVVWDNR
                     ATLHRRDPFESASRRVLYAAQVEGHKPYEAPDALARAPHPRAAQA"
     gene            complement(1099..1944)
                     /locus_tag="AC158Q_11030"
     CDS             complement(1099..1944)
                     /locus_tag="AC158Q_11030"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3340350.1"
                     /translation="MTLARETKSMGRLEGKVAFLTGAGAGIAKATAKAYVKEGAKVAI
                     IELNREAGTAAEREIRDAGGEALFVETDVTSDDSMRRAVEATAAKFGRLDVIFNCAGG
                     SLEEDLPVHQMSLDVWHRTVAVNLLHPFLSCRHGIPHLIRAGGGTIINVTSHLGLMGS
                     VKPAYAATKGGIISFTKTLAAQYVQYNIRANAIAPASIRTERQIKRYENKDYMLAEKP
                     GPAALARMAAQKLYPYSKGEPGDIAAIAVFLASDESRMLTGTTIAADGGRSSYLRYYA
                     GEGES"
     gene            complement(1985..2542)
                     /locus_tag="AC158Q_11035"
     CDS             complement(1985..2542)
                     /locus_tag="AC158Q_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HIR3340351.1"
                     /translation="MALKATICKAALQIADMDRYYYAEHALTVARHPSETGERMMVRL
                     LAFALNAHEHLAFGKGLSDTDEPDLWQKDLTGAIAHWIEVGQPDEKRLLRACGRSDRV
                     SVYAYGNRAELWWKPVADTLARAKNLAVWRIPAAASQALEKLAARTMQLQCTVQEGQL
                     WFSSAAETVHVELAALKAAGIRNSV"
     gene            complement(2552..>3275)
                     /gene="trhA"
                     /locus_tag="AC158Q_11040"
     CDS             complement(2552..>3275)
                     /gene="trhA"
                     /locus_tag="AC158Q_11040"
                     /inference="COORDINATES: protein motif:HMM:NF011669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PAQR family membrane homeostasis protein TrhA"
                     /protein_id="HIR3340352.1"
                     /translation="VIAIPGFSEPVSALTHLLGSGVFAIGGFLLVRKGRRAEGPGWNW
                     RMTGLAMFAFAAVAQLGISGAYHVLQPGLAPRLVMRQIDHTAIYFLIAATFVPVHAIL
                     FEGWRRWLILLILFSLAAIGITLSFLYFSELPEILGLTFYIAMGWVGAYSGYALYRRY
                     GWGFIRLLVWGGIAYTLGGVVEFLRQPVLLEGIVGPHELFHLAVLAGLGLHFRFIFSF
                     AGGKPESPRVSPITHFGPGRIG"
BASE COUNT          555 a         1137 c         1040 g          543 t
ORIGIN      
        1 cccggccggt attgtaaacg gcccgcgcac tataaccgcc ggatcattgc gcataaccgg
       61 cagtgcgctc gggcgccgcg ccgctgttgg cgctacaatc tcccccgttc gcggcaatgt
      121 gcagggagga acggtcatgg cagcaacctc gttggcaaat cgcggcgcgc agcgcgcccg
      181 gttcgagatc gtgccgacgc cggccgcgct cggcgcggaa gtgcgcggct tcgacctgaa
      241 atcgctcgac gatgcgacct tcaaggagct gcaccgcgcc tggctcgacc acgtgctgct
      301 ggtgttccgc gggcagtcgc tcgccgcgga agacctcgtc aggctcgtca agcgcttcgg
      361 cacgccggtg acgtcctcgg gcctgcacaa gcgcgagctg aaggagcgcg ccggcaacga
      421 gctcttcaac ctgccgcccg aagtcacggt cgtctcgaac gtgaagcagg agggcaagtc
      481 ggtcggcatc ctcggcgacg gggaagtggt gtggcattcc gatttctcgt tcaaggagcg
      541 ccccaccgcg gcgcgcatgc tgctcgccat ggaggtcccg ccgcaggagc tgggcggctt
      601 cacctatttt ctgaactgct acgcggcgta cgacgcgctg tccgcggcca tgaaaaaacg
      661 catctccggg ttgaccatca agcaggccga catcgtcgac acggccatga aagtgcggcc
      721 cggcatgtcg ctcgacatgg attatcgttc cgtgcccggc cccagccacc cggtcatctc
      781 gacgcacccg gaaaccgggc acaacatgct gttcctcggc cggcggcacg ggtcctacgt
      841 caacggcttt tcgctcgagg attccgaggc gctgctgaac gagctgtggg cccactgcac
      901 gcagccgcgg ttctggtacg agcaccggtg gtccgtgggc gacgtcgtcg tctgggacaa
      961 tcgcgccacg ctgcaccgcc gtgacccgtt cgagtccgcc agccggcgcg tgctctacgc
     1021 cgcgcaggtc gaagggcaca agccgtacga ggcgcccgac gccctcgccc gcgcgcccca
     1081 tccgcgcgcg gcgcaggcct agctctcgcc ttccccggcg tagtagcgca ggtaggagga
     1141 gcgcccgccg tcggcggcga tggtcgttcc cgtcagcatg cgcgattcgt ccgacgcgag
     1201 aaataccgcg atcgcggcga tgtcgccggg ctcgcccttc gagtagggat agagcttttg
     1261 cgcggccatg cgcgcgagcg cagccgggcc cggcttctcg gcgagcatgt agtccttgtt
     1321 ctcgtagcgc ttgatctggc gctcggtgcg gatcgaggcc ggcgcgatcg cgttggcgcg
     1381 gatgttgtac tgcacgtact gcgcggcgag cgttttggtg aacgagatga tcccgccctt
     1441 ggtcgcggca taggccggct tgaccgagcc catcagcccc aggtgcgagg tgacgttgat
     1501 gatcgttccg cctccggccc tgatcaggtg cgggatgccg tggcggcagg aaagaaacgg
     1561 gtgcagcagg ttgaccgcga cggtgcggtg ccagacgtcg aggctcatct ggtgcaccgg
     1621 cagatcctct tccagcgagc cgcccgcgca gttgaagatc acgtcgagcc ggccgaattt
     1681 cgcggccgtc gcctccaccg cgcgccgcat gctgtcgtcg ctggtcacgt cggtctccac
     1741 gaacagcgcc tcgcctcccg cgtcgcggat ctcgcgctcg gccgcggtcc cggcctcgcg
     1801 gttcaactcg atgatcgcga ccttcgcgcc ctccttcacg tacgccttcg cggtcgcctt
     1861 cgcgattccg gctcccgcgc ccgtcaggaa cgcgaccttg ccctccagcc tgcccatgct
     1921 cttcgtctcc cttgccagcg tcattcagaa ccggactcaa aatttgccac ggaaacacac
     1981 gcaatcacac agaattcctg attcccgccg ccttcaacgc ggccagttcc acatgcaccg
     2041 tttccgccgc gctgctgaac cacaactgcc cttcctggac cgtgcattgt agctgcatgg
     2101 tgcgcgccgc gagtttttcc agcgcctggc tggccgccgc cggtatgcgc cataccgcca
     2161 ggtttttcgc gcgcgcgagc gtgtccgcca ccggcttcca ccacagctcc gcccggttgc
     2221 cgtacgcgta cacggagacg cggtcggagc ggccgcaggc gcgcagcagg cgcttctcgt
     2281 ccggctggcc gacctcgatc cagtgcgcga tcgcgccggt caggtccttc tgccagaggt
     2341 cgggctcgtc cgtgtcggac agccccttgc cgaatgccag gtgctcgtgg gcattcagcg
     2401 cgaacgcgag cagccgcacc atcatgcgct cgccggtttc ggaggggtgg cgcgccaccg
     2461 tcagcgcgtg ctcggcgtag tagtagcggt ccatgtccgc gatttgcagc gcggccttgc
     2521 agatcgtcgc cttgagcgcc atggctgcac tttacccgat gcgacccggc ccgaagtgcg
     2581 tgatcggtga aacgcgggga gattcaggtt tcccgcccgc aaaggaaaat atgaaacgga
     2641 agtgaaggcc cagcccggcc agcacggcca ggtgaaaaag ctcgtgcggc ccgacgatcc
     2701 cctcgagcag gaccggctgc cgcagaaact ccactacccc gcccagcgta taggcgatcc
     2761 ctccccaaac cagcagacgg ataaaacccc atccgtaacg gcggtacagc gcgtagcccg
     2821 aatacgctcc cacccatccc atggcaatgt agaacgtcaa cccaagtatt tcgggcaatt
     2881 cactgaagta caggaagctg agcgtgatgc cgatggcggc cagtgaaaac aggatcagca
     2941 atatcagcca gcgccgccac ccctcgaaca ggatggcgtg caccgggacg aaagtggccg
     3001 cgatgagaaa gtagatggcc gtgtggtcta tctgccgcat taccagccgg ggggcaaggc
     3061 cgggctgaag cacgtggtag gcaccgctga tcccgagttg ggccacggcc gcgaaggcga
     3121 acatagccag gccggtcatg cgccagttcc acccgggacc ctccgcccgg cggcctttgc
     3181 gcaccagcag gaaccccccg atggcgaaca cccccgaacc caacaggtgg gtcagggcgc
     3241 tcacgggctc gctgaaccca gggatggcaa tcacc
//
LOCUS       DBNGSG010000423         6442 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_817142, whole genome shotgun sequence.
ACCESSION   DBNGSG010000423 DBNGSG010000000
VERSION     DBNGSG010000423.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6442)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6442)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6442
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_817142"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="AC158Q_11045"
     CDS             complement(<1..704)
                     /locus_tag="AC158Q_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohexomutase domain-containing protein"
                     /protein_id="HIR3340353.1"
                     /translation="MMKRAPIAPGIFKAYDVRGIVGKTLTPDAVEAIGQAIGTESRER
                     GCPTVAIGRDGRLSGPELSAALAAGLQKSGADVVDVGQVATPMVYFAAYHLKTHSGVM
                     VTGSHNPPDYNGLKMVVAGETLAGEAIQDLRRRIDSSRLTSGAGSYRTQSIREDYLRR
                     IVADVKLARPMKLAVDCGNGVAGATAPELYRRLGCEVAELYCEVDGNFPNHHPDPSQP
                     ANLADLQRALAEGKGEL"
     gene            complement(790..1803)
                     /locus_tag="AC158Q_11050"
     CDS             complement(790..1803)
                     /locus_tag="AC158Q_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HIR3340354.1"
                     /translation="MTRHPSLVTPPTAPYRAPLWLPGGHAQTLYAALLMPRARVAYRR
                     ERWDTPDGDFIDLDWVETLSRRLAPDTRPLVVLFHGLEGSSRSHYATAIMAAVRDAGG
                     HGVAVHFRGCSGEPNRLPRAYHSGDAREIDWVLRRLRAQNADAPLFAVGVSLGGNALL
                     KWLGENGAGVTRVVNAAVAVSAPLDLMAAGDALGRGFSLVYARNFLATLRKKSLAKLA
                     RFPGLYDGEAVRAARTLREFDDLVTAPLHGFRNTDDYWTRASSKPGLRNVRVPTLVIN
                     ARNDPFLPAPALPGAADVSAVVTLEQPEEGGHVGFVGGAFPGHFDWLPQRALEFFRPG
                     GGK"
     gene            complement(1924..2700)
                     /locus_tag="AC158Q_11055"
     CDS             complement(1924..2700)
                     /locus_tag="AC158Q_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR3340355.1"
                     /translation="MKTSAIRVRKSVREQVSKEEWETRVNLAACYRLMAEFGMVEMVA
                     NHISARVPGTHNEFLINPYGMLYEEMTASSMIRIDLSGKVLFNETDYDVNQAGYVIHS
                     AVHAARHDVDCIIHTHTLAGMAVSAMKCGILPIAQTSMRFSDIGYHDYEGVALRLEER
                     ERLVQNLGNREAMVLRNHGLLTVAPSIPECFNNMYRLERACQLQVTALSCNTELQMPP
                     AEVVQYTNHQMTSGVRRRYGELEWPALLRKLDKVDTSYKN"
     gene            complement(2857..4365)
                     /gene="tcuA"
                     /locus_tag="AC158Q_11060"
     CDS             complement(2857..4365)
                     /gene="tcuA"
                     /locus_tag="AC158Q_11060"
                     /inference="COORDINATES: protein motif:HMM:NF006130.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HIR3340356.1"
                     /translation="MSNTDFGKPDVIVVGAGNAACCAALAAREAGASVIILEAAPLDD
                     CGGNSRYTGGLMRVVYNGVDDLAQIIPDLTEEEKKTHDYGSYTAEHYYDDMGRITQYR
                     TDPDLCEILISRSFETTVWMRKKGVKFQASYGRQSYKVDDGKRYKFWGGLAAETWGGG
                     AGLIENEHKACAREGITIFYETPATGLITSDDGVVLGVRAKHKGRKVEIRSKTVVLAC
                     GGFESDAEMRARYLGPGWDLAKVRGTRYNTGLGIKMALEIGAMPYGHWSGCHAVGWDL
                     NAPPFGDVNVGDQFQKHSYPWGIMINANGERFVDEGADFRNYTYAKYGRVVLEQPGML
                     AWQVFDSKIIPMLRDEYRIKQVTKVRADTLEDLVKKMEGVNAEAALREIRDYNKAVRK
                     DLPFNMAVKDGRCTTGLKINKSNWANTIEDPPFEAYGVTCGVTFSFGGLKISNDCEVQ
                     DTGGNAIPGLYAAGELVGGIFYHNYPGGTGLTSGSVFGRISGTSAAAYAKKH"
     gene            complement(4415..4858)
                     /locus_tag="AC158Q_11065"
     CDS             complement(4415..4858)
                     /locus_tag="AC158Q_11065"
                     /inference="COORDINATES: protein motif:HMM:NF024865.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybH family protein"
                     /protein_id="HIR3340357.1"
                     /translation="MKKALFASPQDAESAFYEALTKSDLDAMMAVWADDDDIYCVHPN
                     GARLSGVEHVRESWRQIFASGQTLRFQLREQQHVQGMMLSVHSVYEYITVTGEPRPRG
                     PVIATNVFLRTERGWRMVAHHASPAPATASAEPEPRRAASPKTLH"
     gene            complement(4884..5897)
                     /gene="waaF"
                     /locus_tag="AC158Q_11070"
     CDS             complement(4884..5897)
                     /gene="waaF"
                     /locus_tag="AC158Q_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HIR3340358.1"
                     /translation="MQRILVVGPSWIGDMVLAQPLMKLLHARHAGLAIDVLAPQWTFP
                     LLERVPEVRRAIASPFAHGDLKLGERRRLGRSLAPEGYDQAIVLPNTFKSALTPFFAG
                     IPIRTGYIGELRHWLLNDARRLDKERLPQIAQRYAALGLPRGEELKPPLPALGLRVDE
                     AARRAVLDRLGLDRGRPAVALCPGAEFGPAKRWPARYFAELAQGLAARGCAVWLIGSQ
                     GDRPAGAEIERSAGGVCRNLCGDTTLTEAIDLLASSALVVSNDSGLMHLAAALGRPLI
                     ALYGSSSPAFTPPLSSSAQILKLDLPCSPCFQRECPLGHFNCMMQLTPDRVLAAVDFD
                     RIS"
     gene            complement(6025..6228)
                     /locus_tag="AC158Q_11075"
     CDS             complement(6025..6228)
                     /locus_tag="AC158Q_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HIR3340359.1"
                     /translation="MTEKTENKQRHIEVTAADLPLHCPMPSMILWNAHPRVFLPIDKT
                     GESLCPYCGTKYTLKGGAKASGH"
BASE COUNT         1012 a         2071 c         2177 g         1182 t
ORIGIN      
        1 agctcgccct tgccctcggc gagcgcgcgc tggaggtcgg ccaggttggc cggctgagac
       61 gggtccggat ggtgattggg gaaattaccg tccacttcgc aatagagttc tgcgacctcg
      121 cagccgagcc gccggtacag ttcgggcgca gtggcgcccg ccacgccgtt gccgcaatcc
      181 accgccagtt tcatcgggcg cgcgagcttc acgtcggcaa cgatacgccg gaggtaatcc
      241 tctcgaatcg actgcgtgcg gtagctgcca gctccgctcg tcagcctgct gctatcgatg
      301 cggcggcgca ggtcctggat cgcctcgccc gccagcgttt ctcccgccac caccatcttg
      361 agcccgttgt agtcgggcgg attgtgcgag ccggtcacca tcacgccgga gtgggttttg
      421 agatgatagg cggcgaagta gaccatcggc gtggcgacct ggccgacgtc gaccacgtcc
      481 gcgccgcttt tctgcaggcc ggcggcgagc gcggcggaca gttcggggcc cgacagtcgt
      541 ccatcgcggc cgatggcgac ggtggggcag ccgcgctcgc gcgattccgt cccgatcgcc
      601 tggccgatgg cttcgactgc atcgggggtg agcgttttgc cgacgatgcc gcggacgtcg
      661 taggccttga atatcccggg agctatgggt gcgcgtttca tcatggtcgg cgattatctc
      721 atgcgggtga acgggaaccg gcaaacaggg aataggacaa tcccatgagc agaatgtttc
      781 ttccattttc tacttgccgc ctcccgggcg gaagaattcc agcgcgcgtt gcggcagcca
      841 gtcgaagtgg ccgggaaacg cgccgccgac gaaacccacg tggccgcctt cctccggctg
      901 ctcgagtgtc accacggcgg atacgtcggc tgcgccggga agcgccgggg ccgggagaaa
      961 cgggtcattc ctggcattga tcacgagcgt cgggacgcgg acatttctca atcccggctt
     1021 gctgctcgcg cgcgtccagt agtcgtcggt gttcctgaag ccgtgcagcg gcgcggtgac
     1081 caggtcgtcg aactcgcgca gggtgcgcgc tgcccgcacg gcctctccgt cgtacaggcc
     1141 gggaaagcgc gccagcttgg cgaggctttt cttccgcagg gtggcgagga agttgcgcgc
     1201 gtagacgagg ctgaagcccc gcccgagcgc atctcccgcc gccatcaggt cgagcggcgc
     1261 ggagaccgca acagcggcgt tgacgacgcg ggttacgcca gccccgtttt cgccgagcca
     1321 tttgagcagc gcgttgccgc ccagcgagac gccgaccgcg aagagcgggg cgtcggcatt
     1381 ctgcgcccgc agccgccgca ggacccagtc gatttcccgg gcatcgcctg aatgataggc
     1441 gcgcgggaga cgattgggct cgccgctgca tccccggaaa tggaccgcga cgccgtgccc
     1501 gcccgcatcc cgcaccgctg ccatgatcgc ggtcgcgtaa tgactccgtg acgagccctc
     1561 cagcccgtga aacagcacca ccagggggcg agtgtcgggt gcaaggcgcc gactgagggt
     1621 ttccacccaa tccaggtcga tgaagtcgcc gtcgggcgtg tcccagcgtt cacggcggta
     1681 agcgacccgt gcccgcggca tcagcagcgc tgcataaagg gtctgcgcgt ggccgccggg
     1741 cagccataac ggcgcccgat agggcgccgt agggggcgtg acgagtgacg ggtgacgagt
     1801 gatgggagac tcccgcgcga gcgcgggtac cttatcacct atcacttatc acccatcacc
     1861 aacgccgcag gcgacaaaaa agcggggccg caaggcaccc gcttttttac gccatggcgg
     1921 cgcttagttc ttgtaggacg tgtcgacctt gtcgagcttg cgcagcagcg ccggccattc
     1981 gagttcgccg tagcggcggc gcacgccgga ggtcatctgg tggttggtgt actggaccac
     2041 ttccgcgggc ggcatctgca gctccgtgtt gcaggagagc gcggtcacct ggagctggca
     2101 ggcgcgctcc agccggtaca tattgttgaa gcactccggg atgctcggcg ccacggtcag
     2161 aaggccgtgg ttgcgcagca ccatcgcctc gcggttgccg aggttctgca ccagccgctc
     2221 ccgctcctcc agccgcagcg ccacgccctc gtaatcgtgg tagccgatgt ccgagaagcg
     2281 catcgaggtc tgagcgatcg gcaggatgcc gcacttcatc gcagaaaccg ccatgccggc
     2341 gagggtatgc gtgtggatga tgcagtccac gtcatggcgc gcggcgtgca cggcgctgtg
     2401 gatgacgtac cccgcctggt tgacgtcgta atccgtttcg ttgaacagca ccttcccgga
     2461 gaggtcaatg cggatcatgg aggacgcggt catctcctcg tagagcatcc cgtaggggtt
     2521 gatcaggaac tcgttgtgcg ttcccggcac gcgcgccgag atgtgattgg cgaccatctc
     2581 gaccatgccg aattccgcca tcaggcggta gcacgccgcg aggttgacgc gcgtttccca
     2641 ctcttccttg ctgacctgct cgcgcaccga cttcctgacg cggatagctg aagtcttcat
     2701 ggctgcctcc aatagggtga ctcgccgatt ctagcgggtt cgtgcccggc cgggataccg
     2761 cgtttccaca tgctgaacag atgtcagccg ccaaacgcga tgccggtggc cggcccaatt
     2821 cgccctgtgg ttctgccgac cgctgacagc cgacggctag tgtttcttgg cgtacgcggc
     2881 ggcgctggtg cccgagatcc ggccgaatac cgacccggag gtcaggcccg tgccgcccgg
     2941 gtagttgtgg tagaagatgc cgcccaccag ctcgcccgca gcatagaggc cgggtatggc
     3001 attgccgccg gtgtcctgga cctcgcagtc attgctgatc ttgagcccgc cgaagctgaa
     3061 ggtgacgccg caggtgacgc cgtaggcttc gaacggagga tcctcgatgg tgttggccca
     3121 gttcgacttg ttgattttca gtccggtggt gcagcggccg tccttgaccg ccatgttgaa
     3181 cgggaggtcc ttgcgcaccg ccttgttgta atcgcggatt tcgcgcaagg cggcctcggc
     3241 gttcacgccc tccattttct tcaccaggtc ctcgagcgtg tcggcgcgga ccttggtgac
     3301 ctgcttgatg cggtactcgt cgcgcagcat gggaatgatc ttggagtcga aaacctgcca
     3361 cgccagcatg ccgggttgct cgagcaccac gcgcccgtac ttggcgtagg tgtagttgcg
     3421 gaagtccgcg ccctcgtcaa cgaagcgctc gccgttcgcg ttgatcatga tgccccaggg
     3481 gtagctgtgc ttctggaact ggtcgccgac gttgacgtcg ccgaagggcg gcgcattgag
     3541 gtcccagccc accgcgtggc agcccgacca gtggccgtag ggcatggcgc cgatttcgag
     3601 cgccatcttg atgccgaggc cggtgttgta gcgggtgccg cgcaccttgg cgaggtccca
     3661 gcccgggccg aggtagcgcg cgcgcatctc cgcgtcggac tcgaagccgc cacaggcgag
     3721 caccaccgtc ttcgagcgga tctcgacttt cctgcccttg tgcttcgcgc gcacgccgag
     3781 caccacgccg tcgtcgctgg tgatcagccc ggtagccggt gtttcataga agatcgtgat
     3841 gccctcgcgc gcgcaggcct tgtgctcgtt ctcgatcagg ccggcgccgc cgccccaggt
     3901 ctcggccgca agtccgcccc agaacttgta gcgcttgccg tcatcgacct tgtacgactg
     3961 gcgcccgtag ctcgcctgga acttgacgcc tttcttgcgc atccacaccg tcgtctcgaa
     4021 gctgcgcgag atcaggattt cgcagagatc ggggtcagtc cggtactggg tgatgcggcc
     4081 catgtcgtcg tagtagtgct cggcggtgta ggagccgtag tcgtgggtct tcttttcttc
     4141 ctcggtgagg tcggggatga tctgcgccag atcgtcaacg ccgttgtaga ccacgcgcat
     4201 gagcccgccg gtgtagcggc tgttgccgcc gcagtcatcc agcggcgcag cctccaggat
     4261 gatgacggac gcgccggctt cgcgagcggc gagggcggcg cagcaggctg cattgccggc
     4321 gccgaccacg atgacgtcag gttttccgaa atcggtattg ctcattgctt tcctttaagt
     4381 taccagcggt ttttcgaatc acgaatcacg gctttcagtg cagggtcttc ggcgacgccg
     4441 cgcggcgcgg ttcgggctcg gcgctcgccg tggcgggggc cggcgatgcg tgatgcgcga
     4501 ccatgcgcca gccgcgctcg gtcctcagaa agacgtttgt cgcgatgacc gggccgcgcg
     4561 ggcgcggctc gcccgtcacc gtgatgtatt cgtagacgct gtgcactgac agcatcatgc
     4621 cctggacgtg ctgctgctcg cgcagctgga agcgcagcgt ctgcccgctg gcgaatatct
     4681 gccgccacga ttcacgcacg tgctcgacgc ccgagaggcg cgcgccgttg ggatgcacgc
     4741 agtagatgtc gtcgtcgtcg gcccacaccg ccatcatcgc gtcgagatcg gacttggtaa
     4801 gcgcctcgta gaaagccgac tcggcatcct gggggctggc gaacagtgct ttcttcatcg
     4861 gtgcgggcac gggacagggc tgctcatgaa attctatcaa aatcgacggc cgccaagacc
     4921 cggtcgggcg tcaactgcat catgcagttg aagtgcccga gcgggcactc gcgctgaaag
     4981 caggggctgc agggcaggtc gagcttgagt atctgcgcgc tcgacgaaag cggcggggtg
     5041 aacgccgggc tgctcgagcc gtacagcgcg atgagcggtc ggccgagcgc ggccgccagg
     5101 tgcatgagcc ccgagtcgtt gctgacgacc agcgcgctcg acgccagcag gtcgatcgct
     5161 tcggtcagcg tcgtgtcgcc gcagaggttg cggcagacgc cgccggcgga ccgctcgatc
     5221 tcggctcccg ccggccggtc gccctgcgag ccgatcagcc agaccgcgca gccccgcgcg
     5281 gcgagcccct gcgcaagctc cgcgaaatag cgtgccggcc agcgcttggc cggcccgaat
     5341 tcggcgcccg ggcagagcgc aaccgcgggg cggccccgat cgagtccgag ccggtcgagc
     5401 acggcgcgcc gcgccgcctc gtccacgcgc aggccgagcg cgggcagggg cggtttcagt
     5461 tcctcgccgc gtggcaatcc cagcgccgcg tagcgctgtg cgatctgcgg caggcgctcc
     5521 ttgtccaggc ggcgcgcgtc gttcagaagc cagtggcgca gttcgccgat gtagccggtg
     5581 cggatcggga tcccggcgaa aaaaggcgtc aacgccgact tgaaggtgtt gggcagcacg
     5641 atggcctggt cgtagccttc cggggcgagg ctgcgcccca ggcggcggcg ctcgccgagt
     5701 ttcaagtcgc cgtgggcaaa cgggctggct atcgcccgcc ggacttcggg aacgcgttcc
     5761 agcaggggga aagtccactg tggagcgagc acatcaatcg cgaggccggc gtggcgcgcg
     5821 tgcagaagct tcatgagcgg ctgcgccagc accatgtcgc cgatccacga aggacccaca
     5881 accaggatgc gctgcatggt tgtgggtcct tatgagggat gagggatgag ggatgaggga
     5941 tgagggaaag gcctcattcc aatctcctgt ttttcattcc tccgacctcc gccctccgcc
     6001 ctcatccctc ggcggtggga cgcgttagtg tcccgacgcc ttggcgccgc ccttgagcgt
     6061 gtacttcgtg ccgcaatacg gacacagcga ctcgccggtc ttgtcgatcg gcaggaaaac
     6121 gcgcgggtgg gcgttccaca ggatcatgga cggcatcggg caatgcagcg gcaggtccgc
     6181 cgcggtgacc tcgatgtggc gctgcttgtt ttccgttttc tctgtcatgg ggagtctgat
     6241 tcgagtgacg agtgtcgggt gtcgagtgcc gggtgtcgag tgccgggtgt cgagtggggg
     6301 cagcccttta tgttccgctc cctcgacact ccatcctcga cactccctga ctggtcaggc
     6361 ggcgaccggc gtgagccagt cgcggtactg gtcggacttg cccgagacgc agtcgaagaa
     6421 cgtcttttgc agcttttccg tg
//
LOCUS       DBNGSG010000424         5196 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3194919, whole genome shotgun sequence.
ACCESSION   DBNGSG010000424 DBNGSG010000000
VERSION     DBNGSG010000424.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5196)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5196)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5196
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3194919"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..78
                     /locus_tag="AC158Q_11080"
     CDS             <1..78
                     /locus_tag="AC158Q_11080"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase"
                     /protein_id="HIR3340360.1"
                     /translation="VFDDGPQPTGLRYCINSASLKFERG"
     gene            199..753
                     /locus_tag="AC158Q_11085"
     CDS             199..753
                     /locus_tag="AC158Q_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HIR3340361.1"
                     /translation="MKFLFDIFPVVLFFVAFKFYDIYVATAVAIAATFVQIGWTWLRH
                     RKVENMLWVSLAVIVLFGGATLLLQNETFIKWKPTVLYWLFAGALAVAQLGFKKNLIR
                     AMMGAQIELPDAVWLRLLASWIAFFAVMGVLNLLVAFNFTTDTWVNFKLFGGIGLMLV
                     FVVLQALMLSRHIEDKKADQGPPT"
     gene            750..1013
                     /locus_tag="AC158Q_11090"
     CDS             750..1013
                     /locus_tag="AC158Q_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HIR3340362.1"
                     /translation="MNVARQIQEKLAVLAPESLEILDESGQHAGHEGARGGGGHFQLV
                     IVSTQFAGKPAQLRHRMVYDALGPLMKKEIHALAIKAYAPGEI"
     gene            1037..1831
                     /locus_tag="AC158Q_11095"
     CDS             1037..1831
                     /locus_tag="AC158Q_11095"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR3340363.1"
                     /translation="MTSTLYALLLAGLLAAGTAHAQNTPVKVNGVTISQQRMDFFVKN
                     LVAQGRPDTPELRSSVREHLINQELLAQEAKRRGLDKNTEVAMQLEIARQSVLSDSLM
                     RDVMRGNLTVNDDAVRKEYERLKAQLGTREFRARHILVDKEDEAKEIIAQIKKGASFE
                     KLAAERSKDSGSKVRGGELDWSPAGQYVKPFADALNRLKKGQMTDSPVQTNFGWHVIR
                     VEDERPAKVPAYDQVKGNLQQQMQLQTQKQAFEKTITELRAKAKIE"
     gene            complement(1956..>5196)
                     /gene="purL"
                     /locus_tag="AC158Q_11100"
                     /gene_synonym="purI"
     CDS             complement(1956..>5196)
                     /gene="purL"
                     /locus_tag="AC158Q_11100"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HIR3340364.1"
                     /translation="EHCRHKIFNSSWILDGKPQEHSLFGMIRTTHQKNPRGTVVAYTD
                     NAAIMEGASIARLYPQNGGEYRYTDALTHSVLKVETHNHPTAISPFPGAATGAGGEIR
                     DEGATGRGAKPKAGLTGFTVSNLHIPGFAQPWERDGYGRPRRIASALQIMLEGPIGGA
                     SFNNEFGRPNLAGYFRTFEQRVAGEMRGYHKPIMIAGGIGNLSAEHAFKRGLASGTVL
                     IQLGGPGMLIGLGGGAASSMDTGSNQEDLDFDSVQRGNADIQRRAQEVIDRCWALGEE
                     NPILSIHDVGAGGLSNALPELVHSAKRGGRFDLRSIPSEEPGMSPLQIWCNEAQERYV
                     LAIAPRDLERFRALCERERCPFTVVGEATDDGRLVVSDAVFGNNPVDMELAVLLGKPP
                     KMTRDVTHVRHELPPLDTAGLDIREAAYRVLRLPSVADKTFLITIGDRTVGGLTARDQ
                     MVGPWQVPAADAAVTLMGFNTCRGEAFAMGERTQLALVSPEASGRMAVGEAITNIAAA
                     PIADIGDIKLSANWMAAAGHPGEDAALFDTVRAVALELCPQLGISIPVGKDSLSMKTA
                     WEENGARKEVTAPLSLIITAFAPVPDVRRALTPQLRSDAGDTDLLLVDLGAALCRMGG
                     SALAQVYARLGNDAPDADATRLRSFFSAVQRLGREGKILAYHDRSDGGLFATVCEMAF
                     AGRVGVALNLDAAVTGGKSVIEFLFNEELGAVLQVRRADLPVVREVFSESGLGDQLHL
                     IGGLSQDDTLRLAAGVKELFREKLGDLHRAWSETTWHMQRLRDNPACAQQEHDRLLDR
                     GDPGLHASLAFRMDEDVAAPYVNRGARPRIAILREQGVNGHLEMAAAFDRAGFAAVDV
                     HMSDIIGGRISLADFKGFAACGGFSYGDVLGAGEGWAKSVLFNSRARAAFEAFFRRAD
                     SFALGVCNGCQMMSNLHELIPGAGAWPHFVRNQSEQFEARFVMVEVQRSPSLFFEGMA
                     GSRIPIVVSHGEGYAEFGGAAALKAAEPLVALRFTDNRGEVSEVYPYNPNGSPGGITG
                     LTTADGRFTILMPHPERVFRAVQNSWCPEGWGEDGPWMRIFRNARRWCA"
BASE COUNT          951 a         1692 c         1624 g          929 t
ORIGIN      
        1 gtcttcgatg acggccccca gcccaccggg ctgcgctact gcatcaactc ggcgtcgctc
       61 aaatttgaga gagggtgaac gattagcggt gagccggcac gtctgctccg cgtcgcaagc
      121 gcgccccctg tcgtaagtta ttgactgaca accgcttacc gcttactgct cacagcttac
      181 cgggtataat acgcgcccat gaaattcctg ttcgatattt tcccggtcgt cctgttcttc
      241 gtcgccttca agttctacga catttacgtc gccactgccg tcgccatcgc ggcgactttc
      301 gtgcagatcg ggtggacctg gctgcggcac cggaaggtcg agaacatgct ttgggtgagc
      361 ctcgcggtga tcgtgttatt cggcggcgcc acgctcctgc tgcagaacga gactttcatc
      421 aaatggaagc cgaccgtgct ctactggctg ttcgccggcg cgctggccgt agcgcagctg
      481 ggatttaaaa agaacctgat ccgcgccatg atgggagcgc agatagagct ccccgatgcc
      541 gtctggctca ggctgctcgc cagctggatc gccttcttcg ccgtgatggg cgtgttgaat
      601 ctccttgtgg cctttaactt cacgaccgac acctgggtca atttcaagct gttcggcggc
      661 atcggcctca tgctggtctt cgtggtgctg caggcgctga tgctgtcgcg ccacatcgaa
      721 gacaagaaag ccgatcaagg gccgccgaca tgaatgtggc ccggcaaatc caggagaaac
      781 tcgctgttct ggcgcccgaa tcactcgaga ttctcgatga atcgggtcaa cacgccggcc
      841 acgaaggcgc gcgcggcggc ggcggccact ttcagctggt catcgtgtcc actcaattcg
      901 cgggcaagcc ggcccagttg cgccaccgca tggtgtacga tgcgctgggc ccattgatga
      961 aaaaagaaat ccacgccctg gccatcaagg cctacgcgcc gggcgaaatt tgatatcccc
     1021 ctctaaagga acgtagatga cctcaactct ttatgcattg ttgctggccg ggctgcttgc
     1081 cgcgggcacc gcgcatgcgc aaaacactcc ggtcaaggtc aacggcgtga ccatctcgca
     1141 gcagcgcatg gacttcttcg tgaagaatct cgtcgcccag ggccggcccg acacccctga
     1201 gcttcggagc agcgtccggg aacacctgat caaccaggag ctgctggcgc aggaagcgaa
     1261 gcgacgcggc ctggacaaga acaccgaagt tgcgatgcag cttgagatcg cccgccagag
     1321 tgtgctgtcc gattctctga tgcgcgatgt catgcgcggt aacctgaccg tgaacgacga
     1381 cgctgtcagg aaagaatacg agcggctgaa ggcccagctc ggcacgcggg aattccgcgc
     1441 ccgccatatc ctggtggaca aggaggacga ggccaaggag atcatcgccc agatcaagaa
     1501 gggcgcgagc ttcgagaagc tcgcagcgga gcggtccaag gattccggca gcaaggtgcg
     1561 cggaggcgaa ctggattgga gcccggcggg acagtacgtc aagccttttg ccgatgccct
     1621 gaaccgtctc aagaagggcc agatgaccga ctcgccggtg cagaccaatt tcggctggca
     1681 cgtgatccgg gtggaagacg agcggccggc caaggtcccg gcgtacgatc aggtcaaggg
     1741 caacctgcag caacagatgc aactgcagac tcagaagcag gctttcgaaa aaacgattac
     1801 cgaactgcgc gccaaggcca agatcgagtg acaggtgacg agtgacaggt gatggaggtt
     1861 gagtaaactg ctcacctcat aaaccaaata aaacggcgca ccgtggtgcg ccgtttttgt
     1921 tgaaggcgac gttgaggctt atcacttatc acaccctatg cacaccaacg gcgggcattg
     1981 cggaatattc tcatccacgg cccgtcctcg ccccagccct cggggcacca ggaattctgc
     2041 acggcccgga agacgcgctc ggggtgcggc atgaggatgg tgaatcggcc gtcggccgtg
     2101 gtgaggccgg tgatcccacc gggcgacccg ttcgggttgt aaggatagac ctcgcttacc
     2161 tcgccccggt tgtccgtgaa gcgcagcgcg accagcggtt ctgccgcttt cagcgcggcg
     2221 gcgccgccga actcggcata gccctcgcca tgcgaaacca cgatcgggat gcggctcccg
     2281 gccatgccct cgaagaacaa cgaaggcgag cgctgcactt ccaccatcac gaagcgcgcc
     2341 tcgaactgtt ccgactggtt gcgcacgaaa tgcggccatg cgcctgcgcc ggggatcagc
     2401 tcgtgcaggt tgctcatcat ctggcagcca ttgcatacgc ccagcgcgaa tgaatcggcg
     2461 cgcctgaaga aggcttcgaa cgccgcgcgc gcccgggaat tgaacagtac ggacttcgcc
     2521 cagccctcgc ccgctcccag gacgtcacca taggagaacc cgccgcaggc ggcaaaccct
     2581 ttgaaatccg cgaggctgat gcgccccccg atgatgtcgc tcatgtgcac gtcaaccgcc
     2641 gcgaagcccg cccggtcgaa ggcggccgcc atttcgaggt gaccgttgac gccctgctca
     2701 cgcaggatgg cgatgcgcgg gcgggcgccc ctgttcacgt aaggggccgc aacgtcttcg
     2761 tccatcctga acgcgagact ggcatggagt cccgggtcgc cccgatcgag caggcggtcg
     2821 tgctcctgtt gtgcgcacgc cgggttgtcg cgcagccgct gcatgtgcca ggtggtctcc
     2881 gaccaggcgc ggtgcaggtc gccgagcttt tcacggaaca gttccttgac tcctgcggcc
     2941 agcctgagcg tgtcgtcctg actcagcccg ccgatcaggt gaagctggtc ccccaggccg
     3001 gattccgaga agacttcgcg cacaacgggc aggtcggcac ggcgcacctg gagcacggcg
     3061 ccgagttcct cgttgaacag gaactcgatg acggacttgc cgccggttac ggcagcgtcg
     3121 aggttgagcg cgaccccgac ccggcccgcg aaagccatct cgcacaccgt cgcgaacagg
     3181 ccgccatccg agcggtcgtg ataagcgagg attttccctt cgcggccgag gcgctgcacg
     3241 gcactgaaga atgacctgag ccgcgtcgcg tccgcgtccg gcgcgtcatt cccgagccgg
     3301 gcgtagacct gggcgagggc cgagccgccc atccggcaca gcgccgcccc gagatccacg
     3361 agcagcaggt ccgtgtcgcc ggcatcggat cgcaactgcg gtgtaagcgc acgacgtacg
     3421 tccggcacag gcgcgaatgc cgtgatgatc agggacaaag gcgccgtcac ttccttccgc
     3481 gcgccgttct cttcccatgc cgtcttcatc gaaaggctgt ccttgccgac cggtatgctg
     3541 atcccgagct ggggacagag ttcgagcgcg accgcccgca ccgtgtcgaa cagcgcggca
     3601 tcctcccccg gatgacccgc cgccgccatc cagttggccg acagcttgat gtcgccgata
     3661 tcggctatcg gcgccgcggc gatattcgtg atcgcctccc ccaccgccat gcgcccggag
     3721 gcttcagggc tcaccagggc gagttgcgtg cgctctccca tggcaaatgc ctcgccgcgg
     3781 caggtattga atcccatcag cgtcaccgcg gcatcggctg ccggcacctg ccagggaccg
     3841 accatctggt cgcgtgcggt cagaccgccc acggtacggt cgccgattgt gatgagaaag
     3901 gtcttgtcag ccacggatgg cagtcgcaaa acgcgatagg ccgcttcgcg gatgtcgagc
     3961 cccgccgtat cgagcggcgg cagttcatgc cggacatgtg tgacatcgcg cgtcattttc
     4021 ggaggcttcc ccagcagcac ggccagttcc atgtccaccg ggttgttgcc gaatactgcg
     4081 tcgctcacga ccagccgccc gtcgtcggtc gcttccccga ccacggtgaa cgggcagcgc
     4141 tcgcgctcgc aaagcgcccg gaagcgctcg aggtctcttg gggcgatcgc cagcacgtaa
     4201 cgctcctggg cctcgttgca ccagatctgc agtggcgaca tgccgggctc ttcactcggg
     4261 atgctgcgca gatcgaagcg cccgccacgc ttcgccgagt gcaccagttc cggcagcgca
     4321 ttggacagcc cgccggcgcc gacgtcgtgt atcgacagga tgggattttc ttcgcccagc
     4381 gcccagcaac ggtcgatcac ttcctgcgcg cggcgctgaa tatcggcgtt gccgcgctgc
     4441 accgagtcga agtccaggtc ttcctggttg gagccggtat ccatgctgga agccgctccg
     4501 ccgccgagcc cgatcagcat gccgggaccg cccaactgga tcaggaccgt gccggacgcc
     4561 agcccgcgct tgaacgcatg ctccgccgaa agattgccga tgccgccggc gatcatgatc
     4621 ggcttgtggt agccgcgcat ctcgcccgcg acgcgctgct cgaaggtgcg gaaataaccc
     4681 gccaggttgg ggcggccgaa ttcgttgttg aatgacgccc cgccgatcgg accttccagc
     4741 atgatctgca gggccgaagc gatccgccgc ggcctgccat agccgtcgcg ctcccagggc
     4801 tgcgcaaagc cggggatgtg gaggttggac accgtgaacc cggtcagccc ggccttgggt
     4861 ttggcgccgc ggccggttgc accttcgtcg cggatttccc cgcccgcccc ggtcgcggcg
     4921 ccggggaatg gtgaaatcgc ggtcgggtga ttatgcgtct ccaccttcag cacggaatgt
     4981 gtaagcgcat cggtgtaacg gtactctccg ccgttctgag ggtacaggcg ggcgatgctc
     5041 gcgccttcca tgatcgcggc gttgtcggta taggcgacca ccgtcccgcg cgggttcttc
     5101 tggtgcgtgg tgcgtatcat gccgaacaac gagtgctcct gcggctttcc atccagtatc
     5161 cagctcgagt tgaagatctt gtggcggcag tgctcg
//
LOCUS       DBNGSG010000425         2705 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3533515, whole genome shotgun sequence.
ACCESSION   DBNGSG010000425 DBNGSG010000000
VERSION     DBNGSG010000425.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3533515"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="AC158Q_11105"
     CDS             complement(<1..783)
                     /locus_tag="AC158Q_11105"
                     /inference="COORDINATES: protein motif:HMM:NF037184.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HIR3340365.1"
                     /translation="MPHISRLRGRNALSAFRAAKLSQSIIAAVPRVSGIQAEFWHFAA
                     LSRAVSKDETARLERILAYGPEAIGVESQGDLVLVVPRIGTISPWSSKATDIARHCGL
                     EAVERIERGTAYWIATRDGKPLASGDRAALVPLIHDRMTETVLGDFESAARLFEHIKP
                     VPLAAVDLLKGGVAALQRANSEMGLALSEDEIEYLYQQFTRVGRNPTDVELMMFAQAN
                     SEHCRHKIFNSSWILDGKPQEHSLFGMIRTTHQKNPRGTVVAY"
     gene            924..2417
                     /locus_tag="AC158Q_11110"
     CDS             924..2417
                     /locus_tag="AC158Q_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HIR3340366.1"
                     /translation="MTVHAAHGTPIYPTAGIRAIEAAAVAGKNPPKLMERAGLAAAEI
                     ARDISGGTGKPVLVLAGPGNNGGDAFVVARHLKKWFFKVTVAYAGDEKKLTADARAAL
                     AAWRKAGGTLSPAPPAARQWGLVVDGIFGIGLERDVSGRYAEWVASINGLGAPILALD
                     VPSGLHSDTGRVMGCAVRATHTATFIALKAGLLTLDGPDHCGEIHLRTLDLDAQSLSP
                     ASGFLAGREVLAGVLKPRPLNSHKGDYGSVGVIGGDHGMVGAALLAGRAALKLGAGRV
                     YLGLLAGDAPLLDAGQPELMLRSADEVLKFNHLSALAIGPGLGQMPDAAFYLGWALES
                     SLPLVLDADALNLIAFDRHLAGLLNSRQGPSLLTPHPAEAARLLGTGTREVQNDRVAA
                     ALALAKRLGCLVVLKGAGSVCAAPDGSWHINTSGNPGMASAGMGDVLTGMVAALLSQG
                     AEPKTALLAAVYLHGAAADRAVVNGTGPIGLTATETIETARGLLNQR"
     gene            complement(2460..>2705)
                     /locus_tag="AC158Q_11115"
     CDS             complement(2460..>2705)
                     /locus_tag="AC158Q_11115"
                     /inference="COORDINATES: protein motif:HMM:NF014584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HIR3340367.1"
                     /translation="YLIGIAVEMVMQLQLVSAEWRLFIITGFLGSLTTFSTFSVEAVE
                     LMAMQRYGWAMLHVGSHLAGSLVMTVLGIYTVRWLTI"
BASE COUNT          463 a          874 c          883 g          485 t
ORIGIN      
        1 ataggcgacc accgtcccgc gcgggttctt ctggtgcgtg gtgcgtatca tgccgaacaa
       61 cgagtgctcc tgcggctttc catccagtat ccagctcgag ttgaagatct tgtggcggca
      121 gtgctcggaa ttggcctgcg cgaacatcat cagctcgacg tcggtcgggt tgcggccgac
      181 gcgggtgaac tgctggtaga ggtactcgat ctcgtcctcg gacagcgcca ggcccatttc
      241 gctgttggcc cgctgcagcg ccgccacgcc tcctttcagc aggtccaccg cagccagcgg
      301 cacgggcttg atgtgctcga acaatcgggc ggcactttca aaatccccaa gcactgtttc
      361 cgtcatgcga tcgtgaatca ggggtaccaa tgccgcccga tcacctgaag caagaggctt
      421 gccgtcgcgc gtcgcgatcc agtatgccgt gccgcgctcg atgcgctcca ccgcctccag
      481 tccgcaatgg cgcgcgatgt cggtcgcttt cgaagaccag ggggaaatgg tgccgatacg
      541 cggcacgacc agcaccaggt cgccttgcga ttcaacgccg atcgcctcgg ggccgtaggc
      601 gagaatccgc tcgagacgcg ccgtttcgtc cttcgacacc gcgcgggaga gcgcggcgaa
      661 atgccagaac tcggcctgga tgccggagac gcggggaacg gcggcgatga tggactgcga
      721 gagcttggct gcgcggaagg ccgagagcgc gttgcgtccc ctgagcctgg atatgtgcgg
      781 cattaccgtg agcgttgtcg caattcgcgc gggttgaacg gcgcggcgcg tctgtctcga
      841 acccgcgcac ttaaaacggc catgctgcca gggtccgttt taagcgttat tttacccgaa
      901 atgacgttaa actacacata cttatgacgg tacacgccgc tcacggcacg ccgatttacc
      961 cgaccgccgg cattcgcgcc atcgaggcgg cggctgttgc cgggaaaaat cccccgaagc
     1021 tgatggaaag agccgggctt gcggcggccg agatcgcgcg cgacatctcc ggcggcaccg
     1081 gcaagcccgt gctggtattg gctggacccg gcaacaatgg cggcgacgcg ttcgtcgtgg
     1141 cgcggcatct gaagaaatgg tttttcaagg tcacggtggc ctacgccggc gacgagaaaa
     1201 agcttacggc tgacgccagg gccgcgttgg ccgcctggcg caaggccggc ggcaccctca
     1261 gccccgctcc gcccgccgcg cggcagtggg gtctggtggt ggatggtatc ttcggcatcg
     1321 gtctcgaacg cgatgtcagc gggcgttatg ccgagtgggt cgcctcgatc aacggtctgg
     1381 gcgccccgat actcgcactg gatgttccga gcggcctgca ctccgatacc ggtcgtgtca
     1441 tgggttgcgc ggttcgcgcg acccatacgg ccaccttcat tgcgctcaaa gccggcctgc
     1501 tcacgctcga tggccccgac cattgcggtg aaattcatct gcgcacgctc gacctcgatg
     1561 cgcagtcgct gagtcccgcc agcggttttc tggccgggcg cgaagtgctt gccggcgtgc
     1621 tgaagccgcg ccccctgaac agccacaagg gtgactacgg cagcgtcggc gtgatcggtg
     1681 gcgatcacgg catggtgggc gcagcgttgc ttgccgggcg cgccgcgctc aagctggggg
     1741 ccgggcgtgt ctatcttggg ctgctcgcgg gcgatgcgcc gctgctcgac gccggtcagc
     1801 cggagctgat gctgcgcagc gccgatgagg tgttgaagtt caatcacctc tccgccctcg
     1861 ccataggccc cggcctgggc cagatgcccg atgccgcgtt ctacctggga tgggccctgg
     1921 aatcctccct gcccctggtg ctggacgccg acgccttgaa cctgatcgca ttcgaccggc
     1981 atctcgccgg cctgttgaat agccgccagg gcccatcgct gctcactcca catcccgccg
     2041 aagccgcgcg gctgctcggc accggcacgc gcgaggttca aaacgaccgg gtagccgccg
     2101 cactcgcgct tgcgaagcgt cttggctgcc tggtggtcct gaaaggggcc ggcagcgtgt
     2161 gcgcggcgcc tgacggaagc tggcacatca atacttccgg caaccccggt atggccagcg
     2221 caggcatggg ggacgtgctg acaggcatgg tggccgccct gctgtcgcag ggtgccgagc
     2281 cgaagactgc gctgctggcc gccgtctatc tgcatggtgc ggcagctgat cgcgccgtgg
     2341 tcaacggcac aggcccgatc gggctgacgg cgactgaaac gattgaaacc gcccgggggc
     2401 tactcaacca gcgttagcgc cggaaggcgc aattcccttt cgtcttccgc ctttcgactt
     2461 cagatggtga gccagcgcac ggtgtaaatc cccagcacgg tcatcaccag cgatccggcc
     2521 aggtgtgaac ccacatgcag catcgcccag ccgtagcgct gcatggccat gagttccacc
     2581 gcttcgacag aaaaagtcga gaaagtggtc aggctgccaa ggaaaccggt gatgatgaag
     2641 aggcgccatt ccgcggagac gagttggagc tgcataacca tctccaccgc gatgccgatc
     2701 aggta
//
LOCUS       DBNGSG010000426         2221 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2854762, whole genome shotgun sequence.
ACCESSION   DBNGSG010000426 DBNGSG010000000
VERSION     DBNGSG010000426.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2854762"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2221
                     /locus_tag="AC158Q_11120"
     CDS             <1..>2221
                     /locus_tag="AC158Q_11120"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3340368.1"
                     /translation="QIFNVGAERMLGYAAADVVNKITPADISDPQELIARAKTLSAEL
                     ATPITPGFEALVFKASRGIEDIYELTYFRKDGSRFPAIVSVTALRDAQDAIIGYLLIG
                     TDNTARKQAEEALLKAGALQSAIFNSANFSSIATDAKGVIQIFNVGAERMLGYTAAEV
                     MNKITPADISDPQEVIARAKTLSLELATPIAPGFEALVFKASRGIEDIYELTYIRKDG
                     SRFPAIVSVTALRDQRDGIIGYLLIGTDNSARKEVEAAQALLDQRLRDQQFYTRSLIE
                     SNIDALMTTDPQGIISDVNQQMIALTGRTRDELIGAPCKTFFTDPARAEAAIKRVLTE
                     NKVSNYELTVLAQNGEETVVSYNAATFHNRDRKLQGVFAAARDVTELKRFERTLQEKN
                     VELEHASSMKSEFLATMSHELRTPLNAIIGFSEALKDGLIGPMSDTQQEHVGDIFTSG
                     QHLLSLINDILDLSKVEAGMMVLELEPVDLKSLLSNSLSIVREKAAAQRIQIELEAGE
                     DLGQPQLDMRKTKQIVYNLLSNAVKFSATGGRVILRARRVPRGAVGTLPGDWPVHGFP
                     LADNEYAEFLEICVRDSGIGLSRENMAKLFQAFSQIDSSLARKFEGTGLGLAMVKQLA
                     ELHGGTVAVASAEGEGARFAAWLPLRAPAEAAIGLSQSARPTPAGDAGAGAPAAVETR
                     VRNALVIDDDDRAADLLRLYLEAEGFTVLRAVSAEDALLLVPRQLLALITLDLQMYGM
                     "
BASE COUNT          437 a          754 c          687 g          343 t
ORIGIN      
        1 ccagatcttc aacgtcggcg ccgagcgcat gctgggctac gccgccgccg acgtggtgaa
       61 caagatcacc ccggcagaca tctccgatcc ccaggaactg atcgcgcgcg ccaagacgct
      121 cagcgccgaa ctcgcaaccc cgatcacgcc gggtttcgag gcgctggtgt tcaaggcttc
      181 ccgcggcatc gaggacatct acgagctcac ctacttccgc aaggacggca gccgcttccc
      241 cgccatcgtc tcggtcacgg ccctgcgcga tgcgcaggac gccatcatcg gctacctgct
      301 gatcggcacc gacaataccg cgcgcaagca ggcggaagag gcgctgctca aggcgggagc
      361 gctccagagc gcgatcttca acagcgccaa cttctcgagc atcgccaccg acgccaaggg
      421 cgtcatccag atcttcaacg tcggcgccga gcgcatgctg ggctacaccg ccgccgaggt
      481 catgaacaag atcactcccg ccgacatctc cgatccgcag gaagtgatcg cgcgcgccaa
      541 gacattgagc ctggaactcg caaccccgat tgcgccgggt ttcgaggcac tggtgttcaa
      601 ggcatcccgc ggcatcgagg atatctacga gctgacctat atccgcaagg acggcagccg
      661 tttccccgcc atcgtctcgg tcacggccct gcgcgaccag cgggacggga tcatcggcta
      721 cctgctgatc ggcaccgaca acagcgcccg caaggaagtc gaagcggcgc aggcgctgct
      781 cgatcagcgg ctgcgcgacc agcagttcta tacccgctcc ctgatcgaat cgaatatcga
      841 cgcgctgatg acgaccgacc cgcagggcat catctcggac gtcaaccagc agatgatcgc
      901 gctcaccgga cgcacgcgcg acgagctgat cggcgcgccc tgcaagactt tctttaccga
      961 cccggcccgc gccgaagccg cgatcaagcg cgtcctcacc gagaacaagg tgagcaacta
     1021 tgagctgacc gtgcttgcgc agaatggcga ggaaacggtg gtgtcctaca acgccgcgac
     1081 cttccacaac cgagaccgca agctgcaggg cgtattcgcg gcggcgcgcg acgtgaccga
     1141 gttgaagcgc ttcgagcgca cgctgcagga gaaaaatgtc gagctggagc acgccagcag
     1201 catgaagtcg gagttcctcg ccaccatgtc gcatgaattg cgcacgccgc tgaacgccat
     1261 catcggcttt tccgaggcac tcaaggatgg cctgatcggc ccgatgagcg acacccagca
     1321 ggaacacgtc ggcgatatct tcaccagcgg gcagcacctg ctgtccctga tcaacgacat
     1381 cctggatttg tcgaaggtcg aagccggcat gatggtcctc gagctggagc ccgtggacct
     1441 gaaaagcctc ctgtcgaaca gcctgtcgat cgtcagggaa aaagcggcgg cccagcgcat
     1501 ccagatcgag ctcgaagccg gcgaggacct gggccagccc cagctggaca tgcgcaagac
     1561 caagcagatt gtctacaacc tgctgtcgaa tgccgtcaaa ttcagcgcca ccggcgggcg
     1621 cgtgatcctg cgcgcgcggc gcgtgccgcg cggcgccgtg ggcacgctgc ccggcgactg
     1681 gccggtgcac ggtttccccc tggccgacaa tgagtatgcc gaatttcttg aaatatgcgt
     1741 gcgcgacagc ggcatcggcc tttcccggga aaacatggcg aaattgttcc aggctttcag
     1801 ccagatcgac agcagcctcg cgcgcaaatt cgagggcacc ggcctcggcc tggcgatggt
     1861 caagcaactg gccgagctgc acgggggcac cgtagcggtg gcgagcgcgg agggcgaagg
     1921 tgcgcgcttt gccgcctggc tgccgctgcg cgcgccggcg gaagccgcaa tcggcctgtc
     1981 gcaaagcgcg cgccccactc cggccggcga cgccggcgca ggggccccgg cggcagtcga
     2041 gacccgggtg cgtaatgcac tggtgatcga cgacgacgac cgggcggctg acctgcttcg
     2101 cctgtatctc gaagctgagg gattcaccgt cctgcgcgcc gtcagcgccg aggacgccct
     2161 tctgctggtg ccccggcaac tccttgccct gatcacgctg gatcttcaga tgtatggcat
     2221 g
//
LOCUS       DBNGSG010000427        11821 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1157388, whole genome shotgun sequence.
ACCESSION   DBNGSG010000427 DBNGSG010000000
VERSION     DBNGSG010000427.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 11821)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11821)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11821
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1157388"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="AC158Q_11125"
     CDS             <1..902
                     /locus_tag="AC158Q_11125"
                     /inference="COORDINATES: protein motif:HMM:NF023592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau C-terminal
                     domain-containing protein"
                     /protein_id="HIR3340369.1"
                     /translation="LDQAIAHGGGRVAQESVRAMLGVVGQDHLYAILRALAQQDGAAL
                     MAEAERMEALSLSFESALQDLASLLHRISLAQLAPGALAEDDPGHGTIEELAGLLAPE
                     DLQLYYQIAIQGRGDLGLAPDERAGFSMTLLRMLAFAPAGQGVPAPQLRAAPQRATGT
                     LPPAIASESKKNPGDTAWPELVDQLGLTAMALELAKHCELIGRDGSRIELRIAQSHQR
                     LLNGPFQDRLKSALEQHYGTKLRLSIQVAGDAGGSPAALADRDKRQRQAQAIAAIEQD
                     PFVRELVDNFDARIVGSTIKPVQ"
     gene            934..1257
                     /locus_tag="AC158Q_11130"
     CDS             934..1257
                     /locus_tag="AC158Q_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004550340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HIR3340370.1"
                     /translation="MKGGIGNLMKQAQLMQENVKKLQEQLAVMEVEGQSGSGLVKVIM
                     TGRHEVKRVSIDPGLLAEDREMLEDLVAAAFNDAARKVDATTQEKMSAATAGLPLPPG
                     LKLPF"
     gene            1365..1961
                     /gene="recR"
                     /locus_tag="AC158Q_11135"
     CDS             1365..1961
                     /gene="recR"
                     /locus_tag="AC158Q_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HIR3340371.1"
                     /translation="MKTSSSLEDLIAALRCLPGVGPKSAQRMAYHLLQRDPSGAARLA
                     AALAQALARTRHCEKCNNYAEEAVCALCSSPRRDPALLCVVEAPGDLLMMEQAQCYQG
                     LYFVLMGVLSPLDGIGPREIHLDRLIRRAADGVVREVILATNFTVEGEATAHYVGEAL
                     RARGLKVTRIARGLPVGGELEHVDSGTLAQAVLERREA"
     gene            1967..2803
                     /locus_tag="AC158Q_11140"
     CDS             1967..2803
                     /locus_tag="AC158Q_11140"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIR3340372.1"
                     /translation="MKDPRTPASGAATYKLQKALAQAGLGSRRDMEELVRAGHVTVNG
                     APATIGARVSPGDIIQVNGRTIGRSADSTPPRVLLYYKPEGEIVSRDDPEGRPSVFDR
                     LPRPRGAKWLAVGRLDFNTSGLLILTTSGELANRMMHPRYELEREYAVRVQGRLSADQ
                     QAKLLSGLRLDDGMARCEAVHDRGGEGANHWYHIVIREGRNREVRRLFEALRLRVSRL
                     MRVRFGSVSLPRSLRRGQYAELTRPEVRQLLASLGLETPSAATAPGARSARPRHPASR
                     AR"
     gene            complement(2736..3635)
                     /gene="prmB"
                     /locus_tag="AC158Q_11145"
     CDS             complement(2736..3635)
                     /gene="prmB"
                     /locus_tag="AC158Q_11145"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIR3340373.1"
                     /translation="MSRRAFHAAGRWTVGGLIAEGARRFRAARLEFGHGTSRARDEAA
                     YLTLHALKLPLDAKPTTLARYVGRRQAQRALNLFDRRVRSRRPAAYLTREAWLGGCRF
                     YVDERVLVPRSHIAGLLIERLVPWIPDASRIGTALDLCTGSGCLAILLARAFRRASID
                     AADISPAALVVARRNVRDYRLARRIRLVESDLFSALGGKTYDLIVSNPPYVTAAAMRK
                     LPPEYRREPRLALAGGEDGLDAVRTIMQQAAAYLNPGGTLVMEVGSGRRRLERAYPRV
                     AFTWPETPGGGDVLIVHREQLPR"
     gene            complement(3632..4792)
                     /locus_tag="AC158Q_11150"
     CDS             complement(3632..4792)
                     /locus_tag="AC158Q_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HIR3340374.1"
                     /translation="MFVTPLFKLMADKQASDMFFTAGAPIQIKINGVVMPINSQLLGP
                     EQCKKICYELMTEAQVKEFESLHEMNFAQSAGELGNFRVNIFRQRNTVAMVIRFVKPD
                     VPAFDSLGLPPILKEVIMEKLGLILVVGSTGSGKSTTMASMIDYRNSNRTGHILTVED
                     PVEFAFKHKKSIVNQREVRVDTQSYSNALISAMREAPDLIMIGEIRDQPTLQSCLLFA
                     QTGHLCMSTLHANNSYNALNRIINFFPREARDSLLADLATALRAVISQRLIRTTDGRR
                     IPAVEVLLNTKHIQDLITKGDLSLIKDALEQSLSPGSQSFEQALFYLINSGKVSVDEA
                     MAHSDSPTNLHWLLNNAPKSTGAPPTSAQSAAGAPKQPDDISHIKLNLDALG"
     gene            complement(4927..5748)
                     /gene="dapD"
                     /locus_tag="AC158Q_11155"
     CDS             complement(4927..5748)
                     /gene="dapD"
                     /locus_tag="AC158Q_11155"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HIR3340375.1"
                     /translation="MSELQGTIDQAWENRAELKPGSAPATVGDAVGQVLARLDAGTLR
                     VAERISGAWVVHQWVKKAVLLSFRLEDNLPMAGAATQYYDKVPSKFAHYSREDFARGG
                     FRVVPPAMVRRGAYIAKNVVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVVVEEGSVLSMGVFIGASTRIYNRQ
                     TGEVLYGRVPAGSVVVPGSLPAADGRCSLYCAVIVKQVDARTRAKTSINDLLRSE"
     gene            complement(5793..6986)
                     /gene="dapC"
                     /locus_tag="AC158Q_11160"
     CDS             complement(5793..6986)
                     /gene="dapC"
                     /locus_tag="AC158Q_11160"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 -
                     succinyldiaminopimelate:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HIR3340376.1"
                     /translation="MNPDLELLQTYPFQKLHALLDGVRPDPSRKLIPLYIGEPKHPTP
                     AFIKQALIDNLDGMASYPLTLGTAELRGAIAAWLIRRYRLDSVDPATEVLPVTGSREA
                     LFAIAQTVVDATRPDPVVVCPNPFYQIYEGAALLAGATPHYLNSLPANGYAFDFDQLP
                     DATWRRAQLIYVCSPGNPTGYVMPLADWERLFALSDHYGFVIASDECYSEIYFDEARP
                     PLGALQAARQLGRKNYPRLVVFSSLSKRSNVPGMRSGFVAGDASILKQYLLYRTYQGC
                     AMSPTVQQASITAWKDEKHVVENRRLYREKFAAALEILPAVLEAEMPEAAFYLWLRTP
                     ISDTEFARRLYQQQAVTVLPGSYLAREAAGVNPGTNRVRIALVSSVEECVESAQRIRA
                     FAESL"
     gene            complement(7075..7494)
                     /gene="queF"
                     /locus_tag="AC158Q_11165"
     CDS             complement(7075..7494)
                     /gene="queF"
                     /locus_tag="AC158Q_11165"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008812003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046857 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors, with NAD or NADP
                     as acceptor [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HIR3340377.1"
                     /translation="MPSKPSRKLETFANPQPARDYRIHMEIPEFTCLCPRTGQPDFAT
                     LVLDYVPDRLCIELKSLKLYVWSFRNEGAFHEAVTNRILGDLARATRPRFMRLTARFN
                     VRGGIFTTIVAEHAKPGWKPRRAVDLSNLPASSSTRG"
     gene            7575..11099
                     /gene="smc"
                     /locus_tag="AC158Q_11170"
     CDS             7575..11099
                     /gene="smc"
                     /locus_tag="AC158Q_11170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HIR3340378.1"
                     /translation="MRLKQINLAGFKSFVDPTHIPVPGQLVGIVGPNGCGKSNVIDAV
                     RWVLGETSAKHLRGDTMQDILFNGSGQRQPVNRSSVELIFDNSLGRAGGQWSSYAEIS
                     VKRVLARDDEATSYYINNVHVRRRDVADIFLGTGLGSRAYAIIEQGMISRVIEARPED
                     LRVFLEEAAGVSKYRARRHETELRLKDTRENLQRVEDVRLELVKQLEHLQSQAEIASR
                     YHELQGRLRVSQNLLSLLKKQEAASQRARHIREVERLTLELEAETAKLRDTEKRLEDL
                     RAQHYRGSDAVHAAQGAFYEINAETARLEQELAHLRDNRQRVERDLADLRAQAVRGRE
                     QLAAAQAGLAGLQTERARAEEGVAACEAALRIENDKLPLAEEAFKATRNRYEELQRSL
                     ALAEQALGVEQTKRTHARQTTEQLRLRAQRLQEERVGLQPADAPRLQQLRGEMAALDQ
                     ELRGLRTSMEAEEERLPQSERFLRDRDTAQEQSGQRLAGLEAQLQALVQLQERLARGA
                     NTKDWLETHGLGKAPRLWQQISIESGWEDALEAALRERLNAIALERMEQAGHWTSDPP
                     PGRITVFAPSGAAATPAAALSGCEALRRYVTCNDARLQAVVEDWLHQVYVINDTAAAL
                     AAREGLPAGAMLVTREGHIFTRHTMSFHAPDSELHGVLSRQREIEQLGGTLTQARAAQ
                     EQLREAAAAAEREVAQKRAGLDELRDAISDRQQRHHALQLEALQLTERGQRVTQRMER
                     IAGELAEIEAQSVRVIEAGEAAERRVRELQAEVEQQQGQLQLASDLYRRAENILDLQR
                     NALQQARESAQKAVFTKQSCDNKIIEIQNSIKAISDSTLHLDATLAARHQELAGFDET
                     SLQQRLQQALAARAEREQALAQTRDALEGMETGLKASDEERHAVEQKLEPLRERIAEM
                     RLKEQEARLNEEQYAQQLADAGAREDEVAQQLEKGMRPSTLNNEISRLNEEIKGLGAV
                     NLAALEELRTATERKDYLDAQSRDLNEAMTTLEDAIRRIDRETRERLQHTFDEVNRHF
                     SEMFPALFGGGSAKLVLTGAEILDAGVLVQAQPPGKKNTTIHLLSGGEKALTALSLVF
                     SMFQLNPAPFCLLDEVDAPLDDHNTGRFCELVKKMSKQSQFLFISHNKITMEIAEQLL
                     GITMQEPGVSRVVAVDIEEAMKLSEAAA"
     gene            11107..>11821
                     /locus_tag="AC158Q_11175"
     CDS             11107..>11821
                     /locus_tag="AC158Q_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340379.1"
                     /translation="MSDLQWSLLVIGAVVVAAVYLYNWLQERRFRGRMQQAFGAAHED
                     VLLKAGVESALTDGRLEPQLVPPEPDRGERHAARAGPDADIENNFDAVLDYVAEIDAD
                     APIADAVIAELTNKIASCGKPVHVAGLDASNEIWEDALRGRGGRFRRLRLSLQLVNRA
                     GPVNPAQLAIFCDAVKSCADKIPAPANCPDTQAALKHARELDAFCADVDVAIGFNVVA
                     AEGSSFPGTRIRAVAEAAGF"
BASE COUNT         2195 a         3650 c         3993 g         1983 t
ORIGIN      
        1 tgctcgacca ggcgattgcc cacggcgggg ggcgcgtcgc gcaggaatcg gtacgcgcca
       61 tgctcggcgt ggtcgggcag gatcacctct atgcgatcct gcgtgcgctg gcgcaacagg
      121 acggtgccgc cctgatggcg gaagccgagc gcatggaagc gctcagcctg tcgttcgagt
      181 ccgcgcttca ggacctggca tcgctcctgc accggatttc gctcgcgcaa ttggcgccgg
      241 gcgccctcgc cgaggacgac cccggccacg gcacgatcga ggaactggcc gggctccttg
      301 ctccagagga cctgcagctc tactaccaga ttgcgataca aggacgcggc gatctcggcc
      361 ttgcgcccga cgagcgtgca ggtttctcga tgacgttgct gaggatgctt gcgtttgccc
      421 cggcgggaca gggcgtcccg gccccgcagc tacgggccgc gccgcagcgc gcgaccggca
      481 cactgccgcc cgcaatcgcc tccgaatcaa aaaaaaaccc cggtgacacc gcctggccgg
      541 agctggtcga tcagctcggc ttgacggcca tggcgctcga actcgccaag cattgcgaac
      601 tgattggccg cgacggatcc cgcatcgaac tgagaatcgc ccaaagccac cagcgcctgc
      661 tcaacggtcc gttccaggac cggttaaagt cggcgctgga gcaacattac ggcacgaagc
      721 tgagactttc cattcaggtg gcgggagacg cgggcggttc gccggcagcg ttggccgatc
      781 gggacaagcg gcagcggcag gcacaggcca tagcggccat cgagcaggac cccttcgtgc
      841 gcgagctggt ggacaacttc gacgcccgga tagtggggtc tacgatcaaa ccggttcaat
      901 gagcgatgca atgaatcgac aggagacgca cgcatgaagg gcggtatcgg aaacctgatg
      961 aagcaggcgc aactcatgca ggagaacgtg aagaagctgc aggagcagct ggccgtcatg
     1021 gaagtcgagg gccagtcggg ctctggcctg gtgaaggtca tcatgaccgg gcgtcacgaa
     1081 gtcaagcgcg tgtccatcga tccggggctt ctggccgaag acagggagat gctggaagac
     1141 ctcgtcgcgg cggctttcaa tgatgcggcg cgcaaggtcg atgccacgac acaggagaag
     1201 atgtccgccg caacggcagg attgcctctg ccaccaggat taaaactgcc gttttaatcc
     1261 ttgatgagga atgatgagtt cggaatgatg aattcgcctt cgaatagccc gcttttgttc
     1321 atcattcatc attcatcgtt catcatttat cagttcttcg agcgatgaag acctcttcat
     1381 cgctggagga cctgatcgcc gcgctccgct gcctgcccgg tgtcgggccc aaatccgctc
     1441 agcgcatggc ttatcacctg ctgcagcgcg atccttcggg agcggcacgg ctggccgcag
     1501 cactcgcgca ggcgctcgcc aggacccggc actgcgaaaa gtgcaacaac tatgccgagg
     1561 aggccgtttg cgccttgtgc agttcaccgc ggcgcgatcc cgccttgctg tgcgtggtgg
     1621 aggcgccagg cgatctgctc atgatggaac aggcgcaatg ctaccagggc ctgtactttg
     1681 tcctgatggg cgtgctgtcg cctctcgacg gcatcgggcc cagggagatc catctcgatc
     1741 gcctgatccg gcgcgccgcc gacggtgtgg tacgcgaagt catcctcgcc acgaatttca
     1801 ccgtggaggg agaggcgacc gcgcattacg tgggcgaggc attgcgtgcc cgcggattga
     1861 aggtgactcg catcgcgcgc ggcctgccgg tcggcggcga actcgaacac gtggatagcg
     1921 gaacactcgc ccaggccgtt ctggaacggc gcgaggcctg accgccatga aagaccccag
     1981 aaccccagct tccggcgcag cgacgtacaa gctgcagaag gcattggccc aggccggcct
     2041 gggatcccgc cgcgatatgg aggaactggt gcgtgccggc cacgtgaccg tcaatggggc
     2101 tcccgccacc atcggcgccc gcgtaagccc gggcgatatc atccaggtca atggccgcac
     2161 catcggccgt tccgcggaca gcactccgcc gcgggtgctg ctctactaca agcccgaggg
     2221 cgagatcgtg agccgcgacg accccgaggg gcgccccagc gtgttcgatc gcctgccgcg
     2281 tccgcgcggc gcgaaatggc tggcggtggg tcgcctcgac ttcaacacca gcggtctgct
     2341 gattctcacc acgtcaggcg agctcgccaa ccgcatgatg catccgcgct acgagctgga
     2401 gcgtgagtac gcggtgcgcg tgcagggccg gctttccgcg gaccagcagg cgaagctgct
     2461 gtccgggctg cggctcgatg acggcatggc ccgatgtgag gccgttcatg accggggcgg
     2521 cgaaggcgcc aatcactggt accacattgt catccgcgaa gggcgcaatc gcgaggtgcg
     2581 ccgcctgttc gaggcgctgc gtctgagagt cagccggctg atgcgcgtgc gctttggcag
     2641 tgtgagcctg ccgcggagcc tgaggcgcgg gcaatacgcc gagttgacgc gcccggaggt
     2701 ccggcagctg ctcgcatcgc tggggctgga gacgcctagc gcggcaactg ctcccggtgc
     2761 acgatcagca cgtccccgcc acccggcgtc tcgggccagg tgaacgccac ccgcgggtat
     2821 gcccgctcga ggcggcggcg cccgctgccc acctccatca ccagcgtgcc gccgggattg
     2881 agataggcgg cagcctgttg cattatcgtg cgcactgcgt cgaggccgtc ttcgccgccc
     2941 gccaacgcca atcggggttc gcggcgatac tcaggcggta acttccgcat cgcggcagcc
     3001 gtgacatacg gcgggttgga tacgatcaag tcataagttt tcccgcccag tgcggaaaac
     3061 aggtccgatt ccacgagtcg gatgcgtcgc gcgaggcggt aatccctgac gttgcgccgt
     3121 gccaccacca gcgccgccgg cgaaatgtcg gcggcatcta tgctcgcccg gcgaaatgca
     3181 cgcgccagca gtatggcgag gcaacccgac cccgtgcaga ggtccagcgc cgttccaatc
     3241 ctgctggcgt cgggaatcca gggaacgagg cgctcgatca gcaatcccgc gatatgcgaa
     3301 cggggaacga ggaccctttc gtcgacgtag aagcggcatc cgcccagcca ggcttcccgc
     3361 gtcaggtagg cggctggcct gcggctgcga acgcggcgat cgaagagatt cagggcgcgc
     3421 tgcgcctgcc gccggccgac ataccgtgcc agggtggtcg gctttgcatc gagcgggagt
     3481 ttcagcgcgt gcagggtgag ataggccgcc tcgtcgcggg cgcgggaggt gccatggccg
     3541 aattcgaggc gggcggcacg gaagcggcgc gcgccctctg ctatcagccc gcctacggtc
     3601 cagcggccgg ccgcatggaa tgcgcgtcgg ctcaaccgag agcgtcgaga ttcagcttga
     3661 tgtgggagat gtcgtcaggc tgcttggggg cgcccgccgc ggactgggcg gaagtgggtg
     3721 gggcgccggt ggatttggga gcattgttca gcaaccagtg caggttggtc ggggaatccg
     3781 agtgcgccat cgcttcgtcc acgctcacct tgccgctgtt gatcaggtag aacagcgcct
     3841 gttcgaagga ctgggagccg ggcgagaggc tctgctccag ggcgtccttg atcaggctca
     3901 aatcgccttt cgtgatcagg tcctggatat gcttggtgtt gagcagcact tccacggcgg
     3961 gaatgcgcct gccatccgtg gtgcgtatca ggcgctggga gatcacggcg cgcagcgcgg
     4021 tcgccagatc ggcgagcagt gaatcgcgcg cttcgcgcgg gaagaagttg atgatgcggt
     4081 tcagcgcgtt gtagctgttg ttggcgtgga gcgtggacat gcacaggtgc ccggtctgcg
     4141 cgaacagcag gcagctctgc aatgtcggct ggtcgcgaat ctcgccgatc atgatgagat
     4201 ccggcgcttc gcgcatcgcg ctgatcagcg cattcgagta gctttgcgtg tcgacgcgga
     4261 cttcccgctg gttgacgatg gatttcttgt gcttgaaagc gaattccacc gggtcttcga
     4321 cggtgaggat gtggccggtc cggttgctgt tgcggtaatc gatcatggag gccatggtgg
     4381 tggacttgcc cgagccggtc gatcccacca cgaggatcag ccccagtttt tccatgatca
     4441 cttccttcag gatcggcggc agtcccaggg aatcgaacgc gggcacatcg ggcttgacga
     4501 agcggatcac catcgccacc gtgttcctct ggcggaagat gttcacccgg aagttgccga
     4561 gttcgcccgc gctctgtgcg aagttcattt catggaggga ttcgaactcc ttcacctgcg
     4621 cctcggtcat cagctcgtag cagatcttct tgcactgctc ggggccgagc agctggctgt
     4681 tgatcggcat gaccacgccg ttgatcttga tctggattgg tgccccggcc gtgaaaaaca
     4741 tgtcggaggc ctgcttgtcg gccatcagct tgaacagcgg cgtcacgaac atgattgtcc
     4801 cccgtgcgtg attaatggcg tggaatcagt ctgttggagc tcatctgctg cgccggttgc
     4861 gagcgcagga cagggacccc ttcctgccgg gtattgtgcc aactccggac cgcgttgcct
     4921 agcgttctac tcgctgcgca aaagatcgtt gatgctggtc ttggcgcggg ttctggcgtc
     4981 cacctgcttg acgatcacgg cgcagtagag gctgcacctg ccgtccgctg caggcaggct
     5041 gcccggcacc acgaccgaac ccgccggcac gcgcccgtag agaacctccc cggtttgccg
     5101 gttgtagatg cgggtgctgg cgccgatgaa tacccccatc gagagcacgg agccttcctc
     5161 gaccacgacg ccctccacga cttcggagcg cgccccgatg aagcagttgt cctcgatgat
     5221 ggtgggattc gcctgcaagg gttccagcac accgcctatg cccacgccgc ccgacagatg
     5281 cacgttcttg ccgatctggg cgcaggaacc gacggtggcc caggtgtcca ccatggtgcc
     5341 ttcatcgacg taggcgccga tgttgacgta agagggcatc agcaccacgt ttttcgcaat
     5401 ataggcgccg cgcctcacca tggccggcgg caccacgcga aagccgccgc gcgcgaaatc
     5461 ctcgcgcgag taatgggcga acttgctcgg aaccttgtcg taatactggg tcgccgcgcc
     5521 cgccatcggc aagttgtcct cgagacggaa ggacagcaat acggctttct tgacccactg
     5581 gtgcacgacc cacgcgccgc tgattctctc cgcgacacgg agggtgccgg catcgaggcg
     5641 ggcgagcacc tggccgaccg cgtcgcccac tgtcgcgggg gcgctgccgg gtttcagctc
     5701 ggcgcggttt tcccacgcct gatcgatggt accttgcagc tcgctcatga cgggattcta
     5761 cctccggtta aaacggaccg cttgcgcaac cgtcacaggg attcggcaaa ggcgcggatg
     5821 cgctgcgccg actccacgca ttcctcgacc gacgaaacca aggcgatgcg cacccggttg
     5881 gttccggggt taactccggc ggcttcgcgt gcaaggtagc ttccaggcag caccgtgacg
     5941 gcctgttgct ggtagagccg gcgcgcgaac tcggtgtcgg atatcggcgt cctcagccac
     6001 aggtagaacg cggcctcggg catctcggct tcgagaaccg cgggcagtat ctccagcgcc
     6061 gcagcgaatt tctcccggta caggcgccgg ttctcgacca cgtgcttctc atccttccat
     6121 gcggttatgc tcgcctgctg caccgtgggg ctcatcgcgc agccctgata ggtgcggtag
     6181 agcaggtact gcttgagtat ggaagcatcg cccgcgacga aaccggaacg catgccgggc
     6241 acgttcgagc gcttggacag actcgagaac accaccaggc gcggatagtt cttgcggccg
     6301 agttgccggg ccgcctgcaa cgcgcccaga ggggggcggg cctcgtcgaa gtatatttcg
     6361 gaatagcact cgtccgatgc aatcacgaac ccgtaatggt cggacagcgc gaacagccgc
     6421 tcccagtcgg caagcggcat gacataaccc gtcgggttgc cgggcgaaca gacgtagatc
     6481 agctgcgcgc ggcgccatgt cgcatccggc aactggtcga aatcgaaggc ataaccattc
     6541 gcgggaaggc tgttcagata gtgcggcgtg gccccagcca gcaacgccgc gccttcgtag
     6601 atctggtaga agggattggg gcagaccacg accgggtcag ggcgcgtcgc gtccactacc
     6661 gtctgcgcga tggcaaacag ggcctcgcgg cttccggtga ccggcagcac ctcggttgcc
     6721 ggatccacgc tatcgagccg gtaacgccgt atcagccacg cggcgatggc gccgcgcaac
     6781 tccgcggttc ccagcgtcaa ggggtaactt gccatcccgt ccagattgtc gatcaaagcc
     6841 tgtttgatga atgccggcgt cgggtgcttc ggctcgccga tataaagggg aatcagcttg
     6901 cgcgacggat ccggccgcac gccgtcgagc agcgcgtgaa gcttctggaa cgggtacgtt
     6961 tgcagtagtt cgagatcggg attcatgtcg cctgcgccgc cgggagctgg gcaaggccca
     7021 gaattataaa ggctttctcc ggccggcacc cgggaaagcg ttgtctcgcg gcgatcagcc
     7081 gcgcgtgctg gacgaagcgg gaaggttcga aagatccacc gcacggcgcg gtttccagcc
     7141 cggcttggcg tgctcggcga cgatggtcgt gaaaatgccg ccgcggacgt tgaatcgcgc
     7201 agtgagccgc atgaaacgcg ggcgcgtggc ccgcgcgaga tcgccgagga tgcggttggt
     7261 aacagcctcg tggaacgcgc cttcgttgcg gaaagaccag acgtacagct tgaggctttt
     7321 caattcgatg cagagccggt ccggtacgta gtcgagcacc agggtcgcga agtcaggctg
     7381 gccggtgcgc gggcacaggc aggtaaactc cggtatttcc atgtgaatcc ggtagtcgcg
     7441 cgccggctgg gggttggcga aggtttccag cttgcggctc ggtttgcttg gcattatcgc
     7501 ggcggcccga tatttcggta aaatagcgaa gataaatagt ctgcggatta taacctccac
     7561 aataagacct taaattgaga ctcaaacaga tcaatctcgc aggcttcaag tctttcgtag
     7621 atcccactca cattcccgtc cccggacaac tggtaggcat cgtcggcccc aacggctgcg
     7681 gcaagtccaa cgtcattgac gccgtgcgct gggtgctggg agaaacttcc gcgaagcacc
     7741 tgcgcggtga caccatgcag gacatccttt tcaacggttc cggccagcgc cagccggtga
     7801 accgctccag cgttgaattg atattcgata acagcctcgg ccgggcgggt gggcaatggt
     7861 cgagctacgc cgagatctcg gtgaagcgcg tgctggcgcg cgacgacgag gccacgtcgt
     7921 actacatcaa caacgttcac gtgcgccggc gcgacgtcgc cgacatcttc ctcggcacgg
     7981 gccttggcag ccgcgcctac gccatcatcg agcagggcat gatttcgcgg gtgatcgaag
     8041 ccaggcccga ggatctgcgc gtgtttctgg aggaggccgc gggggtatcg aagtaccgcg
     8101 cgcgccgcca cgaaaccgag ctgaggctca aggacacgcg cgagaacctg cagcgcgtgg
     8161 aagacgtgcg cctggaactg gtgaagcagc tcgagcacct gcaatcccag gcggagatcg
     8221 cgagccgcta ccacgagttg cagggccggc tgcgggtttc gcagaacctg ttgtcactgc
     8281 tgaagaagca ggaagccgcc tcccagcgcg cgcggcacat cagggaagtc gagcgcctca
     8341 ccctggaact ggaggcggag actgcgaagt tgcgggacac cgaaaagcgc ctcgaggact
     8401 tgcgcgcgca gcactaccgc ggcagcgatg ccgtgcatgc ggcgcagggc gccttctacg
     8461 agatcaatgc cgagaccgcc cggctcgagc aggagctcgc ccacctgcgc gataaccgcc
     8521 agcgggtgga acgcgacctg gccgacttgc gcgcgcaggc cgtgcgcggg cgggaacagc
     8581 tcgcggcggc gcaagccggc ctggccggct tgcagacgga gcgcgcccgg gcggaagaag
     8641 gcgttgcggc gtgcgaagcg gcgctcagga ttgagaatga caagctgccc ctggcggagg
     8701 aggcgttcaa ggccacccgc aatcgctacg aagaattaca gcgttcactg gccctggcgg
     8761 agcaggccct gggcgtggag cagaccaaac ggactcatgc ccggcagacg accgagcagc
     8821 tgcggctgcg cgcgcagcgc ttgcaggagg agcgcgtggg gctgcagcct gccgatgccc
     8881 cgcggctcca gcagttgcgc ggcgaaatgg cggcgctcga ccaggaactg cgcggcctgc
     8941 gcaccagcat ggaggccgaa gaggaacgct tgccgcaatc cgagaggttt ctgcgcgaca
     9001 gagacacggc gcaggagcaa tcgggtcaac gcctggccgg attggaagcg cagcttcagg
     9061 cgctcgtcca gttgcaggag cgcttggcac ggggcgccaa caccaaggac tggctggaga
     9121 cgcacggcct cggcaaggca ccccggttgt ggcagcagat cagcatcgag tccggctggg
     9181 aggacgcgct ggaagcggcg ttgcgcgagc gcctgaacgc gatcgccctc gagcggatgg
     9241 agcaggccgg gcactggacg agcgatccgc cgccgggaag gatcacggtg ttcgcgcctt
     9301 ccggcgcggc cgcgacgccg gccgcggcgc tttccggttg cgaggcgctg cgccgttacg
     9361 tcacctgcaa cgacgcgcgg ctgcaagccg tggtcgagga ctggctccac caggtgtacg
     9421 taatcaacga tacggccgca gcgctggcgg cccgtgaggg cctgccggcc ggtgcgatgc
     9481 tggtcacgcg ggaaggccat atcttcaccc gccacacgat gagttttcat gcgccggaca
     9541 gcgaattgca cggcgtgttg agccggcagc gcgagatcga gcagttgggc gggaccttga
     9601 ctcaggcgcg ggccgcacag gagcagctgc gggaagctgc tgctgccgcg gaacgggaag
     9661 tggcgcagaa gcgcgcgggg ctggatgaac tgcgcgatgc gatcagcgac cggcaacagc
     9721 ggcatcacgc gctgcagctg gaagcgctac agttgaccga gcggggccag cgcgtcactc
     9781 agcggatgga aaggattgcc ggagaactcg ccgagatcga ggcccaatcg gtcagggtaa
     9841 tcgaggcggg agaggctgcg gagcgacgcg tccgcgagct gcaagcggag gtggagcagc
     9901 agcagggcca gctccagctc gccagcgacc tctaccggcg cgcagagaac attctcgatc
     9961 tgcaacgcaa tgccttgcag caggcgcgcg aatcggccca gaaagccgtg tttacgaagc
    10021 aatcatgtga taataaaatt atagagatac agaactctat taaagctatt tcggacagca
    10081 cgctgcatct cgatgccaca ctggccgccc gtcaccagga gctggccggt tttgacgaga
    10141 cttcgctgca gcagcgcctg caacaggcgc tggcggcgcg cgcggagcgt gagcaggcac
    10201 tggcgcaaac acgcgacgcg ctggaaggaa tggaaacggg gctcaaggca agcgacgagg
    10261 aacggcatgc ggtggagcag aagctggagc cgctgcgcga gcggatagcc gagatgcgtt
    10321 tgaaggaaca ggaggcgcgg ctcaacgagg aacagtatgc gcagcagctg gcggacgccg
    10381 gcgcgcgcga ggacgaggtg gcgcaacagc tggaaaaagg catgcggccc agcaccctca
    10441 acaacgagat ctcccgtctg aacgaggaga tcaaggggct gggtgcggtc aacctggcgg
    10501 cactggagga gttgcgaacc gcgaccgagc gcaaggacta ccttgatgcg cagtcgcgcg
    10561 acctgaacga ggcgatgacg acgctggaag atgccatccg gcgcatcgac cgcgagacgc
    10621 gggagcggct gcagcatacc ttcgacgagg tcaatcgcca tttcagcgaa atgttcccgg
    10681 cgctgttcgg cggcggaagc gcgaaactgg tgctgaccgg cgcggagatc ctggatgcgg
    10741 gcgtgctggt gcaggcgcag cctcctggca agaagaacac caccatccac ctgctgtcgg
    10801 gaggagagaa ggcgttgacc gcgctgtccc tggtgttctc gatgtttcag ttgaatcccg
    10861 cgccgttctg cctgctcgac gaggtggacg cgccgctcga cgatcacaac accgggcgct
    10921 tttgcgagct ggtgaagaag atgtcgaagc aatcgcaatt cctgttcatc agccacaaca
    10981 agatcaccat ggaaatcgcc gaacagctgc tcggcatcac catgcaggaa cccggcgtct
    11041 cgcgcgtggt ggcggtcgat atcgaagagg cgatgaagct gagcgaagcc gcggcctgag
    11101 gtggtcatga gcgacctgca gtggagcctg ctcgtcatcg gagcggtggt ggtcgccgcg
    11161 gtctatctct acaactggct gcaggagcgc cgtttccgcg gccgcatgca gcaggcgttc
    11221 ggtgccgcgc acgaagatgt gctgctgaag gccggggtcg aatcggcttt gacggacggc
    11281 cggctggagc cccagctggt gccgccggaa ccggaccgcg gggaacgcca cgcggcccgt
    11341 gccggcccgg acgcagatat cgaaaataat tttgacgccg tcctcgacta cgtggcggaa
    11401 attgatgcgg atgccccgat agccgacgcg gtgatcgccg agctcaccaa caagattgct
    11461 tcctgcggca agcccgtgca cgttgcgggt ctcgacgctt cgaatgagat ttgggaggat
    11521 gccctgcgcg ggcggggcgg gcgttttcgc cgcctgcgcc tatcgcttca gctggtgaac
    11581 cgtgctggac cggtcaaccc ggcccagttg gcgattttct gcgatgcggt aaagagttgc
    11641 gccgacaaga taccggcgcc ggcgaactgc ccggatacgc aggccgccct gaagcacgcg
    11701 cgcgagctcg acgcgttctg cgccgacgtc gacgtcgcga tcggcttcaa tgtcgttgcg
    11761 gcggaaggtt cgtcattccc gggcacccgc atacgcgccg tggcggaggc cgccggcttc
    11821 a
//
LOCUS       DBNGSG010000428         2305 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_138656, whole genome shotgun sequence.
ACCESSION   DBNGSG010000428 DBNGSG010000000
VERSION     DBNGSG010000428.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_138656"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="AC158Q_11180"
     CDS             complement(<1..506)
                     /locus_tag="AC158Q_11180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3340380.1"
                     /translation="MDFSLAPQQDELKASVARFAEAELNADLARREKEQAFNRAGWRR
                     CAEMGIQGLPVPARYGGKEADPVTTLAALEGLGYGCRDNGLNFSINAHLWGCSYPIVV
                     FGSEPQKERFLRRLCTGESIGALAISENEAGSDAYSLKATAERRGDHYLLNGHKIFVT
                     NGPIADLVL"
     gene            complement(603..1997)
                     /locus_tag="AC158Q_11185"
     CDS             complement(603..1997)
                     /locus_tag="AC158Q_11185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3340381.1"
                     /translation="MTSRKKVDALVIGGGPAGATVAMRLLAKGIRPLVVERERFPRYH
                     VGESMTGECGFIVRELGLEARMAANGHPVKHGVCVFGAKGTSDWWVPVMQRTEDGVLH
                     EQVTWQVRRSVFDAMLLDEALQRGADLVHGRAVAPIVSEDRSTVQGAWIRLANGPTLM
                     IEAGITIDCSGQASFLASHRITGPKYLGAYDKQIAIFSQVANYARGSGGERMHQPGNT
                     HIFYKKKYHWAWAIPIEDGITSIGVVVPAQYFRDCGETKDDFLRREMRELNKGLAERI
                     PDPELVEPAHVIPNYSFQVSKFAGPGYICVGDSHRFIDPIFSFGLFISMKEAGLAADA
                     IASYLDGKGRDSADPFHDHMVGVETGSDILEDVLDTFWENPFAFSVFVHGKYRDPLID
                     VFAGRIYDRMPNKGRDEAIAAFRRLLKRERSYRESALFSVPIGSRFHPERAPLWNSEL
                     DCAEATERWMRDTE"
BASE COUNT          390 a          721 c          759 g          435 t
ORIGIN      
        1 agcacgaggt cggcgatggg cccgttcgtc acgaatatct tgtggccgtt cagcaggtaa
       61 tgatctccgc ggcgctcggc ggtggccttc aggctgtacg cgtccgagcc cgcttcgttc
      121 tcgctgatcg ccagcgcgcc gatcgattct ccggtgcaga ggcggcgcag gaaccgttcc
      181 ttttgcggct ccgagccgaa gacgacgatc ggatagctgc agccccagag atgggcgttg
      241 atggaaaagt tcaggccgtt gtcccggcaa ccgtaaccga gcccctccag cgccgcgagg
      301 gtggtcacgg ggtcggcttc cttgccgcca tagcgggccg gaaccggcag gccctggatt
      361 cccatctccg cgcagcgccg ccagccggcc cggttaaacg cctgctcttt ctcgcggcgc
      421 gcaaggtccg cgttcagctc ggcctcggcg aaccgggcca ccgacgcctt gagctcgtct
      481 tgctgcggtg ccagagagaa atccatggtg cctgccgtga ccggagcctg gagggcttga
      541 ggatggctgt tctgaatccg ctcgatggag ccgtgttgag gcgcgtaagc gaacgggcgc
      601 cgctactcgg tatcccgcat ccagcgctcg gtggcttcgg cgcagtcgag ctcggaattc
      661 cagagtggcg cgcgctcggg gtggaaacgg gagccgatcg gcacggagaa gagggcggac
      721 tcgcggtatg accgttcgcg cttgagcagc cggcggaagg cagcgatagc ctcgtcgcgc
      781 cccttgttcg gcatgcggtc atagatgcgg cccgcgaata catcgatcag ggggtcgcgg
      841 tacttgccgt ggacaaagac cgaaaacgca aacgggttct cccagaacgt gtccaggacg
      901 tcttcaagta tgtcgctgcc ggtttccacc ccgaccatgt gatcatggaa aggatccgcc
      961 gagtcccgcc cttttccatc caggtagctg gcgatggcat cggccgccag gcccgcctct
     1021 ttcatggaaa tgaacaggcc gaaggagaaa atcgggtcga tgaagcgatg cgagtcaccc
     1081 acgcagatat agccgggacc ggcgaacttg ctgacctgga acgagtagtt gggaatcacg
     1141 tgggcgggtt cgaccagttc gggatcgggg atgcgctccg ccaggccctt gttcagctcg
     1201 cgcatttccc ggcgcaggaa gtcgtctttt gtttccccgc agtcgcggaa atattgcgcg
     1261 ggcaccacca ccccgatgct cgtgatcccg tcttcgatgg gaatcgccca cgcccagtgg
     1321 tatttcttct tgtaaaaaat atgcgtgttg ccgggctggt gcatgcgctc gccgccgctg
     1381 ccgcgggcgt aattggcgac ctgggagaaa atggcgatct gcttgtcgta ggcgcccaga
     1441 tacttcggcc cggtaattct gtggctggcg aggaaactgg cctggccgga gcagtcgatg
     1501 gtgatgcccg cttcgatcat cagggtcggg ccgttcgcga ggcggatcca ggcgccctgc
     1561 accgtggaac ggtcttcact cacgatcggc gcaaccgcgc gcccgtgaac caggtcggcc
     1621 cccctctgca gcgcttcatc gagcaacatg gcgtcgaaga cgctgcggcg cacctgccag
     1681 gtgacctgct catgcagtac cccgtcctcg gtgcgctgca tgaccggaac ccaccagtcg
     1741 ctggttccct tggcgccgaa aacgcatacg ccgtgcttga ccgggtggcc gttggcggcc
     1801 atgcgcgctt ccagacccag ctcgcggacg atgaagccgc attcgccggt catcgactcg
     1861 ccgacgtggt agcggggaaa gcgttcgcgc tcgacgacga gcggcctgat cccctttgcc
     1921 agcagccgca tggccacggt tgctcccgcg gggccgccgc cgattacgag cgcatccact
     1981 ttcttgcggg acgtcatgct gttcaagttt cccctgtgag ctcgatgccg cgaaaatcaa
     2041 tggcttgaag aaccgtgccg cggcgaaagc gtgttgtggt gtgagccacg cattttactg
     2101 caccccgggg catttccgca cgccggatgc gcgctctatt gcggggccgg ctgtcgattg
     2161 tagtgatgta atgggaatct ggacgggagt ccaggcaggc ggtcggagcg tcgggcttgc
     2221 tcagttcccc attcagaaca cccggccgtc caggatgccg aggaggtagc ggccgtagcc
     2281 gctgttcttc agcggcgcgg cgagc
//
LOCUS       DBNGSG010000429         4672 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3535171, whole genome shotgun sequence.
ACCESSION   DBNGSG010000429 DBNGSG010000000
VERSION     DBNGSG010000429.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4672)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4672
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3535171"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="AC158Q_11190"
     CDS             complement(<1..311)
                     /locus_tag="AC158Q_11190"
                     /inference="COORDINATES: protein motif:HMM:NF019603.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HIR3340382.1"
                     /translation="MQIRNIGIVSPGDMGQAIAGRLKESGMNVYTALDGRSERTKALA
                     REAGLADCGSIEKLVSTCELVISVIDPGQSPAVARNVADAMRKTGRKIAFADMNAVSP
                     QT"
     gene            527..1717
                     /locus_tag="AC158Q_11195"
     CDS             527..1717
                     /locus_tag="AC158Q_11195"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3340383.1"
                     /translation="MPAVAGRARSEMSFWLIVLVVVLNHGCLKGAKVLMSLSSIELGA
                     SPATIGLLYAMYSLLPALLSVYAGKLSDRRGFGSLATLASAGLCAGLLLPYCFAGLGT
                     LFASAAIAGASYIFYIVAIQHLFGSIGEGADRTRNYGIFAICVSVTSLVGPVTAGFSI
                     DVIGHRGSWLLIASFPAVATLALWLFSARLPVHRPHERPAGRQRLLDLLRNAPLRRVL
                     LATAFLETGMELFNFLMPIYGHSIGLSASRIGIVMGSFALAMMLVRIVMPRLARRWSE
                     ERVFSVSLFVAGSVSLVFPLVSGFVPLLAAAFVLGLGFGSGAPLSMALSYNRAPAGRT
                     GEAIGLRQTVNKGIEATVPAAFGFLSAAFGMVPVYWVGALLLGCGGWLMHGDARRKSA
                     KRNS"
     gene            1836..2534
                     /gene="pcaH"
                     /locus_tag="AC158Q_11200"
     CDS             1836..2534
                     /gene="pcaH"
                     /locus_tag="AC158Q_11200"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006163071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HIR3340384.1"
                     /translation="MKLRKLPVGVHPNLLHPAYRSTVERAPRQRPIALPPGATEQSGP
                     RFDPAVLYPHHDDLTRQVEGGEAAGQRIIVTGRVLDENGDPLPDTLVEIWQCNAAGRY
                     HHGQDNHDAPLDPYFRGGGRMLTDAAGAYRFTSIRPGAYPWRNHPNAWRPAHIHFSVF
                     GRTMLERLITQMYFPGDPLLGYDPIFNSVPTERARERLISHFDLEVTQPEWALGFHFD
                     IVLRGPEATPANDE"
     gene            2548..3096
                     /gene="pcaG"
                     /locus_tag="AC158Q_11205"
     CDS             2548..3096
                     /gene="pcaG"
                     /locus_tag="AC158Q_11205"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HIR3340385.1"
                     /translation="MAELLITPSQTIGPFFKYGLEWPGGESLFPDSAQGRRIRLGGVV
                     TDFKDEPVPDALIEFWQADAKGQFGAARAKASGGFGRVATDERGRYAINTIFPGPVAG
                     ANGRKQAPHILVVLFARGLLAQVVTRVYFEGEAANKDDPVLALCGVRASTLMAKRVAD
                     DANAYDWDISLQGQNETVFFEA"
     gene            3191..3529
                     /locus_tag="AC158Q_11210"
     CDS             3191..3529
                     /locus_tag="AC158Q_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HIR3340386.1"
                     /translation="MKDDRWIPLCGVADVAPGCGLRAETGGCVYAVFNVEGRFYVTQD
                     ECTHGPGLLSEGYVLDDEVECPFHQGRFHIPTGSATCAPATEPLKTWKVRVVEGNVCI
                     DPAEHESAPV"
     gene            3555..>4672
                     /locus_tag="AC158Q_11215"
     CDS             3555..>4672
                     /locus_tag="AC158Q_11215"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HIR3340387.1"
                     /translation="MALTREQNDQLTQTGPGTLLGDLFRRYWVPAILAGEIAEPDGPP
                     VRVQLLGEKLVAFRDTEGRIGLISEFCAHRGVSLWFGRNEECGLRCPYHGWKYDVNGQ
                     CVDLPSEAESSGMRSRIKLKSYPCIEQGGVIWTYMGPPELKPAPPALEWTLVPGEQRF
                     VSKRLQECNYLQAIEGGIDSSHVSFLHSGALNRDPLFKEGKRANTYNLSDRMPHFDVV
                     EYEGGLLIGARRKADKDRYYWRVTPFIMPWHTLIPPRAGHPIGGHAWVPIDDENCWAW
                     SINYHPKRALTASEVKAMKDGKGIHVKYVPGTFIPLANRTNDYLMDRSTQRSGANYSG
                     VEGIAMQDASLQESMGPIQDRTRENLCSTDNGIIMMRRL"
BASE COUNT          750 a         1499 c         1565 g          858 t
ORIGIN      
        1 gtctgcggcg agacggcgtt catgtcggcg aaagcgatct tgcgccccgt cttcctcatc
       61 gcgtcggcca cgttgcgcgc gacggcgggt gattggccgg ggtcgatgac cgagatcacc
      121 agctcgcagg tggagacgag cttctcgatc gagccgcaat cggcgaggcc ggcttcgcgc
      181 gccagcgcct tcgtgcgctc gctgcgcccg tcgagcgcgg tgtagacgtt cattccggat
      241 tccttgagcc ggccggcaat ggcctggccc atgtcgccgg ggctgacgat gccgatattg
      301 cggatttgca tgcgcgtgcc tttcctgtgg ggtgatgtcg cggagcagtg cgatgtgcct
      361 gcgctagaat accgcttttc cgccgcgcct ggcggcaatt ctcgccgcca tcaggatcca
      421 tttgatccct taaatgaaca ttgccctcgc cgccggggcg gctggagcgg cgcttggggt
      481 gcgtacctta ttcttcgcta ttgcgtcccg gatgcgggcg gcggccatgc ccgccgtcgc
      541 ggggcgcgca aggagcgaga tgtcgttctg gctgatcgtg ctggtcgtgg tgctgaacca
      601 tggctgcctg aaaggcgcca aggtattgat gtcgctttcc tccatcgagc tgggcgcgag
      661 cccggcgacg atcggcttgc tctacgcgat gtactcgctg ttgccggcgc tgctgtccgt
      721 atatgccggc aagctgtccg accgccgcgg attcgggtct ctggcgaccc tcgcttccgc
      781 gggcctttgc gccgggctcc tgctgccgta ctgcttcgcc gggctgggga cgctgttcgc
      841 gtcggccgcg atcgcgggcg cgagctacat cttctatatc gtcgcgatcc agcacctgtt
      901 cggttccatc ggggagggcg ccgatcgcac gcgcaattac ggcatcttcg cgatctgcgt
      961 cagcgtgacg tcgctggtcg ggccggtgac tgccggattc agcatagacg tcatcggaca
     1021 tcgggggagc tggctgctga tcgccagctt tccggcggtg gcaacgctgg cgctttggct
     1081 gttctcggca cgcctgccgg tgcacaggcc gcacgagcgg ccggcgggcc ggcagcggct
     1141 gctcgatttg ctgcgcaacg cgccgctgcg ccgcgtgctg ctggcgactg cctttctcga
     1201 aaccggcatg gagctgttca atttcttgat gccgatctac ggacactcca tcggcctctc
     1261 cgcttcccgg atcggtatcg tcatgggttc gttcgcgctg gccatgatgc tggtgcgcat
     1321 cgtcatgccg cggctcgcca ggcgctggag tgaagaacgg gtgttcagcg tctcgctttt
     1381 cgtggcggga agcgtctcgc tggtattccc gctggtgtcg ggtttcgtgc cgctgctcgc
     1441 ggccgctttc gtgctcggat tgggattcgg ttccggcgcc cccttgtcga tggcgctctc
     1501 gtacaaccgg gcgcccgccg ggcgcacggg ggaagccatc gggttgcgcc agaccgtaaa
     1561 caagggaatc gaggcgacgg ttccggccgc attcggtttt ctcagcgccg cgttcggcat
     1621 ggttcccgtg tactgggtcg gggcactgct gctcggctgc ggcggctggt tgatgcacgg
     1681 cgatgcgcgc cgaaaatctg caaagcggaa ttcgtgattc gggtgttttg cggccagctg
     1741 caggcggcgc cgtaaccggg tatgagtctt gatctgcgtc aacaccgacg cctccgcccg
     1801 gtttagaatc ccgccagtat cgcgagggtc tcgagatgaa gctgcgcaaa ctgcccgtcg
     1861 gcgtgcaccc caacctgctt cacccggcct acaggagcac cgtcgagcgg gcgccccggc
     1921 aacgtcctat cgcgcttccg ccgggcgcaa ccgagcagag cggaccgcgt ttcgacccgg
     1981 cggtcctgta cccgcaccac gacgatctca cgcggcaggt ggaggggggc gaggctgcgg
     2041 gccagcgcat catcgtgacc ggcagggtcc tggatgagaa cggcgacccg ctgccggaca
     2101 cgctggtgga aatctggcaa tgcaacgcgg ccggccgcta ccatcatggc caggacaacc
     2161 acgacgcgcc gctcgatccg tacttccgcg gcgggggcag gatgctgacc gacgccgccg
     2221 gcgcctatcg tttcaccagc atccggcccg gcgcctaccc ctggcgcaat cacccgaacg
     2281 cctggcgccc ggcgcacatc cacttctcgg tgttcgggcg cacgatgctc gagcgcctga
     2341 tcacccagat gtatttcccc ggcgacccgt tgctgggcta cgacccgatc ttcaacagcg
     2401 tgccgaccga gcgggcgcgc gagcgcctga tctcccattt tgacctggaa gtcacgcagc
     2461 ccgagtgggc gctcggattt catttcgata tcgtgctgcg cgggccggag gccacgcccg
     2521 ccaacgacga gtgagccggg ggcaggcatg gccgagttat tgattacgcc ctcgcagacg
     2581 atcggtcctt tcttcaagta cggcctggaa tggccgggcg gagaatccct gtttccggat
     2641 tcggcgcagg gccgtcgcat ccggctgggc ggtgtcgtga ccgatttcaa ggacgaaccg
     2701 gtgcccgatg cgttaatcga gttctggcag gccgacgcca aggggcagtt cggtgccgcg
     2761 cgggccaagg caagcggtgg cttcggccgg gtggcaaccg acgagcgtgg ccgctatgcg
     2821 atcaatacca ttttcccggg accggtcgcc ggcgccaacg ggcgcaagca ggcgccgcac
     2881 atactcgtgg tcctgttcgc gcgcggcctg ctcgcccagg tcgtcacccg ggtctacttc
     2941 gaaggagagg ccgccaacaa ggatgatccg gtcctcgcct tgtgcggcgt gcgcgcgtcc
     3001 acgctcatgg ctaaacgggt cgctgacgat gcgaatgcct atgattggga catttccctc
     3061 cagggccaga acgaaaccgt gttcttcgag gcgtaatcgg gggaatgacc gcgcgggaat
     3121 ttctgttccg cggggaagaa cggattttcg cctggcgcca ttgcgcgtcg cgtcgggtaa
     3181 aatatccggc atgaaagacg atcgctggat cccgctttgc ggcgtggcgg atgtcgcgcc
     3241 gggatgcggg cttcgcgcgg aaacgggagg ctgcgtgtac gccgtattca acgtcgaggg
     3301 caggttctac gtgacccagg acgaatgcac gcacgggccc ggcctgctgt ccgagggcta
     3361 cgtgctcgac gacgaggtcg agtgcccgtt ccaccagggg cggtttcaca tcccgaccgg
     3421 cagcgcgacc tgcgcgcccg ccaccgaacc gctgaaaacc tggaaagtgc gggtcgtcga
     3481 aggcaacgtc tgcatcgatc cggcggaaca cgaaagcgcg ccggtctgaa cgggatgaag
     3541 cgacggagga cgccatggca ctcacgcggg aacagaacga tcaactgact cagaccggcc
     3601 ccggcacgct gctcggggat ctcttcaggc gctactgggt acccgcgatc ctggccgggg
     3661 agatcgccga gcccgacggc cctcccgtgc gcgtacagtt attgggcgag aagctcgtcg
     3721 ctttccggga caccgagggc cgtatcggcc tgatcagcga attctgcgcg catcgcggcg
     3781 tctcgttatg gttcggccgc aacgaagagt gcgggctgcg ctgtccctac cacggctgga
     3841 aatacgacgt gaacgggcag tgcgtggatc ttccgtccga ggccgaatcc tcgggcatgc
     3901 gttcccgcat caagctcaag tcctatccct gcatcgagca gggcggggtc atctggactt
     3961 acatggggcc ccccgaactg aagccggcgc ctcccgcgct ggaatggacg ctggtgcccg
     4021 gggagcaacg cttcgtgtcc aaacgcctgc aggagtgcaa ctacctgcag gccatcgagg
     4081 gcggcatcga ctcgagccac gtgtcgttcc tgcacagcgg tgcgcttaac cgcgacccgc
     4141 tgttcaagga gggcaaacgg gccaacacgt ataacctgag cgaccgcatg ccgcatttcg
     4201 atgtggtcga gtacgagggg gggcttctca tcggcgcgcg ccgcaaggcg gacaaggacc
     4261 gctattactg gcgcgtgaca cccttcatta tgccgtggca tacgctgatc ccgccgcgag
     4321 ccggccaccc catcggcgga catgcgtggg tgccgatcga cgacgagaac tgctgggcct
     4381 ggtccatcaa ctaccatccg aagcgcgcgc tgacggcatc cgaagtgaag gcgatgaagg
     4441 acggcaaggg catccacgtg aagtacgtgc ccggcacctt catcccgctc gccaaccgga
     4501 cgaatgatta cctgatggac cgttcgacgc agagatcggg cgcaaactac agcggcgtcg
     4561 agggcatcgc gatgcaggat gcgtccctgc aggagagcat ggggccgatc caggaccgca
     4621 cccgggagaa cctctgctcg acggacaacg gcatcatcat gatgcgccgc ct
//
LOCUS       DBNGSG010000430         3252 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2516416, whole genome shotgun sequence.
ACCESSION   DBNGSG010000430 DBNGSG010000000
VERSION     DBNGSG010000430.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2516416"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3141)
                     /locus_tag="AC158Q_11220"
     CDS             complement(<1..3141)
                     /locus_tag="AC158Q_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HIR3340388.1"
                     /translation="MDFSRGIAPHCRTLAVVPALLTHAQGVETLVESLEVRFLANRDP
                     NLYFGLLTDYEDAPEETQPADGPLLELARARIEELNRQYRHADDRSGGDPFFLFHRPR
                     RWNAQERVWMGYERKRGKLADLNALLRGDGEGRFALVLGTTHVLSTVKYVITLDSDTQ
                     LPRDSARQFVGTMAHPLNQPRFDPAAGADGQRVIEGYAILQPRVAVSLPGTNRSRFAR
                     LFGSEPGIDPYTRAVSDVYQDAFGEGSFIGKGIYDVDAFERSLKDRFPENRILSHDLL
                     EGCYARAALLSDVQLYEEYPASYSADVARRHRWIRGDWQIASWLLSRVPEAGAARQGN
                     PLSALSQWKIFDNLRRSLVPAGLTLLLMLGWTLSSQPWLWTLAVIGIIVIPSLSASIL
                     ELLRKPDEVLPVQHIAATLQSAGRHLAQAALELACLPHEAWFSLDAIVRTNWRILATR
                     RKLLEWNPSSDSARACERFGRASLPGSYLSMWTAPFIGIAAAVLLVVTHPPALAVAGP
                     ILFLWLVAPAITWWISRPLARRVEKLTGEQTLFLRKLARRTWAFFETFVGPEDHGLPP
                     DNYQEHPVPVVAHRTSPTNMGLGLLANLSAYDFGYLPAGQLIERTANALHTMAALERH
                     QGHFYNWYDTRSLKPLLPLYISSVDSGNLAGHLLTLRPGLLALPDHRILGPRLFEGLS
                     DTLGVLMDAAGQSASAQLGPFRKDLETAYDSRPATIAAAHRWLTRLSDAAAQVARSFE
                     SGGGIAAPAAVASEIDAGWWAQALARQCRGALVELDALAPWLALPAAPGRLQASVDGA
                     VIPTLRELAQFETTWLPAVEQRAGPGTTHGEREWLSGLRRHITTAGGRARERIAAIEA
                     LALRAGELAQMEYGFLHNEARHLLAIGYNVAEHRQDPSCYDLLASEARLTTFVAIAQG
                     QLPQESWFSLGRLLTNTGGEPALLSWSGSMFEYLMPLLIMPTHENTLLDRTYASAVAR
                     QIAYGKQRGIAWGMSECGYNAVDAAFNYQYRAFGVPGLGLKRGLTEDVVVAPYASALA
                     LMVAPEAACTNLQRLAA"
BASE COUNT          560 a         1083 c         1100 g          509 t
ORIGIN      
        1 ggcggcaagc cgctgcaggt tcgtgcatgc cgcctccggc gcgaccatca gcgcgagcgc
       61 cgaagcgtac ggcgccacca ccacgtcttc cgtcagccct cgtttgagcc cgaggccggg
      121 cacgccgaac gcgcgatatt gataattgaa agcggcgtcg accgcgttgt agccgcattc
      181 cgacatgccc catgcgatgc cgcgctgttt cccgtaggcg atctgtcttg ccactgccga
      241 tgcataggtc cggtcgagca gcgtgttctc gtgcgtcggc atgatcagga gcggcatcag
      301 gtactcgaac atcgagccgc tccacgacag caatgccggc tcgccgccgg tattggtgag
      361 caggcgccca aggctgaacc agctttcctg cggcaattgt ccctgcgcga tcgcgacgaa
      421 agtggtcaac cgcgcttccg aggcgagcag gtcatagcag ctcgggtcct gccggtgctc
      481 ggcgacgttg tagccgatcg cgagcaggtg gcgcgcctcg ttgtggagga agccatactc
      541 catctgcgcg agttctcccg cccgcagcgc cagggcttcg attgctgcaa ttcgctcccg
      601 ggcgcggccg cctgccgtcg tgatgtgccg ccgcagcccg gacagccact cgcgctcccc
      661 gtgcgttgtg ccgggccccg cgcgctgctc gacggccggc agccacgtcg tttcgaattg
      721 ggccagctcg cgcagcgtcg ggatgaccgc gccgtccacc gatgcctgga gccggccggg
      781 cgcggcgggc agcgcgagcc atggggccag cgcgtcgagc tcaacgagag caccgcggca
      841 ctgccgcgcc agcgcctgtg cccaccatcc cgcatcaatc tcggacgcaa cggcggcggg
      901 cgcggcaatg ccgccgccgc tctcgaagct gcgggcgact tgcgcagcgg catccgatag
      961 ccgcgtcagc catctgtgcg cagcggcgat cgtggcgggc cgggaatcgt aggccgtttc
     1021 caggtccttc ctgaacgggc cgagctgggc ggaagcggac tggcccgcgg cgtccatgag
     1081 gactcccagg gtgtcgctca atccctcgaa caatcgcggt cccaggatcc ggtgatcggg
     1141 aagggcgagc agtcccggcc gcagcgtcaa aaggtgtccc gccaggttcc cgctgtctac
     1201 cgacgaaatg tagagaggca gcagcggctt caacgaccgt gtgtcgtacc agttgtagaa
     1261 gtgaccctgg tgccgttcca gggcagccat cgtgtggagc gcattcgccg tgcgctcgat
     1321 gagttgtccg gccggcaggt agccgaagtc gtaggcggac agattcgcga gcagcccaag
     1381 acccatgttg gtaggcgagg tgcgatgcgc gacgaccggg accgggtgct cctggtagtt
     1441 gtccggcggc agcccgtgat cttccggccc gacgaaggtc tcgaagaacg cccaggtccg
     1501 gcgcgcgagt ttgcgcagaa agagagtttg ctcgccggtc agtttctcga cgcggcgcgc
     1561 aagcggccgg ctgatccacc acgtgatggc gggcgcgacg agccacagga acagtatcgg
     1621 cccggcgacc gccagcgcgg gcggatgggt caccaccagc aaaaccgctg ccgcaatgcc
     1681 gatgaagggc gccgtccaca tggacagata agagccgggg aggctcgcgc ggccgaatcg
     1741 ttcgcaggct cgcgcagaat cgctcgacgg attccattcg agcagttttc tgcgggttgc
     1801 caggattcgc cagttggtgc ggacgattgc gtcgaggctg aaccacgctt catggggaag
     1861 gcaggccagc tccagtgctg cctgcgccag gtgccgcccc gccgattgca gggtggcggc
     1921 gatatgctgg accgggagca cttcgtcggg cttgcgcaac agctccagga tcgacgcgct
     1981 cagggaggga atcacgataa tgccgatcac cgccagggtc cataaccagg gctgcgacga
     2041 cagcgtccag cccagcatca acaggagcgt cagcccggcg gggaccaggc tgcgccgcag
     2101 gttgtcgaaa atcttccatt gggacagggc cgacagcggg ttcccctggc gggcggcgcc
     2161 ggcctcgggc acgcgcgaca gcagccacga ggcaatctgc cagtccccgc gtatccagcg
     2221 gtggcggcgg gcgacgtccg cgctgtaact ggccgggtat tcctcataca gctgcacatc
     2281 gctcaacagc gccgcgcgcg cgtaacagcc ttccagcaga tcgtggctga gtatgcggtt
     2341 ctcgggaaag cgatccttga gcgatcgctc gaacgcatcg acgtcgtaga tgcctttgcc
     2401 gataaacgag ccctcgccga acgcgtcctg gtaaacatcg gagacggcgc gcgtataagg
     2461 gtcgatgccg ggctcgcttc cgaacaatcg cgcgaagcgc gaccggttcg tgcccggcag
     2521 gctcacggcg acgcgcggtt ggaggatggc gtagccctcg atcacgcgct gaccgtccgc
     2581 gcccgccgcc ggatcgaagc gcggctgatt caaggggtgc gccatcgtgc ccacgaactg
     2641 ccgcgccgaa tcgcgcggca attgcgtgtc cgagtccagc gtgatgacgt acttgaccgt
     2701 agacagcacg tgggtcgtgc ccagcaccag cgcgaaccgg ccctccccgt cgccgcgcag
     2761 cagggcattc aggtcggcga gcttgccgcg cttgcgctcg tagcccatcc atacccgctc
     2821 ctgcgcgttc cagcggcgcg ggcgatgaaa aaggaagaac gggtcgccgc cggaacggtc
     2881 atccgcatgc cggtactgcc gattcagctc ttcaatgcgt gcccgggcca attccagcaa
     2941 cggtccgtcc gccggctgcg tttcctccgg cgcgtcctca tagtcggtca gcagcccgaa
     3001 gtagaggttg ggatcccggt tggcgaggaa gcggacttcg agcgactcga ccagggtctc
     3061 cacgccttgc gcgtgcgtga gcagggcagg gaccaccgcc agcgtgcggc agtgcggcgc
     3121 aatccccctg gaaaaatcca tgcgcggcag aggccgcggc gtcaccagca atgtcgcgag
     3181 ccaattcacc agcgcgaccg ccagctggct cgtgcagagc gcgatgacga taccgctcga
     3241 ggcaagcagc ca
//
LOCUS       DBNGSG010000431         7396 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_479689, whole genome shotgun sequence.
ACCESSION   DBNGSG010000431 DBNGSG010000000
VERSION     DBNGSG010000431.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7396)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7396)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7396
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_479689"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..902
                     /locus_tag="AC158Q_11225"
     CDS             87..902
                     /locus_tag="AC158Q_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007030 - Golgi organization [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HIR3340389.1"
                     /translation="MCLILLAWHAHPDYPLVFAGNRDEAYERPSAAADFWNDDPAIYG
                     GRDLEKGGTWLGLTRTGRLAAVTNYRDGPARKPAPRSRGELTANFLRSDDGPEAYLKN
                     IAPNVTQYGGFSLLVGDSERLWSCSKCGAGIEEVAPGVHGLSNHLLDTPWPKVTRGRQ
                     KVAALLEAGETELIKGLFDALADRSVAADAELPDTGVGAGRERELSPAFIAGERYGTR
                     ASTVLFVSHANEVTFIERRFGALGAPQGETTRQFALQDIRARGRVTKAAGQDV"
     gene            complement(848..1339)
                     /locus_tag="AC158Q_11230"
     CDS             complement(848..1339)
                     /locus_tag="AC158Q_11230"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3340390.1"
                     /translation="MSDKPLFTVKPVYWNASEKTLRAIRTTVFVEEQKVPVDLEWDGL
                     DEHAYHVIAFAGDGTPIGTGRLLQDGHIGRLAVLTEWRGKGVGRALLDMLLVIANKMG
                     YDNVKLHAQTRVLDFYLRRGFEPQGEEFMEAGIPHVLMTRTTADQTSWPAAFVTRPLA
                     RMS"
     gene            complement(1492..2316)
                     /locus_tag="AC158Q_11235"
     CDS             complement(1492..2316)
                     /locus_tag="AC158Q_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP reductase"
                     /protein_id="HIR3340391.1"
                     /translation="MADKPRNGNGHGKEIGGSNGKNGNGHASARLAGKVAIITGAGRG
                     IGHATSLKFGHEGAIVIACDINADQAQQTAQDVIDGGGEALSCQIDVRDPASIARMVD
                     EVVRKYGRIDCLVNNAGIVQDATLKNMSEAQFDDVIEINLKGVYNCTRAVVDTMLKQQ
                     SGVILNASSIVGLYGNFGQTNYAASKFGVIGMVKTWAKELGRKGIRANAVCPGFVATP
                     ILSKIPEKVLKALEERVPLGRLARPEEIANTFAFLASDEASYINGAVIEVSGGLTL"
     gene            complement(2485..2967)
                     /locus_tag="AC158Q_11240"
     CDS             complement(2485..2967)
                     /locus_tag="AC158Q_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR3340392.1"
                     /translation="MSRIRINIKSQELDLYDDSGALLKRYRISSAKNGVGEQNGSYCT
                     PRGKHIVRAKIGAGHPVNTVFVERRPTGEVYSPELAKGFPRRDWILTRILWLSGCEPG
                     RNRLGRVDTMRRAIYIHGSPDRAEMGKPGSHGCIRMRNQDIVELFDLVPVRTPVEILE
                     "
     gene            complement(2960..3424)
                     /gene="tadA"
                     /locus_tag="AC158Q_11245"
     CDS             complement(2960..3424)
                     /gene="tadA"
                     /locus_tag="AC158Q_11245"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HIR3340393.1"
                     /translation="MSDETYMREALALAREAAAAGEVPVGAVVVKEGTVIGRGYNRPV
                     STRDPTAHAEVIALRDASGRLGNYRLAGCALYVTLEPCAMCAGAIMHARIERLVYGAA
                     DPKSGACGSVIDLFSEGRLNHHAGVASGVLAGEAGKLLQDFFSVRRQAPGHV"
     gene            complement(3441..3797)
                     /locus_tag="AC158Q_11250"
     CDS             complement(3441..3797)
                     /locus_tag="AC158Q_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HIR3340394.1"
                     /translation="MGITIYGIRNCDTVKKARAWLEAHEIEHDFHDYKVAGIDRNRLE
                     RWCGEFGWELLLNRAGTTFRKLPDADKRSLDARKAIALMVAQPSLIKRPVLDLGGRRL
                     VGFKPELYESAVRALR"
     gene            complement(3802..5385)
                     /gene="guaA"
                     /locus_tag="AC158Q_11255"
     CDS             complement(3802..5385)
                     /gene="guaA"
                     /locus_tag="AC158Q_11255"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIR3340395.1"
                     /translation="MQHEKILILDFGAQYTQLIARRVREARVYCEIHPHDVDDAFVRT
                     FNPRGVILSGGPASVWEGGTPRAPQSVFELGVPVLGICYGMQTMADQLGGKVEMGKVR
                     EFGYAELRARGHTALFKGIQDRVTPEGHGLLDVWMSHGDKVVEMPQGFKLMASTDTCT
                     IAGMADEKRRFYAVQFHPEVTHTPQGKAILTRFVREICGLSGDWSMPDFMDEEIARIR
                     LQVGAEEVVLGLSGGVDSSVAAALIHKAIGQQLTCVFVDHGLLRLNEAEQVMYTFAGN
                     LGVKVLHVDARDQFMGHLKGVADPEQKRKIIGREFIEVFQSEASKLPRVKWLAQGTIY
                     PDVIESAGSRTKKAHNIKSHHNVGGLPDTLHLKLLEPLRELFKDEVRELGLALGLPRE
                     MVFRHPFPGPGLGVRILGEVKAEYADLLRRADAIFIDELRATKDADGVGWYDKTAQAF
                     AVFLPVKSVGVMGDGRTYEYVVALRAVETTDFMTARWAHLPHELLAKVSNRIINEVRG
                     INRVAYDISSKPPATIEWE"
     gene            complement(5479..6942)
                     /gene="guaB"
                     /locus_tag="AC158Q_11260"
                     /pseudo
     CDS             complement(5479..6942)
                     /gene="guaB"
                     /locus_tag="AC158Q_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065652.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
     gene            7069..>7396
                     /locus_tag="AC158Q_11265"
     CDS             7069..>7396
                     /locus_tag="AC158Q_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340396.1"
                     /translation="MKAAIALVLMLAVVPAHGQLLKCISKDGKVEYATQCPAGTKEQA
                     TGIKSAPGTSSPGTASKPKTMAEKEADFRKRQAEQAEAQGKEQKESAEAAQRQRACEE
                     ARNYLAG"
BASE COUNT         1338 a         2380 c         2214 g         1464 t
ORIGIN      
        1 aacggcctga atatttgccc gaaatttacg ggacgccact ctagattgtt ctttcctcat
       61 ccctcatccc ttcgccctca tccctgatgt gtctcatcct tctcgcctgg cacgcccacc
      121 ccgactaccc gctggtcttc gccggtaacc gggacgaggc ctatgagcgt ccgtccgccg
      181 cggcagattt ctggaacgat gatccggcca tctacggcgg ccgcgatctc gagaagggcg
      241 gtacctggct tggactgacg cgtaccggcc gactggcggc ggtgaccaac tatcgcgatg
      301 gtcccgcgcg caagccggcg ccacgctcgc gcggagagtt gacggcgaac ttcctgcgca
      361 gtgacgatgg gcctgaggct tacctgaaaa atatcgcccc caacgtcact caatacggcg
      421 gcttttccct gctggtcggc gatagcgaac ggctgtggag ctgttccaaa tgcggcgccg
      481 ggatagagga agtggccccc ggcgtgcacg ggttgagcaa ccacctgctc gatacgccct
      541 ggccgaaggt cacgcgcggc aggcagaaag ttgctgccct gctcgaggcg ggcgaaacgg
      601 agttaatcaa agggctgttc gacgcccttg ccgaccgctc cgtcgctgcc gatgcggaac
      661 tgcccgatac gggggtggga gccggtcgtg agcgggagct gtctcccgct ttcatcgccg
      721 gcgaacgcta cggcacgcgc gcttccaccg tgctgtttgt cagccacgcg aatgaagtga
      781 ctttcatcga gcgccggttt ggcgccctgg gcgcgccgca gggggaaacc acgcgacagt
      841 tcgcacttca ggacatccgc gccagggggc gcgtcacgaa agcagccggc caggacgtct
      901 gatcggccgt cgtgcgggtc atcaggacat ggggaatgcc cgcctccatg aattcctcgc
      961 cctgcggctc gaacccgcgc cggaggtaga aatcgaggac gcgggtctgg gcatgcagct
     1021 tcacgttgtc ataacccatc ttgttggcga tgacgagcag catgtcgagc agggcccggc
     1081 ccacgccctt gccgcgccat tctgtcagca ccgccaggcg accgatatgc ccgtcctgca
     1141 gcagccggcc ggtgccgatc ggcgtgccgt cccccgcaaa cgcgatgacg tgataggcgt
     1201 gctcgtcgag gccatcccat tccaagtcca ccggtacctt ctgctcttcc acgaacaccg
     1261 tggtgcgaat cgcccgcagc gttttttcgc tggcgttcca gtaaaccggc ttgaccgtga
     1321 acaggggctt gtcactcatg ggggccatta taagtaaccc caaggttaac agcagtaatg
     1381 cccggccgcg atggccgctc ggcccggacc gaaaccagat attcgtggaa gaataggtga
     1441 aatggcggcc cggaaccctc cgataggccg cggacggctt gaaccagctc gctacagggt
     1501 cagcccgccg ctgacttcga tcaccgcgcc gttgatgtag ctcgcctcat ccgaggcgag
     1561 aaaggcgaag gtgttggcga tttcttccgg ccgggcgaga cggccgagcg gcacccgttc
     1621 ctcgagcgcc ttgagcactt tctcgggtat cttgctgaga atcggggtgg cgacaaaccc
     1681 cgggcagacc gcgttggcgc ggatgccctt gcggcccagt tccttggccc aggtcttgac
     1741 catgccaatg acgccgaact tgctggctgc atagttggtc tggccgaaat tgccgtaaag
     1801 cccgacgatg gaagatgcgt tgaggatgac gccggattgc tgcttgagca tggtatccac
     1861 caccgcgcgg gtgcagttgt agacgccttt caggttgatc tcgatcacgt cgtcgaattg
     1921 ggcctcgctc atgttcttga gcgtcgcgtc ctggacgatg ccggcattgt tcaccaggca
     1981 atcgatccgc ccgtatttcc tgacgacctc gtccaccatg cgcgcgatac tcgccgggtc
     2041 ccgaacgtcg atctggcagc tcagcgcctc gcctccgccg tcgatgacgt cctgggccgt
     2101 ctgctgggcc tggtcggcgt tgatatcgca ggcgatgacg atggcccctt catggccgaa
     2161 tttcagcgag gtggcgtgcc cgatgcccct gccggccccg gtgatgatgg cgaccttccc
     2221 ggccagcctt gcgctggcgt gaccgttacc gttcttgccg ttgctaccgc cgatttcctt
     2281 gccgtgcccg ttgccgttac gcggtttatc agccatggtc ccggtaaacc ccaagctatg
     2341 agatgcggca atttaactca ttttctcgca ttgcacaata atcgcactac cccaaggaat
     2401 agtggctcct aacccattgg atacacaacc ggttgcggga gatgttctta tttggcaatg
     2461 caagatcaag cgcttgcagc aggcctactc aagaatctct acgggggtgc ggaccggcac
     2521 gagatcgaac aactcgacaa tatcctgatt acgcatgcgg atgcagccgt gcgagccggg
     2581 cttgcccatc tcggccctat ccgggctgcc atggatgtaa attgcgcggc gcatggtatc
     2641 gaccctgccg agccgattgc gccccggctc gcagcccgac agccagagga tgcgggtcag
     2701 gatccagtcg cgcctgggaa atcccttagc cagctccggg ctgtaaacct cgccggtcgg
     2761 gcgccgctcc acgaacacgg tgttcaccgg atgcccggcg ccaatcttgg cgcggacaat
     2821 gtgcttgccg cgcggggtgc aatagctgcc gttttgctcg cccaccccgt tctttgcgct
     2881 cgagattcga tagcgcttga gaagagcgcc gctatcatcg tatagatcaa gctcttgtga
     2941 cttgatatta attcttattc tagacatggc ctggtgcctg tcgcctcacc gagaagaagt
     3001 cctgcagcag cttgccggct tccccggcca acacgccact ggcgacccct gcatggtgat
     3061 tgagccggcc ctcggagaac aagtcgatga cgctgccgca agcgccggac ttgggatctg
     3121 ccgctccata cacgagccgc tcgatgcggg cgtgcatgat cgcgcccgcg cacatcgcgc
     3181 atggctcaag cgtgacgtaa agagcgcagc cggcgagacg gtagttgcca agacgcccgg
     3241 acgcgtcccg caacgcaatt acctccgcat gtgccgtggg atcgcgcgtc gaaaccggtc
     3301 tgttgtagcc gcgcccgatg accgttccct ccttgaccac cacggcgccg acgggaacct
     3361 cacccgccgc agccgcctcg cgggcgagtg caagcgcttc ccgcatgtag gtttcgtcgc
     3421 tcatattcgt aacccccagg ctaccggagc gcgcgcactg cactttcgta cagctccggt
     3481 ttgaacccca ccagccggcg tccgcccagg tccagcaccg gccgcttgat caacgaaggc
     3541 tgcgccacca tcagggcgat ggctttgcgg gcatcaagtg atctcttgtc ggcgtccggc
     3601 agcttgcgaa acgtggtgcc cgcacggttc aaaagtaact cccagccgaa ctcgccgcac
     3661 cagcgctcca ggcgattgcg gtcgattcct gcaactttgt aatcgtgaaa gtcgtgctcg
     3721 atctcatgag cctccagcca ggcgcgggcc ttcttgaccg tgtcgcaatt acggatgccg
     3781 tagatcgtga tgcccactct cctactccca ctcaatcgtc gcgggtggct tgctcgagat
     3841 atcgtaggcg acgcgattga tcccgcgcac ttcattgatg attcgattgg acaccttcgc
     3901 cagcagctcg tgcggcaggt gcgcccagcg ggcggtcatg aaatcggtgg tttcaacagc
     3961 gcgcagagcc accacatatt cgtaggtgcg gccgtcgccc atcaccccta cagacttcac
     4021 cggcagaaac acggcgaacg cctgcgcggt cttgtcatac cagcccacgc catcggcgtc
     4081 ttttgtggcg cgcagttcgt ctatgaagat tgcatcggcc cgccgcagca ggtccgcgta
     4141 ttccgccttc acctctccga gtatgcgcac gcccagcccc ggccccggaa atgggtggcg
     4201 gaacaccatt tcgcgcggca gccccagcgc aagacccagc tcgcgcacct cgtccttgaa
     4261 gagctcacgc agcggctcga gcagcttgag gtgcagcgtg tccggcaggc cgccgacgtt
     4321 gtggtgcgac ttgatgttgt gcgccttctt ggtcctggag ccggccgatt cgatcacgtc
     4381 gggataaatg gtgccttgcg ccagccattt gacccgcggc agtttcgacg cctcactctg
     4441 gaatacctcg atgaattcgc gaccgatgat cttgcgcttc tgctcgggat cggccactcc
     4501 tttgaggtgc cccatgaact gatcgcgcgc gtccacgtgg agcactttga cgccgagatt
     4561 gccggcgaaa gtgtacatca cctgctcggc ctcgttgagc ctcaacaggc cgtggtccac
     4621 gaaaacgcag gtgagctgct ggccgatcgc cttgtggatg agcgccgcgg caacggacga
     4681 gtccacgccg cccgaaaggc ccagcacgac ttcctccgcg ccgacttgta accggatgcg
     4741 cgctatctcc tcatccatga aatccggcat gctccagtcg ccggacaggc cgcagatctc
     4801 ccgcacgaat cgagtgagga tggccttgcc ctgaggcgtg tgcgtaacct cgggatggaa
     4861 ttgcacggca tagaacctgc gcttctcgtc ggccatgccc gcgatcgtgc aggtgtcggt
     4921 ggaagccatc aacttgaagc cctgcggcat ctcgaccacc ttgtcgccgt ggctcatcca
     4981 cacgtcgagc aggccgtggc cttcgggggt tacccggtcc tggatgccct tgaatagggc
     5041 cgtgtggccg cgcgcgcgca actcggcgta gccgaattcg cgcaccttgc ccatctcgac
     5101 cttgccgccc agttgatccg ccatggtctg catgccatag cagatgccaa gcaccggcac
     5161 gcccagctcg aacacggatt ggggggcccg cggcgtgccg ccttcccaga cagaagccgg
     5221 tccgcccgag aggatgacgc cgcgcggatt gaaagtgcgg acgaacgcgt cgtccacgtc
     5281 gtggggatgg atttcgcagt aaacgcgcgc ttcgcgcacc cgccgcgcga tcaactgcgt
     5341 gtactgcgcg ccgaaatcga ggatgaggat tttctcgtgc tgcatttacc cgtgaaacgt
     5401 gaaccgtgaa acgtgaatcg tgcaccagcg gctatcagcg gcccgtcgtt tcacgattta
     5461 cgattcacca ttcacgattt actcgactct gtaattgggc gcttccttgg tgatctgcac
     5521 gtcgtggacg tgggactcgc gcatgcctgc ggagctgatt tccacgaatt cagccctggc
     5581 acggacctcg tccgtggtat tgcagcccag atagcccatg ctcgatcgca agccgcccac
     5641 gagctgctgc accagcggcg cgagctgccc tttgtacggc acccttccct cgacgccctc
     5701 cggaaccagc ttgtcggtgt ccagctcgtc atcctggaaa tagcggtcgg cagcgccctg
     5761 ctgcatggcc gacagcgagc ccatgccccg gtaagacttg taggaacggc cctgaaacag
     5821 ctcgatctct cccggcgatt cttcggtccc ggcgaagaga ctgccgatca taaccgcgtg
     5881 ggcgccggcg gcgattgcct tcgcgatatc gccggaatag cgtatcccgc cgtcggcgat
     5941 cagcggcacg tcggtcttgc tgagtccttt ggcgacattg tcgatcgcgg taatctgcgg
     6001 cacgccgatc ccggcgacaa tgcgcgtggt gcagatggag cccggcccga tgccgacttt
     6061 cactccgtcc gcgccatgat ccacgagcgc cctggctgcc gccgcggtgg cgatattgcc
     6121 gccgatgacc tgggttttgg gaaaattccg cttcacccat ttcacgcggg cgagcacgtt
     6181 tttcgaatgg ccatgagctg tgtccactac gatgacatcg gcgcctgcct cgaccagcgc
     6241 ctccgcccgt tcctccgtgc cctcgcccac gccgatcgct gcgccaacgc gcaggcggcc
     6301 caggttatcc ttgcatgcat tgggatgttc ggtggatttg aggatgtcct tgaccgtgat
     6361 caggccgcgc agctcgaatt tcttgttgac caccagcacc cgctcgaggc ggtgcttgtg
     6421 catgagcctc atcgcctctt cacggctcgc gccctcgttt acgatcacca ggcgctcgcg
     6481 cggcgtcatg atgttcttga ccggctggtc gagttttttc tcgaagcgca ggtcgcgatt
     6541 ggtaacgatg cccacgacgc gccccgaacc gttgatcacg ggtaacccgg atatgcggtg
     6601 ctgctgggtg atcttgagca cctcccgcac cttcatgtcg ggcgtgacgg tgatcgggtc
     6661 tttgacgatt ccgctctcga agcgcttgac cttggtcact tccgcggcct gagatttggg
     6721 cggcatgttc ttgtggacga tgcctattac cccttactgg gctatggcaa tcgcgagccg
     6781 ggcctcggtc acggtgtcca tggcggccga agccaggggg atattgaggg taatgccgcg
     6841 agtcagctgg gtgatcaggc tgacttcgcg aggaagtacg gtggagtggg cgggaatcag
     6901 caggacatcg tcgaaggtga gggctttttg caccacacgc atgttttcta cccttcgcaa
     6961 aaaccgtatt atacgcatgc accggtcaac ggtaaacgga ttgaagcgtt aatggattga
     7021 ctggattgat gaaagttgcg ccaaacgtgt caaatgagga gtgatcccgt gaaagctgcc
     7081 attgcgctcg tgctgatgct cgcggttgtc cctgcccacg gccagttgct gaaatgcatt
     7141 agcaaggacg gcaaagtcga atacgcgacc cagtgtccgg cgggcaccaa ggaacaggcg
     7201 accggcatca agagtgcgcc gggcacttca tcaccaggca ccgcgtccaa gcccaaaacc
     7261 atggccgaga aggaagccga tttcaggaaa cggcaagcag agcaggcaga ggctcagggc
     7321 aaggagcaga aagagtctgc cgaagccgcc cagcgccaac gtgcctgcga agaagcgcgc
     7381 aattatcttg ccgggc
//
LOCUS       DBNGSG010000432         2773 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4555530, whole genome shotgun sequence.
ACCESSION   DBNGSG010000432 DBNGSG010000000
VERSION     DBNGSG010000432.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4555530"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..1617
                     /locus_tag="AC158Q_11270"
     CDS             103..1617
                     /locus_tag="AC158Q_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HIR3340397.1"
                     /translation="MHILLRLLPLLLLPALGFAQPVVNVGSKRFTESYILAEIVSQVV
                     NRSGEARAVHQPGLGNTAILFAALKSGAIHVYPDYTGTIAFELLGMRNVPDLARLNRE
                     LGRHGLAAGVPLGFSNNYALAMLESRAEALGIRRISDLHHHPRLTLALSQEFLNRKDG
                     WQALRLAYRLPFADARGLDHGLAYEAIAAGSLDVMDVYSTDPKIDRYRLRVLHDDRGF
                     FPAYDAVLLYRQDLPEKFPQSWRALQGLQEKITAGRMMEMNAAAELSAVPFSRIAEAF
                     LDGRSPAGNTGGPPERRSLMQALLGPDFWRLTGEHLVLVFASLALGVAAGVPLGVCAN
                     RMQGARAWILGAAGVLQTVPSLALLAFLIAALDRIGTVPAIIALFLYSLLPIVRNTET
                     GLAGVPRSMKDSATALGLRAGARLRYIELPLAARTMLAGIKTSAVINVGTATIAAFIG
                     AGGYGERIVAGLAVNDHALLLAGAIPAAAMALLIQGLFDILDRHQLPGGSRPPA"
     gene            1738..2571
                     /locus_tag="AC158Q_11275"
     CDS             1738..2571
                     /locus_tag="AC158Q_11275"
                     /inference="COORDINATES: protein motif:HMM:NF017081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HIR3340398.1"
                     /translation="MALIYLVAAKASLVFAIAPGYATAVWPPSGIAVASCLLLGERIW
                     PAVWLGATLANLPVANSLFAAPAIACGNTLEAVTCAWFVRQLIGIGPQFGRGEDVLKF
                     VAIAMAGCVIAATVAVHVLIADGAIGWQQFAANWWTWWQGDVTGIIIVTPLLLSWALH
                     KDAFPPPGRAGEFLILAFSTAVTVAAVFGDWFETGKAQTLTFIVLPFIIWGAFRFTQR
                     EVTTVAAIISVITIEYTLGGRGAFVAGTVNESLLLLLAFVSTVVVTGLVLTATLAQRA
                     R"
BASE COUNT          411 a          887 c          916 g          559 t
ORIGIN      
        1 acggatcgag tgaaaagtcg cgtggcggca tggtcggcga tgcagggggt ctccgcccta
       61 ttttgccagt gccgccgggg ttcagccata ctgcgagcag tgatgcatat tctactgcgc
      121 ctgttgccct tgctcctgct gcccgcgctg ggattcgccc agccggtcgt caacgtgggc
      181 tccaagcgct ttaccgagtc ctacatcctc gccgagatcg tgagtcaggt ggtaaaccgg
      241 tccggcgagg cccgggcggt gcaccagccg ggactgggca ataccgccat cctgttcgcc
      301 gcgctgaaga gcggcgcgat ccacgtttat cccgattaca ccggcaccat cgcctttgag
      361 ttgctcggaa tgaggaacgt gccggatctc gcgcgactga accgggaatt gggccggcac
      421 gggcttgccg cgggcgttcc tctggggttc agcaacaact atgcactggc tatgctggag
      481 agccgcgccg aggcgctggg catccggcgc atatccgacc ttcaccatca cccccgcctc
      541 acgctcgcgc tgtcgcagga attcctcaac cgcaaggacg gctggcaggc gttgcgcctg
      601 gcctatcggc tgccgtttgc cgacgcgcgc ggtcttgacc acggcctggc ttacgaggcc
      661 atcgcggccg ggagcctcga cgtcatggat gtctactcga cggaccccaa gatcgaccgc
      721 taccggctgc gtgtgctgca cgatgaccgc ggtttttttc cggcttacga tgcggtcctg
      781 ctgtatcgac aagacctgcc ggagaaattt ccgcagtcgt ggcgggcatt gcagggactc
      841 caggaaaaga tcaccgctgg gcgaatgatg gagatgaatg cggcggctga actatcggcc
      901 gtcccgtttt cccggatcgc ggaggccttt ctcgatggcc gcagcccggc ggggaatacc
      961 ggcgggccac ctgaacggcg atcgctcatg caggcgctgc tggggccgga cttctggcgc
     1021 ctgacgggcg agcatctcgt gctggttttc gcttctctgg cgctcggcgt ggcggccggg
     1081 gtgcccctgg gcgtttgtgc caaccggatg caaggtgcgc gggcctggat actgggggcg
     1141 gcgggcgtgc tgcagaccgt gccgtcgctt gcgctactcg cttttctgat tgccgcgctc
     1201 gaccgtatcg gcacggtgcc cgcgatcatc gcgctgttcc tgtattcgct gctgcccatc
     1261 gtccgcaata cggaaaccgg gcttgccggg gtgccgcgct cgatgaagga ctcggcaacg
     1321 gcgctgggcc tgcgggcggg cgcgcggctg cgatatatcg aattgccgct ggcggcccgc
     1381 accatgctcg ccggcatcaa gacctcggcc gtgatcaacg ttggcaccgc cacgattgct
     1441 gctttcatcg gggctggcgg ctacggcgag cgcatcgttg ccggcctggc ggtgaacgat
     1501 catgccttgt tgctcgcggg cgcaataccg gcggccgcca tggcgcttct cattcagggg
     1561 ttgttcgaca tcctggaccg ccaccagctc ccggggggct cgcgtcctcc ggcttgaaat
     1621 ggccatccgg tctgcaccgt attagcatat cggtcaataa agaagggggg agggctggca
     1681 gtgtctgctt gcctggcgac tgtacggtcg ctgccgtatc cgattcaact tgcactggtg
     1741 gcactgatct acctggttgc cgccaaggct tccctggtat ttgccattgc tcccggttat
     1801 gcaaccgcgg tctggccgcc ctccggcatc gccgtcgcgt cctgcctgct gctgggggag
     1861 cgaatctggc cagcagtctg gctgggcgcc accctcgcca acctgccggt ggccaattcc
     1921 ctgttcgcgg caccggctat cgcctgcggg aacacgctcg aggcggtaac ctgcgcatgg
     1981 ttcgtgcgcc agctcatcgg catcggcccg caattcgggc ggggcgagga cgtgctcaag
     2041 tttgtcgcga ttgccatggc gggttgcgtc atcgccgcca cggtggccgt tcacgtattg
     2101 atcgcggatg gcgcgatcgg atggcaacag ttcgcggcca attggtggac ctggtggcag
     2161 ggagacgtca ctggcatcat tatcgtgacg ccgctgctgc tgagctgggc tttgcacaag
     2221 gacgcttttc cgcccccggg ccgggcgggg gaattcctga tcctggcatt ctcgaccgcg
     2281 gtaaccgttg cggcggtttt cggcgattgg ttcgagaccg gcaaggccca gacgctgacc
     2341 ttcatcgtgt taccgttcat catctggggc gcatttcgtt tcacccagcg cgaggtcacc
     2401 accgtggcag ctatcatctc cgtgattacc atcgagtaca cgctcggggg gcggggtgcc
     2461 tttgtcgccg ggacggtcaa tgaatccctg cttctgctgc tggcattcgt gagcacggtg
     2521 gtcgtaacgg gcctggtgct gaccgcgacg ctggctcaac gcgcacggtg aatggcggag
     2581 cgggcaccaa tacgccccgg gcccgcccct ggcgcccccg acgcggcctg ggttggcctc
     2641 cgtaaccctt agctggcgca acacccagcg agacccgggg tcggggcagg gcctggaagg
     2701 gcgagcctgc ccaccccctg tgaactaatt tttgcctgtc ctgtcagtgg aatagatcgg
     2761 cttcgcacca acc
//
LOCUS       DBNGSG010000433         1562 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1669650, whole genome shotgun sequence.
ACCESSION   DBNGSG010000433 DBNGSG010000000
VERSION     DBNGSG010000433.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1669650"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..398)
                     /locus_tag="AC158Q_11280"
     CDS             complement(45..398)
                     /locus_tag="AC158Q_11280"
                     /inference="COORDINATES: protein motif:HMM:NF027463.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HIR3340399.1"
                     /translation="MRRQKGLTLSGFMVVAIIVVIALLLGFKLAPPYMEFHTLQKLLR
                     QIAAEPHKGEPRGSVRRAFQLKSGIDNITTVNENDLQVTKDGDRVIITAEYTVRVPLI
                     YNISACMDFRATSEQ"
     gene            complement(410..1213)
                     /gene="lepB"
                     /locus_tag="AC158Q_11285"
     CDS             complement(410..1213)
                     /gene="lepB"
                     /locus_tag="AC158Q_11285"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR3340400.1"
                     /translation="MNFALIMFVLLVGTGGIWLLDHYVLRHGRPADQREPWWVEYPKS
                     FFPVILAVFLLRSFLVEPFKIPSGSMLPTLLVGDFILVNKFTYGIRLPIINVKVLDVS
                     QPQRGEVMVFRYPENPSLDYIKRVVGIPGDRIDYRNKRLTINGEAAKLTPAGEYNYVE
                     QGLNFISTRRFDETLDKAQHAILVQPDVPGVQLSGVRPFPFRDNCAYNETGFSCTVPA
                     EHYFLMGDNRDSSSDSRYWGFVPEANIVGKAFLIWWNFDEFGRIGQSIK"
     gene            complement(1321..>1562)
                     /locus_tag="AC158Q_11290"
     CDS             complement(1321..>1562)
                     /locus_tag="AC158Q_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014897649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HIR3340401.1"
                     /translation="LIPRQMFDVAVQAAIGSHIIARETVKAMRKNVLAKCYGGDITRK
                     KKLLEKQKAGKKRMKQVGSVEIPQEAFLAILQVGK"
BASE COUNT          313 a          504 c          409 g          336 t
ORIGIN      
        1 cctatacgtt caccaaacgc ctcgacactc atcgcgccga cgcactactg ctccgaagtt
       61 gcccggaagt ccatgcaggc gctaatgttg tagatcagcg gaacccgtac tgtgtactcc
      121 gcggtgatga tcacgcgatc gccgtccttc gtgacctgaa gatcgttctc gttgacggta
      181 gtgatgttat cgattcccga tttaagctgg aaagctcgcc gtaccgagcc ccgaggctct
      241 cccttatggg gctcggccgc gatctgtctg aggagttttt gcaacgtatg aaattccatg
      301 tatggcggcg cgagcttgaa tccaagcagc aaggcgatca ccacgattat cgccaccacc
      361 atgaacccgg acaaagtcaa gcctttttgc ctgcgcatga gccctcccct catttgatcg
      421 actgccctat ccttccaaac tcatcgaagt tccaccagat caggaatgcc ttgccgacga
      481 tattcgcctc cggcacgaat ccccagtaac ggctgtcgct gctgctgtcc cggttgtctc
      541 ccatcagaaa gtaatgctct gccggcacgg tacagctgaa acccgtctca ttgtaggcac
      601 aattgtcccg gaagggaaac ggccgcaccc cggagagctg cacgcccggt acgtccggct
      661 gcaccaggat cgcgtgctgc gccttgtcga gcgtctcatc gaaccgccgt gtcgagatga
      721 aattcagtcc ttgctcgacg taattatact cgccggccgg cgtgagtttc gccgcctctc
      781 cgtttatcgt aagccgcttg ttgcggtagt cgatacggtc gccgggaatt ccgacaacgc
      841 gcttgatata gtcgagtgaa ggattctccg gatagcggaa taccatcacc tcgccgcgct
      901 gcggctggct cacgtccaga accttgacgt tgatgatggg cagccggatg ccgtaggtga
      961 acttgttgac gagtatgaaa tcccccacca gcagggtcgg cagcatcgag cccgacggga
     1021 tcttgaacgg ctcgaccagg aacgatcgca gcaggaaaac ggcaagtatc actggaaaaa
     1081 agctcttcgg atactcgacc caccacggct cgcgttgatc ggcgggccgg ccgtgccgca
     1141 gcacgtaatg gtcaagcagc catatgcccc ccgtgcccac cagcagcacg aacatgatca
     1201 gcgcgaagtt cacttcgtcc ctttccggca accggtgcag tgtaacggac aaaccgccac
     1261 acctaccgtc ccccccggcc gcccttgctt cttattgtcc atggcgcacc accgaccacc
     1321 ctacttgccg acctggagaa tggcgagaaa cgcctcctgc ggaatttcca cgctccccac
     1381 ctgtttcatg cgcttcttgc ccgccttctg cttttcgagg agctttttct tgcgcgtgat
     1441 gtccccgcca tagcacttgg ccaggacgtt cttcctcatg gcctttaccg tttcgcgggc
     1501 gatgatgtgc gagccgatcg ccgcctgcac ggccacgtcg aacatctggc gcgggatgag
     1561 ct
//
LOCUS       DBNGSG010000434         3085 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4047079, whole genome shotgun sequence.
ACCESSION   DBNGSG010000434 DBNGSG010000000
VERSION     DBNGSG010000434.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4047079"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            263..1666
                     /locus_tag="AC158Q_11295"
     CDS             263..1666
                     /locus_tag="AC158Q_11295"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3340402.1"
                     /translation="MSHESAAGNPYTRGMAEFVSGLKYEAIPEEVRHRLKLLMLDSFG
                     CALYGADLEWSRILQDRLSAIDTTRACGIWGTDKKLSAPHAALVNGTQVQGFELDDVH
                     RAGVLHVGAVVLPALLPLAEMKKGMSGREFLTAAAAGYEIGPRVGLCMGPGHIASGWH
                     SGATLGVFSAAAGAARGLKLDTDRTVHALGIAGTQAAGLMAAQFGAMVKRMHAGRSSQ
                     SGLYGALLAEAGFTGIVNVLESEYGGFCTTMSQSKDQFDLKELTAGLGSTWQAMGVAL
                     KFYACVGTNHTTLDALRDMRRERPFGAKDVKKIVVHGSLVTVNHVGWKYVPQGLTSAQ
                     LNLPYCVATLLLDGDVFVDQFTEAKVADPGRMALAGKVEVVHDPDITALGGKFRHKVH
                     VEAHLNDGSVMKRTVEAARGSEKHFATDAEIVEKFEKLALKALPRAQVEKLRDAVLGL
                     EKLGDAAELARLLARQQ"
     gene            1919..2854
                     /locus_tag="AC158Q_11300"
     CDS             1919..2854
                     /locus_tag="AC158Q_11300"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3340403.1"
                     /translation="MFLLLALALPAQAQNYPDRPIRLIVPLAAGGAMDTIARSVGAKL
                     TDNLGQTVVVDNRGGGGGTVGAGIAMSADADGYTLIMLSATSVIRPLLYKANYVMFRD
                     FTAISQVSAQPYLLVVNPKLPVKTVPELVTHAKANPGKLNYASAGQGSIIHLASELLG
                     VNTGIRMVHVPYKGMGAAYPDLLAGHTQLALASVVSALPHTRSGRLRGVAVTSPQRSK
                     AAPDIPTIAESGVKGYAVTNWYGLLAPAKTPRPIIDRLHRNVVMVLQQPDVAKRFSAD
                     GAEAVHSTPVEFTAHIKAETRKWAGVIKQAGIKGD"
BASE COUNT          589 a          982 c         1011 g          503 t
ORIGIN      
        1 ccgccttgcc ggagcttccg ccggaaaagc agcaataaaa tagctgccaa gacgccaaga
       61 acgccaagag atgcgaatta acaagcactc atgatgagtg cgcattcaag cttgatttga
      121 cgggcaattg aaatgcagag atcgcagata agttgtccga accggaaagt ggattcgtct
      181 gttttcgttc tttatgctcg atgttgcctt tcttggcgtt cttggcgtct tggcggctaa
      241 tttgaaactc tggaattaag cgatgagtca cgaatcggcg gccggcaacc cctacacccg
      301 cggcatggcg gagttcgtct ccggcctgaa atacgaggcg atcccggagg aagtccggca
      361 ccggctcaag ctgctgatgc tcgattcgtt cggctgcgcg ctctacggcg ccgatctcga
      421 gtggagccgc atcctgcagg acaggctgtc cgcgatcgac acgacccgcg cctgcggcat
      481 ctggggcacg gacaaaaagc tctccgcgcc ccacgccgcg ctggtgaacg gcacgcaggt
      541 gcagggcttc gagctggacg acgtgcaccg cgccggcgtg ctgcacgtgg gcgcggtcgt
      601 gctgccggcg ctgcttccgc tcgccgagat gaaaaagggc atgagcggca gggaattcct
      661 gaccgccgcc gccgcgggct acgagatcgg gccgcgcgtg ggcctgtgca tggggccggg
      721 ccacatcgcg tccggctggc attcgggagc gacgctcggc gtgttctccg ccgcggcggg
      781 cgcggcgcgc ggcctcaagc tcgacacgga caggaccgtg cacgcgctcg gcatcgccgg
      841 cacgcaggcc gcgggcctga tggcggcgca gttcggcgcg atggtgaagc gcatgcacgc
      901 ggggcgttcc tcgcaaagcg gcctctacgg cgcgctgctg gcggaggccg ggttcaccgg
      961 gatcgtcaac gtgctggaga gcgaatacgg cggcttctgc acgacgatgt cgcaatcgaa
     1021 ggaccagttc gatctcaagg aactcaccgc cgggctgggc tcgacctggc aggcgatggg
     1081 cgtcgcgctc aagttctacg cctgcgtcgg caccaaccac accacgctcg acgcgctgcg
     1141 cgacatgcgc agggagcggc cgttcggcgc gaaggacgtg aagaaaatcg tcgtgcacgg
     1201 ctcgctggtc acggtcaacc acgtcggctg gaagtacgtg ccgcaggggc tcacttcggc
     1261 gcagctcaac ctcccctact gcgtcgcgac gctgctgctc gacggcgacg tcttcgtgga
     1321 ccagttcacc gaggccaagg tggccgatcc cgggcgcatg gcgctcgccg ggaaggtcga
     1381 ggtggtgcac gatcccgaca tcaccgcgct cggcggaaag ttccgccaca aggtccacgt
     1441 cgaagcgcat ctcaacgacg gcagcgtgat gaagcgcacc gtggaagccg cgcgcggcag
     1501 cgagaagcat ttcgcgaccg acgcggagat cgtggagaag ttcgagaagc tcgcgctgaa
     1561 agcgctgccg agggcgcagg tagagaagct gcgcgacgcc gtgctggggc tggagaagct
     1621 cggcgacgcg gccgagctcg cgcggctgct ggcccgtcaa caataatgga caggattaag
     1681 gaatctctgc ataagtcatc cccgcgcagt cggggatcca gtaacgattt gtttaccctg
     1741 gattcccgcc ttcgcgggaa tgacgaaaaa cgactattgc agagcatcct taacaggatt
     1801 cacaagatac tacccgagag ccgttcgttg ctacaggaca cctgcaaccc tgccaaatag
     1861 aacagggtta atccgaggag gttttatgag gcccgttaat cttgttgatc ctgtctattt
     1921 gttcttgctg ctcgcgcttg ccctgccggc gcaggcgcag aactaccccg accggccgat
     1981 ccggctgatc gtgccgctcg cggccggggg cgcgatggac accatcgcgc gcagcgtcgg
     2041 cgcaaagctc accgacaacc tcggccagac cgtggtggtg gacaaccgcg gcggcggcgg
     2101 cggcaccgtc ggcgcgggaa tcgccatgtc cgcggacgcc gacggctaca cgctcatcat
     2161 gttgagcgcg acctcggtga tccgcccgct gctctacaag gcgaattacg tgatgtttcg
     2221 cgatttcacc gccatctccc aggtcagcgc gcagccgtac ctgctggtgg tcaacccgaa
     2281 gctgccggtg aaaacggtgc ccgaactggt cactcacgcg aaggccaacc ccggcaagct
     2341 caattacgcg tctgccggcc agggcagcat catccacctc gcgtcggaac tgctcggcgt
     2401 caataccggg atccgcatgg tgcacgtgcc ctacaagggc atgggcgccg cctaccccga
     2461 cctcctcgcc ggccacacgc agcttgccct ggccagcgtc gtgtcggccc tgccgcacac
     2521 gcgctccggc cgcttgcgcg gcgttgccgt gaccagcccg caacggtcga aggccgcgcc
     2581 cgacatcccg acaatcgcgg aatcgggcgt gaagggatac gccgtgacca actggtacgg
     2641 cctgctcgcg ccggcgaaga cgccgcgccc gatcatcgat cgcctgcacc ggaatgtcgt
     2701 gatggtgctc cagcagccgg acgtcgcgaa acgtttctcc gcggacggcg ccgaggccgt
     2761 ccacagcacg ccggtggaat tcacggccca catcaaggcc gaaacccgga agtgggcggg
     2821 ggtgatcaag caggcgggca tcaaaggcga ttgaagcgtg ataagtgacg ggtgataagt
     2881 gataagttac ccgctgcgcg gaaactgatt cctgtcaccc atcacttatc acctatcact
     2941 gttccggagg aacgaaaaat ggtccaggca gtcgcaggcg agcaggcgat gaaagggcgc
     3001 cgcgtgatca agtcgcgcgg cacgggcgag gagcactggc gcaccgattt cctgggcagg
     3061 ccgggcgacg gcggcgcgat cctcg
//
LOCUS       DBNGSG010000435         2658 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_481643, whole genome shotgun sequence.
ACCESSION   DBNGSG010000435 DBNGSG010000000
VERSION     DBNGSG010000435.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_481643"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="AC158Q_11305"
     CDS             complement(<1..492)
                     /locus_tag="AC158Q_11305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MsnO8 family LLM class oxidoreductase"
                     /protein_id="HIR3340404.1"
                     /translation="MRLSVLDQSPVISGHAPARAVHETIRLVKAVERLGYHRYWLAEH
                     HSIAALADPCPEILLARIAAETTRIRVGTGGILLPYYSPFKVAEQFRMLEALYPGRID
                     LGIGRAPGGDPVTAMAMGEGRYPGAEHFPEQVQYLVAYLDGTLPAEHPFAKVKVMPAG
                     PGAP"
     gene            669..2084
                     /locus_tag="AC158Q_11310"
     CDS             669..2084
                     /locus_tag="AC158Q_11310"
                     /inference="COORDINATES: protein motif:HMM:NF017135.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase"
                     /protein_id="HIR3340405.1"
                     /translation="MATVQQSASTPSPLSALERLRHWWHRLAATVFPGPAAADRVTAR
                     LIGQLKPLLEDSRVLRGGEISARSRAERIAALYRGADMPQRAAILNLITHEFAPDRGE
                     LEQAISAIQAAVDDAGLSRAEARLRVALDAPRAKFLTQFNLLSDGVKFLVDLRADLLE
                     LLPREPALEVLQVELDGLLERWFDPGFLELRRISWQSPALVLEKLIAYEAVHPIESWN
                     DLRNRLDTDRRCYAFFHPRLPNEPLIFVEIALLKGLPGSVQIVLDQNAPVQDPLAADT
                     AIFYSISNTQKGLRGISFGNLLLKRVIEDLQRDLPKLQVFATLSPLPGFRKWLDHALA
                     DGRALVPAADTAKLAAIIGHDDPATALAVALARADWPGDTLIADELREPMEKLCARYL
                     LQEKSGTQPLDSVAQFHLHNGARVSRIFWLADVSARGMRQSYGLMASYRYDLSEVDRN
                     HEQFLENGHIAAARRVQRLLN"
     gene            complement(2215..>2658)
                     /locus_tag="AC158Q_11315"
     CDS             complement(2215..>2658)
                     /locus_tag="AC158Q_11315"
                     /inference="COORDINATES: protein motif:HMM:NF013046.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HIR3340406.1"
                     /translation="DGLYYPYEEDPDLIYADIHQDGRTLYPGTGHAHETGKGAAKGAK
                     LNIPMLPGMGDNDFLEVWPRVETHLRRFKPEFILFQCGADSLAGDPLAMLQYTPAAHA
                     HAAKRLCVLANELAGGRIMGFGGGGYNRGNLAAAWCGVLDEFIKA"
BASE COUNT          469 a          864 c          858 g          467 t
ORIGIN      
        1 cggcgcgccc ggtccggccg gcatcacctt caccttggcg aacgggtgtt ccgccggcag
       61 cgtgccgtcg aggtaggcga ccagatactg cacctgctcc ggaaagtgct ccgcgcccgg
      121 gtaacgcccc tcgcccatgg ccatcgccgt gaccgggtct ccgccgggag cgcgaccgat
      181 cccgaggtcg atgcggccgg gatagagcgc ctcgagcatg cggaactgct ccgccacctt
      241 gaacggactg taatagggca gcaggatgcc gccggtcccg acccggatgc gcgtcgtctc
      301 cgcagcgatg cgcgcgagca gaatctcggg acagggatcg gcgagggcgg cgatcgagtg
      361 gtgctccgcg agccagtagc ggtggtagcc cagccgctcc accgctttca ccagccggat
      421 ggtttcatgc acggctcgtg ccggcgcatg gccgctgatg acaggagact ggtcgagaac
      481 gctcaggcgc attggatgtc aaatctaaca tagggacagg gtgtcattcg cactcccccg
      541 gtgcgcccgg cagaacggaa tgtctgtttt cggcaacgcg catgccggaa agactggtac
      601 aggcacccga tcctctttga actgcagcag aatcagacta acatcgtatc ggaacggcaa
      661 gtcgggaaat ggcgacagtg caacagtcag caagcacacc gagtccgctc tccgccctgg
      721 agcgattgcg tcactggtgg caccggcttg cggctacggt gtttcccggt cctgcggccg
      781 cggaccgggt gaccgcgcgc ctgatcgggc aactcaagcc gctgctggag gatagccggg
      841 tactgcgcgg cggcgaaatc tcggcgcgca gccgcgccga gcgtatcgcg gccctctatc
      901 gcggcgcgga catgccgcag cgtgccgcca tactcaacct gatcacccat gagttcgcgc
      961 ccgaccgcgg cgaactggaa caggccatct cggcaatcca ggccgcggtg gatgatgccg
     1021 ggctctcccg ggcggaagcg cgcctgcgcg tcgcgcttga tgccccacgc gcgaagttcc
     1081 tgacgcaatt caacctgctg tcggatggcg tgaagttcct ggtcgacctg cgcgcggacc
     1141 tgctcgagct tctgccccgg gagcccgccc tcgaggtgct gcaggtcgag ctcgacggac
     1201 tgctggagag atggttcgac cccgggttcc tggagttgag gcgcatctcc tggcaatcgc
     1261 cggcactcgt cctcgaaaaa ctcatcgcct acgaggccgt ccacccgatc gaatcgtgga
     1321 acgatctgag aaaccggctc gataccgacc gccgttgcta cgcgttcttt cacccgcgcc
     1381 tgcccaacga gccgctgatc ttcgtcgaaa tcgcgctctt gaagggcctg cccggcagcg
     1441 tgcagatcgt gctcgaccag aatgcaccgg tgcaggatcc gctcgccgcg gacacggcga
     1501 tcttctactc gatttcgaac acgcagaagg ggctgcgcgg catcagtttc ggcaacctgc
     1561 tgctgaagcg agtaatcgag gacctgcagc gcgacctgcc gaagctccag gtctttgcca
     1621 cgctctcgcc gctgccaggc ttccgcaaat ggctggacca cgcgctcgcc gacgggcgtg
     1681 cgctggtgcc ggcagcggac accgcgaagc ttgccgcgat aatcggacat gacgatcctg
     1741 ctactgcgtt agccgtagcg ctggcgcgcg ccgactggcc gggcgacacc ctgattgcgg
     1801 acgagctgcg cgagccgatg gagaaactct gtgcacgcta tctgctgcag gaaaaatcgg
     1861 gcacgcagcc gctcgattcg gtcgcgcagt tccacctcca caacggcgcg cgcgtgagcc
     1921 gcatcttctg gctggccgat gtttcggctc gcggcatgcg tcaatcctac ggactgatgg
     1981 cgagctaccg atacgacctg tccgaggtgg acaggaacca cgagcagttc ctggagaatg
     2041 gccacatcgc cgccgcgcgg cgagtacagc ggctactgaa ttagccaaaa aagcacaaag
     2101 cgaaccgcca ggacgccaaa aagatcgaat tttgtcctgt tattcttggc ggccttggcg
     2161 tcttggcggt tcatattaag tagtttgaat ttcttggcgt cctggcggct gatttcaagc
     2221 cttgatgaac tcgtccagca ccccgcacca cgcggcggcg aggttgccgc ggttatagcc
     2281 gcccccgccg aaacccatga tgcgcccgcc ggccagttcg ttcgccagca cgcaaagacg
     2341 cttcgcggcg tgggcgtgcg cggccggggt gtactgcagc atcgcgagcg ggtcgccggc
     2401 gaggctgtcg gcgccgcatt gaaacaggat gaactccggc ttgaaccggc gcaggtgggt
     2461 ctcgactcgc ggccagactt cgagaaagtc gttgtccccc atgcccggca gcatcggaat
     2521 attgagcttg gcgccctttg ccgcgccttt gccggtttcg tgcgcgtggc cggtgccggg
     2581 gtataacgtg cgcccgtcct ggtggatgtc ggcgtagatg agatccgggt cctcctcgta
     2641 gggatagtag aggccgtc
//
LOCUS       DBNGSG010000436         5987 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_482339, whole genome shotgun sequence.
ACCESSION   DBNGSG010000436 DBNGSG010000000
VERSION     DBNGSG010000436.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5987)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5987)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5987
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_482339"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..1886)
                     /locus_tag="AC158Q_11320"
     CDS             complement(144..1886)
                     /locus_tag="AC158Q_11320"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HIR3340407.1"
                     /translation="MAKAKPKTGMRKGLTAYGDEGFSLFLRKAFIKAMGYTDDALERP
                     IIGITNTFSGFNACHRTVPELIEAVKRGVMLAGGLPVEFPTITLHESFAHPTSMYLRN
                     LMAIDAEEMIRAQPLDAVVLIGGCDKTVPALLMGAASVNVPAILMVTGPMITGEHKGE
                     RLGACTDCRRYWARFRASEIDEQEINAVNDQLCPSAGTCMVMGTASTMALCTEALGMM
                     LPQSACIPAVMAERMRNAEASGARAVGLAKERLTPDRIMTPAAFENALRMLLAIGGST
                     NAIVHLAAMAGRLGIALDLDAFDRMGHKTPVLVDLKPTGQHYMEDLYKAGGAATILRE
                     LKPLLNLDALTVTGRSLGENLAATPPSWSGQQVVRPSKNPIFREGGIAFLRGNLAPGG
                     AIIKQSAMDPKFMRHEGRAVVFESLEDLANRIDDPALDVTAKDVLVLKNAGPRGAPGM
                     PESGYLPIPKKLAQKGVKDMVRISDARMSGTAFGSIVLHVTPESAAGGPLALVKNGDR
                     IRLDVPRRRLELKVSPAELAKRKKAWRAPRARAEDRRGYRKLFMETVTQADRGCDFDF
                     CVPEMKSRVPRAKR"
     gene            complement(2065..2994)
                     /locus_tag="AC158Q_11325"
     CDS             complement(2065..2994)
                     /locus_tag="AC158Q_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340408.1"
                     /translation="MPDGLERERATRCEFAGINWGPVMTGAFDLMFVAVPAGIALSSV
                     VYFAGRVLLDRRHGTSSFGGQGGLAIGLVVLGFFLYQAWQFARPARTARLVSSPTTQP
                     PAARADASKAGRAEPGQDARGAAAAGQEDDESRLKTTLASIEKESGPDHPAAGRVHFR
                     LSLVYERHKQFAEQEQALLAALEVLEKHPAAAVRKALGAEGGTLDKEIVARRLGDFYW
                     DRRRYDRAYVHYEKAYRYTGEIPLSDFERNRRLARNSAGRMAGACTQGDWDVADRAMK
                     ELKERMANVDAETRKQLEYWVRTGEPRLAARKC"
     gene            complement(2925..3842)
                     /locus_tag="AC158Q_11330"
     CDS             complement(2925..3842)
                     /locus_tag="AC158Q_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340409.1"
                     /translation="MAAPSNRLLSVQNPKSLAGMWIFTVLWCGISFTVFWAFAIASRQ
                     TGGAIVGGLFSLLGLVMLFGSVKATLEYLKFGRVHLALEGEQPAAGRSFSARLDLPGE
                     AGAAATIHAELVCVQTTWSRGSKGGPSKSERDAWTRKNVFPVRRGPVSGYAMLKFDVP
                     ADQPASDLPGEGPPDAMLEVGHPAGIEIGRSYYRWELRVKADVPGIDLERTFKLRVAA
                     GPQGAGPRPAGAAPRLDPKFGAELERRVEARQEQDRRLGMWCAFIGFAPIVAPFAIMG
                     LAVGLAGCPMGWNASAPPGASSPASTGAR"
     gene            complement(4027..4959)
                     /locus_tag="AC158Q_11335"
     CDS             complement(4027..4959)
                     /locus_tag="AC158Q_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 family protein"
                     /protein_id="HIR3340410.1"
                     /translation="MAGDARVPGKGWYWLAGAVALAGVVAMAALIAWFVLTLDKGAQF
                     LAPGRHAVRLDRPGTYLVWNDYRTVFQGRSYDESEKLPSGVRISVLDRAGGRSLEVSA
                     SLGATSSIGDTHSVSVSQFVIERPGSYEIAVEGDFPQRVFSVSRNFVPGLLWTIFGAF
                     ALLFGGFGVATAIAAWAFIKREDRQGAGRVQAAAVPDAPAGAPAREQSLKNIAAIVYG
                     LQLASFLVGITIIAAVIVNYVKRGEVEGTWLESHFRWQIRTFWYSLLWFGIGLASLIV
                     VIGFFVLMAAAIWLLYRAVKGWVALEEGKAMYQG"
     gene            5031..5762
                     /locus_tag="AC158Q_11340"
     CDS             5031..5762
                     /locus_tag="AC158Q_11340"
                     /inference="COORDINATES: protein motif:HMM:NF015682.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HIR3340411.1"
                     /translation="MPRTPLGKLPREAIRLLELPRLPPEILSGFKVLVDLTGTISDAM
                     DQLGIVGVVPAYVLPPVTPGTRVVGPAVTVRNVQRVAQIHKAATVDKTNTQGETEAHN
                     LAEPGDVLVIQGVMGCSNMGGQSASIAKRQGFIGAIVDATVRDPDGYRGMDWPVWCRG
                     FTPITGKWRMETVEVNGVVEICGVQVRPGDLVCADEAGVAFVPFARAADVLEIARGID
                     AGDTKRKADIDKGASVADLVTRKYK"
BASE COUNT          996 a         2067 c         1911 g         1013 t
ORIGIN      
        1 cgggtcattc aaagaacaat tattattagc cgcaaagtcg cggagactcg gagaaaatca
       61 atgaaatatt tgattttgcc gatcctgtga atcctgtcta ttccgttttg tatttcttgg
      121 cgccttggcg gctaatttgg tcgtcagcgc ttcgcgcgcg gcacgcgcga tttcatctcc
      181 ggcacgcaga aatcgaaatc gcagccgcgg tcggcctgag tcaccgtttc catgaacagc
      241 ttgcggtagc cgcggcggtc ctctgcgcgc gcgcgggggg cacgccaggc ctttttccgt
      301 tttgcaagtt cagccgggct taccttcagc tcgagccggc gccggggcac gtcgagccgg
      361 atgcggtcgc cgttcttcac cagcgcgagc ggcccgcccg ccgcggattc cggcgtcacg
      421 tgcagcacga tggaaccgaa cgcggtgccg ctcatgcgcg cgtccgaaat ccgcaccatg
      481 tccttcacgc ccttctgcgc gagcttcttc gggatcggga gatagcccga ctccggcatg
      541 cccggcgcgc ccctggggcc ggcgttcttc agcaccagga cgtccttcgc ggtgacgtcg
      601 agcgccgggt cgtcgatgcg attggcgagg tcctccagcg actcgaacac caccgccctg
      661 ccctcgtggc gcatgaactt cggatccatc gccgactgct tgatgatcgc gccaccgggc
      721 gcgagattgc cgcgcagaaa tgcgattccg ccctccctga agatcgggtt cttcgaggga
      781 cgcacgacct gctgccccga ccaggacggc ggcgtggccg cgagattttc gcccaggctc
      841 cggccggtca cggtcagcgc atcgaggttg agcagcggct tcagctcgcg caggatggtg
      901 gccgcgccgc cggccttgta gagatcctcc atgtaatgct ggccggtcgg cttcaagtcc
      961 accagcaccg gcgtcttgtg gcccatgcga tcgaacgcat cgaggtccaa cgcgatgccc
     1021 agccggcccg ccatcgccgc gagatgcacg atggcattgg tcgaaccgcc aatcgcgagc
     1081 agcatcctga gcgcgttctc gaacgccgcc ggcgtcatga tccggtccgg ggtcagccgt
     1141 tccttcgcga gccccaccgc gcgcgcgccg ctcgcctcgg cgtttctcat gcgctccgcc
     1201 atcaccgccg gtatgcacgc gctctgcggc agcatcatgc cgagcgcctc ggtgcagagc
     1261 gccatggtgg aagccgtgcc catcaccatg caggtgccgg ccgaaggaca gagctggtcg
     1321 ttgaccgcat tgatctcctg ctcgtcgatt tcggacgcgc ggaacctggc ccagtagcgg
     1381 cggcagtcgg tgcaggcgcc gaggcgctcg cccttgtgct cgccggtgat catcggcccg
     1441 gtcaccatca ggatcgccgg cacgttgacg ctcgccgcgc ccatcaggag ggccggcacc
     1501 gtcttgtcgc agccgccgat cagcaccaca gcatccagcg gctgcgcgcg aatcatctct
     1561 tccgcgtcga tcgccatcag gttgcgcagg tacatgctgg tcgggtgtgc gaacgactcg
     1621 tgcagcgtga tcgtcgggaa ctcgaccggc agcccgccgg cgagcatcac accgcgcttg
     1681 accgcctcga tcagctccgg gacggtgcga tggcaggcat tgaagccgct gaaggtgttg
     1741 gtgatgccga tgatgggccg ctcgagcgcg tcgtccgtgt agcccatcgc cttgatgaag
     1801 gccttgcgca gaaacagcga gaagccctcg tcgccgtagg cggtgaggcc ctttctcatt
     1861 ccggtcttcg gttttgcttt tgccatcggg atatcattcc ttggtgcttc ggactgcgtg
     1921 actcgttact cgttgctcgt tgctcgttgc agccgatcgc tgagacctta ccagaagttc
     1981 aacaccagaa acgagaaacg agtaacgaat cactttttcc gcaagccggc acaataacgg
     2041 atgccggctg cggcgcgcaa gccgtcagca cttgcgcgcg gcgagccgcg gctcgcctgt
     2101 gcggacccag tattccagtt gcttgcgggt ttcggcgtcc acgttggcca tgcgctcctt
     2161 caactccttc atcgcccggt ccgccacgtc ccagtcgccc tgcgtgcacg cgccggccat
     2221 gcgccccgcg gagttgcggg cgagccgccg gttgcgctcg aagtcgctga gcgggatttc
     2281 gccggtgtag cggtacgcct tttcgtaatg gacgtacgcc cgatcgtagc ggcgccggtc
     2341 ccaatagaaa tcgccgagcc ggcgggcgac gatctccttg tccagcgtgc cgccttccgc
     2401 gccgagcgcc ttccggactg cggcagcggg gtgcttctcc aggacttcga gcgcggcgag
     2461 cagcgcctgc tcctgctcgg cgaattgctt gtgccgctcg tagacgaggc tcaagcgaaa
     2521 gtgcacccgc ccggcggcgg ggtgatcggg accgctctcc ttctcgatgg aggccagcgt
     2581 cgtcttcagg cgcgattcat cgtcttcctg cccggcagcc gctgcgcccc tggcatcctg
     2641 ccccggttcc gcgcgcccgg ctttggaggc gtccgcgcgc gcagccggag gttgggtggt
     2701 cggggacgat accagccgcg cggtcctggc cgggcgcgcg aactgccacg cctggtagag
     2761 gaagaaaccc agcacgacga ggccgatcgc gagccccccc tggccgccga agctgctcgt
     2821 cccgtggcgc cggtccagca gaacccgccc cgcgaagtag acgacggagg aaagcgcaat
     2881 gcccgccggc accgcgacga acatgagatc gaacgcgccg gtcattaccg ggccccagtt
     2941 gatgccggcg aactcgcacc gggtggcgcg ctcgcgttcc agcccatcgg gcaaccggcg
     3001 agccccaccg ccaggcccat gatcgcgaag ggcgccacga tcggcgcaaa tccgatgaac
     3061 gcgcaccaca tgccgaggcg gcgatcctgc tcctgccgtg cttcgacgcg ccgctcgagt
     3121 tccgcgccga acttgggatc cagccgcggc gcggcgcctg caggccgcgg acccgcgccc
     3181 tgcggccccg ccgccacgcg cagcttgaac gtccgctcga gatcgatgcc gggcacgtcc
     3241 gccttcacgc gcagctccca gcggtaatag ctgcggccga tctcgattcc ggctgggtgg
     3301 ccgacttcga gcatggcatc cggtggccct tcacccggca aatcggaggc cggctgatcc
     3361 gccggcacgt cgaacttcag catcgcgtag cccgacaccg ggccgcgccg gacggggaac
     3421 acgtttttcc gggtccacgc atcccgctcg ctcttggacg gcccgccttt cgatccccgc
     3481 gaccaggtcg tctgcacgca caccagttcg gcgtggatcg tcgccgccgc gcccgcttcg
     3541 cccgggagat cgagccgcgc gctgaaactc cggcccgccg ccggctgctc gccctccagc
     3601 gcgaggtgga cccggccaaa cttcaggtac tccagcgttg cctttaccga cccgaagagc
     3661 atgacgaggc cgagcagaga gaacaggccg ccgacaatgg cgccgccggt ctggcgggac
     3721 gcgatggcaa acgcccagaa gaccgtgaac gagatcccgc accacaggac cgtgaagatc
     3781 cacatccccg cgaggctctt cgggttctgg acggacaaca acctgttcga tggtgcggcc
     3841 atgttcgatt gggaaaagtg actcgtttct cgtgaattgg gtgtcgagtg tcgggtgtcg
     3901 agtgtcgggt gtcgagtgtc gagtgtcgag tggcgggtgg cgggtgaaga ataacgacag
     3961 cggtttaccc ctcgacactc aatcctcgtc ccccgtttcc ccctcgacac tcattccttg
     4021 acactctcat ccctggtaca tggccttgcc ttcctcgagc gcgacccagc ccttgaccgc
     4081 gcggtacagc agccagatcg ccgcggccat cagcacgaag aagccgatca cgacgatcag
     4141 cgaggctagc ccgataccga accagagcag gctgtaccaa aaggtgcgga tctgccagcg
     4201 gaaatgggat tcgagccagg taccctcgac ttccccgcgc tttacgtagt tgacgatcac
     4261 cgctgcgatg atcgtgatcc cgacgaggaa cgaagccagc tgcaatccat aaacgatcgc
     4321 ggcgatgttt ttgagcgact gctcgcgcgc gggcgctcct gcgggcgcgt cgggcactgc
     4381 tgccgcttgc acgcgcccgg ccccctgtct gtcttcgcgc ttgatgaacg cccaggcggc
     4441 aatagcggtc gccacgccga atcccccgaa caggagcgcg aacgccccaa atatcgtcca
     4501 caacaggccg ggaacgaaat tccggctgac ggaaaacacc cgctgcggaa agtcgccctc
     4561 gacggcaatc tcgtagctgc cgggccgctc gatcacgaat tgcgaaaccg aaacgctgtg
     4621 agtgtcgccg atgctcgacg tcgctccgag gctcgccgag acctcgagtg accggccgcc
     4681 cgccctgtcg agaacgctga tgcgcacgcc ggaagggagc ttctcggact cgtcgtagct
     4741 gcgcccctgg aacacggtcc ggtagtcgtt ccacaccagg taggtcccgg gccggtccag
     4801 tcggacggcg tgccggccgg gcgcgagaaa ctgcgctccc ttgtccagcg tcagcacgaa
     4861 ccacgcgatg agcgcggcca tcgcgacgac gcccgcgagc gcaaccgccc ccgcgagcca
     4921 gtaccagcct ttgcctggaa cgcgggcgtc ccctgccatg tttcctcccc tgtggaagca
     4981 ccgcttcgac caatgatact ctccggtatt cgacacgagg aacacgacac gtgcccagga
     5041 ctcctctcgg caagctgccg cgcgaggcca tccggttgct cgagctgccg cgcctgccgc
     5101 cggaaatact ctcgggtttc aaggtgctgg tggatctcac cggcacgatc tcggatgcca
     5161 tggaccagct gggcatcgtg ggcgttgtgc ccgcctacgt gctgccgccg gtgacgcccg
     5221 gcacgcgcgt cgtcggcccc gcggtcacgg tgcgcaacgt ccagcgcgtc gcgcagatcc
     5281 acaaggcggc gacggtggac aagaccaaca cgcagggcga gaccgaagcg cacaacctgg
     5341 ccgaacccgg cgacgtgctg gtgatccagg gcgtcatggg ctgctcgaac atgggcgggc
     5401 agtcggcgag catcgccaag cgccagggct tcatcggcgc gatcgtggac gcgacggtgc
     5461 gcgatcccga cggctaccgc ggcatggact ggccggtctg gtgcaggggc ttcacgccga
     5521 tcaccggcaa gtggcgcatg gagacggtgg aagtgaacgg cgtggtcgag atctgcggcg
     5581 tgcaggtgcg tcccggtgac ctggtgtgcg ccgacgaggc cggcgtcgcg tttgtcccct
     5641 ttgcccgcgc cgccgacgtg ctggagatcg cccggggaat cgacgccggg gacacgaagc
     5701 gcaaggccga catcgacaag ggcgcgagcg tggccgacct ggtcaccagg aagtacaagt
     5761 aaggcagggg cggcgaatcg tcctggcggc atccaccatc cacgcccaaa gcaaccggca
     5821 ttccgccatg gcgcccatcc gcatctccgt catcacttac aacctctggc tgacgcaacg
     5881 ctggcgcctg cgcgcgccgg cgctgaagga attcctccgg ttgtacgatc cggatctctt
     5941 gtgcgtgcag gagctgcagc cgcgctcgca gcgcctgatt gacaaga
//
LOCUS       DBNGSG010000437         8323 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_652616, whole genome shotgun sequence.
ACCESSION   DBNGSG010000437 DBNGSG010000000
VERSION     DBNGSG010000437.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 8323)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8323)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8323
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_652616"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /gene="dacB"
                     /locus_tag="AC158Q_11345"
     CDS             complement(<1..999)
                     /gene="dacB"
                     /locus_tag="AC158Q_11345"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine endopeptidase"
                     /protein_id="HIR3340412.1"
                     /translation="MTEIKTSWVADRAGPIQRSLLAILGLFALGLPPGASAQLPEPVA
                     RALVKAGIPEASAALYIHEIGSERPLFTAGADRAMNPASTIKLLTTYAALDLLGPAFS
                     WTTEAYAAGTLKDGVLTGDLVIKGRGDPKLTLENFWLLLRELRGRGLREIQGDLVIDR
                     SYFAPGLFDPARFDEQPTRPYNTGPDALLVNFKAVRLQFIPDADARAVRIVAEPPLPQ
                     VRIVNNLVLDATGFCGDWASKLRLDAQGDSTAARFAFNGTFPASCGERERYFSVLGHS
                     QYVLAVFRELWRELGGTLSGTVREGAAAADARLLASMKSPALSEVVRDINKFSNNVM"
     gene            complement(1026..2357)
                     /locus_tag="AC158Q_11350"
     CDS             complement(1026..2357)
                     /locus_tag="AC158Q_11350"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     S-adenosyl-L-methionine:8-amino-7-oxononanoate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HIR3340413.1"
                     /translation="MQTSTRNALLLKRSLAAVWHPCTQMKQHEWLPLVPVARGEGCWL
                     YDFDGRRYLDAISSWWVNLFGHCNPRINAAIADQLGKLEHVLLAGCSHEPAVTLSERL
                     ARATGGALGHCFYASDGASAVEIALKMSFHYWRNAGRPAKTGFVSLAGSYHGETLGAL
                     AVTDVALFKDTYAPLLMASRQVPSPDWRLAEQGESARDFAERCAQALERHLERHHGET
                     AALIVEPLVQGASGMAMHDAQYLKRARALCDRYGVHLIADEIMTGFGRTGSLFAHQQA
                     AIAPDFLLLSKGITGGYLPLSCVMTRDAVYQAFYDDEVARGFLHSHSYTGNALACRAA
                     LATLDIFEEDRVIEANRERSARITAAAQPIAQHPRVRHFRSTGMILAFDVDTADAGFA
                     RRFFASALERELLLRPLGNTVYFMPPYVIGPEEIELLARRTADILDALPAG"
     gene            2637..3278
                     /locus_tag="AC158Q_11355"
     CDS             2637..3278
                     /locus_tag="AC158Q_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HIR3340414.1"
                     /translation="MQILELIEDQISATGLPLVGITLAAVPCPDTPVILTLHWHGFIK
                     ERLADAEQAAPVSYTPVPSSALQLNDRWRDLVAVDWAAMEAAWELGAWDVARAERKGC
                     LRPGAPSTEALECLQAFGAFPYGINGNQVVVSDAPDADELVQLAAARGYLMWLFRPVS
                     GGIWAEVADDATLTPKGRRPSPCPHRPIPPACDGNRKTIYRFGVPASASGDRR"
     gene            3480..3773
                     /gene="groES"
                     /locus_tag="AC158Q_11360"
     CDS             3480..3773
                     /gene="groES"
                     /locus_tag="AC158Q_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HIR3340415.1"
                     /translation="MKIRPLHDRVIVKRLEEERKTASGIVIPDTAAEKPDQGEVIAVG
                     KGKILEDGKVRPLDIKVGDKILFGKYSGQTVKVKGDELLVMREEDIMGVIEAK"
     gene            3802..5439
                     /gene="groL"
                     /locus_tag="AC158Q_11365"
     CDS             3802..5439
                     /gene="groL"
                     /locus_tag="AC158Q_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561123.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIR3340416.1"
                     /translation="MSAKDVKFHDFARSKIVAGVNILADAVKVTLGPKGRNVVLERSF
                     GAPTVTKDGVSVAKEIELKDKFENMGAQMLKEVASKTSDVAGDGTTTATVLAQAIVQE
                     GMKYVAAGMNPMDLKRGIDKAVEAIVAELKKISKPCTTSKEIAQVGAISANADLEIGK
                     IISDAMEKVGKEGVITVEDGKSLNNELEVVEGMQFDRGYLSPYFINSAEKQMSMLENP
                     YVLLYDKKISGIRELLPVLEQVAKAGRPLLIIAEEVEGEALATLVVNNIRGILKTCAV
                     KAPGFGDRRKAMLEDIAILTGGVVISEETGMQLEKITLKELGQAKRIEIGKENTTIID
                     GNGDTKTIEGRVKSIRAQIEEATSDYDKEKLQERVAKLAGGVALIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVPGGGVAYLRARVNAKPKAENHDQEAGIKIVMRALEEPL
                     RWITANAGDEPSVVVNKVVEGKGNFGYNAQSGQYGDMVEMGVLDPTKVTRYALQNAAS
                     VAALILTTDAMVAELPKDESHAGHGHGGMGGMGGMDM"
     gene            5906..5981
                     /locus_tag="AC158Q_11370"
     tRNA            5906..5981
                     /locus_tag="AC158Q_11370"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5939..5941,aa:Phe,seq:gaa)
     gene            complement(6060..6992)
                     /locus_tag="AC158Q_11375"
     CDS             complement(6060..6992)
                     /locus_tag="AC158Q_11375"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014757.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - (3S)-3-hydroxyacyl-CoA
                     dehydrogenase (NAD+) activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3340417.1"
                     /translation="MPKQPSAVAIIGGGIMGGDIAIIFAAGGWDVHVMSPSQKTRDAL
                     PARIAAGLKKLGAPQDRAASVKTCAELEALPWKDIGLVVEAAVENLALKQKLFGQIEA
                     LARPDIALTSNTSNFPIGEVGKGLKRRGRVAGLHFFMPAHLVPLVEIVSADATDPKVA
                     EGLMSLMKDLGKAPIWVKKDVPGFVGNRLQHAMMREALYLIGDGIISPEGIDDAVRYG
                     FGFRFIACGPMLQKEMSGWDTNAIVGAALYPHLYSQTAYPPFVQDMVDKGHLGMKTKH
                     GFWQWTDEAIAKEKARIERCLQAGMEILKADGGK"
     gene            complement(7120..7995)
                     /locus_tag="AC158Q_11380"
     CDS             complement(7120..7995)
                     /locus_tag="AC158Q_11380"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008163432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR3340418.1"
                     /translation="MHSSRLRTTRFFAALFAALALALAGTAPAQGQEYKPSVGQEGKD
                     VIWVPTPQALVERMLDMAKLTPQDIHYDLGSGDGRTVITAAKRGAQAVGVEFNPDMVK
                     LSERAAAKEGVADRAKFINGDIFQTDFSHANVLTLYLLPSLNLKLRPTILKMKPGTRV
                     VSHAFSMDDWQPDQTDNVEGRTAYLWIVPAQVEGTWRWSVAGSGQKEYELVLRQQFQQ
                     VEGLVRLDNGRMGQLRNVKLAGDQISFSIHELAGTSGTIRRDYTGRVSGNTIQGVFKL
                     TDTGAEVKWAATRAK"
BASE COUNT         1509 a         2592 c         2763 g         1459 t
ORIGIN      
        1 catcacgttg ttggagaact tgttgatgtc gcgcaccacc tccgagagcg ccggcgattt
       61 catgctggca agcaggcgcg cgtcggccgc cgcggcgccc tcacgcacgg tgcccgacag
      121 cgtgccgccc agctcgcgcc agagttcgcg gaacaccgcc agcacgtact gggaatgacc
      181 gagcacgctg aaatagcgct cgcgctcgcc gcagctcgcc gggaacgtgc cgttgaaggc
      241 gaaacgcgcc gcggtcgagt cgccctgggc gtcgagccgc agcttgctcg cccagtcgcc
      301 gcagaaaccg gtcgcgtcga gcacgaggtt gttgacgatt ctcacctgag gcagcggcgg
      361 ttcggcgacg atgcgcacgg cgcgcgcatc cgcatcgggg atgaactgca ggcgcacggc
      421 cttgaaattg accagcagcg catcggggcc ggtgttgtac gggcgcgtcg gttgctcgtc
      481 gaagcgcgcg ggatcgaaca ggcccggcgc aaaatagctg cggtcaatca cgagatcgcc
      541 ttgaatctcg cgcaatcccc ggccgcgcag ttctctcagc agcagccaga agttctccag
      601 cgtgagcttg gggtcgcccc ggcccttgat caccaggtcg cccgttagca cgccgtcctt
      661 caatgtgccc gcggcgtagg cctcggtggt ccacgaaaag gcgggcccca gcagatcgag
      721 cgcggcgtag gtcgtcagca gcttgatggt ggacgcgggg ttcatcgcgc ggtcggcgcc
      781 cgcggtgaaa agggggcgct cggagccgat ctcgtggatg tagagcgccg ccgaggcctc
      841 cgggatgccg gccttcacga gcgcgcgggc taccggctcc ggcagttgcg cggatgcgcc
      901 gggcggcaac ccgagggcga acagcccgag tatcgccagg agcgagcgct ggatgggccc
      961 tgccctgtcc gcaacccagc tggttttgat ttctgtcatg cccggtccgg cattatgccg
     1021 cagcttcaac ccgcgggcag cgcatcgagg atgtctgcgg tgcgccgggc gagcagctcg
     1081 atttcttccg ggccgatcac gtagggcggc atgaagtaca cggtgttgcc gaggggccgc
     1141 agcaacagtt cgcgctcgag cgcggaggcg aagaagcggc gcgcaaaccc ggcgtcggcc
     1201 gtgtccacgt cgaaggcgag gatcatgccg gtcgagcgga aatgccggac gcggggatgc
     1261 tgcgcgatcg gctgtgcggc agccgtgatc cgcgcgctcc tttcccggtt cgcttcgatg
     1321 acgcgatctt cctcgaagat gtcgagggtg gcgagcgccg cccggcaggc gagcgcgttg
     1381 ccggtatagg aatgggaatg gaggaagccc cgcgccactt cgtcgtcgta aaacgcctgg
     1441 tagacggcat cgcgcgtcat cacgcaggag agcggcaggt agccgccggt gatgcccttg
     1501 gacagcagca gaaaatccgg agcgatggcc gcctgctggt gggcgaagag gctgccggtg
     1561 cggccgaagc cggtcatgat ttcgtcggcg atcaggtgca ctccataacg gtcgcagagc
     1621 gcccgcgccc gcttcaggta ttgcgcgtcg tgcattgcca tgccgctcgc cccctgcacc
     1681 agcggctcca cgatcagtgc cgcggtctcg ccgtggtgcc gctcgaggtg ccgttcgagc
     1741 gcctgcgcgc agcgctcggc gaagtcgcgc gccgactctc cctgctccgc caggcgccag
     1801 tcggggcttg gcacctggcg gcttgccatg aggagcggcg cataggtgtc cttgaagagg
     1861 gcgacatcgg tcaccgccag cgcgccgagc gtctccccgt gatagctgcc ggcgaggctg
     1921 acaaagccgg tcttggcggg ccggcccgca ttgcgccagt aatgaaagct catcttgagc
     1981 gcaatctcga ccgcggaggc gccgtcgctc gcgtagaagc aatggccgag cgccccgccg
     2041 gtggcgcgcg ccaggcgctc cgacagcgtc accgccggct cgtggctgca tccggcgagc
     2101 agcacgtgct cgagcttccc gagctggtcc gcgatggcgg cattgatgcg cggattgcag
     2161 tggccgaaca ggttgaccca ccacgagctg atggcgtcga gatagcgccg gccgtcgaaa
     2221 tcgtagagcc agcagccttc gccgcgcgcc accgggacga gcggcaacca ctcgtgctgc
     2281 ttcatctggg tacaggggtg ccagaccgcg gccaggctgc gctttagcag caaagcgttg
     2341 cgagtgctag tttgcatttt gagggcggag gctataaaaa atcaggagag cgaacggaaa
     2401 ccaggagacg gctttttcac tgggtcttcg cacaagcctt ggttataccc gatgggtgcc
     2461 ggcccgaagc ctgcgccccg cagcaacttc cccaggtcaa ccgcaggatt tcccgcagcg
     2521 ttgcattgca acgagtggca tcgttcttgc tcgtgccgca cttgtgcgtg gccgcagttt
     2581 gagcggcgcg tccagagacg aaagcagtgt agcacccact caaggagaga aacgtcatgc
     2641 agattcttga gctgatcgag gaccagatct cggcaaccgg cctgccgctg gtcggcatta
     2701 ccctcgcggc ggtgccttgc cccgacacgc cggtgatcct gacgctgcac tggcacggct
     2761 tcatcaagga gcggctggcc gacgccgagc aggccgcgcc ggtgtcgtac acgccggtgc
     2821 cgagttccgc cctgcaactg aacgaccgct ggcgcgacct cgtcgcggtg gactgggccg
     2881 cgatggaagc ggcgtgggaa ctgggcgcgt gggacgttgc gcgcgccgaa cgcaagggct
     2941 gcctgcggcc cggcgcgccc tccaccgaag cgctcgaatg cctgcaggcg ttcggcgcgt
     3001 tcccgtacgg catcaacggc aatcaggtgg tcgtgagcga tgcgcccgac gccgatgagc
     3061 tggtgcaact ggcagccgcc cgcggctacc tcatgtggct gttccggccg gtgagcggcg
     3121 gcatctgggc cgaggttgcc gatgacgcca ccctgacgcc caagggccgg cggccttccc
     3181 cgtgtccgca tcgcccgatt ccgcccgcct gtgacggcaa ccgcaagacg atctaccgct
     3241 tcggggtgcc cgccagcgct tcgggagacc gacggtaaca gtttattttc aagtagttag
     3301 tcactaacca ccctctggtt agtgagtgcc aacaccggtg cattgcctgt ggggttgtac
     3361 agctaaccta ttgtcttagt acggtcttat ctgtattatt agcactcgct ggagatgagt
     3421 gctaatcagg ccgttctccg tcgtttcagt accccagaca attcacagga ggcttagcaa
     3481 tgaaaattcg tcctctacac gaccgcgtga tcgtcaagcg gttggaagaa gagcgcaaga
     3541 ccgcatccgg gatcgtcatt cccgacaccg ccgccgagaa acccgaccag ggcgaagtca
     3601 tcgccgtggg caagggcaag atcctcgaag acggcaaagt gcgtcccctc gacatcaagg
     3661 tgggcgacaa gatcctgttc ggcaagtact ccgggcagac cgtgaaggtc aaaggcgatg
     3721 aactgctcgt gatgcgcgaa gaagacatca tgggcgtgat cgaagccaag taattccatt
     3781 ccgagaaaca ggagatcagt tatgtcagcg aaagacgtca aattccacga ctttgcccgc
     3841 agcaagatcg tcgccggtgt caacatcctg gccgatgcgg tgaaagtcac cctgggcccg
     3901 aagggccgca acgtggtgct ggaacgctcg ttcggcgccc ccaccgtcac caaggacggg
     3961 gtctcggtcg cgaaagagat cgagttgaag gacaagttcg agaacatggg cgcgcagatg
     4021 ctgaaggaag tcgcgtccaa gacctccgac gtggccggtg acggcaccac cacggcgacg
     4081 gtactggcgc aggcgatcgt gcaggaaggc atgaagtacg tggccgccgg catgaacccg
     4141 atggatctga agcgcggcat cgacaaggcg gtggaagcca tcgtcgccga gctgaagaag
     4201 atttccaagc cgtgcacgac cagcaaggaa atcgcccagg tcggcgccat ctccgccaac
     4261 gcggacctcg agatcggcaa gatcatctcg gacgcgatgg agaaggtcgg caaggaaggc
     4321 gtgatcacgg tcgaggacgg caagtcgctc aacaacgagc tggaagtggt cgagggcatg
     4381 cagttcgacc gcggttacct ctcgccgtac ttcatcaaca gcgccgagaa gcagatgtcg
     4441 atgctggaga atccgtacgt gctgctctac gacaagaaga tttccggcat tcgggaactc
     4501 ctgccggtgc tcgagcaggt cgcgaaggcg ggcaggccgc tgcttatcat cgccgaggaa
     4561 gtcgaaggcg aggcgctcgc cacgctggtg gtgaacaaca tccgcggcat cctgaagacc
     4621 tgcgcggtga aggcgccggg cttcggcgac cgccgcaagg ccatgctgga agacatcgcc
     4681 atcctgaccg gcggcgtcgt gatctcggaa gagaccggca tgcagctgga gaagatcacc
     4741 ctgaaggaac tcgggcaggc gaagcgcatc gagatcggca aggaaaacac cacgatcatc
     4801 gacggcaacg gcgacaccaa gacgatcgaa ggccgggtca agagcatccg cgcccagatc
     4861 gaggaagcga ccagcgacta cgacaaggag aagctccagg aacgcgtggc gaagctcgcc
     4921 ggcggcgtgg cgctgatcaa ggtcggtgcc gcaaccgaag tggagatgaa ggagaagaag
     4981 gcgcgcgtgg aagatgcatt gcacgcgacc cgcgctgcgg tggaagaggg cgtcgtcccg
     5041 ggtggcggcg tcgcttacct gcgcgcccgc gtcaacgcca agccgaaggc cgagaaccac
     5101 gaccaggaag cgggcatcaa gatcgtgatg cgcgctctgg aagagccgct gcgctggatc
     5161 accgccaacg ccggcgacga gccgtcggtg gtcgtcaaca aggtggtcga gggcaagggc
     5221 aatttcggct acaacgcgca gagcggccag tacggcgaca tggtggagat gggcgtgctc
     5281 gatcccacca aggtcacgcg ctacgcgctg cagaacgcgg cttcggtcgc ggcgctgatc
     5341 cttaccaccg atgcgatggt ggcggaactg ccgaaggatg aatcccacgc cggccacggc
     5401 cacggtggca tgggcggaat gggtggaatg gatatgtgac gagcccgtca gggcgagtca
     5461 tccctgcgca ggcggggatc cagtcgcaag ggaaaacccc gcttcggcgg ggttttcttt
     5521 ttgttgtggt tacctcagag gactgagggc cgcgtctgtc atccccgcga aggcggggat
     5581 ccagatcaac aagaagcccc gcgcaagcgg ggctttttct ttctcggatc gtagacgcag
     5641 tcacgaagaa tggcccgtgt cggacgaggc tattccctcg cttccgcgta ttgagcggca
     5701 agcgttgctt gtccgctcga attcgcgagt tggaacggcc gacaggtccg tttccacccg
     5761 agctgagcgg cgcgctcatg cgtccgctcg ggcgatattt cttgacgctc agtgtgttta
     5821 acgcttaaat agcgcgcttt ccggcgggac agggcagtcg ttcttgctgg ataatcgagc
     5881 ctcttcagca agttctgcag cacgtggccg ggtagctcag ttggtagagc agaggactga
     5941 aaatccttgt gtcggcggtt caattccgtc cccggccacc attcttttcc agtttcaagt
     6001 tacaagatgc aaggtacggg gaaaaacttg aaccttgaat cttgaacctt gtaactgtct
     6061 tatttcccgc cgtccgcctt caggatttcc attcccgcct gcaggcagcg ctcgatgcgc
     6121 gctttttcct tcgcgatcgc ctcgtccgtc cactgccaga agccgtgctt ggtcttcatg
     6181 ccgagatgtc ccttgtccac catgtcctgg acgaacggcg ggtaggcggt ctgggagtag
     6241 aggtgcgggt agagcgcggc gccgacgatg gcgttggtgt cccagcccga catttccttc
     6301 tgcagcatcg ggccgcaggc gatgaagcgg aagccgaagc cgtagcgcac ggcatcatcg
     6361 atgccttccg gcgaaatgat gccgtcgccg atcaggtaga gcgcctcgcg catcatcgca
     6421 tgctgcaggc ggttgcctac gaagccgggc acgtccttct tcacccagat cggcgccttg
     6481 cccaggtctt tcatgagact catcaggccc tcggcgactt tcgggtccgt ggcatccgcg
     6541 ctgacaatct cgaccagcgg caccaggtgc gccggcatga agaaatgcag ccccgccacg
     6601 cgcccgcgtc gcttgagtcc cttgccgact tcgccgatgg gaaaattcga ggtgttgctc
     6661 gtgagcgcga tgtcgggccg cgccagcgcc tcgatctgcc cgaacagttt ctgcttgagc
     6721 gccaggttct cgaccgccgc ctcgaccacg agcccgatgt ccttccacgg cagcgcctcg
     6781 agctcggcac aggttttcac gctggccgcg cgatcctgcg gcgcccccag ctttttcaat
     6841 ccggcggcga tgcgcgcagg cagcgcgtcg cgtgttttct gcgacgggct catcacgtgc
     6901 acgtcccagc cgccggcggc gaagatgatg gcgatgtccc cgcccatgat gccgccgccg
     6961 atgatggcga ccgcggaagg ctgcttcggc atgacgattt cctctgatta gtccggtaaa
     7021 aggcgcgcgt ttaacggcgg aactccgtcc cgctccaacg cgcgagcggc ggaattcccc
     7081 tagatcccgc tcgatgtttt cagcgcggcc gcgccgggtt catttagcac gcgtggcggc
     7141 ccacttcacc tcggccccgg tgtcggtcag cttgaacacg ccctggatgg tattgccgga
     7201 tacgcgtccg gtgtagtcgc gccggatggt gccgctggtg ccggcgagtt catggatcga
     7261 gaaggaaatc tggtcgccgg caagcttcac gttgcgcaac tgccccatcc tgccgttgtc
     7321 gagcctgacg agcccttcga cctgctggaa ctgctggcgc agcacgagct cgtattcctt
     7381 ctggccgctg ccggccacgc tccagcgcca ggtgccttcc acctgtgccg gcacgatcca
     7441 caggtaggcg gtgcgcccct cgacattgtc ggtctggtcg ggctgccagt cgtccatgct
     7501 gaaggcgtgc gagacgacgc gcgtgcccgg tttcatcttg aggatggtcg ggcggagctt
     7561 gaggttcaag ctcggcagga ggtagagcgt gaggacgttg gcgtggctga aatcggtctg
     7621 gaagatgtca ccattgatga acttcgcccg gtcggcgacg ccttccttgg cggccgcgcg
     7681 ttccgagagc ttgaccatgt cggggttgaa ctcgacgccc acggcctgcg cgccgcgctt
     7741 ggccgcggta atcacggtgc ggccgtcgcc gctgccgagg tcgtagtgga tgtcctgcgg
     7801 cgtcagcttc gccatgtcga gcatgcgctc gaccagcgcc tgcggcgtcg gcacccagat
     7861 gacgtccttg ccttcctggc cgacggacgg cttgtactcc tggccctgcg ccggcgcggt
     7921 tcccgccagg gcgagggcca gcgcggcgaa cagtgcggca aaaaaacggg tggtgcgcaa
     7981 tctcgagctg tgcatgggac tctccgtggt gaatggcgaa caaacctgcg ggtatggcgt
     8041 taaacgtttt gcgggcgcga tagtttacag caggcgaccg atgattattc ctgaggttcc
     8101 aggacgcgag ggcgggagag cgggagagcg ggagagcgca atgcgcgtgc gaattcccgg
     8161 tcttacccat ttcccgattt cccgctctcg cgatttcccg gcttcttgag gcggccatct
     8221 atcgtgacgc cgccggcgat catcgcgtcg atgtcggcgt ccgtgtagcc cacctcgcgc
     8281 aggatctcga cgctgttctg gccgagcttg ggcgccgcgc tcc
//
LOCUS       DBNGSG010000438         7515 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_483406, whole genome shotgun sequence.
ACCESSION   DBNGSG010000438 DBNGSG010000000
VERSION     DBNGSG010000438.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7515)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7515)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7515
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_483406"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /gene="hemF"
                     /locus_tag="AC158Q_11385"
     CDS             <1..741
                     /gene="hemF"
                     /locus_tag="AC158Q_11385"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HIR3340419.1"
                     /translation="ERAGVNFSHVHGASLPPSASASRPELAGRSFQALGVSLVLHPRN
                     PHVPTVHMNVRCFLAEKPGADPVWWFGGGADLTPYYPYAEDAQHFHRTCRDALAPFGA
                     DRYARYKKWCDDYFYLKHRAEPRGVGGIFFDDLNEPDFATCLALVKRVGENFLPAYLP
                     ILERRRAMAYGERERAFQAYRRGRYVEFNLVYDRGTLFGLQSGGRTESILMSLPPRAE
                     WRYDWRPEPGSPEEKLYTEFLIPRNWAD"
     gene            complement(855..2798)
                     /locus_tag="AC158Q_11390"
     CDS             complement(855..2798)
                     /locus_tag="AC158Q_11390"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3340420.1"
                     /translation="MDNTPSNIGKYQIQGVLGQGGMGIVYRAFDPAIHRPVAVKTITK
                     SALDPSDLQYALTRFRHEAQAVGRLTHPRITAIYDYGEDAEIAYIVMELVNGQSLYHH
                     IQNQAKFGLKEIGEMIRQLLDGLGYAHELGVVHRDIKPSNILINDDGRLKLTDFGIAR
                     LDSSTLTQVGEIMGSPGYMSPEQFLGTDIDARSDIYSVGVIAYELLANRRPFTGNNAE
                     VMRQVLNERPVNPSEHNPKISVQLDWAAQKALAKKQADRFQTAKEFSEAFVKGIDASI
                     RQGQRAPAASTLADEPTQRIDPKLIQAARMLAGLQPARADVPTQPNLPAPAPMPAAVH
                     AAAERPAAQETAAAGARKARILFVDDEERILTALRSVFRMNYHVFTATNGQEALEFVK
                     KFQPHVVVSDQRMPEMTGVELLRRVKETAPATVRLLLTGYSDLASIVGSVNEGEVFRF
                     VSKPWDNQEIQKTIGEAAAIALDLAEIAATPPIVPERMEAGVLVIGSGHETFNAVKRF
                     VGGACPVHHARDFDAAIGQLEANETALIIADIASRGGESLAAFKVLKHERPEILVIVL
                     TEASDSELVIELINQAQIFRFINKPVKPELLKQHTQAALTRYQSFKQNPHLTRQHQVA
                     ATAAGSSAGQKILERVKSLRNWF"
     gene            complement(2820..4661)
                     /locus_tag="AC158Q_11395"
     CDS             complement(2820..4661)
                     /locus_tag="AC158Q_11395"
                     /inference="COORDINATES: protein motif:HMM:NF039312.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3340421.1"
                     /translation="MPEPTANLSGLQRLLALRIARQALITAACILLVAILGFLYLKTQ
                     GADIKRGNEVLAALRGLKEIDARWDVDTWRLRFELGQPQPPVADLGLTLARIQKELAA
                     AAQSLGSPVLARSLPDLLRAFSQKAEMVEKFRKASVVTKQMLVSVLASDVEVAGLVRG
                     SWQDFRDRERLVAAESAAVQAIAEAQRYYFAPNEAQRKLVESVISDLRQAAGRLPPAL
                     RDGIQRLDERVQQLLSARSAEQEQFAKLSFLTAGPRVDSLTNAFSRELESALTDQEFY
                     RAYLVAYSGALLILLGYLATRLLASYRLLNKANEELEQRVAERTRELSETMRQLKDSE
                     AQLIQTEKMSSLGQMVAGVAHEINTPLAYVKNSLGSVTGKLPALTQLAAESEKLLDLL
                     RSGAANPHQLAQQFALTEQLIGQFKEHHVLEELQTLLKDGLYGINQISEIVVNLKNFS
                     RLDRSKMASFNLNEGLESTLLLAKHELKGHTVKKNFGDIPPVTCSPSQMNQVFLNLVN
                     NAAQAIESGRGVITLTTRRHNGEHVAVEVQDNGKGIPTEDLPKIFDPFFTTKDVGKGT
                     GLGLSIVYKIIQSHGGSISVDSAVGVGTRFTVVLPLTPPEPVEEEAA"
     gene            complement(4667..5371)
                     /locus_tag="AC158Q_11400"
     CDS             complement(4667..5371)
                     /locus_tag="AC158Q_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3340422.1"
                     /translation="MLEVRGLEVRYGGINAVKGIDLEVRAGELVTLIGANGAGKTTTL
                     KALAGLLKPAAGRIHYNGGDITARPAHELVRQGLALVPEGRGVFGRLTVEENLAMGAY
                     SRRDAARIKADFDRVYERLPRLAERRRQLAGTLSGGEQQMLAIGRALMGRPRLLLLDE
                     PSMGLAPLMVQKIFETIRAIAAEGTTLLLVEQNARLALETCHRGYVMESGSITLADGA
                     QRLLANPQVRQAYLGE"
     gene            complement(5364..6143)
                     /locus_tag="AC158Q_11405"
     CDS             complement(5364..6143)
                     /locus_tag="AC158Q_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3340423.1"
                     /translation="MSPAEAPLLTARSISKRFGGLAALAEISLTVRRHEIFGLIGPNG
                     AGKTTLFNVLTGLYRPDAGEFTFDGARLTGVKPHRVAECGIARTFQNVRLFANMTALE
                     NVMVGRHARTRAGVLGAVLRGASTRAEEAEIAGRAFALLEYVGIASRAAEPAHSLSYG
                     DQRRLEIARALATDPRLLALDEPAAGMNATERAELTRLLERIRGDGLTLLLIEHDVKL
                     VMGLCDRVAVLDYGRLIAEGAPAEVRRDPAVIAAYLGGDLA"
     gene            complement(6130..7239)
                     /locus_tag="AC158Q_11410"
     CDS             complement(6130..7239)
                     /locus_tag="AC158Q_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR3340424.1"
                     /translation="MDALWRRLQRDPRAAWTAYAAVAVILIALPFAIDAVFGRAWVRI
                     IDVALLFILLGLGLNIVVGYAGLLDLGYIAFFAVGAYSYALLASPQFGIHGSFLVLLP
                     FAAFVAAVFGVLLGAPTLRLRGDYLAIVTLGFGEIIRIFLNNLNRPFNITNGPQGITL
                     IDPLAVGGVALNQTYTIAGLTLAPVHSYYFVFLLCASATIFISLRLEDSRIGRAWIAI
                     REDELAAAAMGINTRNLKLLAFAMGATFGGVAGGLFASFQGFISPESFTLLDSIMVLC
                     MVVLGGMGNVGGVVLGAVLLTALPEALRYAAPLQKDWLGDVYVDPSDLRMLLFGLALV
                     LMMLFRPAGLWPSSIRRRELVEASADAPEQIHVAR"
     gene            complement(7248..>7515)
                     /locus_tag="AC158Q_11415"
     CDS             complement(7248..>7515)
                     /locus_tag="AC158Q_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006396016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR3340425.1"
                     /translation="IGHYFMGFMLGLKAFTAAVLGGIGNVAGAVLGGLLLGIIESLAA
                     GYIGDLTGGVLGSNYRDIFAFLVLIGVLVLRPSGILGQSNGERA"
BASE COUNT         1243 a         2453 c         2508 g         1311 t
ORIGIN      
        1 gagcgcgcgg gcgtgaactt ctcgcatgtg catggcgcga gcctgccgcc gtcggccagc
       61 gccagccggc cggaactcgc gggccgctcg ttccaggcgc tgggcgtctc gctggtgctg
      121 cacccgcgca atccccacgt tcccaccgtg cacatgaacg tgcgctgctt cctggcggag
      181 aagcccggcg ccgatcccgt ctggtggttc ggcggtggcg cggacctgac gccctattat
      241 ccgtacgccg aggacgcgca gcatttccac cgcacctgcc gcgacgcgct cgccccattc
      301 ggggcagacc gctacgcccg ctacaagaaa tggtgtgacg actattttta cctcaagcac
      361 cgtgcggagc cgcgcggggt gggcggcatc ttcttcgacg atctcaacga gcccgatttc
      421 gcgacctgcc tcgcattggt gaagcgcgtg ggcgagaact ttctgccggc ctatcttccc
      481 atcctcgagc gtcgcagggc gatggcctac ggggagcgcg aacgcgcatt ccaggcctat
      541 cgccgcggcc gctacgtcga gttcaatctc gtgtacgacc gcggcacgct gttcggactg
      601 cagtcgggcg ggcgcaccga gtccatcctg atgtcactgc cgccgcgggc cgagtggcgt
      661 tacgactgga gacccgaacc gggcagcccg gaagaaaagc tttacaccga attcctgatt
      721 ccgagaaact gggcggactg agatcagtgt tgagttttaa atgtgcggtt ttgagttaac
      781 ttcaactcat cactcatcac tcgacactcg acactcgaca ctcatcactc gcccctcggc
      841 gccggccggc gcggtcagaa ccagttcctc agcgatttca cgcgttcgag tatcttttgg
      901 ccggcggaac tcccggcagc cgtcgcggcg acctggtgct ggcgggtgag gtggggattc
      961 tgcttgaacg actggtagcg ggtgagcgcc gcctgggtgt gctgtttcag caactccggc
     1021 ttcaccggct tgttgatgaa acggaaaatc tgcgcctggt tgatgagttc gatgacgagt
     1081 tccgagtcgg atgcctccgt caacacgatg accagtatct cggggcgttc gtgtttcagg
     1141 accttgaagg cggcgagcga ttctccgccc ctgcttgcga tgtcggcgat aatcaacgcg
     1201 gtctcgttcg cctccagttg tccgatcgcg gcgtcgaaat cccgggcgtg gtgcacgggg
     1261 caggcgccgc ccacgaatcg tttcacggcg ttgaacgtct cgtggcccga gccgatcacc
     1321 agcacccccg cctccatcct ctcgggaacg atgggcggtg tcgcggcaat ctcggcaaga
     1381 tccagcgcaa tcgccgctgc ctcgccaatg gtcttctgga tttcctggtt gtcccagggc
     1441 ttggagacga agcggaacac ttcgccttcg ttcaccgagc cgacgatgga cgcgaggtcc
     1501 gagtagccgg tcagcagcag ccttaccgtc gccggcgcgg tttccttcac ccgccgcagc
     1561 agctccacgc ccgtcatttc cggcatgcgc tggtcgctca ccacgacgtg gggctggaat
     1621 ttcttgacga actcgagcgc ttcctggccg ttggtcgcgg taaacacgtg gtagttcatg
     1681 cggaagaccg agcgcagcgc cgtgaggatg cgctcctcgt cgtcgacgaa caggatgcgc
     1741 gccttcctgg caccggcggc cgcggtttcc tgggccgccg gccgctcggc ggcggcgtgc
     1801 acagcagcag gcatcggtgc aggcgccggc aggttgggct gggtaggcac atcggcccgc
     1861 gcgggttgca ggccggctag catgcgcgcg gcctggatca gcttggggtc gatacgctga
     1921 gtcggttcgt ccgcaagggt ggacgctgcg ggcgcgcgct ggccctggcg gatgctggca
     1981 tcgatgcctt tgacaaaagc ctcggagaac tctttcgcgg tctggaaacg gtccgcctgt
     2041 ttcttggcca gcgctttctg cgcggcccag tcgagctgca ccgagatttt cgggttgtgc
     2101 tcagacggat tgaccgggcg ctcgttcagc acctggcgca tcacctcggc gttgttgccg
     2161 gtgaacgggc ggcggttggc gagcagctcg taggcgatca cgccgaccga atagatatcg
     2221 ctgcgcgcgt cgatgtcggt gccgaggaac tgctccggcg acatgtagcc cggcgagccc
     2281 atgatctcgc ccacctgggt gagggtggag gaatcgaggc gcgcgatgcc gaaatcggtg
     2341 agcttcagcc gcccgtcgtc gttgatcagg atgttggacg gcttgatatc gcggtgaacg
     2401 acgcccagct catgagcgta gcccaggccg tcgagcagct ggcggatcat ctcgccgatt
     2461 tccttcagcc cgaatttcgc ctggttctgg atgtgatggt agagcgactg gccgttgacc
     2521 agctccatca cgatgtaggc gatctcggcg tcctcgccat agtcatagat cgcggtgatg
     2581 cgcgggtgcg tgagccttcc gacggcctgc gcttcgtgcc ggaagcgcgt caacgcatat
     2641 tgcaggtccg acgggtcgag cgccgacttg gtgatggtct tgaccgccac cgggcggtga
     2701 atggcgggat cgaatgcgcg gtagacgatg cccatgccgc cctgcccgag cacgccctgg
     2761 atctggtact tgccgatgtt ggacggggtg ttgtccatgg gggcggtgaa gggccgtgct
     2821 caggccgctt cttcttcaac cggttcgggg ggggtcaacg gtaacaccac cgtgaaccgg
     2881 gtgccaactc cgacggcgga gtccacgctg atgctgccgc cgtggctctg gatgatcttg
     2941 tagacgatcg agagcccgag cccggtgccc ttgcccacgt ccttggttgt gaagaaggga
     3001 tcgaagatct tggggaggtc ttccgtcggt atgcccttgc cgttgtcctg cacctccacc
     3061 gcgacgtgct ccccgttgtg gcggcgcgtg gtgagcgtga ttacgccccg cccggactcg
     3121 atcgcctgcg ccgcattgtt gacgaggttc aggaatacct ggttcatctg cgagggcgag
     3181 caggtaaccg gggggatgtc gccgaaattc ttcttcacgg tatggccctt cagctcgtgc
     3241 ttggcgagca gcagcgtgct ctccagcccc tcgttcaggt tgaagctggc catcttgctg
     3301 cggtcgagcc gggagaagtt cttgaggttg accacgatct cggagatctg gttgatgccg
     3361 tagagcccgt ccttcaacag ggtttgcagc tcctccagca cgtggtgttc cttgaactgc
     3421 ccgatcagct gctccgtcag cgcgaactgc tgcgcgagct ggtgggggtt ggcggcgccg
     3481 gagcgcagca ggtccagcaa cttctcggat tcggcggcga gctgagtgag cgcgggcagc
     3541 tttcccgtta ccgagcccag gctgttcttg acgtaggcga gcggcgtgtt gatctcgtgg
     3601 gcgacgcccg cgaccatctg gccgagggag gacatcttct cggtctggat cagctgcgcc
     3661 tccgaatcct tcagttggcg catggtttcc gagagttccc gggtgcgctc cgcgacccgt
     3721 tgctccagct cctcgttggc cttgttgagc agccggtagc ttgccaggag ccgcgtcgcc
     3781 aggtagccca gcaaaatgag cagcgcgccg gagtaagcga cgagataggc gcggtagaac
     3841 tcctggtcgg tgagcgcgct ctccagttcc cgcgaaaacg cgttggtgag gctatcgacc
     3901 ctcggtccgg ctgtgaggaa cgagagtttt gcgaactgct cctgctcggc agacctggcg
     3961 gagagcagct gttgcacccg ttcgtcgagg cgctggatgc catcgcgcag cgccgggggc
     4021 agccggcccg ccgcctggcg caggtcagag atgaccgatt cgacgagctt gcgctgcgcc
     4081 tcgttcgggg caaagtaata ccgctgcgcc tcggcgatcg cctgcaccgc tgcgctctcc
     4141 gccgccacca gccgttcccg gtcgcggaag tcctgccagg agccccgcac caggcccgca
     4201 acctcgacat cggacgccaa aacactcacg agcatttgct tggtgaccac gctggccttc
     4261 ctgaactttt ccaccatctc cgccttctgg gagaacgcgc gaagcagatc cggcaggctg
     4321 cgcgcgagca ccgggctgcc gagcgactgg gcggcggcgg ccagttcctt ctggatacgc
     4381 gccagcgtga ggccgaggtc cgcaaccggc ggctgcggct ggccgagctc gaagcgcagg
     4441 cgccaggtat ccacatccca gcgcgcgtcg atttccttca gcccgcgcag cgccgccagc
     4501 acctcgttgc cgcgcttgat atcggcgccc tgggtcttga ggtagagaaa gcccaggatg
     4561 gctaccagca gaatgcaggc cgcggtgatg agcgcctggc gagcaatccg cagcgccagc
     4621 aggcgctgca ggccggacag gttggcggtc ggctcgggca tcgcgctcac tcgcccaggt
     4681 aagcctgccg tacctgggga ttggcgagca ggcgctgcgc gccgtcagcc agcgtgatgg
     4741 acccgctctc catcacgtaa ccgcgatggc aggtctcgag cgcaagccgg gcgttctgct
     4801 ccaccagcag gagcgtcgtg ccctcggccg cgattgcgcg tatggtttcg aagatcttct
     4861 gcaccatcag tggcgcgagc cccatgctgg gttcgtcgag cagcaacaac cgcggccggc
     4921 ccatcagcgc ccgcccgatc gccagcattt gttgctcgcc tcccgaaagg gtaccggcga
     4981 gctgccggcg ccgctcggcc agccgtggca ggcgctcgta tacgcggtcg aaatcggctt
     5041 tgatgcgcgc cgcatcgcgc cggctgtagg cccccatcgc gaggttctcc tccacggtga
     5101 gccgcccgaa cacgccgcgc ccctcgggca ccagcgccag cccctgtcga accagctcgt
     5161 gggccgggcg cgctgttatg tcgccgccat tatagtggat gcggccggcc gccggcttca
     5221 acaggccggc aagcgccttc agcgtggtcg tcttgccggc gccgttggcc ccgatcagcg
     5281 taacgagttc gcctgcgcgc acctcgagat cgatgccctt gacggcgttg atgccgccgt
     5341 agcgaacctc gagccccctc acttcaagca aggtcgcctc caagataggc ggcgatgacg
     5401 gcgggatcgc ggcgcacttc cgccggcgcg ccttcggcga tcaggcggcc gtagtcgagc
     5461 accgccacgc ggtcgcacaa ccccatcacg agcttaacgt cgtgctcgat caggagcagc
     5521 gtcaggccat cgccgcgaat tctctcgagc agccgcgtga gttcggcgcg ctcggtggcg
     5581 ttcatcccgg cggcgggctc gtcgagcgcg agcagcctgg ggtccgtcgc cagcgcgcgg
     5641 gcgatctcca ggcggcgctg gtcgccgtag gagaggctat gggcgggctc cgcggcgcgg
     5701 cttgcgattc cgacgtactc gagcagcgcg aaggcgcggc cggcgatctc cgcttcctcg
     5761 gcccgggtcg aggcgccacg cagcaccgcg cccagcacgc cggctcgcgt gcgcgcatgg
     5821 cgccccacca tcacgttctc gagcgcggtc atgttcgcga acagacggac gttctgaaag
     5881 gtgcgggcga tgccgcattc cgcgacccgg tgcggcttga cgccggtcag gcgcgccccg
     5941 tcgaaagtga actcgccggc gtcgggccgg tagaggccgg tcagcacgtt gaagagcgtg
     6001 gtcttgcccg cgccgttggg cccgatcaat ccgaagattt catgacgcct gaccgtgagc
     6061 gagatctccg cgagggcggc gagccccccg aagcgcttgc tgatcgagcg ggcggtaagc
     6121 agcggcgctt cagcgggcga catggatttg ctccggcgcg tcggcggacg cctcgaccag
     6181 ttcgcggcgg cggatgctcg atggccacag gcccgccggg cgaaacagca tcatcagcac
     6241 cagcgccagg ccgaacagca acatgcgcag gtccgacgga tcgacgtaga cgtcgcccag
     6301 ccagtctttc tgcagtggcg cggcgtagcg cagggcttcg ggcagggcgg tgaggagaac
     6361 cgcgcccagc accacgccgc cgacgtttcc catgcccccc agcactacca tgcacagcac
     6421 catgatggaa tcgagcagcg tgaagctctc ggggctgatg aagccctgga acgacgcgaa
     6481 caagccgccc gcgacaccgc caaaggtcgc gcccatggcg aaagccagca acttcagatt
     6541 gcgcgtgttg atgcccatcg cggccgccgc cagttcgtcc tcgcggatcg cgatccacgc
     6601 gcggccgatg cgcgaatcct ccagccgcag cgagatgaag atggtggccg aagcgcaaag
     6661 gagaaacacg aagtaatagc tgtgcaccgg cgccagggtg agcccggcga tcgtgtaggt
     6721 ctggttgagc gccacgccgc cgacagcgag cgggtcgatc agcgtgatgc cctgcggccc
     6781 gttggtgatg ttgaacggac ggttaaggtt gttgagaaaa atccggataa tttcgccgaa
     6841 gcccaacgtg acgatcgcga gataatcgcc gcgcaaccgc agagtcggtg cgccgagcag
     6901 cacgccgaac acggcagcga cgaaggcggc gaagggcagc agcacgagga atgacccatg
     6961 tatgccgaac tggggggagg ccagcagcgc gtagctgtag gcgccgaccg cgaagaacgc
     7021 gatgtagccc aggtcaagca ggcccgcgta gccgaccacg atgttcaatc ccagcccgag
     7081 cagtatgaac agcagcgcta catcgatgat gcgcacccag gcgcggccga acaccgcgtc
     7141 gattgcgaac ggcagcgcga tcaatatgac ggcgacggcg gcgtaggctg tccaggcggc
     7201 gcgcggatcg cgctgcagcc gccgccacag ggcgtccatg gcggtcatca ggcgcgctcc
     7261 ccgttcgact ggcccaggat tccggacggc cgcaacacca gcacgccgat cagcaccagg
     7321 aaggcgaaga tgtcgcgata attgctgccc aggaccccgc cggtgagatc gccgatgtag
     7381 cccgcggcga ggctttcaat gatcccgagc aggagcccgc ccagcacggc gcccgcgacg
     7441 ttgccgatgc cgcccagcac cgcggcggtg aaggccttca gccccagcat gaagcccatg
     7501 aagtagtgac cgatg
//
LOCUS       DBNGSG010000439         1631 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3539863, whole genome shotgun sequence.
ACCESSION   DBNGSG010000439 DBNGSG010000000
VERSION     DBNGSG010000439.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3539863"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="AC158Q_11420"
     CDS             complement(<1..641)
                     /locus_tag="AC158Q_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR3340426.1"
                     /translation="MDMFANLIYGFSVALSLQNLIYCFVGVFVGTAIGVLPGVGPLVT
                     IAMLLPITFNLPPIPALIMLSGIYYGSQYGGSTTSILMNLPGETASVVTCIDGYQMAK
                     QGRAGPALAIAAVASFFAGCVGTLLIALFGPPIAEMALKFGAPEYFSLMLMALIAAAC
                     LSSGDMVKALAMVVLGLLLGIIGSAVNSGMARYSFGIPELTDGMNFTVVAVGL"
     gene            complement(646..1098)
                     /locus_tag="AC158Q_11425"
     CDS             complement(646..1098)
                     /locus_tag="AC158Q_11425"
                     /inference="COORDINATES: protein motif:HMM:NF018979.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HIR3340427.1"
                     /translation="MKVKLYDNKDFLAGLLMIAIGAIGFYMALDYPFGSALRMGPGYF
                     PRVLAGIIIAFGVYVGLRGLRTGEKVEGIWGWKALAFVTAAFVAFGWLMDHVGMIPSL
                     VVLFFVSALAGHEFKWKEVVILAAVMIVFAWAVFIYGLGLPYRLFWWD"
BASE COUNT          344 a          551 c          480 g          256 t
ORIGIN      
        1 aacaggccca ccgccaccac cgtgaaattc atgccgtcgg tcagctccgg gatgccgaag
       61 ctgtagcgcg ccatgccgga attcacggcg ctgccgatga tacccagcag cagccccagc
      121 accaccatgg cgagcgcctt gaccatgtcg ccgctcgaga ggcaggcggc ggcaatgagc
      181 gccatcagca tgagcgaaaa gtactcgggc gccccgaact tgagcgccat ttccgcgatc
      241 ggcgggccga acagggcgat cagcagcgtg cccacgcagc cggcgaagaa cgaggcgacc
      301 gccgcgatcg ccagcgccgg cccggcgcgc ccctgcttcg ccatctggta gccatcgatg
      361 cacgtcacga ccgaggccgt ctcgcccggc aggttcatca atatggacgt ggtggagccg
      421 ccgtactgcg agccgtaata gatgcccgac agcatgatca gcgccggtat cggcggcaga
      481 ttgaaggtga tcggcagcag catggcgatc gtgaccagcg gcccgacgcc gggcagcacg
      541 ccgatcgccg tgccgacgaa tacgccgacg aagcaataga tcaggttctg cagcgacagc
      601 gcgacgctga aaccgtagat caggttggca aacatgtcca taggattaat cccaccagaa
      661 gaggcgatac ggcaggccaa ggccgtaaat gaagacggcc cacgcaaaca cgatcatgac
      721 cgctgccagg atcacgactt ccttccactt gaactcatgg ccggccaacg cggacacgaa
      781 aaacagcacc accagcgacg ggatcatccc cacgtggtcc atcagccagc cgaatgccac
      841 gaacgccgcc gtgacgaacg ccagcgcttt ccagccccag atgccttcca ccttttctcc
      901 cgtgcgcagg ccgcgcaggc cgacatagac gccgaacgcg atgatgatgc ccgcgagcac
      961 ccgcgggaaa taccccgggc ccatgcgcag cgcgctcccg aagggataat cgagcgccat
     1021 ataaaagccg atggcgccga tagctatcat cagcaggccc gccaggaaat ccttgttatc
     1081 gtacaacttg accttcatct accctccttc tccaaaacgg cgggcgctct ggaaccgaac
     1141 cgcgcacccg gacacagtca gccccctgcg gtgcctgtcg gggcatgtac tgcggaaaag
     1201 cgtcgtctat cgccaggaga atgtttttcg gcgcattttt tgccccctgc cgtcctgcct
     1261 ccagcgggtt cgccaagctt ctgtcctacc ggcgccggcg ttggtccact atgtgcgatg
     1321 cggattctaa cggatttatt ccgcgttatc gaggggtatt ctcatcaaat ttcgggaata
     1381 atccaccccg atttccgtgt gctcgggaca gcaggtcggg gagctgccga ccctgaccaa
     1441 tcaggatggg accgcgcgag cgcgacggga tagaggatcc ccgggtgccc aataattgtg
     1501 caccctccgt tcccaccggc ggaccgggag cgccgcccag gccaacggga ttatgaacgt
     1561 tcgttcatct taccggtgcc cgtggccgat gcgaagcggg cggccagcgc cggccccgcc
     1621 tcagggcgcg c
//
LOCUS       DBNGSG010000440         2876 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3201551, whole genome shotgun sequence.
ACCESSION   DBNGSG010000440 DBNGSG010000000
VERSION     DBNGSG010000440.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3201551"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..1076)
                     /locus_tag="AC158Q_11430"
     CDS             complement(9..1076)
                     /locus_tag="AC158Q_11430"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HIR3340428.1"
                     /translation="MTPGKTKKTVFWFDDGKRPNVADCLDKEKDIVLHRLSYEGPKAD
                     NWGAMSASQVYCVTSTRQELPEDYRINAALLERCPDLLAVSTSGAGYDTVDVAACTAA
                     GVLVVNQTGGNADAVAEHTVAMMLSLTKNIPQTDRSVRCGQRAPRESFKGWNARGRTV
                     GVVGCGNVGSRVARICGLGLQMKVLACDPYLTAEQIRERGAAKVDLPTLLAESRFVSI
                     HCPYNDETRDLIGARELAAMQPGAILITTARGGIVNEEALAEALKSGHLGGAGVDVWI
                     EEPPPLSHPLLAFDNLIATYHTAGITHDSRNNLAEWNASQVAGILRGERPPRLINPDA
                     LERCGQRFERIFGFRPNFNGK"
     gene            1349..2743
                     /locus_tag="AC158Q_11435"
     CDS             1349..2743
                     /locus_tag="AC158Q_11435"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3340429.1"
                     /translation="MASLSRQLARWVVGLRYQDLPPEVADRAKGVTLHCLASVLLGSK
                     TGPGRRAVELIADEEAGVRKGATLMVHGGTATKGGAAYANSEMALAGGKFDTFRMLTH
                     PGTSIIPGALVAAEAAGAPGQDFVTGVAAGYEVMERLAADFIPTVMSRGFHPGPVFGI
                     FGPAVAAAKILGLDEEQVNSTSALCASLASGNVEGARSGGKALREGAAVRNALLAVAL
                     AQRGHVGGETVLEGEAGFYHSYAGNNQGRLAYSFTGDTRASLEAIAAGLGKEWLFLET
                     LYRIYFTAGYNIAHIDITAKLCEEHGIRHQDVERVEAVVNWLETQYPSPAFPSRREDG
                     AARPGSTAYFTAYGIVKRGYPVLRGQALDPADDPPEVLELMKRVTIIPSHRMTLFGPR
                     FTIHTKDGRSYTKQATGREFIWDFEEEARRIRGVIPGLPIPAAQFETMIATCRDLDRE
                     ERADRLVQLTLKGK"
BASE COUNT          448 a          971 c          974 g          483 t
ORIGIN      
        1 atatccggtc actttccatt gaagttcggt cgaaagccga agatgcgctc gaagcgctgc
       61 ccgcagcgtt ccagcgcatc cgggttgatg aggcgcggcg gacgctctcc gcgcaggatg
      121 ccggccacct gcgaggcgtt ccattcggcc aggttgttgc gcgaatcgtg cgtgatgccg
      181 gccgtgtgat aggtggcgat caggttgtcg aacgcgagca gcgggtgcga gagcggcggc
      241 ggctcctcga tccagacgtc cacccccgcg ccgccgaggt gcccggactt gagcgcttcc
      301 gcgagggctt cctcgttcac gatgccgccg cgcgccgtgg tgatgaggat ggcgccgggc
      361 tgcatcgcgg cgagctcgcg ggccccgatc aggtcgcggg tctcgtcgtt gtacgggcag
      421 tggatcgaga cgaagcgcga ctcggcaagc agcgtcggca ggtccacctt ggccgcgccg
      481 cgttcccgga tctgctccgc ggtgagatac ggatcgcacg cgagcacctt catctgcagc
      541 cccaggccgc agatgcgcgc cacgcggctg ccgacgttgc cgcatcccac cacgccgacc
      601 gtgcggcccc gggcgttcca gcccttgaag gactcgcgcg gggcgcgctg gccgcagcgc
      661 accgagcggt ccgtctgcgg gatgttcttg gtgagcgaga gcatcatcgc caccgtgtgc
      721 tccgccaccg cgtcggcatt gccgccggtc tggttcacca ccagcacgcc cgccgcggtg
      781 cacgccgcga cgtccaccgt gtcgtagccc gcgccgctgg tggaaacggc gaggagatcg
      841 gggcagcgct cgagcagcgc cgcgttgatg cggtagtcct cgggcagttc ctggcgcgtc
      901 gaagtgacgc agtagacctg cgaagccgac atggcgcccc agttgtccgc cttcggaccc
      961 tcgtaggaca ggcggtgcag gacgatgtct ttttccttgt cgaggcaatc ggcgacgttc
     1021 ggccgcttgc cgtcgtcgaa ccagaacacg gtttttttgg ttttgccggg cgtcatgagt
     1081 ttccgctgcg aaggggtaat cgaatcctac cgcagaaccg gtttctcgtt tctcgttgct
     1141 cgttactcgt ttctcgagtg tcgagtgtcg agtgtcgagt gtcgggtgtc gcggggtaga
     1201 gctctgtaac gttttgttca ctcgacactc cttccctcat ccctcatccc tcatccctca
     1261 tccccgctct tccttccgcc ttcaagagtg tcccgccacc cctgacttga gttatcttcg
     1321 gtggcatccc taatgctgga gtaacgccat ggcatctctc agccggcagc tcgcgcggtg
     1381 ggtcgtcggc ctgcgctacc aggaccttcc gcccgaggtg gccgatcgcg cgaagggcgt
     1441 caccctccac tgccttgcct ccgtgctgct gggctcgaaa accgggccgg gcaggcgggc
     1501 ggtcgagctc atcgccgatg aggaggccgg cgtgcgcaag ggcgcgaccc tcatggtcca
     1561 cggcggcacg gcgacgaaag gcggcgccgc ctacgccaac tcggagatgg cgctcgccgg
     1621 cggcaagttc gacaccttcc gcatgctcac ccaccccggc accagcatca ttccgggcgc
     1681 cctcgttgcg gcggaagcgg cgggcgcccc gggccaggat ttcgtgaccg gggtcgcggc
     1741 cggctacgaa gtcatggagc ggctggcagc cgacttcata ccgaccgtga tgtcgcgcgg
     1801 cttccatccc ggcccggtgt tcggcatctt cggccccgcc gtcgccgcgg cgaaaatcct
     1861 gggcctcgac gaggagcagg tgaacagcac gagcgcgctc tgcgcgagcc tcgcctccgg
     1921 caacgtcgag ggagcccgca gcggcggcaa ggccctgcgc gaaggcgccg cggtgaggaa
     1981 cgccctgctc gcggtcgcgc tcgcgcagcg gggccacgtc ggcggcgaga ccgtgctcga
     2041 gggcgaggcc ggcttctacc actcgtacgc gggcaacaac cagggccggc tcgcctacag
     2101 tttcaccggc gacacgcgcg cgagcctgga ggccatcgcg gcgggcctgg gaaaggaatg
     2161 gctgttcctc gagacgctct accgcatcta cttcaccgcc ggctacaaca tcgcgcacat
     2221 cgacatcacg gcgaaactgt gcgaagagca cggcatccgc caccaggacg tcgagcgcgt
     2281 cgaggccgtg gtgaactggc tggagacgca gtaccccagc ccggcgttcc cgagccggcg
     2341 cgaggacggc gcggcgcggc cgggaagcac cgcttacttc accgcgtacg gcatcgtgaa
     2401 gcgcggctat cccgtgctgc gcggccaggc gctcgatccc gccgatgacc cgccggaagt
     2461 gctggagctg atgaagcggg tgacgatcat cccgtcgcac cggatgaccc tgttcggccc
     2521 gcgcttcacg atccacacga aggacggcag gagctacacg aagcaggcga ccgggcgcga
     2581 gttcatctgg gatttcgagg aggaggcgcg ccgcatccgc ggagtcattc ccggcctgcc
     2641 gatcccggct gcgcagttcg agacgatgat cgcgacctgc cgcgacctcg atcgcgagga
     2701 acgggcggac cggctggttc agctcacgct gaagggaaag tgacgagggt cgaatgacga
     2761 gggtcgagtg tcgagtgtcg agggtcgagg gcgtgttgaa tacgacgcct gacctgcgac
     2821 gcccatctgc gacgcttcac ctgagacgcc tcatctgaga cgcttcatct gagacg
//
LOCUS       DBNGSG010000441         6296 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2861027, whole genome shotgun sequence.
ACCESSION   DBNGSG010000441 DBNGSG010000000
VERSION     DBNGSG010000441.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6296)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6296)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6296
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2861027"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1453
                     /locus_tag="AC158Q_11440"
     CDS             <1..1453
                     /locus_tag="AC158Q_11440"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR3340430.1"
                     /translation="TTTCGPGVSQLATALITASRAASPVVAFCGEYPNNDDEYNQRMD
                     QQSFAAGCETAFIRITSPDIADDCVRKAFYTAKLESRPVMLSAPMDVQQMAFEDDDEA
                     YKPSSTILPKRTIHPDPDEIARAADLIGKAKHPVIICGRGAMWAGAGPSVVKLADRIG
                     ALIGTSLMAKGWLAEQEWYAGISGTYGARNSLKLFEESDCVIAVGATMNRYTTEHGYL
                     YPNAKFVHLDVKPHLVMSGGRAADCYVQTDARAGVEALEAELAKRQVRMTGYRTSEVK
                     KLLAARNVDRAEFPIEPDHVDAREIIQTLDEMLPPAIGVVTGSGFTAGFSNMLFNKPR
                     SLVLPGHFFGCIGQMLPAAMGVVVASGNKPHVLCDGDASVMMHIGEFETMVRYKMPLM
                     VVCLNNQALGSEYYKLDAHKMKAELSVVETPDLGQVGKAFGGRGKLVRSVEDLKTATQ
                     EFLASPGPMLLDVRISRSVVTLPYRRIHYGRDE"
     gene            1534..2340
                     /locus_tag="AC158Q_11445"
     CDS             1534..2340
                     /locus_tag="AC158Q_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340431.1"
                     /translation="MNLLYRQRGFIQFLAQLVVALILLALAARFVWWVATETNFDQVA
                     TILWGLGFVAAYFITLGNYFVLRMPAGFGVFALIGLASLPLSWLAASRVALLVLLAAQ
                     LAFSFYAGRAISVRLRARHSEMEEDEAARKGAGLVTGLGRWAMLALAALMVLLIGPLL
                     VLALVSVAVDLSDQELRWLAALWGLAGVAWFGYRFGVVKWRRVPACAWIYLLATVPIL
                     VADALAGPFAEGAGVQVAYTTMPGALAAAFVEVFVFGGGQLDPDDRLPDS"
     gene            2421..3644
                     /locus_tag="AC158Q_11450"
     CDS             2421..3644
                     /locus_tag="AC158Q_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3340432.1"
                     /translation="MTTPSQAATTHNGFPPIVSLLLNVAHGIDHMFLLIFATAVASIA
                     VEFGYATWTDLMPYSVGAFALFGLGALPAGRLGDVWGRRSMMIVFFIGIGAAALLTAL
                     TQNAWQLAAALTLLGAFAAIYHPVGIPMLVQNAPNPGAVIGVNGLAGNLGIAVAALVT
                     GFLVKWIGWRAAFAIPGLIAIGCGIVFAIVCPKEEHAPSKGRGGKAKVVLTPAMLVRA
                     FAVMTAAAATSSILFNFTTNGNSQLLSESFRGVIEDPARLGALLAVIYTVASFAQIVV
                     GRLIDVMPFKPLQLWMSVAQVPLLIIAAYTQDWGMFVALMLVMIFIFGSIPFTDAMIV
                     RYVDDRLRSRVAGMRLTVAFGISSAAVWLLGPMVKSVGFATLLWIMAGIAAVKAAIVL
                     FLPDESSSTVGAART"
     gene            3756..4211
                     /locus_tag="AC158Q_11455"
     CDS             3756..4211
                     /locus_tag="AC158Q_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrophosphatase"
                     /protein_id="HIR3340433.1"
                     /translation="MKKSFAQRIAAMNAMYRLPAGDRPGLPADVADRLVKFKATLLDE
                     VHEIDEIVQLSKNGGAALDVAVAIADLLGDITVYCRSEALKYGLPLEEVLDIIMDSNE
                     SKLGADGKPIYDANGKFLKGPDYWKPEPRIKALLNEAGMRDEGVSRGFE"
     gene            4387..5358
                     /locus_tag="AC158Q_11460"
     CDS             4387..5358
                     /locus_tag="AC158Q_11460"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3340434.1"
                     /translation="MRAIIATCIAMLAAAGSAHAAQGGGEAAYPTRPVRVLVPFPPGG
                     SNDIMARYLSQYLSERLGQQFIVDNRGGAQGIIATEIVANARPDGHTLLFVSAAHSVH
                     GGTRNKLPYHPINSFSWVGKLGFGPSVVSVHPGLKVASAKELIAYGRANPGKLTLATS
                     GGYALFASESFNYMSGMKMVTVVYKGGFPALVDVMGGQAQVNIGSLIQTLPYMSQGRV
                     RPLATTGAKRAAAAPDLPTVAESGVPGYEANNWWSVAAPAGTPTVIVNKLTAEMARYL
                     KQPETLKRFAAEGVEADYLNPAELRKLLPVEMEKWREVAKAANIKVE"
     gene            5432..6112
                     /locus_tag="AC158Q_11465"
     CDS             5432..6112
                     /locus_tag="AC158Q_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340435.1"
                     /translation="MAASAESQPVSRSGTAVSVMLLVAYVTVVAWVFYEQSASWPHEG
                     SMAYLVLFLLCPGIVFQSVSLAYNWRTRLRLTRRALTRLVTIPLGLVIAAVLASWAST
                     LAMSGFEQAYVPFVAKVGANPADACRSAVEFFGISSVAAYNRRAGREHPFGKLHHDGK
                     RFVLAFHGGSADIDGSTISYDSGARTWRKFHNDNSSAREAYAGLTAGLAECPLRAQQA
                     TAGGAVMK"
BASE COUNT         1063 a         1985 c         2127 g         1121 t
ORIGIN      
        1 gaccaccacg tgcggccccg gcgtctcgca gctcgccacc gcgctgatca ccgccagccg
       61 cgccgcgtcg ccggtcgtgg cgttttgcgg cgagtacccg aataacgacg acgagtacaa
      121 ccagcgcatg gaccagcagt cgttcgccgc cggctgcgaa acggccttca tccgcatcac
      181 gtcgcccgac atcgcggacg attgcgtgcg caaggccttc tacaccgcca agctggaatc
      241 gcgccctgtc atgttgagcg cgcccatgga cgtgcagcag atggctttcg aggacgacga
      301 cgaagcgtac aagccgtcct ccacgatcct gccgaagcga accatccacc cggacccgga
      361 cgagatcgcc cgggccgccg acctcatcgg caaggccaag cacccggtga tcatctgcgg
      421 gcgcggcgcg atgtgggcgg gcgcgggccc gtcggtcgtc aagctcgccg accggatcgg
      481 cgcgctgatc ggcacctcgc tgatggcgaa gggctggctc gccgagcagg agtggtacgc
      541 cggcatctcc ggcacctacg gcgcgcgcaa ctcgttgaag ctcttcgagg agtcggactg
      601 cgtcatcgca gtgggcgcga ccatgaaccg ctacaccacc gagcacggct acttgtatcc
      661 gaacgcgaaa ttcgtgcacc tcgacgtgaa gccgcatctg gtgatgagcg gcggccgcgc
      721 ggccgattgt tacgtgcaga ccgatgcgcg ggcgggagtg gaagcgctcg aggcggagct
      781 ggcgaagcgc caggtcagga tgacggggta ccgcacgtcg gaagtgaaga agctgcttgc
      841 cgcgcgcaac gtggatcgtg ccgagttccc gatcgagccg gaccacgtgg acgcgcgcga
      901 gatcatccag acgctggatg agatgctgcc gcccgcgatc ggagtcgtca cgggcagcgg
      961 attcacagca ggtttctcga acatgctctt caacaagccg cgatcgctag tgctgccagg
     1021 gcatttcttc ggctgcatcg ggcagatgct gcccgcggcg atgggggtgg tggtggcgag
     1081 cggcaacaag ccgcacgtgc tgtgcgacgg cgacgccagc gtcatgatgc acatcggcga
     1141 attcgagacg atggtgcgct acaagatgcc gctgatggtg gtgtgcctca acaaccaggc
     1201 gctgggctcg gagtactaca agctcgacgc gcacaagatg aaggcggaat tgtcggtcgt
     1261 ggagacgccg gacctggggc aggtcggaaa agccttcggc ggacgcggca agctggtgag
     1321 aagcgtggaa gacctcaaga cggcgacgca ggaattcctc gccagccccg gcccgatgct
     1381 gctcgacgtg cgcatttccc gcagcgtggt caccctgccg taccgccgca ttcattacgg
     1441 gcgggacgag taaaacctag gtacaaggaa caaggtgcaa ggcgcaggtc taaaaacctt
     1501 gaaccttgaa ccttgaacct tgaaccttga accttgaacc tgctttatcg acagcggggt
     1561 ttcatccagt tcctggcgca actcgtggtc gcgctcatcc tgctcgcgct cgcggcgcgg
     1621 ttcgtctggt gggtcgcgac ggagacgaac ttcgaccagg ttgccacgat cctgtggggg
     1681 ctgggcttcg tcgccgctta cttcatcacg ctcgggaatt atttcgtgct gcgcatgccg
     1741 gcgggcttcg gcgtgttcgc gctgatcggg ctcgcgtcgc tgccgctgtc gtggctggcc
     1801 gcaagccggg tcgcgctgct cgtgctgctc gccgcgcagc tcgcattcag tttttacgcg
     1861 ggccgcgcga tttcggtccg cctgcgcgcg cgccattccg aaatggaaga ggacgaggcg
     1921 gcccgaaaag gcgccggcct ggtcaccggt ttggggcgtt gggccatgct ggctcttgcc
     1981 gccctgatgg tgctcctgat cgggccgctg ctggtattgg cgttggtctc ggtggcggtg
     2041 gatctgtcgg atcaggaact cagatggctc gccgcgctgt ggggtctcgc gggggtcgcc
     2101 tggtttggct accgcttcgg tgtcgtgaag tggcggcgag tcccggcttg cgcgtggatt
     2161 tacctgctgg cgaccgtgcc gatcctcgtc gccgacgcgc tggccgggcc cttcgcggaa
     2221 ggcgccggcg tccaggtcgc ctataccacc atgcccggcg cgctcgcagc cgcgttcgtc
     2281 gaggtgttcg tcttcggagg cggccagctg gatcctgacg accggctgcc tgattcgtga
     2341 atcgtaattc gtgaaatata gatgtggtac tgtaaaacat cgaaagacag acgaacgcga
     2401 ttccagcaca ctcacgccct atgaccacac cctcccaggc cgccacgacc cataacgggt
     2461 ttccacccat cgtgagcctg ctgctcaacg tcgctcacgg catcgaccac atgttcctgc
     2521 tgatcttcgc cacggcggtc gcttccatcg ccgtcgaatt cggctacgcc acctggacgg
     2581 acctgatgcc gtacagcgtg ggggccttcg cgctcttcgg cctcggtgcg ctgcccgccg
     2641 gccgcctggg cgacgtgtgg ggccgccgca gcatgatgat cgtcttcttc atcggcattg
     2701 gcgcggccgc cctgctgacg gcgctgacac agaacgcatg gcagctcgcc gccgcgctga
     2761 cgctgctggg cgccttcgcc gcaatctacc acccggtggg catcccgatg ctggtgcaga
     2821 acgcgcccaa cccgggcgcc gtgatcggcg tgaacggcct ggccggcaac ctcggcatcg
     2881 ccgtcgccgc gctcgtgacc ggcttcctgg tcaagtggat cggctggcgc gccgccttcg
     2941 cgatacccgg gctcatcgcg atcggttgcg ggatcgtctt cgcgatcgta tgtccgaaag
     3001 aggagcacgc gccgtccaag ggcagggggg gcaaggccaa ggtggtgctg acgcccgcca
     3061 tgctggtacg agcgttcgcg gtcatgacgg cggcggcggc caccagcagc atccttttca
     3121 acttcacgac caacggcaat tcgcagctcc tgtccgagtc gtttcgcggc gtgatcgagg
     3181 acccggccag gctgggcgcc ctgctggcgg tgatttacac cgtcgcctcg ttcgcgcaga
     3241 tcgtcgtcgg gcgtctgatc gacgtcatgc cgttcaagcc gctgcagctg tggatgtcgg
     3301 tcgcccaggt gccgctgctg atcatcgccg cgtacacgca ggactggggg atgttcgtcg
     3361 cgctgatgct ggtgatgatc ttcatcttcg gctcgatccc gttcaccgac gccatgatcg
     3421 tgcgctacgt ggacgaccgc ctgcgatcgc gggtggccgg catgcggctg acggtggcgt
     3481 tcggcatcag ctcggccgcg gtctggctgc tggggccgat ggtgaagagc gtgggcttcg
     3541 ccacgctgct gtggatcatg gccggtatcg ccgcggtaaa ggccgcgatc gtgctgttcc
     3601 tgcccgacga atcctcgtcc acggtcgggg cggcccggac gtgacgtcaa tgacgccgtg
     3661 atggagtggc gcggcgaggt taaatccggc gccgcgggtc gtcacttact cgctattctt
     3721 acggctgaat ccattaggta accgataccg ccgacatgaa gaaatcattc gcacagcgca
     3781 tcgccgcgat gaacgcgatg tacaggctcc ccgccggcga tcgcccgggg ttgcccgcgg
     3841 acgtggccga ccggttggtc aagttcaagg ccaccctgct cgacgaggtg cacgagatcg
     3901 acgagatcgt ccagctgtcg aaaaacggcg gcgcggcgct ggacgtcgcg gttgccatcg
     3961 cggacctgct gggcgatatc acggtgtact gccgctccga ggccctgaag tacggcctgc
     4021 cgctggagga agtgctcgac atcatcatgg acagcaacga aagcaagctc ggcgcggacg
     4081 gcaaacccat ctacgacgcc aacgggaagt ttctgaaggg gccggattac tggaaaccgg
     4141 aacccaggat caaggcgctc ctaaacgagg cagggatgag ggatgaggga gtgagccggg
     4201 gtttcgaata acgtgcctgc gcaggttaga gtcggcgaac gcccgggacg atgcagcgtt
     4261 tcgggacgga gggaaccagg tgtgctgttg gcagcggcag caaactggcg gctgctaact
     4321 gtctacttgt tccatccgcc attgcgccgc aggcgtaaga tgcggtggga cagacaaggg
     4381 ggtaacgtga gagccatcat cgcaacgtgt attgccatgc ttgccgcggc gggcagcgct
     4441 catgcggcgc aggggggcgg ggaagcggcc tatccaacgc ggccggtccg ggtgctggtg
     4501 ccgttcccgc cgggtggcag caacgacatc atggcccgct acttgtccca gtacctcagc
     4561 gagcggctcg gccagcagtt cattgtggac aatcgcggcg gtgcgcaagg gatcatcgcc
     4621 accgagatcg tggcgaacgc gcgccccgac gggcacacgc tgctgttcgt ttcggccgcg
     4681 cactcggtgc acgggggcac acggaacaag ctgccttatc acccgatcaa ctcgttcagc
     4741 tgggtcggca agctcggatt cggccccagc gtggtgtcgg tccacccggg gctcaaggtc
     4801 gccagcgcga aggagttgat cgcgtacggc cgcgcgaatc ccggcaagct gacgctggcc
     4861 acttcgggcg ggtacgcctt gttcgcctcc gagagtttca attacatgtc cggcatgaag
     4921 atggtgaccg tggtctacaa gggcgggttt cccgcgctgg tggacgtcat gggcgggcag
     4981 gcgcaggtca acataggctc gctgatccag acgttgccgt acatgtcaca ggggcgggtg
     5041 aggccgctgg cgaccaccgg cgcgaagcgc gccgccgcgg cgcccgacct gccgaccgtc
     5101 gccgaatccg gcgtgcccgg ctacgaggcg aacaactggt ggtccgtcgc cgcgcccgcc
     5161 ggtaccccga cggtgatcgt caacaagctc accgccgaga tggcccgcta cctcaagcag
     5221 cccgagacgc tgaagcgttt cgcggcggag ggagtggagg cggactacct caatccggcg
     5281 gagctgcgca agttgctgcc ggtcgagatg gagaagtggc gcgaggtcgc gaaggcggcg
     5341 aatatcaagg tggagtgacc agtgtcgggt gacagtgacg agtaacgagt aacgattatc
     5401 aggcgcaccc ttagcgctcg cgtgaacgtc gatggcagcc agcgccgaat cgcaacccgt
     5461 ttcccggtcg ggcaccgccg tcagcgtgat gctcctcgtg gcgtatgtga cggtggtggc
     5521 gtgggtcttc tacgagcaat ctgcctcgtg gccgcacgag gggagcatgg cttacctggt
     5581 cctgttcctc ttgtgtccgg gtatcgtgtt ccagtccgtc agcctggctt acaactggcg
     5641 cacgcggctc cggctcactc gccgcgccct gacccggctc gtgaccatac ctctggggct
     5701 ggtcatcgcg gccgtgctgg catcctgggc gagcacgctc gccatgagcg gcttcgagca
     5761 ggcctacgtg ccgtttgtcg ccaaggtcgg cgcgaacccg gccgacgcgt gtcgttccgc
     5821 cgtcgagttt ttcgggattt cttccgtggc ggcctacaac cggcgggcgg ggcgcgagca
     5881 tcccttcggg aaactgcatc acgacggcaa gcgcttcgtc ctcgcgttcc atggcggctc
     5941 ggcggacatc gacggcagca cgatttccta cgattcaggc gccaggacgt ggcgcaaatt
     6001 tcacaacgac aacagcagcg cgcgggaagc ctatgccggt ctgacggcag ggctggccga
     6061 atgcccgctg cgcgcgcagc aggcaacggc tggtggtgca gtcatgaagt gacgcagtga
     6121 tgaagcgtat ttcattgccg cattactgca tcaggagact ttgagtccca cgtgaaactc
     6181 ccccacatag cccgcaccgc tcccggctaa cgcaagcatc aggcggttac cgccgcggcg
     6241 gaacaggccg tctttgacat tgagggtgcg tgagcgcgct ccgcggatgg agtagg
//
LOCUS       DBNGSG010000442         7115 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2012704, whole genome shotgun sequence.
ACCESSION   DBNGSG010000442 DBNGSG010000000
VERSION     DBNGSG010000442.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7115)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7115)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7115
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2012704"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="AC158Q_11470"
     CDS             <1..192
                     /locus_tag="AC158Q_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340436.1"
                     /translation="IADIVGHSDVAPGRKSDPGPYFDWARYRGMLEINRQNAKAAKKT
                     STRSLTAKTPRHQEKGKKK"
     gene            622..3495
                     /locus_tag="AC158Q_11475"
     CDS             622..3495
                     /locus_tag="AC158Q_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HIR3340437.1"
                     /translation="MQLATELAPVTPAVALTPNDTPAAREARYAQYRVIRRNGAVVGF
                     EPSKISIAMTKAFIAVNGGQGAASARVRDQVTILTENVVIALMRRQPSGGTFHIEDIQ
                     DQVELALMRAGEHNVARDYVLYREERARERAKQRQAQEKEKGNRAQPPALNVKEGGQM
                     RPLDLDRITALVREACEGLGRDVGAEPIMQAMQRDLYDGVPMEEVRKSLVLAARALIE
                     QDPGYSYVTARLLLHTLRLETLGVEVAQGEMNARYAEYLPEFIRKGIEAELLDERLAH
                     YDLKCLGAALDANRDLKFTYLGLQILYDRYFLHVRGRRIELPQVFYMRVAMGLALNEP
                     EDQREARAIEFYNVLSSFDFMSSTPTLFNSGTRRSQLASCYLTTVADDLDGIYEAIKE
                     NALLQKFAGGIGNDWTPVRALGSHIKGTNGKSQGVVPFLKVVNDTAVAVNQGGKRKGA
                     VCSYLETWHLDIEEFLELRKNTGDDRRRTHDMNTANWVPDLFMKRVMENGEWTLFSPS
                     DALDLHDKFGRAFEKAYLECEARAARGEIKPYKKIQATQLWRKMLTMLFETGHPWITF
                     KDPCNLRSPQQHVGVVHSSNLCTEITLNTGPDEIAVCNLGSVNMPAHLDVSTGRMDME
                     KLRRTVGTAMRMLDNVIDLNFYSVNKARNSNFKHRPVGLGIMGFQDCLHMLRIPYGSQ
                     AAVEFSDRSMEAIAYTAYQASSDLAEERGPYSTFKGSLWDRGILPFDTLKLLAEERGG
                     YVECDMSTTLDWESLKKRVQQVGMRNSNCLAIAPTATIANITGLSQCIEPTYQNLYVK
                     SNLSGEFTVVNEYLVRDLKKLNLWDEVMIADLKYFDGSLSKIDRIPPEVRNLYATAFE
                     VEPKWLVECAARRQKWVDQSQSLNIYMAGASGKKLDDTYKLAWIRGLKTTYYLRTLGA
                     TSAEKSTVTTGQLNAVNADGGLTAPATDAKFCSIDNPECESCQ"
     gene            3538..4659
                     /locus_tag="AC158Q_11480"
     CDS             3538..4659
                     /locus_tag="AC158Q_11480"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011401972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HIR3340438.1"
                     /translation="MLQFGEQQTIGAGTGGGIQPGARAPVPQPEINAELKRVNVADKR
                     VINGSTDVNQLVPFKYKWAWEKYLSACANHWMPQEIQMSRDIALWKDPHGLTEDERRI
                     VKRNLGFFVTADSLAANNIVLGTYRHITAPECRQYLLRQAFEEAIHTHAYQYIVESLQ
                     LDDGEIFNAYHEIPSIRAKDEFLIPFINTLTDPAFKTGTPESDQKLLKSLIVFACLME
                     GMFFYVGFTQILSLGRQNKMTGSAEQYQYILRDESMHCNFGIDLINTIKMENPHLWTP
                     DFRDELREIFKQGVELEYRYAEDTMPRGVLGLNAGMFKEYLRYICNRRSQQIGLEPLF
                     EGVSNPFPWMAEMIDLKKERNFFETRVIEYQTGGALNWD"
     gene            4719..5024
                     /locus_tag="AC158Q_11485"
     CDS             4719..5024
                     /locus_tag="AC158Q_11485"
                     /inference="COORDINATES: protein motif:HMM:NF021873.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HIR3340439.1"
                     /translation="MGSSLLNRRYRRRRQYLHTVIECRAEGNYTLWVKFDDGLEGLVY
                     LGDLVTTNSYLALSDEDIFSRVGIDPVSNAVTWTGGIQLEPEVLYRDLASKAHAAWH"
     gene            5074..5256
                     /locus_tag="AC158Q_11490"
     CDS             5074..5256
                     /locus_tag="AC158Q_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340440.1"
                     /translation="MAKAKKRAGIKKSAKKRKPVARRRPAAKRTAAKRGPTAAAPMAP
                     RPVVIPGAWPFPKGSK"
     gene            5359..5571
                     /locus_tag="AC158Q_11495"
     CDS             5359..5571
                     /locus_tag="AC158Q_11495"
                     /inference="COORDINATES: protein motif:HMM:NF025005.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HIR3340441.1"
                     /translation="MERKRVSASNIRSVGYDAVKQLLEIEFSGGAIVQYSGVSPEVHR
                     RFLNSPSPGSFFQDQIEENFTGRKIR"
     gene            complement(5708..6739)
                     /locus_tag="AC158Q_11500"
     CDS             complement(5708..6739)
                     /locus_tag="AC158Q_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004793 - threonine aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0006567 - L-threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HIR3340442.1"
                     /translation="MIDLRSDTLTRPTEAMLESMREATFGDDSRDGDATVQKLEAMAA
                     ERTGKEAGAFMPSGTMTNLVAMLAHAQRGGEVLLEEGSHTLNAELGGIAGVAGLFYRG
                     IPGKRGAMDVEVLREAIRPVTRHHMGTALVWMENTHNRAGGAVLPLSHMRAVYTLARE
                     NNVPVHLDGARIFNAAHALKTTPKDIAQYSDSVCFCVSKGLSAPVGSILCGSGEFIER
                     ARAHRRMVGGNLRQAGPLAAAGIVALETMVNRLPEDHQTAKRLAEGFNRIDTSLCEPK
                     DVETNLVRVDVKKSGRRAAQWSADLKAKGVMVAPADPWTLRFVTHRHISAPDVDTAVS
                     AFAELWTKI"
     gene            complement(6846..>7115)
                     /locus_tag="AC158Q_11505"
     CDS             complement(6846..>7115)
                     /locus_tag="AC158Q_11505"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3340443.1"
                     /translation="WFGDYDKLAMTRRLMREAFRVDLDTAREHHVFVGDSPNDAPMFA
                     FFPHAVGVANVREFAGRLAHEPKYVTTASSGAGFCELADFLLSCR"
BASE COUNT         1479 a         2227 c         2210 g         1199 t
ORIGIN      
        1 atcgccgata tcgtcggaca ttccgatgtc gcgccgggga ggaagagcga cccggggccc
       61 tatttcgact gggcgcgcta ccgcggaatg ctggaaatta accgccagaa cgccaaggcc
      121 gccaagaaaa ccagcacaag atcactaacc gccaagacgc caagacacca agaaaaaggg
      181 aagaaaaaat agcagccgag ccgatgcgaa ttttcctgtg agaatcaccg ccgcgcgttg
      241 aacggcgagc ccatcgtccg ttcgaacgag cggagttaaa acagcgctcc gcgtctgttt
      301 taaccctgtt tatccacagc ccctggcccg accggccccg ccgtttctag cggaccagac
      361 cactgctcaa aacatgagca gttttgccca ttgcttcatc accttgcggg cggtttctcc
      421 caattaatcg atgatttaag aaatacttca aataccctat tgcgttcgta aaatcaggct
      481 actagaatta gtcagaaaat tgccggtgac cacaacatat tgtggttgat gttggtggat
      541 acgggctcat aaaggttttg gactggggag ttagccctcc ccttgggtga aaaaaaaact
      601 taaaactcaa ggagttaata aatgcagctc gccactgaat tggcccctgt taccccggcc
      661 gttgccctta ccccgaacga tacccccgca gcgcgcgaag ctcgctacgc gcagtaccgg
      721 gtaatccgcc gcaatggcgc cgtcgtcggc ttcgagcctt cgaagatttc catcgccatg
      781 acgaaggcat tcatcgcggt gaacggcggc cagggcgccg cttccgcgcg cgtgcgcgat
      841 caggtgacga ttctcaccga aaacgtggtg atcgcactga tgcgccgcca gccctcgggc
      901 gggactttcc acatcgagga catccaggac caggtggagc tggcgctgat gcgcgcgggc
      961 gagcacaacg tcgcgcgcga ttacgtgctc taccgcgagg aacgggccag ggaacgcgcg
     1021 aagcagcgcc aggcgcagga gaaggaaaag ggcaatcgcg cgcagccgcc ggcgctgaac
     1081 gtgaaggaag gcgggcagat gaggccgctc gacctcgaca ggatcacggc gctggtgcgg
     1141 gaggcatgcg aggggctcgg gcgcgatgtg ggcgcggagc cgatcatgca ggcgatgcag
     1201 cgcgacctct atgacggcgt gcccatggag gaagtgcgca agtcactcgt cctcgccgcg
     1261 cgcgcactga tcgagcagga cccgggctac agctatgtca ccgctcgcct gctgctgcac
     1321 acattgcggc tcgagacgct gggcgtggag gtggcgcagg gagagatgaa cgcccgctac
     1381 gccgagtacc tgccggagtt catcaggaag ggcatcgagg ccgagttgct ggacgagcgc
     1441 ctcgcccact atgacctgaa atgcctcggg gcggcgctcg atgccaaccg cgacctcaag
     1501 ttcacctatc tgggattgca gatcctctac gaccgctatt tcctgcacgt gcgcggccgc
     1561 cgcatcgagc tgccgcaggt tttctacatg cgcgtggcga tggggctggc gctgaacgag
     1621 cccgaggacc agcgcgaggc gcgcgcgatc gagttctaca acgtgctgtc ctcgttcgac
     1681 ttcatgagct cgacgccgac gctgttcaat tccggcacgc gccgctcgca gctcgcgagc
     1741 tgctacctga cgaccgtggc agatgacctc gacggcattt acgaggcgat caaggaaaac
     1801 gcgctgctgc aaaagttcgc cggcggcatc ggcaacgact ggacgccggt gcgcgcgctg
     1861 ggttcccaca tcaagggcac caacggcaag agccagggcg tcgtgccgtt cctgaaggtg
     1921 gtgaacgaca ccgcggtggc ggtcaatcag ggcgggaaga ggaagggcgc tgtgtgctcg
     1981 tacctcgaga cctggcacct cgacatcgag gaattcctcg agctgcgcaa gaacaccggc
     2041 gacgaccgcc gtcgaaccca cgacatgaac actgccaact gggtgcccga tcttttcatg
     2101 aagcgcgtga tggaaaacgg cgagtggacg ctgttctcgc cctccgatgc gctggacctg
     2161 catgacaagt tcgggcgcgc tttcgagaaa gcctacctcg aatgcgaggc gcgcgccgcc
     2221 cgcggcgaga tcaagcccta caagaagatc caggcgacgc agctatggcg caagatgctg
     2281 acgatgctgt tcgagaccgg gcatccgtgg atcacgttca aggacccgtg caacctgagg
     2341 agcccgcagc agcacgtggg cgtggtgcac agctccaacc tctgcaccga gatcacgctg
     2401 aacacggggc ccgacgaaat cgcggtgtgc aacctgggct cggtcaacat gccggcgcac
     2461 ctcgatgttt ccaccggccg catggacatg gagaagctgc gccgcaccgt cggcaccgcg
     2521 atgcgcatgc tcgacaacgt catcgatctc aacttttact cggtcaacaa ggcgcgcaac
     2581 tccaatttca agcaccgccc ggtgggcctc ggcatcatgg gcttccagga ctgcctgcac
     2641 atgctgcgca tcccgtacgg ctcgcaggcc gcggtggaat tctcggatcg ctcgatggag
     2701 gcgatcgcgt acaccgcgta ccaggcgtcg agcgacctcg cggaagagcg cgggccgtac
     2761 tcgacgttca agggctcgct gtgggaccgc ggcatcctgc cgttcgacac gctgaagctc
     2821 ctcgcggaag agcgcggcgg ctacgtcgag tgcgacatgt ccaccacgct cgactgggaa
     2881 agcctcaaga aacgcgtgca gcaggtcggc atgaggaact cgaactgtct cgcgatcgcg
     2941 cccaccgcga ccatcgccaa catcaccggg ctgtcgcagt gcatcgagcc gacctaccag
     3001 aatctctacg tgaagtcgaa cctgtcgggc gagttcacgg tggtgaacga gtacctggtg
     3061 cgcgacctga agaagctcaa cctgtgggac gaggtcatga tcgcggacct gaagtacttc
     3121 gacggctcgc tctcgaaaat cgaccgcatt ccaccggaag tccgcaacct ctacgcgacc
     3181 gcgtttgagg ttgagcccaa gtggctggtc gagtgcgcgg cgcggcgcca gaagtgggtg
     3241 gaccagtcgc agtcgctcaa catctacatg gcgggcgctt cgggcaagaa gctcgacgac
     3301 acctacaagc tggcgtggat ccggggtcta aagaccacgt actaccttcg caccctcggg
     3361 gcgacctcgg cggagaagtc caccgtcacg accgggcagc tgaacgcggt gaacgccgac
     3421 ggcggactca cggcgccggc aacggacgcg aagttctgct ccatcgacaa cccggaatgc
     3481 gagtcctgcc aataggcagc tggatccacc cataacaccc ataaaggaga ctgaaagatg
     3541 ctgcaattcg gggaacagca aacgatcggc gccgggaccg gcggcgggat tcaacccggc
     3601 gcgcgtgcgc ccgtgccgca gccggaaatc aacgcggaac tgaagcgcgt caacgttgcc
     3661 gacaagcgcg tcattaatgg cagtaccgac gtcaaccagc tggtgccgtt caagtacaag
     3721 tgggcatggg aaaagtatct ctccgcctgc gccaatcact ggatgccgca ggaaatccag
     3781 atgagccgcg acatcgcgct gtggaaggat ccgcacggtt tgaccgagga cgagcggcgc
     3841 atcgtcaagc gcaacctcgg cttcttcgtg accgcggact cgctcgcggc gaacaacatc
     3901 gtgctcggca cctatcgcca catcacggcg ccggaatgcc gccagtacct gctgcggcag
     3961 gcgttcgagg aggcgatcca cacccacgcc taccagtaca tcgtggagag cctgcagctg
     4021 gacgacggcg agatcttcaa tgcctaccac gagattccct cgatccgggc caaggacgaa
     4081 ttcctgattc ccttcatcaa tacgctgacc gatcccgcgt tcaagaccgg cacgccggaa
     4141 tcagaccaga agctcctgaa gagcctgatc gtgttcgcgt gcctgatgga gggcatgttc
     4201 ttctacgtcg gctttaccca gatcctctcg ctcgggcgcc agaacaagat gaccggatcg
     4261 gccgagcagt accagtacat cctgcgcgac gaatccatgc actgcaattt cggcatcgat
     4321 ctcatcaaca cgatcaagat ggagaacccg cacctgtgga caccggattt tcgcgacgaa
     4381 ctgcgcgaga tcttcaagca gggggtcgag ctcgagtacc gttacgccga ggacaccatg
     4441 ccgcgcggcg tgctgggatt gaacgccggg atgttcaagg aatacctgcg ctacatctgt
     4501 aaccgccgca gccagcagat cggcctcgag ccgctgttcg agggtgtttc caatccgttc
     4561 ccgtggatgg cggaaatgat cgatctcaag aaggagcgca attttttcga aacgcgcgtc
     4621 atcgaatatc agaccggggg ggcgctcaac tgggattaac gcgggcgctg cgcaccgggc
     4681 aagggggcat tcatcaacca acagggaggc gcgctatctt gggttccagc ctgctgaatc
     4741 gacgttaccg cagacgcaga cagtacctgc acaccgtcat cgaatgccgc gccgagggca
     4801 attacaccct ctgggtcaag tttgacgacg gcctggaagg gctggtctac cttggcgacc
     4861 tggtcacgac gaattcgtat ctggcgctct ccgacgaaga cattttctcg cgcgtcggga
     4921 tcgacccggt gtcgaacgcg gtgacctgga ccggcggcat ccagctcgaa cccgaggtgc
     4981 tgtaccgcga cctcgcgagc aaggcccacg cggcttggca ttagggccat tgagttgagc
     5041 cgccgccgcg caggcaaccg actggagggc tgaatggcga aagcgaagaa acgggccggg
     5101 atcaagaagt cggcgaagaa aaggaagccg gtggcgcggc gcaggccggc cgcgaagcgc
     5161 acggccgcaa aacgcgggcc gaccgccgcc gccccgatgg cgccgcgccc ggtggtcata
     5221 cccggcgcct ggccgttccc gaagggcagc aagtaggtcc ggacgcgcgg cgccggcctg
     5281 tctccgctcg ttcgaacgga cggactaacc accgttcaat gcgcggccga tgggttccct
     5341 tgtggtcctc gagaatacat ggagcgtaag cgagtcagtg cgtcaaacat tcgctcggtc
     5401 ggctacgacg cggtaaagca gctcctggag atcgagtttt ccggcggggc catcgtgcag
     5461 tactccggcg tgtcgccgga agtgcaccgc cggttcctga attcgccctc gcccgggagc
     5521 ttctttcagg atcagatcga ggagaacttc accggcagga aaatccgctg aatcagccgc
     5581 caggacgcca agtaaaccaa taatcgaacc gccaagagcg ccaccaaaac tggccaagac
     5641 ggctacgagt tgttcgaaga ggcgggttga tagttttcaa atcgtcttca tagccgttca
     5701 tcggcggcta aatcttcgtc cacagctccg cgaaggcact gacggcggtg tccacgtccg
     5761 gtgcggaaat gtggcggtgg gtgacgaagc gcagcgtcca ggggtccgcc ggcgccacca
     5821 tgactccttt cgccttgagg tccgccgacc attgtgcggc gcgcctgccg ctcttcttca
     5881 cgtccacccg caccaggttg gtttcgacgt ccttcggttc gcacaggctc gtgtcgatgc
     5941 ggttgaatcc ttccgcgagg cgcttcgcgg tctgatggtc ctcgggcagg cggttcacca
     6001 tggtctccag cgcgacgatg ccagcggcgg caagcggccc cgcctggcgc aggttgccgc
     6061 ccaccatgcg gcggtgggcg cgcgcccgct cgatgaactc gccgctcccg cagaggatgg
     6121 agccgaccgg cgccgacaac cccttcgaca cgcagaaaca taccgaatcc gaatactgtg
     6181 caatatcctt cggcgtagtc ttgagcgcgt gcgccgcgtt gaagatgcgc gcgccgtcga
     6241 ggtgcaccgg gacgttattc tcgcgcgcga gcgtgtatac cgcgcgcatg tgcgagagcg
     6301 gcaataccgc accgcccgcg cggttgtggg tattttccat ccagaccagc gccgtcccca
     6361 tgtggtggcg cgttacgggc cggatcgcct cgcgcagcac ttccacgtcc atcgcgccgc
     6421 gcttgccggg gataccgcgg tagaacaggc ccgcaacgcc ggcgataccg cccagttcgg
     6481 cgttcaatgt gtgcgagcct tcttcgagca acacctcgcc gccgcgctgg gcatgcgcga
     6541 gcattgccac caggttggtc atggtgccgc tcggcatgaa ggcgccggct tccttgccgg
     6601 tgcgctctgc ggccatcgcc tccaatttct gcaccgtggc gtcgccgtcg cgcgagtcgt
     6661 cgccgaaggt cgcctcgcgc atggattcga gcatggcctc ggtgggccgg gtgagcgtgt
     6721 cgcttcgcag atcgatcatg ccgtaaacct ttgaattagc cgccaagacg ccagggcgcc
     6781 aagcaggaca ggacaataaa tctgaccgcc aagatcgcca agactaccaa gaaaatagaa
     6841 ttgattcagc ggcatgacag gagaaaatcg gcgagctcgc aaaaaccggc accgcttgaa
     6901 gccgtggtta cgtattttgg ctcgtgggcg agccggcccg caaactcgcg gacgttggcc
     6961 acgccgaccg cgtgggggaa aaaagcgaac atcggcgcat cgttgggcga gtcgccgacg
     7021 aaaacatggt gctcacgcgc ggtatcgaga tcgacgcgga acgcttcgcg catgagccgc
     7081 cgcgtcatgg cgagcttgtc gtagtcgccg aacca
//
LOCUS       DBNGSG010000443         4462 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1672799, whole genome shotgun sequence.
ACCESSION   DBNGSG010000443 DBNGSG010000000
VERSION     DBNGSG010000443.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4462)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1672799"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="AC158Q_11510"
     CDS             <1..699
                     /locus_tag="AC158Q_11510"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3340444.1"
                     /translation="LASKSLLTNNIAGRGPSYTEFTPVAFLFGEYVSVTVKPDSPLKT
                     GRDLIERMKQDPASLSFGIATSLGNPNHQAVAAALKAAGVDIRKLRTVIFSSGGAAST
                     AMLGGHVDVVPITAAFAASMARQGQIRVIAVTAPSRLPDVLADVPTWRELGYDAVVSN
                     WRAIVGPKGMTPPQVAYWEKILARFTESDGWKQELEKNFWRSEYLRSAETRKYMDQDN
                     VQAREFLVELGLAK"
     gene            complement(796..2091)
                     /locus_tag="AC158Q_11515"
     CDS             complement(796..2091)
                     /locus_tag="AC158Q_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340445.1"
                     /translation="MDTAAASSAPLAHRVKRLGHLDLPGGGQVVVQGHHAFVGHMDAP
                     HGTTILDVSDPRKPRVVAEIKLETADEHSHKVRVVGDIMYTNVERPSRRFAAKAVRLA
                     QTRADMEQALGRPPTAAELADRIRVTPPQLAQLEEWSRNGFPNAGIRVWDVSNVAKPR
                     RIAHIRTSGVGAHRFDVDADYAYISTGMDGYGGNILVNYDVKDPARPQEVSRWWLPGQ
                     HIAGGETAEGPYEDWHLHHAMRCGDLLWAGCFNAGLRVIDVSDIRKPRTVGAYNYHPP
                     IGDPTHTVLHVPNRIAGREVALAIDEAHGRHHGRIPAGLWLFDVGDLARIRPLGHFQM
                     SELDTPWARKGGRFGAHQFQEHVEGTLVYCTWFSGGLRIVDIGDPLAPEEVAWFIPEP
                     CGGHATPQTNDVEVDARGLVYLVDRNCGFDILELTGGTA"
     gene            complement(2199..2927)
                     /locus_tag="AC158Q_11520"
     CDS             complement(2199..2927)
                     /locus_tag="AC158Q_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HIR3340446.1"
                     /translation="MRPDFPGIHEIWVYLSGSPLLALILTLTAYQVGVTVYERSRLNP
                     LANPVLIAVTLIALAITALDMPYDKYFEGAQFVHFLLGTATVSLAVPIYKGLPSLRGR
                     WLPLSAALLGGGVTSIVTAVVLARLLGADETIVGGLYAKSLTAPIGMGIAERIGVSPT
                     LTAVFTVTTGILGAALGRFVLDALRIREWWQRGYALGVASHGIGTSRAFSVNPEAGAY
                     AGLAMGLHGIAGAIAIPWAIGLLR"
     gene            complement(2917..3330)
                     /locus_tag="AC158Q_11525"
     CDS             complement(2917..3330)
                     /locus_tag="AC158Q_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HIR3340447.1"
                     /translation="MTNTGDGLHPSLIARRYRENGVIEGLVRILLFQGLGEVIAHFLL
                     PLVPGPVIGLILLLAFLVWRGRVNEGVELVSSAFMRYLGLLFVPAAVGVVLYWPHLQR
                     QALAIAVALLVSVIATIAVSALLAKWLDRNKTDAA"
     gene            complement(3338..4174)
                     /locus_tag="AC158Q_11530"
     CDS             complement(3338..4174)
                     /locus_tag="AC158Q_11530"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3340448.1"
                     /translation="MAIPSIPMLSVRGMQMPVIGFGTSQLGNCGEIVAQALKLGYRHI
                     DTAWKYGTEAGVGEGIKRSGVPRQDIFLVTKVSHEYLKADAFAKSVEESLQRLQTDYV
                     DLLFVHWPAIDGTPLAETMGALARAKREGKARHIGVANFNIAMTGDAMRLCPEPLTVL
                     QAEYHPYLDQSKVLAFCRKSGLAFMAYCPLGRGRLFKDPVLAEIARARSKTLAQVALR
                     WLVQQGNIAPIPRSSNPHHMAESLAVFDFSLTDDEMLRIHALARPDGRIANPAGRAPK
                     WD"
     gene            complement(4269..>4462)
                     /locus_tag="AC158Q_11535"
     CDS             complement(4269..>4462)
                     /locus_tag="AC158Q_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HIR3340449.1"
                     /translation="QDRKRLLLRLWLAVPGSRPLPRGYELLFGNIEAGAVRGGVPARE
                     GWRDVTQLRARKRAAAEKA"
BASE COUNT          758 a         1461 c         1549 g          694 t
ORIGIN      
        1 ctcgcctcca agtcgctgct caccaacaac atcgccgggc gcgggccgag ctacacggaa
       61 ttcaccccgg tcgcgttcct gttcggcgag tacgtctcgg tgacggtgaa gcccgactcg
      121 ccgctcaaga ccggccggga cctgatcgag cgcatgaagc aggatcccgc gtcgctctcc
      181 ttcggtatcg cgaccagcct cggcaacccg aaccatcagg cggtggccgc ggcgctgaag
      241 gcggccggcg tggacatcag gaagttgcgc accgtcatct tttcatcggg cggggcggca
      301 agcaccgcga tgctcggcgg ccacgtcgac gtggtcccga tcacggcggc cttcgcggcc
      361 tcgatggcgc ggcagggtca gatacgggtg atcgcggtga ccgcaccctc gcgcctgccc
      421 gacgtgctcg ccgacgtgcc gacgtggcgc gagctgggct acgacgccgt cgtgtcgaac
      481 tggcgcgcca tcgtcggccc gaagggcatg acgccgccgc aggtcgccta ctgggagaag
      541 atcctcgccc gctttaccga atcggacggc tggaagcagg agctcgagaa gaacttctgg
      601 cgcagcgagt atctgcgcag cgccgagacg cgcaagtaca tggaccagga caacgtccag
      661 gcgagggagt ttctggtcga gctgggactc gccaagtaac ccggtgacga gtgacgcgtg
      721 atgggtgacg ggatttcctc tctcccgggc ggggagagga gatcgattca cgaatcacga
      781 cttacgattc acgagtcacg ccgttccgcc ggttagctcg agaatgtcga agccgcagtt
      841 gcggtcgacg aggtagacca ggccgcgggc gtcgacttcg acgtcgttgg tctggggcgt
      901 ggcgtggccg ccgcacggct ccgggatgaa ccaggcgact tcctccggtg ccagcgggtc
      961 gccgatatcg acgatacgca gaccgccgga gaaccacgtg cagtacacca gcgtgccttc
     1021 gacatgttcc tggaactggt gggcgccgaa acgcccgccc ttgcgcgccc acggcgtgtc
     1081 gagttcgctc atctggaagt gaccgagggg ccggatgcgg gcgaggtccc ccacgtcgaa
     1141 caaccacagg ccggcgggaa tccggccatg gtggcgcccg tgcgcctcgt cgattgcgag
     1201 cgcgacctcg cgtcccgcga tccgattcgg cacgtgcagc acggtgtgcg tgggatcccc
     1261 gatcggcggg tggtagttgt aggcgccgac ggtgcggggc ttgcggatgt cggagacgtc
     1321 gatcacgcgc agcccggcgt tgaagcagcc ggcccaaagc aggtcgccgc agcgcatcgc
     1381 gtggtgcagg tgccagtcct cgtacggacc ctccgccgtc tcgccgccgg cgatgtgctg
     1441 gccgggcagc caccagcgcg agacctcctg cggccgggcc ggatccttca cgtcgtaatt
     1501 caccaggatg ttgccgccgt agccgtccat ccccgtggaa atgtaggcgt agtccgcgtc
     1561 aacgtcgaag cggtgcgcgc cgacgccgct ggtgcggatg tgcgcgatcc gtcgcggctt
     1621 cgctacgttg gaaacgtccc agacgcggat gccggcgttc gggaagccgt tgcggctcca
     1681 ttcctcgagc tgcgcgagct gcggcggcgt gaccctgatc cggtcggcaa gctcggcggc
     1741 ggtcggcggg cgcccgagcg cctgctccat gtcggcgcgc gtctgcgcga gccgcactgc
     1801 cttggcggcg aagcgccggc tcggccgctc gacgttggtg tacatgatgt cgccgacgac
     1861 gcgcaccttg tggctgtgtt cgtccgcggt ttcgagcttg atctcggcca ccacccgcgg
     1921 cttccgcgga tccgacacgt ccagtatcgt cgtgccgtgc ggcgcgtcca tgtggccgac
     1981 gaaggcgtga tggccctgca cgaccacctg gccgccgccc ggcaggtcga ggtgcccgag
     2041 ccgttttacg cggtgcgcca gcggcgcgga agaagctgca gcggtgtcca ttcaggttcc
     2101 tttcgttacc gagctacaaa ttcgcggagt gatggaacga tagcaaggcg aagggcggaa
     2161 gtcgaaaggc gaaaggaaaa cccgatcacc ggaatccatc agcgcagcaa cccgatcgcc
     2221 cacgggattg cgatcgcacc cgcgatgccg tggagcccca tcgccaggcc ggcataggcg
     2281 ccggcttcgg gattgacgct gaacgcgcgc gaggtgccga tgccgtggga ggcgacgccg
     2341 agcgcgtagc cgcgctgcca ccactcgcgg atgcgcaatg cgtcgagcac gaagcggccg
     2401 agggcggcgc ccaggatgcc ggtggtgacg gtgaacacgg cggtcagggt gggggagact
     2461 ccgatgcgct ccgcgatgcc catgccgatg ggggccgtaa gcgatttcgc gtagaggccg
     2521 cccactatcg tttcgtccgc gcccagcagc cgggccagca ccacggcggt gacgatggac
     2581 gtcacgccgc cgccgaggag cgccgccgag agcggcagcc agcgcccgcg cagcgacggc
     2641 agccccttgt agatcggcac cgccagcgac accgtcgcgg tgccgagcag gaagtgcacg
     2701 aactgcgcgc cctcgaagta cttgtcatac ggcatgtcga gcgccgtgat cgcgagcgcg
     2761 atcagcgtga cggcgatcag caccgggttc gcgagcgggt tcaaccgcga gcgttcgtag
     2821 accgtcacgc cgacctggta ggccgtgagc gtaaggatca gcgccagcag cgggctgccg
     2881 gagagataga cccagatttc gtggatgccg gggaagtcag gccgcatcgg tcttgttccg
     2941 gtcgagccac ttcgcgagca gcgccgagac cgcgatggtg gcgatgacgc tgacgagcag
     3001 cgccacggcg atggccagcg cctggcgctg caggtgcggc cagtagagca ccacgcccac
     3061 cgccgccggc acgaacagca ggccgaggta gcgcatgaac gccgaggaca ccagctcgac
     3121 accctcgttc acccggccgc gccagaccag gaacgcgagc agcaggatga gcccgatcac
     3181 ggggccgggc acgagcggca gcaggaagtg agcgatgacc tcgccgagac cctggaacag
     3241 caggatgcgc accagccctt cgatcacgcc gttctcccgg tagcgacggg cgataagtga
     3301 cggatgaagc ccgtcacccg tattcgtcac gcagacttca gtcccacttc ggcgcccgtc
     3361 ccgccggatt cgcgatgcgc ccgtcgggcc gcgcgagcgc gtggatgcgc agcatttcgt
     3421 cgtcggtcag cgagaagtcg aagaccgcca ggctctcggc catatgatgg gggttcgacg
     3481 agcgcggaat gggggcgatg ttgccttgct gcacgagcca gcgcagcgcc acctgggcaa
     3541 gcgtcttgct cctggccctg gcgatttccg ccagcaccgg gtccttgaac aggcgaccgc
     3601 gcccgagcgg gcagtaggcc atgaaggcga gcccggattt gcggcagaat gccagcacct
     3661 tggattggtc gagatagggg tggtactcgg cctgcaacac ggtgagcggc tcggggcaca
     3721 accgcatcgc gtccccggtc atcgcgatgt tgaagttggc gacgccgatg tggcgcgctt
     3781 tgccctcgcg cttcgccctt gccagcgcgc ccatggtttc ggcgagcggc gtgccgtcga
     3841 tggcgggcca atgcacgaac aggagatcca cgtagtcggt ctgcaggcgc tgcaggcttt
     3901 cctccaccga tttcgcgaag gcgtccgctt tcaggtattc atgcgagacc ttggtgacga
     3961 ggaagatgtc ctggcgcggc acgccggagc gtttgatgcc ctcgccgacg ccggcttcgg
     4021 tgccgtactt ccacgccgtg tcgatatggc ggtaaccgag cttcagcgcc tgcgcaacga
     4081 tctcgccgca gttgccgagt tgcgacgtgc cgaaaccgat gaccggcatc tgcatgccgc
     4141 ggacggacag catgggaatg gagggtatcg ccataagaaa ccggtgataa gtgataggtg
     4201 ataagtgata agtgataagg gttggtgaga gatggagtct aaaccagact cctatcacct
     4261 atcacccgtc acgctttttc ggcagcggcg cgtttgcgcg cccgcaactg cgtgacgtcc
     4321 cgccagccct cgcgagccgg cacgccgccg cgcaccgcgc cggcctcgat gttgccgaac
     4381 agcagctcgt agccgcgggg cagcggccgg ctgcccggca ccgccagcca caggcgcagc
     4441 agcaggcgct tgcggtcctg tt
//
LOCUS       DBNGSG010000444         6589 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1842690, whole genome shotgun sequence.
ACCESSION   DBNGSG010000444 DBNGSG010000000
VERSION     DBNGSG010000444.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6589)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6589)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6589
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1842690"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(342..875)
                     /locus_tag="AC158Q_11540"
     CDS             complement(342..875)
                     /locus_tag="AC158Q_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HIR3340450.1"
                     /translation="MRKKNRKAKPVTGHRSPTTLFRLAEKVGAALMARRLMLATAESC
                     TGGWIAEAITMVPGSSEWFERGFVTYTYISKREMLGVKDATLGKHGAVAEEVVREMAE
                     GALARSHAQVAVAVSGVAGPGGGTPEKPVGTVCFAWGMKRGRSRVETKRFAGNREAVR
                     RRSVEHALRGVLGMLAE"
     gene            complement(900..1394)
                     /locus_tag="AC158Q_11545"
     CDS             complement(900..1394)
                     /locus_tag="AC158Q_11545"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006644 - phospholipid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A family protein"
                     /protein_id="HIR3340451.1"
                     /translation="MSDLVLRPTWRFVLSRPAYLVAFGFGAGLAPVAPGTFGTLLAFP
                     LYWLFFPEVGALEFLLLIVVLFVLGVWACEMTGRALGVSDHGGMVWDETVAFLLVLFF
                     VPGTPYWQAAAFLLFRLFDILKPPPIRYYDRTIKSGFGVMLDDLIAAFYTLLVLAAAK
                     TLLD"
     gene            complement(1676..2479)
                     /locus_tag="AC158Q_11550"
     CDS             complement(1676..2479)
                     /locus_tag="AC158Q_11550"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012995.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase family protein"
                     /protein_id="HIR3340452.1"
                     /translation="MSPDQLDLEAYLKRVGYAGARRPALAVLEELHLAHATHIPFENI
                     DVLLKRPIHLDLPSLQEKLVAGGRGGYCFEQNLLFAAALGKLGFDARLLAAKVRHRGD
                     KDLPRTHMLLLVSVEGAGWLADVGFGGEGLLQPVPFGDGREVQHYAWGYRVIEEKTGQ
                     WLLQSRRQDGWGNLYSFTLDPQTPADCEMANHYTSTHPDSPFVNTLTAQLPTPAARYV
                     LRNRELVTDRGGGNVVRRTLRDDDELLEVLAGTFGLHFPAGTRFAYRDA"
     gene            complement(2577..3773)
                     /locus_tag="AC158Q_11555"
     CDS             complement(2577..3773)
                     /locus_tag="AC158Q_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3340453.1"
                     /translation="MNPVSSNLPILIIGGGIGGLSVALALARKGRRVRVLEKAPEFGE
                     IGYGIQMGPNVSRMLDRLGILKALEPHAVFPDALILVDALDNRELTRIALGKAFIERY
                     HYRYFVIHRRDLHGTILDACRQHEEITLEASRGMTEFEERAGSVIVRCENGAEYEGAA
                     LIGADGLWSPTRAAIVADAAPRVSGHVSYRGVVPTGEIIDRSHLDSMTIWVGPDLHLV
                     QYRLRGGAVMNNVATVSSRRFRRGEKDFGGLDELDEVFSRTDPRVRDMLRYISRDRNW
                     VLHDREPVTNWTRGRATLLGDAAHPTLQYLAQGANMAVEDGVVLAEKVAAAGEDYNRA
                     FLAYQRERMNRTARVVLSSRFFGAYFHVDSGTRELRNELARGRDPENTWEIDWLYRGI
                     EVDGTL"
     gene            complement(3954..4919)
                     /gene="thiL"
                     /locus_tag="AC158Q_11560"
     CDS             complement(3954..4919)
                     /gene="thiL"
                     /locus_tag="AC158Q_11560"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HIR3340454.1"
                     /translation="MALSEFEIIRRFFTHPARHAVLGVGDDAAIVRARRGAQLVVAAD
                     MLVEGRHFSRGARPEQLGHKALAVNLSDMAAMGAVPRWATLTIALPGVDARWLGSFMR
                     GFMRLARRHGMDLVGGDTTRGPLNISVQIIGEVPAGRALRRDGARAGDDVWVSGALGD
                     AALALAAVKRRITLTRGERARLEEKLHAPVPRVALGKTLRGIARSAIDVSDGLVADLG
                     HICERSRVSASVQLDRIPASAVLKRHLARPAARAALLAGGDDYELVFTAGRAKRKAID
                     RLARRLRLKLTRIGKIVRRRGTPRVTVLDGSGRPVALGQTGYQHF"
     gene            complement(5016..5360)
                     /locus_tag="AC158Q_11565"
     CDS             complement(5016..5360)
                     /locus_tag="AC158Q_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340455.1"
                     /translation="MELRDRLDRIEPVSVYHSPFTIHHSRFTGFMMDDRIRKRILIFY
                     FAGFLNLVLGLYVLLNGRALVEQGTFYLLMAFFFGFAAVDFWFAHNLKKKWAEAQTRL
                     DAQGAAAKAGDK"
     gene            complement(5371..5802)
                     /gene="nusB"
                     /locus_tag="AC158Q_11570"
     CDS             complement(5371..5802)
                     /gene="nusB"
                     /locus_tag="AC158Q_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR3340456.1"
                     /translation="MKTDRRRSREFALQGLYQWQLAKGDPAAIAKQLSEAKGFDKIDA
                     DYFTMLLEGAIAAAPELERAIAPHLDREYKRLSPVERAILLLAGYELAHRPDVPFRAV
                     INEAVELAKSYGGTDGYKFVNGVLDKMAAQLRRVEKGVNSD"
     gene            complement(5975..6451)
                     /gene="ribH"
                     /locus_tag="AC158Q_11575"
     CDS             complement(5975..6451)
                     /gene="ribH"
                     /locus_tag="AC158Q_11575"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HIR3340457.1"
                     /translation="MSADEIERIEPDLDGEGLRIGIVVSRFNEEVGEGLLAACTATLV
                     KHGVHPADIRVITVPGALEAPLALQKLANSSHYDALIALGAVIRGETYHFEIVANESA
                     SGITAVALDNGVPVANGILTTENDEQALERVAQKGADCALAAIEMANLMGNLDEQD"
BASE COUNT         1218 a         2197 c         1981 g         1193 t
ORIGIN      
        1 tgatcgacct gctgggggag gattgatcag ccaagggatg aggaatgagg gatgaggaat
       61 gagggatgag ggatgaggga tgacgaataa accgaaaaca ctttttcccc cactctccac
      121 ctaataccgt ccgagattgg gttccggcag ggcacgggtc gagggtctga ccctggacga
      181 gatctcttct tcagctcgcg catcgcacgt ttgaccttct tgcccgccat cttgccgtat
      241 ctcgccatgc cggttgctcc tcacggttgt ctgccatgaa ccgttagagc cgcccgccgc
      301 aacttggttt ttagcgcgga gctgggcggc attcaccgac gtcattctgc cagcatcccc
      361 agcacgcccc ggagcgcatg ctcgaccgac cggcgccgga ccgcctcccg gttgccggcg
      421 aatcgcttgg tctctacgcg cgacctgccg cgcttcatgc cccatgcaaa gcagacggtc
      481 cccaccggct tttccggggt gccgccgccc ggccccgcga cgccacttac cgccaccgcg
      541 acctgcgcgt ggctgcgcgc cagcgcgcct tcggccattt cgcgcacgac ctcctccgcc
      601 accgcgccat gcttgccgag cgttgcatcc ttcacgccga gcatttcgcg cttggaaatg
      661 taggtgtagg tgacgaagcc gcgctcgaac cactccgagc tgcccggcac catcgtgatc
      721 gcttcggcaa tccagccgcc ggtgcatgat tcggcggtgg cgagcatcaa tcggcgcgcc
      781 atgagggccg caccgacttt ctccgccagc ctgaacagcg tggtcggtga ccggtgaccg
      841 gtgaccggtt tcgccttacg gttcttcttc ctcatccctc atccctccgc cctcatccct
      901 cagtcgagca gcgtcttggc cgccgccagc accagcaatg tataaaatgc agcaatgagg
      961 tcgtccagca tgacgccgaa gccgctcttg atcgtccggt cgtagtaacg gatcggcggc
     1021 ggcttgagaa tatcaaacag gcggaacaac agaaacgccg ccgcctgcca ataaggcgtt
     1081 cccggaacga aaaacagcac cagcaggaaa gccaccgtct cgtcccacac catgccgccg
     1141 tggtcggaga cgccgagcgc gcggccggtc atctcgcagg cccagacgcc caggacgaac
     1201 agcaccacga tcagcagcag gaactcgagc gcgccgactt ccgggaagaa caaccagtag
     1261 agcggaaaag ccagcagcgt gccgaacgtc cccggggcga cgggcgcgag cccggcaccg
     1321 aatccgaacg cgaccagata ggcgggacgg ctcaacacga agcgccacgt cggccgcagg
     1381 accaggtcag acatgcaaaa ccgtccaacg aaaccgaaaa taaatgcaga tacgcgcaga
     1441 tatcaagatg aaaacgcctc cctgactacc catcttccgc caccccggcg ccacgcgcca
     1501 cccggctggc ccgcttcgct gaatcgattc gggtgtgcgc aggcaagttc ttcgaaaccc
     1561 cggcccccct cggacaggag gcgagcggta tccgcccatc acggagaatt acccaattgc
     1621 cgcatatttt gaatcctttg tgtcccttgt gtcgttcgtg tcgaggcttt gagcatcacg
     1681 cgtcacggta agcaaaccgt gtccccgccg ggaaatgcag cccgaacgta ccggcgagga
     1741 cttcgagcag ctcgtcgtca tccctgagag tgcgccgcac gacgttgccg cccccgcggt
     1801 cggtgacgag ctcgcggttc ctgagtacat agcgcgccgc cggtgtcggc aactgcgccg
     1861 tcagcgtgtt cacgaacggc gagtccgggt gcgtggaggt gtaatggttc gccatctcgc
     1921 aatcggctgg cgtctgcggg tccagggtaa acgagtagag gttgccccac ccgtcctgcc
     1981 gcctcgattg aagcagccac tgcccggtct tctcctcaat gacgcgatag ccccaggcat
     2041 agtgctgtac ttcgcgtccg tcgccgaacg gcaccggctg cagcagtccc tccccgccga
     2101 agccgacgtc cgccagccag ccggcgccct ccacgctgac cagcagcagc atgtgggtgc
     2161 gcggcaggtc cttgtcgccc cggtggcgga ctttcgcagc gagcagtctc gcgtcgaagc
     2221 ccagcttgcc gagcgccgcc gcgaacagca ggttctgctc gaagcagtaa ccgccgcgac
     2281 cgcccgcgac aagcttttcc tgcaggctgg gaagatcgag gtggatagga cgcttcagca
     2341 gcacatcgat attttcgaac gggatgtgcg tcgcgtgcgc gaggtgcaac tcttccagca
     2401 cggccagcgc cggccgccgc gccccggcat acccaacgcg cttcaggtac gcctccagat
     2461 caagctgatc gggactcatc agcggctcat gattccggtg acgaagtgat gaagtgacgc
     2521 agtgacggtt attgctttgt gaaatcggcg tgtatcggcg ttcatcggcg gctgcattaa
     2581 agcgttccgt ccacttcgat cccgcggtag agccagtcga tttcccaggt gttctccggg
     2641 tcgcggcccc gcgcaagctc gttcctaagc tcgcgcgtgc cggaatcgac gtggaaatag
     2701 gcgccgaaaa agcgcgagga gagcaccacg cgcgcggtgc ggttcatccg ctcgcgctgg
     2761 taggcgagga aggcgcggtt gtaatcctcc cccgccgcgg cgaccttctc ggccagcacg
     2821 acgccgtcct cgaccgccat gttcgccccc tgcgccaggt actgcagcgt cgggtgcgcc
     2881 gcatcgccca gcagcgtcgc ccggccgcgc gtccagttgg tcaccggctc gcggtcgtgc
     2941 agcacccagt tccggtcgcg cgagatgtag cgcaacatgt cgcgcaccct gggatcggtg
     3001 cgcgagaaca cctcgtcgag ctcgtccagg ccgccgaagt ccttctcgcc gcgccggaaa
     3061 cgccggctgg agacggtcgc gacgttgttc atcaccgctc cgccgcgcaa acgatactgc
     3121 accaggtgca gatcggggcc cacccagatc gtcatcgaat cgaggtggct gcggtcgatg
     3181 atttcgccgg tcggcaccac cccgcgatag gacacgtggc ccgacacgcg cggcgctgcg
     3241 tcggccacga tggccgcgcg cgtgggcgac cacaggccgt ccgcgccaat gagcgcggca
     3301 ccttcgtatt cggcgccgtt ctcgcagcgc acgatcaccg agcctgcacg ctcctcgaat
     3361 tccgtcatgc cgcgcgaagc ttccagcgtg atttcctcgt gctgcctgca cgcgtcgagt
     3421 atggttccat ggagatcgcg ccggtggatg acgaagtagc ggtaatggta gcgctcaata
     3481 aatgccttgc cgagcgctat gcgcgtgagc tcccggttgt cgagagcatc gaccaggatg
     3541 agcgcatccg gaaagactgc gtgcggctcc agcgccttga ggatacccag gcggtcgagc
     3601 atgcggctca cgttcggccc catctggatg ccgtagccga tctcgccgaa ctccggcgcc
     3661 ttctccagca cgcgcacgcg ccggcccttc cgcgccagcg cgagcgccac cgacagcccg
     3721 ccgatgccgc cgccgatgat gaggatagga aggttggaag agacaggatt cacagaaatt
     3781 caatcaggaa aaccgagcac ccgccaacac cgtaaataga caggattaac aagattaaca
     3841 ggattcaaac tacaaacttc tttcgccttg tgtgtcttaa tcttgtaaat cctgttaatc
     3901 ctgtctattt gagatcttga cgggttttcg aatccaactg ttaatcctgt ctattagaaa
     3961 tgctggtagc cagtctggcc gagcgcgacc ggtcggccac tgccatcgag cacggtcacg
     4021 cgcggggtgc cgcgccgccg cacgatttta ccgatgcggg tcagcttaag gcgcaggcgc
     4081 cgcgcaaggc ggtcgatcgc cttccgcttc gcgcgtccgg cggtaaaaac cagctcgtag
     4141 tcgtcgccgc cggcaagcag ggcggcgcgc gcggcgggac gggcaagatg tcgcttcagc
     4201 accgccgaag ccggaatccg gtcgagttgc acgctcgccg agacccgcga gcgctcgcag
     4261 atgtggccga gatcggcgac gaggccgtcg gagacgtcga tggcgctgcg cgcgatgcca
     4321 cgcaaggtct tgcccagggc gacgcgcggc acgggcgcat gcagcttttc ttcgagccgc
     4381 gcgcgttccc cgcgcgtgag cgtgattctc cgtttcaccg cggccagcgc cagggcggcg
     4441 tcgccgagcg cgccggaaac ccagacatcg tcgcccgcgc gggcgccgtc gcggcgcaat
     4501 gcgcgcccgg cggggacttc accgatgatt tgcacgctga tgttgagcgg gccgcgcgtc
     4561 gtgtcgccac cgacgaggtc catgccgtgg cgccgcgcaa gccgcatgaa accgcgcatg
     4621 aaggaaccca gccagcgcgc atccacgccg ggcagcgcaa tggtcagggt cgcccagcgc
     4681 gggacggcgc ccatcgccgc catgtcggac aggttgaccg caagcgcctt gtggcccaat
     4741 tgctccggtc gggcgccgcg ggaaaaatgc cgcccctcga ccagcatgtc cgccgcaacg
     4801 acgagttgcg cgccgcgccg cgcccgcaca attgcggcat cgtcgcccac gcccagcacc
     4861 gcgtggcgcg cggggtgggt gaaaaaccgc ctgatgatct cgaactcgga cagcgccatc
     4921 ggccggatgc gtgataagtg acgggtgata ggtgataggg ctctatgcca gccgtgcgaa
     4981 gcacggaacc cttatcattt atcacttatc actcatcact tatcacctgc tttagcagcc
     5041 gccccctgcg cgtcaagcct tgtctgggcc tcggcccact tcttcttcaa attatgcgcg
     5101 aaccagaaat ccaccgccgc gaaaccgaag aaaaaggcca tcagcaggta aaacgttccc
     5161 tgctcgacca gggcccggcc gttcagcagc acgtagagcc ccagaaccag gttgagaaat
     5221 cccgcgaagt agaaaatcag tatccgtttc ctgatcctgt cgtccatcat gaaccccgtg
     5281 aatcgtgaat gatgaatggt gaatggtgaa tggtaaacag aaacaggctc tattcgatcg
     5341 aggcggtcgc gaagctccat tcactactcg ctaatcacta tttacgccct tttcaacgcg
     5401 gcgaagctgc gccgccatct tgtcgaggac gccgttcacg aacttgtagc cgtccgtgcc
     5461 gccgtagctc ttcgccagct ccaccgcctc gttgatcacc gcccggaacg gcacgtcggg
     5521 ccggtgcgcg agctcgtagc cggcgagcag caggatagcg cgctcgaccg ggctcaagcg
     5581 cttgtattcg cggtcgaggt ggggggcgat cgcccgttcc agttcgggtg ccgcggctat
     5641 cgcgccttcc agcagcatcg tgaaatagtc cgcgtcgatc ttgtcgaagc ccttcgcctc
     5701 ggacagctgt ttcgcgatcg cggccgggtc gcccttcgcc agctgccact ggtacaggcc
     5761 ctgcagggcg aactcgcgcg agcggcgtct gtcggttttc atgttcgcgt aataagacag
     5821 gatttacaga aaggctcaag aacccgtcaa aagcttcaat agacaggatt aacaggattt
     5881 acaagattaa gatacataaa cctaaagaag gtcgtagttt gaatcctgtt aatcttgtta
     5941 atcctgtcta ttgttggggt taacaggttt tcagttaatc ctgttcgtcc aggttgccca
     6001 tcaggttggc catctcgatt gcggccagcg cacagtcggc gcccttctgc gccacccgct
     6061 cgagcgcctg ctcgtcgttt tccgtcgtca ggatcccgtt cgcgaccggc acgccgttgt
     6121 cgagcgcaac cgccgtgatg ccgctcgcgg attcgttggc gacgatctcg aagtggtaag
     6181 tctcgccgcg tatcaccgcg ccgagcgcga tcagcgcgtc gtaatggctg ctgtttgcga
     6241 gtttctgaag ggcaagcggc gcctccagcg cgcccggaac ggtaatgacg cggatgtcgg
     6301 ccggatgcac gccgtgcttg acgagcgtcg cggtgcacgc cgcgagcagc ccctcgccca
     6361 cctcctcgtt gaagcggctg accacgatgc cgatgcgcag cccctctccg tccaggtctg
     6421 gctcgattcg ctcgatttca tctgcgctca ttggtcgttg ctcgttgctc gttgctcgtt
     6481 tttcgttgct cgttgcccgt caccgcgtca cttgtctttt gccggcggtt gcaggtaccc
     6541 gacgacctcc accccgaaca ccgccatgct gggcatccgg cgcgggagc
//
LOCUS       DBNGSG010000445         6404 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4560004, whole genome shotgun sequence.
ACCESSION   DBNGSG010000445 DBNGSG010000000
VERSION     DBNGSG010000445.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6404)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6404)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6404
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4560004"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..1048)
                     /locus_tag="AC158Q_11580"
     CDS             complement(77..1048)
                     /locus_tag="AC158Q_11580"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3340458.1"
                     /translation="MRSSRYGISFFLFPFLFSLGYGCAIAQPYPSKPVRVIAPYPAGG
                     PVDAIARILAQNLALGQNVIVENRSGAGGSVGAEVVARSSPDGYTMLIGNTGPMTINP
                     VLRQDLPYDPKKDFAPVSWLTSAQMVLMVHPSLPVHSVREVVALARANPGALNYGSAG
                     IGNLTHLGMELFQSMAKVKMIHVPYKGVAPAYVDLMSGQIAVMFGNVSGPLAHIRAGR
                     LRAIAVTSSKPSPVLPGVPRVADVYPGFDLVTWMGIFLPGGTPEAIRSRLQGEYIKTL
                     QAKDVSERLVALGNDVVAGSGADLAALLDRELKLYGKIVKAAGIKAQ"
     gene            complement(1167..2171)
                     /locus_tag="AC158Q_11585"
     CDS             complement(1167..2171)
                     /locus_tag="AC158Q_11585"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR3340459.1"
                     /translation="MAAKLRIKDTRDRKNRKLTRKDVHKAAKRYRNWGRWGKDDEIGT
                     LNFTTASDIVAAARLVRKGKVISLALNFDERGPQGGKTKYPALGRFNPIHVMTRTGTD
                     AYAGVLDHRKIRGTDDIVIMGLQSGTQWDGLGHILYYDHMWNGYDCRNVTSGGAQKGG
                     IEKTREKMVGRGVLLDVARALGKKWLPDGCAITNEMLDFTEKKLGVRVRRGDFLMVRT
                     GHIERCLASKSWDGYPGGDAPGFAFETVDWLYKREIAGVATDTWGAEVRPNESEDTNQ
                     PWHWITIPIMGLTVGEIFDFAALAKDCAEDKRYEFLFVAPALPITGGVGSPVNPLAIK
                     "
     gene            2407..3267
                     /locus_tag="AC158Q_11590"
     CDS             2407..3267
                     /locus_tag="AC158Q_11590"
                     /EC_number="3.5.1.68"
                     /inference="COORDINATES: protein motif:HMM:NF016873.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050129 - N-formylglutamate deformylase
                     activity [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HIR3340460.1"
                     /translation="MIHAEPGLFLRYEPTAAPAPLLVDVSRSGREYPKEYRSPLPFTT
                     VHDNVSMHVEDLWAGTPAAGGTLLYCCFPNTWVDVNRSECDMDPAVVDGRWPRELKPT
                     ARTLEGLGLIKTRSRYGEPFQERKLTVAEIEERLARHYRPYHAELKRCVDTLHQRFGV
                     LRQISCHCMSAVGAPTHPDAGKPRADFCVSDLKGRTASRQSMELVVETLKRYGYSVSV
                     NDPYIGNELIARHGDPARGVDSIQVEINKKLFMDTGTFRRTPGFDRLKADLDRLLAVI
                     VQDSMGRARS"
     gene            complement(3268..4245)
                     /locus_tag="AC158Q_11595"
     CDS             complement(3268..4245)
                     /locus_tag="AC158Q_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3340461.1"
                     /translation="MNATITWRRVFLAGALAACSLHAGAQPYPARPIRLIMPFPPGGA
                     SDLIIRPVAQKLSEQLGQTIVIDNRPGAGGNIAAELAARAAPDGYTLLFAASANFSIN
                     PNLYAKLPYDPVRDFAAVTMLTRLSNLLTVHPSLPAASVSELTALARTQPGKFAYGSA
                     GNGTTLHIAGALYRARTGTNVIHVPYKGGGPAQLDLIGGRLQFMFDSFSPALPQVRAG
                     KLRALAVTTLKRSPALPQVPTMVESGLPDFEISGWFGIAAPAGTPRPVVERLGAELQR
                     VMQLPGIRERLLAQGIEPVGSAPAEFSRQVRSELAKWARIVKESGARVD"
     gene            complement(4253..5236)
                     /locus_tag="AC158Q_11600"
     CDS             complement(4253..5236)
                     /locus_tag="AC158Q_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3340462.1"
                     /translation="MANTVRGGMVLAVLCVAASGLVAAADDAKGYPGRSVRFIVPFPP
                     GGATDVVTRVVAQKLNDQYKRQIAIVDNRGGAGGVLGSELAARAAPDGYTLVMGTTGT
                     HAINASLYAKLSYDPVRDFTAVTPAALLPNLIVAHPSVPAKNVKELVALARARPGQLS
                     YASSGNFLYLSGALFTSMANIKMLHVPFKGGAQAMPAVMSGEVALSFSTIVSALPQVQ
                     AGKLRGLAVTSAQRFATAPAYPTVAESGLPGYEAVAWYGVFAPAGTPRDVVKQLNTDI
                     TRIVNALDVRDLFLKQGAISYATSPEEFSKIVQRDVVKWAKVVKTSGATAD"
     gene            complement(5257..6351)
                     /locus_tag="AC158Q_11605"
     CDS             complement(5257..6351)
                     /locus_tag="AC158Q_11605"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3340463.1"
                     /translation="MERKDLGIAVIGAGRIGGLRARLASEHPAVKFLAVSDRDPARAR
                     DLAQKTGAQFHSGDNAAVIARREVNAVIVSSSEGAHVEPILQAIELGKTVLVEKPIAL
                     SLADGDRIIRAAEKAGVSVRVGYSRRYKERYLIAKEQIEQGRVGRIVGAAARVFNLRS
                     QATAILKRNPDATPVVDALTYYVDLMNWFIGSKGRGAQVKEVFARGQKGVLKESGFDT
                     EDVSYAILTYADGAVVNLGIAYSLPDAYPSRGHAARVEVLGTGGVIILDDDHTDQLMY
                     SEKGIPHVYLPDQPVNMVFLQSGTPGDWALGDFWGPVAAETRAWLDHLAVGKPCALAT
                     AREARATLEVTLAIEHSAATGKPVSLPLAG"
BASE COUNT         1083 a         2172 c         2026 g         1123 t
ORIGIN      
        1 cccgtataca ggtggattcc ctggacgccc gccttcgcgg gagtgacggc gtttttcaac
       61 ggtctgttaa atacctttac tgcgccttaa ttccggctgc cttgacgatc ttcccgtaca
      121 gtttcaactc gcgatcgagt agcgcagcga gatccgcacc gctgccggcg acgacgtcgt
      181 ttccgagcgc gacgagacgc tccgatacgt ccttcgcctg cagggtcttg atgtattccc
      241 cctgcagcct ggaacgtatg gcttccgggg tgccgcccgg caggaagatg cccatccagg
      301 tcacgaggtc gaatccggga tacacgtccg caaccctcgg cacgcccggc aggacgggcg
      361 aaggcttcga cgaggtcacg gcaatggcgc gcagccggcc cgcccggata tgcgccagcg
      421 gaccggaaac gttgccgaac atgacggcga tctggcccga catgaggtcg acataggccg
      481 gcgccacccc cttgtagggg acgtggatca tcttcacctt cgccatcgat tgaaacaact
      541 ccatgccgag atgcgtcagg ttcccgatgc cggccgatcc ataattgagg gcgccgggat
      601 tggcgcgcgc gagcgcgacc acttccctca ccgagtgcac gggcagggac gggtgcacca
      661 tgaggaccat ctgcgcggag gtcagccacg aaacgggcgc aaagtccttt ttcgggtcat
      721 agggcagatc ctgccgcaac acgggattga tggtcatcgg cccggtgttg ccgatcagca
      781 tcgtgtaacc gtcgggcgac gagcgtgcga cgacttccgc gccgacgctg cctccggcgc
      841 cggagcggtt ttccacgatc acgttctggc cgagggcgag gttctgcgcg agtatccgtg
      901 cgatcgcatc aaccggcccc ccggcgggat acggcgcgat cacccgcacc ggcttcgaag
      961 gatacggctg cgcgatcgcg cagccgtatc caagggaaaa gaggaaaggg aaaaggaaaa
     1021 aggagatgcc gtatcgtgac gatcgcataa actcgcctcc tcgtcttgat ttattatgaa
     1081 attaaatcat ctactccagt ttctttcttt cgtacggacc cttttcactt ttcccttgtg
     1141 ccttttccct tgaactccat cgcccatcac ttgatggcga gcggattcac cggcgagcct
     1201 acccccccgg taatcggcag cgcgggcgcg acgaacagga actcgtagcg cttatcctcc
     1261 gcgcagtcct ttgccagcgc cgcgaagtcg aagatctccc cgaccgtgag ccccataatc
     1321 gggatcgtga tccagtgcca gggctggttg gtgtcctcgc tctcgtttgg ccgcacctcc
     1381 gcgccccagg tgtcagtggc gacgcctgcg atttcgcgct tgtacaacca gtccacggtc
     1441 tcgaacgcga accccggcgc gtcgccgccc gggtagccgt cccagctctt cgaggcgagg
     1501 cagcgctcga tatggccggt gcgcaccatc aggaagtcac cgcgccgcac ccgcacgccc
     1561 agcttcttct cggtgaaatc gagcatctcg ttggtgatcg cgcagccgtc gggcagccat
     1621 ttcttgccca gcgcgcgcgc gacatcgagc agcaccccgc gccccaccat cttttcccgc
     1681 gtcttctcga tgccgccttt ctgcgcgccg ccggaagtca cgttgcggca gtcgtacccg
     1741 ttccacatgt gatcgtaata aaggatgtgg ccgagtccgt cccattgcgt gccggactgc
     1801 agccccatga tgacgatgtc gtcggtgccg cggatcttgc ggtggtcgag cacgccggcg
     1861 tacgcatcgg tgccggtgcg cgtcatcacg tggatcgggt tgaagcggcc gagcgccggg
     1921 tacttggtct tgccgccctg cgggccgcgc tcgtcgaagt tcaatgcgag cgagatgacc
     1981 ttgcccttcc tcaccaggcg cgccgcagcc acgatgtcgg acgcggtggt gaagttgagc
     2041 gtgccgatct cgtcgtcctt gccccagcgg ccccagttcc ggtagcgctt cgccgccttg
     2101 tgcacgtcct tacgcgtgag cttgcggttt ttcctgtcgc gcgtgtcctt gattctcaac
     2161 ttcgctgcca tgtggagctt ccttcttttc ggagtagaga gaggcttgca gtctatccag
     2221 cgttgcagga ctgcgtcaac gaaatctctt ttagattccc ctcctgcccg aggaggggaa
     2281 tcttccaacg ccacggcgct ccgtgccacc ctctcaggca gaagggaagt cgttctcggc
     2341 ccattgactt tgcgcggggc ttcgcctaga aatcgccagt cgcccgacga gaggaagtgc
     2401 accgcgatga tccatgccga gcccggcctg ttcctgcgct acgagccgac cgccgcgccg
     2461 gcgccgctgc tcgtggacgt ctcgcgcagc ggccgcgagt accccaagga gtaccgctcg
     2521 ccgctgccgt tcacgaccgt gcacgacaac gtctcgatgc atgtcgagga cctgtgggcc
     2581 ggcacgccgg cggcgggcgg caccttgctc tactgctgct tccccaacac ctgggtggac
     2641 gtcaaccgca gcgaatgcga catggacccg gcggtggtgg acggccgctg gccccgggaa
     2701 ttgaagccca ccgcgcgcac gctcgagggg ctgggcctca tcaagacccg gtcgcgctac
     2761 ggcgagccgt tccaggagcg caagctcacc gtcgcggaga tcgaggagcg cctggctcgc
     2821 cactaccgtc cttaccatgc cgaactgaag cgctgtgtcg acacattgca ccagcgtttc
     2881 ggcgtgctgc gccagatttc ctgccattgc atgtcggcgg tcggcgcgcc cacccatccc
     2941 gacgccggca agccgcgcgc ggatttttgc gtgagcgacc tgaagggcag gaccgcctcc
     3001 aggcaatcaa tggagctggt ggtggagacc ctgaagcgct acggctacag cgtgtcggtg
     3061 aacgatccct acatcggcaa cgaactgatc gcccgccacg gcgaccccgc gcgcggagtg
     3121 gattcgatcc aggtcgagat caacaagaaa ctcttcatgg acaccgggac tttccgcagg
     3181 acgcccggct tcgaccggct caaggccgac ctcgatcgcc tgctggctgt catcgtgcag
     3241 gacagcatgg gccgcgcgcg aagctgatca gtccacgcgc gcgccggatt ccttgacgat
     3301 gcgggcccac ttcgcaagct cgctgcggac ctgcctgctg aactcggcgg gggcgctgcc
     3361 gacgggctcg atgccctgcg ccagcaaccg ctcgcgtatt cccggcaact gcatcacgcg
     3421 ctgaagctcg gcgcccaggc gctcgaccac gggccggggc gtgcccgcgg gcgccgcaat
     3481 gccgaaccac cccgatatct cgaaatccgg caggccggat tccaccatcg tcggcacctg
     3541 cggcagcgcc ggcgagcgct tgagcgtggt caccgcgagc gcgcgcaatt tgcccgcccg
     3601 cacttgaggc agcgccggcg aaaacgagtc gaacatgaat tgaagccggc cgccgatcag
     3661 atccagttgc gcggggccgc cgcccttgta cgggacgtgg atcacgttag tgcccgtgcg
     3721 cgcgcggtag agcgctcccg cgatatgcag cgtcgttccg ttgcccgcgg agccgtaggc
     3781 gaatttcccc ggctgcgtcc tggcgagggc ggtcaattcg gacaccgaag ccgcgggcag
     3841 cgaggggtgc accgtgagca ggttggagag ccgggtcagc atcgtgaccg cagcgaaatc
     3901 gcgcaccggg tcgtacggca gcttcgcgta caggttcgga ttgatcgaga aattcgcgct
     3961 cgccgcgaac aacagcgtgt agccgtccgg cgccgcccgg gcggccagct cggcggcaat
     4021 gttgcctccg gcgccgggcc ggttgtcgat cacgatagtc tgcccgagct gctccgagag
     4081 tttctgcgcg accggacgaa tgatgaggtc agacgccccg cccgggggaa acggcatgat
     4141 cagccggatc ggccgcgccg ggtacggctg cgccccggcg tgcaggctac acgccgccag
     4201 cgctcccgca agaaacacgc gccgccaggt gatggtcgca ttcatgaagt aatcaatccg
     4261 ccgtcgcgcc cgaggttttg acgaccttcg cccacttcac cacgtcgcgc tgcacgatct
     4321 tcgagaattc ctcggggctg gtggcgtagg aaatcgcgcc ctgcttgagg aacaggtcgc
     4381 gaacgtccag cgcattgacg atccgggtga tgtcggtgtt caactgcttc accacgtcgc
     4441 gcggcgtgcc ggcgggcgcg aaaacgccgt accacgccac cgcctcgtaa cccggcaggc
     4501 cggactcggc caccgtcgga taggcgggcg cggtggcgaa ccgctgcgcg ctggtcaccg
     4561 ccaggccgcg cagtttcccc gcctgtacct gcggcagcgc cgagacgatg gtcgagaacg
     4621 acagcgccac ctctcccgac atcaccgccg gcatcgcctg cgcgccgccc ttgaatggaa
     4681 cgtgcagcat cttgatgttc gccatgctgg taaacagggc gccgctcaag taaagaaagt
     4741 ttcccgatga agcgtaagag agctgcccgg gccgcgcgcg tgccagcgcg acaagctcct
     4801 tcacgttctt cgccggcacc gacgggtgcg cgacgatcag gttgggcagc agcgcggcag
     4861 gcgtcacagc ggtaaagtcc cgcaccgggt cgtacgacag cttggcgtag aggctggcgt
     4921 tgatcgcgtg agtgccggtc gtgcccatca ccagcgtgta gccgtcgggc gccgcgcgcg
     4981 ccgcgagttc cgagcccagc acgccgcctg cgccgccgcg attgtcgacg atggcgatct
     5041 gccgcttgta ctggtcgttc agcttctgcg ccacgacgcg cgtgacgacg tcggtcgccc
     5101 cgcccggcgg gaacggcacg atgaagcgca cggaacggcc gggatagccc ttcgcatcgt
     5161 ccgccgccgc aaccagcccg gatgcggcaa cgcacagcac cgccaggacc atcccgccgc
     5221 gcacggtgtt tgccatgact tggatcttcg ggctcatcat cccgccaacg gcaagctgac
     5281 cggcttgccc gtcgccgccg aatgctcgat cgcgagcgtc acttcgagtg tcgcgcgtgc
     5341 ttcacgcgcc gtagccagtg cgcacggttt gccgaccgcg agatgatcga gccaggcgcg
     5401 cgtttcggcc gcgaccggcc cccagaagtc gcccagcgcc cagtcacccg gagtgccgct
     5461 ctgcaggaac accatgttga ccggctggtc ggggagatag acgtgcggga tgcccttctc
     5521 ggagtacatc aactggtcgg tgtggtcatc gtcgaggatg atcacgccgc cggtgccgag
     5581 gacttcgacc cgcgccgcat gaccgcgtga cggatacgca tccggcaggg aataagcgat
     5641 gccgagattg accaccgcgc cgtcggcata ggtgaggatc gcgtagctca cgtcctcggt
     5701 gtcgaacccg gattccttga gaaccccctt ctgcccgcgc gcgaagacct ccttcacctg
     5761 cgcgccccgc cccttgctgc cgatgaacca gttcatgagg tcgacgtaat aggtcagcgc
     5821 atccaccacc ggcgtcgcgt ccgggttgcg cttgagtatt gcggtcgcct gcgagcgcag
     5881 gttgaagacg cgcgcggcag cgccgacgat ccgcccgacg cgcccctgct cgatctgctc
     5941 cttggcgatg aggtagcgtt ccttgtagcg gcggctgtag ccgacgcgca cactgacgcc
     6001 ggccttctca gcagcgcgaa tgatccggtc gccgtccgcg aggctgagcg cgatcggctt
     6061 ctcgacgagc acggtcttgc cgagttcgat cgcctgtagg atcggctcga cgtgcgcccc
     6121 ctcgctggaa gagacgatga cggcgttgac ctcgcggcgc gcaatgacgg cagcattgtc
     6181 gcccgaatga aactgcgcgc cggttttctg cgcgagatcg cgcgctcgcg ccgggtccct
     6241 gtcggatacc gcgaggaact tgactgcagg atgctccgag gcaaggcgtg cacgcagccc
     6301 cccgatgcgg ccggcgccaa tgactgcaat gcctaaatcc ttgcgttcga tggtcctctc
     6361 ccggcaataa gcggcaaacg cagagcggtc gtctcaacca tttg
//
LOCUS       DBNGSG010000446         3779 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3711232, whole genome shotgun sequence.
ACCESSION   DBNGSG010000446 DBNGSG010000000
VERSION     DBNGSG010000446.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3711232"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3730
                     /locus_tag="AC158Q_11610"
     CDS             <1..3730
                     /locus_tag="AC158Q_11610"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR3340464.1"
                     /translation="LRVRNLLRLKTLGDFFRNHGSILEQQVKEQLEAVRVSEVSYRRL
                     FEAAQDGILILDFETGRIDDVNPYLARLLGFSPGEMIGKTVGELSPFRDIESNQVMLE
                     RLQKDGYVRYDDLPLETRDGRRVAVEFVSNVYQADGKKVIQCNIRDITERRNAEKSLK
                     ELGAKYHTVFELSSDAIMLFSAKGFFDCNLAALRLFACPARADFINKHPAELSPPIQP
                     GGEDSMRLADQHIAAAIENGSKRFEWTHRRLDGTEFLAEVLLTAMKLDGKEVLQAVVR
                     DFTERKKAESRIAYLNRVYAVLSGINTLIVRARDRDQLYTEACRIAVEQGGFRMSLLA
                     IVDHRTMKVVPVASAGKDEALLSAIKTLLSSSEQAPHTMVACAIREKQAVVSNDSQSD
                     PRVLIRDKYAESGIRSMAILPLIVSDKAVGVLALYASEREFFHAEEMALLTELARDIA
                     FAIDHIGIEERIARLSRIRAVTGGINALIVRARNRQELFEGAARIVVEQGHFEMAWIG
                     ALDPATQDVTPVAWAGESAEELTRAKSSARDDMLQGRGAVGQAIRAQRPVFNNDITNQ
                     SFGGPRVKEILRLGFQSQITLPLFDDQNVAGTLTMYAREPDFFDAEEVRLLTDLAGDI
                     SFALRNISRQQKLDKLARIRAISSEINASIIRIHDRDALLRETCRIAVEHGKFELVWI
                     GTVDLPKQEIRPIAWSGFSPEVAHGVSWKSSRIAGGTLGEAIRTAKPTFRNDIETGMT
                     AGGMRAAALKQGYFSTACLPFVVDGNVVALISLFAAGKGFFDADELALLNEVASDVSF
                     ALQSIEKQKKLEYLSYYDVLTGLANRTLFLERVSQHLRSAATGGHKLAVFLIDLERFK
                     NINDSLGRPAGDALLLQVAEWLTRKAGDASLLARVGADHFAAVLPQVNRDGDMAHLLE
                     NWMEVFLGHPFRLNDAVFRVALKVGAALYPDDGADAEILFRNAEAALKKAKAGGDRYL
                     FHTQKMTETVAGTLTLENQLRQALDNDEFVLHYQPKVNLVSGRMTGAEALIRWNDPRT
                     GLVPPGQFIPILEETGLIHEVGRWALRKAVEDYLRWRAAGLSVVRIAVNVSPLQLRNR
                     SFVAEVREAIGIDAQSAAGLELEITESLIMEDVKHSIVTLQAIRALGVTVAIDDFGTG
                     FSSLSYLARLPVDTLKIDHSFVTDMIAGPEGLALVSTIINLAHSLRLKVVAEGVETEE
                     QSRLLRLLRCDEMQGFVFSKPVPGEVFEARFLALPAAA"
BASE COUNT          752 a         1133 c         1224 g          670 t
ORIGIN      
        1 gctgcgggtg cgcaatctat tgcgcctgaa aaccctgggc gattttttca ggaaccatgg
       61 ctcgatcctg gagcagcagg taaaagaaca gctggaggcg gttcgcgtct ccgaagtcag
      121 ttatcgccgg ctgttcgagg cggcgcagga tggcatcctg attctggact tcgagacggg
      181 gcgcatcgac gatgtgaacc cctatctcgc caggctgctg gggttttccc ccggggaaat
      241 gatcggcaag accgtcggcg aactcagccc gttcagggac atcgagtcga accaggtcat
      301 gctcgagcga ctgcagaagg acgggtacgt ccgttacgac gacctgccgc tggaaacgag
      361 ggatggccgc cgcgttgcgg tggagtttgt cagcaacgtg taccaggcgg acggcaagaa
      421 agtgattcaa tgcaacatcc gcgacatcac cgaacgcagg aacgcggaaa aatcgctgaa
      481 ggaactgggt gcgaaatacc ataccgtgtt cgaactgtcg tccgacgcaa tcatgctgtt
      541 cagcgcgaag ggctttttcg attgcaacct ggccgccctg cggctgttcg cctgccccgc
      601 gcgcgccgat ttcatcaaca agcatcccgc cgagctctcc ccgcccatcc agcccggcgg
      661 ggaggattcg atgcgcctgg ccgatcaaca catcgccgcg gccattgaaa acggcagcaa
      721 gcggttcgaa tggacgcatc gccggctcga cggcaccgag tttctggcag aggtattgct
      781 gacggccatg aaactcgacg gcaaggaggt gctgcaagcc gtggtgcgcg acttcaccga
      841 gcgcaagaaa gcggagagcc ggatcgcgta cctcaaccgg gtctatgccg tgttgagcgg
      901 catcaatacg ctgatcgtgc gcgcgcgcga tcgggatcag ctttacacgg aggcgtgccg
      961 catcgcggtg gagcaaggcg gattccgcat gtccctgtta gccatcgtgg atcaccgcac
     1021 gatgaaggtg gttccggtcg cctcggcggg caaggacgag gcgcttctgt ccgccatcaa
     1081 aacgcttcta tcgtcgagcg agcaggcgcc ccataccatg gttgcgtgcg cgatccggga
     1141 gaagcaggcg gtcgtgtcca acgattcgca gagcgacccc agagtcctga ttcgtgacaa
     1201 atatgccgaa tccggcatcc gctcgatggc aatcctgccg ctgatcgtat cggacaaggc
     1261 ggtaggcgtg ctcgcgttgt acgccagcga gcgcgaattc ttccatgcgg aagagatggc
     1321 gctgttgacc gaactggctc gcgatatcgc ttttgcgatc gaccatatcg gcattgagga
     1381 gaggatcgcc cgcctgagcc gcatccgtgc ggttacgggc ggtatcaatg cgctcatcgt
     1441 gcgggcacgc aatcgccagg agcttttcga gggcgcggcc cgcatcgtcg tcgagcaggg
     1501 ccatttcgaa atggcctgga tcggcgcgct cgatccggcg acgcaggacg tgacgcccgt
     1561 tgcctgggcc ggcgaaagcg cagaagagct cacccgggcc aaatcttccg ccagggacga
     1621 tatgctgcag ggaagaggag cggtcggaca ggccatccgg gcgcagcgcc cggtattcaa
     1681 taacgacatc accaaccaaa gttttggcgg accgcgcgtg aaggaaatac tgcggctggg
     1741 cttccagtcg cagatcacgc tgcccttgtt cgatgaccag aatgtcgcgg ggactctcac
     1801 gatgtatgcc agggagccgg acttctttga cgccgaagaa gtccggttgc tgaccgatct
     1861 tgccggtgac atctcatttg ccctcaggaa tatcagcagg cagcagaagc tcgacaagct
     1921 ggcgcgcatt cgcgcgatat ccagcgagat caacgcatcc atcatccgca tccatgaccg
     1981 cgatgcgctg ctgcgcgaaa cctgccgcat cgccgtcgag cacgggaagt tcgagctggt
     2041 atggatcgga acggtcgact tgccgaagca ggagatccgg ccgattgcct ggagcggttt
     2101 ctcgccggag gtcgcacatg gggtgagctg gaagagctcc cgcatcgcgg gcgggaccct
     2161 gggcgaggcg atccgcaccg cgaagccgac gttccgcaac gacatcgaga ctggaatgac
     2221 ggccgggggc atgcgggccg cggcgttgaa gcaaggctat ttttccacgg cctgcctgcc
     2281 attcgtggtg gacggcaatg tggtcgccct catttccctt ttcgcggcgg gaaaagggtt
     2341 ctttgacgcc gacgagctgg cgcttctcaa tgaagtggca tccgacgtct cgtttgcgct
     2401 gcagtccatc gagaaacaga aaaagctgga atacctctcg tactacgatg tgctgaccgg
     2461 tctcgcgaat cgaaccctgt ttctcgagcg ggtgtcgcag cacctgcgca gcgccgccac
     2521 cggcgggcac aagctggccg tgttcctgat cgacctggag cggttcaaga acatcaacga
     2581 cagtctcggc cggccggccg gcgacgcgct cctgctgcag gtggcggaat ggttgacgcg
     2641 caaggcaggc gacgccagcc tgctggcacg agtgggggcg gatcatttcg ccgcggtgct
     2701 gccgcaagtc aatcgggacg gcgacatggc gcatcttctc gagaactgga tggaagtgtt
     2761 tctggggcac ccgtttcgcc tgaatgacgc cgtgttcagg gtcgccctca aggtcggggc
     2821 cgccctgtac ccggacgacg gagccgacgc cgaaatactg ttcaggaacg ccgaggccgc
     2881 gctgaaaaag gccaaggcgg gcggcgatcg ctacctgttc cacacgcaga aaatgaccga
     2941 aacggtggct ggcacgctca ccctcgaaaa ccagctgcgc caggcgctcg ataacgatga
     3001 attcgtgctc cattaccagc ccaaggtaaa cctcgtgagc ggcaggatga ccggcgcgga
     3061 ggcgctgatc cggtggaacg atccgcgcac gggcctggtg ccgccggggc agttcattcc
     3121 gatcctggaa gaaaccggat tgatccatga agtcgggcgc tgggcgctgc gaaaggccgt
     3181 cgaagactac ctgcgctggc gcgccgcggg cctgtccgtc gtgcgcatcg cggtgaacgt
     3241 gtcaccgctg caactgcgca atcgcagctt cgtcgccgag gtcagggaag ccatcggaat
     3301 cgacgcgcag tcggcggccg ggctggagct ggaaatcacc gagagcctga tcatggagga
     3361 cgtcaagcac agcatcgtca ccctgcaggc catccgcgcc ctgggtgtca ccgtcgccat
     3421 cgacgacttc ggcaccggct tctcctcgtt gagctacctc gccaggctgc cggtggacac
     3481 gctgaaaatc gaccactcgt tcgtgacgga catgattgcc ggaccggagg gactggcgct
     3541 ggtgtccacc attatcaacc tggcgcactc gctcaggctc aaggtggtgg cggagggcgt
     3601 cgaaaccgag gagcaatcac gcctgctccg gttgctgcgc tgcgacgaaa tgcagggctt
     3661 tgtcttcagc aaaccggtgc cgggcgaggt cttcgaggcg aggttcctgg ctctgcccgc
     3721 cgctgcgtga cgataaatga cgaagatgct gatcgcaaat gagtcacccg ccaagcgcg
//
LOCUS       DBNGSG010000447         3235 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3541438, whole genome shotgun sequence.
ACCESSION   DBNGSG010000447 DBNGSG010000000
VERSION     DBNGSG010000447.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3541438"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..1491
                     /gene="tig"
                     /locus_tag="AC158Q_11615"
     CDS             184..1491
                     /gene="tig"
                     /locus_tag="AC158Q_11615"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIR3340465.1"
                     /translation="MQANLETLSALERRLSVALPSADIDSEVESRLKNLSRTVKMHGF
                     RPGKVPFKVVVQYYGPQVRQEVLGDAMQKSFGEAVRQQNLKVVGYPRFELKPPAEGAG
                     ELQYSATFEIYPDVVVGDISRAAIERPLLEVSEAEVDKTVEIMRRQRAHYEPAERAAA
                     DGDRVTIDFRGTIDSAEFQGSSASGQQVLLGAGRLLPDFESNVVGMKAGESKTFDVRF
                     PDDYHGREVAGKTARFEVTAQEVAGPVLPEVSSDFAKSLGVVDGDIVKMRAEIRANLE
                     REVKLKLKIRVRDQVIQALLDATRIEAPRALVQMEARRMTEGARQELAARGVAVTEKT
                     PMPADLFEKQAERRVSTGLILGELMKAHGLHARPEQVRALVEEQAQSYERPEEVVKWF
                     YADPERLREIESAAAEENIVAWALGVAKVTDKPIEFDELMGKQ"
     gene            1491..2114
                     /gene="clpP"
                     /locus_tag="AC158Q_11620"
     CDS             1491..2114
                     /gene="clpP"
                     /locus_tag="AC158Q_11620"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007585897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HIR3340466.1"
                     /translation="MAHDTEPRGLGLIPMVIEQSGRGERAYDIYSRLLKERVVFLVGP
                     VTEVTANLIVAQMLFLESENPDKEISFYINSPGGSVSAGLAIYDTMQFIKPNVSTLCV
                     GQAASMGALLLAAGTKGKRSCLPNSRVMIHQPMGGFQGQASDIEIHAREILFLKGKLN
                     EIMARHTGRDMKDVERDTDRDNFLSAEQALAYGIVDKVLSNRTESVK"
     gene            2138..>3235
                     /gene="clpX"
                     /locus_tag="AC158Q_11625"
     CDS             2138..>3235
                     /gene="clpX"
                     /locus_tag="AC158Q_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004521258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HIR3340467.1"
                     /translation="MSDKVGGEKLLYCSFCGKSQHEVRKLIAGPSVFICDECIELCND
                     IIREETQGDHGSKGAKSDLPVPKEIRAILDQYVIGQDLAKKILSVAVYNHYNRLKSIT
                     KNDDVELAKSNILLIGPTGSGKTLLAQTLARLLNVPFVMADATTLTEAGYVGEDVENI
                     IQKLLQKCDYDVEKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGTI
                     ASVPPQGGRKHPNQEFVQVDTTNILFVCGGAFDGLDRIIRARSEKSGIGFGAEVSSRD
                     NRKDNTRGLRDVQPEDLIKYGLIPEFVGRLPVVATLEELDEAALIQILTEPKNALVRQ
                     YQKLFAMEGVELEFRDQALRAISRKALVRKTG"
BASE COUNT          658 a          960 c         1099 g          518 t
ORIGIN      
        1 gccgcaaggc atgggggttc gagtccctcc tttcgcacca cggggactct tctggcacgt
       61 ggtgcgagcc gggcgccggc ctccgatccg ttctttccga caagtcgccc gcggggccgg
      121 agcgcaagtg ttgtggccgc tatttgttat tgactaccgg gatcgttcta cgaccagcta
      181 aagatgcaag ccaacttgga aactttaagc gcactcgagc ggagattgag cgttgccctg
      241 ccgtccgccg acatcgacag cgaggtggag agccgcctga agaacctgtc gcgcacggtg
      301 aagatgcatg gctttcgccc cggcaaggtg ccgttcaagg tggtggtgca atactacggc
      361 ccgcaggtcc ggcaggaagt gctcggcgac gcgatgcaga agagcttcgg cgaggcggtg
      421 cgccagcaga acctgaaagt ggtgggttac ccgcggttcg agctgaagcc gccggccgag
      481 ggcgctggcg agctgcagta cagcgccacg ttcgaaatct accccgatgt ggtggtgggg
      541 gacatctccc gcgccgccat cgagcgcccg ttgctggagg tgagcgaggc agaggtggac
      601 aagaccgtcg agatcatgcg caggcagcgc gcgcactatg aaccggcgga gcgcgccgcg
      661 gcggacggcg accgcgtcac cattgatttt cgcggcacga tcgacagcgc cgaattccag
      721 ggatcgagcg cgagcggcca gcaggtgctg ctcggcgcgg gccgcctgtt gccggatttc
      781 gagtccaacg tggtcgggat gaaggcgggg gaatcgaaga cattcgacgt gcgtttcccg
      841 gacgattatc acggccggga agtggctgga aaaaccgcgc gcttcgaagt gaccgcccaa
      901 gaggtagccg gcccggtcct gcccgaagtc agctccgatt tcgcaaagag cctcggcgtc
      961 gtggatggcg atatcgtcaa gatgcgggcc gagatcaggg ccaacctcga gcgcgaggtc
     1021 aagctcaagt tgaagatccg cgtccgggac caggtcatac aggcgctgct cgacgccacc
     1081 cggatcgagg cccctagggc gctggtccag atggaggcac ggcgcatgac ggagggggcg
     1141 cgacaggagc tcgccgcgcg cggcgtcgcg gttacggaga agaccccgat gcccgccgac
     1201 ctgttcgaga aacaggcaga gcgccgggtc agcaccggtc tcatactcgg ggaactgatg
     1261 aaggcacacg gcctccatgc caggcccgag caggtgcggg cgctggtcga ggagcaggcg
     1321 cagagctacg agcggcccga ggaagtggtg aagtggttct acgcggatcc ggaacggctg
     1381 cgcgagatcg aatccgccgc ggcggaggaa aacattgtgg cctgggccct tggcgtcgcc
     1441 aaagtgaccg acaagccgat cgagttcgac gaattaatgg ggaagcaata atggcgcatg
     1501 acaccgaacc gaggggtctc ggattgatcc cgatggtgat cgagcagagc gggcggggcg
     1561 agcgcgccta cgacatctat tcgcgcctgc tgaaggagcg cgtggtattc ctggtaggtc
     1621 cggtgacgga ggtgaccgcc aacctgatcg ttgcgcagat gctgttcctc gagtccgaga
     1681 acccggacaa ggaaatttcg ttctacatca actctcccgg cggttcggtg tcggccgggc
     1741 tcgcgatcta cgacaccatg cagttcatca agcccaatgt ctccaccttg tgcgtcgggc
     1801 aggccgcaag catgggcgcc ctgctgctgg cggcgggcac gaaaggcaag cgttcctgcc
     1861 tgccgaactc gcgggtcatg atccaccagc cgatgggcgg tttccagggc caggcttccg
     1921 atatcgagat ccatgcgcgc gagatcctgt tcctgaaggg aaagctgaac gagatcatgg
     1981 ccaggcacac cggccgggac atgaaggacg tcgagcgcga taccgaccgc gacaatttcc
     2041 tgagcgccga acaggcgctc gcctacggga tcgtggacaa ggtgctgagc aacagaaccg
     2101 aaagcgtaaa atagggtcgt agacatgggg gcggactatg tctgacaagg ttggcgggga
     2161 aaagctgctc tactgctcgt tctgcggcaa gagccagcac gaggtgcgca agctcattgc
     2221 gggcccctcc gtgttcatct gcgatgagtg catcgagctc tgcaacgaca tcatccgcga
     2281 ggagacccag ggcgatcatg gctcgaaggg tgccaagtcg gacctgcccg tgcccaagga
     2341 gatccgggcc atcctcgacc agtacgtgat cgggcaggac ctcgccaaga agatcctctc
     2401 ggtggcggtc tacaatcatt acaatcggct gaagtcgatc accaagaatg acgacgtcga
     2461 gctcgccaag tcgaatatcc ttctgatcgg gccgacgggc tcgggcaaga cgctgcttgc
     2521 ccagacgctg gcgcgcctgt tgaacgttcc cttcgtgatg gcggacgcga ccacgctgac
     2581 cgaggcgggc tacgtcggcg aggacgtcga gaacatcatc cagaagctgc tgcagaaatg
     2641 cgactatgac gtcgagaagg cgcagcgcgg catcgtctac atcgacgaga tcgacaagat
     2701 atcgaggaag tccgacaacc cctcgatcac gcgggacgtg tcgggcgagg gcgtgcagca
     2761 ggcgctgttg aagctgatcg agggcaccat cgcctcggtg ccgccgcaag gcgggcgcaa
     2821 gcacccgaac caggaattcg tgcaggtgga caccaccaac atcctgttcg tctgcggcgg
     2881 cgcgttcgac gggctcgacc gcatcatccg cgcccgctcc gaaaaaagcg gcatcggctt
     2941 cggcgccgag gtgagctcgc gcgacaaccg caaggacaat acccggggac tgcgcgacgt
     3001 gcagcccgag gacctcatca agtacgggct gatccccgag ttcgtgggcc ggttgccggt
     3061 ggtggcgacc ctcgaggaac tcgacgaggc ggctctgatc caaatcctca cggaaccgaa
     3121 gaacgccctg gtacggcagt accagaagct gttcgccatg gagggcgtgg agctggagtt
     3181 ccgggaccag gctctgcggg cgatttcccg caaggccctg gttcgcaaga ccggc
//
LOCUS       DBNGSG010000448        15657 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_146281, whole genome shotgun sequence.
ACCESSION   DBNGSG010000448 DBNGSG010000000
VERSION     DBNGSG010000448.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 15657)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15657)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15657
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_146281"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2469
                     /locus_tag="AC158Q_11630"
     CDS             <1..2469
                     /locus_tag="AC158Q_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3340468.1"
                     /translation="LENSGRAALAVIAISLGVALGVAVHLVNASAINEFELAARHLAG
                     AADLVVRGPRAGFDEALYARIARLPGVEAANPAVELEAPLARRNDSLKIVGFDPLRAA
                     QVQPSLLPERSGMVLDLFDPGTVLLSPAAAEWLGLKSGDRLEIQVGTRAVSFRIAGLL
                     PQAAYRQRLGVMDIAAAQWRLERIGRLNRIDLRLKPGTDVEAFRRELQSTLPLGVHAA
                     SPETESGRGANLTRAYRLNLDMLALVALFTGAFLIFSSQVLALLRRRTQFALLRAMGV
                     TRGALAAQLLAEGAAIGVFGAALGILAGYALAHYALSLVGADLGAGYFRGIAASLDAE
                     PLTLALFFLLGVMFAVLGAAVPAWEAAHRPPALALHAGDEEEGLKSLKTLRWGIAAIA
                     AGLLLALAPSVNGLPVAGYCAIALVLLGAVLLMPQLAGACLGRLPLPARAAPAIAVAQ
                     LKAAPRQAAISIAAIVISFSLMASMLIMVSSFRVSLESWLERMLPADLYLRPARFGET
                     AFLTPAEQARIASTPGVRRAEFIRVQTLLLTPERPPVTLLAGPVEAGAPGKILPLTSP
                     PYAPRPDDPPPIWVSEVAADYYRLGVGDRVRLPIGDAPGLYTVAGVWRDYARQNGAIA
                     INRELYIEVTGDRLVNDAALWLDEGISLGEVQRTLRDRLREVPNMEIATTRELRAISL
                     GTFDRTFAITYALEIAAVVIGLFGVSVAFSAQALARRREFGVLRHIGMARREIGIMLA
                     CEGGLVAALGTAFGLATGWVVGMILIHVVNRQSFHWSMELHMPWLPLAGLATLVIAAA
                     VVTAVWSGRAAMSEDVVRAVREDW"
     gene            2548..3639
                     /locus_tag="AC158Q_11635"
     CDS             2548..3639
                     /locus_tag="AC158Q_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HIR3340469.1"
                     /translation="MSRRQVLLLLGGALCAPVNLGRATSNEQRATSFAPVVPGYKIQF
                     PRDEGSHPDFRIEWWYVTGWLTEPARPLGFQITFFRVRPDLKHDNPSRFAPRQILIAH
                     AALSDPSHGRLIHEQRAARAGFELAGSREGWTRVWIDEWQLLQAGSSYQARIAARDFS
                     LDLTFAATQPPLLQGDNGFSRKGPARESASYYYSIPHLKVGGTVTRGGNARRVTGTAW
                     MDHEWSSQYLEQEAVGWDWVGINLDDGSALMAFRMRGRQGGHFWAGGTLRHADGRLTV
                     FTPDDVRFVPRRNWKSARTGTTYPVSLLVTAGDREFSIEPIFDDQEHDTRATSGTIYW
                     EGAVRAFQSGRPAGLGYLELTGYWRRVNL"
     gene            complement(3750..4301)
                     /locus_tag="AC158Q_11640"
     CDS             complement(3750..4301)
                     /locus_tag="AC158Q_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340470.1"
                     /translation="MGIHQIQIRHDETEDRLLLRFSTTDNSEFRFWLTRRFTKRLWGM
                     LVQMLEWDQAVKQQVVPETRRTVLEIQHEGYSRQANYAKQFEEPTRETPRRLPLGEAP
                     VLLAKANGKKGDHGFQLLGLHPMKGQGIDITLDTRLLHIFTRLLREQVAKTDWDVNLA
                     LYETAQQSTPAENAQPLQPRKLN"
     gene            complement(4386..6989)
                     /gene="clpB"
                     /locus_tag="AC158Q_11645"
     CDS             complement(4386..6989)
                     /gene="clpB"
                     /locus_tag="AC158Q_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HIR3340471.1"
                     /translation="MRFDKFTTQFQQAFADAQSLAVGNDNPYIEPQHLLAALVNQPDG
                     STSSLLARAGVNVGALRAALKNAIDRLPKVEGTGGEVNVSRELGNLLNLTDKEAQKRG
                     DQFIASELFLLAAAQDKGETGKLIKQHGGSRNAIEKAVQAVRGTEGVQNPEAEGQREA
                     LKKYTLDLTERARSGKLDPVIGRDDEIRRVIQILQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLKSKRVLSLDMAALLAGAKYRGEFEERLKSVLKEIAQDAGQTIVF
                     IDELHTMVGAGKAEGAIDAGNMLKPALARGELHCVGATTLDEYRKYIEKDAALERRFQ
                     KVLVDEPSVESTIAILRGLQEKYEVHHGVDITDPAIVAAAELSHRYITDRFLPDKAID
                     LIDEAASRIKMEIDSKPEEMDKLDRRLIQLKIEREAVRKEKDEASKKRMAALEAEIKT
                     LEKDYSDLEEIWKAEKSEVQGSAHIKEEIDRVRAEIAQLQRQGKFDKVAELQYGKLPQ
                     LEAQLKHADRPRPGTGKPRLLRTQVGTEEIAEVVSRWTGIPVSKMMQGEREKLLQMEE
                     KLHQRVVGQDEAVRLVSDAIRRSRAGLGDPKRPYGSFLFLGPTGVGKTELCKALAEFL
                     FDSEDHLVRIDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTELVRRKPYSVILLDEIE
                     KAHHDVFNVLLQVLDDGRMTDGQGRTVDFKNTVIVMTSNLGSQTIQQMAGDDYQLIKT
                     AVLGEVKAHLRPEFVNRIDEMIVFHGLDEKHIKSIAKIQLALLEKRLAQLEMRIEVSD
                     AALKELAAAGFDPVYGARPLKRAIQSGIENPLARAILEGKFAAKDSIRVDHRKGGFTF
                     EKSQAVATAAA"
     gene            7120..8484
                     /locus_tag="AC158Q_11650"
     CDS             7120..8484
                     /locus_tag="AC158Q_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HIR3340472.1"
                     /translation="MGANAMDANEALLLARTQFGLNIAFHILFPSITIALAWFLVYFR
                     VQYARTQRREWLETYKLWTKIFALTFAMGVVSGITMSFQFGTNWPGFMNEVGNIAGPL
                     LAYEVLTAFFLEATFLGVMLFGMNRVPNWVHITSAVLVAAGTSLSAFWILALNSWMQT
                     PAGHVIADGQIIAGDWLQVIFNPSFPYRLVHMMLASGLTATFLIAGLSAWRLLKAPRD
                     AGAARTLRTGILVGCFLAPLQAWVGDLHGLNTLEHQPAKIAAVEAIWKTEKGVPLVLF
                     AIPNERTKRNDYAIEIPKGASLILRHDANAELQGLDAFAPDIPPVATVFFSFRVMVGI
                     GVCMIALAWFGLWATRRGRAAPKWLLWTFASFTFSGWVAVIAGWLVTEIGRQPWLVTG
                     ILRTAEAAGPASGAQLGASLTGYVLTYTLMLIAYMVVLTHLAGKGSAHDDPRAALMTR
                     TSPT"
     gene            8481..9473
                     /locus_tag="AC158Q_11655"
                     /pseudo
     CDS             8481..9473
                     /locus_tag="AC158Q_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559677.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006119 - oxidative phosphorylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
     gene            9806..11404
                     /locus_tag="AC158Q_11660"
     CDS             9806..11404
                     /locus_tag="AC158Q_11660"
                     /inference="COORDINATES: protein motif:HMM:NF015558.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HIR3340473.1"
                     /translation="MVYTQRTFWPLVIGIIMVGYAYLVQSGHLAPLIKFLNTGRYVGE
                     MLTLGYAFGGVAIAAGLWHLVAPRREGQADYYFSTVAGVMFIMLVAFVVKWGLDPLMA
                     NWGRAAQPTLGFNFASVMNLNYVVMGILAGIIVVNVFRVPQWAENGVRLSRLGLKTGV
                     ILLGALYSFAELAQLGKLSVVLIGVFVLGSVWLVLVIGTRRNLPNSMTGVLSSGVGVC
                     GVSAAVAAAPVVQAKSLEIAYTIGTILLFGVLCMFLFPIVGKQLGMGYVQFGAWAGTG
                     ILNSAQVAGAALAFQPGGIETLKVAEIFNITRVLFLPIIVLWLAVWYVRREQAAQRDV
                     QRVDAMQVVISKFPIFVLGFILMFLLSSTGIFAPARHYQGSYFDNSEKALMKKDRAGK
                     PIDNHLKAEEVATLQKELDKVKRDDQKAALARLIQNRKVMSEEDDVMVRGVINSKALS
                     KEGTAVLSKAHRVVRHTAAKIAKFRDLIAWFFTFGLVGLGMQITMASIRQAGGQPLVI
                     GSIVGVIKAVGSLIVVMLMVSETI"
     gene            11611..12075
                     /locus_tag="AC158Q_11665"
     CDS             11611..12075
                     /locus_tag="AC158Q_11665"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3340474.1"
                     /translation="MLKPDRVLIASHGTPGARAAERAALELASPAATIYHLVVVPDLW
                     KGMMGDDWLNNASTRSTYGRHVESQLAGEIEEHRLSLEREVRNAGRHYETRVVLGKPD
                     EALLEYAAEIRPDLVVIGAPRPPGMPGLRSRMRVEKLVGALGVALVVIPQSR"
     gene            12072..12665
                     /locus_tag="AC158Q_11670"
     CDS             12072..12665
                     /locus_tag="AC158Q_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340475.1"
                     /translation="MSGPGPAPADAAWVVIEVPLAPGDLAAFCSDLERLYRINPYLEF
                     RYWRGTAPSALAVAFRNLSNLREFELQLTLERASERDFTVHYDRGIKRSTRFEIEPAA
                     GASCLTITDAYHGADAADTGEVDRSLHAWGVALKEYLLRERRWRWCGPWRWYMRRVWV
                     PMKPSARRITFMILVVTLAEIALIALVLAIYWIEHRA"
     gene            complement(12685..14202)
                     /locus_tag="AC158Q_11675"
     CDS             complement(12685..14202)
                     /locus_tag="AC158Q_11675"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013784.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0097054 - L-glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HIR3340476.1"
                     /translation="MEGMLSVVSYGIGTILGFVLLGALFILIVHDVTQKKHTVLRNYP
                     VVGHLRFFFEDLGEYFRQYFFSGDRSEMPFNRATRGFVYRLAKGEGSILGFGSTNDLR
                     EPGSIIFVNHPFPVLEEDRLQTPSLVIGEGYCKHPFEARSIVNISGMSFGAISAPAVR
                     SLSRGAREAGCYMDTGEGGLSPHHIEGGCDIIMQMGTAKYGMRNHDGSFSPEKVRELA
                     NHVKAFEIKLSQGAKPGKGGVLPGGKVTEEIAGIRGIPAGKDSISPNRHYDIANMNEL
                     LDKVAYLRELTGRPVGIKTAIGGWDFVNELCDTVLRRGREYAPDFLAIDGGEGGSGAA
                     PQTLMDHMSLPIAEALPRVVDSLLQADLKGRIRVIAAGKLVTSARAAWALCVGADFIN
                     TARGFMFSLGCIQALRCHQNSCPTGVTTHNKRLQRGRVVEEKYLRVANYARGMNKEID
                     MIAHSCGCRHGRELKREHVRIVQTANQSIALNMLYPYPVVGSRATSKQATASAAA"
     gene            14431..15264
                     /gene="nadC"
                     /locus_tag="AC158Q_11680"
     CDS             14431..15264
                     /gene="nadC"
                     /locus_tag="AC158Q_11680"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HIR3340477.1"
                     /translation="MDLASEIERNVAAALAEDVGDGDATAPLTPAGKPARAVVTCRQE
                     AIVCVQPWFDACFLKLDPKARIQWAVHEGARVIAGTGVCTVEGEARALLAAERSGINF
                     LQTLSAVATATRRLVDAAAGTRARIVDTRKTLPGLRLAQKYAVRIGGGANHRMGLYDA
                     MLIKENHIAAAGGIRQVLAQAKKAAKPGVWVQIEVENLDQLREALDAGATMILLDNMS
                     IETMAEAVRISNDRAELEASGGITLANVRAIAETGVHRISSGALTKDVKAVDFSMRFE
                     T"
     gene            15455..15657
                     /locus_tag="AC158Q_11685"
                     /pseudo
     CDS             15455..15657
                     /locus_tag="AC158Q_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612027.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         2536 a         5112 c         4958 g         3051 t
ORIGIN      
        1 ctggaaaact ccggccgtgc tgcgctcgcc gtgatcgcca tttcgctggg agtggcgctc
       61 ggggttgccg ttcacctcgt caatgccagc gcgatcaacg aattcgagct cgccgcccgg
      121 caccttgcag gcgcagccga tctggtggtg cgcggcccgc gcgccggatt tgacgaggcg
      181 ctctatgccc ggatcgcgcg cctgcccggg gtcgaggcgg ccaacccggc ggtggaactc
      241 gaagcgcccc tcgcccggcg caacgacagc ctgaagatcg tcggatttga cccgttgcgc
      301 gccgcgcagg tgcagccctc tctgctgccg gaacgctcgg gcatggttct cgacctcttc
      361 gatcccggta cggtgctgct cagcccggcc gccgcggaat ggctgggatt gaagtcgggc
      421 gaccgtctgg agatacaggt aggcacgcgc gcggtttcct tcaggatcgc gggcctgctg
      481 ccacaggccg cctatcgcca gcgcctgggt gtcatggaca tcgcggccgc gcaatggcgg
      541 ctcgagcgca ttggccgcct gaatcgaatc gacctgcggc tgaaaccggg caccgatgtc
      601 gaggctttcc gccgcgaatt gcagtccacc ctcccccttg gcgtgcacgc cgcgtccccc
      661 gaaaccgaaa gcgggcgcgg ggccaacctg acgcgcgcct accgcctgaa cctcgacatg
      721 ctggcgctgg tcgccctgtt caccggtgcg ttcctgatct tttcatcaca ggtactggcg
      781 ctgctgcgcc ggcgcacgca gttcgcattg ttgcgcgcga tgggcgttac gcgcggcgcg
      841 ctggccgcgc aactcctggc cgagggcgcg gcaatcggcg tcttcggcgc ggcgctgggc
      901 attctggcgg gatatgcgct ggcgcattat gcgctctcgc tggtcggcgc cgatctgggt
      961 gcgggctatt tccgcggcat tgccgcgagc ctcgatgccg aaccgctgac gctggcgctg
     1021 tttttcctcc tcggcgtgat gttcgcggtg ctgggcgccg ccgtgcccgc ttgggaagcc
     1081 gcgcaccggc caccggccct ggcattgcac gcgggcgacg aggaagaagg tctgaaaagc
     1141 ctgaaaaccc tgcggtgggg cattgcagcc atcgcggcag gtctgctgct ggcgctcgcg
     1201 ccgtccgtca acgggcttcc ggtcgccggt tattgtgcga tcgcactggt gctgctggga
     1261 gcggtgctgc tcatgccgca gctcgccggt gcgtgcctcg gccggctgcc cctgcccgcg
     1321 cgtgccgcac ctgccatcgc cgtcgcgcag ctcaaggctg ctccgcgcca ggcggcgatc
     1381 agcatcgcgg cgatcgtcat cagcttcagc ctcatggcct cgatgctgat catggtgagc
     1441 tcgttccggg tgtcgctcga atcctggctc gagcgcatgc tgcccgcgga cctctacctg
     1501 cggcccgcgc gctttggcga aacggcattc ctcacccctg cggagcaggc gcgcatcgct
     1561 tccacacccg gtgtcaggcg ggcggaattc atccgcgtgc aaaccctgct gctcacgccc
     1621 gagcgcccgc cggtcacgct gctggcggga ccggtcgagg ccggcgcgcc cgggaaaatc
     1681 ctgccgttga ccagcccgcc ttacgcgccg cggccggatg atccccctcc gatatgggta
     1741 tccgaggtgg ctgcggatta ctaccgttta ggggtcggag accgggttcg cctgcccatc
     1801 ggtgacgcgc ccgggcttta taccgtggcg ggggtgtggc gcgactacgc gcgccagaat
     1861 ggcgcaatcg caatcaaccg cgagctgtat atcgaggtga caggcgaccg cctcgtgaac
     1921 gatgctgcgc tgtggctcga cgaagggata tctctcggcg aagtccagcg caccctgcgc
     1981 gaccggctcc gcgaggttcc caacatggaa atcgccacca cgcgcgagct gcgcgccatc
     2041 tccctcggaa ctttcgaccg cacttttgcc atcacctatg cgctggaaat cgcggcggta
     2101 gtgatcggcc tgttcggcgt cagcgtggcg ttcagcgccc aggcgcttgc ccgccgccgc
     2161 gagttcggcg tgctgcgcca catcggcatg gcgcgccgtg aaatcggcat catgctggcc
     2221 tgcgagggcg gactggtcgc cgcgctcggg accgccttcg gcctggcgac gggctgggtg
     2281 gtgggaatga tcctgatcca cgtcgtcaac cgtcaatcct tccattggag catggaactc
     2341 catatgccgt ggctgccgct cgcggggctg gcgacgctgg tcatcgccgc tgcggtcgtg
     2401 acggccgtgt ggagcggccg cgcggcgatg agcgaggatg tcgtacgagc ggtgcgcgag
     2461 gactggtgat tcgtttctcg ttactcgttt ctcgtttctc gttactcgtt ttagtagctt
     2521 atggatgaag aaccgaaaat cagtagaatg tccaggcggc aggtgttgct gttgctgggc
     2581 ggcgcgcttt gcgcgccggt aaacctggga cgagcaacga gcaacgagca acgagccact
     2641 tctttcgccc ccgtcgtccc gggctacaag attcagttcc cccgggacga aggcagccac
     2701 ccggacttcc ggatcgaatg gtggtacgta acgggctggc tcactgaacc ggcccggccg
     2761 ctcggcttcc agatcacctt cttccgggtc aggccggact tgaagcacga caacccgagc
     2821 cgctttgcgc cgcgccagat tctcattgcc cacgccgcgc tctcagatcc ttcgcacggc
     2881 cggttgatcc acgagcagcg cgcggcgcga gcgggctttg aactggccgg ttcccgggaa
     2941 ggatggaccc gggtctggat agacgagtgg cagctgctgc aagccggttc ttcctatcag
     3001 gcgcggattg cggcgcggga cttttcgctc gatctgacgt tcgctgcaac gcaaccgcca
     3061 ttgttgcagg gagacaacgg gttcagccgc aagggacccg cacgggaatc agccagctat
     3121 tactacagca tccctcatct gaaggtcggc ggtacggtta cccgcggcgg gaacgcgcga
     3181 cgcgtcacgg gaaccgcgtg gatggaccat gagtggtcct cccaatacct ggagcaggaa
     3241 gccgtgggtt gggactgggt tggcatcaat ctcgacgacg gatccgcgct gatggcgttt
     3301 cgcatgcgcg gccggcaagg cggccatttc tgggcgggcg gcacgttacg ccatgccgac
     3361 ggccggctca cggtgttcac gccggacgac gtgcgcttcg taccccgccg caactggaaa
     3421 tcggcgcgca ccgggacaac ttacccggta tcgctcctgg tcacggcagg cgatcgtgaa
     3481 ttttcaatcg aaccgatctt tgacgaccag gaacacgata cccgcgcgac gagcgggacg
     3541 atttactggg aaggcgccgt gcgcgcgttt cagagcggca ggccagcggg cctcggctat
     3601 ctcgagctga ccggctactg gcgccgcgtc aacctgtaaa cgtatcctgg acccggattg
     3661 cgacaagcga caatttgcgc gcgctgagcg gcgtctagtc cgtctccaac gcgcgcggta
     3721 aggcgggcct gaaaggcccc gatttgacgc tagttcagct tgcgcggctg cagtggctgc
     3781 gcattctccg ccggcgtgga ttgctgcgcc gtctcgtaga gcgcgagatt cacatcccag
     3841 tcggtcttcg ccacctgctc gcgcagcagc cgcgtgaata tatgcagaag cctggtgtcg
     3901 agggtgatgt ctattccctg ccccttcatc ggatgtaggc ccagcagctg gaagccgtga
     3961 tcgccctttt tgccgttcgc cttggccagc agcaccggtg cctcgcccag cggcaggcgg
     4021 cgcggggttt cccgggttgg ctcctcgaac tgcttcgcgt aattcgcctg tctcgagtag
     4081 ccctcgtgct gtatttcaag cacggtgcgg cgcgtctccg gcaccacctg ctgcttcact
     4141 gcctggtccc attccagcat ctgcaccagc attccccaca gccgcttggt gaatcggcgc
     4201 gtaagccaga accggaattc agagttatcc gtggtcgaga accgcaacag gaggcggtcc
     4261 tcggtttcgt cgtggcgaat ctggatctgg tgtatgccca tcaggcgctg gccgtcagct
     4321 ttcagccact agcttaaacc gttttgcggc cggttcaagc ttccgactgg ccgcttgcgc
     4381 cgagctcagg ccgccgcggt ggcaacggcc tgcgatttct caaaagtgaa gccacccttc
     4441 ctgtggtcca ctcggattga gtccttggcg gcgaactttc cctcgagaat cgccctggcc
     4501 agcggatttt cgatgcccga ctgaatcgcg cgcttcaatg gccgcgcacc gtagaccggg
     4561 tcaaagccgg ccgccgcgag ttctttcaat gccgcgtcgc tcacctcgat ccgcatttcg
     4621 agctgcgcca gccgcttctc gagcagcgcg agttggatct tcgcaatgga cttgatgtgc
     4681 ttctcgtcaa gcccgtggaa aacgatcatc tcgtcgatgc ggttgacgaa ttccggccgc
     4741 aggtgcgcct tgacctcgcc cagcaccgcg gtcttgatca actggtagtc gtcgcccgcc
     4801 atctgctgga tggtctgcga gcccaggttc gaggtcatca cgatcacggt gttcttgaaa
     4861 tccacggtgc ggccctggcc atcggtcatg cgcccgtcgt cgagcacctg cagcagcacg
     4921 ttgaagacgt catggtgcgc cttttcgatc tcgtcgagca ggatcaccga gtacggcttg
     4981 cggcgaacca gctcggtaag atagccgcct tcctcgtagc ccacgtagcc gggaggcgcg
     5041 ccgatcaacc gcgcgaccga gtgcttttcc atgaactccg acatgtcgat gcggacgagg
     5101 tgatcctcgg aatcgaacag gaattccgcg agcgccttgc acagctcggt cttgcccacg
     5161 ccggtcggcc cgaggaacag gaacgagccg tacgggcgct tggggtcgcc caggcccgcg
     5221 cgcgagcggc ggatggcgtc cgatacgagc ctgaccgcct cgtcctggcc gaccacgcgc
     5281 tggtgcagct tctcttccat ctgcaggagt ttttcgcgct ccccctgcat catcttggac
     5341 acgggaatcc cggtccagcg cgagacgact tccgcaattt cttccgttcc gacctgggtg
     5401 cgcagcagcc gcggcttgcc cgtgcccggc ctgggccggt ccgcgtgctt caactgcgct
     5461 tcgagctgcg gcagcttgcc gtactggagc tcagcgacct tgtcgaactt gccctgccgc
     5521 tgcaactggg cgatctcggc gcgcacgcgg tcgatttctt ccttgatgtg cgccgacccc
     5581 tgcacctcgg acttctccgc cttccagatc tcctcgaggt cggagtagtc cttttcgagc
     5641 gtcttgatct cggcctcgag ggccgccatc cgcttttttg aggcctcgtc cttttccttc
     5701 cgcaccgcct cgcgctcgat tttcagctgg atcagccgcc ggtcgagctt gtccatctcc
     5761 tcgggcttgg agtcgatctc catcttgatg cgggaggccg cctcgtcaat taagtcgatc
     5821 gccttgtccg gcaggaagcg gtcggtgatg taacgatgcg agagctcggc cgccgcgacg
     5881 attgcggggt cggtgatgtc cacgccgtga tggacctcgt acttttcctg caggccgcgc
     5941 aggatggcga tggtggattc caccgacggc tcgtccacca gcactttctg gaagcgccgc
     6001 tcgagcgcgg cgtccttctc gatgtacttg cggtactcgt cgagggtggt cgcgccgacg
     6061 cagtgcagct cgccgcgcgc gagcgcgggc ttcagcatgt tgccggcgtc gatggcgccc
     6121 tcggccttgc cggcgccgac catggtatgc agttcgtcaa tgaaaacgat ggtctggccg
     6181 gcgtcctgtg cgatttcctt caacacggac ttgaggcgct cttcgaattc tccgcggtac
     6241 ttggcgcccg cgagcaatgc cgccatgtcg agcgaaagaa cccgcttgct cttgagcgat
     6301 tccggtacct cgccgttgac gatgcgctgg gcgagccctt ccacgatcgc ggtcttgccc
     6361 acgccgggct cgccgatgag gaccggattg ttcttggtgc ggcgctgcag gatctggatc
     6421 acgcgccgga tctcgtcgtc acgcccgatc acggggtcga gcttgccgct gcgggcgcgc
     6481 tcggtgagat cgagcgtgta cttcttcaat gcttcgcgct gcccctcggc ctccgggttc
     6541 tggacgcctt cggtaccgcg cacggcctgg accgccttct cgatcgcatt gcggctgccg
     6601 ccgtgctgct tgatcagctt gccggtctcg cctttgtcct gggcggccgc gagcaggaac
     6661 agttcggatg cgatgaactg gtcgccgcgc ttctgagctt ccttgtcggt gaggttgaga
     6721 agattgccca gttcgcgcga gacattgacc tcgccgcccg tgccctccac cttgggcagg
     6781 cggtcgatgg cgttcttcaa cgccgcacgc aatgcgccga cgttgacgcc ggcgcgcgcg
     6841 agcagcgacg aggtgctgcc atcgggctgg ttgaccagcg cggccagcag gtgctgcggc
     6901 tcgatatacg ggttgtcgtt gcccaccgcc aggctctggg catccgcaaa ggcctgctgg
     6961 aactgggtcg tgaatttgtc gaatcgcatg gtttcgccgt aacacgttga ctacaaggca
     7021 tattgggaac agggccgccc ttttcaaggg gggtggccgc acgcccggaa aattccagct
     7081 tacgccagcc cctctactcc gcggctgccc ggccgtagaa tgggcgcaaa cgccatggat
     7141 gccaacgaag ccctcctgct cgcccgcacc cagttcgggc tgaatattgc gtttcacatc
     7201 ctgtttccga gcatcaccat cgcgctcgcc tggtttctcg tctacttccg cgtacagtac
     7261 gcgcgcacgc agcgccgcga gtggctggaa acctacaagt tgtggaccaa gatcttcgcg
     7321 ctcactttcg cgatgggcgt ggtctccggt atcacgatgt cgttccagtt cggcaccaat
     7381 tggcccgggt tcatgaacga ggtcggcaac atcgccggtc cgcttctcgc ctacgaagtg
     7441 ctcaccgcct tcttcctcga ggccactttt ctcggcgtga tgctgttcgg catgaaccgg
     7501 gtgccgaact gggtgcacat cacctcggcc gtgctggtcg ccgccggcac ctcgctctcg
     7561 gcgttctgga tcctcgcgct caattcctgg atgcagacgc ccgccggaca cgtcatcgcc
     7621 gacggccaga tcatcgcggg cgactggctg caggtgattt tcaatccctc cttcccgtac
     7681 cggctcgttc acatgatgct cgcctcgggg ctcaccgcga cgttcctcat cgcggggctc
     7741 tcggcctggc ggctgctcaa ggcgccgcgc gacgccggcg ccgcgcgcac cttgcgaacc
     7801 ggcatcctgg tcgggtgctt cctcgcgccg ctgcaggcgt gggtcggcga cctgcacggg
     7861 ttgaacacgc tcgagcacca gccggcaaag atcgccgccg tcgaagccat ctggaagacg
     7921 gagaagggcg tgccgcttgt cctcttcgcc attcccaacg aacgcacgaa gcgcaacgat
     7981 tacgcgatcg agatacccaa gggcgcgagc ctgatcctca ggcacgatgc caatgccgaa
     8041 ctgcagggtc tcgatgcctt cgcgcccgac attccgccgg tcgcgacggt gttcttctcc
     8101 ttccgcgtga tggtcggcat cggcgtctgc atgatcgcgc tcgcctggtt cggcttgtgg
     8161 gcgacgcgcc gcggccgcgc cgcgccgaag tggctgctgt ggacgttcgc cagcttcacg
     8221 ttctcgggct gggtcgcggt catcgccggc tggctggtga ccgagatcgg ccgtcagccg
     8281 tggctggtaa cgggaatcct gcgcaccgcc gaagccgcgg gaccggcatc cggtgcccag
     8341 ctcggcgcaa gcctgacggg ctacgtgctg acatacacgc tgatgctcat cgcgtacatg
     8401 gtcgtgctga cgcacctcgc cggcaaaggc agtgcgcatg acgacccgcg cgccgcgctc
     8461 atgacccgga cgtcgccgac atgaccttcg acctgcccgt catcttcgcc cttctcatgg
     8521 gcgtggcgat cctggcgtat gtcgtgctgg acggctacga cctcggcgtc ggcatgctgc
     8581 tgccggcggg cacaccgcgc gagcagaact tcatggtgtc ctcgatcggt ccgttctggg
     8641 atgccaatga gacctggctg gtattgggcg tggggctcct gcttgtcgcg tttccgaccg
     8701 cgcacggcgt cgtgctgggc gccctgtacc tgccggtggt ggtcatgctc atcggtctca
     8761 tgctgcgcgg cgtcgccttc gagtttcgcg tgaaggccga aggctggcac cgcgagctgt
     8821 ggaacgggtt gttctggttc ggctccttcc tcgcctcgct cgcgcagggg ctgatgctcg
     8881 ggtcctacat aacgggtttc gagccgggtt tcggctactt ccttttctcg ctggtcgtcg
     8941 cgggtgggct gtgcggcggc tacgtgctgc tgggcgccac ctggctcatc ctgcgcaccg
     9001 agggagaatt gcagaagaag gcggtcggct ggacgcgctg gggcctgctt tgggtcgccc
     9061 tcggcgtcgc gctggtcagt atcgccacgc cgctcgcgag tgaatccgtg cgctacaaat
     9121 ggttcgactt tccgcgcacg ctgggattga tgctgctgcc cgctgccagc gccgcggcgt
     9181 ggctctgggt ttggtcactg accggccgga tttagcgcgg gctgcccgcg ccggaatggg
     9241 cgccgttcgc cggcgccgtg gccatctatg tgctggcgtt cctggggctc gcgtacagcc
     9301 tgttccccta cgtcgtgatc gaccggatga cgatctggga cgcggccgcg cacccgtccg
     9361 cgctgaaagt gatgctgatc ggcgcgctga tcgtgctgcc gttcatcatc gcctacacgg
     9421 tgttttccta ccgcgttttc cgcggcaagg cctcggaaaa gctctacgac tgaccgcccg
     9481 ggttgccaac cggtctcggc gtatcagcca gcccgagatc gcccgttggc gatgaacgcc
     9541 gcggccgcac agcttcatta gaaatgcaaa agggcccatc gaaaatactt atgctgttct
     9601 gcagcggtcg catggacaat gcgaccgcgt ccttcgcagc gtgaatactt gacttcccat
     9661 gagggccgac taacactatg gatcgttgat tgttgcacct gcaatgcgcg tgcaactggc
     9721 gcacgaaccg ataaaagcaa ctccgagcca acccgcggcg ttcgcgggaa cgcctgactg
     9781 aaagttgcca acaagggagg agcgcatggt ctacacgcaa cgaacgttct ggccgctggt
     9841 catcggcatc atcatggtgg gctacgccta tctggtccag agcggccatt tggcacccct
     9901 catcaagttc ctcaataccg ggcgttacgt cggcgaaatg ctgacgctcg gctacgcttt
     9961 cggcggcgtc gcgatcgcgg cgggcctctg gcacctcgtc gcccctcgac gcgagggaca
    10021 ggccgattac tacttctcca ccgtcgcggg tgtcatgttc atcatgctgg tagcctttgt
    10081 cgtcaaatgg ggtctggatc ccctgatggc gaattggggg cgcgccgcac agcccacgct
    10141 cggtttcaat ttcgccagcg tgatgaacct caattacgtg gtgatgggca tcctcgccgg
    10201 catcatcgtg gtaaacgttt tccgcgttcc ccagtgggct gaaaacggcg ttcgcctctc
    10261 gcggctggga ctcaagaccg gcgtcattct gctgggcgcg ctgtacagct tcgccgagct
    10321 tgcccagctc gggaaacttt ccgtggtcct gatcggcgtc ttcgtcctcg gctccgtctg
    10381 gctggtcctg gtcatcggca cgcgccgcaa tctgccgaat tcgatgaccg gcgtgttgtc
    10441 ctccggagtc ggcgtgtgcg gcgtgtccgc cgcggtcgcg gccgccccgg tggtccaggc
    10501 gaaatcgctg gaaatcgcct acacgatcgg cacgatcctg ctgttcggcg tgttgtgcat
    10561 gttcctgttt ccgatcgtgg gcaagcagct tggcatgggc tacgtccagt tcggggcgtg
    10621 ggccggcacc ggcattctca attccgccca ggttgccggc gccgcgctcg cgttccagcc
    10681 gggcggaatc gaaacgctga aggtcgcgga aatattcaac atcacgcgag tcctgttcct
    10741 gcccatcatc gtcctgtggc tcgcagtgtg gtacgtccgg cgcgaacagg ctgcgcaacg
    10801 cgatgtccag cgggtcgacg cgatgcaggt cgtcatcagc aagttcccga tcttcgtgct
    10861 cggcttcatc ctgatgttcc tgctgtcctc caccggcatc ttcgcgccgg cgcgccacta
    10921 ccagggttcc tatttcgaca acagcgaaaa agcgctgatg aaaaaggacc gcgccggaaa
    10981 gccgatcgac aatcacctca aggccgagga ggtcgcgacg ctgcagaagg aactcgacaa
    11041 ggtcaaacgc gatgaccaga aggcggcgct ggcgcggctc atccagaaca ggaaggtcat
    11101 gtccgaggag gacgacgtga tggtgcgcgg cgtgatcaat tccaaggcac tctccaagga
    11161 gggcaccgcg gtcctgtcca aggcccaccg cgtggtccgg cacactgctg ccaagatcgc
    11221 caagttccgg gacctgatcg cgtggttctt cacattcggc ctggtggggc tcggcatgca
    11281 gatcaccatg gcctcgatcc ggcaggctgg cggccagccg ctcgtcatcg gctccatcgt
    11341 cggcgtgatc aaggcggtcg ggtcgctcat cgtcgtcatg ctcatggtct ccgagaccat
    11401 ctgagcgcaa ggagaacaac atggaagaca aattcgaccg cggttccgcg ctcacgattt
    11461 tccacagcga ccgtcatgaa gacctcatgg ctctcatact ggcgttcgcg atcgcagcgc
    11521 tcgtttactt cctggtctga ttcccgggcc ccgacccgac acgcaccccg gtgaacgcgc
    11581 cgcctggcgc ggcaccgggg ttttttttcc atgctgaagc cagaccgcgt cctgatcgcc
    11641 agccacggca cgcccggcgc gcgcgcagcc gagcgcgccg cgctcgagct ggcatcgccg
    11701 gcggccacga tctatcacct cgtcgtggtg cccgacctct ggaaaggcat gatgggcgat
    11761 gactggctca acaatgcttc cacccgctcg acctatggcc ggcacgtgga atcccagctc
    11821 gcgggggaga tcgaggagca ccggctgtcg ctcgagcgag aagtgcgcaa cgccggcagg
    11881 cattacgaaa ccagggtcgt gctcggcaag cccgacgaag cgctgctcga gtatgctgcc
    11941 gagattcgtc ccgatctcgt cgtcatcggc gcgccccgtc cgcccgggat gccgggactg
    12001 cgctcccgca tgcgggtcga aaaactcgtt ggagcgctcg gcgttgccct cgtcgtaatt
    12061 ccacaatcgc gatgagcggt cccggcccgg cacccgccga cgcggcgtgg gtggtgatcg
    12121 aggtgccgct agcccccggg gatctagccg cgttctgcag cgatctcgag aggctctacc
    12181 gcatcaatcc ctatctggag ttccggtatt ggcgcggcac cgcgcccagc gcgcttgcag
    12241 tggcattccg gaacctgagc aacctgcgcg agttcgaact gcagttgacg ctagaacggg
    12301 cctcggagcg ggatttcacc gtgcactacg accggggcat caagcgcagc acccgtttcg
    12361 agatcgagcc cgccgcaggc gccagctgcc ttacgatcac ggacgcgtac cacggtgccg
    12421 atgccgcgga cacgggggaa gtcgaccgca gcctgcacgc atggggcgtc gcgctgaagg
    12481 agtatttgct gcgggaacgg cgctggagat ggtgcggccc ctggcgctgg tacatgcgca
    12541 gggtgtgggt gccgatgaag ccgtccgccc gccgcatcac cttcatgatc ctggtggtga
    12601 cgctcgccga aatcgcgctg attgccctgg tgctggcgat ctactggatc gagcaccgcg
    12661 cgtgattccg tcgccggttc tttcctaggc ggccgcgctg gccgtggcct gcttcgatgt
    12721 cgcgcgcgag ccgaccacgg ggtacggata gagcatgttg agcgcaatgc tctggttggc
    12781 ggtctgcacg atgcgcacgt gctcgcgctt gagttcccgc ccatggcggc agccgcacga
    12841 gtgcgcaatc atgtcgattt ccttgttcat tccgcgcgcg tagttcgcga cccgcaggta
    12901 cttctcttcc accacccgcc cgcgctgcag tcgcttgttg tgggtcgtca ccccggtcgg
    12961 gcaggaattc tggtggcagc gcaacgcctg gatgcagccg agcgaaaaca tgaagccgcg
    13021 ggccgtgttg atgaagtccg cccccacgca cagcgcccac gccgcgcgcg ccgaagtgac
    13081 gagcttgccc gcggcgatca cgcggatgcg tcccttcagg tcggcctgca gcagggagtc
    13141 caccacgcgc ggcagggctt cggcgatcgg cagcgacatg tggtccatca gcgtctgcgg
    13201 ggcggcgccg ctgccgccct cgccgccgtc gatcgccagg aaatcaggcg cgtactctcg
    13261 gccccggcgc agtaccgtgt cgcacagctc attgacgaaa tcccagccgc cgatggcggt
    13321 cttgatgcca accggccgtc cggtgagctc gcgcaggtaa gccaccttgt ccagcagctc
    13381 gttcatgttg gcgatgtcgt agtggcggtt ggggctgatc gaatccttgc cggccggtat
    13441 cccgcgtatc ccggcgatct cctcggtcac cttgccgccc ggcagcaccc cgcctttgcc
    13501 cggtttcgcg ccctgcgaga gcttgatctc gaatgccttc acgtgattgg ccagctccct
    13561 caccttttcc ggggaaaagc tgccgtcgtg attgcgcatg ccgtatttcg cggtgcccat
    13621 ctgcatgatg atgtcgcagc cgccctcgat atgatgcgga gacagaccgc cctccccggt
    13681 atccatgtaa caacccgctt cccgcgctcc gcgcgagagc gagcgcaccg ccggcgccga
    13741 gatcgcgcca aagctcatgc cgctaatgtt gacgatcgag cgcgcctcga atggatgctt
    13801 gcagtatcct tccccgatca cgagcgaagg cgtctgcagg cggtcctcct cgagcaccgg
    13861 gaacgggtga ttgacgaaaa tgatggaacc tggctctcgc aggtcgttgg tcgagccgaa
    13921 gccgagtatg ctgccctcgc ccttggccag ccggtagaca aagccccgcg tggcgcggtt
    13981 gaacggcatc tcgctgcggt cgcccgagaa gaaatactgg cggaagtact cgccgagatc
    14041 ctcgaagaaa aagcgcaggt gcccgacgac cggatagttg cgcagcacgg tgtgtttctt
    14101 ctgggtgacg tcgtgaacga tcaggatgaa caacgcgccc agcagcacga atcccagtat
    14161 cgtgccgata ccgtaggaaa ccacgctcaa catgccttcc atcccgcccc ctccgatacg
    14221 ctagaatcga actacatcat caaccgccaa tgaacgccga tccactacaa aatttcacaa
    14281 gaccatctga actgcgtcga gcattgcgga agctgttata aaaagcctgg ttccgtgaat
    14341 cctgcaagtt cttttttgtt attgtactta tcggcgtgta tcggcgttca tcggcggtta
    14401 catcagccct gtaaactata gagcaaatcg gtggacttgg catccgagat cgagcgcaac
    14461 gtagccgccg ccctggccga ggacgtgggc gatggagacg ccaccgcgcc actcaccccc
    14521 gccggcaagc cggcccgggc cgtggtgacg tgccggcagg aagcgatcgt ctgcgtacag
    14581 ccctggttcg acgcctgctt cctgaagctc gaccccaaag cccgcatcca gtgggcggtc
    14641 catgaaggtg ccagggtcat cgccggcacg ggagtatgca cggtggaagg cgaagcacgc
    14701 gcgttgctcg ctgccgaacg cagcgggatc aacttcctgc agactctctc cgccgtcgcg
    14761 accgccacgc gccggctggt ggatgccgct gcgggtaccc gggcaaggat cgtggacacg
    14821 cgcaagaccc tccccggact gcgcctggca cagaaatacg cggtacgcat tggcggcggc
    14881 gccaaccacc gcatgggact ctacgacgcc atgttgatca aggaaaacca tatcgcggcg
    14941 gcaggcggca tccgccaggt gctggcgcag gcgaagaaag ccgcgaaacc gggcgtgtgg
    15001 gttcagatcg aagtcgagaa tctcgatcag ctccgcgaag ccctcgacgc cggtgcgacc
    15061 atgatcctgc tcgacaacat gagcatcgaa acgatggcgg aggcagtcag gatttccaat
    15121 gatcgcgcgg aactggaagc gtccggcggc atcacgctgg cgaacgtgcg cgcgatcgcg
    15181 gagacgggcg tgcaccgtat ctcgagcgga gcgctgacga aagacgtgaa ggcggtggat
    15241 ttttccatga ggttcgagac gtaaagctta gacacgaagg acacaaagga taccaaggaa
    15301 agatctaatc gaaccgccaa gacgccaagg ccgccaagaa catcaatgat tattcgagcc
    15361 tggccagggt cagtgtggac ttaccaggct ttaacgtaca ccttttgggt cgccgtaaga
    15421 aggaggacca atgggtggtc gaagcaagtc gcatggtgtc cgagccaagt atgccttcat
    15481 caaggcacat cgtagtgaat tcgacacggc gaggatgtgt cgcctcttag aggtgtcacg
    15541 cagcggttat tacgagtggc tgcggaaacc cttatctaac cgtgcgttag aggatcagcg
    15601 gttactcggc ctgatccgcg cgtcttacac ggcgagccat ggtgtctatg gcgcccc
//
LOCUS       DBNGSG010000449         3473 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4391141, whole genome shotgun sequence.
ACCESSION   DBNGSG010000449 DBNGSG010000000
VERSION     DBNGSG010000449.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4391141"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="AC158Q_11690"
     CDS             <1..433
                     /locus_tag="AC158Q_11690"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014796.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HIR3340478.1"
                     /translation="NAAQAWNHTAFWNSLKPGGGGEPGGEIAVRLHEAFGSYGGFRET
                     FSAAAVGQFGSGWAWLVLTGRRLAVINTPDAVNPLVWGQAPLLACDVWEHAYYLDYQN
                     RRKAFVEAFLDHLVNWEYVASALSEAEAQQAQPARAAAGRR"
     gene            complement(521..1921)
                     /locus_tag="AC158Q_11695"
     CDS             complement(521..1921)
                     /locus_tag="AC158Q_11695"
                     /inference="COORDINATES: protein motif:HMM:NF015722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HIR3340479.1"
                     /translation="MPEAGSDRQLLTILFADAAGYSRLMAAQERGTLDTLKEYRACFR
                     EHVARSKGRVIDTAGDSILAVFTSPTAAVECAVAIQDELAQRNRNLPPRRSMEFRVGI
                     NLGDVITEGGAVYGDGVNVAARLEGLAESGGIMISGAVYELVRDKVDHLFVYEGRRRV
                     KNISTPVAVYAVVPGHERASFLEKARRRKRQVVAAGAVAGALALAGVWYAIIPRGIAP
                     SGEILPLRIFRDCPECPELVQIPPGAYERGSRPGEPGHFDSEGPVTRVTIKRSFAIGR
                     YPVTFGEWDRCVSAGACKHRPNDRGWGRGTGPVFYVSWNDTLDYLGWLKVQTGKTYRL
                     PSEAEWEYAARAGASTAYPWGDSAGQKMANCKGCSDGASDRTTPVGSFPPNRFNLFDM
                     HGNVWQWVADCWNGSYAGAPADGSPRLSGECEKAAVRGGAWGLAPEDVRSARREGDSK
                     DLRSGRRGFRIARDLP"
     gene            complement(2051..2908)
                     /locus_tag="AC158Q_11700"
     CDS             complement(2051..2908)
                     /locus_tag="AC158Q_11700"
                     /inference="COORDINATES: protein motif:HMM:NF024921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR3340480.1"
                     /translation="MNTTMNRHGYLSGSSRNRVLGLMLGCALAGAAYGQPADRITIDG
                     STGMMPLVSALARAYHERNPGVTIEIGKGLGTKARIKALAEGGIDIAMASHGLVVEDI
                     VKQGMAVQEIAKVAVVFGVNAAVPVKTLGDSEICDLYSRRVVFWKNAGGADIAVAPMT
                     RPDSEVDTEVVRARVACLKPMQMPEGVRTMSKSGDMARALANTAGAVGMTTMTVVEQS
                     QSKIRPIWLNGVEPSAENVRSGAYSLTRDSFLVTKATPAPAVARFLEFVRGPDGKRVI
                     SANGAVPLD"
     gene            3108..>3473
                     /locus_tag="AC158Q_11705"
     CDS             3108..>3473
                     /locus_tag="AC158Q_11705"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3340481.1"
                     /translation="MTKEIRPDSLTGAELRALSGQGTVKTFQKHTVIVSEGDETDSLY
                     VVLSGRVRVFVADESGKEVVLGTQGAGEYFGEMVLDGGPRSASVMTLEPSRFLVIPKN
                     KMRDFLRSQPDFSIRLIEKL"
BASE COUNT          577 a         1188 c         1120 g          588 t
ORIGIN      
        1 caacgccgcg caggcctgga atcacaccgc cttctggaac agcctgaagc cgggcggcgg
       61 cggcgagccc gggggcgaga tcgccgtgcg gctgcatgag gccttcggca gctacggcgg
      121 gttccgcgag acgttctccg ccgccgcggt gggccagttc ggaagcggct gggcgtggct
      181 cgtgctgacc ggccgcaggc tcgccgtgat caacacgccg gacgccgtga acccgctggt
      241 ttggggccag gcgccgctgc tcgcgtgcga cgtgtgggag cacgcctact atctcgatta
      301 ccagaaccgg cgcaaggctt tcgtcgaggc tttcctcgac catctcgtca actgggaata
      361 cgtcgcgtcg gcactgtccg aggcggaggc gcagcaggcg caacccgcgc gcgccgccgc
      421 cggccggcgc taggcgtccg gttactggcc acgctctgtc gcgaatcgcc tgcttcgcgg
      481 attggcgact ttcgaggcaa agctttaagc cggattcagt tcaaggcagg tcccgggcaa
      541 tgcgaaagcc ccggcggccg ctgcgcaggt ccttgctgtc gccctcgcgg cgggcggagc
      601 gcacgtcctc cggagcgagc ccccaggcgc cgccgcgaac cgccgccttc tcgcattccc
      661 cgctcaaccg gggcgagcca tcggcgggcg cgccggcata ggacccgttc cagcagtccg
      721 cgacccattg ccagacgttt ccgtgcatgt cgaacaggtt gaaacgattc ggcggaaacg
      781 atcccaccgg cgtggttcga tcgctggcgc cgtccgagca gcctttgcag ttggccattt
      841 tctggcccgc tgaatcgccc cacggataag cggtgctggc gccggcccgc gcggcgtatt
      901 cccactccgc ttccgaaggc aggcggtagg tctttcccgt ttgcaccttg agccaaccga
      961 gataatccag ggtgtcgttc cagctcacat agaacaccgg cccggtgccc cggccccagc
     1021 ccctgtcgtt gggcctgtgc ttgcaggcgc ccgcgctcac gcagcggtcc cactcgccga
     1081 aggtcacggg gtagcgcccg atggcaaagc tgcgcttgat cgtgacgcgg gtgaccggcc
     1141 cctccgaatc gaaatggccg ggttcgccgg ggcgggagcc gcgttcgtat gcgccgggcg
     1201 gtatctggac gagctccggg cattccgggc agtcgcggaa tatgcgcaag ggaagtatct
     1261 ctccggaagg cgcgatgccg cggggaatga tcgcgtacca cacccctgcc aatgccaggg
     1321 cgccggcaac ggcgcccgcc gccacgactt gccgcttgcg ccggcgggct ttttccagga
     1381 aggacgcgcg ctcgtgcccg ggcaccacgg catagacggc gaccggcgtg gagatattct
     1441 tgacgcgccg gcggccctcg taaacgaaca ggtgatcgac cttgtcccgg accagctcgt
     1501 acaccgcgcc cgagatcatg atgccgccgc tctcggccag cccttccagg cgcgcggcga
     1561 cgttgacgcc atcgccatag acggcgccac cctcggtgat cacgtcgccg agattgatgc
     1621 cgacgcggaa ctccatcgac cggcgcggcg gcaggttacg attgcgttgc gccagctcat
     1681 cctgtatcgc cacggcgcat tcgacggccg cggtcgggct ggtgaaaacc gccaggatgc
     1741 tgtcgccggc ggtatcgatc accctgccct tcgaccgcgc gacatgctcg cggaagcacg
     1801 cgcggtactc tttcagggtg tccagcgtgc cgcgttcctg ggccgccatg agccggctgt
     1861 aacccgcggc gtcggcgaac aggatggtga gcaactggcg atctgaaccc gcctccggca
     1921 ctgcgccctc ccgcaagtga cgagcaaggc atagtacatg cctcgacccg aataaaacca
     1981 caatagcgga ttgcgcacgc gaaaaaaagc ggggaccggc caacagcccg gcccccgctt
     2041 gaacggcaga tcagtccagc ggcaccgcgc cgtttgcgct gatgacgcgc ttgccatcgg
     2101 ggccgcgaac gaattccagg aaccgggcaa ccgccggggc cggggtcgcc ttggtgacca
     2161 ggaacgagtc gcgcgtgagc gagtaggcgc cgctgcgcac gttttccgcg ctgggctcca
     2221 cgccattcag ccagatcggc ctgatcttcg actggctctg ctccacgacc gtcatcgtcg
     2281 tcatgccgac cgcgccggcc gtattcgcca gcgcgcgagc catatccccg gatttggaca
     2341 tcgtacgcac gccctccggc atctgcatcg gtttcaggca ggcaacgcga gcgcgcacga
     2401 cttcggtatc gacttcgcta tcgggcctcg tcatcggggc cacggcgata tcggcacctc
     2461 ccgcgttctt ccagaacacc acgcggcgcg aatagagatc gcagatttcg ctgtccccga
     2521 gcgtcttgac cggcaccgcg gcattcacgc caaagacgac cgcgaccttg gcgatctcct
     2581 gcaccgccat gccctgcttg acgatgtcct cgacgaccag accgtggctc gccatcgcaa
     2641 tgtcgatgcc gccctcggcg agcgccttga tgcgcgcctt ggtgccaagg ccttttccga
     2701 tctctatcgt cacgccggga ttccgctcgt ggtaggccct ggcgagcgcg gagaccagcg
     2761 gcatcatgcc ggtggatccg tcgatggtga tccggtccgc cggctggccg taagccgccc
     2821 cggccagcgc gcaaccgagc atcagtccca gcacgcggtt acgggaagaa ccggataagt
     2881 atccgtgcct gttcatcgtg gtattcatag cccctccttg agttgagttc atgaaaagcg
     2941 gtgctgcacg tgcatcctga tcccgggcaa agcggcggtc tgcagcgcat tgcgcacaat
     3001 ttctgtgggt tatttacagt tttggcgcac gcggctccgg gccggccggc gcgtctatgg
     3061 actcactcat ggccacgccc tagaatcagt gcggatccgg atgcgccatg acgaaagaaa
     3121 tccgccccga cagcctgacc ggggcggaac tgcgtgccct gtccggccag ggcacggtca
     3181 agacatttca gaagcacacc gtcatcgtca gcgaaggcga cgagacggac tcgctgtacg
     3241 tggtcctgtc cgggcgcgtc agggttttcg tcgcggacga gtcgggcaag gaagtcgtcc
     3301 tgggcaccca gggagcgggc gaatatttcg gcgagatggt cctggacggc gggccgcgtt
     3361 cggcttccgt catgacgctg gaaccgtcgc gcttcctggt cattcccaag aacaagatgc
     3421 gggacttcct gcgaagccag ccggatttct ccatccggct catcgagaag ctc
//
LOCUS       DBNGSG010000450         1433 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4731827, whole genome shotgun sequence.
ACCESSION   DBNGSG010000450 DBNGSG010000000
VERSION     DBNGSG010000450.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4731827"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="AC158Q_11710"
     CDS             <1..437
                     /locus_tag="AC158Q_11710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LeuD/DmdB family oxidoreductase small subunit"
                     /protein_id="HIR3340482.1"
                     /translation="SKYHPGRDNAYVAQHAFEQLAPGFAARFAKGGVIVAGKNFGNNS
                     SREQAVHILRLMGVAGIAAKSFGRQFFRNCINNGMPAVECDLTGIADGDEIELDLAAG
                     RVAVAARGIMREVPPLPPAMQAILASGGLIPFLKAHPGWDAV"
     gene            569..>1433
                     /locus_tag="AC158Q_11715"
     CDS             569..>1433
                     /locus_tag="AC158Q_11715"
                     /inference="COORDINATES: protein motif:HMM:NF024771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR3340483.1"
                     /translation="MKIEKVEVFGVAVPLIGEYKNAYLSKTVQKSAVVRITAGGMAGL
                     GNIDPTPGYSKEQTPEHLEVLRGRLAPLLIGMEATNVHAVLAKIEPAVKGFLDSKAAI
                     EMACVDLAARIAGIPVHAYLGGAVKDRLLFNAWIGILPPDEAAKETLGWKNKGFRSAK
                     IKVGGNIEADRDRVKAVREAVGPDFQLRIDANAGYDADTSIKLARMVAPFKLQLFEQP
                     VPDHDIAGMARVRKEANAVGLPIMADESVLDHASLIRIIKMDAADIVKVKVMKQGGFL
                     NTRRMIATAEAA"
BASE COUNT          277 a          424 c          487 g          245 t
ORIGIN      
        1 cgagcaagta ccacccgggc cgggacaacg cctacgtggc gcagcatgcc ttcgagcagc
       61 ttgcgcccgg tttcgctgcg cgcttcgcga agggcggcgt gatcgtggcc gggaagaatt
      121 tcggcaataa ctcctcccgc gagcaggcgg tgcacatcct gcgcctgatg ggcgttgcgg
      181 ggatcgcggc gaagtccttc gggcggcaat tcttccgcaa ctgcatcaac aacggcatgc
      241 cggcggtgga gtgcgatctc accggcatcg cggacggcga cgagatcgag ctggacctgg
      301 ccgcgggccg cgtcgcggtt gccgcgcgcg gcatcatgcg cgaagtgccg ccgctgcccc
      361 cggcgatgca ggccatactg gcgtcgggcg ggctgatccc gttcctcaag gcgcatcccg
      421 gctgggatgc tgtttgagag gcgtgaatgg tgattggtga ttagtgaatg gatgaaaccg
      481 gattttctaa cagcgcaact tgaatccaat caccattcac cattcacgca tttacgtaac
      541 tgattcgttc gacatttcga gagcgcggat gaaaatcgag aaagtggaag tgtttggcgt
      601 ggcggtgccg ctcatcggcg agtacaagaa cgcctatctt tccaagactg tccagaaaag
      661 cgctgtcgtg cgcatcaccg cgggcggcat ggcggggctc ggcaacatcg accccacgcc
      721 cggttattcc aaggagcaga cgccggagca tctcgaggtg ctgcgcggca ggctcgcacc
      781 gctgctgatc ggaatggagg cgaccaacgt tcacgccgtg ctggcgaaaa tcgagcccgc
      841 ggtgaagggt ttcctggatt ccaaggcggc gatcgagatg gcctgcgtgg acctcgccgc
      901 gcgcatcgcg ggcataccgg tgcatgccta cctgggtggc gcggtgaagg acaggctgct
      961 gttcaacgcc tggatcggca tcctgccgcc ggacgaggct gcgaaggaaa cgctgggatg
     1021 gaagaacaag ggctttcgct ccgccaagat caaagtcggc ggcaacatcg aggccgaccg
     1081 cgatcgcgtg aaggcggtgc gcgaagcggt ggggcccgat tttcagctgc gcatcgacgc
     1141 caacgcgggc tacgacgcgg acacctcgat caagctcgcc aggatggtgg cgccgttcaa
     1201 gctgcagttg ttcgagcagc cggtgcccga ccatgacatt gccggcatgg cgcgggtgcg
     1261 caaggaagcg aacgcggtag gcctgcccat catggcggac gaatcggtgc tcgaccatgc
     1321 gagcctgatc cgcatcatca agatggatgc ggccgacatc gtcaaggtca aggtgatgaa
     1381 gcagggcggc tttctcaata cccggcgcat gatcgcgacc gccgaggcgg ccg
//
LOCUS       DBNGSG010000451         2920 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2355169, whole genome shotgun sequence.
ACCESSION   DBNGSG010000451 DBNGSG010000000
VERSION     DBNGSG010000451.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2355169"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="AC158Q_11720"
     CDS             <1..451
                     /locus_tag="AC158Q_11720"
                     /inference="COORDINATES: protein motif:HMM:NF048150.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3340484.1"
                     /translation="AAPAGNAWPTGDIGHLDDEGYLHITGRKKDCFISSYGRNVSPEW
                     IEAALTLEPAIAQAWATGEARPWVAAVITPAPGQSDDAVNAAVARANRALPGYAQVRN
                     WIASSESFSCSNGELTANGRLRRAALFARYQAAIDALYREETHALSR"
     gene            435..1118
                     /locus_tag="AC158Q_11725"
     CDS             435..1118
                     /locus_tag="AC158Q_11725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HIR3340485.1"
                     /translation="MRFHDELLQATERERAELLAIPFIRDGAAGRLTRADYVAFLSEA
                     YHHVKHTLPLLMACGAKLPARLEWLRTAMAEYIKEETGHEQWILDDIRACGADAEAVR
                     AARPGLPAELLVAYAYDVIERVNPVGFLGMVLVLEGTSTAVATRAAGALMTSLKLTPA
                     AFTYLTSHGELDVGHTRFFATLVDRLDQTGDREVLVHCAKVFYKLYGDVFRALDRGRV
                     KDQARGIAA"
     gene            1115..1927
                     /locus_tag="AC158Q_11730"
     CDS             1115..1927
                     /locus_tag="AC158Q_11730"
                     /inference="COORDINATES: protein motif:HMM:NF006565.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3340486.1"
                     /translation="MNIAGSRILLTGATGGIGRALALELARRGARLALAGRDAARLAL
                     LAAEIPGGGAPAAILALDLARPGSHITLVQRSLESLGGIDVLVNNAGRSYFGPFADQD
                     EAAMRELVEINLTAPLLLARAVLPHLIRRGTGCIVNIGSVFGAIGFPYNSAYSASKFA
                     LRGLSEALRRELSDTGVKVLYVAPRATATAMNGPAVRALMSETGTAVDTPEKVAIVIT
                     NALASDREEVTIGGPERIFSRLNALLPRLVDGALIKQGRMAARYLLESKIAK"
     gene            1975..>2920
                     /locus_tag="AC158Q_11735"
     CDS             1975..>2920
                     /locus_tag="AC158Q_11735"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019631.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HIR3340487.1"
                     /translation="MSNPAPTSNATLSRTTLTILTSLWLVALGNFPFWRSIWQAEGGL
                     RAGNIFFLLLLPLVVLAWVHLLLSLLTWGRATKAVLGAVLLLSAAVGYFEYTYGMVLD
                     QGMLASIVQTHTTEALDLMSWRLWEWLLIFGVLPSLLIARVRVVGRPWTRELGVKALG
                     MAAAAAFLSIILLVHYQHFASLLRNHHELRLMLVPSNITAALYGYLKRHLATPIVLEV
                     VGADATRAVAAAHTGKPTLTVLVVGETARAENFSLNGYPRLTSPELARQHVLSFSDVS
                     ACGTSTAVSLPCMFLDVGRAGYDDTLALRREGLLDVLQH"
BASE COUNT          463 a         1003 c          930 g          524 t
ORIGIN      
        1 ggccgcgcct gccggcaatg cctggccgac tggtgatatc gggcatctcg acgacgaggg
       61 ctatctccac attaccggcc gcaagaagga ctgcttcatc agctcgtacg gccgcaacgt
      121 ttcacccgaa tggatcgagg ccgcgctcac gctggagccg gctatcgcgc aggcctgggc
      181 gacaggcgaa gcgcgcccgt gggtggccgc ggtaattacc cccgcgccgg gccagtcgga
      241 cgacgcggtc aacgcggcgg tggcgcgcgc caaccgggcg ctgcccggct atgcgcaggt
      301 gcggaactgg attgcctcga gcgaatcgtt ctcctgcagc aacggcgagc tcacagccaa
      361 cggccggttg cgccgcgccg cgctcttcgc gcgctaccag gctgccatcg atgcccttta
      421 ccgcgaggaa acccatgcgc tttcacgatg aactgctgca ggccaccgaa cgggagcgag
      481 ccgagctgct ggccatcccg ttcattcgag atggcgccgc cggccgcctc acccgagccg
      541 actacgtcgc gtttctgagc gaagcctacc accacgtcaa gcataccctg ccgctgttga
      601 tggcctgcgg ggcgaagctg ccggcgcggc tcgagtggct gcgcaccgcc atggccgagt
      661 acatcaagga ggaaaccggg cacgaacaat ggatactcga tgacattcgt gcctgcggcg
      721 ctgacgccga ggccgtccgc gccgcgcgcc ccgggctgcc cgcggagctg ctcgtggcct
      781 atgcctacga cgtgatcgag cgcgtcaatc cggtcggttt cctcggcatg gtgctggtgc
      841 tggagggcac cagcacggcc gtcgcgacgc gcgcggcggg ggcgctcatg acgtcgctga
      901 agctgactcc cgcggccttc acgtatctca cttctcacgg cgagctcgac gtcggtcaca
      961 cccggttctt cgccacgctc gtcgaccggc tcgaccagac cggggaccgc gaagttctcg
     1021 tccactgcgc aaaggttttc tacaagctct acggcgacgt gttccgggca ctcgaccggg
     1081 gacgggtcaa agatcaggcg cgcgggatag cagcatgaac atcgccggca gccgcatcct
     1141 gttgaccggc gcaaccggcg gcatcggccg ggccctggca ttggagctgg ccaggcgcgg
     1201 cgcgcgcctc gcgctggctg gccgggatgc ggcccgtctc gccctgctgg ccgctgaaat
     1261 ccccggtggc ggcgcccccg ctgccatact tgccctcgac ctggcgcgtc ccggcagtca
     1321 tatcactctg gttcagcgct cgctcgagtc gctgggcggg atcgatgttc tcgtcaacaa
     1381 tgcgggtcgc tcgtatttcg gtccgttcgc cgaccaggat gaggcggcga tgcgtgagct
     1441 ggtggagatc aacctgactg cgccgctgct cctcgcccgc gcggtgttgc cgcacctgat
     1501 ccgccgcggg accggttgca tcgtcaatat cggctcggtg ttcggtgcca ttggattccc
     1561 gtacaactca gcctattccg cctccaagtt cgcgctgcgt gggttatcgg aggcgctgcg
     1621 gcgggaactc tccgacaccg gcgtgaaagt gctctacgtc gcgccccgcg ccaccgccac
     1681 cgcgatgaac gggcccgcgg tgcgcgcact gatgtccgaa accggcacgg ccgtggatac
     1741 gccggagaaa gtggcgatcg tcatcaccaa tgcccttgcc agcgaccggg aagaagtcac
     1801 catcggcggg cccgagcgga ttttctcgcg gttgaatgct ttgttgccgc gcctggtgga
     1861 cggcgcgttg atcaagcaag gtcgcatggc cgcccgctat ctgctcgagt caaaaatcgc
     1921 caagtaaacc ggccccccgc catgacagcc accccggccc cggtgcgaaa ctccatgtca
     1981 aaccctgcgc caacgagcaa cgccacgctc agccggacaa ccctcacgat actcacttcg
     2041 ttatggctgg tagcgcttgg gaattttccg ttctggcgct cgatctggca ggccgagggc
     2101 ggattgcgcg caggcaacat cttcttcctt ctgctccttc cactggtcgt gctggcctgg
     2161 gtccatctgc tgctgagcct gttgacctgg gggcgcgcca ccaaggcggt actgggcgct
     2221 gtgttgctgc tctcggcggc ggtgggctat ttcgaataca cctacggcat ggtgctcgac
     2281 cagggcatgc tcgcgagcat cgtgcaaacc cacacaactg aagcgctgga tctgatgagc
     2341 tggcgcctgt gggagtggct gctgatattc ggcgtgctgc cgtcgctgct gattgcccgg
     2401 gtccgggtgg tcggcaggcc ttggacacgc gagttgggcg tcaaggcgct gggcatggcg
     2461 gccgcagccg ctttcctgtc catcatcctg ctcgtgcact accagcactt tgcgtcattg
     2521 ctgcgcaacc accacgagct gcgcctcatg ctcgtgcctt ccaatatcac ggccgccctg
     2581 tacggctacc tcaagagaca cctggccacc cccatcgtac tggaggtcgt tggcgcagac
     2641 gccacacgcg cagtcgcggc agcgcacacg ggaaaaccga cgctcacggt gcttgtggtc
     2701 ggcgaaaccg cccgtgctga aaacttctcg ctcaacggat atccgcgcct gaccagcccc
     2761 gaactcgcgc gccagcacgt gttgagcttc tccgacgtca gcgcctgcgg cacgtccacg
     2821 gctgtttcgc tgccctgcat gtttctggat gtcggccgcg caggttacga tgacacgctc
     2881 gccctgcgcc gcgaagggct gctcgacgtg ctgcagcacg
//
LOCUS       DBNGSG010000452        10414 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2015095, whole genome shotgun sequence.
ACCESSION   DBNGSG010000452 DBNGSG010000000
VERSION     DBNGSG010000452.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 10414)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10414)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10414
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2015095"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /locus_tag="AC158Q_11740"
     CDS             <1..1113
                     /locus_tag="AC158Q_11740"
                     /inference="COORDINATES: protein motif:HMM:NF014073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR3340488.1"
                     /translation="LAEFALRFGPPEYTALLLMGLFILAYMSGGSMLKTLAMAAFGLM
                     IGMIGIDVMSGYTRFSFGVTELGDGVGIVPVAVGLFGVSEILLTAGAPAAQRVQRPKL
                     RDLVPSREEFRLSAGPIARGSVLGFLIGIIPGSAHIISSFVSYGIERRISKHPERFGK
                     GAIEGVAGPESANNAAASGAFVPMMALGIPTSPVTAVMIAAIMVHGILPGPMLIQSQP
                     ELFWGFVASMYVGNVVLLILNLPMVGLFVNLLRIPYAYLYPCILCFCILGTYSVANSL
                     IDVWILLAMGAIGYLLRKFGYDLAPVALGLVLAPMLELSLRQSLAMSAGDYRIFIERP
                     IAATLFAFGLVLVLFSLKPLFFKGKDWRSEVGLEKQ"
     gene            1224..2075
                     /locus_tag="AC158Q_11745"
     CDS             1224..2075
                     /locus_tag="AC158Q_11745"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR3340489.1"
                     /translation="MKFPWQLVAAAAALCAPLASSEDAVDRSGGPYVPTPPAVVEAML
                     NVAGVGPGDFVIDLGSGDGRIVLMAATRFKAGGMGVDIDPELVDAANASAQKQGVGGR
                     VKFVRQDVRAADLGQATVVTLYLLPGMMTNLRNKLLAELKPGARVVSHDFLFDQWRPD
                     RSVTVETQEKYDITGIWTSDVHLWIVPAPVAGAWRGKWSGTRSEEFKLDIKQGFQHFE
                     GRLARGGGTVGVRDGRIAGPTLTFTVPGQNGRRERHSAVVAEGRMSGEVRDGETVIAR
                     WNATRVP"
     gene            2072..3292
                     /locus_tag="AC158Q_11750"
     CDS             2072..3292
                     /locus_tag="AC158Q_11750"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3340490.1"
                     /translation="MTGRAPGFGFLVGALLAAQTVGTLATMTLPAVAPKVAEAYGISA
                     SLIGYQISLLAAFMLVSLTFGSQLSVRWGACRATQVALGLLAAGCLTAILPHAAFVFA
                     SAVALGLGYGLLSPPASQLLARFTPPARRNLVFSLKQTGVPIGGILAAAITPAIAVSF
                     GWRWALAGTALVLCALILLMQRSRAGWDDDRDPSVRFGARPFAGIATVWNHLALRLLS
                     LSGGCFVIVQICLQTFTVVLFVEQFGIGLVTAGIVLTASQAGGVFGRLFWGWIADVTR
                     NCFVALSVLAGVMISACLLVPALEAGWPVAVACVLFFVLGSTASGWNGAFMAEVARLS
                     PRREVSAATGGSLVFVNVGKMIGPIVFTNIYLLGGSYTLLFGMLALPAAIGLACVLAA
                     HHVTARAALAGVKT"
     gene            complement(3441..4106)
                     /locus_tag="AC158Q_11755"
     CDS             complement(3441..4106)
                     /locus_tag="AC158Q_11755"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR3340491.1"
                     /translation="MNPLPRLAATGLCSVIAAAASLAAGHAHADIYGFVDERGITHFS
                     NSPLDHRYYLFRRERKDAAVPGSNLVVTNVPYSQPRRAIQVNPAHRNEFGSMVSLAAK
                     LHGLDPALLHAVVTVESAYNPKALSRRGAAGLMQLMPATAKRYAVNDIWDPRQNLNGG
                     ARYLRDLLALFNNNLSLALAAYNAGEGAVIQHGNRIPPYTETRSYVPQVLQHYKLYAD
                     RYK"
     gene            4105..4455
                     /locus_tag="AC158Q_11760"
     CDS             4105..4455
                     /locus_tag="AC158Q_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340492.1"
                     /translation="MAVDSTTSGGRWYNGRRSDMRNLTVILLMLVCAPARAEMTCEQY
                     GAIAQETVRLRDQGASLSRLLADFERGELKQRLTAHEFQVVKDVIRASFNRLLSPVEV
                     VEACMQGGTLIPAR"
     gene            4678..5559
                     /locus_tag="AC158Q_11765"
     CDS             4678..5559
                     /locus_tag="AC158Q_11765"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR3340493.1"
                     /translation="MNRMFPVSRRRVVLALGALPLARFAGAADDVDSKRTGGPYVPTP
                     QVVVDEMLRIGRVGAGDFVVDLGSGDGIIVLTAAARLKARGFGVDIDPELVKLANAEA
                     KKRGVADRASFHVQDVFKADISKATVLTLYLLPGMMVDLRPKVFGELKPGARVVSHDY
                     HFGDWQPDDQYTWDVPEKEKVNGVPRATVYLWIVPARVAGRWRISVEAPANESYELTL
                     RQNFQNLDGTVAGGAVKGGKLTQSRLAGEEISFAFTVGGDRHRFKGRVAGEVMEGVVQ
                     LAGGKGGAKWTARRVSS"
     gene            complement(5653..7257)
                     /locus_tag="AC158Q_11770"
     CDS             complement(5653..7257)
                     /locus_tag="AC158Q_11770"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HIR3340494.1"
                     /translation="MLLHVILFISGGAVLALELLASRILTPYFGVSLYIWTGILSITL
                     VALAFGYWAGGKLAAGKIASGQAARLVRLYALMPAVSAFAIVASCLVYPYLFPRLAVS
                     DLVLGAFAACLTLLFVPLVAASAMNPLLIAIQLARADGRTGDAGAGKVFFVSTLGSVA
                     GVLVTAFALIPHVSNFSATLAVALVLALLSVAITVRPPVPLAARGRLGLIAAGAVIAA
                     GMMLSLADAYIGRMWPASFGGRTWQPEAKLSSLFGTVKILRSVPEADGRYARLYFQDG
                     LVQNTVGSDGRSLSFYTYALEALAYAYSPQPRSALVLGLGAGIVPMRLASRNVAVEAV
                     EIDPASLKAATRYFGFDPSRVRVHLADARTQLKRCDRAHDVVFVDLFHGDGTPDYLIT
                     RDFFVDLRRCLAPGGIAVFNTFADLEHPDAYAHLLATLRSELPFIALYRPDWPGATHI
                     NSFIVAAASALPAPQPVKPDYVPPQYADALYGMMTRPLMLNRGLLKDGEIVTDARNRA
                     MHDVAIGQIIYRRSVVGVLPAAFLVN"
     gene            7380..8315
                     /locus_tag="AC158Q_11775"
     CDS             7380..8315
                     /locus_tag="AC158Q_11775"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR3340495.1"
                     /translation="MNTAAVRCAAIAGALPAALFATLALAAEPRVPYVPTPQEVVESM
                     LETAKVGPGDYLIDLGSGDGRIVITAAKKHGARGFGVDLNPDRIKESSDNARRAGVAD
                     RVAFYQRNLFETDLAQASVITMYLLPRVNVELRPKLLELAPGTRLVSHDFDMGDWRPD
                     THLTVDAKDKYGGAGGKSDIYFWVVPARAGGTWQWQTAVAGKPQPYEIRLEQKYQVIT
                     GSVWVGGRTATLKEARLRGANIRFNFTAEVNGSPVNHEFSGKVGAEAIQGTVNLSGPR
                     IQAQLEWTARRPARPAAAGPVPIRAAQVSTRSVPN"
     gene            8312..9241
                     /locus_tag="AC158Q_11780"
     CDS             8312..9241
                     /locus_tag="AC158Q_11780"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR3340496.1"
                     /translation="MTRFRLTPGAAAQWAGVLLAACFAAGGAPAQDWGDTPYVQTPKN
                     VVDRMLEIAKVGPGDYVIDLGSGDGRMVITAAKERGARGFGVDLDRKLVALANRNAAK
                     AGVANRAVFYQRDLHATDVSAASVMSIYLLPEVNLMIRGRLLSTLGPGTRIVSHDYGM
                     GDWPPDYQTELAAPGKTVGIGERSKIFYWVVPGHAAGRWRWKLAADGKPVEFDLQIQQ
                     KFQVLEGTLAAGGRTGRIEKGRLEGEKIEFSAAVDAVRYEFSGRIFSHAIEGKVKLSR
                     GAARELPWSATRVEIWDPRHAALTTEQALKEMN"
     gene            9238..10086
                     /locus_tag="AC158Q_11785"
     CDS             9238..10086
                     /locus_tag="AC158Q_11785"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR3340497.1"
                     /translation="MRGARALALAACVFFCGRAGAADWGTLPDIPTPYLPSTSVAVDE
                     MLRLANVAPGDLVVDLGSGDGRMVIAAARDYGARGLGIEIDAKLVAASRDNARKAGVA
                     DRVAFRQGDVLAADYREATVVTLYLLPNLVDRLKPRLLELAPGTRIVAHDYGFSDWKP
                     DRRVVISKTYLLYRVPARVEGRWRLQAALPDSERDYEFEIQQKYQEISGGVRVQGGFL
                     PAFEARLEGDRISFVLVEDGVSHRYEGRVAGSLEIEGVVRSGTGRTLAEGRWRATRIV
                     SAGGEG"
     gene            10116..>10414
                     /locus_tag="AC158Q_11790"
     CDS             10116..>10414
                     /locus_tag="AC158Q_11790"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR3340498.1"
                     /translation="MSEAKRNPGWLIHYGAYFVAFLGALAAAPALAQPKGDVPYVPTP
                     QVTVDEMLRLAGVGPQDFVLDLGSGDGRIVITAARKFGARGLGVDLDFHLVIQSE"
BASE COUNT         1657 a         3309 c         3713 g         1735 t
ORIGIN      
        1 ctcgccgagt tcgcgctgcg cttcggcccg ccggaatata ccgcgctgct gctgatgggg
       61 ctgttcatcc tcgcctacat gagcggcggc tcgatgctga agacgctggc gatggcggcg
      121 ttcggcctca tgatcggcat gatcggcatc gacgtgatga gcggctacac ccgcttcagc
      181 ttcggcgtga ccgagctcgg cgacggggtg ggcatcgtgc cggtcgcggt ggggctgttc
      241 ggcgtctccg agatcctgct gacggcgggc gctcccgcgg cgcagcgggt gcagcggccg
      301 aagctgcggg acctggtccc gtcgagggag gaattccgcc tgtcggcggg gccgatcgcg
      361 cgcggcagcg tgctcggctt cctgatcggc atcattccgg gctcggcgca catcatttcg
      421 tccttcgtgt cgtacgggat cgagcggcga atttcaaagc acccggagcg cttcggcaag
      481 ggagcgatcg agggcgtggc cggccccgag tccgccaaca acgccgcggc cagcggcgct
      541 ttcgtgccga tgatggcgct gggcatcccg acgagcccgg tgacggcggt gatgatcgcc
      601 gccatcatgg tgcacggcat cctgccgggg ccgatgctga tccagagcca gccggagctg
      661 ttctggggtt tcgtggcgag catgtacgtc ggcaacgtgg tgctgctgat cctcaacctt
      721 cccatggtgg ggctgttcgt caacctgctg cggattccct acgcctacct gtatccgtgc
      781 atcctctgct tctgcatcct cggcacctat tcggtcgcga acagcctgat cgacgtctgg
      841 atcctgctcg cgatgggcgc catcggctac ctgctgcgca agttcggcta cgacctcgcg
      901 ccggtggcgc tcggcctggt gctcgcgccg atgctggaac tctcgctgcg ccagtccctc
      961 gcgatgagcg ccggcgacta ccgtatcttc atcgagcgcc cgatcgcggc gaccctgttc
     1021 gcgttcgggc tggtgctggt cctgttcagc ctgaaaccgc tcttcttcaa gggcaaggac
     1081 tggcgctccg aggtcggcct tgagaaacag tgatgagtga tgagcgatga gcgatgagtg
     1141 acgaattgtc ccgcacacgc ggggcctgct cccgtcactc gccacccgtc acgcccttca
     1201 gtttttcagg tagcattcgc cctgtgaaat ttccctggca gctggtagca gccgcggcgg
     1261 cgctgtgcgc gccgcttgca tcgtccgagg atgccgtcga tcgcagcggc ggaccgtacg
     1321 tgccgacgcc gcccgcggtg gtggaggcca tgctgaatgt cgccggagtc ggccccggtg
     1381 acttcgtcat cgacctgggt tcgggcgacg gccgcatcgt gctgatggcg gcgacccggt
     1441 tcaaggcggg cggcatgggc gtggacatcg acccggaact ggtggatgcc gccaatgcct
     1501 cggcgcagaa gcagggcgtc ggcggccgcg taaagttcgt gcggcaggac gtgcgcgcgg
     1561 ccgatctcgg ccaggccacc gtggtcacgc tctacctcct gccgggcatg atgaccaatc
     1621 tgcggaacaa gctgctggcc gaactgaagc ccggcgcgcg cgtggtgtcg cacgactttc
     1681 tgttcgacca gtggcggccc gaccgcagcg tcacggtcga gacccaggaa aagtacgaca
     1741 tcaccggcat ctggacttcc gacgtgcacc tgtggatcgt gccggcgccg gtagcgggcg
     1801 cctggcgcgg gaaatggtcg gggacgagaa gcgaggagtt caagctcgac atcaagcagg
     1861 gtttccagca tttcgaaggc aggctcgcgc gcggcggcgg cacggtcggc gtccgggacg
     1921 gccggatcgc gggcccgacg ctcacgttca ccgttccggg acaaaatggc cgccgcgagc
     1981 gccactcggc ggtggtagcc gagggccgga tgagcggcga ggttcgggac ggggagacag
     2041 tcatcgcgcg ctggaacgca acccgcgttc catgacgggt cgcgcgccgg gcttcgggtt
     2101 cctggtcggc gcgttgctgg ctgcgcagac ggtcggcacc ctggcgacca tgacgctgcc
     2161 tgcggtcgcc cccaaggtgg ccgaagccta cggcatcagc gcctccctga tcggctacca
     2221 gatcagcctg ctcgccgcct tcatgctggt ttccctcacc ttcggcagcc agctttccgt
     2281 gcgctggggc gcctgccgcg cgacccaggt ggcgctcggg ctgctggcgg cgggctgcct
     2341 taccgcgatc ctgccgcacg cggctttcgt gttcgcgtcc gccgtggcgc tggggctggg
     2401 ctacgggctg ctttccccgc cggcgtccca gctgctcgcg cggttcactc cgcccgcccg
     2461 gcgcaacctg gtgttctcgc tgaagcagac cggcgtgccg atcggcggca tcctcgcggc
     2521 ggcgattact ccggcgatcg cggtgagttt cggctggcgc tgggcgctgg cgggcaccgc
     2581 gctcgtgttg tgcgcgctga tactgctgat gcagcgctcg cgcgccgggt gggacgacga
     2641 tcgcgatccc tccgtgaggt tcggcgcgcg gccgtttgcc ggcattgcga ccgtctggaa
     2701 ccacctggca ttgcggctgc tgtcgctgtc gggcggctgt ttcgtcatcg tgcagatctg
     2761 cctgcagaca ttcacggtgg tgctgttcgt ggagcagttc ggcatcgggc tggtcaccgc
     2821 cggcatcgtg ctgacggcgt cccaggcggg cggtgttttc gggcgcctgt tctggggctg
     2881 gatcgccgat gtcacgcgca attgtttcgt ggcgctgtca gtgctggcgg gcgtgatgat
     2941 ctcggcctgc ctgctggtgc cggcgctcga ggcgggctgg ccggtcgcgg tggcgtgcgt
     3001 gctgttcttc gtgctgggct cgacggcgag cggctggaac ggcgcattca tggcggaagt
     3061 cgcgcggctt tcgccgcgcc gcgaagtctc cgcagcgacc ggcggctcgc tcgtcttcgt
     3121 caacgtcggc aagatgatcg ggccgatcgt tttcacgaac atctacctgc tgggtggcag
     3181 ctacacgctg ctgttcggga tgctggcgct gcccgccgcg atcggcctgg cgtgcgtgct
     3241 cgcggcgcac cacgtgaccg cccgcgccgc gctcgccggc gtcaaaacct agaacccata
     3301 aatcaaccgc caagacgcca agcccgccaa gcacggttaa atccggacgc gccgcaccgg
     3361 tttaactcca cgcgttcgaa cgggcgatga ttcgccgttc aacgcgcgga agttacctgg
     3421 cacctatcac ttatcactca tcacttgtac cggtcggcgt agagcttgta gtgctgcagc
     3481 acctgcggca cgtagctgcg ggtttcggta tagggcggga tgcggttgcc gtgctggatg
     3541 acggctccct cgcccgcgtt gtaggcggca agcgccaggc tgagattgtt gttgaacagc
     3601 gccagcaggt cgcgcaggta gcgggcgccg ccgttgagat tctgccgcgg gtcccatata
     3661 tcgtttacgg catagcgctt ggcggtcgcg ggcatgagct gcatcaggcc ggcggcgcct
     3721 ctgcgcgaga gcgctttcgg gttgtaggcc gattccaccg tgacgacggc gtgcagcagc
     3781 gccgggtcga ggccgtgcag cttcgccgcc agggagacca tggagccgaa ctcgttgcgg
     3841 tgggccgggt tgacctggat tgcccgccgc ggctgcgaat agggcacgtt cgtgacgacc
     3901 aggttcgacc ccggcaccgc ggcgtccttg cgttcccgcc ggaacaggta gtaacggtgg
     3961 tcgagcgggg aattggagaa gtgggtgatg cccctctcgt ccacgaagcc gtagatgtcg
     4021 gcatgggcgt gcccggccgc aagcgacgcg gccgccgcga tgacggagca aaggccggta
     4081 gcggcaagcc tcggtagcgg gttcatggcc gtggattcta ctaccagcgg aggacgctgg
     4141 tacaatggcc gccggtccga catgcgtaac ctgacggtca tcctcctgat gctggtttgc
     4201 gcgccggcgc gcgccgagat gacctgcgag cagtacggcg cgatcgcgca ggagaccgtc
     4261 cggctgcgcg accagggcgc ttcgctctcg cggctgcttg ccgatttcga gcgcggcgag
     4321 ctgaagcaac gcctcacggc gcacgagttt caggtcgtga aggacgtgat ccgggcaagc
     4381 ttcaacaggc tgctgtcccc ggtcgaggtg gtggaggcgt gcatgcaggg cggaacgttg
     4441 attccagccc gctgagaaaa aaatggacag gattaactga cgaattaccg taaacccgtt
     4501 aacaccataa atagacagga tttacaagat ttacaggatt aaggcggcag ggcgtatgtt
     4561 ggtttcagtt tgaatcttgt taatcctgtc tcttagtgat tttggcggtt ctaactgatt
     4621 ttcggaaacg tttgtttctg tgaatcctgt ctgtttcact tgattgagaa gagagccatg
     4681 aatcgaatgt ttcctgtgtc ccggcggcgg gtggtactcg cgctcggcgc gctgccgctc
     4741 gcgcggttcg cgggggccgc cgacgatgtc gacagcaagc gcaccggcgg gccgtacgtg
     4801 cccacgccgc aggtagtggt ggacgagatg ctgcgcatcg ggcgcgtcgg cgccggcgat
     4861 ttcgtggtgg acctcggctc gggcgacggc atcatcgtgc tgacggcggc ggcccggctg
     4921 aaggcgcgcg gcttcggcgt tgacatcgac cccgagctgg tcaagctcgc gaacgccgag
     4981 gcgaagaagc gcggtgtggc cgaccgggca tcgttccacg tgcaggacgt cttcaaggcc
     5041 gacatcagca aggcgaccgt gctcacgctc tatcttctgc cgggcatgat ggtggacctg
     5101 aggccgaagg tcttcggcga gctgaagccc ggcgcgcgcg tggtctccca cgactatcat
     5161 ttcggcgatt ggcaaccgga cgaccagtac acgtgggacg tcccggaaaa agagaaggtc
     5221 aacggcgtgc cgcgggcaac cgtatacctg tggatcgtgc cggccagggt tgccggccgg
     5281 tggcgcatca gcgtggaggc ccccgccaac gagagctacg agctgacgct gcgccagaat
     5341 ttccagaacc tcgacggaac cgtggcgggc ggcgccgtga aaggcggcaa gctcacgcaa
     5401 tcgcgcctgg ccggggagga gatctcattt gcattcaccg tgggcggcga ccgccatcgt
     5461 ttcaaggggc gagtcgcggg cgaggtcatg gaaggggtgg tgcagctcgc ggggggcaag
     5521 ggcggcgcga agtggaccgc ccggcgcgtc agttcctgac gcgcgacgag ggagtgtcga
     5581 ggaatgagtg tcgaggggaa aaaccgctga atggcttgtt gccgttactc ttcacccgac
     5641 acccgacacc cgtcagttga cgagaaacgc cgccggcagc acccccacta ccgagcggcg
     5701 gtagatgatc tgcccgatcg cgacatcgtg catggcgcgg ttgcgcgcat cggtcacgat
     5761 ttccccgtcc ttcagcagcc cccggttcag catcagcggc cgcgtcatca tcccgtagag
     5821 cgcatcggcg tactggggcg gcacgtaatc cggtttgacg ggttgcggcg cgggcaacgc
     5881 ggacgctgcg gcgacaatga agctgttgat atgcgtcgca ccgggccagt ccgggcggta
     5941 gagcgcgatg aacggcagct ccgagcgcag ggtcgcgagc aggtgcgcgt acgcatccgg
     6001 gtgttccaga tcggcgaagg tgttgaacac cgcgatgccg cccggcgcaa ggcagcgcct
     6061 caagtcgacg aagaaatcgc gcgtgatgag gtagtccggc gtgccgtcgc cgtgaaacag
     6121 gtccacgaac acgacgtcgt gggcgcggtc gcatcgtttc aactgcgtgc gcgcgtcggc
     6181 gagatgcacg cgcacgcggg aaggatcgaa gccgaagtaa cgcgtcgccg ccttgagcga
     6241 agccgggtcg atttccaccg cctcgaccgc gacgttgcgc gaggcgaggc gcatcggaac
     6301 gatgccggcg ccgaggccca gcaccagtgc cgagcgcggc tgcggactgt aggcataggc
     6361 gagcgcttcg agggcgtagg tgtagaacga aagcgagcgc ccgtcggagc cgaccgtgtt
     6421 ctgcaccagc ccgtcctgga agtagaggcg ggcgtagcgg ccgtcggctt cgggcacgct
     6481 gcgcaggatc ttcaccgtgc cgaagagcga gctcagtttt gcctcgggct gccaggtcct
     6541 gccgccaaag ctggcgggcc acatgcggcc gatgtaggca tcggcgagcg acagcatcat
     6601 gcccgcggcg atcaccgcgc cggcggcgat cagcccgagc cgtccgcggg cggcgagcgg
     6661 cacggggggc cgcaccgtga tggcgacgga cagcagcgcc agcaccagcg ccaccgcgag
     6721 cgtcgctgaa aaattgctga catggggaat cagggcaaac gcggtgacga gcacgccggc
     6781 aaccgaaccg agcgtgctta cgaagaagac cttgccggcg ccggcatcgc ccgtccgccc
     6841 gtccgcgcgc gccagctgga tcgcgatcag cagcgggttc atggcggaag ccgcgaccag
     6901 cgggacgaac agcagggtga ggcacgcggc gaacgccccc agcacgaggt cggagacggc
     6961 caggcgcgga aaaaggtagg gatagacgag gcaggacgcg acgatggcga aggccgagac
     7021 ggcgggcatc agcgcgtaga gccggacgag ccgcgcggcc tggccgctcg cgattttgcc
     7081 tgccgcaagt ttgccgccgg cccagtagcc gaacgcgagc gccaccagcg tgatcgagag
     7141 tatgccggtc cagatgtaga ggctgacgcc gaagtaaggt gtcaggatgc gcgaggcgag
     7201 cagctccagc gcgagcaccg cgccgcccga gatgaacagg atgacgtgca gcagcatgag
     7261 aagatgaagt ttcggttata atccgccact ttccggattc ggaatataac ccacctccgc
     7321 gcgcagcgca ggccggggtt ccaggggcgc cgcccgtgca gtccatatcg tgtcggatca
     7381 tgaatacggc cgccgtgagg tgcgccgcca tcgcgggcgc gctgccggcg gcgcttttcg
     7441 ccacactcgc cctggcagcg gagccgcgcg tgccgtacgt tcccacgccg caggaagtcg
     7501 tggagagcat gctggaaacc gctaaagtgg gtcccggcga ctacctgatc gacctgggct
     7561 cgggcgacgg gcgcatcgtg atcaccgcgg cgaagaagca tggcgcgcgc ggtttcggcg
     7621 tggacctcaa tcccgaccgc atcaaggaaa gcagcgacaa cgcgcggcgg gcgggcgttg
     7681 ccgaccgtgt cgcgttctat cagcgcaacc tcttcgagac ggacctcgcc caggccagcg
     7741 tcatcacgat gtacctgttg ccgagggtca acgtcgagct gcggccgaag ctgctcgaac
     7801 tcgcacccgg cacgcgcctc gtgtcgcacg atttcgacat gggcgattgg aggccggaca
     7861 cccacctgac cgtggatgcg aaggacaagt acgggggcgc cgggggcaag agcgacattt
     7921 atttctgggt cgtgccggcc cgcgccgggg gcacctggca gtggcagacg gcggtggcgg
     7981 gcaagcccca gccctacgag atcaggctgg agcagaagta ccaggtcatc acgggctccg
     8041 tctgggtcgg cgggcgcacg gccacgctga aagaggcgcg cctgcgcggc gcgaatatcc
     8101 gcttcaactt taccgccgag gtgaacggct cgcctgtcaa ccacgagttc tccggcaagg
     8161 tcggggccga ggccatccag ggcaccgtga acctgtccgg cccgcgcatc caggcgcaac
     8221 tcgaatggac cgcgcgccgc ccggcgcggc cggccgctgc cggccctgtc ccgattcgtg
     8281 ccgcgcaggt ttccacccgc agcgtgccca attgacccga ttccgcttaa cgcccggcgc
     8341 tgccgcgcaa tgggcggggg tcctgctcgc cgcctgcttc gccgcgggcg gcgcgcccgc
     8401 ccaggactgg ggcgacacgc cctacgtgca gacgcccaag aacgtggtgg accggatgct
     8461 ggagatcgcc aaggtcggac ccggtgacta cgtgatcgac ctgggctccg gcgacgggcg
     8521 catggtgatc accgcggcga aggaacgcgg cgcccgcggt ttcggcgtgg acctcgaccg
     8581 caagctggtg gcgctcgcca accgcaacgc ggcaaaagcc ggcgtggcga atcgcgcggt
     8641 gttctaccag cgcgaccttc acgccaccga cgtcagcgct gcctcggtca tgagcatcta
     8701 cctgctgccg gaagtgaacc tgatgatccg gggccgcctg ctctccacgc tcgggcccgg
     8761 cacgcgcatc gtttcgcacg attacggcat gggcgactgg ccgccggact accagaccga
     8821 gctcgcggcg ccgggcaaga ccgtcggcat cggcgagcgc agcaagattt tctactgggt
     8881 ggtgccgggg catgccgccg ggcgctggcg ctggaagctc gccgcggacg gcaagccggt
     8941 cgagttcgat ctccagatcc agcagaaatt ccaggtgctc gagggcacgc ttgccgccgg
     9001 cggccgcacc ggacgcatcg agaaggggcg gctggaagga gagaagatcg aattctcggc
     9061 cgctgtcgac gcggtgcgct acgagttctc cggccgcatt ttcagccacg cgatcgaggg
     9121 caaggtgaaa ttgtcccgcg gcgcggcgcg ggagctgccg tggtccgcga cgcgcgtcga
     9181 gatctgggat ccgcgccacg ccgcgctcac cacggagcag gcgttgaagg agatgaattg
     9241 aggggcgccc gggccctcgc gctcgccgct tgcgtgtttt tctgcggccg cgcgggcgcc
     9301 gccgactggg ggacgctgcc cgacataccg acgccctatc tgccctcgac gtcggtcgcg
     9361 gtggacgaga tgctgcgcct cgcgaacgtg gccccgggcg acctggtggt ggacctgggc
     9421 tcgggcgatg gcaggatggt gattgcggct gcgcgcgatt acggcgcgcg cgggctgggc
     9481 atcgagatcg acgcgaagct cgtcgcggca agccgggaca acgcgcgcaa ggcaggcgtc
     9541 gccgaccgcg tcgcgtttcg ccagggcgac gtgctcgccg ccgattaccg cgaagccacg
     9601 gtcgtcacgc tctacctgct gcccaacctg gtggacaggc tgaagccgcg gctgctcgag
     9661 ctcgcgcccg gcacccgcat cgtggcgcac gactacggct tctccgactg gaagcccgac
     9721 cgccgggtgg tgatttccaa gacctacctg ctctatcgcg tgccggcgag agtggaaggc
     9781 cggtggcgcc tgcaggctgc gctgcccgac agcgagcgcg actatgagtt cgagatccag
     9841 cagaagtacc aggagatcag cggcggagtg cgcgtccagg gcggtttcct gccggcgttc
     9901 gaggcgcggc tggagggcga ccgtatttct ttcgtgctgg tggaggacgg cgtgagccac
     9961 cgctacgagg ggcgcgtcgc gggcagcctg gagatcgagg gggtggtgcg ctccgggacc
    10021 gggcgcactc tcgccgaggg ccgctggcgc gctacgcgca tcgttagcgc cgggggcgag
    10081 ggttgacccg agtgatgagt gatgagtgat aagtgatgag tgaagccaag cgaaatccgg
    10141 gatggttgat tcattacgga gcgtatttcg tggcttttct gggagcgctg gcggcggcgc
    10201 cggcgctcgc ccagccgaag ggggacgtgc cgtacgtgcc gacgccgcag gtgacggtgg
    10261 acgagatgct gcggctcgcc ggcgtcggcc cgcaggattt cgtcctcgat ctcggctcgg
    10321 gcgacgggcg catcgtgatc accgccgccc gcaagttcgg cgcgcgcggg ctcggggtgg
    10381 acctcgactt ccatctcgtc atccagagcg agga
//
LOCUS       DBNGSG010000453         3178 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3542589, whole genome shotgun sequence.
ACCESSION   DBNGSG010000453 DBNGSG010000000
VERSION     DBNGSG010000453.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3542589"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="AC158Q_11795"
     CDS             complement(<1..522)
                     /locus_tag="AC158Q_11795"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR3340499.1"
                     /translation="MRPLFATIDSSALRHNLGVVRAHAPAAAVLAVVKADAYGHGLAR
                     AAAALSEAEGFALVELEAALRLRSAGYQKRIVLLEGFFDPGELAVLVEHGLATVIHSM
                     EQVAALEDLAADAKLDVLLKVNTGMNRLGLSPEQYARALERLRRNAGVAGITLMTHFA
                     NADDERGVAWQMER"
     gene            complement(579..941)
                     /locus_tag="AC158Q_11800"
     CDS             complement(579..941)
                     /locus_tag="AC158Q_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340500.1"
                     /translation="MVKGEEQKLLELFGRLGEEQQDRLIAFAEFLTAGTPDGAAPTAP
                     LAIPRPAGETVTMAIRRLTRTYPMLDRRRLMAETSQFMAQHALEGRAANEVIDDLEAV
                     FARHYNERHKSQGTRHKE"
     gene            complement(1057..1962)
                     /locus_tag="AC158Q_11805"
     CDS             complement(1057..1962)
                     /locus_tag="AC158Q_11805"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR3340501.1"
                     /translation="MADRRLQVFYTVARHLSFTKAADQLFMTQPAVTFQVKQLEEQFN
                     ARLFERNHGKIALTPAGRLVLDYAERILGLSEEMETRVGELTGATSGPLLLGASTTIA
                     EFILPRILGEFKAEHPLVRAHLTVGNSELIENRVADHTLDLGLIESPSHRPGLVGEIC
                     CEDELVLICAPAHKFAKAAGVKPQELAGEPFVSREPGSGTRDFMDQYLRGCKVAPEDL
                     DVVMELGSPEAIKGVVETGLGVSIVSRATVGKELRLGTLAAVPLEPRLIRAFSFVYPG
                     EKFRSKLLTTFVEFATTRMRRLAGG"
     gene            2320..>3178
                     /gene="lplT"
                     /locus_tag="AC158Q_11810"
     CDS             2320..>3178
                     /gene="lplT"
                     /locus_tag="AC158Q_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HIR3340502.1"
                     /translation="MPFGFYIIMAAQFFSSLADNALLVAAIALLIQMAAPEWMTPLLK
                     FFFTVSYVAFAAFVGAFADSMPKGRVMFITNTVKIAGCLMLFFFAWIATPGNASYGLV
                     LLAYGVVGLGAAAYSPAKYGILTEYLPHQKLVIANGWIEGLTVLSIIIGTVLGGALIA
                     PDVSAHLLAIDLPSIDTGIDSPPEAAILVIIAFYAIAAVFNLYIPDTGVDHRTPSKNP
                     LFLVQEFSHCVKLLWSDKLGQISLATTTLFWGAGATLQFIVIKWAAVALGYTLSQATM
                     LQGICAAGIA"
BASE COUNT          531 a         1073 c          987 g          587 t
ORIGIN      
        1 gcgttccatc tgccacgcca ccccgcgctc gtcatcggcg ttggcgaaat gcgtcatgag
       61 cgtgatgccg gccacgcccg cgttgcggcg gagccgctcg agcgcccgcg cgtactgctc
      121 cggcgagagc ccgagccggt tcatgccggt gttcaccttc agcagcacgt cgagcttcgc
      181 gtccgcggcg aggtcctcga gtgccgcgac ctgctccatg ctgtgaatca cggtggcgag
      241 cccgtgttcc accagtaccg ccagttcgcc cgggtcgaaa aatccttcga gcagcacgat
      301 gcgcttctga tagccggcgc tgcgcaatct cagcgcggcc tcgagctcga ccagggcaaa
      361 cccttccgcc tccgacagcg cggcggcggc acgcgcaagt ccgtggccat aggcatcggc
      421 cttgaccacc gccagcaccg ccgcggccgg cgcgtgcgcc cggaccacgc cgaggttgtg
      481 ccggagcgca gaggagtcga tggtggcgaa gagaggacgc atgcgctaaa ggcacaaggc
      541 aaaaggcaaa aggcaaaagt aagaccgggt ggcgcgcttc attccttgtg ccttgtgcct
      601 tgtgacttgt gcctttcgtt gtagtgccgc gcaaaaaccg cttcgaggtc atctatgacc
      661 tcgttcgcgg cgcggccttc cagggcgtgc tgggccatga actgcgaagt ctcggccatg
      721 agccgcctgc ggtcgagcat ggggtaagtg cgcgtgaggc gccgtatcgc catcgtgacc
      781 gtctcaccgg ccggtcgcgg gatggcgagc ggcgcggtcg gcgcggcccc gtccggcgtg
      841 cccgccgtca gaaactccgc gaacgcgatc agcctgtcct gctgctcctc cccgagccgc
      901 ccgaacagct ccagcagctt ctgctcctcg cccttcacca caaccccgcc aggaaataga
      961 caggattcac aagattcaca agatgattta caaaaacctg agcagccatg ctttagattc
     1021 aatcccgtaa atcttgcaaa tcctgtccac tgcgctttag ccgccggcaa gccggcgcat
     1081 gcgggtggtg gcgaactcga cgaacgtggt gagcagcttc gagcggaatt tctcgccggg
     1141 atacacgaac gagaacgcgc ggatcagtcg cggctcgagc ggcaccgccg ccagcgtccc
     1201 gagcctgagt tccttgccca ccgtcgcccg cgacacgatg gacacaccga gcccggtttc
     1261 gaccacgccc ttgatcgctt cgggactgcc gagctccatc accacgtcga gatcctcggg
     1321 ggcgaccttg cagccccgca ggtactggtc catgaagtcc cgcgtgcccg aaccgggctc
     1381 gcggctcacg aacggttcgc ccgccagctc ctggggtttc acacccgcag ctttcgcgaa
     1441 cttgtgcgcc ggcgcgcaga tcagcaccag ctcgtcctcg cagcagattt cgccgaccag
     1501 gccgggccga tgcgaaggcg actcgatcag gcccaggtcg agcgtgtgat cggcgacgcg
     1561 gttctcgatc aattcggagt tgccgaccgt gagatgcgcg cgcaccaggg gatgctccgc
     1621 cttgaactcg cccaggatgc gcggcaggat gaactcggcg atggtcgtgg aggcccccag
     1681 cagcagcggt ccgctggtgg cgccggtcag ctccccgacc cgcgtctcca tttcctcgga
     1741 caatccgagt atgcgctcgg cgtagtccag cacgagccgc cccgccggtg tgagcgcgat
     1801 cttgccgtgg ttgcgttcga acaggcgcgc gttgaactgc tcctcgagct gcttcacctg
     1861 gaacgtcacg gcgggctgcg tcatgaacag ctggtccgcg gccttggtga agctcaggtg
     1921 cctcgcgacg gtgtagaaaa cctggagtcg gcggtcggcc atcagggcag tgatgagtga
     1981 tgagtaatga gtgatgagtc gggctcgcgg cacgctcgcg ccactctcgc cagccgccac
     2041 gccatgcctc gctcctcact ggaaaagggt tgatattcaa ccacatttcg ggtgatctgt
     2101 ggtataaaac cgcagccgca gcacccaggg atgagggatg aaggatgagg gatgagggac
     2161 cacttcgcgg caccgcgccg gttagagcgc aagatgacga agtgattgcg gaatgtccgg
     2221 gctttgccct tccgcccaca gccgccgtct cctgctgata atgtcccgct gccccctttt
     2281 cccctttccc tttttctttt ttccttgatg taggcccgca tgcctttcgg cttctacatc
     2341 atcatggcgg cgcagttctt ctcgtcgctc gccgacaacg cgctgctggt cgccgccatc
     2401 gcgctgctga ttcagatggc ggcgccggag tggatgacgc cgctgctcaa gttcttcttc
     2461 accgtctcct acgtggcgtt cgcggcgttc gtcggcgcgt tcgcggactc gatgcccaag
     2521 ggccgcgtga tgttcatcac caacacggtg aagatcgcgg gctgcctgat gctgttcttc
     2581 ttcgcctgga tcgcaacacc cggtaacgcg tcctacggcc tggtccttct ggcctacggg
     2641 gtcgtcgggc tcggcgcggc ggcctactcg ccggccaagt acgggattct caccgagtac
     2701 ctgccgcacc agaagctggt gatcgcgaac ggctggatcg aggggctgac ggtcctctcc
     2761 atcatcatcg gcacggtcct cggcggagcg ctgatcgcgc cggatgtctc cgcgcacctc
     2821 ctcgcgatcg accttccgag catcgatacc ggcatcgact ccccgcccga ggcggcgatc
     2881 ctggtcatca tcgcgttcta cgcgatcgcg gcggtcttca atctctacat acccgacacc
     2941 ggcgtggacc acaggacgcc cagcaagaac ccgctgttcc tggtccagga gttctcgcac
     3001 tgcgtgaagc tgctgtggag cgacaaactg gggcagatct cgctcgccac cacgacgctg
     3061 ttctggggag caggcgcgac gctgcagttc atcgtgatca agtgggcggc ggtcgcgctc
     3121 ggctacacgc tgtcccaggc caccatgctg cagggcatct gcgccgcggg catcgccg
//
LOCUS       DBNGSG010000454         3593 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2863788, whole genome shotgun sequence.
ACCESSION   DBNGSG010000454 DBNGSG010000000
VERSION     DBNGSG010000454.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2863788"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="AC158Q_11815"
     CDS             complement(<1..713)
                     /locus_tag="AC158Q_11815"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3340503.1"
                     /translation="MMKRSVICLCLLASAMPVAHAQSYPSKPVRIVVGFAAGGPSDIV
                     ARLMAQQLTERLGRQVVVENRAGATGTIGAELVAKSPPDGHSLYLASQTTHAVAPYMY
                     SKVGYDPLKDFSTVVLVSHNPLLAVAHPSLGVKSIKELVAMAKRRPGDLNFATGGQGS
                     SPHMSVELLKSLLGINMVAIHYKGDGAAIGELVGGQVPIMFASIAGLLPHAKSGKLRG
                     IAITGAKRSAIAPEFPTIQ"
     gene            complement(779..2413)
                     /locus_tag="AC158Q_11820"
     CDS             complement(779..2413)
                     /locus_tag="AC158Q_11820"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008641242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HIR3340504.1"
                     /translation="MAASSAPDNAEFDFIVVGAGSAGCVLANRLSENGRHSVLLLEAG
                     GPANSINLKIPLMVAKVLNDERYTWQYQTEPQAHLNNKPQRWVRGRVIGGSGSINGNL
                     FVRGDPKEYDGWRDQGCAGWGYSDMLPYFKRLEDYPEGDAGVRGRGGPIHCTNLDRFD
                     PLSDAFVAACREAGHPQLADYNDGSYEGAYYLQYSTRDGWRSSSAAEYLKPARNRANL
                     TTVSDAPATRVLMDAKRATGVEYRRGDTSVTARARREVVLAAGPLVSPRLLEQSGIGN
                     SDILQKHGIAVVHHLPGVGENLRDHPSARISFECAKPITINDVLRNPLLKVREGLRWF
                     FKSEGLLTICSATSQVAWRCDPKSTQPDMAMLLLPLSGRDRYARTARYGLDPHPGFTF
                     GLKVLQPRSVGTLHVQSRDPLAQASMDPRYLSHEADVQLFIEGMRFARRIAQMPALAP
                     LVVRETRPGPEIQDDAGLLDYMKETIQTSWHMVGTCKMGIDAQAVVDPELKVRGINGL
                     RVVDSSIFPTIPSSNTNIPTIATGEKGADLLLAAAR"
     gene            2536..3480
                     /locus_tag="AC158Q_11825"
     CDS             2536..3480
                     /locus_tag="AC158Q_11825"
                     /inference="COORDINATES: protein motif:HMM:NF009327.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HIR3340505.1"
                     /translation="MKLQQLRYAWEVYRRGNHISAAAEALHTSQPGISKQIKLLESEL
                     GFEIFSRRRNRIVGLTEPGREVVSAAQRILTDVDNLRNLGEDFSSPTGGSLTIATTHT
                     QARYVLPKIIERFVRRHPAVRIGLRQGDPTQVCEFVEAGHADIAIGTETTRSFPNLVM
                     LPCFQLSRSVIVKRGHPLLKVKKLTLEEIAKYPIISYDPAYSGYWRVTEAFRKAGIEP
                     NVIFGAVDADVSKTYVEHGLGIAILTTIAFDATHDRGLRARDASHLFAPSTSYITLRA
                     NTYLRRFVYDFIKSVAPSLEPEIVKAALKNSKSVQKQN"
BASE COUNT          622 a         1197 c         1150 g          624 t
ORIGIN      
        1 tcctggatgg tggggaactc gggcgcgatc gcggagcgct tggcgccggt gatcgcgatg
       61 ccccgcaact tgccgctctt cgcgtgcggc agcagcccgg cgatgctcgc gaacatgatc
      121 ggcacctgcc cgccgaccag ctctccgatc gccgcgccgt cgcccttgta atggatcgcg
      181 accatgttga tgccaaggag gctcttcagc agctccaccg acatgtgcgg cgaggaaccc
      241 tggccgccgg tcgcaaaatt caagtcgccg ggacggcgct tcgccatcgc caccagctcc
      301 ttgatcgatt tgacaccgag cgaagggtgc gccacggcga gcagcggatt gtgggacacc
      361 agcacgaccg tcgagaaatc cttgagcggg tcgtagccga ccttggagta catgtacggc
      421 gccacggcgt gcgtggtctg gctggccagg tagagagaat ggccgtcggg gggcgacttg
      481 gccaccagct cggcgccgat ggtacctgtc gcgcccgcgc gattctcgac gacgacctgc
      541 cggcccagcc gctccgtgag ctgctgcgcc atcaggcgcg ccacgatatc ggacggcccg
      601 ccggcggcga accccaccac gattcgcacc ggcttcgacg ggtacgactg cgcatgcgcc
      661 accggcatcg ccgatgccag cagacacagg caaatcaccg atcgtttcat catgttggct
      721 ttcctccctg gttttatctg cgtttatcgg cgttcaccgg cggccgcatt ccgctcttct
      781 accgggcggc cgcgagcagc aagtcggccc ccttctcacc ggtggcgatc gtcggtatgt
      841 tggtattcga ggacgggatg gtcgggaaga tcgaggaatc caccacgcgc aggccgttga
      901 tcccgcgcac tttgagctcg gggtcgacca ccgcctgcgc atcgatgccc atcttgcagg
      961 tgccgaccat gtgccagctc gtctgtatcg tttccttcat gtagtcgagc agcccggcgt
     1021 cgtcctggat ttccgggccg ggccgcgtct cgcgcaccac cagcggcgcg agcgccggca
     1081 tctgcgcgat gcggcgggca aaacgcatgc cctcgatgaa gagctgcacg tcagcctcgt
     1141 gcgagaggta cctggggtcc atgctggcct gcgcgagcgg atcgcgcgac tggacgtgca
     1201 gcgtgccgac cgaacgcggc tgcagcactt tcagcccgaa cgtgaacccg ggatgcgggt
     1261 cgagcccgta gcgtgccgtg cgggcgtagc gatcccggcc ggacaagggc aatagcagca
     1321 tcgccatgtc gggctgcgtc gacttcgggt cgcagcgcca ggcgacctgc gacgtggccg
     1381 agcagatggt cagcagcccc tcgctcttga agaaccagcg cagtccctct ctcaccttga
     1441 gcagggggtt gcgcaggaca tcgttgatgg tgatcggctt cgcgcactcg aacgaaatcc
     1501 gcgcgctggg atggtcgcgc aggttctcgc ccacgccggg caggtggtgc acgacggcta
     1561 tgccgtgctt ttgcaggatg tcactgttgc cgatgccgga ttgctcgagc agcctgggcg
     1621 agaccagcgg ccccgccgcc aatacgactt cgcggcgcgc ccgcgccgtc acgctggtgt
     1681 cgccgcggcg gtactcgacg ccggtcgcgc gcttcgcatc catcagcacg cgcgtggccg
     1741 gggcgtccga aacggtcgtg agattcgccc gattgcgcgc gggcttcagg tactccgccg
     1801 ccgaactgct gcgccagccg tcgcgcgtgg agtactggag atagtaggcg ccctcgtagc
     1861 tgccgtcgtt gtagtcggcg agctgcgggt gccccgcctc acggcatgcc gcgacgaaag
     1921 cgtccgacag cgggtcgaac cggtcgaggt tggtgcagtg aatcgggccg ccgcgcccgc
     1981 gcacaccggc gtcgccctcg gggtaatcct cgaggcgctt gaagtacggg agcatgtccg
     2041 aatagcccca gccggcgcag ccctggtcac gccagccgtc gtactccttc gggtcgccgc
     2101 gcacgaaaag attgccgttg atcgagccgg atccgccgat gacgcgcccg cgcacccagc
     2161 gctgcggctt gttgttgaga tgggcttgcg gctcggtctg gtactgccag gtgtagcgct
     2221 cgtcgttcag gactttcgcg accatgagcg ggatcttgag attgatgctg ttcgccggcc
     2281 cgccggcctc gagcagcagc acgctgtgac ggccgttctc cgagagccgg ttggcgagca
     2341 cgcagcccgc ggatcccgcg ccgacgacga tgaagtcgaa ctcggcgttg tcaggagctg
     2401 atgatgcggc catgcggcag cccttcctca cttcgacgcg ctacggtcat gccatggaat
     2461 ttaacagccg cgagaataac ctgccagaac attgtattat tctagtagaa ccctgcgtta
     2521 tcctgaggat agcccgtgaa gctgcagcaa ctgcgctacg cctgggaagt ctaccggcgc
     2581 ggcaatcaca tctccgcggc ggccgaggcc ctgcacactt cgcagcccgg catcagcaag
     2641 cagatcaagc tgctggaatc ggagctgggt ttcgagattt tctcgcgccg gcgcaaccgc
     2701 atcgtcggcc tgaccgagcc gggccgggag gtggtttcgg ccgcccagcg catcctcacg
     2761 gacgtcgaca acctgaggaa cctcggcgag gacttctcct cgcccaccgg cggcagcctg
     2821 actatcgcga ccacgcacac gcaggcgcgc tacgtgctgc cgaagatcat cgagcgcttc
     2881 gtcaggcgcc acccggccgt ccgcatcggg ttgcgccagg gcgacccgac gcaggtctgc
     2941 gagttcgtcg aggccgggca cgcggacatc gccatcggca cggaaaccac gcgctccttt
     3001 cccaacctgg tcatgctccc ctgcttccag ttgagccgca gcgtcatcgt caagcgcggc
     3061 caccccctgc tgaaagtgaa gaaactgacg ctggaggaaa tcgccaagta ccccatcatc
     3121 tcctacgacc ccgcctacag cggctactgg cgggtcaccg aagcgttccg gaaagcgggg
     3181 atcgaaccca acgtgatctt cggcgcggtg gatgccgacg ttagcaagac ttacgtcgag
     3241 catggattgg gcatcgccat cctgactacg atcgctttcg atgcgactca cgacaggggg
     3301 ctgcgcgcgc gggacgcgag ccacttgttc gctcccagca cgtcctacat cacgctgcgc
     3361 gcgaacacct acctgcgccg tttcgtatac gatttcatca agtcggtcgc gccgagcctg
     3421 gagccggaga tcgtcaaggc agcattgaaa aactcgaagt ccgtacaaaa gcaaaattga
     3481 accgccaaat agcgttaaat ccggacgcct ggcgccggtt taactccgcg cgttcgaacg
     3541 gacggatagc cgtcgttcaa cgcgcgggat acgattacgg atcacgaatc acg
//
LOCUS       DBNGSG010000455        15473 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4392023, whole genome shotgun sequence.
ACCESSION   DBNGSG010000455 DBNGSG010000000
VERSION     DBNGSG010000455.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 15473)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15473)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15473
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4392023"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="AC158Q_11830"
     CDS             complement(<1..470)
                     /locus_tag="AC158Q_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340506.1"
                     /translation="MTNSRIMNHDSRIALSGGRFLLFRALVLGPFLENSGRAALAVIA
                     ISLGVALGVAVHLVNASAINEFELAARHLAGAADLVVRGPRAGFDEALYARIARLPGI
                     EAANPALELEVPLAGRNDSLKIVGFDPLRAAQVQPSLLPERSGMVLDLFDPGTVL"
     gene            complement(488..1153)
                     /locus_tag="AC158Q_11835"
     CDS             complement(488..1153)
                     /locus_tag="AC158Q_11835"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3340507.1"
                     /translation="MLQVRGVCKSFGGARPREVLRDISLEVRRGEYVAIIGESGVGKS
                     TLLNLIAGLDLPDRGGINLDGVQLESLDDTARTRLRRSHIGFVFQAFHLLPHLTAAQN
                     VALPLSLNGTGPLPAAQRVAAILSVVGLADAAGAYPRELSGGEAQRVAIARAMAHRPQ
                     LLLADEPTGNLDQESAAQVLRLLREQVKQEGGAGILVTHSALAAATADRVLVLTADGL
                     HPQ"
     gene            complement(1165..2394)
                     /locus_tag="AC158Q_11840"
     CDS             complement(1165..2394)
                     /locus_tag="AC158Q_11840"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/OsmC family
                     protein"
                     /protein_id="HIR3340508.1"
                     /translation="MSDGPAPIQFPGSLGAPLAARLDLPPGKPAAYALFAHCFTCSKK
                     SLASSRISAALTARGLAVLRFDFTGLGGSEGDFANTNFSSNVADLVAAAAWLRQHHQA
                     PKLLIGHSLGGAAVLAAAREIPEAAAVATIGAPFDPAHLKRLLAPALPEIEGAGEAEV
                     ELDGRKFRIRKQLLEDIGNRNNQEAIGNLRKALLIFHSPRDTTVSIDNAAQIFLAAKH
                     PKSFVSLDDADHLLTRKDDAVYVSAVLAAWASRYIGAPSMEARPMTADAPGIVTVAET
                     REGKFSQAVTVGKHHLRADEPAGAGGSDTGPSPYDLLLAGLGACTSMTIRMYADLKQL
                     PLDKVTVRLRHDKVHAQDCAECETKEGKIDRIDREVELHGRLDEAQRAKLLEIANKCP
                     VHRTLHSEVWIPTRLKV"
     gene            2582..3550
                     /locus_tag="AC158Q_11845"
     CDS             2582..3550
                     /locus_tag="AC158Q_11845"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3340509.1"
                     /translation="MGKRTRGLLVCIVLGLISSAVAQAQPYPNKPVRVVIPFGAGGST
                     DVLIRIVASRLPETLGQQVVIDNRTGAGSMIGTEIVAKSAADGYTLLGAGSPYAIVPN
                     LYKKVPYNVQKDFAPIMQIASQPYGLAVHPSLNVRSVKDLIALAKKEPGKHNYASSGQ
                     GGAMHLFQALFTNMAGIDIVHVPYKGSGPVRSDLLGGQVKIGCLGLSSIIGHHKAGQL
                     RIIAVTTAKRSPELPDIPAIAETLPGYDASLWTGFLVPAGTPAPVIKRLHGDITKLMQ
                     TADVRNAFKSAGTDVVATDPKAFGDFLRIELAKWGKVVRDLKLQVQ"
     gene            complement(3674..5263)
                     /locus_tag="AC158Q_11850"
     CDS             complement(3674..5263)
                     /locus_tag="AC158Q_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR3340510.1"
                     /translation="MKTVTASQLKNMLHDGGEIALLDVREAGQFGESHLLFATPLPYS
                     RLELDVVALVPRNSARIVLCDDDGKGTSAPAARRLQALGYTDVAVLEGGNRAWSGAGY
                     GLFKGVNVPSKLFGELVEHEYSTPRITAQELVRMKEANEDFVLFDGRPLHEHQKMTIP
                     GSTCCPNAELPYRVSSMVKDPRTKIIVNCAGRTRSILGAQTLINFGVPNPVYALENGT
                     QGWYLSDLKLEHGSMRRYPERIDEASLPTLKAAAGRLMDRFAVRSVTARQVEAWQKET
                     NRTTYLCDVRTPEEFATGTIPGAVHAPGGQLIQATDQWVGVRNARIVVIDGGEGVRAP
                     VVASWLAQLGCDPWVLEGGVKSGLKGVLAISTVLPDLAKLSAAELKRACDAGHCAVFD
                     LGGSMNFRKAHVPGSRWSTRARLAADASEENGPIVLVAEDAGVARLAAVELFEAGRQD
                     VKSLEGGLAAWTKAGYPTEASPDVPTDAECIDHLFFVHDRHAGNKEAMKQYLAWETGL
                     MAQLDGQDRTSFRIGPAQSRR"
     gene            complement(5319..6794)
                     /locus_tag="AC158Q_11855"
     CDS             complement(5319..6794)
                     /locus_tag="AC158Q_11855"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3340511.1"
                     /translation="MVQDAPPAGPPEPDASGATKPTFDALGLIPELLRAVADEGYREP
                     TPIQVKAIPLVLQRRDVMGAAQTGTGKTAGFTLPILQLFAPQASNSPSPARHPTRALI
                     LTPTRELAAQIEESVRAYSKYIPLRATVVYGGVDIGPQMKALHGGVEILVATPGRLLD
                     HLHQKSVNLSRVEILVLDEADRMLDMGFIPDIKRILAVLPKQRQNLLFSATFPEEVRR
                     LAKELLRSPVTVEVAPRNAPADLVTHQVHLVPLLKKRSLLVRLIRSRDMRQVLVFVRM
                     KRDANRLARQLQQDGIVAKEIHSDRTQAERMQALEEFKQGKVPVLVATDIAARGIDIE
                     ELPFVVNYELPHTPEDYVHRIGRTGRAGLQGEAISLVSPEEMKFLEDIERLLKRKLPR
                     ASTPDPAAEDRPAHRSREPRHERARERQGHERGAERHSHKPQPATPRPAQSGFDYTKP
                     YEPASSDQQARPAPAPAIRRGPARPTAALLGGSPAKREPHK"
     gene            7003..7623
                     /locus_tag="AC158Q_11860"
     CDS             7003..7623
                     /locus_tag="AC158Q_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIR3340512.1"
                     /translation="MNIVIEQQAAQRGGYPLILTLDTHGVPHRWISWQHACYYYSKDQ
                     VAWTTGAHAFTVYGGMNRITCARSSITASSIIAIKGKAMALKAFGQVPPLNNRELFHR
                     DHQVCAYCAHMLPVSRLTRDHVIPFSRGGKDSWMNTVTACRACNERKGNCTPEQAGME
                     LVYLPYVPNRAEYLILTNRRILADQMEFLAQHVPAQSRLRQGQKLA"
     gene            complement(7631..8200)
                     /locus_tag="AC158Q_11865"
     CDS             complement(7631..8200)
                     /locus_tag="AC158Q_11865"
                     /inference="COORDINATES: protein motif:HMM:NF018473.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HIR3340513.1"
                     /translation="MLTTFDAASVQCTAESGARRVALLELYTSEGCDSCPPADRWISS
                     LPQHGLNAERVVMLGFHVDYWNYLGWKDPYARAEYSARQRTASRRSGARVIYTLQLLL
                     NGRDYRRGAFTDDIDSRVRALNQEKPGPRIRLKMNTESPGVLLVQGTADVTDAAEPGD
                     ARAYLAIYENNLFSHSGDVLQALVIPNCR"
     gene            8364..8993
                     /locus_tag="AC158Q_11870"
     CDS             8364..8993
                     /locus_tag="AC158Q_11870"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR3340514.1"
                     /translation="MSQFFSIHPDNPQPRLIRRAVLIVRAGGVIVYPTDSCYALGCHL
                     GDKAAMERMRAIRQVDDRHHFTLVCRDLADIGAYARVDNRQFRLLKSATPGSYTFILK
                     AARELPKRLLHPSRKTIGLRVPGHRVVQALLSELGEPLLSSTLALPGDDLPLNDAQEI
                     RDRLEHQVELILDCGSCGVTPTTVVDLTGESPVITRQGKGAAAPFGLLA"
     gene            9045..10247
                     /locus_tag="AC158Q_11875"
     CDS             9045..10247
                     /locus_tag="AC158Q_11875"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIR3340515.1"
                     /translation="MFVNRVLSGMRPTGALHLGHYHGVLRNWIRLQHEYECLYFVADW
                     HALTTHYDTPDVIEQHVWDMVIDWLAAGVDPAQATLFIQSRVPEHAELHLLLSMVTPL
                     GWLERVPTYKDQQEKLADKDLATYGFLGYPLLQSADILIYRANQVPVGEDQVPHVEFT
                     REVARRFNHVFGREEGFEQKAEAAIKKLGAKRARLYRQLRAKFLEKGDAEALAAARAL
                     VEEAQNLPMGDRERLFGYIEGGGKVVLMEPQALLTGASKMPGLDGQKMSKSYNNTISL
                     REDADSIAKKIRTMPTDPARVKRSDPGDPEKCPVWQLHLVYSDDKRRQWVQEGCRSAG
                     IGCLECKQPVIDAVIEELKPIRERASQYLEDPTLVRSIIADGCEKARKLAQETLREVR
                     QAMGLDYS"
     gene            10439..11242
                     /locus_tag="AC158Q_11880"
     CDS             10439..11242
                     /locus_tag="AC158Q_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="segregation and condensation protein A"
                     /protein_id="HIR3340516.1"
                     /translation="MTEPVAANPIAMVYGQPVLEFPHDLYIPPDALEVFLDQFEGPLD
                     LLLYLIRKENINVLDIPMARLTAQYLEYVEMMQTRSLELAAEYLLMAAMLIEIKSRML
                     LPRPALGELEEDPRAELVRRLMEYEQMKLAAHRLNELPQAGRDFSLVQVLFEDVAAER
                     LPEVNTEDLRSAWLALLTRAAVNRHHRITREQLSVRDHMTRILRRLQEARYVDFLELF
                     DPAQGIAVLVVTFLAILELARESLIEVSQQAAYAPIYVKLKAGHLAAVE"
     gene            11255..11878
                     /gene="scpB"
                     /locus_tag="AC158Q_11885"
     CDS             11255..11878
                     /gene="scpB"
                     /locus_tag="AC158Q_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HIR3340517.1"
                     /translation="MNKTSSDLEHVKKVLEAALLVSQEPLPIAELKKLFDEEFASDVL
                     RRALDELRKDWQGRGVELSQVASGWRFRARPEYQPYLDRLSPQKPPRYSRAVLETLAI
                     IAYRQPVTRGDIEELRGVVVSANIIRALEARGWVEVVGHKEVPGRPELLATTKAFLDD
                     LNVGSLEELPPLDDLGSLVERAPVSLQPPAADAPEPEMVEEPRRAAA"
     gene            complement(12039..13178)
                     /locus_tag="AC158Q_11890"
     CDS             complement(12039..13178)
                     /locus_tag="AC158Q_11890"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR3340518.1"
                     /translation="MKFFPAVRAATLVGAALALNSYSASAQTFKTQEHIVRVVKLVEG
                     LEHPWGLAFLPDGRMLVTERPGRLRIVGKDGKLVSQAVSGLPAVAVHGQGGLLDVALH
                     PRFAENNLVYFSYAGRGEGGVGTEVARGRLTGNRLESVQILFRQLPKGPTGRHFGSRL
                     VFDREGYLYITLGDRGEDERAQKPADHAGSVIRLHDDGKTPIDNPFAGRPGWKPEKFT
                     LGNRNIQGAALHPQTGMLWTHEHGPQGGDEVNVIRAGINYGWPIITYGVQYFIGTKIG
                     EGTHKPGMAQPLHYWVPSIAPSGMAFYTGDKFPAWRGDLFVGALRDQMLVRLKLDGEK
                     VVKEERMLKNELGRIRDVRNGPDGFLYLLTDESNGVLARLEPADR"
     gene            complement(13186..13698)
                     /locus_tag="AC158Q_11895"
     CDS             complement(13186..13698)
                     /locus_tag="AC158Q_11895"
                     /inference="COORDINATES: protein motif:HMM:NF024258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR3340519.1"
                     /translation="MKICIVSDSHDHREPLAAAVAEAKALGAQAVLHCGDLVAPSTLH
                     AVIGLELPIHLIHGNNAGDLFYLSKWAQEPRNRLHYYGQDGSLDLAKRRIFMVHYPHY
                     ARALALTGDYDLVCNGHEHRAVIERIGNVKGGETLRIDPGTVAGVSAPATYAFGDLQT
                     LEFEIRQVPR"
     gene            13814..14650
                     /locus_tag="AC158Q_11900"
     CDS             13814..14650
                     /locus_tag="AC158Q_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340520.1"
                     /translation="MFTSNPFAMLSAFIPPAFMPTFVVIMVVLVVAGTLFDIVHKGSA
                     RYFFANWKESRNKGKQLGGGELASIAVQTAVVDVLTSGEFCNPRRRIAHLLTMYGFLI
                     YAITTAIMIFGYPTPATPTPAILPMLWWIGGVMVCVGGYWFWFFIRVDVAAEGNSPFR
                     VMHADLFILSLLASVTLGLIWAFLQTRGGAGASVFFGLYIIASMVLFGSVPWSKFAHM
                     FFKPAAAFEKRLSKANGTAENLPTLSRDDHEQQKRHSMELLRDAPMDMGLGIKREAPR
                     HY"
     gene            14696..15175
                     /gene="aprB"
                     /locus_tag="AC158Q_11905"
     CDS             14696..15175
                     /gene="aprB"
                     /locus_tag="AC158Q_11905"
                     /EC_number="1.8.99.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009973 - adenylyl-sulfate reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019418 - sulfide oxidation [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate reductase subunit beta"
                     /protein_id="HIR3340521.1"
                     /translation="MPTFVYMTRCDGCGHCVDICPSDIMHIDKTYRRAYNIEPNMCWE
                     CYSCVKACPQNAIDVRGYADFAPLGHSVRVQRDEQKGTIAWRIKFRNGTEKNFLSPIT
                     TKPWGTAIPKLADVPAPSQEMRDSQLLYSEPKFIRFDDGELHTLKASGLKMKKGVYY"
     gene            15175..>15473
                     /locus_tag="AC158Q_11910"
     CDS             15175..>15473
                     /locus_tag="AC158Q_11910"
                     /inference="COORDINATES: protein motif:HMM:NF013086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR3340522.1"
                     /translation="MAYKTIVEEGIDILVVGAGLGGSGAAWEARFWGQDKKIVIAEKA
                     NIDRSGAVAQGLYAINCYMGTRWGENKPEDHVRYARIDLMGLVREDLLFDMARHV"
BASE COUNT         2777 a         4833 c         5012 g         2851 t
ORIGIN      
        1 agcaccgtac cgggatcgaa gaggtcgaga accatgcccg agcgttccgg cagcagagag
       61 ggctgcacct gcgcggcgcg caacgggtcg aatccgacga tcttcaggct gtcgttgcgc
      121 ccggcgaggg ggacttcgag ttccaacgcc gggttggccg cctcgatccc gggcaggcgc
      181 gcgatccggg catagagcgc ctcgtcaaat cccgcgcgcg ggccgcgcac caccagatcg
      241 gctgcgcctg caaggtgccg ggcggcgagt tcgaattcgt tgatcgcgct ggcattgacg
      301 aggtgaacgg caaccccgag cgccactccc agcgaaatgg cgatcacggc gagcgcagca
      361 cggccggagt tttccaggaa cgggcccagc accagcgcgc ggaaaagcag gaaacgcccg
      421 ccggaaagcg cgattcgtga atcgtgattc atgattcggg aattggtcat cggtcccagc
      481 cgtcgtgtta ttgggggtgc agaccatcgg cggtgagcac caggacgcgg tccgcggttg
      541 cggcggccaa cgccgagtgc gtgacgagta tgccggcgcc gccttcctgc ttcacctgtt
      601 cgcgcagcag cctcagcacc tgcgcggcgg actcctggtc gaggttgccg gtgggctcgt
      661 cagcgagcag caattgcggg cggtgcgcca tggcccgcgc gatggccacg cgctgcgctt
      721 cgcccccgga cagttcccgg gggtaagcgc cggccgcgtc ggccagcccc accacggaca
      781 gtatcgcagc gactcgctgc gcggccggga ggggaccggt accgttgaga gacagcggca
      841 gggcgacgtt ctgggcggcc gtgagatgcg gcagcaggtg gaacgcctgg aacacgaacc
      901 cgatgtgact gcggcgcaac cgtgtccggg cggtatcgtc cagcgattcc agttggacgc
      961 catccaggtt gatgccgccg cggtcgggca ggtccagtcc tgcgatcagg ttcagcagcg
     1021 tggacttgcc gacgccggat tcgccgatga tggcaacgta ttcgccgcgc cggacttcga
     1081 gcgaaatgtc gcgcagcact tcgcggggcc tggctccgcc gaaggatttg cagacgccgc
     1141 ggacctggag catggcggct ggtctcagac tttgagccgt gtgggaatcc acacctccga
     1201 gtgcagagtg cggtgcaccg ggcatttatt ggcgatctcg agcagcttcg cgcgctgagc
     1261 ttcgtcgagc ctgccgtgga gctcgacctc gcggtcgatg cggtcgattt tgccctcttt
     1321 cgtttcgcac tcggcgcagt cctgggcatg gaccttgtcg tgcctgaggc gcaccgtcac
     1381 cttgtcgagc ggcagttgtt tcaagtcggc atacatgcgg atcgtcatcg aggtacacgc
     1441 gccaaggccg gcgagcagca gatcgtaggg ggaaggcccg gtgtcggagc cgcccgcgcc
     1501 ggccggttcg tccgcgcgca ggtggtgctt gcccacggtg acagcctggg agaacttgcc
     1561 ctcgcgcgtc tcggcgaccg tgacgatgcc gggcgcgtcg gccgtcattg gccgggcctc
     1621 catcgagggt gcgccgatat agcggcttgc ccatgcagcg agcaccgccg aaacgtacac
     1681 ggcatcgtcc ttgcgcgtga gcagatgatc ggcatcatcc agcgagacaa agctcttggg
     1741 gtgtttcgcc gccaggaata tctgcgcggc gttgtctatg ctcaccgtgg tgtcgcgcgg
     1801 cgaatggaag atgagcaggg ccttgcgcag attgccgatc gcttcctggt tgttgcggtt
     1861 gccgatatcc tcgagcaact gcttgcgtat cctgaacttg cgcccgtcca gctccacttc
     1921 ggcttctcct gcgccttcga tttctggcag ggctggcgcg agcagacgtt tcaagtgagc
     1981 gggatcgaaa ggcgcaccga tggtggcgac cgcagccgct tccgggattt cccgggccgc
     2041 ggcgagcacc gccgcaccgc cgaggctgtg cccgatcagc agctttggtg cctggtggtg
     2101 ttgccgcagc cacgcggcgg cggccacgag atccgcgacg ttggaggaga aattggtgtt
     2161 ggcgaaatcg ccttcgctgc cgccgagtcc ggtgaagtcg aagcgcagga ccgccaatcc
     2221 gcgcgcggtg agcgccgcgc tgatgcgcga ggacgcgagg ctttttttgg aacaggtgaa
     2281 gcaatgggca aatagggcat aggcagcggg tttgcccgga ggcaggtcga ggcgcgcggc
     2341 aagcggtgcg cccagcgagc cggggaattg gatgggggcg gggccgtcgc tcatggagtt
     2401 tcctcgcgcg gttttcgggc gatggtacca tgcgcctgcc gccccgagcg gggacaggcg
     2461 atgggttttc ttgggaattt ccgaaagcta ggatagacta acctgccgtc agaacattga
     2521 tcctgcccga ttacttgctg gcgtcggccg gggtcacggt ttagaacaac gaggaggact
     2581 catgggcaag agaacacggg gtttgctggt ttgcatcgta ctaggcctga tttcgtctgc
     2641 cgtcgcgcag gcgcagccgt atccgaacaa gccggtgcgc gtggtcattc cgttcggtgc
     2701 gggcgggtcg accgacgtgc tcatacgcat cgtcgcatcg aggctgccgg aaacactcgg
     2761 tcagcaggtg gttatcgata atcgcaccgg cgccggcagc atgatcggta ctgaaatcgt
     2821 ggcgaagtcg gccgccgacg gctacacgct gctgggagcc ggatcgccgt acgccatcgt
     2881 gccgaacctg tacaagaagg tcccgtacaa cgtgcaaaag gacttcgcgc cgatcatgca
     2941 gatcgccagc cagccctacg gcctggcggt tcatccctcg ctcaacgtga ggtcggtcaa
     3001 ggacctgatc gcgctggcca agaaggagcc cggcaagcac aactacgcct cgtcgggtca
     3061 gggcggcgcc atgcacctgt tccaggcgct gtttaccaac atggctggaa tcgatatcgt
     3121 acacgtgccg tacaagggca gcggcccggt gcgcagcgac ctgctgggcg gccaggtcaa
     3181 gatcggctgt ctcggcctct cgagcatcat cggccaccac aaggcggggc agttgcgcat
     3241 catcgcggtg acgaccgcca agcgctcgcc ggagcttccc gacattccgg ccattgcgga
     3301 aacattgccg ggctacgacg cttcgttgtg gacgggcttc ctggtgccgg cgggaacccc
     3361 ggcgccggtc atcaagcgcc tgcacggcga catcacgaag ctgatgcaga ccgctgacgt
     3421 gcgtaacgcg ttcaagagcg cgggcacgga cgtggtcgcc acggatccga aagcctttgg
     3481 cgatttcctg cgcatcgagc tggccaagtg gggcaaggtc gtgcgggacc tcaagctgca
     3541 ggtgcagtaa accgggttgc ccgtttcgcc accgcagcgg gtgcaaccgg tgaatggctg
     3601 ccggcccggt cattcccgca agcggctgaa gccggcccat cgggccggct tttttctttg
     3661 atcgtaacgg ggcctagcgc cgggattgcg ccggaccgat ccggaaggaa gtcctgtcct
     3721 gcccgtcgag ttgggccatg aggccggttt cccaggccag gtactgcttc atcgcttcct
     3781 tgttgccggc gtggcggtcg tggacgaaga agaggtgatc gatgcactcg gcgtcggtcg
     3841 gcacgtcagg agaagcttcg gtcggatagc ccgccttcgt ccaggccgcc aggccgccct
     3901 cgagcgactt cacatcctgt cttcccgctt cgaacaattc gaccgcggcc agccgcgcga
     3961 cgccggcatc ttcggccacc agcacaatcg gtccgttttc ctcactcgcg tctgccgcca
     4021 ggcgcgcgcg cgtgctccag cggctgccgg gaacatgcgc cttgcggaaa ttcatgctgc
     4081 cgcccaggtc gaagacggca caatggcctg catcgcaggc cctcttcaat tcggcggcag
     4141 acagtttcgc gaggtcgggc aggacagtcg aaattgcgag cacgcccttg agcccggact
     4201 tcactccacc ctccagcacc caggggtcgc agccgagttg cgcgagccag ctggctacga
     4261 ccggcgcgcg cacaccctcg ccgccgtcga tcaccacgat acgtgcattg cgcacgccca
     4321 cccattggtc ggtggcctgg atcagctggc caccgggggc gtggacggcg ccgggtatcg
     4381 tccccgtggc gaattcctcc ggcgtgcgca cgtcgcaaag gtaggtggtg cgattcgtct
     4441 ccttttgcca cgcctcgacc tggcgggcgg tcacggagcg gaccgcgaag cgatccatga
     4501 gcctgcctgc cgcagccttg agcgtgggca acgatgcttc gtcgatccgc tcagggtatc
     4561 tgcgcatcga gccgtgctca agcttcaggt cggacaaata ccatccttgc gtgccgttct
     4621 ccagcgcgta aaccgggttc ggcacgccga agttgatcaa cgtttgcgca ccgaggatcg
     4681 agcgtgtgcg gccggcgcaa ttcacgatga tcttggtccg cgggtctttc accatgctcg
     4741 atacccggta aggcagttcc gcgttcgggc agcaggtcga gccggggatg gtcattttct
     4801 ggtgctcgtg cagagggcgg ccgtcgaaca gaacgaagtc ctcattcgcc tccttcatac
     4861 gcacgagttc ctgggccgta atgcggggcg tactatattc gtgctcgacc agttcgccga
     4921 agagcttgct cggcacgttg acgcccttga acaggccgta gcccgcaccg gaccatgccc
     4981 ggttcccgcc ctccagcacg gccacgtccg tgtaacccag cgcttgcagc cgtctcgcgg
     5041 cgggcgccga cgtgcctttg ccgtcgtcat cgcatagcac gatgcgcgct gagttgcgcg
     5101 gcaccagagc gaccacgtcc agctcgagac ggctgtaggg cagaggggtc gcaaagagaa
     5161 gatgggactc gccgaattgt ccggcttcgc gtacgtcgag caacgcgatc tcgccaccat
     5221 cgtgcagcat attcttgagc tgtgaggctg tgacggtttt catgtgaaga ggcaaggcag
     5281 gcgacgaaga acgagcaacg agaaacgagc agcggaagtt acttgtgggg ctcgcgcttg
     5341 gctgggctgc cgccgagcag cgcggcggtc ggtcttgccg gcccccggcg gattgccggc
     5401 gcgggcgcgg ggcgggcctg ctgatccgac gaggcgggct cgtacggttt ggtgtagtcg
     5461 aatcccgatt gcgcaggccg cggcgtggcg ggttgaggct tgtgactgtg ccgctccgcc
     5521 ccgcgctcgt ggccctggcg ctccctggcg cgctcgtgcc ggggctcgcg tgagcgatgc
     5581 gcaggccggt cttcggccgc agggtccggc gtggaggccc ggggcagctt gcgcttgagc
     5641 agccgctcga tatcttccag aaacttcatt tcttccgggc tgaccaggga aatcgcctcg
     5701 ccctgcagcc cggcgcggcc cgtgcggccg atgcggtgca cgtaatcttc cggggtatgc
     5761 ggcagttcgt aattgaccac gaacggcagt tcctcgatgt cgatgccgcg ggcggcgatg
     5821 tcggtcgcga ccagcacggg caccttgccc tgcttgaatt cctcgagcgc ctgcatgcgc
     5881 tcggcctggg tacggtcgct gtgaatctcc ttggcgacga tgccgtcctg ctggagctgg
     5941 cgcgcgaggc gattggcgtc gcgcttcatg cgcacaaaga ccagtacctg ccgcatgtcg
     6001 cgcgaccgga tgagtcggac cagcagcgag cgtttcttca gcagcggcac gaggtggacc
     6061 tggtgcgtga cgagatcggc cggcgcgttg cgcggcgcga cctcgaccgt caccggcgag
     6121 cgcagcagtt ctttggcgag cctgcgcact tcctcgggaa aggtcgcgga gaacagcagg
     6181 ttctgccgct gcttcggcaa taccgcgagg atgcgcttga tgtcgggaat gaagcccatg
     6241 tcgagcatgc ggtcggcttc atcgagcacc aggatttcca cgcgcgacag attgacgctt
     6301 ttctggtgca ggtgatcgag caggcggccg ggcgtcgcga cgaggatttc cacgccgccg
     6361 tgcagggctt tcatctgcgg gccgatgtcc acgccgccgt acacgaccgt tgcgcgcagc
     6421 ggaatgtact tgctgtaggc gcgaacgctt tcctcgatct gggcggcgag ttcgcgcgtc
     6481 ggcgtgagaa tcaacgcgcg cgtcgggtgc cgggctggcg aagggctgtt gctggcctgc
     6541 ggcgcgaaca actggaggat gggcagcgtg aagcccgccg tcttgccggt cccggtctgg
     6601 gctgcgccca tgacgtcccg ccgttgcagg acgagcggga tcgccttgac ctggatcggc
     6661 gtcggctccc ggtagccttc gtcggcgacc gcgcgcagca gctccgggat caaccccaga
     6721 gcatcgaagg tcggcttggt tgcaccggac gcgtccggct cgggcgggcc ggcaggaggg
     6781 gcgtcttgaa ccaatgtcac agtggccggc aacggccgct cgggcagtat ggaagcttat
     6841 tcacgggccc gcattatacc ttgctgcgct gcggcgcact acgcatgcct ggtgccggtt
     6901 acccccgtcc gatggctgta aatccctgaa actgccacaa attcaaaaat ctggtaaatt
     6961 tccaccttga aaaggtgaac tgggcaataa acgaccgaag tcatgaacat cgtcatcgaa
     7021 caacaagctg cgcagcgcgg cggctacccg cttatcctga cgctcgacac gcacggcgtg
     7081 ccgcaccgct ggatcagctg gcagcacgcc tgctactact attccaagga ccaggttgcc
     7141 tggacgacgg gggcgcatgc attcaccgtc tacggcggca tgaaccgtat cacctgcgcg
     7201 cgctccagca tcacggcctc cagcatcatc gccatcaagg gcaaggcgat ggccttgaag
     7261 gctttcggcc aggtgccgcc gctgaataac cgcgaattgt tccaccgtga ccaccaggtc
     7321 tgtgcctatt gcgctcacat gcttccggtc tcgcggctga cgcgcgatca cgtgataccg
     7381 ttctcgcgcg gcggcaagga cagctggatg aacacggtca cggcctgtcg cgcctgcaac
     7441 gagcgcaagg gcaattgcac tccggagcag gccgggatgg agttggtcta tcttccgtat
     7501 gtgccaaacc gcgcagagta cctgatcctc accaaccggc gcatccttgc cgatcaaatg
     7561 gaattcctgg cgcagcacgt tcctgcccag agccgcctgc gccagggcca gaagctcgcc
     7621 tgaatcgccg tcaacggcag ttgggaatta ccagcgcctg cagtacgtcg ccggaatgac
     7681 tgaagaggtt gttctcgtag atcgccaggt aagcccgtgc gtctccaggt tccgcagcat
     7741 ccgtcacatc ggccgtgcct tgcaccaaaa gcacgcccgg ggattccgtg ttcatcttca
     7801 gcctgatccg gggtcccggt ttttcctgat tgagcgcccg gacccggctg tcaatatcat
     7861 ccgtgaaggc gccgcggcgg taatccctgc cgttaagcag caactggagc gtgtatatca
     7921 cgcgcgcccc gctgcgccgg ctggcggtgc gctggcgcgc gctgtactcc gccctcgcat
     7981 aaggatcttt ccagcctagg tagttccagt aatcgacgtg aaagccgagc atcaccacgc
     8041 gctctgcgtt gaggccgtgc tgcggcaggc tgctgatcca gcggtcggcg ggcgggcagc
     8101 tgtcgcagcc ctcggacgtg tagagttcga gcagcgcgac gcgccgggca ccgctttccg
     8161 cagtacattg cacggacgcg gcgtcgaacg tcgtcagcat ggctatagcg gccagaaata
     8221 gtcgcaaccg gatcgtcaca acctatcccg tcaaatgggc tcaatggatt tacgcgtcgt
     8281 gaaagcacct ggattcgatc agcttatttg tatttgatgt tatacttcag taagcttctg
     8341 tattaacctg atttatatat agtttgtctc agtttttctc catccacccc gacaatccgc
     8401 agccacgact catccgccgc gcggtcctga tcgtgcgcgc cggcggtgtg atcgtttatc
     8461 caaccgactc ctgctatgcg ctcggttgcc atctcgggga caaggcggcc atggagcgca
     8521 tgcgcgccat ccgccaggtg gacgatcgcc accactttac gctggtgtgc cgtgatctcg
     8581 ccgatatcgg tgcttacgcg cgggtggaca accgccagtt caggctgctg aaaagcgcca
     8641 cgccgggcag ctacactttt atcctgaaag cggcgcggga gttgccgaag cggcttctgc
     8701 acccgagccg caagacgata ggtttgcgcg ttccgggtca ccgcgtggtg caggcgctgc
     8761 tctctgaact gggcgagccg ctgctctcgt cgacgttggc tctgcccgga gacgacctgc
     8821 ctcttaatga cgcgcaagag attcgagatc gcctcgagca ccaggtggaa ctcatactcg
     8881 actgcggctc gtgcggcgtt acgccgacga cggtcgtgga ccttaccgga gagtccccgg
     8941 tgatcacgcg ccagggaaag ggcgccgccg cgccgtttgg gctgctggca tgagcgcgtc
     9001 gcgtccggga cgcgggtcgc aacctgttag aattgcccga taatatgttc gtcaaccgcg
     9061 tactgtccgg catgcgtccg accggggcgc tgcatctggg ccattatcac ggcgttctca
     9121 ggaactggat ccggctgcag cacgagtacg agtgcctgta cttcgttgcc gactggcacg
     9181 ccctcaccac ccactacgac acgcccgatg tcatcgagca gcatgtatgg gacatggtga
     9241 tcgactggct ggcggccggc gtggacccgg cacaggcgac actcttcatc cagtcgcgcg
     9301 tgccggagca cgccgagctg cacctgctgc tgtcgatggt gacgccgctt ggatggctcg
     9361 agcgggttcc aacctacaag gaccagcagg aaaagctcgc cgacaaggat ctcgccacct
     9421 acgggttcct gggctatccc ctgctgcaga gcgctgacat cctcatctat cgcgccaacc
     9481 aggtcccggt gggagaggac caggtgcctc acgtcgagtt cacacgcgag gtcgcgcgac
     9541 gtttcaacca cgtgttcggg cgggaggaag gcttcgagca gaaggcggag gccgcgatca
     9601 agaagctcgg cgccaagcgc gcccgcctct atcgccagct gcgcgccaag ttcctcgaga
     9661 agggcgatgc cgaagcgctc gcagcggcgc gcgccctggt agaagaggcg cagaaccttc
     9721 ccatgggcga ccgcgagcgg ctgttcggct atatcgaggg cggcggcaag gtggtgctga
     9781 tggagccgca agcgctgctc acgggagctt cgaagatgcc ggggctcgac gggcagaaga
     9841 tgtccaagtc ctataacaat accatcagcc tgcgcgagga tgccgacagc attgcgaaga
     9901 aaataaggac catgccgacc gacccggcgc gagtcaagcg ttccgacccg ggcgatccgg
     9961 agaaatgccc ggtatggcag ctccacctcg tgtattcgga tgataagcgc aggcagtggg
    10021 tgcaggaggg ctgccgcagc gccggtatcg gctgtctcga atgcaagcag ccggtcatcg
    10081 atgccgtgat cgaggagctg aaaccgatcc gggagcgggc atcgcagtac ctcgaggacc
    10141 cgacgctggt gcgctcgatc attgccgatg gctgcgagaa agcccgcaaa ctggcgcaag
    10201 agacgctgcg cgaggtcagg caggcgatgg gactcgatta cagctgaaaa aagcaggtgc
    10261 cgggagggga atccctgtag aataccccga tgcctccgtc gctattgcgg gacggtggcc
    10321 gcaaggccgc tgaaccgcgt gaactgaccg gcagatcgtc tccgccggca ggcagcttcc
    10381 ccccaaaatt tgcgcaagtc ccggccggcg cacgaccgaa gcggccggaa cgcatctcat
    10441 gaccgaaccc gtcgccgcca atcccatcgc catggtctac gggcagccgg tgctcgagtt
    10501 tccgcacgac ctctacatcc cgcccgatgc gctggaggta ttcctcgatc agttcgaggg
    10561 gccgctcgac ctgctgctct acctcattcg caaggaaaac atcaacgtcc tcgacatccc
    10621 gatggcgcgg ttgaccgccc agtacctcga gtacgtcgaa atgatgcaaa cgcgctcgct
    10681 cgagttagcg gcggagtacc tgctgatggc ggcgatgctg atcgagatca agtcgcggat
    10741 gctgctgccg cggccggccc tgggcgaact ggaagaagac ccgcgcgcag aattggtgcg
    10801 gcgcctgatg gaatacgagc agatgaagct agcagcccac cgcctgaacg agctacccca
    10861 ggccgggcgc gatttttcgc tggtgcaggt gttgttcgag gacgtggccg ccgagagact
    10921 gccggaagta aataccgagg acctgagatc agcctggctt gcgttattga cgcgcgcagc
    10981 ggtaaaccgg catcaccgca tcacgcgcga gcaactgtca gtgcgtgatc acatgacccg
    11041 gatcctgcgg cgcctgcagg aggcgcgcta cgtcgacttc ctggagttgt tcgacccggc
    11101 gcaaggcatc gccgtgcttg tagtgacttt cctcgcgata ctggagctcg cgcgcgaaag
    11161 cctgatcgag gtcagccagc aggcagccta tgctccgata tacgtaaaac tgaaagcagg
    11221 ccaccttgcg gccgtggaat aacgggaaac cctgatgaat aagacctcct ccgacctgga
    11281 acacgtgaag aaggtgctgg aagcggcact gctggtgagc caggagccgc tcccgattgc
    11341 ggaactgaag aaactcttcg acgaggagtt cgccagcgat gttttgcgcc gcgcccttga
    11401 tgaactgcgc aaggactggc agggcagggg ggtcgagctc tcgcaggttg cctccggctg
    11461 gcgcttccgg gcccgtcccg agtatcagcc gtaccttgac cgcctgagcc cgcagaagcc
    11521 gccgcgttac tcgcgcgcgg tactggaaac gcttgccatc attgcttacc ggcagccggt
    11581 cacgcgtggt gacatcgaag agctccgtgg cgtcgtggtt tccgctaaca tcattcgggc
    11641 actggaagcg cgtggctggg tcgaggtggt cgggcacaag gaggtaccgg gccgccccga
    11701 gttgctggcg accaccaagg cgttcctgga cgatctcaat gtgggctcgc tcgaggagct
    11761 tccaccgctg gacgatcttg gctcgctggt ggagcgggcg ccggtgtcgc tgcagccgcc
    11821 ggctgccgat gcaccggagc ccgagatggt cgaagagccg cgtcgggccg cggcttaacg
    11881 ccgcggcggc caggcaggga acgaggggat actcccctcg tacaccccta catcagtcgg
    11941 aaaccagctt gggtcaggaa cgaggggatg ttcccctcgc gcacccctac attagtctga
    12001 aaccggccta ggtctggaac gaggggatgc ttcccccgtc aacggtcagc gggctcgagg
    12061 cgcgccagca cgccattcga ctcgtcggtc agcaggtaaa gaaagccgtc cggcccgttg
    12121 cgcacgtcgc ggatgcggcc cagttcattc ttgagcatcc gttcttcctt taccactttt
    12181 tcaccgtcga gcttcagacg gaccagcatc tggtcgcgca atgcgccgac aaacaggtcg
    12241 ccgcgccagg ccggaaactt gtccccggtg tagaacgcca tcccggaagg cgcaattgaa
    12301 ggcacccagt aatggagggg ttgcgccatg ccgggcttgt gcgtgccttc gccgattttg
    12361 gttccgatga agtactggac gccataggtg atgatcggcc agccatagtt gattcccgcc
    12421 cggatcacgt tgacctcgtc tccgccctgc ggtccgtgct cgtgtgtcca cagcatcccg
    12481 gtttgcggat gcaaagccgc accctgaatg ttgcggttgc cgagcgtgaa cttttccggt
    12541 ttccagcccg gtcttccggc gaagggattg tctatcggcg ttttaccgtc gtcatggagc
    12601 cggatcaccg atcccgcgtg atcggcgggt ttttgcgcgc gttcgtcctc gccgcgatcg
    12661 ccgagcgtga tgtacagata tccctcccgg tcaaatacca gccgtgagcc gaaatggcgg
    12721 ccagtggggc ccttgggcag ctggcggaac aggatctgca cgctctcgag gcgattgccg
    12781 gtcaaacggc cgcgggcgac ttcggtgccg acaccgcctt cgccgcgacc ggcgtaggag
    12841 aagtaaacaa ggttgttctc agcgaagcgg gggtgcagcg cgacgtcgag gagcccgccc
    12901 tggccgtgga ccgccaccgc aggcagcccg gaaacggcct gagatacgag cttgccgtcc
    12961 ttgccgacga tccgcagccg tcccgggcgc tcggtgacca gcatccggcc gtcgggaagg
    13021 aatgcgagcc cccaggggtg ctccagccct tccaccagct tgaccacgcg aacgatgtgc
    13081 tcctgggttt taaacgtttg cgcgctggcg gaatatgaat tcagcgcaag tgctgcgccg
    13141 accaacgtcg cggcgcgcac ggcaggaaag aatttcatag acgcttcaac ggggaacctg
    13201 ccggatctcg aattccaggg tctgcaggtc gccgaacgca taggtcgcgg gcgcgctcac
    13261 gcccgcgact gtcccgggat cgattcgcag cgtttccccg cccttgacgt ttccgatgcg
    13321 ttcgatgacg gcgcggtgct catgtccatt gcataccagg tcatagtctc ccgtgagcgc
    13381 cagtgcccgg gcgtaatgcg ggtagtgcac catgaatatc cgtcttttcg ccaaatccag
    13441 cgatccatcc tggccataat aatgcaaccg gttcctgggt tcctgcgccc actttgacag
    13501 gtaaaacaga tcgccggcgt tgttgccgtg aatcaggtgg atcggcagct ccagtccgat
    13561 gacggcatgc agggtggaag gtgccaccag atcaccgcaa tgcagcacgg cctgagcgcc
    13621 gagcgctttc gcctctgcga cggcagccgc gagcggttcg cggtgatcgt gactgtcgga
    13681 aacaatgcag attttcatgg cggcgattgt aacttataga cgcgcaaagt tttttctatg
    13741 accgcataac cttttctttg caatagggat tccgctatcc cttaaaatca gctcggtcaa
    13801 tcagggggcc tcgatgttca ccagcaatcc atttgccatg ctctcggcgt tcataccgcc
    13861 cgccttcatg ccgaccttcg tggtcatcat ggtcgtcctg gtcgtggccg ggacgctgtt
    13921 cgacatcgtg cacaagggca gtgccaggta tttctttgcc aactggaagg aatcgcggaa
    13981 caaggggaag cagctcggcg gtggcgagct ggcctctatc gccgtccaga ccgcggtcgt
    14041 ggatgtgctg acgtccgggg agttctgcaa cccgcgtcgc cggatcgccc atctgcttac
    14101 catgtacggg ttcctgatct acgccatcac caccgccatc atgatattcg gatatccgac
    14161 gccggcgacg ccgacccccg ccatcctgcc aatgctctgg tggatcggcg gcgtcatggt
    14221 ctgcgtgggc ggctactggt tctggttctt catccgcgtc gacgtggccg ccgagggcaa
    14281 ctcgcctttc cgcgtcatgc acgcggacct cttcattctt tcgctgctcg ccagcgtgac
    14341 gctgggcctg atctgggctt tcctgcagac gaggggcggc gcgggcgcga gcgtgttctt
    14401 cggcctgtac atcatcgcca gcatggtgct gttcggatcg gtgccgtggt ccaagttcgc
    14461 ccacatgttc ttcaagcctg cggcggcctt cgagaaaagg ttgtccaagg ccaacggcac
    14521 ggccgagaat ttgccgactt tgtcccggga cgatcacgaa caacagaaaa gacactccat
    14581 ggaactgttg cgggatgctc cgatggacat ggggcttgga ataaaacgcg aagcgccccg
    14641 ccattactag aaaatacccc gcgagttcca ccgggacata tcaaggagcc gatttatgcc
    14701 taccttcgtc tacatgacaa ggtgcgacgg atgcggacac tgcgtcgaca tctgcccgtc
    14761 cgacatcatg cacatcgaca agacgtaccg gcgcgcctac aacatcgagc ccaacatgtg
    14821 ctgggagtgc tactcctgcg tgaaggcctg tccgcagaac gcgatcgacg tgcgcgggta
    14881 tgccgacttt gccccgctcg gccacagcgt gcgagtccag cgcgacgagc agaaaggcac
    14941 gatcgcctgg aggatcaagt ttcgcaacgg cacggaaaaa aacttcctgt cgcccatcac
    15001 caccaagccc tggggaacgg cgatccccaa gctcgcggat gttcccgcgc ccagccagga
    15061 aatgcgcgac agccagctgt tgtacagcga accgaaattc atccgctttg atgacggcga
    15121 attgcatacg ctgaaggcga gcggcctcaa gatgaagaaa ggcgtgtact actgatggct
    15181 tacaaaacga tcgtcgaaga gggcatcgat atcctggtcg tcggtgcggg cctaggcggt
    15241 agtggcgccg cgtgggaggc ccggttctgg gggcaggaca agaagatcgt cattgccgag
    15301 aaggccaata tcgatcgctc cggcgcggtc gcccagggcc tgtacgccat caactgctac
    15361 atgggtaccc gctggggcga aaataagccg gaggaccatg tccgctacgc ccgtatcgat
    15421 ctgatggggt tggtgcggga ggacctgctg ttcgacatgg cccgccacgt cga
//
LOCUS       DBNGSG010000456         6173 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2015594, whole genome shotgun sequence.
ACCESSION   DBNGSG010000456 DBNGSG010000000
VERSION     DBNGSG010000456.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6173)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2015594"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1501
                     /locus_tag="AC158Q_11915"
     CDS             <1..1501
                     /locus_tag="AC158Q_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HIR3340523.1"
                     /translation="RYAEFHQRAQALVTALRKLGVKRGERVATLMWNHHVHLESYFAI
                     PCMGAVLHTLNLRLSPDDIAYIANHAGDRVLIVDDVLLPLYEKIRDRTKFEKVIVVPL
                     SGAAVSEGYEDYEKLVERGRAVKLPALKEDAPAGMCYTSGTTGRPKGVVYSHRALVLH
                     SLACALPDVMGMSNHDTVTPVVPMFHVNAWGIPFTAVMVGAKLVFPGPHLDAASLLDL
                     YQSEQVTLTAGVPTIWAGILQALDQEPSRWKLPAGMRMVVGGAAAPESMIRAFDRHKL
                     KVIHGWGMTETAPAAAVNYQKSEVTGLGADEQYALRAKQGVALPFVEVRAMAGQGEAP
                     WDGRTMGELEVRGPWIAASYHGLESESDKWSEDGWFRTGDVVTIDARGYIKITDRIKD
                     LVKSGGEWISSVDLENALAGHPKVAEAAVIAVPDAKWGERPLAVVVLKPGQQATQDEL
                     REFLAPGFAKFWLPDTFVFVNEIPRSSTGKMMKAKLRERFSGGAVEEKK"
     gene            1539..2333
                     /locus_tag="AC158Q_11920"
     CDS             1539..2333
                     /locus_tag="AC158Q_11920"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3340524.1"
                     /translation="MDLGIKGRLALVTGSTQGIGRATAETLVAEGVRVIVNGRRQEAV
                     RETVAALSKQGEAHGIAADLVTAEGAHAVAEGAARIGPVDILVNNVGFFEVKPFADIS
                     DQDWLEMFELNVMSGLRLARALFPGMLSRNWGRVVFIASEQSAKPNPDMLHYAMSKTA
                     QVAVARGLAELTKGTRVTVNSVLVAPTWSEGVEVFLEKMAPAAGKTAGEMRKAYFEGP
                     GASSLLQRWAEPAEIAAQVAFLCSERASAVNGAAQRVDGGIVRSLF"
     gene            2421..3014
                     /locus_tag="AC158Q_11925"
     CDS             2421..3014
                     /locus_tag="AC158Q_11925"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HIR3340525.1"
                     /translation="MGKRKLEKALSLYRERHPDGPEPAVRWLPFQLNPDLPGSGITRE
                     EYVARKFGARGKGVNERVVMAGRQVGIPFDYEKMKVQPNTLNSHRLMLYAERGGRQDE
                     LAEELFRAHFTEGANLTDAETLADVAGRAGLDRKAVAAYLASDEGNDLGARAEEEARS
                     AGIGGVPFFIFNRRIGVSGAQDPDTLVQAMEQAVAES"
     gene            complement(3021..3398)
                     /locus_tag="AC158Q_11930"
     CDS             complement(3021..3398)
                     /locus_tag="AC158Q_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ family domain-containing protein"
                     /protein_id="HIR3340526.1"
                     /translation="MTALDAIVERRISEAQARGEFDDLPGAGVPLELEDDLLVPEDLR
                     VAYRILKNSGFLPPELEAHREIRALEQLLSIVEDAAGRTRLLSRINFLLERSPAGRRH
                     GNLRVQRDYLEKLAERFEARRNV"
     gene            3568..4440
                     /locus_tag="AC158Q_11935"
     CDS             3568..4440
                     /locus_tag="AC158Q_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tll0287-like domain-containing protein"
                     /protein_id="HIR3340527.1"
                     /translation="MGLRLKFNLVLLLVFALGLGVTGYISYDLLHKNAREEVLRNAGV
                     MMEAALSMRGYTVGQVGPLLPYSDEKFHPQAVPAYAATEIMSSLRKKYSDYSYKEAAL
                     NPTNPRNRAVEWETDIVNAFRNDPDRAEISGMRETPTGRSLYLARPFQIRNKACLACH
                     TTAAEAPPAMVKIYGPNNGFGWKHMEVIGAQIVSVPMELPIQNANRAFYTFMVSLSAV
                     FVALFVILNLMMSLLIVRPITKMSEAANEISTGNLDIPEFSEKGGDEVSLLAKSFNRM
                     RRSLEKAIELIDKK"
     gene            4535..5962
                     /locus_tag="AC158Q_11940"
     CDS             4535..5962
                     /locus_tag="AC158Q_11940"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR3340528.1"
                     /translation="MADTANALPRGYALHEYRVEALLGAGGFGLTYLATDANLNLKVA
                     LKEYLPGDFAARAGDSTVQPKPGEAVESFQWGLQRFMDEAKTLASFRHPNIVRVMRFF
                     EANKTGYMVMEFVEGKPLPDWIGPRRPLPQQSLQALAGPLLDGLEVIHKAGFLHRDIK
                     PANIFMRDDGSPVLLDFGSARELKGGNQELTAVVSPGYAPLEQYHAQGKQGPWSDLYA
                     LGGVLYWMLTGNKPVEAAARVRLDAMPTAVAAAGGRGYAPGFLAAVDWALKPNEDERP
                     QSVAEFKRRLMGGAQSADADRTVPLTGTEPTVIANPAAPGGMTGLTGIVLDRDQVKRI
                     ETEMARHIGPIATVVVRNAAKKALSIPALAEAVAGEIADEKARASFVRKFVSGEAASR
                     PTGEPTRKSPDVSISQKFTPEVLRRAEASLAQHIGAIARIVVRQAAAKARDEAELYLL
                     IADEIKDPAERKNFVRKAVSISGKR"
     gene            complement(5959..>6173)
                     /locus_tag="AC158Q_11945"
     CDS             complement(5959..>6173)
                     /locus_tag="AC158Q_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3340529.1"
                     /translation="VDLWQFWDTITCPTLLLRGAQSDLLLKETAAAMTRRGPRPRLVE
                     FDGIGHAPMLMAGDQIEVVREFLLGG"
BASE COUNT         1161 a         1864 c         2109 g         1039 t
ORIGIN      
        1 ccgctacgct gaattccacc agcgtgcgca ggcgctcgtg acggcgctga gaaagctcgg
       61 ggtcaagcgc ggcgagcgcg tcgcgacgct gatgtggaac caccatgtgc acctggagtc
      121 ctatttcgca attccctgca tgggcgcggt gctgcatacg ctcaacctgc gcttgagtcc
      181 cgatgatatc gcgtacatcg cgaaccacgc cggcgatcgc gtcctgatcg tggacgacgt
      241 gctgctcccg ctgtatgaaa agatccggga cagaacgaaa ttcgagaagg tcatcgtcgt
      301 gccgctgtcc ggcgctgcgg tttccgaggg ttatgaggat tacgagaagc tggtcgagcg
      361 cggccgggcg gtgaaattac ccgccctcaa ggaggacgcc ccggccggca tgtgctacac
      421 ctcgggcact accgggcggc cgaaaggggt cgtctatagc caccgcgcgc tggtgctgca
      481 ctcgcttgca tgcgcgctgc ccgacgtgat gggcatgagc aatcacgaca cggtgacgcc
      541 ggtagtgccc atgtttcacg tcaatgcgtg gggcatcccg ttcaccgcgg tcatggtggg
      601 ggcgaaactg gttttccccg gcccccatct cgacgcggcc agcctgctcg acctttatca
      661 atccgagcag gtcacgttga ctgccggcgt gcccaccatc tgggcgggca tcctgcaagc
      721 gctcgatcag gaaccgtcgc gctggaagtt gcccgccggc atgcgcatgg tggtaggcgg
      781 cgccgcagcg ccggaatcga tgatccgtgc ctttgatcgc cacaaactga aagtcattca
      841 cggctggggc atgaccgaaa ccgctccggc ggccgcggtg aattaccaga aaagcgaggt
      901 cacgggcctg ggcgccgacg agcagtacgc gctgcgggcc aagcagggcg tagcgctgcc
      961 cttcgtggaa gtgcgcgcga tggcggggca gggcgaagcg ccatgggacg ggcgaaccat
     1021 gggcgagctg gaagtgcgcg ggccgtggat tgcggcgagc tatcacggtc tcgagtcgga
     1081 atcggacaag tggagcgagg atggctggtt tcgcaccggc gacgtcgtca cgatcgacgc
     1141 gcgcggctac atcaagatca ccgaccgcat caaggacctg gtcaagtcag gcggcgaatg
     1201 gatcagctcg gtggacctgg agaacgcgct ggccgggcac cccaaggtgg cggaggccgc
     1261 ggtgatcgcg gtgcccgatg cgaaatgggg cgagcggccg ctggcggtgg tcgtattgaa
     1321 gcccgggcaa caggccacgc aggacgagtt gcgtgaattc ctcgcgcccg ggttcgccaa
     1381 gttctggctg ccggatacct tcgtattcgt aaacgagatt ccccgctcct cgaccggcaa
     1441 gatgatgaag gcgaaactgc gggaacgatt cagtggcggt gccgtggaag agaagaagtg
     1501 acgacgtgac gcagtgatgc agtgacggga gaatcaggat ggacctggga atcaagggca
     1561 ggctggcact cgtgacgggc tcgactcagg gcatcgggcg cgccaccgcg gagacgctcg
     1621 tggccgaagg cgtccgcgtg atcgtcaacg ggcgcaggca agaggcggtc agggaaacgg
     1681 tagcggcgct gtcgaagcag ggggaggcgc atgggatcgc ggcggacctg gtgacggcgg
     1741 agggcgcgca cgcagtcgcg gaaggcgcgg caaggattgg cccggtggac atcctggtca
     1801 acaatgtggg gtttttcgag gtcaagcctt ttgccgatat ttcggaccag gactggctgg
     1861 aaatgttcga gttgaacgtg atgagcggct tgcgccttgc gcgcgcgctg ttcccgggca
     1921 tgctctcccg gaactgggga cgtgtggtgt tcatcgccag cgagcagagc gccaagccca
     1981 atcccgacat gctgcactac gccatgtcga aaaccgccca ggttgcggtc gcgcgcggcc
     2041 ttgccgaact gacgaaaggc acccgcgtca ccgtgaacag cgtgctggtt gcgcccacct
     2101 ggtcggaagg ggtggaggtt ttcctcgaga aaatggcgcc cgcggccggc aagacggccg
     2161 gggagatgcg caaagcctat ttcgaaggcc ctggcgcgtc ctcgctgctt cagcgctggg
     2221 ccgagcccgc ggaaatcgcg gcgcaggtgg cgttcctgtg ctcggagcgg gcatcggccg
     2281 tcaatggcgc ggcgcagcgc gtggatggcg gaatcgtgag atcattgttc taacgagtga
     2341 cgagtgatgg atgatgcgtg atgaatgaga aaacactgca aatagacatc gtgtccgacg
     2401 tggtttgacc ctggtgcttc atcgggaagc gcaagctgga gaaggcgctg agcctttacc
     2461 gcgagcgtca tcccgatggg ccagagccgg ccgtgcgctg gcttccattc cagttgaacc
     2521 cggacctgcc cgggagcggc atcactcgcg aggaatacgt ggcgcgcaag ttcggcgcgc
     2581 gcggcaaggg cgtcaacgaa cgcgtcgtca tggcgggccg ccaggtcggc atcccgttcg
     2641 attacgaaaa gatgaaagtc cagcccaata cgctcaattc acaccggttg atgctgtacg
     2701 cggaacgcgg gggcaggcag gacgaactgg ccgaggagtt gttccgcgcc cattttaccg
     2761 agggcgccaa cctgaccgac gcggagacgc tcgccgacgt cgcggggcgc gcgggactcg
     2821 accgcaaggc ggtggcggcc tatctcgcga gcgacgaggg caacgacctg ggagcccggg
     2881 ccgaggagga agcgcgcagc gcaggcatcg gcggagtccc attcttcatt ttcaaccgca
     2941 ggatcggtgt gtccggcgcc caggatcccg atacgctggt gcaggccatg gaacaggcgg
     3001 tggccgaaag ctagcccgga tcacacgttg cggcgggctt cgaaccgttc cgcgagtttc
     3061 tccaggtagt cccgctgcac ccggaggttt ccatgccgcc tgccggccgg gctgcgttcg
     3121 agcagaaaat tgatacgcga cagcaaccgg gtgcgcccgg cagcgtcttc cacgatcgac
     3181 agcagctgct cgagcgcgcg gatttcgcgg tgcgcttcca gttccggcgg caggaatccc
     3241 gagtttttca ggatgcgata ggcgacacgc agatcctccg gcaccagcaa gtcatcctcg
     3301 agttcgagcg gcacgcccgc gcccggcaaa tcgtcgaact ctccgcgtgc ctgcgcctcg
     3361 cttatgcgtc gttcgactat tgcatccagg gccgtcatga ttgcgtatag tgcggcgggc
     3421 cgggcgcgtt atacgcgtga ttccaaaagc gtgcgctagc gcgatgattc acatagcttt
     3481 atggctgcgc gatcattata ctgaatcacg ccggaacgga cagcgggaaa cgctggggcg
     3541 agaaccattt cacggaaggg gatgacaatg ggattgcggc tcaaattcaa cctggtgctg
     3601 ctattggtgt tcgcgctcgg tctcggagtc accggttaca tttcatacga cctgctgcac
     3661 aagaacgccc gggaggaagt gctgcgcaac gccggcgtga tgatggaggc agcgctctcg
     3721 atgcgcggtt acacggtcgg gcaggtgggg ccgctcctgc cgtacagcga cgagaaattc
     3781 catccgcagg ccgtgccggc gtacgcggcg accgagatca tgagttcgct gcgcaagaaa
     3841 tactccgatt acagctacaa ggaggcggcg ctcaacccca ccaaccctcg caacagggca
     3901 gtggaatggg agaccgacat cgtcaacgcc tttcgcaatg atcccgatcg cgccgagatc
     3961 agcggcatgc gcgagacgcc gaccgggcgc tcgctctacc tggcgcggcc gttccagatc
     4021 aggaacaagg cctgccttgc ctgtcatacc accgccgccg aggcgccgcc cgcgatggtc
     4081 aagatctacg ggccgaacaa cggcttcggc tggaagcaca tggaggtgat cggcgcgcag
     4141 atcgtctcgg tgccgatgga gctgccgatc cagaatgcca accgggcgtt ctacactttc
     4201 atggtatcgc tctcggcggt gttcgtcgcg ctattcgtca tcctcaacct gatgatgagc
     4261 ctgctgatcg tgcgcccgat cacgaagatg tcggaggcgg ccaacgagat atccaccggc
     4321 aacctggaca tccccgagtt ttccgagaag ggcggggatg aagtctcgct gctggcgaag
     4381 tccttcaacc gcatgcgccg cagcctcgag aaggcgatcg aattgatcga caagaagtga
     4441 cgggtggcga gtgacgagtg acgggtttca agattacctt tttcgtaccg gcattcagcg
     4501 gcgttcatcg gcggcgatat cggggatttg agccatggct gacaccgcca acgcgcttcc
     4561 ccgcggctac gcattgcacg agtatcgcgt cgaagcgctg ctcggggcag gaggcttcgg
     4621 gctgacctac ctcgcgaccg acgccaatct caatctcaag gtcgcgctca aggaatatct
     4681 gccgggcgac tttgccgcgc gcgccgggga cagcaccgtg cagcccaagc cgggtgaggc
     4741 ggtcgagtcc ttccagtggg ggctgcagcg cttcatggac gaggcgaaga cgctcgcctc
     4801 gtttcgccat cccaacatcg tgcgggtgat gcgttttttc gaggcgaaca agaccggcta
     4861 catggtcatg gaattcgtcg agggcaagcc gctcccggac tggatcgggc cgcggcggcc
     4921 gctgccgcag cagtcgctgc aggccctggc cgggccgctg ctcgacgggc tggaggtgat
     4981 ccacaaggcg ggcttcctcc atcgcgatat caagcccgcc aatatcttca tgcgcgacga
     5041 cggcagcccg gtgctgctcg atttcggctc cgcgcgggag ctgaagggcg gcaaccagga
     5101 attgaccgcc gtcgtctctc ccggctatgc accgctggag cagtaccacg cccagggcaa
     5161 gcagggcccg tggagcgatc tctatgcgct cggcggtgtc ctgtactgga tgctgaccgg
     5221 caacaagccg gtcgaggcgg cggcgcgcgt gcggttggac gccatgccga ctgcagtggc
     5281 agcggccggc ggcaggggct acgcgcccgg tttcctcgca gccgtggatt gggcgctaaa
     5341 gcccaacgag gacgaacggc cacagagcgt ggccgagttc aagcgcaggt tgatgggcgg
     5401 cgcgcagtcc gcggacgcgg atcgcaccgt gccgctgacc gggacggagc cgacggtgat
     5461 cgccaatccc gcggcgcccg gcggcatgac cggattgacc gggattgtgc tcgaccgcga
     5521 tcaggtgaag cgtatcgaga ccgagatggc gcgccatatc ggcccgatcg caacggtggt
     5581 tgtgcgcaat gccgccaaga aagcgctgtc gatccctgcc ctggcggagg ctgtcgcggg
     5641 cgaaattgcc gacgagaagg cgcgtgcatc ttttgtcagg aaattcgtgt cgggcgaggc
     5701 cgcgagccgg ccgaccgggg agccgacccg gaagtcgccc gatgtctcga tttcccagaa
     5761 attcacccct gaagtcctgc ggagggcgga agcgtctctc gcgcaacata tcggggccat
     5821 tgccaggatc gtagtgagac aggcggcggc aaaggcgcgc gacgaagccg agctctacct
     5881 gctcattgcc gacgagatca aggaccccgc cgagcgcaag aactttgtcc gcaaggcggt
     5941 ttcgatctcc ggcaagcgct agccaccgag caggaattcc cggacgacct cgatctggtc
     6001 gccggccatc agcattggcg cgtgaccgat gccgtcaaat tccaccagcc tgggccgcgg
     6061 cccgcgccgg gtcatggcgg ccgcggtttc cttcaacagt agatcggatt gcgcgccgcg
     6121 cagcagcagc gtggggcagg taatggtgtc ccagaactgc cacaggtcca cgt
//
LOCUS       DBNGSG010000457         5469 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_827021, whole genome shotgun sequence.
ACCESSION   DBNGSG010000457 DBNGSG010000000
VERSION     DBNGSG010000457.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5469)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5469)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5469
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_827021"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..369
                     /locus_tag="AC158Q_11950"
     CDS             175..369
                     /locus_tag="AC158Q_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340530.1"
                     /translation="MADKRDPDKLKGPGGMSMRELHDLVKKGYARDRAAGKPHNKLAQ
                     PQNRWQRLVRYVWDKNRGRG"
     gene            complement(492..1964)
                     /locus_tag="AC158Q_11955"
     CDS             complement(492..1964)
                     /locus_tag="AC158Q_11955"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013079.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family metallopeptidase"
                     /protein_id="HIR3340531.1"
                     /translation="MLARLEQNAAPLSAADLKRARHVLIVLPKMSLPGGLRGIPGAAT
                     LTAALSRRKKKIADLAKAPITADLEQGVLASWVMSDLAQPVFEQQTLLRKGLQPLLAE
                     KPETIAIGVFGPAARRRHAAELAVYSAWVNGARLPDRKKKAEVAAVQTISLHGYRAPD
                     GFSRQFAVAQGNLLCRELTVLPPNELNPGNYRKRVRELARKRGWRHEEYDMKRLRAIG
                     AGAFVAVAQGSAEGDAAIVHLTYRHPRAGKSFAVVGKGICYDTGGHNLKSARSMQGMH
                     KDMNGSAVALGILLAASAMKLPVRIDCWLAIARNDIGPRAYHQDEIVTALNGTTIEVV
                     HTDAEGRMVLADTLALAARSKPDLILDFATLTGSMHVALGERYSGFFATAPELARRAV
                     AAGAVSGERMCAFPLDADYDEALQSEVADVKQCTMDGGADHILAALLLKRFTDGRPWM
                     HVDLSSARCKGGLGAVASDVNGFGVAWGIEFLKMLLARTR"
     gene            2048..2830
                     /locus_tag="AC158Q_11960"
     CDS             2048..2830
                     /locus_tag="AC158Q_11960"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023568.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3340532.1"
                     /translation="MPRLLIFLACLCSCAAVAEDIVTLSTRGGVTQSYLLSAPEAGKA
                     RAVAILFPGGAGKTDLERETARQVLDRGNFLVRSRRLLAANGIAAVVADAPSDRASGM
                     DDEFRLGDAHAEDIGKVVADLKKRFAGVPVFLVGTSRGTVSAASIGKRLGPAVDGVVL
                     TATLFLASGKQPGLSGFDYASIPAPLLFVHHANDGCAYTPYASAKRLSERFPLVTVSG
                     GLPPQSKPCEAMSEHGFLGSEAATVEAIARWMLKQPYSREIN"
     gene            2871..3665
                     /locus_tag="AC158Q_11965"
     CDS             2871..3665
                     /locus_tag="AC158Q_11965"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HIR3340533.1"
                     /translation="MRQYLDLMKHVLEHGARKSDRTGTGTLSIFGAQLRFDLDAGFPL
                     LTTKKVHLKSIIHELLWFLKGDTNTRYLKENGVTIWDEWADAKGDLGPVYGYQWRSWP
                     SPDGRHIDQVSQVLEQIRKNPDSRRMIVSAWNVADLPKMALLPCHAFFQFYVANARLS
                     CQIYQRSADLFLGVPFNIASYALLTLMVAQACGLKPGDLVHTFGDTHLYSNHLEQARE
                     QLSRQPRRLPVMKLNQEVKDLFQFKYEDFSLENYDPHPAIKAPVAV"
     gene            3761..4234
                     /gene="folA"
                     /locus_tag="AC158Q_11970"
     CDS             3761..4234
                     /gene="folA"
                     /locus_tag="AC158Q_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HIR3340534.1"
                     /translation="MAMSRNRVIGAGNAIPWHLPEELKRFKRITLGHHIIMGRKTWDS
                     IGRPLPGRTTVIVTRQHGYGVPGAKIAHSLDEAIAACGDDSEIFVIGGAELYAQALPR
                     AGRLYLTTVDAEVDGDTSMPDFDVGDWCEVSSESFAADGNHRHSFRCTVYERNRP"
     gene            4300..4917
                     /locus_tag="AC158Q_11975"
     CDS             4300..4917
                     /locus_tag="AC158Q_11975"
                     /inference="COORDINATES: protein motif:HMM:NF019843.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3340535.1"
                     /translation="MAGAQDKVDVDDLKVRSAAGDRRATRQLAEMYYVGRGGVEQNFG
                     EAAVWYEKLAKQGDARAQTSLGLMYARGYGVAKNMDTARKWWSFAAAQNDPGAQYNLG
                     VIYSSGEGVAQDYPQAAQWLNRASLRGHVQAQHNLGLLYYEGKGVERDLVRAYYWLKV
                     AALQGDEVSQDALMLVRKGMSADQVQQAEIQAEEWMKKYKKVVGK"
     gene            complement(4936..>5469)
                     /locus_tag="AC158Q_11980"
     CDS             complement(4936..>5469)
                     /locus_tag="AC158Q_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340536.1"
                     /translation="IALQCPDHEHYHVQSENLIVEILDDRGKACAPGEIGRIVFTHLH
                     NFAMPLFRYAIGDHAEAGAPCACGRGLPVIKRILGRQRNILTLPDGRRRWPSFPGGKW
                     MHLAPIRQLQMIQRSRTRIDVRVAADRELTQEEKQRLIAALQDCLGHPFEMQVEQFPD
                     IPRSASHKFEDFISEIV"
BASE COUNT         1013 a         1780 c         1723 g          953 t
ORIGIN      
        1 gcaaccgtcc accacgcacc caatcgcggg cccgtgactt tccccgaagg aggtcacaca
       61 gaacagtttt cccaaggtat tgcccgacat cgccccgtca ccgcctggtc gctaaaactc
      121 gattaaattc aacgacgagt ttaacatagg ggcactcaaa caaggcgcgc tgcgatggct
      181 gacaaacgcg atcctgacaa gttgaaaggc cccgggggca tgtcaatgcg cgagctgcac
      241 gatctcgtga agaaggggta tgcgcgcgac cgtgcggcgg gcaagccgca caacaagctc
      301 gcgcagccgc agaatcgctg gcagcgcctg gtgcgttatg tgtgggacaa gaaccgcggg
      361 cgcggctgag cacggtcagg gtcaggcggt gtcctgtttc tttccgtccg gcggcgcgaa
      421 atatccggtc gcaaaccgcc cggtcccgac cccaatgaaa ttgttgacgc gtgcccagct
      481 cttcaggggc atcaacgcgt ccgggcaagc agcattttca gaaactcgat accccaggcg
      541 accccgaaac cgttgacgtc gctcgcgacc gcgccgagcc cgcccttgca cctggcggac
      601 gacaggtcca cgtgcatcca cgggcggccg tcggtgaagc gtttgaggag cagcgccgcg
      661 agtatgtggt cggccccgcc gtccatcgtg cattgcttca cgtcggcaac ttcactctgc
      721 agcgcctcgt cgtagtccgc gtccagggga aacgcgcaca tccgctcgcc cgataccgcg
      781 cccgccgcca ccgcgcggcg cgcaagctcc ggagcggtcg cgaaaaagcc gctgtagcgc
      841 tcgcccagcg cgacgtgcat gctgccggtc agcgtcgcga aatcgaggat gagatcgggc
      901 ttgctgcgcg cagcaagcgc cagcgtatcg gccagcacca tgcgtccttc ggcgtcggtg
      961 tggacgacct cgatggtggt gccgttcagc gccgtgacga tttcgtcctg gtgataggcg
     1021 cgcggcccga tgtcattgcg cgcgatcgcc agccagcagt cgatccgcac cggcagcttc
     1081 atcgccgagg cagccagcag gatacccagc gccaccgcgg aaccgttcat gtctttgtgc
     1141 atgccctgca tgctgcgggc actttttaaa ttgtggccgc cggtgtcgta gcagatgccc
     1201 ttgccgacga cggcgaacga cttgcccgcc cgcgggtggc ggtaggtcag gtgcacgatc
     1261 gccgcatcgc cctcagcgct gccctgcgcc acggcgacga acgcccccgc cccaattgcg
     1321 cgcagccgct tcatgtcgta ctcctcgtgc cgccagccgc gcttccgggc gagttcgcgc
     1381 acgcgctttc gataattccc cgggttgagc tcgttgggcg gcagtacggt cagctcgcgg
     1441 cacaacaagt tgccttgcgc gacggcgaac tgtcgcgaaa aaccgtccgg cgcgcgataa
     1501 ccgtgcagtg atatcgtctg cacggcagct acttctgctt ttttcttgcg gtcgggcagc
     1561 ctggcgccgt tcacccaggc cgaatagaca gccagctccg cggcatgccg ccggcgggcg
     1621 gctgggccga aaacaccgat tgcgatggtt tccggctttt cggcaagcag cggctgcaac
     1681 cctttgcgca gaagcgtttg ctgctcgaat accggctgcg ccaggtcgga catcacccac
     1741 gacgccagca cgccctgctc gagatcagcg gtgatcggcg ccttcgccag atcggcgatc
     1801 ttctttttgc gccgcgacaa ggccgccgtg agcgttgccg cgccggggat gccgcgcagg
     1861 ccgccgggca aagacatctt cggcaacacg atgagaacgt gccgggcgcg tttcagatcg
     1921 gccgcggata acggtgcggc attttgttcc agcctggcca gcatgaggat ttcctaagcc
     1981 attgatcggg aagagcgcgg attataacgt ccgcctcggg cggcaacccg ggagtacaat
     2041 ccatcgcatg ccacgcctgt tgattttcct tgcctgcctg tgctcctgcg ccgccgtcgc
     2101 agaggacatc gtgacgctgt ccacgcgcgg gggcgtgacc cagtcctatt tgctgtcggc
     2161 cccggaagca ggcaaggcgc gtgccgtggc gatactgttt ccgggcggcg ccggcaagac
     2221 cgacctcgag cgcgaaacgg cccgccaggt cctggaccgc ggcaacttcc tggtgcgctc
     2281 gcgccgtttg ctcgctgcca acgggattgc cgccgtggtg gcggacgcac cgtccgaccg
     2341 ggcgagcggc atggatgatg agttccgcct gggcgacgcg catgccgagg atatcggcaa
     2401 agtcgtcgcg gacctgaaaa aacgctttgc gggtgtcccc gtgtttctcg tgggcaccag
     2461 ccgcggcacc gtttccgctg cgtcaatcgg aaaacggctt ggtccggccg tggacggcgt
     2521 ggtgctgacc gcgacgctat tcctcgccag cgggaaacag ccgggattga gcggcttcga
     2581 ttacgcctcc atcccggctc cgctgctctt tgtgcaccac gccaacgacg ggtgcgccta
     2641 tacgccctac gcgtcggcca agcggctgtc cgaacgcttt ccgctggtga cggtatccgg
     2701 cgggctgccg ccgcagtcca agccttgcga agcgatgagc gagcacggtt tcctcggcag
     2761 tgaagcggcg acggtggaag caatcgcaag atggatgctg aagcagccct actcccggga
     2821 aatcaattag aatttccgtt gatcccggcc tgaatcccga gcccgcagta atgcgccaat
     2881 accttgacct gatgaagcac gtgctcgagc acggcgcgcg caagagcgat cgcaccggca
     2941 ccggcacgct ctcgatcttc ggagcccagc tccgcttcga cctcgatgca gggtttcctc
     3001 tgctcacgac caaaaaggtg cacctgaagt ccatcatcca cgagttgttg tggttcctga
     3061 agggcgacac caatacgcgc tacctgaagg aaaacggcgt caccatctgg gacgagtggg
     3121 cggatgcgaa aggcgacctg ggcccggtct acggttacca gtggcgctcg tggccatcgc
     3181 cggatggccg gcacatcgac caggtgagcc aggtgctcga gcagattcgc aagaaccccg
     3241 attcgcgcag gatgatcgtc tcggcgtgga acgtggccga cctgccgaag atggcgctcc
     3301 tgccgtgcca cgccttcttc cagttctacg tcgcgaatgc aaggctgtcg tgccagatct
     3361 accagcgcag cgccgatctt ttcctcggcg tgccgttcaa catcgcgtcc tacgcgctgc
     3421 tcaccctgat ggtggcgcag gcgtgcgggc tgaagccagg cgacctcgtg cacaccttcg
     3481 gcgacaccca tctttattcg aaccacctcg agcaggcgcg cgagcagctc tcgcgccagc
     3541 cgcgccggct gccggtgatg aagctcaatc aggaggtgaa agacctgttc cagttcaagt
     3601 acgaagactt cagcctcgag aactacgacc cgcacccggc catcaaggcc cccgtggccg
     3661 tctgagcgac gggtgacgag tgacggctgt cgggaaaaat ccgggcgcgg caccagccga
     3721 ttcgctaatc acgaatcacg attcacgcct ttcaatcatc gtggcgatgt ccaggaaccg
     3781 cgtgatcgga gcaggcaatg ccatcccctg gcacctgccc gaggaactga agcgcttcaa
     3841 acgcattaca ttgggccacc acatcatcat gggccgaaag acctgggact cgatcgggcg
     3901 gccgttgccg gggcgcacca cggtgatcgt cacgcgccag cacggctacg gcgtgccggg
     3961 cgcgaagatc gcccactcgc tcgacgaagc catcgccgcc tgcggcgatg acagcgaaat
     4021 cttcgtgatc ggcggagccg agctctacgc gcaggcgctg ccccgcgccg gccgcctgta
     4081 cctcaccacg gtcgacgccg aagtcgacgg tgatacgtcc atgccggatt tcgacgtcgg
     4141 cgactggtgc gaggtatcat cggaatcgtt tgccgccgac gggaatcacc gccattcctt
     4201 tcgctgcacg gtctacgaac gcaaccgccc atgagcgccg gtaactcaaa atcacttcgg
     4261 gccgccatcg ctgcctgctt tctcgcagcc tgtttcggca tggccggcgc ccaggacaag
     4321 gtcgatgtcg acgacctcaa ggtccgttcc gccgcgggcg acaggagggc gacccgccaa
     4381 ctcgccgaga tgtactacgt gggccgaggc ggggtcgagc agaattttgg cgaggcggcg
     4441 gtctggtacg aaaagctcgc caagcagggc gatgcgcgcg cgcagaccag cctcgggctg
     4501 atgtacgcgc gcggctacgg ggtcgcgaag aacatggata ccgcgcgcaa atggtggagt
     4561 ttcgccgccg cccagaacga ccccggcgcg caatacaacc tcggcgttat ctactccagc
     4621 ggcgagggcg tcgcgcagga ttacccccag gccgcacaat ggctcaatcg cgcgtccctg
     4681 cgcggccacg tgcaggcgca gcacaacctc gggttgcttt attacgaagg caaaggggtt
     4741 gagcgcgatc tggtgcgtgc ctactactgg ctcaaggtag cggcgctcca gggcgacgag
     4801 gtgtcgcagg atgcgctgat gctcgtgcgc aagggcatga gcgcggacca agtgcagcag
     4861 gctgaaatcc aggccgaaga atggatgaag aagtacaaga aggtcgtcgg caagtgaccg
     4921 cccaggcacg ccgcgtcaca cgatctcgga tatgaaatcc tcgaacttgt gcgatgcgct
     4981 gcgcggaata tcgggaaact gctcgacctg catttcgaag gggtgaccga ggcagtcctg
     5041 caacgcggcg atcagacgct gtttttcctc ctgcgtgagc tcccggtcgg ccgccacgcg
     5101 cacgtctatg cgggtacgtg agcgttgaat catctgcagt tgtcgtatag gcgcaaggtg
     5161 catccatttg cctcccggga aactgggcca gcgccggcgt ccgtcgggca gggtcagaat
     5221 attgcgctgc cgtccgagga tgcgcttgat cacgggaagg ccccggccgc atgcacaagg
     5281 cgcgccggct tcggcatggt ccccgatggc gtagcgaaac aacggcatcg cgaaattgtg
     5341 caggtgggtg aacactatcc tgccaatctc cccgggcgca caagccttgc cccgatcatc
     5401 cagtatctcg acgatgagat tttcggattg aacgtggtaa tgctcatggt ccgggcactg
     5461 cagcgcgat
//
LOCUS       DBNGSG010000458         3696 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_487921, whole genome shotgun sequence.
ACCESSION   DBNGSG010000458 DBNGSG010000000
VERSION     DBNGSG010000458.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_487921"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="AC158Q_11985"
     CDS             complement(<1..544)
                     /locus_tag="AC158Q_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chain length determinant protein tyrosine kinase
                     EpsG"
                     /protein_id="HIR3340537.1"
                     /translation="MDQNAASTRREPTLGTRVASLEDRPIGRILVDMGKLKPRDVDRV
                     FRVHREQGLRFGEAARKLRLVRDADVQYALSVQFSYPYLKPGQGALGPELVTAHEPFD
                     AQSEVLRDLRTQMVLHWVDSERKSLVVASADPRDGRSYLAANLAVVFAQLGGKTLLID
                     GDMRNPRQHRIFGQGNGPGLA"
     gene            complement(550..2025)
                     /locus_tag="AC158Q_11990"
     CDS             complement(550..2025)
                     /locus_tag="AC158Q_11990"
                     /inference="COORDINATES: protein motif:HMM:NF025179.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HIR3340538.1"
                     /translation="MSFTQFLTILRVRAALILVVVLLAGAAAALVTQALPERYLATAS
                     VVIDPNVTQAAGGASSGRPDEFMSTHLDLVANPAVAAGVSDALGLENRPGIGVLLSGS
                     SLMRALLQLTAMLPDSEGQDSAAMSPRDWIADRLLRNLTVRANRDSRIIRITYAAPDP
                     NFAAEVANAFARSYLDTVRQLQVGPAQEYAQSFEEPIKNLKRDLEQAEAKFARFQQQK
                     GIVATDERMDLESARLNDLSSQVVAAQSLSYESEARQRQLRDYLARGGGDGPAEIMSS
                     PVVQQLKQSVSDREAKLAELSKRIGPNHPQHRAAVTELEGLKAQLSEQTRAAAQGLLT
                     SGGVASQREGALRAAMEQQRRRVLALKSDRNTLAMLAREVDGARQAYEAAVQRVTQSR
                     VTSHAGQTSATVVNSATPPMNPASPKPALNLAIGLAAGLVLGIGLALYRESVDGYVRS
                     ERDVIEILGAPVLAVLLPRGGRRDIRALQGPNVLSLPHRPG"
     gene            complement(2051..2971)
                     /gene="epsE"
                     /locus_tag="AC158Q_11995"
     CDS             complement(2051..2971)
                     /gene="epsE"
                     /locus_tag="AC158Q_11995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein EpsE"
                     /protein_id="HIR3340539.1"
                     /translation="MTARAIAIALLLTGTVLISACATGMRQDPAPVQSAAPGKKAAAV
                     EQAAAASGQSPAAAEAQEAAAAGEYRLGPGDVVKISVYNNPDLAMETEITQSGKISFP
                     LIGEVMLGGLTRAGAEKAIADSLAKGGFVPNAHVNLLVAQYRSQQISVMGEVNKPGQY
                     PVSRAATLTEMLAMAGGITAKGSHIITVIQKDGNGRTVQQEVDVKRLFAGGAAANLRL
                     GYDDVIYVPPMPVFYIYGEVRQPGSYPLTPDLTVRQALSLGGGLTVRGTERGIRLERK
                     GKGNETLSRRPSLNDKLQPNDVLHIPESWF"
     gene            complement(2991..>3696)
                     /locus_tag="AC158Q_12000"
     CDS             complement(2991..>3696)
                     /locus_tag="AC158Q_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006712706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HIR3340540.1"
                     /translation="TASIYFVPSTLPFEPIQARVEYIGGIPAIAVCETPFCGINGVLK
                     RTFDLVITGAVLLLIWPLLLAIAAGIKLSSPGPVLFRQRRYGLDGKEILVCKFRTMSV
                     CEDGNRIEQATRNDSRITPFGALLRNTSLDELPQLFNVIGGSMSLVGPRPHAVAHNEQ
                     YRGLIDGYMIRHKVKPGITGWAQVNGWRGETETLDKMKKRVEHDLDYLKHWSLSLDLW
                     ILFRTAWVVLLDRNAY"
BASE COUNT          537 a         1269 c         1239 g          651 t
ORIGIN      
        1 gcgcgagtcc cggcccgttg ccctgcccga agatgcggtg ctggcgcgga ttgcgcatgt
       61 cgccgtcgat cagcagcgtt ttcccgccca gctgcgcgaa taccacggcg aggttcgcgg
      121 ccaggtaact cctgccgtcg cgggggtcgg cgctggcgac gaccagcgac ttgcgctccg
      181 aatccaccca gtgcagcacc atctgcgtcc gcagatcgcg caggacttcg gactgggcgt
      241 cgaatggctc gtgagcggtc accagttccg ggcccagggc accctgcccc ggcttgaggt
      301 acggatagct gaactggacc gagagcgcgt actggacgtc ggcatccctc accagcctca
      361 acttgcgggc cgcctcgcca aaacgcaggc cctgctcccg gtgcacgcga aaaacgcggt
      421 ccacgtcccg cggcttgagc tttcccatgt cgacgaggat gcggccgatc ggccggtcct
      481 ccaggctcgc cacccgcgtt cccagcgtgg gttcgcggcg ggtgctggct gcgttctggt
      541 ccatgattgt catcccgggc ggtggggcag tgaaagaacg ttggggccct gcagggcgcg
      601 gatgtcgcgc ctgccgcccc tgggcagcag caccgccaac accggcgcgc ccagtatctc
      661 gatgacgtcc cgctcgctgc gcacgtatcc gtcgacgctt tcgcggtaga gcgcgagacc
      721 gatgcccagc accagtcccg cggccagccc gatcgccagg ttgagcgccg gcttggggct
      781 ggcgggattc atcggcggcg tggcggagtt gaccaccgtc gcgctggtct gtccggcgtg
      841 actggtcacc cggctctggg tcacgcgctg caccgcggcc tcgtaggcct gccgggcgcc
      901 gtccacctcg cgcgccagca tcgcgagcgt gttgcggtcg ctcttcagcg ccaggactct
      961 tcttcgctgc tgctccatgg cggcgcgcag ggcgccttcg cgctgcgacg cgacgccgcc
     1021 gctggtgagc aggccctgcg ccgccgcccg cgtctgctcg ctcagctggg ccttcaggcc
     1081 ctcgagctcg gtcacggcag cgcggtgctg gggatggttc gggccgatgc gcttggagag
     1141 ctccgccagc ttggcctcgc ggtcggagac gctctgcttc aactgctgca ccacggggct
     1201 cgacatgatt tcagccggtc cgtctccccc gccgcgcgcc agataatcgc gcaactgccg
     1261 ctgtctcgcc tcgctctcgt aggacagcga ttgcgcggcg acgacctggc tcgacaggtc
     1321 gttcaaccgc gcgctctcga ggtccatgcg ctcgtcggtc gccacgatgc ctttctgctg
     1381 ctggaacctg gcgaacttcg cttccgcctg ctcgaggtcg cgcttcaggt tcttgatcgg
     1441 ttcctcgaac gactgggcgt attcctgggc cgggccgacc tggagctggc ggacggtatc
     1501 gaggtaggac cgcgcgaacg cgttggccac ctcggcggcg aaattgggat ccggcgccgc
     1561 atacgtgatc cgtatgatgc ggctgtcgcg gttcgccctg acggtgaggt tgcgcagcag
     1621 gcgatccgcg atccagtcac gcgggctcat cgcggccgag tcctgtccct cgctgtccgg
     1681 cagcatggcg gtcagctgga gcagggcgcg catcagcgac gaccccgaca acagcacgcc
     1741 aatgccgggc ctgttctcga ggccgagcgc gtccgaaacg ccggccgcga cggccgggtt
     1801 ggcgacgagg tcgagatggg tcgacatgaa ttcgtcaggc ctgcccgagg aggcgccccc
     1861 ggccgcttgc gtcacattcg ggtcgatgac cacgctcgcg gtggccaggt agcgctcggg
     1921 cagcgcctgg gtcaccagcg ccgcggcggc cccggccagt aacacgacca cgaggatgag
     1981 cgcggcgcga acgcgtagga tcgtcagaaa ctgcgtgaag ctcatgttcg ttctccgttg
     2041 gcggcgacgc tcagaaccag ctctccggta tgtgcagcac gtcgttcggc tgcaacttgt
     2101 cgttgagact cgggcggcgg ctcagcgtct cgttgccctt gcccttgcgc tccagccgga
     2161 tcccgcgctc ggttccgcgc accgtgagcc cgccgccgag cgacagcgcc tgccgcaccg
     2221 tcaggtccgg cgtcagcgga taggagcccg gctggcgcac ttcgccgtag atgtagaaca
     2281 ccggcatcgg cggcacgtaa atgacgtcgt cgtagccgag gcgcaggttc gcggcggcgc
     2341 cgcccgcaaa cagccgcttg acgtcgactt cctgctgcac cgtgcggccg ttgccgtcct
     2401 tctggatgac cgtgatgatg tgcgagcctt tcgcggtgat gccgcccgcc atggcgagca
     2461 tttcggtcag ggtggccgcc cggctgaccg ggtactggcc cggcttgttc acttcgccca
     2521 tcaccgaaat ctgctggctg cgatactggg ccaccagcag gttgacgtgg gcgttgggaa
     2581 cgaaaccgcc cttggccagg ctatccgcaa tggctttttc ggctccagcc cgggtgaggc
     2641 cgccgagcat cacctcgccg atcagcggga aactgatttt cccgctctgc gtgatctcgg
     2701 tttccatggc gagatcggga ttgttgtata ccgagatctt gaccacgtct cccgggccga
     2761 ggcggtactc gccggctgcg gccgcctcct gggcctccgc ggccgcgggg gattgccccg
     2821 acgccgcggc ggcctgctcg accgccgcgg ccttcttccc gggcgcagcg gattggacgg
     2881 gagccgggtc ctgccgcatc cccgtcgcgc aggcggaaat cagcacggtg ccggtcaata
     2941 acagggcgat ggcgatggcg cgcgcggtca tgtgatcctc cacctcttca tcaataggcg
     3001 ttgcggtcga gcaggaccac ccacgcggtc ctgaacagga tccagagatc gagcgagagc
     3061 gaccagtgct tgaggtaatc gagatcgtgc tcgacccgct tcttcatttt gtccagggtc
     3121 tcggtctcgc cgcgccagcc gttgacctgc gcccacccgg ttatgccggg cttcaccttg
     3181 tggcggatca tgtagccgtc gatgagtccg cggtactgct cgttgtgcgc caccgcgtga
     3241 ggccgcggcc cgaccaggct catcgagccc ccgatcacgt tgaataactg cggcagttcg
     3301 tcgagcgaag tattcctgag caacgcgccg aacggcgtga tccggctgtc gttgcgggtg
     3361 gcctgctcga tccggttgcc gtcctcgcac accgacatcg tgcggaattt gcagaccagg
     3421 atttccttgc cgtcgaggcc atagcgccgc tggcggaaca ggaccggccc gggcgaggag
     3481 agcttgatgc cggcggcgat cgccagcagc agcggccaga tcagcaacag gaccgcgccc
     3541 gtgatgacca gatcgaacgt gcgcttcaac accccgttga ttccgcagaa cggcgtctcg
     3601 cacacggcga tcgccgggat gccgccgatg tattcgacgc gcgcctggat cggctcgaac
     3661 ggcagcgtgc tcggcacgaa atagatcgag gccgtc
//
LOCUS       DBNGSG010000459         4403 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_318380, whole genome shotgun sequence.
ACCESSION   DBNGSG010000459 DBNGSG010000000
VERSION     DBNGSG010000459.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4403)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_318380"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="AC158Q_12005"
     CDS             complement(<1..586)
                     /locus_tag="AC158Q_12005"
                     /inference="COORDINATES: protein motif:HMM:NF024491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HIR3340541.1"
                     /translation="MNGFVEGNRITLLKSGVEYFPALVAEFEAAQREIRLETYIFEAD
                     ATGRAIAAALGRAARRGVAVNVMVDGFGSQDLDPALVRELEEAGVRFLVYRRNISPWT
                     LRRTRLRRLHRKIAVIDARVAFVGGINVVDDVDAQGEAAPRFDYAVRVEGPLLTKIYP
                     VVKQLWSRVSAIQFHARAAEPRALVPPALSRGGQR"
     gene            complement(583..1341)
                     /locus_tag="AC158Q_12010"
     CDS             complement(583..1341)
                     /locus_tag="AC158Q_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR3340542.1"
                     /translation="MRALRVASYNIHKGFSVTPLFNRRLTIHDLRDKLRKLDADIVFL
                     QEVHGQHDRHAHRYHNWPVAPQYEFLADTVWHDFAYGRNAVYDAGHHGNAVLSRFPIQ
                     SWDNQDISSHRFESRGLLHCEIPIPAWNETLHCVCVHLGLRSHERSHQMEKLRQRIER
                     LVPPAAPLIVAGDFNDWRQHATQELAHPLNLYEVFELVRGRPARTFPAALPMMALDRI
                     YVRGFKVPTAHVHHGRSWARVSDHAALTATLVRL"
     gene            complement(1438..2982)
                     /locus_tag="AC158Q_12015"
     CDS             complement(1438..2982)
                     /locus_tag="AC158Q_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340543.1"
                     /translation="MSRASSGTLYQSRLISNALWATLFFLTLVAGCAVAPRQDVAAGP
                     EEECREAFRRVDRAIAEAGVRDGMAARVAGFPYLRVNRFLASYAGGEMSDAQFSEWMD
                     RLTGLGSQSHSIEFANLPADRSEVLGHELWSIDRSRMWVASTLADCAVSLAGADFGDP
                     ARRAALRAVAAVPDDYSAWQRVAGLYWLTRVPFASGVRRWHEEVRATFALPLAALPVS
                     GELRSYAPPPGRLTAVQVAALLKLASANALGIPEPRGADLDALFRTFAPVFTVDTAAD
                     ADLPGELGWGESGVPRVVSRLPVVYRRVSHARYQGRALLQLNYWLWFPERPKGAGSDL
                     LAGHLDGLLWRVTLAPDGAPWVFDSIHPCGCYHLFFPTARAVPKAQPDTLDETAFVPQ
                     ALPRIAPGARVELRIESGTHYLQRIVLRDDPAQGAAEYVFAEDDSLRSLPLPGGGRRS
                     LFRPDGIVQGSERGERWLFWPMGVPEPGAMRQWGRHATAFVGRRHFDDPGLLERYFIL
                     KGVNGE"
     gene            complement(3017..3571)
                     /locus_tag="AC158Q_12020"
     CDS             complement(3017..3571)
                     /locus_tag="AC158Q_12020"
                     /inference="COORDINATES: protein motif:HMM:NF015331.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3340544.1"
                     /translation="MKPIARPLLLALLLVLAPARAAEIIVHVTRNGEALHVDARAEFE
                     GTVAGAWQVLTDYGRLAEFVPDLESSRVISRDGNRVQVEQKGQARLLFFSFPIDVRLD
                     ITEQPKMRIVSRAVAGNFREMVGAYTVEAERGRVYLRYIGRMVPDFYVPPLIGTLALR
                     HNVESTFGALVEEIERRQALPQKP"
     gene            complement(3618..>4403)
                     /gene="nadA"
                     /locus_tag="AC158Q_12025"
     CDS             complement(3618..>4403)
                     /gene="nadA"
                     /locus_tag="AC158Q_12025"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006883.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HIR3340545.1"
                     /translation="ADMASVAKVERAWREIATVLDPDQHVTPVTYINSAADLKAFCGE
                     HGGIVCTSSNARQILDWSLARREKVLFFPDQHLGRWTGYLMGIPLAEMLVWDPDEELG
                     GLTPQQIKMAKILLWKGHCSVHQMFQPQHILRWREKNPSGMVISHPECKFDVCKLSDY
                     VGSTDFIIKTVASSPPGTRWLVGTELNLVNRIAGQFESQGKLVQFMAPTVCMCSTMAR
                     IDPQHFAWALENLVAGKVVNQIKVPRQDAELARIALDRMLAVS"
BASE COUNT          756 a         1519 c         1454 g          674 t
ORIGIN      
        1 cgcgctgtcc cccgcgtgat agcgccggcg gcacgagcgc gcgcggctcc gctgcgcgcg
       61 cgtggaactg gatcgcgctc acccgcgacc acaactgctt caccacggga tagatcttcg
      121 tcagcagcgg cccctcgacg cgcaccgcgt aatcgaagcg cggcgctgcc tcgccctgcg
      181 cgtccacgtc gtccaccacg ttgatgccgc cgacgaaggc aacgcgcgcg tcgatgaccg
      241 cgatcttgcg gtgcaggcgg cgcaggcgcg tgcgccgcag ggtccagggc gaaatattcc
      301 ggcgatagac caggaagcgc acgccggcct cctccagttc ccgcaccagc gcggggtcca
      361 ggtcctgcga gccgaagcca tccaccatga cattgaccgc gacgccgcgg cgcgcggcgc
      421 gcccgagcgc cgcggcgatg gcgcgcccgg tcgcgtcggc ttcgaagatg taggtttcca
      481 ggcggatctc gcgctgcgcg gcctcgaatt cggccaccag ggcgggaaag tattccactc
      541 cgcttttcag cagcgtgatg cggttgccct cgacgaaccc gttcacaatc gcaccaaagt
      601 ggccgtcagg gcggcgtggt cggacacgcg cgcccacgaa cgtccgtggt ggacgtgggc
      661 cgtgggcacc ttgaagccgc gcacgtaaat gcggtcgagc gccatcatcg gcagcgccgc
      721 cggaaaggtg cgcgcgggcc gccccctcac cagctcgaat acctcgtaca ggttcaacgg
      781 gtgcgccagc tcctgcgtgg cgtgctggcg ccagtcgttg aaatcgccgg cgacgatgag
      841 cggcgccgcg ggcggcacca ggcgctcgat gcgctggcgc agcttttcca tctgatggct
      901 gcgctcgtgg ctgcgcaagc cgagatgcac gcacacgcaa tgcagcgtct cgttccaggc
      961 cggaatcggg atctcgcaat ggagcaggcc gcggctttcg aagcggtggc tggaaatgtc
     1021 ctggttgtcc cagctttgga tcgggaaacg cgaaaggacc gcgttgccgt gatggccggc
     1081 gtcgtagacc gcgttgcggc cgtaggcaaa atcgtgccag acggtatcgg ccagaaactc
     1141 gtattgcggc gcaaccggcc agttatgata gcggtgggcg tgacggtcat gctggccgtg
     1201 cacttcctgg agaaacacga tatcagcgtc gagcttgcgc agcttgtcgc gcaggtcgtg
     1261 tatcgtcagc cgccggttga acagcggcgt gaccgagaag cccttgtgga tgttgtagct
     1321 cgctacccgc agcgcgcgca tgacagaaaa tgcgtgattg gtgattggtg attggtgatt
     1381 ggacaagaac ttacaccata acgagctttc cggccggttc agtccattca ctattcacta
     1441 ttcaccattc acgcctttca gaatgaaata gcgctcgagg agcccggggt cgtcgaaatg
     1501 gcgccgcccg acgaaggcgg tggcatgccg tccccactgg cgcatcgcgc cgggttcggg
     1561 cacgcccatc ggccagaaca gccagcgctc gccgcgctcg ctgccctgga cgatgccatc
     1621 gggccggaac aggctgcggc ggccaccgcc gggcagcggc agcgagcgca ggctgtcgtc
     1681 ctccgcgaac acatactccg cggcgccctg cgccgggtca tcccgcagga cgatgcgctg
     1741 caggtagtgg gtccccgatt cgatgcgcag ttcgacacgc gcgcctggcg cgatgcgcgg
     1801 cagcgcctgc ggcacgaaag ccgtctcgtc cagcgtatcg ggctgcgctt tcggcacggc
     1861 ccgcgcggtc ggaaagaaca ggtgatagca gccgcacggg tggatcgagt cgaacaccca
     1921 cggcgcgccg tccggcgcaa gcgtcacgcg ccacagcagg ccatcgagat gccccgccag
     1981 caggtccgag ccggcgccct tcgggcgctc gggaaaccac agccagtagt tgagctgaag
     2041 caatgccctg ccctggtagc gggcatgcga gacccggcgg tacaccaccg gcaggcgcga
     2101 cacgacgcgc gggaccccgc tctcgcccca accgagctct cccggcagat ccgcgtctgc
     2161 cgccgtgtcc accgtaaata ccggcgcgaa cgtcctgaac agggcgtcga ggtccgcgcc
     2221 acgcggctcg gggatgccga gcgcattggc ggaagcgagc ttgagcagcg cggcgacctg
     2281 caccgcggtc aaccggcccg gcggcggcgc atagcttcgc agctcaccgg ataccggcag
     2341 cgcggcgagt ggcaacgcga atgtcgctcg cacctcctcg tgccatcgcc gcacgccgct
     2401 tgcgaacgga acgcgcgtga gccagtacag cccggccacc cgctgccacg ccgagtagtc
     2461 gtccggcacg gccgccaccg ccctcagcgc cgcgcgccgc gccggatcgc cgaaatcggc
     2521 gccggcaagg ctcaccgcgc agtcggccag cgtggacgcg acccacatgc gggagcggtc
     2581 gatgctccac aattcatggc caagcacctc ggacctgtcg gcgggcaggt tggcgaactc
     2641 gatcgaatgc gactggctcc ccaggccggt caggcggtcc atccattcgg agaattgcgc
     2701 atcgctcatc tccccgccgg cgtacgatgc gaggaaccgg ttgacgcgca agtatggaaa
     2761 ccccgcgacg cgcgccgcca tcccgtcccg gacccccgcc tcggcaatcg cccggtccac
     2821 gcgccggaaa gcctcgcggc attcctcttc cggcccggcg gcaacatcct ggcgcggcgc
     2881 gacggcgcag cccgccacca gcgtcaggaa aaacagcgtg gcccacaagg cgttgctgat
     2941 aaggcgtgac tgataaagcg tgcctgatga tgcgcggctc atggacatgc tcgactcacg
     3001 aatcacgatt cacgattcac ggcttctgag gcagcgcctg ccgccgctcg atctcctcca
     3061 ccagggcacc gaaggtggac tcgacgttat gcctgagcgc cagggtgccg atcagcggcg
     3121 ggacgtaaaa atccgggacc atgcgcccga tgtagcgcag atacacccgc ccccgctccg
     3181 cttccactgt gtaggcgccc accatctcgc ggaagttccc tgccaccgcg cgcgagacga
     3241 tccgcatttt gggctgctcg gttatgtcga gccgcacgtc gatgggaaag ctgaaaaaca
     3301 gcagccgcgc ctggcccttc tgctcgacct ggacccggtt gccgtcgcgg gaaatcacgc
     3361 ggctcgactc caggtcgggg acgaattccg ccagccggcc gtagtcggtc agcacctgcc
     3421 aggcgccggc gacggtgccc tcgaattcgg cgcgcgcatc cacatgcagc gcctccccgt
     3481 tgcgggtgac gtgaacgatg atttccgcgg cccgggctgg cgcgagcacc aggagcagcg
     3541 cgagcagaag cgggcgcgcg atgggcttca cggcccatag cctagcaccc gggttcgtcg
     3601 gcggggggcc ggaagcgcta ggagaccgcg agcatgcggt cgagcgcgat gcgcgcgagt
     3661 tcggcgtcct gccgcggcac cttgatctgg ttcactactt tgccggcgac caggttttcc
     3721 agcgcccagg cgaaatgctg gggatcgatg cgcgccatgg tcgagcacat gcacaccgtg
     3781 ggcgccatga actgcaccaa cttgccctgg gactcgaact gcccggcgat gcggttcacg
     3841 aggttcaact cggttcccac cagccagcgc gtgcccggcg ggctcgacgc gaccgtcttg
     3901 atgatgaaat cggtggagcc gacgtagtcg gagagcttgc acacgtcgaa cttgcactcc
     3961 gggtgggaga tcaccatgcc ggacggattc ttctcgcgcc agcgcaggat gtgctgcggc
     4021 tggaacatct ggtgcaccga gcaatgcccc ttccacagca ggatcttcgc catcttgatc
     4081 tgctgcggcg tcaacccgcc cagctcctcg tccgggtccc acaccagcat ctcggccagc
     4141 gggatgccca tgaggtagcc ggtccagcgc cccaggtgct ggtcggggaa gaacagcacc
     4201 ttctcgcgcc gcgcgagcga ccagtcgagt atctggcgcg cattggagga cgtgcagacg
     4261 atgccgccat gctcgccgca gaacgccttc aggtccgccg ccgaattgat gtaggtcacc
     4321 ggggtgacgt gctggtcggg atcgagcacg gtggcgatct cgcgccaggc gcgctcgacc
     4381 ttggcgacgc tcgccatgtc cgc
//
LOCUS       DBNGSG010000460         5148 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4394004, whole genome shotgun sequence.
ACCESSION   DBNGSG010000460 DBNGSG010000000
VERSION     DBNGSG010000460.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5148)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5148)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5148
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4394004"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="AC158Q_12030"
     CDS             <1..519
                     /locus_tag="AC158Q_12030"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020039.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR3340546.1"
                     /translation="LVYLGYGEMGLNPLPCKVNVSIAVWPWGAYLGEEALKHGVRMKI
                     SSWRRHDPNALPPAAKGTGMYLNSSMAKLEAVKAGGTLGKARLFHQYTGPERGVPDGI
                     KCDVEGNVYCTAPGGIYVHDASGKVLARIKTPGHHPTNIAWGDEEWRSLYITMIGSVV
                     RTRLNIAGVPSR"
     gene            613..975
                     /locus_tag="AC158Q_12035"
     CDS             613..975
                     /locus_tag="AC158Q_12035"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HIR3340547.1"
                     /translation="MEEEEITFKIRGCVFEVFRVLGAGFLEQVYQQALLYELEVQGLR
                     AEGQRALEVTYKGKPVGIYLADILVEGRIVLELKAVQSLTKAHEAQLLNYLKASGNRV
                     GLLVNFAHPKAEVRRFVL"
     gene            1045..1908
                     /locus_tag="AC158Q_12040"
     CDS             1045..1908
                     /locus_tag="AC158Q_12040"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020039.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR3340548.1"
                     /translation="MTWKFELVSGPFKGRTGGLAWDGAAMLFSAVQEERILRFDPATG
                     KTGVFRQYTGRTNGLAVAADGSVFGAQEGGRRVIHFLKDGSTAPTHELLDGRHHNQPT
                     DVIVDRGQRVWFTDPYNGTPPYGPPAYPFLDHASVLRLERGGTGGWTMKRVTHDTRGP
                     RAVLLSADEKTLYVADGDAERGDVCQLLAYPVGPDGSVGEGKSLLVFAPAERGIEGMC
                     LDAEGNIVACAGWRKGGAGPSVYVVSASGTLMETHPAPADLPMRCAFGDAGLASLYLT
                     AGDGGLYRATS"
     gene            2078..3778
                     /locus_tag="AC158Q_12045"
     CDS             2078..3778
                     /locus_tag="AC158Q_12045"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR3340549.1"
                     /translation="MPRMTGSRMFAEMLKGYGITHVFFVPTILMDALAEMDGLGIRKI
                     LTHGEKAAAYMADGYARARNGVGVCMAQQIGASNLAAGLRDPFMACSPVVAITGGPQT
                     GWRYRGAYQEVEDFSQFDSVTKLSLRVDDLSRFPDLVRQVFRTATTGAPGPVHLRLLG
                     SHGQGVEGEADLQPMAEPQFSRLPAFRPGPEMERVREAVAMLSRSQRPIIVAGGGVMA
                     SQAQAELVEIAEKLQIPVATSLTAKGAILDTHPLSIGVVGTYSRACANQAVAEADLVF
                     FIGAHAGGQITANWRIPAAGTPVIQLDINAAELGRNYPNAVSLVGDAKVTLRAMSDAA
                     QRKAPGDAKAWTGRVQQLAAEWRAGAEPMLKSDATPMRPERICREITQALPADGVVVT
                     DTGHAGMWTGQMIDMTQPGQRYIRCEGSLGWGLPGAMGVKCALPDRPVLCFTGDGGIY
                     YHLTELETAARHGINLVVVVNNNSSLNQEIPLVKTAYREKRDDRSGEIWRFQKNANLA
                     KVAEALGCAAFRVEKPGELKDLLPRAFAMGKPVVLDCISEEQALAPTAWLPGAGPRGH
                     "
     gene            3873..4868
                     /locus_tag="AC158Q_12050"
     CDS             3873..4868
                     /locus_tag="AC158Q_12050"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3340550.1"
                     /translation="MSMQLAVRRTAAALILPCSIGGAGVVSGAIAAEAYPSRPIRMVV
                     PFAPRGTSDVVARIIAEKLEPSLAQTVVIDNRPGAGGTLGAGITAQANPDGYTMLYAA
                     KSIAINASLYRNLPYDALRDLSAVTLVQETPYLFVVNDRVPVKSIKELIALAKAQPKK
                     LNYASGGAGTGPQMAMELLKLREGFDIVHVPYKGSAPALRDVVAGYVQVQCSSLIAAV
                     QFLGTGRLRPLAVSTAKRSPKVPDVPTMIESGIPGYDESGWSGILVPAGTSKAIVARL
                     NQAINATLREPAVVRSMELEGSTPHGSTPAEYSKRLVEEVRKWAEVIRRTGLRAN"
     gene            4909..>5148
                     /locus_tag="AC158Q_12055"
     CDS             4909..>5148
                     /locus_tag="AC158Q_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3340551.1"
                     /translation="MKPIRRVVTGNDANGRSRVLYDSAAPNVNPGAVRPGTCMTDVWV
                     FGTCPADISGERDDGRLPFSFEPPAAGGHLRIVQSP"
BASE COUNT          937 a         1620 c         1782 g          809 t
ORIGIN      
        1 ctcgtctacc tcggctacgg cgagatggga ctcaacccgc tgccgtgcaa ggtgaacgtg
       61 tcgatcgcgg tctggccgtg gggcgcgtac ctcggtgagg aagccctgaa gcacggcgtc
      121 cggatgaaga tctcgtcgtg gcgccgccac gatcccaacg ccctgccgcc tgcggccaag
      181 ggcacgggca tgtacctcaa ctcgtcgatg gccaagctcg aagccgtgaa ggccggcggc
      241 acgctgggca aggcgcggct cttccaccag tacaccggcc ccgagcgcgg cgttcccgac
      301 ggcatcaagt gcgatgtcga gggcaacgtc tactgcaccg cccccggcgg catctacgtg
      361 cacgacgcat cgggcaaggt gctcgcccgc atcaagacgc cgggccacca tccgaccaac
      421 atcgcgtggg gggacgagga gtggcgctcg ctctacatca ccatgatcgg ctcggtggtg
      481 cgcacccggc tcaacatcgc cggcgtgccg tcgcggtaag aaaacatcag ccacggaatc
      541 acactgaaaa cacggaatcg aaggaaagga ctcttggcca cggaaacaca cggaagcaca
      601 cggaaggcac acatggaaga ggaagagatt accttcaaaa tccggggctg cgtctttgag
      661 gttttccggg tgctcggcgc cgggttcctg gagcaggtgt accagcaagc gctcttgtac
      721 gagctggaag tgcaggggct gcgagcggaa ggccagaggg cgctggaagt cacctacaag
      781 ggcaaaccgg tcggtatata tctggcggat atccttgtag agggccgcat cgtcctggaa
      841 ttgaaggccg tacaatcatt gacgaaggcg cacgaggcgc aacttctcaa ctatctgaaa
      901 gccagcggca accgggtcgg gcttctcgtg aatttcgcgc atcccaaggc agaggtccgc
      961 cgctttgtac tatgaagctt tccgtgtgct tccgtgtgtt tccgtggctg aattgggtgt
     1021 attgcctcta agaaggaatc tgcaatgacc tggaaattcg aactcgtatc cggcccgttc
     1081 aagggaagga ccggcgggct ggcgtgggac ggagccgcga tgctcttcag cgcggtgcag
     1141 gaggagcgca tcctgcgttt cgacccggcg acggggaaga cgggcgtgtt ccgccagtac
     1201 acggggcgca ccaacggcct cgcggtggcg gccgacgggt cggtgttcgg cgcgcaggaa
     1261 ggcgggcggc gcgtcataca ctttctcaag gacggctcga cggcgccgac gcacgagctg
     1321 ctcgacggca ggcaccacaa tcagccgacc gacgtcatcg tcgaccgcgg acagcgcgtg
     1381 tggtttaccg atccctacaa cgggacgccg ccgtacgggc cgccggccta cccgtttctc
     1441 gaccatgcat cggtgctgcg cctcgagcgg ggcgggacgg gaggttggac gatgaagcgc
     1501 gtcacgcacg acacgcgcgg gccgcgcgcg gtgttgctgt ccgcggacga gaaaacgctg
     1561 tatgtcgccg atggcgacgc ggagcggggc gatgtgtgcc agctccttgc ctacccggtc
     1621 gggccggacg gcagcgtcgg cgaaggcaag tccctgctcg tgttcgcccc cgccgagcgc
     1681 ggcatcgagg gcatgtgcct cgatgccgaa ggcaatatcg tggcgtgcgc gggctggcgg
     1741 aagggcggcg ccgggccgtc ggtctatgtc gtatccgcct ccgggacgct gatggagacg
     1801 cacccggcgc ctgccgacct gccgatgcga tgcgcgttcg gggatgccgg cctggcaagc
     1861 ctttatctta cggccggcga cggggggctc taccgggcga cgagttagcc acggaaacac
     1921 acggaagggc acggaagact ttgccggaag agcgagattc cagattatct tgcattaccg
     1981 gcaacggttt gatgatcttg attcccgata ttgtcagtat gtttccgtgt gattccgtgg
     2041 ctaaattgaa ttttcgagca tagagaagga gcattacatg cccaggatga ccggatcgcg
     2101 gatgttcgcg gagatgctga agggctacgg catcacgcac gtgttcttcg ttcccacgat
     2161 actgatggat gccctcgccg agatggacgg cctgggcata cgcaagattc tcacgcacgg
     2221 cgagaaggcg gcggcctaca tggcggacgg ttacgcgcgc gcgcgaaacg gcgtgggcgt
     2281 gtgcatggcg caacagatcg gcgcctcgaa cctcgcggcc gggctgcgcg atccgttcat
     2341 ggcctgctcg ccggtggtgg ccatcaccgg cggaccgcag acgggatggc gctaccgcgg
     2401 cgcttaccag gaagtcgagg atttttccca gttcgactcg gtgacgaaac tgagcctgag
     2461 ggtcgacgat ctgtcccgct ttcccgacct tgtgcgccag gtctttcgca ctgcgaccac
     2521 gggtgccccg ggaccggtcc acctgcgcct gctcggctcg cacggccagg gcgtggaggg
     2581 cgaggccgac ctgcagccca tggcggagcc gcagttctcc cgcctgccgg catttcgccc
     2641 gggcccggaa atggagcgcg tgcgcgaggc ggtcgcaatg ctgtcgcgct ctcagcgccc
     2701 gatcatcgtc gccggcggcg gcgtgatggc gtcccaggcg caggccgagc tggtggaaat
     2761 tgccgagaaa ctccagatcc ccgtggcgac ttcgctcacc gccaagggcg ccatcctcga
     2821 cacccacccg ttgtccatcg gcgtcgtcgg cacctactcc cgcgcgtgcg ccaaccaggc
     2881 ggtggcggaa gccgacctgg tgttcttcat cggcgcccat gcgggcgggc agattaccgc
     2941 caactggcga ataccggccg cgggcacccc ggtgatccag ctcgacatca acgccgcgga
     3001 actgggcagg aattacccca acgccgtatc gctcgtcggc gacgccaaag tcacgctgcg
     3061 ggcgatgtcc gacgccgcgc agcgcaaggc ccccggggac gcgaaagcgt ggaccgggcg
     3121 cgtgcaacaa ctcgccgcgg agtggcgcgc gggggcggag ccgatgctga aatcggatgc
     3181 gacgccgatg cggccggagc gaatctgccg ggagattacc caggcgctgc ccgcggatgg
     3241 cgtggtggtt accgacacgg gacacgccgg catgtggacc ggacagatga tcgacatgac
     3301 gcagccgggg cagcgctaca tccgctgcga aggatcgctc ggatggggac tgcccggcgc
     3361 gatgggcgtg aaatgcgcgc tgcccgaccg gccggtgctg tgcttcaccg gcgacggcgg
     3421 catctactac cacctgacgg agctcgaaac cgccgcgcgc cacggcatca atctcgtcgt
     3481 ggtggtgaac aacaacagct cgctcaacca ggagattccc ctggtcaaga cggcgtaccg
     3541 cgagaagcgc gatgaccgtt ccggcgaaat ctggcgcttc cagaagaacg cgaacctcgc
     3601 aaaggtcgcg gaagcgctgg gctgcgcagc attccgcgtg gaaaagccgg gcgaactgaa
     3661 ggacctgctg ccgcgagcgt tcgcgatggg caagccggtc gtgctggact gtatcagtga
     3721 agagcaggcg ctcgccccga ccgcgtggct gccgggggcc ggcccgcgcg ggcattgacc
     3781 tggccgccgg cgcgcattgc gcgcccggaa aaacggacac ggaatgcccg gcgtggagtc
     3841 tcggcagtcc gattgatacc caggagggca tcatgagcat gcaactggca gttcgccgca
     3901 ccgcggcagc attgatattg ccctgcagta tcggtggggc gggcgtggtc tccggcgcga
     3961 tcgcggcgga ggcatatccg tcgcggccga tcagaatggt ggttccgttt gccccgcgcg
     4021 gaacgagcga cgtggtggcg cggatcatcg ccgagaagct cgagccctct cttgcgcaaa
     4081 cggtggtcat cgacaaccgc cccggcgccg gcggcaccct gggcgcgggg atcacggcgc
     4141 aggcgaatcc ggacggttac acgatgctgt acgccgcgaa gagcatcgcc atcaacgcga
     4201 gcctgtaccg caaccttccg tacgacgcgc tgcgcgacct gtcggccgtc accctggtcc
     4261 aggagacgcc gtacctgttc gtggtcaacg accgggtgcc ggtcaagtcg atcaaggagc
     4321 tgatcgccct cgcgaaggcc cagccgaaga agctcaacta tgcgtccggc ggagccggga
     4381 ccggaccgca gatggccatg gagctgctga agctgagaga gggcttcgac atcgtgcacg
     4441 tgccgtacaa ggggtccgcg ccggccctgc gcgacgtcgt ggcaggctac gtgcaggtgc
     4501 agtgttcgag cctgattgcc gcggtgcagt ttctgggcac ggggcggctg cgccctctcg
     4561 cggtcagcac cgcgaaacgc tcgccgaagg tgcccgacgt tccgaccatg atcgagtcgg
     4621 gcattcccgg ctacgatgaa agcggctggt cgggaatcct ggtgccggct ggaacgtcga
     4681 aagcgatcgt cgcgcggctg aaccaggcca tcaacgcgac cctgcgcgag cctgctgtcg
     4741 tgcgcagcat ggaactggag ggatccacgc ctcacggcag cacgcccgcg gagtactcga
     4801 agcgcctggt ggaggaagtg aggaagtggg cggaggtaat caggcggacg ggtctgcggg
     4861 ccaattaaat cgcaagcgcg cgacggataa cggcgcatgg cccaaccggt gaaacccata
     4921 cggcgcgtcg tgacgggaaa cgacgcgaac ggaaggtcgc gggtgctgta cgacagcgcc
     4981 gcgccgaacg tcaacccggg cgcggttcgg cccggcacgt gcatgacgga cgtatgggtc
     5041 ttcgggactt gccccgcgga catctccggc gagcgcgacg atggccggct accgttcagc
     5101 ttcgagccgc ccgccgccgg tggccatctg cgcatcgtgc aatcgccg
//
LOCUS       DBNGSG010000461         2488 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3375635, whole genome shotgun sequence.
ACCESSION   DBNGSG010000461 DBNGSG010000000
VERSION     DBNGSG010000461.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3375635"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..553
                     /gene="rplS"
                     /locus_tag="AC158Q_12060"
     CDS             179..553
                     /gene="rplS"
                     /locus_tag="AC158Q_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HIR3340552.1"
                     /translation="MSAVDKVTAAQLRQRPAFRPGDTIRVHVRITEGDKTRVQPFEGV
                     VIGCRGRGIQETFTVRKVSFGIGVERIFPAHSPAIERIDVVRGGKVRRAKLYYLREKK
                     GKKASRIKEAERRVETEAPAGQ"
     gene            complement(558..1346)
                     /locus_tag="AC158Q_12065"
     CDS             complement(558..1346)
                     /locus_tag="AC158Q_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340553.1"
                     /translation="MRLRPAALFVLLMIPLTLPAQVRVEVPPAAKINVTYTEPADAGQ
                     ADRLREVAARVKGPADQLDPLTEISLVIVHSQRELDQRLGPEAEGRLAGVSYVHGILF
                     LSPNTWARTPTGEAIDHEMQEALIRYNVERLAGGNRIPFWLEDGLVSVLGKRPFAPAS
                     AEPVAQRGALLLVRFEPDDPAVGYWAVRYLIEQRGGLTSLRQLLRLSAQRPDSFVENL
                     QLTYGMSVGELEREWRKWLQALVDADKRQRETGVKEGPLVKPRE"
     gene            1409..2110
                     /locus_tag="AC158Q_12070"
     CDS             1409..2110
                     /locus_tag="AC158Q_12070"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF014244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR3340554.1"
                     /translation="MEPEKLLLGVVWYAVFLLSTTCHEAAHAWVAKLGGDLTAFHEGQ
                     VSLSPLPHIRREPFGTVLVPIGSYLLGGWMIGWASAPYDPVWAERHPRRAALMALAGP
                     LANLTLVLLAAVGIRTGLALGAFIAPESINFTTVVSAAEPGLTSALATLLSILFVLNL
                     LLFLFNLLPVPPLDGSSAVTLLMSEETGRRFREFTHSWGIVGLLLSWYLFRVIFSPLF
                     TLALNILYFPIASYG"
     gene            2177..2458
                     /locus_tag="AC158Q_12075"
     CDS             2177..2458
                     /locus_tag="AC158Q_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006401987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HIR3340555.1"
                     /translation="MAERMVKCAKFGETLPGLDEPPYTGELGQRIYENVSKKAWDLWK
                     EHVKMLINEYQLNPATPEAWQIISSEAEKLFFGEGAQLPPGYVPPQQKK"
BASE COUNT          440 a          817 c          778 g          453 t
ORIGIN      
        1 ctccggcatc cgggaggtcg accgcgaaac cctgaagacc gtccgtaagt ggaaatacaa
       61 accaaggcct gggtgtcaag ctgtcgaagt cggaatgacg atcgacatcc acgtctacta
      121 gccattagca ggaaaatcgg ctatactgca atttcgtttt tcttcaggga gaaatccgat
      181 gtcggctgtg gacaaagtta ccgccgcgca actgcgccag cggcccgcct tccggccggg
      241 ggacaccatc cgtgtgcacg tgcgcatcac ggaaggcgac aagacccgcg tgcagccctt
      301 cgagggcgtg gtcatcggct gtcgcggccg cggcatccag gaaactttca ccgtgcgcaa
      361 ggtctcgttc ggcatcggag tggagcgcat cttccccgcc cactcgcccg ccattgagcg
      421 catcgatgtg gtgcgcggcg gcaaggtgcg ccgcgccaag ctctattacc tgcgtgagaa
      481 gaagggtaag aaggccagcc gcatcaagga agccgagcgg cgcgtagaaa ccgaagctcc
      541 cgccggtcaa taagccgcta ctcgcgcggc ttcaccagcg gtccttcctt cactcccgtt
      601 tcgcgttgcc gcttgtcggc gtccaccagc gcctgcagcc acttgcgcca ttccctctcc
      661 agctcgccga cagacatccc gtaggtcaat tgcaggtttt cgacgaagga gtcggggcgc
      721 tgggcgctca accgcagcaa ttgccgcagc gaggtcaggc cgccgcgctg ctcgatgagg
      781 tagcgcaccg cccagtagcc caccgccggg tcgtccggtt cgaaacgcac cagaagtagc
      841 gcgccgcgct gcgccaccgg ctcggcgctg gccggcgcga agggccgctt gcccagcacg
      901 ctcaccaatc catcctccag ccagaacgga atccggttgc cgcccgccag gcgctccacg
      961 ttgtagcgga tgagcgcctc ttgcatttca tggtcaatgg cttcgccggt gggggtgcgc
     1021 gcccaggtgt tgggggaaag aaagagaatt ccgtggacgt agctgacgcc ggccaaccgg
     1081 ccttcggcct caggcccgag ccgctggtcg agctcgcgct gcgagtgcac gatgaccaga
     1141 ctgatttcgg tcagcgggtc gagctggtcg gcgggacctt tgacgcgcgc tgccacctcg
     1201 cgcagccggt cggcctgccc ggcgtcggcg ggctcggtgt aggtgacgtt gatcttggcc
     1261 gccggcggca cttccacgcg cacctgggcg ggcagcgtca acggaatcat cagcaggaca
     1321 aacagcgcag cgggtcgcag ccgcatggtt cccctcccct ctgtgatact atcgcgcttc
     1381 gattcgcgcg tcagcggaaa agttttccat ggagccggaa aaactccttc tcggtgtggt
     1441 ctggtacgcg gtcttcctgc tgtcgaccac ctgccacgag gccgcgcatg cctgggtggc
     1501 caagctgggc ggcgacctga cggcgttcca cgagggtcag gtatcgctca gcccgctgcc
     1561 gcacatccgc cgcgagccct tcggcaccgt gctggtgccc atcggctcct atctcctggg
     1621 cggatggatg atcggctggg ccagcgcgcc gtacgatccg gtgtgggccg agcggcaccc
     1681 gcggcgggcg gcgctgatgg cgctggcggg cccactggcc aacctgacgc tcgtcctgct
     1741 ggccgcagtg gggattcgca ccgggctggc gctgggcgcg ttcatcgcgc cggagagcat
     1801 caacttcacc accgtggtca gcgccgccga gcccggcctg acctcggcgc tggcgacgct
     1861 gctgagcatc ctgtttgtgc tcaacctgct gctgtttctt ttcaacctgc ttcccgtgcc
     1921 gccgctcgac ggcagctccg ccgtcaccct gctgatgagt gaagaaaccg gccggcgttt
     1981 ccgcgagttc actcattcct ggggtatcgt cgggctgctg ctgtcgtggt atctctttcg
     2041 agtaatcttc agcccgttgt ttacactggc gctgaacatc ctctacttcc ccatcgccag
     2101 ctacggctag taggtggcgg gaaaggctga attcgaatag actgcgggag ttcaaacgct
     2161 ccttcgagag gacacaatgg cggaacggat ggtgaagtgc gcgaagttcg gcgaaaccct
     2221 gccggggctg gacgagccgc cctacaccgg cgagctgggc cagcgcatct atgaaaacgt
     2281 ctccaagaaa gcctgggacc tctggaaaga gcacgtcaag atgctcatca acgaatacca
     2341 actcaacccc gccacgcctg aagcctggca gattatttcg tcggaagcgg agaagttatt
     2401 cttcggtgaa ggggctcagc tcccgcccgg ttacgtccca ccccagcaga agaaataacc
     2461 gtccgggctt gagtcggttc aactccac
//
LOCUS       DBNGSG010000462         4307 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_489463, whole genome shotgun sequence.
ACCESSION   DBNGSG010000462 DBNGSG010000000
VERSION     DBNGSG010000462.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4307)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_489463"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="AC158Q_12080"
     CDS             <1..659
                     /locus_tag="AC158Q_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HIR3340556.1"
                     /translation="DITSINLITAGNLVEATQDAGSFVQLSGVLKRVAVKLSKTCNDL
                     RLLSSGPRAGFNEINLPAVQAGSSIMPGKVNPVIPEVVNQIAFEVIGNDVTVSFAAEA
                     GQLQLNAFEPVIAHSLFKGLKHLANGCDTLRERCVKGVTANRDHLRRTVENSIGIVTA
                     LNPYIGYANATRIAQEAHATGRSVYDLVLEKQLLSKERLDEILRPEVLTRPNYVPEAK
                     "
     gene            complement(772..1641)
                     /locus_tag="AC158Q_12085"
     CDS             complement(772..1641)
                     /locus_tag="AC158Q_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3340557.1"
                     /translation="MKPSRSEFHEIRGLRYHVRTWGDPGAPALFLLHGWMDVSASFQF
                     LVDCFSREWHVIAPDWRGFGLSGWAREGYWFPDYYADLEALLDLYQPAAPARIVGHSM
                     GGVIGSVYAGIRPDRVARLVSMEGLGLARHTPDQAPVRYEQWLDQLRDPPAFMPYRSF
                     DQVAARLKKNNPRLPDERAAFLASHWAKQLDSGEVVLLSDPRHKTLNPYLFRIEEAIA
                     CWRRVTAPVLLVSGKLSEIPARMKDNPGQLAERKGAFRNRHEIELEDAGHMMHHDQPE
                     KLASLLEDFLTAG"
     gene            1821..2738
                     /locus_tag="AC158Q_12090"
     CDS             1821..2738
                     /locus_tag="AC158Q_12090"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014066.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HIR3340558.1"
                     /translation="MLSFHIPDLMTLRARFAAGFALLAALAAVAVPAPAAERFEQGLL
                     WRVEGTGAPPSHVFGTIHLADPRVTALPPVVAREFEQARSVVLEARLDGAGFQLLAGR
                     MLYTDGRDLAGVAGEDLFRKAVAVAGGAGLPEPALRLLKPWAVAVLLSVPAQNPADVL
                     DFVLARLAMERGKPLHELENLEEQISVFEGMSETEQVMLLREAVDNYESMPRLIGRVI
                     EAYLARDLAGIWRIREESSGKSREARRFNETFMRRLLHDRNARMAERAEPLLREGGAF
                     IAVGALHLYGSGGVPALLERRGYRVTRVY"
     gene            2889..>4307
                     /gene="dnaE"
                     /locus_tag="AC158Q_12095"
     CDS             2889..>4307
                     /gene="dnaE"
                     /locus_tag="AC158Q_12095"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR3340559.1"
                     /translation="MAPSFVHLRLHSEYSIVDGIVRIDEAVEAAVADGMPALGLTDLS
                     NVFGLVKFYQEARAKGVKPVIGCDLWLENEADRDKPYRMLLLCQSRAGYLKLCDLLTR
                     AYRANQYRGRPEMKKTWLAQAGTDGLIALSGAHHGDVGQALATDNAKQAQRLAREWHE
                     IFPGRYYLEVQRLGKNAAVVGASGVPVETCVRRSLALASKMKLPVVATHPVQFVKRGD
                     FRAHEARVCIAQGYTLSDQRRPKLFSQEQYFKTQAEMAQAFGDIPQVLANSMEIAKRC
                     SLEIELGKSRLPLFQTPNNVSLDDYLRIRAAEGLEQRLPQLFPDAAQRAREAPRYHER
                     LGFEIKTIQQMGFPGYFLIVADFINWAKGNGVPVGPGRGSGAGSLVAYSLGITDLDPL
                     RYDLLFERFLNPERVSMPDFDIDFCQDGRDRVIDYVKAKYGADSVSQIVTFGTMAAKA
                     VVRDVGRVLDMPYNFCDQLAKLI"
BASE COUNT          753 a         1375 c         1436 g          743 t
ORIGIN      
        1 cggacatcac gtccatcaac ctcatcacgg cgggcaatct ggtggaggcg acgcaggacg
       61 ccggcagctt cgtccagctc tcgggcgtgc tgaagcgcgt ggcggtcaag ctgtccaaga
      121 cctgcaacga cttgcggctg ctttcctcgg ggccgcgcgc ggggttcaac gagatcaacc
      181 tgccggcggt gcaggcgggt tcttccatca tgccggggaa ggtcaacccg gtcatacccg
      241 aagtggtcaa ccagatcgcc ttcgaggtga tcggcaacga cgtgacggtc tccttcgccg
      301 ccgaggcggg ccagctgcag ttgaacgcct tcgagccggt cattgcccac agtctgttca
      361 agggcctgaa acacctggcg aacggctgcg acacactgcg ggaacgctgc gtgaagggcg
      421 ttaccgccaa ccgcgatcat ctgcgccgca ccgtcgagaa ctcgatcggc atcgtcaccg
      481 cgctcaaccc gtatatcggt tatgccaatg cgacccggat agcgcaggag gcgcatgcca
      541 ccggcaggag cgtctacgac ctggtcctgg aaaaacagct gctgagcaag gagcggctcg
      601 acgagatcct ccggcccgaa gtgctcacgc gacccaatta cgtgcccgag gcaaagtgat
      661 gcgaggaaat tcgggataag ggatggggga taggcctccc ccgctgatgc ggggtgtttg
      721 actgccgctg cgtcattccg cgcggtgcgc gggtgcctta tcccttatcc ctcatcccgc
      781 agtaaggaaa tcctccagta gggacgccag cttttcgggc tgatcgtgat gcatcatgtg
      841 gccagcgtcc tcgagttcga tctcgtgccg gttgcggaag gcgcccttgc gctcggcaag
      901 ctgccccggg ttgtccttca tgcgcgccgg gatttccgac agttttcccg ataccagtaa
      961 cacgggagcg gtaacgcgcc gccagcaggc gatcgcctcc tcgatgcgga acaggtacgg
     1021 attgagggtc ttgtggcgcg ggtcgctcag cagcacgacc tcgcccgaat cgagctgttt
     1081 cgcccagtgg cttgcaagaa acgccgcccg ctcgtccggg agcctggggt tgttcttctt
     1141 caggcgggcg gcgacctggt cgaacgagcg atagggcatg aacgctggcg gatccctgag
     1201 ctggtcgagc cactgctcgt agcgcaccgg cgcctggtcg ggcgtatgcc gggcaagacc
     1261 caggccctcc atcgacacca atcgcgcaac gcggtccggc cggatgccgg cgtagacgga
     1321 gccgatgacg ccgcccatgc tgtggccgac gatccgggcg ggcgcggccg gctgatagag
     1381 gtcgagcagc gcctccaggt cggcgtagta atcgggaaac cagtaacctt cgcgcgccca
     1441 cccggagagc ccgaagccgc gccagtccgg agcgatgacg tgccattcgc gcgaaaaaca
     1501 gtccaccagg aactggaacg acgccgaaac gtccatccag ccgtgcagca ggaacagcgc
     1561 gggcgcgccg ggatcgcccc aggtgcgcac gtgataacgc aacccgcgga tctcgtgaaa
     1621 ctcggaacgg ctcggtttca tacccttcta atatcgttac tcgttactcg ttactcgtga
     1681 atcttgaaaa cgcggattgt gcccgcgcga tgaacggggg cgctccgagc gctcgaacgc
     1741 gaggaactga accggtgctt ccgcatccag attcaacaac tcttgctatc atgccgggac
     1801 ggcgcgcgat tctacccaaa ttgctgtcat tccacatccc cgatctcatg acgttgcgtg
     1861 cacgctttgc ggctgggttc gctttgctcg ccgcgctggc ggccgtggcc gtacccgcgc
     1921 cagccgcgga gcgtttcgag caggggctgc tgtggcgcgt ggaaggcacg ggcgcgccgc
     1981 caagccacgt gttcgggacc atacacttgg ccgacccgcg cgtgactgcg ctgccgccgg
     2041 tggtggcgcg tgagttcgag caggcgcgca gcgtcgtgct cgaagccagg ctggatggcg
     2101 ccgggtttca gttgcttgcc ggccgcatgc tctacaccga tggccgcgac ctcgcgggcg
     2161 tggcgggaga agacctgttc cggaaggccg ttgcggtggc gggcggggcg ggcctgcccg
     2221 agccggcgct gcggctgctc aagccctggg cggtggccgt gctgctgagc gtgcccgcgc
     2281 agaacccggc cgacgtgctg gatttcgtgc tggcgcgcct tgccatggaa cggggcaagc
     2341 cgctccatga gctggagaac ctggaagagc agatttccgt attcgaggga atgtccgaaa
     2401 cggaacaggt gatgctgctg cgggaggcgg tggacaatta tgagagcatg ccgcgcctga
     2461 tcggacgcgt gatcgaggcc tacctcgcgc gcgatctcgc cggcatctgg cgcatccggg
     2521 aggaaagcag cggcaagagc cgggaagcgc ggcgattcaa cgagaccttc atgcggcgcc
     2581 tgctgcacga ccgcaacgcc cgcatggccg agcgcgcgga accgctgctg cgggagggcg
     2641 gcgcgttcat cgcggtcggc gcgcttcacc tgtatgggag tggtggcgtg ccggcgctgc
     2701 tcgagcgccg cggctaccgc gtcacgcggg tctattgaaa gcgtgattcg tgaatcgtga
     2761 ttggtgattg gccccgaaaa ccgccgcctt tggccttctc cgggaaaacg agcccgaatc
     2821 acgacttacg attcaagaat cacgaatcac gcttctcagt tgcccgcggc gcgccgtaaa
     2881 ataggcgcat ggctccgtcg ttcgttcatc tgcgattgca cagcgagtat tcgatcgtgg
     2941 atggcattgt ccgcatcgac gaggcggtcg aggcggcggt ggccgacggc atgccggcgc
     3001 tcgggctgac cgatctctcc aacgtcttcg gcctggtcaa gttctaccag gaggcgcgcg
     3061 cgaagggtgt gaagcccgtc atcggctgcg atctgtggct ggagaacgaa gccgatcgtg
     3121 acaagcccta ccgcatgctg ctgctgtgcc agtcccgggc cggttacctg aaattgtgcg
     3181 atctcctcac ccgtgcctac cgcgccaacc agtaccgcgg ccggcccgag atgaagaaga
     3241 cctggttggc gcaagccggc actgacggcc tgatcgcgct ctccggggcg caccacggcg
     3301 atgtggggca ggcgctggcc acggacaatg cgaagcaggc gcagcggctg gcccgcgaat
     3361 ggcacgagat tttccccggg cgctattacc ttgaggtgca gcgcctcggg aagaacgcgg
     3421 cggtcgtggg ggcatcgggc gtgccggtcg agacctgtgt tcggcgctcg ctcgcgctcg
     3481 cctcgaagat gaagctgccg gtggtggcga cgcacccggt tcaattcgtg aaacgcgggg
     3541 attttcgcgc gcacgaagcg cgcgtctgca tcgcccaggg ctacacgctg tccgaccagc
     3601 gccggccgaa actgttcagc caggagcagt acttcaagac gcaggccgag atggcccagg
     3661 cgttcggcga tattccccag gtgctcgcca acagcatgga gatcgccaag cgctgcagct
     3721 tggagatcga gctgggcaag tcccgcctgc cgctgttcca gacgccgaat aacgtcagcc
     3781 tcgacgatta cctgcgcatc cgcgccgccg aagggctcga gcagcggctg ccgcaactct
     3841 ttcccgatgc cgcgcagcgt gcgcgcgagg cgccccgtta tcacgagcgg ctcggcttcg
     3901 agatcaagac catccagcag atgggcttcc cgggctactt cctgatcgtc gccgatttca
     3961 tcaactgggc gaaaggaaac ggcgtcccgg tgggcccggg acgcgggtcc ggcgccggct
     4021 cgctggtggc atactcgctc ggcatcaccg atctcgaccc gctgcgctac gacctgctgt
     4081 tcgagcgttt cctgaatccc gagcgtgtgt cgatgccgga cttcgacatc gacttctgcc
     4141 aggacgggcg cgaccgggtg atcgactacg tgaaagccaa gtacggcgcc gacagcgtct
     4201 cgcagatcgt caccttcggc accatggccg cgaaggcggt ggtgcgcgac gtcgggcgcg
     4261 tgctggacat gccctacaac ttctgcgacc agctcgccaa gctgatc
//
LOCUS       DBNGSG010000463         6139 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1169068, whole genome shotgun sequence.
ACCESSION   DBNGSG010000463 DBNGSG010000000
VERSION     DBNGSG010000463.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6139)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6139)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6139
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1169068"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="AC158Q_12100"
     CDS             <1..270
                     /locus_tag="AC158Q_12100"
                     /inference="COORDINATES: protein motif:HMM:NF012787.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR3340560.1"
                     /translation="QQRYGARGLTFVGIAIDQPDKVAEFAREFRINYPLLVGGPDTLD
                     LLRQVGNRAGVLPYTLVIDRGGKLVTREPGGLKEARLEALLQPLL"
     gene            538..1026
                     /gene="aroQ"
                     /locus_tag="AC158Q_12105"
     CDS             538..1026
                     /gene="aroQ"
                     /locus_tag="AC158Q_12105"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HIR3340561.1"
                     /translation="MAADSTQLTMAGAKNILVINGPNLNLLGSRETQHYGKATLPAIN
                     ARLASRARKAGVRLATYQSNSESELVDRVQQARRDKVDFIVINPAAYTHTSVALRDAL
                     AAVGIPFVEVHLSNVFSRESFRRESYFTDIAVGIISGLGAQGYELALEFALQFSARKK
                     KG"
     gene            1031..1495
                     /gene="accB"
                     /locus_tag="AC158Q_12110"
     CDS             1031..1495
                     /gene="accB"
                     /locus_tag="AC158Q_12110"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HIR3340562.1"
                     /translation="MDLRKLKTLIDLVQQSGIAELEITEGEERVRIARGGSGTQPAAP
                     VPVYTLPQAAGAAPAPAAGAPAAPPAEADAAPEGHAIRSPMVGTFYRSAAPGAKAFVE
                     VGQAVKAGDTLCIIEAMKLLNEIEADQDGTVKAILAENGQPVEYGEPLFILG"
     gene            1619..2974
                     /gene="accC"
                     /locus_tag="AC158Q_12115"
     CDS             1619..2974
                     /gene="accC"
                     /locus_tag="AC158Q_12115"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR3340563.1"
                     /translation="MFEKILIANRGEVALRIQRACREMGIKTVVVHSEADREAKYVKL
                     ADESVCIGPPPSAASYLNIPAIISAAEVTDAEAVHPGWGFLSENADFAERVAQSGFVF
                     IGPRADTIRLLGDKVNAKAAMKKAGIPVVPGGEGPLPQEPNDIIKIARTIGYPVIVKA
                     SGGGGGRGMRVVHTEAALLTAVQTTRTEAQAAFGNPAVYLEKYLENPRHIEFQVLADR
                     FRNAIHLGERDCSMQRRHQKVIEEAPALHLNSRSRIRTAERCAEACHKLGYEGAATFE
                     FLYENGEFYLIEINTRLQVEHPVTEMITGIDIVQQQIRIAAGEKLRIRQRDVQFRGHA
                     IECRINAEDPYRFTPSPGRITSYHMPGGPGIRVDSHAYNGYFVPPYYDSLIGKIIAYG
                     DTREQAIARLSIALSETAIEGIKTNIPLHQELLLDAAFLRGGTNIHYLEQRLARLARD
                     E"
     gene            3072..3965
                     /gene="prmA"
                     /locus_tag="AC158Q_12120"
     CDS             3072..3965
                     /gene="prmA"
                     /locus_tag="AC158Q_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HIR3340564.1"
                     /translation="MSWISVSFEVAAAEVEAVSDALLEAGAASVDVADADAGSASERP
                     VFAEPGGDAPLGWRRSLVTALAAEGTDAAALVAAACARAGIAPPPHRARRVAEQDWVR
                     ASRDQITPIRVSQRLWIVPSWHAPPDPGAINLLLDPGLAFGTGSHPTTRLCLEWLERT
                     VRGGETVLDYGCGSGILAIAAMKLGAARADGVDIDPQALLAARANALHNRVQATFHGA
                     ADAIQQPAQIVVANILAHPLIVLAPLIARLTARGGRLALAGLLAGQEDGVRAAYEPWF
                     DFEAPARDEGWMLLAGARRAA"
     gene            3962..4795
                     /locus_tag="AC158Q_12125"
     CDS             3962..4795
                     /locus_tag="AC158Q_12125"
                     /inference="COORDINATES: protein motif:HMM:NF023334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon and DUF3426 domain-containing
                     protein"
                     /protein_id="HIR3340565.1"
                     /translation="MSLVTKCAHCGTTFRVTQPQLQAHNGRVRCGTCMTVFDGLKELV
                     TLPEPAPETSPKTAAGAGDVAGFELEPVVPAAITTAPAIPVPPEAVHEERDYGPVPEQ
                     LSLDDQLYPEEARRRARWWAVGTALLAFVLAGQAVYFHRADLSAQYPGLKPYLVRFCG
                     LLRCTVSPPQRPKQIAIEASDLQALDAARPQIIQLTATLRNHAGYDLAFPVLDLVLTN
                     TKEHTLARRLFTPAEYLERGRDVMAGIPANAEITVRLDLDAGDLNPAGFRLDLLPAPQ
                     P"
     gene            4840..5226
                     /gene="gcvH"
                     /locus_tag="AC158Q_12130"
     CDS             4840..5226
                     /gene="gcvH"
                     /locus_tag="AC158Q_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HIR3340566.1"
                     /translation="MNLPEDLRYTAEHQWVRMEADGTAAVGITDHAQEQLGDVVFVQL
                     PEAGRKVKRGETCAVVESVKAASDIPAPLSGEIVAANPAAADAPEKLNHEPYAAWLFR
                     IRPGVAAELTGLLDAEAYSRTVMSDK"
     gene            5333..5833
                     /gene="tpx"
                     /locus_tag="AC158Q_12135"
     CDS             5333..5833
                     /gene="tpx"
                     /locus_tag="AC158Q_12135"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HIR3340567.1"
                     /translation="MANVTLGGNPITVAGALPKKGDSAPDFTLTARDLKDMGLGDFAG
                     KRKVLNIVPSLDTPVCAKSTRVFNEKAGALPNTVVLVISADLPFAAGRFCTAEGIDNV
                     VTLSTMRGGDFKTKYGVDITSGPLKGATARAVIVLDAGNKVLHSELVPEIKQEPDYDA
                     ALAVLN"
     gene            5934..>6139
                     /locus_tag="AC158Q_12140"
     CDS             5934..>6139
                     /locus_tag="AC158Q_12140"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HIR3340568.1"
                     /translation="MRRIWNAFFYSLAGLAAAFRHEDAFRQEVFLGAILIPAAFFVPA
                     NGAGRALMVGAVLLVLIVELLNSAV"
BASE COUNT         1109 a         2036 c         2007 g          987 t
ORIGIN      
        1 cagcagcgct acggcgcccg cggactgacg ttcgtcggca tcgccatcga ccagccggac
       61 aaggtcgccg agtttgcccg cgagttccgg atcaactacc ccctactggt gggaggaccg
      121 gacacgctgg acctgttgcg acaggtcggt aaccgggccg gcgtgctgcc ctataccctg
      181 gtaatcgacc gggggggcaa actggtgact cgggagccgg gcgggctcaa ggaagccagg
      241 ctcgaggccc tgttacagcc cctgctatag gctattactc ccagttatac ggcctaattc
      301 cgcctaactt ctgttaactc gttgctagta atccgcagtt gttttccagg taaatccggc
      361 gcgccgaagg ctgcggagat ccaagctcgg ctcgtggcta agcaaccgga tttcaggggt
      421 attttgggtt tatttccccg gtaacttcgc ttaatttata gacaactgtc aggattttgg
      481 gcaaactcgc gcccatcctc ctcctcgact acggatcagc cggcatttcc gctggtaatg
      541 gccgcagatt ccacccaact caccatggct ggcgccaaga acatcctggt catcaacggc
      601 cccaatttga atcttctcgg gtcgcgcgag acgcagcact acggcaaagc caccctgccg
      661 gcaatcaacg cccggctggc cagccgcgcc aggaaagccg gggtgcggct cgcaacctat
      721 cagagcaatt ccgaatcgga actggtggac cgggtgcagc aggcccggcg ggacaaggtg
      781 gacttcatcg tcatcaatcc cgccgcttat acccacacca gcgtggcgct gcgcgatgcg
      841 ctggcggcag tgggcattcc ctttgtcgaa gtgcacttgt cgaacgtgtt ttcccgggaa
      901 tccttccgcc gcgagtcgta cttcaccgac atcgcggtcg gcatcatcag cgggctcggc
      961 gcccagggct acgagctcgc cctggaattc gccctgcagt tttcggccag gaagaaaaag
     1021 gggtaaggcc atggatttgc gcaagctgaa gaccctgatc gatctcgtgc agcagtcggg
     1081 catcgcggag ctcgaaatca ccgagggcga ggagcgggtg cgcatcgccc ggggcgggag
     1141 cggcacgcag ccggccgcac cggtgccggt ctacacgctg ccgcaagccg cgggagccgc
     1201 gcccgccccg gccgccgggg cgccggccgc tccgccagcc gaagccgatg ccgcgcccga
     1261 gggtcacgcc atccgctccc ccatggtcgg cactttctac cgctccgccg cccccggcgc
     1321 aaaagcgttc gtcgaggtcg ggcaggcggt caaggcaggc gacacgttgt gcatcatcga
     1381 ggcgatgaag ctcctgaacg aaatcgaagc cgaccaggac ggcacggtga aggccatcct
     1441 ggcagagaac gggcagccgg tggagtatgg cgagccgctg tttatcctcg gctgatcgcc
     1501 ggggaaggcg tgaatggtga ttggtgaatg gtgaatagac gaaacgccgg ctggcgcgca
     1561 gcgcttcgcg aactattccc ctcacccttc accattcatc attcacgcag ttcatccgat
     1621 gtttgagaag atactcatcg ccaaccgcgg cgaagtcgcg ctgcgcatcc agcgcgcctg
     1681 ccgcgagatg ggcatcaaga cggtggtggt gcactcggag gcggaccgcg aggccaagta
     1741 cgtcaagctc gccgacgagt ccgtgtgcat cggcccgccg ccttccgccg ccagctatct
     1801 caacataccc gccatcatca gcgccgcgga agtgacggac gccgaggcgg tccatcccgg
     1861 ctggggtttc ctgtcggaga acgccgattt cgccgagcgc gtcgcgcaga gcggcttcgt
     1921 gttcatcggc cccagggccg acaccatccg gttgctgggc gacaaggtga acgccaaggc
     1981 cgcgatgaaa aaggccggca tcccggtagt gccgggcggc gaggggcccc tgccccagga
     2041 accgaacgac atcatcaaga tcgcgcgcac gatcggctac ccggtgatcg tcaaggcgtc
     2101 gggcggcggc ggcgggcgcg gcatgcgcgt ggtgcacacc gaggcggcgc tactgacggc
     2161 ggtgcagacc acgcgcaccg aagcgcaggc cgctttcggc aatcccgcgg tctacctcga
     2221 gaagtacctg gagaacccgc gccacatcga gttccaggtg cttgccgacc gtttccgcaa
     2281 cgcgatccac ctgggcgagc gcgactgctc gatgcagcgg cgccaccaga aggtgatcga
     2341 ggaggcgccc gcgctgcatc tcaactcgcg cagccgcata cgcaccgccg agcgctgcgc
     2401 cgaagcctgc cacaagctcg gttacgaagg cgccgccacg ttcgagttcc tctatgagaa
     2461 cggcgagttc tacctgatcg agatcaacac gcggctgcag gtcgagcacc cggtgacgga
     2521 gatgatcacg ggcatcgaca tcgtgcagca gcagatccgc atcgcggcgg gagagaagct
     2581 gcgcatccgg cagcgcgatg tgcagttccg cgggcacgcc atcgagtgcc gcatcaacgc
     2641 ggaggacccg taccgcttca cgccttcgcc cgggcgcatc acgtcgtacc acatgcccgg
     2701 cggtcccggc atccgggtgg actcgcacgc ctacaacggc tacttcgtgc cgccgtacta
     2761 cgattccctg atcggcaaga tcatcgccta cggcgacacc cgcgagcagg ccatcgcgcg
     2821 cttgagcatt gcgctctcgg agaccgcgat cgagggcatc aagaccaaca ttccgctgca
     2881 ccaggagctg ctgctggacg cggcctttct gcgcggcggc accaatatcc actatctcga
     2941 gcagcggctc gccaggctcg cccgggatga gtgattcacg gcaggtgcaa gttacaagtt
     3001 actaggtaca agttacagcg tccaaacgct aagaccttga accttgagtc ctgtaccttg
     3061 taactgtatt catgtcctgg atttcggttt cctttgaagt cgcggcggct gaggtcgagg
     3121 cggtatccga cgcgctgctg gaagcgggcg cggcaagcgt ggacgtggcg gatgccgatg
     3181 ccggatccgc ctccgagcgg ccggtgttcg ccgagcccgg gggcgacgca ccgctcggct
     3241 ggcggcgcag tctcgtcacc gcgcttgccg cggagggcac cgatgccgcg gcgctcgtgg
     3301 ccgccgcctg cgccagggcg ggcatcgcgc cgccgccgca tcgcgcgcgc cgcgtcgccg
     3361 agcaggactg ggtgcgcgcg agccgcgacc agatcacgcc gatccgggtt tcgcagcggc
     3421 tgtggatcgt gccgagctgg cacgcaccgc ccgatcccgg cgcgatcaac ctcctgctcg
     3481 acccgggact cgccttcggc accggcagcc atcccactac gcgtctgtgc cttgagtggc
     3541 tcgagcgcac ggtcagaggc ggagaaaccg tgctcgacta cggctgcggc tccgggatac
     3601 tcgccattgc ggcaatgaag ctcggagcgg cgcgcgcgga cggcgtggac atcgacccgc
     3661 aggcgctgct tgcggcgcgc gcgaatgccc ttcacaatcg ggtgcaggca acttttcacg
     3721 gcgccgccga cgccattcag cagcccgcgc aaatcgtggt cgccaacatc ctcgcccatc
     3781 cgctcatcgt gctcgcgccg ctcatcgcgc ggctcaccgc gcgcggcggg cggctcgcgc
     3841 tcgccggcct gctcgcggga caggaggacg gggtgcgcgc cgcgtacgag ccgtggttcg
     3901 atttcgaggc gcccgcgcgg gacgaaggct ggatgctcct cgccggcgcg aggcgtgccg
     3961 catgagcctc gttacgaagt gcgcgcattg cggcacgacg ttccgcgtca cgcagccgca
     4021 actgcaggcg cataacgggc gggtgcggtg cggcacctgc atgacggtat tcgacgggct
     4081 gaaggagctg gtcacgctgc ccgagcccgc accggaaacc tcaccgaaaa cggcggctgg
     4141 cgcaggagat gtcgccggat tcgaactgga gccggtggtt ccggcagcga taacgaccgc
     4201 gccagccatc ccggtcccgc ccgaagccgt gcatgaggag cgggactacg gcccggtgcc
     4261 ggagcagctc tcgctcgacg atcagctcta tcccgaggag gcgcggcgcc gcgcccgctg
     4321 gtgggcggtg ggcacggctc tgctcgcgtt tgtactcgca ggtcaagcgg tttacttcca
     4381 ccgggcagac ctttccgcgc aatacccggg gctgaagcct tacctggtcc ggttctgcgg
     4441 cctgctgcgc tgcacggtgt cgccgccgca gcggcccaag cagattgcga ttgaagcctc
     4501 cgacctgcag gcgctggacg cggcgcgccc gcagatcatc cagttgaccg ccacgctgcg
     4561 caatcatgcc ggatacgatc tcgccttccc ggtgctcgat ctcgtgctca ccaacaccaa
     4621 ggagcacacg ctcgcgcggc gcctctttac gccggcggag tacctcgagc gcggccgcga
     4681 tgtcatggcg ggcattccgg ccaacgcgga gatcaccgtc cgtctcgacc tcgatgccgg
     4741 ggacctgaac cccgccggct tccggctcga cctgctgccc gccccgcagc cgtaagatca
     4801 atccgccaag acgccaaggc cgccaagaga acacaaataa tgaatctgcc ggaagacctc
     4861 aggtacaccg ccgagcacca gtgggtgcgg atggaggccg atggcacggc ggcggtgggc
     4921 atcacggacc acgcgcagga gcagctgggc gacgtggtgt tcgtgcagtt gcccgaagcc
     4981 ggccgcaagg tgaaacgggg ggaaacctgc gccgtggtcg agtccgtcaa ggcggcgtcc
     5041 gatattcccg cgcccctctc cggcgagatc gtcgcagcca acccggcagc ggccgatgca
     5101 cccgagaaac tcaaccacga accctatgcg gcgtggctgt tccgcatccg gcccggtgtt
     5161 gcagcggagt tgacgggatt gctggatgcc gaggcatata gcaggacagt gatgagtgat
     5221 aagtgatgag tggaaagtag cttgctggcg cgttgcaggc ttgcgcgtgc ctggggtgcg
     5281 gggcatactg cggcgctcga tccggacacc ggaaacacaa aggaggacac gcatggccaa
     5341 cgtcacgctc ggcggcaacc cgatcaccgt cgcgggcgct ctcccgaaga aaggcgatag
     5401 cgctcccgat ttcactctga ccgccaggga cctgaaggac atgggcctcg gggatttcgc
     5461 gggcaagcgc aaagtgctca acatcgtgcc gagcctcgac acgccggtgt gcgcgaagtc
     5521 cacccgggtg ttcaacgaga aagcgggcgc gctgccgaac acggtcgtgc tggtgatttc
     5581 ggccgacttg ccgttcgcgg ccgggcgatt ctgcaccgcg gaggggatcg acaacgtggt
     5641 gacgctctcc acgatgcgcg ggggcgattt caagacgaag tacggcgtgg acatcaccag
     5701 cgggccgtta aaaggcgcga cggcgcgcgc ggtgatcgtg ctcgacgcag gcaacaaggt
     5761 tctgcattcc gagctagtgc cggagatcaa gcaggaaccg gattacgacg cggcgctcgc
     5821 cgtgctgaac tagaaaataa ccgccaagac cgccaagaaa aatgacggac gatccgcgaa
     5881 tcacgaatca cgattcacgg gtcggggaaa gcccgcacaa ggggaagacc ggcttgcggc
     5941 ggatctggaa cgctttcttc tattcgctcg ccggtcttgc cgccgccttc cgacacgagg
     6001 acgccttccg ccaggaagtc ttcctgggcg ccattctgat tcccgccgcg ttcttcgtgc
     6061 cggcgaacgg cgccggaaga gcgctgatgg tcggcgccgt gctgctcgtc ctgatcgtcg
     6121 agctgctcaa ctcggcggt
//
LOCUS       DBNGSG010000464         4462 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3545862, whole genome shotgun sequence.
ACCESSION   DBNGSG010000464 DBNGSG010000000
VERSION     DBNGSG010000464.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4462)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3545862"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="AC158Q_12145"
     CDS             <1..393
                     /locus_tag="AC158Q_12145"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3340569.1"
                     /translation="LRSNQVQVAFEITAPVVGLLKSGAVRALAVSPATRYPGLPDVPT
                     VAEAGVPGYQVTSWNGVAVPARTPRAVINRLNKAVTDAVIAADTRQRFQELGAEARAA
                     TPEEFRAFFAGEITKWAKVIEAAKIPKQ"
     gene            513..1166
                     /locus_tag="AC158Q_12150"
     CDS             513..1166
                     /locus_tag="AC158Q_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3340570.1"
                     /translation="MPQYTLHLYTDQLSPKSTVEMPAATRVVYVREGDAILRAGGQAA
                     RLAANSAWHGRDAVSVAAGAAGATLWRWELAAGALADKLAGAASTLTLAHPVELADPG
                     GYLMRCDRVDFPPGGIAYTHVHRGPGIRCLLAGGIRVEVNGKSHDVAPGEAWFEAGPD
                     PVLALASETAPTAFTRVMILPRELKGKSSIRYVKPEDAGKPKLQTYRVFVDEVIELE"
     gene            1296..1574
                     /locus_tag="AC158Q_12155"
     CDS             1296..1574
                     /locus_tag="AC158Q_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340571.1"
                     /translation="MRDYWLSKLFFDLQSPPLADEYRADRGAVLARYPLKPDVRAAVE
                     ADDVAALAKLVNPYLLRFYFLMAGMPEDDFLRRIRATGAPGMAGAAHG"
     gene            1567..2397
                     /locus_tag="AC158Q_12160"
     CDS             1567..2397
                     /locus_tag="AC158Q_12160"
                     /inference="COORDINATES: protein motif:HMM:NF014899.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DODA-type extradiol aromatic ring-opening family
                     dioxygenase"
                     /protein_id="HIR3340572.1"
                     /translation="MAELVGVYAASHGPMIVRNWEKLAPAERDSLTSAFSELGRRIKA
                     ARPDALIVISPDHWVNFFIDNLPSICVGVGEEHGGPPEPWFKAFPHRKLAGHPALAEH
                     VVRTALEHDFEPSVSYQLALDHGFCIPLWKAGVDPIPAIVPVIFNDIEPPFPSVKRCY
                     AWGAMLADAVASFPGGLRVAVLATGGLSHSIGEHDMGRIDEAFDRDCLRRFEQGDPPA
                     LLEFLNQRLPSAGNGASEVRNWVAAHGVARGRGFELIRYDPIPNVYVGCGFASWKLGG
                     "
     gene            2553..3212
                     /locus_tag="AC158Q_12165"
     CDS             2553..3212
                     /locus_tag="AC158Q_12165"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR00080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HIR3340573.1"
                     /translation="MRRALADAIEQGVNQARSQTGGAPIDKRVIAAMGKVPRHEFAPP
                     ELRPYAYLDRPLTVGPGPDSTISQPSLVAMMTDLLGIKRGEKVLEVGVGGGYQTAILA
                     ELTEQVHSVEFSPKVAEAARQTLKRLGYVGVKVNVADGYYGWRPDGPYDAILVRMAIP
                     DVTAALIEQLKPGGRLIAPVGPDGDVQILVLMRKEAGGGVTMTGIMPVRFRPLPGGTR
                     L"
     gene            complement(3217..3768)
                     /locus_tag="AC158Q_12170"
     CDS             complement(3217..3768)
                     /locus_tag="AC158Q_12170"
                     /EC_number="1.13.11.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HIR3340574.1"
                     /translation="MFDQERFIEDCRAAIRGKDSQNVIRELVARAVSEPAQLMRVIGE
                     PKRAGVETIYKADNLTILNLCWGPGMVFKPHDHHMWAVIGIYTGREENTFFRRAERGL
                     TRHGTKELNAGDVTPLGAAIIHAVTNPLDRITGAIHVYGGDFFAVQRSEWDPRTFEEQ
                     PYDMKDTLRAFEESNARLGFKAT"
     gene            complement(4196..>4462)
                     /locus_tag="AC158Q_12175"
     CDS             complement(4196..>4462)
                     /locus_tag="AC158Q_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HIR3340575.1"
                     /translation="GVSRLARYNITAEALSGGGNKVKYFEGTPIPTSLLLVLLMFWAA
                     TQGALGESLWFGQAVLAGFTLHPLVLLFAVSGSLMISRIRIPKL"
BASE COUNT          766 a         1522 c         1403 g          771 t
ORIGIN      
        1 ctgcgcagca accaggtgca ggtcgcgttc gaaattacgg cgccggtggt cggtctgctc
       61 aagagcggcg cggtgcgcgc gctcgcggtg tcgccagcga cgcgttaccc cgggctgccc
      121 gatgtgccga ccgttgccga ggccggcgtc cccggctacc aggtaacgtc ctggaacggc
      181 gtcgcggtgc cggcgcgcac gcccagggcc gtcatcaacc ggctcaacaa ggccgtcacc
      241 gatgcggtca tcgccgccga cacccggcaa cgcttccagg aactcggcgc cgaggcacgt
      301 gccgcgacgc ccgaggagtt ccgcgccttc ttcgccggcg aaatcaccaa gtgggcgaag
      361 gtgatcgagg cggcgaagat tcccaaacag tgagaagcgt gagtcgtgat tcgtgaatcg
      421 tgattggacc cgaacagcat tgtcttcccc cgttagagga agctaaaaca aggcgcctcg
      481 acccgcgaat cacgaatcac gcctttccag gcatgccgca atacaccctc cacctctata
      541 ccgaccagct ttctccaaag agcacggttg aaatgccggc ggcaacccgc gtcgtctacg
      601 tgcgcgaggg cgatgccatc ttgcgtgccg gcgggcaggc ggcgagactg gccgcgaaca
      661 gcgcgtggca tgggcgtgac gcggtctcgg tcgctgcggg cgcggcgggc gcaacgctgt
      721 ggcggtggga actcgccgcc ggcgccctcg cagacaaact ggcgggcgca gcatccacgc
      781 tcacgcttgc ccacccggtc gagctcgcgg acccgggcgg ctacctgatg cgctgcgacc
      841 gcgtggattt tcccccaggc ggcatcgcct acactcacgt ccaccgcggc ccgggcatcc
      901 gctgcctgct ggcgggtggc atccgcgtcg aggtgaacgg aaaaagtcac gacgtcgcgc
      961 cgggtgaagc gtggttcgag gccggccccg acccggtgct ggcgctcgcg tcggaaaccg
     1021 cgcccaccgc tttcacccgg gtcatgatcc tgccgcggga actgaagggc aagagctcga
     1081 tccgctacgt gaagcccgag gacgccggca agcccaagct gcaaacctac cgcgtttttg
     1141 tcgacgaggt catcgagctt gaatagagcg tgaccggtga cggcacaatt tggcagccgt
     1201 cggcgggcaa taactgcaaa ctcgccacca caaactgcaa acttgtatca ttcatcactg
     1261 ggcacccgac attcgacact cgacactgta tccaaatgcg cgactactgg ctctccaagc
     1321 tgttcttcga cctgcaaagc ccgccgctgg cggatgagta ccgcgccgac cgcggcgcgg
     1381 tgctcgcgcg ctacccgctg aagccggatg tgcgcgcggc ggtcgaggcc gacgacgtcg
     1441 cggcgctcgc gaagctggtg aacccgtacc tgctgcgctt ctatttcctc atggccggca
     1501 tgccggaaga cgatttcctg cgccggatcc gcgccaccgg cgcgcccggc atggcggggg
     1561 ccgcccatgg ctgagctcgt cggcgtctac gcggccagcc acggcccgat gatcgtgcgc
     1621 aactgggaaa agctcgcgcc ggcggaaaga gacagcctca cctcggcgtt ctccgagctc
     1681 ggccgccgca tcaaggcggc gcggcccgac gccctgatcg tgatctcgcc ggatcactgg
     1741 gtgaatttct tcatcgacaa cctgccgtcg atctgcgtgg gcgtgggcga agagcacggc
     1801 gggccgcccg agccgtggtt caaggccttc ccgcaccgca agctcgcggg gcacccggcg
     1861 ctcgcggaac acgtcgtgcg caccgcgctc gagcatgatt tcgagccgtc ggtctcgtac
     1921 cagctcgcgc tcgatcacgg cttctgcatc ccgctgtgga aagcgggtgt ggaccccatc
     1981 cccgccatcg tcccggtgat cttcaacgac atcgagccgc catttccctc ggtgaagcgc
     2041 tgctacgcct ggggcgccat gctcgcggac gcggtcgcaa gtttccccgg cggcctgcgc
     2101 gtcgcggtgc tggcgaccgg tggactcagc cactcgatcg gagaacacga catgggccgg
     2161 atcgacgagg ccttcgaccg cgactgcctg cgccgcttcg agcaaggaga cccccccgcc
     2221 ctcctcgaat tcctcaacca gcgcctgccc tccgccggca acggcgcctc cgaagtccgc
     2281 aactgggtgg ccgcccatgg cgtggcgcgc gggcgcgggt tcgagctgat ccgctacgac
     2341 ccgataccga acgtgtacgt cggctgcggc ttcgcctcct ggaagctcgg gggatgagcc
     2401 acaaggaaaa aggcaaaagg aaaaaggcaa gaggattcgg ggtttccttt cgccttccga
     2461 ctttcgcctt tcgacttgct ggtcccgcgt tcctcctatg ctgcgcactg gcgggaacag
     2521 ccggcgcgca ggaagcgcag gagttcgcgg agatgcgacg cgccctcgcc gatgcgatcg
     2581 agcagggcgt gaatcaggcg cgcagccaga ccggcggggc gccgatcgac aagcgcgtga
     2641 tcgcggccat gggcaaggtg ccgcgccacg aattcgcacc gccggagctg cgaccctacg
     2701 cgtatctcga ccggccgctc acggtcgggc ccggcccgga ttcgacgatc tcgcagccgt
     2761 cgctggtcgc gatgatgacc gacctgctcg gaatcaagcg cggggagaaa gtgctcgaag
     2821 tcggcgtcgg cggcggctac cagaccgcga tcctcgcgga actgacggaa caggtgcaca
     2881 gcgtcgagtt cagccccaag gtcgccgagg ccgcgaggca gaccctcaag cggctcggct
     2941 acgtcggcgt gaaggtcaac gtcgccgacg gctattacgg ctggcgcccg gacggcccgt
     3001 acgacgccat ccttgtccgc atggcgatac ccgacgtgac cgccgcgctg atcgagcagt
     3061 tgaagccggg cgggcggctc atcgcgccgg tcggcccgga cggggacgtg cagatactgg
     3121 tactgatgcg caaggaagcc ggcggcggcg tgaccatgac cggcatcatg ccggtgcgat
     3181 ttcgcccgct gccgggtgga accaggttgt gatgctttac gtcgctttga aaccgagacg
     3241 cgcgttcgat tcttcaaacg cccgcagcgt gtccttcatg tcgtagggct gctcctcgaa
     3301 cgtcctcggg tcccattcgc tgcgctgcac ggcaaaaaag tctcccccat acacgtggat
     3361 ggccccggtt atccggtcga gcggattggt caccgcgtgg atgatcgccg cgccgagagg
     3421 ggtcacgtct cccgcgttca gttccttggt tccatgccgc gtcaggcccc gttccgcccg
     3481 gcgaaagaag gtgttctcct cccggccggt gtagatgccg atgaccgccc acatgtgatg
     3541 atcgtgcggc ttgaatacca tgcccggccc ccagcacagg ttgaggatcg tcaggttgtc
     3601 cgccttgtag attgtctcga cccctgcgcg cttcggctcg ccaatcaccc gcatcagttg
     3661 cgcgggctcg ctgacagccc tggccaccag ctcccggatc acgttctgcg agtcctttcc
     3721 cctgatggcc gcgcggcaat cctcgatgaa gcgctcctgg tcgaacatgg ccaacccccc
     3781 gttttcgatg cggtgtgact cgcgactcga gattcgaccc tctccgcttc cgccttaggc
     3841 cttccccatc attcagcacg gtcgtatcaa gcgcattttg cgccgccagg tgtgcgtctt
     3901 caagccggac tttacccctc accccaaccc tctccccgct gatgcagggc gagggcgtgt
     3961 tttgagaggc acattattct cagccggtaa cgcaaccagg agtatttcct agaggccacc
     4021 cttggcgcgg ttccttcgag accttgggtt gtttttatta ttgaatcctt cgcgcccttt
     4081 gcggccttag cgacacgtcg tacgctttcc ttggcgtgct tggcgtcccc ttcgactcac
     4141 tcaggacaag cttggcggtg aagctttacg tcgtttctag aactgcatgc gcaactcaga
     4201 gcttcgggat ccgtatgcga ctgatcatca gcgagccgga aaccgcaaaa agcagcacca
     4261 acggatgcag cgtgaagccc gcaagcacgg cctgtccgaa ccagagggat tcgcccaatg
     4321 caccctgcgt ggcggcccag aacatcagca acaccagcag cagggacgtc ggaatcggcg
     4381 ttccctcgaa gtacttcacc ttgtttccgc cgccggacag cgcctcggcc gtgatgttgt
     4441 agcgcgccag gcgtgacact cc
//
LOCUS       DBNGSG010000465         2153 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1508916, whole genome shotgun sequence.
ACCESSION   DBNGSG010000465 DBNGSG010000000
VERSION     DBNGSG010000465.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1508916"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /gene="phnE"
                     /locus_tag="AC158Q_12180"
     CDS             complement(<1..754)
                     /gene="phnE"
                     /locus_tag="AC158Q_12180"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HIR3340576.1"
                     /translation="MNVSAATAARAWRAPPLIGNPWLRWGIYFGALLYLVLAIGSLQI
                     NWARVAEGLERGWRFISAFAQPNFAGRWGEISEGILESITMAVASTVIGIALAVPVGL
                     GAARNLAPLPLYMACRAIIALSRTFQEVIIAILFVKMFGFGPLAGMVTLVVATVGFMA
                     KLLAEGIEGADPAQMEAIRATGGSWWQLINYGVQPQVMPRFIGLSVYRLDINFRESAV
                     IGIVGAGGIGATLNTAIDRYEFDSAAAILLIII"
     gene            complement(751..1605)
                     /gene="phnC"
                     /locus_tag="AC158Q_12185"
     CDS             complement(751..1605)
                     /gene="phnC"
                     /locus_tag="AC158Q_12185"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018573518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HIR3340577.1"
                     /translation="MSGLPGPMLALRGLTKRYPGGDLAVSNVSLEVPRGQVMALIGPS
                     GAGKSTLIRCVNRLVEPTAGQVMLDGDDLTVLGGAPLRRARRRMGMIFQEFALVERLT
                     VMENVLSGRLGYVGFWRSFLRRYPRDDIDRAFRFLDRVGLSEFVDKRADALSGGQRQR
                     VGIARALMQEPAILLVDEPTASLDPKTSRQIMRLICEICAERGLAAIINIHDVALAQM
                     FAARVVGLCKGRIVFDGAPRELTQDALTLIYGEEDWKSTIRRDDDEDGPQAAVDGNGG
                     RGSGPAAA"
     gene            complement(1602..>2153)
                     /gene="phnD"
                     /locus_tag="AC158Q_12190"
     CDS             complement(1602..>2153)
                     /gene="phnD"
                     /locus_tag="AC158Q_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR3340578.1"
                     /translation="SFGYEMEIIVHADSPIRKIEDIRGRTLAFTDPTSSSGFKAPSAL
                     LRSEFRLTPDKDFKTAFSGKHDNSVIGVVNKDYDAAAVANSVMKRMLARKVVDGAKIR
                     TIYKSQTFPTTAYGYAYNLQPELAKKVREAFLTFDWEGTELKKEFGKGSPPREKFFPI
                     TYKEQWDVVRKVDQAMGVGYACK"
BASE COUNT          395 a          716 c          681 g          361 t
ORIGIN      
        1 cgatgatgat gagcaggatg gccgcagccg aatcgaattc gtagcggtcg atcgcggtgt
       61 tgagggtcgc accgatgccg ccggcgccga cgatgccgat caccgcggat tcccggaaat
      121 tgatatcgag ccggtacacc gacaggccga tgaagcgcgg catcacttgc ggctgcactc
      181 cgtaattgat gagctgccac cacgacccgc cggtggcgcg aatcgcttcc atctgggcgg
      241 gatcggcgcc ctcgatgccc tcggccagca acttcgccat gaaaccgacc gtggcgacga
      301 ccagcgtcac catgcccgcg agcgggccga aaccgaacat cttcacgaac aggatcgcga
      361 tgatcacttc ctggaacgtg cgcgatagcg caatgatggc gcgacacgcc atatagagcg
      421 gcaaaggggc gagattgcgt gccgccccca gccctaccgg aacggcaagc gcgatgccga
      481 tcacggtaga cgcgaccgcc atcgtgatgc tctcgaggat gccctcgctg atttcccccc
      541 accgccccgc gaagttgggc tgggcgaagg cggaaatgaa gcgccaaccg cgctcgaggc
      601 cctcggcgac ccgcgcccag ttgatctgta gcgagccaat ggccagcacc aggtagagca
      661 acgcgccgaa gtagatgccc cagcgcagcc atggattgcc gatcagcggc ggcgcgcgcc
      721 aggcgcgggc ggcggtcgcg gcggaaacgt tcacgcggct gccggtccgc tcccgcggcc
      781 gccgttccca tccacggctg cctgcgggcc gtcctcgtcg tcatcgcggc ggatggtgga
      841 cttccagtcc tcctcgccgt aaatcagggt cagcgcgtcc tgcgtcagct cccgcggtgc
      901 gccgtcgaac acgatccttc ccttgcacag tccaacgacg cgtgccgcga acatctgggc
      961 gagcgccacg tcgtggatgt ttatgatggc ggcaagcccg cgttctgcac agatctcgca
     1021 gatgagccgc atgatctgcc gcgaggtctt gggatcgagg ctggcggtcg gctcgtccac
     1081 cagcaggatg gccggttcct gcatcagcgc ccgggcgatg cccacgcgct ggcgctgccc
     1141 gcccgacagc gcatcggcgc gcttgtccac gaattcggaa agcccgaccc ggtcaaggaa
     1201 acgaaacgcc cggtcgatgt cgtcccgcgg ataacggcgc aagaaactcc gccagaaccc
     1261 cacgtaaccg aggcgcccgg agagcacgtt ctccatcacg gtgagccgct cgaccagggc
     1321 gaattcctgg aagatcatgc ccatgcgccg gcgcgcgcgg cgcagcggcg caccgcccag
     1381 cacggtaagg tcatcgccat cgagcatgac ctggccggca gtgggctcaa ccaggcggtt
     1441 gacgcagcgg ataagcgtgc tcttgcccgc gcccgacggc ccgatcaggg ccatcacctg
     1501 ccctctgggc acttcgaggc tgacgttact gaccgcgaga tcgccgcccg ggtagcgctt
     1561 ggtgagcccg cgcagcgcaa gcatggggcc cggcaagccg ctcatttaca ggcgtagccg
     1621 acacccattg cctggtcaac cttgcgcacg acgtcccatt gctccttgta ggtaatcggg
     1681 aagaatttct cccgcggcgg gctgcccttg ccgaactcct tcttcaattc ggtgccctcc
     1741 caatcgaaag tgagaaaggc ttcgcgcacc ttcttcgcca gctcgggctg caggttgtag
     1801 gcgtaaccgt aagcggtcgt cgggaaagtc tgggacttgt aaatggtgcg tattttcgcg
     1861 ccgtccacca ccttgcgcgc gagcatgcgc ttcatcacgg aattcgcaac cgcggcggca
     1921 tcgtagtcct tgttcaccac gccgatgacg gagttgtcgt gcttgccgga aaacgcggtc
     1981 ttgaaatcct tgtcgggtgt gagcctgaat tctgagcgaa gcagcgccga gggcgccttg
     2041 aaaccggagc tcgacgtagg gtcggtaaag gcgagcgtgc ggccgcgtat gtcttcgatc
     2101 ttccggatcg ggctgtcggc atgcacgatg atctccatct cgtagccgaa cga
//
LOCUS       DBNGSG010000466         3433 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1338719, whole genome shotgun sequence.
ACCESSION   DBNGSG010000466 DBNGSG010000000
VERSION     DBNGSG010000466.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1338719"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..1021)
                     /locus_tag="AC158Q_12195"
     CDS             complement(44..1021)
                     /locus_tag="AC158Q_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3340579.1"
                     /translation="MERMKRLFSAGAGCAFVVFSGALVAQGYPAKPVRVVVPYAPGGA
                     TDLTARLVGQKMQAAMKQNVLVDNRPGAGGVIGADIVAKAAPDGYTVLIAVPAEMAIL
                     PHLQKMPYSVPRDFAPVSLATVTPLIFVVHPSLPVQSVKELIAFVKARPGQLSYASAG
                     TGGVQHLSGELLKITMKLDMAHVPYKGAGPVMPDLIGGHVPMFFSGMPPAMPHVKAGK
                     LRALAVTTTRRSPAAPEVPTMTEAGVPGFDISNWFAYFVPAGTAPEVISRLNAEVNRG
                     LKFQDVKEKLANVGAETVGTTPGELAKFVRDETEKFARLVKLSGAKGTD"
     gene            complement(1038..2015)
                     /locus_tag="AC158Q_12200"
     CDS             complement(1038..2015)
                     /locus_tag="AC158Q_12200"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3340580.1"
                     /translation="MNGKCVVVVLAALGASAITQPVSAQEYPSRPLRMLVAFAPGGNT
                     DILARAVGARMAENWGRPVVVDNRPGGSGMLAAEIVAKAAPDGHTVFVGSTGELSINP
                     SLFRKMPYNVQRDFAPVTLGTVSPLLVVVHPSFPAKTLAELLKIARARPGTLAYASVG
                     IGTPQHLSGELFKMVTGTDIVHVPYKGGAPATTALLGGQEVQFGFVGMGPVIPHVQSG
                     RLRALAISTARRSNLLPEVATLQELGVKDFDTSIWFAFVVPAKTPKGIIGRLNAEIVR
                     ILRRPDVRDYLLGTGVEVTPGAPDELARFIKSEGEKYRKIIQVSGSSQN"
     gene            complement(2072..>3433)
                     /locus_tag="AC158Q_12205"
     CDS             complement(2072..>3433)
                     /locus_tag="AC158Q_12205"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase family protein"
                     /protein_id="HIR3340581.1"
                     /translation="IDALAARGTMFSSAYTACPVCVPARAAFATGKYVHQIGFWDNAD
                     AYDGSIPSWHHRLREAGHHVASIGKLHFRGHEGDDYGFSEVQIAMQIIEGKGDLMGLV
                     RNDLPERGMSWKIAKLAGPGETPYTAYDRDIATRAQIWLREEAPRHRDKPWVLFVSFV
                     CPHFPLVAPADFYYQYPLDRMPLPKQYAKDERPDHPYLRDYAGSFVYDRYFDPEKLRK
                     AVAAYYGLCSFVDDNVGRVVRTLEAAGLADSTRILYTSDHGDNLGARGLWGKSTMYEE
                     VAGVPLIIAGPDVPKGKRVGTPASHVDTYPFILECAGEDRAQMYDGHPGVSLTRLAHG
                     EQPQRNVLSEYHGMGSTTGAFMIRHGRHKYVHYVAYRPQLFDLEQDPEELRDLAGEPA
                     YAGLVEECRARLFAMCDPAQVDARAKQRQAELLQAAGGREAVIRRGDLGFSPPPGYPA
                     VFG"
BASE COUNT          535 a         1203 c         1086 g          609 t
ORIGIN      
        1 cgctccgtca cccgtcactc gtccctcgtc acccgatact cggctagtcc gtgcccttcg
       61 cgccggacag cttgaccagc cgcgcgaatt tctcggtctc atcacgcacg aacttcgcca
      121 gctccccggg tgtcgttccc accgtctccg cgcccacgtt ggcgagcttc tctttcacgt
      181 cctggaactt gagtccgcgg ttgacctcgg cgttgagccg ggagatcacc tccggcgccg
      241 ttcccgccgg cacgaaatac gcgaaccagt tgctgatatc gaagcccggc acgcccgcct
      301 ccgtcatggt gggtacttcg ggagccgcgg gcgagcgccg ggtcgtcgtc accgccagcg
      361 cgcgcagctt ccccgctttc acgtgcggca tggcgggcgg catgcccgag aagaacatcg
      421 gcacgtgacc gccgatcagg tccggcatca cgggccccgc gcccttgtac ggcacgtgcg
      481 ccatgtcgag cttcatcgtg atcttgagca gctcgcccga cagatgctgc acgccgccgg
      541 tgccggccga cgcgtagctc agctgccccg gccgcgcctt gacgaaggcg atcagttcct
      601 tcaccgactg gaccggcagc gagggatgca cgacaaatat gagcggcgtc accgtcgcga
      661 gcgacaccgg cgcgaagtcc ctggggacgc tgtacggcat tttttgcagg tgcggcagta
      721 tcgccatttc cgcgggcacc gcgatcagca cggtgtagcc gtccggcgcc gccttcgcca
      781 cgatgtcggc gccgatcacg ccgcctgcgc cgggacggtt gtccaccagc acgttctgct
      841 tcatggccgc ctgcatcttc tgtccaacca ggcgcgcggt caggtccgtc gcgccgcccg
      901 gcgcgtacgg caccacgacc cggaccggtt tcgccgggta accctgcgcc acgagcgcgc
      961 cgctgaagac gacgaatgcg catccggcgc cggccgagaa caagcgtttc atgcgttcca
     1021 tgtggtctct ccccttctca attttgactc gagccggata cctggatgat cttgcggtac
     1081 ttttcaccct ccgacttgat gaagcgcgcc agctcgtcgg gcgcgcccgg cgtgacttcc
     1141 acgccggtgc cgagcaggta atcgcgcacg tccggcctgc gcaggatgcg cacaatctcg
     1201 gcgttgagcc ggccgatgat gcctttcggc gttttcgccg gcaccacgaa ggcgaaccag
     1261 atactggtgt cgaagtcctt cacgcccagt tcctgaagcg ttgccacctc cggcagcagg
     1321 ttcgagcgcc tggcggtcga gatcgccagt gctctcagcc tgccggactg gacgtgcggg
     1381 atgaccggcc ccatgccgac gaatccgaac tgcacctcct gcccgcccag cagggcggtg
     1441 gtcgccggcg cgccgccctt gtacggcacg tgcacgatgt cggtcccggt taccatcttg
     1501 aatagttcgc cggacagatg ttgcggcgtg ccgatgccga ccgacgcgta cgccagtgtg
     1561 cccggcctgg cgcgggcgat tttcagcaac tcagcgagcg tcttcgccgg gaacgaggga
     1621 tggacgacga ccagcagcgg cgacacggtg ccgagcgtga ccggggcaaa gtcgcgctgc
     1681 acgttgtacg gcattttcct gaacaggctc ggattgattg acagctcgcc ggtcgagccg
     1741 acgaagacgg tgtggccgtc gggcgccgct ttggccacga tttccgccgc cagcatgccg
     1801 ctgccgccgg gccggttgtc cacgaccacg ggcctgcccc agttctcggc catgcgcgcg
     1861 cccaccgcgc gcgcgaggat atcggtgttg ccgccgggcg cgaacgcgac cagcatccgt
     1921 agcggccgtg acggatattc ctgcgccgac accggctgcg tgatggctga cgccccaagc
     1981 gccgcgagca ccacaacaac gcattttccg ttcatgtttt cctccccttc aggacatctt
     2041 ccattttcgc ggtgcctcgc gccttgtgcc ttcaaccaaa caccgccggg taccccggcg
     2101 gcgggctgaa acccaggtcg ccgcgccgga tcaccgcctc gcgaccgccg gccgcctgca
     2161 acagttccgc ctgccgctgc ttggcgcgcg catccacttg cgccggatcg cacatcgcga
     2221 acaatctcgc acggcactcc tcgaccaatc ccgcgtatgc cggttcgccg gcgagatcac
     2281 gcagttcctc cggatcctgc tccaggtcga agagctgggg acggtacgcg acgtagtgaa
     2341 catacttgtg ccttccgtgc cggatcatga atgcgccggt ggtcgagccc atgccgtggt
     2401 actccgacag cacgttgcgc tgcggctgct cgccgtgcgc gagcctggtg agcgataccc
     2461 cgggatggcc gtcgtacatc tgcgcgcggt cctcgcctgc gcattccagg atgaacggat
     2521 aggtgtccac gtggcttgcc ggggtgccca cgcgcttgcc cttcggcaca tcgggtccgg
     2581 cgatgatcaa gggcacgccc gcgacttcct cgtacatcgt ggatttgccc cataacccgc
     2641 gcgcgccgag gttgtcgccg tgatcgctcg tgtacaggat gcgcgtggaa tcggcgaggc
     2701 cggccgcctc cagcgttcgg accaccctgc cgacgttgtc gtccacgaaa ctgcagagcc
     2761 cgtagtacgc cgcgaccgcc ttgcgcagct tctccggatc gaagtagcgg tcgtagacga
     2821 agctgccggc ataatcgcgc aggtacggat ggtcgggccg ctcgtccttc gcatactgct
     2881 tgggcagcgg catccggtcg agcgggtact ggtagtagaa atcggccggc gccaccagcg
     2941 gaaaatgagg gcagacgaag gaaacgaaca acacccaggg cttgtcgcgg tgcctgggcg
     3001 cttcctcgcg cagccagatc tgcgcgcgcg tggcgatatc ccgatcgtac gcggtatacg
     3061 gcgtctcccc ggggccggcg agcttggcga tcttccacga catgccccgt tccggcaggt
     3121 cgttgcgcac gagccccatc aggtcgccct tgccctcgat gatctgcatg gcaatctgga
     3181 cttcactgaa gccgtagtcg tcgccttcat ggccgcggaa atgaagcttg ccgatggagg
     3241 cgacgtggtg gcccgcttcg cgcagccggt ggtgccagct cgggatcgag ccgtcgtagg
     3301 catcggcgtt gtcccagaaa ccgatctggt gcacgtattt tccggtcgcg aacgccgccc
     3361 gcgcgggcac gcacaccggg caggcggtgt aggcggacga gaacatcgtc ccgcgcgcgg
     3421 ccagcgcgtc gat
//
LOCUS       DBNGSG010000467         3680 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1678623, whole genome shotgun sequence.
ACCESSION   DBNGSG010000467 DBNGSG010000000
VERSION     DBNGSG010000467.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1678623"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="AC158Q_12210"
     CDS             <1..251
                     /locus_tag="AC158Q_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HIR3340582.1"
                     /translation="SVTQAFCRDCTRIRLSTEGKLYTCLFATAGHDLRILLRNGTTDS
                     EIHDAVAAIWAKRADRYSEIRTSQTEKLEKIEMSYIGG"
     gene            301..1152
                     /locus_tag="AC158Q_12215"
     CDS             301..1152
                     /locus_tag="AC158Q_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HIR3340583.1"
                     /translation="MKTSYRPRITNASRPLTFEVEALNERGEALPTPIAGENPLTLYV
                     DKREIVTLMTLGQAPEALAIGYLRNQRLARSIEEIAEVQVDWETDSVAITTRKPTAAA
                     AKKMKDKMKKRTVTTGCGQGTVFGDLMEEIDSLKLRDDVRLSEETLYALLDAVRRHET
                     IYKSAGAVHGCALASAGAEILNFVEDVGRHNAVDAIAGQMWLDRVDGSDKIFYTTGRL
                     TSEMVIKAAQMQIPFLVSRSGLTQMGFEIANRVGITMIGRAVNKHYLLFTGKHRFVKT
                     VMPTMLA"
     gene            1254..1838
                     /gene="mobA"
                     /locus_tag="AC158Q_12220"
     CDS             1254..1838
                     /gene="mobA"
                     /locus_tag="AC158Q_12220"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HIR3340584.1"
                     /translation="MITGVILAGGRGTRMGSVDKGLKILRGKPMVQWVAERFGAQVDE
                     LLINANQNIDTYAGLGYRVIPDEIAGFAGPLAGLQRGLAEAAHELVATAPCDSPFLPL
                     DLIARLRGALDRENAELAVARTGDQPHPVFCLCRRRVLPGLTDFLAGGGRKIDAWYAR
                     LNTVEVPFDDQPDAFSNVNTEAELKALEGGPLPG"
     gene            1895..2599
                     /locus_tag="AC158Q_12225"
     CDS             1895..2599
                     /locus_tag="AC158Q_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HIR3340585.1"
                     /translation="MERREFIKACAATCAIGASDTLSAKDLKPRFYSRAQLIDERGQP
                     LRAAELVAGRNYIFHYPFASTPCFLLNLGKPTVRDVQLKTEDGSSYLWPGGIGPNRAI
                     VGFSAICAHRMSYPTPQISFISYRDKSTASAAMRPNVIHCCSEHSEYDPSSGARVVGG
                     PAPQPLSAILLEYDAGNGRLFAVGTLGGEMYDAFFDKFGFKLALEHGAGSVRRQVSGT
                     AVVKDLQQYCKQQVRC"
     gene            complement(2630..>3680)
                     /gene="glk"
                     /locus_tag="AC158Q_12230"
     CDS             complement(2630..>3680)
                     /gene="glk"
                     /locus_tag="AC158Q_12230"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HIR3340586.1"
                     /translation="RSGRPRCSHEGPPGVKRGRLLVADVGGTWTTIALAPAGADPVID
                     FHHVYPSQNYDGLEAIVTEFLARPEVARHGGRIEAACFSVAGPVADNASTLTNLGWEV
                     DGAALAQTLGLPAVKLVNDFSAAGQGIGQLAASDLETLQTGEPIERGARLIIGAGTGL
                     GVGVLTWQAGGYAVHPSEAGHCDFAPVDRQQDLLLAHLRRSFGRVSYERVVSGPGLMR
                     IFSFLQESGDGAPSRQLLEATRKRSDTAELIAEFAISRLDPVAVKALDLFVEVYGAFA
                     GNMALVTLARGGVYIAGGIAPKIAAKLRDGVFMRAFTSKGRFSELLASIPVHVVKNPR
                     VGLLGAIAEAGRQRK"
BASE COUNT          687 a         1242 c         1150 g          601 t
ORIGIN      
        1 cttccgtcac ccaggcgttc tgccgcgact gcacgcgcat ccggctctcc accgagggca
       61 agctctacac ttgtctcttc gcgaccgccg gccacgacct gcggatcctg ctgcgcaacg
      121 gcaccaccga cagcgagatt cacgacgcag tggcggcgat ctgggcgaag cgcgccgacc
      181 ggtactcgga aatccgcacc agccagaccg aaaagctcga aaagatcgag atgtcctaca
      241 taggcgggta actgcgttcg aacggacgca cacgacgcgc cgttcaacgc gttacaaccg
      301 atgaagacaa gctatcgccc cagaatcact aacgccagcc ggccgctgac attcgaggtc
      361 gaagcgctga acgagcgcgg cgaggcgctg cccacgccga tcgccggtga gaatccgctg
      421 acgctctacg tcgacaagcg cgaaatcgtg accctgatga cgctgggcca ggcaccggaa
      481 gcgctcgcca tcggctacct gcgcaaccag cgcctggcgc gctcgatcga ggaaatcgcc
      541 gaggtgcagg tggactggga gaccgactcg gtcgcgatca ccacgcgcaa gcccaccgcc
      601 gccgccgcga agaagatgaa ggacaagatg aagaagcgca cggttaccac cggctgcggc
      661 cagggcaccg tattcggcga cctgatggag gaaatcgact ccctcaagct gcgcgacgat
      721 gtgcggctct ccgaggagac gctctacgcg ctgctcgacg cggtgcgccg ccacgagacg
      781 atctacaaga gcgccggtgc ggtgcacggc tgcgcgctcg ccagcgccgg cgcggagatc
      841 ctcaacttcg tcgaagacgt gggccgccat aacgccgtgg atgccatcgc gggccaaatg
      901 tggctggacc gcgtggatgg ctccgacaag atcttttaca ccaccggccg cctcacttcc
      961 gagatggtga tcaaggcggc gcaaatgcaa atcccgtttc tcgtgtcgcg ctcgggcttg
     1021 acgcagatgg gattcgagat cgcgaacagg gtcggcatca ccatgatcgg ccgcgccgtg
     1081 aacaagcact acctgctatt caccggcaag caccgcttcg tcaaaacagt catgccaacc
     1141 atgctggcat gactgttttt gacgagtgtt gaatgttgaa ttttgagtgt tgaatttcca
     1201 agagtggttg cagtttggct tctcattcaa aactcaaaac tcaaaactca acattgatca
     1261 ccggggtgat cctggccggc ggccgcggca cgcgcatggg aagcgtggac aagggcctga
     1321 agatcctgcg cggcaagccg atggtgcagt gggtggccga gcgcttcggg gcgcaggtcg
     1381 atgagctcct catcaatgcc aaccagaaca tcgacaccta cgcgggcctg ggctatcgcg
     1441 tcattccgga tgaaattgcg ggttttgccg gaccgctcgc gggcctgcag cggggcctcg
     1501 ccgaggcggc gcatgagctc gtcgcaaccg ctccgtgtga ttcgccattt ctgccgctcg
     1561 acctgattgc ccggctgcgg ggcgcgctcg accgcgaaaa tgcggaactc gccgtcgcga
     1621 ggaccggcga ccagccccac cccgtgttct gcctgtgccg caggcgggtg ctgcccgggc
     1681 tcaccgattt tctggcgggc ggcggccgca agatcgatgc ctggtatgcg cgcctgaaca
     1741 ccgtcgaagt ccccttcgac gatcagcccg acgccttcag caacgtcaac accgaggccg
     1801 aactgaaggc gttggagggc ggcccgctgc cgggttgaca ccgcgcacgc cgattctgaa
     1861 caataggcat atcgcttcgc aggagctccg cgccatggaa cgacgggaat tcatcaaagc
     1921 gtgcgcggcg acctgtgcga tcggcgcgtc ggacacgctg tccgcgaaag acctgaagcc
     1981 gcgcttctat tcccgcgcgc agctgattga cgaacgcggg caacctctgc gcgcggccga
     2041 actcgtcgcc gggcgcaact acatctttca ttaccctttc gcatccacgc cgtgcttcct
     2101 gctcaacctg ggcaagccca cggtgcgcga cgtgcagttg aagaccgagg acggctcgag
     2161 ctatctgtgg cccggcggca tcggccctaa ccgcgccatc gtcggcttct cggcgatctg
     2221 cgcccatcgc atgagctacc ctaccccgca gatcagcttc atcagttacc gcgacaaatc
     2281 cacggcctcg gctgccatgc gccccaacgt catccattgc tgctcggaac acagcgagta
     2341 cgacccgtcg tcgggcgccc gcgtggtggg cggcccggcg ccgcagccgc tgtcggcgat
     2401 cctgctcgaa tacgacgccg ggaatggccg cctgttcgcg gtcggcaccc tgggaggtga
     2461 aatgtacgac gcgttcttcg acaagttcgg gttcaagctt gccctcgagc acggtgccgg
     2521 cagcgtgcgg cggcaggtat caggcacggc tgtagtcaag gacctgcagc aatactgcaa
     2581 acagcaggtc cgctgctgag cggcagccgg gattcactgc gcccgggcat cacttgcgct
     2641 ggcggccggc ttccgcgatt gcgccaagga gcccgactcg ggggtttttc accacgtgca
     2701 ccgggatgct ggcgagcaat tcgctgaacc gccccttgct ggtgaaggcg cgcatgaaaa
     2761 cgccgtccct cagcttggcc gcgatcttcg gcgcgatgcc gcccgcaatg tagactccgc
     2821 cgcgcgccag cgtgacgagc gccatgttgc cggcgaaagc cccgtagacc tcgacgaaca
     2881 ggtcgagcgc cttcaccgcg accgggtcga ggcgggaaat agcgaattcc gcgatcagtt
     2941 ccgccgtgtc gctgcgtttc cgggtggcct ccagcagttg ccgcgaaggc gcgccatcgc
     3001 cgctttcctg caggaagctg aagatgcgca tcaacccggg accagaaacc acgcgctcgt
     3061 acgacacgcg cccgaatgag cggcgcaagt gcgcgagcag cagatcctgc tgcctgtcca
     3121 ccggcgcgaa atcacaatga cccgcctcgg acggatgcac ggcgtagcct cccgcctgcc
     3181 aggtaagcac acccacgccc agcccggtcc cggccccgat gatgaggcgc gcgccgcgct
     3241 caatgggctc tcccgtctgc agcgtttcga ggtccgacgc ggcaagttgc ccgatgccct
     3301 gccccgccgc cgagaagtca ttgacgagct tgactgcggg caggcccagt gtctgcgcca
     3361 gcgccgcgcc atccacttcc caccccaggt tggtcagcgt ggaagcgttg tccgccaccg
     3421 gtccggccac cgagaaacaa gcggcctcga tgcggccgcc gtgcctggcc acttcgggcc
     3481 gggcaagaaa ctcggtgacg atggcttcga ggccgtcgta gttctggctc ggataaacgt
     3541 ggtggaagtc gatgaccgga tcggctccgg ccggggcaag agcgatcgtt gtccaggtgc
     3601 cgccgacatc ggctacgagc agtcgccccc gtttcacgcc cggcggcccc tcatgcgaac
     3661 agcgcggtct gccgctgcgc
//
LOCUS       DBNGSG010000468        14015 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4395589, whole genome shotgun sequence.
ACCESSION   DBNGSG010000468 DBNGSG010000000
VERSION     DBNGSG010000468.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 14015)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14015)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14015
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4395589"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..438
                     /gene="rplW"
                     /locus_tag="AC158Q_12235"
     CDS             94..438
                     /gene="rplW"
                     /locus_tag="AC158Q_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HIR3340587.1"
                     /translation="MTTATSNTRQQQRLMQVILAPVISEKSTYVADKHNQVVFRVAQN
                     ATKPEIKAAVEFMFSTKDKKLEVESVKVVNVRGKEKRFGQTIGRRSHWKKAYVSLKAG
                     QEINFAEQAEAK"
     gene            438..1274
                     /gene="rplB"
                     /locus_tag="AC158Q_12240"
     CDS             438..1274
                     /gene="rplB"
                     /locus_tag="AC158Q_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HIR3340588.1"
                     /translation="MLVKMKPVTPGLRQQVRVVNPELHKGGPVPHLVERKNKRGGRNV
                     HGEITMRHQGGGHKQLYRKVDFKRDKDGITAKVERLEYDPNRSAFLALLCYADGERRY
                     IVAPKGVSAGMQVISGAEAPIKNGNTLPLRNIPVGTTIHCIEMLPGKGAQLARAAGAS
                     VQLLAREGDYAQIRLRSGEIRKVHINCRATIGEVGNEEHSLESIGKAGRVRWRGIRPT
                     VRGVAQNPVDHPHGGGEGKKAAARDPVSPWGTLSKGYKTRKNKRTDVMIVRRRNAKAV
                     GG"
     gene            1278..1529
                     /gene="rpsS"
                     /locus_tag="AC158Q_12245"
                     /pseudo
     CDS             1278..1529
                     /gene="rpsS"
                     /locus_tag="AC158Q_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440478.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
     gene            1668..2006
                     /gene="rplV"
                     /locus_tag="AC158Q_12250"
     CDS             1668..2006
                     /gene="rplV"
                     /locus_tag="AC158Q_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HIR3340589.1"
                     /translation="MNTTAKLNGVRLSAQKGRLVADLIRGKPVEQALNILQFAPQKGA
                     VIIRKVLESAIANAEHNDGADIDELKVTHICVERGTFLRRFRAQSKGRGAAIMKPTCN
                     VIVMVGDGRK"
     gene            2009..2812
                     /gene="rpsC"
                     /locus_tag="AC158Q_12255"
     CDS             2009..2812
                     /gene="rpsC"
                     /locus_tag="AC158Q_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HIR3340590.1"
                     /translation="MGQKINPIGFRLALNRNWSSRWYANSKNFPGMLLEDLRVREHLK
                     KKLAHAAVSRVVIERPAKNARITIYSARPGVVIGKKGEDIESLKGELQKMLGVPVHVN
                     IEEVRKPETDAQLIADSISQQLQKRIMFRRAMKRAITNAMRLGAQGIKIASSGRLNGI
                     EIARYEWYREGRVPLHTLRAEIDYGVSEAKTSYGVIGIKVWVFKGEVMQKHEQPAAAV
                     AAQLASAAKPEAAPEAPAGDKPKKPRTPRKPGAKPGTRSGAKNAATRKA"
     gene            2790..3206
                     /gene="rplP"
                     /locus_tag="AC158Q_12260"
     CDS             2790..3206
                     /gene="rplP"
                     /locus_tag="AC158Q_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011901907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HIR3340591.1"
                     /translation="MLQPARRKYRKEQKGRNRGIATRGNKVSFGEYGLKATTRGRLTA
                     RQIEAARRAMSRAIKRGGRIWIRIFPDKPISQKPAEVRMGNGKGNPEYWVSEIKPGAV
                     LYEMDGVNEQEAREAFRLASAKLPLRTTVVSRMVGG"
     gene            3270..3473
                     /gene="rpmC"
                     /locus_tag="AC158Q_12265"
     CDS             3270..3473
                     /gene="rpmC"
                     /locus_tag="AC158Q_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HIR3340592.1"
                     /translation="MKAAELKSKAPDELQQELNSLLKAQFSLRMQKATQQLTNTSQLG
                     KVRRDIARVRTVMAEKSKGQGKA"
     gene            3470..3733
                     /gene="rpsQ"
                     /locus_tag="AC158Q_12270"
     CDS             3470..3733
                     /gene="rpsQ"
                     /locus_tag="AC158Q_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HIR3340593.1"
                     /translation="MSEAANKRQLTGRVVSDKMTKTVTVLVERRMKHKLYGKYVTRSS
                     KYHAHDEAAECKAGDLVLIEECRPISKTKAWRVIQLVEKAKIV"
     gene            4015..4383
                     /gene="rplN"
                     /locus_tag="AC158Q_12275"
     CDS             4015..4383
                     /gene="rplN"
                     /locus_tag="AC158Q_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HIR3340594.1"
                     /translation="MIQMQSVLDVADNTGARSVMCIKVLGGSKRRYAGIGDVIKVSVK
                     DAAPRGRVKKGEVYDAVVVRTAKGVRRSDGSLIKFDSNAAVLLTPKLEPIGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            4394..4711
                     /gene="rplX"
                     /locus_tag="AC158Q_12280"
     CDS             4394..4711
                     /gene="rplX"
                     /locus_tag="AC158Q_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003068411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HIR3340595.1"
                     /translation="MHKIRKGDDVIVTTGRDKGKRGTVLRIVDAEHVLVEGTNRVKKH
                     QRPIPMKGITGGITDKEMPLHVSNVALYNPNTKKADRVGIRVLEDGRRVRFFKSNGEV
                     IDA"
     gene            4912..5451
                     /gene="rplE"
                     /locus_tag="AC158Q_12285"
     CDS             4912..5451
                     /gene="rplE"
                     /locus_tag="AC158Q_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HIR3340596.1"
                     /translation="MPRLQIHYREAVVKQMMEQFAYKTSMQVPRIEKIVLNMGVGEAV
                     ADKKIMDNAVADMVKIAGQKPLITAARKSIAVFKVRAGYPVGCKVTLRGRRMYEFLDR
                     LVNIAMPRIRDFRGISGRAFDGRGNYNMGVKEQIIFPEIEYDKIDAVRGMNITITTTA
                     KSDNEAKALLAAFRFPFRN"
     gene            5463..5768
                     /gene="rpsN"
                     /locus_tag="AC158Q_12290"
     CDS             5463..5768
                     /gene="rpsN"
                     /locus_tag="AC158Q_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HIR3340597.1"
                     /translation="MAKTSVVNRDKKRREIVKKYAARRKELLESAGNQKLSPEERYAA
                     REKLQKLPRDASPVRLRNRCALTGRPRGVYRKFGLARGKLRIIALHGEIPGVIKASW"
     gene            5788..6183
                     /gene="rpsH"
                     /locus_tag="AC158Q_12295"
     CDS             5788..6183
                     /gene="rpsH"
                     /locus_tag="AC158Q_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HIR3340598.1"
                     /translation="MSMNDPIADMLTRIRNAQHAEKPTVAMPASKVKAAIAQVLKDEG
                     YVDDFAVRELEGKPTLEISLKYYAGKPVIEKIERVSRPGLRIYKPKDDLPKVMNGLGV
                     AIVSTSRGVMTDRKARGLGVGGDVLCVVA"
     gene            6197..6730
                     /gene="rplF"
                     /locus_tag="AC158Q_12300"
     CDS             6197..6730
                     /gene="rplF"
                     /locus_tag="AC158Q_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HIR3340599.1"
                     /translation="MSRIGNFPIMLPEKVEVAITPVEISVKGPLGTLNQRLTPEVTIE
                     KVENRLQVKAAGNSRQANAMSGTVRALVANMVTGVTKGFEKRLTLVGVGYRAQAQGDK
                     LNLTLGFSHPVAHPMPKGVKVETPTQTEIVIKGMDRQQVGQVAAEVRAYRSPEPYKGK
                     GVRYANEQVVIKETKKK"
     gene            6742..7098
                     /gene="rplR"
                     /locus_tag="AC158Q_12305"
     CDS             6742..7098
                     /gene="rplR"
                     /locus_tag="AC158Q_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HIR3340600.1"
                     /translation="MFDKNQQRLRRARQTRARISELAVVRLTVHRSNSHIYAQVIDAG
                     GSKVLASASSVEKELRGAVKSGGNVKAAAEVGKRIAERAKKLGIEAVAFDRSGYKFHG
                     RVKALAEAAREAGLKF"
     gene            7114..7638
                     /gene="rpsE"
                     /locus_tag="AC158Q_12310"
     CDS             7114..7638
                     /gene="rpsE"
                     /locus_tag="AC158Q_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008642951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HIR3340601.1"
                     /translation="MAKLHASKMQSGEQRGDGLREKMVAVNRVTKVVKGGRVLGFAAL
                     AVVGDGDGGIGMGKGKAREVPVAVQKAMEEARRKLVKVSLKHGTLQHAVIGHHGASRV
                     FMQPASDGTGIIAGGPMRAVFEVMGVTNVLAKCLGSTNPYNVVRATINGLTGMNTPAQ
                     IAAKRGKSVEEITG"
     gene            7641..7838
                     /gene="rpmD"
                     /locus_tag="AC158Q_12315"
     CDS             7641..7838
                     /gene="rpmD"
                     /locus_tag="AC158Q_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HIR3340602.1"
                     /translation="MAKAKSGGKTLKVTLVRSPIGTKQLHRLCVRGLGLRKIRQSVEL
                     EDTPAVRGLINKVAYLVKCEG"
     gene            7840..8352
                     /gene="rplO"
                     /locus_tag="AC158Q_12320"
     CDS             7840..8352
                     /gene="rplO"
                     /locus_tag="AC158Q_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HIR3340603.1"
                     /translation="MRLNDIKPASGSKHYKKRPGRGIGSGLGKTSGRGHKGQKSRAGG
                     YHKVGFEGGQMPLQRRVPKRGFVSHLRKDTAEVRLGDLQKMTAGVIDLAVLKSAGVVP
                     KPALRAKVILAGEIKRKIALKGLLVSKGARAAIEAAGGSVELPAPLEPAGKLKPKAGG
                     EAAAAAAKQG"
     gene            8396..9682
                     /gene="secY"
                     /locus_tag="AC158Q_12325"
     CDS             8396..9682
                     /gene="secY"
                     /locus_tag="AC158Q_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HIR3340604.1"
                     /translation="MGDLKRRLLFLLGALIVFRIGSYIPVPGIDPAQLAELFRSQRGG
                     ILDMFNMFSGGALSRFTIFALGIMPYISASIIMQLMTVVSPTLEALKKEGESGRRKIT
                     QYTRYFTFGLALFQSMGIAIALESQRGLVIDPGIAFRIVTMLTLTTGTMFLMWLGEQI
                     TERGIGNGISLIIFAGIAAGLPEAVAGTLELVRTGAFSILVALVIFALVLLVTAFVCF
                     VERGQRRILVNYAKRQVGNKIYGGQSSHLPLKINMSGVIPPIFASSIILFPATLVGWF
                     GAAEGMNWLKTVAGTLSPGQPVYVMLYASAIIFFCFFYTALVFNPKETADNLKMSGAF
                     VPGIRPGDQTSRHIERIMMRLTLGGAIYVTLVCLLPEFLILKWNVPFYFGGTSLLIIV
                     VVTMDFMSQVQAYMMSHQYESLLKKANFKGGMFPSR"
     gene            9682..9900
                     /gene="infA"
                     /locus_tag="AC158Q_12330"
     CDS             9682..9900
                     /gene="infA"
                     /locus_tag="AC158Q_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018149800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HIR3340605.1"
                     /translation="MAKEETIQMQGEIAETLPNATFRVKLENGHMVLGHISGKMRMHY
                     IRILPGDKVTVELTPYDLTRGRIVFRAK"
     gene            10009..10122
                     /gene="rpmJ"
                     /locus_tag="AC158Q_12335"
     CDS             10009..10122
                     /gene="rpmJ"
                     /locus_tag="AC158Q_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HIR3340606.1"
                     /translation="MKVLASVKRICRNCKVIRRKRVVRVICKNPRHKQRQG"
     gene            10200..10580
                     /gene="rpsM"
                     /locus_tag="AC158Q_12340"
     CDS             10200..10580
                     /gene="rpsM"
                     /locus_tag="AC158Q_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HIR3340607.1"
                     /translation="MARIGGVNIPNHQHAEIALTAILGIGRSRARAICTAAGVSITTK
                     IKDLSDTQMDKLREQVAKFPIEGDLRREVSMNIKRLIDLGTWRGKRHRLGLPVRGQRT
                     RTNARTRKGPRKAAIKIIRQVAAG"
     gene            10596..10985
                     /gene="rpsK"
                     /locus_tag="AC158Q_12345"
     CDS             10596..10985
                     /gene="rpsK"
                     /locus_tag="AC158Q_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015237686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HIR3340608.1"
                     /translation="MAKAATRVRKKTKKNVAEGIAHIHASFNNTIVTITDRQGNTLAW
                     ATCGSNGFKGSRKSTPFAAQVAAGRAGTLAQECGVKNLEVRIKGPGPGRESAVRALNA
                     VGFKITQIADVTPVPHNGCRPPKKRRV"
     gene            11112..11738
                     /gene="rpsD"
                     /locus_tag="AC158Q_12350"
     CDS             11112..11738
                     /gene="rpsD"
                     /locus_tag="AC158Q_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HIR3340609.1"
                     /translation="MARNLDAKCRQCRREGEKLFLKGEKCFTDKCAIERRNYPPGQHG
                     QKNSRLSGYGVQLREKQKVRRIYGLLERQFRKVYHSAEQAKGITGESLLQLLESRLDT
                     VAYRMGFGASRTEARQIVRHNGILVNGKRVSIPSFLCRPGDVIEVTEKAKNQLRIKGA
                     AEAAESRGHPEWLEVDAKALKGKFKARPQRSELPSTINESLVVELYSK"
     gene            11826..12812
                     /locus_tag="AC158Q_12355"
     CDS             11826..12812
                     /locus_tag="AC158Q_12355"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HIR3340610.1"
                     /translation="MPSSAFLKPRIIDVQNISPYHAKLTMEPFERGYGHTLGNALRRT
                     LLSSMPGYAATEVKIGGVLHEYSTIDGVQEDVVDILLNLKGIVLKMHSRDEVLLSLKK
                     SGEGVVTAGDIEPMHDVEIVNPDHVIAHLASGGKLDMQIKVEQGRGYIAATTRRTSTE
                     EGRSVGSILLDASFSPVRRVSYAVEAARVEQRTDLDKLVIDVETNGAIDPEEAVRYAA
                     RILVEQLTVFADLKGLPTQIEEKKATQIDPILLRPVDDLELTVRSANCLKAENIYYIG
                     DLIQRSETELLKTPNLGRKSLNEIKEVLATRGLTLGMRLENWPPAGLEKLTT"
     gene            12915..13355
                     /gene="rplQ"
                     /locus_tag="AC158Q_12360"
     CDS             12915..13355
                     /gene="rplQ"
                     /locus_tag="AC158Q_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HIR3340611.1"
                     /translation="MRHRHGLRKLNRTSSHRLAMFRNMANSLLRHEAIQTTLPKAKEL
                     RRVVEPIITLGKKPSLANRRLAFDRLRDREVVVKVFDELGPRYAKRNGGYLRILKSGF
                     RKGDNAPMAHVELMDRPEEGAATGKDNKEGTQKRAKKEAASKAA"
     gene            13603..>14015
                     /locus_tag="AC158Q_12365"
     CDS             13603..>14015
                     /locus_tag="AC158Q_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HIR3340612.1"
                     /translation="MIFLFTDFGAGDIYVGQMKAVLHERAPKAPVVDLLNDAPAFNVK
                     AGAHLLAAFAARLPKDSVTLAVVDPGVGGPRDPVAVLADGRWFVGPDNGLISVAAARA
                     GKHEVHTIEWRPDRLTASFHGRDLFAPVAAMIAAGG"
BASE COUNT         2998 a         4280 c         4602 g         2135 t
ORIGIN      
        1 aacggtgggt catgccgacc cgctctccct gatgcggcac gccaaggtgc tggttaccag
       61 gggcgcggtc gcgaagatcg aggagctgct cgcatgacga ccgcaacgtc caacacgagg
      121 cagcagcaac gcctgatgca ggtcattctg gcgccggtga tctccgagaa gagcacctat
      181 gttgcggaca agcacaacca ggtcgtgttc cgggttgctc aaaacgcgac caagcccgag
      241 atcaaggccg cggtggagtt catgttcagc accaaggaca agaagctcga ggtggaatcg
      301 gtgaaggtcg tcaacgttcg cggcaaggaa aagcgcttcg ggcagacgat cggccgccgc
      361 agccactgga agaaagccta cgtcagcctc aaggcaggcc aggaaatcaa ctttgccgaa
      421 caggcggagg ccaagtaatg ctggtcaaga tgaaacccgt cacgcccggc ctgcgccagc
      481 aggtgcgggt cgtcaatccc gaactgcaca agggcgggcc ggtgccccac ctcgtcgagc
      541 gcaagaacaa acgaggcggc cgcaacgtgc atggcgagat caccatgcgc caccagggcg
      601 gcggccacaa gcagctgtac cgtaaagtcg acttcaagcg cgacaaggac ggcatcacgg
      661 cgaaggtcga gcgcctcgag tacgatccca accgcagcgc atttctcgcg ctgctatgct
      721 acgccgacgg cgagcggcgc tatatcgtcg cgcccaaggg cgtttccgcg ggcatgcagg
      781 tcatctccgg ggcggaggcg ccgatcaaga acggcaatac cctgccgctg agaaacatcc
      841 cggtcggcac taccattcat tgcatcgaga tgctcccggg caaaggcgct caactcgcgc
      901 gcgccgccgg cgcttcggtg caactgctcg cgcgtgaagg cgactacgcg cagatccggc
      961 tgcgctccgg cgaaatccgc aaggtgcaca tcaactgccg cgcgaccatc ggcgaggtcg
     1021 ggaacgaaga gcattcgctg gaatccatcg gcaaggcggg ccgcgtgcgc tggcgcggca
     1081 tccgtcccac cgtgcgcggc gtggcgcaga acccggtcga ccacccgcac ggcggcggcg
     1141 agggcaagaa agccgcggcg cgcgacccgg tgtctccgtg gggcacgctg tccaagggct
     1201 acaagacgcg caagaacaag cgcaccgatg tgatgatagt ccggcgccgc aacgccaagg
     1261 ccgtgggagg ctaattcatg gcacgctcga tcaagaaggg tccattcgtc gatcttcacc
     1321 tcatcaagaa ggtggagtcg gcgcagaaga ccaatgacaa gcgcccgatc aagacctggt
     1381 cgcgccgctc gaccatcctg cccgatttcg tgggactcac catcgccgtg cacaacggca
     1441 agcagcacgt gccggtatac gtcactgaga acatggtcgg ccacaagctg ggcgagttct
     1501 cgcacacccg cgtcttcaaa ggccactcca aggccggttc tgcgcaggcc gccgctgcgg
     1561 ctgctgcggg cgcagcgccg ggcggcgacc ggaaatcggc gccgacagcg ggtgcggcgc
     1621 cgggggccgc gccggctccg gccgcgggca aggcttaagg gaaacagatg aataccactg
     1681 ccaagctgaa cggcgttcgc ctctcggccc agaagggccg gctggtggcc gacctcatcc
     1741 gcgggaaacc ggtggagcag gcgttgaata tcctgcagtt tgccccccag aagggcgcgg
     1801 tgatcatccg caaagtgctg gagtcggcca tcgccaacgc cgagcacaac gacggcgccg
     1861 atatcgacga gctgaaagtg acgcacattt gcgtcgagcg cggaaccttt ctgcgccgct
     1921 ttcgcgccca gtccaagggc cgtggcgcgg ccataatgaa accgacctgt aacgtcatcg
     1981 ttatggtcgg cgacgggaga aaataaagat gggccaaaaa atcaacccga tcggtttccg
     2041 gcttgcgttg aaccgcaact ggtcctcccg ctggtacgcc aacagcaaga atttccctgg
     2101 catgctgctc gaggacctca gggtccgcga gcatctcaag aagaaactgg cgcatgccgc
     2161 ggtttcccgg gtcgtgatcg agcgcccggc gaagaacgcg cgcatcacga tatacagcgc
     2221 ccgccccggc gtcgtgatcg gcaagaaagg cgaggacatc gagtcgctga agggcgagct
     2281 gcagaagatg ctgggcgtgc cggtgcacgt caatatcgag gaagtgcgca agcccgagac
     2341 cgacgcgcaa ttgatcgcgg actcgatctc gcagcaattg cagaaacgca tcatgttccg
     2401 ccgcgcgatg aagcgcgcga tcaccaatgc gatgcggctg ggcgcccagg gcatcaagat
     2461 cgcgagctcg ggccggttga acggcatcga gatcgcgcgc tacgagtggt accgcgaggg
     2521 acgggtgccg ctgcacacgt tgcgcgcgga gatcgattac ggcgtctccg aggccaagac
     2581 cagctacggc gtcatcggca tcaaggtgtg ggtattcaag ggcgaggtca tgcagaagca
     2641 cgagcagcct gccgccgccg tcgccgcgca gctggcgagc gctgcgaaac ccgaagcggc
     2701 gcccgaggcg ccggcggggg acaagccgaa gaagccgcgc acgccgcgca agccgggcgc
     2761 caagccgggc accagatcag gagcgaagaa tgctgcaacc cgcaaggcgt aagtaccgca
     2821 aggagcagaa gggccgcaac cgcgggatcg caacccgcgg caacaaggtg agcttcggcg
     2881 agtacgggct gaaggcgacc acccgcggac gcctgacggc gcgccagatc gaggcagcgc
     2941 ggcgagccat gtcgcgcgcc atcaagcgcg gcggccgcat ctggattcgc atctttccgg
     3001 acaagccgat ctctcagaaa ccggcggaag tgcgcatggg caacggcaag ggcaatcccg
     3061 aatactgggt ttccgaaatc aagcccgggg cggtactgta cgagatggac ggggtaaacg
     3121 agcaggaggc gcgcgaagca tttcgcctgg cgtccgcaaa gcttcccctg cgcaccacgg
     3181 tcgtttcacg gatggtggga ggttaggcgg ttgaacgagg ggatattccc ctcatggccc
     3241 cctactccag acttagagag aacttgacga tgaaggcagc agaactgaag tcgaaggcgc
     3301 cggacgagct gcagcaggag ttgaactcgc tcctcaaggc gcagttcagc ctgcgcatgc
     3361 agaaggccac ccagcagctg accaacacga gccaactggg caaggtgcgt cgcgacatcg
     3421 cgcgcgtgcg cacggtgatg gcggaaaaat cgaaagggca gggtaaggca tgagcgaagc
     3481 cgcaaacaag cgccagctga ccggccgcgt ggtcagcgac aagatgacga agacggtgac
     3541 cgtgctggtg gagcggcgca tgaagcacaa gctctacggc aagtacgtca cgcgttccag
     3601 caagtatcac gcccacgacg aggcggccga gtgcaaggct ggcgacctgg tgctgatcga
     3661 ggaatgccgc ccgatctcca agaccaaggc ctggcgcgtg atccagctgg tggagaaggc
     3721 caagatagtg tgagggaagg aatgcgaggg ggatttctcc cctcaggccc ctgcaggagg
     3781 ggaattccag ggatcaatac ggtttttgcg ttgcggaatc agcgagatac ccgtataatt
     3841 cgcaaccttt cagcagcgcc cggacgttcc accaagggtg cctgaagcaa gcagattatc
     3901 gctgcacccg gatttgaatt ctggcgtggc gaccgacccg gacgggctcc aatactggcc
     3961 gaatagcgaa agctgagcgg tcaagttgga gaagacagat ttagcgagag aacgatgata
     4021 cagatgcagt ccgtattgga cgtggccgac aacaccggcg cccgctcggt gatgtgcatc
     4081 aaggtgctgg gcggctcgaa gcgccgctac gccggcatcg gcgacgtgat caaggtgagc
     4141 gtgaaggacg ccgcgccgcg cggccgcgtg aagaagggcg aagtctacga tgccgtggtc
     4201 gtgcgaaccg caaagggcgt gcgccgcagc gacggctccc tgatcaagtt cgactcgaac
     4261 gccgctgtgc tgctgacacc caagctcgag ccgatcggca cgcgcatctt cgggcccgtc
     4321 acgcgcgagc tgcgcaccga gcgcttcatg aaaatcgtat cgctcgcccc ggaagtgctg
     4381 taagggagtg atcatgcaca agattcgaaa aggcgacgac gttatcgtga ccaccggccg
     4441 tgacaagggc aagcgcggca ccgtgctgag gatcgtggat gccgagcacg tgctggtcga
     4501 aggcacgaac cgggtgaaaa aacaccagcg cccgattccg atgaaaggca ttaccggcgg
     4561 catcaccgac aaggagatgc cgctgcacgt ctcgaacgtg gcgctctaca acccgaacac
     4621 caagaaggcc gaccgcgtcg gcatccgggt gctggaggac ggccgccgcg tccgtttttt
     4681 caagtccaat ggtgaagtga ttgacgcatg agcgcagcca agaccagaga cggcaaggac
     4741 gccaagggcg ccgccggcaa ggaaggcaag ggccagaagg acgccggcaa aggtcagcag
     4801 cagccggccc ggcctccgaa agaggcgaag caaccgaagg cgccaaggga agcgaaggaa
     4861 accccggagc agaaggagcc gagggaaaga aagccggagc ggcccgcgcc ggtgccgcga
     4921 ctgcagattc attaccgtga agcggtagtg aagcagatga tggagcagtt cgcctacaag
     4981 acttcgatgc aggtgccgcg gatcgagaag atcgtgctga atatgggcgt gggcgaagcg
     5041 gtcgccgaca agaagatcat ggacaacgcg gtggccgaca tggtcaagat cgccggccag
     5101 aagccgctca ttaccgccgc gcgcaagtcg atcgccgtgt tcaaggtccg cgcgggctac
     5161 ccggtgggct gcaaggtcac gctgcgtgga aggcgcatgt acgagttcct cgaccgcctg
     5221 gtcaatatcg cgatgccgcg catccgcgac ttccgcggca tttcgggccg cgccttcgac
     5281 ggcaggggca actacaacat gggcgtcaag gagcagatca tctttcccga gatcgagtac
     5341 gacaagatcg acgcggtccg cggcatgaac atcaccatta ccactacggc aaaatccgac
     5401 aatgaagcga aagcgctact cgcggctttc cgcttcccgt tccggaacta aggcgaacaa
     5461 ctatggcaaa gacttccgtc gtcaatcgcg acaaaaaacg ccgcgagatc gtcaagaaat
     5521 acgccgcgcg gcgcaaggaa ctgctggaat ccgccggcaa ccagaagctc tcgccggagg
     5581 agcgttacgc cgcgcgcgag aagctgcaga agctgccgcg cgatgcttcc ccggtcaggc
     5641 taaggaaccg ctgcgcgctc accggacgcc cgcgcggggt gtaccgcaag ttcggattgg
     5701 cgcgcggcaa gctgcgcatc atcgcgctgc acggcgagat cccgggcgtg atcaaggcga
     5761 gctggtaggg cgaggagaga atcccatatg agcatgaacg atcccatcgc cgacatgttg
     5821 acgcgcatcc gcaatgccca gcatgcggag aagccgacgg tggcaatgcc cgcgagcaag
     5881 gtgaaggcgg cgatcgcgca ggtgctgaag gacgagggct acgtcgatga cttcgccgtg
     5941 cgtgaactcg aaggcaagcc cacgctcgaa atcagtctca agtactacgc cggcaagccg
     6001 gtaatcgaga agatcgagcg ggtgagccgc ccggggctgc gcatctataa gcccaaggac
     6061 gacctgccca aagtgatgaa cgggctgggc gtcgcgatcg tttctacctc gcgcggcgtg
     6121 atgaccgacc gcaaggcgcg cggcctgggc gtcggcggcg acgtgctgtg cgtcgtggca
     6181 taacggagcg cctgccatgt cgagaatcgg aaatttcccc atcatgctgc ccgagaaggt
     6241 cgaagtggcc attacgccgg tcgagatctc ggtgaaaggc ccgcttggta cgctgaacca
     6301 gaggctgacg cccgaggtca ccatcgaaaa ggtggaaaac cgcctccagg tcaaggctgc
     6361 gggcaactcg cgccaggcga acgccatgtc gggaacggtg cgggcgctgg tcgccaacat
     6421 ggtgaccggc gtgaccaagg gcttcgagaa acggctgaca ctagtgggcg tgggttaccg
     6481 cgcgcaggcg cagggtgaca agctgaacct gacgctcggt ttctcgcacc cggtggcgca
     6541 cccgatgccg aagggcgtca aggtggagac gccgacgcag accgagatcg tgatcaaggg
     6601 catggaccgg cagcaggtcg ggcaggtagc ggcggaagtt cgcgcctatc gcagccccga
     6661 gccgtacaag ggcaagggcg tgcgctatgc caacgagcag gtcgtgatca aggaaaccaa
     6721 gaagaagtaa cggggacgcc tatgtttgac aagaaccagc agcgtttgcg cagggcgcgc
     6781 cagacccgcg ccaggatctc ggaactggcc gtggtgcgcc tgaccgtgca tcgctcgaac
     6841 agccacatct acgcgcaggt gatcgatgcg ggcggttcga aggtgctggc ctccgcttcc
     6901 tcggtcgaga aggaattgcg gggtgccgtg aagtccggcg gcaacgtcaa ggcggcggcc
     6961 gaggtcggca agcgcatcgc cgagcgcgcg aagaagctcg gcatcgaagc agttgctttc
     7021 gaccgttcgg gctacaagtt tcacggccgc gtcaaggcgc tggcggaagc cgcgcgcgaa
     7081 gcgggcctca agttctaaag ggatcaggta gccatggcga aattacatgc atccaagatg
     7141 cagtcgggcg agcagcgcgg cgacgggctg cgcgagaaaa tggtcgcggt caaccgcgtc
     7201 accaaagtgg tgaagggcgg gcgcgtgctc ggttttgccg cgctggcagt ggtcggcgat
     7261 ggcgacggcg gcatcggcat gggcaagggc aaggcgcgcg aagtgccggt cgctgtccag
     7321 aaagccatgg aagaagcgcg ccgcaagctg gtcaaggtga gcctcaagca cggcacgctg
     7381 cagcacgccg tgatcgggca ccacggcgcc tcgcgcgtct tcatgcagcc tgcttccgac
     7441 ggcaccggca tcatcgccgg cggcccgatg cgggcggtgt tcgaggtgat gggcgtcacc
     7501 aacgtgctcg ccaaatgcct gggctcgacc aacccctaca acgtggtgcg cgccaccatc
     7561 aacggcctga ccggcatgaa cacgcctgcc cagatcgccg ccaagcgcgg caagtcggtc
     7621 gaagaaatta cgggatgacc atggccaaag cgaaatccgg cggcaaaacc ctcaaggtaa
     7681 cgctggtgag aagccccatc ggcaccaagc agctgcaccg cctgtgcgtg cgcgggctgg
     7741 gcctgcgcaa gatccgccag agcgtggagc tggaagacac gccggcggtg cgcggactca
     7801 tcaacaaggt ggcctacctg gtgaagtgcg agggctgaca tgcggttgaa cgatatcaag
     7861 ccggcttccg gctccaaaca ttacaagaag cgccctggcc gcggcatcgg ctccggcctc
     7921 ggcaagactt cgggacgcgg ccacaagggc cagaaatcgc gcgccggggg ctaccacaag
     7981 gtcggcttcg agggcgggca gatgcccctg cagcgccggg tgccgaagcg cgggttcgtc
     8041 tcccatttgc gcaaagacac cgccgaagtg cgcctcggcg acctgcagaa aatgacggcc
     8101 ggcgtgatcg accttgcggt gctgaagagc gccggcgtag tgccgaaacc tgcgctgcga
     8161 gccaaagtga tattggccgg tgaaatcaag cgcaagatcg cgttgaaggg cctgctggta
     8221 tccaagggcg cccgggccgc catcgaggcc gcaggaggca gcgtcgagtt gccggcgccc
     8281 ctggagccgg cgggcaaact gaaaccgaag gccggcggcg aggccgccgc ggccgcagcg
     8341 aaacaaggat agagcgaact tggccgcctc cgattcggcg ctgggcctgg gcaagatggg
     8401 cgacctgaag cgtcgcctgc tgttcctgct cggcgcgctc atcgtctttc gcatcgggtc
     8461 ctatatcccg gtgccgggta tcgatccggc gcagctggcg gaactgttcc gctcccagcg
     8521 cggcggcatc ctagacatgt tcaacatgtt ctcgggcggc gcgctgtcgc gcttcaccat
     8581 tttcgcgctc ggcatcatgc cctacatctc ggcctcgatc atcatgcaat tgatgaccgt
     8641 ggtttcgccc acgctcgagg ccctgaagaa ggaaggggag tccggccggc gcaagatcac
     8701 gcagtacacg cgctacttca ctttcggcct cgctttgttc cagagcatgg gcattgcaat
     8761 cgcgctggag tcgcagcgcg ggctcgtgat cgacccgggc atcgcgtttc gcatcgtcac
     8821 catgctcacg ctgaccaccg gcaccatgtt cctgatgtgg ctgggagagc agatcaccga
     8881 gcgcggcatc ggcaacggaa tctccctcat catcttcgcc gggatcgccg cggggctgcc
     8941 ggaagcggtt gccggcacgc tcgaactcgt gcgcaccggc gcgttttcca tcctggtggc
     9001 gctcgtgatc ttcgcgctgg tcctgctggt caccgcgttc gtctgctttg tcgagcgcgg
     9061 ccagcgccgc atcctggtca actacgccaa gcgccaggtc ggcaacaaaa tctacggcgg
     9121 ccagagctcg cacctgccgc tgaagatcaa catgtcgggg gtcatcccgc cgatctttgc
     9181 ctcgagcatc atcctgttcc cggccaccct ggtgggctgg ttcggcgcgg ccgagggcat
     9241 gaactggctg aagaccgtcg ccgggacgct gtcccccggc cagccggttt acgtgatgct
     9301 gtacgcatcg gcgatcatct ttttctgctt cttctacacg gcgctcgtgt tcaatcccaa
     9361 ggagaccgcc gataacctga agatgagcgg ggccttcgtc cccggcatcc gcccgggcga
     9421 ccagacctcg cggcacatcg agagaatcat gatgcggctc accctgggcg gagcgatcta
     9481 cgtcacgctg gtgtgcctgc tgccggaatt cctgattttg aagtggaacg tgccgttcta
     9541 ttttggcggc acgtcgcttt tgatcatcgt ggtggtgacc atggacttca tgtcgcaggt
     9601 gcaggcctac atgatgtccc accagtacga gagcctgctc aagaaggcga acttcaaggg
     9661 gggcatgttc ccgtcgcgct gatggccaag gaagaaacca tacagatgca gggcgagatc
     9721 gcagagaccc tgcccaatgc gactttccgg gtgaagctcg agaacggcca tatggtactg
     9781 ggacacatat cggggaagat gcggatgcac tatatccgca tcctgccggg cgacaaggtg
     9841 acggtcgagc tgacgcccta cgatttgacg cgcgggcgca tcgtgttccg ggcgaagtga
     9901 gaattggccg cagaggcgca gagacgcaaa gtgaacaagg cattgaatat ggatcgattc
     9961 ggtttctttg cgccttcgcg gctttgcggc aattaagagg aagcagacat gaaggtattg
    10021 gcatcggtga agagaatctg ccgcaactgc aaggtgatcc gcaggaagcg tgtcgtgcgg
    10081 gtgatctgca agaacccgcg gcacaagcag cgccagggct gatcgggcgg accccgtttt
    10141 ggcttgaaaa ttaacaggtt agacgataaa atcgcaaact ttttgctgcg aggacaggca
    10201 tggcccggat aggcggagtc aacattccga atcaccagca cgctgagatc gccctgaccg
    10261 cgatcctcgg catcggacga tcgcgcgcgc gcgccatttg caccgcggcc ggcgtgagca
    10321 tcacgaccaa gatcaaggac ctctcggaca cgcagatgga caagttgcgt gagcaggtcg
    10381 ccaagttccc gatcgagggt gatctccgcc gcgaggtctc gatgaacatc aagcgcctga
    10441 tcgaccttgg cacctggcgc ggcaagcgcc atcgcctggg gctgccggtg cgcggccagc
    10501 gcacccgcac caacgcgcgc acgcgcaagg gcccgcgcaa ggcggcgatc aagatcatca
    10561 ggcaggttgc tgcaggctaa acaggaaaat tcgatatggc aaaagccgct acccgggtgc
    10621 gcaagaagac gaagaagaac gtggccgaag gcatcgccca catccacgcc tccttcaaca
    10681 acaccatcgt caccatcacc gaccgccagg gcaacacgct tgcctgggcg acgtgcggct
    10741 cgaacggttt caagggttcg cgcaagtcaa cgccgttcgc cgcgcaggtg gcggccggca
    10801 gggcgggcac gctcgcgcag gagtgcggcg tgaagaacct cgaagtgcgc atcaagggtc
    10861 ccggccccgg gcgcgagtcc gcggtgcgcg cgttgaacgc ggtgggtttc aagatcacgc
    10921 aaatagccga cgtgaccccg gtgccgcaca acggctgccg tccgccgaag aagcgtcgcg
    10981 tctaggacct tgagatacga ggggatactc ccctcgtgca cccctacaga tgatagagag
    11041 atagacaggg acgatacgag gggatgctcc cctcgtgcac ccctaaagat gacaaagaga
    11101 tagagacgca catggccaga aatcttgacg cgaaatgcag gcagtgccgc cgggaaggcg
    11161 agaagctgtt tctcaagggc gagaagtgct tcaccgacaa gtgcgccatc gagcgccgca
    11221 actacccccc ggggcagcac ggccagaaga acagccggct gtcgggttac ggcgtgcagc
    11281 tgcgggagaa acagaaagtg cgccgcatct acgggctgct cgagcgccag ttccgcaagg
    11341 tctaccactc tgccgagcag gcgaagggca ttaccggcga gagcctgctg cagctgctgg
    11401 aaagccgcct cgacacggtg gcctaccgca tgggcttcgg ggcttcccgc accgaggcgc
    11461 gccagatcgt gcgtcacaac ggcatactgg tgaacggcaa gcgcgtcagc atcccgtcgt
    11521 tcctgtgccg gccgggcgac gtgatcgaag tcaccgaaaa ggccaagaac cagctgcgca
    11581 tcaagggcgc ggccgaggct gccgagagcc gcggccaccc ggaatggctg gaagtggatg
    11641 caaaggcgct caagggcaag ttcaaggcac ggccgcagcg cagcgaactg ccgtccacca
    11701 tcaacgaatc gctcgtggtc gagctttact cgaaataacc gggcgaagta accgagagca
    11761 gtatctgagc cgtctcacac agccgcagtc acatcgtcgc agcacatcgt aaaggtatcc
    11821 gagccatgcc aagcagcgca tttctcaagc cccgcatcat cgacgtccag aacatttcac
    11881 cgtaccacgc gaagctcacc atggagccgt tcgagcgcgg ctacggacat acgctgggca
    11941 acgcgctccg caggacgctc ctgtcctcga tgcccggcta cgcggcgacc gaggtcaaga
    12001 tcggcggcgt gctgcacgag tactcgacca tcgacggcgt gcaggaggac gtggtcgaca
    12061 tcctgttgaa cctcaagggc atcgtgctga agatgcacag ccgggacgag gtgctgctct
    12121 cgctcaagaa gtcgggcgaa ggcgtggtta ccgccggcga catcgagccg atgcacgacg
    12181 tggagatcgt caatcccgac cacgttatcg cgcacctcgc gtccgggggc aagctcgaca
    12241 tgcagatcaa ggtcgagcag gggcgcggtt acatcgcggc caccacccgc cgcacctcca
    12301 ccgaggaagg gcgcagcgtc ggctcgatcc tgctcgacgc ctccttcagc ccggtgcggc
    12361 gcgtcagcta cgccgtggaa gcggcccgcg tcgagcagcg caccgacctc gacaagctgg
    12421 taatcgacgt cgagaccaac ggcgcgatcg accccgagga agcggtgcgc tatgccgcgc
    12481 gcatcctggt cgagcagctc acggtgttcg ccgacctgaa gggcctgccg acgcagatcg
    12541 aggagaagaa ggcaacccag atcgacccca tcctgctgcg ccccgtggac gatctcgagt
    12601 tgaccgtgcg ctcggcgaac tgcctcaagg ccgagaacat ctattacatc ggggacctga
    12661 tccagcgcag cgagacggaa ctcctgaaga cgcccaatct cggacgcaag tcgctgaacg
    12721 agatcaagga agtgctcgcc acgcgcgggc tcacgctcgg catgaggctg gagaactggc
    12781 cgccggcggg actggagaag ctgaccacgt aggatcaggg atgagggatg agggatgagg
    12841 gatgaggttg aagtaaaacc gaatttccct ccgccctcca ccctccgccc tcatcccttg
    12901 atgaggaacg aaacatgcgt caccgccacg gactcagaaa actcaaccgc accagcagcc
    12961 atcgccttgc gatgttccgc aacatggcga attcgctgct gcgccacgag gcgatccaga
    13021 ccacgttgcc caaggcgaag gagctgcggc gggtggtcga gcccatcatc acgctcggca
    13081 agaagccctc gctcgccaac cgccggctgg cgttcgaccg cctgcgcgac cgcgaagtcg
    13141 tggtcaaggt gttcgacgaa ctggggccgc gctacgcgaa gaggaacggc ggatacctgc
    13201 gcatcctcaa gtccggcttc cgcaagggag acaacgcgcc gatggcgcac gtcgaattga
    13261 tggaccggcc ggaggagggc gccgcaaccg gcaaggacaa caaggagggg actcaaaaaa
    13321 gggcgaagaa ggaggcggct tcaaaggccg cataactcgc accaggtgtg tttttgaaag
    13381 ccggccgaaa gccggctttt ttgttttctg actcgttctt tgtggttcaa ttactgaagt
    13441 tggttttctt ggcgggcttg gcgtcttggc ggctaattaa aggtttttct gcttttttat
    13501 cggcgttcat cccgttccaa tgatctccac cagttcccaa aatttgatac tccgcgacac
    13561 gcctggaaga cagacattgg aacgggattc atcggcggcc gaatgatctt tctctttacc
    13621 gacttcgggg cgggcgacat ctatgtcggc cagatgaaag cggtgctcca cgagcgcgcg
    13681 ccgaaggcgc ccgtggtgga cctgctgaac gacgcaccgg ccttcaacgt aaaagcgggt
    13741 gcgcacctgc tggcggcttt cgccgcgcgg ctgcccaagg acagcgtcac gctggcggtg
    13801 gtggatccgg gggtcggcgg cccgcgcgac ccggtggcgg tgctggccga cgggcgctgg
    13861 ttcgtcggac ccgacaacgg gctcatttcc gtggcggcgg cgcgcgccgg caagcatgaa
    13921 gtccatacca tcgaatggcg gccggacagg ctcaccgcat cgtttcacgg ccgcgacttg
    13981 ttcgcgccgg tggcggcaat gatcgcggcc ggcgg
//
LOCUS       DBNGSG010000469        13108 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3377569, whole genome shotgun sequence.
ACCESSION   DBNGSG010000469 DBNGSG010000000
VERSION     DBNGSG010000469.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 13108)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13108)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13108
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3377569"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2300
                     /locus_tag="AC158Q_12370"
     CDS             <1..2300
                     /locus_tag="AC158Q_12370"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3340613.1"
                     /translation="EYFYSFFGILLAGGVPVPIYPPARASQIEDHLKRHAGILSNALT
                     EVMITVPQAKALSLLLKPRVDTLKYVVTPDELMRPGQAVTIHRGRPQDIAMIQYTSGS
                     TGNPKGVVLTHDNLLVNIRAMAKALRVTPADVFVSWLPLYHDMGLIGAWMGSLIYGFK
                     YPVMSPLTFLARPERWLRTIHRHGGTLSGGPNFCYELCLRRIQDADIQGLDLSSWRFA
                     FNGAEPVSPETMDAFTKRFARYGFRSGAMAPVYGLAEATLGVAFPPPLRGPKLDRVDK
                     NAFMNGGRAVPVPPEHKDALVHVACGLPVPGHQIRIVDGSSRELPERAEGHIQFSGPS
                     VTSGYYRNPEATGDLLDGAWMNTGDYGYVAGGDIHITGRIKDTIIRAGRNIHPYDLEE
                     AVGNLEGIRKGCVAVFGSRDERAGTEKIVVLAETRETDGARREALRGRINELAVALIG
                     TAPDDIMLAPPNTVLKTSSGKIRRAASREVYEHGGGAGTRAVWLQVVRLTWAAILPQA
                     RRSLRAIADNVYGAYALFLLGAMGTLVWVVCSILPRADWDWRFSRRVARAFLWLTGMP
                     LAVRGLEHIPAGRNCLMAVNHASYLDGVLVVAALAQPKTFVAKRELLEHWVPRNFLTA
                     IGAAFVDRLDAQRGVEDTKQFVETAKSGKSLIVFVEGTFRRMPGLLPFRMGAFVVAAQ
                     AGVPVVPVTIRGTRSALRDGQWLFHRSRLSVTFSAPIEPKGNDWNAAIAMRDAARAEI
                     LRLCGEPDLGEETSLPPKPNAKTEN"
     gene            2501..3409
                     /locus_tag="AC158Q_12375"
     CDS             2501..3409
                     /locus_tag="AC158Q_12375"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3340614.1"
                     /translation="MPAQQYPSRPVRIISPFAPGGGNDALCRIVSQRLGENLKQQVIV
                     ENRAGANGIVGTDVAARAAPDGHTIVLIPSGHAVNASLYKKLPFDSIRDFSPITLVGS
                     SPLVLAVHPSLPVKSVKELIALARARPGQLTYVSAGVGASGHLGGALFETLTNTKMTH
                     IPYKGMALAIVDLMSGQVTMTFGTSLSVVPHVRAGRLRALATTGTKRSPALPDLPTVA
                     ETGVPGYEASLWYGFVGPAGMTPQVVQRLNSAIVAVLQMPEIRERLASQGVDPRHNTP
                     QEFAKLLVSDVGRWAKIIQRAGIQPE"
     gene            3582..4601
                     /locus_tag="AC158Q_12380"
     CDS             3582..4601
                     /locus_tag="AC158Q_12380"
                     /inference="COORDINATES: protein motif:HMM:NF014841.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HIR3340615.1"
                     /translation="MPTALVSRQFVARFGDEIKSVAERARQSVSFVTLPATKGAKMPQ
                     AERDRVECIFLDRDMRFEEQLYAAYLDMLLAAPKLKWVHYTSSGVGQQAHVAELGAKG
                     VIVTSSTGSNAEPVAQTGFAGLLMLARGFGSHIHSQCRREWKPLRGDALPDDLRGQTL
                     LLIGVGAVGTVFAGYAKTFGLKVIGVRRSPLKPGEPVDEMHPPSKLPELLPRADWIVI
                     ACPLTKETLDLLNAGSFRRMKKGARLINIGRGEVVNEAALIEALRGGQLGGAALDAHS
                     QEPLPADSPLWSLPNVIISPHNASASTGNEQRCAEMFIANFGHWMRGEPMFNVQDVSK
                     LKVRA"
     gene            4614..5438
                     /locus_tag="AC158Q_12385"
     CDS             4614..5438
                     /locus_tag="AC158Q_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340616.1"
                     /translation="MSDLGTVVLNTRLWAMLCLGALGLTVLLSLFGHFAGPRIWGATQ
                     QDTGAVGQVMLPLFFGLFLLLGFSALPLMANLFVAGLERLWAANGLLERPLNARVMAL
                     VHRHHVHFVLVVWGLFAAGLLIAAPHLIRAWSEEMSAAGSRGAAKGAMPPLEHQVRHA
                     KQILLLRTDIQGGVVRYGVLGLLKQTQSIVATGAPGLLEIDTKSFELLGYQPAHDRQV
                     VMFFSAPDSTGKTLFELLLPAADGMMSYPAYVAPYDPAILKRLTLEELRQLVASQR"
     gene            5606..6820
                     /locus_tag="AC158Q_12390"
     CDS             5606..6820
                     /locus_tag="AC158Q_12390"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3340617.1"
                     /translation="MSQKTLEAESQDRENRATLGAGLFTNGVWDMLSVIVPLYAVAVG
                     LNAAEIGLVVAARSLLPTALSIHGGILMDQLGTRRVLAWVAAGSATLPLLYPVSGWFA
                     VLVALQLFLGLASSLGMAASQTWSMQSSHGDTALLARYTVFSRIGTFLGPVAVGAAWD
                     LFGAWAAFACVALCGAGIVASTAYGSQPATTPPAPAPRAITVLVPRWAQHRQAIALAA
                     IPAVAFILAVSFLRNAPGAIQSSLFVVYLNGIGMSGTLIGFLVALSELFGVFGALAAA
                     RMERWLRGERLLILCIAASITAIAVTPLIGHFLALLIAASAVRGIAQGLSQPLMYSIL
                     SRAAPSTRHGASVGLRNAVVRLASIVTPAAMGFAAEAWGIEASFYVVGAVFLLATAAL
                     AVAARYTLAGRE"
     gene            complement(6904..7920)
                     /locus_tag="AC158Q_12395"
     CDS             complement(6904..7920)
                     /locus_tag="AC158Q_12395"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3340618.1"
                     /translation="MVATRFSRILPACVLRAVICVSAAFLGADICAARQPGHADYPIK
                     PIRIIVASSPGSADDFFARALVAELEAFYRQRIVIENRAGAGGLIGNILLSRANADGY
                     TLGMVGVTRLITELVRDEPPYRSLADIVGVAHVASITNVLAVTPALPVRTAPEFAKYA
                     RARFGELNYASLGIGSASHLAGEMFTRALRVEAMHVPFKNVADTFVEMGLGRVHYAVY
                     TLPAVLAPVREGRLRALAVMTPHRSPTLPAVPAIAEFGLPDAQFDSWSGIVAPRGTPR
                     RVVEQLHGDIVRGLRGAGLRRLFLRQGAESTPESTPDGFMRLMQEEYLRYQAVIREGG
                     IRPE"
     gene            8142..9029
                     /locus_tag="AC158Q_12400"
     CDS             8142..9029
                     /locus_tag="AC158Q_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014749630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR3340619.1"
                     /translation="MERKKASDYPQEVLDLFDGYVHGRMNRREFLDAAAKYAVGGFTA
                     VAMLESLRPNFAWAQQVAKDDKRIQTLQVDYDSPQGSGKMRGYLAKPAKGGKLPGVLV
                     IHENRGLNPYLEDVTRRLAVEGYVAFAPDALAPVGGYPGDEDQARALFAKLDAKKRFE
                     DLYAGAGVIKARPEFNGKIGAVGFCFGGLIVNQLAVRMPELAAGVPFYGRQVSAEDAA
                     KIKAALLLNYAEHDDGVNKGWPAYEAALKANNVRYQAHFYPGTNHGFHNDTTPRYDEA
                     AAKLAWSRTLAFFKTHLKG"
     gene            9121..9828
                     /locus_tag="AC158Q_12405"
     CDS             9121..9828
                     /locus_tag="AC158Q_12405"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR3340620.1"
                     /translation="MASKYMLCSFKTCPWVQRAAIVLRAKQVPYDITYIDRDNRPDWF
                     LKVSPHSKVPVLVIDGKDSLFESNAIAEYLDEAVPPRLHPEDPLTRARNRAWTDYVAT
                     FASAVSDTAYADSEAEFATKAARIADPFGKLDEALASRGNAGPHFNGPGFSLVDAAYA
                     PFLQRYTFMDRLRPLGVIEKFPRLAAWRDALLAAPAVKASTVPNIEAVWQENLILRKR
                     WLGQLVSKSVLGASAAA"
     gene            complement(10076..10255)
                     /locus_tag="AC158Q_12410"
     CDS             complement(10076..10255)
                     /locus_tag="AC158Q_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340621.1"
                     /translation="MLAVHERVPHLYALAIAWIAWVKRPRAFSRLVSRYCGLGAGDGR
                     KAGDRAAEKPADEPS"
     gene            10241..11227
                     /locus_tag="AC158Q_12415"
     CDS             10241..11227
                     /locus_tag="AC158Q_12415"
                     /inference="COORDINATES: protein motif:HMM:NF021497.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HIR3340622.1"
                     /translation="MDSEHVVGHKLLVENDMNSGGDFLSGEKSDRWYTSGIKLITMLQ
                     EDTRPYWLSNALPDFACWWGSNYDFQFGYVLGHMMYTPSNILDPYPQVNDRYWAAWAY
                     VGTIVQFRDKKDPRTLQTFEFDVGITGRPALGEPLQKTIHAIIGSPQPRGWDNQLKSE
                     LAVNMTYLGMYKLGDARRLPLPGNGLKWDATVHGGFALGTVFNHVNAGMTLRIGNSLA
                     GTPIGTIEVPSLGGGDRWEEGNWYAFVRFDQRATAYNLFLDGSLFRTDPHPSTIKSTN
                     TPYFINRGITYESSRKNRWTLTFNRRSREFNAPPSDRGIQNFTTLVWEHRFR"
     gene            11687..12001
                     /locus_tag="AC158Q_12420"
     CDS             11687..12001
                     /locus_tag="AC158Q_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340623.1"
                     /translation="MASVGRTPPIRKQQSRKPLAWEEINEPGAYVEIATGMLYRVPPE
                     VLAGDAAPLVEKQDESGPQLVPVDTRHFVLSRFVRVSGDPYIFSLGARMICVEQDIQP
                     HF"
     gene            12066..12530
                     /locus_tag="AC158Q_12425"
     CDS             12066..12530
                     /locus_tag="AC158Q_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3340624.1"
                     /translation="MIARKRPIHAGRQTATPYLVVHDAVEAIDFYKRAFGAKEVMRIE
                     SNGKIGHAQIRIGNSHIMISDEHPEAGALSPKSYGGSPVRTFLYVKDVDTFVGRAVAI
                     GAKLKQGVDEKIYGDRSGSLEDPFGHFWHVATHRKDVSADEIQQLTNDSQAS"
     gene            complement(12568..>13108)
                     /locus_tag="AC158Q_12430"
     CDS             complement(12568..>13108)
                     /locus_tag="AC158Q_12430"
                     /inference="COORDINATES: protein motif:HMM:NF019410.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HIR3340625.1"
                     /translation="KPAVTVKEPAAATGDAKPAPVAKGAVPPAPAKTGAETTESLDFT
                     STITVKNPFGGGEVKVEEMDFTGGAAPAPGAGRVKRGPSDAELTEKLAEGTWVDYKEK
                     KPGSEEELRQPAKLSYVSPMKARYLFVDRAGKTVLECSRAELARRMRLGDVAVMDEAP
                     LFDRIMGGLVSKMRGAVPN"
BASE COUNT         2274 a         4381 c         4202 g         2251 t
ORIGIN      
        1 tcgagtactt ctacagcttc ttcggcatcc tcctcgcggg cggcgtcccg gtgccgatct
       61 acccgccggc gcgcgcctcc cagatcgagg atcacttgaa gcgccacgcc ggcatccttt
      121 ccaacgcgct gaccgaagtg atgatcaccg tgccgcaggc gaaggcgctc tcgctgctgc
      181 tgaaaccgcg ggtggacacg ctgaagtacg tggtgacgcc cgatgaactg atgcggccgg
      241 ggcaggccgt gacgatccat cgcgggcgcc cgcaggacat cgcgatgatc cagtacacct
      301 ccggcagcac cggcaacccc aagggcgtgg tactcacgca cgacaacctg ctcgtcaaca
      361 tccgcgcgat ggcaaaagcg ctgcgggtga cgccggccga cgtgttcgtg agctggctgc
      421 cgctctatca cgacatgggg ctgatcggcg cctggatggg cagcctgatc tacggcttca
      481 agtacccggt gatgtcgccg ctcaccttcc tcgcgcggcc ggagcgctgg ctgcgcacga
      541 ttcaccgcca cggcggcacg ctctcgggcg ggcccaactt ctgctacgag ctgtgcctgc
      601 gccgcatcca ggacgcggac atccagggcc tcgacctctc cagctggcgc ttcgcgttca
      661 acggcgcgga gccggtatcg ccggagacga tggatgcgtt cacgaaacgc ttcgcacgct
      721 acggcttccg gtccggcgcg atggcgccgg tctacgggct cgcggaagcg acgctcggcg
      781 tcgcgttccc gccgccgctg cgcgggccga agctcgaccg cgtggacaag aatgcgttca
      841 tgaacggcgg acgcgcggtg ccggtgccgc cggagcacaa ggacgcgctc gtccacgtcg
      901 cgtgcggcct gccggtcccc ggccaccaga tacgcatcgt cgacggatcg agccgcgagc
      961 tgccggagcg cgccgagggc cacatccagt tcagcgggcc gtcggtgaca agcggctact
     1021 accgcaaccc ggaggcgacg ggcgatctgc tcgacggcgc gtggatgaat accggcgact
     1081 acggctacgt cgccggaggg gacatccaca tcaccggccg catcaaggac accatcatcc
     1141 gcgccggccg caacatccat ccctacgacc tggaggaggc ggtcggcaac ctcgagggca
     1201 tccgcaaggg ctgcgtcgcc gtgttcggca gccgcgacga gcgcgccggc acggagaaaa
     1261 tcgtcgtgct cgcggagacg cgcgagaccg atggcgccag acgcgaggcg ctgcgcggcc
     1321 ggatcaacga gctcgcggtg gcgctgatcg gcacggcccc ggacgacatc atgctggcgc
     1381 cgcccaacac cgttttgaag acttcgagcg gcaagatccg ccgcgcggcg agccgcgagg
     1441 tgtacgagca cggcggcggc gccggcacgc gggcggtgtg gctgcaggtc gtgcgcctta
     1501 cctgggccgc gatcctgccg caggcgcgac gctcgctgcg cgccatcgct gataatgttt
     1561 acggcgccta cgccctcttc ctgctcggcg cgatgggcac gctggtctgg gtggtctgct
     1621 cgattctgcc gcgcgcggac tgggactgga ggttcagccg ccgggttgcg cgcgcattcc
     1681 tgtggctgac cggtatgccg ctcgcggtgc gcgggctgga acacataccg gcgggccgca
     1741 actgcctgat ggccgtcaac cacgcaagct atctcgacgg cgtcctggtg gttgccgcac
     1801 tggcgcaacc gaagaccttt gtcgccaagc gcgagctgct cgagcactgg gtgccgcgaa
     1861 attttctcac cgccatcggc gccgctttcg tggaccggct cgacgcgcaa cggggggtcg
     1921 aggacacgaa gcagttcgtg gaaacggcga agagcgggaa atcgctgatc gttttcgtgg
     1981 aaggcacctt ccgccgcatg ccggggctgc tgcctttccg gatgggcgcg ttcgtggtcg
     2041 cggcgcaggc cggcgtgccg gtggtgccgg tcacgatccg cggcacccgc tccgccctgc
     2101 gcgacgggca gtggctgttt caccgcagcc ggctctcggt gacgttcagc gcgccgatcg
     2161 agccgaaggg gaacgactgg aatgcggcga tcgcgatgcg cgacgccgct cgcgccgaga
     2221 tcctgcggct gtgcggcgag ccggatctcg gagaggaaac gtcactgccg ccgaagccga
     2281 atgccaagac cgagaattaa ccgccaaggc tctcctgagt atgtcgaagg gacgccagga
     2341 ccgccaggaa attcaagtaa tgggcaccag cctcggccgc cgtcgtctgc ttgaaaccgt
     2401 agctgcaata tcgaaaatat cgggagagaa gatgctgtcg gcacgaataa tgactcttgt
     2461 ggtcgtctcc gcaacagttg ctgcaggatt aagcgcgcag gtgccggctc agcagtatcc
     2521 ctcgcggccg gtgcggatca tctcgccgtt cgcgccgggc gggggcaacg acgccctttg
     2581 ccgcatcgtg tcgcagcggc tgggcgaaaa tctcaagcag caagtgatcg tcgagaaccg
     2641 ggcgggggca aacggcatcg tcgggacgga tgtcgcggcg cgggcggcac cggacggcca
     2701 tacgatcgtg ctgatcccga gcgggcacgc ggtcaacgcg agtctgtaca agaagttgcc
     2761 gtttgattcg atccgggatt tctcgccgat caccctggtc ggctcgagcc ccctcgtcct
     2821 cgccgtgcat ccctcgctgc cggtgaagag cgtgaaagag ttgatcgcgc tggcaagggc
     2881 ccggcccggg caactcactt atgtctcggc tggcgtcggc gcctcgggac acctcggcgg
     2941 cgcgctgttc gagaccctca ccaataccaa gatgacgcac atcccctaca agggcatggc
     3001 gctcgccatc gtcgatctca tgagcgggca ggtcaccatg acattcggca ccagcctctc
     3061 agtggtgccg cacgtgcgcg caggacggct gcgcgcgctc gcgaccacgg gcacgaagcg
     3121 ttcgcccgcc ctgcccgacc ttcccaccgt cgcggaaacc ggcgtgcccg gctacgaggc
     3181 gagcttatgg tacggcttcg ttggccccgc cgggatgacg ccgcaggtcg tgcagcgtct
     3241 caattcggcg atcgtcgccg tgctccagat gcccgagatc cgcgagcgcc tcgcaagcca
     3301 gggcgtggac ccgcggcaca acacgccgca agagttcgcg aagctgctcg tctccgacgt
     3361 cggtcgctgg gcgaaaatca tacaacgcgc cggcatccag cccgagtaac ggtggcggcg
     3421 ccacctgaag tcgaaaggcg aaagtcgaaa gcattgccgg gttttcaccc ccgcactgcg
     3481 gagttcaccc ctcatccctc atctagcttt aggctttcgc aagctgagat atagttcgct
     3541 ttccttccga cttccgactt ccgactttcg actttaaata tatgcccacc gcactggttt
     3601 ccaggcaatt cgtggcccgc ttcggcgacg agatcaagtc cgtcgccgag cgcgcccgcc
     3661 aatccgtttc attcgtgacg ctgcccgcga ccaagggcgc gaagatgccg caggccgagc
     3721 gcgaccgggt cgaatgcatc ttcctcgacc gcgacatgcg tttcgaggaa cagctctatg
     3781 ccgcctacct cgacatgctg ctggcagcgc ccaagctcaa gtgggttcat tacaccagct
     3841 ccggtgtcgg gcagcaggct cacgtggccg agctcggcgc gaaaggtgtg atcgtcacca
     3901 gttccaccgg gtcgaacgcc gagccggtgg cgcagacggg gtttgccggg ctcctcatgc
     3961 tggcccgcgg cttcggcagc cacatccaca gccagtgccg gcgcgaatgg aagccgctgc
     4021 gcggcgatgc gctgccggac gacctgcgcg ggcagacgct gctactgatc ggcgtgggcg
     4081 cggtcggcac cgtcttcgcc ggctatgcga aaacgttcgg cctcaaggtg atcggcgtgc
     4141 gccgcagccc gctgaagcca ggcgagccgg tggacgagat gcatccgccc tcgaagctgc
     4201 cggagctgtt gccccgggcc gactggatcg tcatcgcgtg ccccctgacg aaggagacgc
     4261 tcgacctcct caatgccggc tcgttcaggc gcatgaagaa aggcgccagg ctgatcaaca
     4321 tcgggcgcgg ggaagtggtg aatgaagcgg cgctgatcga ggccctgcgc ggcgggcagc
     4381 tgggcggagc cgcgctcgac gcgcacagcc aggagccgct gcccgcggat tcgccgttgt
     4441 ggagcctgcc caacgtcatc atctcgcccc acaatgcgtc cgcctccacc ggaaacgagc
     4501 agcgctgcgc cgagatgttc atcgccaatt tcggccactg gatgcgcggc gagccgatgt
     4561 tcaacgtgca ggatgtatcg aaactgaagg tcagggcgta gtatgcccat tcaatgtccg
     4621 acctcggcac cgtcgttctc aacacccggc tctgggcaat gctgtgcctg ggcgcgctgg
     4681 ggctgaccgt attgctgtct ctgttcgggc acttcgccgg gccgcgcatc tggggcgcca
     4741 cgcagcaaga cacgggggcc gtgggccagg tcatgctgcc gcttttcttc ggcctgtttc
     4801 tgctgcttgg tttctccgcc cttccgctga tggccaacct gttcgtggcg ggtctggagc
     4861 gcttgtgggc ggccaatggc ctgctggagc ggccgctgaa cgccagggtc atggcgctgg
     4921 tacaccggca tcatgttcac ttcgtgctgg tggtatgggg cttgttcgcg gccggcctgc
     4981 tcatcgccgc gccccacctg atccgcgcct ggagcgagga aatgtccgcg gcgggcagcc
     5041 gtggtgcggc gaagggagcc atgccgccgc tcgaacacca ggtgcgtcat gccaaacaga
     5101 tcctgctgtt gcggacggac atacaaggcg gcgtggtccg ctacggggtg ctgggtctgc
     5161 tgaagcaaac gcagtccatc gtcgcgactg gcgcgccggg gctgctggaa atcgacacaa
     5221 agagctttga actgctggga taccagccgg cgcacgaccg gcaggtggtg atgttcttca
     5281 gcgcgccgga ttcgaccggg aaaacgctgt tcgagctgct gctgccggcg gcggacggaa
     5341 tgatgtccta ccctgcttac gtggcgccct acgatccagc gattttgaag aggctcacgc
     5401 tggaagaatt gcggcagctg gtcgcttcgc agagataaat aaggtcaata gcaaaacccg
     5461 aagaccgcga agcacacgcg caaaaagcgg cctgcaagca gcgctcctgg tcattttgta
     5521 gtcgtagtaa aaccgtcaga cagcgttcct gccttctgcc gtagcccctc atccttcgga
     5581 agttgaggca ctgatactct ggtacatgtc gcaaaaaaca ctcgaagcag agagccagga
     5641 tcgcgaaaac cgcgccacgc tgggtgcggg gctgttcacc aacggcgtgt gggacatgct
     5701 gtccgtcatc gtaccgttgt atgcggttgc cgtcgggctg aacgcggccg agatcggcct
     5761 ggtcgtcgcg gcgaggtcgc tccttcccac cgcgctgtcg atccacggcg gaatcctgat
     5821 ggatcagctc ggtacccgcc gcgtgctggc ctgggtggcg gcgggcagcg cgacgctgcc
     5881 gctgttgtat cccgtatcgg gctggttcgc ggtactggtt gcgctgcagt tgttcctggg
     5941 cctggcttcc agcctcggga tggcagcctc gcaaacctgg agcatgcagt cgagccatgg
     6001 cgacaccgcc ctgctcgcgc gctacaccgt cttctcgcgc atcggcacct ttctcggccc
     6061 cgtcgccgtc ggcgcggcat gggacttgtt cggcgcctgg gccgcgttcg cgtgcgtggc
     6121 gctgtgcggc gccggcatcg tggcgtcaac cgcctacggg tcccaacctg cgacgacgcc
     6181 gcccgcgccg gcaccgcggg caatcaccgt gctggtgccg cgatgggcgc agcacaggca
     6241 ggccatcgcg ctcgcggcga tacccgcggt ggcattcatc cttgcggtca gcttcctgcg
     6301 caacgcgccg ggggcgatac agagttcgct cttcgtggtc tatctcaacg gcatcggcat
     6361 gagcggcacg ttgatcggct tcctcgtcgc gctctccgaa ctcttcggcg tattcggcgc
     6421 cctggcggct gcgcgcatgg agcgctggct gcgcggggaa cggctcctga tcctgtgcat
     6481 tgcggcctcg atcaccgcga tcgcggtcac ccctctcatc ggccactttc tcgccctgct
     6541 gatcgcggcc tcggccgtgc gcggaatcgc gcagggcctc agccagccgc tcatgtattc
     6601 gatcctgagc cgcgcagcgc cttcaacccg gcacggggca agcgtggggc tgcgcaatgc
     6661 cgtggtccgg ctcgcctcca ttgtgacgcc ggctgcgatg ggcttcgccg cggaggcctg
     6721 gggcatcgaa gcgagctttt acgtcgtggg cgcggtgttc ctgctggcga ccgccgcgct
     6781 cgcggtcgcc gcccgctata cgctggcggg ccgggaatag aataaaaatg gacaggttcg
     6841 gcggtgaggc tttaacccaa cccgcattgc gggattcatc ctttcgcctt tcgacttccg
     6901 cctttactcc ggcctgatcc cgccttcgcg gatcacggcc tgatagcgca gatattcctc
     6961 ctgcatcagc cgcatgaagc cgtccggcgt gctctccggg gtagactccg caccctggcg
     7021 caggaacagc ctgcgcagac cggcgccacg cagcccgcgc acgatgtcgc cgtgcaactg
     7081 ctcgacgacg cgccggggcg tgcccctggg cgcgacgatg cccgaccagc tgtcgaactg
     7141 cgcgtcggga agaccgaact cggcgatcgc cggcacggcc ggcagcgtgg ggctgcggtg
     7201 gggcgtcatc accgccagcg cgcgcaaccg cccctcgcgc accggcgcga gcaccgccgg
     7261 cagggtatat acggcgtaat gcacgcgacc gagccccatc tcgacgaaag tatccgccac
     7321 gttcttgaac ggcacgtgca tcgcttcgac gcgcagcgcg cgcgtgaaca tctcgcccgc
     7381 gagatgcgac gccgacccga tgccgagaga ggcatagttg agctcgccga atcgcgcgcg
     7441 cgcgtatttc gcgaactcgg gcgccgtgcg caccgggagc gcgggcgtca cggccagcac
     7501 gttggtgatc gaggcgacgt gcgcgacgcc gacgatgtcc gcgagcgacc ggtacggcgg
     7561 ctcgtcgcgc acgagctcgg taatgaggcg cgtgacgccg accatgccga gcgtgtagcc
     7621 gtcggcgttc gccctcgaca gcaggatgtt gccgatcagc ccgcccgcgc ccgcgcgatt
     7681 ttcgatgacg atgcgttgcc ggtagaaagc ttccagctct gcgaccagcg cgcgcgcgaa
     7741 gaagtcatcc gccgagcccg gagaagacgc gacgatgatg cggatgggct tgatgggata
     7801 gtcggcgtgc cccggctgcc gcgccgcgca aatgtccgcg cccaggaacg cagccgacac
     7861 gcatatcacg gcccgcaaaa cgcaggccgg caggattctc gaaaaacggg ttgctaccat
     7921 gccacccccc gggccggccc acgatagcgc gcacgaccgc gcacagcaag ccggcaaggc
     7981 acggcattca tggcggcatg tcagctaaag aacaggaaaa tcagatggtt gaccgcctcc
     8041 tgccgcaccg gcccggagct attgtcggat tttgctccat ccttcatcct tccgccttca
     8101 tctttggcgt ttcaccccca ccccactgaa ggagtctgca aatggagcgc aaaaaagcca
     8161 gtgattatcc acaggaagtg ctcgatctct tcgacggcta cgtgcacggc cgcatgaacc
     8221 gccgcgaatt cctcgacgcc gccgccaagt acgcggtcgg cggcttcacc gccgtggcca
     8281 tgctggagag cctccgtccc aacttcgcgt gggcgcagca ggtcgcgaag gacgacaagc
     8341 gcatccagac gctgcaagtg gattacgact cgccgcaggg ctcgggcaag atgcgcggtt
     8401 acctcgcgaa gccggcgaag ggcggcaagc tgcccggcgt gctggtgata cacgagaacc
     8461 gcggcctgaa cccctatctc gaagacgtca cgcgccgcct cgccgtcgaa ggctacgtcg
     8521 ccttcgctcc cgacgcgctc gcgccggtcg gcgggtaccc gggcgacgag gaccaggcgc
     8581 gcgccctgtt cgccaagctc gacgccaaga aacggttcga agacctctac gccggcgccg
     8641 gcgtgatcaa ggctcggccc gaattcaacg gcaagatcgg cgcggtcggc ttctgcttcg
     8701 gcggcctcat cgtgaaccag ctcgcggtgc gcatgccgga gctggcggcc ggcgtgccgt
     8761 tctacggccg gcaggtcagc gcggaagacg ccgcgaagat caaggcagcg ctcctgctca
     8821 actacgccga gcacgacgac ggcgtcaaca agggctggcc cgcctacgag gcggcgctca
     8881 aggcgaacaa cgttcgatac caggcgcatt tctatccggg cacgaaccac ggcttccaca
     8941 acgacaccac gccgcgctac gacgaagccg ccgcgaagct ggcgtggtcg cgcaccctcg
     9001 ctttcttcaa aacgcatctg aaaggctgag ggtgtgacct cgtgaaacgg gaaggctttt
     9061 acatttcacg ttttacgatt cacgcaaacc gtcgctcgtt accgcatcac tggaggaata
     9121 atggcgtcca agtacatgct gtgcagcttc aagacctgcc cctgggtgca gcgcgcggcg
     9181 atcgtgctgc gggcgaagca ggtgccttac gacatcacct acatcgaccg cgacaaccgg
     9241 ccggactggt tcctgaaggt ctcgccgcac tcgaaagtgc cggtgctggt gatcgacggc
     9301 aaggattcgc tgttcgagtc gaacgccatc gcggaatacc tggacgaggc cgtcccgccc
     9361 cggctgcatc ccgaggaccc gctgacaagg gcaaggaacc gcgcctggac ggattacgtg
     9421 gcgaccttcg ccagcgcggt gtcggacacg gcgtacgcgg actccgaagc ggagttcgcg
     9481 acgaaggcgg ccaggatcgc cgacccgttc ggcaagctcg acgaagcgct cgccagtcga
     9541 ggcaatgccg gcccccattt caacggcccc gggttctcgc tggtggacgc ggcctacgcg
     9601 ccgttcctcc agcgttacac cttcatggac cggctgcgcc cgctcggcgt catcgaaaaa
     9661 tttccgcgcc tcgctgcctg gcgcgacgct ctcctcgccg ccccggcggt caaggcctcc
     9721 accgttccca acatcgaggc cgtgtggcag gagaacctga tcctgcgcaa gcgctggctg
     9781 ggtcagctcg tatcgaagag tgttctgggc gcctcggcgg ctgcctgaca aggggtaatg
     9841 gtcgataggt ctcccgcaat ggggggccct gtgcgttgcg ttgcgccatc gccgcatcgc
     9901 ttattcaccc gcgtgaaggt cgggaaatag gctgggggcc gttcgaggcg cgcccctggc
     9961 gtgctccttg cttccagttg gctgattcct tctccgcctt tttgacccgg ctttgcgctc
    10021 aagcttctca cgccagtcgt cacggcaact gatatcgctg gatccacgca aggccttacg
    10081 acggctcgtc ggcgggcttt tctgcggcgc gatcacctgc tttcctcccg tcgccggctc
    10141 ccagcccgca ataccgggac acgagccggc tgaacgcgcg cggccgcttg acccatgcaa
    10201 tccacgcgat cgcgagcgca taaagatgag gaacgcgctc atggacagcg agcatgtcgt
    10261 cgggcacaag ctgctcgtcg aaaacgacat gaactcggga ggtgattttc tgtccgggga
    10321 aaaatcggac cgctggtaca ccagcggcat caagctcatc accatgctgc aggaggacac
    10381 ccggccgtac tggctctcga acgcgctgcc cgactttgcc tgctggtggg ggtcgaatta
    10441 cgacttccag ttcggctacg tgctcgggca catgatgtac accccgtcca atatcctgga
    10501 cccttacccc caggtgaacg accggtactg ggccgcgtgg gcctatgtcg ggaccatagt
    10561 tcagttcagg gacaagaagg atccgcgaac gctccagacg tttgaattcg acgtcgggat
    10621 cacgggacgc ccggcgctcg gggagccgct gcagaagacc atccacgcga ttatcgggtc
    10681 gcctcagccg cgcggctggg acaaccagtt gaagagcgag ctcgccgtca acatgaccta
    10741 cctcggcatg tacaagctcg gcgacgccag gcgcctgccg ctgcccggaa acggcctgaa
    10801 atgggacgcc accgtccacg gcgggttcgc cctgggcacc gtattcaacc atgtcaacgc
    10861 gggcatgacg ctgcgcatcg gcaattcgct ggcaggaacg cccatcggca cgatcgaggt
    10921 cccgtcgctg ggcggtggag atcgatggga ggaaggcaac tggtacgcgt tcgtccggtt
    10981 cgatcagcgg gcgacggctt acaacctgtt cctcgacggc agcctctttc gcactgaccc
    11041 tcatccgtcc accatcaaga gcacgaacac accctatttc atcaatcgcg gcatcactta
    11101 tgaatccagc aggaagaacc ggtggacgct gactttcaac cgccgcagca gggaattcaa
    11161 cgcgccgccc agcgaccgcg ggatacagaa cttcaccacg ctcgtctggg aacaccgctt
    11221 ccgctgaagc gacgcatgaa gttgatttgt gatgcaccaa atgcttcgcc ttccgccttt
    11281 cgcctttcgc ctttcgactt tcgactttcg actttattaa gtggcgtcgc cgatcggcta
    11341 cgtgaaatca atccgcgaat caggcggctg ccgggcgtgc cgcgaaccgc gtcgtcgagc
    11401 gacgacactt ccggccggaa tgcaatcgct tgcgtactgg cagcgtctga tttcgcggaa
    11461 cttgtcgcgt taggcatgga tatcgcttcc ggaccgccac aatatagtcc ggcatgcgtg
    11521 tcgagtccgc gtccgggact ccaccacgcg gagcggattg acggacccgt gctccgtcac
    11581 gaacgggtaa taccgccgcc gtaagctgtg catggcggcc cggcccgatt ttcagcggcc
    11641 ctgtccgctg aattgacaac gaaataccgt caagaggagg cacaccatgg cttcagtcgg
    11701 aagaactccc ccgatccgca agcagcagtc ccggaaacca ctcgcgtggg aggaaatcaa
    11761 cgagcccggc gcttacgtgg aaatcgcgac cgggatgctg tatcgcgtcc cgccggaggt
    11821 tctggccggg gacgccgcgc ccctggtcga aaagcaggac gaatcgggtc cacaactcgt
    11881 gcctgtggac accaggcact tcgtcctctc gagattcgtg cgggtgagcg gagatcccta
    11941 catcttttcg ctcggcgcgc ggatgatctg cgtggaacag gacatacagc cgcacttctg
    12001 agccgggccg gcgacccgtc tcgcaggtgt ccatgaatcg cttcatccat tgcaacggag
    12061 aatccgtgat agccaggaaa cgacccatcc atgccggacg ccagaccgcg actccttacc
    12121 tagtcgtcca tgacgccgtg gaggcgatcg acttctacaa gcgcgccttc ggtgccaagg
    12181 aggtgatgcg catcgaatcc aacggcaaga tcgggcacgc gcagatccgc atcggcaact
    12241 ctcacatcat gatctccgac gagcaccccg aagccggggc gctcagcccg aagtcctacg
    12301 gcggctcgcc ggtcaggacc tttctctacg tcaaggacgt ggatacgttc gtcggcaggg
    12361 ccgtcgccat cggcgcaaaa ctcaagcaag gggtggacga aaaaatctac ggcgaccgtt
    12421 ccggcagcct ggaggatccc ttcgggcatt tctggcacgt cgcgacccac aggaaagacg
    12481 tgtccgccga cgagatccag cagctcacca acgactccca ggcgagctag cggtgatgag
    12541 gacgagggag caagccgccc tgccggatca gtttggcacc gctccgcgca ttttgctgac
    12601 caggccgccc atgatgcgat cgaacagcgg cgcctcgtcc atgaccgcta cgtcgccgag
    12661 acgcatgcgg cgcgcgagct cggcgcgcga gcactcgagg acggtttttc ccgcccggtc
    12721 gacgaacagg tagcgggcct tcatcgggct gacgtacgac agcttggcgg gctggcgcag
    12781 ttcctcctcg ctgccgggtt ttttctcctt gtaatccacc caggtccctt ccgccagctt
    12841 ctcggtgagc tccgcgtcgc tcgggccccg tttcacccgg ccggcgcccg gagccggggc
    12901 cgcgccgccc gtgaaatcca tttcctcgac cttcacttcg ccgccgccga acgggttctt
    12961 caccgtgatg gtcgaggtga aatccagtga ttcggtggtt tcggcccctg tttttgccgg
    13021 agccggcggg accgctccct tcgccaccgg ggccggtttg gcatcgcccg ttgccgcggc
    13081 cggctccttg accgtgacag cgggtttc
//
LOCUS       DBNGSG010000470        12371 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3547123, whole genome shotgun sequence.
ACCESSION   DBNGSG010000470 DBNGSG010000000
VERSION     DBNGSG010000470.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 12371)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12371)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12371
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3547123"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="AC158Q_12435"
     CDS             complement(<1..646)
                     /locus_tag="AC158Q_12435"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3340626.1"
                     /translation="MSIATVLLIVLFNMSSFRASKVLVSLFAIELGASQFIIGVMIAM
                     YSLLPALLALQVGKLTDRLGVRVPMLVGSLGVALGLLLPWMFQSMAALYASAALIGVS
                     HMMANVSAQNLVGSAGGAEDRTRNFSNYALVMAVGSFLGPLAGGISIDAFGHARSYLY
                     VAAFPLVPVLILAFARELRAGPRVKSEEEQAVLSTSLLSNPALRRTLIASAMAVT"
     gene            complement(650..1840)
                     /locus_tag="AC158Q_12440"
     CDS             complement(650..1840)
                     /locus_tag="AC158Q_12440"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3340627.1"
                     /translation="MQRDPVPDAPGNSPLPRPLSLYEILAVILLWNAAYKGVRVANTL
                     YALELGASPFDTGVLLAVYGVFPLLLAVHIGKLGDRYGVRLPVVSGTIITALGILLPY
                     FWPTFAMLFVSAAVSGAGFILVQVSMQSLVGSLGSGSARTRNLNLYALIVSVSDLMGP
                     VLAGFSIDHFGYMRTYLHLALLAIPSVLALVYLGHRLPQHATGASGRGQQRMKDLFRN
                     RGLRRMLVTSALVMGGLDLFQLYLPIHGHAIGLPASAIGLIMGAFAAAAFLTRALLPL
                     LVRRFGEQTTLCYSMFLSAGIFSLIPMFENAAVLGAACFVLGLGMGLGQPLTVILTYN
                     YSPPGRHGEGLGLRIAINNSMHVTVPAVFGAIGSLLGLAPVFWASSAMLALGGYLGRA
                     QRTG"
     gene            1908..2846
                     /locus_tag="AC158Q_12445"
     CDS             1908..2846
                     /locus_tag="AC158Q_12445"
                     /inference="COORDINATES: protein motif:HMM:NF025008.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase domain-containing protein"
                     /protein_id="HIR3340628.1"
                     /translation="MNNNSSHAAETILRQALLALAANRTPGFHFPGHLLGCHWPRIGE
                     DDLDAAMPDGPQARNVDGTVSLAGLCVLLDVALATAARLNVERGARQATVHLHAQFTG
                     APLRGGLRAHARMEGYTTGATAREGMTSGTLYADNTAVCHASGTFIQLPPPPGVTLAP
                     LPWQREGGAPSFPLKLKDLEPEEKQVMRACRSALRRADGQRAFIEHFWGALPVPSTDG
                     ARCRVRIGPQHGNRVRHVQGGLLFGLAAITARAAAPQHPRLSALSAWFISPGQGSVLK
                     IASRRFHEGRSFAAVRTEIRNADGSRVLELVSHHAA"
     gene            complement(2964..3182)
                     /locus_tag="AC158Q_12450"
     CDS             complement(2964..3182)
                     /locus_tag="AC158Q_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340629.1"
                     /translation="MNATKIAGIVLIVAGTLGLIYGSFSYTKEKHDVKLGPLQFSVSE
                     KETVNVPVWAGAGAIGLGVILLLIGKKP"
     gene            complement(3330..4319)
                     /locus_tag="AC158Q_12455"
     CDS             complement(3330..4319)
                     /locus_tag="AC158Q_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3340630.1"
                     /translation="MNHSSFIAPRSSLVLLCAALLAAALPATAQQYPSRPVRFVVPFA
                     PGGSTDTLARTMGVKLADALGQQVVVDNRPGGNGDIGMLIVARAPADGHTIVLGYIAN
                     LAIAPSLQAKMPYDPVKDFAPITQPASSPNVLTAHPSVQARNLKELIALAKASPGKVS
                     FASTGVASIGHLTGELINNLAAIRMTHVPYKGSGQAVTDILGGHVQVMFSGFSSTLHH
                     IKAGKLRALAVTGPKRAPALSDVPTIAEQGFPGVEATAWYGVLAPAGTPRPVVMRLHG
                     DLVRSLRQPDVVQKLDGLGFEIVGSTPEQFGAYIRSETRKWEKVVRASGAKSD"
     gene            complement(4334..4537)
                     /locus_tag="AC158Q_12460"
     CDS             complement(4334..4537)
                     /locus_tag="AC158Q_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340631.1"
                     /translation="MKLREILKSPVFPLGKKWELKRRPKGYESDVTALVRGMLEDESI
                     REDQRWAWERWRNDADALKNKQG"
     gene            complement(4627..4989)
                     /locus_tag="AC158Q_12465"
     CDS             complement(4627..4989)
                     /locus_tag="AC158Q_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340632.1"
                     /translation="MRVVLGLLLCVPLAGCYVNMHGVQSTNGGTTTTVTATQVSGLAK
                     FGNGTVAFSSGQVPPPGAPGGHLRWSGSASGAILAGVVVAELLGHLVGWNRPKPLPEG
                     TLIMHTCSCFQQPASGEQ"
     gene            complement(5033..6235)
                     /locus_tag="AC158Q_12470"
     CDS             complement(5033..6235)
                     /locus_tag="AC158Q_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340633.1"
                     /translation="MNRRQFLILLAALAVLGAAGLALVWQDISAYRTSGAKIGAKLLP
                     SLKIADVAQVRLQDAKHQATLVRKEKTWVVQERGGYPASFQEISDLLIKLVELKVTQS
                     EQVGASLWPRLELADPAGGGGTAEPGRGEGAGTKMEGAGTTKEGAGTLIEFKDAAGKP
                     LARLTLGKKVLKKDPLNPLPSAKDGVPAGRYVRVDGAGDTVIVVSDPLEKAVAEPGRW
                     LTKDFIKLDRIRTLAVGPQGGALRWKIARSEEWGQWKFATGGGELHPSAAVMAVNKLG
                     QLSFNDVSVDPAAEATGKPLVAVAETFDNLVYTLKLAKRTKGDDYLVTFTLTGEPPRT
                     RVPEKGEKPQDKERNDKAFAESLKRLEGRIAIEKALSQWAYVVAGSELEPLLRGRNEM
                     IARREPRP"
     gene            complement(6232..8142)
                     /locus_tag="AC158Q_12475"
     CDS             complement(6232..8142)
                     /locus_tag="AC158Q_12475"
                     /inference="COORDINATES: protein motif:HMM:NF021351.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HIR3340634.1"
                     /translation="MSKKNLETLLYSTGGVIALVVVLIAANFIMGAFNLRADLTEGDV
                     YTLSPGTRGVLAKLEAPVKLRLYYSQGSEAVPVGLKTFAKRVEDLLNEYRAAAKGKVI
                     IEKFNPEPDSDAEDSAQLDGIEGQLTNTGEKFYLGLSIAFLDQKAAIPVLTPDRERLL
                     EYDITRGIAQVGEARKPVVGVLSALPVMGRSLDPVRKQQPTEPWIFAQELKKIFDVRE
                     VKFDAQKIDDDIKVLVVIHPRNLSEETEYAIDQFVLRGGKLIALVDPYAYFDQQPDLQ
                     NPFGGNQAGQSTFYNLFKAWGVDVDMGKVIADLTYASGEGPRLLPTLLSLNAQALNLD
                     DVVASQVGTLLVPFGGAFKNKLADGLKQTVLARTSKNSMPVDLIIATLSGEPSTRGFQ
                     PSGEEMPLAIRLTGKFHSAFPEGKPRPYAPPRADRKDGKKKDENKDGPKAEPHLKQSA
                     EENSVALIADVDFLSDGAAVEIQEVFGQRVAVPRNGHLALALGLVEHFSGGNALIGLR
                     SRAAFTKPLTVIREIEARAQQQYLGKIKELEDSLNQVQENLKKLQSSKAGATSSILTA
                     EQQTELENFRSKAVETRRVLKELRKNLRVETDALEFWTKVVNISLVPLLVALAGLGLA
                     LARRRRVHQAAA"
     gene            complement(8152..8895)
                     /locus_tag="AC158Q_12480"
     CDS             complement(8152..8895)
                     /locus_tag="AC158Q_12480"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3340635.1"
                     /translation="MNAWANIKAIVKRELGGYFTSPIAYVFLVIFLLLTGFFTFTVGS
                     FFERGEASLVSFFTWHPWLYLFLVPAVGMRLWSEERRLGTLELLLTLPITTWQAIVGK
                     FLASWLFLALALVLTFPVVITVNWLGDPDNGVIFAGYVGSLMLAGSYLAVSCMTSAMT
                     RNQVISFILSVLICLFLILAGYEPVTGLLSRVTSPQVVEVIAAFSVMTHFEGFQRGVL
                     DTRDVVFFLSVMGFALFTTGVIIRNQSAG"
     gene            complement(8892..9626)
                     /locus_tag="AC158Q_12485"
     CDS             complement(8892..9626)
                     /locus_tag="AC158Q_12485"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3340636.1"
                     /translation="MIKVENLVKAFGAKLAVNDVSFTVERGEVLGFLGPNGAGKSTTM
                     RMVTGFIPPTSGRITVGGHDIVEEPLAAKRLFGYLPENAPGYADMTVHGFLNFCAELR
                     GLQGDARKKAVNRVVELCFLENVLYQSVDTLSKGYKHRTCLAQSLIHDPDVLIMDEPT
                     DGLDPNQKHEVRNLIKHMGEKKAIIFSTHILEEVEAACSRVIIIDRGRLVASGTPQEL
                     KARSPEGRLEDVFRAITLPDTARSAA"
     gene            complement(9849..9923)
                     /locus_tag="AC158Q_12490"
     tRNA            complement(9849..9923)
                     /locus_tag="AC158Q_12490"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9889..9891),aa:Thr,seq:tgt)
     gene            10064..10957
                     /locus_tag="AC158Q_12495"
     CDS             10064..10957
                     /locus_tag="AC158Q_12495"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3340637.1"
                     /translation="MTEPRRRVGVIGIGLMGTACAKRLLEAGFDVLGHDVDATKLKPL
                     EALGGRGAGSVSEVAQACDRVVLAVFNTDQVEEAIEGPRGLLAGRPADAAPATVICVS
                     TCDPDRIAALAARLPAGRLRFVEAPVSGTSEQVARGDALGLIGGDPVAVDTARDVIAA
                     ICPRWHAIGPAGSGGRAKLAINLILGINRAALAEGLVFAERMGLDPAAFLSVARESAA
                     YSQIMDVKGGKMIAGDFSPQGYVTQSLKDFSLMLAQARRVKQRLPLGEAYKGLMDGCI
                     AAGEGELDNSAVIREIRRRTG"
     gene            complement(11128..11535)
                     /locus_tag="AC158Q_12500"
     CDS             complement(11128..11535)
                     /locus_tag="AC158Q_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012763106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HIR3340638.1"
                     /translation="MAEKSTKPYLIRAIHEWCTDSGLTPYLSVQVDAQTRVPAEHVKN
                     GEIVLNVGNDATHRLTIGNDLIQFSARFSGVSRECSIPIEAVTGIFAKENGQGLFFPR
                     ETAPNGTSVSPPPETSPPATTPNGGKPKLQVIK"
     gene            complement(11578..12177)
                     /locus_tag="AC158Q_12505"
     CDS             complement(11578..12177)
                     /locus_tag="AC158Q_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006999756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HIR3340639.1"
                     /translation="MMTLYSGTTCPFSQRCRIVLYEKGMDFQIVDVDLYNKPEDLAVM
                     NPYNQVPVLVERDLILYQSNIINEYIDDRFPHPQLMPADPVMRARARLFLYRFENEMF
                     AHIEAIEKGTQKQAEKGRAIIRDSLTQIAPVFSKQKHMLGEEFTMLDVAIAPLLWRLD
                     HYGISLPKAAAPLMKYAERLFSRPAFIDALTASEKVMRK"
BASE COUNT         2124 a         4061 c         4018 g         2168 t
ORIGIN      
        1 cggtcaccgc catcgcgctc gcgatcagcg tccggcgcag ggccgggttg gagagcaggc
       61 tcgtggagag cacggcctgt tcctcctcgc ttttcacccg cgggccggcg cgcagttcgc
      121 gggcgaaggc gaggatcagg accgggacca gcggaaacgc ggcgacgtac aggtagctcc
      181 tggcatgccc gaaggcgtcg atggaaatcc cgcccgccag cggcccgagg aaggacccga
      241 ccgccatgac cagcgcgtag ttgctgaaat tgcgggtgcg gtcctcggcg ccgccggcgg
      301 aaccgacgag gttctgcgcg gacacattgg ccatcatgtg cgagacgccg atcagcgccg
      361 cggatgcgta caaagcggcc atcgactgga acatccacgg caacagcaat cccagggcga
      421 cgccgagcga accgaccagc atgggcacgc gcaccccgag gcggtccgtg agcttgccga
      481 cctggagcgc gagcagcgcc ggcagcagcg agtacatcgc gatcatgacg ccgatgatga
      541 actgggaggc gccgagctcg atcgcgaaca gcgacaccag caccttgctc gcgcgaaagc
      601 tcgacatgtt gaacagcacg atcaggagga cggtggcgat cgacatcgat taccctgtcc
      661 tctgcgctcg gcccaggtag ccgcccagcg cgagcatcgc cgaactcgcc cagaacaccg
      721 gcgcgagccc gagcagggag ccgatcgcgc cgaacaccgc aggcaccgtt acgtgcatgg
      781 aattgttgat cgcgatccgc aggccgaggc cttcgccgtg ccgtcccggg ggcgaatagt
      841 tgtaggtcag gatcaccgtc agcggctggc cgagccccat gcccagcccg agcacgaagc
      901 aggccgcgcc cagcacggcc gcgttctcga acatcggtat cagggaaaaa atgccggcgg
      961 agaggaacat cgagtagcag agcgtcgtct gctccccgaa acggcgcacg agcaggggaa
     1021 gcagcgcccg cgtcaggaac gccgccgccg cgaacgcccc catgatcagg ccgatggccg
     1081 aggccggcag cccgatcgca tggccatgga tcggcagata gagctggaac aggtcgagac
     1141 cgcccatgac gagcgcgctc gtcacgagca tgcggcgcag gccccggttg cggaacaggt
     1201 ccttcatgcg ctgctggccg cggccggatg cgccggtagc gtgctggggc aggcggtggc
     1261 ccaggtaaac cagggccagg acggaaggga tggcgagcag ggcgaggtgc agataagtgc
     1321 gcatgtaccc gaagtggtct atcgagaagc ccgcgagtac cggccccatc aggtcggaga
     1381 cggacacgat cagcgcgtac agattgaggt tgcgggtgcg cgcggagccg ctgccgagcg
     1441 agccgaccag ggactgcatg gatacctgga ccaggatgaa cccggcgccg ctcacggccg
     1501 cggagacgaa aagcatcgcg aaggtgggcc agaaatacgg gagcagtatc ccgagcgcgg
     1561 tgatgatggt gccggagacg acgggcaggc gcacgccgta gcggtcgccg agcttgccga
     1621 tgtgaaccgc gagcagcagc gggaatacgc cgtagacggc gagcaatacc cccgtatcga
     1681 aagggctggc gcccagttcc agcgcgtaga gcgtgttggc cacgcgcacg cccttgtagg
     1741 ccgcgttcca cagcagtatg acggcgagga tctcgtagag gctcagggga cggggaagcg
     1801 gggaattgcc cggtgcgtcc ggtactgggt cacgctgcat ttcgcattcg attttaccgc
     1861 taatgcgagc agccgatgac gcggcctgca tgatttagaa tcgtgtcatg aacaacaatt
     1921 cctcacacgc tgccgaaacg atcctccgtc aggcgctgct cgccctggcg gcgaatcgca
     1981 cgccgggctt ccactttccc ggccacctcc tgggatgtca ctggccgcgc atcggtgagg
     2041 acgacctcga cgccgccatg cccgacggcc cgcaggcccg caatgtggac ggcacggtca
     2101 gcctcgcggg cctgtgcgtg ctgctcgacg tcgcgctcgc caccgccgcg cgcctgaacg
     2161 tcgagcgcgg cgcgcgtcag gccacggtgc acctgcacgc ccagttcacc ggcgctccgc
     2221 tgcgcggcgg gctgcgggcg catgcgcgca tggagggcta cacgaccggc gccacggcgc
     2281 gtgagggcat gaccagcgga acgctgtacg cggacaacac ggcggtatgc cacgcaagcg
     2341 gcacgttcat ccagctgccg ccgccgcccg gcgtcacgct cgcgccattg ccgtggcagc
     2401 gggaaggcgg cgcgccatcc ttcccgctga aattgaagga cctcgaaccc gaggagaagc
     2461 aggtgatgcg ggcgtgccgt tccgctttaa ggcgcgctga cggccagcgc gccttcatcg
     2521 agcacttctg gggcgcactg cccgtgccgt ccacagacgg cgcgcgctgc cgcgtgcgga
     2581 tcggcccgca acacggcaac cgggtaaggc atgtgcaggg cggcctgctg ttcggcctgg
     2641 cggccatcac tgcgcgggcc gcggcgcctc aacacccccg gctctccgcc ctctccgcct
     2701 ggttcatcag ccccggacag ggcagtgtgc tgaaaatcgc ctcgagacgg ttccacgaag
     2761 gccgcagctt cgccgcggta cggacggaaa tcaggaacgc cgacggttca cgcgtgctgg
     2821 agctcgtcag tcaccatgct gcctgacgac ggtgacgcag tgacgaagtg acgcggtgac
     2881 gggcaatcaa accaagtgaa tagcaacttc gagtccgttt attcttcgtc acccgtatcc
     2941 cgtcactttt tctcgctccg cagctacggt ttttttccga tcaaaagcag gatcacgcca
     3001 aggccgattg ctcccgcgcc ggcccacacg ggcacgttca cggtttcctt ttccgacact
     3061 gagaactgca gcggcccgag cttcacgtcg tgcttttcct tggtgtagct gaaactgccg
     3121 tagatcaatc ccagcgttcc cgcgacgatc agcacgatcc cggcgatttt cgttgcattc
     3181 atgcatgcct cctggtgtgc gcggttctac ccatcactgg tcactgattc tcacccctca
     3241 tccctccgcc ccgatgcgct gcatcgcatc gggcgttcac cggccggaac gtgcgcaggc
     3301 acgttcttcg aaaccccggc tcactccctc tagtcagact tcgcccccga agcccgcacg
     3361 accttctccc acttcctggt ttcggaacgg atataggcgc cgaactgctc cggtgtcgag
     3421 ccgacgatct cgaatcccag cccgtccagc ttctggacaa catcgggctg cctgaggctc
     3481 ctgacgagat ccccgtgcag gcgcatgacg acgggcctgg gcgtccccgc gggcgcaagc
     3541 acgccatacc acgcggttgc ttccactccc gggaaaccct gctccgcgat cgtcggcacg
     3601 tcggagagcg ccggcgcgcg cttcgggccg gtgaccgcga gcgcgcgcaa tttcccggcc
     3661 ttgatgtggt gcagcgtgga ggaaaaaccg ctgaacatga cctgcacgtg cccgccgaga
     3721 atgtcggtca ccgcctggcc gctgcccttg tacggaacgt gcgtcatcct gatggcggcc
     3781 agattgttga tgagttcccc ggtgaggtgc ccgatgctgg cgacgccggt cgaggcgaaa
     3841 ctgaccttgc cggggctcgc tttcgcgagc gcgatgagtt ccttcagatt cctggcctgc
     3901 accgacgggt gcgctgtcag cacgttcggc gaggaggccg gctgggtgat gggcgcgaag
     3961 tccttgaccg ggtcatacgg catcttcgcc tggaggctgg gcgcaatcgc gaggttggcg
     4021 atgtaaccga gcacgatggt gtgcccgtcg gccggcgccc tggcgacgat cagcatcccg
     4081 atgtcgccgt tgccgcccgg gcggttgtcc accaccactt gctgcccgag cgcgtcggca
     4141 agcttcacgc ccatggtgcg ggcaagcgtg tcggtgctgc cgcccggcgc gaacggcacc
     4201 acgaaccgca ccgggcgcga ggggtattgc tgcgcggttg cgggcaacgc ggccgcaagc
     4261 agcgccgcgc acagcaatac caatgatgaa cgaggggcaa tgaatgatga atggttcatt
     4321 cgggtaaatc ctgttaaccc tgtttattct tcagcgcatc ggcgtcgttg cgccagcgtt
     4381 cccacgccca gcgctggtcc tcgcgaatgg attcgtcctc gagcatgccc cgcaccagcg
     4441 cggtcacgtc ggattcatag cccttcggcc gccgcttcag ctcccacttc ttgccgagcg
     4501 ggaataccgg agatttgagt atttcgcgta gtttcataag agcgtgaatc gtgattggtg
     4561 attagtgaat ggatcaggca atgatgatgc gggcggctct cgtccccggt tcagtccatt
     4621 caccactcac tgttcaccac tcgcgggttg ctgaaagcag gaacacgtat gcatgatcag
     4681 ggttccctcg ggcaacggct tgggcctgtt ccagccgacg aggtggccga gcagttcggc
     4741 gaccacgacg ccggccaata tcgcgccgct ggcgctcccg ctccagcgca gatgtccgcc
     4801 gggagcgccg ggcggtggaa cctgcccgga ggaaaacgcc acggtgccgt ttccgaactt
     4861 ggccaggccg ctgacctggg tggccgtcac cgtcgtcgtc gtgccgccgt tcgtggactg
     4921 cacgccatgc atgttcacgt agcagccagc cagcggaacg cagagcagga ggcccagaac
     4981 aactctcatg accggagtat aacgcgccac tccccggcgg gcgaaccgcc cgctacggcc
     5041 tgggttcccg cctcgcgatc atttcattgc gccctcggag gagcggctcg agctcgctcc
     5101 cggcaacgac ataggcccac tggctgagcg ctttttcaat cgcgatgcgc ccctcgagcc
     5161 gcttgaggct ctcggcgaac gccttgtcgt tgcgttcctt gtcctgcggc ttctcgccct
     5221 tctccggcac gcgggtcctg ggcggctcgc ccgtcagcgt gaacgtcacg aggtagtcgt
     5281 cgcccttcgt tcttttcgcg agcttcagcg tgtagacgag gttgtcgaag gtttcggcga
     5341 cggcgacgag cggcttcccg gtcgcctccg ccgcgggatc gaccgacacg tcgttgaacg
     5401 agagctgccc gagcttgttc accgccatga ccgcggcgct cgggtgaagc tccccgccgc
     5461 ccgtcgcgaa tttccactgg ccccactcct cgctgcgcgc gatcttccag cggagggcgc
     5521 ccccctgcgg cccgaccgcg agcgtccgga tgcggtccag cttgatgaaa tccttcgtga
     5581 gccagcggcc gggttcggcc actgccttct ccagcggatc ggacaccacg atcaccgtgt
     5641 cgccggcccc gtccacccgg acgtaacggc cagcgggcac gccgtccttg gcggagggca
     5701 gcggattgag cggatccttc ttgagcactt tcttgccgag ggtgaggcgc gcgagcggct
     5761 tgcccgcggc gtccttgaat tcgatcaagg tgcctgcgcc ttcctttgtg gtgcctgcac
     5821 cttccatttt ggtgcccgcg ccctcccccc gacccggctc cgctgtgcct cctcccccgg
     5881 cgggatcggc gagttccagc cgcggccaga gcgacgcgcc gacctgctcg gattgcgtca
     5941 ccttgagctc gaccagcttg atgaggaggt cgctgatctc ctggaagctg gcggggtagc
     6001 cgccgcgttc ctgcaccacc caggtctttt ccttgcgcac cagcgtggcc tggtgcttcg
     6061 cgtcctggag ccggacctgg gcgacgtcgg cgatcttgag ggacggaagc agtttcgcgc
     6121 cgatcttcgc cccgctcgtc cggtaagccg agatgtcctg ccatacgagc gcgagcccgg
     6181 ccgcgcccag cactgccagg gcggcgagca gtatcaggaa ctgcctgcgg ttcatgcggc
     6241 cgcctggtga acgcggcggc gcctcgccag cgcgagcccg aggccggcga gcgccaccag
     6301 cagcggaacc aggctgatgt tgacgacctt ggtccagaac tcgagcgcgt cggtttcgac
     6361 gcgcaggttc ttcctcagct ccttcaacac gcggcgcgtc tcgacggcct tggagcggaa
     6421 attctccagc tccgtctgct gctcggcggt caggatggaa ctggtcgcgc cggccttcga
     6481 gctttgcagc ttcttcaggt tttcctgcac ctggttcaga ctgtcctcca gctccttgat
     6541 cttgccgagg tactgctgct gggcgcgcgc ctcgatctcg cggatcaccg tgagcggctt
     6601 agtgaacgcg gcgcggctcc tcagcccgat cagcgcgttg ccgccggaga aatgctcgac
     6661 caggcccagc gcgagcgcga ggtgaccgtt gcgcggcacg gcgacgcgct ggccgaacac
     6721 ttcctggatc tcgacggcgg caccgtcgga caggaaatcc acgtcggcga tcagggccac
     6781 ggagttttcc tccgccgatt gcttgaggtg cggctcggcc ttgggcccgt ctttattctc
     6841 atccttcttc ttcccgtcct tcctgtcggc gcgcggcggg gcgtagggcc tgggcttgcc
     6901 ctcggggaag gcgctgtgga acttgccggt caggcggatc gcgagcggca tctcctcacc
     6961 cgagggctgg aacccgcgcg tcgagggctc gcccgacagc gtcgcgatga tgaggtccac
     7021 gggcatcgag ttcttggaag tcctcgcgag cacggtctgc ttgaggccgt ccgcgagctt
     7081 gttcttgaac gcgccgccga agggcaccag cagcgtgccg acctggctcg ccaccacgtc
     7141 gtcgaggttc agcgcctgcg cattgagcga cagcagcgtc ggcagcagtc tcggcccctc
     7201 gccgctcgcg taggtgaggt cggcgatcac cttgcccatg tccacgtcca cgccccaggc
     7261 cttgaacagg ttgtagaacg tcgactgccc ggcctggttg ccgccgaacg ggttctggag
     7321 gtcgggctgc tggtcgaagt aggcgtaggg gtccaccagg gcaatcagct tgcccccgcg
     7381 cagcacgaac tggtcgatcg cgtattccgt ttcctcggag aggttgcgcg ggtggatcac
     7441 caccagcacc ttgatgtcat cgtcgatctt ctgcgcgtcg aacttcacct cgcgcacgtc
     7501 gaaaatcttc ttcagctcct gggcgaagat ccacggctcg gtcggctgct gtttcctgac
     7561 cgggtcgagc gagcgcccca tcaccggcag cgcgctcagc acgcccacga ccggtttcct
     7621 ggcttcgccc acctgggcga tgccgcgcgt gatgtcgtac tcgagcagcc gctcgcggtc
     7681 gggcgtcagc accgggatcg ccgccttctg gtcgagaaaa gcgatcgaga gcccgagata
     7741 gaacttctcc ccggtgttgg tgagctggcc ttcgatgccg tccagctgcg cggaatcctc
     7801 ggcgtcggaa tccggctcgg ggttgaattt ctcgatgatc accttgccct tcgcggccgc
     7861 ccggtactcg ttcagcaggt cttccacccg cttggcgaaa gtcttcagcc ccaccggcac
     7921 cgcctcgctg ccctgggaat agtagaggcg cagcttgacc ggcgcctcga gcttggccag
     7981 cactccgcgg gtgccgggcg agagcgtgta gacgtcgccc tcggtcagat cggcacgcag
     8041 gttgaaggcg cccatgatga agttcgccgc gatcagcacc acgaccagcg cgatcacgcc
     8101 gccggtcgaa taaaggaggg tttccaggtt cttcttgctc atacggattc cttatcctgc
     8161 actctggttg cggatgatga cgccggtcgt gaacaacgcg aaccccatca ccgacagaaa
     8221 gaagacgacg tcgcgggtgt cgagcacccc gcgctggaag ccctcgaagt gggtcatcac
     8281 cgaaaacgcc gcgatcacct cgaccacctg cgggctcgtg acgcgcgaca gcaggccggt
     8341 cacgggctcg tagccggcga ggatcaggaa caggcagatc aggaccgaca ggatgaagct
     8401 gatcacctgg ttgcgcgtca tggcggaggt catgcagctc accgcgaggt aggaaccggc
     8461 gagcatcagg cttcccacgt agcccgcgaa gatcacgccg ttgtccggat cgcccagcca
     8521 gttgaccgtg atcacgaccg gaaacgtcag cacgagcgcg agcgcgagga acagccagga
     8581 cgcgaggaac ttgccgacga tcgcctgcca ggtcgtgatc ggcaacgtca gcagcagctc
     8641 gagcgtgccg agccggcgct cctccgacca cagccgcatg ccgaccgcgg gcacgagaaa
     8701 gagatagagc cagggatgcc aggtgaagaa cgacaccagc gaggcctccc cgcgctcgaa
     8761 gaagctgccg accgtgaagg tgaagaagcc cgtgagcagc aggaagatca ccaggaagac
     8821 gtaggcgatg ggcgacgtga agtagccgcc cagctcgcgt ttcacgatcg ccttgatgtt
     8881 ggcccaggcg ttcatgccgc gctcctcgcc gtgtcgggca gcgtaatcgc gcggaacacg
     8941 tcctcgagcc gcccttccgg tgagcgcgcc ttcagctcct gcggcgtgcc gctggcgacg
     9001 agcctgccgc ggtcgatgat gatcacgcgc gagcaggccg cctcgacttc ctccaggata
     9061 tgggtggaga agatgatcgc cttcttctcc cccatgtgct tgatgaggtt gcgcacctcg
     9121 tgcttctgat tggggtcgag cccgtcggtt ggctcgtcca tgatcagcac gtcaggatcg
     9181 tgaatcagag attgcgcgag acaggtgcgg tgcttgtagc ccttggacaa cgtgtcaacg
     9241 ctctggtaca gcacgttctc caggaagcac agctcgacca cgcggttgac ggcttttttg
     9301 cgcgcgtcgc cctgcaggcc gcgcagctcg gcgcagaaat tgaggaagcc gtgcacggtc
     9361 atgtcggcgt agcccggcgc gttctccggc aggtaaccga acaggcgctt cgccgccagc
     9421 ggctcctcca cgatgtcgtg gccgccgacc gtgatgcgtc ccgacgtcgg cgggatgaac
     9481 cccgtcacca tgcgcatggt ggtggacttg cccgcgccgt tcggcccgag aaagcccagc
     9541 acttccccgc gctcgaccgt aaacgagacg tcgttcaccg cgagcttcgc gccgaaggcc
     9601 ttcaccaggt tctcgacttt gatcatgccc accctccggc ggaccggacc gcctgccgcg
     9661 gccgccgcct ttttttctgc gccattaaat atgaatgaac atccggattt tcaacggctg
     9721 ttcgcgctat tggaccggac tatctcaaaa ggttcccgcg gccggtgtgt taacggcctg
     9781 ttttatattc ggtttccgtt cggcaccgca caaaaaaggc ccgccgcaac gcaggccagc
     9841 tcgggtgctg gtgccgacag tctgactcga acagacgacc taccgcttac aaggcggttg
     9901 ctctaccact gagctatgcc ggcttggggc gccattatag accggataag gcgcgggccg
     9961 tgattcgtga gccggaagcc gttcgcagcc cggtccgccg gcggatcgcc acgccaatat
    10021 aatcatcggg cggaaatcat atggaaaatt cagggagggc agcatgacag agccgcgccg
    10081 gcgagtgggg gtcatcggca tcggattgat gggcacggcc tgcgcgaaac gcctgctcga
    10141 ggccgggttt gacgtgctgg gtcacgacgt cgacgcgaca aaactgaaac cgctcgaggc
    10201 gctcggaggc cgcggcgcag gctcggtctc cgaagtcgcg caggcgtgtg acagggtggt
    10261 gctcgcggtc ttcaacacgg accaggtgga ggaagcgatc gagggcccgc gcggactgct
    10321 ggccggccgc cctgcggatg cggcgcccgc tacagtaatc tgcgtcagca cctgcgaccc
    10381 cgaccgtatc gcggcgcttg cagcgcgcct gcccgccggg cgcctgcgtt tcgtcgaggc
    10441 gccggtctcc ggcaccagcg agcaggtggc gcgcggcgat gcgctgggct tgatcggcgg
    10501 cgacccggtc gcggtggaca ccgcgcgcga tgtgatcgcg gccatttgtc cgcgctggca
    10561 cgccatcggt ccggccggca gcggggggcg cgccaagctc gccatcaatc tcatactcgg
    10621 catcaaccgc gcggcactcg ccgaagggct ggtgttcgcc gagcgcatgg gactcgaccc
    10681 cgcggctttt ctcagcgtcg cgcgcgaatc cgccgcctat tcgcaaatca tggacgtgaa
    10741 gggcgggaag atgatcgccg gcgacttctc cccgcagggc tacgtcaccc agtcgctcaa
    10801 ggatttctcg ttgatgctcg cgcaagcgcg gcgcgtgaag cagcgtctgc cgctcggcga
    10861 agcctacaag gggcttatgg atggctgcat cgccgccggc gagggcgagc tcgacaacag
    10921 cgccgtcatt cgcgagatcc gccgccgcac cggctgacag gctaattcac cgccaaggaa
    10981 ggcgaacgaa ataaaactag atccgcccga tcgacattca atgcgttcgc cgcattgaat
    11041 cgaacttcgc gaccgcgagc tgagttctcg gcgtcaggat ttttattgta gatacctgta
    11101 tttcttggcg ccttcgcgcc ttggcggcta ttttatgact tgaagcttgg gcttgccgcc
    11161 gttgggcgtc gtggcgggcg gggaagtttc ggggggaggg gaaaccgaag tgccgttggg
    11221 cgccgtctcg cgcgggaaga acaggccctg cccgttttcc ttggcaaaga tgccggtcac
    11281 ggcctcgatc gggatcgagc actcgcgcga tacaccgctg aagcgggcgg agaactggat
    11341 caggtcgttg ccgatggtga gccggtgggt ggcgtcattg cccacgttca gcacgatctc
    11401 gccgttcttg acgtgctccg ccggcacccg cgtttgcgcg tccacctgaa ccgaaaggta
    11461 gggcgtgagg ccgctgtccg tgcaccactc gtggatcgca cggatgagat agggctttgt
    11521 cgacttttcc gccatcggct cagaaccccg tcactcgtca ctcgtcacaa gtcaccgtta
    11581 cttgcgcatg actttctcgg acgcggtgag cgcgtcgatg aaggccggcc ggctgaacag
    11641 gcgctcggca tacttcatca gcggcgcggc cgccttgggc agcgagatgc cgtaatggtc
    11701 gagccgccac aagagcggcg cgatcgccac gtcgagcatg gtgaattctt cgcccagcat
    11761 gtgcttctgc ttggagaaca ccggcgcgat ctgggtcagg ctgtcgcgga tgatcgcgcg
    11821 gcccttctcg gcctgcttct gcgtgccctt ctcgatcgcc tcgatgtggg cgaacatctc
    11881 gttctcgaag cggtacagga acaggcgcgc gcgcgcgcgc atcaccgggt ccgccggcat
    11941 gagctgcggg tgcggaaagc ggtcgtcgat gtactcgttg atgatgttcg actggtagag
    12001 gatgagatcg cgctccacca gcaccggcac ctggttgtag ggattcatca ccgccaggtc
    12061 ttcaggcttg ttgtagagat cgacgtcgac gatctggaag tccatcccct tctcgtacag
    12121 cacgatgcgg cagcgctggc tgaagggaca ggtggtgccg gagtatagag tcatcataaa
    12181 ttcagtgacc tgtgaccagt aatcggtgac cagaattccc cgatgggtga tgaaaaaccg
    12241 gtcactggtc accggtcacg agtcacggac gcttcagtgg atgtctttcc agtactcttt
    12301 cttcagggcg taggcgagca cgaacagcac gccgaggaac atcagcacat agaggccgag
    12361 ctgcttgcgg a
//
LOCUS       DBNGSG010000471         2366 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3209049, whole genome shotgun sequence.
ACCESSION   DBNGSG010000471 DBNGSG010000000
VERSION     DBNGSG010000471.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3209049"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..795)
                     /locus_tag="AC158Q_12510"
     CDS             complement(199..795)
                     /locus_tag="AC158Q_12510"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3340640.1"
                     /translation="MSLRLSYTLLAPFYDLVAGTAFAAARRASVAALPEDSCEQVFLN
                     GVGSGLDLPFLPRGHRYVGLDLTRAMLSRSLKRAAGIEFSAVQGNSLALPFRDASFDY
                     AILHLILAVVPDAAAALAETARVVRPGGGILIFDKFLRRGQSAPLRRLLNPLAARLVT
                     RLDVVFEDILHRVPGLRVTSDEPAAAGGWFRRIRLERL"
     gene            complement(908..1288)
                     /gene="apaG"
                     /locus_tag="AC158Q_12515"
     CDS             complement(908..1288)
                     /gene="apaG"
                     /locus_tag="AC158Q_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HIR3340641.1"
                     /translation="MADKKYDITVAARTAFIPDQSSVESSRYVFAYTITITNTGTVPA
                     QLVSRHWIITDSNNQVQEVRGDGVIGEQPLLRPNESFQYTSGTAIATPVGTMRGSYQM
                     VAQDGVQFDAPIPEFTLSMPRVLH"
     gene            1361..2029
                     /gene="rpe"
                     /locus_tag="AC158Q_12520"
     CDS             1361..2029
                     /gene="rpe"
                     /locus_tag="AC158Q_12520"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIR3340642.1"
                     /translation="MAKTYRIAPSILSADFARLGEEVRNVVAAGADLVHFDVMDNHYV
                     PNLTIGPLVCEAIRPITQALIDVHLMVKPVDRIVPDFAKAGANIISFHPEASEHVDRT
                     IALIRDQGCKAGLVLNPATPLSYLDHVIDKLDLVLVMSVNPGFGGQKFIPQALRKLAA
                     VRARIDATGREIWLEVDGGVKVDNIAEIARAGADTFVAGSAIFGTKDYKATVGAMRAE
                     LARA"
BASE COUNT          448 a          739 c          740 g          439 t
ORIGIN      
        1 tttcttcctg ttgctgctcg aagccgccgt cgcgctggcg ctgctgcttt tcatcgtgtg
       61 gtggacctgg ccgcgcaaca agccgcccgg cgggcgcgaa agcgataaga cccctcatcg
      121 ccaggaacgc aaagaaaatc aatagaaatt ctgattattg tttctctagg cggcctcggc
      181 ggttcaatcg gctgtcgctt atagtctctc caggcgtatg cgccggaacc agccgccggc
      241 agcggccggc tcgtcgctgg tcacgcgcag gcccggcacc cggtgcagga tatcctcgaa
      301 caccacatcg agccgtgtca ccaggcgggc agcgagcggg ttgagcaggc gccgcaacgg
      361 cgcgctctgc ccgcgcctca ggaatttgtc gaagatcagt attccgccac ccggccgcac
      421 gacacgcgct gtttccgcca gcgcggccgc cgcgtcggga accaccgcaa gaatcaggtg
      481 cagtatggcg taatcgaaac tcgcgtcccg gaatggtaat gcgaggctgt tgccctgcac
      541 cgctgagaac tcgatgcccg ccgcccgctt cagggagcga gagagcatgg cgcgcgtaag
      601 gtccagcccg acgtagcggt ggccgcgcgg aagaaacggc aggtcgaggc cgctgcccac
      661 gccgttgagg aacacctgct cgcagctatc ttccggcaac gccgcaacgc tggcccggcg
      721 ggctgcggcg aatgccgttc cggcgacgag atcgtagaag ggggcgagga gggtgtagct
      781 gagccgcagg ctcatcacgg gcagtgttga gtgttaagtt ttgagtgttg agttagctgc
      841 caaccggtgc tcagattgcc agaacaatcg caactcaaca ctaaacactc aaaactcacc
      901 actcacgtca gtgcaaaacg cgcggcatgg agagcgtgaa ctccggtatc ggcgcatcga
      961 actgcacgcc gtcctgcgcg accatctggt agctgccgcg catggtgccg accggcgtgg
     1021 cgatagcggt gccgctggtg tactggaagc tttcgttggg gcgaagcagc ggctgctcgc
     1081 cgatcacgcc gtctccccgt acttcctgca cctggttgtt ggagtcggtg atgatccagt
     1141 ggcggctcac cagttgcgcc ggcaccgtgc cggtgttggt gatcgtgatg gtgtaggcga
     1201 aaacatagcg gctggactcc acgctggact ggtccggaat gaaggcggtg cgcgccgcga
     1261 ccgtgatgtc gtacttcttg tccgccatgc cgccgattct tgatcatttc acggcgacgc
     1321 gcaagaacag gtagaatctc cgcggtcccg agtagaacat atggcgaaaa cctaccgcat
     1381 cgcgcccagc atcctgtcgg ccgacttcgc ccggctgggc gaggaagtgc ggaacgtggt
     1441 cgccgccggc gccgacctcg tgcatttcga cgtgatggac aaccattacg tgcccaacct
     1501 gacgatcggc ccgctggtat gcgaggcaat ccggccgatc acccaggcgc tcatcgatgt
     1561 gcacctgatg gtgaagccgg tggatcgcat cgtgccggat ttcgccaagg ccggcgccaa
     1621 catcatttcg tttcaccccg aggcctccga gcatgtcgat cgcaccatcg cgctgatcag
     1681 ggaccagggc tgcaaggcgg gtctcgtgtt gaatcccgcc acgccgctct cgtatctcga
     1741 tcacgtcatc gacaagctcg acctcgtgct ggtgatgtcg gtcaaccccg gattcggcgg
     1801 gcagaagttc attccccagg cgctgaggaa gctcgccgcc gtgcgcgccc gcatcgacgc
     1861 gaccggtcgc gagatctggc tcgaggtgga cggcggcgtg aaagtggaca acatcgccga
     1921 gatcgcgcgc gccggcgccg acaccttcgt ggcgggctcc gccattttcg gcactaaaga
     1981 ctacaaggcg accgtcggtg cgatgcgcgc cgagctggcc agggcctaag ggcattaacc
     2041 gccaagacgc caaggccgcc aagagagcca gaaaaataaa ttcaagaaag agcaggttga
     2101 gccactcgcg attcccctgt tcatttcccg gcggctcctt gcgccgggcc atcctggtaa
     2161 atggcaacga cgacccgatc ggtctacggt ttttgttctt tatcatcgaa attgattttc
     2221 ttggctgtct tggcgtcttg tcggttcgat catgaatcag cgctttccgc tgcgagtcaa
     2281 ggcagtgatg atcgacctcg acggcacgct ggcggacacc atccccgatc tcgcggccgc
     2341 ggccaacggg atgctgcatg aaatgg
//
LOCUS       DBNGSG010000472         4715 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3547836, whole genome shotgun sequence.
ACCESSION   DBNGSG010000472 DBNGSG010000000
VERSION     DBNGSG010000472.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4715)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3547836"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /gene="hrcA"
                     /locus_tag="AC158Q_12525"
     CDS             complement(<1..518)
                     /gene="hrcA"
                     /locus_tag="AC158Q_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HIR3340643.1"
                     /translation="MNERAQLLLKTLVERYITDGQPVGSRALSKYSGLDLSPASIRNV
                     MADLEELGFIASPHTSAGRIPTARGYRFFVDTLLTVKPLAAVEINQLEGQLHADNTQK
                     LVASASQLLSDLTHFAGVVMTPRRSAGFRHIEFLRLSDTRVLLILVTPEGDVQNRILS
                     AEKSYTPSELTEA"
     gene            678..1547
                     /locus_tag="AC158Q_12530"
     CDS             678..1547
                     /locus_tag="AC158Q_12530"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0019674 - NAD+ metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HIR3340644.1"
                     /translation="MKSSFKTVALIGKYKSPEVAGPLLDLARFLEGRQVKVLIDKLTA
                     SQVKQANYPVLALEDIGQKADLAIVIGGDGTMLNIARMLAPHDVALVGVNQGRLGFLT
                     DISVENMIATIGAILDGKFVTEERMLLEGQVYSGAKKTFESLAFNDVVVSKGQKGNML
                     ELEVRIDGQFIYNQRADGLIVATPTGSTAYALSSGGPIVHPSLSVMALVPVSPHTLSN
                     RPIVVSSSCTVEIVVRDNPDPRAHFDSHSRCDLREGDRIVVRRYASTLSLLHPEGHSY
                     YGMLREKLNWNRE"
     gene            1653..3326
                     /gene="recN"
                     /locus_tag="AC158Q_12535"
     CDS             1653..3326
                     /gene="recN"
                     /locus_tag="AC158Q_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIR3340645.1"
                     /translation="MLRSLSIRDFVIVDRLELEFAPGFTVLTGETGAGKSILIDALAM
                     VLGERAEAIVVRAGAERAEISAEFEADDGKAGGRALARWLRDNDLAGDDGVLLIRRVI
                     ESSGRSRGFINGRAATAAQLREAGEYLVDIHGQHQHQSLLRPAAQRGLLDAYGDLSAV
                     AEKVAVLHRAWQQRRESRMSLETNAAAFAAEREQLEWQVRELEALKFSANEWPELTAE
                     HSRLAHAASLIEAAQLGVESVSEGEGSSLAQLNAAMARLGNLVDHDPRLGEILELLES
                     ARIQLQEAAHELRRYGERLDLDPRRLQEAERRLDAIHGAARKYRVQPEELPAKLALVK
                     ERLRQMGEGGDVESLRKLEEDAHAACVSEAKQLSVGRKKAAKKLCDQVTAAMQELAMA
                     GGRFDIVLNAVTEIAAYGLENVEFLVSAHKSVAPQPLAKVASGGELSRLSLAIQTVAS
                     RVAQVPTLIFDEVDAGIGGRVAEIVGKMLRQLGTRHQVMCITHLPQVAATAEQQWQVT
                     KISTNDKVHSRVAVLDRGQRVEEIARMLGGVKITETTRKHAAEMLGIKN"
     gene            3527..4450
                     /locus_tag="AC158Q_12540"
     CDS             3527..4450
                     /locus_tag="AC158Q_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3340646.1"
                     /translation="MTLAPVAMAQEYPSKPVTFFVPFAAGSATDTLARSLGQGVTAES
                     KQNVVVDNRPGANGFIAAQAVARAAPDGYSVLIATNTTHAANEHLFKKVPYDAVKDFT
                     PVTALARGGQVMVVHPRVPAKTVKEFIALAKRQPGKLTFGSGSSSSRVASELFQQMTG
                     LQLVHVPYKSNPMAVTDLVGGHIDMMITDVVTGLPQVQAGKVRALGVSSPARLPNVPD
                     LPTIAEAGVKGYELTFWFAAYLPAKAPTAVVARLRDLFINATRAPSAQSFFKTTGIEP
                     WTTASAELAKFQASESAKWAKVIKAAGIQPE"
     gene            4554..>4715
                     /locus_tag="AC158Q_12545"
     CDS             4554..>4715
                     /locus_tag="AC158Q_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HIR3340647.1"
                     /translation="MTGTSNEKRATKNEHGGPLSGVRIIDLTTVVMGPFATQILADLG
                     ADVVKVESPD"
BASE COUNT          884 a         1426 c         1607 g          798 t
ORIGIN      
        1 gcctcggtga gctcggaggg cgtgtacgat ttctcggcgc tgaggatgcg gttttgcacg
       61 tcgccctcgg gcgtgaccag gatcagcagg acgcgcgtgt ccgaaagcct gaggaactcg
      121 atgtgccgga agcccgcgct gcgccggggc gtcatcacca cgcccgcgaa gtgcgtaaga
      181 tcggagagca gctgcgacgc ggaagccacg agcttttgcg tgttgtccgc gtgcagctgc
      241 ccctcgagct ggttgatctc gaccgcggca agcggtttta ccgtgagcag ggtatccacg
      301 aagaagcgat agccgcgcgc ggtcggtatc cgcccggcgg aggtgtgggg gctcgcgatg
      361 aaacccagct cctcgaggtc ggccatcacg ttgcggatgc tcgcgggcga gaggtccagc
      421 ccggagtact tggacagcgc ccgggagccg accggctggc cgtccgtgat gtagcgctcg
      481 accagtgttt tcagcagcag ctgcgctcgc tcgttcaatg cgggggtgtc ggcgggcatg
      541 gcctattcta ctaaaaaccg tgaatgatga atggtgaatc gtgaatcgaa aaacccgctg
      601 tttccgggcc atttgccagt cggcgaatca cgattcacca atcacgactc acgggggttt
      661 tatggtttaa tgcgggcatg aaatcgtcat tcaagaccgt cgcgctgatc ggcaagtaca
      721 agagcccgga agtcgcgggg ccgctgctcg acctggcccg cttcctggag ggccggcagg
      781 tgaaggtcct gatcgacaag ctgacggcgt cccaggtgaa gcaggccaat taccccgtcc
      841 tggcgcttga ggacatcggc cagaaggccg acctcgccat cgtcatcggc ggcgatggca
      901 cgatgctcaa tatcgcgcgc atgctcgcgc cccacgatgt cgcgctggtc ggggtcaacc
      961 aggggcgact gggctttctg accgacatct ctgtggaaaa catgatcgcg accatcggcg
     1021 ccatccttga cgggaagttc gtcactgagg agcgcatgct gctcgaaggt caggtctaca
     1081 gcggcgcaaa aaagacgttc gagtcgctgg ctttcaatga tgtggtagtg agcaaggggc
     1141 aaaagggcaa catgctggag ctggaggtgc gcatcgacgg ccagttcatc tacaaccagc
     1201 gcgccgacgg cctgatcgtc gccaccccga ccggctccac cgcctacgct ctgtcctcgg
     1261 gggggcctat cgtgcatccg tcgctctcgg ttatggcgct ggtgccggtg tccccccaca
     1321 cgctgtccaa ccgcccgatc gtggtgagca gcagttgcac ggtggagatc gtcgtgcgcg
     1381 acaatcccga cccgcgcgcg catttcgaca gccactcgcg ctgcgacctg cgcgaaggcg
     1441 accgcatcgt ggtgcggcgc tacgcgagca cgctcagcct gctgcatccg gagggacaca
     1501 gctactacgg catgctgcgg gagaagctca actggaacag agagtaaggc ttgaatcgtg
     1561 aatcgtgagt cgtgaatcga gaaaccccga tcttcagggc cgcccgagca cactcgaatc
     1621 acgattcacg aatcacaaat cacgctttca tcatgttgcg ttcgctttcc atccgcgact
     1681 tcgtcatcgt cgaccggctc gagctcgagt tcgcgccggg tttcacggtg ctgacgggcg
     1741 agaccggagc cgggaaatcc atacttatcg acgcgctggc gatggtgctg ggcgagcggg
     1801 cggaggcaat cgtggtgcgc gccggcgcgg agcgcgccga aatcagcgcg gagttcgagg
     1861 ccgatgacgg caaggcgggc ggcagggcgc tggcccgctg gctcagggac aacgatctcg
     1921 ccggcgacga cggcgtgttg ctgatacggc gcgtgatcga gtcctccgga cgctcgcgcg
     1981 gcttcatcaa tggtcgcgcg gccacggccg ctcaattgcg cgaggccggg gagtacctgg
     2041 tggatatcca cggccagcac cagcaccagt cgctactgcg gccggcggcc cagcgcgggc
     2101 tgctcgatgc ctacggcgat ctttcggctg ttgccgaaaa ggtggccgtg ctccatcgcg
     2161 cgtggcagca gcggcgcgag agccgcatgt ccctggaaac caatgcagcc gcgttcgccg
     2221 cggaacgcga acagctcgag tggcaggtgc gcgaactcga ggcgctcaag ttcagcgcga
     2281 acgaatggcc ggagctgacg gccgagcatt cgcggcttgc gcatgcggcc agcctgatcg
     2341 aggccgccca gcttggcgtg gagtccgtct ccgaaggcga gggctcgagt ctcgcacaac
     2401 tgaatgcggc catggccagg ctcggcaatc tggtcgacca tgatccccgg ctcggagaga
     2461 tcctcgagtt gctcgagtcg gcaagaatcc agctgcagga agcggcgcac gagctgcgcc
     2521 gctacgggga gcgcctcgac ctcgatccgc ggcgcctgca ggaggccgag cggcggctgg
     2581 atgccataca cggcgctgcg cgcaagtacc gggtgcagcc cgaggagctg ccggcaaagc
     2641 tggcgctcgt gaaagagcgg ctgcggcaaa tgggggaggg cggcgacgtg gagtcgttgc
     2701 gcaagctgga agaagatgcg catgcggcgt gcgtctccga agcaaagcag ttgtcggtcg
     2761 gcaggaaaaa agccgcgaag aagctctgcg accaggtgac cgccgccatg caggaactcg
     2821 cgatggcagg agggcggttc gacattgtgc tcaatgcggt gaccgagatc gcggcatacg
     2881 ggctggaaaa cgtcgaattc ctcgtctccg cgcacaagag cgtggccccg cagccgctgg
     2941 cgaaggtggc ttccggcggc gagctgtccc gtctgtccct ggcgatccag accgtggcga
     3001 gccgggtcgc gcaggtgccg acgctgattt tcgacgaggt ggacgcaggc atcggcgggc
     3061 gcgtcgccga gatcgtcggc aagatgctga ggcagctcgg aacccggcac caggtgatgt
     3121 gcattaccca tctgccgcag gttgcggcga cggccgagca gcaatggcag gtgaccaaga
     3181 tcagcacgaa cgataaggtg cattcccgcg tggctgtgct ggacaggggg cagcgcgtgg
     3241 aggagattgc gcggatgctg ggcggggtga agatcaccga gacgacgcgc aagcacgcgg
     3301 cggagatgct ggggattaag aattagccgc caagttgcgt taaatccgga cgcttggcgc
     3361 cggtttaact tcgcgtgttg gaacggacga actaatcgcc gttcaacacg cggctgccaa
     3421 gtggcgagac aatgagttgc cagtcatgct tactcagata ttggaggaag aagccatgtt
     3481 tcatcaatac aggttccctg ttaatttgat cccattcatc gcggcgatga cgctagcgcc
     3541 cgtcgcaatg gcgcaggagt acccgtcgaa gccggtgacg ttcttcgttc cgtttgcggc
     3601 ggggagcgcg accgacacgc tggctcgctc gctggggcag ggggtcaccg ccgagtcgaa
     3661 gcaaaacgtg gtggtcgaca atcggcccgg tgcgaacggg ttcatcgcgg cccaggcggt
     3721 cgcgagggcg gcgccggacg gctattcggt gctgatcgcg accaacacga cccacgccgc
     3781 gaacgagcat ctcttcaaga aagtgccgta tgacgcggtg aaggatttta cgcctgtgac
     3841 cgcgcttgcg cgcggcgggc aggtcatggt cgtgcacccg cgcgtcccgg cgaagaccgt
     3901 caaggaattc atcgcgctgg cgaagcgcca gccgggcaag ctgactttcg gcagcggcag
     3961 ctcctcgagc cgcgtcgcca gcgaactgtt ccagcaaatg acggggctgc agttggtgca
     4021 cgtgccctac aagagcaacc cgatggccgt taccgacctc gttggcggcc atatcgacat
     4081 gatgatcacc gacgtcgtca ccggactgcc gcaggtgcag gccggcaagg tgcgcgcgct
     4141 gggcgtgagc agcccggcac gcctgcccaa cgttcccgac ctgccgacga tcgccgaggc
     4201 cggcgtgaag ggttacgagc tgaccttctg gttcgcggcc tacctgccgg cgaaagcgcc
     4261 gaccgcggtc gttgcgcggc tgcgcgacct gttcatcaac gctacgcggg cgccgtccgc
     4321 gcagtcgttc ttcaagacta ccgggatcga gccctggacc accgcttccg cggagctcgc
     4381 gaagttccag gcatcggagt cggccaagtg ggccaaggtc atcaaggccg ccggcataca
     4441 acctgaatga aacgagtgac tcgttactcg tttctcgttt ctggtgggcc gagtcgcttc
     4501 gcgtggcgcg acttacggcg ctgatgcaga ttacataact cggtttctat tgaatgaccg
     4561 gcacgagcaa cgagaaacga gcaacgaaaa acgagcacgg cgggccgctc tccggcgtgc
     4621 gcatcatcga cctcaccacg gtggtgatgg ggccgttcgc gacgcagatc ctcgccgacc
     4681 tgggggccga tgtcgtgaag gtcgagtcgc cggac
//
LOCUS       DBNGSG010000473         4037 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_152915, whole genome shotgun sequence.
ACCESSION   DBNGSG010000473 DBNGSG010000000
VERSION     DBNGSG010000473.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4037)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_152915"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2853
                     /locus_tag="AC158Q_12550"
     CDS             <1..2853
                     /locus_tag="AC158Q_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HIR3340648.1"
                     /translation="TDASIYQIEPVGVVVPRSEVDVLAALAVAREEGVPVLPRGGGTS
                     QCGQTVGAALVIDHSKHLNRILGFDPKARTVTVQPGVVLDQLNAWLKPHGLWYPVDVS
                     TSAQATLGGMAGNNSCGSRSIQYGNMVHNVRGIDAVLPDGSEYAFGEVKGETEGPAGY
                     RELVKKIRAIAAREAEEIEHRYPKLLRRVGGYNLDMISGIPFNTAHLLVGSEGTLAYS
                     RRIHLDLSPLPKHKTLGVCHFPTFYQAMEAPQRIVKLGPAAVELVDRTMIGLARGNPA
                     FRATVDQCVKGDPDAILLVEFAGDERDEQLRRLKQLTELMGDLGFPGGVVEVVEPGLQ
                     RDLWEVRKAGLNIMMSMKGDGKPVSFIEDCAVPLEHLAEYTDRLTRVFEKHGTRGTWY
                     AHASVGCLHVRPVLDMRREGAAQMRAIAEEAAALVKQYKGAYSGEHGDGLVRSEWIEP
                     FFGPKLTRAFGEIKDLFDPKGLLNPGKIVRPSKQDDRSLFRYKPGYAVNPIRTALDWS
                     EHGGLDKAAEMCNNNGHCRKFDAGTMCPSYRVTREEQHLTRGRANTLRLALSGQLAVK
                     EGEDALASGELYATFDLCVSCKGCKRECPTGVDMAKMKIEFLHHYRQRHALSAREKLI
                     AYLPRYAPLVSRFSFLVNAMQGAGKSFLGFAPQRSLPKWRGDAFRDAAPHPNTLPAAG
                     ERGPIESPRPQSGERDRVRGKTEVVLLVDTFSRYFEPENARAAVAVLRAAGCTVHLPR
                     PADGSRPLCCGRTFLAAGLVDEAKLEARRTIETLKPYVERGVPVVGLEPSCLLGLRDE
                     FLSMLPGADSAALGLRAFLFEEFLAREHEAGRLHLKLGPLREKKALLHGHCHQKAFAA
                     MPDVERVLKLVPGLAVETIESSCCGMAGAFGYEAAHYDVSMKMGELALLPRVRKAASD
                     ELIVADGTSCRHQIHDGAQREAMHVARVLERALNGG"
     gene            2924..3442
                     /locus_tag="AC158Q_12555"
     CDS             2924..3442
                     /locus_tag="AC158Q_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011660863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR3340649.1"
                     /translation="MPLILRALEFAAHKHRDQRRKDKRASPYINHPIQLASVLVREGG
                     VTDPAVIAAALLHDTIEDTETALQELRGEFGDEVADVVLEVTDTKWIRKAARKRLQVA
                     RARHASDGAKLVKLADKIVNLRDVAAHPPAGWSLERRREYFDWAKEVIDRMRGTHPEL
                     ERRFDEAYALKP"
     gene            complement(3532..>4037)
                     /locus_tag="AC158Q_12560"
     CDS             complement(3532..>4037)
                     /locus_tag="AC158Q_12560"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TauD/TfdA dioxygenase family protein"
                     /protein_id="HIR3340650.1"
                     /translation="VQVNGGGDTSFNNPYLAYEELPEDLKRAIEGKYQVHDTSRNSAG
                     VLRPGVKLPTKPEEVEGPTHPLVRVHPVTGRRALYLGRRRVWPSNYIVGIPNEDSERL
                     LDRLWAHATQPKYAWTHKWQVGDVILWDNRCCMHYRTEVDTAQRRVMHRTTIKGEPPV
                     APWETAA"
BASE COUNT          672 a         1333 c         1392 g          640 t
ORIGIN      
        1 acggacgcct cgatctacca gatcgagccc gtaggggtcg tggtgccgcg gagcgaggtg
       61 gacgtgctcg ccgccctcgc ggtcgcgcga gaggagggcg tgccggtcct gccgcggggc
      121 ggcggcacct cgcagtgcgg ccagaccgtc ggcgccgcgc tggtcatcga tcactcgaag
      181 cacctcaatc ggatcctcgg cttcgacccg aaggcacgca cggtgaccgt gcagcccggt
      241 gtcgtgctcg atcagttgaa tgcctggctc aagccgcacg gcctgtggta cccggtggat
      301 gtctcgacca gcgcgcaggc gacgctgggc ggcatggcgg gcaacaactc ctgcggctcg
      361 cgctccattc aatacggcaa catggtgcac aacgtgcgcg ggatcgacgc cgtgcttccc
      421 gacggctccg agtacgcgtt cggcgaggtg aagggtgaaa ccgaaggccc cgccggctac
      481 cgggaactgg tgaagaagat ccgcgccatc gccgcgcgcg aggcggagga gatcgagcac
      541 cgctatccga aactgctgcg ccgtgtcggc ggctacaacc tggacatgat ttccggcatc
      601 cccttcaaca cggcgcacct gctggtaggc tcggagggca cgctcgccta ctcgcgccgc
      661 atccatctcg atctttcccc gctgccgaag cacaagaccc tcggcgtgtg ccacttcccg
      721 acgttctacc aggcgatgga agcgccgcag cgcatcgtga aactcgggcc cgccgcggtg
      781 gaactggtgg accgcaccat gatcggcctc gcgcgcggca acccggcgtt tcgcgccacg
      841 gtggaccagt gcgtcaaggg cgatccggac gcgatcctgc tggtcgaatt cgccggcgat
      901 gagcgcgacg aacagttgcg gcgcctgaag caactcaccg agctgatggg cgacctggga
      961 ttcccgggcg gcgtggtcga agtcgtcgag cccggcctgc agcgcgacct ctgggaagtg
     1021 cgcaaggcgg gactgaacat catgatgtcc atgaagggcg acggcaagcc ggtgtccttc
     1081 atcgaggact gcgcggtgcc gctcgagcac ctcgccgagt acaccgaccg cctgacgcgc
     1141 gtgttcgaga agcacggcac gcgcggcacg tggtacgcgc acgcctcggt cggctgcctg
     1201 cacgtgcggc cggtgctcga catgcggcgc gagggcgcgg cgcagatgcg cgccatcgcc
     1261 gaggaggccg cggccctggt gaagcagtac aagggggcgt attccggcga gcacggcgac
     1321 ggcctggtgc gctccgagtg gatcgagccg ttcttcgggc ctaaattgac gcgcgcgttc
     1381 ggagaaatca aggatctctt cgatccgaag gggctcctca accccggcaa gatcgtgcgg
     1441 ccgtcgaagc aggacgatcg ctcgctgttt cgctacaagc cgggctatgc ggtgaacccg
     1501 atcaggaccg cgctcgactg gtccgagcac ggcggcctcg ataaggcggc cgagatgtgc
     1561 aacaacaacg ggcattgccg caagttcgac gccggcacca tgtgtccgag ctaccgcgtc
     1621 acgcgcgagg agcagcatct cacgcgcggg cgcgccaata cgctgcggct cgcgctgtcg
     1681 ggacagctgg ccgtaaagga aggcgaggat gcgcttgcat cgggggagtt gtacgccacg
     1741 ttcgacctgt gcgtctcgtg caagggctgc aagcgcgaat gcccgaccgg cgtggacatg
     1801 gcgaagatga agatcgaatt tctccaccac tatcgacaac gccatgcgtt gtcggccagg
     1861 gaaaaactga tcgcttactt gccgcgctac gcaccgctcg tttctcgttt ctcgtttctc
     1921 gtcaacgcaa tgcagggcgc ggggaagagc tttctcggct ttgcgccgca gcgttctttg
     1981 ccgaagtggc gcggcgatgc gttcagggac gccgcccctc accccaacac tctccccgca
     2041 gccggggaga gggggcccat tgaatcccct cgcccccagt cgggggagag ggacagggtg
     2101 agggggaaga ccgaggtcgt gctgctcgtg gacaccttca gccgctactt cgagccggag
     2161 aatgcccggg ccgcggtcgc cgtgttacgg gccgcgggct gcaccgttca tctcccgcga
     2221 cccgcggacg gctcgcgtcc gctgtgttgc ggccgcacgt tcctcgcggc cggcctggtg
     2281 gacgaagcga aactcgaggc gcggcgcacg atcgagacgc tgaagcccta cgtcgagcgc
     2341 ggcgtgccgg tggtggggct ggagccgtcc tgcctgctcg gactgcgcga cgagttcctg
     2401 tcgatgctgc ccggcgcgga ctcggcggcg ctcggcctgc gcgcattcct gttcgaggaa
     2461 tttctcgcgc gcgagcacga ggcggggcgc ctgcacctga aactcgggcc gttgcgggag
     2521 aagaaggcgc tgctccacgg ccattgccac cagaaggcct ttgccgcgat gcccgacgtc
     2581 gagcgcgtgc tgaagctcgt ccccggcctc gcggtggaga cgatcgagtc gagctgctgc
     2641 ggcatggccg gcgccttcgg ctatgaagcg gcccattacg acgtctccat gaagatgggc
     2701 gagctcgcgc ttctgccgcg cgtgcgcaag gcggcgagcg acgaattgat cgttgccgac
     2761 ggcaccagct gccgccacca gatccacgat ggagcgcagc gcgaggcgat gcacgtggcc
     2821 cgggtgctgg agcgggcatt gaacggtgga tagccggacc gtgtcgaaac acgaaacacg
     2881 aatcacgacc caccctgcag cggatgcggc gacggccacc ggcctgccgc tgattctcag
     2941 ggcgctggaa ttcgccgcgc acaagcaccg cgaccagcgc cgcaaggaca agcgcgcctc
     3001 cccttacatc aatcacccga tccagctcgc cagcgtgctc gtgcgcgaag gcggggtgac
     3061 cgacccggcg gtgatcgcgg cggcgttgct tcacgacacc atcgaggata ccgagaccgc
     3121 cctgcaggag ctgcgcggcg agttcggcga cgaggtcgcc gacgtggtgc tggaggtgac
     3181 cgacaccaag tggatccgga aggcggcgcg caaacggctg caggtggcgc gcgcgcgcca
     3241 cgccagcgac ggcgccaagc tggtcaagct tgccgacaag atcgtcaacc tgcgcgacgt
     3301 cgccgcgcat ccgccggcgg ggtggtcgct cgagcgccgc cgcgagtact tcgactgggc
     3361 gaaagaggtc atagaccgga tgcgcggcac gcacccggag ctcgagcgcc gcttcgacga
     3421 ggcttacgcc ctcaagccct gacgggcgaa gttacaagtc acaagttgca aggaccaaga
     3481 ccagcgatct tgtaacttga aacttgtaac ttgaaacttg taccggcggc gtcaagccgc
     3541 cgtttcccaa ggagcgaccg gcggctctcc cttgatcgtc gtgcggtgca tcacgcgccg
     3601 ctgcgcggtg tccacttccg tgcggtagtg catgcagcag cggttgtccc acaggatgac
     3661 gtcgccgacc tgccacttgt gggtccaggc gtacttcggt tgcgtcgcgt gcgcccacag
     3721 ccggtcgagc aggcgctcgc tgtcctcgtt cggtatgccg acgatgtagt tggacggcca
     3781 cacgcggcgc cggccgaggt acagcgcgcg ccggccggtc accgggtgca cgcgcaccag
     3841 cgggtgcgtg gggccctcga cttcctcggg cttcgtcggc agcttcacgc cggggcgcag
     3901 cacgcccgcg ctgttgcggc tggtgtcatg cacctggtac ttgccttcga tcgcgcgctt
     3961 gaggtcctcc ggcagctcct cgtaggcgag gtacgggttg ttgaaggagg tgtcgccgcc
     4021 gccgttcacc tgcactt
//
LOCUS       DBNGSG010000474         5512 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3548425, whole genome shotgun sequence.
ACCESSION   DBNGSG010000474 DBNGSG010000000
VERSION     DBNGSG010000474.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5512)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5512)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5512
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3548425"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /locus_tag="AC158Q_12565"
     CDS             complement(<1..1202)
                     /locus_tag="AC158Q_12565"
                     /inference="COORDINATES: protein motif:HMM:NF025052.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside
                     phosphotransferase/ATP-binding protein"
                     /protein_id="HIR3340651.1"
                     /translation="MNAPAARRAAAGTPLFVALTRPECYPHPVAKVRLIETHISWVML
                     TGPFAYKIKKPVNLGFADFSTLALRRHYCEEELRLNHRFAPDLYLDVVPIRGDPRAPR
                     IGGDGPLLDYAVRMREFSQDALAARALAAGTFGARELDTLAARVAEFHAAAPAAQAGG
                     GLGHPASVLGSARQNFEQMLPLARSARDNRALRALQRWTDCEYAARQQAFASRQSGGF
                     VRECHGDLHLGNIVMLDGRPVPFDCIEFNDELRWIDVMNETAFLAMDLEDRGRDDLAW
                     RFLNRYLEATGDYEGIGVLRFYLAYRALVRAKVHLMRSRQPRVGRAEKARLARAFHGY
                     LRLAGRYAAPARGALVIAHGLSGSGKSTATQPLIEGLGAIRLRSDLERKRLHGLAPLA
                     VSGSGPGRG"
     gene            1344..1739
                     /locus_tag="AC158Q_12570"
     CDS             1344..1739
                     /locus_tag="AC158Q_12570"
                     /inference="COORDINATES: protein motif:HMM:NF013427.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HIR3340652.1"
                     /translation="MTYLSQEQTTQIGQLLRERRRVLRNEIREGLLKSGEAHHKDFAG
                     MVSDAGDESVANMLEDLEIAEVERDVRELREVENALARISRGDFGACADCGEPIGFPR
                     LKANPAAVRCRPCQERRERGSAHERTPTL"
     gene            complement(1864..2154)
                     /locus_tag="AC158Q_12575"
     CDS             complement(1864..2154)
                     /locus_tag="AC158Q_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ElaB/YgaM/YqjD family protein"
                     /protein_id="HIR3340653.1"
                     /translation="MNAPTEKIVSDARILVTDVEELLKATATQTGEKVASARARVQAG
                     LVEARDTVVLQTRHAAQATDRYVHENPWQAAGISAGVSVGIGLLIGLLLGRR"
     gene            complement(2250..2447)
                     /locus_tag="AC158Q_12580"
     CDS             complement(2250..2447)
                     /locus_tag="AC158Q_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HIR3340654.1"
                     /translation="MPWNEQRYPKSMKRLLPRVRRKAIRIANALLKEGYDEGRCIRIA
                     ISRARRWGSGQAGAAGSRAHP"
     gene            complement(2457..2918)
                     /locus_tag="AC158Q_12585"
     CDS             complement(2457..2918)
                     /locus_tag="AC158Q_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007827769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3340655.1"
                     /translation="MFKRILVPVDGSRTSWLGLDKAIRLAREQRATLCLLHVVDERVT
                     TQYFEGGAAAIDRLLDSLLEGGKRVLARAEAKARKQRVRVKTVLVENVIRSVADVIVG
                     QARKWRADLIVIGTHGRRGVSRLVMGSDAEGVVRTTPVPVLLVRSKAGRQR"
     gene            3084..4325
                     /locus_tag="AC158Q_12590"
     CDS             3084..4325
                     /locus_tag="AC158Q_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3340656.1"
                     /translation="MAADENSPLIRSPWLKRGLLIALALAAAGFALRNVILGAPVETV
                     RAVRSDLAQTVVASGRIMTPQRVSVGAVITGRVARIPVQEGQSVRSGDVLMELDDSDE
                     RAAVEQSRAAVAQAEAKLRQLRELALPAAEQGLVQAQANLTQSRRQYDRAVELKAKGF
                     VSQAALDDAQRNLDVAESQLRSAKLQVQSNSRAGSEFAVAETTLAQARAGLHVAQAKL
                     DETIIRAPVGGILIARAVEPGNVVQPGKELMALAPSGETQVVVQIDEKNLAQLRVGQN
                     ALGSADAYPRERFAAELVYINPGIDALRGAVEVKLRVPNPPGYLRQDMTVSVDIEVAR
                     SAGAVVVPADAVRDANGAQPWVLAVDGGRATRRAVKLGLKGDGRMEVLEGVAPGDLLV
                     SAAQQAVKAGQRVRAIAPVRP"
     gene            4322..>5512
                     /locus_tag="AC158Q_12595"
     CDS             4322..>5512
                     /locus_tag="AC158Q_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3340657.1"
                     /translation="MNRLAFFEWIVAFRFMRDGLMQTLLIILGVGLGGGVIIFMSAVL
                     SGLQDNVVRRTLNFQAPIVILPPDQVARPLRADARAEVAAQVQPRSQQLRSIDQWQKV
                     REQVARTPGVIALTPVVSGPGFALRGDANKAVTITGIEQETYFDVIALPEKIVAGRHQ
                     VGPLDIIIGTELSADLGSDVGDKLRLTTASGASATLTVTGIFDFGNKSVNERSVYIAL
                     RTAQSLLDLAGGATAIEVKVLDPFAAETVAQVLRAGTDLQVDSWIRTNAQFFTAMAAQ
                     IVTNTVIRVFVGLTVALGIASVLVVSVIQKSREIGILRAMGTSRAQVLRVFLIQGGAM
                     GLAGSVLGSLLAWGFLLVWRAVARNPDGTPMFVIQMEPSLFAIATAGATLVGILAAVA
                     PARRA"
BASE COUNT          904 a         1787 c         1912 g          909 t
ORIGIN      
        1 ccgcgcccgg gaccggagcc gctcaccgca aggggggcga gaccgtggag gcgcttgcgc
       61 tcgaggtcgg agcgcaggcg aattgcgccc aaaccctcga tcagcggctg tgtcgcggtg
      121 gacttgccgc tgccggaaag gccgtgagcg atgacgagcg ccccgcgcgc cggcgcggcg
      181 tagcgcccgg caagccgcag gtagccgtga aaggcgcgcg ccaggcgggc cttttccgcg
      241 cgacccaccc gcggctggcg cgagcgcatg aggtgcacct tggcgcgcac cagcgcccgg
      301 tacgcgagat agaagcgcag caccccgatg ccctcgtaat cgccggtcgc ttcgaggtag
      361 cggttcagga agcgccatgc gagatcatcg cggccgcggt cctcgaggtc catcgccagg
      421 aatgcggtct cgttcatcac gtcgatccag cgcagctcgt cgttgaactc gatgcagtcg
      481 aacggcactg gccggccgtc gagcatcacg atattgccca agtgcaggtc gccgtggcac
      541 tcgcgcacga acccgcccga ttgccgggac gcaaaagcct gttgccgggc ggcatattca
      601 caatcggtcc accgctgaag ggcacgcagg gcgcgattgt cccgggcgct cctggcgagc
      661 ggcagcatct gctcgaaatt ctgccgcgcg gagccgagca ccgaggcagg atggcccagg
      721 ccgccgcccg cctgcgccgc cggtgcggcg gcgtgaaatt ccgcgacccg ggcggcgagg
      781 gtatcgagtt cgcgcgcgcc gaatgtgcct gctgcgagcg cgcgggcggc cagagcatcc
      841 tgggagaact cgcgcattcg caccgcataa tcgagcagcg ggccgtcgcc gccgatgcgg
      901 ggcgcacggg gatcaccccg tatcggcacg acgtccaggt acaggtccgg cgcgaagcgg
      961 tggttcaggc gcaattcctc ctcgcagtaa tgccgccgca gggcgagcgt ggagaaatcc
     1021 gcgaaaccga gattgaccgg cttcttgatc ttgtacgcga acgggccggt gagcattacc
     1081 caggagatgt gcgtttcgat caaacgcact tttgcgaccg ggtggggata gcactccggg
     1141 cgcgtgagcg ccacgaagag gggcgtcccg gcggcggcgc gccgcgcggc gggtgcgttc
     1201 atggcgggtg gagtcctctt gccgaatttg accgcaatgc ccgcgttctc cgcttgatct
     1261 gcatcaatcg ggatggcgca ggcccggata gcatgatgaa tcggcgggcg tgtcccgcgc
     1321 aaccagacga aaacaaggtc ccaatgacct atctaagcca ggagcagacc acgcagatcg
     1381 ggcagttgct gcgcgagcgc cggcgcgtac ttcgaaacga gatccgggaa ggcctgctga
     1441 agtcgggcga agcgcatcac aaggatttcg ccggcatggt gtccgacgcc ggggacgaat
     1501 ccgtcgcgaa catgctggag gacctggaga ttgccgaggt cgaacgcgat gtgcgcgaac
     1561 tgcgggaagt cgagaatgcg ctggcgcgga tcagtcgcgg tgatttcggc gcctgcgccg
     1621 attgcggcga acccataggt tttccgcgcc tgaaggccaa tcccgctgcg gtgcgctgcc
     1681 gcccgtgcca ggaacggcgc gagcgcggtt ccgcgcatga gcgcaccccg acgctctagc
     1741 gacgacgtgt aaggcacaag gcacaaggta gaaggaacaa ggtacgacgc caaccgtcct
     1801 accttgaacc ttgaaccttg aaccttgaac cttgaacctt gaaccttgaa ccttgctact
     1861 gtttcaacgc cggccgagca gcaggccgat gagcaacccg ataccgacgg acacgcccgc
     1921 ggagattccc gcggcctgcc acggattttc gtggacgtag cggtcggttg cctgggcggc
     1981 gtgtctggtc tgcagcacga cggtatcccg ggcttccacc aggccggcct gcacgcgagc
     2041 acgcgcactg gcgactttct cgcctgtctg cgtggcagtg gctttgagca attcctcgac
     2101 atcggtgacc aggattcggg catcgctgac gatcttttcc gtgggggcat tcatgtttga
     2161 ctcctgtcag gttgatgagc accgacccag cgcatgggtg cggcatgtaa atagtctatc
     2221 gagccggacc ggccgcactt tgacctggat caaggatgcg ctctcgagcc ggcggctccc
     2281 gcttgaccgg agccccagcg cctcgcgcgc gagatcgcga tgcggatgca gcggccctcg
     2341 tcatagcctt ccttcagcag ggcgttggcg atcctgatcg ccttgcgccg cacgcgcggc
     2401 agcaggcgtt tcatcgactt cgggtagcgc tgctcgttcc agggcatgat cgacgtttag
     2461 cgctgtctgc ctgctttgga cctgaccagg agcaccggga cgggggtcgt gcggacgacg
     2521 ccttcggcat cgctgcccat caccaggcgg gagacgccgc ggcggccgtg ggtgccgatg
     2581 acgatgagat cggcgcgcca tttcctcgcc tgtcccacga tcacgtcggc cacgctgcgg
     2641 atcacgtttt cgaccagcac ggttttcacc cggacgcgct gcttgcgggc ctttgcctcg
     2701 gccctggcga ggacccgctt gccgccctcg agtagggaat cgagcagccg gtcgatggcg
     2761 gccgcgccgc cctcgaagta ctgcgtggtc actcgctcgt ctaccacgtg cagcaggcac
     2821 agcgtggcgc gctgttcccg tgccagccgg attgccttgt cgaggccgag ccacgatgta
     2881 cgactgccgt ccaccggcac caggattcgt ttgaacatgt catgctcctt tcgatcgtgg
     2941 aagtgtgtca cggtccggaa ccgcggatca ggatcccagt ctattcatga ccggagcgcg
     3001 cagtttgata tggatcaagc gacagcccgg cccgcaacgg ctatgcttcg accttcgaac
     3061 agtgaggatg tgccgttgat ttcatggccg cagacgaaaa ttccccgttg atccgcagtc
     3121 cctggctgaa gcgcgggttg ctgattgcgc ttgccctggc agcggcgggg ttcgccctgc
     3181 gcaacgtcat cctgggcgcg ccggtcgaga ctgtccgggc cgtccgcagc gatctcgccc
     3241 agaccgtggt ggcgagcggc aggatcatga ctccgcaacg cgtttccgtg ggcgcggtaa
     3301 ttaccggtcg cgtcgcgcgc atcccggtgc aggaaggcca gagcgtccgc agcggcgacg
     3361 tgctgatgga actggacgac agtgacgagc gcgcggccgt ggaacaatcc cgggccgcgg
     3421 tcgcgcaggc cgaggcgaag ctgcgccagc tgcgcgagct ggcgttgccc gcggccgagc
     3481 agggcctggt gcaggctcag gcaaacctca cccagtcgcg ccggcaatac gacagggcag
     3541 tcgagctcaa ggccaaggga ttcgtgagcc aggcggcgct ggatgatgcg cagcgcaacc
     3601 tcgacgtggc ggagagccag ctgcgctccg cgaaattgca ggtccagtcg aacagccggg
     3661 cgggcagcga gttcgcggtg gcggaaacca cgctggcgca ggcacgcgcg ggcctgcacg
     3721 tggcgcaggc gaagctcgat gagaccatca tccgcgcgcc cgtcggcggc atcctgatcg
     3781 cgcgcgccgt cgagcccggc aacgtcgtgc agcccggcaa ggagttgatg gcgctcgcgc
     3841 cgtccggcga aacccaggtg gtggtgcaga tcgacgagaa gaacctggcg cagctgaggg
     3901 tggggcagaa cgcgctcggt tctgccgacg cctatccgcg cgagcgtttc gcggcggagc
     3961 tcgtctacat caatcccggc atcgacgcgc ttcgcggcgc ggtggaagtg aagctgaggg
     4021 tgccgaatcc gcccggctac ctgcgacagg acatgacggt ctcggtcgac atcgaggtcg
     4081 ctcgcagtgc tggcgccgtg gtggtgcccg ccgatgcggt gcgcgatgcg aacggcgcgc
     4141 aaccctgggt gctcgccgtg gacggcgggc gcgccacgcg ccgcgcggtg aagctggggc
     4201 tgaagggcga cgggcgcatg gaagtgctgg agggcgtggc acccggggac cttctcgttt
     4261 ccgcggccca gcaggcggtc aaggccgggc agcgcgtgcg cgcaatcgca ccggtccggc
     4321 cgtgaatcgc ctcgcgtttt tcgagtggat cgtcgccttc cgcttcatgc gtgacggcct
     4381 gatgcaaacg ctgctcatta ttcttggcgt cgggctcggc ggcggcgtga tcatattcat
     4441 gtcggccgtg ctgtcgggtc tgcaggacaa cgtcgtgcgg cgcacgctca acttccaggc
     4501 gcccatcgtc attcttccgc ccgaccaggt ggcgcgcccg ctgcgcgccg atgcgcgcgc
     4561 cgaggtggcg gcccaggtcc agccgagatc gcagcaattg cgctccatcg atcaatggca
     4621 gaaggtgcgc gagcaggtgg cgcgtacgcc cggggtgatt gccctgacgc ccgtcgtctc
     4681 gggccccggc ttcgccctgc gcggggacgc gaacaaggcc gtcacgatca cgggcatcga
     4741 acaggaaacc tacttcgacg tcatcgcgct gccggagaaa atcgttgccg gacgccatca
     4801 ggtcggaccg ctcgacatca tcatcggcac cgagctgtcc gccgatctcg gctctgacgt
     4861 cggcgacaag ctgaggctga cgacggcctc gggcgcatcc gcaacgctga cggtcaccgg
     4921 catcttcgat ttcggcaaca agagcgtgaa cgagcgcagc gtctatatcg cgctgcgcac
     4981 ggcgcagagc ctgctcgacc tggcgggcgg cgccaccgcc atcgaggtca aggtgctgga
     5041 cccgtttgct gccgagaccg tcgcgcaggt tctccgcgcc ggcaccgatc tccaggtgga
     5101 cagctggatc cggaccaatg cgcagttctt caccgcgatg gcggcgcaga tcgtcaccaa
     5161 taccgtcatc cgcgtgttcg tgggcctgac ggtcgcgctc ggcatcgcca gcgtgctggt
     5221 ggtctcggtc atacagaaat cgagggagat cggcatcctt cgggccatgg gaacgtcgcg
     5281 cgcgcaggtg ctgcgcgtgt tcctgatcca gggcggcgcg atggggctcg cgggctcggt
     5341 gctcggctcg ctgctcgcct ggggcttcct gctggtctgg cgcgccgttg cgaggaatcc
     5401 ggacgggacg ccgatgttcg tcatccagat ggagccgtcc ctgttcgcga tcgcgactgc
     5461 gggtgcgacg ctcgtgggga tactggcagc ggtggcgccg gcgcgccgcg cc
//
LOCUS       DBNGSG010000475         4926 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3379582, whole genome shotgun sequence.
ACCESSION   DBNGSG010000475 DBNGSG010000000
VERSION     DBNGSG010000475.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4926)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4926
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3379582"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /gene="thiD"
                     /locus_tag="AC158Q_12600"
     CDS             complement(<1..691)
                     /gene="thiD"
                     /locus_tag="AC158Q_12600"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:NF020132.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0042724 - thiamine-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HIR3340658.1"
                     /translation="MADLPPIVLVFAATDPSGGAGLQADIMTLSSMGCHPLSVVTAVT
                     IQDTMGVDDVSPIEAEWVIDQARCVLEDMPVAAFKIGLLGNIEQIAAIAEVVSDYPEV
                     PLVLDPVLASGRGDELVTEEMVVALKELLIPQTTILTPNSLEARRLAAEEGEEGEGLD
                     FAECAKRIIAGGCEYVLVTGAHENTPEVVNTLYGQNGVVRSDSWRRLPGTYHGSGCTL
                     ASAIAATIANGL"
     gene            777..965
                     /locus_tag="AC158Q_12605"
     CDS             777..965
                     /locus_tag="AC158Q_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0043448 - alkane catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HIR3340659.1"
                     /translation="MTATETQVYKTYMCLICGFLYDEAAGLPEEGIAPGTRWEDVPPN
                     WTCPECGARKDDFEMVEV"
     gene            973..1365
                     /gene="gloA"
                     /locus_tag="AC158Q_12610"
     CDS             973..1365
                     /gene="gloA"
                     /locus_tag="AC158Q_12610"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HIR3340660.1"
                     /translation="MRILHTMIRVGDLQRSIDFYTQVLGMRLLRKADYPSGRFTNAFV
                     GYDEESKTAVLELTHNWDTKSYDLGSGYGHVAIEVADAYKACEEVKQRGGRVTREAGP
                     MKHGTTVIAFVEDPDGYKIEFVQRKAAV"
     gene            complement(1362..2138)
                     /gene="rsmA"
                     /locus_tag="AC158Q_12615"
     CDS             complement(1362..2138)
                     /gene="rsmA"
                     /locus_tag="AC158Q_12615"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004522793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HIR3340661.1"
                     /translation="MRHVPRKRFGQNFLTDESAIAAIVAAVSPQRDDLMVEIGPGLGA
                     LTRPLAARLDHLHVIEIDRDIIARLRIAFPAARVTVHEGDALNFDFGALGAPLRVIGN
                     LPYNISTPLLFHLARYASTIRDIHVMLQKEVVERMVSAPSCAEYGRLSVMLQYRFEME
                     RVLDVPAHAFHPVPKVESAVVRMTPFARLPHPARDEGALGKIVAAAFSQRRKTLRNAL
                     SGYFKLGDFERLGVDARLRAQDLRVEDFVRLADDRSAAAG"
     gene            complement(2159..3148)
                     /gene="pdxA"
                     /locus_tag="AC158Q_12620"
     CDS             complement(2159..3148)
                     /gene="pdxA"
                     /locus_tag="AC158Q_12620"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011657883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HIR3340662.1"
                     /translation="MAVTAGEPAGIGPDLCVALARRRLPARIVVVADRELLKDRARRL
                     RVPLRLADFPSANAVKPVAGALVVSHVPLERPVFAGRLDPSNSRYVLRTLEVAADGCR
                     DGIFDAMVTAPLQKSVINDAGIPFTGHTEYLAERTGTRHVVMMLVGGGMRVALATTHL
                     ALKDVARSITREGLERTLRVLQQDLIGRFSINKPRTLVAGLNPHAGESGFLGREETEI
                     IVPVLEKLRRAGFDLAGPFPADTLFQPKRLKGCDCVLAMYHDQGLPVLKYASFGAGVN
                     VTLGLPIIRTSVDHGTALDLAGTGRAEVGSLVEAIKLAAALAGGGRNRNRRAA"
     gene            complement(3240..4577)
                     /locus_tag="AC158Q_12625"
     CDS             complement(3240..4577)
                     /locus_tag="AC158Q_12625"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR3340663.1"
                     /translation="MVEVLSQHPYRGSLLQVLLLAAALAPITTAALAQQPRKVIVLDR
                     IVAVVNNEVITRGDLEERTRFVTLQLKQQGTPLPPREVLERQTLERMISGRVQLQFAK
                     ETGLRVSDGELDRAIQRIAQENKLSVEGLRGALEKDGMPFSRFREDIRDEIVLARLRE
                     REVDNRVVVTESEIDNFISTQQSQTDRGEEFNISHILVTVPENASPEQIRARRDRAEQ
                     ALEQIKSGTDFRQVAASFSEAPDALQGGVMGWRDAARMPSLFLDAVKTMQAGTVSPLL
                     RSANGFHILRVNERRGSAAPVIVRQTRARHILIRTNELVPEAEARRRLVSLKERLDNK
                     ADFAQLARQYSEDASNARGGDLGWLSPGDTVPEFERALNALKPGETSDPVQTPFGWHL
                     IQVLEHRNEDMSRERLRLDARKALHARKSDESYQEWVRQQRDLAYVDYRLEER"
     gene            complement(4595..>4926)
                     /locus_tag="AC158Q_12630"
     CDS             complement(4595..>4926)
                     /locus_tag="AC158Q_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340664.1"
                     /translation="TDISAQWPVGQGWTALARWNYSLIDQRTLEGLAGAEYNGDCWAL
                     RIVAHRFATATQQASTTFFIQLELNGVSRIGSNPMDTLKRNIGGYARLDPRAPRPRPD
                     EPRTPYY"
BASE COUNT          875 a         1573 c         1620 g          858 t
ORIGIN      
        1 cgagcccatt ggcgatggtg gcggcgatgg cggacgcgag cgtgcagccc gagccatggt
       61 aggtgccggg caggcgccgc caggagtcgc tgcgcaccac gccgttctgg ccgtaaaggg
      121 tgttgacgac ctcgggagtg ttttcatgag cgccggtgac gaggacgtat tcgcaaccgc
      181 ccgcaatgat ccgcttggcg cactcggcaa aatcgagccc ctcgccttcc tcaccctctt
      241 cggctgcgag ccgtcgcgcc tccaggctgt tgggggtcag tatggtggtc tggggtatga
      301 ggagctcctt cagcgcgacc accatctcct cggtgaccag ctcgtcaccg cggcccgagg
      361 caagtaccgg atcgaggacc agcggcactt cagggtaatc ggaaacgact tccgcgatcg
      421 cggcgatctg ctcgatgttg cccagcagtc cgatcttgaa ggcggctacc ggcatgtctt
      481 cgagcacgca gcgcgcctga tcgatgaccc actccgcttc gatcggagaa acgtcatcca
      541 cgcccatggt gtcctggatg gtgaccgcgg tcaccaccga gagcggatgg caacccatgc
      601 tcgagagcgt catgatgtcc gcctgcagcc ccgcgccgcc gctcggatcg gttgcggcga
      661 ataccagaac aatgggaggc aggtcggcca tgcgtcggga gaattcggat gcccgcggtt
      721 ggtcgcggga gggcgattcg atagaattgg attctaactg aattacggac aattgaatga
      781 ccgccaccga gacccaggtt tacaagactt acatgtgcct catctgcgga tttctctacg
      841 atgaggccgc cgggctgccc gaggagggca tcgcacccgg cacgcgctgg gaggacgtac
      901 cgccgaactg gacctgcccg gaatgcggcg cgcgcaagga cgatttcgaa atggtggagg
      961 tttgaggcga ccatgagaat cctgcatacc atgatccgtg tcggcgacct gcagcgctcg
     1021 atcgacttct atacccaggt gctggggatg cggttgctgc gcaaggccga ttatcccagc
     1081 gggcgcttta ccaacgcttt cgtcggctac gacgaggagt cgaagacggc ggtgctcgag
     1141 cttacccaca actgggatac gaaatcatac gatctgggca gcggctacgg ccacgtggcg
     1201 atcgaagttg cggacgccta caaggcatgc gaagaggtga aacagcgcgg gggcagggtg
     1261 acgcgggaag ctggacccat gaagcatggc accaccgtga tcgcctttgt cgaggacccc
     1321 gacggctaca agatcgagtt cgtccagcgc aaggcggcag tttagccggc ggcagcggac
     1381 cggtcgtccg ccaggcggac gaaatcctcg acccgcaagt cctgtgcccg cagccttgcg
     1441 tccacgccta gccgttcgaa atcgcccagc ttgaaataac cgctcaacgc gttgcgcagc
     1501 gtcttgcggc gctgggagaa ggcggccgca acgatttttc cgagcgcgcc ttcatcgcgc
     1561 gcggggtgcg gcagccgcgc gaacggcgtc atgcgcacca ccgccgattc gaccttgggc
     1621 acgggatgga atgcgtgcgc agggacatcg agaacgcgct ccatctcgaa acgatactga
     1681 agcatgaccg acaagcgtcc gtattcggcg caagacggcg cggagaccat gcgttcaacc
     1741 acctccttct gcagcatgac gtggatgtcc cggatagtgc tggcgtaccg tgccaggtga
     1801 aacagcagcg gcgtggaaat gttgtaaggg aggtttccga tcacgcgcaa cggggctccc
     1861 agcgcgccga aatcgaaatt gagggcatcg ccctcgtgca ccgtcacgcg cgcagccgga
     1921 aatgcaattc gcaaacgggc gatgatgtcg cgatcgatct cgatgacgtg caggtggtcg
     1981 agtcgcgcgg cgagcggccg cgtgagcgcc ccgagacccg gtccgatttc caccatgaga
     2041 tcgtcgcgct gcggcgacac ggcggcgacg atggccgcga tcgccgattc gtcggtcagg
     2101 aaattctgcc cgaatcgctt gcgcggaacg tggcgcatga atcttcgctg cgcgcgcttt
     2161 acgcggcgcg gcgattccgg ttgcggccgc cgccggcgag tgcggcggca agcttgatgg
     2221 cttcgacgag gctgccgact tcggcccgtc cggtgccggc gagatcaagc gcggtgccgt
     2281 ggtccaccga ggtgcggatg atgggaaggc ccagcgtcac gttgaccccg gcgccgaaac
     2341 tcgcgtactt gagcaccggc aggccctgat cgtgatacat cgcgagcacg cagtcgcagc
     2401 ccttgagccg cttcggctgg aacagggtgt cggcgggaaa ggggcccgcg agatcgaagc
     2461 ccgcgcggcg cagcttttcg agcaccggca cgatgatctc agtttcttcg cgcccgagaa
     2521 agcccgactc cccggcatga gggttgaggc cggccactaa ggtacgcggc ttgtttatgg
     2581 aaaatcgccc gatcagatcc tgctgcaaca cccgcagcgt gcgctcgagt ccctcgcgcg
     2641 taatgctgcg ggcgacatct ttcagcgcga ggtgggtggt agcgagcgcg acccgcatac
     2701 cgccgcctac cagcatcatc accacgtgcc gcgtgcccgt gcgctcggcg agatactcgg
     2761 tgtggccggt gaacgggatg cctgcgtcgt tgatcacact cttctgcagc ggcgctgtca
     2821 ccatcgcgtc gaaaatacca tcgcggcagc cgtcggcggc gacttccagg gtgcgcagca
     2881 cgtaacggct gttggaaggg tcgagacgcc cggcgaaaac gggccgctcg aggggtacgt
     2941 ggctcaccac cagcgcaccc gcaacgggtt tcacggcatt ggcagagggg aaatccgcca
     3001 gcctcagcgg gacgcgcagg cgccgggcac ggtctttcag cagctcccgg tcagccacca
     3061 ccacgatgcg ggccggcagc cgccgccgcg caagcgcgac gcacaggtcg ggcccgatgc
     3121 ccgcaggctc gcccgccgtc acggcgatca gcggaacagg gagactggat cgtttggttt
     3181 tcgcttgcac ggaggccggc cacagcgcgc agccggtaac ttaccattaa aaacgcccgc
     3241 taccgctctt cgaggcggta atcgacgtaa gccagatcac gctgctgccg cacccattcc
     3301 tggtaggact cgtcggactt gcgcgcgtgc agcgcttttc tcgcgtcgag ccgcaggcgc
     3361 tcgcgcgaca tatcttcgtt tcgatgctcc agcacctgga tcagatgcca cccgaacggc
     3421 gtctggaccg ggtcgctggt ttcacccggc ttgagcgcgt tcagcgcgcg ctcgaactcc
     3481 ggcaccgtgt cgcccggaga cagccatccc aggtcaccgc ccctggcgtt actggcatcc
     3541 tcggaatact ggcgcgccag ctgcgcgaaa tcggccttgt tgtccagacg ctccttgagg
     3601 ctcaccaggc gtcggcgcgc ctcggcctcc gggaccagtt cgttggtcct gatcaggata
     3661 tggcgcgcgc gggtctggcg gacgatcacc ggggcggcgc tgccgcggcg ctcgttgact
     3721 ctcaggatgt ggaagccatt ggcgctgcgc aacaggggac tgaccgtgcc ggcctgcatc
     3781 gtcttgaccg cgtccagaaa cagcgatggc atgcgcgcgg catcgcgcca gcccataacc
     3841 ccgccctgca acgcgtccgg cgcctcggaa aaactggcgg ccacttggcg gaagtcggtc
     3901 ccggacttga tctgctcgag cgcctgctcg gcgcggtcgc gccgcgcccg gatctgctcg
     3961 gggctggcgt tttccgggac ggtcaccaga atgtgcgaga tgttaaattc ctcgccacga
     4021 tccgtttgcg actgttgggt gctgatgaaa ttgtcgatct cgctttcggt cacgaccacc
     4081 ctgttgtcca cctcgcgttc gcgcagccgt gccaggacga tttcgtcgcg aatgtcctcg
     4141 cggaatcggg aaaagggcat gccgtccttt tccagcgccc cccgtaatcc ctcgaccgaa
     4201 agcttgttct cctgggcaat gcgctggata gcgcggtcga gctcgccgtc gctcacccgc
     4261 agtcccgttt ccttggcgaa ctggagctgc acgcggccgg agatcatgcg ttcgagggtc
     4321 tgccgctcca gcacttcgcg cggcggcagc ggcgtaccct gctgcttcag ttgcagcgtg
     4381 acgaaccggg tgcgctcttc caggtcgccg cgcgtgatca cttcgttgtt taccaccgcc
     4441 acgatacgat cgagcacgat gaccttgcgc ggttgctgcg cgagagcggc cgtcgtaatc
     4501 ggcgccagcg cggcggcgag cagcagaact tgcagcaggg agccgcggta cggatgctgg
     4561 gataggactt caaccataaa agtgccttgc gggatcagta atacggcgtc cgcggctcgt
     4621 cggggcgggg ccggggcgcg cggggatcca gtctggcata accgccgatg ttgcgcttga
     4681 gcgtatccat gggattagag ccgatgcgcg acacgccgtt caattcgagc tggatgaaaa
     4741 acgtcgtgga ggcctgctgc gtggccgtgg caaaacggtg ggcgacgatg cgcagggccc
     4801 agcagtcgcc attgtattcc gcgcccgcca gcccctcgag cgtgcgttga tcgatcagcg
     4861 agtaattcca ccgcgcaagc gccgtccacc cctggccgac cggccattgc gcggaaatat
     4921 cggtct
//
LOCUS       DBNGSG010000476         3227 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2191692, whole genome shotgun sequence.
ACCESSION   DBNGSG010000476 DBNGSG010000000
VERSION     DBNGSG010000476.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2191692"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..1439
                     /locus_tag="AC158Q_12635"
     CDS             270..1439
                     /locus_tag="AC158Q_12635"
                     /inference="COORDINATES: protein motif:HMM:NF005788.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF family hydroxylase"
                     /protein_id="HIR3340665.1"
                     /translation="MRHSFDVVIVGGGLVGLSLARALADSGLALALVESQSPGAIPGE
                     ESWDNRIYAVSPGCASFLERCGAWGRLPQGRLTRIEAMQVYGDVAGACLEFSAYDAGL
                     RELAFVVESRRLQHALWEVARGQDIQVYCPASWTGLEFHDDRAVLRLDDGTELAARLV
                     AGADGSESRVRAAAGIASVPAEYGQLGVVANFSCERPHRGTAFQWFMRDGVLALLPLP
                     DNRVSMVWSVEQERGRSLLALPAAELATEVESASRAALGALRLMAPAAAFPLRLARVA
                     QFTKPRVALVGDAAHNVHPLAGQGVNLGFRDARELARVLVERGPQRDCGDHALLRRYE
                     RARREDVAAMQLTTDGLQKLFNNDAVLLTKARNLGLEFVNRQPWLKNFLVHHAVA"
     gene            1461..2180
                     /locus_tag="AC158Q_12640"
     CDS             1461..2180
                     /locus_tag="AC158Q_12640"
                     /inference="COORDINATES: protein motif:HMM:NF024498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003756 - protein disulfide isomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HIR3340666.1"
                     /translation="MLRKFAFVLPMLLAGLAAQAALGSEASIRQAFQSRIPEVTVESV
                     GRTPMPGLYEIVANGHIFYVDEKVNFVIRGTLFDARTPAMRNVTMARNSELTAQALSK
                     SNANAIKRVRGNGKRVIYTFEDPNCGYCKKQQQELIKLNDVTIYTFLWPILSPDSIEK
                     SKAIWCSRDRAKAWEDLMLRGVSPQNETRCDTPIEKNMEMARRFGAQGTPAVYLADGR
                     MIGGFVAADKIEEIFRFPAEK"
     gene            2560..2916
                     /locus_tag="AC158Q_12645"
     CDS             2560..2916
                     /locus_tag="AC158Q_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340667.1"
                     /translation="MERGAAPPPALPQVPDPTVYTARDLDSYPRPVAPLDFGRPAERA
                     AGLPIAEIRLELLIDERGIVGDIAFAEPRLSSQLETQLRTAVASTRFVPARRNGRAVK
                     SRVLLSVNLGQEKRDP"
     gene            complement(2955..>3227)
                     /locus_tag="AC158Q_12650"
     CDS             complement(2955..>3227)
                     /locus_tag="AC158Q_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HIR3340668.1"
                     /translation="LTARRSVAVDPGFVPLGAPVYIATTWPLSTRPLNRLMLAQDTGG
                     AIRGPVRADFFWGFGSEAAREAGRMKQPLRMWVLLPNAFRVPQSEP"
BASE COUNT          551 a         1023 c         1076 g          577 t
ORIGIN      
        1 gtgcaggcgt gtgtgagtcc cgcgcaattg ctggcgctca cctgtcccgc tccggggtca
       61 catccaggca caaaccgcct ggaacctgct ccgccctgcg cgggacactt tgtcggccgg
      121 cttggccata ttcgcgatag gccctgcgcc gtccttcgcg tcatcgccca ttttggctcg
      181 caacgcacgc atgtttcatt gtgttaggga ctctaaggta tcccgcgcta aactgcgcgg
      241 accatgatga gttagggcga tttcaacgca tgcgtcactc tttcgatgtc gtcatcgtcg
      301 gcggcgggct ggtcggcctg agtctcgcgc gcgcgctcgc ggacagcggg ctcgctctcg
      361 cgctcgtcga atcccagtca ccgggcgcga ttcccgggga agaatcctgg gataaccgca
      421 tctatgcggt gagcccgggc tgcgcctcgt ttctcgagcg ctgcggcgca tgggggcgcc
      481 tgccgcaggg gcgtttgaca cggattgagg cgatgcaggt gtatggcgat gttgcgggcg
      541 cctgtctcga gttcagcgcc tatgacgccg gcttgcgcga gctcgccttc gtcgtcgaaa
      601 gccgccgcct gcagcatgcg ttatgggaag tcgcacgcgg ccaggatatc caggtttatt
      661 gtcccgccag ctggaccggc ctggaatttc atgatgaccg tgccgtgctg cgcctcgacg
      721 acggtaccga gcttgcggcg cgcctggtgg caggtgccga tggctcggag tcccgggtgc
      781 gcgcggcagc cggcattgcg tcggtgcctg ccgagtacgg gcaactcggc gtggtcgcca
      841 atttttcctg cgagcgaccc caccgcggca ccgctttcca atggttcatg cgcgacggcg
      901 tgctcgcgct gctgccgctg ccggacaatc gcgtctcgat ggtgtggtcg gttgagcagg
      961 aacgcgggcg cagtctgctc gcgctgccgg ctgcggaact ggccacggaa gtcgaatcgg
     1021 cgagccgcgc cgcgctcggc gcgttgcggc tgatggcgcc tgccgccgca ttcccgctca
     1081 ggctcgcgcg cgtcgcgcaa ttcacgaagc cgcgggtggc gcttgtcggg gatgccgctc
     1141 ataatgtgca ccccctggcg ggacagggag tcaatctcgg tttccgggac gcgcgtgagc
     1201 tcgccagggt gctggttgaa cgcgggccgc agcgagactg cggcgaccac gcgctgctga
     1261 ggcgctacga gcgcgcgcgc cgggaagacg ttgccgccat gcagctgacg accgacggcc
     1321 tgcagaaact tttcaacaac gacgctgtct tgctcacgaa ggcgcgcaac ctgggactcg
     1381 agttcgtcaa ccggcagccg tggctcaaga acttcctggt gcatcacgcc gtcgcctgac
     1441 gaatacgacc aaggataacc atgctacgaa agttcgcgtt tgtcttaccg atgctgctgg
     1501 cggggcttgc agcacaagcc gccctgggca gcgaggcgtc catccgccag gcgtttcagt
     1561 ccaggattcc cgaggtgacg gtggagagcg tgggcaggac gcccatgccc ggcctctacg
     1621 agattgtcgc gaacgggcat atcttctacg tcgatgagaa ggtcaacttc gtgatccgcg
     1681 gcacgctgtt cgatgccagg acgccggcga tgcgcaatgt cacgatggcg cgcaactcgg
     1741 agttgaccgc gcaggccctc agcaaatcga atgccaacgc gatcaagcgc gtgcgcggca
     1801 acggcaagcg cgtgatctat acattcgaag atcccaattg cggctactgc aagaaacagc
     1861 agcaggaact gatcaagctg aacgacgtca ccatatacac gttcctgtgg ccgatcctct
     1921 cgccggattc gatagagaaa tcgaaggcga tctggtgctc ccgggatcgc gccaaggcat
     1981 gggaagatct catgttgcgc ggcgtttccc cgcagaatga gacccggtgc gacaccccga
     2041 tcgaaaagaa catggagatg gcccggcgtt tcggcgcgca aggcacgccg gccgtctatc
     2101 ttgcggacgg gcgcatgatc ggcggctttg tggcggcgga taaaatcgag gagatcttca
     2161 ggtttccggc cgagaagtga cccgttcggg gcggttcggc ccggaaacag tcaacagggg
     2221 atgacgccag gcgttattcc ctgaatgacc agccgagtag ctccaccttc ccggcatttg
     2281 ctctggtccg ccgaaggccg catagggccc gcgctggcgg cctcggtgat tatccacctg
     2341 ttgctggcgg cagccccggt aacagggatg tcaccgcgag ccgcgcaatc tcaaggtgtt
     2401 gcgttgatca cggcatggat cgaacctctg ccagggtcgg ggccggacca cgcggctgcg
     2461 gcgcatagag cggaaattcc cgggccgcag ccggccaagc gcgattttgc gatttccgac
     2521 gatacgcggc gcgagacggc tccgaatgct gcctcgggga tggagcgggg cgccgcgcca
     2581 ccgcccgcgt tgccgcaggt gcccgatccg acggtataca cggcgcgcga tctcgacagc
     2641 tacccgagac cggttgcccc gctggatttt ggccggcctg ccgagcgcgc cgcgggattg
     2701 ccgattgcgg aaatcaggct cgagctgctc atcgatgagc ggggcatcgt gggcgacatt
     2761 gcattcgccg aaccgcggtt atcgagtcag ttggagacac aactgcgcac tgccgtcgcg
     2821 tccacgcgtt tcgtccctgc gcgcaggaac gggcgtgccg tgaaaagccg cgtgttgctg
     2881 agcgtgaatc ttggccagga aaagcgtgat ccgtgatcag cagctccccg cccgcgggtc
     2941 taaaaacagt cggttcacgg ttcgctctgc ggtacgcgaa aagcgttggg cagcagcacc
     3001 cacatcctga gcggctgctt catgcgcccg gcttcgcgcg cggcttcact cccgaatccc
     3061 cagaaaaaat ccgcccgcac cgggccgcga atcgcgccgc cggtatcctg ggccagcatc
     3121 aggcggttga gcggccgggt ggaaagcggc caggtcgtgg cgatgtacac cggggcgccc
     3181 agcggaacga agcccgggtc caccgccaca ctgcggcgcg cggtgag
//
LOCUS       DBNGSG010000477         5394 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4059290, whole genome shotgun sequence.
ACCESSION   DBNGSG010000477 DBNGSG010000000
VERSION     DBNGSG010000477.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5394)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5394)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5394
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4059290"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="AC158Q_12655"
     CDS             complement(<1..209)
                     /locus_tag="AC158Q_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3340669.1"
                     /translation="MLFKPVILWTDALVFLLVAAVVALTWHVRRHEHLAAPWRKVARD
                     PVGMAAATVLALFVAIGLADSLHYRP"
     gene            complement(256..1233)
                     /locus_tag="AC158Q_12660"
     CDS             complement(256..1233)
                     /locus_tag="AC158Q_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3340670.1"
                     /translation="MISYIIRRLLYAIPILIGVNVITFTLFFVVNSPDDMARVHLGVK
                     RVTPEAVKKWKEERGYDRPLLYNGAAAGAKQLTETIFFEKSVKLFAFEFGRSDQGRDI
                     GYDIGNRMWASLAIALPVFLLGLIAYITFALLMAFFRATSVDFWGVVACVTMMSISGL
                     FYIIGGQYLVGKLLNIVPISGYAGGADAFKVLALPVIVGIVSGIGANTRWYRTIFLEE
                     VNKDYVRTARAKGLSELRVLFRHVLANAMIPILTGVVVVIPLLFMGSLVAEAFFGIPG
                     LGSYTIDAINSQDFAVVRAMVFLGSVLYIIGLILTDISYTLVDPRVRFE"
     gene            complement(1446..3623)
                     /locus_tag="AC158Q_12665"
                     /pseudo
     CDS             complement(1446..3623)
                     /locus_tag="AC158Q_12665"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            3834..4625
                     /gene="fabI"
                     /locus_tag="AC158Q_12670"
     CDS             3834..4625
                     /gene="fabI"
                     /locus_tag="AC158Q_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020423770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HIR3340671.1"
                     /translation="MGLLQNRKILVTGLLSNRSIAYGIARALHREGATLAFTYQAADL
                     RDRVEGLAREFGTAPVLPCDVASDGEIAALFAELGARWDGLDGIVHSVAFAPRDALKG
                     DFLDGLTREAYRLAHDVSAYSFPALAKAALPLMEGRRGALLTLTYLGAERAVPHYNVM
                     GMAKASLEAGVRYLAEGLGPKGIRVNGISAGPIKTLAAAGISGFGKILKFVEDNAPLR
                     RNVTIDEVGNTAAFLMSDLASGITGEITYVDAGFNTVVGGMRGEA"
     gene            complement(4669..>5394)
                     /locus_tag="AC158Q_12675"
     CDS             complement(4669..>5394)
                     /locus_tag="AC158Q_12675"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl isomerase family protein"
                     /protein_id="HIR3340672.1"
                     /translation="QLAARRFAELAEGFSNMAYEQSDSLKPAAELVKAQPRSSGWISR
                     SSAEAPLNNPRLLNAIFSDEVLKDRRNTEAVEVGPSVLVAARVIEHKPSSIQPFEEVR
                     AGLEKKLALREATRLAVEDGRRQLEALRQGKPATVAWSTPLVVSREEAKDLPAPVLRQ
                     AFRMDASKLPAYAGVDGPRAAYVLLRVTRVQEAGDTPPEKLKSISGQLRQVLGEEALS
                     AYLASLKQKAGVTINKEQLEKKQ"
BASE COUNT          970 a         1712 c         1742 g          970 t
ORIGIN      
        1 ggccggtagt gcagcgagtc ggcgagcccg atggcgacga acagggcaag cacggttgcc
       61 gccgccatgc cgaccgggtc gcgcgccacc ttgcgccaag gcgccgccag gtgctcgtgc
      121 cgccgcacgt gccaggtgag cgccacgacc gccgcgacca gcaggaacac cagcgcgtcg
      181 gtccacagga tgacgggttt aaacagcata ggtcaaaaaa tagacaggat tcacaggatt
      241 catcccgtta catttttact caaagcgcac cctcggatcg accagcgtgt acgaaatgtc
      301 ggtgagtatc agaccgatga tgtagagcac ggaccccagg aacaccatcg cgcgcaccac
      361 ggcgaagtcc tgcgagttga tggcgtcgat ggtgtagctc ccgaggccgg gaatgccgaa
      421 gaaagcctcg gccaccaggc tgcccatgaa caatagcggg attaccacca ccacgccggt
      481 gaggatcggg atcatcgcgt tggcgagcac gtgacggaac agcactcgca gctccgagag
      541 gcccttggcg cgcgcggtgc gcacgtagtc cttgttgacc tcctccagga agatggtgcg
      601 gtaccagcgc gtattcgcgc cgataccgct tacgatgccg acgatgacgg gaagcgccag
      661 gaccttgaac gcgtcggcgc cgcccgcata gcccgaaatc ggcacgatgt tgaggagctt
      721 gccgacgagg tactgcccgc cgatgatgta gaacaggccc gagatggaca tcatcgtcac
      781 gcaggcgacc acgccccaga aatccacgga ggtcgcgcgg aagaacgcca tcagcagcgc
      841 gaacgtaatg taggcgatga gtcccagcag gaaaaccggc agcgcaatgg ctaggcttgc
      901 ccacatgcgg ttgccgatgt catagccgat gtcgcgcccc tggtcggagc gaccgaactc
      961 gaaagcgaac agcttcaccg acttctcgaa gaaaatcgtt tcggtgagct gcttcgctcc
     1021 cgctgccgcg ccgttgtaca ggagcggccg gtcgtagccg cgctcctcct tccatttttt
     1081 cactgcttcc ggcgtcacgc gcttcacccc caggtgcacc cgcgccatgt cgtcgggcga
     1141 gttgacgacg aaaaacagcg tgaaggtgat aacgttgact ccgatcagga tcggaatcgc
     1201 gtagagcagg cggcggatga tgtaggaaat cacttctact acccaaaggc gtgattcgtg
     1261 aatagtgaat ggtgaatggt gaatgcaccc aagctcacat caggccgctc catccgggtt
     1321 aagggtgcgt aacgaattga ggggagtaac ccctcatgct cccccacatc aggggccgta
     1381 tcattcattt cgacaccggc cccaagaaaa attggggttt ccattcacta ttcaccagtc
     1441 accattcacg ctttcgcggt ctggcgttcg cggcgccgga aggttcgcaa cgccggcacg
     1501 aacgacagcg tcaccgcggc caagaacgcc agggccggcc acaggaccgg ccggttccat
     1561 tcgcgccgca tctgctcccg cgccaccgga tcgatgcgct ggtacttgag cccgttgttc
     1621 gcgatcgaat ggggcttgcg gttgcggacc caggcgtggt ggagcgtgta gtccttcgga
     1681 aagaagctcc acacccacgg cgcgtcgtgg cgcgcgactt cgaccatgcg gtcgatgatc
     1741 gccgcacgct cgggaccgtt gcccatgctc ttcattcgct cgaacaggcg gtcgaactcg
     1801 gggttctccc agttggaggc gttctcgccg gaggtcttga ccttgctctg cgcgccgtgg
     1861 agcaggaaca ggaagttctc ggggtcggga taatccgcgt tccagccgag gaaatagagc
     1921 tgcacgttgc ccttgcggat cgtgtcctgg aagcggttcc agtcggtggc gcggatcgcg
     1981 agctgcacgc cgatcttctc gaactgcttc gcataccagt cgaggcgcga cttggcgtcg
     2041 ggtccggtgc cggtagtgtc gaggttcacc agcagcggct agccggtttt cgcgtcgcgc
     2101 ccgccaggat agcccgcctc ggccagcagg cgcttcgcgt gctcgacgga cttcctgcgc
     2161 ggggcgccgt ccgcccagtc gtagacgtag ggattgatcc ccgcttttcc ctcctggtag
     2221 ccgaagatgc cgggcggaat cgggccctgg gcagcaatgc cgcgcccgtt gcggaagatc
     2281 gagatgaatt cctcctgatc caccgcgatc gagatcgcca gccgcagctt gcgcgctcgc
     2341 gcgcgcgcct gctcgcttcc ccccagcccc cccacccccg ggtcgcgcat ggtgaatccg
     2401 aggtagaaaa cggaagtggc gaccgaggtc tgcagccgga tatcgcgcgc cagcatctcc
     2461 tccgacagcg tggcatcgcc ctggcctgag aactgcaccg cctggtcgaa cgtatcggaa
     2521 ctgatgctgg atgcgtcgta atagccctgc aggaacttgt tccagtacgg gatctgctcc
     2581 ttctcgaggg agaacaccac cttgtccacg aacggcagcg gcttgcccgc gtccgcgagg
     2641 aagccggctt ccgcgtctcc cggttcgccc tcggaggggt aaacctctcc gcgatagttg
     2701 ggattcttct cgaggaccat ctgccggttg ggattgttga ccgacagcat gtaggggccg
     2761 gtgccgaccg ggaaccagtc gagcgagatg ttcttctccg ccatccccgg ttgcgcgtag
     2821 aagcgatcgg actcgggcgg gacaggcgcg aagaacggca tcgccagcca gtagacgaac
     2881 tggggatact tgcccttgac gcggatgcgg taggtgtagc ggtccacgac ctgggcgccc
     2941 gcaagctcga agcgctcgag gtcgaggaac ccgcccgcgg gcagcgcctt atccgccttg
     3001 gcgagggtcg ccgcaagctc cttcagcccg acgatgtatt ctcccatgag ctgcatgatc
     3061 ggcgagtgca ggcgcggatg agccaggcgc ttcaactggt gcacgtagtc ggcggcgacg
     3121 agctcgcgcg ttcccgcgag cctgaaatcg ccgatggcgt gaatcccgtc gagatcgccc
     3181 ggcttgagat cgtggtaggc gtggcgctcc tgcgcgtcct tcgccagcgc cgggtgcggc
     3241 tggtagagga ttccgggccg gatacggacc acatagtcgc tgtacgcgac ctgcttcgcg
     3301 tcggcgcttt cgggcaggcg cctgcccttc gcgtcgtgga actgcggacg tggcacctcc
     3361 gtggcggaga acgggacgag ctcgtaaggc cgcttgaagt agtgatactg cagcggcggc
     3421 tggtagatgt tggcaatcaa cacgtactcg ttctcgctgt acgattgcac cggatcgagg
     3481 tgcttgggcc gctcggagaa cgaggagtag agaatgtttg cgccggcttc cgacgccggg
     3541 tggggattgt tccacggcgc ctcgctgcaa ccgaccagca gcgcgagcat cgcgagcgct
     3601 gcgcgtagga tggggggcgg catcgctgaa tattaccaaa ggcgtgattc gtgagtcgtg
     3661 attcgtgatt cgaattgccc ctcacccgct tccctgtcgg ggtagcgtaa agtcaagatt
     3721 cgattcacga ctcacgattc acgggaatag agggtcgaat cacgactcac gccttataat
     3781 tggaagcgcc gcccccgtgt tcgtcccggg gtcactcaga acaaggaaga agcatgggct
     3841 tgctccagaa caggaaaatc ctcgttaccg ggctgctgtc caaccgctcc attgcctacg
     3901 gcatcgccag ggccttgcat cgcgagggcg caaccctggc gttcacctac caggccgcgg
     3961 acctgcggga tcgcgtcgag gggctggccc gggaattcgg cacggcgccg gtgctgccct
     4021 gcgacgttgc gagcgacggg gaaatcgcgg ccttgtttgc cgaactgggc gcgcgctggg
     4081 acgggctgga cggcatagtg cattccgtcg cctttgcgcc gcgagatgcc ctgaagggcg
     4141 attttctgga cggcctcaca cgcgaggcgt atcgcctcgc gcacgacgtc agcgcctaca
     4201 gcttccccgc cctggcgaaa gccgcactgc cgttgatgga ggggcgccgg ggcgcgctgc
     4261 tcaccctcac ctacctcggc gcggagcgcg cggtgccgca ctacaacgta atgggcatgg
     4321 ccaaggcgag cctggaagcc ggcgtgcgct acctggccga gggcctcggg ccgaagggca
     4381 tacgcgtgaa cggcatttcg gccgggccga tcaagacgct cgcggccgcc ggcatcagcg
     4441 gctttggcaa gatcctgaag ttcgtcgagg acaatgcgcc gctgcggcgc aatgtgacga
     4501 tagacgaggt cggcaacacc gcggcgttcc tgatgagcga cctcgcatcc ggcatcaccg
     4561 gtgagatcac ctacgtcgac gcgggcttca atacggtcgt cgggggaatg cggggcgaag
     4621 cctgagcggc ggttgatggg gttgaatcgg gattctgcca gtcacccgtc actgcttctt
     4681 ctcgagttgt tccttgttga tggtcacgcc ggccttctgc ttgaggctcg ccaggtaagc
     4741 tgacaacgcc tcctccccca gcacctggcg caactgaccc gagatcgact tcagcttctc
     4801 cggcggggta tcgccggctt cctggacccg ggtaacgcgc agcagcacat aagccgccct
     4861 cgggccgtcc acacccgcgt aagcgggcaa tttcgaggcg tccattcgga atgcctgacg
     4921 cagaaccggc gcgggaaggt ccttggcttc ctcgcggctt acgaccaggg gggtgctcca
     4981 cgcgaccgtg gcgggcttgc cctgcctgag cgcctcgagc tggcgccggc cgtcctccac
     5041 cgccaatcgt gtcgcctcgc gcagcgccag tttcttctca aggcctgcgc gcacttcctc
     5101 gaacggctgg atgctggacg gcttgtgctc gatgacacgc gccgcaacca ggacgctggg
     5161 cccgacttcg accgcttcgg tattgcgccg gtccttgagc acctcgtcgg aaaagatcgc
     5221 attgagcagt ctgggattgt tgagcggcgc ctcggcgctg gagcggctga tccagccgct
     5281 gctccgcggc tgggccttta ccagctccgc agcgggcttg aggctgtccg attgctcgta
     5341 agccatgttg ctgaagcctt ccgccagctc cgcgaagcgc cgcgccgcca gttg
//
LOCUS       DBNGSG010000478         4513 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3381003, whole genome shotgun sequence.
ACCESSION   DBNGSG010000478 DBNGSG010000000
VERSION     DBNGSG010000478.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4513)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4513)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4513
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3381003"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="AC158Q_12680"
     CDS             <1..467
                     /locus_tag="AC158Q_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011851735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIR3340673.1"
                     /translation="GGGFDLPVMHYRGLIHGVKAGRYWDLGEDDRDFKWNNYISRYHS
                     RHLDLMDLLALYQPRANAPLDALTQLMGLPGKVGIGGAQVWDAYQAGKLAEIRNYCEA
                     DVVNTYLVFLRYQLMRGALDDARHKAECDLVRSTLAKSAEPHWKEFLGKWKA"
     gene            725..2020
                     /gene="rlmD"
                     /locus_tag="AC158Q_12685"
     CDS             725..2020
                     /gene="rlmD"
                     /locus_tag="AC158Q_12685"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HIR3340674.1"
                     /translation="MQSLTIESLDQDGRGVARRDGKAIFIEGALPGEIVTYSSYRKKP
                     SYEQATLGTVLKESALRVAPRCPHFGVCGGCSLQHFDARAQVAVKQRVLEDSLWHIGR
                     VRPESMLPAIHGPAWGYRHRARFAVRYVPKKGGALVGFHEKRSSYIADMSRCEVLPPR
                     ISALIRPLRDLVGALSIRTRLPQIEVALGEGADVLVFRILQPLLEPDMDLLRRFADDH
                     GVKVYLQPGGPDTAGPMEPADYADLYYELSEFDLRIRFDPTDFTQINHAVNAVLVRRA
                     VALLGARPGERIADLFCGVGNFALAVARSGAQVVGVEGSAVLVGRARTNALLNGLADA
                     ASFHERDLYRGIADPLRGLGHFDRMLIDPPRDGAIGVAKSLPEDGPRRLVYVSCNPGT
                     LARDAAVLTQERGYRLSAAGVVNMFPHTAHVESIAVFDK"
     gene            complement(2224..2928)
                     /locus_tag="AC158Q_12690"
     CDS             complement(2224..2928)
                     /locus_tag="AC158Q_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HIR3340675.1"
                     /translation="MQPELQPNPAVYQAGAGGIALEQQRVLRNTYLLLALSLIPTAIG
                     AAVGTNLNLSFLRTSPILSFFLILAVFYGWIFAIERNKNSSLGLVLLLGFTFFMGLLL
                     GPLFQFVLGFKNGIQLVMMAAGGTAAVFFGLSGLAATTKRDFSFLSKFLFVGFFVIML
                     SVIANVFFASPLAHLVILAMFILFSSAAILWQVNAIVRGGETNYISATLTLYVSVYNL
                     FTSLLQLFGLLGGDRD"
     gene            3072..3161
                     /locus_tag="AC158Q_12695"
     tRNA            3072..3161
                     /locus_tag="AC158Q_12695"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3106..3108,aa:Ser,seq:tga)
     gene            complement(3188..3490)
                     /locus_tag="AC158Q_12700"
     CDS             complement(3188..3490)
                     /locus_tag="AC158Q_12700"
                     /inference="COORDINATES: protein motif:HMM:NF046299.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3340676.1"
                     /translation="MRPFERITNPREIRRRLRLNQQEFWSRIGVTQSGGSRYESGRGM
                     PKPVRELLRLVHIEGIPLNQAKGGDFEVVTYLKQANPRLYHSLRKAASRRNGRKKA"
     gene            3672..>4513
                     /locus_tag="AC158Q_12705"
     CDS             3672..>4513
                     /locus_tag="AC158Q_12705"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HIR3340677.1"
                     /translation="MALIVQKYGGTSVGSPERIKNVARRVARWTALGHQLVVVVSAMA
                     GETNRLIALARDIQPQPDPRELDVMVSTGEQVTIALLSMALMELGLKARSYTGGQVKI
                     LTDNAFTKARIVSIDEALIRQDLAGGCVVVVAGFQGVDEQGNITTLGRGGSDTTGVAL
                     AAALKADECQIYTDVDGVYTTDPRIVPEARRLKSVTFEEMLEMASLGSKVLQIRSVEF
                     AGKYRVRLRVLSSFEEEGEGTLITFEEDQKMEQASISGIAFNRDEAKITILGVPDRPG
                     IAYQ"
BASE COUNT          866 a         1355 c         1478 g          814 t
ORIGIN      
        1 acggcggcgg cttcgacctg ccggtgatgc actaccgcgg gctgatccac ggggtgaagg
       61 cgggccgcta ctgggacctg ggcgaggacg accgcgactt caagtggaac aattacatca
      121 gccgctacca ctcccgccat ctcgacctca tggatctcct cgcgctctac cagccgcgcg
      181 ccaacgctcc gctggatgcg ctcacgcagc tgatggggct gcccggcaaa gtcgggatcg
      241 gaggcgccca ggtgtgggac gcctaccagg cgggcaagct ggccgagata cgaaactact
      301 gcgaagccga cgtcgtcaac acttatctcg tatttctgcg ctaccagctg atgcgaggtg
      361 cgctggacga cgcgcggcac aaggccgagt gcgacctggt gcgctcgaca ctcgccaagt
      421 cagccgagcc gcactggaaa gagttccttg gcaaatggaa agcgtaaaaa caataatcga
      481 accgccaaga cgccaaggcc gccaagagaa tctatatatt tgtagatata tccctcatat
      541 gcctatatct atagagtcgt atatgccggc atatagattc agcagtgctg cgatgctgtc
      601 tattacaagt tgatctgaag tatttcttgg cggtcttggc ggttcgtttt gtaggttttc
      661 tgctttgcgt tagaatcgcg ccaccgtcat cgcggcgtac tttgactccg ccgcagcggg
      721 catcatgcaa agcctcacca tcgaatccct ggaccaggat ggtcgcggcg tcgcgcgccg
      781 cgacggcaag gccatcttca tcgaaggcgc actgccgggc gagatcgtca cctattcgtc
      841 ctatcgcaag aagccgagct acgagcaggc gaccctgggc accgtgctca aggaaagcgc
      901 attgcgtgtc gcgccgcgct gcccccattt cggcgtgtgc ggcggctgca gcctgcagca
      961 cttcgacgcg cgcgcgcagg ttgcggtcaa gcagcgcgtg ctggaggaca gcctgtggca
     1021 catcgggcgg gtgcggccgg agagcatgct gccggcgatc cacggccctg cgtggggtta
     1081 ccgtcaccgg gcgcgcttcg ctgttcgata cgtgccgaag aagggcggag cgctggtcgg
     1141 tttccatgaa aagaggagca gctatatcgc ggacatgagc cgttgcgagg tgctgccgcc
     1201 gcgcatatcc gccctgatcc ggccgctgcg cgatctcgtc ggcgcgcttt cgatccgcac
     1261 ccgcctgccg cagatcgagg ttgcgctggg ggagggagcc gacgtgctgg tgttccgaat
     1321 cctgcagccg ctgctcgaac ccgacatgga cttgctgcgg cggttcgcag acgatcacgg
     1381 cgtcaaggta tacctgcagc ccggcggccc cgacaccgcc ggcccgatgg agcctgcgga
     1441 ctacgcggac ctctactacg aactgagtga attcgacctt cgcatccgat tcgatcccac
     1501 tgacttcacg cagatcaatc acgccgtgaa tgccgtgctg gtgcggcgcg ccgtggccct
     1561 gctcggcgcg cggcccggcg agcgcatagc cgatctcttt tgcggcgtcg gcaattttgc
     1621 gctggccgtt gcgcgaagcg gcgcgcaggt cgtgggcgtg gaaggcagcg cggtgctggt
     1681 ggggcgggcc aggacgaacg cgctcctcaa cgggctggcg gatgcggcga gttttcacga
     1741 gcgcgatctt tatcggggga tcgcggaccc gctgcgcggc ctgggccact ttgaccgcat
     1801 gctgatcgac ccgccgcgcg acggggcgat tggcgtggcg aagtctctgc ctgaagacgg
     1861 gccccggcgc ctggtttatg tctcgtgcaa ccccggcacg ctggcgcgtg atgccgccgt
     1921 gctgacacag gagcgcggct accggctctc cgcggcggga gtggtcaaca tgttcccgca
     1981 cacggcgcac gtggaatcca tcgccgtctt tgacaaatga accgccaagg cgccaaggcc
     2041 gccaagaagc tcaaattacc attggaattt tcaatgcgtg tcgggcatca ctgcgggccc
     2101 gggtggcgcc ggaaccacac gtggcaatcg gcgcggtaca gaaaaacggc cgggcattcg
     2161 cccggccgtc attgattgct atctgctttc ttggcgcgct tggcggcctc ggcggttcag
     2221 ttgttaatcg cggtcgccgc ccagcaagcc gaagagctgc agcaggctcg tgaacaggtt
     2281 gtagaccgag acgtagaggg tcagcgtcgc ggagatgtag ttggtctcgc cgccgcgcac
     2341 gatggcgttg acctgccaca ggatagctgc cgaggagaac aggatgaaca tcgccagaat
     2401 caccaggtgc gccagcgggc tcgcgaagaa cacgttggcg atgacggaca gcatgatgac
     2461 gaagaagccc acgaacagga acttcgacag gaagctgaaa tcgcgcttgg tggtcgcggc
     2521 aaggcctgac agcccgaaga acacggccgc ggtgccgccc gccgccatca ttacgagctg
     2581 gatgccgttc ttgaagccga gcacgaactg gaacaacggc ccgagcaata gtcccatgaa
     2641 gaacgtgaag ccaagcagga ggacgagccc cagggagctg ttcttgttgc gctcgattgc
     2701 gaagatccag ccgtagaaga cggcgaggat caggaaaaac gagaggatcg ggctcgtgcg
     2761 caggaacgag aggttgagat tcgtgcccac cgcggcgccg atcgccgtcg ggatcagaga
     2821 aagcgcgagc agcaggtaag tattgcgaag aacgcgctgc tgttcgagcg cgattccgcc
     2881 ggcgccggcc tggtatacgg ccgggttcgg ttgcagttca ggttgcatgc ttcctccttg
     2941 aggatggact ctactgaggc ttaagactac ctaaaggaac caaaagtttc aatgggttag
     3001 agcaggtgtc agataccaag cgcttgataa cgcgtgtaat atagcccgcg ttctttgcga
     3061 gggtgcgtca gggaagggtg gcagagcggt tgaatgcacc ggtcttgaaa accggcatgt
     3121 ccgcaagggc atcgtgggtt cgaatcccac cccttccgcc aaaatgagtg cggatccctg
     3181 gtgcccgcta ggctttcttc cggccgttgc gccgggaagc ggccttcctc aggctgtggt
     3241 agaggcgggg attggcctgc ttgagatagg tgacgacctc gaaatcgccg cctttggcct
     3301 ggttgagggg aataccctcg atatggacca gcctcagcaa ttcgcgtacc ggcttcggca
     3361 tgccgcggcc gctttcgtag cgggagccgc cgctctgggt gacgccgatg cgcgaccaga
     3421 attcctgctg gttcaggcgc aggcggcgcc ggatttcacg cgggttggta atgcgttcga
     3481 atggtctcat gtcgaatgtc ctggttgata cgataatgga ccaccggcag tcgaataggt
     3541 tcatgccggg gccgaagccc gccgttggtt atgccgaagg catggctcat gcgtcgcggc
     3601 gtcccaggag cagcctcgtt ttcccccgaa aaatcaacta aaatacgcgg ttttttcgat
     3661 ctgcaccaac catggccctg atcgtccaga aatacggcgg cacctcagtg ggctcgcccg
     3721 agcgcatcaa gaatgtcgcc cggcgcgtcg cccgctggac cgcgctgggg caccagctgg
     3781 tggtggtcgt ctcggccatg gcgggcgaga ccaaccgcct gattgcgctc gcccgtgata
     3841 tccagccgca gcccgacccg cgcgagctcg acgtgatggt ttcgaccggc gagcaggtga
     3901 cgatcgcgct gctctcgatg gcgctgatgg agctgggact caaggcgcgc agctataccg
     3961 gcggccaggt gaagatcctc accgacaacg cctttaccaa agcgcgtatc gtgagcatag
     4021 atgaggccct gatccgccag gacctggccg gcggatgcgt cgtggtcgtc gccggcttcc
     4081 agggtgtgga cgagcagggc aacatcacca ctctggggcg cggcggttcg gacacgaccg
     4141 gggtcgcgct cgccgcggcg ctcaaggccg atgaatgcca gatctatacc gacgtggacg
     4201 gcgtttatac caccgacccg cgcatcgtgc cggaagcgcg gcggctcaag tccgtcacct
     4261 tcgaggaaat gctcgagatg gcgagcctgg gctccaaggt gctgcagatc cgctcggtcg
     4321 agttcgcggg caagtaccgg gtcaggctgc gggtgctctc cagcttcgag gaggagggcg
     4381 aaggcacgct gatcactttc gaggaagacc agaaaatgga acaggcaagc atttcaggca
     4441 tcgcgttcaa ccgcgacgag gcgaagatca ccatcctcgg cgtgcccgac cggccgggca
     4501 tcgcctacca gat
//
LOCUS       DBNGSG010000479         1945 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4060265, whole genome shotgun sequence.
ACCESSION   DBNGSG010000479 DBNGSG010000000
VERSION     DBNGSG010000479.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4060265"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="AC158Q_12710"
     CDS             <1..973
                     /locus_tag="AC158Q_12710"
                     /inference="COORDINATES: protein motif:HMM:NF014653.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HIR3340678.1"
                     /translation="EATELGERALRAIGFDAEQAGIITAHLVDAAMCGHRFAGLPRIL
                     TINEDPRTREPRTAVKVVHETEVSALMDGGNNVGYYAVHQAARLAIDKARRNRIAVVG
                     MYRSQLSGRNAYYLEMIARAGLVGIHLASAAPVVLPHGGAKAAFGTNPIGFGFPTGGD
                     PFIVDLGTAAMMRGEVILRSRLGLPLPDGTAIDARGLPTTDAAEALKGGISTFGGHRG
                     SALSFGIQAMCLLAGAARARGKVQDFAFFFVVFDPAILMPPEEFKAQIGELIEAVRAV
                     PPLDADHPVRIPSERAFRERERLRKEGIPVDRAVYDQLRALIAEGRK"
     gene            complement(1144..>1945)
                     /locus_tag="AC158Q_12715"
     CDS             complement(1144..>1945)
                     /locus_tag="AC158Q_12715"
                     /inference="COORDINATES: protein motif:HMM:NF039714.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6279 family lipoprotein"
                     /protein_id="HIR3340679.1"
                     /translation="YGQFDTYAAWTADEYFELDAKQKQEFHARFDRLHEWHRYEQLPD
                     YAAFLIAAKTRVQRGLTQEDVLWINSSLQARYRAIVRRGAPDAAVLLITVTPAQLEAL
                     QRKWERDNRRFVREHRLEDSADEQRRAATKRVLDRIRHWTGSLSNEQERKIAALAGEL
                     PVTHRLRHEDRLRRQHEFLELMKRRGDRVPFTETLPRWLLNWEEGRDPEFARVWAQWT
                     QKQADFYVAVDRMLTPQQREHVMQRLQKYADDFTALAQRPAPQAATAR"
BASE COUNT          301 a          642 c          657 g          345 t
ORIGIN      
        1 cgaggcgacg gagctgggcg agcgcgccct gcgggcgatc ggtttcgacg cagagcaggc
       61 ggggatcatc accgcgcacc tcgtggacgc ggcgatgtgc gggcaccgct tcgccggcct
      121 gccgcgcatc ctcacgatca acgaggatcc gcgcacccgc gagccgcgca cggcggtgaa
      181 agtcgtgcac gaaaccgagg tatcggcgct gatggacggc ggcaacaacg tcggctacta
      241 cgccgtccac caggcggcgc ggctcgcgat cgacaaggcg cgcaggaacc gcatcgccgt
      301 ggtcggcatg taccgcagcc agctctccgg ccgcaacgct tactacctgg agatgatcgc
      361 gcgggccggg ctcgtcggca ttcatctcgc gagcgccgcg ccggtggtgc tgccgcacgg
      421 cggcgcgaag gccgctttcg gcaccaaccc gatcggcttc ggtttcccga cgggcggtga
      481 cccgttcatc gtggacctgg ggaccgccgc gatgatgcgc ggcgaagtca tcctgcgctc
      541 gcggctcggc ctgccgctgc cggacgggac ggccatcgac gcacgggggc tgccgaccac
      601 cgatgcggcg gaagcgctga aaggcggcat ctccaccttc ggcgggcacc gcggctcggc
      661 gctgtcgttc ggtatccagg cgatgtgcct gctggcgggc gccgcgcgcg cgcgcggcaa
      721 ggtgcaggat ttcgccttct tcttcgtggt gttcgaccct gcgatcctga tgccgccgga
      781 agaattcaag gcccagatcg gagagctgat cgaggcggtg cgcgccgtgc cgccgctcga
      841 cgcggaccac ccggtgcgca tcccgtcgga gcgggcgttc cgcgagcgcg agcggctgcg
      901 gaaagagggg ataccggtcg accgggcggt ctacgaccag ctgcgcgcgc tgatcgccga
      961 aggcaggaaa tagacaggat tgaattcact caagaaccga acccgtcaaa accactaata
     1021 gacaggattt acaagattta caggattaag gcgatgttcg aatgctcttg attaaatctt
     1081 gttagtcccg tccatttacg atgttaacgg gtttttcgtt agatctcctg tgaatcctgc
     1141 ccgttaacgg gcggtggccg cctgcggcgc ggggcgctgc gcgagggcgg tgaagtcgtc
     1201 cgcgtatttc tgcagccgct gcatgacatg ctcgcgctgt tgcggcgtca gcatgcgatc
     1261 caccgcgacg tagaaatcgg cctgcttctg cgtccactgg gcccacacgc gcgcaaattc
     1321 ggggtcgcgg ccttcctccc agttgagcag ccagcggggc agcgtttccg taaacggcac
     1381 gcgatcgccg cgccgcttca tcaattccag aaattcgtgt tgccggcgca gccggtcctc
     1441 gtggcgcagc cggtgggtca ccggcagctc accggcgagc gcggcgattt tccgttcctg
     1501 ctcgttgctc aggctaccgg tccagtggcg gatgcgatcg agcacgcgct tcgtcgccgc
     1561 ccgccgctgc tcgtcggcgc tgtcctccag gcggtgttcc cgcacgaaac ggcggttgtc
     1621 cctctcccac tttcgctgca gcgcttcgag ctgggcgggc gtgacggtga tcagcagcac
     1681 ggccgcgtcg ggcgcgccgc gcctgacgat ggcacggtag cgcgcctgca ggctactgtt
     1741 gatccagagc acgtcttcct gcgtgaggcc tctctgcacc cgcgttttcg ccgcgatcag
     1801 gaatgccgcg taatccggca gctgctcgta gcgatgccac tcgtgcagtc ggtcgaagcg
     1861 cgcgtggaat tcctgcttct gcttcgcgtc gagctcgaag tactcgtcgg cggtccacgc
     1921 cgcgtaggtg tcgaactggc cgtac
//
LOCUS       DBNGSG010000480         1689 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3212772, whole genome shotgun sequence.
ACCESSION   DBNGSG010000480 DBNGSG010000000
VERSION     DBNGSG010000480.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3212772"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="AC158Q_12720"
     CDS             <1..397
                     /locus_tag="AC158Q_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340680.1"
                     /translation="MPLNELVVQRSKEQDLRRALREIREAIDGHKQASDDGRIQKRIG
                     ESGYPKKLEDLVNGVEDQKNPKKERIYFLRRIPRDPLDNEPGLAAADTWGRRSYANPP
                     DEPKEGDDVFDVYSRAPGTGINGRPYREW"
     gene            505..882
                     /locus_tag="AC158Q_12725"
     CDS             505..882
                     /locus_tag="AC158Q_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HIR3340681.1"
                     /translation="MNRREAAFTLIELLVVLAIVATLLMLAVPRYFSSLDRSKEAVLK
                     ENLYQMRDAIGKYYADKGRYPESVEALAAEKYLRRVPVDPITDSAATWVAVPPEDPQK
                     SGVYDVRSGAPGAAGNGTAYGEW"
     gene            882..1403
                     /locus_tag="AC158Q_12730"
     CDS             882..1403
                     /locus_tag="AC158Q_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010098173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HIR3340682.1"
                     /translation="MRRAAGFTYMTILFIVAIMGAGLALVGEVWHTAAVREREAELLH
                     VGNQYRRAIERYYLSGPRLYPRSLSDLLKDTRKPVTERYLRRLYPDPITGSADWGLIK
                     APDGGIMGVHSLSDEAPLKTAGFRARDRQFLGATQYREWKFAYEQQPASALQAPAPGG
                     AAPMSVTPPAPPR"
BASE COUNT          341 a          558 c          524 g          266 t
ORIGIN      
        1 catgcccctc aacgaactcg tcgtccagcg ctcgaaggaa caggatttgc gccgcgcgct
       61 gcgcgaaatc cgcgaggcca tcgacggcca caagcaggcc tcggacgacg gccgcatcca
      121 gaagcggatc ggcgagtccg ggtacccgaa aaaactcgag gacctggtca acggggtcga
      181 ggaccagaaa aacccgaaaa aggagcgtat ttacttcctc cgccgcattc cccgcgaccc
      241 gctcgacaac gaacccggcc ttgcggccgc ggacacctgg ggcaggcgca gctatgcgaa
      301 cccgccggac gaaccgaagg aaggcgacga cgtcttcgac gtctactccc gcgctccggg
      361 caccggcatc aacggccgcc cctaccggga gtggtgatga agcagtatcc aggatccagg
      421 atcggggatt cagcgcggtt attccgccag acggaaccct ccgaaccggg caccattcac
      481 gattcacgaa tcacgaatca cgccttgaat cggcgcgaag cagcgttcac gctgatcgag
      541 ctgctggtcg tgctcgcgat cgtcgccacg ctgctgatgc tcgccgtgcc gcgctatttc
      601 tccagcctcg accggtcaaa ggaggctgtg ctcaaggaga acctctacca gatgcgggac
      661 gcaatcggga agtactacgc cgacaagggc aggtatcccg aatccgtcga ggcgctcgcc
      721 gccgagaagt acctgcgcag ggtgccggtg gacccgatca ccgacagtgc agccacctgg
      781 gtggcggtgc cccccgaaga cccgcagaaa agcggcgtat acgacgtgag gagcggcgcg
      841 ccgggcgccg ccggcaacgg cacggcctac ggagagtggt gatgcggcgc gcggctggct
      901 ttacctacat gacgatcctc ttcattgtcg cgatcatggg cgcgggcctt gcgctcgtcg
      961 gcgaggtctg gcacacggcc gccgtgcgcg aacgggaggc ggagttgctg cacgtcggca
     1021 atcagtaccg cagggcgatc gagcgctatt acctgagcgg cccacgcctc tatccacgct
     1081 cgctgtccga cctgttgaag gacacgcgca agccggtcac cgagagatat ctgcgccggc
     1141 tgtatcccga cccgattacc gggagcgccg attgggggct gatcaaggcg cccgacggcg
     1201 gcatcatggg cgtccacagc ctctcggacg aagcgccgct caagaccgca ggattccggg
     1261 ctcgggaccg gcagttcctg ggagcaacgc agtaccggga atggaaattc gcctacgagc
     1321 aacagccagc ctcggcactt caggcgcccg cgcccggagg ggctgcgccc atgtcggtga
     1381 cgccacccgc gcccccgcgc taatggcggg caccctgccc agaacctatt tcaacaacca
     1441 aaaacaggat ttagccgcag ataaacgcag atacaaacca ggaataaatc agcaggttgg
     1501 tcttgattca atctgcgttc atctgcgttc atcggcggct tgatggccct tgacttgaat
     1561 gcaataaagc ggcgggctgc ggttgcccgc agccggccgg cgttgcgata tcccggtcgt
     1621 gtttcggcct cagtttccgc tcagcttccg ctcagtttcc aggatgtccc agcccgccgc
     1681 acatatcgg
//
LOCUS       DBNGSG010000481         3199 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4061540, whole genome shotgun sequence.
ACCESSION   DBNGSG010000481 DBNGSG010000000
VERSION     DBNGSG010000481.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4061540"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..1211
                     /locus_tag="AC158Q_12735"
     CDS             213..1211
                     /locus_tag="AC158Q_12735"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3340683.1"
                     /translation="MINKLSTFRCSLFSIAACLAAGAPAVFAQKTDSGAGFPSRPIRV
                     IVPFTPGGQPDIFSRMIGQYMAGSLGQQVVVDNRPGAGGMLGSRIVAEATPDGHTLLS
                     ISAAHSIGPSVRKLPYDTRRDFAGVSMAYNAAYLLVVPPTLGVKTTQDLIALAKSRPG
                     QLNLASAGRGSGTHFAGEVFKHAAGIDVVHVPYKGIPEATNDIMSGRVQLFMAPLASS
                     VPLIRDGRIRALGVTSPKRVDVLPDVPTIAEGGLKGFRWDSWGAIFAPAKTPRAVINK
                     LNRAITEALRQPETEKRMRALGAEPAPSTPEQLDRFVIQDLAMVAKIAKVAGIKPE"
     gene            1292..1792
                     /locus_tag="AC158Q_12740"
     CDS             1292..1792
                     /locus_tag="AC158Q_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340684.1"
                     /translation="MSHTIEQFAEACHKILKAEPNPAGRGKVSALLQEILRDQQFVST
                     YLKDETPERKILYEDPELGFCILAHHYRDAKVSPPHDHGPYWAIYGQAVGETEMSDYQ
                     LLEPAAPGKPGKVKKTRTYKLTPGVAHVYNEGDLHSPTRVRATKLIRIEGVNMEKVKR
                     LKFEAV"
     gene            complement(1866..2147)
                     /locus_tag="AC158Q_12745"
     CDS             complement(1866..2147)
                     /locus_tag="AC158Q_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340685.1"
                     /translation="MANNLFISFEFNRPDRTYDRVVHAIKSLSETIAEMHFAHWYVST
                     PLSAGQVCDRLKSLLDQTDKLIVVDTTNNKAAWINLGPDAVKRLQDQGY"
     gene            complement(2311..2964)
                     /locus_tag="AC158Q_12750"
     CDS             complement(2311..2964)
                     /locus_tag="AC158Q_12750"
                     /inference="COORDINATES: protein motif:HMM:NF025134.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HIR3340686.1"
                     /translation="MSVAGQELPVWPQGQKIELLAAPNMGVTDFEDCEQYHAGLSARI
                     LELERDPRYHHWIFKGGCGTKVRNPHAWGSAEADLIHARAMRLAAKALGVADVVVDEC
                     WANVYRKGDYCTPHSHLRSVASLIYLLDPGEPGIIDPASGRLCFCDPRIPFCCGHEAG
                     RATTLLMPELRPGSLLCFPSEYVHLVNPYEGERPRITMSWNLTLEKLPGSPADGWNR"
BASE COUNT          612 a         1053 c          971 g          563 t
ORIGIN      
        1 cgggctcctg tttcccgttt actcaaccaa ttccattgcg ccgcgggtcc aaacgcgcga
       61 atgtcatcaa gcttgcccgc ggagactgca aggtcgaagg tcccccgcta tactacccgg
      121 gtccggggag agaccgggat cgcggctgcc gcaagcagcg cggaggcaaa tccggcctca
      181 ggcaccataa atatacgagg aggcgtcacg acatgattaa taaattgtca accttcaggt
      241 gcagcctttt ttcgattgcc gcctgcctgg ccgccggtgc gccggcggtc ttcgcgcaga
      301 aaaccgattc gggggccggc ttcccgagcc ggccgatccg cgtcatcgtg ccatttacgc
      361 caggtggcca acccgacatc ttctcccgca tgatcggaca gtacatggcg ggatcccttg
      421 gacagcaagt cgtcgtcgac aatcgtcccg gagccggcgg catgctcggc tcgcgcatcg
      481 tcgccgaggc caccccggac ggccacacct tgctgtcgat atccgccgcg cactccatcg
      541 gcccttccgt gcggaagctc ccgtacgaca cgcgcaggga ttttgccggc gtctcgatgg
      601 cctacaacgc cgcctatctg ctggtggtgc cgcccaccct gggcgtgaag acgacgcagg
      661 atctcatagc gctcgccaag tccaggccgg gccagttgaa cctcgcctcg gccggccgcg
      721 gtagcggcac ccatttcgcc ggggaggtgt tcaagcatgc cgccggcatc gacgtcgtgc
      781 acgtgcccta caagggcatc cccgaagcga cgaatgacat catgtccggg cgcgtgcagc
      841 tcttcatggc gccgctcgcg agctcggtgc cgctgatccg ggatggcagg atacgcgcgc
      901 tcggggtcac ctcgcccaag cgcgtcgacg ttcttcccga cgtgccgacc atcgcggaag
      961 gcgggctcaa gggtttccgc tgggactcct ggggggcgat attcgcgccg gcgaagacgc
     1021 cgcgcgcggt catcaacaag ctgaaccggg cgatcacgga agcgctgcgt cagcctgaaa
     1081 ccgaaaagcg catgcgcgcc ctgggggcag agccggcgcc ctccacgccc gagcaactcg
     1141 acagattcgt catccaggat ctggccatgg tcgcgaaaat cgctaaggtc gcgggcatca
     1201 agccggagta accggcccga tccgcagttc actgaagccc ggtattgagt agaattgcca
     1261 ccgaacgctt tatcacttac aggaggggtt catgagccac accatcgagc agttcgccga
     1321 ggcctgtcac aagatcctca aggccgaacc caatccggcc ggccgcggca aggtcagcgc
     1381 cctgcttcag gagatcctca gggaccagca gttcgtctcc acctatctca aggacgagac
     1441 acccgagcgc aagattttgt acgaggatcc ggaactgggc ttctgcatcc tcgcgcacca
     1501 ctaccgcgat gccaaggtga gcccgccgca cgatcacggc ccgtactggg caatctacgg
     1561 ccaggccgtc ggcgaaaccg agatgtccga ttaccagttg ctggagccgg cagcgcccgg
     1621 caaaccgggc aaggtgaaga aaacccgcac ctacaaactt acgcccggcg tggcgcacgt
     1681 ctacaacgaa ggcgatctgc actccccgac aagggtccgt gcgaccaagc tgatccgcat
     1741 cgagggcgtc aacatggaaa aagtgaagcg gctgaagttc gaggccgtat aaccgcgccc
     1801 ggcctccgcc ggaggtggaa cggggcagcc cgggcggcct cctgttactg ccatctcgtg
     1861 ggcgctcagt aaccttgatc ctgcaggcgc ttgacggcgt ccgggcccag gttgatccag
     1921 gccgccttgt tgttcgtggt atccacgacg atcagcttgt cagtctgatc cagcagcgac
     1981 ttcaaccggt cgcagacttg cccggcggac agcggggtgc tcacgtacca atgcgcgaag
     2041 tgcatctccg caatggtctc gctgagggac ttgatcgcgt ggaccacccg gtcgtaggtc
     2101 cggtccggac gattgaattc gaacgaaatg aataggttat tagccatgct gtcctgaacg
     2161 atacggctgc tcgaccgggg acatattgat tataatcaag tccgggcatt tttctgccaa
     2221 tcagcgggat cactaagggg gccgggaaca acgcggccgc aactctgccc ctacgtttcc
     2281 atcgtcaccg cgcggcggcg attttcccga tcaacggttc cagccgtcgg cagggctgcc
     2341 gggcagcttt tcaagcgtga ggttccacga catcgtaata cgcggccgct cgccttcgta
     2401 cgggtttacc agatggacat actcgctcgg aaaacacaga agcgaccccg ggcgcagctc
     2461 aggcatgagc aacgtcgtcg cgcggcccgc ctcgtggccg cagcagaacg ggatgcgggg
     2521 atcgcaaaag cacagccggc cgcttgcggg gtcgatgatc ccgggttcgc cggggtcgag
     2581 caggtaaatc aggctggcaa cggaacgcag gtggctgtgg ggcgtgcagt agtcgccctt
     2641 gcggtagacg tttgcccagc actcgtccac gaccacgtcc gcgacgccca gcgccttggc
     2701 cgcgagccgc attgcccgcg cgtggatgag gtccgcttcc gcgctgcccc acgcgtgcgg
     2761 gttgcgcacc ttggtgccgc agccgccctt gaatatccag tggtgatacc gcggatcccg
     2821 ttccagttcc aggatcctgg cagagagccc cgcgtgatac tgctcgcaat cctcgaaatc
     2881 cgttacgccc atgttgggag ccgcaagcag ctcgatcttc tgcccctgcg gccacaccgg
     2941 cagctcctga cccgctacgg acaccgattg cggaatcatt cagcgaaggg atacccgcag
     3001 gagcgggtac gcccttcatc tcctcgattt aatgggccgc tgcggtcttg ccgagcagcc
     3061 acttagttat aaatacggga tccgcgccgc cgatcccggg catacctgac gaccaggcta
     3121 gtttactgct tttgcgcgga tctcgcccca gcctggctgg cgaacaggcg caaatatgga
     3181 ttgagtccca tacgcctcc
//
LOCUS       DBNGSG010000482        10768 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3382849, whole genome shotgun sequence.
ACCESSION   DBNGSG010000482 DBNGSG010000000
VERSION     DBNGSG010000482.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 10768)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10768)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10768
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3382849"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="AC158Q_12755"
     CDS             complement(<1..762)
                     /locus_tag="AC158Q_12755"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR3340687.1"
                     /translation="MKLKTLLGSRPLGYAAATLLVAAISGGALVVSSRTGQAVSATPA
                     LTAPGINGNHAAGPEALLFRTLHEVSNKRLDVAISEIDRIITTYPNFRLAHLIKGDLL
                     LARSRPLTTVGNAPGAPKDLLEDLREEARARLARIQYQRPVDLVPRYLVQMQPEQRHA
                     LVVDTAKSTLYVFENRGGEARYVADYYITIGKNGIDKYKEGDKKTPLGVYHVTGNIPR
                     EKLSATYGKASELYGNGALPISYPNEWDRRQGRAGY"
     gene            complement(759..1790)
                     /locus_tag="AC158Q_12760"
     CDS             complement(759..1790)
                     /locus_tag="AC158Q_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase Cds6 family protein"
                     /protein_id="HIR3340688.1"
                     /translation="MFFPAVRFIALAAALLLVLFPVAPAIAQSDELLETSQLLKQGQL
                     DRALERVDAYLKTRPKDARGRFLKGLILAEQNKPNDAIKVFTELTQEFPELPEPYNNL
                     AVLYASQGQYDKARASLEMAIRTHPSYATAHENLGDIYAKMASQAYDKALQLDKANTP
                     LQTKLNLIKDLFSSNSRPPKVSAARPDTAKVAVATPAPKKSPATVPPAPAKTVEPASK
                     PAPAREPAKPAQNPDEVLKTLNQWAKAWAGNDIDGYLGHYAADFQTPKGMARSDWEAQ
                     RKARIAKPRKIQVNIESPKVMFNGNGRVSVTFRQHYQSGALKVDSTKTLVMVKAGDKW
                     LIQQERVGG"
     gene            1978..3360
                     /gene="cysS"
                     /locus_tag="AC158Q_12765"
     CDS             1978..3360
                     /gene="cysS"
                     /locus_tag="AC158Q_12765"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011614939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HIR3340689.1"
                     /translation="MLKIYDTLARDKRPFVPIEEGRVRMYVCGMTVYDYCHLGHARVM
                     VVFDLVRRWLRAAGYSVTYVRNITDIDDKIIKRAQENGEPVEALTARFIQAMNEDADA
                     LGIERPDAEPRATGHVPGMLEMIGLLERRGLAYQAPNGDVNYPVRKFPDYGRLSGKSL
                     DDLRAGERVEVVPGKQDPLDFVLWKKAKEGEPSWDSPWGKGRPGWHIECSVMSSALLG
                     AHFDIHGGGQDLQFPHHENEIAQSEGAHQSKFVNYWMHNGFVRVMKGEMSEKMSKSLG
                     NFFTVREILKQYDPEVVRFFIARAQYRSPLSYSDGHLDDAKHALTRLYTALKGHPAKA
                     GEVDWNNSHAQRFREAMNDDFNTPEAMAVLFDLANEVNKTRSTDIAGLLRSLAGILGL
                     LQRDPTSFLQALPAGEGFGPERIEALIAARVAARKAKNFAEADRIRQELLDGGVVLED
                     GPKGTIWRRS"
     gene            3448..4599
                     /locus_tag="AC158Q_12770"
     CDS             3448..4599
                     /locus_tag="AC158Q_12770"
                     /inference="COORDINATES: protein motif:HMM:NF024771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR3340690.1"
                     /translation="MGKIAEVRTHPVSIPLKQTLWTAHEELKDSSTILVEVRTEDGIT
                     GVGQIHGSPQKAICEWVARLADIIRGMEATATVAAWEKMFSLTSPRPNGMGGRDGLPP
                     PLARSARPHIMAAIAGIDIALWDIRGKAAGLPVYRLLGAEKRPIFTYATGGYYQLGAP
                     DKVYADELARFVEAGFRAVKLKTGGGTAAEEAKRVRLVREAIGKDVSLMLDMNAPYDI
                     DECIAFARAVEPLDIFWLEEPLHWYLQPADFLRLAQATPIPLAHGERELTRFTVRDFI
                     ANGSIRYVQFDSTRYAGFTEALRVAQLAEQHGVMIAPHTVPELHAHLVMAFPRCGFAV
                     ESHGNAQRDPVSHGLYREHVHTKDSHVYLNDKPGFGVEVDWDFVNKHRA"
     gene            4660..5625
                     /gene="corA"
                     /locus_tag="AC158Q_12775"
     CDS             4660..5625
                     /gene="corA"
                     /locus_tag="AC158Q_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HIR3340691.1"
                     /translation="MLINCAAYQNGKKLGDIRKDEIHEYLNRSGCFVWVALKDGTPEE
                     LAELKQQFGLHELAVEDAHHGHQRPKIEEYGDSLFMVLHMIEPVGGELRVGELSLFVA
                     SNYVLSVRSRSEQGFASVRARTEREPHLLKHGAAFVFYALIDTVVDRYFPVLDAVEDE
                     LESVESRMFDGSPGRENIEALYAIKQKLMVLKHAVGPLHEAVGRLYGGRVPAVCAKTE
                     EYFRDVGDHLLRLNQSIDSLRDMVITALSVNLSMITIGENETVKRLASYAALVAVPTM
                     IAGVYGMNFKHMPELDWAWGYPFAVGLMVAIDAYLFWRFRKAKWL"
     gene            complement(5651..6412)
                     /locus_tag="AC158Q_12780"
     CDS             complement(5651..6412)
                     /locus_tag="AC158Q_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HIR3340692.1"
                     /translation="MIMLPIYLTVFGLLTALVAYSFRILREYERGVVFMLGRFWKVKG
                     PGLIIVVPFIQQMVRVDLRTRVLDVPPQDVISRDNVSVKVNAVVYYRVMDPERAIIQV
                     ENFNQATSEIAQTTLRSVLGQHELDEMLAEREKLNSDLRSLLDEATDAWGIKVSNVAL
                     KHVDLNETMVRAIAKQAEAERLRRAKIIDAEGEQQAAERLAQAGDILARRPQAMQLRY
                     LGALLNIAGERSNTIVFPLPIELLSAFRPQPEHKE"
     gene            complement(6417..7787)
                     /locus_tag="AC158Q_12785"
     CDS             complement(6417..7787)
                     /locus_tag="AC158Q_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR3340693.1"
                     /translation="MLRALILFAALLSGGAAAESARPVITFEIKGAIGVAATMQMEAA
                     LVRARQENAALVVMNLDTPGGLVTSTRDIIQAMLASPVPIAVYVSPSGARAASAGTYM
                     AYAAHVSAMAPGTHLGAATPVQMGPPGGTPGSPAPAKRDGEPGRKDAPTDGQSAMERK
                     VLNDAIAYLRSLAQLRGRNAAWAEKAVRDAATLTAEEAVKARVVDFLAADLAELLARA
                     DGRRVKAAGVEQRIATKGAETIAVEPDWRMRVLGVLTNPNIAFLLLLAGVYGILFEFW
                     NPGAVVPGVIGGISLLLALAALTVLPVNYAGLALLLLGIALMTVEALTPGIGALGIGG
                     LVAFIAGGVFLFDPAGADIAFRVTWPVLAVTAGASAGFFLFALGYALRSRRRKVVSGG
                     EEMIGMAGRVVAWDGLAGRVRLHGEEWRALADAPLAPGDEISVVSRDGLTLTVRPSTT
                     TGETHA"
     gene            complement(7964..9085)
                     /gene="dnaJ"
                     /locus_tag="AC158Q_12790"
     CDS             complement(7964..9085)
                     /gene="dnaJ"
                     /locus_tag="AC158Q_12790"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020700876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HIR3340694.1"
                     /translation="MAKRDYYEVLGVNRDASEEDIKKSYRKLAMKWHPDRNPDNPKAE
                     DRFKEAKEAYEVLSDSQKRAAYDQFGHAGVDPAAAAGAGAGAGFGNFADAFGDIFGDI
                     FGGGRGRSNVYRGADLRYNLEVSLEEAARGTETRIRIPAMEECVTCHGSGAKPGTQPT
                     TCPNCQGHGQVRMQQGFFSIQQTCPRCHGSGKIVASPCATCGGAGRIKQHKTLSVKIP
                     AGVDEGDRIRLSGEGEAGVNGGPPGDLYVVIHLKPHPVFTRDHNDLHCEMPISFTTAA
                     LGGEIEIPTLDGYAKIKVPAGTQAGQMFRLRSKGIKGVRSSGHGDLICHVAVETPVNL
                     TARQTELLAELEAINQKDGGRHNPRAKSWMEKVREFFAQ"
     gene            complement(9194..>10768)
                     /gene="dnaK"
                     /locus_tag="AC158Q_12795"
     CDS             complement(9194..>10768)
                     /gene="dnaK"
                     /locus_tag="AC158Q_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIR3340695.1"
                     /translation="KMKKTAEDYLGEPVTEAVITVPAYFNDSQRQATKDAGRIAGLVV
                     KRIINEPTAAALAFGMDKQEKKDRKIAVYDLGGGTFDISIIEIANVEGEHQFEVLSTN
                     GDTFLGGEDFDQRLIEHVCDEFKKDSGIDLKKDILALQRLKDAAEKAKIELSSGAQTE
                     INLPYITADQSGPKHLAVKVTRAKFESLVEDLIERTIEPCRMAIKDAGIKAADISDVI
                     LVGGQTRMPKVQDKVKEFFGREPRKDVNPDEAVAVGAAVQAGVLKGDVKDVLLLDVTP
                     LSLGIETLGGVMTKLIQKNTTIPTKAQQVFSTAEDNQTAVTIHVLQGEREMARGNKSL
                     GQFNLTDIPPAPRGMPQVEVMFDIDANGILHVSAKDKATGKENKIKIQASSGLTEQEI
                     QRMVKDAEENAEEDRKAHELVEARNQCDHMVHSVKKSLKDYGDKVDADEKAKIEAALK
                     EAEDVLKSDDKAAIEAKTQALAQAAHKLAETLYKEEQAKQQAQQAGGEAKADDGAKKD
                     DGNVVDAEYTEVKDKK"
BASE COUNT         1703 a         3451 c         3545 g         2069 t
ORIGIN      
        1 gtagcccgca cggccctggc ggcggtccca ctcgttaggg tagctgatcg ggagcgcccc
       61 gttgccatac agctcgctcg ccttgccgta agtggcggag agcttctcgc gtggaatgtt
      121 cccggtcacg tggtagacgc cgaggggggt tttcttgtcg ccttccttgt acttgtctat
      181 tccatttttc ccgatagtga tgtagtagtc cgcgacgtaa cgtgcctcgc cgccgcggtt
      241 ctcgaatacg tacagcgtgg attttgccgt gtccaccacc agcgcatgac gctgctccgg
      301 ctgcatctgc accaggtagc gcggcacgag atcgacgggg cgctggtact ggatgcgcgc
      361 caggcgcgcg cgcgcctcct cgcgcaggtc ctcgagcaga tctttgggcg cgcccggcgc
      421 gttgcccacc gtggtgagcg gccgggagcg ggccagcagc aggtcgccct tgatgaggtg
      481 cgccagcctg aaattggggt aggtggtgat gatcctgtcg atctcggaga tggcgacatc
      541 gaggcgcttg ttgctgactt catgcagggt ccggaacaga agggcttccg ggcccgcggc
      601 gtgattgccg ttgatgcccg gtgccgtgag agcgggggtt gcggacacgg cctggccggt
      661 acggctgctc acgacgagcg ctccgccgct gatggctgcg acgagcagcg tcgcggccgc
      721 gtagcccagc ggacgcgagc cgagcagcgt tttcagcttc acccgccgac cctttcctgc
      781 tggatgagcc acttgtctcc cgcctttacc atcaccagcg tcttggtgct gtccaccttg
      841 agcgcgcccg actggtagtg ctggcggaag gtcaccgaga cgcgaccgtt gccgttgaac
      901 atcaccttcg gtgactcaat attcacctgg atcttgcgtg gtttggcgat gcgcgccttg
      961 cgctgggctt cccagtcgct gcgcgccatg cccttgggcg tctgaaaatc cgccgcgtag
     1021 tgccccaggt agccgtcgat atcattgccc gcccaggctt ttgcccactg gttgagcgtt
     1081 ttcagcactt cgtccggatt ctgcgcgggt ttggcgggct ctctggccgg ggcaggcttg
     1141 gatgccggtt ccaccgtttt ggccggcgcc ggtggcactg tggccggcga tttcttcgga
     1201 gctggcgttg cgaccgcgac cttggccgta tccggcctgg ctgcggaaac cttcggcggg
     1261 cgcgagttgc tcgagaacag gtctttgatg aggttcagct tggtctgcag gggcgtattg
     1321 gccttgtcga gctgcagtgc cttgtcatag gcctggctcg ccatcttggc gtaaatgtcc
     1381 ccgaggttct cgtgcgcggt ggcatagctc ggatgggtac ggatcgccat ttcgagcgaa
     1441 gcgcgcgcct tgtcgtactg gccctgggat gcgtagagca ctgccaggtt gttgtacggc
     1501 tcgggcagct cgggaaattc ctgagtgagt tcggtgaaaa ccttgatcgc gtcgttgggt
     1561 ttgttctgtt cggcgagtat cagccccttg agaaagcgcc cgcgcgcgtc tttcggccgg
     1621 gtcttgagat aagcatcgac gcgttcgagc gcccggtcga gttgtccctg cttcagcagc
     1681 tggctggttt ccaggagttc gtcgctttga gcgatcgccg gcgcgacagg aaaaagcacg
     1741 agcaacagcg ctgcggcgag ggcgatgaag cggacggcgg ggaaaaacat tgtgctatac
     1801 ttttccgata atttcagtgg cttagtgcct gaatcctcga aattttacca agaacaccgg
     1861 gtaattaaaa cagcgcgttc cagcctcgcc gccggcatgc tcccgccccc ctgaccgatg
     1921 cctgcccggg cggcacaatc atcgcccgcg gcgccctgac gggcgattcc cgcagccatg
     1981 ctgaaaatct acgacacgct cgcccgagac aagcggccat tcgtgcccat cgaggagggc
     2041 agggtccgca tgtacgtctg cggcatgacg gtctacgact actgccatct cgggcacgcg
     2101 cgggtgatgg tggtgttcga cctggtgcgg cgctggctgc gcgccgccgg ctactcggtg
     2161 acctacgtgc gcaacatcac cgacatcgac gacaagatca tcaagcgtgc gcaggagaac
     2221 ggagagccgg tcgaagcgct gaccgcgcgc ttcatccagg caatgaacga ggacgccgat
     2281 gcgctgggca tcgagaggcc ggatgccgag ccgcgcgcca ccggacatgt gccgggcatg
     2341 ctcgagatga tcgggttgct cgagcgcaga gggctcgcct accaggcccc caatggcgac
     2401 gtcaattacc cggtccgaaa atttcccgat tacggcaggc tctcgggcaa atcgctcgat
     2461 gacctgcggg ccggtgaacg cgtcgaggtg gtcccgggga agcaggatcc gctcgatttc
     2521 gttttgtgga aaaaagcgaa ggagggtgag ccgtcgtggg actcgccgtg gggcaagggg
     2581 cgccccggct ggcacatcga gtgctcggtg atgtcgagcg cgctgctcgg cgcgcatttt
     2641 gacattcacg gcggcggtca ggacctgcag tttccgcatc acgagaacga gatcgcgcag
     2701 tcggaaggcg ctcaccagtc aaagttcgtc aattactgga tgcacaacgg cttcgtgcgc
     2761 gtgatgaagg gggaaatgag cgagaagatg tcgaagtcgc tcggcaattt cttcacggta
     2821 cgcgagatcc tcaaacagta cgacccggag gtcgtgcgct ttttcatcgc gcgcgcgcaa
     2881 taccgcagcc ctctcagcta ctcggatggc catctcgatg acgccaagca tgcgttgacc
     2941 cgcctctaca cggctctcaa aggccatcca gccaaggcgg gcgaggtgga ctggaacaat
     3001 tctcacgcac aacgcttccg cgaagccatg aacgatgact tcaacacgcc ggaagccatg
     3061 gcggtgctgt ttgatcttgc caacgaggtc aacaagaccc gatcgaccga catcgcgggc
     3121 ctgctcaggt cgttggcggg gatattgggg ctgctgcagc gcgaccccac gtccttcctg
     3181 caggcgctgc cggcgggcga ggggttcggc ccggaacgga tcgaggcatt gattgccgcg
     3241 cgcgtggcgg cgaggaaagc gaagaacttc gccgaagccg accgcatacg ccaggaacta
     3301 ctggatggtg gcgtcgtcct cgaggacggc cccaagggga cgatctggag gcgcagttga
     3361 tggttcgttg ccggtgaccc ggcggccggt atgccccgcg taccggcgtg gttgctgaac
     3421 gatgggcgac gattggagga gggatagttg ggcaagatcg cggaagtgag gacgcacccg
     3481 gtttcgatac cgctcaagca aacgctgtgg acggcgcacg aggaactgaa ggattccagc
     3541 acgatcctgg tggaggtgcg caccgaagac gggatcacgg gcgtcggcca gatccacggc
     3601 tctccgcaga aggcgatctg cgagtgggtg gcgcggctgg cggacatcat tcgcgggatg
     3661 gaggctaccg cgaccgtcgc ggcgtgggag aagatgttct ccctgacttc gccgcgcccg
     3721 aacggcatgg gcggacgcga tggcctgccc ccgccgctcg cgcgcagcgc gcgcccgcat
     3781 atcatggcgg cgatcgccgg tatcgacatc gcgctgtggg acattcgcgg caaggccgcg
     3841 ggcctgcctg tttaccgcct gctcggcgcg gagaaacggc cgatttttac ctacgccacc
     3901 ggcggctact accagctggg cgcgcccgac aaggtctacg cggacgagct cgcccgtttc
     3961 gtcgaggccg ggttccgtgc agtcaagctg aagaccgggg gcggcacggc ggcggaggaa
     4021 gcgaagcgcg tgcgcctggt gcgcgaggcg atcgggaagg acgtatcgct catgctcgac
     4081 atgaatgccc cgtacgacat cgacgaatgc atcgccttcg cgcgcgcggt ggagccgctg
     4141 gacatcttct ggctggagga gccgctgcac tggtacctgc agcccgcgga tttcctgcgg
     4201 cttgcccagg caacgcctat cccgctcgct cacggcgagc gcgagctcac ccgtttcacg
     4261 gtgcgcgatt tcattgccaa cggctcgatc cgctacgttc agttcgactc gacgcgctac
     4321 gcaggcttca ccgaagcgct gcgagtcgcg cagctggccg agcagcatgg cgtcatgatc
     4381 gcgccccaca ccgtgccgga gctgcacgcc cacctggtca tggcgttccc gcgctgcggc
     4441 ttcgcggtgg aatcgcatgg caacgcccag cgcgacccgg tttcccacgg cctctaccgc
     4501 gagcacgtac atacgaagga cagccacgtt tacctcaacg acaagcccgg cttcggcgtc
     4561 gaggtggact gggatttcgt gaacaagcac cgcgcttgac atgccccatc catcgcccgg
     4621 accgttttat cattgagccc atagacgagg atccggcaca tgctgatcaa ttgcgccgcc
     4681 taccagaatg ggaagaagct gggcgacatc cgcaaggacg agattcacga gtacctgaac
     4741 cggtccggtt gcttcgtgtg ggtggcgctc aaggatggga caccggagga gcttgccgag
     4801 ctgaagcagc agttcggcct gcacgaactg gcggtcgagg acgcgcacca tggccaccag
     4861 cggcccaaga tcgaggagta cggcgactcg ctgttcatgg tgctgcacat gatcgagccc
     4921 gtcggcggcg agttgcgcgt cggcgagctg agcctcttcg tcgccagcaa ttatgtgctg
     4981 tccgtgcgca gccgctccga gcagggcttc gcctccgtgc gcgcgcggac cgagcgcgaa
     5041 ccgcacttgt tgaaacacgg cgcggcgttc gtgttctacg cgctgatcga cacggtggtg
     5101 gaccgttatt ttccagtgct ggatgccgtg gaggacgagc tcgagagcgt ggaatcgcgc
     5161 atgttcgacg gcagcccggg gcgcgaaaac atcgaggcgc tctacgcgat caagcagaag
     5221 ctgatggttc tgaagcacgc ggtggggccg ctgcacgagg cggtcggcag gctctacggc
     5281 gggcgcgtgc cggcggtctg cgccaagacc gaggagtact tccgcgacgt gggcgaccac
     5341 ctgttgcgcc tgaaccagtc catcgacagc ctgcgcgaca tggtgatcac ggccctgtcc
     5401 gtcaacctgt ccatgatcac catcggcgag aacgaaacgg tcaagcggct ggcctcttac
     5461 gccgcgctgg tcgcggtgcc gacgatgatt gccggcgtct acggcatgaa cttcaagcac
     5521 atgccggaac tggattgggc atggggctat ccgttcgcgg tgggcctgat ggtggcgata
     5581 gacgcctacc tgttctggcg cttccgcaag gcaaagtggc tctaaccgta ctcccgcgcc
     5641 cccgtgccgc tcattccttg tgttccggct gtggccggaa agccgagagc agctcgatgg
     5701 ggagcgggaa tacgatggta ttgcttctct cgcccgcgat attgagcagc gcgccgaggt
     5761 agcgcaattg catggcctgc gggcgccgcg cgaggatatc gcccgcctgt gccagccgct
     5821 cggccgcctg ctgctcgcct tcggcatcga tgattttcgc gcggcgcagc cgctcggcct
     5881 cggcctgttt cgcgatcgcc cgcaccatgg tttcgttcag gtccacgtgc ttgagcgcga
     5941 cgttggaaac cttgatgccc caggcgtcgg tggcttcgtc gagcaggctg cgcaggtcgc
     6001 tgttcagttt ctctcgctcg gcgagcattt cgtcgagttc gtgctggccg agcaccgagc
     6061 gcagcgtggt ctgggcgatc tcgctggttg cctggttgaa attctcgacc tggatgatgg
     6121 cccgctccgg gtccatgacg cggtaataga cgaccgcatt gaccttgacc gatacgttat
     6181 cgcgcgagat gacgtcctgc ggcggcacgt ccagcacgcg ggtgcgcagg tcaacccgca
     6241 ccatctgctg tatgaacggc accacgatga tcaggccggg ccccttcacc ttccagaatc
     6301 ggccgagcat gaagacgacc ccccgctcgt attcgcgcag gatgcgaaac gagtatgcga
     6361 ccagtgcggt cagcaggccg aacacggtga ggtagatcgg cagcattatc atggattcag
     6421 gcatgagttt ctcccgtcgt tgtggaaggt cgcaccgtga gcgtcaggcc gtcccggctc
     6481 accacgctga tctcgtcgcc gggcgcaagc ggggcgtcgg ccagcgcgcg ccactcttcg
     6541 ccgtgcaggc ggactctgcc cgcgagcccg tcccacgcga ccacgcggcc agccatgccg
     6601 atcatttcct ccccgccgct taccaccttg cggcggcgag agcgcagcgc gtaacccagg
     6661 gcaaacagaa agaaccccgc gcttgccccc gcggtcacgg caagcaccgg ccatgtcacg
     6721 cggaacgcga tgtccgcgcc tgcgggatcg aaaaggaaaa cgcctcctgc aatgaacgcg
     6781 actagaccgc caatgcccag cgcgccgatg cctggcgtga gcgcttctac ggtcatcagg
     6841 gcaattccca gcagcaggag cgcgagcccg gcgtagttga ccggcagcac ggtgagggcc
     6901 gccagcgcga gcagcaggct gattccgccg atgacgcccg gcaccacggc cccgggattc
     6961 cagaattcga acaggatgcc gtagacgccc gccagcagca gcaggaaggc aatgttcgga
     7021 ttggtgagca cgcccaggac gcgcatgcgc cagtccggct cgacggcgat tgtctcggcg
     7081 cctttagtcg ctatccgctg ttccacgccg gcggccttga cgcgacggcc atcggcgcgc
     7141 gcaagcaatt ccgcaaggtc ggcagcgaga aaatcgacga cgcgcgcctt aaccgcctcc
     7201 tccgcggtga gcgtggccgc atcgcgcacg gctttctccg cccacgccgc gttgcggccc
     7261 cgcaattgcg ccaggctccg caggtaggcg attgcgtcgt tcagcacctt gcgctccatg
     7321 gcgctctgac cgtcggtggg ggcgtccttc ctgccaggtt cgccatcgcg ctttgccggg
     7381 gcgggggagc cgggcgtgcc tcccggggga cccatctgga ccggcgtcgc cgcgcccagg
     7441 tgcgtgccgg gcgccatcgc ggagacgtgc gccgcatagg ccatgtaggt tccggcgctg
     7501 gccgcacggg cgccgctggg cgagacgtag actgcaatcg gaaccggcga ggcgagcatt
     7561 gcctggatga tgtctcgggt cgatgtgacg agaccgcccg gcgtatcgag gttcatgacg
     7621 acgagcgcgg cgttttcctg gcgtgcccgt acaagcgccg cttccatctg catcgtcgcg
     7681 gcaacgccga ttgcgccctt gatctcgaac gtgatgaccg ggcgcgcgga ttccgcagcc
     7741 gcccccccgc tcagcagcgc agcgaaaaga atcagggccc gcagcaagca agcgcttccc
     7801 gcgaggcctg tccgggctcc aggtgcggtc atcctgtgtg cctcctcacc ggccgcgctg
     7861 cgtcacgcgg cctcgcgcag aatactgaac ggcattatgc gcctgaatcg cgcgggggaa
     7921 gggccagcag cgaagccggg tgtgtcctgg atggttcgcg ggtttactgg gcgaaaaact
     7981 cccgtacctt ttccatccac gatttcgcgc gtgggttgtg ccgtccgccg tccttctggt
     8041 tgatcgcctc gagctcggca agcagctccg tctggcgcgc ggtgaggtta accggcgtct
     8101 cgaccgcgac gtggcagatc agatcgccgt ggccggacga acgcacgccc ttgatgccct
     8161 tgctgcggag gcgaaacatc tggccggcct gcgtgcccgc cggtaccttg atcttggcgt
     8221 agccatcgag cgtcggaatc tcgatttcgc cgccgagcgc cgcggtggtg aagctgatcg
     8281 gcatctcgca gtgaaggtcg ttgtggtcgc gtgtgaaaac cgggtggggc ttgaggtgga
     8341 tcacgacgta gagatcgccc ggcgggccgc cgttcacgcc ggcttcgccc tcgcccgata
     8401 gccggatgcg gtcgccttcg tccacgccgg cgggaatctt caccgacagc gtcttgtgct
     8461 gcttgatgcg cccggcgccg ccgcacgtgg cgcagggcga ggccacgatc ttgccgctgc
     8521 cgtgacagcg cgggcaggtc tgctggatcg agaaaaatcc ctgctgcatg cgcacctggc
     8581 cgtggccctg gcagttggga caggtcgtgg gctgcgtgcc gggcttggcg ccggagccat
     8641 gacaggtgac gcactcttcc atcgcgggga tgcggatgcg cgtctcggtg ccgcgcgcgg
     8701 cctcctccag ggacacctcg aggttgtagc gcaggtccgc cccgcggtac acgttcgagc
     8761 gcccgcgccc gccgccaaag atgtcgccga agatgtcgcc gaaggcgtcg gcgaaattgc
     8821 cgaatcccgc gccagccccg gcgccggccg cggctgccgg gtccactccc gcatgcccga
     8881 actgatcgta ggctgcccgc ttctgggaat ccgacaggac ctcgtacgcc tccttggctt
     8941 ccttgaagcg gtcctcggcc ttggggttgt ccgggttgcg gtccggatgc cacttcatgg
     9001 cgagcttgcg atacgacttc ttgatgtcct cttccgaggc atcccggttc acgcccagca
     9061 cttcgtagta gtcgcgcttc gccattttgg gtactcagtt ccttcgcacg cgaaacggcc
     9121 gagttgaacg gcgaccccgt cgtccgttcg aactcggcca gccagaccgg cgcgtcggcg
     9181 tccggatccg gcgctatttc ttgtccttga cctcggtgta ctccgcgtcc accacgttgc
     9241 cgtcatcctt cttggccccg tcgtcggcct tggcctcgcc gcctgcctgc tgggcctgct
     9301 gtttagcctg ctcttccttg tacagggtct cggcgagctt gtgcgcggcc tgcgccagcg
     9361 cctgcgtctt ggcttcgatg gcggccttgt cgtcgctttt cagcacgtcc tccgcttcct
     9421 tcaatgcggc ctcgatcttg gccttttcgt cggcgtccac cttgtcgccg tagtccttca
     9481 gggacttttt caccgaatgc accatgtgat cgcactggtt gcgggcttcc accagctcgt
     9541 gcgccttgcg gtcctcctcg gcgttctcct cggcgtcctt caccatgcgc tggatttcct
     9601 gctcggtgag gcccgagctc gcctggatct tgatcttgtt ctccttgccg gttgccttgt
     9661 ccttggccga gacgtgcagg atgccgttgg cgtcgatgtc gaacatgacc tcgacctgcg
     9721 gcatgccgcg cggcgccggc ggaatgtcgg tcaggttgaa ctggccaagg ctcttgttgc
     9781 cgcgcgccat ctcgcgctcg ccctgaagca cgtggatcgt gaccgcggtc tggttgtcct
     9841 ccgcggtgga gaacacctgc tgcgccttgg tcgggatcgt ggtgttcttc tggatgagct
     9901 tggtcatcac gcccccgagc gtctcgatgc cgagggaaag cggcgtaacg tcgagcagca
     9961 acacgtcctt gacgtcgccc ttcagcacgc cggcctggac cgcggcgccg accgccacgg
    10021 cctcgtccgg attgacgtcc ttgcgcggct cgcggccgaa gaattccttt accttgtcct
    10081 gcaccttggg catgcgcgtc tgtccgccca ccaggatgac gtcgctgatg tcggcggcct
    10141 tgatgccggc gtccttgatc gccatccggc acggctcgat ggtgcgctcg atcaggtcct
    10201 cgaccaggct ctcgaacttg gcgcgcgtga ccttgaccgc caggtgtttc ggccccgact
    10261 ggtcggccgt aatgtacggc aggttgatct cggtctgcgc gcccgaggag agctcgatct
    10321 tcgctttctc ggcggcgtcc ttcagccgct gcagcgcgag gatgtccttc ttgaggtcga
    10381 tgccgctgtc cttcttgaac tcgtcgcaca cgtgctcgat caggcgctgg tcgaagtcct
    10441 cgccgccgag gaaggtgtcg ccgttggtgg agagcacctc gaactggtgc tcgccctcga
    10501 cgttggcgat ctcgatgatc gagatgtcga aggtgccgcc gcccaggtcg tagaccgcga
    10561 tcttgcggtc cttcttttcc tgtttgtcca tgccgaacgc gagcgcggcc gcggttggct
    10621 cgttgatgat gcgcttgacg acgaggcccg cgatgcggcc cgcgtccttg gttgcctggc
    10681 gctgcgagtc gttgaaatac gcgggcaccg tgatcaccgc ttcggtgacc ggctcgccga
    10741 ggtagtcctc ggcggtcttc ttcatctt
//
LOCUS       DBNGSG010000483         2111 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_836301, whole genome shotgun sequence.
ACCESSION   DBNGSG010000483 DBNGSG010000000
VERSION     DBNGSG010000483.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_836301"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="AC158Q_12800"
     CDS             complement(<1..1013)
                     /locus_tag="AC158Q_12800"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3340696.1"
                     /translation="MAARKRAGARKKTKTYDLHNHVIPENVVQAIRRDPERYGTRIEE
                     RGGKRFFDSHGRMTELLPEFCDVEAKVAWMDRVGMDVSVISVGPPIYFYWLAPGAGAE
                     IIRLANDGIAQMVAKHPDRLRGMAHLPMQDPDAAIAELERVVKDYKFKAVELATSIEG
                     LPLADAKFRKVLKAIEQLGLFIFTHPYQCLANGGMDAYYLRNFVGFPLDTTMMIAHLM
                     FSGALDDLKKLRFLVSHGGGFMPYQIGRFVHGHNVRPEPRVNNVSSPRELFKRFYFDC
                     LTHEPRSTRHLINMVGANRIVIGTDNPFDMAPKGDKLQVGQVDAIPGLTAEEREWICS
                     RTA"
     gene            complement(1083..2051)
                     /locus_tag="AC158Q_12805"
     CDS             complement(1083..2051)
                     /locus_tag="AC158Q_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3340697.1"
                     /translation="MKTLVSCVCFVAAVVAAGAVAAELSFPVRPIRIIVPQSPGASTD
                     ITARLVAQRLSEALNQPVIVDNRPGASGITGTELVARAARDGYTLMVVASSFSINPAL
                     GRKLPYDPIRDFATVAQLSKFPNMLAAHPSAPVKTLQDVIALARAKPGQLNYASAGLA
                     TGTHMTAELLKYMTGIDILHVPYSGGGPAMAAALGGQTQLVVSTTIGLLPHVRAGKLR
                     AIALTSAKRSAAAPEIPTFAESGVPGYEHEPWNGMFAPAGIPKPVLAKVNAEVARSLR
                     SPEVKKVFDRNGADVVGNTPEQFAAILKAEIAKWTKVAKAAGIKLE"
BASE COUNT          353 a          678 c          661 g          419 t
ORIGIN      
        1 ttcgcggtcc tgctgcatat ccactcccgc tcctctgcgg tcagccctgg tatcgcgtcc
       61 acctggccta cctgcagctt gtcgcctttc ggcgccatgt cgaaggggtt atcggtgccg
      121 atgacgatac ggttcgcgcc caccatgttg atgagatggc gggtggaacg cggctcgtgc
      181 gtgaggcaat cgaagtagaa gcgcttgaac agctcgcgcg gcgagctcac gttgttcacc
      241 ctgggctcgg ggcgcacgtt gtggccgtgc acgaagcggc cgatctggta cggcatgaaa
      301 ccgccgccgt gcgaaaccag gaaacgcagc ttcttcaggt cgtcgagcgc gccgctgaac
      361 atgaggtgcg cgatcatcat cgtggtatcg agcgggaagc caacgaagtt gcgcaggtag
      421 taggcgtcca tgcccccgtt cgcgaggcac tggtacggat gggtgaagat gaacaatccg
      481 agctgctcga tcgccttcag caccttgcgg aacttcgcgt ccgcgagcgg gagcccctcg
      541 atcgaggtgg ccagctcgac ggccttgaat ttgtaatcct tcaccactcg ttcgagctcg
      601 gcgatagccg cgtccgggtc ctgcatcggc aggtgcgcca ttccgcgcag gcggtccggg
      661 tgtttcgcca ccatctgcgc gatgccgtcg ttggcgaggc ggatgatctc ggccccggcc
      721 ccgggcgcga gccagtagaa atagatcggc gggccgaccg agatcacgga tacgtccatg
      781 ccgacgcggt ccatccatgc gaccttggcc tcgacgtcgc agaactcggg cagcagctcc
      841 gtcatgcgcc cgtggctgtc gaaaaagcgc ttgccgcccc tctcctcgat gcgcgtgccg
      901 tagcgttcgg ggtcgcgccg gatcgcctgc accacattct ccgggatgac gtgattgtgc
      961 aggtcgtagg tcttcgtttt ttttctcgcg cccgcgcgct ttcttgcagc catgcctgcc
     1021 ctttccaaca ataattgatc agaaaatacc gatgaaactt ccggccaccc cctcgtcact
     1081 cgtcactcga gcttgatgcc ggccgccttg gcgactttcg tccatttcgc gatctccgcc
     1141 ttgaggatgg cagcgaactg ctccggcgtg ttgcccacga cgtcggcgcc attgcgatcg
     1201 aatactttct tcacttccgg cgaacgcagg ctgcgggcga cctcggcgtt gactttcgcg
     1261 agcacgggct tcggtatacc ggccggcgcg aacatcccgt tccagggctc gtgctcgtag
     1321 cccggcacgc ccgactccgc aaaggtggga atttccggcg cggctgccga ccgtttggcg
     1381 ctggtcaggg cgatcgctct caacttgccg gccctcacgt gcggcagcag gccgatcgtg
     1441 gtgctgacga cgagttgcgt ctgcccgccc agtgccgccg ccatggccgg accgccaccg
     1501 ctgtagggca cgtgcaggat atcgattccg gtcatgtact tgagcaattc ggctgtcatg
     1561 tgcgtgccgg tggccaatcc ggccgatgca tagttgagct ggcccggttt cgccctcgcg
     1621 agtgcgatga cgtcctgcag cgtcttcacc ggcgccgacg ggtgtgcggc cagcatgttc
     1681 gggaacttcg agagttgcgc aacggttgca aaatcgcgga tcggatcgta cggcagcttg
     1741 cgccccaggg cggggtttat cgagaacgag gatgcaacta ccatcagggt atacccgtcc
     1801 cgcgcggccc ttgcgacgag ttcagtgccg gtgatgccgc tcgctccggg gcggttatcc
     1861 acgataaccg gctgattgag cgcctcgctc agcctctgcg cgaccagccg cgcggtgata
     1921 tcggtcgatg cgcccggcga ctggggcacg atgatccgga tcggccggac gggaaatgac
     1981 aactcggcgg ctaccgcacc cgcagcgacg accgccgcta caaaacatac gcaggacacc
     2041 agggttttca tgtctcctcc ttaagagggt gttgaaaaac gtgtcgcaca gaacgataac
     2101 gtcattcccg c
//
LOCUS       DBNGSG010000484         6596 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3383205, whole genome shotgun sequence.
ACCESSION   DBNGSG010000484 DBNGSG010000000
VERSION     DBNGSG010000484.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6596)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6596)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6596
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3383205"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="AC158Q_12810"
     CDS             <1..875
                     /locus_tag="AC158Q_12810"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000286 - L-alanine dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0042853 - L-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HIR3340698.1"
                     /translation="ARLRPGQAIFTYLHLAPDTEQARDLVGSGAIAIAYETVTAAGGG
                     LPLLAPMSEVAGRMAVQVAAHYLERPHGGRGVLISGATGVPPARILVLGSGVVGSNAA
                     RVGIGMGADVTVLGQSAGSLWRLSSDLGPSLRTVVSSPGAIERELSGADAVISAALVP
                     GALAPKLVTRAMLAGMKPGAVIVDVSIDQGGCFETSRPTTHSDPVYVVDGILHYCVAN
                     MPGAVPRTSACALNHATLPFVIELADRGIARALRENEHLRNGLNVYRGAVTRREVAEA
                     VGLPHVEALQALST"
     gene            994..2616
                     /gene="groL"
                     /locus_tag="AC158Q_12815"
     CDS             994..2616
                     /gene="groL"
                     /locus_tag="AC158Q_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185913.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIR3340699.1"
                     /translation="MTAKRLLFHNEAHAKMVAGMNILANAVRATLGPKARTVVLERPY
                     GAPAIINSGVVVAKEIELEDPFENMGAQMVREVASKTSDVAGDGTTTATLLAHGIVTE
                     GMKYVAAGMNPMDLKRGIDQAVVSVVSELKRLARPCASRTEIAQVATISANSDRVIGE
                     IVANAMEKVGKEGVITVEDGSGLESELEVVEGMRFDRGYLSPYFINSAEKHSVILEDV
                     YLLIHDRKIAAVKDLLPVLEQIAKAAKPLLVIAEEVEGEALATLVVNTLRGVLKTCAV
                     KAPGFGDRRKAMLDDIAVLTGGQVVAEEAGLTLERVDLAQLGRVKRVEIDKDNTTLIG
                     GAGDPAKIQARVAEIRQQIKDATSDYDREKLQERAAKLAGGVAVVKVGAATETEMKER
                     KARVEDALHATHAAVEEGVLPGGGVALLRARMLLGELRGDNPDQDAGIRIVLRALEEP
                     LRQLVANGGEEPSVVLNRVVEGKDGFGYNALTGQYGDMLQMGILDPCKVTRTALQNAA
                     SIAGLMLTTDCMIARIPEQPSAQPRAMGDMDM"
     gene            2840..3052
                     /locus_tag="AC158Q_12820"
     CDS             2840..3052
                     /locus_tag="AC158Q_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340700.1"
                     /translation="MKFPVDRHARVAGSRAARVAEAQMQAALRTRLLQAPDLFSDRDE
                     NAAEYASILQDFERLRNEATDADRFN"
     gene            3088..3543
                     /locus_tag="AC158Q_12825"
     CDS             3088..3543
                     /locus_tag="AC158Q_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3340701.1"
                     /translation="MTTIASICNRDVAFTTRETTIAAAAKLMRHGHVGCLVVVEQMNG
                     GRRTPAGIVTDRDIIVEVIAPGLDPATITVGDIMSSELVVGRENGSLQETTEIMRFKG
                     VRRLPIVGGDGQLVGIITIDDVLEVLADELNEIAKIVAREQSNESAARR"
     gene            3546..3917
                     /locus_tag="AC158Q_12830"
     CDS             3546..3917
                     /locus_tag="AC158Q_12830"
                     /inference="COORDINATES: protein motif:HMM:NF014534.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HIR3340702.1"
                     /translation="MAAQLQITVRDMPHSAALESRIRRQMAGLERIHPRITSFNVTLE
                     APHHHKHSGNHFNVKLDIRFPAGEIVVNRDHAEDIYVALRDAFQAARRQIIEHAERGQ
                     RADKTHRLRVRGAAPEGGLDE"
     gene            3910..4404
                     /locus_tag="AC158Q_12835"
     CDS             3910..4404
                     /locus_tag="AC158Q_12835"
                     /inference="COORDINATES: protein motif:HMM:NF037928.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HIR3340703.1"
                     /translation="MNRTCIIVADGGVSRFLGVEELESPRVRLALVERTVLKNNTDLK
                     SRGESVTGKVRTETNSDRGSGPVHPMGAQRERHRGELDRRFAMEITRQAADLTKGWEQ
                     GTVVLVAEPQLLGLMREALRKALHRGIELKELAKDYTHLTPPELLEHLALNKLIPAHR
                     RAAQ"
     gene            4512..6038
                     /locus_tag="AC158Q_12840"
     CDS             4512..6038
                     /locus_tag="AC158Q_12840"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HIR3340704.1"
                     /translation="MTLRKRPQPARAAAAPGKRDFPLDGIVASAMDAIITVDENQRIV
                     LFNKAAEKIFRCPAALAMGGPLDRFIPERFRGTHHGHIERFGRTGTTTRVMGERLHLY
                     GLRADGEEFPIDASISQVTAEGGKYYTVILRDVTERKAGEVALQRSYQELRAASERLL
                     GIIQSAMDAVVTIDHEQRIVMFNEAAEKIFRCPAGQAIGKPLDRFIPERFRAAHRGHV
                     EHFGATRVTTRMMGAQLELFGLRANGEEFPIDASISQVTVEGHKLYTVILRDVTERRR
                     AQEAQERSHRELRELSSTMHEVREAERLRIARELHDELAQWLTALKMDVSWLSSRLPR
                     EQAPLLEKTEKMKMLVDTTVAAVRRIAADLRPVMLDDLGLVAAMEHLLHDLSERTGIL
                     VSLDVGSGEFDFGEPLTTSLYRMAQEALTNVVRHAEATEARVELHLDRDSLVLAVRDN
                     GKGFDPEIAEGKKSYGVMGIRERAHTLGGRARIARLEEGGMLVEIVVPMERYRKGSAA
                     "
     gene            6035..>6596
                     /locus_tag="AC158Q_12845"
     CDS             6035..>6596
                     /locus_tag="AC158Q_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3340705.1"
                     /translation="MIRVLLADDHAVVRAGLREILADTGDIRVAGEATNGQEALARVR
                     EQEFDVAVLDMSMPGRNGIELIKLVKAEKPGIRILVLSMHSEAQYAVRAIRAGASGYL
                     GKESAADELVAAIRRIAGGGAYVSPETAERLALDAGRASNAPPHTLLSDREYQVFQLI
                     ANGATVGEIARQLSLSVKTVSTHKTRI"
BASE COUNT         1204 a         2077 c         2319 g          996 t
ORIGIN      
        1 gggcgcgcct gcgtccggga caggcgatct tcacctacct ccacctggcg ccggacacgg
       61 agcaggcccg cgatctggtc ggaagcggcg cgatcgccat tgcgtacgag accgtcaccg
      121 ccgccggtgg cggcctgccg ctgctcgcgc cgatgtcgga agtggcgggc cgcatggcgg
      181 tccaggtcgc cgcgcactat ctggagcggc cgcacggggg ccgcggcgtg ctgatatccg
      241 gcgcgactgg cgtgccgccg gcgcggatcc tggtgctggg ctccggcgtc gtcggttcca
      301 atgccgcccg ggtcggcatc ggcatgggtg ccgatgtgac cgtgttgggg cagtcggccg
      361 gctcactgtg gcgcctttcc tccgatctcg gcccgagcct gcggaccgtg gtctcgtcac
      421 ccggagcgat cgaacgggaa ctgagcgggg cggatgccgt catcagcgca gccctggtgc
      481 ccggcgcgct cgcgccgaaa ctggtcacgc gtgcgatgct ggccgggatg aaaccgggcg
      541 cggtgatcgt ggacgtctcg atcgaccagg gcggatgctt cgagacctcg cgcccgacca
      601 cccactcgga cccggtgtac gtggtggacg gcatcctgca ttactgcgtc gccaacatgc
      661 cgggcgcggt accgcgcacc tcggcctgcg cgctcaacca cgccacgctt ccgttcgtga
      721 tcgagcttgc cgatcgcggc attgcgcggg ccctgcgcga gaacgagcat ctgcgcaacg
      781 ggctgaacgt ctaccgtggc gcggtgacgc gccgcgaggt cgccgaggcc gtgggcttgc
      841 cccacgtcga ggcgcttcag gccctgtcca cctgacccgc cccggcgagc ggcccggtgc
      901 gcccgtgccc ggcgggcttg acctacatca aacccggggg cccggcaata cgggatcatc
      961 ttcacgccgg gcaatagacc gcggcgagga tatatgacag ccaagagact cctgtttcac
     1021 aacgaggcgc acgcgaagat ggtggcgggg atgaacatcc tcgccaatgc ggtacgcgcc
     1081 acgttggggc ccaaggcccg cacggtcgtg ctggagcgcc cctacggcgc gcccgccatc
     1141 atcaactcgg gcgtcgtggt cgcgaaggaa atcgagctgg aagacccgtt cgagaatatg
     1201 ggcgcgcaga tggtcaggga agtggcgagc aagacgtccg acgtcgcggg cgacggtacg
     1261 accacggcga cgctgctggc ccacggcatc gtcaccgagg gaatgaagta cgtggccgcc
     1321 ggcatgaacc cgatggatct caagcgcggg atcgaccagg cggtcgtgtc cgtggtgtcg
     1381 gaactgaagc gcctggccag gccctgcgcg agccgcaccg agatcgcgca ggtcgcgacg
     1441 atctctgcca actccgaccg cgtcatcggc gagatcgtcg ccaacgcaat ggaaaaagtc
     1501 ggcaaggagg gcgtcatcac tgtcgaggac ggctccgggc tggagagtga actcgaggtg
     1561 gtcgaaggta tgcggttcga ccgcggctac ctgtccccgt atttcatcaa ctcggccgag
     1621 aagcacagcg tgattctcga ggacgtctat ctcctcatcc acgaccggaa gatcgccgcg
     1681 gtcaaggatc tgctgccggt tctggagcag atcgccaagg ccgcaaaacc gctgctggtc
     1741 atcgccgagg aagtcgaggg cgaggcgctg gcgacgctgg tggtcaacac gctgcgcggc
     1801 gtgctgaaga cctgcgccgt caaggcgccc gggttcggcg accggcgcaa ggccatgctg
     1861 gacgacatcg cggtgctgac cggggggcag gtcgtggccg aagaggccgg cctgacgctg
     1921 gagcgcgtgg atctagcgca gctgggacgc gtgaaacgcg tggagatcga caaggacaac
     1981 acgaccctca tcggcggcgc gggcgacccg gcgaagatcc aggcgcgcgt cgccgaaatc
     2041 cggcagcaga tcaaggatgc gaccagcgat tacgatcgcg agaaattgca ggagcgggcg
     2101 gccaagctcg ccggcggtgt cgccgtggtg aaggtcggcg ccgcgaccga gacggagatg
     2161 aaggaacgaa aggcccgggt cgaggatgcc ctgcacgcca cgcatgccgc ggtcgaggag
     2221 ggtgtattgc cgggcggcgg cgtggcgctg ctgcgcgcgc gcatgctgct cggagaattg
     2281 cgcggggaca atcccgacca ggacgccggc atccgcatcg tcctgcgcgc gctcgaggag
     2341 ccgctgcgcc aactcgttgc gaatggcggc gaggagcctt cggtggtgct gaaccgcgtc
     2401 gtcgagggga aggacggctt cggttacaac gcgctgaccg gccagtacgg ggacatgctc
     2461 cagatgggca tcctcgatcc ctgcaaggtg acgcggacgg cgctgcagaa cgccgcgtcc
     2521 atcgccggtc tcatgctgac gaccgattgc atgatcgcga ggattcccga gcagccttcg
     2581 gcgcagccgc gcgccatggg cgacatggac atgtaacgcc ggtcgcgaca ggcaacaccc
     2641 cagccagcgc caggagcgct ggaagggctg cgcaaagtcg tcgtgcggca gcgcatggcg
     2701 gcaggttaac ggtgaggcac cgggccaggg aggccatgga attccggcgc ttctcgatac
     2761 tcccagcttg atccagatca agcgacttct gccggcgctt cgtatcatcg aagcgtgatt
     2821 gtcggagagg aggcgcgcga tgaagtttcc agtagatcgg catgcacggg tcgccggcag
     2881 cagggccgcg cgagtcgccg aggcgcagat gcaggcagcg ctgcggacgc ggttgctgca
     2941 ggcgccggat ctgttctccg accgtgacga gaatgcagcg gaatacgcgt ccatactgca
     3001 ggatttcgag cggctgagaa acgaggctac agacgcagac cgcttcaatt gaacggtatt
     3061 gggacactgg aacggaagga ggtcgagatg accactattg cttctatctg caaccgggac
     3121 gtcgcgttca cgacacggga gaccacaatt gccgcggcgg cgaagctgat gcggcacggt
     3181 cacgtcggct gcctggtcgt ggtggagcag atgaacggcg gcaggcggac gcccgccggc
     3241 atcgtgaccg accgcgacat catcgtcgag gtcatcgccc ccgggctcga tccggcgacg
     3301 attacggtcg gcgacatcat gtcatcggag ctggtcgtcg ggcgcgagaa cgggagcctg
     3361 caggagacca ccgagatcat gcgcttcaag ggcgtgagac gcctgccgat cgtcggaggc
     3421 gacgggcagc tcgtcggcat catcacgatt gacgacgtgc tggaagtgct cgccgacgag
     3481 ttgaacgaga tcgccaagat cgtggcgcgc gagcagtcga acgagtcggc ggcgaggcga
     3541 tgaccatggc ggcccagctg cagattaccg tgagggacat gccgcattcg gcggcgctgg
     3601 agtcgcgcat ccgcaggcag atggccgggc tggagcgcat ccacccgagg attacctcgt
     3661 tcaatgtcac gctggaggcc cctcatcacc acaagcacag cggcaatcac ttcaacgtga
     3721 agctcgatat ccggtttccg gccggggaaa tcgtggtgaa tcgcgaccat gccgaggaca
     3781 tctacgttgc cttgcgcgat gcatttcagg cggcccgccg gcagataatc gagcacgccg
     3841 aacgcgggca gcgcgcggac aagacgcacc gcctgcgcgt gcggggcgcc gctccggaag
     3901 gagggctgga tgaataggac ctgcatcatc gtggccgacg gtggggtttc ccgcttcctc
     3961 ggcgtcgagg aactcgaatc gccgcgcgtc aggctggcgc tcgtcgagcg cacggtgctg
     4021 aagaacaata cggacctcaa gtctcgcggc gagagcgtga ccgggaaggt gcgcacggag
     4081 accaactccg accgcgggtc cggcccggtt cacccgatgg gggcgcagcg ggagcggcac
     4141 cgtggcgagc tcgaccggcg attcgccatg gagatcaccc gccaggccgc ggatctcacc
     4201 aagggctggg aacagggtac cgtcgtgctg gtcgcggagc cgcagttgct gggcttgatg
     4261 cgcgaggcgc tacgcaaggc cctgcatcgg ggcatcgagt tgaaggagct cgcgaaggac
     4321 tacacgcatc tcactccgcc cgagctgctc gagcatcttg ccctgaacaa gctgattccg
     4381 gcgcatcgcc gcgccgcgca gtagctgccg ggcgccatct cccggcatcg ccgcaagtgc
     4441 ccctcggcgc cgccgatacc ggcggcgtca cgttcgttcc ggcgaaatgg agcgttcggt
     4501 atactggtgg cgtgacccta cgcaaacgac cgcagccggc tcgcgccgcc gcagcgccag
     4561 gcaagcgcga tttcccgctc gatggaatcg tcgcgtcggc gatggatgcc atcattaccg
     4621 tcgacgaaaa ccaaagaatc gtgctgttca acaaggcagc cgaaaaaata ttccgctgcc
     4681 cggcggcgct ggcgatgggc ggcccgctcg accgcttcat cccggagcgg ttccgcggga
     4741 cgcatcatgg gcacatcgag cgattcggca ggaccggcac gaccacccgg gtcatgggcg
     4801 agcggctgca cctgtacgga ttgcgcgccg acggcgaaga gttcccgatc gacgcctcga
     4861 tctcgcaggt taccgccgag ggcggcaagt actatacggt aatactgcgc gatgtcaccg
     4921 aacgcaaggc gggggaagtc gcgctgcagc gctcctacca ggaactgcgc gctgccagcg
     4981 agcgactgct cggcatcatc cagtcggcga tggatgccgt cgtcacgata gaccacgagc
     5041 agcgcatcgt gatgttcaac gaggccgccg aaaagatctt ccgctgcccg gccggacagg
     5101 ccatcggcaa gccgctcgat cgcttcattc ccgaacgctt tcgcgcggcg cataggggac
     5161 atgtcgagca tttcggggcg acgcgggtca ccacgcgcat gatgggagcg cagctagagc
     5221 tgttcggcct tcgcgcgaac ggagaagagt ttccgatcga tgcgtcgata tcccaggtta
     5281 ccgtcgaggg ccacaagctc tacacggtga tcctgcgcga cgtcacggaa cgccggcgcg
     5341 cccaggaggc gcaggaacgc tcccaccggg agctgcgcga gctgtcgagc acgatgcacg
     5401 aagtgcgcga ggccgagcgc ctgcgcatcg cgcgcgaact gcacgacgag ctggcgcagt
     5461 ggctcacggc gctcaagatg gacgtctcgt ggctttcctc gcggctgccg cgggagcagg
     5521 caccgctgtt ggagaagacc gagaagatga agatgctggt ggacaccacg gtcgccgcag
     5581 tgcggcgcat tgccgccgat ctgcgcccgg tcatgctgga tgacctgggc ctggttgccg
     5641 cgatggagca cctcctgcat gacctgtcgg agcgcacggg cattctcgtc agcctggacg
     5701 tcggatccgg tgagttcgat ttcggagagc cgctcaccac ctcgctctat cgcatggcgc
     5761 aggaggcgct gaccaacgtg gttcgtcatg ccgaggccac cgaagcgcgg gtcgaattgc
     5821 atctcgaccg cgacagcctc gtgctggccg tgcgtgacaa cggcaagggc ttcgacccgg
     5881 aaattgccga gggaaagaaa tcgtatggcg tcatgggaat ccgcgagcgc gcccatacac
     5941 tcggaggccg tgcccgcatc gcgcggctcg aggagggcgg aatgctggtc gagatcgtgg
     6001 ttcccatgga gcggtaccgt aaagggagcg cggcatgatt cgcgtgctgc tggccgacga
     6061 tcacgccgtg gtcagggccg ggttgaggga gatcctggcc gacaccggcg acatccgggt
     6121 ggcgggggag gctaccaacg ggcaggaggc gctcgcgagg gtgcgcgagc aggaattcga
     6181 cgtcgcggtt ctcgacatgt ccatgccggg caggaacggc attgagctga tcaagctggt
     6241 caaagccgag aagccgggta tccggatcct ggtgctcagc atgcacagcg aggcgcagta
     6301 cgcggtgcgt gcaatcaggg cgggcgcatc gggctatctc ggcaaggaga gcgcggctga
     6361 cgaactggtg gcggcaatac gccgtatcgc cggcggcggc gcctacgtca gtcccgagac
     6421 ggcggagcgc cttgcgctgg atgccgggcg tgcctccaac gccccgccgc acacgctgct
     6481 ctccgaccgg gaataccagg tattccaatt gatcgcgaac ggagcgacgg tgggcgagat
     6541 cgcaagacag ctctcgctct ccgtgaagac cgtgagcacc cacaagaccc gtatcc
//
LOCUS       DBNGSG010000485         3864 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_327259, whole genome shotgun sequence.
ACCESSION   DBNGSG010000485 DBNGSG010000000
VERSION     DBNGSG010000485.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_327259"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..127)
                     /locus_tag="AC158Q_12850"
     tRNA            complement(43..127)
                     /locus_tag="AC158Q_12850"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(91..93),aa:Leu,seq:gag)
     gene            complement(223..621)
                     /gene="secG"
                     /locus_tag="AC158Q_12855"
     CDS             complement(223..621)
                     /gene="secG"
                     /locus_tag="AC158Q_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007588165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HIR3340706.1"
                     /translation="METLVLVVHVIAGLAIIGLVLLQHGKGADVGAAFGSGSAGSVFG
                     SSGSANFLSRATAIAAVVFFLTSLGLTYISGSKTEHKGVMAAPPASQEKSLPAQIPPA
                     APAGQGAPAGKAAPAAPAADPGSKAQDVPK"
     gene            complement(622..1398)
                     /gene="tpiA"
                     /locus_tag="AC158Q_12860"
     CDS             complement(622..1398)
                     /gene="tpiA"
                     /locus_tag="AC158Q_12860"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HIR3340707.1"
                     /translation="MRVKLVAGNWKMNGNLASSQALLRAILPPLAELKRASYAACAPY
                     PYLAAVSQALRGTGVALGAQDLCQYGDGAYTGGVSGAMLADCGCRYVIVGHSERRGVF
                     GEPDNVVALKFAQAVKHELTPILCLGETLAEREAGTTEAVVARQLDAVIQHCGLQALG
                     RGVIAYEPVWAIGTGKTATPQQAQAVHAFIRGRISAADRRLGDLLQILYGGSVKAGNA
                     AQLFAMPDVDGGLIGGASLVADEFVAICRAAQVAEQTAKA"
     gene            complement(1650..2171)
                     /locus_tag="AC158Q_12865"
     CDS             complement(1650..2171)
                     /locus_tag="AC158Q_12865"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HIR3340708.1"
                     /translation="MAVPRKTYNVLFLCTGNSARSIMAEALLNGMGRGRFRAFSAGSH
                     PRGEVHSMAKELLRESCNSLAELRSKSWDEFVRPGSPRLDFVFTVCDNAAGEACPVWP
                     GRPVTAHWGIPDPAAVTGTEGEKRKAFFTAYNLLSCRISIFASLPLASLDRLMLKTKL
                     DEIGKIEPQQAQA"
     gene            complement(2185..2625)
                     /locus_tag="AC158Q_12870"
     CDS             complement(2185..2625)
                     /locus_tag="AC158Q_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009693515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HIR3340709.1"
                     /translation="MKRFHVHMAVEDVEQNVRFYSTVFGAAPTVSKPDYAKWMLEDPR
                     VNFAISKRGAKPGLDHVGIQAEDEAELEDVHARLARADQKLLRQEQTSCCYARSNKYW
                     VQDPQGVAWEAFHTLGEVPVFGDGTLVAQSEELGIKAACCAPVK"
     gene            complement(2622..2963)
                     /locus_tag="AC158Q_12875"
     CDS             complement(2622..2963)
                     /locus_tag="AC158Q_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR3340710.1"
                     /translation="METMIVVKAFGALAQETRLDIYRLLVAIGPEGLAAGAIAGKLEL
                     PPATLSFHLKELSLAGLVRSRQEGRFVIYAANFDTMIALVSFLTDNCCGGRACVPACQ
                     PAPIPKRRKAA"
     gene            complement(3029..3703)
                     /locus_tag="AC158Q_12880"
     CDS             complement(3029..3703)
                     /locus_tag="AC158Q_12880"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3340711.1"
                     /translation="MTRATTIIFDVDGTLIDSAADIHGALNYGLALAGCGSIEFGASR
                     ELIGLGLEGSLDKILADRGRRFEAGELARIRTACTAYYDAHLVVHTRLFADVRETLEV
                     FRRAGVAMGLCTNKRAEPTRHILAALGVSDYFGVLMARGSLPQGKPHPAPLLAAVDAL
                     GGRPEEAAMVGDSHIDIDCARAAGVASVAVSYGYSDRPVAELGADHVIERFCELPAVL
                     GAARNI"
BASE COUNT          689 a         1315 c         1155 g          705 t
ORIGIN      
        1 atgtcgaact cggtctctgt tcccctggtt ccttaacaac tatggtgccg acggagagat
       61 tcgaactctc acggcttgcg ccgccacccc ctcaagatgg tgtgtctacc aattccacca
      121 cgtcggccac taacttcggt gaatcgtgat tggtgaatgg tgattggaca agccgtgcgc
      181 agttcgtcca ttcactattc actattcact attcacgccc ttctacttcg gcacgtcctg
      241 cgccttcgag cccgggtccg cggcgggcgc ggcaggcgca gccttgccgg ccggcgcgcc
      301 ctgtcccgcg ggcgcagcgg gcgggatctg cgccggcagg gatttctcct ggctcgccgg
      361 cggcgccgcc atcacgccct tgtgctcggt cttgctgccc gaaatatagg tcaacccgag
      421 actcgtcagg aaaaaaacca ccgccgcgat cgccgttgcg cggctcagga agttggccga
      481 tcccgacgac ccgaacacgc tgccggcaga accgctgccg aatgctgcgc ctacgtcggc
      541 gcctttgcca tgctgcagca gcaccaggcc gatgatggcc aggcccgcga ttacatgtac
      601 caccagaacc agagtttcca tttatgcctt cgccgtttgt tcggcgacct gcgcggcacg
      661 gcagatcgcc acgaattcgt cggccaccag ggaagcgccc ccgatcagcc cgccatccac
      721 gtccggcatg gcaaacagct gtgccgcgtt gcccgccttc acacttcccc cgtaaagaat
      781 ctgcaggagg tcccccagcc ggcgatcggc ggccgagatt cggccgcgga tgaaggcatg
      841 caccgcctgt gcctgttgcg gagtcgccgt cttcccggtg ccgatcgccc agaccggctc
      901 gtaagcgatg accccccgcc cgagcgcctg cagtccgcaa tgctgaatca ccgcgtcgag
      961 ctggcgcgcc accaccgcct cggtcgtccc cgcctctcgc tccgccagtg tctcgcccag
     1021 gcacaggatc ggggtaagct catgcttgac tgcctgcgcg aacttgagtg caacgacatt
     1081 atcgggctcg ccgaatacgc cgcgtcgctc cgaatgaccg acgataacgt agcggcagcc
     1141 gcagtccgcc agcatcgcgc ccgaaacacc gccggtatac gcgccgtcac cgtattggca
     1201 caggtcttgc gcgccgagtg ccacgcccgt cccgcgcaat gcctggctga ccgccgcgag
     1261 atacggatac ggcgcgcaag ccgcgtagct ggcccgtttc agttccgcaa gcggcggcag
     1321 gatggccctg agcagcgcct ggctggaggc caggttgccg ttcatcttcc agttgcccgc
     1381 taccagcttg acgcgcattt cctgtaaggc tcacgtaaac cgttgaattt tacaggcgat
     1441 tggtcagcag ggtcaattaa gccgaggcct gttgcggcgc cgcagcacat tgaatccaaa
     1501 agaaaacgcc gggcgccagc cagcgatttt caattctcgg aattcggtca gggcgcagcc
     1561 gggtcaaccg aatcggtgcg ctgaaatgaa tgcgggcaat tcatcggaca tgcgtgccgc
     1621 caacgtgcgc cagcggtcta cgtccgcacc tatgcttgag cttgttgcgg ctcgattttg
     1681 cctatctcgt cgagcttcgt cttgagcata agccggtcga gactggcgag cggcaggctg
     1741 gcgaatatcg agatccggca ggagagcagg ttgtacgccg tgaagaacgc cttgcgcttc
     1801 tccccctcgg tccccgtgac tgcggcgggg tccgggatgc cccagtgggc ggtcacgggc
     1861 cgccccggcc acaccgggca ggcttcgccg gccgcgttat cgcaaaccgt gaacacgaag
     1921 tccagccgag gtgaacccgg cctgacgaac tcgtcccacg atttgctgcg cagctctgcc
     1981 aacgaattgc agctttcccg cagcagctcc tttgccatcg aatgcacttc gcctctaggg
     2041 tggctgcccg cgctgaacgc cctgaaccgt ccgcggccca tgccattcaa cagggcttcg
     2101 gccatgatgc tgcgcgctga attaccggtg cacaggaaca gcacgttata ggtttttcgc
     2161 ggaacagcca tgcccttgcc acgcttactt cacgggcgcg cagcaggcgg ccttgatacc
     2221 cagttcctcc gattgggcga cgagcgtgcc gtcgccgaat acgggaacct ctcccagcgt
     2281 gtgaaaggcc tcccatgcga cgccctgcgg gtcctgcacc cagtacttgt tcgatcttgc
     2341 gtagcagcag ctcgtctgct cctgcctcaa aagcttctgg tcggcccgcg caagccgggc
     2401 atgcacgtcc tccagttccg cctcgtcctc ggcctggatg ccgacgtggt cgaggcccgg
     2461 cttcgcgccg cgcttcgaga tcgcgaaatt gacccgcgga tcctcgagca tccatttcgc
     2521 gtaatccggc ttcgataccg tgggcgcggc gccgaacacg gtggaataaa acctgacgtt
     2581 ctgctcgacg tcctctactg ccatgtgcac gtgaaagcgt ttcatgcggc ttttctcctt
     2641 ttcgggattg gcgcgggctg acaggccggt acgcaggcgc ggccgccgca acagttgtcg
     2701 gtcaggaacg agaccagcgc gatcatggtg tcgaagttcg ccgcgtagat gacgaaccgc
     2761 ccctcttgcc gcgagcgcac caggccggcg agactcaatt ccttcagatg gaacgaaagc
     2821 gtcgcgggag gcaactcgag tttccctgca atcgcgcctg cagccagtcc ttccgggccg
     2881 atggcgacca gcaggcggta gatgtcgagg cgcgtctcct gcgccagcgc cccgaatgct
     2941 ttcacgacaa tcattgtttc catgattcca ataatatgaa aatattaaaa tggcgtcaag
     3001 gccaacaacc attcaatccg gcaaacagtc agatatttcg ggccgcgccc agtacggcgg
     3061 gcagttcgca aaagcgctcg atcacgtgat cggcgcccag ttccgcgacg gggcgatcgc
     3121 tgtagccata gctcactgca acggatgcga cgcccgccgc gcgcgcgcaa tcgatgtcga
     3181 tgtggctgtc accgaccatc gcggcttcct ccggccggcc gcccagggcg tcaacagcgg
     3241 ccagcagcgg cgcggggtgc ggcttgcctt gcggcagcga accgcgcgcc atcagaacgc
     3301 cgaagtagtc gctgacaccg agcgccgcca ggatgtgccg cgtgggttcc gctcgcttgt
     3361 tggtgcagag gcccatcgcc acaccggcgc gccggaacac ctcaagcgtt tccctcacgt
     3421 ctgcgaagag ccgcgtgtga acgaccagat gggcgtcgta gtacgccgtg cacgcggtac
     3481 gtatgcgcgc aagctccccc gcctcgaacc tgcggccgcg gtccgccagg atcttgtcga
     3541 ggctgccctc gagcccgagg ccgatcaact cgcgcgatgc gccgaactcg atgctgccac
     3601 aacccgccag cgcgaggccg tagttgagcg cgccatgtat gtccgccgcg ctgtcgatca
     3661 gcgtgccgtc gacgtcgaag atgatcgtgg ttgcgcgcgt caccggcagc gcccgtttcc
     3721 gtccgccatc aggtgatcgc ctggcggacc ctggcggaca gccactccga gatcaccacc
     3781 gtaatgaaga tcgcgatgaa aatgaccgtg acctggggcc acgcgagcgt ggataccgag
     3841 gcgttcagct gcagcccgat gccg
//
LOCUS       DBNGSG010000486         3372 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1176534, whole genome shotgun sequence.
ACCESSION   DBNGSG010000486 DBNGSG010000000
VERSION     DBNGSG010000486.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1176534"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="AC158Q_12885"
     CDS             <1..800
                     /locus_tag="AC158Q_12885"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HIR3340712.1"
                     /translation="WGQQVVIDNRSGAGGNIGMGIAAQAAGDGYTMLFVSSSLMVNPG
                     LYKKVPFDVYRSFIPISVLAGSTHVWFTHPSLPVKSIADLISLAKRDPTKSSVATPGI
                     GTVPHLSVHLLAYDSKANLVTVPYAGGGPSIAAVLGNQVPFGCQAIPPVTVHIQAGRV
                     RALAITSEKRSPIVPNVPTMAELGFKGHEAETITGLLVPAGTPPAIVKRLHGEVVRIM
                     ALPDIRQRVSDLGADISANSPQQFAAQIRREVNRWSKVIKDANIPAN"
     gene            1033..2199
                     /locus_tag="AC158Q_12890"
     CDS             1033..2199
                     /locus_tag="AC158Q_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008345702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HIR3340713.1"
                     /translation="MRIVDIREIAVPLESSLRNAAFDFSEMTTSVVAVITDVVREGRP
                     VAGFAFNSTGRYACGAQMRDRFIPRILKAPPESLVNATGDNLEPGKILACMMSREKPG
                     GHTERSVAIGTIEVAVWDAAAKIAGKPLHRLLSEKYSRGKLSKRVPCYVGGGWYEPGK
                     GLQELQDEIRRRLEQGYQTMKIKVGGATIPQDVGRIEAALKVVTSGDNLAVDANAAFD
                     RGRALSYAKALAPYELRWFEEPCDPLDYALLADVARDYGAPLATGENLFSTQDVHNLV
                     SYGGLRADRDILQMDVPQSYGIVQFSRTLEMLEGRGWKRKSVFPHGGNQMTLHIVGGF
                     GLGGCEAYPGVFGPFAGFADDARVENGTIDLPDRPGIGFEGQNALYAVMREMLN"
     gene            2287..2529
                     /locus_tag="AC158Q_12895"
     CDS             2287..2529
                     /locus_tag="AC158Q_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340714.1"
                     /translation="MPRIDPLPLTSDPETREALDNFRKSLGFVPNAALIMQHKPAIAR
                     GFALLSWAMWGPDSEVDVGFKRLLSHVSSSVHGCRY"
     gene            2530..2880
                     /locus_tag="AC158Q_12900"
     CDS             2530..2880
                     /locus_tag="AC158Q_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3340715.1"
                     /translation="MAHSAGASLRLGIPEDKFNAVWEYRASPLFTEAERVAIEFTVAA
                     SAQPNDVTDELFGKMKRHWTEGQIVEITGVIALFGFLNRWNDTMATPLEEEPARIGEK
                     HLAQRGWSAGKHGD"
     gene            2885..>3372
                     /locus_tag="AC158Q_12905"
     CDS             2885..>3372
                     /locus_tag="AC158Q_12905"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019847.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3340716.1"
                     /translation="MRIASALAALLRRLKDAVAALPYRGEGRFCPVCGKSSRRFRRFG
                     TVPRDDAQCVHCGALERHRFLWLYASRKTDLFDGAARRMLHVAPEPCLESRFKSRLGQ
                     GYLTADLSGRAMVRMDVANIEFADRSFDVIYCSHVLEHVPDDRRAMREFHRVLKEDGW
                     AI"
BASE COUNT          630 a         1031 c         1148 g          563 t
ORIGIN      
        1 cgtgggggca gcaggtggtg atagacaatc gcagcggcgc cggcggcaac atcggcatgg
       61 gcattgccgc gcaggcggcc ggcgacggct ataccatgct gttcgtgagt tcgagcctga
      121 tggtcaaccc cgggctctac aagaaagtcc cgttcgacgt ctaccggagc tttatcccga
      181 tctcggtgct cgcgggctcg acccacgtat ggtttaccca cccgagcctg ccggtgaaat
      241 ccatcgccga tctcatcagc ctggcgaagc gggacccgac caagtcgagc gtggcgacgc
      301 cgggcatcgg caccgttccc catctctcgg tgcatctgct cgcctacgat tccaaggcca
      361 acctcgtcac cgtgccgtac gcgggaggcg gaccgtccat cgcggcggtg ctgggcaacc
      421 aggtgccgtt cggctgccag gcgataccgc cggtaaccgt tcatatccag gcggggcggg
      481 tgcgcgcgct cgccattacc agcgagaagc gctcgccgat cgtgcccaac gtgccgacca
      541 tggccgaact gggcttcaag gggcatgagg cggaaaccat caccggcctg ctggtgccgg
      601 ccggcacgcc gcctgcgatc gtcaagcggc tgcacgggga ggtggtcagg atcatggcgc
      661 tgccggacat caggcagcgc gtgtccgacc tgggcgccga catcagcgcg aactctccgc
      721 agcagttcgc cgcgcagatc aggcgggaag tcaaccggtg gagcaaggtc atcaaggacg
      781 ccaatattcc cgccaactag aacagggatg agggcggagg gatgagggat gaggagaaga
      841 ctgccattag ccgtttttcc gatattcttt tgatggttcg tttcgttttt gtttctctcg
      901 gcgtcttggc ggttcagtag tgcgaatcac ggacatacgt gaaacagcgg tgccgctgaa
      961 gtcgagcctg cgcaagcccc accctctccc tcccctttgg cacgggggag ggtatgggga
     1021 gggggcgtag gcatgagaat tgtcgatatc cgggaaatag cggtaccgct tgaatccagt
     1081 ctgcgtaatg cagcgttcga cttcagcgag atgaccacct cggtggtcgc ggtcatcacc
     1141 gacgtggtgc gcgagggcag gccggtcgcg ggcttcgcgt tcaactccac cgggcgctac
     1201 gcctgcggcg cgcagatgcg cgaccgcttc atcccgcgca tcctgaaggc cccgccggag
     1261 tcgctcgtca atgcgacggg cgacaacctc gagcccggga agatactcgc ctgcatgatg
     1321 agccgcgaga agccgggcgg gcacaccgag cgttccgtcg ccatcggcac catcgaagtc
     1381 gcggtgtggg acgcggctgc caagatcgcc ggcaagccgc tgcatcgcct gctctcggaa
     1441 aaatacagcc gcggcaagct ttccaaaagg gtgccgtgct acgtcggcgg cggctggtac
     1501 gagccgggca agggcctgca ggagttgcag gacgagattc gccgcaggct cgagcagggc
     1561 taccagacca tgaagatcaa ggtcggcggc gcgaccattc cccaggacgt gggtcgcatc
     1621 gaggcggcgc tgaaggtggt cacgtcgggc gacaacctgg cggtggacgc caacgcggcc
     1681 ttcgaccggg gacgcgcgct gtcttacgcg aaggcactcg caccgtacga gttgcgctgg
     1741 ttcgaggagc cgtgcgatcc gctcgactac gcgctgctgg ccgacgtcgc gcgcgactac
     1801 ggcgcgccgc tcgccaccgg cgagaacctg ttctccacgc aggacgtgca caacctggtg
     1861 agctacggcg ggctgcgagc cgatcgcgac atcctgcaga tggacgtgcc gcagtcttac
     1921 ggcatcgtgc agttctcgcg cacgctggag atgctggagg gccgcggctg gaagcggaaa
     1981 tcggtattcc cgcacggcgg caaccagatg acgctgcaca tcgtcggcgg gttcggcctc
     2041 ggcgggtgcg aggcgtaccc cggggtgttc gggccgttcg cgggatttgc ggacgatgca
     2101 agggtcgaga acgggaccat cgatcttccc gaccggccgg ggatcgggtt cgaagggcag
     2161 aatgcgctgt atgcggtgat gcgtgagatg ctcaattagc cgccaagacg ccaaggcgcc
     2221 aagaagaaca gcaaatagac aggattaaca ggattcacaa gatcgaaaac ctagaccgga
     2281 gtccccatgc cgcgcatcga cccgttgccc ctgacgtcgg atcccgaaac ccgggaggcg
     2341 ctggacaatt tcaggaagtc gctcgggttc gtgccgaatg cggcgctcat catgcagcac
     2401 aagccggcga tcgcgcgcgg tttcgcgctg ctatcgtggg cgatgtgggg cccggacagc
     2461 gaggtcgacg tcggcttcaa gcggctgctg agccacgtat caagcagcgt ccacggttgc
     2521 cgctactgaa tggcgcacag cgccggggcg tcgctccggc tcggcatccc ggaggacaag
     2581 ttcaacgcgg tgtgggaata ccgcgcgagc ccgctgttca ccgaggccga gcgcgtggcg
     2641 atcgagttca cggtcgcggc atctgctcag cccaacgacg ttacggacga attgttcgga
     2701 aaaatgaagc gccactggac cgaaggccag atcgtggaga tcaccggcgt catcgcgctg
     2761 ttcggctttc tcaaccgctg gaacgacacg atggcaaccc cgctcgagga ggagccggcc
     2821 cgcatcggcg aaaagcacct cgcgcagcgc ggctggagcg caggaaagca cggggactga
     2881 ggtcgtgaga atcgcctcgg ccctggctgc cctgctcagg cgcctgaaag acgcggtagc
     2941 agcgctgccc taccgtggcg aggggagatt ctgcccggtt tgcggaaaat catcgcggcg
     3001 tttccgccgg ttcgggacgg ttccacgcga cgacgcgcaa tgcgttcatt gcggcgccct
     3061 cgagcgccac cgcttcctgt ggctttatgc atcgagaaag acggacctct tcgatggcgc
     3121 ggcaaggagg atgttgcacg tcgcgccgga accgtgcctg gaatcgaggt tcaaaagccg
     3181 cctcgggcaa ggttacctga ctgcggattt gtcagggcgc gcgatggtca ggatggacgt
     3241 cgccaacatc gagttcgcgg accggtcgtt cgacgtcatc tactgcagtc acgtgctgga
     3301 gcatgtgccg gacgacagga gggccatgag agagtttcac cgggtgctca aggaggacgg
     3361 ctgggcgata tt
//
LOCUS       DBNGSG010000487         5411 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1856095, whole genome shotgun sequence.
ACCESSION   DBNGSG010000487 DBNGSG010000000
VERSION     DBNGSG010000487.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5411)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5411)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5411
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1856095"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="AC158Q_12910"
     CDS             <1..476
                     /locus_tag="AC158Q_12910"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HIR3340717.1"
                     /translation="LNGIDATRQVKRELPEVRVVILSMHSSEEHVMQALRAGASGYLL
                     KESAPLELELALQAVGSGETYLSPPISRQVIEGYMQRVGDTTQDALATLSARQREILQ
                     LVAEGNSSKEIARRLDLSVKTIETHRAQLMDRLQIRDVAGLVRFAIRTGLISADK"
     gene            596..>5411
                     /locus_tag="AC158Q_12915"
     CDS             596..>5411
                     /locus_tag="AC158Q_12915"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR3340718.1"
                     /translation="MAGVKKRATTTKSKTGLRARRKTAGVKTPDTLRTVVVGGDAGYR
                     RAVLRLLKAHPRLAVAGSAGSAVEAEVLLQQCSPQLALIDVRLPDRSGFELVRDVKRK
                     HPDVKIVLIASEDGKEYRAAAALAGADGLMSRDELARQLSAWIRKFLAAPGSRRARGD
                     AQETLVTRSASTGAEKTRNRFRSTFNQAAVGIMHSTTEGWILEANRCLCDMLGYSSNE
                     LRTRNTRDLTHPDDRDRQDAQRLELLAGLRSSFSSEKRYVRKDGRVIWVNRTVALGYE
                     TPETDPYMIHTIEDITGRKHAVQALQQSQKQYAGLVDSLDSIVWEADAQTFQFTFVSQ
                     RAEKLLGYPVKRWLEEPTFWQDHIHPEDRDWAVAYCIAATHELRSHDFEYRMIAADGR
                     AVWLRDIVSVEAENGRPVRSRGVMVDITAGKQAEMDSRRHARARQVLGDCSHTLVHAN
                     DEAELLKEMCRIVVESGGYVQAHIGLALKDDRKTIAPVASAGYEAGYLESKQLTWSED
                     GSSASMTGRVVATGKQYISRDIFIDPHSRHLVERARRRGFRSMIGLPLKIEGTCIGAI
                     SIYAREQDAFDDEEVALLATLADDISFGMSSLRARAAHERTETELARVMRARTVLAEC
                     SRALVHAVNEADLLREMCRIIVDSGGYQQGWVGLANADPARPVLPVAHAGYQDDAPMA
                     YTGLSPDGRERGVAVTAMTSGETAVARDILNQPGHSWRRERALRNGFQSSIALPLRGD
                     GRVLGVLVMHAPEADAFSDEEIALLTTLVDDIGFGVIKLRADAARREAEARLVRLARA
                     RKVMAECSQALVHATDETRLLDGMCRIAVESGGFRQAWIAEVTGDNPLRVRAAAQAGY
                     GGGAPMTGASNWAADGSYQGLAAEAITSGAISIARDILNDPRQARKRHRALELNYQSS
                     IALPLREKGGVIGALVLHAAEKDAFDDEEIALLDGLVADLSYGIANLRMQRARLQAEA
                     AQRESEQRFRNTFEQAAVGITRVDLGGVLVDVNQKFCDMLGYSKDELLGRHVRDITHP
                     DDYGKGAQYRAELSHRAMSSAAGEKRFVRKDGSVLWARRTMSTACDETGKPLYLISVV
                     EDITERKELEQRYRETFDQAAVGIVHSALDGRYIRVNRKFCEMVGYADSELIGQTADL
                     LTHPDDLEQTRRNRRLMADGKADQQTGEKRYIRKDGRVIWVRRTASYARDAAGNPMYI
                     IRVIEDITERKHAESALQRKTELAQLLESLARAANEAATPESAMRTCLERLCEHGNWA
                     LGRLGIYGADEQERFPENTLWQSKDPARYEPVMQASVDPKYFSPRGIFITTVRKERRP
                     IWLADIASSPGFGRMRVALDCGLRSAFAFPVIVGDEVAAFLEFFGTEPREADTQLLEA
                     IGSVGAQLARLIERSRALKATLELAAIVESSNDAIIGRSLDGIITSWNAGAERMLGYS
                     AAEAIGQPIGFFLPPGQNVRISEINEKVQHGESITPHDTRRLTSDGRVLVVRSSISPL
                     RNSVGKVIGASQILQDVTALRRAEAVRSRLAAIVDSSSDAIISRGLNGEILTWNPAAE
                     RLFGWTAQEAIGRDMRLIVPPERIGENAPYREKVHEGLAVPDYDTWRLTKDGK"
BASE COUNT         1040 a         1682 c         1846 g          843 t
ORIGIN      
        1 gcctgaacgg gatcgatgcg acgcgccagg tcaagaggga gcttcccgaa gtcagggtcg
       61 tgatcctgtc catgcattcc tccgaggagc acgtgatgca ggcgctgcgt gcgggcgctt
      121 ccggatacct gctcaaggag tcggcaccgc tcgagctcga actcgcgctg caagcggtgg
      181 gcagcggcga gacctacctg agcccgccga tttcccggca ggtcatcgag ggctatatgc
      241 agcgagtcgg ggacactacc caggacgcgc tcgccaccct gtcggcacgg cagcgcgaga
      301 ttcttcaact ggttgccgag ggcaattcga gcaaggagat cgcgcgccgc ctcgatttga
      361 gcgtgaaaac gatcgagact caccgcgcac aactgatgga ccggctgcag atacgcgatg
      421 tggccggcct ggtgcgcttc gcgatacgga caggattgat ttctgcagac aagtaattgt
      481 tgtttatcag tatattaagc cgctttcatc aagttgaata ttagatctcc taggggcttt
      541 tacttcgcca actagggtat ctcccctata cagcgagcat cacagcccgc ctagcatggc
      601 aggcgtgaag aagcgcgcca ccacgaccaa gagcaagacg gggcttcgcg cccgccgcaa
      661 aacggccggc gtcaagacgc cggacaccct gcgcacggtt gtcgtgggcg gcgacgccgg
      721 ttaccggcgc gcggtgctgc gcttgctgaa ggcccacccg cgcctggcgg ttgccggcag
      781 cgcggggtcg gccgtcgagg ccgaggtctt gctgcagcaa tgcagtcccc agttggcgct
      841 gatcgacgtc aggctgcccg accgcagcgg attcgagctg gtccgcgacg tgaaacgaaa
      901 acatcccgac gtgaagatcg tgctgatcgc gagcgaggat ggcaaggaat atcgcgctgc
      961 ggctgcgctc gcgggcgccg acgggctgat gagccgggac gaactggcgc ggcaactttc
     1021 cgcatggatc aggaagttcc tggcagcacc cgggtcgcgg cgggcccgtg gagatgcgca
     1081 ggagaccctc gtgacccggt cagcgtccac cggcgcggag aaaaccagga accgcttccg
     1141 ctcgacgttc aaccaggcag ccgtcggcat catgcactcg accactgagg gctggatcct
     1201 ggaggcgaac cgctgcctgt gcgacatgct gggctattcc agcaacgagc tgcgcacgcg
     1261 caatacccgt gacctgacac acccggacga tcgcgaccgg caggatgccc agcgcctcga
     1321 gctgctggcc ggattgcgca gcagtttctc gagcgagaag cgttacgtgc gcaaggacgg
     1381 aagggtgatc tgggtaaacc ggacggttgc tctgggttac gagacaccgg aaaccgatcc
     1441 gtacatgatc cacacgatcg aggacatcac cgggcgcaag cacgccgtgc aggcgctgca
     1501 gcagtcgcag aaacagtacg cgggcctggt cgattcactg gacagcatcg tgtgggaggc
     1561 cgacgcgcaa acgttccagt tcaccttcgt cagccagcgc gccgagaaac tgctcggcta
     1621 tccggtcaag cgctggcttg aagagcccac gttctggcag gaccacatac acccggaaga
     1681 ccgggattgg gccgtcgcgt actgcatcgc agccactcat gaattgcgct cgcacgattt
     1741 cgagtaccgg atgatcgctg ccgacggccg cgcagtgtgg ctgcgcgata tcgtgagcgt
     1801 cgaggccgag aatggccgcc cggtgcgttc gcggggcgtg atggtggaca tcacggcagg
     1861 caagcaggcc gaaatggatt cccggcggca cgcccgggcg cgccaggtgc ttggcgactg
     1921 cagccacacc ctggtgcatg caaacgacga ggccgagctc ctgaaggaaa tgtgccgcat
     1981 cgtggtggaa tcgggcggtt acgtgcaggc gcatatcggg ctcgccttga aggacgatcg
     2041 caagacgatc gctccggttg cctcggccgg ctacgaggcc ggttatctcg aaagtaaaca
     2101 acttacctgg agcgaagacg gctcctccgc gagcatgaca ggtcgggttg tcgcgaccgg
     2161 caagcaatat atctcgcgcg acatattcat cgatccccat tcccgccacc tggtggagcg
     2221 agccaggcgc cgtggattcc gttccatgat cggcctgccg ctgaagatcg agggcacttg
     2281 catcggcgcc atcagcatct acgcgcgcga acaggacgcg ttcgacgacg aggaagtcgc
     2341 gttgctggcc acgctggcgg acgatatctc gttcggaatg tccagcttgc gcgcgcgcgc
     2401 cgcgcacgag cgcacggaaa ccgagctcgc gcgcgtgatg cgggcacgca cggtcctggc
     2461 ggagtgcagc cgcgcgctgg tgcacgcggt gaacgaggcg gacctgctcc gggagatgtg
     2521 ccgaatcatc gtggattccg gcggttacca gcagggttgg gttgggctcg cgaacgccga
     2581 tcccgcgcgc ccggttcttc ccgtggccca tgcaggctat caagacgatg cgccgatggc
     2641 ttacacggga ctgtcacccg acggacggga gcggggcgtc gccgtgacgg ccatgacgtc
     2701 cggcgagacc gccgttgcca gggacatatt gaaccagccc ggacattcat ggcggcgcga
     2761 gcgtgcgctg aggaacggct tccaatcctc gattgcgctg ccgttgaggg gcgacggacg
     2821 ggtgctgggc gtgctggtga tgcacgcgcc cgaagccgac gccttcagcg acgaggagat
     2881 cgcgctgttg accacgctgg tcgacgacat cggcttcggc gtgatcaagc tgcgggcgga
     2941 cgccgcccgc cgcgaagcgg aagcgcggct ggtccggttg gcgcgcgccc gcaaggtgat
     3001 ggccgagtgc agccaggccc tggtgcacgc aactgacgaa accaggctgc tcgacgggat
     3061 gtgcaggatc gcggtggaat ccggcggctt caggcaggcg tggattgccg aggtcacggg
     3121 cgacaacccc cttcgcgtgc gggccgcggc ccaggccggt tacgggggcg gcgcgccgat
     3181 gacgggcgcg agcaactggg cggcggacgg cagctaccag ggcctcgcag ccgaggccat
     3241 cacgagtgga gcgatcagta ttgcgcgcga catcctcaac gatccccggc aggcgcgcaa
     3301 gcgtcaccgc gcgctcgagc tgaactatca gtcgtcaatc gcgctgccgc tcagggagaa
     3361 gggcggcgtc atcggcgcgc tggtgctgca tgccgcggaa aaagacgcgt tcgacgacga
     3421 ggaaatcgcg ctgttggacg gattggtggc ggacctttcc tacgggattg ccaatctcag
     3481 aatgcagcgg gcgcgtttgc aggctgaggc ggcgcaacgg gagagcgagc agcggttccg
     3541 caataccttc gagcaggccg cggtcggcat cacccgcgtt gacctgggcg gcgtgctggt
     3601 ggacgtcaac cagaagttct gcgacatgct gggttacagc aaggacgaac tgctgggccg
     3661 gcacgtccgg gacatcacgc atcccgacga ctacggcaag ggggcgcaat accgggcaga
     3721 actgagtcac cgcgcgatga gttcggcagc gggggaaaag cgtttcgtcc ggaaggacgg
     3781 cagcgtgctg tgggcgcggc gcaccatgtc aaccgcctgc gatgagaccg gcaagccgct
     3841 ctacctgatc agcgtggtgg aggacatcac cgagcgcaag gaactcgagc agcgttatcg
     3901 cgagacattc gaccaggccg ccgtcggcat cgtgcacagc gcgctcgatg gccggtatat
     3961 ccgcgtcaac cggaaattct gcgagatggt cggctacgcg gattccgaat tgatcgggca
     4021 gaccgcggat ctcctcacgc atccggatga cctggaacag acccgccgta accggcgcct
     4081 gatggccgac ggcaaggcgg accagcagac aggcgagaaa cgctacatcc gcaaggacgg
     4141 ccgggtcatc tgggtcaggc gcaccgcctc gtacgcgcgc gacgcggccg gcaatccgat
     4201 gtacatcatc cgggtgatcg aagatatcac cgaacgcaag cacgccgaaa gcgccctgca
     4261 gcgcaagacc gagctggccc aattgctcga atccctggcg cgagccgcca acgaagcggc
     4321 cacgcccgaa agcgcgatgc gcacgtgcct ggaacggctc tgcgagcacg gcaactgggc
     4381 gctcggacgg ctcggcatat acggcgcgga cgagcaggag cgtttcccgg agaacacgct
     4441 gtggcagtcg aaggacccgg cacgctacga gcccgtcatg caggcttcgg tcgatccgaa
     4501 gtatttctcg ccccgcggca tattcatcac cactgtgcgg aaggagcggc ggccgatctg
     4561 gctggccgat atcgcatctt ccccgggttt cgggcggatg cgcgttgccc tggactgcgg
     4621 gctgcgctcg gcgttcgcct ttccggtgat cgtgggcgac gaggtcgcgg cctttctgga
     4681 attcttcggc accgagccgc gggaagcgga cacgcaactg ctcgaggcca tcggcagcgt
     4741 cggggcgcaa ctggcccgcc tgatcgagcg cagtcgcgca ttgaaggcga ccctggaact
     4801 ggcggcgatc gtcgaaagct ccaacgacgc catcattggc cgctcgctcg acggcatcat
     4861 cacctcatgg aatgccggcg cggagcggat gctgggctat tccgcagccg aggcgatcgg
     4921 ccagccgatc ggttttttct tgccgccggg acagaacgtc cgcatatcgg agatcaacga
     4981 aaaagtgcag cacggggaat cgatcacgcc tcacgacacc cgccggctga caagcgacgg
     5041 gcgagtcctc gtcgtgcgat ccagcatatc gccgctgcgc aacagcgtcg gaaaagtgat
     5101 cggcgcgtcc cagatccttc aggatgtgac ggccctgcgc cgcgccgagg cagtgcgctc
     5161 gcggctggcg gcgatcgtgg attcgagcag cgacgccatc atcagccgcg gattgaatgg
     5221 ggaaatcctc acctggaacc cggccgccga acggctgttc ggctggacgg cccaggaggc
     5281 gatcggacgg gacatgcgcc tgatcgttcc gcccgagcga atcggggaaa acgcgcctta
     5341 ccgggaaaag gtccacgaag ggctggcggt gccggattac gacacctggc gactcacgaa
     5401 ggacgggaag c
//
LOCUS       DBNGSG010000488         3741 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1008709, whole genome shotgun sequence.
ACCESSION   DBNGSG010000488 DBNGSG010000000
VERSION     DBNGSG010000488.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1008709"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..1265
                     /locus_tag="AC158Q_12920"
     CDS             147..1265
                     /locus_tag="AC158Q_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3340719.1"
                     /translation="MAKTAVVSEEMAQKFATEKETPYTRWVKTEGLDIIPSYYVPDLN
                     TVELKPWARRGGRGVYLNHEASRTTNDCYVCEIPAGGKLAPQRQLFEEMVMILSGRGS
                     TAVWNDAGGRITFEWKAGALFAIPVNAWHQHFNGSGETAARYVAVTNGPVVMNLYDDP
                     SFVFNTKYDFKSRFAGEPDYFAPKSEQKGFLLDTNFVADAVNLPLIAAKERGAGGGHI
                     RFNMAKGSMNSHISQFPTGTYKKAHAHGPGAHVIILSGEGYSLMWPEGEEPRRYEWNV
                     GAMIVPPNMWQHQHFNTGTTPARYLAFKHEGVAIRNAQGVPKAWISRRVGGDQIDYAD
                     EPPIVRRWFAEALAKHNLKSRMDEAYAAEVANLPPKAA"
     gene            1353..1910
                     /locus_tag="AC158Q_12925"
     CDS             1353..1910
                     /locus_tag="AC158Q_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3340720.1"
                     /translation="MGTESHYHSKKDRIFVRGIQGHYNLKDELKRLRSLPRVQKGSEL
                     KFVDGPQCYSRHYMEPVDGMTQTFHIHLEEYGPGGKSQKHGHVNEAAFYILDGAGYEI
                     HDGVRYDWQAGDIAIVHNNCVHQHHNADPDKPARALVLKTKPMYLFLNMLFQKTVEPR
                     PTEPTPGGKGFEAREGEQDYNHSPE"
     gene            1915..2523
                     /locus_tag="AC158Q_12930"
     CDS             1915..2523
                     /locus_tag="AC158Q_12930"
                     /inference="COORDINATES: protein motif:HMM:NF014136.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3340721.1"
                     /translation="MAWGESKAWLKGKTSGKLYQGMLDDVANAPARNAKRKKIVRPDE
                     MPWEMSRQGLLKHLLNEGMNTRMETVDAYMQIIPGGSRSGKHRHLAEECLYVLEGRGY
                     DLHQDCDVEITDTYHWIVQPEVKRYEWEAGDLIYIPPNTVHQHFNADPDRPVRLISAI
                     NRIYKFCGLNDLEQIEDAPEYDPKVVLTAEVLKRYLKVKETA"
     gene            2548..3132
                     /locus_tag="AC158Q_12935"
     CDS             2548..3132
                     /locus_tag="AC158Q_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HIR3340722.1"
                     /translation="MVRIVRWMSGLLGLAASGAAMAHVGNHAGSGLAAGLAHPFMGFD
                     HLLATAAVGVWAVQLGGRCLLLLPAAFIAVMAAGAAAGIYGITLPQVESMMALSVLAL
                     GMLVALAVKTPWHWPLSLIALFAMFHGHAHGSEMPELSTPWLYFAGFVTATACLHALG
                     VAAGAMLETHPRILRSGGVAVGLTGSWLLLAALA"
     gene            complement(3244..>3741)
                     /locus_tag="AC158Q_12940"
     CDS             complement(3244..>3741)
                     /locus_tag="AC158Q_12940"
                     /inference="COORDINATES: protein motif:HMM:NF036686.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maleate cis-trans isomerase family protein"
                     /protein_id="HIR3340723.1"
                     /translation="DPVDLVVYGCTAAGILAGPERDAGIAESLSGITGAPVVTTASSM
                     VESLREARARDIALVTPYAPEVNEQLKSLLARSGIAVRRMSSFGAGTVEELGRIGADA
                     VAARARETMDEECDALFIACSQLPTRSVVGPLEREFGRPVWSSIKATAWRAGETLGVS
                     LRYEG"
BASE COUNT          729 a         1232 c         1217 g          563 t
ORIGIN      
        1 atcatcacca cggcgatcgc aagggcggca agggatgagg aaaaaataca aacagagcgg
       61 gggggctccc tcatcccccg gtcagaggtg gccaaagcgc cgccgcagga gtagatttta
      121 atcagctgga accacgggag caggtaatgg ccaagaccgc tgtcgtttcg gaagagatgg
      181 cgcagaagtt cgccacggag aaggaaacgc cctacacccg ctgggtgaag accgagggcc
      241 tcgacatcat cccctcatat tacgtgcccg atctcaacac cgtcgagctg aagccctggg
      301 cccgccgcgg cggccgcggc gtgtacctca accacgaggc ctcgcgcacc acgaacgact
      361 gctacgtgtg cgagattccc gccgggggga agctcgcgcc gcagcgccag ctgttcgagg
      421 aaatggtcat gatcctgtcg gggcgcggct ccaccgcggt gtggaacgac gccggcggcc
      481 gcatcacctt cgaatggaag gccggcgccc tcttcgccat tcccgtcaac gcctggcacc
      541 agcatttcaa cggctccggg gagacggcgg cgcgctacgt cgcggtcacc aacggcccgg
      601 tggtgatgaa cctgtacgac gatccctcgt tcgtcttcaa cacgaaatac gacttcaaga
      661 gccgcttcgc cggcgaaccg gactatttcg cgcccaagag cgagcagaag ggcttcctgc
      721 tcgacaccaa tttcgtcgcg gacgccgtga acctgccgct catcgccgca aaggagcgcg
      781 gcgcgggcgg cggccatatc cgcttcaata tggccaaggg ctcgatgaac agccacatct
      841 cgcagttccc gaccggcacc tacaagaagg cgcacgccca cggcccgggc gcgcacgtga
      901 tcatcctctc cggcgagggc tactcgctga tgtggccgga gggcgaggag ccgcgccgct
      961 acgagtggaa cgtcggcgcg atgatcgtac cgcccaacat gtggcagcac cagcatttca
     1021 acaccggcac cacgcccgcg cgctacctcg cgttcaagca cgaaggcgtc gcaatccgca
     1081 acgcgcaagg cgtgcccaag gcctggatca gccggcgcgt gggcggagac cagatcgact
     1141 acgccgacga gcccccgatc gtccggcggt ggttcgccga ggcgctcgcg aagcacaatc
     1201 tcaagtcgcg gatggacgaa gcctacgccg ccgaggtcgc taaccttccg ccgaaggcgg
     1261 cgtagcgacc gcggcgaggc atgagggcgg aggggtgagg gatattgaag ttcgcggcgg
     1321 tataatgagc acgtctgatt agcgaggctc ccatgggcac cgaatcgcat taccacagca
     1381 agaaagaccg catattcgtg cgcggcatcc agggtcacta caacctcaag gacgagctga
     1441 agcggctgcg ctcgctgccg cgggtgcaga aaggcagcga actgaagttc gtggacgggc
     1501 cgcagtgcta cagccggcac tacatggagc cggtcgacgg catgacgcag acgtttcaca
     1561 tccatctcga ggaatacggc cccggaggca agtcgcagaa gcacggccac gtcaacgaag
     1621 cggcgttcta catcctcgac ggcgccggct acgagatcca cgacggcgtg cgctacgact
     1681 ggcaggccgg tgacatcgcg atcgtccaca acaactgcgt gcaccagcac cacaacgccg
     1741 atcccgacaa accggcgcgg gcgctggtgc tgaagaccaa accgatgtac ctgttcctca
     1801 acatgctctt ccagaagacg gtggagccgc gcccgacgga gcccacgccc ggcggcaagg
     1861 gattcgaggc gcgcgagggg gagcaggact acaaccattc accggagtga gcttatggcc
     1921 tggggtgaat cgaaagcgtg gttgaagggc aaaacgagcg gcaagcttta ccagggcatg
     1981 ctcgatgacg ttgcaaacgc gccggcgcgc aacgccaagc gcaagaagat cgtgcggccc
     2041 gacgagatgc cgtgggagat gtcgcggcag ggcctgctga agcacctgct gaacgaaggt
     2101 atgaacacgc gcatggagac ggtggacgcc tacatgcaga tcattcccgg aggcagccgc
     2161 tcggggaagc accgccatct cgccgaggag tgtctctacg tgctcgaggg ccgcggctac
     2221 gacctgcacc aggactgcga cgtcgagatc accgacacct atcactggat cgtgcagccc
     2281 gaggtcaagc gctacgagtg ggaagcgggc gatctcatct acattccgcc caacaccgtc
     2341 caccagcact tcaacgccga cccggaccga ccggtgcggc tgatctcggc gatcaaccgg
     2401 atctacaagt tctgcggcct gaacgatctc gagcagatcg aggacgcgcc cgagtacgat
     2461 cccaaggtcg tgctgacggc cgaagtgctg aagcgctacc tgaaagtcaa agagacggcg
     2521 tgacgagtga tgagaggaac tagaaacatg gtcaggatag ttcgctggat gtcgggtctc
     2581 cttggactcg ccgcgagcgg cgcggcgatg gcccacgtcg gaaaccacgc gggcagcggt
     2641 ttagccgccg gcctggcgca tccattcatg gggttcgacc acctgctcgc gacggcggcg
     2701 gtcggggtct gggcagtgca gctgggcggc cgttgcctgc tgctgctccc cgcggccttc
     2761 atcgcggtga tggcggccgg cgcggcggcg ggcatttacg gaatcaccct gccccaggtc
     2821 gaaagcatga tggcgttgtc ggtgctggcg ctcggcatgc tggtcgcgct ggccgtgaaa
     2881 accccatggc actggccgct atcgctgatc gcgctcttcg cgatgttcca tggacacgcc
     2941 cacggcagcg aaatgccgga attgtccacg ccgtggctgt acttcgcggg cttcgtgacc
     3001 gcgaccgcct gcctgcacgc gctcggcgtg gctgcgggcg ccatgctcga gacgcatccc
     3061 cgaattttgc gctccggcgg cgtggcggtc ggcctgaccg gcagctggct gctgctggcc
     3121 gcacttgcct gagcggcagt gacgagtgac cggtgacgag tgacgaatcg ccgaaaataa
     3181 aaaagggcgg cactgccgcc ctttccgctc ctgccgccgt cttggaccct catccctccg
     3241 ccctcatccc tcatacctga gcgaaacgcc cagcgtttcg ccggccctcc aggcggttgc
     3301 cttgatggat gaccagaccg gccgcccgaa ttcccgttcc agcgggccga ctaccgagcg
     3361 agtcggcagc tgcgagcagg cgatgaacag cgcgtcgcac tcctcgtcca tcgtctcgcg
     3421 cgcccgcgcg gcgaccgcgt ccgccccgat tcggcccagt tcctccacgg tgcccgcgcc
     3481 gaagctgctc atcctgcgca cggcgatgcc cgaccgcgcg agcagcgatt tcagctgctc
     3541 gttgacctcg ggcgcatagg gcgtcacgag ggcgatatca cgcgcgcggg cctcgcgcag
     3601 cgactcgacc atggagctcg ccgtggtcac gaccggcgct ccggtgatgc cggagagcga
     3661 ttcggcgatc ccggcgtcgc gctcggggcc ggcgagaatg ccggcggcgg tgcagccgta
     3721 gacgaccagg tccaccgggt c
//
LOCUS       DBNGSG010000489         9066 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2027094, whole genome shotgun sequence.
ACCESSION   DBNGSG010000489 DBNGSG010000000
VERSION     DBNGSG010000489.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9066)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9066)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9066
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2027094"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="AC158Q_12945"
     CDS             <1..344
                     /locus_tag="AC158Q_12945"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR3340724.1"
                     /translation="TLPRLLGHRKALELILLSDTFDARAALGLGLVNWVVGADQLEIE
                     TRSIARRLAQGATAAYGEIKRLVYAGQDRTFAAQLEAEVEAFARCAGTRDFAEGVTAF
                     VEKRKPNFEGK"
     gene            502..1323
                     /locus_tag="AC158Q_12950"
     CDS             502..1323
                     /locus_tag="AC158Q_12950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010110572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3340725.1"
                     /translation="MSGLKGKTVFITGATRGIGREIALRCARDGASIVVTGKSAEPHP
                     KLPGTIHSVAKEVEAGGGRALALALDVRDADAVAAAVKQTVKAFGGLDVLINNASAIS
                     LTGTLETPVKRLDLMLAVNLRGTFVCSQACIPHLRKADNPHILTLSPPLNMHPRWFKS
                     HVAYTMAKYGMSLCTLGMSEEFRAAGIAVNSLWPRTTIATAAIEVNFPEAILKASRKP
                     AIMADAAYAIVNRDSRKATGNFYIDETVLREEGVTDFDQYAVTPGTSLYTDLFLD"
     gene            1437..3242
                     /locus_tag="AC158Q_12955"
     CDS             1437..3242
                     /locus_tag="AC158Q_12955"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015645 - fatty acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HIR3340726.1"
                     /translation="MAQQPSHLISVEAAVTLDGLFRERVKRTPDLVAYRAYHEQHKNW
                     RDYTWGQIDHQVARWQAALEKEGLKAGDRVAVMLKNSPEWAIYDQAALGLGLVMVPLY
                     TQDRAENVAYIIGDAGCKVLLIEGAEQWQALSEVKHQLGSLNRILTVDSVPNPDEPRL
                     RWVGEWLPASGGETRHVPRDPHALATIVYTSGTTGRPKGVMLSHNNILSNAAAACTAV
                     LTTSHDDVLLSFLPLSHTFERTCGYYLTIMTGSTNAYSRGIPVLGEDLQSIRPTILIS
                     VPRIYERVYGRIRDALDAGSPLKKKLFLLAVEVGYARFEHAQGRGPWKPSFLLWPVLD
                     ALVAKKIRARLGGRLRAAISGGAALPPDISRVFIGLGLTVLQGFGMTECSPIAAANRP
                     DDNLPASVGTALPGVQLKVGVNSALMIKGPNVMLGYWNNPEATKAILGADGWLNSGDT
                     ARIDERGHVFITGRLKEIIVLSNGEKVPPVDIEAAIARDPLFEQVMLLGEGKPFLTVM
                     TVLGADPWKKLCAEKGIDPSPASASSKSTEEILRARIATQMKEFPGYAQVRRVTATLD
                     AWTVENGLLTPTMKLKRAKVMEKFNAEIDRMYAGH"
     gene            3272..3679
                     /locus_tag="AC158Q_12960"
     CDS             3272..3679
                     /locus_tag="AC158Q_12960"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR3340727.1"
                     /translation="MSEQRPTTTLPSGKEVTLRVVPMPANANQSGDIFGGWIMAQVDI
                     AGSIPAVRHAKGRVATVAVNSFQFKQPVLVGDVVSFYAEVVRVGRTSITVNVEVYAQR
                     KPEREQVVKVTEATLTYVAIDADRNPRVVSAQQ"
     gene            3793..5055
                     /locus_tag="AC158Q_12965"
     CDS             3793..5055
                     /locus_tag="AC158Q_12965"
                     /inference="COORDINATES: protein motif:HMM:NF015316.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HIR3340728.1"
                     /translation="MNACTKKVVAIAVGAALGGLAQGVVAGGFGIGTQSGSGTGNAFA
                     GGAAVADDASVAWSNPAGMSLLPRGKQVTGALHMLKPSFKFQNQGSTGVFAAPGTGDG
                     GDGGDWAFVPNGFFTMDINPQWRFGVALNVPFGLKTEYDSGWRGQLTGIKSEIKTVNI
                     NPSVAYQLNDKVSIGGGLSVQRIEAELTSFAGGAGISTLTADDAGYGFNLGLMAQMTP
                     ATRLGLSYRSRIKYDLEGTVAFSGAPAGNGNISADLKVPESFSLSVFHVVNPKWELMG
                     DITRTGWDSIQQLRVVRTSGPGAGTVLTTLVFNWKDTWRYGVGANYKWSEQTKLRFGL
                     ALDKTPTNDVDRTSRLPDQDRTWLAFGVQQRISKAGVLELAYAHEFIKDARVNNPVPG
                     FATCAAGCLTGSFENKADIFSVQYSHSF"
     gene            5172..7658
                     /locus_tag="AC158Q_12970"
     CDS             5172..7658
                     /locus_tag="AC158Q_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HIR3340729.1"
                     /translation="MPADSPFIVRKAAVLGAGVMGAQIAAHLANANVPAVLFELPAKE
                     GNPNGNVLKAIENLKKLEPSPLGTRSRADFIQAANYEQHLDLLKDCDLVIEAISERMD
                     WKADLYRKVAPHLAEHAIFASNTSGLSINKLAQAFPEGLRRRFCGVHFFNPPRYMHLV
                     ELIPAAGTDAAMLDDLEKFLVTALGKGVIRAKDTPNFIANRVGVFSMLATVHHAERLG
                     IGFDVVDALTGTLIGRPRSATFRTADVVGLDTFAHVVNTMKETLPQDPWHKYYVVPAW
                     LRQLIEQGALGQKTRKGVYQKIGNDIHVLDLKTQKYRLSDGKADEGVVEILKNKDVAA
                     RFEQLHASSHPQAQFLWAIHRDAFHYCAANLAEIADNARDLDLAIRWGFGWTQGPFEI
                     WQAAGWHTLARWISEDIAAGKGMTAAPLPAWASESSRKGVHGEEGSFAPAAKTYKPRS
                     SLPVYRRQPYPDRLLGEAVKYGETIFETDGVRCWHTGDDIAIVSFKSRMHAIGDDVLD
                     GVQQALEEAERNYMGLVVWQTEPPFSVGANLKKTPAGGARPSKPSALGRLFRSVKREA
                     ESLALKAARQIGVADQLMAGKLAEVEKLVEQFQDTTQALKFSMVPTVAAVDGLALGGG
                     CEFVMHCDRAVATLESYIGLVEAGVGLLPAGGGCKEFAMRAAADAKGGEMSPFLQKYF
                     KAVAMAEVGRSAEHSREIGYLRATDRVVMNRFELLEIAKAELRSLACAYRPPLRQVAI
                     PVGGRSAIATIRAFMENLLAGGHISEHDYLIGHKVAIVMCGGEIEGGSLVDEQWFLDL
                     ERKHFMELIATEKTQARIEHTLKTGKPLRN"
     gene            7762..8961
                     /locus_tag="AC158Q_12975"
     CDS             7762..8961
                     /locus_tag="AC158Q_12975"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011353151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HIR3340730.1"
                     /translation="MAKQIQDAYIVAAVRTPVGKAPRGMFRNVRPDDLLAYALKSALA
                     KCPGLDPAAIEDVIVGCAMPEAEQGMNVARIGLLLAGFPDTVSGMTINRFCSSGTQAV
                     ALAADRIRLGEADVMVAAGTESMSMVPMGGHKPSFGPAVFEKDENVAIAYGMGITAEK
                     VAAQWKVSREEQDLFAAESHRRAIRATDSGEFKDEITPYPIREHLPDLAAREVRINAR
                     DATNDEGPRRDTSPEALAKLRPAFSVKGSVTAGNSSQMSDGSAAVVLMSESALARYNL
                     RPLGRFMGYSVAGVPPDIMGIGPVKAIPKVLKQVGLSQEDIDWIELNEAFAAQSLAVI
                     KDLGLDRTRVNPLGGAIALGHPLGATGAIRVATLLHGLRRHKKKYGMVTMCIGTGMGA
                     AGVFEAL"
BASE COUNT         1727 a         2815 c         3035 g         1489 t
ORIGIN      
        1 ataccctgcc gcggctgctg ggtcaccgca aggcgctcga attgatattg ctgtcggaca
       61 ccttcgacgc gcgcgcggcg ctcggcctgg gcctggtgaa ctgggtcgtg ggcgcggatc
      121 agctcgagat cgagacgcgg tccatcgcgc gccggctggc gcagggcgcg acggcggcct
      181 acggcgagat caaacgcctg gtctatgcgg gacaggaccg gacgttcgcg gcgcagctcg
      241 aggcggaggt ggaagcgttc gcgcgctgcg cgggcacgcg cgacttcgcg gagggtgtga
      301 ctgctttcgt tgaaaagcgc aaacccaact tcgagggaaa atagccgcca agacgccaag
      361 gccgccaaga agatcaacaa tcaatacaaa taaagaacaa gaacgaagct tttcgacgga
      421 tttctcttat tgattttatt gttgtctgtt ctggtttcct tggcgtctcg gcgccttggc
      481 ggctaatttg aggtcttacg tatgtcgggg ttgaagggaa aaacggtttt catcaccggc
      541 gcgacgcggg gcatcgggcg cgagatcgcg cttcgctgcg cgcgcgacgg cgccagcatc
      601 gtcgttaccg gcaagtccgc cgaaccgcac ccgaagctgc caggcactat ccacagcgtg
      661 gcaaaggagg tcgaagccgg cggcggcagg gcgctcgcgc tggccctcga cgtgcgcgac
      721 gccgatgccg tcgcggccgc cgtgaagcag accgtcaagg cgttcggcgg cctcgacgtc
      781 ctcatcaata atgccagcgc catctcgctc accggcacgc tcgagacgcc ggtgaagcgc
      841 ctcgatctca tgctggcggt caacctgcgc ggcaccttcg tctgctcaca ggcctgcata
      901 ccccatctca ggaaagctga taatcctcat atacttacgc tatcgccgcc gctcaacatg
      961 cacccccgct ggttcaagtc gcacgtcgcc tataccatgg cgaaatacgg catgagcctg
     1021 tgcacgctcg gaatgtcgga ggaattccgg gcggccggca tcgcggtgaa ttcgctgtgg
     1081 ccgcgaacga caatcgcgac ggcggccatc gaggtgaact tccccgaggc gatcctcaag
     1141 gcgagccgca agcccgcgat catggccgac gccgcctatg cgatcgtgaa ccgggacagc
     1201 cgcaaggcga ccggcaactt ctacatcgac gagacggtac tgcgggagga gggcgtcacg
     1261 gatttcgatc aatacgccgt cacgcccgga acgagtttgt atacggacct gttcctcgat
     1321 tgacgagagc gtgattcgtg agtggggagt cgatttcccg gcagcgtgcc ggattttgat
     1381 caaatcacga atcacgattc acgaatcacc agcgataggg aaaaacggag gagaccatgg
     1441 cacaacaacc cagccacctg atttccgttg aggcagcagt aaccctggac ggcctgttcc
     1501 gcgagcgcgt gaagcgcacg ccggacctcg tcgcgtaccg cgcttaccac gagcagcaca
     1561 agaactggcg cgactacacc tgggggcaga tcgaccacca ggtcgcacgc tggcaggccg
     1621 cgctcgagaa ggagggtctg aaagccggcg accgcgtcgc cgtcatgctg aaaaactccc
     1681 ccgagtgggc gatttacgac caggcggcgc tcgggctcgg gctggtgatg gtgccgctct
     1741 acacccagga ccgcgcggag aacgtcgcct acatcatcgg cgacgccggc tgcaaggtgc
     1801 tgctgatcga gggcgccgag cagtggcagg cgctgtccga agtcaagcac cagctcggga
     1861 gcctgaatcg aatcctgacc gtcgactcgg tgcccaaccc ggacgagccg aggttgcggt
     1921 gggtcggaga gtggctgccg gcgagcggcg gcgagacacg ccacgtgccg cgcgacccgc
     1981 acgcgctcgc caccatcgtc tatacctcgg gcacgaccgg ccggccgaag ggcgtgatgc
     2041 tgtcgcacaa caacatcctg tcgaacgccg cggcggcttg cacggcggtg ctgacgacca
     2101 gccacgatga cgtgctcctg tcgtttctgc cgctgtcgca taccttcgag cgcacctgcg
     2161 gctattacct caccatcatg accggttcca cgaacgcgta ctcgcgcggc atcccggtgc
     2221 tcggcgaaga tttgcagtcg atccgtccga cgattctgat ttccgtgccg cgcatctacg
     2281 agcgcgtgta cggccgcatc cgcgacgcgc tcgacgcggg ctcgccgctg aagaagaagc
     2341 tgttcctgct ggcggtggag gtcggttatg cgcgtttcga gcacgcgcag ggccgcggcc
     2401 cgtggaaacc gtcgttcctg ctatggccgg tgctcgatgc gctggtggca aagaaaatac
     2461 gcgcgcgtct cggcgggcgc ctgcgggcgg ccatctcggg cggggccgcg ctgccgccgg
     2521 acatttcgcg cgtgttcatc gggctcgggc ttacggtgct gcagggcttc ggcatgaccg
     2581 aatgcagtcc gatcgcggct gccaaccggc cggacgacaa cctgccggcg agcgtcggca
     2641 cggcattgcc gggggtgcaa ttgaaggtcg gagtgaacag cgcgctcatg atcaagggtc
     2701 cgaacgtgat gctcggttac tggaacaacc cggaggcgac gaaagcgatc ctcggcgcgg
     2761 acggctggct caactcgggc gacaccgcgc gcatcgacga gagggggcac gttttcatca
     2821 ccggccggct gaaggagatc atcgtgctgt ccaacggcga gaaagtgccg ccggtggaca
     2881 tcgaggccgc gatcgcgcgc gatcccctgt tcgagcaggt gatgctgctg ggcgagggca
     2941 agcccttcct gacggtgatg acggtcctcg gcgccgaccc gtggaagaag ctgtgcgcgg
     3001 aaaaggggat cgatccctcg cctgcatcgg caagctcgaa gtccacggag gaaatcctgc
     3061 gcgcgcgtat cgcgacgcag atgaaggagt tcccgggcta tgcgcaggtg cgccgcgtga
     3121 cggcgacgct cgatgcgtgg acggtggaga acggactgct caccccgacc atgaaactga
     3181 agcgcgccaa ggtgatggag aagttcaatg ccgagattga caggatgtac gcggggcact
     3241 aattgcagtg acgagtgacg agtgacgagt gatgagtgaa caacggccga cgacgacgct
     3301 gccctccggc aaggaagtga cgctgcgcgt ggtgccgatg ccggccaacg cgaaccagag
     3361 cggggacatc ttcggcggct ggatcatggc gcaggtggat attgccggca gcatccccgc
     3421 cgtgcgtcac gccaagggcc gggtcgcgac agtggcggtg aactcgttcc agttcaagca
     3481 gccggtgctg gtgggggacg tcgtcagctt ttatgcggaa gtggtgcgcg tcgggcgcac
     3541 ctcgattacc gtcaacgtcg aggtctatgc gcagcgcaag cccgagcgcg aacaggtcgt
     3601 caaggtgacg gaggcgacgc tcacctacgt ggccatagat gcagaccgga atccacgtgt
     3661 tgtatcagcg caacagtagg ccgtttaccc tcggggaccc gtgttgtaaa aacgtctccc
     3721 gggagtaaga tggttcgtca catggcccgc ccgcatgacc cgggcgccgc catcaaaaaa
     3781 gggggagtca tcatgaacgc gtgcacgaag aaggtcgttg ctatcgctgt cggcgcggca
     3841 ttgggcgggt tggcgcaggg cgttgtcgcc ggcggtttcg ggatcggcac ccaaagcgga
     3901 agcggaaccg gtaatgcgtt cgcgggcggt gcagcggtcg cggatgacgc aagcgtcgcc
     3961 tggtccaatc cggcgggaat gtcgttgctg ccgcgcggca agcaggtcac cggcgcacta
     4021 cacatgctca agccctcgtt caagttccag aaccagggct cgaccggagt atttgcggcg
     4081 ccgggcaccg gcgacggcgg cgacggcggg gactgggcgt tcgttcccaa cggcttcttc
     4141 acgatggata tcaacccgca atggcgtttc ggggtggcgc tcaacgtgcc gttcgggctg
     4201 aagaccgaat acgactccgg ctggcgcggg cagttaacgg ggatcaagtc cgagatcaag
     4261 accgtcaata tcaacccgtc ggtggcgtac cagcttaacg acaaggtttc catcggcggc
     4321 ggcttgagcg tgcagaggat cgaagccgaa ttgacgagct tcgcgggcgg cgccgggatt
     4381 tctacgctga cggcggacga cgcaggctac ggcttcaacc tcgggttgat ggcgcagatg
     4441 acgcctgcta cccggctcgg cctgtcgtac cggtccagga tcaagtacga cctcgagggc
     4501 acagtggcct tcagcggcgc tccagcgggc aacggcaata tctcggcgga cctgaaagta
     4561 ccggagagct tttccctgag cgtgttccat gtcgtgaacc cgaaatggga actgatgggc
     4621 gacatcaccc ggaccggctg ggattccata cagcaattga gggtggtccg caccagcgga
     4681 ccgggggcag gcacggtttt gacaacgctc gtatttaact ggaaggatac atggcgctat
     4741 ggcgtgggag ccaattacaa gtggagcgaa cagaccaagc tccgcttcgg cctcgcgctc
     4801 gacaagacgc ccacgaacga cgtggaccgc acgtcgcgac tgccggacca ggaccgcacc
     4861 tggctcgcgt tcggcgtgca gcaacgcatc tcgaaggcgg gcgtcctcga gctggcgtac
     4921 gcgcacgagt tcatcaagga cgcgcgcgtc aacaatccgg ttcccggctt cgccacgtgc
     4981 gccgcgggat gcttgacggg cagcttcgag aacaaggccg acatcttctc ggttcagtat
     5041 tcgcattctt tctaggcgag cgcggaaaac ggagaaggcc gggcgctttt cgtccggctt
     5101 tttcatttcc cgtccgcata attcaccgca aaatcgcgtg ctagaatcag cgttcctttc
     5161 gcgggggtcc aatgcctgct gactctccct tcatcgtccg caaggctgct gtgctcggcg
     5221 ccggagtgat gggcgcgcag atcgccgcgc acctggccaa cgcgaacgtt cccgccgtgc
     5281 tcttcgagct tcccgccaag gagggcaatc ccaacggcaa cgtgctgaag gcgatcgaga
     5341 acctcaagaa gctcgagccc agcccgctcg gcacccggtc gagggcggac ttcatccagg
     5401 cggcgaacta cgaacagcat ctcgacctgc tgaaggactg cgacctcgtg atcgaggcga
     5461 tctccgagcg catggactgg aaggcggacc tgtaccgcaa ggtcgctccg cacctcgccg
     5521 agcacgcgat cttcgccagc aatacctcgg gcctttcgat caacaagctc gcgcaggcgt
     5581 tcccggaggg gctgcgccgg cgcttctgcg gcgtgcattt cttcaacccg ccgcgctaca
     5641 tgcatctcgt cgagctgatc cccgcggccg gcacggacgc tgcgatgctc gacgatctcg
     5701 agaagtttct cgtcaccgcc ctgggcaagg gggtgatccg agccaaggac acgcccaact
     5761 tcatcgccaa ccgcgtcggc gtgttctcga tgctggcgac ggtccatcac gctgagcggc
     5821 tcggcatcgg cttcgacgtg gtggatgcgc tgaccggcac gctgatcggg cgcccgcgca
     5881 gcgcgacctt ccgcaccgcc gacgtggtcg ggctcgatac cttcgcgcac gtcgtcaaca
     5941 ccatgaaaga gacgctgccg caggatccct ggcacaagta ttacgtcgtg ccggcgtggt
     6001 tgaggcaact gatcgagcag ggcgcgctcg ggcagaagac gcgcaagggc gtgtaccaga
     6061 agatcggcaa cgacattcac gtgctcgatc tcaagacgca aaaataccgg ctgtcggacg
     6121 ggaaggctga tgaaggcgtg gtcgaaatcc tcaagaacaa ggacgtcgcg gcgcgattcg
     6181 agcagctgca tgcctccagc catccgcagg cgcagttcct gtgggcaatt caccgcgacg
     6241 cattccatta ctgcgcggcc aatctcgcgg aaattgccga caacgcgcgc gacctcgatc
     6301 tcgccatccg ctggggtttc ggctggaccc agggaccttt cgagatctgg caggccgcgg
     6361 gttggcatac gctcgcccgg tggatttccg aagacatcgc ggcaggcaag ggcatgacgg
     6421 ccgccccgct gccggcgtgg gcgtcggaat catcgcgcaa gggcgttcac ggcgaggagg
     6481 gctcattcgc gccggcggcg aagacctaca agccgcgttc ctcgctgccg gtctaccgcc
     6541 gccagcctta tcccgaccgc ctgctgggcg aggcggtgaa atacggcgag accatattcg
     6601 agaccgacgg cgtgcgctgc tggcataccg gcgacgacat cgcaatcgtc agcttcaaga
     6661 gccgcatgca tgccatcggc gatgacgtgc tcgacggcgt gcagcaggcg ctggaggagg
     6721 ccgagcgcaa ctacatgggc ctcgtcgtct ggcagaccga gccgccattc tcggtcggcg
     6781 ccaacctcaa gaaaacgccc gccggtggcg cgaggccgtc gaaaccgtcg gcgctgggca
     6841 ggctgttccg gtcggtcaag cgcgaggccg agtcgctcgc gctcaaggcg gcacggcaaa
     6901 tcggcgtcgc cgaccagctg atggcgggaa agctcgccga ggtggaaaag ctagtcgaac
     6961 agttccagga cacgacgcag gcgctgaagt tttccatggt gccgaccgtg gccgcggtgg
     7021 acggccttgc gctgggcggc ggctgcgaat tcgtcatgca ctgcgaccgc gcggtggcta
     7081 cgctcgagag ctacatcggg ctggtcgagg ccggtgtcgg gctgctgccg gcgggcggcg
     7141 gttgcaagga gttcgcgatg cgcgccgcgg cagacgccaa gggcggcgag atgtcgccgt
     7201 ttctgcagaa gtatttcaag gcagtcgcga tggcggaagt ggggcgcagc gccgagcatt
     7261 cccgggagat cggctatttg cgggcgaccg accgcgtggt gatgaaccga ttcgagttgc
     7321 tcgaaattgc caaagccgag ctgcgctccc ttgcctgcgc ataccgcccg ccgctcaggc
     7381 aggtcgcgat tccggtcggg ggacgcagcg cgatcgcgac gatccgcgca ttcatggaga
     7441 atctcctcgc cggcgggcac atctccgagc acgactacct cattggccac aaagtcgcga
     7501 tcgtgatgtg cggaggcgag atcgagggcg gcagcctggt ggacgagcag tggttcctcg
     7561 acctcgagcg caagcacttc atggaattga tcgctacgga aaaaacgcag gccaggatcg
     7621 agcatacgct caagacggga aagcccttga gaaattagga ttcgggatcc aggattcagg
     7681 attcggcgaa aaccgaagac gctcgatgcc gttccctgac tcccgaatcc tgcctcctga
     7741 atccttgtta ggagaaacgc gatggccaaa caaatccagg atgcttacat agtcgccgcc
     7801 gtccggactc ccgtgggcaa ggcgccgcgc ggcatgttca ggaacgtgcg gcccgacgac
     7861 ctgctggcgt acgcgctgaa aagcgcgctc gcgaagtgtc cgggactcga tcccgcggcc
     7921 atcgaggacg tgatcgtcgg ctgtgccatg ccggaggcgg agcagggaat gaacgtggcg
     7981 cgcatcgggc tgctgctcgc gggctttccc gacaccgttt ccggcatgac gatcaaccgc
     8041 ttctgctcgt cgggaacgca ggcggtggcg cttgccgcgg accgcatccg cctgggcgag
     8101 gcggacgtga tggtcgccgc cggaaccgaa agcatgagca tggtgccgat gggcggccac
     8161 aagccgtcgt tcggtccggc ggtcttcgag aaggacgaga acgtcgcgat cgcttacggc
     8221 atgggcatta ccgccgagaa ggtggccgcg cagtggaagg tgagccgcga ggagcaggac
     8281 ctgttcgccg cggagagcca tcgccgcgcc atccgcgcca ccgacagcgg tgaattcaag
     8341 gacgagatca cgccgtaccc gatccgcgaa cacctgcccg acctggcggc gcgggaggtg
     8401 cggataaacg cccgcgacgc cacgaacgac gagggccccc ggcgggacac gtcgcccgag
     8461 gcgctcgcga agctgcggcc cgcgttttcg gtaaaaggca gcgtcaccgc cggcaacagc
     8521 tcgcaaatgt cggacgggtc ggcggcggtg gtgctgatga gcgagtccgc gctcgcgcgc
     8581 tacaacctgc gcccgctggg ccgcttcatg ggctattcgg tggcaggcgt gcccccggac
     8641 atcatgggta tcggaccggt caaggcgatc ccgaaggtgc tgaagcaggt ggggctcagc
     8701 caggaagaca tcgactggat cgaactgaac gaagcgtttg ccgcgcaatc gctcgcggtg
     8761 ataaaagacc tcggtctcga ccgcacccgg gtcaacccgc tcggcggcgc gatcgcactc
     8821 gggcacccgc tgggggcgac cggcgcgatc cgggtggcca cgctcctgca cggcttgcgc
     8881 cgtcacaaga agaaatacgg catggtcacc atgtgcatcg gcacggggat gggtgcggcg
     8941 ggcgtgttcg aggctttgtg agcccgctac accccgcacg acggctgagc cagcccgggt
     9001 cgactcacga ctcgcgaatc acgactcgcg caatttcagc tgcgaggagg aggagcatgt
     9061 aatcca
//
LOCUS       DBNGSG010000490         3845 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3217327, whole genome shotgun sequence.
ACCESSION   DBNGSG010000490 DBNGSG010000000
VERSION     DBNGSG010000490.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3217327"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1211
                     /locus_tag="AC158Q_12980"
     CDS             <1..1211
                     /locus_tag="AC158Q_12980"
                     /inference="COORDINATES: protein motif:HMM:NF015692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HIR3340731.1"
                     /translation="AWRWMRSSAPHPTGNPTPNEFSAYRCEFIKDHLRPMSVVSQPEP
                     YAKERAIVGCILGTAVGDAMGLACEGLSRRRQLKMFPELTGYKLLPGGKSLCSDDTEH
                     TCMLAQSLIGVAGLDADLAARQFASGFGWRLRFWLLGMPAGIGLATLRAIVKLWIGFP
                     ARYSGVYSAGNGPAMRAALIGVCHGDDPPRMRALASAATRITHTDPKAEHGALAVALA
                     AHLAAAGADIAPTDYARKLRELLGTGGGELAALADAVARSVDLKESAADYTARNGGAN
                     GVSGYMLHSVPAALHVWLGHQADYRGAVTATVRLGGDSDTVAAIVGAIIGARVGREGI
                     PSEWLENLWEWPRTTAWMERLGARLAASCSGRVNGSSLPLNWPGLLLRNALFIPLVLA
                     HGFRRLLPPY"
     gene            complement(1330..2313)
                     /locus_tag="AC158Q_12985"
     CDS             complement(1330..2313)
                     /locus_tag="AC158Q_12985"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3340732.1"
                     /translation="MSKNVSLRRFVTAVLAGSALLASTGIFPQSWPSKPVRVVVPFGT
                     GGGTDIQARLLAENLRKITGQNFITDNRSGAGGLIGAELVVNSPADGYTILFTTATLA
                     INTTLYSKTIKFNSQEDLVPSTLVSNAPNVLCVHPSVPAKSVKDLIAVAKKTPNGLNS
                     GVNTPGSTSHLAAEMLSQLTGIKTQIIAYTGGGPAMGGLMTGDIDLLFATGPVAAAAL
                     KTGRVRCLAVTTAEKNRSFPELPAIRTAVPGLEIGNWYGMFFPKGTPPESINRLSSII
                     KQALSDDKIKAFYAREGLEPVGSSPDQLKQQLAADITKYAKVIKAGNIPLR"
     gene            complement(2512..3480)
                     /locus_tag="AC158Q_12990"
     CDS             complement(2512..3480)
                     /locus_tag="AC158Q_12990"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3340733.1"
                     /translation="MKGRTLLTCTTIALGMALSGTSQAQNWPVKPVRIIVPFAPGGGT
                     DIQARLLGKNFFESMGQTFVVENRAGASGIIGAELAARSPADGYTILFTTASLAVNVT
                     LYGKRIKFDALKDLAPVSWISSTPLVLVVHPSVPVKSAKELVALAKKSGGLNAASNGA
                     GTTSHLSIEMLKQLTGVKVTHVPFKGGGPSMVSVVSGETDLLFATGPVASTQVKAGKV
                     RALAVTTAKKSSAFPELPTMTSIYPGLESDNWYAMFFPTGTPRPIIDKLNSEIAKALK
                     AKDIQDFMPREALDAVASSPEELHANFKREIARYAKIIQAGNIKLD"
     gene            complement(3534..>3845)
                     /locus_tag="AC158Q_12995"
     CDS             complement(3534..>3845)
                     /locus_tag="AC158Q_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3340734.1"
                     /translation="AFADQVLALDDSVPTGTYVDMCSRLPVVDPAKITAPTIIMRGQF
                     DGIAGFDDLTEFFKRLPNPDKQFAVMSGISHASFQQINYLLVYHILHSFFTQPEPIYR
                     G"
BASE COUNT          675 a         1224 c         1222 g          724 t
ORIGIN      
        1 ctgcctggcg ctggatgcgt tccagcgcgc cgcacccgac cggcaacccg acgccgaatg
       61 aattctcggc gtatcgctgc gaattcataa aagaccattt gcgcccaatg agcgtcgtca
      121 gccaacccga gccgtacgca aaggagcgag ccatcgttgg gtgcattctc ggcaccgcgg
      181 tgggcgatgc gatggggcta gcctgcgaag ggctgagccg cagacgacag ttgaaaatgt
      241 ttccggagtt gaccggttat aaactcttgc cggggggcaa gagtttatgc tccgacgaca
      301 ccgagcacac ctgcatgctc gcccaatcgc tgatcggggt cgcagggctg gacgccgacc
      361 ttgccgcgag acaattcgcg tccggcttcg gctggcggct gcgcttctgg ctgctgggaa
      421 tgcccgccgg catcggtctc gcgacattgc gcgcaatcgt gaaactgtgg atcggttttc
      481 cggcacgtta cagcggcgtt tactccgcgg gcaacgggcc ggcgatgcgg gccgcgctga
      541 tcggcgtgtg ccacggcgat gatccgcccc gcatgcgcgc gctcgcaagc gccgccacgc
      601 gcatcaccca caccgacccc aaagccgagc acggcgcgct cgcggtggcg ctcgccgcgc
      661 acctcgccgc cgcgggcgcg gatatcgcgc cgacagacta tgcacgaaag ctgcgcgagc
      721 tgctgggaac cgggggcggc gaactggccg cgctcgcgga tgccgttgcg cgcagcgtgg
      781 acctgaagga atccgcagcc gactacacgg cccgcaatgg cggtgcgaac ggggtgagcg
      841 gctatatgct gcactccgtt cccgccgcgc tgcacgtctg gctgggccat caagccgact
      901 atcgcggcgc ggtcacggcg acggtccgcc tcggcggcga ttccgatacg gtagcggcaa
      961 tcgtcggcgc catcatcggc gcgcgcgtgg gcagggaggg catcccgtcg gagtggctgg
     1021 agaacctgtg ggaatggccc cgcaccaccg cctggatgga gcggctcggc gcccggctgg
     1081 cggcgagctg cagcggacgc gtgaacggca gctccctgcc gctcaactgg ccggggctgc
     1141 tgctgcgcaa cgctctcttc atcccgctcg tgctggcgca cgggttccgg cggctgctcc
     1201 cgccctactg aagatgcgca agtcgtgatt cgtttatgcg cacatttccg acaaccgtac
     1261 aaaaaaagcg cccgcaagcg ggcgcttttt ttcccgggcc gcgagccatc ggctcgcacc
     1321 cggacccgct cagcgcaacg ggatgttgcc cgccttgatc acttttgcgt acttggtgat
     1381 gtcggccgcc agctgttgct tcagctggtc cggcgagctg ccgacgggtt ccaggccttc
     1441 ccgcgcgtaa aaggccttga tcttgtcgtc cgacagcgcc tgcttgatga tcgagctcaa
     1501 ccggttgatg ctctccggcg gcgtgccttt cgggaagaac atgccatacc agttgccgat
     1561 ttccaggccg gggaccgccg tccggatcgc aggcagttcc gggaacgaac ggtttttctc
     1621 cgccgtcgtc acggccaggc aacgcacgcg tcccgtcttc agggcggcgg cggcgaccgg
     1681 gcccgtggca aacagcaagt cgatgtcgcc cgtcatcagc ccgcccatcg ccggtccgcc
     1741 tccggtgtag gcaatgatct gcgtcttgat cccggtcaac tgcgacagca tctccgcggc
     1801 gagatggctg gtgctgccgg gcgtgttgac gccgctgttg agcccattgg gcgttttctt
     1861 tgcgaccgcg atcagatcct tgaccgattt cgccggcacg ctggggtgca cgcacagcac
     1921 gttcggcgcg ttggacacca gcgtggacgg caccaggtct tcctgggaat tgaacttgat
     1981 cgtcttggaa tacagcgtgg tattgatcgc gagcgtcgcc gtggtaaaca ggatcgtgta
     2041 gccatcggcg ggcgagttga cgaccagctc cgcgccgatc aggccgccgg cgccgctgcg
     2101 attatccgtg atgaagttct gacctgtgat ttttctcaga ttttcggcga gcaaccgtgc
     2161 ctggatgtcg gtgccgccgc cggtgccgaa cggcacgacg acgcgcaccg gcttggacgg
     2221 ccaggactgc gggaaaatcc cggttgaagc cagcaacgcc gaacccgcca gaacagccgt
     2281 gacgaaccgg cgcaaagaga cattcttgct catggaaacc ctcctcgttt ataggaatat
     2341 tggtggtacg gggatagggt tccactagtt ggactgtatt gcaacccctc ggcgggtcaa
     2401 aataaaatac tgtttaatat catagtgttg aataattaat gtgatgtcat gaaccagaaa
     2461 aaaggggcca cgggcccctt ttctcgcccc cccggcccgg ccggactgcc attagtcgag
     2521 cttgatgttg cccgcctgga tgatcttcgc gtagcgcgcg atttcgcgct tgaagttggc
     2581 gtgcaattcc tcgggactgc tcgccaccgc atcgagcgct tcccgcggca tgaagtcctg
     2641 gatgtctttc gccttcaggg ccttcgcgat ttccgaattg agcttgtcta tgatcggcct
     2701 gggcgttccc gtcgggaaaa acatggcgta ccagttatcc gactcgaggc cggggtaaat
     2761 ggaggtcatc gtcggcagtt cgggaaaagc cgaggatttc tttgccgtcg taactgccag
     2821 tgcgcgcacc ttgcccgcct tgacctgcgt ggacgccacc ggcccggtcg cgaacagcaa
     2881 atccgtctcg ccgctcacca ccgacaccat cgacgggcca ccgcccttga acgggacatg
     2941 cgtcaccttg acgccggtca gttgcttcag catctcgatc gagaggtggc tggtggtgcc
     3001 ggcgccgttg gacgcggcat tcaggccgcc ggacttcttc gcgagcgcaa ccagttcctt
     3061 cgccgatttc accggcacgc tcgggtggac gaccagaacg agcggcgtcg aggaaatcca
     3121 gctgaccggc gccaggtcct tgagcgcgtc aaacttgatc cgcttgccgt agagcgtgac
     3181 gttgaccgcc agcgacgcgg tggtgaacag tatcgtgtag ccgtccgccg gcgagcgcgc
     3241 agccagctcg gccccgatga tgccgctggc acccgcgcga ttttccacca cgaaggtctg
     3301 tcccatgctc tcgaaaaaat tcttgcccag caggcgggcc tgtatgtcgg tgccgccgcc
     3361 gggggcgaac ggcacgatga tgcgcaccgg cttgaccggc cagttctggg cctggctcgt
     3421 cccgctcaac gccatgccga gcgcaatggt cgtacaagtc aacaacgtcc ttcccttcat
     3481 cttccctcct ggtctgatgg atgaaaccga atgaaatgcg ttgccggcca gcctcagccg
     3541 cggtagatcg gctcgggctg ggtaaaaaac gaatgcagga tgtgatagac cagcaggtaa
     3601 ttgatctgct ggaagctcgc gtgcgagatt cccgacatca ccgcgaactg cttgtccggg
     3661 ttgggaaggc gcttgaagaa ttccgtaagg tcgtcgaatc cggcaatacc gtcgaactgg
     3721 ccgcgcatga tgatcgtggg cgccgtgatc ttcgcggggt ccaccaccgg cagccgggag
     3781 cacatatcga cgtaggtgcc ggtgggcacc gaatcgtcga gcgccagcac ctggtccgca
     3841 aacgc
//
LOCUS       DBNGSG010000491         4143 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_839396, whole genome shotgun sequence.
ACCESSION   DBNGSG010000491 DBNGSG010000000
VERSION     DBNGSG010000491.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4143)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_839396"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="AC158Q_13000"
     CDS             <1..410
                     /locus_tag="AC158Q_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340735.1"
                     /translation="PGSPAADHPDTTGETSGIDAAKSINRYWTLKGSTLNGTCTAAFN
                     YIDGIPVDRDSGATAASFIIRRGATCSGSGGGRTCTGWGLLTLSGTPNNTLATATGVS
                     ISSGDPESDFVVGEAAVTRFSREKEFIYTRELY"
     gene            529..1473
                     /locus_tag="AC158Q_13005"
     CDS             529..1473
                     /locus_tag="AC158Q_13005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="agglutinin biogenesis protein MshI"
                     /protein_id="HIR3340736.1"
                     /translation="MRLFGNKGRKPGWFCINLMPDRVDVCHVHATGRARPEILLCDSY
                     RKEGGDTATLARLRRELDLDSYRCTTLLRGGDYQVVQVDAPANVPEAEARGAVRWRIK
                     DMIDYPVEMATVDAVFIPAAGGTSGRQPQMLAVAAKNDVIAGAVKPFNDADIALEVID
                     IPELAQRNLAQRMELEGRGLALLAFNDQGGLLTFTCGGELYQSRRIDISLKSFDGASP
                     EQRKGLYDRLVLELQRSLDHFDRQFRHIAVARVMLTPAPGADDLRDHLAASLDVPVAL
                     IHLSEIMDFPHIPELHEDARQAQCLQLIGAAMREEAIA"
     gene            1577..2236
                     /locus_tag="AC158Q_13010"
     CDS             1577..2236
                     /locus_tag="AC158Q_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MSHA biogenesis protein MshI"
                     /protein_id="HIR3340737.1"
                     /translation="MSQNINLFNTAFRKQRQVLTLATVAQLLGITLVALFGYHYYLQQ
                     QVGGLAAELQGAEGLLKSQRGYVDRLKGRAAAPKADTQLEADIARYEAELKLARGSMD
                     ALKGGAFGNQQGFAEYLRAFSRQSVNGLWLTGFTIAGSGELEIRGRVLSPDLVPGYIQ
                     RLNKEQVLAGRSFARLEMNRPKPEAVANKNKDAKIVHRLPHFLEFSLATSDTARTEKM
                     P"
     gene            2233..2922
                     /locus_tag="AC158Q_13015"
     CDS             2233..2922
                     /locus_tag="AC158Q_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340738.1"
                     /translation="MKAQWRKLSARFGALKPRERVLVLLAAVVGTALVIDALALQPLE
                     ARKKRLTQQLAEARQNIKAADTLMQAQSAIADPDAVKRSYRDALRKQLAEIDKNMQGL
                     QRGLVPPERMAKLLEEMLTRSRGLQLVALRTLPAQRFEAPGTASSARPGDKAAKPAPG
                     DAERTIYQHSVEITLQGTYADLHDYLAQLEKLPSQMFWGRISVNTEQYPRLRVMLTVQ
                     TLSLNKAWLIV"
     gene            3006..3392
                     /locus_tag="AC158Q_13020"
     CDS             3006..3392
                     /locus_tag="AC158Q_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MSHA biogenesis protein MshK"
                     /protein_id="HIR3340739.1"
                     /translation="MQLAAALCLALSVPAAALAQVMNDPTRPPADMGAGAADDAVGGV
                     MLQSVMISPTQKAAIISGVMVKLGEKYGDAVLVRVSESEVVLKSGDAQQVLKLHPGVD
                     KREIAPAVEKGVQRRGKARAGSAQPR"
     gene            3406..>4143
                     /locus_tag="AC158Q_13025"
     CDS             3406..>4143
                     /locus_tag="AC158Q_13025"
                     /inference="COORDINATES: protein motif:HMM:NF019280.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HIR3340740.1"
                     /translation="MRLRTLMLAMAVAAAVGCAEQPGRRDAAYDRITGELDRAVKDRA
                     KPAAPDAVSQALLPPLVVEMPRVEGVKPLDQRFDLNVNNAPASQVFMAIVSGTRYSMI
                     VHPDVRDTLSVNLKDVTVMEALDTLRELYGYEYRVQGNRITVQPVTMQTRVFKVNYLQ
                     ASRSGRSDLRVSSGSITDAPTTPGGVPGAAPTPGTTGAPGQRLTESSRVTTSSESDFW
                     GDIVKSLSAIVGKADGRNVIVNSQSGVI"
BASE COUNT          785 a         1301 c         1380 g          677 t
ORIGIN      
        1 cgccgggctc gccggccgcc gatcatcccg atacgacagg ggagacatcg ggcatcgacg
       61 cagccaagag catcaaccgc tactggacgc taaagggttc gacgctcaac ggcacctgca
      121 ctgctgcctt caattatatc gacggcattc ccgtggaccg cgacagcggg gccacggccg
      181 cgagcttcat cattcggcgc ggcgccacct gcagcggcag cgggggcggc agaacctgca
      241 cgggctgggg tctgctgacc ttgagcggca cgccgaacaa tacgctggca acggcgacgg
      301 gcgtttcgat ttccagcggc gatccggaat ccgatttcgt ggtgggggag gcggccgtta
      361 cgcgcttctc gcgggagaag gagttcatct acacccgaga actttattaa acgtattagg
      421 ttgttatgca acaccgctgg atattttgct gaaaaaagcg ctccaacatc ttgacattat
      481 tgaattttat ggtattttca cccgattggt cgaaataggc ccagaacaat gcggttgttc
      541 ggcaacaagg gtcgaaaacc gggctggttc tgcatcaacc tgatgccgga ccgggtggac
      601 gtgtgccacg tccacgcgac cggccgggcg cgccccgaaa tattgctctg cgattcctac
      661 cgcaaagagg gcggcgatac ggcgaccctt gcccgcctgc ggcgcgagct cgacctcgac
      721 agctaccgct gcaccacgct gctgaggggc ggcgactacc aggtcgtgca ggtggacgcg
      781 cccgccaacg tgccggaggc ggaagcccgg ggcgcggtgc gctggcgcat caaggacatg
      841 atcgactatc cggtggagat ggcgacagtg gatgcggttt tcatccctgc ggcgggcggg
      901 acatcgggcc gtcagccgca gatgctggcg gtcgccgcga aaaacgacgt cattgccgga
      961 gccgtcaagc cgttcaacga cgccgacatt gcgctcgagg tgatcgacat cccggaactc
     1021 gcgcagcgca atcttgccca gcgcatggag ctggaaggcc gcggcctcgc cttgctggct
     1081 ttcaacgacc agggcggact gctgacgttc acctgtgggg gcgagctcta ccagtcccgc
     1141 cgcatcgata tttcgttgaa gagctttgac ggcgccagcc ccgagcagcg caagggtctc
     1201 tacgaccgtc tggtgcttga gctgcagcgc tcgctcgatc acttcgaccg ccagttccgc
     1261 cacatcgcgg tggcgcgggt gatgctgacg ccggcgcccg gcgcggacga cctgcgcgat
     1321 cacctcgccg ccagtctcga cgtgccggtg gcgttgatcc acctctctga gatcatggat
     1381 ttcccgcaca ttcccgagct gcacgaggac gccaggcagg cgcagtgcct gcagttgatc
     1441 ggcgcggcga tgagagagga ggcaatcgca tgatccgcct ccaatcgagt ttcgagttgc
     1501 cagttgccag tttcaagtta agccgtcgcc ggtatggaag cgccttcccg ctcgtgaaag
     1561 tgccgaaggc gccggcatga gccagaacat caacctgttc aataccgcgt tccgcaagca
     1621 gcggcaggtg ctgacactcg ccacggtggc gcaactcctc ggcatcacgc tcgttgcgtt
     1681 gttcggctac cactactacc tgcaacagca ggtcggcggc ctggccgcgg agctgcaggg
     1741 cgcggaaggc ctgctgaaat cgcagcgagg ttacgtggac aggctgaagg gcagggccgc
     1801 ggcgcccaag gccgatacgc aactggaagc cgacatcgcg cgctacgagg ccgaattgaa
     1861 actcgcgcgc ggctcgatgg atgcgctgaa gggcggcgca ttcggaaacc agcagggctt
     1921 cgcggagtac ctgcgtgcct tctcgcggca gtcggtgaac gggctgtggc tgaccggatt
     1981 caccatcgcc ggcagcggcg agctggagat ccgtggccgc gtgctctctc ccgatctcgt
     2041 gccaggctat atccagcgtc tgaacaagga acaggtgctg gcgggccgca gcttcgcgcg
     2101 gctggagatg aaccggccca agccggaggc cgtggcaaac aagaacaagg atgcgaagat
     2161 agtccaccgc cttccgcact ttctcgaatt cagcctggcc accagcgaca cggccaggac
     2221 ggaaaaaatg ccatgaaggc gcaatggcgg aagctctcgg cccggttcgg cgcgttgaag
     2281 ccgcgcgagc gcgtgctggt gctgcttgct gcggtcgtcg gcaccgcgct cgtcatcgat
     2341 gcactggcgc tgcagccgct ggaggcgcgg aaaaagcgcc tcacccagca gctcgcggaa
     2401 gcgcgccaga acatcaaggc cgcggacacc ctgatgcagg cgcaatcggc gatcgccgac
     2461 cccgatgccg tcaagcgttc gtaccgggac gcgctgcgca agcagcttgc cgagatcgac
     2521 aagaacatgc agggtttgca gcgcgggctg gtgccgcccg agcgcatggc aaagctgctc
     2581 gaagaaatgc tcacgcgcag ccgcgggctg caactcgttg cgctgcgcac cctgccggcg
     2641 cagcgcttcg aggccccggg cacggcgtcc tccgccaggc cgggcgacaa ggccgcgaaa
     2701 ccggcgcccg gggatgccga acgcaccatc tatcagcaca gcgtcgaaat cactctgcag
     2761 ggcacttatg cggacctgca cgattatctc gcgcagctcg agaagctgcc gtcgcagatg
     2821 ttctgggggc gcatcagcgt gaataccgaa caatacccca ggctgcgggt gatgttgacc
     2881 gttcagacgt tgagcctgaa caaggcctgg ctcatcgtat gaaacagttt cccgtttccg
     2941 gtttccggtt tccagttggg aaaaagggcg gtcggtgccc atactcgaaa ctcgaaactc
     3001 gaattttgca gctcgccgcc gcgctatgtt tggcgctgtc cgtgccggct gcggcgctgg
     3061 cgcaggtgat gaatgatccc acccgtccgc ccgctgacat gggtgccggc gcagccgatg
     3121 acgccgtcgg cggcgtgatg ctgcaatccg tgatgatttc gccaacgcag aaagcggcga
     3181 tcatcagcgg agtgatggtg aagctcggcg agaaatacgg cgatgccgtg ctggtcaggg
     3241 tatcggagag cgaagtcgtg ttgaagagcg gcgacgcgca gcaggtgctc aagctgcatc
     3301 cgggcgtgga caagcgcgag atcgcgcccg ccgtggagaa gggcgttcag cgccggggaa
     3361 aagcgcgggc gggctccgcg cagccccggt agaggaagga tgagtatgag actacgaaca
     3421 ttgatgctcg ccatggccgt cgcagccgct gtgggctgcg cggagcaacc ggggcggcgg
     3481 gatgcggcct acgaccggat caccggcgaa ctcgatcgcg ccgtgaagga tcgcgccaaa
     3541 ccggcggcgc cggacgcggt cagccaggcc ctgctgcccc cgctggtggt ggagatgccc
     3601 agggtggagg gcgtcaagcc gctcgaccag cgcttcgatc tcaacgtcaa caatgctccg
     3661 gccagccagg tgttcatggc gatcgtctcg ggcacgcgct acagcatgat cgtgcacccc
     3721 gacgtgcgcg acaccctgtc ggtgaacctg aaggacgtca ccgtgatgga ggcgctcgat
     3781 acgttgcgcg agctctacgg ctacgagtac cgggtgcagg gcaaccgcat cacggtgcag
     3841 ccggtgacca tgcagacccg cgtattcaag gtcaattacc tgcaggcgag ccggtccggg
     3901 cgcagcgatc tgcgggtgag ttccggctcg atcaccgatg cgccgacgac cccgggcggg
     3961 gtgcccggcg cggcgccgac cccgggcacg accggcgcgc cggggcagcg gctgaccgag
     4021 agcagccgcg tcacgacttc ctccgaaagc gatttctggg gcgacatcgt gaaatcgctc
     4081 tccgccatcg tcggcaaggc cgacgggcgc aacgtgatcg tcaactcgca atccggggtg
     4141 atc
//
LOCUS       DBNGSG010000492         5228 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3725989, whole genome shotgun sequence.
ACCESSION   DBNGSG010000492 DBNGSG010000000
VERSION     DBNGSG010000492.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5228)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3725989"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /locus_tag="AC158Q_13030"
     CDS             complement(<1..1145)
                     /locus_tag="AC158Q_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019042409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA];
                     GO_process: GO:0065002 - intracellular protein
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecD"
                     /protein_id="HIR3340741.1"
                     /translation="MNRYSLWQYLLIAGTLVIGLLYTLPNFYGEAPAVQVSPLRSSLK
                     ADDALRGRVEEALKKGNLAADGVFLDSASVKVRFADTDTQLKAKDLLAAQLGADYIVA
                     LNLLSNSPRWLTSIGALPMYLGLDLRGGVHFLLQVDMKAALAKKLEAFTNDIRGGLRE
                     KRIQYSGISRDGQTITVRFRDKATRDKAQNEIARTIPDLEFREADDAGEFRLITALKL
                     EAQRKTQEFAIQQNITTLRNRVNELGVAEPIIQQQGADRVVVQLPGVQDTAKAKDILG
                     RTATLEVRMVDDEASSNPAVLQAAQAGQVPFGDEFYIERGGAPILVRKPVVLTGDRIN
                     DAQPGFDNQTGEPAVHINLDGAGARIFHQVTRENVGRRMAIILFEKG"
     gene            complement(1235..1558)
                     /gene="yajC"
                     /locus_tag="AC158Q_13035"
     CDS             complement(1235..1558)
                     /gene="yajC"
                     /locus_tag="AC158Q_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HIR3340742.1"
                     /translation="MLINPAWAQGSGGAAGGGIESMVLIVLMFGVLYFLMIRPQMKRA
                     KEHKSMIDALQKGDEVITSGGVLGRISKINENYVTLEVSDKVEILVQRPAVQLVLPKG
                     TIKNI"
     gene            complement(1875..2996)
                     /gene="tgt"
                     /locus_tag="AC158Q_13040"
     CDS             complement(1875..2996)
                     /gene="tgt"
                     /locus_tag="AC158Q_13040"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HIR3340743.1"
                     /translation="MQFEVLANDGAARRGRLALPHGVVDTPAFMPVGTYGTVKAMSPQ
                     ELKALGAQIVLGNTFHLWLRPGLDVIGAHGGLHRFMGWDGPILTDSGGYQVYSLGDLR
                     KITEEGVRFQSPVNGDPCFLTPEESMRIQRVLNADIVMVFDECTPYPAEYDQARDSMR
                     LSLRWAARSKRAHDGNGNALFGIVQGGMHEPLRDESLAGLKEIGFDGYAIGGLSVGET
                     RREMTRVLRHTAPQLPADRPRYLMGVGTPQDLVAAVAAGIDMFDCVLPTRNARNGWVY
                     TRHGIIKLRNARHRDEVKPLDEECACYTCRHFTRAYLHHLQRVNEILGARLNTLHNLH
                     YYQELMSGLRAAIEGRRLAGFVDDFKGKMKPPMNADERG"
     gene            complement(3201..4232)
                     /gene="queA"
                     /locus_tag="AC158Q_13045"
     CDS             complement(3201..4232)
                     /gene="queA"
                     /locus_tag="AC158Q_13045"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HIR3340744.1"
                     /translation="MKLSDFDYDLPQELIAQFPSGVRGADRLLHLDGATGRISDRQFS
                     DFAGLVAPGDVVVLNDTRVIKARLTGKKQSGGHVEVMVERVLGGGEVLAQIGANHPTR
                     VGITLQLDGGVEATVLGREREFFHLRFEGCDDALALLDRHGSVPLPLYITRDAGDGDT
                     ERYQTIYARERGAVAAPTAGLHFDQAMLEALLGKGVALANITLHVGAGTFQPVRVQDL
                     AQHEMHGEWYDVPQATADAVNRAHSKGGRVLAVGTTTLRALESAAAGGKLKAGHDETR
                     LFILPGYRFRVVERLLTNFHLPKSTLLMLASAFGGMDNVRRAYRHAIEERYRFYSYGD
                     AMLIERTGK"
     gene            4605..4691
                     /locus_tag="AC158Q_13050"
     tRNA            4605..4691
                     /locus_tag="AC158Q_13050"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4639..4641,aa:Leu,seq:caa)
     gene            complement(4696..>5228)
                     /locus_tag="AC158Q_13055"
     CDS             complement(4696..>5228)
                     /locus_tag="AC158Q_13055"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-phosphopantoate--beta-alanine ligase"
                     /protein_id="HIR3340745.1"
                     /translation="VVEPSDLQHILEGAHRPGHFRGVATVVLKLFNLVMPHSAIFGKK
                     DYQQYLVLRDMVRQFALPIEIIPAETVRAPDGLALSSRNNYLLPAERKEAPRLYGVLK
                     KVRDEIGSGARDYQRLDQQAMAELARNGWQPDYVAVRRQADLRQCTERDRDLVIVAAA
                     RLGRTRLIDNLEVTLK"
BASE COUNT          935 a         1669 c         1587 g         1037 t
ORIGIN      
        1 ttgcctttct cgaacaggat gatcgccatg cgccggccga cgttctcgcg cgtgacctgg
       61 tggaagatgc gcgcgcctgc gccgtcgagg ttgatgtgca ccgcgggctc gccggtctgg
      121 ttgtcgaagc ccggctgcgc gtcgttgatg cggtcgccgg tcagcacgac cggctttctc
      181 accaggatcg gggcgccgcc gcgctcgatg tagaactcgt cgccgaaggg cacctgcccc
      241 gcctgcgccg cctggagcac cgccggattg ctgctcgcct cgtcgtccac catccgcact
      301 tcgagcgtcg ccgtgcgccc gaggatgtcc ttcgccttgg cggtatcctg cacgccgggc
      361 agctgcacca ccacgcggtc ggccccctgc tgctggatga tgggttccgc gacgcccagc
      421 tcgttgaccc ggttgcgcag cgtggtgatg ttctgctgga tcgcgaattc ctgtgtcttc
      481 ctctgcgctt cgagcttcag cgcggtgatg aggcgaaatt cgcccgcatc gtccgcctcc
      541 ctgaactcga gatccggtat ggtgcgggcg atttcgttct gtgccttgtc ccgcgtcgcc
      601 ttgtcccgga agcgcaccgt gatcgtctgg ccatcgcgcg agatgccgct gtactggatt
      661 cgcttctcgc gcaggccgcc gcggatatcg ttggtgaagg cttccagctt cttggcgagc
      721 gccgccttca tgtccacctg cagcaggaaa tgcacgccgc cgcgcagatc cagccccagg
      781 tacatcggca gcgcgccgat gctggtcaac cagcgcgggc tgttggaaag gaggttcagc
      841 gcgacgatgt agtccgcgcc gagctgcgcg gcgagcaggt ccttcgcctt cagctgggta
      901 tcggtgtcgg cgaagcgcac cttgacgctg gccgaatcga gaaacacgcc gtcggcggca
      961 agattgccct tcttgagcgc ttcctcgaca cggccccgga gggcatcgtc ggccttgagc
     1021 gacgagcgca acggcgacac ctgcaccgcc ggggcttccc cgtagaagtt gggcagcgta
     1081 tagagcaggc cgatgaccag cgtgcccgcg atgaggaggt actgccagag ggagtagcga
     1141 ttcatgcgag ggcgtgattc gtgaatcgtg aatcgtgaat cgtgaactcc atgcgacggt
     1201 tcagcgggag gatcgaatca caaatcacga atcactatat gttcttgatg gtgcccttcg
     1261 gcaacaccag ctgcaccgcc gggcgctgca cgagtatttc gactttgtcg gacacttcga
     1321 gggtcacgta gttctcgttg atcttgctga tgcggccgag caccccgccc gaagtgatca
     1381 cttcgtcgcc cttctgcagg gcgtcgatca tggacttgtg ctccttggcg cgtttcatct
     1441 gcggccggat catcaggaag tacagcaccc cgaacatcag cacgatcagg accatgctct
     1501 cgatgccgcc gccagccgcg ccgccggacc cctgggccca ggctggattg atcaacatgc
     1561 cagacatacg aattcgtctc cgaggactcg aaaaacgttg aattttaaca cgatttggcc
     1621 tgctttcgcc ttaacatcaa ttaagcaatg gacaggattt acaagattta caggatcgga
     1681 taaagaaaac aaaaaacact tggaaaatca ttggttgaat ctgattaatc ctgttaagga
     1741 tgctctgcaa aagtcgtttt tcgtcattcc cgcgaatgcg ggaatccagg gtaaacaaat
     1801 cgttactgga tccccgcctg cgcggggatg acttatgcag aggttcctta atcctgtcca
     1861 ttgaagtttt ggttctatcc gcgttcatcg gcgttcatcg gcggtttcat cttgcctttg
     1921 aagtcatcaa cgaaaccggc cagccggcgc ccctcgatcg cggcacgcag cccgctcatc
     1981 agttcctggt aatagtgcag gttatgcagc gtgttcaacc gcgcgcccag gatttcgttc
     2041 acgcgttgca ggtgatgcag gtaggcgcgc gtgaaatggc ggcaggtgta gcaggcgcat
     2101 tcctcgtcga gcggcttcac ctcgtcgcga tgacgcgcat tgcgcagctt gatgatgccg
     2161 tggcgcgtgt agacccagcc gttgcgcgcg ttgcgcgtcg gcagcacgca atcgaacatg
     2221 tcgatgccgg cggcgaccgc cgccacgagg tcctgcggcg tgcccacccc catgagatag
     2281 cgcgggcgat ccgccggcag ctgcggcgcc gtatgccgga ggacccgcgt catctcccgc
     2341 ctggtctcgc ccaccgacag cccgccgatg gcatagccgt caaatccgat ttccttcagc
     2401 cccgcgagcg actcgtcgcg cagcggctcg tgcatgccgc cctggacgat gccgaagagc
     2461 gcgttgccgt tgccgtcgtg cgcgcgtttc gagcgggctg cccacctcaa agaaagccgc
     2521 atggaatcgc gcgcctgatc gtattccgcc gggtacggag tgcactcgtc gaacaccatg
     2581 acgatatcgg cattgagcac gcgctgaatg cgcatggatt cctcgggcgt gagaaagcag
     2641 gggtccccgt tgaccggcga ctggaaacgc acgccctcct ccgtaatctt gcgcagatcg
     2701 ccgaggctgt acacctggta gccgcccgag tcggtcagga tgggtccgtc ccagcccatg
     2761 aagcggtgga gccccccgtg cgcgccgatg acatcgagcc ccggccgcag ccagaggtgg
     2821 aaggtgttgc cgagcacgat ctgcgcgcca agcgccttca attcctgcgg gctcatggcc
     2881 ttcaccgtgc cgtaggtgcc gacgggcatg aacgccggcg tgtccacgac gccgtgcggc
     2941 agcgcgagcc tcccccgccg cgccgcgccg tcgttcgcaa gcacctcgaa ctgcatgtct
     3001 aaaccctaaa ttagccgcca agacgccaag aacgccaagg aaatcacaat aacaaaaagg
     3061 aatattcaaa aactcaaatc aattcttgca tctcctgctt gacgatcccg gtcattcagt
     3121 tgaattttct ctgagtattg tttgctcttt attattgatc ttcttggcgg tcttggcgtc
     3181 ttggcggcta ttttccgatt ctactttccg gttctttcaa tcaacatcgc gtcgccgtaa
     3241 ctgtagaagc gatagcgctc ctcgatcgca tggcggtacg cgcggcgcac attatccatg
     3301 ccgccgaagg cggaagccag catgagcagc gtggacttcg gcaggtggaa gttggtgagc
     3361 aggcgctcga ccacgcgaaa gcggtagcca ggcaggatga acagccgtgt ttcgtcgtgt
     3421 ccggccttca atttcccgcc ggccgccgcg ctttccagcg cgcgcagcgt cgtcgtgccc
     3481 accgccagca cgcgcccgcc tttgctgtgc gcgcggttga ccgcgtccgc cgtcgcctgc
     3541 ggcacgtcat accactcgcc atgcatctcg tgctgcgcga ggtcctgcac gcgcaccggt
     3601 tgaaacgtgc cggcgccgac gtggagcgtg atattggcca gcgccacgcc cttacccagc
     3661 agcgcttcca gcatcgcctg gtcgaaatgc aatccggccg tcggcgcggc gaccgcgccg
     3721 cgctcgcgcg cgtagatcgt ctgatagcgc tccgtatcgc cgtctccagc atcgcgagtg
     3781 atatacagcg gcagcggcac gctgccgtgg cggtcgagca aagccagcgc atcgtcacag
     3841 ccctcgaaac gcaagtgaaa aaattcgcgc tcgcgtccga gcaccgttgc ctcgaccccg
     3901 ccgtcgagct gcagggtaat ccccactcga gtcggatggt tcgccccgat ctgcgccagg
     3961 acttcgccgc cgccgagcac gcgctcgacc atcacttcca catgcccgcc gctctgcttc
     4021 tttccggtga gccgcgcctt gatcacgcgg gtgtcattca gcacgacgac atcgccgggc
     4081 gcaacgagcc cggcgaaatc gctgaactgg cggtctgaga tcctccccgt cgcgccatcg
     4141 aggtgcagca gccggtcggc gccgcgcacg ccggatggaa actgcgcgat caactcctgc
     4201 ggcaggtcgt agtcgaaatc ggaaagcttc aagattcagc cacagagaac acagagaaca
     4261 cagaggataa acccaaactc gagttcgtcg ggagcttgat ccgcgtgaat ttcttggcgg
     4321 ccttggcggt tcaattatcg attttgtttt tctctgtgct ctctgtggct atgttgttag
     4381 gggtgggggt ttgcgggata atttgcctgc attcctgccg ggatggcgga attggtagac
     4441 gcacgggact cacaacaggc cgatgtcatt gatgtccgtg acgcgcagcg tcacgttgca
     4501 attgcggtcg gcctgttgcg gcgcaggcta acttgcctgc aagttaagcg ccgcaggcgc
     4561 ggctggagcc aaaatcccgc gcccgtttgc tagcatacgc acgtgccggg atggcggaat
     4621 tggtagacgc acgggactca aaatcccgcg gtggcaacac catgagagtt cgattctctc
     4681 tcccggcacc accggctact ttagggttac ctcaaggttg tcgatgagcc gcgtgcggcc
     4741 gagccgcgcg gcggcgacga tgaccagatc gcggtcacgc tccgtgcact ggcgcagatc
     4801 ggcctgccgg cgcaccgcga cgtaatcggg ctgccagccg ttacgcgcca gttcggccat
     4861 cgcctgctga tcgaggcgct gatagtcccg cgctccgctc ccgatctcgt cgcgcacctt
     4921 cttcagcacg ccgtagagcc gcggcgcttc cttgcgctcc gccggcaaga ggtagttgtt
     4981 gcgcgaagac agcgccaggc cgtccggagc gcgcacggtc tcggcaggga tgatctcgat
     5041 cggcagcgcg aactgcctca ccatgtcgcg cagcacgagg tactgctggt aatccttctt
     5101 gccgaaaatc gccgagtgcg gcatcacgag gttgaagagc ttcaggacca cggtggcgac
     5161 gccgcggaaa tgcccgggcc ggtgcgcgcc ttccagtatg tgctggagat cggagggctc
     5221 gaccacgt
//
LOCUS       DBNGSG010000493         5718 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1519800, whole genome shotgun sequence.
ACCESSION   DBNGSG010000493 DBNGSG010000000
VERSION     DBNGSG010000493.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5718)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5718)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5718
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1519800"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="AC158Q_13060"
     CDS             <1..212
                     /locus_tag="AC158Q_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340746.1"
                     /translation="LSALTVDVGVGSALSSGVTNIAFTGGVLQIDLSGNGSFEAADDF
                     QIAVTGANTVTYDSESGTFTLSSVE"
     gene            437..1387
                     /locus_tag="AC158Q_13065"
     CDS             437..1387
                     /locus_tag="AC158Q_13065"
                     /inference="COORDINATES: protein motif:HMM:TIGR03508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DmsE family decaheme c-type cytochrome"
                     /protein_id="HIR3340747.1"
                     /translation="MQALVARRVRRTAAARGAYAALALAGVLACAPAAAQDPDGYVGE
                     ARCAACHKLEQEHWAHTVHASAFRSNQRNALAARGCEACHGPGAKHLLDAADKSAIVA
                     FTRRRGVPVETQNAQCLQCHQGRSRIFWQGSVHETSRLGCSDCHNPMAKFSASGLLAK
                     EGVSETCYACHSQQRAEFRRRSHMPLPEGRLSCGDCHNPHGSVTRPLIKADSVNQLCY
                     ACHAEKRGPFIWEHAPVRRDCMSCHVPHGSSHESLLAVARPFLCQQCHSSVSHPASLL
                     TRPNLPRGAIPDERAANRGCSNCHSQVHGSNHPSGARFQR"
     gene            1404..3887
                     /locus_tag="AC158Q_13070"
     CDS             1404..3887
                     /locus_tag="AC158Q_13070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtrB/PioB family decaheme-associated outer
                     membrane protein"
                     /protein_id="HIR3340748.1"
                     /translation="MREEGGETTRPGVIARLILGPVALWAALSLPVCHGALAQSHSGA
                     AQASAYNRAGTDPTLERDERGLSQLLPERSRSPGGWLYDAPYEMRPLLPLGGDWNYRV
                     SAEFGAVHGTGGAPRLRGYGDYRNGAVLNYFSLGLEQSGAPNYLDFTAGAAGREDQYY
                     RASFGRRGDFRASLYFTETPKLFTDRARTVFLGAGSGNLTLPAGLVPGNNTPAQLAAA
                     LAAAPLLELGFSRRTSGLEFDATPGGDWRIYARYAQDRKHGTRPFGGASGYPGAAAVE
                     TIEPLDHKTHNLLAGLQWSGAALQVNLAYTGSFFRNAVGTLTWEHPLAVGDPAVVQRG
                     RMDLHPDNASHNVKLDWSAALPLRGRFTGGLSWGRMTQDDALITPTVNAGVLGATDLS
                     DWNTPAALSRASADARIDTRLAHLGAAFSPLDDLSLRLKLRHYEEDNRTRYSAFNPLT
                     GQFGYLGLDGANNFDIVPGLFRVPLAAVPFERRKDNYGVEADWRLLRRTSVTLGYERE
                     NSRDPYRAVAASAEDRVRAGLNNRDLPWATARLSVEHANRSGDGYVYDPNRLLYGAGV
                     INAPAMLAALRQHDIADRRQSVVNARVNFLVARDMDLGVAGRYLDNDYGAAYGRLGER
                     KSALNLEWNWQPRPGASAWAHYGFERTRNRMAQIGDDPAGWASGDPAAGGPAYPLANR
                     WEEESRDDAHVLGAGLRYAFSRATLESAYTYLYSPYRTRYAFASAGALASGAAAAAGA
                     GDGLPDMVFRSQALETSLRVRLGRDTALRLYHRYERARFEDWHYDGLPLILGSGEAVV
                     LGAGPVSYSTNLIGVFYQYAPGMRDRAAR"
     gene            4009..>5718
                     /locus_tag="AC158Q_13075"
     CDS             4009..>5718
                     /locus_tag="AC158Q_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013207171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HIR3340749.1"
                     /translation="MAFHAWDINEVYSNADGTIQFIELFNNTAGTGENVLDGLTITFT
                     PTGEDTVTYTIPTPDNFSGNTAFKTLLFGTQAYADLEGVPDPDFIIPSGFLSVTGGTL
                     DLFPDSDLGLIDTLTFASLPTDGTQSLTGTGAIQSVNSPRNFAGETGTVVLADPDDLI
                     IGTAGADSLDGADGDDTLDGLAGNDTLLGGTGNDSLSGGDDNDQVFGNQDNDTLSGGA
                     GVDSLYGGQGNDIVSAGDGNDYVEGNLGDDVMAADAGNDTMHGGQGNDNLTGGDGNDS
                     LVGGAGVDAVFGNLGDDTLAGGADADSLYGGQNNDAVNGGDGNDYAEGNLGDDVMAGD
                     AGNDTMHGGQGNDFFDGGLGNDSLVGGLGNDTLYGGVGDDTAGQGDDTLIGGDGDDTL
                     DGGEGSDVLSGGAGVDTLNGGNGNELMTGGLGNDLLIGGFGMDTLVGGDGTGLDTADG
                     GDFLDGGDNEDSLVGGHGNDTLAGGAGLDTLFGNLGDDSLAGDADGDSLFGGQGNDAV
                     SGGDGNDYAEGNLGDDVMAGEAGNDTMHGGQGNDTLEGGTGNDSLVGGLGDDYLLGGL
                     DTLG"
BASE COUNT          904 a         2057 c         1996 g          761 t
ORIGIN      
        1 cgctctccgc gctgacggtg gacgtggggg tgggcagcgc gctctcctcg ggcgtcacca
       61 acatcgcgtt cacgggcggg gtgctgcaga tcgatttgag cggcaacggc agcttcgagg
      121 ccgcggacga tttccagatc gcagtgaccg gcgccaacac ggtaacctac gactcggaga
      181 gcggcacgtt caccctgtcg tccgtggagt aatcatccgc aacgggcggg ggcagcatcc
      241 cccgtcccga tccggaaagc gcagttcgaa agcggccgcc accagcggcc gttttttttc
      301 ggctgcgccc gccggggccg gcgtttcacg atccggttgc cgggcccgcg gcacggccat
      361 gccggcggaa taaaacgcgc tgcccccgcg ttggtcgtgg gaggggggcc ggcggcgcag
      421 tcgccggaac gacatcatgc aggctcttgt tgcccggagg gttcgccgga cagccgccgc
      481 gcgcggcgcg tacgcggcgc tcgctctcgc cggcgtgctc gcgtgtgcgc cggcggccgc
      541 acaggacccg gacggctacg tgggcgaggc gcgctgcgcc gcgtgccaca agctcgagca
      601 ggagcactgg gcgcacacgg tccacgccag tgctttccgg tcgaatcaac gcaacgcgct
      661 cgccgcgcgc ggctgcgagg cctgccacgg gcccggcgcg aaacacctcc tcgacgccgc
      721 cgacaagtcc gccatcgtcg ctttcacccg ccgccgcggc gtgccggtgg aaacccagaa
      781 cgcgcagtgc ctgcagtgcc accagggccg cagccgcatc ttctggcagg ggtccgtcca
      841 cgagacgagc cggctcggct gcagcgactg ccacaacccg atggcgaaat tctccgcgtc
      901 ggggctgctg gcgaaggagg gcgtctccga aacctgctac gcgtgccact cgcagcagcg
      961 cgcggaattc cgccgccgct cgcacatgcc gctgccggag ggcaggctct cctgcggcga
     1021 ctgccacaac ccgcacggct cggtcacgcg gccgctgatc aaggcggaca gcgtgaacca
     1081 gctctgctac gcctgccacg ccgagaagcg cggccccttc atctgggagc acgcgccggt
     1141 gcggcgcgac tgcatgtcgt gccacgtgcc gcacggctcc agccacgaga gcctgctcgc
     1201 ggtggcgcgg ccgttcctgt gccagcagtg ccactccagc gtctcgcacc ccgcgagcct
     1261 gctcacgcgg ccgaacctgc cgcgcggcgc gattcccgac gagcgcgctg cgaaccgcgg
     1321 ctgcagcaac tgccactcgc aggtgcatgg ctccaaccac ccgtccggcg cgcgcttcca
     1381 gaggtgatga tgagggaggt gggatgaggg aagagggagg ggaaacaacc cgtccgggcg
     1441 tgattgcgcg gctgatcctg ggaccggttg cattatgggc cgcgctgtcg ctgcccgtct
     1501 gccacggcgc gctcgcgcag tcgcattcgg gggcggccca ggcaagcgca tacaaccgcg
     1561 cgggaaccga ccccacgctg gagcgcgacg agcgcggcct gtcgcaactg cttccggagc
     1621 gctcgcgctc gcccggcggc tggctctacg acgcgcccta cgagatgcgc ccgctgctgc
     1681 cgctcggggg cgactggaac taccgcgttt ccgccgagtt cggcgccgtg cacggcactg
     1741 gcggcgcgcc gcggctgcgc ggctacggcg actaccgcaa cggcgccgtg ctcaattatt
     1801 tcagcctcgg gctggagcag tccggcgcgc cgaactacct ggacttcacc gccggcgcgg
     1861 ccgggcgcga ggatcagtat taccgcgcga gcttcggccg ccgcggcgat ttccgcgcga
     1921 gcctgtattt caccgagacg ccgaagctct tcaccgaccg cgcgcgcacc gtattcctcg
     1981 gcgccggcag cggcaatctc acgctccccg ccgggctcgt gccgggcaac aacacgcccg
     2041 cgcagctcgc cgccgcgctc gcggcggcgc cgctgctcga gctggggttc tcgcgcagga
     2101 cttccgggct cgagttcgac gcgacgccgg gcggcgactg gcggatctac gcccgctacg
     2161 cgcaggaccg caagcacggc acgcgcccct tcggcggcgc ctccggctat cccggcgcgg
     2221 ctgcggtgga gacgatcgag ccgctcgacc acaagaccca caacctgctg gcgggcctgc
     2281 agtggagcgg cgccgcgctg caggtgaacc tcgcctacac cggctccttt ttccgcaacg
     2341 ccgtcggcac gctgacctgg gagcatccgc tcgcggtggg ggaccccgcc gtcgtgcagc
     2401 gcgggcgcat ggaccttcac cccgacaacg cctcccataa cgtgaagctc gactggagcg
     2461 cggcgctgcc gctgcgcggg cgcttcaccg gcggcctgtc gtggggccgc atgacccagg
     2521 acgacgcgct gatcacgccc accgtgaatg ccggcgtgct gggcgcgacc gacctttcgg
     2581 actggaacac gcccgccgcc ctgagccggg cgagcgcgga cgcccgcatc gacacccggc
     2641 tcgcccacct cggcgcggcg ttctcgccgc tcgacgacct ctcgctgcgg ctgaagctgc
     2701 ggcactacga ggaagacaac aggacccgct acagcgcgtt caacccgctg accgggcagt
     2761 tcggctacct cgggctcgac ggcgccaaca acttcgacat cgtgccgggc ctgtttcgcg
     2821 tgccgctggc agccgtcccg ttcgagcgcc gcaaggacaa ctacggcgtg gaggccgact
     2881 ggcggctcct gcgccgcacc agcgtcacgc tcggctacga gcgcgagaac agccgcgatc
     2941 cctaccgcgc ggtggcggcg agcgcggagg accgggtgcg cgcggggctc aacaaccgcg
     3001 acctgccgtg ggcgaccgcg cgcctctccg tggagcacgc gaaccgctcg ggcgacggct
     3061 atgtttacga ccccaaccgg ctgctctacg gcgccggggt catcaacgcg ccggccatgc
     3121 tcgccgcgct gcgccagcac gatatcgccg accgccgcca aagcgtcgtc aatgcccgcg
     3181 tcaatttcct ggtcgcgcgc gacatggacc tgggcgtggc gggccgctac ctcgacaacg
     3241 actacggcgc ggcgtacggg cggctgggcg agcgcaagtc cgcgctcaac ctggaatgga
     3301 actggcagcc gcgcccgggc gcctcggcct gggcccatta cggcttcgag cgcacccgca
     3361 accgcatggc gcagatcggg gatgacccgg ccggatgggc gagcggcgac cccgccgccg
     3421 gcggccccgc ctatccgctc gccaaccgct gggaggagga atcgcgcgac gacgcgcacg
     3481 tgctgggcgc gggcctgcgc tacgccttct cccgcgcgac cctggagagc gcctatacct
     3541 atctgtacag cccgtaccgc acgcgctacg ccttcgcctc cgccggcgcg ctcgcaagcg
     3601 gcgcggccgc ggcggcgggc gcgggcgacg gcctgcccga catggtgttc cgcagccagg
     3661 cgctggagac gagcttgaga gttcggctcg ggcgcgacac ggcgctgcgc ctctaccacc
     3721 gctacgagcg cgcgcgcttc gaggactggc actacgacgg gctgccgctg atactcggat
     3781 ccggcgaggc ggtggtcctc ggcgcgggcc ccgtgagcta ttccacgaac ctcatcgggg
     3841 tgttctacca gtacgctccc gggatgcggg atagggccgc gcgctgagcc gccgcatttc
     3901 cggggacgtc gcggctgggg catactcccg gaatagctgc ccgcccgcgg gcgtttaccg
     3961 aacgataagc cgtggccgcc gggccaccga taattcgagg gggacacaat ggcattccac
     4021 gcgtgggaca tcaacgaggt ctattccaac gccgacggca cgatccagtt catcgaactg
     4081 ttcaataaca ccgccggtac tggcgagaac gtcctcgacg gactcacgat caccttcacc
     4141 ccgaccggtg aagacacggt cacttacacg atccccacgc ctgacaattt cagcgggaac
     4201 acggccttca agacgctgct gttcggcacc caggcctacg ccgacctgga gggcgtcccc
     4261 gatcccgact tcatcatccc cagcggtttc ctctccgtca ccggcgggac gctggatctc
     4321 tttcctgatt ctgacctggg cttgattgac actttgactt tcgcatcatt gcctaccgac
     4381 ggcacgcagt cgctgaccgg caccggcgcc atccagtccg tcaactcgcc caggaatttc
     4441 gccggcgaaa ccgggacggt cgtgctcgcc gatcccgacg atttgatcat cggcacggcc
     4501 ggtgccgaca gcctcgacgg cgcggacggc gacgacacgc tcgacggcct cgccggcaac
     4561 gacacgctcc tcggcggcac cgggaacgac agcctctccg gcggcgacga caacgaccag
     4621 gtcttcggca accaggacaa cgacaccctc tccggcgggg ccggcgtgga cagcctctac
     4681 ggcggccagg gcaacgacat cgtgagcgcg ggcgacggca acgactacgt ggagggcaac
     4741 ctcggcgacg acgtgatggc cgcggacgcg ggcaacgaca ccatgcacgg cggccagggc
     4801 aacgacaacc tgaccggcgg cgacggcaac gacagcctgg tgggcggggc gggcgtggac
     4861 gcggtgttcg gcaacctggg cgatgacacc ctggcgggcg gcgccgacgc cgacagcctg
     4921 tacggcggcc agaacaacga cgcggtcaac ggcggggacg gcaacgacta cgcggaaggc
     4981 aacctcggcg acgacgtgat ggcgggcgac gccggcaacg acacgatgca cggcggccag
     5041 ggcaacgact ttttcgacgg tgggctgggt aacgacagcc tggtgggcgg cctgggcaac
     5101 gacacgctct acggcggcgt gggcgacgac accgcgggcc agggcgacga cacgctgatc
     5161 ggcggcgacg gcgacgacac gctggacggc ggcgaaggca gcgacgtgct ctccggcggc
     5221 gcgggcgtgg acacgctgaa cggcggcaac ggcaacgagc tgatgaccgg cgggctgggg
     5281 aacgacctgc tgatcggcgg cttcggcatg gacacgctgg tgggcggcga cggcaccgga
     5341 ctcgatacgg cggacggcgg tgatttcctc gacggcggcg acaacgagga ttcgctcgtg
     5401 ggcggccacg gcaacgatac gctcgccggc ggcgcgggcc tggacacgct gttcggcaac
     5461 ctgggcgacg actcgctggc gggcgatgct gacggggact ccctgttcgg aggccagggc
     5521 aacgacgcgg tgagcggcgg ggacggcaac gactacgccg agggcaacct cggcgatgac
     5581 gtgatggcgg gcgaggccgg caacgacacg atgcacggcg ggcagggcaa tgacacgttg
     5641 gagggcggca ccggcaacga cagcctggtg ggcgggctcg gcgacgacta cctgctcggc
     5701 ggtctggaca ccctgggc
//
LOCUS       DBNGSG010000494         3897 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3895987, whole genome shotgun sequence.
ACCESSION   DBNGSG010000494 DBNGSG010000000
VERSION     DBNGSG010000494.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3895987"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="AC158Q_13080"
     CDS             complement(<1..339)
                     /locus_tag="AC158Q_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR3340750.1"
                     /translation="MADTRTIEIEVLRYNPEKDAEPHFQTFEVPFTHDTSVLQGLQHI
                     KDHLDGSLTFRWSCRMAICGSCGKMVNGKPELSCHTFLRDYYPGKVRIEPLNHFPIVR
                     DLVIDQGDFFD"
     gene            complement(415..2184)
                     /gene="frdA"
                     /locus_tag="AC158Q_13085"
     CDS             complement(415..2184)
                     /gene="frdA"
                     /locus_tag="AC158Q_13085"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HIR3340751.1"
                     /translation="MQSVQTDVVVVGGGGAGLRAAIAVAESDPRLRVALVSKVVPMRS
                     HTVAAEGGSAGVIRTDDSLDHHFDDTVGGSDWLCDQDVVEYFVAHCTEEMVQLEHWGC
                     PWSRKDDGHVNVRFFGGMKVQRTWFAADKTGFHMLHTLFQTSHKYSSIKRFDEYFCAD
                     LVVEDGRVQGVVAIEVATGEFFLIECKAAIICTGGAGRVFGQNTNAGIVTGDGMGIAY
                     RHGVPLRDMEFVQWHPTALPGTGVLITEGCRGEGGILTNKDGYRYLQDYGLGPPDPWP
                     RPKAMELGPRDRLSQAFWHEEQKGRTVATALGNAVWLDLRHLGAAKIHERLPMITEVA
                     KVFAGVDPVVSPIPVRPAVHYTMGGILCNGQTETPLKGLYAAGECSSVGIHGANRLGS
                     NSLAEIVVFGKVAGERAAQYVKSAPSGRSDALHKQASAVERRLLGMLGQDKGDRVASI
                     RDEMHTTMETGVGIFRKADSMQVACSKLATLRDRLQRGIKLDDRNRVFNTEWLSAIEL
                     GFTLEIAEVIAQSALHRKESRGAHTRLDEFKARDDANFLKHTLAYRTGDGPPRLEYAP
                     VTITKSPPKTRTYGGEGKKVELT"
     gene            complement(2282..3799)
                     /locus_tag="AC158Q_13090"
     CDS             complement(2282..3799)
                     /locus_tag="AC158Q_13090"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006091 - generation of precursor
                     metabolites and energy [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HIR3340752.1"
                     /translation="MATIKQHDFIQSIADAFQFISFYHPVDFITAMGKAYEGEQSPAA
                     KNAIAQILMNSRMSAEGHRPVCQDTGIAVVFLKIGMNVRWEATMSVQEMVDEGVRRAY
                     TDKDNPLRASVLADPDGARKNTRDNSPAVVHMEVVPGDRVEVKLAAKGGGSESKSRFA
                     VLNPSDDVIEWILGQIPTMGGDWCPPGMLGVGIGGTPEKAMVLAKESLMAHIDINELK
                     QRGAKNRAEELRLELFEKVNALGIGAQGLGGLATVLDVKVLDYPTHAVMKPVAIVPNC
                     AATRHLEFHLDGSGPARFEAPSLDNWPKIAIDLAAGAKRVDLEKLTKAETATWKAGDR
                     LLLTGKILTGRDAAHKRLTDLMAKGEKLPVDLTNRFIYYVGPVDAVRDEVVGPAGPTT
                     SRRMDKFMDAMLGKTGMLGSIGKSERGADAVKVIKQHKGVHLIAIGGAAYLVAKAIRG
                     AKVLAFKDLGMEAIHEFEVKDMPVTVAVDASGNAVHTTGPAEWRAKIGKIPVAVV"
BASE COUNT          614 a         1285 c         1230 g          768 t
ORIGIN      
        1 gtcgaagaaa tcgccctggt cgatcaccag gtcgcgcacg atagggaaat gattcagcgg
       61 ctcgatgcgg accttgccgg ggtagtaatc gcgcaggaac gtgtgacagg agagttcagg
      121 cttgccgttc accatcttgc cgcagctgcc gcagatcgcc atgcggcacg accagcggaa
      181 ggtcaggctg ccgtcgagat ggtccttgat gtgctgcagc ccctgcagca ccgacgtatc
      241 gtgggtaaac ggcacctcga acgtctggaa atgcggctcc gcatccttct cggggttgta
      301 gcgcagtact tcgatttcga ttgtgcgggt atcggccatg cgcagtccct caatttccac
      361 tcagtttcga ctcagtttcc actcagtttc aactcagttt caactcagtt tcaattaggt
      421 caattcgact ttcttgcctt ctccgccata ggtccgcgtc ttgggcgggg acttggtaat
      481 ggtcaccggc gcgtactcga gccgcggcgg tccatcgccg gtgcggtagg caagggtgtg
      541 cttcaggaaa ttcgcgtcat cgcgcgcctt gaactcgtcg aggcgcgtat gcgcaccgcg
      601 ggattccttt cgatggagcg ccgactgagc gatcacctcg gctatctcga gcgtgaagcc
      661 gagttcgatc gccgacagcc actcggtgtt gaacacgcgg ttgcggtcgt cgagcttgat
      721 gccgcgctgg aggcggtcgc gcagcgttgc cagcttcgag cacgccacct gcatgctgtc
      781 ggccttgcgg aaaatgccga ccccggtttc catggtcgtg tgcatctcat ctcggatcga
      841 cgcgacacga tcgcccttgt cctgtccaag catcccgagc agccggcgct cgacggcgct
      901 cgcctgcttg tgcaacgcgt cggaccggcc ggagggcgcg gacttcacgt actgtgccgc
      961 gcgctcgccg gccaccttgc cgaacaccac gatttccgcg agcgaattgg agcccaggcg
     1021 gttggcgccg tggatgccga cgctcgagca ttcgccggcc gcataaagcc ccttgagcgg
     1081 cgtctcggtc tggccgttgc agaggatgcc gcccatcgtg tagtgcacgg cggggcgaac
     1141 cggtatcggg gagaccacgg gatcgacacc ggcaaaaacc ttcgccacct cggtgatcat
     1201 cggaagccgc tcgtggatct tggcggcgcc gagatgccgc aggtcgagcc acaccgcgtt
     1261 cccgagggcg gtggcgaccg tgcgtccctt ctgctcctcg tgccagaatg cctgcgacag
     1321 gcggtcgcgg gggccgagct ccattgcctt cgggcgcggc cacggatcgg gcggcccaag
     1381 gccgtagtcc tgaaggtagc gatacccgtc cttgttggtg agtatgccgc cttcaccgcg
     1441 acagccttcc gtgatgagca cgccggtgcc gggcagggcg gtcggatgcc attggacgaa
     1501 ctccatgtcg cgcaggggca cgccgtgccg gtaggcgatg cccatgccgt cgccggtgac
     1561 gatgccggca ttggtgttct ggccgaatac gcgtcccgcg cccccggtgc agatgatcgc
     1621 cgccttgcac tcgatcagga agaactcccc ggtcgcgacc tcgatcgcga ccacgccctg
     1681 cacccggccg tcctccacga ccaggtcggc gcagaagtat tcgtcaaagc gcttgatcga
     1741 ggagtacttg tgggaagtct ggaacagcgt gtgcaacatg tggaagccgg tcttgtccgc
     1801 ggcgaaccac gtgcgctgga ctttcatgcc gccgaagaat cggacgttga cgtggccgtc
     1861 gtccttgcgg ctccaagggc agccccagtg ctcgagctgg accatttcct cggtgcagtg
     1921 cgcgacgaaa tactcgacga cgtcctggtc gcacagccag tcgctgccgc cgacggtgtc
     1981 gtcgaaatga tggtcgagac tgtcgtcggt gcggatgacg ccggccgatc cgccctcagc
     2041 agccacggta tggctgcgca tgggaacgac cttcgagacc agcgcaacgc gaagccgcgg
     2101 gtcgctttcg gccaccgcga tcgccgcgcg cagtcccgcg ccgccgccgc caactaccac
     2161 gacatccgtc tgtaccgatt gcatctagtt ccccttcttg ccgtcggaca ctctcatgac
     2221 attctgacgt ctacatgttc tctgccttga tcccaatcaa gtatgtccgg caccacgtcc
     2281 tttagacgac cgcgaccgga atcttcccga tcttcgcgcg ccattccgcg gggccggtcg
     2341 tgtgcacggc attgccgctc gcgtccacgg ccacggtgac cggcatgtcc ttgacctcga
     2401 attcgtggat cgcttccatc ccgaggtcct tgaacgcgag gaccttcgcg ccgcggatgg
     2461 ccttggcgac gaggtaggcc gcgccgccta tcgcgatgag gtgaaccccc ttgtgctgct
     2521 tgatcacctt caccgcgtcc gcgccgcgct ccgatttccc gatggagccc agcatcccgg
     2581 tcttgccgag catggcgtcc atgaacttgt ccatgcggcg ggacgtggtg gggccggcgg
     2641 ggcccacgac ctcgtctcgc accgcgtcca ccgggccgac gtagtagatg aaccggttgg
     2701 tgaggtccac cggcagtttc tcgcccttgg ccatcaggtc ggtcagccgc ttgtgcgcgg
     2761 cgtcgcggcc ggtgaggatc ttgccggtca gcaggaggcg gtcgccggcc ttccaggtcg
     2821 cggtctcggc cttggtgagc ttctcgaggt ccacgcgctt cgcgccggcg gcaagatcga
     2881 tggcgatctt gggccagttg tcgaggctcg gcgcctcgaa acgggccggg ccgctgccgt
     2941 cgaggtggaa ctcgaggtgc cgggtcgccg cgcagttcgg cacgatcgcc accggcttca
     3001 tcaccgcgtg cgtgggatag tcgagcacct tgacgtcgag gaccgtggcg agcccgccga
     3061 gaccctgcgc gccgatgccg agggcgttga ccttctcgaa cagttcgagc cgcagctcct
     3121 cggcgcggtt tttcgcgccg cgctgcttga gctcgttgat gtcgatgtgc gccatcagcg
     3181 attccttggc cagcaccatc gctttctccg gggtgccgcc gatgccgacg cccaacatgc
     3241 cgggcgggca ccagtcgccg cccatcgtcg gtatctgccc gagtatccat tcgatcacat
     3301 cgtcgctggg attgagcacc gcgaagcgcg acttgctctc ggagccgccg cctttcgcgg
     3361 cgagcttcac ctccacgcgg tcgccaggga ccacctccat gtgcaccacg gccggcgaat
     3421 tgtctcgggt attcttgcgc gcgccgtccg ggtccgccag caccgaagcc cgcaacgggt
     3481 tgtccttgtc ggtgtaggcg cggcgcacgc cctcgtctac catttcctgg acgctcatcg
     3541 tggcttccca gcgcacgttc atgccgatct tgaggaacac cacggcgatg ccggtgtcct
     3601 ggcacaccgg gcggtggccc tcggccgaca tccggctgtt catgaggatc tgcgcgatgg
     3661 cgttcttcgc ggcgggcgat tgctcccctt cataggcctt gcccatggcg gtgatgaaat
     3721 ccaccgggtg gtagaaggag atgaactgga acgcgtccgc gatgctctgg atgaaatcgt
     3781 gctgcttgat ggtggccatg tctgcctcct gcttttgcac gcggccgtgg acgcgacctc
     3841 aatgataatg ataaccccct gaatcaacca atttccagta taaccagcac ccgtttg
//
LOCUS       DBNGSG010000495         6307 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1180857, whole genome shotgun sequence.
ACCESSION   DBNGSG010000495 DBNGSG010000000
VERSION     DBNGSG010000495.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6307)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6307)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6307
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1180857"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="AC158Q_13095"
     CDS             <1..190
                     /locus_tag="AC158Q_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010814699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HIR3340753.1"
                     /translation="LGKIKTVSQMVAIPLLLYHDPIGAFSPQRVGTWLIYLAAVLTLA
                     SMVYYLKTALPLAWRKPS"
     gene            241..316
                     /locus_tag="AC158Q_13100"
     tRNA            241..316
                     /locus_tag="AC158Q_13100"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:274..276,aa:Gly,seq:gcc)
     gene            389..462
                     /locus_tag="AC158Q_13105"
     tRNA            389..462
                     /locus_tag="AC158Q_13105"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Cys,seq:gca)
     gene            537..623
                     /locus_tag="AC158Q_13110"
     tRNA            537..623
                     /locus_tag="AC158Q_13110"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:571..573,aa:Leu,seq:taa)
     gene            831..1286
                     /locus_tag="AC158Q_13115"
     CDS             831..1286
                     /locus_tag="AC158Q_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HIR3340754.1"
                     /translation="MIVFNLGCENSHRFEGWFASGDEFERQIEGKLVTCPLCGSANIS
                     RLPHAAHINKAVSKAPRPSRGESAAATLQQYANVGSEALARLVEHIVENTDDVGAAFP
                     EEARKIHYQEAPERRIRGTASRDEIEALKDEGVEVVALPIPAHRLGKTH"
     gene            1340..1783
                     /locus_tag="AC158Q_13120"
     CDS             1340..1783
                     /locus_tag="AC158Q_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3340755.1"
                     /translation="MFKHILLPTDGSKLSAKAVKRGIELAKKSKARVTVIHIVPEFKL
                     MVDEGITMLSAALKKRFEEEGRARAQKMLDDIARQARARGVRCTTLCVASDLPYLQII
                     SAARKKKCDLILMASHGRRGLSSLLLGSETAKVLLHTKVPVLVVR"
     gene            complement(1832..2929)
                     /locus_tag="AC158Q_13125"
     CDS             complement(1832..2929)
                     /locus_tag="AC158Q_13125"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3340756.1"
                     /translation="MTVSRCARKARSLADSGQPLRNERSRCDKFACDSAQPLKEKTTM
                     RRFITAFGAIILGLTCVTASAQIYPAKPIRLIVPYPPGGPTDLVGRSIGQKLTEAWGQ
                     PVVVENRAGAASAVGTEVASRAPADGYTLLLGTSAGFCINPALGGKMLYDPERDFAPI
                     SLLVINPQILVVHPSLPVRSVKELIALAKTRPGQINYASVGNASPQHLGMEMLKSMTG
                     INMIHVPYKGTAPAITDILAGNVSLMFNSMPSVLPQANAGKLRGIAVGSARRSPAAPE
                     IPTVAEAGVPGFEYVTWYGLFAPAGTPRDIVTKLNARIVKILADQELSRRFSSQGAEP
                     QSNTPEQLAQYRRTEFERWRKLIAELKLKVE"
     gene            2928..3743
                     /locus_tag="AC158Q_13130"
     CDS             2928..3743
                     /locus_tag="AC158Q_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HIR3340757.1"
                     /translation="MNHPAGPIPTLTDKLIARKEGAIGWVIFNNPERHNATSYEMWQS
                     LPLVLEAYVRDPDVRVVVFRGAGEKAFSAGADISQFKEKRTSAEAVKEYNAAADTASR
                     SLRECAKPTIAMIRGYCIGGGTAIAVGCDIRIAGDDARFGVPAAKLGLGYRFEGIKRL
                     ASIVGPSFTAEIFFTARQFSAQEALQMGLVNRLVPAADLESYTLDYANSIAINAPLTI
                     AAVKRALVELNKDPSQRDLGSCQNMVDACFVSEDYREGQTAFMEKRKPQFKGR"
     gene            3785..5314
                     /locus_tag="AC158Q_13135"
     CDS             3785..5314
                     /locus_tag="AC158Q_13135"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - (3S)-3-hydroxyacyl-CoA
                     dehydrogenase (NAD+) activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HIR3340758.1"
                     /translation="MAFDANRADLIVGVIGTGAMGRGIVQVAAAGGMQVLMTDSRAGA
                     AAEAREFVGKMLMRAAEKGATTRDEAVAALDRIKTVSGPADMKPCHVVIEAIVENLQA
                     KQELFAELEAVVAPDCRLVSNTSSLSVTSIAARLKTPQRFAGFHFFNPVPLMKLVEVI
                     DGLQTEEWVGDALMSLGRRMTREPVRLKDAPGFLVNQVGRGFTLEGSHLVYEGIAGFA
                     DVDRVMRDVGGFRMGPFELMDLTGLDVTQPASELIYNQFFQETRYRPNLIMRSRYEAG
                     VLGRKTRRGFYDYDAEMKAAVAAEPAAPAAHSGSVWVSPADSQGHAALTALLGKLGAQ
                     VETGAKPGSQALILVTPVGEDTTTCAVEQGLDPRRTVAVDTLFPMAKRRTLMGTPLTD
                     ETYRRGAHGLLASDGVPVTVCRDSPGFIAQRIVAMIVNIGCSIAQSRTAQPADIDKAV
                     TLGLNYPNGPFKFGDVLGPGRVYQVLSGMYRIYGDPRYRPNIWLTRRARLGVSLLTPE
                     P"
     gene            5354..>6307
                     /locus_tag="AC158Q_13140"
     CDS             5354..>6307
                     /locus_tag="AC158Q_13140"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HIR3340759.1"
                     /translation="MLDAYLYEGLRTPFGRHAGVLAKVRPDDLLADAIKNLMKKSAFK
                     PEQVEDVIVGCANQAGEDSRCVARHAGLVGGLPVETPGTVVQRNCASGLGALVQASHA
                     ITAGEGEVFLVGGVESMSRSPLVMSRSESAFGRDVKLYDSTIGPRFSNPKLVKRFGDD
                     QMPQTADTLAREYEIKRDEADHFALRSQQKYAIAKGEGFFSGEIVAVELPPSRKGPVP
                     PIADDEHPRPNTDLAALATMRPLYEGGVTTAGNASGVNDGAVALILASRTAGDKAGVK
                     PIARVIASAAAGVPPQIMGIGPVAASKKALARAGLTMKDLDI"
BASE COUNT         1203 a         1926 c         2006 g         1172 t
ORIGIN      
        1 tctcggcaag atcaagaccg tttcgcagat ggtggcgata ccgttgctgc tctaccacga
       61 ccctatcggc gccttcagcc ctcaacgggt cgggacctgg ctcatttatc tcgccgcggt
      121 cctgacgctg gcatccatgg tctactatct caagacggcc ctgccgctcg cgtggcgcaa
      181 gccgtcctag ttacggttgc cggggcgggg ccggctgacg tataattttc cttaggaaac
      241 gcgggaatag ctcagttggt agagcgcaac cttgccaagg ttgaggtcgg gagttcgaga
      301 ctcctttccc gctccagaat tcccggggat aacgaaggcc gcttgcgcgg tttgaggctt
      361 cgttatcccc ttttgtttca gtatcagcgg cggggtggca gagtggtcat gcagcggcct
      421 gcaaagccgt gtacgccggt tcgattccga cccccgcctc caaagcttgc aaccgtgcga
      481 accgggatcc agatcgatca cgttgcgcgc gggcaggagt taaaagcttt ttgcccgccc
      541 gggtggcgta attggtagcc gctggggact taaaatcccc tggccgcgag gccgtgccgg
      601 ttcgactccg gccccgggca ccattcgtat ggcccaggac gtcccgggga tggctcaggg
      661 agaatttcca cgctccacaa tctattgcgg cagaggtccc cgcgaaaccc agattttgtg
      721 accgggttac tttgtgagat gatttgcgca ggacttggga gccgggcatg ggattcgctg
      781 tcacacaagt tggccgctgc aatcggccat gactgcaatc aggggacgat gtgatcgttt
      841 ttaatctggg atgcgagaac agtcaccggt tcgagggctg gtttgcctcg ggcgatgaat
      901 tcgagcgtca aatcgagggc aagcttgtta cctgcccatt gtgcggtagt gccaatatct
      961 cacgcctgcc gcatgccgca catatcaaca aggccgtgtc gaaagcaccc cgcccgtcga
     1021 ggggcgagtc tgcggccgcc acgttgcagc aatatgccaa tgtaggcagc gaggcgctcg
     1081 ccaggctggt cgagcacatt gtcgagaaca cggatgacgt cggcgccgcc ttccccgagg
     1141 aggcgcgcaa gatacattac caggaagcgc ccgaacgccg gatccgcggc actgcgtcgc
     1201 gcgacgagat cgaagcgctg aaggacgagg gagtcgaggt cgtcgcgctg cccattcctg
     1261 cccatcgcct gggcaagacg cactgaacgc ggataaattt cgctccggtt ttttaattcc
     1321 gggtcgacaa ggagacgcca tgttcaagca catactgcta cccaccgacg gatcgaaact
     1381 ttccgccaag gcggtcaagc gtggcatcga gcttgcgaag aagagcaagg cgcgcgtgac
     1441 ggtcatccac atagtgcccg agttcaagct catggtcgat gaaggcatca ccatgctcag
     1501 cgccgccctc aagaaacgct ttgaggaaga ggggcgcgcg cgggcacaga agatgctcga
     1561 cgatattgct cggcaggcgc gcgcccgcgg cgtgcgctgc acgactcttt gcgtagcgag
     1621 cgaccttcct tatctgcaga ttatttccgc ggcacggaaa aagaagtgtg acctcatcct
     1681 gatggcatcc cacggccgcc gcgggttgtc cagcctgttg ctcggcagcg agactgccaa
     1741 ggtactgctg cacaccaagg tgccggtgct ggtcgtgcgc tgacacggcg ccgccgcttt
     1801 tccctcgtgg aaagagcggt gttcccgaag gtcactcgac cttcagcttc agttcggcaa
     1861 tcagcttgcg ccagcgctcg aattccgttc ggcggtactg cgccagttgt tcgggagtat
     1921 tgctttgggg ttccgcgccc tggctcgaga aacgcctgga gagttcctga tcggcgagga
     1981 tcttgacgat ccgggcattc aacttggtca caatatcacg tggagtaccc gcgggcgcaa
     2041 acaagccgta ccacgttacg tattcgaagc cgggaacccc ggcttcagct actgtcggta
     2101 tttccggagc ggctggtgat cgccgggcgc tgcctaccgc aatgcctctc agcttgccgg
     2161 cattcgcctg ggggagcacc gagggcatgc tgttgaacat cagcgacacg ttgccggcga
     2221 gaatgtcggt gatcgcaggc gcggttccct tgtaaggaac atgaatcatg ttgatgccgg
     2281 tcatgctctt cagcatttcc ataccgagat gctgcgggct ggcattgccc accgacgcgt
     2341 aattgatttg cccgggtcgg gttttcgcga gcgcgatcag ttccttcact gaacgtaccg
     2401 gcagcgacgg atgcaccacc aggatctgcg ggttgatgac gagcaggctg atcggcgcga
     2461 aatcgcgttc cggatcgtag agcattttcc cgcctaatgc cgggttgata cagaagccgg
     2521 ccgaagtccc gagaagaagg gtgtagccgt ccgcgggggc ccgcgaagcg acctcggtac
     2581 cgaccgcgct ggcagcgccc gcgcgatttt ccaccacgac cggctgtccc caggcttcgg
     2641 tcagcttttg cccgatcgag cggcccacga gatccgtggg gccgccgggc ggatagggca
     2701 cgatcaaccg gatcggtttg gccgggtaga tctgggccga tgcggtgaca caggtcagtc
     2761 ccagaatgat cgcgccaaac gcggtgatga atcgccgcat agtcgtcttc tccttgagag
     2821 gttgtgcgga atcgcatgca aatttatcac aacgcgatct ttcgttcctc agcggctgcc
     2881 ccgaatcggc taaactacgg gccttgcgag cacaccggga caccgtcatg aatcatccag
     2941 caggaccgat acccactctc acggacaagc tgatcgccag gaaggaaggg gctatcggct
     3001 gggtcatctt caacaacccc gaacgccaca atgcgacttc gtatgaaatg tggcaatcgc
     3061 tgccgctcgt gctggaggct tatgtccgcg accccgacgt gcgtgtcgtc gtgttccggg
     3121 gtgcagggga gaaggcattc agcgccggcg ccgacatttc ccagttcaag gaaaaacgca
     3181 ccagtgctga ggcggtgaag gaatacaatg ccgctgcgga tacggcgagc cgctcgctgc
     3241 gggaatgcgc caagcctacg atcgcaatga tccgcggcta ttgcatcggc gggggcaccg
     3301 ccatcgctgt gggatgcgac atccgcatag ccggggacga tgcacgcttc ggcgtgccgg
     3361 cggccaagct tggcctgggc taccggtttg agggcatcaa gcgcctggcc agcatagtcg
     3421 gcccatcgtt tacagcggag atcttcttca ccgcgcgcca gttcagcgcg caggaggcgc
     3481 tgcagatggg gctcgtgaat cgcctcgtgc ccgccgcgga cctcgaaagc tacacgctcg
     3541 actatgccaa ttccattgcc atcaacgcgc ccttgaccat tgcggcagtc aagcgcgcgc
     3601 tggtcgagct caacaaggat ccctcgcagc gcgatctcgg gtcttgccag aacatggtgg
     3661 atgcctgctt cgtgagcgag gattacaggg aggggcagac ggccttcatg gagaaacgca
     3721 aaccccaatt caagggccgc tgaaggcgtt ggcttcaaga caatctacgg gagcaggtta
     3781 aatcatggca tttgacgcaa accgcgccga cctcatcgtg ggcgtgatcg gtactggcgc
     3841 catgggccgg ggcatcgtgc aggtcgccgc ggcgggcggc atgcaggtct tgatgacgga
     3901 ctcccgcgcc ggggcagctg cagaagcacg cgagttcgtg ggcaagatgc tcatgcgtgc
     3961 ggcggagaag ggcgccacca cccgcgatga ggcggttgcg gccctcgacc gcatcaagac
     4021 cgtttccggg cccgcggata tgaagccgtg tcatgtggtg attgaagcca tcgtcgaaaa
     4081 tctgcaggcc aagcaggaac tcttcgccga actggaggcg gtggttgcgc ccgactgcag
     4141 actcgtctcc aatacttcct cgctttccgt cacatcgatc gcggccaggc tgaaaacgcc
     4201 tcagcgcttc gccggctttc atttctttaa cccggtaccg ctgatgaaac tggtggaagt
     4261 gatagacggc ctccagaccg aggaatgggt cggcgacgcg ctgatgtcgc tgggccgccg
     4321 catgacgcgc gagccggttc gattgaagga tgcgcccggc ttcctggtca accaggtggg
     4381 acgcggtttc acgctcgaag ggtcgcacct cgtgtatgag ggcatagcgg gatttgccga
     4441 cgtagaccgc gtgatgcgcg acgtcggagg attccgcatg ggaccattcg aactgatgga
     4501 tctgaccggg ctcgacgtga cccagcccgc ttccgagctc atctacaacc agttcttcca
     4561 ggagacgcgc taccgtccca atctcatcat gcgcagccgt tacgaggcgg gggtgctggg
     4621 ccgcaagacc aggcgcggtt tctacgacta cgacgcggag atgaaggctg ctgttgcggc
     4681 ggagccggcc gcgcccgccg cgcactccgg cagcgtctgg gtgagcccgg ccgactccca
     4741 ggggcatgcc gcgctcaccg cgttgctcgg aaagctgggg gcgcaagttg aaaccggcgc
     4801 caagcccggc tcacaagccc tgattctggt gacgccagtc ggcgaggata cgaccacgtg
     4861 cgcggtggag caggggctcg accccaggcg cacggtggcg gtggacaccc tgttcccgat
     4921 ggccaagcgc cgcaccctga tgggcacgcc gctgactgac gagacctatc gccgcggcgc
     4981 gcacgggctg ctcgcctcgg acggtgtacc ggtgacggtc tgccgggaca gcccgggttt
     5041 catcgcgcaa cgtatcgttg ccatgatagt caacataggc tgttccattg cccagagccg
     5101 caccgcccag cctgccgata tcgacaaggc cgtgacactc ggcctcaact acccgaatgg
     5161 accattcaaa ttcggtgacg tgctcggtcc tggacgcgtc taccaggtat tgtcgggcat
     5221 gtatcgtatt tacggagacc cacgctaccg ccccaatatc tggctcacac gacgcgcccg
     5281 gctgggcgtt tcgctgctga ccccggagcc ctgatgaccg cacctatcac gatgacccga
     5341 gaaggagatc gccatgcttg atgcctatct ttacgaaggc ctgcgcaccc cgttcggccg
     5401 ccatgccggc gtgctcgcca aagtacgtcc cgacgacctg ctcgccgacg ccatcaagaa
     5461 cttgatgaag aaatcggcgt tcaagccgga gcaagtcgag gacgttatcg tggggtgcgc
     5521 caaccaggcg ggcgaggaca gtcgctgcgt ggcgcggcac gcgggactcg ttggaggttt
     5581 gccggtcgag acgccgggca ccgtggtcca gcgcaactgc gcgagcggtc tgggcgccct
     5641 ggtgcaggcg tcacacgcca tcacggccgg cgaaggagag gtattcctgg tcggaggcgt
     5701 ggaaagcatg agccgctccc cgctcgtcat gagccggtcg gaatcggcgt ttggtcgtga
     5761 cgtcaagctc tacgacagca cgatcggccc gcgattttcg aatcccaagc tggtgaagcg
     5821 cttcggcgat gaccaaatgc cgcagaccgc cgataccctc gcgcgtgaat acgagatcaa
     5881 gcgcgacgag gcggaccatt tcgcgctgcg ctcgcagcag aagtacgcga tcgccaaggg
     5941 cgagggcttc ttttccggag agatcgtggc ggtagagctg cctccatccc gcaagggccc
     6001 ggtgccgccg attgcagatg acgagcatcc gcgacccaat accgatctcg cggcccttgc
     6061 cacgatgaga ccgttgtacg aaggcggcgt gaccacggcc ggcaatgcct caggcgtcaa
     6121 cgacggagcg gttgcgctga ttctcgcttc caggacggcc ggcgataaag cgggcgtcaa
     6181 accgatcgcg cgcgtgatcg cgagtgcggc ggctggcgtc ccgccccaga tcatgggtat
     6241 cggcccggtc gcggcttcca aaaaggcgct ggcgcgtgcc gggctcacga tgaaggacct
     6301 cgatatc
//
LOCUS       DBNGSG010000496         3747 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_4747873, whole genome shotgun sequence.
ACCESSION   DBNGSG010000496 DBNGSG010000000
VERSION     DBNGSG010000496.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3747)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_4747873"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="AC158Q_13145"
     CDS             <1..763
                     /locus_tag="AC158Q_13145"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HIR3340760.1"
                     /translation="TGAGGIIGADAVAKSTPDGYTFLMGPTTTAINASLVVKPPYNMR
                     RDLQPVAMLSFYPMAAGVLADSPVRSLKDLVALSHKQSLSAGSGGNGTPQHLVLEMFK
                     GATGAKILHVPYKGGNPALVALLGGQNDVVFSLWPEALPHVQAGKLRALAVTTPARLA
                     QMPDLPTTAEAGFPGVQATGWQGVMVRAGTPKDIVARINAQVGSIMAVPDMKSRIVEQ
                     GFIPVTMGVGDTEKFVNADVERWGKIIRDGNIKSE"
     gene            complement(961..1860)
                     /locus_tag="AC158Q_13150"
     CDS             complement(961..1860)
                     /locus_tag="AC158Q_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HIR3340761.1"
                     /translation="MDKFRAMAAFVQIVEKGSLTAAADALGASLPSTVRTLAALERSL
                     GARLLNRTTRRIALTDEGRAYYERARRILGDVADAEAAVSMKQGRPRGRLAIGSSVLF
                     GRLHLAPLVSEFVARHPEVKVELLMLDRVVNLVEEGLDASVRIGVLADSSLVAIGIGR
                     TRRVVCASPAFVKRHGMVRAPAELARHPCIRFTGLASGDWEFRAGGKPVKVRVEGPVA
                     TNQVDAAIDACVQGLGYGMFFCYQVNAHVRAGRLRYVLEAHEPEPVPVSLVYPQARLV
                     SARLRAFIDWVVPRMRERLGAAK"
     gene            1982..2530
                     /locus_tag="AC158Q_13155"
     CDS             1982..2530
                     /locus_tag="AC158Q_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3340762.1"
                     /translation="MSRIQPVDPRAASGEAKQLLDAVQSQLGATPNFIRVLARSPKAL
                     GGFLGLYGGVGGSSIDLATRERIALAVAESNACSYCVAAHTAIGRGAGLSSEEMHRNR
                     EGGSADARAAAAVAFARALNEHRGEVTAVEFDAVRRAGYDDAQIVEIIAIVALNVFTN
                     LLGKSVQVDIDFPKVPLLKAAA"
     gene            2556..3110
                     /locus_tag="AC158Q_13160"
     CDS             2556..3110
                     /locus_tag="AC158Q_13160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR3340763.1"
                     /translation="MAHRFSEILFTEGVRAAQERYGSRPQNERLRALGPANDTLGEDE
                     RGFIEARDGFYLASVSESGWPYLQFRGGPAGFVRVLDDRTLGYAEFRGNRQYVSTGNI
                     DRDGRVALFFMDYASRRRLKLLGRASILDAAQSPELAKRLELPGYRARVERAVLIGVE
                     AFDWNCPQHITPRYTEAEFALLKG"
     gene            3236..3502
                     /locus_tag="AC158Q_13165"
     CDS             3236..3502
                     /locus_tag="AC158Q_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HIR3340764.1"
                     /translation="MTKSRSLLSVRLDPALSEALDRHCEATGATRSHVVQQGIAQYLV
                     AQTGPTLGSLAEAVLSAIPKQAARGARAPRQRRFRDYVREKRRR"
     gene            3483..>3747
                     /locus_tag="AC158Q_13170"
     CDS             3483..>3747
                     /locus_tag="AC158Q_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HIR3340765.1"
                     /translation="MKSVVVDSGFLIGLFDETDPLHARCRGFLRDYRGRFLTTEAVLT
                     EALALLSQAQQLRCLDWLGDAARAGLLAVDREPVDFRAVEKFAR"
BASE COUNT          599 a         1315 c         1230 g          603 t
ORIGIN      
        1 aacgggcgcc ggcggcatca ttggcgccga cgcggtcgcc aaatcgactc ccgatggata
       61 caccttcctg atgggcccga cgaccaccgc gatcaacgcg agcctggtcg taaagccgcc
      121 ttacaacatg cggcgggatc tgcagcccgt cgccatgttg agcttctatc ccatggctgc
      181 cggggtgctg gccgactcgc cggtccgctc gttgaaggat ctcgtcgcgc tatcgcacaa
      241 gcaatcgttg agtgcgggct cgggcggcaa tggaacgccc cagcatctcg tgctcgaaat
      301 gttcaagggc gcgaccggcg caaagatcct gcacgtgccc tataaaggcg gcaacccggc
      361 gctggtggct ttgctgggcg gccagaacga cgtggtgttt tcgctgtggc cggaagccct
      421 tccccacgtg caggcgggca agcttcgcgc gctcgccgtg acgacccctg cccggcttgc
      481 gcagatgccc gatctgccaa ccacggcgga agccggattc cccggcgtcc aggccacggg
      541 ctggcaaggc gtgatggttc gcgccggcac gccgaaagac attgttgcca ggatcaatgc
      601 gcaggtgggc agcatcatgg ccgtgcccga catgaaatcc aggatcgtgg agcaaggctt
      661 catcccggtc acgatggggg tcggcgatac cgagaaattc gtcaacgccg acgtcgagcg
      721 ctggggaaaa atcatccgcg acgggaatat caagtcggag tagtgattcg gtgaagagcc
      781 gaaagaacgt ctcaggtact gcgtcgcaga tactgcgtct caggtactgc gtctcagata
      841 cggcgacgtt ttgtttggaa cgcctcatct gagacgattc atctgagacg cctcatctga
      901 gaagcctcat ctgggacgct tttcagctct cccgccttcc gcctttcgac tttcgccttc
      961 ttatttcgcc gcccccagcc tctccctcat cctcggcacg acccagtcga tgaacgcccg
     1021 caggcgcgcg gagacgagcc gcgcctgggg atagacgagc gacacgggga ccggctcggg
     1081 ctcgtgggcc tcgagcacgt agcgcaggcg gccggcgcgc acgtgcgcgt tcacctggta
     1141 gcagaagaac atcccgtagc ccagcccctg cacgcaggcg tcgatcgccg cgtcgacctg
     1201 gttggtcgcg accggcccct ccacgcgcac cttcaccggc ttgccgccag cccggaattc
     1261 ccagtcgcct gacgcgaggc cggtgaagcg gatgcaggga tggcgcgcga gctcggcggg
     1321 ggcccggacc atgccgtggc gcttcacgaa cgccgggctc gcgcacacca cgcggcgcgt
     1381 gcggccgatc ccgatcgcca ccagcgacga gtcggccagc acgccgatgc gcaccgacgc
     1441 gtcgagaccc tcctcgacca ggttcaccac gcggtcgagc atcagcagct cgaccttcac
     1501 ctcgggatgg cgcgcgacga actcggagac gagcggcgcg aggtgcagcc gcccgaacag
     1561 caccgaggag ccgatcgcga gccggccgcg cggcctgccc tgcttcatcg acaccgccgc
     1621 ctccgcgtcg gcgacgtcgc ccaggatgcg gcgcgcgcgc tcgtaatagg cacgcccctc
     1681 gtcggtcagc gcgatgcggc gggtggtgcg gttcagcagc cgcgcgccga gcgagcgctc
     1741 aagcgcggcg agcgtcctca cggtcgaggg cagcgaggcg ccgagcgcgt ccgcggcggc
     1801 ggtgaggctg cccttctcga cgatctgcac gaacgccgcc atggcgcgga acttgtccat
     1861 gggctgatta ttccactaaa tgcagtaatc aagtgttatt cgcgccattt atccattccc
     1921 ggccggcgcc cagaatccac tccgctgcca atcgggcaga tccaaaccca gggagatcac
     1981 cgtgtcacgc atccagccgg tagacccccg cgccgccagc ggggaagcga agcaactgct
     2041 cgacgccgtc cagtcccagc tcggcgccac ccccaatttc atccgcgtgc tcgcccgctc
     2101 gcccaaggcg ctcggcggtt tcctcggcct ctacggcggc gtcggcggct cgtccatcga
     2161 cctcgccacg cgcgagcgca tcgcgctggc cgtcgcggag agcaacgcat gctcctactg
     2221 cgtcgccgca cacaccgcca tcggccgcgg cgccggcctc tccagcgagg agatgcaccg
     2281 caaccgcgag ggcggctcgg cggacgcgag ggcggcggcc gcagtggcct tcgcccgcgc
     2341 gctgaacgag caccgcggag aggtgacggc cgtggagttc gacgcggtgc gccgcgccgg
     2401 ctacgacgac gcgcagatcg tcgagatcat cgcgatcgtg gcgctgaacg tgttcaccaa
     2461 cctgctcggg aaatcggtcc aggtggacat cgattttccg aaagtcccgc tgctgaaggc
     2521 cgcggcctga accgccggca ttcccggaga aaatcatggc acacagattc tcggagatcc
     2581 tgtttaccga aggcgtgcgc gcggcgcagg agcgctacgg cagccgcccg cagaacgagc
     2641 ggctgcgcgc gctggggccc gcgaacgaca cactgggcga ggacgagcgc gggttcatcg
     2701 aggcgcggga cggcttctac ctcgcgtcag tgagcgagtc gggctggccg tacctccagt
     2761 tccgcggcgg gccggcgggc ttcgtgcgcg tgctcgacga ccgcaccctg ggctatgccg
     2821 agttccgcgg caaccgccag tacgtgagca ccggcaacat cgaccgcgac gggcgcgtcg
     2881 cgctgttctt catggactac gccagccgcc gccggctcaa gctgctcggc cgcgccagca
     2941 tcctcgatgc ggcgcagtcg cccgagctgg cgaagcgcct cgagctgccg ggctaccgcg
     3001 cccgcgtcga gcgcgcggtg ctgatcggcg tggaggcatt cgactggaac tgcccccagc
     3061 acatcacgcc gcgctacacc gaggcggagt tcgccttgct gaaggggtga ctcgtttccc
     3121 gtttctcgtt tctcgtttct cgtttctcga tccctgcgct gcggaaggcc cgttactcgt
     3181 caggagccgg gctttcgcct tgctccttcc gttgtcatac agtatagtag tttgtatgac
     3241 aaaatcccgc tccctgctct cggtccggct cgacccggcg ctctccgagg cgctcgatcg
     3301 ccactgcgag gcgacgggcg cgacccgcag ccacgtcgtg cagcagggca tcgcccagta
     3361 cctggtcgcc cagaccgggc cgaccctcgg cagcctcgcc gaggcggtcc tgtcggccat
     3421 cccgaagcag gcggcgcgcg gcgcgcgcgc gccccgtcaa cgccgtttcc gcgattacgt
     3481 tcgtgaaaag cgtcgtcgtt gactcgggtt tcctgatcgg gctgttcgac gagacggatc
     3541 ccctccacgc ccgctgccgc gggttcctgc gcgattaccg cggccgcttc ctcacgaccg
     3601 aggccgtgct gacggaggcg ctggcgttgc tgtcgcaggc gcagcagttg cgctgcctgg
     3661 actggctggg cgacgccgcg cgggccggct tgctggctgt cgatcgcgag cccgtggatt
     3721 tccgggccgt cgagaagttc gcgcgca
//
LOCUS       DBNGSG010000497         3752 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2540322, whole genome shotgun sequence.
ACCESSION   DBNGSG010000497 DBNGSG010000000
VERSION     DBNGSG010000497.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2540322"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            264..1280
                     /locus_tag="AC158Q_13175"
     CDS             264..1280
                     /locus_tag="AC158Q_13175"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007744784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR3340766.1"
                     /translation="MPDDVRDLSNPVSTSGSFTDDGLLDAYSRAVTGVAEMVGPAVVR
                     LDIKRRMRVRDARGREVAQEAGGSGSGFIFTPDGLIATNSHVVSGATAIGVTLNDGQV
                     LQADLIGDDPDTDLAVVRVAGHKLPSVALGESRGLRVGQLAVAIGNPYGFQCSVTAGV
                     VSALGRSLRSQNGRLMDDVIQTDAALNPGNSGGPLVSSRGEVIGVNTAVILPAQGICF
                     ATAIDTAKVTLAQLLKHGRVRRARLGLAGQNIVLPRRMVRHYGLERDGAVQAASIERG
                     GPASRAGLEQGDIIVGFAGQPVAGFDDLHRLLTEEKLGGRVPVTVLRRGRQLTLEIQP
                     DESR"
     gene            1420..2283
                     /locus_tag="AC158Q_13180"
     CDS             1420..2283
                     /locus_tag="AC158Q_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HIR3340767.1"
                     /translation="MKKIVSIHRGPPAHWVGDGFHVRSLFTYDGRARDLSPFLLLDYA
                     APEKFAPSDAMRGVGQHPHRGFETVTIAYQGGVEHRDSAGNHGIIGPGDVQWMTAASG
                     IVHEEWHEREFTRRGGTMEMAQLWINLPAKDKMAAPGYQDIRGDRIPSVSLGSGSSVR
                     VIAGTFRGTRGPARTFTPINVWDARIQAGDRIELDFPAGYTTLIAVLKGRAAVNDGET
                     ASAAELVILERAGTRVAITAEADATVLVLNGEPIDEPVVGYGPVVMNTRAEIQQAFRD
                     FHSGRLGQAGA"
     gene            2315..3022
                     /locus_tag="AC158Q_13185"
     CDS             2315..3022
                     /locus_tag="AC158Q_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HIR3340768.1"
                     /translation="MLTVRKAQERGHANHGWLDSWHSFSFADYQDPAHMGYGPLRVIN
                     EDRVQAGSGFGTHGHRDMEIISYVLDGALGHKDSMGNGSSIVPGDVQRMSAGTGVRHS
                     EYNHSKDGTTHFLQIWIEPKITGIRPSYEQKHFPAAEKRGRLRLIASPDGREGSVSMN
                     QDAFLYAGLFDGAERAGLPLAAGRRGYVHIARGAIEVNGRRLDAGDAMKLDGGTTIEL
                     ERGQGAEVLVFDLPWKA"
     gene            3073..3495
                     /locus_tag="AC158Q_13190"
     CDS             3073..3495
                     /locus_tag="AC158Q_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR3340769.1"
                     /translation="MVNQNTAPYAALVLRLTLGVMFIAHALLKVVVFTVPGTVQFFES
                     VGLPGVLAYIVIAAELGAGVLLIAGVATRWIALATIPILLGAAWVHLPNGWVFSAQGG
                     GWEYPVFLAIAAFVQFLLGDGAYALASRPAPLATGRAA"
BASE COUNT          669 a         1181 c         1248 g          654 t
ORIGIN      
        1 gcggcatgtc gatcggcacg ctgttcacgc tcttcgtcat tcccgtcgcc tatacctaca
       61 tcgtcggcga gcgccagctt gcggcgggcg aagagggcgg cggcggggcg gttgcgcatg
      121 cgcccgcaaa caaggcataa ggcacaaggc acaagacagt aagacgttgc cgaagaggtt
      181 ttcccttttg ccttttgtct tgtgactttt gccctaagta ggcggcggtt gattcgccgc
      241 ccgcccgccc ccatttccct tggatgccgg atgatgtccg ggacctttcc aatcccgtaa
      301 gtacttcagg gtctttcacc gacgacggcc tgctcgacgc ctattcgcgc gccgtcaccg
      361 gagtggccga gatggtcggc cccgccgtgg tgcggctcga catcaagcgc cgcatgcgcg
      421 tccgcgacgc gcgcggccgc gaggtcgcgc aggaagccgg cggcagcggc tcgggattca
      481 tctttacgcc cgacggcctg atcgcgacca acagtcacgt cgtgtccggc gccaccgcga
      541 tcggcgtgac gctgaacgac ggccaggtgc tgcaggccga cctgatcggc gatgatcccg
      601 atacggacct cgcggtggtg cgtgtcgcag ggcataagct gccgtcggtc gcgctcggcg
      661 aatcgcgcgg cctgcgggtg gggcagctcg cggtcgcgat cggcaatccc tacggcttcc
      721 agtgcagcgt caccgcgggc gtcgtgtccg cgctcggccg ctcgctccgg tctcagaatg
      781 ggcgcttgat ggacgacgtg atccagaccg acgccgcgtt gaacccgggc aattcgggcg
      841 gaccgctggt gtcctcgcgc ggcgaggtga tcggcgtcaa taccgcggtc atcctgcccg
      901 cgcagggcat ttgcttcgcc accgcgatcg acacggcgaa ggtgacactc gcgcaattgc
      961 tcaagcatgg gcgcgtgcgg cgcgcgcggc tggggctcgc ggggcagaac atcgtgctgc
     1021 cgcggcgcat ggtgcggcac tacgggctgg agcgcgacgg ggcggtgcag gccgcctcga
     1081 tcgagcgcgg cgggcccgcg agccgcgccg ggctcgagca gggcgacatc atcgtcggtt
     1141 tcgccggcca gccggtcgcc ggcttcgatg acctgcaccg gctgctgacc gaggagaagc
     1201 tcggcggccg cgtgccggtt accgtgctgc gccgggggcg gcagctgacc ctggaaattc
     1261 aacccgacga atccaggtaa acaaaatcgt cagatgacaa taatatattg accaataacg
     1321 agtttcttat tcagaataac tgaccaagtt agtcatatta ttctgattac attccttttg
     1381 ccgcgcacct atattgttgg catgaaatcg gaaaacgcca tgaagaagat cgtcagcatt
     1441 caccgcggcc cgccggccca ctgggtcggc gacggcttcc acgtgcgcag cctgttcacc
     1501 tacgacgggc gggcccggga cctgagtccg ttcctgctgc tcgattacgc ggcgcccgag
     1561 aagttcgcgc cctcggatgc aatgcgcggc gtcggccagc acccgcaccg cggcttcgag
     1621 accgtcacca tcgcctacca gggcggggtg gagcaccggg attcagccgg caaccacggc
     1681 atcatcggcc cgggcgacgt gcaatggatg acggcggcct cgggcatcgt gcacgaagag
     1741 tggcacgagc gcgagtttac ccggcgcggc ggcacgatgg agatggccca gctctggatc
     1801 aacctgccgg cgaaggacaa gatggccgcg cccggctacc aggacattcg cggtgatcgg
     1861 ataccctccg tcagcctggg atccggttcg agcgtgcgcg tgattgccgg gacgttccga
     1921 ggcacccgtg gcccggcgcg caccttcacg ccgatcaacg tgtgggacgc gcggatccag
     1981 gccggcgacc gcatcgagct ggattttccg gcgggataca ccacgctgat cgccgtgctg
     2041 aaaggcaggg ccgcggtgaa tgacggagag accgccagtg ccgcggagct ggtgatcctg
     2101 gagcgcgcgg gcacgcgggt tgcgatcacg gctgaagcgg acgcgacggt acttgtgctg
     2161 aacggcgagc cgatcgacga gccggtggtc ggctacggcc cggtcgtcat gaacacgcgc
     2221 gcggagatac agcaggcgtt tcgcgatttt cacagcggcc ggctgggaca ggcgggcgcc
     2281 tgaaccgccg ccccggacgc gacaaggagt caatatgctc acggtcagaa aagcccagga
     2341 gcgcggtcat gccaaccacg gctggctcga ttcctggcac agtttttcgt tcgcggacta
     2401 ccaggacccg gcacacatgg gctacgggcc gctgcgcgtg atcaacgagg accgcgtcca
     2461 ggcgggctcg ggcttcggca cgcacggcca ccgcgacatg gagatcatca gctacgtgct
     2521 cgacggcgcg ctgggacaca aggactcgat gggcaacggc tcgtcgatcg tgccgggcga
     2581 cgtgcagcgc atgagcgcgg gcacgggcgt gcggcactcc gagtacaacc acagcaagga
     2641 cgggacgacg cacttcctgc agatctggat cgagccgaaa atcaccggca tccgcccgtc
     2701 ttacgagcag aagcattttc cggctgcgga aaagcgcggg cggctgcgcc tgatcgcctc
     2761 gcccgacggg cgcgaaggct ccgtgagcat gaaccaggac gcattcctgt atgcgggtct
     2821 tttcgacggg gcagagcgcg cgggcctgcc gctcgcggcc gggcgcaggg gctatgtcca
     2881 catcgcgcgc ggcgcgatcg aggtcaatgg ccggcggctc gacgccggcg acgcgatgaa
     2941 gctcgacggc ggcacgacga tcgaactgga gcgcgggcag ggcgccgaag tgctggtgtt
     3001 cgatcttccc tggaaagcgt gattcgtgat tcgtgaatcg taaatcgtca atcaagtatg
     3061 aaaggtgtaa acatggtcaa tcaaaacacg gcgccgtacg cggcgctcgt cctgcggctc
     3121 acgctgggag tgatgtttat cgcccacgcg ctgctgaaag tcgtggtctt caccgttccg
     3181 ggcacggtcc agtttttcga atcagtcgga ttgccgggcg ttctcgccta tatcgtgatc
     3241 gcagcggaac tcggcgcggg cgtgctgttg atcgccggcg tggcaacgcg ctggatcgcg
     3301 ctcgcaacga tcccgatcct gctgggcgcc gcctgggtgc acctgccgaa cggctgggtg
     3361 ttcagcgccc agggcggagg ctgggagtat ccggtgtttc tggctattgc ggccttcgtg
     3421 cagtttctgc tgggcgacgg cgcctatgcg ctcgcgtccc ggcccgcacc gctggccacc
     3481 ggccgcgcgg cgtgactacc cggtaaatgg acaggattaa catttaagca tccaacaccc
     3541 gccaacaccg tcaatagaca ggattaacaa gattaacagg attcaaacta caaccatcta
     3601 ttgctttgtg tgccttaatc ttgtaaatcc tgttaaggaa cctctgcata agtcatcccc
     3661 gcgcaggcgg ggatccagta acgatttgtt taccctggat tcccgccttc gcgggaatga
     3721 cgaaaaacga cttttgcaga gcatccttaa tc
//
LOCUS       DBNGSG010000498         2472 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3898037, whole genome shotgun sequence.
ACCESSION   DBNGSG010000498 DBNGSG010000000
VERSION     DBNGSG010000498.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3898037"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="AC158Q_13195"
     CDS             <1..417
                     /locus_tag="AC158Q_13195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3340770.1"
                     /translation="EGALEEAVRYMRDRSAFGKKIGDFQGMRWKVADMYVEIEAARGL
                     LYRAAVSGDKFPDPTLAAMAKIYTNEMSIRVTSEAIQVHGGYGFTDEFPVSRYFRGTR
                     YGSLGGGTTETLRNLVGKKLVEQMDLATGVLGMGAF"
     gene            complement(538..1584)
                     /locus_tag="AC158Q_13200"
     CDS             complement(538..1584)
                     /locus_tag="AC158Q_13200"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR3340771.1"
                     /translation="MMDAPRHGWRCAGVLAVGMLACAALGFAAPLGTQDKPAAAPPAA
                     QAPSELTIDALSVVKVRSKAVANARSGRSLGPQREGTGVVIDSEGLVLTIGYLIVEAE
                     TVELSTADGRSIPATVVAYDYATGFGLLKALRPLPVRPVQFGQSASIGERELVLIVGF
                     DGVAPAYVVSRRPFIGYWEYLLDEAIYTAPATVNWSGAALLNREGKLLGIGSLVVGDA
                     MGTRTQVPGNMFVPIDLLKPMLGDLIATGKSSARPRPWIGINTQEVQGNVIVTRVSPE
                     GPAESASIRMGDVIVGIGGQPIKGQADFYTRMWKTGEAGVDIPLDVLRGGRVENVTVK
                     SIDRERYYRPKPTY"
     gene            1720..2397
                     /locus_tag="AC158Q_13205"
     CDS             1720..2397
                     /locus_tag="AC158Q_13205"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3340772.1"
                     /translation="MELGLKGKVAVITGATEGIGRATALKLAREGARVAICARRQEPL
                     DTTAAEIGKTGADVLAVSADMSKAADIERFMKAVIDRFGRIDILVNNAGTSARGKFLE
                     IEDKTWSSDIELKVFGAIRCTRLAVPHMKKNGGGRIVNITISSAKQPGAESYPTSVSR
                     AAGLALTKALSKEYAPDNIRVNTVCIGKIKSGQHERRYTRERKSADAYYKEVSRDIPL
                     GRVGEAE"
BASE COUNT          459 a          811 c          807 g          395 t
ORIGIN      
        1 gagggcgcgc tggaggaagc cgtgcgctac atgcgcgacc gctccgcctt cggaaagaag
       61 atcggcgact tccagggcat gcgctggaag gtcgccgaca tgtacgtcga gatcgaggcg
      121 gcgcgcgggc tgctctaccg cgcggccgtc agcggcgaca agttccccga ccccacgctc
      181 gcggcgatgg ccaagatcta caccaacgag atgagtatcc gggtgacgtc agaggcgatc
      241 caggtgcacg gcggctacgg cttcaccgac gaatttcccg tttcgcgtta tttccgcggc
      301 acccgctacg gcagtctcgg cggcggcacc accgagacgc tgcgcaacct cgtcggcaaa
      361 aagctggtcg agcagatgga cctcgccacc ggcgtgctcg gcatgggcgc cttctgacag
      421 gcaagcgccc gtccggagtg ttgagttcta agtgttgagt tttgagttgg tcaacgcgcg
      481 catcgcgtgg cgcagcagat ttcactcaac actcaacact caaaacttaa cactgtttca
      541 ataagtgggc ttgggcctgt aatagcgttc gcggtcgatg gacttgaccg tgacattctc
      601 cacgcgaccg ccgcgcagca cgtcgagcgg gatatcgacg cccgcctccc cggtcttcca
      661 catccgggta tagaaatcag cctggccctt gatgggctgg ccgccgatgc cgacgatcac
      721 gtcgcccatg cgaatactgg cgctttccgc ggggccttcg ggcgagaccc gggtgacgat
      781 tacgttgccc tggacctcct gcgtgttgat cccgatccat gggcgcgggc gcgccgagga
      841 cttgccggtg gcgatgaggt cgcccagcat gggcttcagc aggtcgatcg gcacgaacat
      901 gttgccgggg acctgcgtgc gcgtgcccat cgcgtcgccg accaccagcg acccgatgcc
      961 gagcagcttg ccttcgcgat tgagcagcgc ggcgccgctc cagttcaccg tggccggcgc
     1021 ggtgtagatc gcctcgtcca gcagatattc ccagtagccg atgaagggcc ggcgcgacac
     1081 cacgtaggcc ggcgccacgc catcgaagcc gacgatcagc accagctcgc gctcgcctat
     1141 gctggctgat tgcccgaact gcaccggcct gaccggcagc gggcgcaatg ccttcagcag
     1201 gccgaagccc gtggcgtagt cgtaggcgac gacggtggcc gggatcgagc ggccgtcggc
     1261 ggtggacagc tcgaccgtct cggcttcgac gatcagataa ccaatggtga gaaccaggcc
     1321 ctcggaatcg atcacgacgc cggtgccctc gcgttgcggt cccagcgaac ggcccgagcg
     1381 cgcgttagcg actgccttgc tcctcacctt gacgaccgac agcgcgtcga tggtgagctc
     1441 cgagggggct tgcgcggccg ggggagccgc cgcgggcttg tcctgtgtgc cgaggggcgc
     1501 tgcgaacccg agcgctgcac acgcgagcat gcccacggca agcacacccg cgcaccgcca
     1561 accgtgccgt ggagcatcca tcatatgggc ggcctcccgt caccgcctat cctacgacca
     1621 ttcggacggt ttggtaatac caaaatccgc acgatcgcgc cgatttcgcc gcccaggctt
     1681 gatttacaat ccgggtttac tttcacggga gagcgcagcg tggaactggg attgaaaggc
     1741 aaggtcgccg taatcaccgg cgcaacggaa ggcatcgggc gcgccacggc gctgaagctc
     1801 gcgcgggaag gcgcccgggt ggcaatctgc gcgcggcggc aggagccgct cgacacgacg
     1861 gcagccgaaa tcgggaagac cggggcggac gtgctcgccg tttccgccga catgagcaaa
     1921 gccgccgaca tcgagcgctt catgaaagcg gtgatcgacc gcttcggccg catcgacatc
     1981 ctcgtcaaca acgccggcac ctcggcccgc ggcaagttcc tcgagatcga ggacaagacc
     2041 tggagctctg acatcgagct gaaggtgttc ggcgcgatac gatgcacgcg gctcgccgtc
     2101 ccgcacatga agaagaacgg cggtgggcgc atcgtcaaca tcacgatctc gagcgccaag
     2161 cagccgggcg cggaatccta cccgacctcc gtttcgcgcg ccgcgggcct ggccctgacc
     2221 aaggcgctgt cgaaggaata cgcgccggac aacattcgcg tgaatacggt ctgcatcggc
     2281 aagatcaagt cggggcagca cgagcggcgc tacacccggg agcgcaagag cgccgacgcc
     2341 tactacaagg aagtgtcccg ggacatcccg ctcgggcggg tcggcgaggc cgagtaagtg
     2401 gcgaacgtga tcacgttcct ggtttcggat gcttcgagct acgtgaccgg caccagcgtc
     2461 aatctcgacg gg
//
LOCUS       DBNGSG010000499         7526 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_2032262, whole genome shotgun sequence.
ACCESSION   DBNGSG010000499 DBNGSG010000000
VERSION     DBNGSG010000499.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7526)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7526)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7526
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_2032262"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..84)
                     /locus_tag="AC158Q_13210"
     CDS             complement(<1..84)
                     /locus_tag="AC158Q_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HIR3340773.1"
                     /translation="MPGWVDAGFVEYAGRMPRETRIELVALK"
     gene            complement(133..489)
                     /gene="rsfS"
                     /locus_tag="AC158Q_13215"
     CDS             complement(133..489)
                     /gene="rsfS"
                     /locus_tag="AC158Q_13215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HIR3340774.1"
                     /translation="MRLDKLTKAAVAALDDIKGRDIVVLDVRKMTSLFDKIIVASADS
                     ARQGKALSKHLQDKLKALGARVHSVEGERTGEWILVDLGFLVVHIMQPAIRRHYNLEE
                     LWAPPPRARRRRAAAR"
     gene            complement(496..1152)
                     /gene="nadD"
                     /locus_tag="AC158Q_13220"
     CDS             complement(496..1152)
                     /gene="nadD"
                     /locus_tag="AC158Q_13220"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HIR3340775.1"
                     /translation="MSIAPIGVIGGTFDPVHYGHLRLAEELGETLKLGEVRFIPSGTP
                     PHRSAPQVAADHRLAMVRLAAEGNPRFAVDEREVRRAGPGYTFDTLAELRAETGASRP
                     LVLLLGADAFLEFATWHRWHEIFGLAHIAVAHRPGYPVERWSERMPQPLAREYSARLM
                     QQPLATHLQPAGGILVVPFTALDISATAIRDLLRAGASPRYLLPASVLDYIRQHQLYS
                     "
     gene            complement(1256..2629)
                     /gene="aceF"
                     /locus_tag="AC158Q_13225"
                     /pseudo
     CDS             complement(1256..2629)
                     /gene="aceF"
                     /locus_tag="AC158Q_13225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01348.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
     gene            complement(2696..5383)
                     /gene="aceE"
                     /locus_tag="AC158Q_13230"
     CDS             complement(2696..5383)
                     /gene="aceE"
                     /locus_tag="AC158Q_13230"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HIR3340776.1"
                     /translation="MDLKDILPDVDPQETKEWLEALASVLEREGPARANFLLDRLVEK
                     ARSHGAYIPYNANTAYMNTIPMGQEERSPGDAELEHKIRSLVRWNAMAMVLRANKESS
                     ELGGHIASFASAATLYDVGFNHFFRAWNDSFGGDLVYFQGHSAPGIYARAYLEGRITE
                     EQMNHFRQEVGGKGLSSYPHPWLMPDFWQFPTVSMGLGPLMAIYQARFMRYLQGRSLV
                     DTEGRKVWCYCGDGEMDEPESLGAISLAGRERLDNLIFVINCNLQRLDGPVRGNGKII
                     QELESDFRGAGWNVIKVIWGSYWDPLIMRDKNGLLLKRMEECVDGEYQAFKSHDGAFV
                     RKHFFGKYPELLEMVSHMSDDEIWRLNRGGHDPHKMYAAYAAAVKHKGQPTVILAKTV
                     KGYGMGESGEGQNITHQQKKMGTASIKAFRDRFNVPIPDDRLEEMPFYRPAEDTPEMK
                     YLRERIQAMGGSVPARRRKSESLEVPPLSAFETLLKGTEGREISTTMAFVRILNTLVR
                     DKKIGKRIVPIVPDESRTFGMEGMFRQLGIYSSVGQLYTPQDAEQLMYYKEDKSGQIL
                     QEGINEAGGMCSWIAAATSYSTNNQPMIPFYIFYSMFGFQRVGDLTWAAGDMRARGFL
                     LGGTSGRTTLNGEGLQHEDGHSHVLASTVPNCVSYDPTFAYELAVIIQDGMRRMVQDQ
                     EDVYYYITVMNENYAHPPMPEGAEAGILRGMHLISRPENAGKAAKGKLNVQLMGCGAI
                     LREVMAAAEMLEKDFGVSADIWSVTSFNELRRDGIETARWNLLHPEAEPRRSYVEQCL
                     RDHTGPVIAATDYMKVFADQIRAYLPQPAHYSVLGTDGFGRSDTRRALRGHFEVDRRY
                     VAVAALKALADQEALPQKKVVEAIRKYGVDPEKPNPAKA"
     gene            complement(5563..6348)
                     /locus_tag="AC158Q_13235"
     CDS             complement(5563..6348)
                     /locus_tag="AC158Q_13235"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR3340777.1"
                     /translation="MEFKNILLEQPEAGIYLLTVNRPKALNALNAATLDEIASAVKKV
                     AADDRARALLVTGAGDKAFVAGADIAEMKDATIEQARAFSEKGMHVMHALEAAPVPVI
                     ALVNGYCLGGGCEIALACDWILASENAVFGQPEVNLGIPPGFGGTQRLPRRIGPARAL
                     ELLTTARQVKAEEAAAIGLANHVYPVSELRAKGIEMARAIAAKGPAAVRLVKQAVRRG
                     ASLDLYTACALETDLFAQAFGTQDRREGMNAFLEKRPAKFRNS"
     gene            complement(6498..7037)
                     /locus_tag="AC158Q_13240"
     CDS             complement(6498..7037)
                     /locus_tag="AC158Q_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340778.1"
                     /translation="MGLSDFLKKLFPPGESPLLSMDPQCVRYQWLTLRLPAGWRFTQA
                     DGRSFSASGPGSCSVAFFFANVVKGAHKSGQRLRAQEFEDERKNLVQLMGKYFLEGKS
                     GGETILPTGIVWMEATDTQGQGKRLRIALLNPRPRNQEWIPPMLQVTCTTPGGSSRES
                     LGADQFEALRAALRGIEWN"
     gene            complement(7343..>7526)
                     /locus_tag="AC158Q_13245"
     CDS             complement(7343..>7526)
                     /locus_tag="AC158Q_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006483642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HIR3340779.1"
                     /translation="TPMVAGFAPELQQALAAQVPFPPRLGRPDEFAALVEHIITNVML
                     NGEVIRLDGAVRMSAK"
BASE COUNT         1202 a         2466 c         2363 g         1495 t
ORIGIN      
        1 cttgagcgcg acgagttcga tgcgcgtttc gcgcggcatc cggccggcgt attccacgaa
       61 gccggcgtcc acccagcccg gcatgcggtg accgaccgcg accacgatga atttcacggc
      121 aatgatccgc cgtcagcggg cggcggcgcg gcggcgccgg gcacgcggcg gcggcgccca
      181 caattcttcg aggttgtagt gcctgcggat ggccggctgc atgatgtgaa cgaccaggaa
      241 ccccaggtcc accaggatcc attctcccgt gcgctctcct tccacgctgt gcacgcgcgc
      301 gccgagcgcc ttcaatttgt cctggagatg cttggatagc gccttgccct ggcgggcgga
      361 gtcggcgctc gcgacgatga tcttgtcgaa cagcgaggtc atcttcctca cgtcgaggac
      421 cacgatgtcg cgccccttga tatcgtccag cgcggcgacc gcggccttgg tgagcttgtc
      481 gagtctcatc aacccctatg aatacagttg gtgttgccga atataatcga gaaccgaagc
      541 gggcagcaaa tatcgcgggc tcgccccggc gcgcagcagg tcgcggatcg cagtcgctga
      601 aatatcgagc gccgtgaacg ggacgaccag gatgccgccc gcgggctgca ggtgagtcgc
      661 cagcggctgc tgcatgagtc gcgccgagta ttcgcgcgca agcggctgcg gcatgcgctc
      721 gctccagcgc tcgaccgggt agccgggacg gtgcgcgacg gcaatgtgcg ccagcccgaa
      781 aatctcgtgc cagcggtgcc aggtcgcgaa ttcgagaaac gcgtccgcgc cgagcagcag
      841 cacaagcggg cgtgaagcgc cggtttccgc gcgcagttcg gcgagcgtat caaaggtgta
      901 gccggggccc gcgcggcgca cctcgcgctc gtccacggcg aagcgcgggt tgccctccgc
      961 cgccagacgc accatcgcca ggcgatggtc ggccgcaacc tgcggcgcac tgcggtgcgg
     1021 cggcgtgccg ctgggaatga atcgcacctc gccgagcttc agcgtctcgc ccagttcctc
     1081 ggccagccgc aggtgcccgt agtgaaccgg gtcgaaggtg ccgccgatga cgccgatggg
     1141 ggcgattgac atgcgtcaat cgccccagtg atgagtgatg agtgatgagt gatgagtggc
     1201 ccgcaccgcg gggacccggc tcgattcacg attcacgatt cacgactcac ggtgttcaaa
     1261 gcaccaggcg gcggatgtcg gtcaggaccg agctcaggaa ggaggtgaag cgcgcgccct
     1321 gcgcgccgtc gatcacgcgg tggtcgtagg aaaccgccag cggcagcatc agccgcggct
     1381 cgaaggcctt cccgttccag atcggctgca tgaccgattt tccgacgccg aggatcgcca
     1441 cctctggcgc gttgatgatg ggggtgaaat gggcgccgcc gatgccgccc aggctcgaga
     1501 tcgaaaaaca ccccccctgc aggtcgttgg gcgagatctt gcccgcgcgc atgcgcgcgc
     1561 tcgcttcgcc cagttccttc gccaggtcca tcagcccctt cttgtccacg tcgcgtatga
     1621 ccggcaccac caggccgttc ggggtgtcca ccgccacgcc gatgtggaag tactgcttga
     1681 ggacgaggtt ctccccgtcc acggacagcg aggcgttgaa ctcgggatgc tgcttcagcg
     1741 ccaccaccac cgctttcagc aggaagcaca ggatcgtgaa gcggatgccc tgcttcttcg
     1801 cctcctccgt ctgtatcttg cggaattcct cgagctcggt gatgtcggcc aggtcgttct
     1861 gcgtgacgtg cgggatcgtc acccagttgc ggtgcaggtt cgcgcccgcg agcttcttga
     1921 tgcgcgagag cggctgcgtg gtaacagcgc cgaacttcgc gaaatccacc tgcggcagcg
     1981 acagcaggtt gagccccgtt ccgccttccg tcccgcgcgg gcgcgagagc tcgcccttca
     2041 cgtaattctg gatgtcctcc ttgagcacgc gctccttcgg ccccgtgccc ttcacgcgcg
     2101 cgaggttcac gcccagctcg cgcgcgaaac gccgcgccga gggactcgcg tgcgcctggg
     2161 caaaaccggc ttcgtccacc cctcctccca ttcccccctt ggcaaggggg ggagacgggg
     2221 gggttgcggg tggcgccgat tgcggtggga gcgctggcga cggcgccgcg cggggcgcgg
     2281 gttgcggcgc cacccgctct tccttccccc ccttggcacg ggggggagtc gcgggcgggg
     2341 tggccgcgcc gtcgccgtcg gattccagca tgagcacgag cgtgccctcg gaaaccttgt
     2401 cgccgacctt gagcttgatc tccttcacca cgccggctgc gggcgcgggc acttccatcg
     2461 tcgccttgtc cgactcgagc gtgacgaggg gcgcctcctt gctgacgctg tcgcccggct
     2521 tgaccagtac ctcgatcacc gggctgttct tgaagtcccc gatgtcggga accttcactt
     2581 cgattacttt gctcatgcga gaatgggtgg ctcgttgctc gtttttcgtt gctcgtttct
     2641 caagcgtcgc cgttggttgt cacgagtaac gaggaacgag aaacgaatca cttctctagg
     2701 ccttggcggg attcggtttt tccggatcca cgccatactt cctgatcgcc tcgacgacct
     2761 tcttctgcgg cagcgcttcc tggtcggcaa gcgccttcag cgccgccacg gcgacgtagc
     2821 gccggtccac ctcgaagtgg ccgcgcagcg cccgccgcgt gtcggagcgc ccgaagccgt
     2881 cggtgccgag cacgctgtag tgcgccggct gcggcaggta cgcgcggatc tggtcggcga
     2941 acactttcat gtagtcggtt gccgcgatca ccgggccggt atggtccctc aggcattgct
     3001 cgacatagga gcgccgcggc tcggcctcgg gatgcagcag gttccagcgc gcggtctcga
     3061 tgccgtcgcg ccgcagctcg ttgaagctgg tgacgctcca gatgtcggcg gagacgccga
     3121 aatccttctc gagcatctcg gccgccgcca tcacttcgcg caggatggcg ccgcagccca
     3181 tgagctgcac gttcagcttg cccttggcgg ccttgcccgc attttccggg cgggagatca
     3241 aatgcatgcc gcggaggatg cccgcctcgg cgccttccgg catcggggga tgagcgtagt
     3301 tctcgttcat caccgtaatg tagtagtaga cgtcttcctg atcctggacc atgcggcgca
     3361 tcccgtcctg gatgatcact gccagctcgt aggcgaaagt cgggtcgtac gacacgcagt
     3421 tgggaacggt ggacgcgagc acgtggctgt gcccgtcctc gtgctgcagg ccctcgccgt
     3481 tcagcgtggt gcggccggac gtgccgccca gcaggaagcc gcgcgcgcgc atgtcgccgg
     3541 cggcccaggt gaggtcgccg accctctgga acccgaacat cgaatagaag atgtagaacg
     3601 ggatcatcgg ctggttgttg gtgctgtaag aggtcgccgc cgcgatccag gagcacatgc
     3661 cgcccgcctc gttgatgccc tcctgcagga tctggccgct cttgtcctcc ttgtagtaca
     3721 tcagctgttc cgcgtcctgc ggcgtgtaga gctggccaac cgatgagtag atgccgagct
     3781 ggcggaacat gccctccatg ccgaaggtgc gcgattcgtc cggcacgatg ggcacgatcc
     3841 gcttgccgat tttcttgtcg cgaacgaggg tgttgaggat gcgcacgaac gccatggtgg
     3901 tggaaatctc gcggccttcc gtgcccttca gcagggtctc gaacgccgag agcggcggca
     3961 cttcgagcga ctcgctcttc ctccgccgcg cgggcacgct gccgcccatc gcctggatcc
     4021 gctccctcag gtacttcatc tcgggggtat cctcggccgg ccggtagaac ggcatttcct
     4081 cgagcctgtc gtcgggaatc ggcacgttga agcggtcgcg gaacgccttg atcgaggccg
     4141 tgcccatctt cttctgctgg tgcgtgatgt tctgcccctc gcccgactcg cccatgccgt
     4201 agcctttcac ggttttggcg aggatcaccg tgggctggcc tttatgcttc accgccgcgg
     4261 cgtacgcggc gtacatcttg tgaggatcgt gcccgccgcg gttcagccgc cagatctcgt
     4321 catccgacat gtgcgagacc atctccagca attcgggata cttgccgaag aaatgcttgc
     4381 gcacgaaggc gccgtcgtgc gacttgaagg cctggtactc gccgtccacg cactcctcca
     4441 tgcgcttcag caggagcccg ttcttgtcgc gcatgatgag cggatcccag tacgagcccc
     4501 agatcacctt gatcacgttc cagccggcgc cgcggaaatc ggattccagc tcctggatga
     4561 tcttgccgtt gccgcgcacc ggcccgtcga gccgctgcag gttgcagttg atgacgaaga
     4621 tcaggttgtc gagccgctcg cggcccgcga gggaaatcgc gcccagcgac tccggctcgt
     4681 ccatctcgcc gtcgccgcag tagcaccaca ccttgcggcc ctcggtgtcg accaggctgc
     4741 ggccttgcag gtagcgcatg aagcgcgcct ggtagatcgc catgagcggc cccaggccca
     4801 tggacactgt cgggaactgc cagaagtccg gcatcagcca cgggtgcgga taggaagaca
     4861 agcccttgcc gccgacttcc tggcggaaat gattcatctg ttcctcggtg atgcggccct
     4921 ccagataggc gcgggcgtag atgcccggcg cggagtgccc ctggaaatag acgaggtcgc
     4981 cgccgaagct gtcgttccag gcacggaaga aatggttgaa gccgacgtcg tagagcgtgg
     5041 cggccgaggc aaagcttgcg atgtgcccgc ccagttcgga ggattccttg ttggcccgca
     5101 acaccatggc catcgcgttc cagcgcacca gggagcgtat cttgtgctca agttcggcgt
     5161 cccccggcga gcgctcttcc tggcccatcg ggatcgtgtt catgtacgcg gtgttggcgt
     5221 tgtacggaat gtaggcgccg tggctgcgcg ccttctcgac cagccggtcg agcaggaaat
     5281 tggcgcgcgc cgggccctcg cgctcgagca ccgaggcgag cgcctcgagc cattccttcg
     5341 tctcctgcgg atcgacgtcg ggcaggatgt ctttcaggtc catgacggcc accgtttcaa
     5401 ttgcaggagc ttctcttgtg agggggctgc cggcgctcgt cttgccgttc gccgggggaa
     5461 ggcattatag catcgggatt ttccccggcc ggacgcgata tccctttgtt cggtatggtg
     5521 aaagcggttt gcgcgatgcg gcgcgcccgc cgccgctacg ggtcacgaat tccggaattt
     5581 cgcggggcgc ttctccagga aggcgttcat gccttccctg cggtcctgcg taccaaaggc
     5641 ctgcgcgaac aggtcggtct ccagcgcgca ggcggtgtag agatcgaggc tcgccccgcg
     5701 ccgcaccgcc tgcttgacga gccgcaccgc ggccgggccc ttggccgcga tcgcgcgcgc
     5761 catttcaatt cctttcgcgc gcaattcgga tacgggataa acgtggttcg caagcccgat
     5821 ggcggccgct tcttccgctt tcacctgccg cgcggtcgtg agcaactcca gcgcgcgcgc
     5881 cggcccgatt ctccgcggca gccgctgcgt gccgccgaag ccgggcggta tgcccaggtt
     5941 cacttccggc tggccgaaca cggcgttctc cgaggcgagg atccagtcgc aggcgagcgc
     6001 gatctcgcag ccgccgccca ggcaatagcc attcaccagc gcaatcaccg gcaccggggc
     6061 cgcttccagc gcatgcatga cgtgcatgcc cttttcggaa aacgcccgcg cctgctcgat
     6121 cgtggcgtcc ttcatctcgg cgatgtccgc gcccgcgacg aacgctttgt ccccggcgcc
     6181 ggtgacgagc aacgcgcgtg ccctgtcatc cgccgcgact ttttttaccg cggacgcgat
     6241 ctcgtccagc gtcgcggcgt tcaacgcgtt cagcgccttc ggtcgattca ccgtcagcag
     6301 gtatatcccc gcctccggtt gctcgagcag aatgttttta aattccataa aacgctaaat
     6361 tagccgccaa gacgccaaga ccgccaggaa aatctattca acaagaatta acgtccacgg
     6421 tgacccgcgc gcggggtttg cgcgtcggtg tcgaccgtgt tgttcgatgt ctcgatgtcc
     6481 gcttggtcca ccgcagctta gttccactca ataccgcgta atgccgcgcg taacgcctca
     6541 aactggtcag caccgagact ctctctagac gatcctcccg gtgtcgtgca tgtcacctga
     6601 agcatcgggg ggatccattc ctggttccgc ggccgaggat taagaagcgc aatccgcagg
     6661 cgtttgccct ggccctgggt atcggttgcc tccatccaca caatgcccgt tggcagaatc
     6721 gtttcacctc ctgacttgcc ttccagaaag tatttgccca tgagctggac caggttctta
     6781 cgctcgtcct caaactcctg cgccctcaag cgttggcctg atttgtgggc tcccttgact
     6841 acgttcgcga agaagaacgc caccgagcag cttccggggc cggacgcgct gaagcttcga
     6901 ccatctgctt gtgtaaaccg ccaccccgcg ggcaggcgca gcgtgagcca ttggtatcga
     6961 acgcactgcg ggtccatgga cagcagcgga ctctcaccgg gaggaaacag tttctttagg
     7021 aaatcagaga ggcccatgcg tcctccgaaa gtatcgaact tgtattaggc gacatttacc
     7081 cagtgaagaa aagtatatac cggtagtgat atttcacgat ttgtagtgat gaatccccac
     7141 taatactgag tcgtaatcag gaaggatttt cttcctgtat ttcctggcgt gcttggcggc
     7201 cttggcggtt caggtcttcg attttcgatg cctggcgtct tggtggttcg ctgttgttat
     7261 ttttgtattc cttcgtgtcc tttgtgtcct tcgtgtcttc gcttttcctc tgtgtcctct
     7321 gtgtcctctg tgtctcagct ttttacttcg ccgacatcct taccgcgcca tcgagccgga
     7381 ttacctcgcc gttcagcatc acgttggtga tgatgtgctc caccagcgcc gcgaattcgt
     7441 ccgggcggcc cagccgcggc ggaaacggca cctgcgcggc gagcgcctgc tgcaattcgg
     7501 gcgcgaatcc cgctaccatc ggcgtc
//
LOCUS       DBNGSG010000500        10271 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1861815, whole genome shotgun sequence.
ACCESSION   DBNGSG010000500 DBNGSG010000000
VERSION     DBNGSG010000500.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 10271)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10271)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10271
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1861815"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="AC158Q_13250"
     CDS             <1..552
                     /locus_tag="AC158Q_13250"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:NF014418.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HIR3340780.1"
                     /translation="RRPELWGAHGWERLSPDGRGARIVPDESARRQLREGERRARALA
                     REGARVETKAASVALHWRGAPALAAAKMRDSLPHAWGPLTAEGDVELLPFDGGVELRA
                     RGRSKKHAVETMLAETADNGAVAYLGDDLTDEDAFRAVKPRGLAVLVRAELRETAADM
                     WIRPPRELIAFLRRWRDACGSRQ"
     gene            549..1616
                     /locus_tag="AC158Q_13255"
     CDS             549..1616
                     /locus_tag="AC158Q_13255"
                     /inference="COORDINATES: protein motif:HMM:NF044972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR3340781.1"
                     /translation="MKGWGEFLQAHGATDFAIAIAVALGVWVAFGVAKHFGGRALAHF
                     ARRTAARWDDIIAEAVQATNLLLLLPVALYAGSLVLELPARLGRVVELAALMVLMLQA
                     GLWVNRFIGHWAEYKIESSRAHEPQGATALGLIAFAARVAAWSFLLLLDLDQLGFDIT
                     ALVAGLGIGGVAVALAMQNILGDLFASLSIILDKPFVVGDFIVVDDLRGTVERIGIKT
                     TRLRSLDGELLVFSNADLLKSRVRNFQRMMERRVLFTVGVTYQTPPEQLRRIPQWLRE
                     AVEAQPRTRFDRAHFKEYGDSALVFEIVYYILDRDYNLFMDVQQAVNLSIFERFAREG
                     VEFAYPTHMVHLKQEPAAQPA"
     gene            1619..3142
                     /locus_tag="AC158Q_13260"
     CDS             1619..3142
                     /locus_tag="AC158Q_13260"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase
                     (UDP-forming)"
                     /protein_id="HIR3340782.1"
                     /translation="MDKAVSAGMTRRRDRLVVVSNRLPFSFQRAANGRWKAESSGGGL
                     VTALLPVLRHRGGMWIGWTGAADDSPELSHEVAAVGENAGYTLKNVSLTAEEVSHFYE
                     GFSNEIIWPLFHDMQLLCNFDPAYWRTYCEVNRKYARAVHDASAPGDFVWVHDYHLMN
                     VAAELRRLEMHARTGFFLHIPFPSPDIYLKLPWRMTLIEALLQFDLIGFQTARDRRNF
                     LECVRLLVKGADVEGRGQVVQVATGGRKVRVGNFPISIDFNAFMRQAAAPEVAARARE
                     LHSLLPNRKLILGIDRLDYTKGIPLRLQAFQNVLTRYPALRERISLIQVVVPSREDIP
                     EYRDLKTEIEQRVGKINGSFVVPGGWVPVWYVYRSLSRLDLLAYYRAADIALITPLKD
                     GMNLVAKEYCACSIEEDCALILSEFAGAAAQLGRGALLVNPCDIEGVADAIHRAYHMD
                     TRERQARMRSMRRSIRENDVFWWVDSFLRAAITKDLSAFPQLESSGANEDMEYTLTF"
     gene            3169..4371
                     /locus_tag="AC158Q_13265"
     CDS             3169..4371
                     /locus_tag="AC158Q_13265"
                     /inference="COORDINATES: protein motif:HMM:NF013188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR3340783.1"
                     /translation="MTSFLPDYPFPASPVLLFGLLLLTGLIGGELVKRVLSLPRIIGY
                     VATGLLLGGSGVNLLDQGLVNESWIFVDIALGLVLFELGRRLHFAWLRNDRWLLATGL
                     LESALAFAFVYFALVYFDVQPIYAAVAAAIGVSTSPAVVLLVAQELKAEGQVTERALN
                     LVAMNSVIAFVLTTMLLTWIHHEYQAGWSTIALHPVYLLGGSLILGYAASMAVILLSR
                     WLGKHAERQLVMLLAVIVLTVGAARMLELSVLLSLLSFGVLSRNLDERHDLMAVDLSS
                     VGQMFYVVLFVVSGAKLQLVELMTGGAIAAVYVVARFVGKSIGVMSLTYLSGARAGAA
                     GLLCIALTPMSGIALAMVHETANLYPRFGAQLASIVLSAALILELVGPVAVQFALKRA
                     GEAKDAAA"
     gene            4368..5504
                     /locus_tag="AC158Q_13270"
     CDS             4368..5504
                     /locus_tag="AC158Q_13270"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - modified amino acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HIR3340784.1"
                     /translation="MSGPEMNFTPSKNLSIGVELELQILNSRDFDLARDAADLIGLLE
                     KSALPGAVKPEITESMVELNSSIHQGYDTLVAELRTIRDAVAQAARRLNVRIAGGGSH
                     SFHSWADRRIYPTERFNHLLAVYGYLAKQFTIFGQHVHIGCPDGDAAIYLTHILSRYV
                     PHFIALSASSPFQQGEDTAYQSSRLNTVSAFPLSGQIPFVHSWNEFLEYYDRMRGYGI
                     VESMKDFYWDIRPKPEYGTVEIRVFDTPLTVERAALMAAYAQSLARHILADRPLAPSR
                     DVYLLYNYNRFQACRYGMESRIVDAYTQRQTGMKEDILGTLSVIAPHAAELGNAQALA
                     RLRAEIEGGRTDASWLRGIYKERGSLNDVARMQSDLWMGLDKRA"
     gene            5524..6351
                     /locus_tag="AC158Q_13275"
     CDS             5524..6351
                     /locus_tag="AC158Q_13275"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019342.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HIR3340785.1"
                     /translation="MTRKPLRIGLSPRFLHTAPRELGFRGKTLQYLEQTVAHWLMTAG
                     VLVFMLPSIAGGGLLRRGNLRIADYVAELDGLVLQGGADVSPLTYGETPLRSKWEGDR
                     VRDVYELDLLQAFVARGKPVLGICRGLQLINVAYGGTLYQDLALHHPRAGDHHDPETY
                     DQHFHDIALVAGSGLARIYSGRGHARVNSIHHQGIRILGKDLFSEAVSMPDQVIEAIR
                     WNGPGYVVGVQWHPEFHDPANADLLDGGPLLQEFLECAEAGRERVARKPAAEPAGPA"
     gene            6628..7782
                     /locus_tag="AC158Q_13280"
     CDS             6628..7782
                     /locus_tag="AC158Q_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR3340786.1"
                     /translation="MLKKFLKKPAALPEKESASRRKFLTGAAAATTGAAITGFPMISV
                     AQTPVVIKMQGAWGAKDIFNEMAQEYVTRVNEMAGGRLRIDYLVAGAVVKPFEVMDAT
                     SKGVLDANHSVPVYWYGKSKAASLFGSGPINGMDAHQTMAWIYRGGGLELYNELLAKL
                     NLDVVGFFALPMPTQPLGWFRKPIKDAAGLKGLKYRTVGLAADLFQEMGAKVTQLPGG
                     EIMPALERGVIDAFEFNNPTSDMRFGAQDAIKNYMMGSFHQAMEFFEIVFNKKKWNSL
                     PKDLQAILRYGAEAANSANYWTGIDNYSRDLQILKDKHKVNVIRTPRSIFQEQLKAWD
                     VITPKLSKEDPFFAKVVASQKAFAKRAAYYVWLNEADYKLGYEHVFKTKI"
     gene            7886..8488
                     /locus_tag="AC158Q_13285"
     CDS             7886..8488
                     /locus_tag="AC158Q_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HIR3340787.1"
                     /translation="MINRFVYAVDMLSRTVGHAFAWCLIILTLGTCYEVFVRYVLNAP
                     TSWAFDFSYLMYGAMFYMAGAYTLSRGGHVRADMFYRLWRPRTQASVELVLYVIFFFP
                     GVLALVISGWHYGYDSMRILESSVNSPAGVPIWPLKMLIPFGAGLLALQGFADVLRCI
                     VCIRNNEWTARLHDVEELEKQILEEYARKAERDAAQGATR"
     gene            8488..9834
                     /locus_tag="AC158Q_13290"
     CDS             8488..9834
                     /locus_tag="AC158Q_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR3340788.1"
                     /translation="MDPAVVALVMLGILVITIMIGFPVAFTLMALGVMFGYWAYFQPD
                     QAFFDNRVFYLLTQNTFTTMNNDVLVAVPLFLLMGYMVERSNLLDSLFHSVQLALKRL
                     PGSLAVATLITCALFATATGIVGAVVTLMGLLAFPQMLKAGYDVRIASGVTCAGGCLG
                     IMIPPSILLIVYAAMAGMSVVQLYAAALIPGFLLAGMYVVYVIVLATLKPHLMPPLPK
                     EQGNVPVREVLLLLLKSFVPLALLILSVLGTIMFGLATPTEAAAVGAFGSMLLAAAYR
                     SFTWGRLKESVYLTARTSAMVCFLFVGSATFAGVFGYLGGQAIIEKFVLGLDMNPITF
                     LLMAQFIIFLLGWPLEWTEIIIIFVPIFLPMLKHFEIDPLIFGILVALNLQTAFLSPP
                     MAMAAYYLKGIAPPHVLLTQIFRGCMPFLYIVLVSMLFVYVIPQTVTWLPRQIYGN"
     gene            9838..>10271
                     /locus_tag="AC158Q_13295"
     CDS             9838..>10271
                     /locus_tag="AC158Q_13295"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HIR3340789.1"
                     /translation="MDASQLNWLSATDAARAVRDGAVSAEQLVAACLARIRESEPQVQ
                     AWQHLDPEHALAQARALDERHRQGESAGPLHGVPVGVKDIIDTADMPTEDGTVLHAGR
                     TPERDAAVVAMLRAAGAVIMGKTVTTECATYTAGKTRNPHNP"
BASE COUNT         1695 a         3246 c         3431 g         1899 t
ORIGIN      
        1 cgccgcccgg agctgtgggg ggcgcacggc tgggagcggt tgtcgcccga cggccgcggc
       61 gcgcgaatcg ttccggacga gagcgcgcga cggcagctgc gcgagggcga gcgccgggcg
      121 cgcgcgctgg cgcgcgaggg cgcccgagtc gagaccaagg ccgcaagcgt cgcgctgcac
      181 tggcgtgggg cgcccgctct cgcggcggcg aagatgcggg attcgttgcc gcatgcctgg
      241 gggccgctca cagccgaggg cgacgtcgaa ttgctgccat tcgatggcgg cgtggaactg
      301 cgggcgcgcg gccgaagcaa aaagcacgca gtggagacga tgctcgcgga gacggccgac
      361 aacggcgcag tcgcatacct gggcgacgat ctgaccgacg aagacgcgtt tcgcgcggta
      421 aaaccgcgcg gcctggcggt tctcgtgcgc gccgagctgc gcgaaacggc ggcggacatg
      481 tggattcgcc cgccgcgcga gctgatcgca ttccttcggc gctggcgcga tgcctgcgga
      541 agccggcaat gaagggttgg ggcgagtttc tgcaggcgca cggagcgacg gatttcgcga
      601 tcgcgatcgc ggtcgcgctc ggtgtctggg ttgccttcgg ggttgcgaag cacttcgggg
      661 gacgtgcact ggcgcatttt gcgcgtcgca cggcggcccg ctgggacgac ataatcgcgg
      721 aggcggtgca ggcgacgaac ctgctgctgc tgttgcccgt cgccctgtac gccggcagcc
      781 ttgtgctcga gttgccggcc cgcctgggcc gcgtggtcga gctggccgcg ctgatggtgc
      841 tgatgctgca ggccggattg tgggtgaacc ggttcatcgg gcactgggcg gagtacaaga
      901 tcgaatccag ccgcgcgcac gagccgcaag gcgccaccgc gctcggcctc atcgcatttg
      961 cggcacgggt cgcggcgtgg tcgtttttgc tgctgctcga cctcgatcaa ctgggcttcg
     1021 acatcaccgc gctggttgcg gggctcggca ttggcggcgt ggcggtggcg cttgccatgc
     1081 agaacatcct tggtgacctg ttcgcttcgc tttcgatcat tctggacaag ccgttcgtcg
     1141 tcggcgattt catcgtggtg gatgacctgc gcggcacggt ggaacggatc ggcatcaaga
     1201 ccacccgctt gcgcagcctc gacggtgagc tgctcgtttt ttccaatgcc gacctgttga
     1261 agagccgcgt tcgcaacttc cagcgtatga tggagcgtcg ggtcctgttc acggtgggcg
     1321 tgacctacca gacgccgccg gagcaactga ggcggattcc gcagtggctg cgcgaggcgg
     1381 tcgaggcgca gccgcgcacg cgcttcgacc gggcgcactt caaggaatac ggggactccg
     1441 cgctggtgtt cgagatcgtc tattacatcc tcgatcgcga ctacaacctg ttcatggatg
     1501 ttcagcaggc cgtcaatctg tcgatcttcg agcggttcgc gcgcgaaggc gtcgagttcg
     1561 cctatccgac gcacatggtg catctgaaac aggagccggc ggcgcagccg gcctgagcat
     1621 ggacaaagcg gtgagcgcag ggatgacccg tcggcgcgat cgcctggtcg tggtgtcgaa
     1681 ccggctgccg ttttcgttcc agcgggcggc gaacggccgc tggaaagcgg agtcgagtgg
     1741 cggcgggctg gtgacggcgt tgttgccggt gctgcggcat cgcggcggca tgtggatcgg
     1801 ctggacgggg gccgcggacg actcgccgga actgagccac gaagtcgcag cggtcggcga
     1861 gaacgccggt tacacgctga agaacgtgtc gctgaccgcc gaggaggtga gtcatttcta
     1921 cgagggcttc tcgaacgaga tcatctggcc cctgtttcac gacatgcagt tgctgtgcaa
     1981 tttcgacccc gcctactggc gcacctactg cgaggtgaac cgcaagtacg cccgggcggt
     2041 gcacgacgcg agcgcgccgg gggatttcgt atgggtgcac gattatcacc tgatgaacgt
     2101 ggcggccgag ctgcgccgtc tcgaaatgca tgcccgcacc gggttcttcc tgcatatccc
     2161 gtttccgtcg cccgacatct atctcaagct gccgtggcgc atgacgctca tcgaggcgct
     2221 gctgcagttc gacctgatcg gctttcagac cgcgcgcgac cgccgcaact tcctggagtg
     2281 tgttcgcctg ctggtcaagg gcgccgatgt cgagggccgc gggcaggtgg tccaggtcgc
     2341 aaccggaggc cgcaaggtgc gcgtcggaaa tttccccatc agcattgatt tcaacgcctt
     2401 catgcggcag gccgcggcgc ccgaggtggc ggctcgcgcc agggagctgc actcgctgct
     2461 gcccaatcgc aagctgatac tcggcatcga ccgcctcgat tacaccaagg gcattccact
     2521 caggctgcag gcattccaga acgtactgac gcgctatccg gcgctgcgcg agcgcatttc
     2581 cctcatccag gtggtggtgc cgagccggga agacataccg gagtaccggg acctcaagac
     2641 ggagatcgag cagcgcgtcg gaaagatcaa cggcagtttc gtggttcccg ggggctgggt
     2701 gccggtgtgg tacgtctatc gaagcctgtc gcggctcgac ttgctggcgt actaccgcgc
     2761 agccgacatc gcgctcatca ccccgttgaa ggacggcatg aacctggtgg cgaaggaata
     2821 ttgtgcgtgc agcatcgagg aagactgcgc gctgatcctg tcggagttcg ccggcgcggc
     2881 ggcgcagctc ggacggggcg cactgctggt gaatccctgt gacatcgagg gcgttgcgga
     2941 tgccattcac cgggcttacc acatggacac ccgcgaacgc caggcgcgca tgcgcagcat
     3001 gcgccgctcg atccgcgaaa acgatgtgtt ctggtgggtg gactcgttcc tgcgcgccgc
     3061 gatcaccaag gacctgagcg cgtttcctca gctggagagt tccggcgcga acgaggatat
     3121 ggaatatacg ctgacgttct gattgcgggg gggcataatc cacggtgcat gacgagcttt
     3181 cttcccgact atcccttccc cgccagcccg gtgctgctgt tcggcctcct gctgttgacg
     3241 ggcctcatcg gcggcgagct ggtgaagcgc gtgctgagcc tgccgcggat catcggctac
     3301 gtggcgacgg gcctgctgct cggcggcagc ggcgtgaacc tgctcgatca agggctggtc
     3361 aacgagtcgt ggatcttcgt cgacatcgcg cttggcctgg tgctgttcga gctggggcgg
     3421 cggctccact tcgcgtggct ccgtaacgac cgctggctgc tggccacggg cctgctcgag
     3481 agcgcgctgg ccttcgcgtt tgtctacttc gcgctggtct actttgacgt gcagccgatc
     3541 tacgccgcgg tcgccgcggc gatcggtgtc tcgacttcgc cggcagtggt cctgctggtg
     3601 gcgcaggagc tgaaagccga gggacaggtc accgaacggg cgctgaacct cgtggcgatg
     3661 aacagcgtga tcgcgttcgt gctgacgacg atgctgttga cctggataca ccacgagtac
     3721 caggcaggct ggtccaccat cgcgctgcat cccgtctatc tcctcggcgg ctcgctgata
     3781 ctgggctacg cggcgagcat ggcggttatc ctgctgtcgc gctggcttgg caagcatgcc
     3841 gagcgccagc tcgtgatgct gctggccgtg atcgtgctca ccgtcggggc cgcgcgaatg
     3901 ctggagctct cggtgctgct ttccctgctc tccttcggcg tgctctcccg caacctggac
     3961 gagcgccacg acctgatggc ggtggacctg tcgagcgtcg ggcagatgtt ctacgttgtg
     4021 ctgttcgtgg tgagcggcgc caagctgcag ttggtcgaat tgatgaccgg gggcgcgatc
     4081 gccgcggtgt acgtggtggc gcgtttcgtc ggaaagtcga tcggcgtcat gagcctgacc
     4141 tacttgagtg gcgcccgcgc gggcgcggcg ggcctgctgt gcattgcgct cacgccgatg
     4201 tcggggatcg cgcttgcgat ggtgcacgaa accgcgaatc tctatccccg gttcggcgcg
     4261 caactggcgt ccatcgtgct cagcgcggcg ctgatcctgg aactggtcgg gccagtcgcg
     4321 gtccagttcg cgctgaagcg cgctggagaa gcgaaggatg cggcggcatg agcggcccgg
     4381 aaatgaattt cacgccatcg aaaaacctgt cgatcggcgt cgagctcgag ctgcagatcc
     4441 tcaactcgcg cgatttcgac ctcgcgcgcg acgcggccga tctgatcggg ctgctcgaga
     4501 agagcgcgct tcccggcgca gtcaagcccg agatcaccga gagcatggtg gagctgaaca
     4561 gctcgataca ccagggatac gacaccctcg tcgcggagct gaggacgatc cgcgacgcgg
     4621 tggcccaggc ggcacggcgg ctgaacgtcc gcatcgcggg cggcggatcc cactccttcc
     4681 actcgtgggc ggatcggcgc atctacccga cggagcgctt caatcacctg ctggcggtct
     4741 atggttacct ggccaagcag ttcacgatat tcggccagca cgtgcacatc ggctgcccgg
     4801 acggggacgc tgcgatctac ctgacccaca tcctgtcgcg ctacgtgccg catttcatcg
     4861 cgctctccgc ctcctcgcca ttccagcagg gcgaagatac cgcctaccag tcatcccgtt
     4921 tgaacacggt gagcgcgttc ccgctatcgg ggcagatccc gttcgtgcat tcgtggaacg
     4981 agtttctcga gtattacgac cggatgcgcg gatacggcat cgtcgagagc atgaaggatt
     5041 tctactggga tatccggccc aagcccgagt acgggacggt cgagatccgc gtgttcgata
     5101 cgccgctcac ggtcgagcgc gccgcgctga tggccgccta cgcccaatcg ctggcccgcc
     5161 acatactggc cgaccggccg cttgcgccgt cgcgcgatgt ttacctgctc tacaactaca
     5221 accgcttcca ggcgtgccgt tacggcatgg agagccgcat cgtcgacgcc tacacgcagc
     5281 ggcagacggg catgaaggag gacattcttg gaacattgtc cgtgattgcg ccgcacgcgg
     5341 ccgagctggg caacgcgcag gcgctcgccc gtctccgggc ggaaatcgag ggcgggcgca
     5401 cggatgccag ctggctgcgc ggcatctaca aggagagggg ctcgctcaac gacgtggccc
     5461 gcatgcaaag cgacctgtgg atggggctcg acaagcgggc atgacgatgg cgagacgcgc
     5521 gccgtgacac gcaaaccgct caggatcggc ctgtcgcccc gcttcctgca caccgcgccg
     5581 cgggaactcg gcttccgcgg caagacgctg cagtacctgg agcagaccgt cgcgcactgg
     5641 ctgatgacgg ccggcgtgct ggtattcatg ctcccgtcca tcgcgggcgg tggcctgctg
     5701 cgtcgcggaa acctgcgcat tgccgattac gtggcggagc tggacggcct ggtcctgcag
     5761 ggcggcgccg acgtgagccc gcttacctac ggcgagaccc cgttgaggag caaatgggaa
     5821 ggcgaccgcg tgcgcgacgt ctacgagctc gatctgctcc aggcattcgt cgcgcgcggc
     5881 aagccggtgc tcggcatctg ccgcgggctg cagctcatca acgtcgccta cggcggcacg
     5941 ctctaccagg atctcgcgct gcatcatccc cgggccgggg accaccacga ccccgaaacc
     6001 tacgaccagc atttccacga tatcgcactg gtcgccggtt ctgggctggc gcggatctac
     6061 tcgggccggg gccacgcgcg ggtgaacagc atccaccacc agggcatccg catcctgggc
     6121 aaggatctct tcagcgaggc ggtgtccatg ccggaccagg tgatcgaggc gatccgctgg
     6181 aacggcccgg gctatgtcgt cggcgtgcaa tggcacccgg aatttcacga ccccgcgaat
     6241 gccgacctgc tcgatggcgg gccgctgctg caggaatttc tcgagtgcgc ggaagccggc
     6301 cgcgaacgcg tcgcgcgcaa gccggctgca gagccagccg ggccggcttg atggagccat
     6361 cctgcgccca attgcagtgc cagggcgcgc aaccggcggt gtcagattgc gtaagtatcc
     6421 ggtgctatcg ggcggggctc cggcggcttg catttgcgag tgacggagta taggattgcc
     6481 gcagcgtagt agagcttccc gcatactaaa atccggcatt ttcctgcagg gtccatgcag
     6541 atggttccac gcagaatcag ggtgctttgc tgccgctggc ccggtaacat gtttttcccc
     6601 gttacataac agaggatgga ggacgtaatg ctcaagaaat tcttgaagaa accggcagcc
     6661 ttgccggaaa aggagagcgc atcccgccgc aagttcctga ccggcgcagc ggcagcgacc
     6721 accggtgcgg cgatcaccgg cttcccgatg atttccgtgg cgcagacacc cgtggtgatc
     6781 aagatgcagg gcgcatgggg cgcgaaggac atcttcaacg agatggccca ggaatacgtg
     6841 acgcgcgtca acgagatggc gggcggcagg ctgcgcatcg attacctcgt ggcgggcgcg
     6901 gtggtgaagc cgttcgaggt gatggacgcg accagcaagg gcgtgctcga cgccaaccac
     6961 agcgtgccgg tgtactggta cggcaagtcg aaggccgcgt cgctgttcgg ctccggcccg
     7021 atcaacggca tggacgcgca ccagacgatg gcctggatct accgcggtgg cgggctggag
     7081 ctctataacg agctgctcgc caagctgaac ctcgacgtgg tcggattctt cgcgctgccg
     7141 atgccgaccc agccgctcgg ctggttccgc aagccgatca aggacgcggc cggactgaaa
     7201 ggcctcaagt accgcacggt gggcctcgct gcggacctgt tccaggagat gggagcgaag
     7261 gtgacccagc tgccgggcgg cgaaatcatg ccggcgctcg agcgcggcgt tatcgacgcc
     7321 ttcgagttca acaacccgac ctccgacatg cgcttcggcg cgcaggacgc gatcaagaac
     7381 tacatgatgg gcagctttca ccaggcgatg gagttcttcg aaatcgtctt caacaagaaa
     7441 aagtggaact cgctgccgaa ggacctgcag gcgatcctgc ggtatggggc cgaggcggcc
     7501 aattcggcga actactggac ggggatcgac aactactcgc gggacctgca gatcctgaag
     7561 gacaagcaca aggtcaacgt gatccgcacg ccgcggtcca ttttccagga gcagttgaag
     7621 gcgtgggacg tgatcacgcc caagctgtcg aaggaagatc cgttcttcgc caaggtcgtg
     7681 gcatcgcaga aggcattcgc caagcgcgcc gcctactacg tgtggctgaa cgaagcggac
     7741 tacaagctgg gctacgagca cgtgttcaag accaagatct gagtccggga cgcatcgcat
     7801 ttcggttcaa gccctaacgg aacaggggcg gtgcctcgcg gtaccgcccc gtttttctgg
     7861 tcatcggttt tgatcgtcga tcttcatgat taaccgcttc gtctacgccg tcgacatgct
     7921 gagcaggacc gtcgggcatg ccttcgcctg gtgcctgatc atcctcaccc tcggcacctg
     7981 ttatgaagtc ttcgtgcgct atgtcctgaa cgcgccgacg agctgggcat tcgacttcag
     8041 ctacctgatg tacggcgcca tgttctacat ggccggcgcc tacacgctgt cgcgcggcgg
     8101 ccacgtgcgc gccgacatgt tctaccgcct gtggcggccg cgcacccagg cgtcggtcga
     8161 gctcgtgctt tatgtcattt tctttttccc cggcgtgctg gcgctggtga tctcgggctg
     8221 gcattacggc tatgactcga tgaggatcct ggagtcgagc gtcaacagcc ccgcgggcgt
     8281 gccgatctgg ccgctcaaga tgctgatacc tttcggcgcc ggcctgcttg cgctccaggg
     8341 cttcgccgac gtgctgcgct gcatcgtctg catacggaac aacgagtgga cggcccgact
     8401 gcacgacgtc gaggagctcg agaagcagat cctcgaggag tacgcaagga aggcggagcg
     8461 ggatgccgcc cagggagcga cgcgataatg gatccggccg tcgtcgccct cgtaatgctg
     8521 gggatcctgg tcatcacgat catgatcggg ttcccggtcg ccttcaccct gatggcgctg
     8581 ggcgtgatgt tcggctactg ggcttacttc cagccggacc aggcgttttt cgacaaccgc
     8641 gtgttctacc tgctgacgca gaacaccttc accaccatga acaacgacgt gctggtggca
     8701 gtaccgctgt tcctgctgat gggctacatg gtggagcgct cgaacctcct cgacagcctg
     8761 ttccacagcg tgcagctcgc gttgaaacgc cttccgggtt cgctggccgt cgccacgctg
     8821 atcacctgcg cgctgttcgc caccgccacg ggaattgtcg gcgcggtggt gaccctgatg
     8881 gggctgctcg cttttcccca gatgctcaag gcgggctacg acgttcggat cgcctcgggc
     8941 gtgacctgcg ccggcggctg cctggggatc atgatcccgc cgtccatcct gctgatcgtc
     9001 tatgccgcga tggcgggcat gtcggtggtc cagctctacg ccgccgcgct gatccccggt
     9061 ttcctgctcg ccggaatgta cgtcgtctac gtaatcgtgc tcgcgacgct caagccgcac
     9121 ctcatgccgc ccctgcccaa ggagcagggc aacgtgccgg tgcgtgaggt gctgctgctg
     9181 ctgctcaagt ccttcgttcc gctggcactg ctgatactgt cggtgctggg cacgatcatg
     9241 ttcgggctgg cgacgccgac cgaggcggcg gccgtgggcg cgttcggctc gatgctgctg
     9301 gcggcggcct accgctcgtt cacctggggc cggctgaagg agtcggtgta cctcacggcg
     9361 cgcacgtcag cgatggtgtg cttccttttc gtcggctccg caaccttcgc cggggttttc
     9421 ggctacctcg gcggccaggc catcatcgag aagttcgtgc tcggcctcga catgaacccg
     9481 atcaccttcc tgctgatggc gcaattcatc atcttcctgc tgggctggcc gctggaatgg
     9541 accgagatca tcatcatctt cgtgccgatc ttcctgccga tgctgaagca cttcgagatc
     9601 gacccgctga ttttcggcat tctggtggcg ctgaacctgc agaccgcgtt cctgtcgccg
     9661 ccgatggcga tggccgccta ctacctcaag ggcatcgcgc cgccgcacgt gctgctgacg
     9721 cagattttcc gcggctgcat gccgtttctc tacatcgtgc tcgtgtccat gttgttcgtg
     9781 tatgtcatac cgcagaccgt gacctggctg ccaaggcaga tttacggcaa ttgagcgatg
     9841 gacgcctccc agcttaactg gctttccgca accgatgccg cgcgcgccgt gcgcgacggc
     9901 gccgtgagcg cggagcaact ggtcgcagcc tgtctcgcgc gcatccgcga gtccgagccg
     9961 caggtgcagg cctggcaaca cctcgatccg gagcacgcgc tggcacaggc gcgcgcgctc
    10021 gacgagcgcc atcgccaggg cgagtcggcc gggccgctgc acggcgtgcc ggtgggcgtc
    10081 aaggacatca tcgataccgc ggacatgccc accgaggacg gcaccgtcct gcacgctggc
    10141 cgcacgccgg agcgcgatgc cgccgtcgtc gcgatgctgc gcgccgcggg cgcggtgatc
    10201 atgggcaaaa ccgtcaccac cgagtgcgcc acctatacag cgggcaagac gcgcaatcct
    10261 cacaaccccg a
//
LOCUS       DBNGSG010000501         4071 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_674260, whole genome shotgun sequence.
ACCESSION   DBNGSG010000501 DBNGSG010000000
VERSION     DBNGSG010000501.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_674260"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="AC158Q_13300"
     CDS             <1..634
                     /locus_tag="AC158Q_13300"
                     /inference="COORDINATES: protein motif:HMM:NF016241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HIR3340790.1"
                     /translation="VTAASGNTDSVTYAVNGDAVRHTDKDKINGYLQSTYGRREVNGI
                     TERSSDLIRAGTKYDRNINDRTFGFGALDLERDQLINLDLRSVVAGGLGYHVIKREGL
                     TFDVSSGPAYNRERYSTDTREFMEWLFAEESIHALTPTISFRQRLSYYPNLSDSGEFR
                     AVFDAGLVLKVTDRWSATITLNDRYQSNPLPGLKKNDLLFVTGLQYVFNP"
     gene            1009..2406
                     /locus_tag="AC158Q_13305"
     CDS             1009..2406
                     /locus_tag="AC158Q_13305"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3340791.1"
                     /translation="MNDPRPINAPTRELAGWISRLGYADLPERTREVVRILLLDTLGC
                     GVYGYATPWARMLLQWVRANPPVKGEATVWGETAPSLRASDAALVNGTSVHAFELDDY
                     HQAKLHPGAVVIPAAIAMAEKLGSTGERLVTAIAAGYEVMIRSSLALNPSATRLRGWH
                     LTGVCGPFGAAAACASLMGLNEEQTAWALGLAGTQGSGLWAFNADGTMSKRFHAGRAA
                     QSGVMAAELAAIGFTGPTQIYEFHDGGVLKAFSDASDPAPLTRDLGRVFHLDATSIKP
                     YSCCGSTHSYVDAALELRRKLGARWDPKRPVRVGMAKVVDVQCGFDYVPGSALNAQMS
                     LRYVVAAALMGGQALPPQFSDAKIADPDLTGLAGRIHCEHDPALDKLYPARFAAWVAA
                     QDQGRWVRVDVLDPLGSTANPVDARGVIAKFRGINPQLPVDAIAGAAYDIERHSVREL
                     LGLLAGTQGKQLKTA"
     gene            complement(2459..3292)
                     /locus_tag="AC158Q_13310"
     CDS             complement(2459..3292)
                     /locus_tag="AC158Q_13310"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3340792.1"
                     /translation="MEAIVPGNVVTLSGLVRRITAPNPGVMTGPGTNAYILGTGDLAL
                     IDPGPESAPHLSAMLAAVGKRLKWILCTHTHLDHSPGARALKAATGAQLLGFGNVPGD
                     GRQDTGFAPDRELHEGDVLDCGAFRLRAVHTPGHASNHLCYLLEGERWLFTGDHVMQG
                     STVVISPPDGDMIEYLRSLGKLLEFDLAALAPGHGHVIDKPHDEVRRLLAHRLKREQK
                     VVSAFGRRNPATLDDLLPLVYDDVSPRIYPVARRSLHAHLLKLKHEGRAIEEDDRWAL
                     K"
     gene            complement(3280..>4071)
                     /locus_tag="AC158Q_13315"
     CDS             complement(3280..>4071)
                     /locus_tag="AC158Q_13315"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR3340793.1"
                     /translation="AATVTLVRDAPRGLEVLMLQRSHSLKFMPGVYVFPGGALDPADS
                     SPEMHALCAGLEDGPASRALGIERGGLAYWVAAIREAFEEAGILLAYDAGGGIVALNG
                     EGAERYRAHRRTLDERHGDFGAIVRGEGLRLATDRLTYFGHWITPVGAPRRYDTRFFL
                     AVAPERQEARHDNHETIAHVWMRPQEALELCAKETLNMRFPTIRTLEQFTQCATAADL
                     SAALSLNRDVRPLLPRISRDGRSLLPGDPGYEEAGAADGRGAWKP"
BASE COUNT          684 a         1407 c         1340 g          640 t
ORIGIN      
        1 cgtcaccgcc gcgtccggca ataccgactc cgttacctac gcggtcaacg gggatgcggt
       61 caggcacacc gacaaggaca agataaacgg ctatctgcag agcacgtacg gacgccgcga
      121 agtcaacggc attaccgagc gctcctcgga cctgatccgc gccggcacga agtacgaccg
      181 caacatcaac gaccgcacct tcggcttcgg cgcgctcgac ctggagcgcg accagctcat
      241 caatctcgac ctgagaagcg ttgtggcagg cggcctcggt taccacgtga taaaacgcga
      301 aggactcacg ttcgacgtct ccagcggccc ggcttacaac cgcgagagat actcgacgga
      361 tacgcgcgaa ttcatggaat ggctgttcgc cgaggaatcc atacatgccc tgaccccgac
      421 tatctcgttc cggcagcgcc tttcctacta ccccaacctc agtgactcgg gggaattccg
      481 ggcggttttc gacgccggtc tcgtgctcaa ggtaacggac cgctggagcg ccaccatcac
      541 gctcaacgac cgctaccaga gcaatccgtt gcccggcctg aagaaaaacg acctgttgtt
      601 cgtgacgggg ctgcagtacg tgttcaaccc ctgacgaatg cggacggctt tgccgccgct
      661 tcgtctccgc gcgatcgaac ggacgcgagg caccgctcat cgcgcggccc tgctcataag
      721 gattggggca gtcacaccgc cgttttatct ctgcgcgatc gagcggacgc ccgggttacg
      781 ggagccacag cccaggaact ctgatcgccg cccatcgcgc gacaatcggt ggactacttt
      841 tcacctttct atcagcgacc cggctcatgc aggggacggc cgtgccgccg cttcgtctcc
      901 gcgcgatcga acggacgcga ggcaccgctc atcgcgcggt acagaatgtg gaattcgcgc
      961 catgggaacc gcgctgggat accataacgg cactggagga ggcagcccat gaacgatccc
     1021 cgccccatca acgcaccgac gcgcgagctc gccggctgga tttcgcgcct tggttacgcc
     1081 gacctgccgg agcgcaccag ggaggtggtc cgcatactcc tgctcgacac gctgggttgc
     1141 ggcgtatacg gctacgcgac gccgtgggca agaatgctgc tgcaatgggt ccgcgcgaac
     1201 cccccggtca agggcgaggc gacggtatgg ggcgagacgg cgccatcgct gcgcgcatcc
     1261 gacgcggcgc tggtgaacgg gacttccgtt cacgccttcg agctcgacga ctaccaccag
     1321 gccaagctcc atcccggcgc ggtcgtgatt cccgcggcga tcgccatggc ggagaaactc
     1381 ggctccaccg gcgagcggct cgtgaccgcc atcgcggcag gttacgaagt catgatccgg
     1441 tcgagcctgg cattgaaccc ctccgccacg cgcctgcgcg gctggcacct gaccggcgtc
     1501 tgcgggccgt tcggcgccgc cgcggcgtgc gcttctctga tgggcctcaa tgaagagcag
     1561 accgcctggg cgctggggct cgccggcacg cagggctcgg ggctgtgggc gttcaacgcc
     1621 gacggcacca tgagcaagcg cttccatgcg gggcgggcgg cgcagtccgg ggtgatggcc
     1681 gcggaactcg ccgcgatcgg cttcaccggc ccgacacaaa tctacgaatt ccacgacggc
     1741 ggcgtgctga aggccttctc cgacgcctcc gatcccgcac cgctcacgcg cgatctcggg
     1801 cgcgtctttc atctcgacgc cacttcgatc aagccctact cctgctgcgg cagcacgcac
     1861 tcttacgtgg atgccgccct cgagctgcgc cgcaagctcg gcgcccggtg ggacccgaag
     1921 cggcccgtgc gcgtgggcat ggcgaaagtg gtggacgtgc agtgcggctt cgattacgtg
     1981 ccgggcagcg ccttgaacgc ccagatgagc ctgcgctacg tagtcgccgc ggcgctgatg
     2041 gggggccagg cgctgccgcc gcagttcagc gacgcgaaaa tcgccgaccc cgacctgacc
     2101 gggctcgccg ggcgcattca ctgtgaacac gaccccgcgc tcgacaagct ctacccggcg
     2161 cgcttcgcgg cctgggtcgc ggcgcaagac cagggccggt gggtgcgcgt ggacgtgctc
     2221 gacccgctcg gctccaccgc caatccggtg gacgcgcggg gcgtaatcgc gaaattccgc
     2281 ggcatcaatc cgcaactgcc cgtggacgcc atcgccgggg cggcatacga catcgagcgt
     2341 cattcggtgc gggagttgct cgggctgctc gccgggaccc aaggaaaaca gcttaaaacc
     2401 gcgtgacccg acgaatcacg aatcacgata attagacgcc cggtccgccc gggcgttttt
     2461 acttgagcgc ccagcgatca tcctcctcga tcgccctgcc ctcgtgcttg agcttcagca
     2521 ggtgggcgtg cagcgagcgg cgggcgacgg ggtagatgcg cggcgagaca tcgtcgtaaa
     2581 ccagcggcag caggtcgtcc agcgtggccg ggttcctgcg cccgaaagcg gataccacct
     2641 tctgctcgcg cttcaggcga tgcgcgagca ggcgccgcac ttcgtcgtgc ggcttatcga
     2701 tgacgtgccc gtggccgggc gcgagggcgg cgaggtcgaa ctcaagcagc tttcccagcg
     2761 accgcagata ttcgatcatg tccccgtcgg gcggcgagat cacgaccgtc gagccctgca
     2821 tgacgtgatc gccggtgaac agccagcgct ccccctccag caggtagcac aggtggttcg
     2881 aagcgtggcc cggcgtgtgc acggcgcgca gacggaaagc gccgcagtcg agcacatcgc
     2941 cttcgtgcag ttcgcggtcg ggcgcgaaac cggtgtcctg gcggccgtcg ccggggacat
     3001 tcccgaaacc cagcagttgc gcgccggtcg ccgccttgag cgcccgcgcg cccggtgaat
     3061 ggtcgagatg ggtgtgcgtg cagagtatcc acttcaggcg cttgcccacg gccgcgagca
     3121 tcgccgagag gtgcggcgcg ctctcgggtc ccgggtcgat cagcgcgaga tcgccggtgc
     3181 ccaggatata ggcattggtc ccaggaccgg tcatcacgcc gggattgggc gcggtgatgc
     3241 gccggacgag ccccgacagg gtcacgacgt tgccgggcac tatggcttcc aagcccctct
     3301 cccatccgcc gcgccggcct cctcgtagcc gggatcgccg ggcaacaggc tccgcccgtc
     3361 gcgcgagatt cgcgggagca gcggccgcac gtcccggttc agggacaggg cggcgctcaa
     3421 atcggcggcc gtggcgcact gcgtgaactg ctcgagcgtc ctgatggtcg ggaagcgcat
     3481 gttgagcgtc tccttcgcgc acagttcgag ggcttcctgc ggccgcatcc agacgtgggc
     3541 aatggtctcg tgattgtcgt gccttgcctc ctgccgctcc ggcgcgaccg cgagaaagaa
     3601 gcgcgtgtca tagcgccgcg gcgcgccgac cggggtaatc cagtgcccga agtacgtcag
     3661 ccggtccgtt gcgagacgca ggccctcgcc gcgcacgatc gcgccgaaat ccccgtgccg
     3721 ctcgtcgagc gtgcggcggt gggcgcgata gcgctcggcc ccctccccgt tcaaggcgac
     3781 gatgccgccg ccggcgtcat acgcaagcag gatgccggcc tcctcgaacg cttcgcggat
     3841 cgccgcaacc cagtacgcca gcccgccccg ttcaattccg agcgcccggc tcgccggtcc
     3901 gtcctcgagg ccggcgcaca gcgcgtgcat ttccggggaa ctgtcggcgg ggtcgagcgc
     3961 gccaccggga aagacgtaga cgcccggcat gaacttgagg ctgtgactgc gctgcagcat
     4021 caggacttcg agaccgcgcg gcgcatcgcg cacgagcgtg acggtggctg c
//
LOCUS       DBNGSG010000502        10888 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1522645, whole genome shotgun sequence.
ACCESSION   DBNGSG010000502 DBNGSG010000000
VERSION     DBNGSG010000502.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 10888)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10888)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10888
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1522645"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="AC158Q_13320"
     CDS             <1..714
                     /locus_tag="AC158Q_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgH/BhsA/McbA family protein"
                     /protein_id="HIR3340794.1"
                     /translation="RLMPLARISGLFILLLTAGCAAMDCTPQGAPPGAGAAQEKTPAG
                     ESQPAAPKQGPAIATQPVHEAPKRDRPMAAEPAPPVAASANRSAAQPQAPTAQPPVKV
                     AEAPAPSAPASKKENAAPAAAKPAPKKEGAAPVTAKPAPMDLTLLEKRLKETPAIGVF
                     TKLTLKNQVDDLLDQFKAYYQGRAKVTLAALRQPYDQLILKVLSLLQDSDPPLARAIM
                     ESREAIWGILSDPEKFKNL"
     gene            736..984
                     /locus_tag="AC158Q_13325"
     CDS             736..984
                     /locus_tag="AC158Q_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340795.1"
                     /translation="MSKSMKIRALIAVLPALLAAPSFAADTLAKDLTSTIALLGLPCG
                     QVVSAQRLKDNDYIATCKDKNRYRVFINAEGRVVAQKQ"
     gene            1055..1471
                     /locus_tag="AC158Q_13330"
     CDS             1055..1471
                     /locus_tag="AC158Q_13330"
                     /inference="COORDINATES: protein motif:HMM:NF047765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LIC_13387 family protein"
                     /protein_id="HIR3340796.1"
                     /translation="MAECLLVAGGAIYLLLGSIHALYTLLDLHRPRRIVPADAALIDR
                     MRGTTVRLSRGGSTMWDAWMGFNLSHSLGAILFGLGCIAAGIGLWGLVPTKASLLIPV
                     AVSTLYLVLALRFWFKVPAFGIAAATILLATAWLLR"
     gene            1713..1913
                     /locus_tag="AC158Q_13335"
     CDS             1713..1913
                     /locus_tag="AC158Q_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340797.1"
                     /translation="MLSEELDSLRQLAGEAILEHIPSRHGERLVHCGYASLRGGALMI
                     TTMGRARLAFEITRASWLGAPG"
     gene            1932..2114
                     /locus_tag="AC158Q_13340"
     CDS             1932..2114
                     /locus_tag="AC158Q_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340798.1"
                     /translation="MDQAVGFDELRWLQELTLKTQKLTVPETIGNRLLTRGLIERTPG
                     GYSLTARGRIALAKLG"
     gene            2152..2421
                     /locus_tag="AC158Q_13345"
     CDS             2152..2421
                     /locus_tag="AC158Q_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HIR3340799.1"
                     /translation="MNIATWILAGGIIGWIGFSLLKFNLKRGMIVSILIGMAGGYLGG
                     SLLAPMLSAALTTHGDFSPLSLFTAFACAAGFVIVSDMFYKRFGM"
     gene            2491..2706
                     /locus_tag="AC158Q_13350"
     CDS             2491..2706
                     /locus_tag="AC158Q_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340800.1"
                     /translation="MKAQLMHVDNGAGRVVVGPKKLKSCYVRIVSLKDGSGRIERFDL
                     VSRTWLEAPATITFSEVWSAPSASALL"
     gene            2757..2948
                     /locus_tag="AC158Q_13355"
     CDS             2757..2948
                     /locus_tag="AC158Q_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340801.1"
                     /translation="MNNIWRFYVDQDRLWRWQRLAADRSLISESRAAYKEYESCLANA
                     KIEGYVFHPSQDKLKSLAH"
     gene            3117..3323
                     /locus_tag="AC158Q_13360"
     CDS             3117..3323
                     /locus_tag="AC158Q_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR3340802.1"
                     /translation="MPTGTVKFFNSTKGFGFIQPDDGSKDVFVHISAVESAGLGSLTE
                     NQKVSFEVQQGNNGKSSAVNLKSA"
     gene            3438..3914
                     /locus_tag="AC158Q_13365"
     CDS             3438..3914
                     /locus_tag="AC158Q_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HIR3340803.1"
                     /translation="MHIWVDADACPVVVKEILYRAAERLQLPMTLVANKPLRTPSSRF
                     IRTVQVARGLDVADNEIARCLEVGDLVVTADIPLAADIIARGGHALNPRGEFYTSENV
                     REHLSMRDFMEALRGGGVQTGGPQPLDQADRKRFADQLDRFLSRSAQTLARSAAGG"
     gene            3974..4969
                     /locus_tag="AC158Q_13370"
     CDS             3974..4969
                     /locus_tag="AC158Q_13370"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3340804.1"
                     /translation="MKNKAGVGLRNGIVTGLAAALAVAAPHGAAQPAPAYPTKPVRLI
                     VSSGAGGGLDFVARQVAAPLGEYLGQTVVVDNRAGASGSIAAELASQAAPDGYTLMFL
                     SASLVVYGIVNKTRYDLYKDFAPVSQVAAAPYMLAVTPSLPVKNVKELIAHAKSASSK
                     LNYASTGQASFAHLATELFAHLTGTQLVHIPYKGVGAAVPDMFSGRVHMAFLSMGSLY
                     GHVRAGRLRGLAVATPDRVKMAPELPTMIESGVPGYVVTQWHGMAAPRGTPRPVIDRV
                     QREIAKCVKRPEVASRLALDGTEGIASTPQQFAAFLGSEREQWAKAAKLANIRAN"
     gene            complement(5054..5410)
                     /locus_tag="AC158Q_13375"
     CDS             complement(5054..5410)
                     /locus_tag="AC158Q_13375"
                     /inference="COORDINATES: protein motif:HMM:NF021221.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HIR3340805.1"
                     /translation="MSEKPQAKEEQNWAMICHLAALSGFVIPLGNVLGPLVVWLMKKD
                     TMPLVDVHGKEALNFQITVTIAMVISIVLMFVLIGILLVFVVGIGALVLTILAAVKVS
                     NGQLDYKYPFALRLIK"
     gene            complement(5489..6463)
                     /locus_tag="AC158Q_13380"
     CDS             complement(5489..6463)
                     /locus_tag="AC158Q_13380"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3340806.1"
                     /translation="MKRARYLAAIVLAAASAGALAQAWSPQKNVEIVAGSAPGGSNDK
                     TARAMERILLAGKLVPVTLTVVNKPGGGGSIAYTYVSQRPGDAHYLYIASSGLLSNHI
                     VGASSLSHADFTPIALLYEDAAVFAVRAESPIRTGKDLVDRLKKDPRSVTVGFANAFG
                     SSRHMAAALLMKTLGANARDLKPVVFKGSAEAITAMLGGHIDVVVAGAVNAIVHVAGG
                     QMRVIGVAAPQRLGGPLAVAPTWKEQGVDLVYGNWRAIFAPRGLAPAQVAYWETTLRR
                     MTASPEWKADLEKSHWTEHFVTGAPLHKEIEQEYAWLKTTLVELGLAR"
     gene            complement(6492..6830)
                     /locus_tag="AC158Q_13385"
     CDS             complement(6492..6830)
                     /locus_tag="AC158Q_13385"
                     /inference="COORDINATES: protein motif:HMM:NF014376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3340807.1"
                     /translation="MPFFKLDEMPGSVIAPGHSTAHGPTITGKELEIGYYTEPRGTGA
                     KPHHHKSEQIILVLSGRLQMRVGDELREIGPGEAALILSDQEHEQKALEDGTRFISFK
                     TAAGAQPAGE"
     gene            6925..7719
                     /locus_tag="AC158Q_13390"
     CDS             6925..7719
                     /locus_tag="AC158Q_13390"
                     /inference="COORDINATES: protein motif:HMM:NF013847.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340808.1"
                     /translation="MLFPTMLVGSYPQPEWLIDREKLAGRFPPRVRAKELWRIPEPWL
                     AQAQDDATVMAIRAQEDAGLDIITDGEIRRESYSNRFATALEGVDIDNPGTALDRSGH
                     PNPVPRIVGRIRRRHPVEVEDLKFLKRHTSRMVKITVPGPFTMSQQAQNDYYKSDEEA
                     AMDYATAVNEEIRDLFAAGADIVQIDEPYMQARPEKARQYGLKALVRALDGIEGTTAV
                     HICFGYAAVIHERPSGYSFLPELAGCGCKQVSVETAQSSLDCAVRE"
     gene            7738..7947
                     /locus_tag="AC158Q_13395"
     CDS             7738..7947
                     /locus_tag="AC158Q_13395"
                     /inference="COORDINATES: protein motif:HMM:NF013847.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340809.1"
                     /translation="MVGSIDLSTMTVETPETVVARVKRALPYVRPENVIIAPDCGMKY
                     LTRDAAFGKMRAMAEAAKILRREFK"
     gene            8041..8847
                     /locus_tag="AC158Q_13400"
     CDS             8041..8847
                     /locus_tag="AC158Q_13400"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3340810.1"
                     /translation="MLDLKSKVAFISGAGTVGPGWGNGKATAVLFARQGAKVFGTDIN
                     LEAAEETRAIIEKEGGDCAVRRCDMTASVDVKAAVDACIERYGRIDILVNNVGGSAPG
                     GPVDMPEEVWDGQLDHNLKTAFLGCKHVLPVMEKQGGGAVVNIASVAGFSHQVGGRVH
                     VAYSTAKSGLLGFTRSTAIAYVRKGIRCNLVIVGTMHTPLVEARLMKQLGAKEAADLV
                     AKRHAAVPIGRMGDAWDVANAVLFLASDEARYITATHLTVDGGLTAARPG"
     gene            8911..9705
                     /locus_tag="AC158Q_13405"
     CDS             8911..9705
                     /locus_tag="AC158Q_13405"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3340811.1"
                     /translation="MAARFVMAILALLWSLGALAQGESIGVVIMHGKGGSPTGLVAEL
                     ARTLGDRGYLVANLDMPWSGSRHYDVPVSRAVEEVEAAVAGLRGKGAKKVFVAGHSQG
                     GAFATHLAGKLAVDGVIVIAPGGNVDHFFFRDKIRGSLARARQLVADGKGGEPATLDD
                     FEGGRGLFPVVTAPAVYVTWFDPEGAMNMARAARAANPATPILWIVPSRELPGLRKTN
                     IPMFRSLPAHPLTRYYEPDSDHRGTPTASADEIARWTREVAAARWR"
     gene            complement(9932..>10888)
                     /locus_tag="AC158Q_13410"
     CDS             complement(9932..>10888)
                     /locus_tag="AC158Q_13410"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3340812.1"
                     /translation="LSLLAISAPMPAGPTLAADYPDRPIRWIVPFPPGGSNDILARYL
                     GVKLSERLGQQVVIDNRAGANGIIGAELAAQSTADGYNLLMVSTSWILNAAVRTFPLP
                     YDIEKSFDPITTIGSAPNSIVVNPKGPYQSIGALVSAAKAQPGSINYAHTGVGGFNHF
                     GGELLKKIAGIRMTPVPYKGGGPAMVDVMAGNIPVMFSSVTQALPHVRNNRLKFLAVG
                     AAKRSPAVPEVPTVAESGFPGYEVAVWWGIVVPAGVPRPVLAKLNRDFAAVLQAAETK
                     QRLAAEAAEPEILSPAAFRKKISFERKKWAELAKQAGIQMSR"
BASE COUNT         1882 a         3506 c         3613 g         1887 t
ORIGIN      
        1 cgcttgatgc cactcgctcg tatctccgga cttttcatcc tgctcctgac ggccggttgc
       61 gccgcgatgg attgcacgcc gcagggcgcg ccgcccgggg ccggggctgc gcaggaaaag
      121 acacccgctg gcgaatcgca gccggcggcg ccgaagcagg gaccggccat cgcaacccag
      181 ccggtgcacg aggccccgaa gcgggaccgg cccatggcgg ccgaaccggc gccgcctgtc
      241 gctgcttccg cgaaccgaag tgccgcccag ccgcaagcgc caacagcgca accgccggtc
      301 aaggttgccg aggctcccgc gccgagtgcg ccggcgtcga aaaaggagaa tgctgcgccg
      361 gcagcggcga agccggcgcc gaagaaggag ggtgccgcgc cagttacggc aaaaccggcg
      421 ccgatggatc tgacattgct ggagaaacgg ctcaaggaga caccggccat cggggtattc
      481 accaaattga cgctcaagaa ccaggtggat gacctgctgg accagttcaa ggcgtactac
      541 cagggacgcg ccaaggtcac cctggccgcg ctgcgccagc cgtacgatca actgatcctg
      601 aaggtgctct cgctcctgca ggacagcgac cctccgctgg cacgcgcgat catggaatcg
      661 cgcgaggcga tctggggcat actttccgat ccggagaaat tcaagaatct ctgaccttct
      721 gaagagaggc acacgatgag caaatcaatg aaaatccggg ccctgatcgc cgtgctgccg
      781 gcgctcctgg ccgccccgtc gtttgcagcg gacacgctcg ccaaggacct cacctccacg
      841 atcgcgctgc ttggattgcc ctgcggacag gtcgtcagcg cccagcggct gaaggacaac
      901 gactacattg ccacctgcaa ggacaagaac cgctaccggg ttttcatcaa cgccgagggc
      961 cgggtggtcg cgcagaagca atgagccgcg ccagcctgac ggccgaggct ttaccacttc
     1021 gtcaccttgt cgcttcatca accccggcgg gccaatggct gaatgcttgt tggtagccgg
     1081 cggcgcgatt tacctgctgt tgggatccat ccacgcgctc tacaccttgc tcgacctgca
     1141 ccggccccgc cgcatagtgc ccgccgacgc cgcattgata gaccgcatgc gtgggaccac
     1201 cgtacgcctg tcgcggggcg ggtcaaccat gtgggatgca tggatgggct tcaacctgag
     1261 tcatagcctg ggcgccatcc tgttcgggct gggttgcatc gccgcaggca tcgggctttg
     1321 ggggcttgtc ccgacgaaag cttcgttgtt gatccccgtg gcggtcagca ccctctatct
     1381 cgtgctggcg ctgcgcttct ggttcaaagt gcccgcattc ggcatcgccg ccgcaacgat
     1441 actgctggcc accgcgtggt tgctgcgcta gaggctgttt cgatcagatt ctgatgcctc
     1501 atttcgctcc cggtgagcgg aaatagggat tgccctttgc gcctgaaccg cgtataacaa
     1561 atcatgcgtt tagtttcatc tccttgggca cgtttactgt tcctgccggg ccgattctgt
     1621 atccgcaacc atcagggcct gggattaaac atacccgggc ttgcccccgt cccgcgttcc
     1681 gcaaaggatt catggccgcc gtccgcacag ccttgctgtc agaagaactg gactcgctgc
     1741 gtcagctcgc cggcgaggcg atcctcgagc acatccccag ccggcacggc gagaggctgg
     1801 tgcattgcgg ctacgcgagt ctcaggggcg gcgcgctgat gatcaccacc atgggacgcg
     1861 ccagactggc gtttgaaatc acccgcgcga gctggctggg cgcgccgggc tgaacggaaa
     1921 gggcgcacgc gatggatcag gcagtcgggt ttgacgaatt gcgctggctc caggagctga
     1981 cgctgaaaac gcagaaactg acggtgcccg agactatcgg caaccggttg ctgacgcgcg
     2041 ggttgatcga gcggacgccc ggcggctact cgctcacggc aagaggccgg atcgcgctgg
     2101 ccaagctggg ctgaccgccc ggcaccgccc ccggttcctc gcaaggcaaa aatgaacatc
     2161 gcgacctgga tcctggcagg cggcattatc ggctggatcg gattttccct gctcaagttc
     2221 aacctgaagc gcggcatgat cgtctcgatc ctcatcggca tggccggggg gtacctgggc
     2281 ggaagcctgc tcgcgccgat gctgagcgca gccttgacca cgcatggcga cttcagcccg
     2341 ctctcgctgt tcaccgcgtt cgcgtgcgcc gcgggattcg tgatcgtcag cgacatgttt
     2401 tacaagcgtt tcggcatgtg accatgcgcg gcttcgcgag accgccgatg gaagcacgca
     2461 gagccggtga tgaccgggga ggcagctgag gtgaaagcgc agctcatgca cgtggacaat
     2521 ggggcgggca gggtggtggt ggggccgaag aagctgaaga gctgctatgt ccgcatcgtc
     2581 tccctgaaag acggatccgg gcgcatcgag cgattcgatc tcgtttccag aacctggctc
     2641 gaggctcccg cgaccatcac tttcagcgag gtctggagcg cgccttcggc gtcggcattg
     2701 ctgtgagcac ggtcatgaac ccggtatcgc gacagggtcc agtgaggacg gcgcggatga
     2761 acaacatatg gcgcttttac gttgaccagg accgcctgtg gcggtggcag cggcttgcgg
     2821 cggaccgttc gctgatttcc gagtcccggg cggcctacaa ggagtacgaa agctgcctgg
     2881 ccaacgccaa aatcgagggc tacgtattcc atccctcgca ggacaagctc aagtcccttg
     2941 cgcattgagc gcgcgcgccc ctagccctgc cgccggtttc ggcccataat gccggggtcg
     3001 aattcgcttt gggtctgtcc gcggtctcct ttccggctgg cttttcggtc cctcccatag
     3061 attatttgat gcctcgcgca ctatcgcgtg aagcaacgcc tcatctggag ggcatcatgc
     3121 ctaccggcac cgtaaaattt ttcaacagca ccaagggttt tggcttcatc caaccggatg
     3181 acggctcaaa agacgttttc gtgcacatct ccgcggtcga aagcgccggt ctgggctcgc
     3241 tgaccgagaa ccagaaggtc tcgttcgaag tgcagcaggg caacaacggc aagtcctcgg
     3301 cggtcaatct caagtccgcc tgagcgtttc ccgcgccggg tttctgtccg gcgcgcactt
     3361 gcgaaacccc gcctcggcgg ggtttcgttt gcagccgccg cagcgggcct ttttacaacc
     3421 tttgtccggg agccggaatg cacatctggg tcgatgccga tgcctgcccg gtggtcgtca
     3481 aggaaatact gtaccgcgcg gcggaacggc tgcagttgcc gatgacgctg gtggcgaata
     3541 agccgctgcg cacgccgtct tcgcgcttca tccggacggt ccaggttgcc cgcgggctcg
     3601 acgtcgccga caacgagatc gcccggtgcc tcgaggtagg agacctggtc gtcacggccg
     3661 atattccgct tgccgccgat atcattgcgc gcggcggcca tgcgctcaat ccgcgcggcg
     3721 agttctacac ttccgagaat gtccgcgagc acctgtcgat gcgcgatttc atggaggcgc
     3781 tgcgcggcgg cggcgtgcag accggcggcc cgcagccgct cgaccaggcc gaccgcaagc
     3841 gcttcgcgga ccagctggac cgctttctca gccgttcggc gcagacgctt gcccgctcgg
     3901 cggccggggg ctgaggcggg cggtgagcgg ggtctgctaa tatttgcaca gtggatattg
     3961 gcgaggaggc gatatgaaaa acaaagccgg agtgggtttg cgcaatggca tcgtcacggg
     4021 actggccgcg gcgctcgccg tcgcggcacc gcatggcgcc gcgcaacccg cgccggcata
     4081 cccgaccaag ccggtccggc tgatcgtctc gtcgggggcg ggcggcgggc tggatttcgt
     4141 cgcgcgccag gtcgccgcgc cgcttggcga atacctgggg caaacggtgg tcgtggataa
     4201 ccgggcgggc gcgagcggca gcattgccgc ggagcttgcc tcgcaggcgg cgccggatgg
     4261 ctacaccctg atgttcctga gcgcgagcct cgtggtgtac ggcatcgtca acaagacgcg
     4321 ctacgacctc tacaaggatt tcgccccggt ttcccaggtt gcggccgcgc cctacatgct
     4381 cgccgtgaca ccctcgctgc cggtgaagaa cgtgaaggag ctgatcgccc acgcaaaatc
     4441 ggcttcatcg aagctgaact atgcctcgac ggggcaggct tccttcgcgc acctcgcgac
     4501 cgagctgttc gcgcatctga ccgggacgca gctggtgcac atcccgtaca aaggggtcgg
     4561 cgcggcggtg cccgacatgt tctcgggccg cgtgcacatg gcctttctga gcatgggatc
     4621 cctgtatggc cacgttcgcg ccgggcggct caggggtctc gcggtggcca ccccggaccg
     4681 tgtgaagatg gcgccggagc tgccgacgat gatcgagtcg ggcgtgccgg gttacgtggt
     4741 cacccagtgg catggcatgg cggctccccg cggcacgcca cggccggtca tcgaccgcgt
     4801 gcagcgggag atcgcgaagt gcgtgaagcg gccggaggtt gcgtcgcggc tggcgctcga
     4861 cggcaccgag ggtatcgcga gcacgccgca gcagtttgcc gcgttcctcg ggagcgagcg
     4921 cgagcagtgg gcgaaggcgg cgaagctcgc caacatccgc gccaactagc ccgacgcgca
     4981 aggggaaccg ggtaacgggc aacaggcctt cgacgcgggt cctgtctccg catcacccat
     5041 caccggggta aagttacttg atcagccgga gcgcgaacgg gtacttgtag tcgagctggc
     5101 cgttcgaaac cttgaccgcg gcaagaatgg tcaggacgag cgcgccgata ccgaccacga
     5161 acaccagcag gatcccgatc aggacgaaca tcagcacgat gctgatgacc atcgcgatcg
     5221 tcaccgtgat ctggaaattg agcgcttcct tgccatgcac atccaccagc ggcatggtgt
     5281 ccttcttcat cagccatacc accagcgggc ccagcacgtt gcccagcggt atgacaaagc
     5341 cggacagcgc ggcgaggtgg cagatcatcg cccagttctg ttcttccttg gcttgcggtt
     5401 tttcgctcat tttcgtctcc ctgtgttggt gctctctgcg caacgcctgc aaagaataca
     5461 ctacccccat ccacccatag cctgccgctt acctggccag ccccagttcc accagcgtcg
     5521 tcttcagcca ggcgtattcc tgctcgattt ccttgtgcag cggcgcgccg gtcacgaaat
     5581 gctcggtcca gtggctcttt tcgaggtccg ctttccattc gggcgacgca gtcatcctgc
     5641 gcagggtggt ttcccagtac gcgacctggg cgggcgcgag ccccctgggc gcgaagatcg
     5701 cgcgccagtt gccgtagacg aggtccacgc cctgttcctt ccacgtgggg gcgaccgcga
     5761 gcggcccgcc caggcgctgc ggcgcggcga cgccgatcac ccgcatctgc ccgccggcga
     5821 cgtggacgat cgcgttgacg gcgccggcga cgacgacgtc gatgtggccc cccaacatgg
     5881 cggtaatcgc ttcggccgaa cccttgaaca cgaccggctt gagatcgcgc gcgttggcgc
     5941 cgagcgtctt catgagcagc gcggcggcca tgtggcggct gctgccgaac gcgttggcga
     6001 aacccaccgt cacggagcgc gggtctttct tcagccggtc gacgaggtcc ttgccggtcc
     6061 tgatcggcga ctcggccctg acggcgaaca ccgccgcatc ctcgtagagg agagcgatgg
     6121 gcgtgaagtc ggcatggctc aggctgctcg cccccacgat gtggttggaa agcagcccgc
     6181 tgctggcgat gtacaggtaa tgcgcgtcgc cggggcgctg gctgacgtag gtgtaggcga
     6241 tgctgccgcc gccgcccggc ttgttcacga cggtgagcgt gaccgggacc agcttgcccg
     6301 cgagcaggat gcgctccatc gcccgcgcgg tcttgtcgtt gctgccgccg ggcgcggagc
     6361 ccgccacgat ctcgacgttc ttctgcggcg accacgcttg cgcgagcgcc ccggcgctcg
     6421 ccgccgcgag gactattgcc gccaggtaac gggcccgttt catgaccgct tccttgtcag
     6481 gttgatggcg tttattcgcc tgccggctgc gcgccggcgg ccgtcttgaa actgatgaaa
     6541 cgcgtgccgt cttccagcgc cttttgctcg tgctcctggt cgctcaggat cagggcggct
     6601 tccccggggc cgatctcgcg cagctcatcg ccgacgcgca tttgcagccg gccggacagc
     6661 accaggatga tttgctcgct cttgtggtga tgaggcttcg cgccggttcc cctgggctcg
     6721 gtgtagtagc cgatttccag ctccttgccg gtgatggtgg ggccgtgggc agtggaatgg
     6781 cccggcgcta tgaccgagcc cggcatttcg tccagtttga aaaagggcat ggctattccg
     6841 atccgggaga tcgtttggca gcgggaaatc gggaacccgg ctagactact cttgagtccc
     6901 gcacgtgaca aatgaggtat cgacatgctg tttcccacca tgctcgtcgg cagctacccc
     6961 cagccggagt ggctgatcga ccgcgaaaag ctggccgggc gcttcccgcc gcgcgtgcgc
     7021 gcgaaggagt tgtggcgcat accggagccc tggctcgcgc aggcccagga cgacgccacg
     7081 gtcatggcga tcagggcgca ggaggacgcc ggcctcgaca tcatcaccga cggcgagatc
     7141 cggcgcgaga gctactcgaa ccgcttcgcc accgcgctcg agggggtgga catcgacaac
     7201 ccgggcaccg cgctcgaccg ctcgggccat cccaacccgg tgccgcgcat cgtcggcagg
     7261 atccgccgca ggcacccggt cgaggtcgag gacctgaagt tcctcaagcg ccacacgagc
     7321 cggatggtga agatcaccgt gccggggccg ttcacgatgt cgcagcaggc gcagaacgac
     7381 tattacaagt cggacgagga agcggcgatg gactatgcaa ccgccgtgaa cgaggagatc
     7441 cgcgacctct tcgcggccgg agccgatatc gtgcagatcg acgagcccta catgcaggcg
     7501 cggccggaga aggcgcgcca gtacgggctg aaggcgctgg tgcgcgcgct ggacggcatc
     7561 gagggcacga ccgccgtgca catctgcttc ggctacgccg ccgtcatcca cgagcggcct
     7621 tcgggctatt ccttcctgcc ggaactggcc ggatgcggct gcaagcaggt ctcggtcgag
     7681 accgcgcaat ccagtctcga ctgcgcggtg cgggagtagc tggaaggcaa gaagatcatg
     7741 gtggggtcga tcgacctgag caccatgacc gtcgagacgc cggagaccgt ggtggcgcgc
     7801 gtgaagcgcg cgctgcctta cgtcaggccg gagaacgtga tcatcgcgcc cgactgcggc
     7861 atgaagtacc tgacgcgcga cgccgccttc ggcaagatgc gcgcgatggc ggaagcggcg
     7921 aagatcctgc gccgcgagtt caagtgatcg gcgacgaggg taccgaaccc ggtaaaatcc
     7981 cgccacgccg tcacgggcta cgataagcaa aaaacgaaca acgaaaaacc agtaacgcaa
     8041 atgctggatc tcaagagcaa ggttgccttt atttcaggcg ccggcacggt cggccccggc
     8101 tggggcaacg gcaaggcgac cgcggtgctg ttcgcgcgcc agggcgcgaa ggtgttcggc
     8161 accgacatca acctggaggc ggccgaggaa acgcgagcga tcatcgagaa ggagggcggc
     8221 gactgcgcgg tgcgccgctg cgacatgacg gcttccgtcg acgtgaaagc cgccgtggat
     8281 gcgtgcatcg agcgctacgg ccgcatcgac atactggtga acaatgtcgg cggctcggcg
     8341 cccggcggcc cggtcgacat gcccgaggag gtgtgggacg gccagctcga ccacaacctc
     8401 aagacggctt ttctcggctg caagcacgtg ctgccggtga tggagaagca gggcggcggg
     8461 gcggtcgtga acatcgcatc cgtcgccgga ttcagccacc aggtgggcgg gcgcgtgcac
     8521 gtcgcctaca gcacggcaaa gtcgggtttg ctcggattca cccgctcgac cgccatcgcc
     8581 tacgtcagga aaggcatccg ctgcaacctg gtgatcgtcg gcacgatgca cacgccgctg
     8641 gtcgaggcgc ggctgatgaa gcagctgggc gcgaaggagg ccgccgacct cgtcgcgaag
     8701 cgtcacgcgg ccgtgccgat cgggcgcatg ggcgacgcgt gggacgtcgc gaacgcggtg
     8761 ctgttcctcg cctcggacga agcgcgctac atcaccgcca cgcatttgac ggtcgacggc
     8821 gggctgacgg ccgcacgacc gggctgaacg acagggatga gggatgaggg acgagtgatg
     8881 agtgacgggt ggcgagtaat cggagggaat atggccgcgc gattcgtgat ggcgatcctg
     8941 gcactgctct ggtcgctcgg ggcactggcg caaggcgagt cgatcggcgt cgtgatcatg
     9001 cacggcaagg gcgggtcgcc gacggggctc gtcgccgaac tggcgcggac gctgggggac
     9061 aggggttatc tcgtcgccaa tctcgatatg ccatggtccg ggagccgcca ttacgacgtt
     9121 cccgtgagcc gggccgtgga ggaagtcgag gccgcggtgg cgggcctgcg cggcaagggc
     9181 gcaaagaagg tgttcgtcgc gggtcacagc cagggcggcg cgttcgcaac ccatctcgcg
     9241 ggcaagctgg cggtggacgg cgtcatcgtc atcgccccgg gcggcaacgt cgatcacttt
     9301 ttcttccggg ataagatccg gggctcgctg gcccgcgccc gccagctcgt cgcggacggc
     9361 aagggcggcg agccggcgac cctcgacgat ttcgaaggcg gcaggggcct gtttccggtc
     9421 gttaccgctc ccgccgtcta tgtaacatgg ttcgacccgg aaggcgccat gaacatggcc
     9481 cgggcggctc gggccgcgaa tccggcgacg ccgatcctgt ggatcgttcc aagtcgggag
     9541 cttccgggcc tgcgcaaaac gaacatcccg atgtttcgat cccttccggc gcatcccctg
     9601 acccggtact acgagcccga ctccgatcat cgcggcacgc ccactgcctc ggccgacgaa
     9661 atcgcgcgct ggacgcgaga ggtggccgcc gcccggtggc gctgatttcc ccggggggca
     9721 gcgaagcgcc tggaaagaaa aaggccggac ccgtgctccg gtccggccca atgtgtcccc
     9781 cactaggaca caagctttct cctttgtttt tcctcctcct cttgttatcc cctgtttacg
     9841 ttcacctgtt accggcagct cgcttacttg accttgaacc cgggggccct cgcgatggcg
     9901 cgccgattca agcagtccgc gaaccgtcct ctcatcggct catctggatc ccggcctgct
     9961 tcgcgagttc ggcccacttc tttctctcga agctgatctt tttccggaac gcggccggcg
    10021 acaggatctc cggctcggcg gcttcggcgg caagtcgctg cttcgtttcg gcagcctgga
    10081 gaaccgccgc gaagtcgcgg ttcaatttcg ctagcacggg ccgagggacg ccggcgggca
    10141 cgacgatgcc ccaccacacc gccacctcgt agcccgggaa gccggactcg gcgacggtcg
    10201 gcacctccgg cactgccggg ctgcgcttcg ccgcgccgac cgcgaggaac ttgagccggt
    10261 tgttgcgaac gtgcggcagc gcctgggtga cggagctgaa catcaccggg atgttacccg
    10321 ccatcacgtc gaccatcgcc ggcccgccgc ccttgtaggg gactggagtc atcctgatgc
    10381 ccgcgatctt cttcagcaat tcgccgccga aatgattgaa tccgccgacg ccggtatggg
    10441 cgtagttgat cgaacccggc tgcgctttcg ccgcggaaac cagggcgccg atcgattggt
    10501 acgggccctt gggattgacc acgatcgaat tgggggcaga accgatcgtc gtgatcgggt
    10561 cgaatgattt ctcgatgtca tagggcaagg ggaaggtacg gaccgcggca ttcaggatcc
    10621 aggacgtgga aaccatgagc aggttgtagc cgtccgcggt cgactgggcg gcaagctccg
    10681 cgccgatgat gccgttcgcg cccgcgcggt tgtcgatcac gacctgctgg ccgagacgct
    10741 cggacagctt gaccccgagg tagcgggcca gaatatcgtt gctgccgccc ggcgggaatg
    10801 gaacgatcca gcgaatcggc cgatccgggt aatcggcggc gagcgtgggt cccgcgggca
    10861 tcggtgccga aatagcgagc aggctgag
//
LOCUS       DBNGSG010000503         4577 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_504425, whole genome shotgun sequence.
ACCESSION   DBNGSG010000503 DBNGSG010000000
VERSION     DBNGSG010000503.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4577)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_504425"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="AC158Q_13415"
     CDS             <1..704
                     /locus_tag="AC158Q_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HIR3340813.1"
                     /translation="ASGAEIVTVAIRRTNIGQDAGQPSLLDYLPPSKFALLPNTAGCY
                     TADDAVRTLRLARDLLDGHDLVKLEVLGDPRTLYPNVVDTLAAAKTLVRDGFKVMVYT
                     SDDPIIAKQLEEIGCVAVMPLASLIGSGMGILNPWNLQIIIESARVPVIVDAGVGTAS
                     DAAIAMELGCDGVLMNTAVAAAGNPVLMAGAMKKAVEAGRAAFLAGRMPKKLYSGSPS
                     SPTTGLVTPPAPSKG"
     gene            complement(790..4017)
                     /locus_tag="AC158Q_13420"
     CDS             complement(790..4017)
                     /locus_tag="AC158Q_13420"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR3340814.1"
                     /translation="MLAGSRGSPLAAIAGRAGLTWPAFLVLAVGLAISFAAWNTTEQR
                     VGREAEAKFQNHVAQAMGTLDRRMQDNVNLLIGLRGFFAASNRVDRDEFRLYLSGFNV
                     TQRYSGIRLMSFVRYVTPAEKAGFEESVRRDSSVDSRGYPDFAIKPAGDRADYMVVTY
                     IEPFAGNEGAFGFDVYSEPQRRASVERARDSGQVTASEPILLAADPQKQISLALRMPL
                     YRRGAPAGTLEERRAAFLGVVSSAIRVDDLVGSQLGRELGSEFDLAIHDLGFSGMDGQ
                     PAGRGRDDLVFDSSRTFGMKAVKEAGHAFLSQVMTLDVAGRHWRLEFRAPRIPAHGIS
                     SALPPLTLMGGILTSLLLFWGVWTLLVSRERALRFAEHARAMSAAEGLREQLAFIQQL
                     IEAVPQPIFFKDVEGRYLGVNKAWEGFFGIPREQFIGKSVFELYPNNLELARKHHAKD
                     QELFGRPGSQSYEAAIIAADGRVHHTIYNKATFNKFDGSAAGLIGTITDVTGLKEAEA
                     ALRQSEERFRDLTELSSDWYWEQDANYRFTQLSSKIHEISLDAEEHIGRTRWEMPILG
                     VSADQWEMHKGLLAAREPFQDFTYQRHDADGNLRVISVSGRPIFDEQGRFRGYRGTGR
                     DVTEQKQTEEKIRHMAHHDALTQLPNRTLLYDRIGQAIVQAQRNRESIALLFIDLDRF
                     KTVNDSLGHPVGDRLLQAVSGRLAACARGSDTIARIGGDEFVVLLGDLKEPEDARHVA
                     QKVLDALSEPVMIDSHELKVTPSIGICAYPHDGEDVEALMRNADTAMYHAKQMGRNNY
                     QFFTQAMNDAAQQRLLLENDLRHALERGEFALHYQPQIDLKSGEIIGFEALVRWTHPQ
                     RGQVAPSRFIPAAEEAGLIGPIGEWVLRQACSQARIWHESGHRRLQVAVNCSAQQFQQ
                     AGFDETVRQILRESSVPAERLELEITESIIIQRSEEVIARFRALDNMGVRISIDDFGT
                     GYSGLSYLKRLAIHQLKIDQSFVRDISSDPDDAAIVSAIIAIAHSLGLEVVAEGVETA
                     EQLAFLKSLGCDGAQGYYFSKPLPADEFTRLLAGWSQQKRVAVEA"
     gene            4225..>4577
                     /locus_tag="AC158Q_13425"
     CDS             4225..>4577
                     /locus_tag="AC158Q_13425"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011544784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine(46)-N(7))-methyltransferase TrmB"
                     /protein_id="HIR3340815.1"
                     /translation="MKPLHRPVRSYVLRQGRITDAQRRSRDELMPRLGIPYSRERLDL
                     DALFGRVAPKILEIGFGMGETTARIAADHPGNDYVGIEVHTPGVGSLLKRIAELGVGN
                     LRLIQHDAVDVVEHM"
BASE COUNT          740 a         1565 c         1477 g          795 t
ORIGIN      
        1 aagcgagcgg ggccgaaatc gtcaccgtcg cgatccggcg caccaacatc ggccaggacg
       61 ccgggcagcc gagcctgctc gactacctgc cgccgtcgaa gttcgccctg ctccccaaca
      121 ccgccggctg ctatacggcc gacgacgccg tgcgcaccct gcgtcttgcg cgggacttgc
      181 tggacggcca cgacctggtg aaactggagg tgctgggcga ccccaggacg ctctacccca
      241 acgtggtcga caccctcgcc gcggcgaaga cgctggtcag ggacggcttc aaggtgatgg
      301 tctacacctc ggacgatccc atcatcgcga aacagctcga ggaaatcggc tgcgtggcgg
      361 taatgccgct cgcctccctg atcggctcgg gcatgggcat cctgaaccca tggaacctgc
      421 agatcattat cgagagcgcc cgggtgccgg tgatcgtgga tgccggcgtc ggaaccgctt
      481 ccgacgcggc gatcgcgatg gagctcggct gcgacggcgt gctcatgaac accgcggtcg
      541 ctgccgcggg caacccggtt ctgatggccg gcgcgatgaa gaaagcggtc gaagcgggcc
      601 gcgcggcgtt tcttgcgggc cgcatgccga agaagctcta ctcgggctcg cccagctcgc
      661 ccacgacggg gcttgttact ccgcccgccc cgtccaaggg atgagggcgg cgggcggggg
      721 aatgaggaag cgaacttgag gttcgccggg ttttccctca tccctcatca atcatccctc
      781 ctccctgctt taagcttcca cggccacgcg tttctgttga ctccagccgg ccagtaaccg
      841 ggtaaattcg tctgccggca gcggcttgct gaaataatag ccctgcgccc cgtcacaacc
      901 cagcgacttc aggaacgcca gttgctcggc tgtttccacg ccctccgcca ccacttcgag
      961 accgagactg tgcgcgatgg cgatgatcgc gctgacgatg gccgcgtcat cgggatccga
     1021 gctgatgtcg cgcacgaacg actggtcgat cttcagctgg tggatcgcga ggcgcttcag
     1081 gtaggagagg cccgagtaac cggtgccgaa gtcgtcgatc gagatgcgca cgcccatgtt
     1141 gtccagcgcc cggaaacggg caatcacttc ctccgagcgc tggatgatga tgctctccgt
     1201 gatctccagc tcgaggcgtt cggcgggcac actgctttcc cgcaggattt gccgcacggt
     1261 ttcgtcgaaa cccgcttgct ggaactgctg cgccgagcaa ttgaccgcca cctgcaagcg
     1321 ccggtggccc gattcgtgcc agatccgggc ctgcgagcag gcctgccgca gcacccactc
     1381 cccgatcggc ccgatcaggc cggcttcctc ggccgccggg atgaatcggg acggcgcgac
     1441 ctggccgcgc tgcgggtgcg tccagcgcac taacgcctcg aaaccgatga tttcccccga
     1501 cttcaggtcg atctgcggct ggtaatgcag cgcgaactcg ccgcgctcca gggcgtggcg
     1561 caggtcgttc tccagcagca ggcgctgctg cgccgcgtcg ttcatcgcct gggtgaagaa
     1621 ctggtagttg ttgcgaccca tctgcttcgc gtgatacatc gcggtatccg cgttgcgcat
     1681 gagcgcctcg acgtcctcgc cgtcgtgggg gtaggcgcag atgccgatgc tgggcgtcac
     1741 tttcagctcg tgcgagtcga tcatcaccgg ctcggagagc gcgtcgagca ccttctgcgc
     1801 cacgtggcgg gcatcctcgg gctccttgag gtccccgagc agcaccacga actcgtcgcc
     1861 gccgatgcgg gcgatcgtgt cggagccgcg cgcgcacgcg gccaggcgcc ccgacacggc
     1921 ctgcagcagg cggtcgccga ccgggtgccc gagcgagtcg ttgaccgtct tgaagcggtc
     1981 gagatcgatg aacagcaggg cgatcgattc gcggttgcgc tgcgcctgca cgatcgcctg
     2041 gccgatgcgg tcgtagagca gcgtccggtt gggcagctgg gtgagcgcat cgtggtgggc
     2101 catgtgccgg attttttcct cggtctgctt ctgttcggtg acgtcccggc ccgtgccgcg
     2161 atagccccgg aaacggccct gctcgtcgaa gatcggccgc ccgctcacgc tgatcacgcg
     2221 caggttgccg tcggcatcgt ggcgctggta cgtgaaatcc tggaacggct cgcgcgccgc
     2281 gagcaggccc ttgtgcatct cccactgatc cgcgctgacg ccgagtatcg gcatttccca
     2341 gcgcgtcctg ccgatgtgct cttcggcgtc caggctgatc tcgtggatct tcgacgacaa
     2401 ctgggtgaaa cggtaattcg cgtcctgctc ccagtaccag tccgaggaca gttccgtcag
     2461 gtcgcgaaag cgctcttcgc tctggcgcaa tgccgcttcc gcttccttca agccggtcac
     2521 gtcggtgatg gttccgatga ggccggccgc ggaaccgtcg aacttgttga aagtcgcctt
     2581 gttgtagatg gtgtgatgca cccggccgtc ggccgcgatg atggccgcct cgtaggactg
     2641 gctgccgggg cgtccgaaca gctcctggtc cttggcgtgg tgttttctcg ccagttcgag
     2701 gttgttcgga tacagctcga acaccgattt gccgatgaac tgctcgcgcg gtatgccgaa
     2761 aaacccctcc cacgccttgt tgacgccgag gtagcggccc tcgacgtcct tgaagaagat
     2821 cggctgcggc acagcttcga tcagctgctg gatgaaggcg agctgctcgc gcaatccctc
     2881 cgcggcgctc atcgcccggg cgtgctcggc gaacctcagc gcgcgctcgc gcgagaccag
     2941 gagcgtccac acgccccaga acagcagcag gctggtgagg ataccgccca tcaacgtcaa
     3001 cgggggcagc gcactgctga tgccgtgtgc cggtatcctg ggcgccctga actccagccg
     3061 ccagtggcgc ccggccacgt cgagggtcat cacctggctc aagaaggcgt gccccgcctc
     3121 ttttaccgcc ttcatgccga atgtccggct gctgtcgaat accaggtcgt cccggccccg
     3181 tccggcgggc tgcccgtcca ttcccgaaaa tcccaggtcg tggatggcga ggtcgaattc
     3241 gctgccgagc tcccggccca gctggctgcc gacgaggtcg tccacgcgga tcgcggacga
     3301 caccacgccg aggaacgcgg cgcggcgctc ctcgagggtg ccggccggcg cgccccggcg
     3361 gtacaggggc atgcgcagcg ccagcgagat ctgcttctgt ggatccgcgg cgagcaagat
     3421 cggctcggaa gccgtcacct gcccgctatc gcgcgcgcgc tccacggagg cgcgccgctg
     3481 cggttcggaa tacacatcga acccgaacgc cccttcgttg ccggcgaacg gctcgatgta
     3541 ggtcaccacc atatagtcgg cgcggtcgcc ggcgggcttg atcgcgaaat ccgggtagcc
     3601 ccgggagtcg acgctgctgt cgcgccgcac gctctcctcg aacccggcct tctccgccgg
     3661 agtcacgtaa cgcacgaacg acatcagccg gatgccggaa tagcgctgcg tgacgttgaa
     3721 tccggagaga tagagccgga attcgtcccg gtcgacccga ttggaagcgg caaagaaacc
     3781 ccggagaccg atcagcaggt tgacgttgtc ctgcatgcgg cggtcgagcg tgcccatggc
     3841 ctgcgcgaca tggttctgaa acttcgcctc ggcttcccgg ccgacccgct gctcagtggt
     3901 gttccaggca gcgaaagaaa tcgcgaggcc cacggccagc acgagaaacg ccggccaggt
     3961 gagcccggcg cgcccggcga tcgccgccaa cgggctgccc ctgctaccgg ccagcatgat
     4021 gtctctcccc cgggggtatt tgtcttacct tacccccttt gcgaggcgcc ttcaaaccct
     4081 gtacccgggg caggttgctt cggcaggaag gcggcggcgc gcggtcgggc cggacctgcc
     4141 tgtgccgtgt cgaatctgag agaatcgcct gcagcgcggc ccccggccag ccggatacgg
     4201 cagcggcccg ccgtgctccc gcccatgaaa ccactccacc gcccggtccg cagctatgtc
     4261 cttcgccaag gacgcatcac cgacgcgcaa cggcggtccc gcgacgagtt gatgccgcgg
     4321 ttgggcattc cctactcgcg cgaacggctc gacctcgacg ccttgttcgg gcgcgtggcg
     4381 ccgaagatcc tcgagatcgg cttcggcatg ggagaaacaa cggcccggat cgccgccgat
     4441 catcccggca acgactacgt cggcatcgag gtccacacgc ccggcgtggg cagcctgctc
     4501 aagcgcatcg ccgaactcgg cgtcggcaac ctgcgcctca tccagcacga tgcggtggac
     4561 gtcgtcgagc acatgat
//
LOCUS       DBNGSG010000504         9121 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_335365, whole genome shotgun sequence.
ACCESSION   DBNGSG010000504 DBNGSG010000000
VERSION     DBNGSG010000504.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9121)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9121)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9121
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_335365"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1736)
                     /locus_tag="AC158Q_13430"
     CDS             complement(<1..1736)
                     /locus_tag="AC158Q_13430"
                     /inference="COORDINATES: protein motif:HMM:NF012550.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HIR3340816.1"
                     /translation="MAAVDSTPEMAQKVYEAMARNLGAVRRRLKRPLTLSEKVLLGHL
                     DDPERQALAPGKSYLALRPDRVVFQDVLGQTGMLQFMQTRRPRVAVPTTIHCDHLIQA
                     RNDGARDLRESLAENTEVYDFLRSAAARYGAGFWEPGAGIIHQVVLESYAFPGELIIG
                     TDSHTPNAGGLGACAVGVGGADAVETIAGLPWEVLYPKRIAVYLTGRLGGWTAPKDVI
                     LYVAGVLTVSGGTNAIVEYIGPGARTISATGKATVANMGAELGATTSMFPADERMALY
                     LRATGRGGLAEQMGKYRSLLEPDPEVEADPEAHYDRVVRLDLSTLEPHVVGPHSPDRA
                     RPISRLAAEVADPKNGFVDRVSAALVGSCTNSSYEDMSRAADVARQATARGLKAAAPY
                     LVTPGSEQVRATIERDGQMRSLADIGAAVLANACGPCIGQWRRAKETSAAPNTIVTSY
                     NRNFPARNDGQPTTMNFIASPEIVTALAVAGRLTFNPLTDTLVGADGKPFKLEAPKIA
                     PEVPARNFERGRDLYVAPPADGSAITVSVSPQSERLQIMEPWPAWDGKDLTDMPVLLK
                     AKGKTTTDHISPA"
     gene            1829..2695
                     /locus_tag="AC158Q_13435"
     CDS             1829..2695
                     /locus_tag="AC158Q_13435"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR3340817.1"
                     /translation="MLDGIEALIALEKFGTISEAAVRLRLTQSAVSKRIQALQRAVGL
                     PLIERDGRRVRLTAPAVDFLERARPLVADLRGLTGPQHGAAPTFSIALADSIASSWGP
                     AVIQRALQGLDGISVRLHAHRSVLLVESVRLGRYHIGLSTDLPAAKDLIHYPVVDEPL
                     VLVRSGLGVKTDRGQPLISIEPSSATWRAIEPQLRELHPQLLARPLVPVETFSATVQM
                     VKAGFGDGLVPLGLAIEMELDRGSYREVRGVRRQISLVTRKTVNQLAGFKLLRERLVS
                     EAARYFATRKSA"
     gene            complement(2687..3565)
                     /locus_tag="AC158Q_13440"
     CDS             complement(2687..3565)
                     /locus_tag="AC158Q_13440"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF024994.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009361 - succinate-CoA ligase complex
                     (ADP-forming) [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004775 - succinate-CoA ligase
                     (ADP-forming) activity [Evidence IEA];
                     GO_function: GO:0004776 - succinate-CoA ligase
                     (GDP-forming) activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HIR3340818.1"
                     /translation="MTINPRIDRNTPVIVQGITGRAGQMHSRLMMNYGTRIVGGVSPG
                     AKVSEVNGVPVFTNCAEAVVKTGASASVLLVGAMQLLEAMRDAVAAGIRYLVTPTEGM
                     PVHDALKAWKLCRDTETVWIGGSTPGMAVPGEAKLGFLPDDSLKPGPLGLMSKSGTLS
                     YEAGYRLALRGIGTSVWVGVGGDPVKGARYADLVPFYEADEQTKALLIIGEIGGAEEE
                     EFAQALVAHEFNKPAFALIAGRSAPEGVTMGHAGALVHGSHGTYAAKRAALEAAGVQV
                     FGALNEMVAGISARLR"
     gene            complement(3676..4803)
                     /locus_tag="AC158Q_13445"
     CDS             complement(3676..4803)
                     /locus_tag="AC158Q_13445"
                     /inference="COORDINATES: protein motif:HMM:NF020031.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR3340819.1"
                     /translation="MYLLEHDAKELLAAHGIPVPAGCLIEHLTAQPALPAGPWIVKGQ
                     ITAGGRGKAGIIRKAATPREVAEHVLAILGRSVKDRTVESVRVEQQVQAAQEIYISFL
                     LDASSGGVRVMLSANGGMDIEQVPHGQLLTGLARPEEKALIECVDQLAGKLPSGVATA
                     AREAGHRLARAFLGLECMLVEINPLFVLDGGRWVAGDAKVVTDDNALPRQKALESLVK
                     ARAAAYPDVARKHEHGFDYVEVDPEGEIGLLTTGAGLSMMLIDELRAAGLKPFNFLDI
                     RTGGLRGETTRLTQVLQWIGQGKKVKVLLVNIFAGITDLGEFSRLLVQALGAANLRPP
                     VVARLVGTNLAAARDTLAAAKIGLHTDLDAAIAEVRRHLNK"
     gene            complement(4884..5657)
                     /locus_tag="AC158Q_13450"
     CDS             complement(4884..5657)
                     /locus_tag="AC158Q_13450"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR3340820.1"
                     /translation="MAVRIEVRGQGHVAYVTVNNPERRNVLGIVGKKALAEAFRQLAK
                     DKALRVAVLTGAGERSFIAGADLAEMKDLKPNGAREEHTWTHRACESIRALPVPVIAR
                     INGYCFGAGMELAASCDLRIGVTTAKFGMPEVRFGIPSGMEACLLPQLIGWGKTRELV
                     YTGDHIDAAEAHRCGFLEKRVETAGLDAGVEQWISSIIAAGPRAIRLQKKLVRDWEHM
                     TISQAVQAGIKACVEARRTDEPRRLMQAFLERKKKGDKG"
     gene            complement(5708..7114)
                     /locus_tag="AC158Q_13455"
     CDS             complement(5708..7114)
                     /locus_tag="AC158Q_13455"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3340821.1"
                     /translation="MSTSRAPLLANATRELASFASRLKYEDIPAAAVERMKHCVLDTI
                     GCCLHGVTLPWTRKVQEMVLAEGGAPAASIFGGGGRTSVSQAVLVNGTAGHAFELDDI
                     HKESIVHPGSLAMPVAVALAEAAGGVSGRDLITAMVAGYEVGTRVGNAATMSLFLRGF
                     HPQGTSGVFVAAATAARMLGLDPAQMQHTLGIAGSQAGGLMAAQEGAMVKRFHCGRAA
                     QSGVYSALLARRGFTGITDVLEAPYGGFLSSLSDQPAPARLTAGLGTVWETVNVGYKP
                     HASVTSIHTALDALADLMRENRLAANDIKHVDAGLSHMTHVHCAWQYEAQGVTAAQMN
                     LYYGLAVIALDGAAFVEQYREDRLRDPRILDFIGRIEARVDPEIEAMGPAFRHAARVR
                     VETRDGRAFTREILHRRGSPENPLKPGDIEYKFRNVARACLAPPKLERVIELCGSLDR
                     LPDTRELTAILAAPAGAP"
     gene            complement(7211..8107)
                     /locus_tag="AC158Q_13460"
     CDS             complement(7211..8107)
                     /locus_tag="AC158Q_13460"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA dioxygenase family protein"
                     /protein_id="HIR3340822.1"
                     /translation="MIIINKLGRHLGAEISGVDLSQPLEDSTFAKISKAFFDHEVVVF
                     RNQKITPEQQIAFTRRFGVLEQHVRKEHRLDGYPEILIVSNVLNSEGKAIGVEDAGRF
                     WHSDLSYKQVPSLLSALYAIEVPVKDGVVLGHTNFASTTAAYEALPEDMQRKLGRLKN
                     VHSYVYYRGKNIQAQKEEQARGDRVVQEHVLTPEHLAQVPDAEMPIVRTHPVTGKKGL
                     FINEAHTSHIVGLPKPEGAALLKEICAHIIKPEFQYEHHWKAGDLLMWDNVAVQHKAN
                     FNYRLPLRRLMYRTTVRGTAPF"
     gene            8265..>9121
                     /locus_tag="AC158Q_13465"
     CDS             8265..>9121
                     /locus_tag="AC158Q_13465"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HIR3340823.1"
                     /translation="MTLQQLRILREIARQAHNLSSAAAALHTSQPGVSRQIQLLEREL
                     GVELLVRSKNRVTGMTEPGRAVLAAAQRALGEADNIRLIAAEYGREEGGRLTIATSHL
                     HARYTLLTPIKTFALRHPGVRLHMRQADPDEILRLVASGEADIGVSTEVSAERADLLL
                     LPGRVVERSLIMPLGHMLARKEKVTLADIAKYPLVGYDPKQRGGQIISGTFRAKGHEL
                     KLVVSAIDSDIIKAYVAEGVGIAVIPTLALDAELDRGLRVVDVTRLFPKSVMTISLRR
                     DIYLRRYVP"
BASE COUNT         1393 a         3000 c         3140 g         1588 t
ORIGIN      
        1 gccggcgaga tgtggtcggt ggtggtcttg cccttcgcct tcagcagcac gggcatgtcg
       61 gtcaggtcct tgccgtccca cgccggccac ggctccatga tctgcagccg ctcgctctgc
      121 gggctgaccg acaccgtgat cgcgctgccg tcggccggcg gcgccacgta gaggtcgcgg
      181 ccccgttcga agtttcgcgc cggcacctcg ggcgcgatct tcggcgcctc gagcttgaac
      241 ggcttgccgt ccgcgccgac gagcgtatcg gtgagcgggt tgaacgtgag ccgcccggcc
      301 accgcgagcg cggtgacgat ctcgggactc gcgatgaagt tcatcgtggt cggctggccg
      361 tcgttgcgtg ccgggaaatt gcggttgtac gaggtgacga tggtgttggg cgcggcggac
      421 gtttccttcg ccctgcgcca ctgaccgata caggggccgc aggcgttcgc cagcaccgcc
      481 gcgccgatgt cggcgagcga gcgcatctgg ccgtcgcgct cgatcgtcgc gcgcacctgc
      541 tccgagccgg gggtgacgag gtagggcgcg gcggccttga ggccgcgcgc cgtcgcctgc
      601 cgcgccacgt ccgcggcgcg gctcatgtcc tcgtaggacg agttggtgca gctgccgacg
      661 agcgcggccg agacgcggtc gacgaatccg tttttcgggt cggccacttc cgccgccagc
      721 cgcgagatcg ggcgcgcgcg gtcgggggag tgcgggccga cgacgtgggg ctccagcgtc
      781 gagaggtcga gccgcaccac gcggtcgtag tgcgcctcgg ggtcggcctc gacctccggg
      841 tccggttcca gcaggctgcg gtatttcccc atctgctccg cgaggccgcc gcggccggtc
      901 gcgcgcaggt acagcgccat gcgctcgtcg gcggggaaca tcgaggtcgt tgcgcccagc
      961 tcggcgccca tgttggcgac ggtcgccttt ccggtcgcgc tgatggtgcg cgcgccgggc
     1021 ccgatgtatt cgacgatggc gttggtgccg ccggataccg tcagcacgcc tgcgacgtag
     1081 aggatcacgt ccttcggcgc agtccagccg cccagcctgc cggtcaggta gaccgcgatg
     1141 cgcttcgggt acagcacttc ccacggcagc ccggcaatgg tctcgacggc atccgcgccg
     1201 ccaacgccga ccgcgcacgc gccgaggcct ccggcgttcg gagtgtgcga atcggtgccg
     1261 atgatgagct cgcccgggaa cgcatagctc tccagcacca cctggtggat gatgcccgcg
     1321 cccggttccc agaagcccgc gccgtaccgg gccgccgcgg agcgcaagaa atcgtagacc
     1381 tcggtgttct cggccagcga ctcgcgcagg tcgcgcgcgc cgtcgttgcg cgcctggatc
     1441 aggtggtcgc agtgaatcgt cgtcggcacc gccacgcgcg ggcgccgcgt ctgcatgaac
     1501 tgcagcatgc cggtctggcc gagcacgtcc tggaacacca cgcggtcggg gcgcagcgcg
     1561 aggtagctct tgcccggcgc gagcgcctgg cgctccgggt cgtcgaggtg ccccagcagc
     1621 accttctcgg agagcgtcag ggggcgcttc aggcggcggc ggacggcgcc cagattacgg
     1681 gccatggcct cgtagacctt ttgcgccatt tcgggggtgg aatcgacagc ggccatacgc
     1741 gggtctcctc gtgcgggcgg ttggatgggc cggagtctag ctataaaatg cgagaataag
     1801 tttcatacaa ttcagtctta atgggaatat gttggacgga atcgaggcgc tgatcgccct
     1861 ggagaagttc ggcaccatca gcgaggccgc ggtccggctg cgcctgacgc agtcggccgt
     1921 cagcaagcgc atccaggcgc tgcagcgcgc cgtcgggctg ccgctgatcg agcgcgacgg
     1981 ccgccgcgtg cggctgaccg cgcctgccgt cgactttctc gagcgtgccc ggccgctggt
     2041 cgccgacctg cgcggcctga ccggcccgca gcacggcgcc gctccgacgt tctccattgc
     2101 gctcgccgat tcgatcgcgt cctcgtgggg gcccgcggtg atccagcgcg cgctgcaggg
     2161 gctcgacggc atcagcgtgc gattgcacgc gcaccgcagc gtgttgctgg tggaaagcgt
     2221 acggctgggc cgctaccaca tcggcctcag cactgatctt ccggctgcga aggatctgat
     2281 tcattacccg gtggtggacg agccgctggt actggtgcgt tcggggctgg gcgtgaaaac
     2341 cgaccggggc cagccgctga tctcgatcga gccctcctcg gcgacctggc gcgccatcga
     2401 gccgcagctg agggagctcc acccgcagct gctggcacgg ccgctggtgc cggttgagac
     2461 cttcagcgcg acggtgcaga tggtcaaggc cggtttcggc gatggcctgg tgccgctggg
     2521 gctggcgatc gagatggagc tcgaccgggg cagctaccgc gaagtccgcg gcgtgcgcag
     2581 gcagatctcg ctggtgacga gaaagacggt caaccagctg gccggcttca agctgctgcg
     2641 ggagcgtctg gtgagcgaag cggcgcgcta cttcgccacc aggaagtcag cgtaggcggg
     2701 cgctgatccc ggcgaccatc tcgttgaggg cgccgaacac ctggacgccg gcagcttcga
     2761 gcgcggcgcg cttggcggca taggtgccat ggctgccgtg gaccagcgcg ccggcatgtc
     2821 ccatggtgac gccttccggc gcgctgcggc cggcgatcag cgcgaacgcc ggtttattga
     2881 actcgtgcgc gaccagcgct tgcgcgaact cttcttcctc ggctccgccg atctcgccga
     2941 tgatgagcag cgcctttgtc tgctcgtcgg cctcgtagaa gggcacgagg tcggcatagc
     3001 gcgcgccctt gaccgggtcg ccgccgacgc cgacccacac cgaggtgccg ataccgcgca
     3061 gggcgagccg gtagccggct tcgtaggaga gggttcccga cttggacatg aggccgagcg
     3121 ggccgggctt cagcgagtcg tccggcagga agccgagttt cgcttcgccc ggcaccgcca
     3181 tgcccggcgt ggagccgccg atccagacgg tttccgtgtc gcggcacagt ttccaggcct
     3241 tcaacgcgtc gtgcaccggc atgccttcgg tgggggtgac gaggtaacgg ataccggctg
     3301 cgacggcgtc gcgcatcgct tcgagcagct gcatcgcgcc caccagcagc acgctcgcgc
     3361 ttgcgccggt tttcacgacc gcctcggcgc agttcgtgaa taccgggacg ccgtttacct
     3421 cgctcacctt cgcgccgggc gagacgccgc cgacgatgcg ggttccgtaa ttcatcatca
     3481 ggcggctgtg catctgcccg gcgcggccgg ttataccctg gacgatgaca ggggtatttc
     3541 tatctattct ggggttgatg gtcataagga gtgactcgtt tcccgttcct cgttcctggt
     3601 gtcaacgaaa agcgcttcgc gaaatattgc gttatccaaa aagcgggaaa cgagaaacga
     3661 acaacgagaa acgagtcact tattcaaatg ccgtcgcacc tcggcgatcg ccgcgtcgag
     3721 atcggtgtgc aggccgatct tcgccgcggc gagagtatcg cgggcggcgg caaggttggt
     3781 gccgaccagc cgcgcgacca ccggcggccg gagattcgcg gcgcccagcg cctggaccag
     3841 tagccgcgag aactcgccga gatcggtgat gccggcgaag atgttgacca gcagcacctt
     3901 cacctttttt ccctggccga tccactgcag cacctgtgtc aggcgcgtgg tctcgcctcg
     3961 caatcccccg gtgcggatgt cgaggaaatt gaagggcttc agtcccgctg cgcgcagttc
     4021 gtcgatcagc atcatcgaca gcccggcgcc ggtggtgagg agcccgatct cgccttcggg
     4081 atccacctcg acatagtcga aaccgtgctc gtgcttgcgc gcgacgtcgg ggtaggccgc
     4141 cgcgcgggcc ttcaccaggg attccagcgc cttctgccgg ggcaaggcat tgtcgtcggt
     4201 gacgaccttg gcgtcgcccg cgacccagcg gccgccgtcc agcacgaaca ggggattgat
     4261 ctcgaccagc atgcactcga gcccgaggaa agcgcgggcg agccggtgac ccgcctcgcg
     4321 ggctgccgtc gccacgccgg acggcagctt gccggccagt tggtccacgc actcgatgag
     4381 cgccttttcc tccgggcggg cgaggccggt cagaagctgc ccgtgcggca cctgctcgat
     4441 gtccatgccg ccgttcgcgg agagcatcac gcgcacgccg ccgctgctgg cgtcgagcag
     4501 gaagctgata tagatctcct gcgccgcctg gacctgctgc tccacgcgca cggactcgac
     4561 ggtgcggtcc ttgaccgacc tgccgaggat cgcgaggacg tgttcagcca cttcccgggg
     4621 cgtcgcagct ttcctgatga tgccggcctt gccgcggcca ccggccgtga tctggccctt
     4681 cacgatccac gggccggcag gcagggcggg ctgtgccgtg aggtgttcga tcaggcaacc
     4741 ggcggggacg gggatgccgt gtgcggcgag gagctccttg gcgtcgtgct ccagcaaata
     4801 catagagagg cgtgataagt gatgagggat aggggatagg gctcgaggtt ccgcgcgggt
     4861 gcgggtgcct tatcccttat cacctatccc ttgtcacctt tctttttgcg ttcgaggaat
     4921 gcctgcatga gccgcctggg ctcatccgtg cggcgggctt cgacgcaggc cttgatcccg
     4981 gcctggaccg cctggctgat cgtcatgtgc tcccagtcgc gaacgagttt cttctgcagg
     5041 cggatggcgc gcgggccggc ggcgattata gaggaaatcc attgctcgac gccggcgtcg
     5101 agtcccgcgg tctcgacccg tttttcaaga aaaccgcatc ggtgggcttc cgccgcgtca
     5161 atgtgatcgc cggtgtaaac cagctcccgg gtcttgcccc agccgatgag ctgcggcagc
     5221 aggcaggcct ccatgccgga ggggatgccg aatcgcactt cgggcatgcc gaatttcgcg
     5281 gtggtgaccc cgatgcgcag gtcgcaggaa gcggccagct ccatgccggc gccgaagcag
     5341 tagccgttga tgcgcgcgat gacgggcacg ggcagggcgc ggatggactc gcaggcgcgg
     5401 tgggtccagg tgtgctcttc tcttgccccg ttcggcttga gatccttcat ttccgcaaga
     5461 tcggccccgg cgatgaacga acgctctccc gcgccggtga gcaccgcgac gcgcagggcc
     5521 ttgtccttcg cgagttgcct gaatgcttcc gccagggctt ttttcccgac gatgccgagc
     5581 acgttgcgcc gctccgggtt gttgaccgtc acgtacgcga cgtgtccctg cccgcgcact
     5641 tcgatgcgca cggccatcag cggactccgg gtcgattcac gaatcacgat tcacgtctca
     5701 cgcgcactca cggcgcaccg gccggcgcag ccaggatggc ggtaagctcg cgggtgtccg
     5761 gcaggcgatc gagcgacccg cacaactcga tcacgcgctc gagcttgggc ggcgcgaggc
     5821 acgcgcgcgc cacgttccgg aacttgtatt cgatatcgcc gggcttgagg ggattttccg
     5881 ggctgccgcg gcgatgcagg atttcgcgcg tgaacgctcg gccatcgcgg gtctcgaccc
     5941 tgacccgcgc ggcatggcgg aaggcgggac ccatggcttc gatttccgga tccacgcggg
     6001 cctcgatgcg cccgatgaaa tcgaggatgc gcgggtcgcg caggcggtct tcgcggtact
     6061 gctcgacgaa agccgcgccg tcgagcgcga tcaccgcgag accgtagtac aggttcatct
     6121 gcgccgccgt gacgccctgt gcctcgtact gccacgcgca gtggacgtgg gtcatgtggc
     6181 tcaacccggc atccacgtgc ttgatgtcgt tcgcagcgag ccggttttcg cgcataaggt
     6241 cggcgagcgc atcgagcgcc gtatggatgc tggtgacgga ggcgtggggc ttgtagccga
     6301 cgttgacggt ttcccagacg gtccccagcc cggcggtgag cctggcgggc gcgggctggt
     6361 cggacagcga gctgaggaat ccgccgtagg gggcctcgag cacgtcggtg atgccggtga
     6421 agccgcggcg ggcgagcagg gcggaataga cgccgctctg ggcggcacgt ccgcaatgga
     6481 agcgcttcac catggcacct tcctgcgccg ccatcagccc gccggcctgc gagccggcga
     6541 tgccgagcgt gtgctgcatt tgcgccgggt cgagtccgag catcctggct gcggtggctg
     6601 cggcaacgaa cacgccggag gtgccctggg gatggaagcc gcgcaggaac aggctcatgg
     6661 tcgccgcgtt gcccacgcgc gtgccgactt catagcccgc caccatggcg gtgatcaggt
     6721 cgcggccact cacgccgccg gcggcctcgg cgagcgccac cgccaccggc atcgcgagcg
     6781 agccggggtg aacgatggat tccttgtgga tgtcgtccag ctcgaaggcg tggcccgccg
     6841 tgccgttcac cagcacggcc tgcgacaccg aggtcctgcc gccgccgccg aagatcgagg
     6901 cggcaggggc gccgccttcc gcgagcacca tttcctgcac cttgcgcgtc cacggcagcg
     6961 tcacgccgtg caggcagcag ccgatagtgt cgagcacgca gtgcttcatg cgctcgaccg
     7021 cggcagccgg gatgtcctcg tacttcaggc gcgaggcgaa cgacgcgagt tcgcgcgtcg
     7081 cgttcgcgag cagcggcgcc cgggaagtgc tcatgggccg tcattcccgc gcaagcggga
     7141 acccagtgga tggttcaatc gtcctgcctt cggttttctg gatcctcgct tccgcgagga
     7201 tgacgacatt ctagaacggc gcagttcccc tgaccgtggt ccggtacatc aaccgcctca
     7261 gcggcagccg gtaattgaaa ttcgccttgt gctgcacggc gacgttgtcc cacatgagca
     7321 ggtcgcccgc cttccagtga tgctcgtact ggaattccgg cttgatgatg tgcgcgcaga
     7381 tctccttgag cagggctgcg ccctcgggct tcggcagccc gacgatatgc gaggtatgcg
     7441 cctcgttgat gaacaatccc ttcttcccgg tgacggggtg ggtgcgcacg atcggcatct
     7501 cggcgtccgg cacctgggca agatgctccg gggtcagcac gtgctcctga acgacacggt
     7561 cgccgcgcgc ctgctcttcc ttctgcgcct ggatgttctt gccgcggtaa tagacgtagc
     7621 tgtgcacgtt cttcagtcgg ccgagcttgc gctgcatgtc ctcgggcagc gcctcgtagg
     7681 cggccgtcgt gctggcgaaa ttggtatggc ccagcacgac gccgtccttc accggcactt
     7741 cgatggcgta gagcgcggac agcaggctcg gcacctgctt gtaggagagg tcgctgtgcc
     7801 agaagcggcc ggcatcctcg accccgatcg ccttgccctc gctgttgagg acattggaaa
     7861 cgatcaggat ttccgggtag ccgtcgagcc ggtgctcctt gcgcacgtgc tgctcgagca
     7921 cgccgaagcg gcgggtgaag gcgatctgct gctcgggcgt gatcttctgg ttgcggaaga
     7981 ccacgacctc gtggtcgaag aacgccttgg agattttcgc gaacgtgctg tcttccaggg
     8041 gctgcgaaag gtcgacgccg ctgatttccg cgccgagatg ccggcccagc ttgttgatga
     8101 taatcatatg atttcttgag gataaatcat tcatttccga ctcgcttgtg ggttctccgg
     8161 gctggccggg accgccgaat actagcggcg cccagccatg ccgtaaaaga attaatatga
     8221 atgaaatcat gttatttgga tatgaccctg gttatcatgc cgccatgacg ctccagcaac
     8281 tgcgcatcct gcgcgagatc gcgcgccagg cgcacaacct ctccagcgcg gccgctgcgc
     8341 tgcatacctc gcaacccggc gtcagccgcc agatccagct gctcgagcgc gaactggggg
     8401 tggagctgct cgtgcggagc aagaaccggg ttaccggcat gaccgagccg ggccgcgcgg
     8461 tccttgccgc cgcgcagcgg gcgctgggcg aagccgacaa catccggctg atcgccgccg
     8521 agtacggccg cgaggagggc gggcgcctca cgatcgcgac cagccacctg cacgcgcgtt
     8581 acacgctgct gacgccgatc aagaccttcg ccttgcggca ccccggcgtg cgcctgcaca
     8641 tgcggcaggc cgatcccgac gagatcctgc ggctggtcgc cagcggcgag gccgacatcg
     8701 gcgtcagcac cgaggtgagc gcggagcgcg cggacctgct gctgctgccg gggcgggtgg
     8761 tcgagcgcag cctgatcatg ccgctgggac acatgctcgc caggaaagag aaggtgacgc
     8821 tcgccgatat cgcgaagtat ccgctggtcg gctacgaccc gaaacagcgc ggcgggcaga
     8881 tcatcagcgg gacgtttcgc gcgaaggggc acgagctcaa gctcgtcgtg agcgcgatcg
     8941 actcggacat catcaaggcc tacgtcgccg aaggggtcgg tatcgcggtg atacccacgc
     9001 tggcgctcga cgccgaactg gaccgcgggc tgcgcgtggt ggacgtcacg cgcctgttcc
     9061 cgaagagcgt aatgacaata agcctgcgcc gcgacatcta cctgcggcgc tacgtgcccg
     9121 a
//
LOCUS       DBNGSG010000505         7910 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1693505, whole genome shotgun sequence.
ACCESSION   DBNGSG010000505 DBNGSG010000000
VERSION     DBNGSG010000505.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7910)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7910)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7910
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1693505"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1369)
                     /gene="hemN"
                     /locus_tag="AC158Q_13470"
     CDS             complement(<1..1369)
                     /gene="hemN"
                     /locus_tag="AC158Q_13470"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HIR3340824.1"
                     /translation="MNSPTIEFDADLVRRLDRTGPRYTSYPTADRFIEAFDETRYTMW
                     AARRNVGGVQRPLALYVHLPFCRDICFYCGCNKIVTRDGSKAARYLDYLGREIDLQAA
                     LFRDDPRVAQMHWGGGTPTYHDMGDLRALFGRLTEQFDFAPRGEYSIEVDPRTVDAPA
                     MESLRDMGFNRVSFGVQDFDPAVQAAVHRLQPRERTLAVIDAARRAGFDSINVDLIYG
                     LPKQSPASFDTTLAQVVAARPGRVALYNYAHLPQLFKSQRRIREDDLPSSEGKLQLLE
                     LAIRLLTAAGYVYIGMDHFALPDDALAVAQRQGRLHRNFQGYSVLANADLVGLGVSAI
                     GAIGPTYSQNQRALEGYYDALDRGELPIMRGIELTADDLVRRAVIQALMCQFALSKES
                     IEVAYLLDFDRYFATELEELREFQRMGLVALEEGWLTVSAKGRLLIRSICMVFDRYLR
                     RDRDLR"
     gene            1470..2210
                     /gene="fnr"
                     /locus_tag="AC158Q_13475"
     CDS             1470..2210
                     /gene="fnr"
                     /locus_tag="AC158Q_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HIR3340825.1"
                     /translation="MKTPSRPPALDIGRLRVACSACSLRELCLPAGLEAPEMAKIDRL
                     VSQRRRVRKGEHLYHAGASLAALFAIRAGFFKSCVLHEDGREQVAGFHMMGDLLGMDA
                     IGGSRHASDTVALEDSEVCEMPFPLLEQLSREVPSLQQQLHRVMSREIVREHGVMLLL
                     GSMKAEERLAAFLLNLSQRFAARGYSPVQFNLRMTRQEIGSYLGLKLETVSRAFSAFQ
                     EQGLMTVRQRQVHIVDLSGLKAVLGAAR"
     gene            complement(2227..4659)
                     /locus_tag="AC158Q_13480"
     CDS             complement(2227..4659)
                     /locus_tag="AC158Q_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIR3340826.1"
                     /translation="MAYPGLSSAEARRRLDAEGPNRLPDAGRRGALAIALEVVREPMF
                     LLLVAGALVYLLLGDVREALVLAASIAVVMTITVAQERKSERALEALRDLSSPRALVI
                     RDGAPLRIAGTEVARGDLVLLAEGDRVPADARLDSVNDLMVDESLLTGESLPVEKRPD
                     LPVYSGTLVVRGQGRAEVTGTGPRTEFGKIGQSLAALDIEKTRLQTETARIVKAVAVF
                     AIALSLLLAAYHVATRGDWLAGILAGITLAIAILPEEFPVVLTVFLALGAWRIGRDGV
                     LTRRMPAIETLGAATVLCVDKTGTLTENRMALVDAISTDAAPEYVIEAAALACELEPF
                     DPMERAILAAAGGHAAELRRSWTLERDYPLTPDFLAVCHAWRSPSGGRRVAVKGAAET
                     VMELCALDETARAAADAEMRRAAESGLRLLAVAEAGWADAEWPADPRQYAFRWLGFVA
                     LADPLRARVPEAVALCRGAGIRVVMITGDYPGTALAIARQAGIDAGAGALTGTEISAM
                     DAPALAEAARRVNVFARIRPEQKLQLITAYKTDGEIVAMTGDGVNDAPALKAAHIGVA
                     MGRRGTDVAREASALVLLEDDFQSIVGAVRLGRRIYENIRNAMRYLLAVHVPIAGMSF
                     LPLVFGWPVFIYPVHVVFLEFVIDPACSIVFEAEKSEEGAMTRPPRDPREPLFSLQML
                     GVSLALGASVLLAVCLAYGWVVGNGLTGGEAGSFGFAAIVFGNLAMIHATRSRDRLIL
                     DALRSPNPALWWITTGTLVALAASIYVTPIAELFRFAPLPPGYLALAAAAGVAGVLWY
                     EAYKWLRPRGRA"
     gene            complement(4684..5526)
                     /locus_tag="AC158Q_13485"
     CDS             complement(4684..5526)
                     /locus_tag="AC158Q_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HIR3340827.1"
                     /translation="MEMFGFDSFSPREIAGRVKDFCVIKARLPLLTLWMLGMLAGAFI
                     GLGSLYFTLVVSDASIGFAAARVAGGVCFSLGLLLVVVAGAELFTGNNLLAMGWADGC
                     VSLGEVLRNWVVVCVANFAGAAGLAVIVFFSGHWEMNGGAVGAAYLKIAATKAALPVG
                     RAFLLGVLCNILVCMAVWMALAGRSVVDKFIAIVFPISAFVAAGFEHSIANMYFFPLA
                     MLLKWHGLALPPGGDAITVAGMLGNLGPVIAGNIIGGSVLVALVYWVIYLRGRPPDGA
                     APRA"
     gene            complement(5745..6704)
                     /locus_tag="AC158Q_13490"
     CDS             complement(5745..6704)
                     /locus_tag="AC158Q_13490"
                     /inference="COORDINATES: protein motif:HMM:NF013895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIR3340828.1"
                     /translation="MRRETVGAHLVASVGRGHRDETVAAMVARLAAEKVASVDPVCVT
                     DGSGLLLGAIPAGRLFTANGGALLAEIMDPAFPRVHAEDDQEVAASVALHHGVDGLPV
                     VDHDGRLLGVMPAQALLQALRREHVEDLHVLAGIRRESVQARHAIEDPPMRSARHRLP
                     WLLVGLGGGAVATAAMATFEATLEKTVAVAFFVPALVYIADAIGTQTEAIAVRGLSLT
                     RTGIGHLLSVELRTGMLIGLTLGALAFLPIWFAFGNARLAAAVSLSIVIAGTIAAAIG
                     LALPWLFARRGLDPAYGSGPVATVIQDILSILVYFAVLRAFGI"
     gene            complement(6704..>7910)
                     /locus_tag="AC158Q_13495"
     CDS             complement(6704..>7910)
                     /locus_tag="AC158Q_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HIR3340829.1"
                     /translation="GKRSEAYDRNKNLPFNANSIDACILSHAHIDHSGLLPRLVAAGF
                     EGAVYATRPTCDLLGVMLPDSGHLQEREAEREGGAPRYTDPQARASLRRLQPVEYGVE
                     VRPHPSVRCVFRDAGHILGSAILEIRVGGRRIVFSGDLGQPGRPIVEDPTPIEAADVL
                     LVESTYGDRNHKSLVQTLDEFAYALTDTLSSRRGNVVIPSFAVGRTQDILCYIAQLQR
                     EQRVPPMNVYVDSPMALAATRITLKYAKLPDPGNVRFVESVEESMGLNSIRAGAVIIS
                     ASGMCDGGRIKHHLRYNLSRPECAIVFAGFQAQGTLGRRIVDGAKSVRIFGEPYPVRA
                     KVFTIGGLSSHADQAALMGWLGGFKRPPQQTWVVHGEPLSARALRDEIRRKGWNVQVP
                     SPGQVIEVD"
BASE COUNT         1368 a         2778 c         2620 g         1144 t
ORIGIN      
        1 ggcgcaggtc gcgatcgcgc cgcaggtagc ggtcgaacac catgcagata ctgcgaatca
       61 gcagcctccc cttcgcgctc accgtgagcc agccctcttc cagggcgacc agccccatcc
      121 gttggaactc ccgcagctcc tccagctcgg tcgcgaaata gcggtcgaag tccaggagat
      181 aggcgacctc gatcgattcc ttggagagcg cgaactggca catcagcgcc tggatgaccg
      241 cccggcgcac gaggtcatcg gcggtcaact cgatgccgcg catgatcggc agctcgccgc
      301 ggtcgagcgc gtcgtaatag ccttccagcg cccgctggtt ctggctgtag gtcgggccga
      361 tcgcgccgat cgcggacacg cccagcccca ccaggtcggc gttggcgagc acggagtagc
      421 cctggaaatt gcgatgcaag cgaccctgcc gctgcgccac ggcaagcgca tcgtcgggca
      481 gcgcgaagtg atccatgccg atgtagacat agcccgcggc ggtgagcagc cggatcgcca
      541 gttccagcaa ctggagcttg ccttccgacg aaggcaggtc gtcctcccga atgcgccgct
      601 gggacttgaa gagctgcggc aggtgcgcgt agttgtagag cgcgacccgt cccgggcggg
      661 ccgcgaccac ctgcgccagc gtcgtgtcga agctcgcggg cgactgcttc ggcaggccgt
      721 agatgaggtc cacgttgatc gaatcgaaac cggcccggcg cgccgcgtcg atcacggcga
      781 gcgtccgctc ccggggctga aggcggtgca ccgccgcctg caccgcgggg tcgaaatcct
      841 gtacgccgaa gctcacgcgg ttgaacccca tgtcgcgcag cgactccatc gccggcgcgt
      901 ccacggtgcg cggatcgacc tcgatcgaat attcgccgcg gggcgcgaaa tcgaactgtt
      961 ccgtcaaccg cccgaacagg gcccgcaggt cgcccatgtc gtggtaggtc ggcgtgcctc
     1021 cgccccagtg catctgcgcg acgcggggat cgtcccggaa cagcgcggcc tgcaggtcga
     1081 tttccctccc caggtaatcc aggtagcgcg cggccttgga cccgtcacgg gtgacgatct
     1141 tgttgcagcc gcaatagaag cagatgtcgc ggcagaacgg caggtggacg tagagcgcga
     1201 gcgggcgctg gacgccgccg acgttgcggc gcgccgccca catcgtgtag cgggtttcgt
     1261 cgaacgcttc gatgaagcgg tcggcggtgg ggtacgaggt atagcgcggc ccggtgcggt
     1321 cgagacggcg caccaggtcg gcatcgaatt cgatcgtcgg agaattcatg gttgccgggt
     1381 gtccggattc ggccagtatt gcagagcggc cgcggcgccc cttgatatag gtcaagccgg
     1441 agccgcgcgg gcgcggcaga ctttcccctg tgaaaacccc ttctcgcccg ccagcccttg
     1501 acatcggccg actgcgggtt gcctgttcgg cgtgcagcct gcgcgagctg tgcctgccgg
     1561 ccgggctcga ggcgccggaa atggcgaaaa tcgaccggct cgtcagccag cgccgccgcg
     1621 tccgcaaagg cgagcatctc taccacgcag gggcatcgct cgctgcgctc ttcgccatcc
     1681 gcgccggctt cttcaagagc tgcgttctgc acgaggatgg ccgcgagcag gtcgccggct
     1741 ttcacatgat gggagacctg ctcggaatgg acgcgatcgg gggcagccgc cacgcgagcg
     1801 atacggtggc cctggaggac agcgaggtct gcgagatgcc cttccccctg ctcgagcagc
     1861 tctcgcgcga ggtcccctcg ctgcaacagc agttgcaccg ggtcatgagc cgcgagatcg
     1921 tccgggaaca cggggtcatg ttgctgctcg gcagcatgaa ggcggaggag cgcctggcgg
     1981 cctttcttct caacctgtcg cagcgcttcg ccgcgcgcgg ctattccccg gtccagttca
     2041 acctgcgaat gacgcgccag gaaatcggca gctatctcgg cctgaagctc gagaccgtca
     2101 gccgggcgtt ttccgcgttc caggagcagg gactgatgac agtgcggcaa aggcaggtgc
     2161 acatcgtcga tctttccggc ctgaaagccg tgctcggcgc ggcgcgctga gccgcgggag
     2221 cccccctcac gcgcggccgc gcggccgcag ccacttgtag gcctcgtacc agagcacgcc
     2281 cgcgacgccg gccgcggccg ccagggccag gtagccggga ggcagcggcg cgaatcggaa
     2341 aagctcggca atcggcgtga cgtagatcga ggcggccagc gcaacgaggg tgccggtggt
     2401 gatccaccac agcgccggat tcggggaccg caacgcgtcg aggatcaggc ggtcgcggga
     2461 ccgcgttgcg tggatcatgg caaggttgcc gaaaacgatg gcagcaaatc cgaatgagcc
     2521 tgcctcgccg cccgtgagtc cgttgccgac cacccacccg tacgccaggc acacggcgag
     2581 cagcaccgag gcgccgagcg cgaggctcac gcccagcatc tgcaggctga acagcggctc
     2641 ccgcggatcg cgcggcgggc gcgtcatggc gccctcctcg ctcttttcgg cctcgaacac
     2701 gatcgagcac gccgggtcga tcacgaattc caggaacacc acgtgcaccg ggtagatgaa
     2761 caccggccag ccgaacacga gcggcaggaa ggacatgccg gcgatcggca cgtgaaccgc
     2821 gagcagatag cgcatcgcgt tgcggatgtt ctcgtaaatg cgccggccca ggcgcaccgc
     2881 gcccacgatc gactggaaat cgtcctccag cagcaccagc gcggaggcct cgcgcgcgac
     2941 gtccgtgccg cgccggccca tcgccacccc gatgtgcgcc gccttcagcg ccggggcatc
     3001 gttcacgccg tcgccggtca tcgccacgat ctcgccgtcg gtcttgtagg cggtgatcag
     3061 ctgcagcttc tgctccggcc ggatgcgcgc gaaaacattg actcgccgtg ccgcctcggc
     3121 aagcgcgggc gcgtccatcg cggagatctc ggtgccggtc agggcgccgg cgccggcatc
     3181 aatgccggcc tggcgcgcga tcgcaagcgc ggtgcccggg taatcaccgg taatcatcac
     3241 gacacggatc ccggcgccgc ggcacagcgc cacggcctct ggcactctcg cgcgcagcgg
     3301 atcggcgagc gcgacgaagc ccagccagcg aaaagcgtac tgcctcggat cagcgggcca
     3361 ttccgcgtcc gcccagccgg cctcggcgac agcgagcagc cgcaggccgc tctccgcagc
     3421 acgccgcatt tccgcgtccg ccgccgcgcg cgccgtctca tccagcgcgc acaattccat
     3481 cacggtttcg gccgcgccct tgacggccac gcggcgcccg cccgaaggcg agcgccatgc
     3541 gtggcacacg gcgagaaaat caggcgttaa gggataatcg cgctcgagcg tccaggacct
     3601 gcgcaactcg gcggcgtgcc cgcccgcggc ggcgaggatc gcccgctcca tgggatcgaa
     3661 cggttccagc tcgcacgcga gcgccgccgc ctcgatcacg tactcgggtg ccgcgtcggt
     3721 ggaaatcgcg tccaccagcg ccatgcggtt ctcggtcagc gtgccggtct tgtccacgca
     3781 cagcacggtg gccgcgccga gcgtttcgat tgccggcata cggcgcgtga gcacgccgtc
     3841 gcggccgatg cgccaggcgc cgagcgcgag aaacacggtc agcaccaccg gaaactcctc
     3901 cggcaggatc gcgatggcga gggtaatgcc ggcgaggatg cccgccagcc agtcaccccg
     3961 cgtcgcgacg tgatacgcgg cgagcagcag ggacagggca atcgcgaaga cggcaacggc
     4021 cttcacgatg cgcgcggttt ccgtctgcag ccgggtcttc tcgatatcga gcgcggccag
     4081 cgactggccg atcttcccga attcggtgcg cggacccgtg cccgtcacct ccgcgcgccc
     4141 ctgccctctt accacgaggg tgcccgagta aaccggcagg tccggccgct tttccaccgg
     4201 cagcgattct ccggtcagca gcgattcgtc caccatcagg tcgttgacgg aatccaggcg
     4261 cgcgtcggcc ggcacacggt cgccctccgc cagcaacacg agatcgcctc tcgccacctc
     4321 cgtgcccgcg atgcgcagcg gcgcgccgtc gcggatcacc agcgcgcgcg gactggaaag
     4381 atcgcgcagc gcttcgagcg cccgctcgga tttgcgctcc tgcgccaccg tgatggtcat
     4441 gaccacggcg atcgacgccg cgagcaccag cgcttcgcgc acgtcgccca gcagcaggta
     4501 gaccagggcg cctgccacga gcagcaaaaa catcggctcg cgcacgacct cgagcgcaat
     4561 ggcgagcgcg ccgcgccggc ccgcgtcggg caggcggttc ggcccctccg cgtcgagcct
     4621 gcgtctcgcc tccgccgagc tcaagcccgg ataggccacc ccgttcaacc gcgcctcatc
     4681 ggctcaggca cgcggcgcag cgccgtccgg tggccggccg cgcaggtaga tcacccagta
     4741 gaccagcgcc acgagcacgc tgccgcctat gatattgccg gcgatcaccg ggcccaggtt
     4801 gcccagcatg ccggccacgg tgatggcatc gccgccgggc gggagcgcga gaccgtgcca
     4861 cttcagcagc atcgccagcg ggaagaagta catgttggcg atcgagtgct cgaaaccggc
     4921 ggcgacgaaa gccgagatcg ggaacacgat ggcgatgaac ttgtccacga cgctgcgccc
     4981 ggccagcgcc atccagaccg ccatgcacac caggatgttg cacagaacgc cgagcaggaa
     5041 agcgcgcccg accggcagcg cggccttggt tgcggcgatc ttgaggtagg ccgctcccac
     5101 cgctccgccg ttcatctccc aatggcccga gaaaaagacg atcaccgcca gccctgccgc
     5161 gccggcgaaa ttggccacgc aaaccacgac ccagttgcgc agtacttcgc ccaggctgac
     5221 gcaaccgtcg gcccagccca tggcgaggag attgttgccg gtgaagagtt ccgcccccgc
     5281 caccacgacc agcagcaggc ccagcgagaa gcacacgccg cccgccaccc gggcggcggc
     5341 gaagccgata gaggcatcgc ttaccacgag cgtgaaatag agcgagccca ggccgatgaa
     5401 cgcgccggcg agcatgccga gcatccacag ggtgagcagc ggcaaccgcg ccttgatgac
     5461 gcagaagtcc ttgacgcgcc ctgcgatctc cctgggagag aatgagtcga agccgaacat
     5521 ttccatggat tgcctctttg aaggtccccg ccgctagtgt cccgagtcgc tagttcgttg
     5581 ataaatccat gatcttgtcg atgcggctgg cacgacaaaa gcgtcgaaat tggcgccaga
     5641 cgcgaagcaa accgcagcca ggttggacac ctggcgagga tttgcgacaa agtatggcgt
     5701 caattatcgg catcttttgt tcagcgaatt agcgattcag gacactagat gccgaacgcg
     5761 cgcagcacgg cgaaatagac caggatgctc aggatatcct gaatcacggt cgccaccggc
     5821 ccgctgccgt acgccggatc caggccccgg cgggcgaaca gccagggcag cgcaagcccg
     5881 atggccgcag cgatggtgcc ggcgatcact atcgagagcg acacagccgc cgccagccgc
     5941 gcattgccga aggcgaacca aatcggcagg aaagccagcg cgcccagcgt caagccgatc
     6001 agcatgccgg tgcgcagctc gaccgagagc aggtggccga tgcccgtccg cgtaagtgac
     6061 agtccccgta ccgcaatcgc ctcggtctgc gtcccgatgg catcggcgat ataaaccagc
     6121 gcgggcacga agaacgccac agccaccgtc ttctcgaggg tcgcttcgaa ggtcgccatc
     6181 gccgcagtgg cgaccgcgcc gccgcccagt ccgaccagca gccacggcag gcggtgacgg
     6241 gcgctgcgca tgggcggatc ctcgatcgcg tggcgcgcct gcaccgactc gcgccggatg
     6301 ccggccagga cgtgcaaatc ctcgacgtgc tcgcggcgca gcgcctggag cagcgcctgg
     6361 gcgggcatca cgccaagcag cctgccgtca tgatccacga ccggcaatcc gtcgacgccg
     6421 tgatggagcg cgaccgaggc cgcgacctcc tggtcgtcct cggcgtgcac gcgcggaaag
     6481 gccggatcca tgatctcggc gagcagcgcg ccgccattgg cagtgaacaa ccggcctgcg
     6541 ggaatcgcgc ccagcagcaa tcccgaaccg tccgtcacgc acaccggatc gacgctcgcc
     6601 accttttccg cggccagccg cgccaccatc gcggcgacgg tctcgtcgcg gtggccccgc
     6661 ccaaccgacg cgacgaggtg cgctccgacc gtttcgcgac gcatcagtcg acctcgatca
     6721 cctggccggg cgacggcacc tgcacattcc agcccttacg ccgtatttcg tcgcgcagcg
     6781 cgcgcgccga gagcggttct ccgtgcacga cccatgtctg ttgcggcggg cgcttgaagc
     6841 cgcccagcca gcccatgagg gcggcctggt cggcatgcga ggagagcccg cctatcgtga
     6901 acaccttggc gcgcaccgga tacggttcgc cgaagatgcg caccgacttc gcgccgtcca
     6961 cgatgcgccg gccgagcgtt ccctgcgcct ggaagccggc gaacacgatc gcgcattcgg
     7021 gacgggagag gttgtagcgc aggtggtgct tgatgcgccc gccgtcgcac atgccgctcg
     7081 ccgagatgat caccgcgccg gcgcggatcg agttgaggcc catggactcc tcgacgctct
     7141 cgacgaagcg gacgtttccc ggatcgggca gcttcgcgta cttgagcgtg atgcgggtcg
     7201 ccgccagcgc catgggcgaa tccacgtaga cgttcatcgg cggcacgcgc tgctcgcgct
     7261 gcagttgcgc aatgtagcag agaatgtcct gcgtgcgccc gaccgcgaac gaaggaatca
     7321 ccacgttgcc cctgcggctc gacagcgtgt cggtaagcgc gtaggcgaat tcgtcgagcg
     7381 tctgcaccag gctcttgtgg ttgcggtcgc cgtaggtcga ctcgacgagc agcacgtccg
     7441 ccgcttcgat cggggtcggg tcctccacga tcgggcgccc gggctgcccc aggtcgcccg
     7501 agaacacgat tctccggccg ccgacccgga tctcgaggat ggcagaaccc agaatatggc
     7561 cggcgtcgcg gaatacgcag cgcaccgatg ggtgcggccg cacttcgacg ccgtattcga
     7621 ccggctgcag ccgccgcagg ctcgcgcggg cctgcgggtc ggtgtaccgc ggcgcgccgc
     7681 cctcgcgttc cgcctcccgt tcctgcaggt gaccgctgtc gggcagcatg acgcccagca
     7741 ggtcgcaggt cgggcgcgtc gcgtagacgg cgccctcgaa gcctgccgcg accagcctgg
     7801 gcagcaggcc cgagtgatcg atgtgcgcgt gcgacaggat gcaggcgtcg atcgagttgg
     7861 cgttgaaggg caggttctta ttgcgatcgt acgcctcgct gcgcttgccc
//
LOCUS       DBNGSG010000506         5475 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1863353, whole genome shotgun sequence.
ACCESSION   DBNGSG010000506 DBNGSG010000000
VERSION     DBNGSG010000506.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5475)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5475)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5475
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1863353"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1205)
                     /locus_tag="AC158Q_13500"
     CDS             complement(<1..1205)
                     /locus_tag="AC158Q_13500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3340830.1"
                     /translation="MNQRDPRMAGAGSFVVIGKDVPRTDAVPKVTGAAQYVADLRFPG
                     MLHAAVLRSPHPHARIVSIDTSAAAAMPGVKAVATGEDTARRKWGAFRPDLYPLAIGK
                     VRYAGDEVAAVAALDPETARAAVDKILVQYEVLPAVLSLDQALAPGAALVHDDAAGNV
                     AHQFSFERGDVDAGFRAAEVVVEGTWESARQWHTALETIGCVARWDSDRVTMWCNTQT
                     PFLARGRYATALGLSEAQVRVIQTEVGGGFGGKSGDDNASVICALLARLSGRPVKLIH
                     TREEEFLASHPRMPMRYWVRLGFRKDGRIMAKEIKMWADNGAYTGKSQAILGAASVRH
                     DALYKYPCVRGNSTLVYTNLVPTGAFRGFGNPSADWAVEQAWDLAAEKLGIDVPELLR
                     MNAVEPGDVS"
     gene            complement(1202..1678)
                     /locus_tag="AC158Q_13505"
     CDS             complement(1202..1678)
                     /locus_tag="AC158Q_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010811673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3340831.1"
                     /translation="MLVKFTINGDPVEVNLKSGDRLLVDVLREDLNLTGTKHGCGIGA
                     CGTCTVIVDGYPIASCLQLAAMCDGADVRTIEGLAGTAALHPVQQAFVNKSALQCGIC
                     IPGHVMAACALLEKNPDPSDEEIRASADSVYCRCTGYVRILEAYQEAIALARKGKP"
     gene            complement(1942..2424)
                     /locus_tag="AC158Q_13510"
     CDS             complement(1942..2424)
                     /locus_tag="AC158Q_13510"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3340832.1"
                     /translation="MPAHRILALIALAAGLAAVSGPAISVEESGPADALAESNPDFAR
                     GRRAVESGDWDAAIKAFHAAERRDSRNADIQNMLGYAYRNAGRLEPAFKHYQRALQLD
                     SRHRGAHEYIGEAYLMAGNPAKAEEHLAILRKICPRVCEEHDDLRKKIAAYRAGNKQP
                     "
     gene            2592..3380
                     /locus_tag="AC158Q_13515"
     CDS             2592..3380
                     /locus_tag="AC158Q_13515"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3340833.1"
                     /translation="MLEVRGLKKEFQPLSGPPVVAIGEVSFRVEARQFVSIVGPSGCG
                     KSTLLQCVAGLMRPTAGTIILGGREVTGPPEGLILLFQEYNKSLMAWRTVLRNVCFGL
                     ENRPGMTRAEMEAEARHYIGLVGLKGFENQYPWELSGGMQQRVAIARALACKPQVLLM
                     DEPFGSLDALTRIELEDTLLRLWESLRSTILFVTHDIEEAVYLSDRVYVLSRRPSRMI
                     EDAAVDLPRPRNQLASREDARFIRLRHHTFELISKEMGGGFEVL"
     gene            3380..4153
                     /locus_tag="AC158Q_13520"
     CDS             3380..4153
                     /locus_tag="AC158Q_13520"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3340834.1"
                     /translation="MRRIPWSGLLVLAACLLAWEAWAYWRRDLAAYFPPASQVLATLG
                     QILGSGELTGHIIATLRRFAEGYFLAALLAVCAGLCLGLWRWLYNAFEPLIELLRPMP
                     SPATIPIAILFFGIGDEMKVAVTVYACSWPILLNTLDGVRSVDRVLLATADTFRLSPW
                     ARFWKVVLPAASPQIVTGLRVSLAIALILVTTSEMVVSNDGLGYYVLESQRTYQMPQM
                     YAAVVALGVIGYALNRLFVEIDGWAMAWHKGATRKEGIL"
     gene            4150..4923
                     /locus_tag="AC158Q_13525"
     CDS             4150..4923
                     /locus_tag="AC158Q_13525"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3340835.1"
                     /translation="MSWKDSRWTGVGVIVLLGLLWEAAGRAGWVHKLLFPPLSVILEK
                     LLEITVSGEILQQLGVSLWRAALGYALAATFAIALGIVMGYWRRAYEACEITIELARA
                     VPPPAVIPLAMVFFGIGDALKVFIIFFACSFPILVNTIDGVRNVDPVLIRTARTFGLS
                     RLQTVWKVILPAAGPTIMTGLRIATAIALILTVISEMIGATSGIGYFILGSQRTLHIA
                     NMYAGMLALALAGYAINRVFLLADGRLMAWHKGLTRREQ"
     gene            complement(4971..>5475)
                     /locus_tag="AC158Q_13530"
     CDS             complement(4971..>5475)
                     /locus_tag="AC158Q_13530"
                     /inference="COORDINATES: protein motif:HMM:NF020650.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3340836.1"
                     /translation="AHWIDSNGGDSTKVKWVEIPFPQMAPALVNKQIDAYGAVEPFVT
                     VPVLNKQARQLGRQLGAMAPRLIIASYFGSETWIGKNTAAVKGFVAALNRGIDAHNAN
                     PDEAKAVLAKHTGLKPELFKDMPLPAFEKKIVEADFQPMLDVALRYKFVARKFPIPEV
                     ISKHVRA"
BASE COUNT          819 a         1763 c         1856 g         1037 t
ORIGIN      
        1 gaaacgtcgc ccggctccac cgcgttcatg cgcagcagtt ccggcacgtc gatgccgagt
       61 ttttccgcgg ccaggtccca tgcctgttcc accgcccagt ccgcggaagg gttgccgaag
      121 ccgcggaacg cgccggtcgg caccaggttg gtgtacacga gcgtggagtt tccgcgcacg
      181 caggggtact tgtagagcgc gtcgtggcgc acgcttgccg cgcccaggat cgcctgcgat
      241 ttcccggtgt aggcgccgtt gtccgcccac atcttgattt ccttggccat gatgcggccg
      301 tccttgcgga agccgagccg cacccagtag cgcatcggca tgcgcgggtg gctcgcgagg
      361 aattcctcct cgcgggtgtg gatgagcttc acgggtcgcc cgctcagccg cgcgagcagc
      421 gcgcagatga cggaggcgtt gtcgtcgccg gatttgccgc cgaaaccgcc gccgacctcg
      481 gtctgaatca cgcgcacctg ggcttcggac agcccgagcg cggtggcata gcggccgcgc
      541 gcgagaaagg gggtctgcgt gttgcaccac atggtgacgc ggtcgctgtc ccagcgggcg
      601 acgcagccga tggtctcgag cgccgtgtgc cattggcgcg ccgattccca ggtgccctcg
      661 acgacgacct cggccgccct gaaacccgcg tcaacatcgc cgcgctcgaa gctgaactgg
      721 tgcgccacgt tgccggccgc gtcgtcgtgg accagcgcag cgcccggcgc aagcgcctgg
      781 tcgagcgaga gcaccgcggg caacacttcg tactgcacca gtattttgtc caccgccgcg
      841 cgcgccgtct ccggatcgag cgcggcaact gccgccacct cgtcgcccgc gtaacggacc
      901 ttgccgatcg cgagcgggta gaggtccggg cggaaggcgc cccatttgcg cctggcggta
      961 tcttcgccgg tggcgaccgc cttgacgccg ggcatggccg cggcggcgct cgtgtcgatc
     1021 gagacgatgc gtgcgtgcgg gtgcgggctc ctcagcaccg cggcgtgcag catgcccggg
     1081 aaacgcaggt cggcgacata ttgcgccgcg ccggtgacct tgggcactgc gtcggtgcgc
     1141 ggcacatcct tgccgatgac gacgaaactg ccggcgcccg ccatccgcgg atcgcgctga
     1201 ttcacggttt ccccttgcgg gctagcgcaa tcgcttcctg gtacgcctcg aggatgcgga
     1261 catagccggt gcaacggcag tagacgctgt cggcgctcgc gcggatttcc tcgtcgctgg
     1321 gatcggggtt cttctccagc agcgcgcagg ccgccatgac gtggccggga atgcagatgc
     1381 cgcactgcag ggcggacttg ttgacgaagg cctgctgcac cgggtgcagc gcggcggttc
     1441 ccgccagccc ttcgatcgtg cgaacgtcgg cgccatcgca catcgcagcg agttgcaggc
     1501 aggacgcgat gggatagccg tcgacgatca cggtgcaggt gccgcaggcg ccgatgccgc
     1561 aaccatgctt ggtgccggta aggttcaggt cttcccgcag cacgtcgacc agcaggcggt
     1621 cgcccgattt cagattgact tcgaccgggt cgccgttgat tgtgaatttg accagcacta
     1681 gctccccaat atcctgtcgc cggttctcgt ttcgttcgct gcgcgttgaa cggcgggcgc
     1741 ttcgtccgtt ccaacgcgcc ggattaaacc ggcacttcgt gtcccggcct gatgaattat
     1801 atatggcgtc gcgcgccgcg gcgcaggatc ctcaagccgg aaacaaggag agggaaatcg
     1861 tccccgccgt tacggggtag tgctgcgctg tcggcgacgg gatgctccgc cgcacggcga
     1921 gcctgcattg cgcaagcttt gtcacggctg cttgttgccg gcccggtacg ccgcgatctt
     1981 tttcctgagg tcgtcgtgct cttcgcacac gcgcgggcag atcttccgca ggattgcaag
     2041 atgttcctcg gccttggccg ggttcccggc catcaggtag gcctcgccga tgtactcgtg
     2101 cgccccgcgg tgacgcgagt cgagctgcag cgcgcgctgg tagtgcttga atgcgggctc
     2161 cagccggccg gcgttgcggt aggcgtagcc cagcatgttc tggatgtccg cattgcgcga
     2221 gtcgcgccgc tcggcggcat ggaacgcctt aatcgcagca tcccagtccc cggactcgac
     2281 tgcgcgccgt cccctggcga aatcgggatt gctttcggcc agggcgtccg ccgggccgga
     2341 ttcctctacg gatattgccg gcccggagac ggcggccagt ccggcggcaa gggcaatcaa
     2401 cgcaaggatg cggtgggcgg gcatgacgat actcctttcg aggtttgcgg cggtgatgcg
     2461 tgccggctgt gaagcgaaca ggcgatcccc atcggctatt cccgggaatt tccagcatgc
     2521 gcatcgatga ataatactgg caaaggcggc gcattgttgg cacgatacgc tgccacggga
     2581 aaggagcagg catgctggaa gtccgcgggc tcaagaaaga atttcagccg ttgtccggcc
     2641 cgcccgtggt cgccatcggt gaagtgagct tccgggtcga ggcgcggcaa ttcgtttcga
     2701 tcgtcgggcc gtcgggctgc ggaaaatcca cgctgctcca gtgcgtcgcc ggcctgatgc
     2761 gtccgaccgc cggcaccatc atcctcggcg ggcgcgaggt taccggcccc cctgaaggcc
     2821 tcatcctcct gttccaggag tacaacaagt cgctgatggc ctggcgcacg gtgctgagga
     2881 acgtctgctt cggccttgaa aacaggcccg gcatgacccg cgcggaaatg gaagccgagg
     2941 cgcgccatta catcgggttg gtcggcctga aaggcttcga gaaccagtac ccctgggagc
     3001 tttccggcgg aatgcagcag cgcgtcgcga tcgcccgcgc gctcgcgtgc aagccccagg
     3061 tgctgctgat ggacgaaccg ttcggctcgc tcgacgcgtt gacgcggatc gagctcgagg
     3121 acaccctgct gcgcctgtgg gaatcgctgc gctcgacgat cctgttcgtc actcatgaca
     3181 tcgaggaggc cgtctatctc tccgaccgcg tctacgtgct gtcgcgccgg ccttcacgga
     3241 tgatcgagga tgcggcggtg gacttgccgc gcccgcgcaa ccagctcgcg agccgagagg
     3301 atgcccgctt catccggctc cgtcatcaca ccttcgagct gatctcgaag gaaatgggcg
     3361 gcggcttcga ggttctttga tgcgccgcat accctggtcc ggcctcctgg tgctggcggc
     3421 ctgcctgctg gcctgggagg cgtgggcgta ctggcgccgc gacctggccg cgtattttcc
     3481 gccggcgtcc caggtgctgg cgacgctcgg ccagattctc ggcagcggcg agcttaccgg
     3541 gcacattatc gcaacgctga ggcggttcgc ggaaggatac tttcttgccg ccttgctcgc
     3601 ggtctgcgcg gggctctgtc tgggactgtg gcgctggttg tacaacgcgt tcgagccgct
     3661 gatcgagttg ttgcggccga tgccgtcgcc ggcaaccata cccatcgcca ttctcttttt
     3721 cggcatcggc gacgagatga aggtggcggt gacggtctac gcctgctcgt ggccgatact
     3781 gctcaataca ctggatggcg tgcgctcggt ggaccgggtg ctgctggcga ccgccgacac
     3841 ttttcggctc tcgccgtggg cgcggttctg gaaggtcgtg ctgccggcgg cctcgccgca
     3901 gatcgtgacg gggttgcggg tttcgctcgc catcgcgttg atcctcgtga ccacctccga
     3961 gatggtggtc tcgaacgacg ggctcggcta ttacgtgctc gagagccagc gcacttacca
     4021 gatgccgcag atgtacgcgg ccgtcgtcgc gctcggggtg atcggctacg cgttgaaccg
     4081 gctgtttgtc gagatcgacg gatgggccat ggcctggcac aagggggcga cgcgcaagga
     4141 ggggatactg tgagctggaa ggactcgcgc tggaccggcg ttggagtaat cgtgctgctc
     4201 gggctcctgt gggaggcggc gggccgggcc gggtgggtgc acaagctgct gtttccaccg
     4261 ctgagcgtca tcctggagaa actcctcgag atcaccgtct cgggcgagat cctgcagcaa
     4321 ctcggcgtga gcctgtggcg cgcggcgctc ggctatgcgc tcgccgcaac gttcgcgatc
     4381 gcgctgggga tcgtgatggg ttactggcgc cgcgcctacg aagcctgcga gatcaccatc
     4441 gagctcgcgc gcgcggtgcc cccgcccgcg gtgatcccgc tcgcgatggt gtttttcggc
     4501 atcggcgatg cgctgaaggt gttcatcatc ttcttcgcgt gctcgttccc gatcctggtg
     4561 aatacgatcg acggcgtgcg caacgtggac ccggtcctga ttcgcacggc tcgcaccttc
     4621 gggttgtcgc gactgcagac cgtctggaag gtgatcctgc ccgccgcggg gccgaccatc
     4681 atgaccgggc tgcgcatcgc caccgccatc gccctgatcc tcacggtgat atcggagatg
     4741 atcggggcga cgtcgggcat cggctatttc atcctgggca gccagcgcac cctgcatatc
     4801 gcgaacatgt atgcgggcat gctggccctg gcgctggcgg gttacgccat caaccgcgtg
     4861 ttcctgctcg ccgacggacg cctgatggca tggcataaag gactcacccg ccgcgaacag
     4921 tgacggaaac cgcccgcgac gggcagggcg gcggggtagc ggcgcccggc ttacgcgcgc
     4981 acgtgcttgg atatgacttc cgggatcggg aatttgcgcg cgacgaactt gtagcgcaat
     5041 gccacgtcga gcatcggctg gaagtcggcc tccacgattt tcttctcgaa cgccggcagc
     5101 ggcatgtcct tgaagagttc cggcttgagt ccggtgtgct tggccagcac ggccttcgcc
     5161 tcgtcgggat tggcgttgtg cgcatcgatg ccgcggttga gtgctgcgac gaatcccttc
     5221 acggcggcgg tattcttgcc gatccatgtt tcgctgccga agtaggacgc gatgataagg
     5281 cggggagcca tcgcgcccag ctggcggccg agctgccgcg cctgcttgtt cagcacgggc
     5341 accgtgacga agggctcgac cgcgccgtag gcgtcgatct gcttgtttac cagcgccggc
     5401 gccatctgcg ggaacgggat ttccacccat ttcaccttgg tcgagtctcc gccattcgag
     5461 tcgatccagt gcgcg
//
LOCUS       DBNGSG010000507         3944 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3561656, whole genome shotgun sequence.
ACCESSION   DBNGSG010000507 DBNGSG010000000
VERSION     DBNGSG010000507.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3561656"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..170)
                     /gene="lptM"
                     /locus_tag="AC158Q_13535"
                     /gene_synonym="yifL"
     CDS             complement(<1..170)
                     /gene="lptM"
                     /locus_tag="AC158Q_13535"
                     /gene_synonym="yifL"
                     /inference="COORDINATES: protein motif:HMM:NF047847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS translocon maturation chaperone LptM"
                     /protein_id="HIR3340837.1"
                     /translation="MEIAAKALSAHHSSVTAYRLPFAATILPMRALLPMIVCALLVAG
                     CGYKGPLYLPKP"
     gene            complement(171..908)
                     /locus_tag="AC158Q_13540"
     CDS             complement(171..908)
                     /locus_tag="AC158Q_13540"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR3340838.1"
                     /translation="MTPGVVLRSTLFALFQLVITPPYALVALATFPFAPLTRYRVISA
                     WARIMVAAAESLCGIRYRVIGAEHLPPPPFIVLSKHQSAWETLAFQVVVPPQVWVVKR
                     ELLWIPFFGWGLAMLAPIAIDRNAGPRALRQMLDQGRDRLARGFCIVIFPEGTRVAPG
                     ARREYQPGGAWLAVKTATPVVPVAHNSGEKWRRNAFLKYPGTITVSIGAPIETRGLTP
                     EEINRKVEDWIESEMRRLTSYKVSGEQ"
     gene            complement(905..1438)
                     /gene="gmhB"
                     /locus_tag="AC158Q_13545"
     CDS             complement(905..1438)
                     /gene="gmhB"
                     /locus_tag="AC158Q_13545"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HIR3340839.1"
                     /translation="MKLVILDRDGVINHDSADHIKNPEEWKPIPGSLDAIARLTQAGY
                     QVVVATNQSGVGRGLFEMATLNAIHDKMHRAVGLAGGRIDAVFYCPHAREANCRCRKP
                     KPGLYEDIGKRFGASLKSVPCIGDSQRDLEAAAAAGGQPILVLTGKGEATRREGKLPP
                     GTLVFKNLAEAVKSLIK"
     gene            complement(1522..3609)
                     /gene="glyS"
                     /locus_tag="AC158Q_13550"
     CDS             complement(1522..3609)
                     /gene="glyS"
                     /locus_tag="AC158Q_13550"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HIR3340840.1"
                     /translation="MPDKKTNKLGGADTLLVELLTEELPPKALKGLSQRFCDALVAGL
                     EGASLLSRPHDATPSCFATPRRLAVSIPGVLEKAADVRAEKTGPSVNASPDAVKGFAG
                     KCGVPVDSLVKHQTAKGEVWLARYTVTGAKLDDVLVQKVSEALKALPIPKLMRWGDGN
                     AQFARPAHGLMMLHGSRVIAGDVLGVSSSNKTLGHRFFSGSAVTLKHAGDYEDVLSGK
                     GKVITDFAVRRADITKQLEKAADGATLVLNDALLDEITAIVEAPAVYAGEFSPDFLAV
                     PEECLILSMQQHQKYVPLRDKKTGKLLPRFLFVSNIETKQPGEIIRGNERVLRARLAD
                     AKFFYDQDRKTRLEARVPRLASVVYHNKLGSQLERVERIQLLAGKIARELGADPLLAE
                     RAAWLSKADLLTDMVGEFPELQGVMGRYYALNDGEPKAVADAIGAHYRPRFAGDALPD
                     DPVSASVALAEKLDALAGLFGIGQVPTGDRDPFGLRRAALGVIRILVERNLPLPLNEL
                     VNSAFSSFNGKVGDAHTDIESFIYDRFSGYLKDQGYSTLEVESVISKRPVQINLVLRQ
                     IEAVRAFNRLPEAGSLAAANKRVVNILKQAEAKGESFANVGCDELKEPAEKALFEALA
                     TASQKATPLFKQGDFTGYLKTFAVLKAPVDAFFDSIMVMAEDPALRRSRLALLADLRN
                     EMNRVADISKLAT"
     gene            complement(3613..>3944)
                     /locus_tag="AC158Q_13555"
     CDS             complement(3613..>3944)
                     /locus_tag="AC158Q_13555"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HIR3340841.1"
                     /translation="QNEVEQSKYNFELADTEMLFQHFGQFESEAKRLIEARLVLPAYE
                     MVLKCSHTFNLLDARGAISVTERAAYIGRVRNLARAIAQAYFESRERLGFPMLKPRPA
                     AKKKVRA"
BASE COUNT          694 a         1206 c         1270 g          774 t
ORIGIN      
        1 ttcggcttgg gcaggtaaag cgggccttta tacccgcacc cggcaaccag cagggcgcag
       61 acgatcatcg ggagcagggc gcgcatgggc aggatggtag cagcaaacgg caaacggtaa
      121 gcagtgaccg atgagtgatg agcggataag gccttcgccg caatctccat tcactgctca
      181 ccgctgacct tgtatgaagt caggcgccgc atctcgcttt caatccagtc ctcgaccttg
      241 cggttgattt cctcgggggt caggccgcgc gtttcgatcg gggcgccgat gctcacggtg
      301 atcgtgcccg ggtacttgag aaacgcgttg cgccgccact tctcgccgga attatgggcg
      361 accggtacca cgggcgtcgc ggttttcacc gcaagccagg cgccgccggg ctggtactcg
      421 cggcgggctc cgggagccac tcgcgtgcct tcggggaaga tcacgatgca aaatccgcgg
      481 gcgaggcgat cgcggccctg gtcgagcatc tggcgcaatg cgcgagggcc ggcattgcga
      541 tcgatcgcga tcggggcgag catggcgagc ccccagccga agaacggtat ccagagcagt
      601 tcgcgcttca ccacccacac ctgcggcggg accaccactt ggaaggcgag ggtttcccat
      661 gccgactggt gcttcgagag cacgatgaac ggcggcggcg gcagatgttc cgcgccgatc
      721 acgcgataac ggatgccgca gaggctttcc gcggcagcga ccatgatccg ggcccaggcg
      781 gagatcacgc ggtagcgggt gagcggcgcg aaggggaaag tggcgagcgc gaccagcgcg
      841 taaggcgggg ttatgacgag ttggaacagc gcgaacagcg tggaccgcaa tacgacgccg
      901 ggcgtcattt aattagggac ttgaccgcct cggccaggtt cttgaacacc agcgtgccgg
      961 gcggcagctt gccctcccgc cgggtcgcct cgcctttgcc ggtgagcacc agtatcggct
     1021 ggccgccggc cgccgcagcg gcctcgaggt cgcgctgcga atcgccgatg cagggaacgc
     1081 tcttcaggct cgcgccgaag cgcttgccga tgtcctcgta aaggccgggc ttgggcttgc
     1141 ggcagcggca gttggcttcc ctcgcgtgcg ggcagtagaa cacggcatcg atgcgcccgc
     1201 cggccaggcc gacggcgcgg tgcatcttgt cgtggatggc gttcagcgtc gccatctcga
     1261 acaacccgcg acccacgccg gactggttgg tcgcgaccac cacctggtag cccgcctggg
     1321 tgagccgtgc gatcgcgtcg aggctcccgg gaatcggctt ccattcctcg gggttcttga
     1381 tgtggtcggc gctgtcgtgg ttgatgacgc cgtcgcggtc cagaatgacc agtttcatgg
     1441 tgaatggtga atggtgattg gtgaatggtc agaagcgcac ggactttgca tgttagtttc
     1501 ccgattcacg attcacggcc tttatgtggc aagcttggag atgtcggcga cgcggttcat
     1561 ctcgttgcgg agatcggcaa gcagcgcgag ccggctccgg cgcaaggcgg ggtcttccgc
     1621 catcaccatg atggaatcaa aaaaggcgtc gaccggagcc ttgagaacag caaatgtttt
     1681 caggtagccg gtgaaatctc cctgcttgaa caggggcgtc gctttctgcg aagcggttgc
     1741 gagcgcttcg aacagggcct tttccgcggg ttccttcaac tcatcgcacc ccacgtttgc
     1801 gaaggattct cccttggctt cggcctgctt cagtatattc acgacgcgtt tgttggctgc
     1861 cgccaggctc cctgcttccg gcagcctgtt aaaggcgcga actgcttcga tctggcgcag
     1921 aaccaggttg atctggacgg gccttttgct gatcaccgac tcgacttcca gcgtcgaata
     1981 gccttgatcc ttcaggtagc cggaaaacct gtcgtaaata aatgattcaa tatcggtgtg
     2041 agcgtccccg acctttccat tgaagctgct gaatgccgaa ttgaccagct cattgagcgg
     2101 caatggcaga ttgcgctcga cgaggattcg aatcacgccg agcgcggcgc ggcgcaggcc
     2161 gaaagggtcc cggtcgccgg tcggaacctg gccgatgccg aacaggcccg cgagcgcgtc
     2221 cagcttctcg gcgagcgcca ccgacgcgct gacgggatca tcggggaggg cgtctccggc
     2281 gaaccttggc cgataatgcg cgccgattgc atcagcgacg gctttcggct ccccgtcatt
     2341 cagggcgtag taacgaccca tcacgccctg cagttccggg aattccccga ccatgtcggt
     2401 gagcaggtcg gccttggaga gccatgccgc gcgctcggcg agcagggggt cggcgcccag
     2461 ttcgcgggca attttgcctg cgagaagctg gatgcgctcg acgcgctcca actggctgcc
     2521 aagcttgttg tgatagacga cgctggcaag ccgcggcacg cgtgcttcca ggcgggtctt
     2581 gcggtcctgg tcgtagaaga atttcgcgtc cgcgaggcgc gcgcgcagga cccgttcgtt
     2641 gccccggatg atctcgcctg gctgcttcgt ttcgatattg gatacaaaaa gaaaacgggg
     2701 gaggagtttc cccgttttct tgtcgcgcag cggcacgtat ttctggtgct gctgcatgga
     2761 caggatcagg cattcctccg gcaccgcgag gaaatcggga ctgaattctc ctgcgtatac
     2821 agcgggagcc tccacgatgg ctgtgatttc gtccagcagg gcgtcgttca gcacgagggt
     2881 cgcgccgtcg gcggccttct caagctgttt cgtgatatcg gcccgccgca cggcgaagtc
     2941 ggtgattact ttgcctttgc cggatagcac atcttcataa tcgccagcgt gcttcagggt
     3001 gaccgcgctg ccagaaaaaa agcggtgccc gagtgtcttg ttcgagctgc tgacccccag
     3061 cacatcaccg gcaatgactc gggagccgtg cagcatcatc aatccgtgcg ccggacgcgc
     3121 gaactgcgca ttgccgtcgc cccagcgcat cagctttggt atgggcagag ctttcagggc
     3181 ttcgctgacc ttctgcacga gaacgtcgtc cagctttgcc ccggttacgg tatagcgagc
     3241 cagccaaact tctcccttcg cggtctggtg cttcaccaga gaatcaacgg gcacgccaca
     3301 ctttccggcg aaacccttta ccgcatcggg gctcgcgttc accgacggcc ccgtcttctc
     3361 agcgcgcacg tcagccgcct tctcaagcac gccgggaatc gatacggcca ggcgtcgggg
     3421 agtcgcgaag caactcggtg tcgcgtcgtg tggccgggac agtaagctgg cgccctcaag
     3481 gcccgcgacc agcgcatcgc aaaaacgctg ggataagccc ttcagggctt tcggcggcag
     3541 ctcctcggtc agcagttcga ccagaagcgt gtctgcgccc cctagtttgt tcgttttctt
     3601 gtcgggcatc gctcaggcgc gcactttttt ctttgccgcc gggcgcggtt taagcatggg
     3661 gaagccgagc cgctcacgag actcgaagta agcctgcgcg attgcccgcg ccaggttgcg
     3721 cacgcggccg atgtaggcgg cgcgctccgt gactgaaatc gcgccgcgcg cgtcgagcag
     3781 gttgaaggtg tgcgagcatt tgagcaccat ttcgtacgcg ggcagcacga gccgcgcttc
     3841 gatcagccgc ttcgcctctg attcgaattg cccgaagtgc tggaacagca tctcggtgtc
     3901 ggcgagctcg aaattgtatt tcgactgctc gacctcgttc tggt
//
LOCUS       DBNGSG010000508         5735 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3052363, whole genome shotgun sequence.
ACCESSION   DBNGSG010000508 DBNGSG010000000
VERSION     DBNGSG010000508.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5735)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5735)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5735
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3052363"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..1916
                     /locus_tag="AC158Q_13560"
     CDS             207..1916
                     /locus_tag="AC158Q_13560"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HIR3340842.1"
                     /translation="MPETADGSVVGGVAGKRGGVTDDARQIALLKTGALQYAILTSAN
                     FSIIATDEKGIIQLFNAGAERMLGYTAAEVVNRISPSDIHDPQEVMARAEALSLELAT
                     PIAPGFEALAFKASRGIEDVYELTYICRDGSRFPAIVSITALRDEYRDIIGYLLIGTD
                     NSARKRVESELIEAMATADKANRAKTDFLSSMSHELRTPLNAILGFAQLMETGSPAPT
                     FSQKQNIDQILKGGWYLLELINEILDLALIESGRVTLSRESVSLVEVMLECRAMVEPQ
                     AQKRGIGMTFPRFDTPYFVKADRIRFKQVLINLLVNAIKYNKPGGEVAVELTPSPPDS
                     IRISVRDTGAGLTPEQLAQLFQPFNRLGKQAGAEEGPGIGLVVTKRLVELMRGTIGAD
                     SAVGVGSVFWIDLSLTAAPQIPTQEIEGVALARPPVPPGKPLRTLLYVEDNPANLDLV
                     EKLVERRPDLRLLGAADGNLGIEFARTFQPAVILMDINLPGINGIEAMKILRADPATA
                     HIPIIALSANAVPRDIKRGLDAGFFNYLTKPIKVNEFMDALDVALEFSKTASARAIEK
                     EQA"
     gene            1913..2749
                     /locus_tag="AC158Q_13565"
     CDS             1913..2749
                     /locus_tag="AC158Q_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3340843.1"
                     /translation="MRVTESDILDAGILIVDDQEINVSLLEQMLRESGYTRFSSTMKP
                     EEVCALHRRNRYDLILLDLQMPGMDGFQVIESLKTNDADGYLPVLVITAQPGHKLRAL
                     QAGAKDFVSKPFDLVEVRTRIHNMLEVRLLYKKLENYSKKLEQSVQDRTAELRESEAR
                     YRSLTELASDWYWEQDEVGKFIKVSGPVLEMLGIQVAPLVDDAAGGKAGAQPQGWNEA
                     ERAALQATIAARQPFLDFVFSRVNTDGSQQKFRVSGEPMFNRSSRYIGYRGIGVEITE
                     KH"
     gene            2774..3517
                     /locus_tag="AC158Q_13570"
     CDS             2774..3517
                     /locus_tag="AC158Q_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3340844.1"
                     /translation="MLSPHNPGQNRLLAALPAADLEALAAHLELVPLPLGEILYEPGG
                     QMQHAYFPTTAIVSLHYVMESGVSAETAGVGNEGVVGISLFMGGDTTPSSAVVQTAGH
                     AYRLAGSLLKKEFNRAGPMQSLLLRYTQALIAQMIQTAACNRHHSVEQQLCRWLLLTL
                     DRVSSQELIMTQELIASMLGVRREGITEAAGKLQRAGFIRYRRGHIAVIERSGLETRA
                     CECYAVVKKELGRLLFDVRHHQGIAAIAR"
     gene            complement(3581..4051)
                     /locus_tag="AC158Q_13575"
     CDS             complement(3581..4051)
                     /locus_tag="AC158Q_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3340845.1"
                     /translation="MRHSKLVTAAGAAVLLVSGLLVPALVPAWIPAASAAPIASNTRD
                     DAVVKTAGGVSYVSGGVGTDSLDRLNSLAGDFNLKLVFAMNSGSYVSAVGVVIADAAG
                     RTLLSATSDGPWFLTRLPAGNYQVVATFAGTSVRRQVAVGAVKLRTVDFRWASE"
     gene            4299..4508
                     /locus_tag="AC158Q_13580"
     CDS             4299..4508
                     /locus_tag="AC158Q_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HIR3340846.1"
                     /translation="MNWDRIEDNWNRIKGNVIEQWDDLTEGELVSRIQETYGTADDEA
                     ERELTDWQQRLSEINRAARPPQARS"
     gene            4929..5648
                     /locus_tag="AC158Q_13585"
     CDS             4929..5648
                     /locus_tag="AC158Q_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3340847.1"
                     /translation="MAGPAGATGATGPQGMQGPTGATGVTGRMVVGRAGVAGPAGAQG
                     AQGATGQTGSQGSSMAGPAGPTGRTGLAGVQGVTGSTGAEGPTTEGPTGPAGPAGATG
                     AQGATGYAGAQGSTELAGFAGPRGATGATGAQGSVGATGAQGSMIGGGNWSPYRDYSF
                     NVNSYDILSSDGNKAREIADYMNQNPSARIAIDGSNERRVARVRDALINAGVPSYKIQ
                     TGSFGDPQQRGDSRVAVLVSN"
BASE COUNT         1127 a         1831 c         1849 g          928 t
ORIGIN      
        1 ggccaggctg gcggatatcg ccgcgccagg ggccggctct gtgcgccagc gcacatacgt
       61 ccgtctggaa agccgccatg atccctgctg gccttgggag aggagcgcta ttgctgcggg
      121 agcgcatggc cgcccgcctt ccgtggcctg ccccatgaac gcagttcatt caccggatcg
      181 gtgcacgctt ttcacggaat caggacatgc ccgaaacagc agatggatcc gtcgtgggcg
      241 gcgtcgccgg caagcgtggc ggcgtcaccg atgacgcgcg ccagatagca ctgctgaaaa
      301 cgggcgccct gcagtacgcg atcctcacca gcgccaattt ctcgatcatc gccaccgacg
      361 agaaaggcat catccagctg ttcaatgccg gtgcggagcg catgctgggc tacacggcgg
      421 cggaagtcgt gaaccggata agcccgagcg atatccacga tccgcaggaa gtcatggcgc
      481 gcgccgaggc attgagcctc gagctcgcca cccccatcgc gccgggcttc gaggcgctgg
      541 ctttcaaggc ttcgcgcggg atcgaggacg tctatgaact gacctatatc tgcagggatg
      601 gcagccgttt tccggccatc gtgtcgatca cggcgctgcg cgacgagtac cgcgacatca
      661 tcggttacct gttgatcggc accgataatt ccgcgcgcaa gcgcgtcgaa tcggaactga
      721 tcgaggccat ggccaccgcg gacaaagcca accgcgcgaa aacggatttt ctctcgagca
      781 tgagccatga attgcgcacg ccgctgaatg cgattctcgg cttcgcccag ctcatggaaa
      841 ccggctcccc ggcgccgacg ttctcccaga agcagaacat cgaccagata ctcaaggggg
      901 gatggtacct gctggagctg atcaacgaga tcctcgatct cgcgctgatc gagtcgggca
      961 gggtgacgct gtcgcgggag tcggtctcgc tggtcgaggt catgctcgaa tgccgcgcca
     1021 tggtcgagcc gcaggcgcag aagcgcggca tcggcatgac gtttccgcgc ttcgacaccc
     1081 cttacttcgt caaagccgac cggatcaggt tcaagcaggt cctgatcaac ctgctggtca
     1141 acgccatcaa gtacaacaag ccgggaggcg aagtcgccgt ggagttgacc ccgagccctc
     1201 cggactccat acgtatcagc gtacgggaca ccggcgcggg gctgaccccg gaacaactgg
     1261 cgcagctttt ccagccgttc aatcgtctcg gcaagcaggc cggcgccgag gagggcccgg
     1321 gtatcggcct ggtggtgacc aagcggctgg ttgaactgat gcggggcacc atcggcgcgg
     1381 acagcgccgt cggggtggga agcgtgttct ggatcgactt gagcctgacc gccgcgccac
     1441 agatccccac ccaggaaatc gagggcgtgg cgctcgcgcg cccgccggta ccgccaggca
     1501 agccgctgcg cacgctactc tacgtggaag acaatcccgc caacctggat ctggtcgaga
     1561 aactcgttga gcgccgcccc gacctgcgcc tgctgggcgc ggcggacggc aatctcggca
     1621 tcgagttcgc gcgcaccttt cagccggcgg tgatcctgat ggacatcaac ctgcccggca
     1681 tcaacggcat cgaagcgatg aaaatcctgc gcgcggaccc ggcgaccgcg catattccca
     1741 tcatcgcgct gagcgccaac gcggtgcccc gcgacatcaa gaggggcctc gacgccggat
     1801 tcttcaacta tctcaccaag cccatcaagg tcaacgaatt catggacgcg ctcgacgtgg
     1861 cactggaatt ttcgaaaacc gcatcggctc gcgcaattga aaaggagcag gcatgagggt
     1921 taccgagtcc gatattctcg atgccggcat cctgatcgtc gacgaccagg agatcaatgt
     1981 cagcctgctc gagcagatgc tgcgcgagtc cggctacacc cgcttctcct cgaccatgaa
     2041 gcccgaggaa gtctgcgcgc tccaccgcag gaaccgctac gacctgatcc tgctcgacct
     2101 gcagatgccc ggcatggacg gattccaggt gatcgaatcc ctcaagacca acgatgcgga
     2161 cggctacctt ccggtgctcg tgatcaccgc ccagcccggc cacaagctgc gcgcgctgca
     2221 ggccggcgca aaggacttcg tcagcaagcc gttcgacctg gtcgaagtca ggacgcgcat
     2281 ccacaacatg ttggaagtgc ggctgctgta caagaaactc gagaattaca gcaagaagct
     2341 ggaacaatcg gtgcaggacc ggaccgccga gctgcgcgaa agcgaggccc gctatcgcag
     2401 cctgaccgag ctcgcctccg actggtactg ggagcaggac gaagtcggaa aattcatcaa
     2461 ggtttccggc ccggtgctgg agatgctcgg aatccaggtc gcccccctgg tggacgatgc
     2521 ggccggggga aaggcgggcg cccagccgca aggctggaac gaggcggagc gggcggcgct
     2581 gcaggcaacc atcgcggcgc ggcagccgtt cctggatttc gtcttcagcc gcgtcaatac
     2641 cgatggctcg cagcagaagt tccgggtcag cggcgagccg atgttcaacc ggtccagccg
     2701 ctatatcggt taccgcggaa tcggcgtgga aatcaccgaa aagcattaga gcgcaggatc
     2761 gagacggtca gcgatgttat cgccccataa tccgggccag aaccggctgc tcgccgccct
     2821 gcccgcggcg gatctcgagg ccctggccgc gcacctggaa ctggtcccgc tgccgctcgg
     2881 cgaaatcctc tacgagcccg gcgggcagat gcagcatgcc tattttccca ccaccgccat
     2941 cgtgtcgctg cactatgtca tggagtccgg cgtgtcggcg gaaaccgcgg gggtgggcaa
     3001 cgagggcgtg gtcggcattt cgctgttcat gggcggggac accacgccga gctcggccgt
     3061 ggtgcagacc gcgggccacg cctaccggct ggcgggcagc ctgctcaaga aggaattcaa
     3121 tcgcgccggc ccgatgcaga gcctgctgct gcgctacacc caggcgctga tcgcgcagat
     3181 gatccagacg gcggcctgca accggcacca ttcggtcgag cagcagttgt gccgctggct
     3241 gttgttgacc ctcgaccggg tctcctcgca ggagctgatc atgacccagg aattgattgc
     3301 cagcatgctc ggcgtgcgcc gcgaaggcat taccgaggcc gccgggaagc tgcagcgcgc
     3361 cggcttcatt cgctaccgcc gcggccatat cgcggtgatc gagcgctccg ggctggagac
     3421 ccgcgcgtgc gagtgctatg cggtggtcaa gaaggaattg ggacgcctgc tgttcgacgt
     3481 gcggcaccac cagggcattg ccgccatcgc ccggtaaccg tccccggccc gcgccctcat
     3541 gttcccggcc cgggtctgca tccatcgggc ccggcaggtt ctactcggag gcccaccgaa
     3601 aatcgaccgt cctgagtttt accgcgccga cggcgacctg gcgcctgacc gaagtgcccg
     3661 cgaaggttgc gacaacctga taattgcccg ccggtaaccg ggtcaggaac cacgggccat
     3721 ccgacgtggc gctcagaagc gtcctgcctg ccgcgtcggc gatgacgacc ccgacggcgc
     3781 tcacgtaaga gccggagttc atggcgaaca cgagtttgag attgaaatcg ccggcgagcg
     3841 aattcaaccg gtcgagcgaa tccgttccga ctcccccgga cacgtaagac acgccgccgg
     3901 cggtcttgac gaccgcgtcg tcgcgggtgt tgctcgcgat gggggcggca ctcgcagcgg
     3961 gaatccatgc gggaaccagc gcgggaacga gcaatccgct gaccagcaga acagccgctc
     4021 ctgccgctgt aaccagtttc gaatgtctca tgatgattcc tcttcaggtc aggtaatttc
     4081 ctgcaggcga ttatccgcaa atccgggacg atttctgtgc gacagcgtac agagcacgcc
     4141 agggcagact gcgcaggctg gaatttattg gccggacgtg cgccagcgca cggagcgact
     4201 cgggaaaggc gaccagcatc tctctgacgt gggccttttg cggggcggca atttccaaga
     4261 gggaaggcca gccgcccaat tgtgaaggaa aattgaaaat gaactgggat cgcatcgaag
     4321 acaactggaa cagaatcaag ggcaacgtca tcgagcaatg ggatgacctt accgagggcg
     4381 agctcgtcag ccggatccag gaaacctatg gaactgcgga tgacgaggcc gagcgcgaac
     4441 tcacggactg gcagcagcgc ttgagtgaga tcaatcgcgc cgcgagacct ccgcaggccc
     4501 gcagttgatc tacgggggtg cgttgccgta cagcgcttcc cggtccccga ctgggggttc
     4561 acttgccgag tacgcaggaa aaacggcgat atgagcgctg gcgtacggag acggcgggat
     4621 tatcagcgta gcttgcccat aagaatcgta tcccgcgcac tcgattccga cgcaaaggaa
     4681 tttgacaagt gcgcggttct gttacccaca ttaaaggaat cacgatgaga aattcgatgc
     4741 tgttcatgaa acgcccgctg ttgctcgcgg tcattgcgac gttcgccgtc gccggatgcg
     4801 cgacgaaaca acccgtcgcc cagaggtccg aacccgtcag ctacgtcggc ccggccggtc
     4861 ctgccggccc tactggcgcc acgggtgagc aaggtgctac tggatttacc ggcgcgcccg
     4921 gcgtagccat ggcaggtcct gccggcgcca ccggtgctac cggcccccaa ggcatgcaag
     4981 gcccgaccgg ggcaacgggc gtcactggca ggatggtggt tggccgtgcc ggcgtagcgg
     5041 gtcccgcagg cgcgcaaggt gcgcaaggcg ccaccgggca gaccggttcc caaggctcca
     5101 gcatggcagg tcccgccggc ccgaccggac gtaccggtct cgcaggcgtg caaggcgtaa
     5161 ccggaagcac gggtgccgaa ggcccgacta cggaaggtcc gaccggcccg gcaggtcctg
     5221 cgggcgcaac cggcgcgcaa ggcgctacgg gatatgcggg cgcccagggc agcacggaac
     5281 tggctggctt cgctggcccg aggggtgcta ccggggcgac cggtgcgcaa ggctcggtcg
     5341 gcgcgactgg cgcccagggc tcgatgatcg ggggcggcaa ctggagtccg taccgcgact
     5401 actcgttcaa cgtcaacagt tacgacatcc tgagctccga cggcaacaag gcgcgggaaa
     5461 tcgcggacta catgaatcag aatccgtccg cccggatcgc gatcgacggc tccaacgagc
     5521 gccgtgtcgc cagggttcgt gacgctttga tcaatgccgg cgtgccgtcc tacaagatcc
     5581 agaccggatc tttcggcgac ccgcagcaac ggggtgatag ccgcgtcgca gtgctggtca
     5641 gcaactgatc aggctttagc agttacagca gcagaacggc gcgatcgcga gatcgcgccg
     5701 ttcgcttgta cggggccgga aacgggagcg ttgcc
//
LOCUS       DBNGSG010000509         6046 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_676356, whole genome shotgun sequence.
ACCESSION   DBNGSG010000509 DBNGSG010000000
VERSION     DBNGSG010000509.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6046)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6046)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6046
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_676356"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..995)
                     /locus_tag="AC158Q_13590"
     CDS             complement(72..995)
                     /locus_tag="AC158Q_13590"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3340848.1"
                     /translation="MNSIKRIGFIGIGNMGAPMAGHLVKKGFDVTVYDIRPEVVKAFV
                     AQHGGKGAATLVDAAYGMDAVITMLPDDKVVRKAVLGDGSENCVAAGLARGTVVIDMS
                     TCNPTATRSLAEALKPRGITVVDAPVMGGVIFAKDATLDIMVGGEAEFVERVKPVLSA
                     IGRNLIACGGTGTAHAMKALANYINACALINAVEALTIGKRFGLDAKLMAEALIPMCA
                     GRNHPIEKKVIPHILTRRYGTGMAMGFIAKDVKIAVETAKSIGAFAPLGERVSELWSA
                     AVDQLGYDLDQTQVARYWEEASGVSLANDKK"
     gene            complement(1118..2236)
                     /locus_tag="AC158Q_13595"
     CDS             complement(1118..2236)
                     /locus_tag="AC158Q_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HIR3340849.1"
                     /translation="MSSYRPAEIGMPLSEVDTPSLLLDLDAFERNLDRMDASLAGKPI
                     KIRPHAKSHKCPEIALAQMKRGAVGVCCQKVSEAEAMVAGGVPDVLVANEAVGAAKLK
                     RLAALAQRARVAVCADDAGNVAALDEAARAAGIKLDVLVEVNVGANRCGVEPGEPALS
                     LAQVIARSKHLRFAGLQAYQGGAQHLRKVEERRAAIEAACDKVTRTTALLAKAGIACE
                     KVTGAGTGTYLFEAASGVYHELQAGSYIFMDVDYAKNDWTESGIPRFEHSLFVWTTLM
                     SRPVPDRGVVDAGLKASSVDSGMPRVADVEGAEYVKASDEHGVLKLDGRARLAVGDKL
                     KLIPGHCDPTVNLYDNYVCVRGGRVEAIWPITARGAVW"
     gene            2441..3439
                     /locus_tag="AC158Q_13600"
     CDS             2441..3439
                     /locus_tag="AC158Q_13600"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3340850.1"
                     /translation="MLISKSSRHWAAWCLAAGSIAAGPAGAQDWKPAQNVDIVVASGA
                     GGSSDRTARVVQKLLQANPAFPSISVTNRPGGGGTVAWTFLTQHPGNPHYIATFSPTM
                     VTNYILGVGKLNYTEFTPLSILLREYILVTVNADSPITSGKALIERVRADPTALSFAF
                     SAAPGNHNHIVIGMIFKAAGADPKKAKVVIQKSGGVGMTSILGGHIDVLVGAPANALP
                     HIMAGKARAIGLSAPQRQAGATAAIPTLREQGIDAVFYSWRGFVGAKDLAAPEIAFWD
                     QAFAKAVQGEDWKQDLSKNAWAEDLKSAAETRKHLDSEFALLKGMLGDLGVLPARQ"
     gene            complement(3566..4000)
                     /locus_tag="AC158Q_13605"
     CDS             complement(3566..4000)
                     /locus_tag="AC158Q_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG/HbpS family heme-binding protein"
                     /protein_id="HIR3340851.1"
                     /translation="MPQLTLKQANTIVEKALAKAREMKIKPLGVIVLDESGNIKSAQR
                     EDGASMFRLDVARGKAWAAVAMGSSSRALAKRAKDNPNFFLTLAATAEGKFLPQTGAV
                     LIRDKDGAIIGSAGASGGTGEEDEVCCVAGIEAAGLAADASA"
     gene            complement(4112..4612)
                     /locus_tag="AC158Q_13610"
     CDS             complement(4112..4612)
                     /locus_tag="AC158Q_13610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LeuD/DmdB family oxidoreductase small subunit"
                     /protein_id="HIR3340852.1"
                     /translation="MKFSAKVWKVGDNINTDLILPITAYYMTPQEQARMVFHANRPGW
                     VDQVRPGDILIGGRNFGMGSSRPAARSLKNAGLSCLVAHSINGLFYRNCINFAFPAIE
                     CPGIDEAFEEGEMAEVDFEAAVVRNATRGTTLTGRKTPPRLLDLLKAGGIYPLLENEG
                     LIAPKA"
     gene            complement(4792..>6046)
                     /locus_tag="AC158Q_13615"
     CDS             complement(4792..>6046)
                     /locus_tag="AC158Q_13615"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR02086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HIR3340853.1"
                     /translation="MGMTIAEKILAKKGGQAKVVPGDVVTVGVDTVILFDNNFMSSIW
                     REILKVHDPGRIVVTFDHRVPAPHQQAAAAQQTGRAFVRRFGIKRFHDVGYGQGISHQ
                     LVADHGYALPGSVLVCSDSHTCSAGVFNCLARGVGAPDIMYAATKGETWFRCGETVRY
                     ELAGKLSPAVTAKDAFLQIAGAHGEHATMNVEYGGPGMASLSISARKTLTTMSAELSA
                     EFATFEPDEVMLSWVRARNSGAFEPVYPDAGASYLAVRNVDLGSLEPLVAMPDTVVNN
                     SHPVKAAAGTRIDQAYIGSCANGTLDDLELAAQVVKGRRVAAGTRLVIVPASQTVYRE
                     AVKRGVIEALLDAGAMIGPPTCGACGGGHMGVLGPGEVCITSSTRNFEGRMGDPSSRV
                     YMASPATVAASAIRGVITDPREFLS"
BASE COUNT         1014 a         2042 c         1899 g         1091 t
ORIGIN      
        1 cgttactcgt tcttgacctt caccgtcaaa tggccattca ttgacaccag gaacgagcaa
       61 cgagtaacga gtcacttctt gtcgtttgcc aaagagacgc cgctcgcctc ttcccagtaa
      121 cgcgcgacct gcgtctggtc gagatcgtag ccgagctggt ccaccgccgc cgaccacagc
      181 tccgacacgc gctcgcccag cggcgcgaag gcgccgatgg atttcgcggt ttccaccgcg
      241 attttcacgt ccttggcgat gaagcccatc gccatgccgg tgccgtaccg gcgcgtcagt
      301 atatgcggga tcaccttctt ctcgatgggg tggttgcggc cggcgcacat cgggatcagc
      361 gcctccgcca tcagctttgc atccaacccg aaacgcttgc cgatggtcag cgcctcgacc
      421 gcgttgatca gcgcgcaggc gttgatatag ttggccagcg ccttcatcgc gtgcgccgtc
      481 ccggtgccgc cgcaggcgat gagattgcgg ccgatcgcgg acagtaccgg cttcacgcgc
      541 tcgacgaact ccgcttctcc gccaaccatg atatccagcg tcgcgtcctt cgcgaagatc
      601 acgccaccca tcaccggcgc gtccaccacg gtaatgccgc gcggcttgag cgcttcggcc
      661 aacgagcgcg tggccgtggg attgcaggtg ctcatgtcga tcacgaccgt gcccctggcc
      721 aggccggccg cgacgcagtt ctccgaacca tcgcccagca cggccttgcg cacgaccttg
      781 tcgtcgggaa gcatggtgat gacggcgtcc atgccgtacg cggcatccac cagggtcgcc
      841 gcgcccttcc cgccgtgctg tgcaacgaat gccttcacca cttccgggcg aatgtcgtag
      901 accgtgacgt cgaagccctt cttcaccaga tgcccggcca tgggagcacc catatttccg
      961 ataccgataa agccaatcct cttgatggaa ttcatagcgc gaatgcctct ctgactatcc
     1021 cggtgctcca agggatgagg gatgagggcg gagggatgaa gtaaacccgt agccaacaac
     1081 gcttctatcc tcatccctca taaatcatcc ctcatcccta ccaaaccgcc cctcgggcgg
     1141 tgatgggcca gatcgcttcg acgcgcccgc cgcgcacgca gacgtaattg tcgtagagat
     1201 tgaccgtcgg gtcgcagtgg ccggggataa gcttcagctt gtcgccaacg gccaggcgcg
     1261 cgcggccatc gagcttcagc acgccgtgct cgtcggatgc ttttacgtac tcggcgcctt
     1321 cgacgtccgc cacgcgcggc atgccggaat ccacgctgga ggccttgagc cccgcgtcaa
     1381 cgactccccg gtcggggacg gggcggctca tcagcgtggt ccagacgaag aggctgtgtt
     1441 cgaagcgcgg gatgccgctt tcggtccaat cgttcttcgc gtagtccacg tccatgaaga
     1501 tgtacgagcc tgcctgaagc tcgtgataga cgccgctcgc cgcctcgaac aaataggtgc
     1561 cggtgccggc gccggtcacc ttctcgcagg cgatgcccgc ctttgccagc agcgccgtcg
     1621 tccgcgtcac cttgtcgcac gcggcctcga tcgccgcgcg ccgctcctcc accttgcgca
     1681 ggtgctgcgc gccgccctgg taggcctgca gcccggcaaa gcgcaggtgc ttggagcggg
     1741 cgatgacctg cgccagcgaa agcgccggct cgcccggttc caccccgcag cggttggcgc
     1801 ccacgttcac ttccaccagc acatcgagct tgatgccggc ggcacgcgct gcctcgtcga
     1861 gcgccgcgac attgcccgcg tcgtcggcgc ataccgccac gcgcgcgcgc tgcgccagcg
     1921 ccgcaagccg cttgagcttc gccgcgccca ccgcctcgtt ggcgacgagg acatcgggga
     1981 ccccgcccgc caccatggcc tcggcttcgc tcaccttctg gcagcacacg cccaccgcgc
     2041 cgcgtttcat ctgcgcgagc gcgatctcgg ggcatttgtg gcttttcgcg tgcggacgga
     2101 tcttgatggg cttgcccgcg agcgaggcat ccatgcggtc gagattgcgc tcgaacgcgt
     2161 cgaggtcgag gagcagcgac ggcgtgtcca cttctgacag cggcatgccg atttcggcgg
     2221 gacgatacga actcatgagg gatgatggcg gtggggagga ctggtgattc tgtctgagtg
     2281 ccctgcgccg cgtcaatgcg cccgtcccgc gcggccgggt ttgccgatga gtgacagccc
     2341 ggcgattggt gtataggatt tgcctcagcg ggcaggcgcc ggcactcagc cggtcggata
     2401 ctgcggccct gcttcggttt attccaataa aggaggagtc atgctgatat ccaagtcatc
     2461 ccgccactgg gcggcctggt gcctggccgc cggctcgatt gcggcgggcc cggccggggc
     2521 gcaggactgg aagccggcgc agaacgtcga catcgttgtc gcgtccggcg cgggcggctc
     2581 gtccgaccgc acggcgcgcg tggtgcagaa actgctgcag gccaatcccg ctttccccag
     2641 catcagcgtc accaaccggc cgggcggcgg tggcacggtg gcgtggacgt tcctgaccca
     2701 gcaccccggg aatccccact acatcgccac gttctcgccg acgatggtga ccaattacat
     2761 cctcggcgtc ggcaagctga attacaccga gttcaccccg ctcagcatcc tgttgcgcga
     2821 atacatactg gtgacggtga atgccgactc cccgattact tcgggcaagg cgctgatcga
     2881 acgcgtccgg gcggacccga cggcgctgag cttcgccttc tcggcggccc cgggcaatca
     2941 caaccacatc gtcatcggca tgatcttcaa ggccgccggc gccgacccga agaaggcgaa
     3001 ggtcgtgatc cagaaatccg gcggggtcgg catgacctcg atcctgggcg gccacatcga
     3061 cgtcctggtc ggggcgcccg caaacgcgct cccgcacatc atggccggca aggcgcgcgc
     3121 gatcgggttg agcgcgccgc aacgccaggc gggggcgacg gccgccatac ccactctcag
     3181 ggagcaaggc atagacgcgg ttttctacag ctggcgcggc ttcgttggcg cgaaggacct
     3241 cgcggcgccg gaaatcgcgt tctgggacca ggctttcgcc aaagcggtcc agggagagga
     3301 ctggaagcag gacctctcca agaacgcatg ggcggaggac ctgaagagcg cggcggaaac
     3361 ccgcaagcac ctggacagcg agttcgcgct gctcaagggc atgctgggag atctcggcgt
     3421 ccttcccgcc aggcagtaac ggcgtgattc gtgattggtg attcgtgaat cgggaataaa
     3481 aaagggcggc caggccgccc tttttcatgc gccgcgccgg gcgcggccac ccaatgcttt
     3541 cgacttgcgc cttccgcctt gcctctcagg cggaggcatc cgccgcaagg cccgcggcct
     3601 cgatgccggc aacgcagcag acttcgtcct cttccccggt gccgccgctc gcgccggccg
     3661 agccgatgat cgcgccgtcc ttgtccctga tcagcacggc gccggtctgc ggcaggaact
     3721 tgccttcagc ggtcgcggca agcgttagga agaaattggg gttgtccttg gcgcgcttgg
     3781 cgagcgcccg gctggaagag cccatggcca ccgcggccca tgccttgccc ctggcgacgt
     3841 cgagtcggaa catgctcgcg ccgtcctcgc gttgcgcgga cttgatgttg ccggattcgt
     3901 cgagcacgat gacgccgagc ggcttgatct tcatctcgcg cgccttggcg agcgctttct
     3961 cgacgatggt gttggcctgc ttcaacgtga gctgcggcat gcattcctcc gtggtgggct
     4021 tgcggtatgg agcgccgcgg tgcggcgcga aaagagcggc gactatacaa gcgcccgcga
     4081 aaacctgtca aacgatgact tgccttgtct cttatgcctt cggagcgata agcccctcgt
     4141 tctcgagcag cggataaatc cccccggcct tcaacaggtc gagcagcctg ggcggcgtct
     4201 tgcgcccggt gagcgtcgtg ccgcgggtgg cgttgcgcac gacggccgcc tcgaaatcca
     4261 cttccgccat ttcgccttcc tcgaaagctt cgtcgattcc gggacattcg atcgcgggaa
     4321 aggcgaaatt gatgcaattg cggtaaaaca ggccgttgat ggaatgcgcg acgaggcagg
     4381 acaggccggc gttcttcagc gagcgcgccg ccggacggct cgagcccatg ccgaaattcc
     4441 tgcccccgat caggatatcg ccgggccgca cctggtccac ccagcccggc cggttggcgt
     4501 ggaacaccat gcgcgcctgc tcctgcggcg tcatgtagta cgccgtgatc ggcaggatga
     4561 gatcggtgtt gatgttgtcc ccgaccttcc agacttttgc gctgaacttc ataaaaacct
     4621 taaattagcc gccaagacgc caagaacgcc aaggaaatca aattaacaat tgaaaccaaa
     4681 ccgacaattc cactcttttc gccagcgctt accgaaattt aattcccttt actgtgattc
     4741 ttattattgt tgtgtccttc ttggcgttct tggcgtcttg gcggctattt tctagcttaa
     4801 aaactcgcgc ggatccgtga tgacgccgcg gatcgcggag gccgcgaccg tcgccggcga
     4861 ggccatgtac acccgcgagc tcgggtcgcc catgcggccc tcgaaattgc gcgtgctgga
     4921 ggtgatgcag acctctcccg gtccgagcac acccatgtgc ccgccgccgc aggcgccgca
     4981 ggtcggcggc ccgatcatcg cgccggcatc gagcagcgcc tcgatcacgc cgcgcttgac
     5041 cgcttcgcga tacaccgtct gcgaagccgg cacgatcacg agacgagtgc cggcggcgac
     5101 gcggcgcccc ttcaccacct gcgcggcgag ttccaggtcg tccagcgtgc cgttggcgca
     5161 ggagccgata taagcctggt ctatgcgagt gccggcggca gccttcaccg ggtgcgagtt
     5221 gttgacgacc gtatccggca tcgcgacgag cggctcaagg ctgccgagat cgacattgcg
     5281 cacggcaagg tagctcgcgc cggcgtccgg atagacgggc tcgaatgcgc cggaattgcg
     5341 ggcgcgcacc cacgacagca tgacttcgtc cggctcgaac gtcgcgaact cggcggacag
     5401 ctcggccgac atcgtggtga gcgtcttgcg cgcgctgatc gagaggctcg ccatgcccgg
     5461 tccgccgtac tcgacgttca tcgtggcgtg ttcgccgtgc gcgcccgcga tctggaggaa
     5521 ggcatccttg gccgtgaccg ccggtgaaag cttgcctgcc aactcgtagc gcaccgtctc
     5581 gccgcagcgg aaccaggtct cgcccttggt cgcggcgtac atgatgtccg gggccccgac
     5641 gccgcgcgcg aggcagttga atacgcccgc gctgcaggtg tgcgaatcgc tgcacaccag
     5701 caccgagccc ggcagcgcgt agccgtgatc ggcgaccagc tggtggctga tgccctggcc
     5761 gtagccgacg tcgtggaagc gcttgatgcc gaagcggcgc acgaacgcgc ggccggtctg
     5821 ctgggccgcg gccgcctgct ggtgcggtgc cggcacgcga tggtcgaacg tcacgacgat
     5881 gcgcccgggg tcgtgcacct tgaggatctc gcgccagatg ctggacatga agttgttgtc
     5941 gaacaggatc acagtgtcca cgcccaccgt caccacgtcg cccggcacga ccttcgcctg
     6001 gccgcccttc ttcgccagga tcttctcggc tatcgtcatg cccata
//
LOCUS       DBNGSG010000510         4542 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_846190, whole genome shotgun sequence.
ACCESSION   DBNGSG010000510 DBNGSG010000000
VERSION     DBNGSG010000510.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4542)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_846190"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="AC158Q_13620"
     CDS             complement(<1..595)
                     /locus_tag="AC158Q_13620"
                     /inference="COORDINATES: protein motif:HMM:NF028007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR3340854.1"
                     /translation="MAKLILTLEGSVIREIPIDKERIMIGRKPQNDIQIENLAVSGEH
                     ACIVTILADSFLEDLGSTNGTLVNGNQVKKHILQNNDVIEIGKYKMKYLVEATPAAAA
                     SPPPAEDFEKTMVMRSPKSVAAAPKPAASPPAAPPPAPKPAPAAPAPAAAAKPAAAPP
                     APAPVPATKAAPAPAAAAKPAAAPGEAHPLAALQILTG"
     gene            complement(616..1431)
                     /locus_tag="AC158Q_13625"
     CDS             complement(616..1431)
                     /locus_tag="AC158Q_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HIR3340855.1"
                     /translation="MIDLGSALEVAVATHQGMVRSHNEDSIAADVGAGLAVLADGMGG
                     YNAGEVASGIAVELIKTEMKKSLAGRRPAELSGQIAEQLITEHATQANAAIYHASQSQ
                     PQYSGMGTTLVVALWHNNHISVGHVGDSRLYRLRGGALEQVTRDHSLLQEQIDSGMIT
                     KEQARHSQNKNLVTRAVGIDPEVETEVHTYPVQAGDIYLMCSDGLNDMVTDEDIQLTL
                     ASLQANLPLAAQQLVQQACDNGGRDNVSVILVRVVKDFAARTGMLDKMKSWFR"
     gene            complement(1445..4039)
                     /locus_tag="AC158Q_13630"
     CDS             complement(1445..4039)
                     /locus_tag="AC158Q_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing
                     serine/threonine-protein kinase"
                     /protein_id="HIR3340856.1"
                     /translation="MRKLDFWRSDWFLGVLVALVVLAVSGRDLVQSLERKAYDLGMQA
                     ASRPASDKIAVIAIDDQSIANIGRWPWSREVHARMTDILSGARAKVIVNSAFFFEPQL
                     DPGLAYINKLIDLYKQLPPPAPGAPAAVELASFGTLLSEAEQRLNTDRTLSESFKRAG
                     NVLLPMVFQSLAEPQGAPDPKRPLPDYLARNSLQLAGGDPQTLAGDLPLYPIADLGAA
                     TLAIGHLNARNDVDGAVRTEPLLIRHHDKYFPSLALMAAAKSLNLQVTDIKPLMGEGV
                     RLGKLNIRTDSELRMHTFFYGDRDGRPAFSVDSFFDVLEKKVPAGKFQDKIVLIGATA
                     AGLGTSQVTPVSPSTAPVLTMAHSVSSLLNEHFFVVPWWGALVEKAVFLLVALYLILL
                     LPRLRAAAAGMLSLAIGVALVIAHFALMATQLMWLQLMSAVVLLAVGYAALTTKRFLV
                     TERGKEKSDIDSAQSNRMLGLAYQGQGQLDTAFDYFRKLPIDDGIMDLLYNLALDLES
                     KRQFNKAESVFRYMADYNPKFRDLDSRLNRAKAMSETVILGGAGGGHPGGTVILPGGG
                     VEKPMLGRYQVEKELGKGAMGVVYLGKDPKIGRVVAIKTMALSQEFDPDELKDVKERF
                     FREAETAGRLTHPNIVTIYDAGEEHDLAYIAMEFLKGKDLTPFIKPESLLPLPKVLSI
                     FARVAEALSYAHKNSVVHRDVKPANIMYEPESDTVKVTDFGIARITDSSKTKTGMVLG
                     TPSYMSPEQLSGQKIEGRSDLFSLGVAFYQMACGRLPFQGDSMAQLMFKIANEAHADI
                     RTHNQKLPACVAAIVNKALAKNPDQRYQDGEQMAKALRLCQQSLTSAARPAPVSASPA
                     PVTPATRA"
     gene            complement(4347..>4542)
                     /locus_tag="AC158Q_13635"
     CDS             complement(4347..>4542)
                     /locus_tag="AC158Q_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340857.1"
                     /translation="VFRRYADVVGKIGEVSSVTDASFKVTAQGGLIEVLRARGEDGKK
                     LSGGEYARSAGLAAGTILGN"
BASE COUNT          797 a         1434 c         1405 g          906 t
ORIGIN      
        1 cgccggtcag aatttgcagc gcagcgagcg ggtgcgcttc acccggagct gcggccggct
       61 tggcggcggc ggccggggcg ggcgccgcct tggtcgccgg gactggcgct ggagcgggcg
      121 gagccgcggc cggcttggcg gcggctgctg gtgctggcgc tgccggggcc ggcttgggcg
      181 ccggcggagg agccgccggg ggtgacgcgg ccggcttggg cgccgcggca accgacttgg
      241 gcgagcgcat caccatcgtc ttctcgaaat cctcagctgg aggcggactg gcggctgcgg
      301 cgggcgtggc ttcgaccaag tacttcatct tgtacttgcc gatctcgatt acatcgttgt
      361 tctgcaggat gtgcttcttg acctgattgc cgttgaccag cgtgccgttg gtgctgccca
      421 ggtcctcgag aaaggagtcg gcgagtatgg tgacgataca ggcatgctcg ccgctgaccg
      481 ccagattctc gatctggatg tcgttttgcg gcttgcgccc gatcatgatg cgttccttgt
      541 cgatcgggat ctcgcggatc accgaccctt ccagagtgag aatcaacttc gccatggtgt
      601 tatccccgcc ttccgctatc tgaaccagga cttcatttta tccagcatgc cggtgcgcgc
      661 ggcaaaatcc ttcacgaccc gcaccaggat caccgaaacg ttgtcgcgcc ccccgttatc
      721 gcacgcctgc tgcaccagct gctgcgctgc gagcggcaga ttggcttgca acgaggccag
      781 cgtcaattga atatcctcgt cggttaccat gtcgttcagc ccgtcggaac acatcaggta
      841 gatgtctccc gcctggaccg gatacgtgtg cacttccgtc tccacctcgg gatcgatacc
      901 gacggcacgc gtaaccaggt tcttgttctg ggaatgcctc gcctgctcct tggttatcat
      961 gccgctatcg atctgttcct gcaacaaaga atggtcccgc gtaacctgct cgagggcgcc
     1021 gccgcgcaac cggtacaacc gcgaatcgcc gacgtgtccc accgagatat ggttgttgtg
     1081 ccacagcgcg accaccagcg tcgtgcccat cccggaatac tgcggctggc tctgcgatgc
     1141 atggtagatc gccgcattgg cctgcgtggc gtgttccgtg atcagctgtt cggcaatctg
     1201 tccgctcagt tccgcggggc gcctgcctgc cagcgatttc ttcatctcgg tcttgatcag
     1261 ttcaacggcg atgccgctcg cgacttctcc cgcgttgtag ccgcccatcc cgtccgccag
     1321 caccgccagc ccggctccca cgtcggccgc aatgctgtcc tcgttgtgcg agcgcaccat
     1381 gccttgatgg gtggccaccg caacttcgag cgcgcttccg agatcgatca tgtcgattcg
     1441 ccccttatgc tcgcgtcgcc ggcgtcaccg gagcaggaga cgcggataca ggagccggtc
     1501 gtgccgccga cgtcaggctc tgctggcaaa ggcgcaacgc cttggccatc tgctcaccat
     1561 cctgataacg ctgatccgga ttcttggcga gggccttgtt tacaatcgcc gccacgcatg
     1621 ccggcagttt ctggttgtgt gtccggatat ccgcatgcgc ttcgttggcg atcttgaaca
     1681 tgagctgcgc catcgagtcg ccctgaaacg gcaaccggcc gcacgccatc tggtaaaaag
     1741 ccacgccaag cgaaaaaaga tccgagcggc cctcgatttt ctggcccgac aactgctcgg
     1801 gcgacatgta ggaaggcgtg ccaagaacca tgcccgtctt ggtcttggac gaatcggtta
     1861 ttcgcgcgat gccgaaatcg gtgactttga cggtatcgct ttccggctcg tacatgatgt
     1921 tggcgggctt gacgtcacgg tgcacgacgc tgttcttgtg cgcgtaggac agggcctccg
     1981 ccacccgcgc gaagatcgag agcaccttcg gcagcggcag caggctctcg ggcttgatga
     2041 acggagtcag gtccttgccc ttgaggaact ccatcgcgat ataggcaagg tcgtgttcct
     2101 cgccggcatc gtatatcgtc acgatattgg gatgggtgag gcggcctgcc gtctcggctt
     2161 cccggaaaaa tcgttccttc acgtccttca gttcgtcggg atcgaactcc tgcgacagcg
     2221 ccatggtctt gattgccacc acgcgcccga ttttggggtc cttgccgaga tagacgacgc
     2281 ccatcgcgcc cttgccgagt tccttctcga cctggtagcg gcccagcatg ggcttttcca
     2341 cgccccctcc cggcaggatg acggtgccgc cgggatgccc cccgcccgcg cctcccagga
     2401 tcactgtttc cgacatcgcc ttggcgcggt tcaggcggct atccaggtca cggaacttgg
     2461 ggttgtagtc ggccatataa cggaataccg attccgcctt gttgaactgg cgcttgctct
     2521 ctaagtcgag cgcgaggttg tagaggagat ccatgatgcc gtcatcgatg ggcagcttgc
     2581 ggaaataatc gaatgcggtg tcgagctggc cctgcccctg gtaggcgagc cccagcatgc
     2641 ggttgctctg cgcggaatcg atatcggact tctccttgcc gcgctcggtg acgaggaaac
     2701 gcttggtggt cagcgcggca tagccgaccg caagcaggac caccgcggac atcaactgca
     2761 gccacatcag ctgcgtggcc atcagcgcga agtgcgcgat gaccagcgca acgccgatag
     2821 ccaggctcag catgcccgca gcggccgccc tgaggcgcgg cagcagcagg atgagataca
     2881 gggcgacgag aaggaacacg gccttttcca ccagcgcgcc ccaccagggc accacgaaaa
     2941 aatgctcgtt gagcaggctc gacacggagt gggccatggt cagcaccggc gccgtggacg
     3001 gggagaccgg cgtgacctgc gaggtgccca gacccgccgc ggtggccccg atcagcacga
     3061 tcttgtcctg gaatttcccc gccgggactt tcttctccag cacgtcgaaa aacgagtcca
     3121 ccgaaaaggc gggcctgcca tcgcggtcgc cgtagaagaa agtgtgcata cgcagttcgg
     3181 aatcggtcct gatattgagc tttcccagtc gcacgccttc gcccatcaaa ggtttgatgt
     3241 cggtcacctg cagattgagg cttttcgcag cggccatcag ggcgagcgag gggaaatatt
     3301 tgtcgtgatg gcggatcagc agcggctcgg ttcgcaccgc gccgtccacg tcgttcctcg
     3361 cgttgaggtg gccgattgcc agcgtcgcgg cgccgagatc cgcgatcgga tagagcggca
     3421 gatcgcccgc aagcgtctgt gggtctccgc ccgccagctg caggctgttt ctcgccaggt
     3481 agtccggcag cggccgcttc ggatcgggcg cgccctgcgg ctcggcgagg gactggaata
     3541 ccatcggcag cagcacatta cccgcgcgct tgaagctctc cgacagcgtg cgatcagtat
     3601 tgagcctctg ctcggcctcg gacagcagcg tcccgaacga cgccagttcg acagccgcgg
     3661 gcgcgcccgg cgcaggcggc ggaagctgct tgtagagatc gatcagcttg ttgatgtaag
     3721 ccagtccggg gtccagttgg ggctcgaaga aaaaagcgct gttgacgatg accttggccc
     3781 tggcacctga caggatgtcg gtcatccggg cgtgtacctc gcgcgaccag ggccagcggc
     3841 cgatattggc gatgctttgg tcatcgatgg cgatgacggc gatcttgtcc gaagccgggc
     3901 gcgacgccgc ctgcatgccc aggtcatagg ctttgcgctc caggctctgg accaggtccc
     3961 ggcccgatac agccaggaca accagcgcca ccaagacccc gagaaaccag tcggatcgcc
     4021 aaaaatcgag ctttctcatc cctttgcctg cattttttta ttgttatcta gcgggttacc
     4081 gtcattcaac cgtcaattct acgatattgg ccgggaaagt atatgttttt ctggcaggag
     4141 gatgacgaaa cttcctatga atacaggata ttacggcgct gcgctggctg accggtcggc
     4201 aacggcgagc cgccctgact ggtggcggga cccattgacg ccgccatgcc agcaaggcga
     4261 ttgggaatcg accagcaaat caaagacgtg gcatgaactg ccgcgatgag atgaaaccgc
     4321 cgctgcagat gctgctgcga aagccgctag ttgccgagta tggtaccggc ggcaagccct
     4381 gctgagcgcg cgtattctcc tccggagagc tttttgccgt cttccccgcg cgcgcgcagc
     4441 acctcgatca ggccgccctg cgcggtgacc ttgaagctcg catcggtgac gctggagact
     4501 tcgccgatct tgccgaccac gtccgcatag cgccggaaca cg
//
LOCUS       DBNGSG010000511         2252 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_676790, whole genome shotgun sequence.
ACCESSION   DBNGSG010000511 DBNGSG010000000
VERSION     DBNGSG010000511.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_676790"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="AC158Q_13640"
     CDS             complement(<1..921)
                     /locus_tag="AC158Q_13640"
                     /inference="COORDINATES: protein motif:HMM:NF027838.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HIR3340858.1"
                     /translation="MTFFPRTLLWRSVLLIALLLVIAHVSWLEIFRVSQREPRARQIA
                     EQIVSVVNLTRAALITAQPSKRLELLEELSKEEGIQVYNGHPGERVTPLPDRPFLRLV
                     EEEVKRQLGSGTQLAISRDGVRGAWVSFRIDADDYWVFMPRSRLERTDPLRWVGWGAL
                     VLVLALLGAFLIVARINRPLRELTRAADEIGHGKTPPPVSETGPAEIRTLAHAFNQMS
                     ADLKRLDDERALLLAGVSHDLRTPLSRIRLGLEMMDERGDAALKAGLVQDIDDIDAAI
                     SQFLDFARVTEGEAVVHDGDLNAIARELRDR"
     gene            complement(1003..1740)
                     /gene="ompR"
                     /locus_tag="AC158Q_13645"
     CDS             complement(1003..1740)
                     /gene="ompR"
                     /locus_tag="AC158Q_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmolarity response regulator transcription
                     factor OmpR"
                     /protein_id="HIR3340859.1"
                     /translation="MTEPKTRILVVDDDARLRDLLNRYLNEQGYAVRVVPDAGEMNRY
                     LARERYDLMILDLMLPGEDGLSVCRRLRGGGENLPIIMLTAKGDDVDRIVGLEVGADD
                     YLPKPFNPRELLARIQAVLRRRPPVAPPGAPATEPQMVEFGSFRFNLAARSLAKEGQD
                     VPLTTGEFALLKVLVQHPRAPLSRDKLMELARGREFGAFDRSIDVQVSRLRKLIEPDP
                     AKPVFIQTVWGFGYVFIPDGKSDGGDK"
     gene            1953..2228
                     /locus_tag="AC158Q_13650"
     CDS             1953..2228
                     /locus_tag="AC158Q_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3340860.1"
                     /translation="MSKKLIGGLAVAVALGMAAPAWANGGHGHRHGNGHAYGHAKHWQ
                     KHHVRQHYVVREVYRPVPVYPRAVYPVYPVAAPGIHVVIPSIFIPFH"
BASE COUNT          386 a          737 c          759 g          370 t
ORIGIN      
        1 acggtcgcgc agctcgcgcg cgatggcatt gagatcgcca tcgtgaacga cggcctcgcc
       61 ctccgtaaca cgcgcgaaat cgaggaactg gctgatcgcg gcgtcgatgt catcgatgtc
      121 ctggaccagg cctgctttca gcgccgcgtc gcccctctcg tccatcatct ccaggcccag
      181 gcggatgcgg gaaagcggcg tgcgcaggtc gtgggacacg ccggcgagca gcagcgcgcg
      241 ctcgtcatcc aggcgtttca ggtccgcgct catctggttg aacgcgtggg cgagtgtgcg
      301 aatttccgcc ggcccggtct cggagacggg cggcggcgtt ttgccgtggc cgatctcgtc
      361 cgccgcgcgg gtgagctccc gcagcgggcg gttgatacgc gcgacgatga gaaaggcgcc
      421 gaggagcgcg agcaccagca ccagcgcccc ccagccgacc cagcgcaggg gatcggtgcg
      481 ctccagccgc gagcgcggca tgaacaccca gtagtcgtcc gcgtcgatgc gaaaactgac
      541 ccacgcgccg cgcacgccgt cgcggctgat ggcaagctgc gtcccgctac cgagctgccg
      601 cttgacctcc tcctccacca ggcgcaggaa cggccggtcg ggaagcggcg tgacgcgctc
      661 gccgggatgt ccgttgtaga cctgtatgcc ttcttccttg gacagctcct cgagcagctc
      721 caggcgcttc gagggttgcg ccgtgatcag cgcggcgcgc gtgagattga ccacgctcac
      781 gatctgctcc gcgatctggc gcgcgcgcgg ctcgcgctgg ctgacccgga aaatctccag
      841 ccaggagacg tgcgcgatca cgagcagcaa cgcgatcagc agcacgctgc gccacagcag
      901 cgttcgcggg aagaaagtca tgaggcgtga tgagtgacaa gtgacgggtg acaggagccg
      961 gttttccgcg ctgcgcgcgg ggcccttatc acttatcacc tgtcacttat cacccccatc
     1021 gctcttgcca tcgggaatga agacgtagcc gaatccccac accgtctgga tgaacaccgg
     1081 cttggcgggg tcgggctcga tcagctttcg caggcgcgag acctgcacgt cgatcgagcg
     1141 gtcgaaggcg ccgaactcgc gcccgcgagc gagttccatc agcttgtcgc gcgagagcgg
     1201 cgcgcgcggg tgctgcacca gcaccttgag cagcgcgaac tcgccggtgg tgagcgggac
     1261 gtcctggcct tccttcgcca ggctgcgcgc ggcgagattg aaccggaagg aaccgaattc
     1321 caccatctgc ggctcggtgg cgggggcgcc gggtggagca accggcgggc gcctccgcag
     1381 cacggcctgg atgcgagcga gcagctccct cggattgaac ggcttgggca ggtaatcgtc
     1441 ggcgccgacc tcgagcccga cgatgcggtc cacgtcgtcg cccttggccg tcaacatgat
     1501 gatcggcagg ttttccccgc cgccgcgcag gcgccggcac acggacagcc cgtcctcgcc
     1561 cggcagcatc aggtcgagga tcatcaggtc atagcgctcg cgcgcgaggt agcgattcat
     1621 ctcgccggca tcgggcacca cccgcaccgc gtagccttgc tcgttgaggt agcggttcag
     1681 caggtcgcgc aatcgggcat cgtcgtcgac gaccaggatt cgtgtcttgg gctcggtcat
     1741 gggcgcatcc tatccatttg ggcccggcgc ttccagttca ttgtaaagat ccgttaccgg
     1801 cgggcttcct gaaacaaatt gttgcaattg acccggagcc gggccccccg gcgcgcgtca
     1861 aaccccgtac gatggcgcat cgcctgaacg ggcggtgacg cacaacaggt ataacggtgc
     1921 atgccagggg acacttggaa aaggagtcaa caatgagcaa gaaactgatc ggcggactgg
     1981 cggtagcggt ggcgctgggc atggcggctc cggcctgggc gaatggcgga cacggtcatc
     2041 gccacgggaa tggccacgcc tacgggcacg ccaagcattg gcagaaacac cacgttcgcc
     2101 agcactacgt cgtgcgcgag gtgtaccggc cggtgccggt gtatccgagg gccgtgtacc
     2161 cggtttatcc ggtcgcggca cccggaatac acgttgtgat tcccagcatc ttcatcccct
     2221 ttcattaaag ggggcgtggt cggcgggggc gg
//
LOCUS       DBNGSG010000512         6526 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_3054759, whole genome shotgun sequence.
ACCESSION   DBNGSG010000512 DBNGSG010000000
VERSION     DBNGSG010000512.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6526)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6526)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6526
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_3054759"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /gene="pgsA"
                     /locus_tag="AC158Q_13655"
     CDS             complement(<1..365)
                     /gene="pgsA"
                     /locus_tag="AC158Q_13655"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HIR3340861.1"
                     /translation="MPLNLPNLLTWLRIVMIPLFVGIFYFEVSWVSEPNQNLVATIIF
                     TVAAITDWLDGWLARRLNQMSAFGAFLDPVADKLMVAAALITLVQLGRVDAIIALIII
                     GREITISALREWMAQIGAG"
     gene            complement(375..2177)
                     /gene="uvrC"
                     /locus_tag="AC158Q_13660"
     CDS             complement(375..2177)
                     /gene="uvrC"
                     /locus_tag="AC158Q_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HIR3340862.1"
                     /translation="MEFDPASIVSGLPHMPGVYRMLNSAGEVLYVGKALDLRKRVASY
                     FQKAGGLSPRIQLMLGQVAALETTVTRSEAEALLLESNLIKSLSPRYNILFRDDKTYP
                     YLVVTGNAYPRLGFHRGALDKRHRYYGPFPNAGAVRESIQLLQKVFRIRTCEDTVFAN
                     RSRPCLLHQIRRCTAPCVGLIRAEDYAEDVHGADLFLSGKEDEVIERQVARMNDASAR
                     MSYEEAAVFRDRIAALRKVRERQFVSGEGGDTDVIACGRAAGVVCVNLAMIRGGRHLG
                     DKNFFPRNADDCDAGQILEAFVQQHYLQHGVPPHIVAGGELDVPPLEQLLSERAGHEV
                     KIHIHPGGERKAWLDMAQRNAEIGAGQALGLRTTQESRLVALQQALELPETARRIECF
                     DVSHTMGEAAVASCVVYDKSALQKSEYRRFNITGIEPGDDYGAMREVLTRRYRKIVTG
                     GGRVPDLVLIDGGKGQLSVAMEVFAELGMSDVALVAVAKGEERRPGQEQLLATGRSAP
                     LILGAEHPALHLVQQIRDEAHRFAIQGHRARRGKARNSSTLEKIPGIGSKRRQRLLAR
                     FGGLRGLLAASVEELAQVEGISLVLAEKIYHELH"
     gene            complement(2167..2781)
                     /locus_tag="AC158Q_13665"
     CDS             complement(2167..2781)
                     /locus_tag="AC158Q_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR3340863.1"
                     /translation="MARHLDRVRCEYPDYHARPVACFGPARAQLLIVGLAPGMHGANR
                     TGRPFTGDHAGILLYDTLHRYGYADSPVSISATDGLRLKNCRITNAVKCLPPENRPMP
                     GEIRHCNDYLASEIHSMPDNAAILALGLIAHHAVLMACGLKPSTCGFRHGARHALPRG
                     QALYDSYHCSRYNTQTKRLTPEMFRNVFDAIGGYLISISSRRGI"
     gene            2874..3440
                     /gene="efp"
                     /locus_tag="AC158Q_13670"
     CDS             2874..3440
                     /gene="efp"
                     /locus_tag="AC158Q_13670"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIR3340864.1"
                     /translation="MAKVLATEIRAGNLIEWDKRVWRVLKSYHVHVGGRGGAFMQVEM
                     KDIESGTKRNERIRTEDKIERAFVDQRDMQYLYSDASGYIFMDKESFEQLTLPADFLE
                     GQAGYLMPNADVQVNFHNDRPIGVQIPSTVTMTVVETEPNLKGATATGSFKPAKTETG
                     VTVLVPQFVAEGEKIKVNTDTGDYIERA"
     gene            complement(3444..4367)
                     /locus_tag="AC158Q_13675"
     CDS             complement(3444..4367)
                     /locus_tag="AC158Q_13675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR3340865.1"
                     /translation="MSAHHTHRREPGRPAFGIALAITAGYAVIELAGGLWTGSLALLS
                     DAGHMFSDALALGLAAIAARLAQRPSGQRHTYGWERAEVIGALVNSVLMLAIVLLLVV
                     EAVSRLRAPGPVAAAAMMLIALAGLLVNATVIWVMSRGEPGLNSRAAMLHVMSDLVSS
                     FAALVAGAVIYMTGWLPIDPILSLVIAGLILFSTLRLLRDTLHVLMEGVPPALELGEI
                     GRGLAGLPGVVSVHDLHVWSIVPGRIALSAHIELVEMREWPAILAAARVLLHERHGIE
                     HITLQPEIHEAPGRRQTSVIQLWPRGHRPRG"
     gene            complement(4364..5398)
                     /gene="nagZ"
                     /locus_tag="AC158Q_13680"
     CDS             complement(4364..5398)
                     /gene="nagZ"
                     /locus_tag="AC158Q_13680"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HIR3340866.1"
                     /translation="MLDVAGTALTADDRRRLLHPLVGGVVLFTRNYSDSPQLMRLTDD
                     IHALRNPPLIIAVDHEGGRVQRFREGFTELPPMRELGRLWDANPQRARVLAGEVAFVL
                     GAELRAHGVDLSFAPVLDVDYGNSSVIGDRALHSDPHAVVELARGLFQGLRRAGMAGV
                     GKHFPGHGHVRGDSHHEVPVDDRNFEEIEASDLMPFRSLIDAGLGGVMPAHVIYPRVD
                     SRPAGFSAVWLKQVLRGTLGFNGVVFSDDLSMEGASIVGGVVDRANAALAAGCDMALL
                     CNNPAGADQLLEGLTYRMPAVSLARLARLHGRPRAEGMVKLREDAHYSRSLHAIAGIG
                     LRDGELPLSS"
     gene            complement(5428..5805)
                     /gene="acpS"
                     /locus_tag="AC158Q_13685"
     CDS             complement(5428..5805)
                     /gene="acpS"
                     /locus_tag="AC158Q_13685"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HIR3340867.1"
                     /translation="MIYGIGIDVVEPQRISRLLEKYGERFARRVLTQLEWAGYLKTAK
                     PVLFLANRFAAKEAFSKAMGTGFRYPVTLQCISVVQNAAGKPGFTFHPRLEELVNGLG
                     IASHHLTITDEQSLACACVVLEK"
     gene            complement(5802..6524)
                     /gene="pdxJ"
                     /locus_tag="AC158Q_13690"
     CDS             complement(5802..6524)
                     /gene="pdxJ"
                     /locus_tag="AC158Q_13690"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018441591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HIR3340868.1"
                     /translation="MILLGVNIDHVATLRQARGTDYPSPLEAALAAEGAGADYITLHL
                     REDRRHIQDRDVLALRESLGTRMNLEAAVTPEMIAFARRVRPHDVCLVPERRAELTTE
                     GGLDAAVKLKQVERACRDLGAAGIRVSLFIDADRKQIDAALKAGAPVIELHTGSYAGA
                     RSASQQRKALDRIRAATEYATGKGLHVNAGHGLNYVNVRPIAGIAHMRELNIGHAIVA
                     RSVFVGIAQAVREMKALMGSAQ"
BASE COUNT         1246 a         2118 c         1989 g         1173 t
ORIGIN      
        1 ttgcccgccc cgatctgggc catccattcc cgcaaggccg agatcgtgat ttcgcgtccg
       61 atgatgatga gggcgatgat tgcatccacg cgcccgagct gcaccagcgt gatcagggcg
      121 gccgccacca tcaatttgtc ggcgaccggg tccaggaatg ccccgaacgc cgacatctga
      181 ttcaggcggc gcgcgagcca gccgtcgagc caatcggtga tggccgcgac agtgaagatg
      241 atggtcgcca ccaggttctg attcggctcc gatacccagc tcacctcgaa atagaaaatc
      301 ccgacgaaca gcgggatcat cacgatccgc agccaggtga ggagattggg caggttaaga
      361 ggcatcaggg gcctttaatg gagttcatga taaatctttt cggcaagcac gaggctgatg
      421 ccttcgacct gggcaagctc ctccacgctg gcggcgagca gcccgcgcag gccgccgaat
      481 cgcgccagca gccgttggcg gcgttttgag ccgatacccg ggatcttctc cagcgtcgaa
      541 ctgttacggg ctttgccgcg gcgcgcgcga tgtccctgta tggcaaagcg gtgcgcctca
      601 tcacggattt gctggaccag atgaagggcc gggtgctccg cccccagaat cagcggtgcc
      661 gagcgcccgg tggcaagcaa ctgttcctgc cccggtcggc gttcctcgcc cttggcgact
      721 gccaccagcg ccacgtcgct catgccgagt tccgcgaaga cttccattgc aactgacagc
      781 tgccccttgc cgccgtcgat caggaccagg tccggcaccc tgcccccccc ggtgacgatc
      841 ttgcgataac ggcgggtcag aacctcgcgc atggcgccgt agtcgtctcc cggctcaatg
      901 ccggtgatat tgaaccggcg atattcgctc ttctgcagcg ccgacttgtc gtataccacg
      961 caggaggcga cggcggcctc tcccatggta tggctgacgt cgaaacattc gatgcgtcgc
     1021 gccgtctccg gcagctccag cgcctgttgc agcgcgacca gccgtgactc ctgggtcgtc
     1081 cgcaatccca gcgcctgtcc ggcgccgatc tcggcgttgc gctgcgccat gtcgagccat
     1141 gccttgcgct cgccgcccgg gtggatatgg attttcacct cgtgaccggc gcgttcagat
     1201 aacagctgtt ccagcggcgg cacatccagc tcgccgccgg ccacgatgtg tggcggcacg
     1261 ccgtgctgca ggtagtgctg ctgaacgaaa gcttcaagga tctggcctgc atcgcaatca
     1321 tcggcattgc gcgggaaaaa attcttgtct ccgaggtggc ggccgccccg gatcatcgcc
     1381 agattgacgc agacgacacc ggccgctcgc ccgcacgcga ttacgtcggt atcgccgcct
     1441 tctccgctca cgaactgccg ttccctcacc ttgcgcaacg ccgcgatacg gtcgcggaac
     1501 acagcggctt cttcgtagct catgcgggcg gacgcgtcat tcatgcgcgc cacctgtcgc
     1561 tcgatgacct cgtcctcctt gcccgagaga aacaggtcgg cgccgtggac atcctccgca
     1621 taatcctccg cccttatcag gccgacgcac ggtgcggtgc agcgccggat ctggtgaagc
     1681 aggcaagggc gggagcggtt ggcgaagacg gtgtcctcgc aggtgcggat acgaaacacc
     1741 ttttgcagca gttgaatgct ttcacgtacc gcgcctgcgt tgggaaacgg tccgtaatag
     1801 cggtgacgct tgtccagcgc cccgcggtgg aaccccaggc gtgggtaagc gtttcctgtc
     1861 acgaccaggt atgggtaggt tttatcgtct cggaacagta tgttatagcg tggacttaaa
     1921 gactttatta agttactctc cagcagcaag gcctcggctt cggagcgcgt aaccgtggtt
     1981 tccagggcag ccacctggcc gagcatgagc tggatgcggg gtgatagtcc tccagccttc
     2041 tgaaagtaag aggcgacccg cttcctgaga tcgagcgcct tgccgacata gagcacctcc
     2101 cccgcactgt tcaacatgcg gtagacgccg ggcatgtgag gcaacccgga gacgatggag
     2161 gcaggatcaa attccacggc ggctggaaat tgaaatcaga tatccgccga tggcgtcgaa
     2221 cacgttccgg aacatctccg gagtaagacg tttggtctgc gtattatagc gactgcagtg
     2281 gtagctatcg tagagcgcct gcccgcgggg aagcgcatgc cgggccccgt gccggaaccc
     2341 gcacgtggag ggcttgagac cgcacgccat aagcaccgca tggtgggcga tcagccccaa
     2401 cgccagtatt gccgcgttgt ccggcatcga gtgaatttcg gacgcaagat aatcgttgca
     2461 gtgacgaatc tcgccgggca tcggccgatt ctcgggaggc aggcatttga ccgcattcgt
     2521 aatacggcag ttcttcagcc gcagtccgtc ggtcgcgctg atcgataccg ggctgtccgc
     2581 gtagccatag cggtggagcg tgtcatagag caggatgccg gcatgatccc ccgtgaacgg
     2641 acgcccggtg cggttggccc cgtgcattcc tggtgcgagc ccgacgatca gtaactgtgc
     2701 ccgcgccggg ccgaaacagg cgaccgggcg cgcatgatag tccgggtatt cgcaacgcac
     2761 ccggtccagg tggcgagcca accgcgggca gcgccgacaa tcggtggaga attctcgcat
     2821 taatccaagc ggttagtgta aaatgatgcc cttttttcag gcagattaaa gaaatggcaa
     2881 aagttctggc aaccgaaatc cgcgccggca acctgatcga gtgggacaag cgagtttggc
     2941 gcgtgctcaa gtcctaccac gtccatgtcg gcggacgcgg gggcgccttc atgcaggtcg
     3001 aaatgaagga catcgagtcg ggcaccaagc gcaacgagcg catccgcacg gaagacaaga
     3061 tcgagcgcgc gttcgtggac cagcgcgaca tgcaatatct ctacagcgac gcctccggct
     3121 acatcttcat ggacaaggag tcgttcgagc agttgacgct gcccgccgac ttcctcgaag
     3181 gacaggccgg ttatctcatg cccaatgccg acgtgcaggt caattttcac aacgaccggc
     3241 cgatcggcgt gcaaatcccc tccaccgtga cgatgaccgt ggtcgaaacc gagcccaatc
     3301 tcaagggcgc gaccgcaacc ggctctttca agccggccaa gaccgagacc ggcgtcaccg
     3361 tgctggtgcc ccagtttgtc gcggaaggtg aaaaaatcaa ggtcaacacc gataccggcg
     3421 attacatcga gcgcgcgtag aagctaaccg cgcggacggt gcccgcgcgg ccacagctgg
     3481 atgaccgacg tttgacggcg tcccggcgct tcatggattt ccggttgcag cgtaatgtgt
     3541 tctattccgt gacgctcatg cagcagcacc cttgctgcgg cgagtatggc aggccattcc
     3601 cgcatctcga ccaactcgat gtgcgcggac aaggcgattc tgcccggcac gatgctccag
     3661 acatgcagat catgcaccga aaccacgccc ggcagacctg ccagccctcg tccgatttcg
     3721 cccaactcga gcgccggggg aaccccctcc atgagcacgt gcaaggtgtc gcgcagcagt
     3781 cttaacgtgc tgaagagtat cagcccggcg ataaccagcg acaggatagg gtcgatggga
     3841 agccacccgg tcatgtagat cacggctcct gccaccaggg cggcgaacga gctgacaagg
     3901 tcgctcatga catgcaacat cgctgcgcgc gagttaaggc ccggttcacc gcggctcatg
     3961 acccagatca ccgtcgcgtt gacgagcagg cccgccagcg ctatcagcat catggcagct
     4021 gccgctaccg gtccaggcgc gcgcaggcgg gataccgcct ccacgaccag caagaggacg
     4081 atggccagca tgagcacgct gttgaccagc gcaccgatga cttccgccct ctcccaacca
     4141 taggtgtgcc gctggccgga cggacgttgc gccagcctgg ctgcaatggc cgcgagcccc
     4201 agggcgaggg catcggagaa catgtggcca gcatcgctca acagcgcgag cgatccggtc
     4261 cacaatccgc ccgccagctc gatcacggca tagccggctg taatcgccag ggcgatgccg
     4321 aacgcgggac ggccgggttc gcgccgatgc gtatgatggg cgctcatgag ctcagcggca
     4381 gttcgccatc gcgcaagccg atgccggcga tcgcatgcag cgagcggctg taatgggcgt
     4441 cttcccgcag cttgaccatg ccctccgccc ggggccttcc atgcaggcgc gcgagccgcg
     4501 ccaggctgac cgcgggcatc cggtaggtca gcccctccag cagctggtcc gctcccgcgg
     4561 ggttattgca taacagagcc atatcgcaac ccgccgcgag cgccgcgttt gcgcggtcga
     4621 ccactccgcc gacaatactc gccccctcca tgctgagatc gtcgctgaac accacaccgt
     4681 tgaagccgag tgttccacgc agaacctgct tcagccagac cgcagaaaat ccggctggac
     4741 ggctgtccac gcggggataa atcacgtgag cgggcatgac accgccgaga cccgcgtcga
     4801 tcaggctccg gaacggcatg agatcagacg cttcgatctc ctcgaaattc cggtcatcga
     4861 ccggcacttc gtgatgggaa tcgccgcgca catgcccgtg gccgggaaaa tgcttcccga
     4921 ccccggccat gccggccctt ctcaacccct gaaacagtcc gcgcgccagt tcgacgaccg
     4981 cgtgtggatc ggagtgcagc gcgcggtctc caattacgct gctgttgccg taatcgacat
     5041 cgagcaccgg ggcaaagctc agatccacgc cgtgtgcgcg caattccgcc ccgaggacaa
     5101 atgccacttc gccagcgagc actcgggctc gctgcggatt ggcgtcccac agcctgccca
     5161 gttcccgcat cgggggtaac tccgtgaaac cttcccgaaa acgctgcacc ctgccgcctt
     5221 catgatcgac cgcgatgatg aggggcgggt tgcgaagcgc atgaatgtcg tcggtcagcc
     5281 gcatcagctg cggggaatcc gagtaattcc gggtgaacag gacgacgcct cccaccagcg
     5341 ggtgcagcag acggcggcgg tcatccgctg tgagcgcggt gccggcaacg tccagcatga
     5401 gtggtcccag cgccaacgtc acggtcttca tttttccagc actacgcagg cgcaagcgag
     5461 gctctgctcg tcggtgatcg tcaaatgatg gcttgcgatg ccgagaccat ttaccagctc
     5521 ctcgagtcgg ggatgaaagg tgaatcccgg tttgcctgcg gcgttctgga ccacgctgat
     5581 gcactgcagg gtcacgggat aacggaaacc cgtccccatg gctttggaga aggcctcttt
     5641 cgccgcgaac cggttggcca ggaacaatac cggcttcgct gtcttgaggt agcccgccca
     5701 ttccagctgg gtcagcacac ggcgcgcgaa gcgttcgccg tacttttcca gcagccgcga
     5761 gatgcgctgc ggttcaacga cgtcgatgcc tatgccgtag atcattgcgc ggaacccatg
     5821 agcgccttca tttcacgaac tgcctgcgcg atacccacga acaccgatcg ggcgacgatg
     5881 gcatggccga tgttgagctc gcgcatatgg gctatgccgg cgatcggccg gacgttcacg
     5941 taattcagcc catggccggc attgacgtgc agccccttcc cggttgcata ctcggtcgcg
     6001 gcgcggatcc ggtcgagtgc tttcctttgc tggcttgcgc tgcgtgcccc ggcataactt
     6061 ccggtatgta actcgattac cggggccccc gctttcagcg cggcatcgat ctgcttcctg
     6121 tcggcgtcaa tgaacaggga aacgcgaatg ccagcggcac ccaggtcacg gcacgcccgc
     6181 tccacctgct tgagcttgac tgccgcatcg aggccgccct cggtggtgag ttctgcccgc
     6241 cgttccggca ccaggcagac atcatgcggg cggaccctcc gtgcgaatgc gatcatttcc
     6301 ggggtgacgg ccgcctccag gttcatgcgc gtgccgagcg actcgcgcag cgccaggacg
     6361 tcccgatcct ggatatgccg gcggtcttcc cgcaaatgca gcgtaatgta gtccgcgcct
     6421 gccccctcgg cggcgagggc agcctccagc gggctggggt aatccgtccc gcgcgcctgc
     6481 cgcaatgtag cgacgtggtc gatattgacg cccagcagaa tcatat
//
LOCUS       DBNGSG010000513         3047 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05315
            SRR25158427_k127_1696924, whole genome shotgun sequence.
ACCESSION   DBNGSG010000513 DBNGSG010000000
VERSION     DBNGSG010000513.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414723
            Sequence Read Archive: SRR25158427
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 20:52:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,742
            CDSs (total)                      :: 2,716
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 2 of 22
            Pseudo Genes (incomplete)         :: 11 of 22
            Pseudo Genes (internal stop)      :: 9 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158427_k127_1696924"
                     /isolate="MAG05315"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.28111111 N 114.8736111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="AC158Q_13695"
     CDS             complement(<1..1043)
                     /locus_tag="AC158Q_13695"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HIR3340869.1"
                     /translation="MDDLTRYADRPLAFLGRYVRRHPYSHLTILMAVVGAVSCSVGSQ
                     YSVKFLVDTLSGNADGNARVWLAFTSLVLLLIGDNLLWRVAGWVASHAFVAVTGDLRS
                     DLFRYLTGHAPTYFQDRLPGTLASRVTATSNAVFIIENMSAWNVLPPILATIVAIAFL
                     ATVNAAMTLVMIAVAAAMAFILFRLAAAGRPLHRGFADKAAAVDGEMVDIIGNMPLVR
                     AFGGLRGEHLRFDDAVGGEMTARRRSLRYLEKLRLVHALATIVFTIALLAWSIVLWQR
                     GTATTGDVVLVCTLGFTILHATRDLAVALVDVTQHLARLSEAIAVILLPYELRDHPEA
                     APLGRRQGSVSFEG"
     gene            1268..2338
                     /locus_tag="AC158Q_13700"
     CDS             1268..2338
                     /locus_tag="AC158Q_13700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3340870.1"
                     /translation="MRIAQIAPLTEAIPPKLYGGTERVVSWLTEELVSLGHDITLFAS
                     GDSITAAELEPMWPKALRLDNSVLDPMSLHMLMLERVRRRARDFDCLHFHLDYFPFSL
                     FSRQPTPFITTLHGRLDLQEHQPVFTEFASVPVISISNAQRRPLSQAGWVRTIYHGMP
                     ERQLTPQPVTPQYLAFLGRIAPEKAVDRAIRIACEAGLPLRIAAKVDKVDREYFESQI
                     RPLLSLPNIDYIGEIDDTQKASFLSGAIALLMPIDWPEPFGLVMIEAMACGTPVVGFG
                     SGSVPEVVEDGLTGFVVGDEKSAVKAISRAADLPRGRIRKRFEQRFTARRMAEEYLEV
                     YRSLCEEETERPALAAVSGGRA"
     gene            complement(2360..2830)
                     /locus_tag="AC158Q_13705"
     CDS             complement(2360..2830)
                     /locus_tag="AC158Q_13705"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR3340871.1"
                     /translation="MTKPIRVGLISDTHGLVRDEAVAALKGCRHILHAGDIDTLDVLA
                     ELRKLAPVTVVRGNNDRGSWADAIPVYDVVEFGPVSVYLRHDEAELDIDPRAAGFRVM
                     MFGHSHKPRVETRGGVLFVNPGSAGPRRFRLPVAMGELLICGGHVDARIIELSA"
     gene            complement(2827..>3047)
                     /locus_tag="AC158Q_13710"
     CDS             complement(2827..>3047)
                     /locus_tag="AC158Q_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR3340872.1"
                     /translation="MKPGSATLVDAGMWLLTHRRERAMWWYRRLLMPLALRFQKRLAC
                     SPGMIDTDDVDEILLVCRKTPAVRPATS"
BASE COUNT          559 a          988 c         1003 g          497 t
ORIGIN      
        1 ccttcgaagg aaacggagcc ttgccgccgg ccgagaggcg ccgcctcggg atggtcgcgc
       61 agctcgtagg gcaggaggat gaccgcgatc gcttccgaaa gccgcgccag gtgctgcgtc
      121 acgtcgacca gcgctaccgc gaggtcgcgg gtggcatgca ggatcgtgaa cccgagcgtg
      181 caaaccagca cgacgtcccc ggtggtcgcc gtgcctctct gccagagaac gatcgaccag
      241 gccagcagcg cgatcgtgaa cacgatggtg gcgagcgcgt ggaccaggcg cagcttttcg
      301 agatagcgca ggctgcgccg ccgcgccgtc atttccccgc cgaccgcatc gtcgaagcgc
      361 aggtgctcgc cgcgcagccc gccgaacgcc cttaccaatg gcatgttgcc gatgatgtcg
      421 accatctcgc cgtcgaccgc cgccgccttg tcggcgaagc cccggtgcag ggggcggccg
      481 gccgcggcca ggcggaacag gatgaaggcc atcgcggcgg caaccgcgat catgacgagc
      541 gtcatcgccg cgttgaccgt tgccaggaac gcaatcgcca cgatcgtcgc cagtatcggc
      601 ggcagcacgt tccaggcgga catgttctcg atgatgaaaa ccgcattgga ggtcgcggtg
      661 acgcggctcg ccagcgtgcc gggcaggcgg tcctggaaat aagtcggcgc gtggccggtc
      721 aggtagcgaa acagatcgct gcgcaaatcg ccggtgaccg cgacgaaggc gtggctagca
      781 acccagcccg cgacgcgcca cagcaggttg tcgccgatca acaacaagac cagagaggtg
      841 aaagccagcc agacgcgcgc gttcccgtct gcattacccg acagcgtatc caccaggaac
      901 ttgacgctgt attgcgagcc tacagaacaa ctgacggctc ccaccaccgc catcaggatt
      961 gtaaggtggg agtaaggatg ccgcctgaca taacgcccca gaaacgcgag aggccggtcg
     1021 gcgtatcggg tgagatcatc catggaccct ggcgcgggcg cgcccgagtg gtgcggtcgg
     1081 gcaggtgacg ccccattcgg tagcaagacc tgcgcttgaa gcagagactt tttggacttt
     1141 ggcggggacg ggaaagttgc agcagagttt tccccggcgt gtgccccccc ggtatgggaa
     1201 ttgctagatg tatggcggtc ccatcaagat gaaaaacgaa ccccttgtaa cataggagaa
     1261 gagttgcatg cgtatcgcac agattgcacc actaacggaa gccattcccc cgaaactgta
     1321 cggcggcacg gagcgagtgg tttcctggct gaccgaggag ctggtgtcgc tgggtcacga
     1381 catcaccctg ttcgccagcg gcgattcgat cacggcggcc gagctcgagc cgatgtggcc
     1441 gaaggcgttg cggctcgata attcggttct cgaccccatg tcgttgcaca tgctgatgct
     1501 cgagcgcgtg cggcggcgcg cgcgtgattt cgattgcctg cacttccatc tggattattt
     1561 cccgttctcg ctgttctcgc gccagccgac acctttcatc accacgctgc acggccgcct
     1621 cgatctccag gagcaccagc ctgtcttcac tgagttcgcg tcggtgccgg taatctcgat
     1681 ctccaatgcc cagcggcggc cgctgtcgca agccggctgg gtgcgcacga tctatcacgg
     1741 catgcccgag cggcagctca cgccgcagcc ggtgacgccg caatacctcg cttttctcgg
     1801 ccgcatcgcg ccggaaaaag cagtcgatcg cgcgatccgg atcgcgtgcg aagccggcct
     1861 gccgctgcgc atcgcggcca aggtcgacaa ggtcgatcgc gaatattttg aatcgcagat
     1921 acggccattg ctgtccctgc cgaacatcga ctacatcggc gagatcgatg acacgcagaa
     1981 agcgtcgttc ctgagcggcg cgatcgcgct gctcatgccg atcgactggc cggaaccgtt
     2041 cggactggtg atgatcgagg cgatggcctg cggcacgccg gtcgtgggat tcggaagcgg
     2101 ctcggtgccg gaggtggtcg aagacgggct gaccggcttc gtggtcggcg acgagaagag
     2161 tgccgtcaag gcgatctcgc gggcggcgga cctgccgcgc ggccggatac gaaagcgttt
     2221 cgagcagcgc ttcaccgcgc gccgcatggc ggaggaatat ctcgaggtgt accgaagcct
     2281 gtgcgaggag gaaacggagc gcccggcgct ggcggccgtg tccggaggac gcgcatgacg
     2341 cggcgcgacc gggacagtct caggcgctga gctcgatgat ccgggcgtcg acgtgtccgc
     2401 cgcagatcag cagctctccc atcgcgaccg gcagcctgaa gcggcgcggg ccggcgctgc
     2461 cgggattgac gaacagcacg ccgccgcggg tctcgaccct cggcttgtgc gaatgcccga
     2521 acatcatgac ccggaagccc gccgcccgcg gatcgatgtc cagctccgct tcatcatgcc
     2581 ggagatagac ggataccggg ccgaattcca ccacgtcgta cacgggtatc gcatccgccc
     2641 acgatccccg gtcgttgttg ccgcggacca ccgtcaccgg cgcaagcttg cgcaactcgg
     2701 ccagcacgtc cagggtgtcg atgtcgcccg cgtgcaggat atgccggcat cccttcagcg
     2761 ccgcgacggc ctcgtcccgc acgagcccgt gcgtatcgga gatcaggccg acccggatcg
     2821 gcttcgtcat gaagttgccg ggcggacggc gggcgttttc cggcaaacga gcaggatttc
     2881 gtcgacatca tcggtgtcga tcattcccgg ggagcacgcc agccttttct ggaagcgcaa
     2941 cgccagcggc atgagcaggc gccggtacca ccacatggct ctctcgcgcc ggtgcgtgag
     3001 cagccacatg cccgcgtcga ccagcgtggc ggagcccggc ttcatgc
//
